Nphp3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nphp3
Entrez Gene ID	74025
Full Name	nephronophthisis 3 (adolescent)
Synonyms	3632410F03Rik,AI550417,C230078J01,D330020E01Rik,pcy
General protein information	Preferred Names nephronophthisis 3 (adolescent) Names nephrocystin-3
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9 F1\|9 56.11 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_028721.3	NP_082997.3	nephrocystin-3 isoform a
NM_172460.1	NP_766048.1	nephrocystin-3 isoform b

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Nphp3	NP_001178811.1
Homo sapiens (human)	NPHP3	NP_694972.3
Canis lupus familiaris (dog)	NPHP3	XP_003639875.1
Mus musculus (house mouse)	Nphp3	NP_082997.3
Pan troglodytes (chimpanzee)	NPHP3	XP_516758.4
Gallus gallus (chicken)	NPHP3	XP_418790.2
Xenopus tropicalis (tropical clawed frog)	nphp3	NP_001072733.2
Bos taurus (cattle)	NPHP3	NP_001179081.1
Danio rerio (zebrafish)	nphp3	NP_001189370.1

Gene Ontology
Bibliography

Related articles in PubMed

Green mamba peptide targets type-2 vasopressin receptor against polycystic kidney disease.
Ciolek J, Reinfrank H, Quinton L, Viengchareun S, Stura EA, Vera L, Sigismeau S, Mouillac B, Orcel H, Peigneur S, Tytgat J, Droctov? L, Beau F, Nevoux J, Lomb?s M, Mourier G, De Pauw E, Servent D, Mendre C, Witzgall R, Gilles N
Proceedings of the National Academy of Sciences of the United States of America114(27)7154-7159(2017 Jul)

microRNA-17 family promotes polycystic kidney disease progression through modulation of mitochondrial metabolism.
Hajarnis S, Lakhia R, Yheskel M, Williams D, Sorourian M, Liu X, Aboudehen K, Zhang S, Kersjes K, Galasso R, Li J, Kaimal V, Lockton S, Davis S, Flaten A, Johnson JA, Holland WL, Kusminski CM, Scherer PE, Harris PC, Trudel M, Wallace DP, Igarashi P, Lee EC, Androsavich JR, Patel V
Nature communications814395(2017 Feb)

Impairment of Wnt11 function leads to kidney tubular abnormalities and secondary glomerular cystogenesis.
Nagy II, Xu Q, Naillat F, Ali N, Miinalainen I, Samoylenko A, Vainio SJ
BMC developmental biology16(1)30(2016 08)

Cilia gene mutations cause atrioventricular septal defects by multiple mechanisms.
Burnicka-Turek O, Steimle JD, Huang W, Felker L, Kamp A, Kweon J, Peterson M, Reeves RH, Maslen CL, Gruber PJ, Yang XH, Shendure J, Moskowitz IP
Human molecular genetics25(14)3011-3028(2016 07)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Inv acts as a molecular anchor for Nphp3 and Nek8 in the proximal segment of primary cilia.
Title: Inv acts as a molecular anchor for Nphp3 and Nek8 in the proximal segment of primary cilia.

The pcy mutation generates a hypomorphic Nphp3 allele that is responsible for the cystic kidney disease phenotype, whereas complete loss of Nphp3 function results in situs inversus, congenital heart defects, and embryonic lethality in mice.
Title: Loss of nephrocystin-3 function can cause embryonic lethality, Meckel-Gruber-like syndrome, situs inversus, and renal-hepatic-pancreatic dysplasia.

a homozygous missense mutation in Nphp3 is probably responsible for the polycystic kidney disease (pcy) mouse phenotype
Title: Mutations in a novel gene, NPHP3, cause adolescent nephronophthisis, tapeto-retinal degeneration and hepatic fibrosis.

The following Nphp3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nphp3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu21112	NM_172460.1 Latest version!	Mus musculus nephronophthisis 3 (adolescent) (Nphp3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
OMu21113	NM_028721.3 Latest version!	Mus musculus nephronophthisis 3 (adolescent) (Nphp3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nphp3 ( NM_172460.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_766048.1 Mus musculus nephronophthisis 3 (adolescent) (Nphp3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu21112
Clone ID Related Accession (Same CDS sequence)	NM_172460.1
Accession Version	NM_172460.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Mus musculus(house mouse)
Product	nephrocystin-3 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK133217.1 and AK052281.1. Transcript Variant: This variant (2) uses a different splice site in the 5' CDS and lacks all of the downstream CDS, compared to variant 1. The resulting protein (isoform b) is much shorter and has a distinct C-terminus when it is compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK052281.1, AK048877.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849382 [ECO:0000348] ##Evidence-Data-END##

ATGGGCACAG CCTCGTCGCT GGTGAGTCCC ACTGGCGGGG AGGTGATCGA GGACACGTAC 
GGGGCAGGCG GCGGCGAGGC CTGCGAGATC CCGGTGGAAG TGAAACCCAA GGCCCGCCTG 
CTGCGCAGCT CGTTCCGCAG GGGCGCGGGA GCCGGGCCCG GGTCGCTGCC TCGCGCGGCA 
GGGGGCGGCG GGCTGCTGGG CGCCAGCTTC AAGTCCACCG GCTCGTCTGT GCCCGAGCTA 
GAGTATGCGG CGGCCGAGTT CGAGCGGCTC AAGAAAGAGT ATGAGATCTT CCGGGTCAGC 
AAGAACCAGG AGTTGCTGTC CATGGGCCGC CGAGAGGCCA AGCTGGACAC GGAGAACAAG 
CGGCTGCGGG CGGAGCTGCA GGCACTTCAG AAAACTTACC AGAAGATACT TCGGGAAAAA 
GAGGGTGCGT TAGAAGCAAA ATACCAAGCC ATGGAGAGAG CGGTCACATT TGAACATGAC 
AGAGACAGGG TTAAAAGGCA ATTCAAGATT TTTAGGGAAA CCAAAGAAAA TGAAATTCAG 
GACTTACTGA GGGCCAAGCG AGAGTTGGAG AGCAAACTTC AGAGGCTGCA GGCTCAGGGG 
ATCCAAGTGT TCGACCCTGG GGAGTCTGAC TCGGATGACA ACTGCACAGA TGTCACTGCT 
GCGGGAACGC AATGTGAGTA CTGGGCCAGC AGAGCCCTGG GCAGCGAGCA TTCCATAGGC 
AGCATGATCC AGCTCCCGCA GCCTTTCAGA GGCCCCGAGT TCGCCCACAG TTCTATAGAT 
GTGGAAGGAC CCTTTGCAAA CATCAACCGA GATGACTGGG ACGCTGCTGT AGCTGGTTTA 
TTACAGGCCA CACCCTTGTT CTCACATTCC CTGTGGAGTC ACCCTGTCAG GTGCTACCTC 
ATCTACACAG ATGAAACACA GCCCGAAATG GAACTGTTCC TTAAGGACCA TTCACCTAAA 
CTTAAAAGAA TGTGCGAGAC AATGGGATAT TTCTTCCTCG CTGTTTATTT TCCATTAGAT 
GTTGAAAATC AGTATCTCAC TGTAAGAAAA TGGGAAATTG AGAAGAGTTC TTTAGTTATT 
TTATTCCTTC ACTCAACATT ACCCAGTTTT TTACTGGAAG ACTGTGAAGA AGCCTTCCTG 
CAAAACCCAG AAGGAAAGCC AGGATTAATC TACCACCGTT TGGAGGATGG GAAAGTCACT 
TGTGACTCTG TCCAGCAGTT CCTGGATCAA GTTTCCAACC TAGGCAAGAC CACTAAAGCC 
AAGATCATTG AACACTCCGG AGATCCTGCA GAGGGAGTGT GTAAGATTTA CGTGGGTGTG 
GAAAAGATTA TTAAACAGGT AAATATGCGT TTCTCTGTTA AGTTTTCATC TAAAATAGCT 
TCATGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_766048.1
CDS	238..1626
Translation	MGTASSLVSPTGGEVIEDTYGAGGGEACEIPVEVKPKARLLRSSFRRGAGAGPGSLPRAAGGGGLLGASFKSTGSSVPELEYAAAEFERLKKEYEIFRVSKNQELLSMGRREAKLDTENKRLRAELQALQKTYQKILREKEGALEAKYQAMERAVTFEHDRDRVKRQFKIFRETKENEIQDLLRAKRELESKLQRLQAQGIQVFDPGESDSDDNCTDVTAAGTQCEYWASRALGSEHSIGSMIQLPQPFRGPEFAHSSIDVEGPFANINRDDWDAAVAGLLQATPLFSHSLWSHPVRCYLIYTDETQPEMELFLKDHSPKLKRMCETMGYFFLAVYFPLDVENQYLTVRKWEIEKSSLVILFLHSTLPSFLLEDCEEAFLQNPEGKPGLIYHRLEDGKVTCDSVQQFLDQVSNLGKTTKAKIIEHSGDPAEGVCKIYVGVEKIIKQVNMRFSVKFSSKIASW

Target ORF information:

RefSeq Version	NM_172460.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus nephronophthisis 3 (adolescent) (Nphp3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172460.1

ORF Insert Sequence:

ATGGGCACAG CCTCGTCGCT GGTGAGTCCC ACTGGCGGGG AGGTGATCGA GGACACGTAC 
GGGGCAGGCG GCGGCGAGGC CTGCGAGATC CCGGTGGAAG TGAAACCCAA GGCCCGCCTG 
CTGCGCAGCT CGTTCCGCAG GGGCGCGGGA GCCGGGCCCG GGTCGCTGCC TCGCGCGGCA 
GGGGGCGGCG GGCTGCTGGG CGCCAGCTTC AAGTCCACCG GCTCGTCTGT GCCCGAGCTA 
GAGTATGCGG CGGCCGAGTT CGAGCGGCTC AAGAAAGAGT ATGAGATCTT CCGGGTCAGC 
AAGAACCAGG AGTTGCTGTC CATGGGCCGC CGAGAGGCCA AGCTGGACAC GGAGAACAAG 
CGGCTGCGGG CGGAGCTGCA GGCACTTCAG AAAACTTACC AGAAGATACT TCGGGAAAAA 
GAGGGTGCGT TAGAAGCAAA ATACCAAGCC ATGGAGAGAG CGGTCACATT TGAACATGAC 
AGAGACAGGG TTAAAAGGCA ATTCAAGATT TTTAGGGAAA CCAAAGAAAA TGAAATTCAG 
GACTTACTGA GGGCCAAGCG AGAGTTGGAG AGCAAACTTC AGAGGCTGCA GGCTCAGGGG 
ATCCAAGTGT TCGACCCTGG GGAGTCTGAC TCGGATGACA ACTGCACAGA TGTCACTGCT 
GCGGGAACGC AATGTGAGTA CTGGGCCAGC AGAGCCCTGG GCAGCGAGCA TTCCATAGGC 
AGCATGATCC AGCTCCCGCA GCCTTTCAGA GGCCCCGAGT TCGCCCACAG TTCTATAGAT 
GTGGAAGGAC CCTTTGCAAA CATCAACCGA GATGACTGGG ACGCTGCTGT AGCTGGTTTA 
TTACAGGCCA CACCCTTGTT CTCACATTCC CTGTGGAGTC ACCCTGTCAG GTGCTACCTC 
ATCTACACAG ATGAAACACA GCCCGAAATG GAACTGTTCC TTAAGGACCA TTCACCTAAA 
CTTAAAAGAA TGTGCGAGAC AATGGGATAT TTCTTCCTCG CTGTTTATTT TCCATTAGAT 
GTTGAAAATC AGTATCTCAC TGTAAGAAAA TGGGAAATTG AGAAGAGTTC TTTAGTTATT 
TTATTCCTTC ACTCAACATT ACCCAGTTTT TTACTGGAAG ACTGTGAAGA AGCCTTCCTG 
CAAAACCCAG AAGGAAAGCC AGGATTAATC TACCACCGTT TGGAGGATGG GAAAGTCACT 
TGTGACTCTG TCCAGCAGTT CCTGGATCAA GTTTCCAACC TAGGCAAGAC CACTAAAGCC 
AAGATCATTG AACACTCCGG AGATCCTGCA GAGGGAGTGT GTAAGATTTA CGTGGGTGTG 
GAAAAGATTA TTAAACAGGT AAATATGCGT TTCTCTGTTA AGTTTTCATC TAAAATAGCT 
TCATGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nphp3 ( NM_028721.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_082997.3 Mus musculus nephronophthisis 3 (adolescent) (Nphp3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu21113
Clone ID Related Accession (Same CDS sequence)	NM_028721.3
Accession Version	NM_028721.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3978bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	nephrocystin-3 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK133217.1, AK173327.1 and BM219539.2. On Nov 2, 2007 this sequence version replaced NM_028721.2. Transcript Variant: This variant (1) represents the longer transcript and it encodes the longer protein (isoform a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY259499.1, AK173327.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849382 [ECO:0000348] ##Evidence-Data-END##

ATGGGCACAG CCTCGTCGCT GGTGAGTCCC ACTGGCGGGG AGGTGATCGA GGACACGTAC 
GGGGCAGGCG GCGGCGAGGC CTGCGAGATC CCGGTGGAAG TGAAACCCAA GGCCCGCCTG 
CTGCGCAGCT CGTTCCGCAG GGGCGCGGGA GCCGGGCCCG GGTCGCTGCC TCGCGCGGCA 
GGGGGCGGCG GGCTGCTGGG CGCCAGCTTC AAGTCCACCG GCTCGTCTGT GCCCGAGCTA 
GAGTATGCGG CGGCCGAGTT CGAGCGGCTC AAGAAAGAGT ATGAGATCTT CCGGGTCAGC 
AAGAACCAGG AGTTGCTGTC CATGGGCCGC CGAGAGGCCA AGCTGGACAC GGAGAACAAG 
CGGCTGCGGG CGGAGCTGCA GGCACTTCAG AAAACTTACC AGAAGATACT TCGGGAAAAA 
GAGGGTGCGT TAGAAGCAAA ATACCAAGCC ATGGAGAGAG CGGTCACATT TGAACATGAC 
AGAGACAGGG TTAAAAGGCA ATTCAAGATT TTTAGGGAAA CCAAAGAAAA TGAAATTCAG 
GACTTACTGA GGGCCAAGCG AGAGTTGGAG AGCAAACTTC AGAGGCTGCA GGCTCAGGGG 
ATCCAAGTGT TCGACCCTGG GGAGTCTGAC TCGGATGACA ACTGCACAGA TGTCACTGCT 
GCGGGAACGC AATGTGAGTA CTGGGCCAGC AGAGCCCTGG GCAGCGAGCA TTCCATAGGC 
AGCATGATCC AGCTCCCGCA GCCTTTCAGA GGCCCCGAGT TCGCCCACAG TTCTATAGAT 
GTGGAAGGAC CCTTTGCAAA CATCAACCGA GATGACTGGG ACGCTGCTGT AGCTGGTTTA 
TTACAGGCCA CACCCTTGTT CTCACATTCC CTGTGGAGTC ACCCTGTCAG GTGCTACCTC 
ATCTACACAG ATGAAACACA GCCCGAAATG GAACTGTTCC TTAAGGACCA TTCACCTAAA 
CTTAAAAGAA TGTGCGAGAC AATGGGATAT TTCTTCCTCG CTGTTTATTT TCCATTAGAT 
GTTGAAAATC AGTATCTCAC TGTAAGAAAA TGGGAAATTG AGAAGAGTTC TTTAGTTATT 
TTATTCCTTC ACTCAACATT ACCCAGTTTT TTACTGGAAG ACTGTGAAGA AGCCTTCCTG 
CAAAACCCAG AAGGAAAGCC AGGATTAATC TACCACCGTT TGGAGGATGG GAAAGTCACT 
TGTGACTCTG TCCAGCAGTT CCTGGATCAA GTTTCCAACC TAGGCAAGAC CACTAAAGCC 
AAGATCATTG AACACTCCGG AGATCCTGCA GAGGGAGTGT GTAAGATTTA CGTGGGTGTG 
GAAAAGATTA TTAAACAGGA CATCCTTGGC CTCGAGAACA CAGACGTGGA GGAGAAGGAT 
GGGGGCAGAG AGGATTCCAC TCCAGAAGAA GATGATTTCG GGGATGTTCT GTGGGACATA 
CACGATGAGC AAGAGCAGAT GGAAGCCTTT CAGCAGACTT CTAGCTCCGC CCATGAACTG 
GGATTCGAGA AGTATTACCA GCGCCTGGAT GATCTGGTGG TGGCGCCAGC CCCGATCCCA 
CCCCTCCTGG TTTCTGGCGG ACCAGGTTCT GGGAAGTCCC TTCTTTTATC AAAATGGATT 
CAGCTGCAGC AGAAGCATTT CCCAAACACC CTGATCCTGT CTCATTTTGT GGGGAGACCA 
ATGTCAACCA GCTCAGAGTC CTCTTTGATT ATCAAGCGGC TGACCCTGAA GCTGATGCAG 
CACTTCTGGG CAGTTTCTGC ACTGACACTG GATCCTGCTA AGCTTCTGGA GGAATTTCCA 
CATTGGCTGG AGAAGCTCTC TGCCCGGCAT CAGGGCAGCA TCATCATCAT CATCGATTCC 
ATAGATCAAG TTCAGCAAGT GGAGAAGCAC ATGAAGTGGC TGATAGACCC CCTGCCGGTG 
AATGTCAGAG TCATTGTTTC TGTGAACGTG GAGACCTGCC CCACAGCGTG GAGGTTGTGG 
CCTACACTGC ATCTTGATCC TTTAAGCCCC AAGGACGCCA TATCTATAAT AACAGCAGAA 
TGCTACTCCA TGGATGTGCG GTTGAGCAGA GAGCAGGAGA AGATGCTAGA ACAGCACTGC 
CGTCCAGCCA CAACACGCCA CGCCCTCTAT GTCACCCTTT TCAGCAAGAT GATGGCCTGT 
GCCGGGAGAG GGGGCAACGT AGCTGAAACC CTCCATCAGT GCCTGCAGTG TCAGGACACA 
GTGTCACTGT ACAAACTCGT TCTCCACCAC GTCCGGGAGT CCATGCCAAG TGACAGGGAT 
AAAGAGTGGA TGACACAGAT CCTCTGCCTC ATCAACGTTA GTCACAATGG CGTGAGTGAG 
TCAGAGCTGA TGGAGCTGTA TCCAGAGATG TCCTGGCTCT CTCTAACCTC CATCGTTCAC 
AGTTTACACA AAATGCACCT GCTGACTTAC AGCTGTGGCC TACTCAGGTT TCAGCATCTA 
CAGGCCTGGG AAACAGTGAG GCTGCAGTAC CTGGAGGACC CCGCCCTCGT GTCTTCATAT 
AGGGAAAAGC TCATTAGCTA TTTTGCCTCA CAGCTCAGTC AGGACAGAGT GACCTGGAGA 
AGCGCAGATG AGCTCCCATG GCTCTTCCAG CAGCAGGGGA GCAAACAGAA GCTGCACAGT 
TGCCTCCTCA ACCTCCTCGT GGCTCAGAAC CTTTACAAAA GAGGACACTT CGCCGAGTTG 
CTGAGTTACT GGCAGTTTGT AGGCAAAGAC AAAGGTGCCA TGGCGACCGA GTACTTTGAG 
TCACTGAAGC AGTATGAGAA CAGCGAAGGC GAGGAGAACA TGCTGTGCCT AGCCGACCTA 
TATGAAACCC TCGGCCGCTT TCTGAAGGAC CTAGGACTTC TCAGTCAGGC CGTGGTGCCT 
TTGCAGAGGT CCCTAGAAAT TCGGGAGACA GCCTTAGATC CTGATCACCC GAGAGTAGCC 
CAGTCCCTCC ACCAACTGGC CGGTGTGTAC GTGCAGTGGA AGAAGTTCGG GGATGCGGAG 
CAGCTGTATA AACAGGCCTT GGAGATCTCA GAAAACGCTT ATGGTGCAGA CCATCCGCAT 
GCTGCTCGTG AACTGGAGGC GCTGGCGACT CTGTACCACA AACAAAATAA GTACGAACAA 
GCTGAACATT TTAGGAAAAA ATCAGTTATA ATTCGTCAGC AAGCCACGAG GCGAAAAGGC 
AGCCTGTACG GGTTTGCCCT TCTACGCAGG CGGGCTCTGC AGTTGGAAGA GCTCACCTTG 
GGCAAGGACA AGCCTGAGAA CGCCCGCACC CTCAATGAGC TGGGTGTTCT CTACTTCCTT 
CAAAATAACC TCGAGACAGC AGAGCAGTTT CTGAAGCGTT CCTTGGAAAT GAGGGAGCGG 
GTTCTGGGGC CAGATCACCC AGACTGCGCA CAGTCCCTGA ATAACCTGGC AGCTCTTTGC 
AATGAGAAGA AGCAGTACGA GAAAGCAGAG GAGCTGTATG AGCGCGCCCT GGACATCCGG 
AGACGCGCAC TGGCTCCGGA CCACCCTTCC CTGGCCTACA CCGTGAAGCA CCTTGCAATC 
TTGTATAAGA AAACGGGAAA AGTTGACAAA GCTGTACCTC TGTATGAATT GGCTGTTGAA 
ATTCGGCAGA AATCCTTTGG CCCAAAGCAC CCCAGTGTAG CTACTGCCTT GGTAAACTTG 
GCAGTCCTTC ATAGCCAAAT GAAAAAGCAC AGTGAAGCCC TGCCACTATA TGAAAGAGCA 
TTAAAGATTT ATGAAGATAG CTTGGGGAGA ATGCATCCTC GAGTTGGAGA AACATTGAAA 
AATTTAGCTG TGCTCAGCTA TGAAGAAGGG AATTTTGAAA AGGCTGCTGA ACTGTACAAA 
AGGGCAATGG AGATAAAAGA AGCAGAGACA TCACTTTTGG GTGGAAAAGC TCCTTCAAGG 
CAGTCATCAA GTGGAGACAC ATTTCTCTTT AAAACAACTC ATTCCCCTAA CGTTTTTCTT 
CCCCAAGGGC AAAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_082997.3
CDS	238..4215
Translation	MGTASSLVSPTGGEVIEDTYGAGGGEACEIPVEVKPKARLLRSSFRRGAGAGPGSLPRAAGGGGLLGASFKSTGSSVPELEYAAAEFERLKKEYEIFRVSKNQELLSMGRREAKLDTENKRLRAELQALQKTYQKILREKEGALEAKYQAMERAVTFEHDRDRVKRQFKIFRETKENEIQDLLRAKRELESKLQRLQAQGIQVFDPGESDSDDNCTDVTAAGTQCEYWASRALGSEHSIGSMIQLPQPFRGPEFAHSSIDVEGPFANINRDDWDAAVAGLLQATPLFSHSLWSHPVRCYLIYTDETQPEMELFLKDHSPKLKRMCETMGYFFLAVYFPLDVENQYLTVRKWEIEKSSLVILFLHSTLPSFLLEDCEEAFLQNPEGKPGLIYHRLEDGKVTCDSVQQFLDQVSNLGKTTKAKIIEHSGDPAEGVCKIYVGVEKIIKQDILGLENTDVEEKDGGREDSTPEEDDFGDVLWDIHDEQEQMEAFQQTSSSAHELGFEKYYQRLDDLVVAPAPIPPLLVSGGPGSGKSLLLSKWIQLQQKHFPNTLILSHFVGRPMSTSSESSLIIKRLTLKLMQHFWAVSALTLDPAKLLEEFPHWLEKLSARHQGSIIIIIDSIDQVQQVEKHMKWLIDPLPVNVRVIVSVNVETCPTAWRLWPTLHLDPLSPKDAISIITAECYSMDVRLSREQEKMLEQHCRPATTRHALYVTLFSKMMACAGRGGNVAETLHQCLQCQDTVSLYKLVLHHVRESMPSDRDKEWMTQILCLINVSHNGVSESELMELYPEMSWLSLTSIVHSLHKMHLLTYSCGLLRFQHLQAWETVRLQYLEDPALVSSYREKLISYFASQLSQDRVTWRSADELPWLFQQQGSKQKLHSCLLNLLVAQNLYKRGHFAELLSYWQFVGKDKGAMATEYFESLKQYENSEGEENMLCLADLYETLGRFLKDLGLLSQAVVPLQRSLEIRETALDPDHPRVAQSLHQLAGVYVQWKKFGDAEQLYKQALEISENAYGADHPHAARELEALATLYHKQNKYEQAEHFRKKSVIIRQQATRRKGSLYGFALLRRRALQLEELTLGKDKPENARTLNELGVLYFLQNNLETAEQFLKRSLEMRERVLGPDHPDCAQSLNNLAALCNEKKQYEKAEELYERALDIRRRALAPDHPSLAYTVKHLAILYKKTGKVDKAVPLYELAVEIRQKSFGPKHPSVATALVNLAVLHSQMKKHSEALPLYERALKIYEDSLGRMHPRVGETLKNLAVLSYEEGNFEKAAELYKRAMEIKEAETSLLGGKAPSRQSSSGDTFLFKTTHSPNVFLPQGQS

Target ORF information:

RefSeq Version	NM_028721.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus nephronophthisis 3 (adolescent) (Nphp3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_028721.3

ORF Insert Sequence:

ATGGGCACAG CCTCGTCGCT GGTGAGTCCC ACTGGCGGGG AGGTGATCGA GGACACGTAC 
GGGGCAGGCG GCGGCGAGGC CTGCGAGATC CCGGTGGAAG TGAAACCCAA GGCCCGCCTG 
CTGCGCAGCT CGTTCCGCAG GGGCGCGGGA GCCGGGCCCG GGTCGCTGCC TCGCGCGGCA 
GGGGGCGGCG GGCTGCTGGG CGCCAGCTTC AAGTCCACCG GCTCGTCTGT GCCCGAGCTA 
GAGTATGCGG CGGCCGAGTT CGAGCGGCTC AAGAAAGAGT ATGAGATCTT CCGGGTCAGC 
AAGAACCAGG AGTTGCTGTC CATGGGCCGC CGAGAGGCCA AGCTGGACAC GGAGAACAAG 
CGGCTGCGGG CGGAGCTGCA GGCACTTCAG AAAACTTACC AGAAGATACT TCGGGAAAAA 
GAGGGTGCGT TAGAAGCAAA ATACCAAGCC ATGGAGAGAG CGGTCACATT TGAACATGAC 
AGAGACAGGG TTAAAAGGCA ATTCAAGATT TTTAGGGAAA CCAAAGAAAA TGAAATTCAG 
GACTTACTGA GGGCCAAGCG AGAGTTGGAG AGCAAACTTC AGAGGCTGCA GGCTCAGGGG 
ATCCAAGTGT TCGACCCTGG GGAGTCTGAC TCGGATGACA ACTGCACAGA TGTCACTGCT 
GCGGGAACGC AATGTGAGTA CTGGGCCAGC AGAGCCCTGG GCAGCGAGCA TTCCATAGGC 
AGCATGATCC AGCTCCCGCA GCCTTTCAGA GGCCCCGAGT TCGCCCACAG TTCTATAGAT 
GTGGAAGGAC CCTTTGCAAA CATCAACCGA GATGACTGGG ACGCTGCTGT AGCTGGTTTA 
TTACAGGCCA CACCCTTGTT CTCACATTCC CTGTGGAGTC ACCCTGTCAG GTGCTACCTC 
ATCTACACAG ATGAAACACA GCCCGAAATG GAACTGTTCC TTAAGGACCA TTCACCTAAA 
CTTAAAAGAA TGTGCGAGAC AATGGGATAT TTCTTCCTCG CTGTTTATTT TCCATTAGAT 
GTTGAAAATC AGTATCTCAC TGTAAGAAAA TGGGAAATTG AGAAGAGTTC TTTAGTTATT 
TTATTCCTTC ACTCAACATT ACCCAGTTTT TTACTGGAAG ACTGTGAAGA AGCCTTCCTG 
CAAAACCCAG AAGGAAAGCC AGGATTAATC TACCACCGTT TGGAGGATGG GAAAGTCACT 
TGTGACTCTG TCCAGCAGTT CCTGGATCAA GTTTCCAACC TAGGCAAGAC CACTAAAGCC 
AAGATCATTG AACACTCCGG AGATCCTGCA GAGGGAGTGT GTAAGATTTA CGTGGGTGTG 
GAAAAGATTA TTAAACAGGA CATCCTTGGC CTCGAGAACA CAGACGTGGA GGAGAAGGAT 
GGGGGCAGAG AGGATTCCAC TCCAGAAGAA GATGATTTCG GGGATGTTCT GTGGGACATA 
CACGATGAGC AAGAGCAGAT GGAAGCCTTT CAGCAGACTT CTAGCTCCGC CCATGAACTG 
GGATTCGAGA AGTATTACCA GCGCCTGGAT GATCTGGTGG TGGCGCCAGC CCCGATCCCA 
CCCCTCCTGG TTTCTGGCGG ACCAGGTTCT GGGAAGTCCC TTCTTTTATC AAAATGGATT 
CAGCTGCAGC AGAAGCATTT CCCAAACACC CTGATCCTGT CTCATTTTGT GGGGAGACCA 
ATGTCAACCA GCTCAGAGTC CTCTTTGATT ATCAAGCGGC TGACCCTGAA GCTGATGCAG 
CACTTCTGGG CAGTTTCTGC ACTGACACTG GATCCTGCTA AGCTTCTGGA GGAATTTCCA 
CATTGGCTGG AGAAGCTCTC TGCCCGGCAT CAGGGCAGCA TCATCATCAT CATCGATTCC 
ATAGATCAAG TTCAGCAAGT GGAGAAGCAC ATGAAGTGGC TGATAGACCC CCTGCCGGTG 
AATGTCAGAG TCATTGTTTC TGTGAACGTG GAGACCTGCC CCACAGCGTG GAGGTTGTGG 
CCTACACTGC ATCTTGATCC TTTAAGCCCC AAGGACGCCA TATCTATAAT AACAGCAGAA 
TGCTACTCCA TGGATGTGCG GTTGAGCAGA GAGCAGGAGA AGATGCTAGA ACAGCACTGC 
CGTCCAGCCA CAACACGCCA CGCCCTCTAT GTCACCCTTT TCAGCAAGAT GATGGCCTGT 
GCCGGGAGAG GGGGCAACGT AGCTGAAACC CTCCATCAGT GCCTGCAGTG TCAGGACACA 
GTGTCACTGT ACAAACTCGT TCTCCACCAC GTCCGGGAGT CCATGCCAAG TGACAGGGAT 
AAAGAGTGGA TGACACAGAT CCTCTGCCTC ATCAACGTTA GTCACAATGG CGTGAGTGAG 
TCAGAGCTGA TGGAGCTGTA TCCAGAGATG TCCTGGCTCT CTCTAACCTC CATCGTTCAC 
AGTTTACACA AAATGCACCT GCTGACTTAC AGCTGTGGCC TACTCAGGTT TCAGCATCTA 
CAGGCCTGGG AAACAGTGAG GCTGCAGTAC CTGGAGGACC CCGCCCTCGT GTCTTCATAT 
AGGGAAAAGC TCATTAGCTA TTTTGCCTCA CAGCTCAGTC AGGACAGAGT GACCTGGAGA 
AGCGCAGATG AGCTCCCATG GCTCTTCCAG CAGCAGGGGA GCAAACAGAA GCTGCACAGT 
TGCCTCCTCA ACCTCCTCGT GGCTCAGAAC CTTTACAAAA GAGGACACTT CGCCGAGTTG 
CTGAGTTACT GGCAGTTTGT AGGCAAAGAC AAAGGTGCCA TGGCGACCGA GTACTTTGAG 
TCACTGAAGC AGTATGAGAA CAGCGAAGGC GAGGAGAACA TGCTGTGCCT AGCCGACCTA 
TATGAAACCC TCGGCCGCTT TCTGAAGGAC CTAGGACTTC TCAGTCAGGC CGTGGTGCCT 
TTGCAGAGGT CCCTAGAAAT TCGGGAGACA GCCTTAGATC CTGATCACCC GAGAGTAGCC 
CAGTCCCTCC ACCAACTGGC CGGTGTGTAC GTGCAGTGGA AGAAGTTCGG GGATGCGGAG 
CAGCTGTATA AACAGGCCTT GGAGATCTCA GAAAACGCTT ATGGTGCAGA CCATCCGCAT 
GCTGCTCGTG AACTGGAGGC GCTGGCGACT CTGTACCACA AACAAAATAA GTACGAACAA 
GCTGAACATT TTAGGAAAAA ATCAGTTATA ATTCGTCAGC AAGCCACGAG GCGAAAAGGC 
AGCCTGTACG GGTTTGCCCT TCTACGCAGG CGGGCTCTGC AGTTGGAAGA GCTCACCTTG 
GGCAAGGACA AGCCTGAGAA CGCCCGCACC CTCAATGAGC TGGGTGTTCT CTACTTCCTT 
CAAAATAACC TCGAGACAGC AGAGCAGTTT CTGAAGCGTT CCTTGGAAAT GAGGGAGCGG 
GTTCTGGGGC CAGATCACCC AGACTGCGCA CAGTCCCTGA ATAACCTGGC AGCTCTTTGC 
AATGAGAAGA AGCAGTACGA GAAAGCAGAG GAGCTGTATG AGCGCGCCCT GGACATCCGG 
AGACGCGCAC TGGCTCCGGA CCACCCTTCC CTGGCCTACA CCGTGAAGCA CCTTGCAATC 
TTGTATAAGA AAACGGGAAA AGTTGACAAA GCTGTACCTC TGTATGAATT GGCTGTTGAA 
ATTCGGCAGA AATCCTTTGG CCCAAAGCAC CCCAGTGTAG CTACTGCCTT GGTAAACTTG 
GCAGTCCTTC ATAGCCAAAT GAAAAAGCAC AGTGAAGCCC TGCCACTATA TGAAAGAGCA 
TTAAAGATTT ATGAAGATAG CTTGGGGAGA ATGCATCCTC GAGTTGGAGA AACATTGAAA 
AATTTAGCTG TGCTCAGCTA TGAAGAAGGG AATTTTGAAA AGGCTGCTGA ACTGTACAAA 
AGGGCAATGG AGATAAAAGA AGCAGAGACA TCACTTTTGG GTGGAAAAGC TCCTTCAAGG 
CAGTCATCAA GTGGAGACAC ATTTCTCTTT AAAACAACTC ATTCCCCTAA CGTTTTTCTT 
CCCCAAGGGC AAAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Green mamba peptide targets type-2 vasopressin receptor against polycystic kidney disease.
Proceedings of the National Academy of Sciences of the United States of America114(27)7154-7159(2017 Jul)
Ciolek J,Reinfrank H,Quinton L,Viengchareun S,Stura EA,Vera L,Sigismeau S,Mouillac B,Orcel H,Peigneur S,Tytgat J,Droctov? L,Beau F,Nevoux J,Lomb?s M,Mourier G,De Pauw E,Servent D,Mendre C,Witzgall R,Gilles N

microRNA-17 family promotes polycystic kidney disease progression through modulation of mitochondrial metabolism.
Nature communications814395(2017 Feb)
Hajarnis S,Lakhia R,Yheskel M,Williams D,Sorourian M,Liu X,Aboudehen K,Zhang S,Kersjes K,Galasso R,Li J,Kaimal V,Lockton S,Davis S,Flaten A,Johnson JA,Holland WL,Kusminski CM,Scherer PE,Harris PC,Trudel M,Wallace DP,Igarashi P,Lee EC,Androsavich JR,Patel V

Impairment of Wnt11 function leads to kidney tubular abnormalities and secondary glomerular cystogenesis.
BMC developmental biology16(1)30(2016 08)
Nagy II,Xu Q,Naillat F,Ali N,Miinalainen I,Samoylenko A,Vainio SJ

Cilia gene mutations cause atrioventricular septal defects by multiple mechanisms.
Human molecular genetics25(14)3011-3028(2016 07)
Burnicka-Turek O,Steimle JD,Huang W,Felker L,Kamp A,Kweon J,Peterson M,Reeves RH,Maslen CL,Gruber PJ,Yang XH,Shendure J,Moskowitz IP

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Nphp3 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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