gRNAs
The following Lin9 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Lin9 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OMu23358 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001103182.2 | |
| Accession Version | NM_001103182.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1677bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-10-21 | |
| Organism | Mus musculus(house mouse) | |
| Product | protein lin-9 homolog | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC167020.3, BC088992.1 and BC069181.1. On May 20, 2014 this sequence version replaced NM_001103182.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the functional protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK089989.1, AF190325.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849383 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGCACCGGG GCGGGCAGCC TTTGAAAAAG CGGCGCGGCT CGTTCAAGAT GGCGGAGCTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001096652.2 |
| CDS | 170..1846 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_001103182.2 |
1 | ATGCACCGGG GCGGGCAGCC TTTGAAAAAG CGGCGCGGCT CGTTCAAGAT GGCGGAGCTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu28839 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006496988.3 , XM_006496987.1 | |
| Accession Version | XM_006496988.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1473bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-08-07 | |
| Organism | Mus musculus(house mouse) | |
| Product | protein lin-9 homolog isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006496988.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGCCTTTCA GAAATTCAAA AAGAAGTCGA CTCTTTTCTG ATGAAGATGA CAGACAAATA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_006497051.1 |
| CDS | 289..1761 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_006496988.3 |
1 | ATGCCTTTCA GAAATTCAAA AAGAAGTCGA CTCTTTTCTG ATGAAGATGA CAGACAAATA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu28839 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006496988.3 , XM_006496987.1 | |
| Accession Version | XM_006496987.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1473bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-08-07 | |
| Organism | Mus musculus(house mouse) | |
| Product | protein lin-9 homolog isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGCCTTTCA GAAATTCAAA AAGAAGTCGA CTCTTTTCTG ATGAAGATGA CAGACAAATA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_006497050.1 |
| CDS | 353..1825 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_006496987.1 |
1 | ATGCCTTTCA GAAATTCAAA AAGAAGTCGA CTCTTTTCTG ATGAAGATGA CAGACAAATA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
An important role for Myb-MuvB and its target gene KIF23 in a mouse model of lung adenocarcinoma. |
LIN9, a subunit of the DREAM complex, regulates mitotic gene expression and proliferation of embryonic stem cells. |
Loss of LIN9, a member of the DREAM complex, cooperates with SV40 large T antigen to induce genomic instability and anchorage-independent growth. |
Lin9, a subunit of the mammalian DREAM complex, is essential for embryonic development, for survival of adult mice, and for tumor suppression. |
ARF-induced downregulation of Mip130/LIN-9 protein levels mediates a positive feedback that leads to increased expression of p16Ink4a and p19Arf. |
