B3gat3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	B3gat3
Entrez Gene ID	72727
Full Name	beta-1,3-glucuronyltransferase 3 (glucuronosyltransferase I)
Synonyms	2810405M13Rik
General protein information	Preferred Names beta-1,3-glucuronyltransferase 3 (glucuronosyltransferase I) Names galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3 UDP-GlcUA:Gal beta-1,3-Gal-R glucuronyltransferase glcAT-I glcUAT-I glucuronosyltransferase I glucuronyltransferase I
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 19 Map Location: 19\|19 A

mRNA and Protein(s)

mRNA	Protein	Name
NM_024256.2	NP_077218.1	galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	B3GAT3	NP_001229555.1
Canis lupus familiaris (dog)	B3GAT3	XP_540906.2
Bos taurus (cattle)	B3GAT3	NP_991374.2
Homo sapiens (human)	B3GAT3	NP_036332.2
Mus musculus (house mouse)	B3gat3	NP_077218.1
Rattus norvegicus (Norway rat)	B3gat3	NP_001121656.1
Danio rerio (zebrafish)	b3gat3	NP_001015059.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002801	XP_312113.5
Drosophila melanogaster (fruit fly)	GlcAT-I	NP_726910.1
Caenorhabditis elegans (roundworm)	sqv-8	NP_496076.1
Xenopus tropicalis (tropical clawed frog)	b3gat3	NP_001025520.1

Gene Ontology
Bibliography

Related articles in PubMed

Faulty initiation of proteoglycan synthesis causes cardiac and joint defects.
Baasanjav S, Al-Gazali L, Hashiguchi T, Mizumoto S, Fischer B, Horn D, Seelow D, Ali BR, Aziz SA, Langer R, Saleh AA, Becker C, N?rnberg G, Cantagrel V, Gleeson JG, Gomez D, Michel JB, Stricker S, Lindner TH, N?rnberg P, Sugahara K, Mundlos S, Hoffmann K
American journal of human genetics89(1)15-27(2011 Jul)

Impairment of embryonic cell division and glycosaminoglycan biosynthesis in glucuronyltransferase-I-deficient mice.
Izumikawa T, Kanagawa N, Watamoto Y, Okada M, Saeki M, Sakano M, Sugahara K, Sugihara K, Asano M, Kitagawa H
The Journal of biological chemistry285(16)12190-6(2010 Apr)

Mice deficient in glucuronyltransferase-I.
Izumikawa T, Kitagawa H
Progress in molecular biology and translational science9319-34(2010)

A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58.
Yokoyama S, Ito Y, Ueno-Kudoh H, Shimizu H, Uchibe K, Albini S, Mitsuoka K, Miyaki S, Kiso M, Nagai A, Hikata T, Osada T, Fukuda N, Yamashita S, Harada D, Mezzano V, Kasai M, Puri PL, Hayashizaki Y, Okado H, Hashimoto M, Asahara H
Developmental cell17(6)836-48(2009 Dec)

Characterization of the mouse integrin subunit alpha10 gene and comparison with its human homologue. Genomic structure, chromosomal localization and identification of splice variants.
Bengtsson T, Camper L, Schneller M, Lundgren-Akerlund E
Matrix biology : journal of the International Society for Matrix Biology20(8)565-76(2001 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

mice with a deletion of GlcAT-I showed remarkable reduction of the synthesis of chondroitin sulfate and heparan sulfate and embryonic lethality before the 8-cell stage because of failed cytokinesis
Title: Impairment of embryonic cell division and glycosaminoglycan biosynthesis in glucuronyltransferase-I-deficient mice.

The following B3gat3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the B3gat3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu05501	NM_024256.2 Latest version!	Mus musculus beta-1,3-glucuronyltransferase 3 (glucuronosyltransferase I) (B3gat3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

B3gat3 ( NM_024256.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_077218.1 Mus musculus beta-1,3-glucuronyltransferase 3 (glucuronosyltransferase I) (B3gat3), mRNA.

Price & Availability↑

CloneID	OMu05501
Clone ID Related Accession (Same CDS sequence)	NM_024256.2
Accession Version	NM_024256.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC004038.1. On Jun 19, 2003 this sequence version replaced NM_024256.1. ##Evidence-Data-START## Transcript exon combination :: BC004038.1, AK144402.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGAAGCTGA AGCTGAAGAA CGTGTTTCTT GCCTACTTCC TGGTGTCGAT CGCCGGCCTC 
CTCTACGCTC TGGTGCAGCT CGGCCAGCCT TGCGACTGCC TCCCTCCGCT TCGAGCTGCA 
GCTGAGCAGC TTCGGCAGAA GGACCTGAGG ATATCCCAGT TGCAAGCTGA TCTCCGTCGC 
CCACCCCCTG TCCCAGCCCA GCCCCCTGAA CCTGAGGCCC TGCCTACTAT CTATGTCATT 
ACCCCCACCT ACGCCAGGCT GGTACAAAAG GCAGAGCTGG TTCGGCTGTC CCAGACCCTG 
AGCTTGGTGC CCCGTCTACA CTGGCTGCTA GTGGAGGACG CTGAGAGCCC TACCCCGCTG 
GTCTCGGGGC TGTTGGCCGC CTCTGGTCTC CTCTTTACAC ACCTGGCTGT CCTTACCCCC 
AAGGCTCAAC GGCTTAGGGA AGGTGAGCCA GGCTGGGTCC GGCCCCGAGG AGTGGAACAG 
CGCAATAAGG CCCTCGACTG GCTCCGAGGA AAAGGGGGTG CTGTTGGGGG GGAGAAGGAT 
CCACCGCCAC CAGGGACCCA AGGAGTCGTG TATTTTGCTG ACGATGACAA CACCTACAGC 
CGGGAGCTCT TTAAGGAGAT GCGTTGGACT CGCGGTGTCT CAGTGTGGCC TGTGGGGCTG 
GTGGGTGGCC TGCGATTTGA AGGTCCTCAG GTACAGGATG GCCGCGTTGT GGGTTTCCAC 
ACAGCATGGG AACCCAACAG GCCCTTTCCC TTGGACATGG CGGGATTTGC GGTTGCCCTG 
CCCTTGCTAT TGGCTAAGCC CAATGCCCAG TTTGATGCTA CTGCACCCCG GGGCCACCTG 
GAAAGTAGTC TCCTGAGCCA CCTTGTAGAT CCCAAGGACC TGGAGCCACG GGCTGCCAAT 
TGTACTCAGG TACTGGTATG GCACACCCGG ACAGAGAAAC CTAAGATGAA GCAGGAGGAG 
CAGCTACAAC GGCAGGGCCA GGGCTCAGAC CCAGCCATTG AGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_077218.1
CDS	175..1182
Translation	MKLKLKNVFLAYFLVSIAGLLYALVQLGQPCDCLPPLRAAAEQLRQKDLRISQLQADLRRPPPVPAQPPEPEALPTIYVITPTYARLVQKAELVRLSQTLSLVPRLHWLLVEDAESPTPLVSGLLAASGLLFTHLAVLTPKAQRLREGEPGWVRPRGVEQRNKALDWLRGKGGAVGGEKDPPPPGTQGVVYFADDDNTYSRELFKEMRWTRGVSVWPVGLVGGLRFEGPQVQDGRVVGFHTAWEPNRPFPLDMAGFAVALPLLLAKPNAQFDATAPRGHLESSLLSHLVDPKDLEPRAANCTQVLVWHTRTEKPKMKQEEQLQRQGQGSDPAIEV

Target ORF information:

RefSeq Version	NM_024256.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus beta-1,3-glucuronyltransferase 3 (glucuronosyltransferase I) (B3gat3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024256.2

ORF Insert Sequence:

ATGAAGCTGA AGCTGAAGAA CGTGTTTCTT GCCTACTTCC TGGTGTCGAT CGCCGGCCTC 
CTCTACGCTC TGGTGCAGCT CGGCCAGCCT TGCGACTGCC TCCCTCCGCT TCGAGCTGCA 
GCTGAGCAGC TTCGGCAGAA GGACCTGAGG ATATCCCAGT TGCAAGCTGA TCTCCGTCGC 
CCACCCCCTG TCCCAGCCCA GCCCCCTGAA CCTGAGGCCC TGCCTACTAT CTATGTCATT 
ACCCCCACCT ACGCCAGGCT GGTACAAAAG GCAGAGCTGG TTCGGCTGTC CCAGACCCTG 
AGCTTGGTGC CCCGTCTACA CTGGCTGCTA GTGGAGGACG CTGAGAGCCC TACCCCGCTG 
GTCTCGGGGC TGTTGGCCGC CTCTGGTCTC CTCTTTACAC ACCTGGCTGT CCTTACCCCC 
AAGGCTCAAC GGCTTAGGGA AGGTGAGCCA GGCTGGGTCC GGCCCCGAGG AGTGGAACAG 
CGCAATAAGG CCCTCGACTG GCTCCGAGGA AAAGGGGGTG CTGTTGGGGG GGAGAAGGAT 
CCACCGCCAC CAGGGACCCA AGGAGTCGTG TATTTTGCTG ACGATGACAA CACCTACAGC 
CGGGAGCTCT TTAAGGAGAT GCGTTGGACT CGCGGTGTCT CAGTGTGGCC TGTGGGGCTG 
GTGGGTGGCC TGCGATTTGA AGGTCCTCAG GTACAGGATG GCCGCGTTGT GGGTTTCCAC 
ACAGCATGGG AACCCAACAG GCCCTTTCCC TTGGACATGG CGGGATTTGC GGTTGCCCTG 
CCCTTGCTAT TGGCTAAGCC CAATGCCCAG TTTGATGCTA CTGCACCCCG GGGCCACCTG 
GAAAGTAGTC TCCTGAGCCA CCTTGTAGAT CCCAAGGACC TGGAGCCACG GGCTGCCAAT 
TGTACTCAGG TACTGGTATG GCACACCCGG ACAGAGAAAC CTAAGATGAA GCAGGAGGAG 
CAGCTACAAC GGCAGGGCCA GGGCTCAGAC CCAGCCATTG AGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Faulty initiation of proteoglycan synthesis causes cardiac and joint defects.
American journal of human genetics89(1)15-27(2011 Jul)
Baasanjav S,Al-Gazali L,Hashiguchi T,Mizumoto S,Fischer B,Horn D,Seelow D,Ali BR,Aziz SA,Langer R,Saleh AA,Becker C,N?rnberg G,Cantagrel V,Gleeson JG,Gomez D,Michel JB,Stricker S,Lindner TH,N?rnberg P,Sugahara K,Mundlos S,Hoffmann K

Impairment of embryonic cell division and glycosaminoglycan biosynthesis in glucuronyltransferase-I-deficient mice.
The Journal of biological chemistry285(16)12190-6(2010 Apr)
Izumikawa T,Kanagawa N,Watamoto Y,Okada M,Saeki M,Sakano M,Sugahara K,Sugihara K,Asano M,Kitagawa H

Mice deficient in glucuronyltransferase-I.
Progress in molecular biology and translational science9319-34(2010)
Izumikawa T,Kitagawa H

A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58.
Developmental cell17(6)836-48(2009 Dec)
Yokoyama S,Ito Y,Ueno-Kudoh H,Shimizu H,Uchibe K,Albini S,Mitsuoka K,Miyaki S,Kiso M,Nagai A,Hikata T,Osada T,Fukuda N,Yamashita S,Harada D,Mezzano V,Kasai M,Puri PL,Hayashizaki Y,Okado H,Hashimoto M,Asahara H

Characterization of the mouse integrin subunit alpha10 gene and comparison with its human homologue. Genomic structure, chromosomal localization and identification of splice variants.
Matrix biology : journal of the International Society for Matrix Biology20(8)565-76(2001 Dec)
Bengtsson T,Camper L,Schneller M,Lundgren-Akerlund E

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B3gat3 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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