Polr2f cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Polr2f
Entrez Gene ID	69833
Full Name	polymerase (RNA) II (DNA directed) polypeptide F
Synonyms	1810060D16Rik,RPB6
General protein information	Preferred Names polymerase (RNA) II (DNA directed) polypeptide F Names DNA-directed RNA polymerases I, II, and III subunit RPABC2 DNA-directed RNA polymerase II subunit F RNA Pol II RNA polymerases I, II, and III subunit ABC2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 15 Map Location: 15 E1\|15 37.7 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_027231.1	NP_081507.1	DNA-directed RNA polymerases I, II, and III subunit RPABC2
NM_027231.2	NP_081507.1	DNA-directed RNA polymerases I, II, and III subunit RPABC2

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os03g0586600	NP_001050570.1
Xenopus tropicalis (tropical clawed frog)	polr2f	NP_001006888.1
Homo sapiens (human)	POLR2F	NP_068809.1
Gallus gallus (chicken)	POLR2F	NP_990065.1
Drosophila melanogaster (fruit fly)	RpII18	NP_524910.1
Oryza sativa (rice)	Os07g0463100	NP_001059582.1
Canis lupus familiaris (dog)	POLR2F	XP_531740.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005873	XP_315900.2
Arabidopsis thaliana (thale cress)	NRPB6A	NP_200007.1
Arabidopsis thaliana (thale cress)	NRPB6B	NP_178540.1
Pan troglodytes (chimpanzee)	POLR2F	XP_515126.1
	POLR2F	XP_001090989.1
Bos taurus (cattle)	POLR2F	NP_001032552.2
Rattus norvegicus (Norway rat)	Polr2f	NP_112625.1
Mus musculus (house mouse)	Polr2f	NP_081507.1
Danio rerio (zebrafish)	polr2f	NP_001002544.1
Caenorhabditis elegans (roundworm)	rpb-6	NP_496278.1

Gene Ontology
Bibliography

Related articles in PubMed

Diencephalic Size Is Restricted by a Novel Interplay Between GCN5 Acetyltransferase Activity and Retinoic Acid Signaling.
Wilde JJ, Siegenthaler JA, Dent SY, Niswander LA
The Journal of neuroscience : the official journal of the Society for Neuroscience37(10)2565-2579(2017 Mar)

Coordination of RNA Polymerase II Pausing and 3' End Processing Factor Recruitment with Alternative Polyadenylation.
Fusby B, Kim S, Erickson B, Kim H, Peterson ML, Bentley DL
Molecular and cellular biology36(2)295-303(2015 Nov)

Lsh mediated RNA polymerase II stalling at HoxC6 and HoxC8 involves DNA methylation.
Tao Y, Xi S, Briones V, Muegge K
PloS one5(2)e9163(2010 Feb)

A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58.
Yokoyama S, Ito Y, Ueno-Kudoh H, Shimizu H, Uchibe K, Albini S, Mitsuoka K, Miyaki S, Kiso M, Nagai A, Hikata T, Osada T, Fukuda N, Yamashita S, Harada D, Mezzano V, Kasai M, Puri PL, Hayashizaki Y, Okado H, Hashimoto M, Asahara H
Developmental cell17(6)836-48(2009 Dec)

Characterization of an exchangeable gene trap using pU-17 carrying a stop codon-beta geo cassette.
Taniwaki T, Haruna K, Nakamura H, Sekimoto T, Oike Y, Imaizumi T, Saito F, Muta M, Soejima Y, Utoh A, Nakagata N, Araki M, Yamamura K, Araki K
Development, growth & differentiation47(3)163-72(2005 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The results show that the sites of Pol II pausing and processing factor recruitment change depending on which poly(A) site is utilized. The extent of Pol II C-terminal domain Ser2 phosphorylation does not closely correlate with poly(A) site selection.
Title: Coordination of RNA Polymerase II Pausing and 3' End Processing Factor Recruitment with Alternative Polyadenylation.

Data suggests that DNA methylation can be compatible with Pol II binding at selected genes and Pol II stalling can act as alternate mechanism to explain transcriptional silencing associated with DNA methylation.
Title: Lsh mediated RNA polymerase II stalling at HoxC6 and HoxC8 involves DNA methylation.

The following Polr2f gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Polr2f cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu03906	NM_027231.1 Latest version!	Mus musculus polymerase (RNA) II (DNA directed) polypeptide F (Polr2f), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu83584	NM_027231.2 Latest version!	Mus musculus polymerase (RNA) II (DNA directed) polypeptide F (Polr2f), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Polr2f ( NM_027231.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_081507.1 Mus musculus polymerase (RNA) II (DNA directed) polypeptide F (Polr2f), mRNA.

Price & Availability↑

CloneID	OMu03906
Clone ID Related Accession (Same CDS sequence)	NM_027231.1
Accession Version	NM_027231.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 384bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-14
Organism	Mus musculus(House mouse)
Product	DNA-directed RNA polymerases I, II, and III subunit RPABC2
Comment

ATGTCAGACA ACGAGGACAA TTTCGACGGC GACGACTTTG ATGACGTTGA GGAGGACGAA 
GGACTTGACG ACTTGGAAAA TGCTGAGGAG GAGGGCCAGG AAAATGTCGA GATTCTCCCA 
TCTGGTGAGC GACCACAGGC CAACCAGAAG CGGATCACCA CTCCTTACAT GACCAAGTAT 
GAGCGTGCCC GAGTGCTGGG CACCCGGGCT CTTCAGATCG CGATGTGTGC CCCGGTGATG 
GTGGAGCTGG AGGGGGAGAC AGACCCTTTG CTCATCGCCA TGAAGGAACT CAAGGCGCGG 
AAGATCCCCA TCATCATTCG CCGGTACCTG CCAGACGGCA GCTATGAGGA CTGGGGCGTG 
GACGAGCTTA TCATCAGCGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_081507.1
Translation	MSDNEDNFDGDDFDDVEEDEGLDDLENAEEEGQENVEILPSGERPQANQKRITTPYMTKYERARVLGTRALQIAMCAPVMVELEGETDPLLIAMKELKARKIPIIIRRYLPDGSYEDWGVDELIISD

Target ORF information:

RefSeq Version	NM_027231.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus polymerase (RNA) II (DNA directed) polypeptide F (Polr2f), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_027231.1

ORF Insert Sequence:

ATGTCAGACA ACGAGGACAA TTTCGACGGC GACGACTTTG ATGACGTTGA GGAGGACGAA 
GGACTTGACG ACTTGGAAAA TGCTGAGGAG GAGGGCCAGG AAAATGTCGA GATTCTCCCA 
TCTGGTGAGC GACCACAGGC CAACCAGAAG CGGATCACCA CTCCTTACAT GACCAAGTAT 
GAGCGTGCCC GAGTGCTGGG CACCCGGGCT CTTCAGATCG CGATGTGTGC CCCGGTGATG 
GTGGAGCTGG AGGGGGAGAC AGACCCTTTG CTCATCGCCA TGAAGGAACT CAAGGCGCGG 
AAGATCCCCA TCATCATTCG CCGGTACCTG CCAGACGGCA GCTATGAGGA CTGGGGCGTG 
GACGAGCTTA TCATCAGCGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Polr2f ( NM_027231.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_081507.1 Mus musculus polymerase (RNA) II (DNA directed) polypeptide F (Polr2f), mRNA.

Price & Availability↑

CloneID	OMu83584
Clone ID Related Accession (Same CDS sequence)	NM_027231.2
Accession Version	NM_027231.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 384bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	DNA-directed RNA polymerases I, II, and III subunit RPABC2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY067308.1, AK007913.1 and AV576955.1. On Dec 8, 2015 this sequence version replaced NM_027231.1. ##Evidence-Data-START## Transcript exon combination :: AK169434.1, BU847374.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCAGACA ACGAGGACAA TTTCGACGGC GACGACTTTG ATGACGTTGA GGAGGACGAA 
GGACTTGACG ACTTGGAAAA TGCTGAGGAG GAGGGCCAGG AAAATGTCGA GATTCTCCCA 
TCTGGTGAGC GACCACAGGC CAACCAGAAG CGGATCACCA CTCCTTACAT GACCAAGTAT 
GAGCGGGCCC GGGTGCTGGG CACCCGGGCT CTTCAGATCG CGATGTGTGC CCCGGTGATG 
GTGGAGCTGG AGGGGGAGAC AGACCCTTTG CTCATCGCCA TGAAGGAACT CAAGGCGCGG 
AAGATCCCCA TCATCATTCG CCGGTACCTG CCAGACGGCA GCTATGAGGA CTGGGGCGTG 
GACGAGCTTA TCATCAGCGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_081507.1
CDS	91..474
Translation	MSDNEDNFDGDDFDDVEEDEGLDDLENAEEEGQENVEILPSGERPQANQKRITTPYMTKYERARVLGTRALQIAMCAPVMVELEGETDPLLIAMKELKARKIPIIIRRYLPDGSYEDWGVDELIISD

Target ORF information:

RefSeq Version	NM_027231.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus polymerase (RNA) II (DNA directed) polypeptide F (Polr2f), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_027231.2

ORF Insert Sequence:

ATGTCAGACA ACGAGGACAA TTTCGACGGC GACGACTTTG ATGACGTTGA GGAGGACGAA 
GGACTTGACG ACTTGGAAAA TGCTGAGGAG GAGGGCCAGG AAAATGTCGA GATTCTCCCA 
TCTGGTGAGC GACCACAGGC CAACCAGAAG CGGATCACCA CTCCTTACAT GACCAAGTAT 
GAGCGGGCCC GGGTGCTGGG CACCCGGGCT CTTCAGATCG CGATGTGTGC CCCGGTGATG 
GTGGAGCTGG AGGGGGAGAC AGACCCTTTG CTCATCGCCA TGAAGGAACT CAAGGCGCGG 
AAGATCCCCA TCATCATTCG CCGGTACCTG CCAGACGGCA GCTATGAGGA CTGGGGCGTG 
GACGAGCTTA TCATCAGCGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Diencephalic Size Is Restricted by a Novel Interplay Between GCN5 Acetyltransferase Activity and Retinoic Acid Signaling.
The Journal of neuroscience : the official journal of the Society for Neuroscience37(10)2565-2579(2017 Mar)
Wilde JJ,Siegenthaler JA,Dent SY,Niswander LA

Coordination of RNA Polymerase II Pausing and 3' End Processing Factor Recruitment with Alternative Polyadenylation.
Molecular and cellular biology36(2)295-303(2015 Nov)
Fusby B,Kim S,Erickson B,Kim H,Peterson ML,Bentley DL

Lsh mediated RNA polymerase II stalling at HoxC6 and HoxC8 involves DNA methylation.
PloS one5(2)e9163(2010 Feb)
Tao Y,Xi S,Briones V,Muegge K

A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58.
Developmental cell17(6)836-48(2009 Dec)
Yokoyama S,Ito Y,Ueno-Kudoh H,Shimizu H,Uchibe K,Albini S,Mitsuoka K,Miyaki S,Kiso M,Nagai A,Hikata T,Osada T,Fukuda N,Yamashita S,Harada D,Mezzano V,Kasai M,Puri PL,Hayashizaki Y,Okado H,Hashimoto M,Asahara H

Characterization of an exchangeable gene trap using pU-17 carrying a stop codon-beta geo cassette.
Development, growth & differentiation47(3)163-72(2005 Apr)
Taniwaki T,Haruna K,Nakamura H,Sekimoto T,Oike Y,Imaizumi T,Saito F,Muta M,Soejima Y,Utoh A,Nakagata N,Araki M,Yamamura K,Araki K

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Polr2f cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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