Shroom1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Shroom1
Entrez Gene ID	71774
Full Name	shroom family member 1
Synonyms	Apx,Shrm1
General protein information	Preferred Names shroom family member 1 Names protein Shroom1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 B1.3

mRNA and Protein(s)

mRNA	Protein	Name
XM_006534259.3	XP_006534322.1	protein Shroom1 isoform X1
NM_027917.3	NP_082193.2	protein Shroom1 isoform 1
XM_006534259.4	XP_006534322.1	protein Shroom1 isoform X1
NM_001290789.1	NP_001277718.1	protein Shroom1 isoform 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	SHROOM1	NP_597713.2
	LOC711299	XP_001100079.2
Bos taurus (cattle)	SHROOM1	NP_001095764.1
Pan troglodytes (chimpanzee)	SHROOM1	XP_527007.4
Rattus norvegicus (Norway rat)	Shroom1	XP_006246428.1
Canis lupus familiaris (dog)	SHROOM1	XP_005626585.1
Mus musculus (house mouse)	Shroom1	NP_082193.2

Gene Ontology
Bibliography

Related articles in PubMed

Structure of Shroom domain 2 reveals a three-segmented coiled-coil required for dimerization, Rock binding, and apical constriction.
Mohan S, Rizaldy R, Das D, Bauer RJ, Heroux A, Trakselis MA, Hildebrand JD, VanDemark AP
Molecular biology of the cell23(11)2131-42(2012 Jun)

The shroom family proteins play broad roles in the morphogenesis of thickened epithelial sheets.
Lee C, Le MP, Wallingford JB
Developmental dynamics : an official publication of the American Association of Anatomists238(6)1480-91(2009 Jun)

Shroom expression is attenuated in pulmonary arterial hypertension.
Sevilla-P?rez J, K?nigshoff M, Kwapiszewska G, Amarie OV, Seeger W, Weissmann N, Schermuly RT, Morty RE, Eickelberg O
The European respiratory journal32(4)871-80(2008 Oct)

Shroom2, a myosin-VIIa- and actin-binding protein, directly interacts with ZO-1 at tight junctions.
Etournay R, Zwaenepoel I, Perfettini I, Legrain P, Petit C, El-Amraoui A
Journal of cell science120(Pt 16)2838-50(2007 Aug)

Differential actin-dependent localization modulates the evolutionarily conserved activity of Shroom family proteins.
Dietz ML, Bernaciak TM, Vendetti F, Kielec JM, Hildebrand JD
The Journal of biological chemistry281(29)20542-54(2006 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The structure of Shrm domain 2 (SD2), which mediates the interaction with Rock and is required for Shrm function, is reported.
Title: Structure of Shroom domain 2 reveals a three-segmented coiled-coil required for dimerization, Rock binding, and apical constriction.

Shroom localises exclusively to pulmonary smooth muscle cells and is attenuated in pulmonary arterial hypertension
Title: Shroom expression is attenuated in pulmonary arterial hypertension.

The following Shroom1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Shroom1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu14383	NM_027917.3 Latest version!	Mus musculus shroom family member 1 (Shroom1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu17427	NM_001290789.1 Latest version!	Mus musculus shroom family member 1 (Shroom1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
OMu46955	XM_006534259.4 Latest version!	Mus musculus shroom family member 1 (Shroom1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OMu46955	XM_006534259.3	Mus musculus shroom family member 1 (Shroom1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Shroom1 ( NM_027917.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_082193.2 Mus musculus shroom family member 1 (Shroom1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu14383
Clone ID Related Accession (Same CDS sequence)	NM_027917.3
Accession Version	NM_027917.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2472bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Mus musculus(house mouse)
Product	protein Shroom1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL596095.18, AK134332.1 and AK035462.1. On Mar 20, 2014 this sequence version replaced NM_027917.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). CCDS Note: The coding region has been updated to include an additional internal exon that is better supported by transcripts from the reference genome strain, and is also supported by homology data. ##Evidence-Data-START## Transcript exon combination :: AK134332.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGAGGCCC TGGGTACTGG GAGAGACCGC ACCTCCCAGG CCTCAGCCAC TGAAAGCCTG 
GACTTGCGAC GGTTGTCCAC GCGCGCCGAC TCTGCCTACA GCTCTTTCTC CACGGCGTCT 
GGTGATCCTG AGACGCGCAC TCCGTCCCCT GGCACCGAGC GCCTCCCTTA CCTAGACTGG 
GACTACGTGC GAGTGGTTTG GGGCAGCCAA TCTCCTACCT CGAAGGACGC TGTCCTTTCA 
ACGACTCAGC GACCTGTGCA GGCAGTCGCT GGGCACAGTG ACCCACGGTC CCCAGAGGTC 
CAGGGAAGCC CGGGACCACT AAACAGACAA GACACCCCAC TGCTGTACGC GCTGGCCGCG 
GAGGCTGAAG CTACAGCTCA CACCGCAGAG CCGCCCAGCC CGCCAGCCTC GCGGGACGCC 
TACCGCCAGC GGTTGCAGGG TGCACAGCGA CGAGTGCTGC GGGAGACGTC CTTCCAGCGA 
AAGGAGTTTC GCATGAGCCT GCCGGGCCGC CTGCGTCCCG CAGTCCCCAC GCGACTTCCC 
ACGGCGCACG TGCGCTCCGC CTCCAGCAGC CAGGAGCTGG GAGAAGAGGA GCCGGCGCGC 
ACCGCTGTCC CAGCGCTAGC TGCTGCTGGC CGGGGGCGTC TCTCCAGCCA GCAGCGGCAG 
TGCTGCTTCT CTGAGCCAGG CAAATTACAT CGCGTAGGTT GGAGCGGTGG GCCCACTGGT 
GAGGACTTGA GAAAAGATTA TTCCACGCAG GAGTTGCAGC GTGGGATGCA CGCAAAGTCC 
AAAGGGCTGC TGGAGACTCA GTCCCTAAGC TCAACGGAGC TGAATTCCGG GCCAGCGGAT 
CTTGGCAATG CCCATAGACC TGCGGGTCGG AGTCAGAGTG TTTCAGGCGA GGTCATGGGT 
CCCTGCAAGG GTTCAGAAAG GACTGTGGCC ACTGTCCAGG CTGTTCCTCA AAGAGCAGAC 
ATCCGCAGAC CACTGCTTCA CACCAAGCTT TCCAGGTCTT TGACTCAGAA GGAGGTCACA 
GGAGTGTGTC CTGGAGAGGC CCTCCAGACC AAACCGGCTG GCTGTGGTCG GAGAATCTCT 
GAGACCTCGG TGTCTACTCC GGGCCCCTCC CTCCCTGAAG ATGACGTGTT CCTGAGAGAA 
GCCAAAACAC CGTCACCTCA AGATTCCCAA GGGCTCCCCA CCAGCACCTC CTACCGGCAG 
TATGAAAACG ACTTAAGCAA AAAAGCTGGT CAGATTGCAG TTTCAGCAGA GAGACCCCTC 
CATGAGACTC CAGGGATCAC GGGTACAGAG GACTGTGGGC AAGCGGTGAA CGGCTCTGTG 
GATCTCTCCA GACCCACAAG CATTCCAGAG ACTACAAATG ACGACATCCC AACCTTTGAC 
ACTAATGGGA CCACTGACCC CTCTGCAGCT ACAGAGAAAA AACCCCTCAA GCCTCCCCCA 
GTCGATGTCT TGAGACCTTC AGACTCTGAG ACTCCAGGGT CCCCTCACCA CACTTCCCTG 
ACTTGGGGCC AGTTTGATTC CAAGACTACT TGGCCTAGTC GGCATTTTGA GGCGCTGGTT 
CAAGAGCTGG CCAGACTGGA TCCCTCTTTG AGCCGTACTC TTGCCGCCCA GCCCGGTCCA 
GAGCCACCGC AGGGCCTGCT GGATGGGCTT TTTCCTGTCG AAGAGATCCG AAGTGCGATG 
AGGCCAGCCC TTGAGGAGAT GGGAGAGAAG GCTGCTGGCG CATCGGAGGA AGGGTCTTGT 
GGACACCACC TCACGCAGGA CCTGCAGACT TCCCAAGAGG CATCAAGGTC TGAAAACTCT 
ACCCCCGACC CTGACCAATC AAGTGGCCAG GAATTCCCAG AAGGAAACAG CACCCAGGCC 
AAGAAAGTGG AACTAGCCCG CCTCCTCCAA AAGATGCTGC AGGATCTTCA CGCTGAGCAG 
GAGCGACTGC GGGGGACCGC CGCAGACTGG ACCCAACGCA ACGGAGCTCT AGAGGCTGCT 
GTGAGCCAGG CCTGTACACC CCGGGAGCTG GAACGGTTCC GCCGGTTCAT GACTGATCTA 
GAGCGTGTGC TTGGTCTGCT GCTGCTGCTA GGCAGTCGCC TGGTCCGTGT AAACCTCGCC 
TTGGCTAGGG CGGGCTCAAA CAGCGACCCT GATGAGAGGG CCTCTCTGCT GCAGCGACTC 
CAACTTCTGC AGCGACAGCA AGAAGAAGCT AAGGAGCTGA AGGAGCACGT GGCTCGGCGG 
GAGCAAACCC TGCGTCAGGT GTTGGAGCGG GAGCTGCCCG CAGAGCATCT GCGCTCCTAT 
TGTGTGCTGC TGGCCTCCAA GGCCAGGATC CTGTCACAGC AGCGCAGCCT GGACGATCGA 
ATCCGGTTCC TTAAGGACCA ACTGGACACT ATCTGGAGCG ACCTCAGCCA TCATCCCCTT 
TCTCCCAGAC TGACCTGGGC CCCAGCAATC CGTCCTCTAA ACAAACAACC TTTTCTTGCT 
ACCCTTATCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_082193.2
CDS	170..2641
Translation	MEALGTGRDRTSQASATESLDLRRLSTRADSAYSSFSTASGDPETRTPSPGTERLPYLDWDYVRVVWGSQSPTSKDAVLSTTQRPVQAVAGHSDPRSPEVQGSPGPLNRQDTPLLYALAAEAEATAHTAEPPSPPASRDAYRQRLQGAQRRVLRETSFQRKEFRMSLPGRLRPAVPTRLPTAHVRSASSSQELGEEEPARTAVPALAAAGRGRLSSQQRQCCFSEPGKLHRVGWSGGPTGEDLRKDYSTQELQRGMHAKSKGLLETQSLSSTELNSGPADLGNAHRPAGRSQSVSGEVMGPCKGSERTVATVQAVPQRADIRRPLLHTKLSRSLTQKEVTGVCPGEALQTKPAGCGRRISETSVSTPGPSLPEDDVFLREAKTPSPQDSQGLPTSTSYRQYENDLSKKAGQIAVSAERPLHETPGITGTEDCGQAVNGSVDLSRPTSIPETTNDDIPTFDTNGTTDPSAATEKKPLKPPPVDVLRPSDSETPGSPHHTSLTWGQFDSKTTWPSRHFEALVQELARLDPSLSRTLAAQPGPEPPQGLLDGLFPVEEIRSAMRPALEEMGEKAAGASEEGSCGHHLTQDLQTSQEASRSENSTPDPDQSSGQEFPEGNSTQAKKVELARLLQKMLQDLHAEQERLRGTAADWTQRNGALEAAVSQACTPRELERFRRFMTDLERVLGLLLLLGSRLVRVNLALARAGSNSDPDERASLLQRLQLLQRQQEEAKELKEHVARREQTLRQVLERELPAEHLRSYCVLLASKARILSQQRSLDDRIRFLKDQLDTIWSDLSHHPLSPRLTWAPAIRPLNKQPFLATLI

Target ORF information:

RefSeq Version	NM_027917.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus shroom family member 1 (Shroom1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_027917.3

ORF Insert Sequence:

ATGGAGGCCC TGGGTACTGG GAGAGACCGC ACCTCCCAGG CCTCAGCCAC TGAAAGCCTG 
GACTTGCGAC GGTTGTCCAC GCGCGCCGAC TCTGCCTACA GCTCTTTCTC CACGGCGTCT 
GGTGATCCTG AGACGCGCAC TCCGTCCCCT GGCACCGAGC GCCTCCCTTA CCTAGACTGG 
GACTACGTGC GAGTGGTTTG GGGCAGCCAA TCTCCTACCT CGAAGGACGC TGTCCTTTCA 
ACGACTCAGC GACCTGTGCA GGCAGTCGCT GGGCACAGTG ACCCACGGTC CCCAGAGGTC 
CAGGGAAGCC CGGGACCACT AAACAGACAA GACACCCCAC TGCTGTACGC GCTGGCCGCG 
GAGGCTGAAG CTACAGCTCA CACCGCAGAG CCGCCCAGCC CGCCAGCCTC GCGGGACGCC 
TACCGCCAGC GGTTGCAGGG TGCACAGCGA CGAGTGCTGC GGGAGACGTC CTTCCAGCGA 
AAGGAGTTTC GCATGAGCCT GCCGGGCCGC CTGCGTCCCG CAGTCCCCAC GCGACTTCCC 
ACGGCGCACG TGCGCTCCGC CTCCAGCAGC CAGGAGCTGG GAGAAGAGGA GCCGGCGCGC 
ACCGCTGTCC CAGCGCTAGC TGCTGCTGGC CGGGGGCGTC TCTCCAGCCA GCAGCGGCAG 
TGCTGCTTCT CTGAGCCAGG CAAATTACAT CGCGTAGGTT GGAGCGGTGG GCCCACTGGT 
GAGGACTTGA GAAAAGATTA TTCCACGCAG GAGTTGCAGC GTGGGATGCA CGCAAAGTCC 
AAAGGGCTGC TGGAGACTCA GTCCCTAAGC TCAACGGAGC TGAATTCCGG GCCAGCGGAT 
CTTGGCAATG CCCATAGACC TGCGGGTCGG AGTCAGAGTG TTTCAGGCGA GGTCATGGGT 
CCCTGCAAGG GTTCAGAAAG GACTGTGGCC ACTGTCCAGG CTGTTCCTCA AAGAGCAGAC 
ATCCGCAGAC CACTGCTTCA CACCAAGCTT TCCAGGTCTT TGACTCAGAA GGAGGTCACA 
GGAGTGTGTC CTGGAGAGGC CCTCCAGACC AAACCGGCTG GCTGTGGTCG GAGAATCTCT 
GAGACCTCGG TGTCTACTCC GGGCCCCTCC CTCCCTGAAG ATGACGTGTT CCTGAGAGAA 
GCCAAAACAC CGTCACCTCA AGATTCCCAA GGGCTCCCCA CCAGCACCTC CTACCGGCAG 
TATGAAAACG ACTTAAGCAA AAAAGCTGGT CAGATTGCAG TTTCAGCAGA GAGACCCCTC 
CATGAGACTC CAGGGATCAC GGGTACAGAG GACTGTGGGC AAGCGGTGAA CGGCTCTGTG 
GATCTCTCCA GACCCACAAG CATTCCAGAG ACTACAAATG ACGACATCCC AACCTTTGAC 
ACTAATGGGA CCACTGACCC CTCTGCAGCT ACAGAGAAAA AACCCCTCAA GCCTCCCCCA 
GTCGATGTCT TGAGACCTTC AGACTCTGAG ACTCCAGGGT CCCCTCACCA CACTTCCCTG 
ACTTGGGGCC AGTTTGATTC CAAGACTACT TGGCCTAGTC GGCATTTTGA GGCGCTGGTT 
CAAGAGCTGG CCAGACTGGA TCCCTCTTTG AGCCGTACTC TTGCCGCCCA GCCCGGTCCA 
GAGCCACCGC AGGGCCTGCT GGATGGGCTT TTTCCTGTCG AAGAGATCCG AAGTGCGATG 
AGGCCAGCCC TTGAGGAGAT GGGAGAGAAG GCTGCTGGCG CATCGGAGGA AGGGTCTTGT 
GGACACCACC TCACGCAGGA CCTGCAGACT TCCCAAGAGG CATCAAGGTC TGAAAACTCT 
ACCCCCGACC CTGACCAATC AAGTGGCCAG GAATTCCCAG AAGGAAACAG CACCCAGGCC 
AAGAAAGTGG AACTAGCCCG CCTCCTCCAA AAGATGCTGC AGGATCTTCA CGCTGAGCAG 
GAGCGACTGC GGGGGACCGC CGCAGACTGG ACCCAACGCA ACGGAGCTCT AGAGGCTGCT 
GTGAGCCAGG CCTGTACACC CCGGGAGCTG GAACGGTTCC GCCGGTTCAT GACTGATCTA 
GAGCGTGTGC TTGGTCTGCT GCTGCTGCTA GGCAGTCGCC TGGTCCGTGT AAACCTCGCC 
TTGGCTAGGG CGGGCTCAAA CAGCGACCCT GATGAGAGGG CCTCTCTGCT GCAGCGACTC 
CAACTTCTGC AGCGACAGCA AGAAGAAGCT AAGGAGCTGA AGGAGCACGT GGCTCGGCGG 
GAGCAAACCC TGCGTCAGGT GTTGGAGCGG GAGCTGCCCG CAGAGCATCT GCGCTCCTAT 
TGTGTGCTGC TGGCCTCCAA GGCCAGGATC CTGTCACAGC AGCGCAGCCT GGACGATCGA 
ATCCGGTTCC TTAAGGACCA ACTGGACACT ATCTGGAGCG ACCTCAGCCA TCATCCCCTT 
TCTCCCAGAC TGACCTGGGC CCCAGCAATC CGTCCTCTAA ACAAACAACC TTTTCTTGCT 
ACCCTTATCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Shroom1 ( XM_006534259.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006534322.1 Mus musculus shroom family member 1 (Shroom1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu46955
Clone ID Related Accession (Same CDS sequence)	XM_006534259.3 , XM_006534259.4
Accession Version	XM_006534259.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2415bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein Shroom1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006534259.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCCC TGGGTACTGG GAGAGACCGC ACCTCCCAGG CCTCAGCCAC TGAAAGCCTG 
GACTTGCGAC GGTTGTCCAC GCGCGCCGAC TCTGCCTACA GCTCTTTCTC CACGGCGTCT 
GGTGATCCTG AGACGCGCAC TCCGTCCCCT GGCACCGAGC GCCTCCCTTA CCTAGACTGG 
GACTACGTGC GAGTGGTTTG GGGCAGCCAA TCTCCTACCT CGAAGGACGC TGTCCTTTCA 
ACGACTCAGC GACCTGTGCA GGCAGTCGCT GGGCACAGTG ACCCACGGTC CCCAGAGGTC 
CAGGGAAGCC CGGGACCACT AAACAGACAA GACACCCCAC TGCTGTACGC GCTGGCCGCG 
GAGGCTGAAG CTACAGCTCA CACCGCAGAG CCGCCCAGCC CGCCAGCCTC GCGGGACGCC 
TACCGCCAGC GGTTGCAGGG TGCACAGCGA CGAGTGCTGC GGGAGACGTC CTTCCAGCGA 
AAGGAGTTTC GCATGAGCCT GCCGGGCCGC CTGCGTCCCG CAGTCCCCAC GCGACTTCCC 
ACGGCGCACG TGCGCTCCGC CTCCAGCAGC CAGGAGCTGG GAGAAGAGGA GCCGGCGCGC 
ACCGCTGTCC CAGCGCTAGC TGCTGCTGGC CGGGGGCGTC TCTCCAGCCA GCAGCGGCAG 
TGCTGCTTCT CTGAGCCAGG CAAATTACAT CGCGTAGGTT GGAGCGGTGG GCCCACTGGT 
GAGGACTTGA GAAAAGATTA TTCCACGCAG GAGTTGCAGC GTGGGATGCA CGCAAAGTCC 
AAAGGGCTGC TGGAGACTCA GTCCCTAAGC TCAACGGAGC TGAATTCCGG GCCAGCGGAT 
CTTGGCAATG CCCATAGACC TGCGGGTCGG AGTCAGAGTG TTTCAGGCGA GGTCATGGGT 
CCCTGCAAGG GTTCAGAAAG GACTGTGGCC ACTGTCCAGG CTGTTCCTCA AAGAGCAGAC 
ATCCGCAGAC CACTGCTTCA CACCAAGCTT TCCAGGTCTT TGACTCAGAA GGAGGTCACA 
GGAGTGTGTC CTGGAGAGGC CCTCCAGACC AAACCGGCTG GCTGTGGTCG GAGAATCTCT 
GAGACCTCGG TGTCTACTCC GGGCCCCTCC CTCCCTGAAG ATGACGTGTT CCTGAGAGAA 
GCCAAAACAC CGTCACCTCA AGATTCCCAA GGGCTCCCCA CCAGCACCTC CTACCGGCAG 
TATGAAAACG ACTTAAGCAA AAAAGCTGGT CAGATTGCAG TTTCAGCAGA GAGACCCCTC 
CATGAGACTC CAGGGATCAC GGGTACAGAG GACTGTGGGC AAGCGGTGAA CGGCTCTGTG 
GATCTCTCCA GACCCACAAG CATTCCAGAG ACTACAAATG ACGACATCCC AACCTTTGAC 
ACTAATGGGA CCACTGACCC CTCTGCAGCT ACAGAGAAAA AACCCCTCAA GCCTCCCCCA 
GTCGATGTCT TGAGACCTTC AGACTCTGAG ACTCCAGGGT CCCCTCACCA CACTTCCCTG 
ACTTGGGGCC AGTTTGATTC CAAGACTACT TGGCCTAGTC GGCATTTTGA GGCGCTGGTT 
CAAGAGCTGG CCAGACTGGA TCCCTCTTTG AGCCGTACTC TTGCCGCCCA GCCCGGTCCA 
GAGCCACCGC AGGGCCTGCT GGATGGGCTT TTTCCTGTCG AAGAGATCCG AAGTGCGATG 
AGGCCAGCCC TTGAGGAGAT GGGAGAGAAG GCTGCTGGCG CATCGGAGGA AGGGTCTTGT 
GGACACCACC TCACGCAGGA CCTGCAGACT TCCCAAGAGG CATCAAGGTC TGAAAACTCT 
ACCCCCGACC CTGACCAATC AAGTGGCCAG GAATTCCCAG AAGGAAACAG CACCCAGGCC 
AAGAAAGTGG AACTAGCCCG CCTCCTCCAA AAGATGCTGC AGGATCTTCA CGCTGAGCAG 
GAGCGACTGC GGGGGACCGC CGCAGACTGG ACCCAACGCA ACGGAGCTCT AGAGGCTGCT 
GTGAGCCAGG CCTGTACACC CCGGGAGCTG GAACGGTTCC GCCGGTTCAT GACTGATCTA 
GAGCGTGTGC TTGGTCTGCT GCTGCTGCTA GGCAGTCGCC TGGCCTCTCT GCTGCAGCGA 
CTCCAACTTC TGCAGCGACA GCAAGAAGAA GCTAAGGAGC TGAAGGAGCA CGTGGCTCGG 
CGGGAGCAAA CCCTGCGTCA GGTGTTGGAG CGGGAGCTGC CCGCAGAGCA TCTGCGCTCC 
TATTGTGTGC TGCTGGCCTC CAAGGCCAGG ATCCTGTCAC AGCAGCGCAG CCTGGACGAT 
CGAATCCGGT TCCTTAAGGA CCAACTGGAC ACTATCTGGA GCGACCTCAG CCATCATCCC 
CTTTCTCCCA GACTGACCTG GGCCCCAGCA ATCCGTCCTC TAAACAAACA ACCTTTTCTT 
GCTACCCTTA TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006534322.1
CDS	668..3082
Translation	MEALGTGRDRTSQASATESLDLRRLSTRADSAYSSFSTASGDPETRTPSPGTERLPYLDWDYVRVVWGSQSPTSKDAVLSTTQRPVQAVAGHSDPRSPEVQGSPGPLNRQDTPLLYALAAEAEATAHTAEPPSPPASRDAYRQRLQGAQRRVLRETSFQRKEFRMSLPGRLRPAVPTRLPTAHVRSASSSQELGEEEPARTAVPALAAAGRGRLSSQQRQCCFSEPGKLHRVGWSGGPTGEDLRKDYSTQELQRGMHAKSKGLLETQSLSSTELNSGPADLGNAHRPAGRSQSVSGEVMGPCKGSERTVATVQAVPQRADIRRPLLHTKLSRSLTQKEVTGVCPGEALQTKPAGCGRRISETSVSTPGPSLPEDDVFLREAKTPSPQDSQGLPTSTSYRQYENDLSKKAGQIAVSAERPLHETPGITGTEDCGQAVNGSVDLSRPTSIPETTNDDIPTFDTNGTTDPSAATEKKPLKPPPVDVLRPSDSETPGSPHHTSLTWGQFDSKTTWPSRHFEALVQELARLDPSLSRTLAAQPGPEPPQGLLDGLFPVEEIRSAMRPALEEMGEKAAGASEEGSCGHHLTQDLQTSQEASRSENSTPDPDQSSGQEFPEGNSTQAKKVELARLLQKMLQDLHAEQERLRGTAADWTQRNGALEAAVSQACTPRELERFRRFMTDLERVLGLLLLLGSRLASLLQRLQLLQRQQEEAKELKEHVARREQTLRQVLERELPAEHLRSYCVLLASKARILSQQRSLDDRIRFLKDQLDTIWSDLSHHPLSPRLTWAPAIRPLNKQPFLATLI

Target ORF information:

RefSeq Version	XM_006534259.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus shroom family member 1 (Shroom1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006534259.3

ORF Insert Sequence:

ATGGAGGCCC TGGGTACTGG GAGAGACCGC ACCTCCCAGG CCTCAGCCAC TGAAAGCCTG 
GACTTGCGAC GGTTGTCCAC GCGCGCCGAC TCTGCCTACA GCTCTTTCTC CACGGCGTCT 
GGTGATCCTG AGACGCGCAC TCCGTCCCCT GGCACCGAGC GCCTCCCTTA CCTAGACTGG 
GACTACGTGC GAGTGGTTTG GGGCAGCCAA TCTCCTACCT CGAAGGACGC TGTCCTTTCA 
ACGACTCAGC GACCTGTGCA GGCAGTCGCT GGGCACAGTG ACCCACGGTC CCCAGAGGTC 
CAGGGAAGCC CGGGACCACT AAACAGACAA GACACCCCAC TGCTGTACGC GCTGGCCGCG 
GAGGCTGAAG CTACAGCTCA CACCGCAGAG CCGCCCAGCC CGCCAGCCTC GCGGGACGCC 
TACCGCCAGC GGTTGCAGGG TGCACAGCGA CGAGTGCTGC GGGAGACGTC CTTCCAGCGA 
AAGGAGTTTC GCATGAGCCT GCCGGGCCGC CTGCGTCCCG CAGTCCCCAC GCGACTTCCC 
ACGGCGCACG TGCGCTCCGC CTCCAGCAGC CAGGAGCTGG GAGAAGAGGA GCCGGCGCGC 
ACCGCTGTCC CAGCGCTAGC TGCTGCTGGC CGGGGGCGTC TCTCCAGCCA GCAGCGGCAG 
TGCTGCTTCT CTGAGCCAGG CAAATTACAT CGCGTAGGTT GGAGCGGTGG GCCCACTGGT 
GAGGACTTGA GAAAAGATTA TTCCACGCAG GAGTTGCAGC GTGGGATGCA CGCAAAGTCC 
AAAGGGCTGC TGGAGACTCA GTCCCTAAGC TCAACGGAGC TGAATTCCGG GCCAGCGGAT 
CTTGGCAATG CCCATAGACC TGCGGGTCGG AGTCAGAGTG TTTCAGGCGA GGTCATGGGT 
CCCTGCAAGG GTTCAGAAAG GACTGTGGCC ACTGTCCAGG CTGTTCCTCA AAGAGCAGAC 
ATCCGCAGAC CACTGCTTCA CACCAAGCTT TCCAGGTCTT TGACTCAGAA GGAGGTCACA 
GGAGTGTGTC CTGGAGAGGC CCTCCAGACC AAACCGGCTG GCTGTGGTCG GAGAATCTCT 
GAGACCTCGG TGTCTACTCC GGGCCCCTCC CTCCCTGAAG ATGACGTGTT CCTGAGAGAA 
GCCAAAACAC CGTCACCTCA AGATTCCCAA GGGCTCCCCA CCAGCACCTC CTACCGGCAG 
TATGAAAACG ACTTAAGCAA AAAAGCTGGT CAGATTGCAG TTTCAGCAGA GAGACCCCTC 
CATGAGACTC CAGGGATCAC GGGTACAGAG GACTGTGGGC AAGCGGTGAA CGGCTCTGTG 
GATCTCTCCA GACCCACAAG CATTCCAGAG ACTACAAATG ACGACATCCC AACCTTTGAC 
ACTAATGGGA CCACTGACCC CTCTGCAGCT ACAGAGAAAA AACCCCTCAA GCCTCCCCCA 
GTCGATGTCT TGAGACCTTC AGACTCTGAG ACTCCAGGGT CCCCTCACCA CACTTCCCTG 
ACTTGGGGCC AGTTTGATTC CAAGACTACT TGGCCTAGTC GGCATTTTGA GGCGCTGGTT 
CAAGAGCTGG CCAGACTGGA TCCCTCTTTG AGCCGTACTC TTGCCGCCCA GCCCGGTCCA 
GAGCCACCGC AGGGCCTGCT GGATGGGCTT TTTCCTGTCG AAGAGATCCG AAGTGCGATG 
AGGCCAGCCC TTGAGGAGAT GGGAGAGAAG GCTGCTGGCG CATCGGAGGA AGGGTCTTGT 
GGACACCACC TCACGCAGGA CCTGCAGACT TCCCAAGAGG CATCAAGGTC TGAAAACTCT 
ACCCCCGACC CTGACCAATC AAGTGGCCAG GAATTCCCAG AAGGAAACAG CACCCAGGCC 
AAGAAAGTGG AACTAGCCCG CCTCCTCCAA AAGATGCTGC AGGATCTTCA CGCTGAGCAG 
GAGCGACTGC GGGGGACCGC CGCAGACTGG ACCCAACGCA ACGGAGCTCT AGAGGCTGCT 
GTGAGCCAGG CCTGTACACC CCGGGAGCTG GAACGGTTCC GCCGGTTCAT GACTGATCTA 
GAGCGTGTGC TTGGTCTGCT GCTGCTGCTA GGCAGTCGCC TGGCCTCTCT GCTGCAGCGA 
CTCCAACTTC TGCAGCGACA GCAAGAAGAA GCTAAGGAGC TGAAGGAGCA CGTGGCTCGG 
CGGGAGCAAA CCCTGCGTCA GGTGTTGGAG CGGGAGCTGC CCGCAGAGCA TCTGCGCTCC 
TATTGTGTGC TGCTGGCCTC CAAGGCCAGG ATCCTGTCAC AGCAGCGCAG CCTGGACGAT 
CGAATCCGGT TCCTTAAGGA CCAACTGGAC ACTATCTGGA GCGACCTCAG CCATCATCCC 
CTTTCTCCCA GACTGACCTG GGCCCCAGCA ATCCGTCCTC TAAACAAACA ACCTTTTCTT 
GCTACCCTTA TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Shroom1 ( NM_001290789.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001277718.1 Mus musculus shroom family member 1 (Shroom1), transcript variant 2, mRNA.

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CloneID	OMu17427
Clone ID Related Accession (Same CDS sequence)	NM_001290789.1
Accession Version	NM_001290789.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2283bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Mus musculus(house mouse)
Product	protein Shroom1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL596095.18, AK134332.1 and AK035462.1. On Mar 20, 2014 this sequence version replaced XM_006534260.1. Transcript Variant: This variant (2) lacks an exon in the central coding region but maintains reading frame, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC005761.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGAGGCCC TGGGTACTGG GAGAGACCGC ACCTCCCAGG CCTCAGCCAC TGAAAGCCTG 
GACTTGCGAC GGTTGTCCAC GCGCGCCGAC TCTGCCTACA GCTCTTTCTC CACGGCGTCT 
GGTGATCCTG AGACGCGCAC TCCGTCCCCT GGCACCGAGC GCCTCCCTTA CCTAGACTGG 
GACTACGTGC GAGTGGTTTG GGGCAGCCAA TCTCCTACCT CGAAGGACGC TGTCCTTTCA 
ACGACTCAGC GACCTGTGCA GGCAGTCGCT GGGCACAGTG ACCCACGGTC CCCAGAGGTC 
CAGGGAAGCC CGGGACCACT AAACAGACAA GACACCCCAC TGCTGTACGC GCTGGCCGCG 
GAGGCTGAAG CTACAGCTCA CACCGCAGAG CCGCCCAGCC CGCCAGCCTC GCGGGACGCC 
TACCGCCAGC GGTTGCAGGG TGCACAGCGA CGAGTGCTGC GGGAGACGTC CTTCCAGCGA 
AAGGAGTTTC GCATGAGCCT GCCGGGCCGC CTGCGTCCCG CAGTCCCCAC GCGACTTCCC 
ACGGCGCACG TGCGCTCCGC CTCCAGCAGC CAGGAGCTGG GAGAAGAGGA GCCGGCGCGC 
ACCGCTGTCC CAGCGCTAGC TGCTGCTGGC CGGGGGCGTC TCTCCAGCCA GCAGCGGCAG 
TGCTGCTTCT CTGAGCCAGG CAAATTACAT CGCGTAGGTT GGAGCGGTGG GCCCACTGGT 
GAGGACTTGA GAAAAGATTA TTCCACGCAG GAGTTGCAGC GTGGGATGCA CGCAAAGTCC 
AAAGGGCTGC TGGAGACTCA GTCCCTAAGC TCAACGGAGC TGAATTCCGG GCCAGCGGAT 
CTTGGCAATG CCCATAGACC TGCGGGTCGG AGTCAGAGTG TTTCAGGCGA GGTCATGGGT 
CCCTGCAAGG GTTCAGAAAG GACTGTGGCC ACTGTCCAGG CTGTTCCTCA AAGAGCAGAC 
ATCCGCAGAC CACTGCTTCA CACCAAGCTT TCCAGCACCT CCTACCGGCA GTATGAAAAC 
GACTTAAGCA AAAAAGCTGG TCAGATTGCA GTTTCAGCAG AGAGACCCCT CCATGAGACT 
CCAGGGATCA CGGGTACAGA GGACTGTGGG CAAGCGGTGA ACGGCTCTGT GGATCTCTCC 
AGACCCACAA GCATTCCAGA GACTACAAAT GACGACATCC CAACCTTTGA CACTAATGGG 
ACCACTGACC CCTCTGCAGC TACAGAGAAA AAACCCCTCA AGCCTCCCCC AGTCGATGTC 
TTGAGACCTT CAGACTCTGA GACTCCAGGG TCCCCTCACC ACACTTCCCT GACTTGGGGC 
CAGTTTGATT CCAAGACTAC TTGGCCTAGT CGGCATTTTG AGGCGCTGGT TCAAGAGCTG 
GCCAGACTGG ATCCCTCTTT GAGCCGTACT CTTGCCGCCC AGCCCGGTCC AGAGCCACCG 
CAGGGCCTGC TGGATGGGCT TTTTCCTGTC GAAGAGATCC GAAGTGCGAT GAGGCCAGCC 
CTTGAGGAGA TGGGAGAGAA GGCTGCTGGC GCATCGGAGG AAGGGTCTTG TGGACACCAC 
CTCACGCAGG ACCTGCAGAC TTCCCAAGAG GCATCAAGGT CTGAAAACTC TACCCCCGAC 
CCTGACCAAT CAAGTGGCCA GGAATTCCCA GAAGGAAACA GCACCCAGGC CAAGAAAGTG 
GAACTAGCCC GCCTCCTCCA AAAGATGCTG CAGGATCTTC ACGCTGAGCA GGAGCGACTG 
CGGGGGACCG CCGCAGACTG GACCCAACGC AACGGAGCTC TAGAGGCTGC TGTGAGCCAG 
GCCTGTACAC CCCGGGAGCT GGAACGGTTC CGCCGGTTCA TGACTGATCT AGAGCGTGTG 
CTTGGTCTGC TGCTGCTGCT AGGCAGTCGC CTGGTCCGTG TAAACCTCGC CTTGGCTAGG 
GCGGGCTCAA ACAGCGACCC TGATGAGAGG GCCTCTCTGC TGCAGCGACT CCAACTTCTG 
CAGCGACAGC AAGAAGAAGC TAAGGAGCTG AAGGAGCACG TGGCTCGGCG GGAGCAAACC 
CTGCGTCAGG TGTTGGAGCG GGAGCTGCCC GCAGAGCATC TGCGCTCCTA TTGTGTGCTG 
CTGGCCTCCA AGGCCAGGAT CCTGTCACAG CAGCGCAGCC TGGACGATCG AATCCGGTTC 
CTTAAGGACC AACTGGACAC TATCTGGAGC GACCTCAGCC ATCATCCCCT TTCTCCCAGA 
CTGACCTGGG CCCCAGCAAT CCGTCCTCTA AACAAACAAC CTTTTCTTGC TACCCTTATC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001277718.1
CDS	170..2452
Translation	MEALGTGRDRTSQASATESLDLRRLSTRADSAYSSFSTASGDPETRTPSPGTERLPYLDWDYVRVVWGSQSPTSKDAVLSTTQRPVQAVAGHSDPRSPEVQGSPGPLNRQDTPLLYALAAEAEATAHTAEPPSPPASRDAYRQRLQGAQRRVLRETSFQRKEFRMSLPGRLRPAVPTRLPTAHVRSASSSQELGEEEPARTAVPALAAAGRGRLSSQQRQCCFSEPGKLHRVGWSGGPTGEDLRKDYSTQELQRGMHAKSKGLLETQSLSSTELNSGPADLGNAHRPAGRSQSVSGEVMGPCKGSERTVATVQAVPQRADIRRPLLHTKLSSTSYRQYENDLSKKAGQIAVSAERPLHETPGITGTEDCGQAVNGSVDLSRPTSIPETTNDDIPTFDTNGTTDPSAATEKKPLKPPPVDVLRPSDSETPGSPHHTSLTWGQFDSKTTWPSRHFEALVQELARLDPSLSRTLAAQPGPEPPQGLLDGLFPVEEIRSAMRPALEEMGEKAAGASEEGSCGHHLTQDLQTSQEASRSENSTPDPDQSSGQEFPEGNSTQAKKVELARLLQKMLQDLHAEQERLRGTAADWTQRNGALEAAVSQACTPRELERFRRFMTDLERVLGLLLLLGSRLVRVNLALARAGSNSDPDERASLLQRLQLLQRQQEEAKELKEHVARREQTLRQVLERELPAEHLRSYCVLLASKARILSQQRSLDDRIRFLKDQLDTIWSDLSHHPLSPRLTWAPAIRPLNKQPFLATLI

Target ORF information:

RefSeq Version	NM_001290789.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus shroom family member 1 (Shroom1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001290789.1

ORF Insert Sequence:

ATGGAGGCCC TGGGTACTGG GAGAGACCGC ACCTCCCAGG CCTCAGCCAC TGAAAGCCTG 
GACTTGCGAC GGTTGTCCAC GCGCGCCGAC TCTGCCTACA GCTCTTTCTC CACGGCGTCT 
GGTGATCCTG AGACGCGCAC TCCGTCCCCT GGCACCGAGC GCCTCCCTTA CCTAGACTGG 
GACTACGTGC GAGTGGTTTG GGGCAGCCAA TCTCCTACCT CGAAGGACGC TGTCCTTTCA 
ACGACTCAGC GACCTGTGCA GGCAGTCGCT GGGCACAGTG ACCCACGGTC CCCAGAGGTC 
CAGGGAAGCC CGGGACCACT AAACAGACAA GACACCCCAC TGCTGTACGC GCTGGCCGCG 
GAGGCTGAAG CTACAGCTCA CACCGCAGAG CCGCCCAGCC CGCCAGCCTC GCGGGACGCC 
TACCGCCAGC GGTTGCAGGG TGCACAGCGA CGAGTGCTGC GGGAGACGTC CTTCCAGCGA 
AAGGAGTTTC GCATGAGCCT GCCGGGCCGC CTGCGTCCCG CAGTCCCCAC GCGACTTCCC 
ACGGCGCACG TGCGCTCCGC CTCCAGCAGC CAGGAGCTGG GAGAAGAGGA GCCGGCGCGC 
ACCGCTGTCC CAGCGCTAGC TGCTGCTGGC CGGGGGCGTC TCTCCAGCCA GCAGCGGCAG 
TGCTGCTTCT CTGAGCCAGG CAAATTACAT CGCGTAGGTT GGAGCGGTGG GCCCACTGGT 
GAGGACTTGA GAAAAGATTA TTCCACGCAG GAGTTGCAGC GTGGGATGCA CGCAAAGTCC 
AAAGGGCTGC TGGAGACTCA GTCCCTAAGC TCAACGGAGC TGAATTCCGG GCCAGCGGAT 
CTTGGCAATG CCCATAGACC TGCGGGTCGG AGTCAGAGTG TTTCAGGCGA GGTCATGGGT 
CCCTGCAAGG GTTCAGAAAG GACTGTGGCC ACTGTCCAGG CTGTTCCTCA AAGAGCAGAC 
ATCCGCAGAC CACTGCTTCA CACCAAGCTT TCCAGCACCT CCTACCGGCA GTATGAAAAC 
GACTTAAGCA AAAAAGCTGG TCAGATTGCA GTTTCAGCAG AGAGACCCCT CCATGAGACT 
CCAGGGATCA CGGGTACAGA GGACTGTGGG CAAGCGGTGA ACGGCTCTGT GGATCTCTCC 
AGACCCACAA GCATTCCAGA GACTACAAAT GACGACATCC CAACCTTTGA CACTAATGGG 
ACCACTGACC CCTCTGCAGC TACAGAGAAA AAACCCCTCA AGCCTCCCCC AGTCGATGTC 
TTGAGACCTT CAGACTCTGA GACTCCAGGG TCCCCTCACC ACACTTCCCT GACTTGGGGC 
CAGTTTGATT CCAAGACTAC TTGGCCTAGT CGGCATTTTG AGGCGCTGGT TCAAGAGCTG 
GCCAGACTGG ATCCCTCTTT GAGCCGTACT CTTGCCGCCC AGCCCGGTCC AGAGCCACCG 
CAGGGCCTGC TGGATGGGCT TTTTCCTGTC GAAGAGATCC GAAGTGCGAT GAGGCCAGCC 
CTTGAGGAGA TGGGAGAGAA GGCTGCTGGC GCATCGGAGG AAGGGTCTTG TGGACACCAC 
CTCACGCAGG ACCTGCAGAC TTCCCAAGAG GCATCAAGGT CTGAAAACTC TACCCCCGAC 
CCTGACCAAT CAAGTGGCCA GGAATTCCCA GAAGGAAACA GCACCCAGGC CAAGAAAGTG 
GAACTAGCCC GCCTCCTCCA AAAGATGCTG CAGGATCTTC ACGCTGAGCA GGAGCGACTG 
CGGGGGACCG CCGCAGACTG GACCCAACGC AACGGAGCTC TAGAGGCTGC TGTGAGCCAG 
GCCTGTACAC CCCGGGAGCT GGAACGGTTC CGCCGGTTCA TGACTGATCT AGAGCGTGTG 
CTTGGTCTGC TGCTGCTGCT AGGCAGTCGC CTGGTCCGTG TAAACCTCGC CTTGGCTAGG 
GCGGGCTCAA ACAGCGACCC TGATGAGAGG GCCTCTCTGC TGCAGCGACT CCAACTTCTG 
CAGCGACAGC AAGAAGAAGC TAAGGAGCTG AAGGAGCACG TGGCTCGGCG GGAGCAAACC 
CTGCGTCAGG TGTTGGAGCG GGAGCTGCCC GCAGAGCATC TGCGCTCCTA TTGTGTGCTG 
CTGGCCTCCA AGGCCAGGAT CCTGTCACAG CAGCGCAGCC TGGACGATCG AATCCGGTTC 
CTTAAGGACC AACTGGACAC TATCTGGAGC GACCTCAGCC ATCATCCCCT TTCTCCCAGA 
CTGACCTGGG CCCCAGCAAT CCGTCCTCTA AACAAACAAC CTTTTCTTGC TACCCTTATC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Shroom1 ( XM_006534259.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006534322.1 Mus musculus shroom family member 1 (Shroom1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu46955
Clone ID Related Accession (Same CDS sequence)	XM_006534259.3 , XM_006534259.4
Accession Version	XM_006534259.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2415bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein Shroom1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006534259.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCCC TGGGTACTGG GAGAGACCGC ACCTCCCAGG CCTCAGCCAC TGAAAGCCTG 
GACTTGCGAC GGTTGTCCAC GCGCGCCGAC TCTGCCTACA GCTCTTTCTC CACGGCGTCT 
GGTGATCCTG AGACGCGCAC TCCGTCCCCT GGCACCGAGC GCCTCCCTTA CCTAGACTGG 
GACTACGTGC GAGTGGTTTG GGGCAGCCAA TCTCCTACCT CGAAGGACGC TGTCCTTTCA 
ACGACTCAGC GACCTGTGCA GGCAGTCGCT GGGCACAGTG ACCCACGGTC CCCAGAGGTC 
CAGGGAAGCC CGGGACCACT AAACAGACAA GACACCCCAC TGCTGTACGC GCTGGCCGCG 
GAGGCTGAAG CTACAGCTCA CACCGCAGAG CCGCCCAGCC CGCCAGCCTC GCGGGACGCC 
TACCGCCAGC GGTTGCAGGG TGCACAGCGA CGAGTGCTGC GGGAGACGTC CTTCCAGCGA 
AAGGAGTTTC GCATGAGCCT GCCGGGCCGC CTGCGTCCCG CAGTCCCCAC GCGACTTCCC 
ACGGCGCACG TGCGCTCCGC CTCCAGCAGC CAGGAGCTGG GAGAAGAGGA GCCGGCGCGC 
ACCGCTGTCC CAGCGCTAGC TGCTGCTGGC CGGGGGCGTC TCTCCAGCCA GCAGCGGCAG 
TGCTGCTTCT CTGAGCCAGG CAAATTACAT CGCGTAGGTT GGAGCGGTGG GCCCACTGGT 
GAGGACTTGA GAAAAGATTA TTCCACGCAG GAGTTGCAGC GTGGGATGCA CGCAAAGTCC 
AAAGGGCTGC TGGAGACTCA GTCCCTAAGC TCAACGGAGC TGAATTCCGG GCCAGCGGAT 
CTTGGCAATG CCCATAGACC TGCGGGTCGG AGTCAGAGTG TTTCAGGCGA GGTCATGGGT 
CCCTGCAAGG GTTCAGAAAG GACTGTGGCC ACTGTCCAGG CTGTTCCTCA AAGAGCAGAC 
ATCCGCAGAC CACTGCTTCA CACCAAGCTT TCCAGGTCTT TGACTCAGAA GGAGGTCACA 
GGAGTGTGTC CTGGAGAGGC CCTCCAGACC AAACCGGCTG GCTGTGGTCG GAGAATCTCT 
GAGACCTCGG TGTCTACTCC GGGCCCCTCC CTCCCTGAAG ATGACGTGTT CCTGAGAGAA 
GCCAAAACAC CGTCACCTCA AGATTCCCAA GGGCTCCCCA CCAGCACCTC CTACCGGCAG 
TATGAAAACG ACTTAAGCAA AAAAGCTGGT CAGATTGCAG TTTCAGCAGA GAGACCCCTC 
CATGAGACTC CAGGGATCAC GGGTACAGAG GACTGTGGGC AAGCGGTGAA CGGCTCTGTG 
GATCTCTCCA GACCCACAAG CATTCCAGAG ACTACAAATG ACGACATCCC AACCTTTGAC 
ACTAATGGGA CCACTGACCC CTCTGCAGCT ACAGAGAAAA AACCCCTCAA GCCTCCCCCA 
GTCGATGTCT TGAGACCTTC AGACTCTGAG ACTCCAGGGT CCCCTCACCA CACTTCCCTG 
ACTTGGGGCC AGTTTGATTC CAAGACTACT TGGCCTAGTC GGCATTTTGA GGCGCTGGTT 
CAAGAGCTGG CCAGACTGGA TCCCTCTTTG AGCCGTACTC TTGCCGCCCA GCCCGGTCCA 
GAGCCACCGC AGGGCCTGCT GGATGGGCTT TTTCCTGTCG AAGAGATCCG AAGTGCGATG 
AGGCCAGCCC TTGAGGAGAT GGGAGAGAAG GCTGCTGGCG CATCGGAGGA AGGGTCTTGT 
GGACACCACC TCACGCAGGA CCTGCAGACT TCCCAAGAGG CATCAAGGTC TGAAAACTCT 
ACCCCCGACC CTGACCAATC AAGTGGCCAG GAATTCCCAG AAGGAAACAG CACCCAGGCC 
AAGAAAGTGG AACTAGCCCG CCTCCTCCAA AAGATGCTGC AGGATCTTCA CGCTGAGCAG 
GAGCGACTGC GGGGGACCGC CGCAGACTGG ACCCAACGCA ACGGAGCTCT AGAGGCTGCT 
GTGAGCCAGG CCTGTACACC CCGGGAGCTG GAACGGTTCC GCCGGTTCAT GACTGATCTA 
GAGCGTGTGC TTGGTCTGCT GCTGCTGCTA GGCAGTCGCC TGGCCTCTCT GCTGCAGCGA 
CTCCAACTTC TGCAGCGACA GCAAGAAGAA GCTAAGGAGC TGAAGGAGCA CGTGGCTCGG 
CGGGAGCAAA CCCTGCGTCA GGTGTTGGAG CGGGAGCTGC CCGCAGAGCA TCTGCGCTCC 
TATTGTGTGC TGCTGGCCTC CAAGGCCAGG ATCCTGTCAC AGCAGCGCAG CCTGGACGAT 
CGAATCCGGT TCCTTAAGGA CCAACTGGAC ACTATCTGGA GCGACCTCAG CCATCATCCC 
CTTTCTCCCA GACTGACCTG GGCCCCAGCA ATCCGTCCTC TAAACAAACA ACCTTTTCTT 
GCTACCCTTA TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006534322.1
CDS	282..2696
Translation	MEALGTGRDRTSQASATESLDLRRLSTRADSAYSSFSTASGDPETRTPSPGTERLPYLDWDYVRVVWGSQSPTSKDAVLSTTQRPVQAVAGHSDPRSPEVQGSPGPLNRQDTPLLYALAAEAEATAHTAEPPSPPASRDAYRQRLQGAQRRVLRETSFQRKEFRMSLPGRLRPAVPTRLPTAHVRSASSSQELGEEEPARTAVPALAAAGRGRLSSQQRQCCFSEPGKLHRVGWSGGPTGEDLRKDYSTQELQRGMHAKSKGLLETQSLSSTELNSGPADLGNAHRPAGRSQSVSGEVMGPCKGSERTVATVQAVPQRADIRRPLLHTKLSRSLTQKEVTGVCPGEALQTKPAGCGRRISETSVSTPGPSLPEDDVFLREAKTPSPQDSQGLPTSTSYRQYENDLSKKAGQIAVSAERPLHETPGITGTEDCGQAVNGSVDLSRPTSIPETTNDDIPTFDTNGTTDPSAATEKKPLKPPPVDVLRPSDSETPGSPHHTSLTWGQFDSKTTWPSRHFEALVQELARLDPSLSRTLAAQPGPEPPQGLLDGLFPVEEIRSAMRPALEEMGEKAAGASEEGSCGHHLTQDLQTSQEASRSENSTPDPDQSSGQEFPEGNSTQAKKVELARLLQKMLQDLHAEQERLRGTAADWTQRNGALEAAVSQACTPRELERFRRFMTDLERVLGLLLLLGSRLASLLQRLQLLQRQQEEAKELKEHVARREQTLRQVLERELPAEHLRSYCVLLASKARILSQQRSLDDRIRFLKDQLDTIWSDLSHHPLSPRLTWAPAIRPLNKQPFLATLI

Target ORF information:

RefSeq Version	XM_006534259.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus shroom family member 1 (Shroom1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006534259.4

ORF Insert Sequence:

ATGGAGGCCC TGGGTACTGG GAGAGACCGC ACCTCCCAGG CCTCAGCCAC TGAAAGCCTG 
GACTTGCGAC GGTTGTCCAC GCGCGCCGAC TCTGCCTACA GCTCTTTCTC CACGGCGTCT 
GGTGATCCTG AGACGCGCAC TCCGTCCCCT GGCACCGAGC GCCTCCCTTA CCTAGACTGG 
GACTACGTGC GAGTGGTTTG GGGCAGCCAA TCTCCTACCT CGAAGGACGC TGTCCTTTCA 
ACGACTCAGC GACCTGTGCA GGCAGTCGCT GGGCACAGTG ACCCACGGTC CCCAGAGGTC 
CAGGGAAGCC CGGGACCACT AAACAGACAA GACACCCCAC TGCTGTACGC GCTGGCCGCG 
GAGGCTGAAG CTACAGCTCA CACCGCAGAG CCGCCCAGCC CGCCAGCCTC GCGGGACGCC 
TACCGCCAGC GGTTGCAGGG TGCACAGCGA CGAGTGCTGC GGGAGACGTC CTTCCAGCGA 
AAGGAGTTTC GCATGAGCCT GCCGGGCCGC CTGCGTCCCG CAGTCCCCAC GCGACTTCCC 
ACGGCGCACG TGCGCTCCGC CTCCAGCAGC CAGGAGCTGG GAGAAGAGGA GCCGGCGCGC 
ACCGCTGTCC CAGCGCTAGC TGCTGCTGGC CGGGGGCGTC TCTCCAGCCA GCAGCGGCAG 
TGCTGCTTCT CTGAGCCAGG CAAATTACAT CGCGTAGGTT GGAGCGGTGG GCCCACTGGT 
GAGGACTTGA GAAAAGATTA TTCCACGCAG GAGTTGCAGC GTGGGATGCA CGCAAAGTCC 
AAAGGGCTGC TGGAGACTCA GTCCCTAAGC TCAACGGAGC TGAATTCCGG GCCAGCGGAT 
CTTGGCAATG CCCATAGACC TGCGGGTCGG AGTCAGAGTG TTTCAGGCGA GGTCATGGGT 
CCCTGCAAGG GTTCAGAAAG GACTGTGGCC ACTGTCCAGG CTGTTCCTCA AAGAGCAGAC 
ATCCGCAGAC CACTGCTTCA CACCAAGCTT TCCAGGTCTT TGACTCAGAA GGAGGTCACA 
GGAGTGTGTC CTGGAGAGGC CCTCCAGACC AAACCGGCTG GCTGTGGTCG GAGAATCTCT 
GAGACCTCGG TGTCTACTCC GGGCCCCTCC CTCCCTGAAG ATGACGTGTT CCTGAGAGAA 
GCCAAAACAC CGTCACCTCA AGATTCCCAA GGGCTCCCCA CCAGCACCTC CTACCGGCAG 
TATGAAAACG ACTTAAGCAA AAAAGCTGGT CAGATTGCAG TTTCAGCAGA GAGACCCCTC 
CATGAGACTC CAGGGATCAC GGGTACAGAG GACTGTGGGC AAGCGGTGAA CGGCTCTGTG 
GATCTCTCCA GACCCACAAG CATTCCAGAG ACTACAAATG ACGACATCCC AACCTTTGAC 
ACTAATGGGA CCACTGACCC CTCTGCAGCT ACAGAGAAAA AACCCCTCAA GCCTCCCCCA 
GTCGATGTCT TGAGACCTTC AGACTCTGAG ACTCCAGGGT CCCCTCACCA CACTTCCCTG 
ACTTGGGGCC AGTTTGATTC CAAGACTACT TGGCCTAGTC GGCATTTTGA GGCGCTGGTT 
CAAGAGCTGG CCAGACTGGA TCCCTCTTTG AGCCGTACTC TTGCCGCCCA GCCCGGTCCA 
GAGCCACCGC AGGGCCTGCT GGATGGGCTT TTTCCTGTCG AAGAGATCCG AAGTGCGATG 
AGGCCAGCCC TTGAGGAGAT GGGAGAGAAG GCTGCTGGCG CATCGGAGGA AGGGTCTTGT 
GGACACCACC TCACGCAGGA CCTGCAGACT TCCCAAGAGG CATCAAGGTC TGAAAACTCT 
ACCCCCGACC CTGACCAATC AAGTGGCCAG GAATTCCCAG AAGGAAACAG CACCCAGGCC 
AAGAAAGTGG AACTAGCCCG CCTCCTCCAA AAGATGCTGC AGGATCTTCA CGCTGAGCAG 
GAGCGACTGC GGGGGACCGC CGCAGACTGG ACCCAACGCA ACGGAGCTCT AGAGGCTGCT 
GTGAGCCAGG CCTGTACACC CCGGGAGCTG GAACGGTTCC GCCGGTTCAT GACTGATCTA 
GAGCGTGTGC TTGGTCTGCT GCTGCTGCTA GGCAGTCGCC TGGCCTCTCT GCTGCAGCGA 
CTCCAACTTC TGCAGCGACA GCAAGAAGAA GCTAAGGAGC TGAAGGAGCA CGTGGCTCGG 
CGGGAGCAAA CCCTGCGTCA GGTGTTGGAG CGGGAGCTGC CCGCAGAGCA TCTGCGCTCC 
TATTGTGTGC TGCTGGCCTC CAAGGCCAGG ATCCTGTCAC AGCAGCGCAG CCTGGACGAT 
CGAATCCGGT TCCTTAAGGA CCAACTGGAC ACTATCTGGA GCGACCTCAG CCATCATCCC 
CTTTCTCCCA GACTGACCTG GGCCCCAGCA ATCCGTCCTC TAAACAAACA ACCTTTTCTT 
GCTACCCTTA TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structure of Shroom domain 2 reveals a three-segmented coiled-coil required for dimerization, Rock binding, and apical constriction.
Molecular biology of the cell23(11)2131-42(2012 Jun)
Mohan S,Rizaldy R,Das D,Bauer RJ,Heroux A,Trakselis MA,Hildebrand JD,VanDemark AP

The shroom family proteins play broad roles in the morphogenesis of thickened epithelial sheets.
Developmental dynamics : an official publication of the American Association of Anatomists238(6)1480-91(2009 Jun)
Lee C,Le MP,Wallingford JB

Shroom expression is attenuated in pulmonary arterial hypertension.
The European respiratory journal32(4)871-80(2008 Oct)
Sevilla-P?rez J,K?nigshoff M,Kwapiszewska G,Amarie OV,Seeger W,Weissmann N,Schermuly RT,Morty RE,Eickelberg O

Shroom2, a myosin-VIIa- and actin-binding protein, directly interacts with ZO-1 at tight junctions.
Journal of cell science120(Pt 16)2838-50(2007 Aug)
Etournay R,Zwaenepoel I,Perfettini I,Legrain P,Petit C,El-Amraoui A

Differential actin-dependent localization modulates the evolutionarily conserved activity of Shroom family proteins.
The Journal of biological chemistry281(29)20542-54(2006 Jul)
Dietz ML,Bernaciak TM,Vendetti F,Kielec JM,Hildebrand JD

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Shroom1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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