Brap cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Brap
Entrez Gene ID	72399
Full Name	BRCA1 associated protein
Synonyms	3010002G07Rik,BRAP2,IMP
General protein information	Preferred Names BRCA1 associated protein Names BRCA1-associated protein RING-type E3 ubiquitin transferase BRAP2 brap impedes mitogenic signal propagation
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 F

mRNA and Protein(s)

mRNA	Protein	Name
XM_006530469.1	XP_006530532.1	BRCA1-associated protein isoform X1
NM_028227.3	NP_082503.2	BRCA1-associated protein isoform 1
NM_001289544.1	NP_001276473.1	BRCA1-associated protein isoform 3
XM_030254829.1	XP_030110689.1	BRCA1-associated protein isoform X1
NM_001289543.1	NP_001276472.1	BRCA1-associated protein isoform 2

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	BRAP	NP_001073229.1
Danio rerio (zebrafish)	brap	NP_001002466.1
	BRAP	XP_001102727.2
Mus musculus (house mouse)	Brap	NP_082503.2
Rattus norvegicus (Norway rat)	Brap	NP_001258123.1
Kluyveromyces lactis	KLLA0F25740g	XP_456226.1
Eremothecium gossypii	AGOS_AGL079C	NP_986587.1
Magnaporthe oryzae (rice blast fungus)	MGG_11239	XP_003714200.1
Bos taurus (cattle)	BRAP	NP_001092478.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002128	XP_003436037.1
Schizosaccharomyces pombe (fission yeast)	SPAC16E8.13	NP_594226.1
Xenopus tropicalis (tropical clawed frog)	brap	XP_002937733.1
Pan troglodytes (chimpanzee)	BRAP	NP_001233481.1
Neurospora crassa	NCU11215	XP_001728437.1
Homo sapiens (human)	BRAP	NP_006759.3
Canis lupus familiaris (dog)	BRAP	XP_543397.3
Drosophila melanogaster (fruit fly)	CG5555	NP_650789.1
Caenorhabditis elegans (roundworm)	EEED8.16	NP_495016.1
Saccharomyces cerevisiae (baker's yeast)	ETP1	NP_011853.1
Arabidopsis thaliana (thale cress)	BRIZ2	NP_001031419.1
Oryza sativa (rice)	Os02g0182900	NP_001046102.1

Gene Ontology
Bibliography

Related articles in PubMed

Loss of Brap Results in Premature G1/S Phase Transition and Impeded Neural Progenitor Differentiation.
Lanctot AA, Guo Y, Le Y, Edens BM, Nowakowski RS, Feng Y
Cell reports20(5)1148-1160(2017 Aug)

Interactome of the negative regulator of nuclear import BRCA1-binding protein 2.
Fatima S, Wagstaff KM, Loveland KL, Jans DA
Scientific reports59459(2015 Mar)

The BRCA1-binding protein BRAP2 can act as a cytoplasmic retention factor for nuclear and nuclear envelope-localizing testicular proteins.
Davies RG, Wagstaff KM, McLaughlin EA, Loveland KL, Jans DA
Biochimica et biophysica acta1833(12)3436-3444(2013 Dec)

Spatially dependent dynamic MAPK modulation by the Nde1-Lis1-Brap complex patterns mammalian CNS.
Lanctot AA, Peng CY, Pawlisz AS, Joksimovic M, Feng Y
Developmental cell25(3)241-55(2013 May)

p21 Ras/impedes mitogenic signal propagation regulates cytokine production and migration in CD4 T cells.
Czyzyk J, Chen HC, Bottomly K, Flavell RA
The Journal of biological chemistry283(34)23004-15(2008 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Together the results indicate for the first time that BRAP2 may play an important NRNI role in germ cells of the testis, with an additional, scaffold/structural role in mature spermatozoa.
Title: Interactome of the negative regulator of nuclear import BRCA1-binding protein 2.

ectopic expression of BRAP2 inhibits nuclear localization of HMG20A and NuMA1, and prevents nuclear envelope accumulation of SYNE2.
Title: The BRCA1-binding protein BRAP2 can act as a cytoplasmic retention factor for nuclear and nuclear envelope-localizing testicular proteins.

A molecular complex composed of Nde1, Lis1, and Brap regulates the dynamic MAPK signaling threshold in a spatially dependent fashion.
Title: Spatially dependent dynamic MAPK modulation by the Nde1-Lis1-Brap complex patterns mammalian CNS.

p21 Ras/Imp regulate cytokine production and migration in CD4 T cells
Title: p21 Ras/impedes mitogenic signal propagation regulates cytokine production and migration in CD4 T cells.

The following Brap gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Brap cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu14113	NM_001289543.1 Latest version!	Mus musculus BRCA1 associated protein (Brap), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu23124	NM_028227.3 Latest version!	Mus musculus BRCA1 associated protein (Brap), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu23153	NM_001289544.1 Latest version!	Mus musculus BRCA1 associated protein (Brap), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu36008	XM_006530469.1 Latest version!	Mus musculus BRCA1 associated protein (Brap), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu36008	XM_030254829.1 Latest version!	Mus musculus BRCA1 associated protein (Brap), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Brap ( NM_001289543.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001276472.1 Mus musculus BRCA1 associated protein (Brap), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu14113
Clone ID Related Accession (Same CDS sequence)	NM_001289543.1
Accession Version	NM_001289543.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1332bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	BRCA1-associated protein isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY739796.1, AK143718.1, BC062932.1 and AC116104.7. On Jan 23, 2014 this sequence version replaced XM_006530470.1. Transcript Variant: This variant (2) lacks an exon in the 5' coding region and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK143718.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849382 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCTCCC TAAAAGAAGA CGTTCGGCGC AGTGCCATGC TGTGTGTCCT CACCGTCCCT 
GCCACCATGA CCAGTCATGA CCTTATGAAG TTTGTTGCCC CATTCAATGA TGTAATTGAA 
CAAATGAAAA TCATCAGGGA CTCTACTCCG AATCAGTACA TGGTACTGAT CAAGTTCAGT 
GCGCAGGCTG ATGCAGACAG TTTCTACATG GCGTGCAATG GCCGCCAGTT CAACTCAATC 
GAAGATGACG TCTGCCAGCT GGTCTATGTG GAAAGGGCTG AAGTGCTGAA ATCTGAAGAT 
GGCGCCAGCC TCCCCGTGAT GGACCTGACG GAGCTGCCCA AGTGCACTGT GTGTCTGGAG 
CGGATGGACG AGTCTGTGAA TGGCATCCTC ACCACCCTCT GCAACCACAG CTTCCATAGT 
CAGTGTCTGC AGCGGTGGGA TGACACCACG TGTCCTGTGT GCCGATACTG TCAAACGCCA 
GAGCCAGTGG AAGAAAACAA ATGTTTTGAG TGTGGTGTCC AGGAAAACCT CTGGATTTGT 
TTAATATGCG GCCACATAGG CTGTGGGCGG TACGTGAGTC GGCATGCTTA CAAGCACTTT 
GAGGAGACCC AGCACACATA CGCCATGCAG CTCACCAACC ATCGAGTCTG GGACTATGCT 
GGAGATAATT ATGTCCATCG ACTGGTTGCA AGCAAGACGG ATGGAAAGAT CGTTCAGTAC 
GAGTGTGAGG GCGACACCTG CCAGGAAGAG AAGATAGATG CCTTACAGTT AGAGTACTCG 
TACCTGTTGA CAAGCCAGCT GGAATCGCAG CGGATATACT GGGAGAACAA AATCGTGCGC 
ATAGAGAAGG ACACGGCAGA GGAGATTAAC AACATGAAGA CCAAGTTTAA AGAGACCATC 
GAGAAGTGTG ACAGCCTGGA GCTCAGGCTC AGTGACCTCC TGAAGGAGAA GCAGTCTGTG 
GAAAGGAAGT GTACCCAGCT GAACACCAGA GTGGCCAAGC TCAGCACGGA GCTGCAGGAG 
GAGCAGGAGC TGAACAAGTG TCTGCGCGCC AACCAGCTGG TGCTGCAGAA CCAGCTCAAG 
GAGGAGGAGA AGCTGCTGAA GGAGACCTGT GCCCAGAAAG ACCTGCAGAT CACCGAGATC 
CAGGAGCAGC TGCGCGATGT CATGTTCTAC CTGGAGACAC AGCAGCAGAT CAGCCACCTG 
CCTGCGGAGA CGAGGCAGGA GATCCAGGAA GGCCAGATCA ACATCGCCAT GGCCTCAGCG 
CCCAACCCAC CCTCTTCCGG GGCCGGTGGG AAGCTGCAGT CCAGAAAGGG CCGCAGCAAG 
AGGGGCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001276472.1
CDS	453..1784
Translation	MTSLKEDVRRSAMLCVLTVPATMTSHDLMKFVAPFNDVIEQMKIIRDSTPNQYMVLIKFSAQADADSFYMACNGRQFNSIEDDVCQLVYVERAEVLKSEDGASLPVMDLTELPKCTVCLERMDESVNGILTTLCNHSFHSQCLQRWDDTTCPVCRYCQTPEPVEENKCFECGVQENLWICLICGHIGCGRYVSRHAYKHFEETQHTYAMQLTNHRVWDYAGDNYVHRLVASKTDGKIVQYECEGDTCQEEKIDALQLEYSYLLTSQLESQRIYWENKIVRIEKDTAEEINNMKTKFKETIEKCDSLELRLSDLLKEKQSVERKCTQLNTRVAKLSTELQEEQELNKCLRANQLVLQNQLKEEEKLLKETCAQKDLQITEIQEQLRDVMFYLETQQQISHLPAETRQEIQEGQINIAMASAPNPPSSGAGGKLQSRKGRSKRGK

Target ORF information:

RefSeq Version	NM_001289543.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus BRCA1 associated protein (Brap), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001289543.1

ORF Insert Sequence:

ATGACCTCCC TAAAAGAAGA CGTTCGGCGC AGTGCCATGC TGTGTGTCCT CACCGTCCCT 
GCCACCATGA CCAGTCATGA CCTTATGAAG TTTGTTGCCC CATTCAATGA TGTAATTGAA 
CAAATGAAAA TCATCAGGGA CTCTACTCCG AATCAGTACA TGGTACTGAT CAAGTTCAGT 
GCGCAGGCTG ATGCAGACAG TTTCTACATG GCGTGCAATG GCCGCCAGTT CAACTCAATC 
GAAGATGACG TCTGCCAGCT GGTCTATGTG GAAAGGGCTG AAGTGCTGAA ATCTGAAGAT 
GGCGCCAGCC TCCCCGTGAT GGACCTGACG GAGCTGCCCA AGTGCACTGT GTGTCTGGAG 
CGGATGGACG AGTCTGTGAA TGGCATCCTC ACCACCCTCT GCAACCACAG CTTCCATAGT 
CAGTGTCTGC AGCGGTGGGA TGACACCACG TGTCCTGTGT GCCGATACTG TCAAACGCCA 
GAGCCAGTGG AAGAAAACAA ATGTTTTGAG TGTGGTGTCC AGGAAAACCT CTGGATTTGT 
TTAATATGCG GCCACATAGG CTGTGGGCGG TACGTGAGTC GGCATGCTTA CAAGCACTTT 
GAGGAGACCC AGCACACATA CGCCATGCAG CTCACCAACC ATCGAGTCTG GGACTATGCT 
GGAGATAATT ATGTCCATCG ACTGGTTGCA AGCAAGACGG ATGGAAAGAT CGTTCAGTAC 
GAGTGTGAGG GCGACACCTG CCAGGAAGAG AAGATAGATG CCTTACAGTT AGAGTACTCG 
TACCTGTTGA CAAGCCAGCT GGAATCGCAG CGGATATACT GGGAGAACAA AATCGTGCGC 
ATAGAGAAGG ACACGGCAGA GGAGATTAAC AACATGAAGA CCAAGTTTAA AGAGACCATC 
GAGAAGTGTG ACAGCCTGGA GCTCAGGCTC AGTGACCTCC TGAAGGAGAA GCAGTCTGTG 
GAAAGGAAGT GTACCCAGCT GAACACCAGA GTGGCCAAGC TCAGCACGGA GCTGCAGGAG 
GAGCAGGAGC TGAACAAGTG TCTGCGCGCC AACCAGCTGG TGCTGCAGAA CCAGCTCAAG 
GAGGAGGAGA AGCTGCTGAA GGAGACCTGT GCCCAGAAAG ACCTGCAGAT CACCGAGATC 
CAGGAGCAGC TGCGCGATGT CATGTTCTAC CTGGAGACAC AGCAGCAGAT CAGCCACCTG 
CCTGCGGAGA CGAGGCAGGA GATCCAGGAA GGCCAGATCA ACATCGCCAT GGCCTCAGCG 
CCCAACCCAC CCTCTTCCGG GGCCGGTGGG AAGCTGCAGT CCAGAAAGGG CCGCAGCAAG 
AGGGGCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brap ( NM_028227.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_082503.2 Mus musculus BRCA1 associated protein (Brap), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu23124
Clone ID Related Accession (Same CDS sequence)	NM_028227.3
Accession Version	NM_028227.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1776bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	BRCA1-associated protein isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY739796.1, BC062932.1 and AC116104.7. On Jan 23, 2014 this sequence version replaced NM_028227.2. Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC062932.1, SRR1660811.72057.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTGTGT CACTGGTGGT CATCCGACTG GAGCTCGCGG GTCACTCGCC GGTCCCCACC 
GACTTCGGCT TCAGCGCCGC CGCTGGAGAG ATGTCTGATG AGGAGATCAA AAAGAAGACA 
TTGGCTTCCG CTGTCGCCTG TCTAGAAGGG AAGTCAGCCG GGGAGAAAGC AGCCATCATC 
CACCAGCACC TTGGCCGTCG GGAAATGACG GATGTGATTA TTGAGACCAT GAAGGCCAGA 
GCAGATGAAG TGAGAGACAC AGTGGAAGAA AAGAAGCCTT CAGCAGCCCC TGTCTCTGCA 
CAGAGAAGTA GAGAACAGAG TGAGTCTGTG AACACAGCCC CAGAGTCTCC ATCCAAACAG 
CTCCCAGACC AGATTTCCTT CTTCAGTGGC AACCCTTCTG TTGAAATAGT CCACGGCATT 
ATGCACCTGT ACAAGACAAA TAAGATGACC TCCCTAAAAG AAGACGTTCG GCGCAGTGCC 
ATGCTGTGTG TCCTCACCGT CCCTGCCACC ATGACCAGTC ATGACCTTAT GAAGTTTGTT 
GCCCCATTCA ATGATGTAAT TGAACAAATG AAAATCATCA GGGACTCTAC TCCGAATCAG 
TACATGGTAC TGATCAAGTT CAGTGCGCAG GCTGATGCAG ACAGTTTCTA CATGGCGTGC 
AATGGCCGCC AGTTCAACTC AATCGAAGAT GACGTCTGCC AGCTGGTCTA TGTGGAAAGG 
GCTGAAGTGC TGAAATCTGA AGATGGCGCC AGCCTCCCCG TGATGGACCT GACGGAGCTG 
CCCAAGTGCA CTGTGTGTCT GGAGCGGATG GACGAGTCTG TGAATGGCAT CCTCACCACC 
CTCTGCAACC ACAGCTTCCA TAGTCAGTGT CTGCAGCGGT GGGATGACAC CACGTGTCCT 
GTGTGCCGAT ACTGTCAAAC GCCAGAGCCA GTGGAAGAAA ACAAATGTTT TGAGTGTGGT 
GTCCAGGAAA ACCTCTGGAT TTGTTTAATA TGCGGCCACA TAGGCTGTGG GCGGTACGTG 
AGTCGGCATG CTTACAAGCA CTTTGAGGAG ACCCAGCACA CATACGCCAT GCAGCTCACC 
AACCATCGAG TCTGGGACTA TGCTGGAGAT AATTATGTCC ATCGACTGGT TGCAAGCAAG 
ACGGATGGAA AGATCGTTCA GTACGAGTGT GAGGGCGACA CCTGCCAGGA AGAGAAGATA 
GATGCCTTAC AGTTAGAGTA CTCGTACCTG TTGACAAGCC AGCTGGAATC GCAGCGGATA 
TACTGGGAGA ACAAAATCGT GCGCATAGAG AAGGACACGG CAGAGGAGAT TAACAACATG 
AAGACCAAGT TTAAAGAGAC CATCGAGAAG TGTGACAGCC TGGAGCTCAG GCTCAGTGAC 
CTCCTGAAGG AGAAGCAGTC TGTGGAAAGG AAGTGTACCC AGCTGAACAC CAGAGTGGCC 
AAGCTCAGCA CGGAGCTGCA GGAGGAGCAG GAGCTGAACA AGTGTCTGCG CGCCAACCAG 
CTGGTGCTGC AGAACCAGCT CAAGGAGGAG GAGAAGCTGC TGAAGGAGAC CTGTGCCCAG 
AAAGACCTGC AGATCACCGA GATCCAGGAG CAGCTGCGCG ATGTCATGTT CTACCTGGAG 
ACACAGCAGC AGATCAGCCA CCTGCCTGCG GAGACGAGGC AGGAGATCCA GGAAGGCCAG 
ATCAACATCG CCATGGCCTC AGCGCCCAAC CCACCCTCTT CCGGGGCCGG TGGGAAGCTG 
CAGTCCAGAA AGGGCCGCAG CAAGAGGGGC AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_082503.2
CDS	205..1980
Translation	MSVSLVVIRLELAGHSPVPTDFGFSAAAGEMSDEEIKKKTLASAVACLEGKSAGEKAAIIHQHLGRREMTDVIIETMKARADEVRDTVEEKKPSAAPVSAQRSREQSESVNTAPESPSKQLPDQISFFSGNPSVEIVHGIMHLYKTNKMTSLKEDVRRSAMLCVLTVPATMTSHDLMKFVAPFNDVIEQMKIIRDSTPNQYMVLIKFSAQADADSFYMACNGRQFNSIEDDVCQLVYVERAEVLKSEDGASLPVMDLTELPKCTVCLERMDESVNGILTTLCNHSFHSQCLQRWDDTTCPVCRYCQTPEPVEENKCFECGVQENLWICLICGHIGCGRYVSRHAYKHFEETQHTYAMQLTNHRVWDYAGDNYVHRLVASKTDGKIVQYECEGDTCQEEKIDALQLEYSYLLTSQLESQRIYWENKIVRIEKDTAEEINNMKTKFKETIEKCDSLELRLSDLLKEKQSVERKCTQLNTRVAKLSTELQEEQELNKCLRANQLVLQNQLKEEEKLLKETCAQKDLQITEIQEQLRDVMFYLETQQQISHLPAETRQEIQEGQINIAMASAPNPPSSGAGGKLQSRKGRSKRGK

Target ORF information:

RefSeq Version	NM_028227.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus BRCA1 associated protein (Brap), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_028227.3

ORF Insert Sequence:

ATGAGTGTGT CACTGGTGGT CATCCGACTG GAGCTCGCGG GTCACTCGCC GGTCCCCACC 
GACTTCGGCT TCAGCGCCGC CGCTGGAGAG ATGTCTGATG AGGAGATCAA AAAGAAGACA 
TTGGCTTCCG CTGTCGCCTG TCTAGAAGGG AAGTCAGCCG GGGAGAAAGC AGCCATCATC 
CACCAGCACC TTGGCCGTCG GGAAATGACG GATGTGATTA TTGAGACCAT GAAGGCCAGA 
GCAGATGAAG TGAGAGACAC AGTGGAAGAA AAGAAGCCTT CAGCAGCCCC TGTCTCTGCA 
CAGAGAAGTA GAGAACAGAG TGAGTCTGTG AACACAGCCC CAGAGTCTCC ATCCAAACAG 
CTCCCAGACC AGATTTCCTT CTTCAGTGGC AACCCTTCTG TTGAAATAGT CCACGGCATT 
ATGCACCTGT ACAAGACAAA TAAGATGACC TCCCTAAAAG AAGACGTTCG GCGCAGTGCC 
ATGCTGTGTG TCCTCACCGT CCCTGCCACC ATGACCAGTC ATGACCTTAT GAAGTTTGTT 
GCCCCATTCA ATGATGTAAT TGAACAAATG AAAATCATCA GGGACTCTAC TCCGAATCAG 
TACATGGTAC TGATCAAGTT CAGTGCGCAG GCTGATGCAG ACAGTTTCTA CATGGCGTGC 
AATGGCCGCC AGTTCAACTC AATCGAAGAT GACGTCTGCC AGCTGGTCTA TGTGGAAAGG 
GCTGAAGTGC TGAAATCTGA AGATGGCGCC AGCCTCCCCG TGATGGACCT GACGGAGCTG 
CCCAAGTGCA CTGTGTGTCT GGAGCGGATG GACGAGTCTG TGAATGGCAT CCTCACCACC 
CTCTGCAACC ACAGCTTCCA TAGTCAGTGT CTGCAGCGGT GGGATGACAC CACGTGTCCT 
GTGTGCCGAT ACTGTCAAAC GCCAGAGCCA GTGGAAGAAA ACAAATGTTT TGAGTGTGGT 
GTCCAGGAAA ACCTCTGGAT TTGTTTAATA TGCGGCCACA TAGGCTGTGG GCGGTACGTG 
AGTCGGCATG CTTACAAGCA CTTTGAGGAG ACCCAGCACA CATACGCCAT GCAGCTCACC 
AACCATCGAG TCTGGGACTA TGCTGGAGAT AATTATGTCC ATCGACTGGT TGCAAGCAAG 
ACGGATGGAA AGATCGTTCA GTACGAGTGT GAGGGCGACA CCTGCCAGGA AGAGAAGATA 
GATGCCTTAC AGTTAGAGTA CTCGTACCTG TTGACAAGCC AGCTGGAATC GCAGCGGATA 
TACTGGGAGA ACAAAATCGT GCGCATAGAG AAGGACACGG CAGAGGAGAT TAACAACATG 
AAGACCAAGT TTAAAGAGAC CATCGAGAAG TGTGACAGCC TGGAGCTCAG GCTCAGTGAC 
CTCCTGAAGG AGAAGCAGTC TGTGGAAAGG AAGTGTACCC AGCTGAACAC CAGAGTGGCC 
AAGCTCAGCA CGGAGCTGCA GGAGGAGCAG GAGCTGAACA AGTGTCTGCG CGCCAACCAG 
CTGGTGCTGC AGAACCAGCT CAAGGAGGAG GAGAAGCTGC TGAAGGAGAC CTGTGCCCAG 
AAAGACCTGC AGATCACCGA GATCCAGGAG CAGCTGCGCG ATGTCATGTT CTACCTGGAG 
ACACAGCAGC AGATCAGCCA CCTGCCTGCG GAGACGAGGC AGGAGATCCA GGAAGGCCAG 
ATCAACATCG CCATGGCCTC AGCGCCCAAC CCACCCTCTT CCGGGGCCGG TGGGAAGCTG 
CAGTCCAGAA AGGGCCGCAG CAAGAGGGGC AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brap ( NM_001289544.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001276473.1 Mus musculus BRCA1 associated protein (Brap), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu23153
Clone ID Related Accession (Same CDS sequence)	NM_001289544.1
Accession Version	NM_001289544.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1686bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	BRCA1-associated protein isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC116104.7. Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (3) has a shorter N-terminus than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AF321921.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849384 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTGATG AGGAGATCAA AAAGAAGACA TTGGCTTCCG CTGTCGCCTG TCTAGAAGGG 
AAGTCAGCCG GGGAGAAAGC AGCCATCATC CACCAGCACC TTGGCCGTCG GGAAATGACG 
GATGTGATTA TTGAGACCAT GAAGGCCAGA GCAGATGAAG TGAGAGACAC AGTGGAAGAA 
AAGAAGCCTT CAGCAGCCCC TGTCTCTGCA CAGAGAAGTA GAGAACAGAG TGAGTCTGTG 
AACACAGCCC CAGAGTCTCC ATCCAAACAG CTCCCAGACC AGATTTCCTT CTTCAGTGGC 
AACCCTTCTG TTGAAATAGT CCACGGCATT ATGCACCTGT ACAAGACAAA TAAGATGACC 
TCCCTAAAAG AAGACGTTCG GCGCAGTGCC ATGCTGTGTG TCCTCACCGT CCCTGCCACC 
ATGACCAGTC ATGACCTTAT GAAGTTTGTT GCCCCATTCA ATGATGTAAT TGAACAAATG 
AAAATCATCA GGGACTCTAC TCCGAATCAG TACATGGTAC TGATCAAGTT CAGTGCGCAG 
GCTGATGCAG ACAGTTTCTA CATGGCGTGC AATGGCCGCC AGTTCAACTC AATCGAAGAT 
GACGTCTGCC AGCTGGTCTA TGTGGAAAGG GCTGAAGTGC TGAAATCTGA AGATGGCGCC 
AGCCTCCCCG TGATGGACCT GACGGAGCTG CCCAAGTGCA CTGTGTGTCT GGAGCGGATG 
GACGAGTCTG TGAATGGCAT CCTCACCACC CTCTGCAACC ACAGCTTCCA TAGTCAGTGT 
CTGCAGCGGT GGGATGACAC CACGTGTCCT GTGTGCCGAT ACTGTCAAAC GCCAGAGCCA 
GTGGAAGAAA ACAAATGTTT TGAGTGTGGT GTCCAGGAAA ACCTCTGGAT TTGTTTAATA 
TGCGGCCACA TAGGCTGTGG GCGGTACGTG AGTCGGCATG CTTACAAGCA CTTTGAGGAG 
ACCCAGCACA CATACGCCAT GCAGCTCACC AACCATCGAG TCTGGGACTA TGCTGGAGAT 
AATTATGTCC ATCGACTGGT TGCAAGCAAG ACGGATGGAA AGATCGTTCA GTACGAGTGT 
GAGGGCGACA CCTGCCAGGA AGAGAAGATA GATGCCTTAC AGTTAGAGTA CTCGTACCTG 
TTGACAAGCC AGCTGGAATC GCAGCGGATA TACTGGGAGA ACAAAATCGT GCGCATAGAG 
AAGGACACGG CAGAGGAGAT TAACAACATG AAGACCAAGT TTAAAGAGAC CATCGAGAAG 
TGTGACAGCC TGGAGCTCAG GCTCAGTGAC CTCCTGAAGG AGAAGCAGTC TGTGGAAAGG 
AAGTGTACCC AGCTGAACAC CAGAGTGGCC AAGCTCAGCA CGGAGCTGCA GGAGGAGCAG 
GAGCTGAACA AGTGTCTGCG CGCCAACCAG CTGGTGCTGC AGAACCAGCT CAAGGAGGAG 
GAGAAGCTGC TGAAGGAGAC CTGTGCCCAG AAAGACCTGC AGATCACCGA GATCCAGGAG 
CAGCTGCGCG ATGTCATGTT CTACCTGGAG ACACAGCAGC AGATCAGCCA CCTGCCTGCG 
GAGACGAGGC AGGAGATCCA GGAAGGCCAG ATCAACATCG CCATGGCCTC AGCGCCCAAC 
CCACCCTCTT CCGGGGCCGG TGGGAAGCTG CAGTCCAGAA AGGGCCGCAG CAAGAGGGGC 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001276473.1
CDS	423..2108
Translation	MSDEEIKKKTLASAVACLEGKSAGEKAAIIHQHLGRREMTDVIIETMKARADEVRDTVEEKKPSAAPVSAQRSREQSESVNTAPESPSKQLPDQISFFSGNPSVEIVHGIMHLYKTNKMTSLKEDVRRSAMLCVLTVPATMTSHDLMKFVAPFNDVIEQMKIIRDSTPNQYMVLIKFSAQADADSFYMACNGRQFNSIEDDVCQLVYVERAEVLKSEDGASLPVMDLTELPKCTVCLERMDESVNGILTTLCNHSFHSQCLQRWDDTTCPVCRYCQTPEPVEENKCFECGVQENLWICLICGHIGCGRYVSRHAYKHFEETQHTYAMQLTNHRVWDYAGDNYVHRLVASKTDGKIVQYECEGDTCQEEKIDALQLEYSYLLTSQLESQRIYWENKIVRIEKDTAEEINNMKTKFKETIEKCDSLELRLSDLLKEKQSVERKCTQLNTRVAKLSTELQEEQELNKCLRANQLVLQNQLKEEEKLLKETCAQKDLQITEIQEQLRDVMFYLETQQQISHLPAETRQEIQEGQINIAMASAPNPPSSGAGGKLQSRKGRSKRGK

Target ORF information:

RefSeq Version	NM_001289544.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus BRCA1 associated protein (Brap), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001289544.1

ORF Insert Sequence:

ATGTCTGATG AGGAGATCAA AAAGAAGACA TTGGCTTCCG CTGTCGCCTG TCTAGAAGGG 
AAGTCAGCCG GGGAGAAAGC AGCCATCATC CACCAGCACC TTGGCCGTCG GGAAATGACG 
GATGTGATTA TTGAGACCAT GAAGGCCAGA GCAGATGAAG TGAGAGACAC AGTGGAAGAA 
AAGAAGCCTT CAGCAGCCCC TGTCTCTGCA CAGAGAAGTA GAGAACAGAG TGAGTCTGTG 
AACACAGCCC CAGAGTCTCC ATCCAAACAG CTCCCAGACC AGATTTCCTT CTTCAGTGGC 
AACCCTTCTG TTGAAATAGT CCACGGCATT ATGCACCTGT ACAAGACAAA TAAGATGACC 
TCCCTAAAAG AAGACGTTCG GCGCAGTGCC ATGCTGTGTG TCCTCACCGT CCCTGCCACC 
ATGACCAGTC ATGACCTTAT GAAGTTTGTT GCCCCATTCA ATGATGTAAT TGAACAAATG 
AAAATCATCA GGGACTCTAC TCCGAATCAG TACATGGTAC TGATCAAGTT CAGTGCGCAG 
GCTGATGCAG ACAGTTTCTA CATGGCGTGC AATGGCCGCC AGTTCAACTC AATCGAAGAT 
GACGTCTGCC AGCTGGTCTA TGTGGAAAGG GCTGAAGTGC TGAAATCTGA AGATGGCGCC 
AGCCTCCCCG TGATGGACCT GACGGAGCTG CCCAAGTGCA CTGTGTGTCT GGAGCGGATG 
GACGAGTCTG TGAATGGCAT CCTCACCACC CTCTGCAACC ACAGCTTCCA TAGTCAGTGT 
CTGCAGCGGT GGGATGACAC CACGTGTCCT GTGTGCCGAT ACTGTCAAAC GCCAGAGCCA 
GTGGAAGAAA ACAAATGTTT TGAGTGTGGT GTCCAGGAAA ACCTCTGGAT TTGTTTAATA 
TGCGGCCACA TAGGCTGTGG GCGGTACGTG AGTCGGCATG CTTACAAGCA CTTTGAGGAG 
ACCCAGCACA CATACGCCAT GCAGCTCACC AACCATCGAG TCTGGGACTA TGCTGGAGAT 
AATTATGTCC ATCGACTGGT TGCAAGCAAG ACGGATGGAA AGATCGTTCA GTACGAGTGT 
GAGGGCGACA CCTGCCAGGA AGAGAAGATA GATGCCTTAC AGTTAGAGTA CTCGTACCTG 
TTGACAAGCC AGCTGGAATC GCAGCGGATA TACTGGGAGA ACAAAATCGT GCGCATAGAG 
AAGGACACGG CAGAGGAGAT TAACAACATG AAGACCAAGT TTAAAGAGAC CATCGAGAAG 
TGTGACAGCC TGGAGCTCAG GCTCAGTGAC CTCCTGAAGG AGAAGCAGTC TGTGGAAAGG 
AAGTGTACCC AGCTGAACAC CAGAGTGGCC AAGCTCAGCA CGGAGCTGCA GGAGGAGCAG 
GAGCTGAACA AGTGTCTGCG CGCCAACCAG CTGGTGCTGC AGAACCAGCT CAAGGAGGAG 
GAGAAGCTGC TGAAGGAGAC CTGTGCCCAG AAAGACCTGC AGATCACCGA GATCCAGGAG 
CAGCTGCGCG ATGTCATGTT CTACCTGGAG ACACAGCAGC AGATCAGCCA CCTGCCTGCG 
GAGACGAGGC AGGAGATCCA GGAAGGCCAG ATCAACATCG CCATGGCCTC AGCGCCCAAC 
CCACCCTCTT CCGGGGCCGG TGGGAAGCTG CAGTCCAGAA AGGGCCGCAG CAAGAGGGGC 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brap ( XM_006530469.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530532.1 Mus musculus BRCA1 associated protein (Brap), transcript variant X1, mRNA.

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CloneID	OMu36008
Clone ID Related Accession (Same CDS sequence)	XM_006530469.1 , XM_030254829.1
Accession Version	XM_006530469.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1356bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	BRCA1-associated protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACCTGT ACAAGACAAA TAAGATGACC TCCCTAAAAG AAGACGTTCG GCGCAGTGCC 
ATGCTGTGTG TCCTCACCGT CCCTGCCACC ATGACCAGTC ATGACCTTAT GAAGTTTGTT 
GCCCCATTCA ATGATGTAAT TGAACAAATG AAAATCATCA GGGACTCTAC TCCGAATCAG 
TACATGGTAC TGATCAAGTT CAGTGCGCAG GCTGATGCAG ACAGTTTCTA CATGGCGTGC 
AATGGCCGCC AGTTCAACTC AATCGAAGAT GACGTCTGCC AGCTGGTCTA TGTGGAAAGG 
GCTGAAGTGC TGAAATCTGA AGATGGCGCC AGCCTCCCCG TGATGGACCT GACGGAGCTG 
CCCAAGTGCA CTGTGTGTCT GGAGCGGATG GACGAGTCTG TGAATGGCAT CCTCACCACC 
CTCTGCAACC ACAGCTTCCA TAGTCAGTGT CTGCAGCGGT GGGATGACAC CACGTGTCCT 
GTGTGCCGAT ACTGTCAAAC GCCAGAGCCA GTGGAAGAAA ACAAATGTTT TGAGTGTGGT 
GTCCAGGAAA ACCTCTGGAT TTGTTTAATA TGCGGCCACA TAGGCTGTGG GCGGTACGTG 
AGTCGGCATG CTTACAAGCA CTTTGAGGAG ACCCAGCACA CATACGCCAT GCAGCTCACC 
AACCATCGAG TCTGGGACTA TGCTGGAGAT AATTATGTCC ATCGACTGGT TGCAAGCAAG 
ACGGATGGAA AGATCGTTCA GTACGAGTGT GAGGGCGACA CCTGCCAGGA AGAGAAGATA 
GATGCCTTAC AGTTAGAGTA CTCGTACCTG TTGACAAGCC AGCTGGAATC GCAGCGGATA 
TACTGGGAGA ACAAAATCGT GCGCATAGAG AAGGACACGG CAGAGGAGAT TAACAACATG 
AAGACCAAGT TTAAAGAGAC CATCGAGAAG TGTGACAGCC TGGAGCTCAG GCTCAGTGAC 
CTCCTGAAGG AGAAGCAGTC TGTGGAAAGG AAGTGTACCC AGCTGAACAC CAGAGTGGCC 
AAGCTCAGCA CGGAGCTGCA GGAGGAGCAG GAGCTGAACA AGTGTCTGCG CGCCAACCAG 
CTGGTGCTGC AGAACCAGCT CAAGGAGGAG GAGAAGCTGC TGAAGGAGAC CTGTGCCCAG 
AAAGACCTGC AGATCACCGA GATCCAGGAG CAGCTGCGCG ATGTCATGTT CTACCTGGAG 
ACACAGCAGC AGATCAGCCA CCTGCCTGCG GAGACGAGGC AGGAGATCCA GGAAGGCCAG 
ATCAACATCG CCATGGCCTC AGCGCCCAAC CCACCCTCTT CCGGGGCCGG TGGGAAGCTG 
CAGTCCAGAA AGGGCCGCAG CAAGAGGGGC AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530532.1
CDS	352..1707
Translation	MHLYKTNKMTSLKEDVRRSAMLCVLTVPATMTSHDLMKFVAPFNDVIEQMKIIRDSTPNQYMVLIKFSAQADADSFYMACNGRQFNSIEDDVCQLVYVERAEVLKSEDGASLPVMDLTELPKCTVCLERMDESVNGILTTLCNHSFHSQCLQRWDDTTCPVCRYCQTPEPVEENKCFECGVQENLWICLICGHIGCGRYVSRHAYKHFEETQHTYAMQLTNHRVWDYAGDNYVHRLVASKTDGKIVQYECEGDTCQEEKIDALQLEYSYLLTSQLESQRIYWENKIVRIEKDTAEEINNMKTKFKETIEKCDSLELRLSDLLKEKQSVERKCTQLNTRVAKLSTELQEEQELNKCLRANQLVLQNQLKEEEKLLKETCAQKDLQITEIQEQLRDVMFYLETQQQISHLPAETRQEIQEGQINIAMASAPNPPSSGAGGKLQSRKGRSKRGK

Target ORF information:

RefSeq Version	XM_006530469.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus BRCA1 associated protein (Brap), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530469.1

ORF Insert Sequence:

ATGCACCTGT ACAAGACAAA TAAGATGACC TCCCTAAAAG AAGACGTTCG GCGCAGTGCC 
ATGCTGTGTG TCCTCACCGT CCCTGCCACC ATGACCAGTC ATGACCTTAT GAAGTTTGTT 
GCCCCATTCA ATGATGTAAT TGAACAAATG AAAATCATCA GGGACTCTAC TCCGAATCAG 
TACATGGTAC TGATCAAGTT CAGTGCGCAG GCTGATGCAG ACAGTTTCTA CATGGCGTGC 
AATGGCCGCC AGTTCAACTC AATCGAAGAT GACGTCTGCC AGCTGGTCTA TGTGGAAAGG 
GCTGAAGTGC TGAAATCTGA AGATGGCGCC AGCCTCCCCG TGATGGACCT GACGGAGCTG 
CCCAAGTGCA CTGTGTGTCT GGAGCGGATG GACGAGTCTG TGAATGGCAT CCTCACCACC 
CTCTGCAACC ACAGCTTCCA TAGTCAGTGT CTGCAGCGGT GGGATGACAC CACGTGTCCT 
GTGTGCCGAT ACTGTCAAAC GCCAGAGCCA GTGGAAGAAA ACAAATGTTT TGAGTGTGGT 
GTCCAGGAAA ACCTCTGGAT TTGTTTAATA TGCGGCCACA TAGGCTGTGG GCGGTACGTG 
AGTCGGCATG CTTACAAGCA CTTTGAGGAG ACCCAGCACA CATACGCCAT GCAGCTCACC 
AACCATCGAG TCTGGGACTA TGCTGGAGAT AATTATGTCC ATCGACTGGT TGCAAGCAAG 
ACGGATGGAA AGATCGTTCA GTACGAGTGT GAGGGCGACA CCTGCCAGGA AGAGAAGATA 
GATGCCTTAC AGTTAGAGTA CTCGTACCTG TTGACAAGCC AGCTGGAATC GCAGCGGATA 
TACTGGGAGA ACAAAATCGT GCGCATAGAG AAGGACACGG CAGAGGAGAT TAACAACATG 
AAGACCAAGT TTAAAGAGAC CATCGAGAAG TGTGACAGCC TGGAGCTCAG GCTCAGTGAC 
CTCCTGAAGG AGAAGCAGTC TGTGGAAAGG AAGTGTACCC AGCTGAACAC CAGAGTGGCC 
AAGCTCAGCA CGGAGCTGCA GGAGGAGCAG GAGCTGAACA AGTGTCTGCG CGCCAACCAG 
CTGGTGCTGC AGAACCAGCT CAAGGAGGAG GAGAAGCTGC TGAAGGAGAC CTGTGCCCAG 
AAAGACCTGC AGATCACCGA GATCCAGGAG CAGCTGCGCG ATGTCATGTT CTACCTGGAG 
ACACAGCAGC AGATCAGCCA CCTGCCTGCG GAGACGAGGC AGGAGATCCA GGAAGGCCAG 
ATCAACATCG CCATGGCCTC AGCGCCCAAC CCACCCTCTT CCGGGGCCGG TGGGAAGCTG 
CAGTCCAGAA AGGGCCGCAG CAAGAGGGGC AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brap ( XM_030254829.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030110689.1 Mus musculus BRCA1 associated protein (Brap), transcript variant X1, mRNA.

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CloneID	OMu36008
Clone ID Related Accession (Same CDS sequence)	XM_006530469.1 , XM_030254829.1
Accession Version	XM_030254829.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1356bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	BRCA1-associated protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACCTGT ACAAGACAAA TAAGATGACC TCCCTAAAAG AAGACGTTCG GCGCAGTGCC 
ATGCTGTGTG TCCTCACCGT CCCTGCCACC ATGACCAGTC ATGACCTTAT GAAGTTTGTT 
GCCCCATTCA ATGATGTAAT TGAACAAATG AAAATCATCA GGGACTCTAC TCCGAATCAG 
TACATGGTAC TGATCAAGTT CAGTGCGCAG GCTGATGCAG ACAGTTTCTA CATGGCGTGC 
AATGGCCGCC AGTTCAACTC AATCGAAGAT GACGTCTGCC AGCTGGTCTA TGTGGAAAGG 
GCTGAAGTGC TGAAATCTGA AGATGGCGCC AGCCTCCCCG TGATGGACCT GACGGAGCTG 
CCCAAGTGCA CTGTGTGTCT GGAGCGGATG GACGAGTCTG TGAATGGCAT CCTCACCACC 
CTCTGCAACC ACAGCTTCCA TAGTCAGTGT CTGCAGCGGT GGGATGACAC CACGTGTCCT 
GTGTGCCGAT ACTGTCAAAC GCCAGAGCCA GTGGAAGAAA ACAAATGTTT TGAGTGTGGT 
GTCCAGGAAA ACCTCTGGAT TTGTTTAATA TGCGGCCACA TAGGCTGTGG GCGGTACGTG 
AGTCGGCATG CTTACAAGCA CTTTGAGGAG ACCCAGCACA CATACGCCAT GCAGCTCACC 
AACCATCGAG TCTGGGACTA TGCTGGAGAT AATTATGTCC ATCGACTGGT TGCAAGCAAG 
ACGGATGGAA AGATCGTTCA GTACGAGTGT GAGGGCGACA CCTGCCAGGA AGAGAAGATA 
GATGCCTTAC AGTTAGAGTA CTCGTACCTG TTGACAAGCC AGCTGGAATC GCAGCGGATA 
TACTGGGAGA ACAAAATCGT GCGCATAGAG AAGGACACGG CAGAGGAGAT TAACAACATG 
AAGACCAAGT TTAAAGAGAC CATCGAGAAG TGTGACAGCC TGGAGCTCAG GCTCAGTGAC 
CTCCTGAAGG AGAAGCAGTC TGTGGAAAGG AAGTGTACCC AGCTGAACAC CAGAGTGGCC 
AAGCTCAGCA CGGAGCTGCA GGAGGAGCAG GAGCTGAACA AGTGTCTGCG CGCCAACCAG 
CTGGTGCTGC AGAACCAGCT CAAGGAGGAG GAGAAGCTGC TGAAGGAGAC CTGTGCCCAG 
AAAGACCTGC AGATCACCGA GATCCAGGAG CAGCTGCGCG ATGTCATGTT CTACCTGGAG 
ACACAGCAGC AGATCAGCCA CCTGCCTGCG GAGACGAGGC AGGAGATCCA GGAAGGCCAG 
ATCAACATCG CCATGGCCTC AGCGCCCAAC CCACCCTCTT CCGGGGCCGG TGGGAAGCTG 
CAGTCCAGAA AGGGCCGCAG CAAGAGGGGC AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030110689.1
CDS	674..2029
Translation	MHLYKTNKMTSLKEDVRRSAMLCVLTVPATMTSHDLMKFVAPFNDVIEQMKIIRDSTPNQYMVLIKFSAQADADSFYMACNGRQFNSIEDDVCQLVYVERAEVLKSEDGASLPVMDLTELPKCTVCLERMDESVNGILTTLCNHSFHSQCLQRWDDTTCPVCRYCQTPEPVEENKCFECGVQENLWICLICGHIGCGRYVSRHAYKHFEETQHTYAMQLTNHRVWDYAGDNYVHRLVASKTDGKIVQYECEGDTCQEEKIDALQLEYSYLLTSQLESQRIYWENKIVRIEKDTAEEINNMKTKFKETIEKCDSLELRLSDLLKEKQSVERKCTQLNTRVAKLSTELQEEQELNKCLRANQLVLQNQLKEEEKLLKETCAQKDLQITEIQEQLRDVMFYLETQQQISHLPAETRQEIQEGQINIAMASAPNPPSSGAGGKLQSRKGRSKRGK

Target ORF information:

RefSeq Version	XM_030254829.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus BRCA1 associated protein (Brap), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030254829.1

ORF Insert Sequence:

ATGCACCTGT ACAAGACAAA TAAGATGACC TCCCTAAAAG AAGACGTTCG GCGCAGTGCC 
ATGCTGTGTG TCCTCACCGT CCCTGCCACC ATGACCAGTC ATGACCTTAT GAAGTTTGTT 
GCCCCATTCA ATGATGTAAT TGAACAAATG AAAATCATCA GGGACTCTAC TCCGAATCAG 
TACATGGTAC TGATCAAGTT CAGTGCGCAG GCTGATGCAG ACAGTTTCTA CATGGCGTGC 
AATGGCCGCC AGTTCAACTC AATCGAAGAT GACGTCTGCC AGCTGGTCTA TGTGGAAAGG 
GCTGAAGTGC TGAAATCTGA AGATGGCGCC AGCCTCCCCG TGATGGACCT GACGGAGCTG 
CCCAAGTGCA CTGTGTGTCT GGAGCGGATG GACGAGTCTG TGAATGGCAT CCTCACCACC 
CTCTGCAACC ACAGCTTCCA TAGTCAGTGT CTGCAGCGGT GGGATGACAC CACGTGTCCT 
GTGTGCCGAT ACTGTCAAAC GCCAGAGCCA GTGGAAGAAA ACAAATGTTT TGAGTGTGGT 
GTCCAGGAAA ACCTCTGGAT TTGTTTAATA TGCGGCCACA TAGGCTGTGG GCGGTACGTG 
AGTCGGCATG CTTACAAGCA CTTTGAGGAG ACCCAGCACA CATACGCCAT GCAGCTCACC 
AACCATCGAG TCTGGGACTA TGCTGGAGAT AATTATGTCC ATCGACTGGT TGCAAGCAAG 
ACGGATGGAA AGATCGTTCA GTACGAGTGT GAGGGCGACA CCTGCCAGGA AGAGAAGATA 
GATGCCTTAC AGTTAGAGTA CTCGTACCTG TTGACAAGCC AGCTGGAATC GCAGCGGATA 
TACTGGGAGA ACAAAATCGT GCGCATAGAG AAGGACACGG CAGAGGAGAT TAACAACATG 
AAGACCAAGT TTAAAGAGAC CATCGAGAAG TGTGACAGCC TGGAGCTCAG GCTCAGTGAC 
CTCCTGAAGG AGAAGCAGTC TGTGGAAAGG AAGTGTACCC AGCTGAACAC CAGAGTGGCC 
AAGCTCAGCA CGGAGCTGCA GGAGGAGCAG GAGCTGAACA AGTGTCTGCG CGCCAACCAG 
CTGGTGCTGC AGAACCAGCT CAAGGAGGAG GAGAAGCTGC TGAAGGAGAC CTGTGCCCAG 
AAAGACCTGC AGATCACCGA GATCCAGGAG CAGCTGCGCG ATGTCATGTT CTACCTGGAG 
ACACAGCAGC AGATCAGCCA CCTGCCTGCG GAGACGAGGC AGGAGATCCA GGAAGGCCAG 
ATCAACATCG CCATGGCCTC AGCGCCCAAC CCACCCTCTT CCGGGGCCGG TGGGAAGCTG 
CAGTCCAGAA AGGGCCGCAG CAAGAGGGGC AAGTGA