Cad cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cad
Entrez Gene ID	69719
Full Name	carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase
Synonyms	2410008J01Rik,AU018859,Cpad
General protein information	Preferred Names carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase Names CAD protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 B1

mRNA and Protein(s)

mRNA	Protein	Name
XM_006504092.3	XP_006504155.1	CAD protein isoform X2
XM_006504091.3	XP_006504154.1	CAD protein isoform X1
NM_001289523.1	NP_001276452.1	CAD protein isoform 3
NM_001289522.1	NP_001276451.1	CAD protein isoform 2
NM_023525.2	NP_076014.1	CAD protein isoform 1
XM_006504091.4	XP_006504154.1	CAD protein isoform X1
XM_006504092.4	XP_006504155.1	CAD protein isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CAD	XP_001155357.1
Xenopus tropicalis (tropical clawed frog)	cad	XP_004920257.1
Canis lupus familiaris (dog)	CAD	XP_540124.3
Bos taurus (cattle)	CAD	NP_001178322.1
Gallus gallus (chicken)	CAD	XP_003641116.2
Kluyveromyces lactis	KLLA0E15357g	XP_454643.1
Eremothecium gossypii	AGOS_ACR263C	NP_983665.2
Neurospora crassa	NCU08287	XP_961813.1
Danio rerio (zebrafish)	cad	NP_001009884.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000300	XP_310823.5
Caenorhabditis elegans (roundworm)	pyr-1	NP_495838.2
Saccharomyces cerevisiae (baker's yeast)	URA2	NP_012405.2
Homo sapiens (human)	CAD	NP_004332.2
Rattus norvegicus (Norway rat)	Cad	NP_001099180.2
Drosophila melanogaster (fruit fly)	r	NP_523377.1
Schizosaccharomyces pombe (fission yeast)	ura1	NP_593055.1
	CAD	XP_002799221.1
Mus musculus (house mouse)	Cad	NP_076014.1
Magnaporthe oryzae (rice blast fungus)	MGG_01537	XP_003714510.1

Gene Ontology
Bibliography

Related articles in PubMed

Novel role for caspase-activated DNase in the regulation of pathological cardiac hypertrophy.
Gao L, Huang K, Jiang DS, Liu X, Huang D, Li H, Zhang XD, Huang K
Hypertension (Dallas, Tex. : 1979)65(4)871-81(2015 Apr)

Quantitative trait loci affecting the 3D skull shape and size in mouse and prioritization of candidate genes in-silico.
Maga AM, Navarro N, Cunningham ML, Cox TC
Frontiers in physiology692(2015)

Miller (Genee-Wiedemann) syndrome represents a clinically and biochemically distinct subgroup of postaxial acrofacial dysostosis associated with partial deficiency of DHODH.
Rainger J, Bengani H, Campbell L, Anderson E, Sokhi K, Lam W, Riess A, Ansari M, Smithson S, Lees M, Mercer C, McKenzie K, Lengfeld T, Gener Querol B, Branney P, McKay S, Morrison H, Medina B, Robertson M, Kohlhase J, Gordon C, Kirk J, Wieczorek D, Fitzpatrick DR
Human molecular genetics21(18)3969-83(2012 Sep)

Autophagy suppresses tumor progression by limiting chromosomal instability.
Mathew R, Kongara S, Beaudoin B, Karp CM, Bray K, Degenhardt K, Chen G, Jin S, White E
Genes & development21(11)1367-81(2007 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CAD functions as a necessary modulator of the hypertrophic response by regulating the mitogen-activated protein kinase-extracellular signal-regulated kinase 1/2 signaling pathway in the heart.
Title: Novel role for caspase-activated DNase in the regulation of pathological cardiac hypertrophy.

The following Cad gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cad cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu14708	NM_023525.2 Latest version!	Mus musculus carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24	$1091.30 $1559.00
OMu35180	XM_006504092.4 Latest version!	Mus musculus carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu35179	XM_006504091.4 Latest version!	Mus musculus carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu14731	NM_001289523.1 Latest version!	Mus musculus carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu14732	NM_001289522.1 Latest version!	Mus musculus carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
View Old Accession Versions >>
OMu35179	XM_006504091.3	Mus musculus carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu35180	XM_006504092.3	Mus musculus carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cad ( NM_023525.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_076014.1 Mus musculus carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu14708
Clone ID Related Accession (Same CDS sequence)	NM_023525.2
Accession Version	NM_023525.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6678bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	CAD protein isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ104747.1, BC060119.1 and AC109608.14. On Jan 23, 2014 this sequence version replaced NM_023525.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: BC060119.1, BC137856.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCCC TGGTGTTGGA GGACGGGTCG GTGCTGCAGG GCCGGCCCTT TGGGGCCGCT 
GTGTCGACTG CTGGGGAAGT GGTGTTTCAG ACCGGCATGG TCGGCTACCC AGAGGCCCTC 
ACTGACCCTT CCTACAAAGC TCAGATCCTA GTGCTAACGT ATCCTCTCAT CGGCAACTAC 
GGCATTCCCT CAGATGAAGA GGACGAGTTC GGCCTGAGCA AGTGGTTTGA ATCCTCGGAG 
ATCCACGTGG CGGGACTTGT AGTGGGAGAA TGCTGTCCCA CACCCAGCCA CTGGAGTGCC 
AACTGCACCC TGCATGAGTG GCTGCAACAG CGTGGCATCC CCGGCCTGCA AGGAGTGGAC 
ACTCGGGAGC TGACTAAGAA GTTGCGGGAG CAAGGGTCTC TTTTGGGGAA GCTGGTCCAG 
AAAGGGACAG AGCCGTCAGC CCTGCCATTC GTGGACCCCA ATGCCAGGCC CCTGGCACCA 
GAGGTCTCTA TTAAGACTCC ACGGGTATTC AATGCAGGGG GTGCCCCTCG GATCTGTGCT 
CTGGATTGTG GCCTCAAGTA TAATCAGATC CGATGTCTCT GCCAGCTTGG GGCTGAAGTT 
ACCGTGGTAC CCTGGGACCA CGAGTTAGAC AGTCAGAAAT ATGACGGTCT CTTCCTAAGT 
AATGGACCCG GTGACCCTGC CTCTTACCCG GGTGTGGTGT CCACACTGAG CCGAGTCTTG 
TCTGAACCTA ACCCCCGACC TGTGTTTGGA ATCTGCCTTG GACACCAGCT GTTGGCTTTA 
GCCATCGGGG CCAAAACTTA CAAGATGAGA TACGGAAACC GAGGCCACAA CCAGCCCTGT 
TTACTGGTGG GCACTGGGCG CTGCTTTCTG ACGTCCCAGA ATCATGGGTT TGCAGTGGAC 
GCAGATTCGC TGCCAGCAGG CTGGGCTCCT CTGTTCACCA ATGCCAATGA CTGTTCCAAT 
GAAGGCATAG TGCACGACAG CCTGCCCTTC TTCAGTGTCC AGTTTCACCC AGAGCACCGG 
GCCGGCCCTT CAGATATGGA ACTGCTCTTC GATGTATTTC TAGAAACTGT GAGAGAGGCT 
GCAGCTGGGA ACATTGGGGG CCAGACAGTA AGAGAACGCT TGGCTCAGCG CCTCTGTCCC 
CCTGAGCTCC CCATTCCTGG TTCTGGGCTC CCGCCTCCAC GAAAGGTTCT AATCCTAGGC 
TCAGGGGGCC TCTCCATTGG CCAAGCTGGA GAATTTGACT ACTCGGGCTC TCAGGCAATT 
AAAGCCCTAA AGGAGGAAAA CATCCAGACA TTACTAATCA ACCCCAACAT TGCCACAGTG 
CAGACTTCTC AGGGGCTGGC AGACAAGGTC TATTTCCTTC CCATAACACT TCACTACGTA 
ACCCAGGTAA TTCGTAATGA ACGCCCAGAT GGTGTGTTAC TGACTTTTGG GGGTCAAACA 
GCCCTGAACT GCGGTGTGGA GCTGACCAAA GCTGGGGTAC TGGCTCGATA TGGGGTCCGG 
GTCTTGGGTA CACCCGTGGA GACCATTGAA CTGACTGAGG ACCGACGAGC CTTCGCTGCC 
AGGATGGCTG AGATCGGAGA GCATGTCGCC CCCAGTGAAG CGGCAAATTC TCTTGAACAG 
GCTCAGGCAG CTGCCGAGCG ACTAGGCTAC CCTGTGCTGG TGCGAGCAGC CTTTGCCCTG 
GGTGGTCTCG GTTCTGGCTT TGCTTCCACC AAAGAGGAAC TCTCAGCTCT TGTGGCTCCA 
GCTTTTGCCC ATACCAGTCA GGTGCTGATA GACAAGTCTC TGAAGGGCTG GAAGGAGATT 
GAATATGAGG TGGTGAGAGA CGCCTATGGC AACTGCGTAA CGGTATGCAA CATGGAGAAC 
TTAGACCCAC TGGGCATTCA TACTGGTGAA TCCATAGTGG TGGCTCCGAG CCAGACACTG 
AATGACAGAG AGTACCAGCT TCTGCGACGG ACAGCTATCA AGGTCACTCA GCACCTGGGC 
ATTGTCGGGG AGTGCAATGT ACAGTATGCT TTGAACCCCG AGTCTGAGCA GTATTACATC 
ATTGAAGTGA ATGCCAGGCT CTCCCGAAGC TCTGCCCTGG CCAGTAAGGC CACAGGCTAT 
CCACTAGCCT ATGTGGCAGC CAAGCTAGCA TTGGGCATTC CCCTGCCAGA GCTCAGAAAC 
TCTGTTACGG GAGGAACAGC AGCCTTTGAG CCCAGCCTGG ACTACTGCGT GGTAAAGATT 
CCTCGCTGGG ACCTCAGCAA GTTCCTACGT GTCAGTACGA AGATTGGGAG TTGCATGAAG 
AGTGTTGGCG AGGTCATGGG CATCGGGCGG TCCTTTGAGG AGGCCTTCCA AAAGGCCCTG 
CGCATGGTCG ATGAGAACTG CGTAGGCTTC GACCATACAG TAAAGCCAGT CAGCGACATG 
GAGCTGGAGA CTCCGACGGA TAAGCGGATC TTTGTGGTGG CTGCCGCTCT GTGGGCTGGC 
TACTCTGTGG AGCGCCTCTA CGAGCTCACC CGCATTGATT GCTGGTTCCT GCACCGGATG 
AAGCGCATTG TGACCCACGC CCAGTTGCTG GAACAACACC GTGGACAAGC TTTACCCCAA 
GACCTGCTGC ACCAGGCCAA GTGCCTCGGC TTCTCAGACA AGCAGATTGC CCTGGCAGTC 
CTGAGCACAG AGCTGGCTGT TCGGAAGCTG CGTCAGGAAC TGGGGATCTG CCCAGCGGTG 
AAACAGATTG ACACAGTTGC AGCTGAGTGG CCAGCCCAGA CAAATTACCT GTACCTGACA 
TACTGGGGCA ACACCCATGA CCTCGACTTT CGGGCACCTC ACGTCCTGGT CCTTGGCTCT 
GGTGTCTACC GGATTGGCTC CAGTGTTGAG TTTGATTGGT GTGCTGTGGG TTGCATCCAG 
CAGCTCCGGA AGATGGGTTA TAAGACCATC ATGGTGAACT ACAACCCAGA GACAGTCAGC 
ACCGACTATG ATATGTGTGA CCGACTCTAC TTTGATGAGA TCTCTTTTGA GGTGGTGATG 
GACATCTATG AGTTAGAGAA CCCCGAAGGT GTGATCCTGT CCATGGGTGG GCAGCTGCCC 
AACAACATGG CCATGGCTTT GCATCGGCAG CAGTGCCGAG TGCTGGGCAC CTCCCCCGAA 
GCCATCGACT CAGCCGAGAA CCGATTCAAG TTCTCCCGGC TCCTAGATAC CATCGGTATC 
AGCCAGCCTC AGTGGCGAGA GCTCAGTGAC CTTGAGTCTG CTCGCCAGTT CTGCCACACG 
GTCGGGTACC CCTGCGTAGT GCGCCCCTCC TATGTGCTCA GCGGTGCTGC TATGAATGTG 
GCGTACACCG ACGGGGACCT GGAGCGTTTC CTGAGCAGTG CGGCCGCCGT CTCTAAGGAG 
CACCCCGTAG TCATCTCCAA GTTCATTCAG GAAGCAAAGG AGATCGACGT GGACGCGGTG 
GCCTGTGACG GCATCGTGTC GGCCATTGCC ATCTCTGAGC ACGTGGAGAA TGCAGGTGTG 
CATTCAGGTG ATGCCACGCT GGTGACCCCC CCACAAGACA TCACCCCGAA AACTCTGGAG 
CGGATCAAAG CCATTGTGCA TGCTGTGGGC CAGGAGCTAC AGGTTACAGG ACCCTTCAAT 
CTGCAGCTCA TTGCCAAGGA TGACCAGCTG AAGGTTATTG AATGCAACGT GCGTGTATCT 
CGCTCTTTCC CCTTCGTGTC CAAGACACTG GGTGTTGATC TAGTAGCCTT GGCCACAAGG 
ATCATCATGG GGGAGAAAGT AGAGCCCGTG GGGCTCATGA CTGGCTCTGG AGTCGTGGGA 
GTAAAGGTAC CTCAGTTCTC CTTCTCACGC TTGGCCGGTG CCGACGTGGT ACTTGGTGTG 
GAGATGACCA GTACTGGAGA GGTGGCCGGC TTCGGAGAGA GCCGTTGTGA AGCCTACCTC 
AAAGCCATGC TCAGCACTGG CTTTAAGATC CCAGAGAAGA ACATCCTGCT GACTATTGGC 
AGCTACAAGA ACAAAAGTGA GCTGCTCCCA ACTGTGCGGT TGCTGGAGAG CCTGGGCTAC 
AGCCTCTATG CCAGCCTGGG TACGGCAGAC TTCTACACTG AGCACGGGGT CAAGGTAACA 
GCTGTGGACT GGCACTTTGA AGAGGCGGTG GATGGTGAGT GCCCACCACA ACGGAGCATC 
TTGGATCAGC TGGCTGAAAA TCACTTTGAG CTAGTGATTA ACCTGTCCAT GCGTGGGGCT 
GGGGGTCGGC GTCTTTCCTC CTTCGTTACC AAGGGCTACC GTACCCGGCG TCTGGCCGCT 
GATTTCTCTG TGCCTCTCAT CATTGACATC AAGTGCACCA AACTCTTTGT GGAGGCCCTA 
GGCCAGATCG GCCCAGCCCC TCCTTTGAAA GTGCACGTTG ACTGCATGAC CTCCCAGAAG 
CTAGTGCGCC TGCCCGGATT GATTGATGTC CACGTGCACC TTCGGGAACC AGGTGGGACA 
CACAAAGAGG ACTTTGCCTC AGGCACAGCT GCTGCCTTGG CTGGGGGTGT CACCATGGTT 
TGTGCTATGC CTAATACCCG ACCCCCCATC ATTGATGCTC CTGCTCTCGC CCTTGCCCAG 
AAGCTGGCAG AGGCTGGCGC CCGCTGTGAC TTTACCTTAT TCCTTGGAGC CTCATCTGAG 
AACGCGGGGA CTCTGGGTGC TGTGGCCGGC TCTGCAGCAG GCCTGAAGCT CTACCTCAAC 
GAGACCTTCT CTGAGCTGCG GCTGGACAGT GTGGCCCAGT GGATGGAGCA TTTTGAAACC 
TGGCCTGCCC ACCTCCCCAT TGTGGCCCAT GCAGAGCGGC AGAGTGTCGC TGCGGTCCTC 
ATGGTGGCTC AGCTGACCCA GCGTCCAGTG CACATATGTC ATGTGGCTCG GAAGGAAGAG 
ATCCTGTTGA TTAAAACTGC AAAGGCGCAG GGGCTGCCAG TGACCTGTGA GGTCGCTCCT 
CACCACCTGT TCCTAAACCG GGAAGACTTG GAGCGCCTGG GACCTGGGAA GGGAGAGGTC 
CGTCCTGAGC TTGGGTCCCG AGAGGATATG GAAGCCCTGT GGGAGAATAT GGCAGTCATC 
GACTGTTTTG CCTCAGACCA CGCTCCCCAT ACCTTGGAAG AGAAGTGTGG GCCCAAGCCT 
CCACCTGGCT TCCCGGGCCT AGAGACCATG TTGCCGCTGC TGCTGACAGC TGTGAGCGAG 
GGCCGCCTCA GTCTGGATGA CCTGCTGCAG CGATTGCACC ACAACCCTCG GCGGATCTTC 
CACCTGCCTC TCCAGGAGGA CACCTATGTA GAGGTGGATC TGGAGCATGA GTGGACAGTC 
CCCAGCCACA TGCCCTTCTC TAAGGCCCGC TGGACCCCAT TTGAAGGACA GAAGGTGAAG 
GGCACAGTGC GCCGTGTGGT CTTGCGAGGG GAGGTCGCCT ATATTGATGG ACAGGTGCTG 
GTACCCCCGG GCTATGGACA AGATGTACGG AAGTGGCCGC AGGGGGTTGT TCCCCAGCCC 
CCTCCTTCAA CTCCCGCCAC CACCGAAATC ACCACGACAC CTGAGCGACC ACGCCGAGTC 
ATCCCGGGCC TTCCTGATGG CCGCTTCCAC CTGCCACCCC GCATCCACCG GGCCTCTGAC 
CCAGGTCTGC CAGCTGAGGA ACCTAAAGAG AAGCCACCCA GGAAGGTAGT GGAACCAGAG 
CTGATGGGAA CCCCTGATGG TCCCTGCTAC CCTGCACCAC CAGTACCTAG ACAGGCATCA 
CCCCAGAACC TAGGGTCTTC TGGCCTGCTG CACCCCCAGA TGTCGCCCCT ACTGCACTCC 
CTAGTGGGCC AGCACATCCT GTCTGTCAAG CAGTTCACTA AGGATCAGAT GTCTCATCTG 
TTCAATGTGG CGCACACGCT ACGGATGATG GTACAGAAGG AGCGGAGCCT CGACATCCTG 
AAGGGGAAGG TCATGGCCTC CATGTTCTAC GAGGTGAGCA CCCGCACCAG TAGCTCCTTC 
GCAGCAGCCA TGGCCCGGCT CGGGGGCGCT GTTCTCAGCT TCTCAGAAGC TACATCCTCC 
GTCCAAAAGG GCGAGTCCCT TGCTGACTCG GTGCAGACCA TGAGTTGCTA TGCTGATGTC 
ATTGTGCTCC GACACCCTCA GCCTGGAGCC GTGGAGCTGG CAGCCAAGCA CTGTCGCAGG 
CCAGTGATCA ATGCTGGGGA TGGAGTTGGG GAGCACCCCA CACAGGCCCT GCTGGACATC 
TTCACCATTC GAGAAGAGCT GGGGACTGTC AATGGCATGA CGATCACTAT GGTCGGTGAC 
CTGAAGCATG GACGCACAGT GCACTCCCTG GCCTGCCTGC TCACCCAGTA CCGTGTGAGC 
CTACGCTACG TGGCACCTCC CAGCCTGCGC ATGCCACCCA GTGTACGGGA CTTTGTGGCC 
TCCCGAGGCA CCAAGCAGGA GGAGTTTGAG AGCATCGAGG AGGCGCTGCC CGACACAGAC 
GTGCTCTACA TGACTCGGAT CCAGAAAGAA CGATTTGGCT CTGTCCAGGA ATATGAAGCT 
TGCTTTGGTC AGTTCATCCT CACTCCTCAC ATCATGACCC GGGCCAAGAA GAAGATGGTG 
GTGATGCACC CAATGCCCCG TGTCAATGAG ATAAGTGTGG AGGTGGACTC GGACCCCCGG 
GCAGCCTACT TCCGCCAAGC TGAGAACGGC ATGTACATCC GCATGGCCCT GCTTGCCACC 
GTGCTTGGCC GTTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_076014.1
CDS	176..6853
Translation	MAALVLEDGSVLQGRPFGAAVSTAGEVVFQTGMVGYPEALTDPSYKAQILVLTYPLIGNYGIPSDEEDEFGLSKWFESSEIHVAGLVVGECCPTPSHWSANCTLHEWLQQRGIPGLQGVDTRELTKKLREQGSLLGKLVQKGTEPSALPFVDPNARPLAPEVSIKTPRVFNAGGAPRICALDCGLKYNQIRCLCQLGAEVTVVPWDHELDSQKYDGLFLSNGPGDPASYPGVVSTLSRVLSEPNPRPVFGICLGHQLLALAIGAKTYKMRYGNRGHNQPCLLVGTGRCFLTSQNHGFAVDADSLPAGWAPLFTNANDCSNEGIVHDSLPFFSVQFHPEHRAGPSDMELLFDVFLETVREAAAGNIGGQTVRERLAQRLCPPELPIPGSGLPPPRKVLILGSGGLSIGQAGEFDYSGSQAIKALKEENIQTLLINPNIATVQTSQGLADKVYFLPITLHYVTQVIRNERPDGVLLTFGGQTALNCGVELTKAGVLARYGVRVLGTPVETIELTEDRRAFAARMAEIGEHVAPSEAANSLEQAQAAAERLGYPVLVRAAFALGGLGSGFASTKEELSALVAPAFAHTSQVLIDKSLKGWKEIEYEVVRDAYGNCVTVCNMENLDPLGIHTGESIVVAPSQTLNDREYQLLRRTAIKVTQHLGIVGECNVQYALNPESEQYYIIEVNARLSRSSALASKATGYPLAYVAAKLALGIPLPELRNSVTGGTAAFEPSLDYCVVKIPRWDLSKFLRVSTKIGSCMKSVGEVMGIGRSFEEAFQKALRMVDENCVGFDHTVKPVSDMELETPTDKRIFVVAAALWAGYSVERLYELTRIDCWFLHRMKRIVTHAQLLEQHRGQALPQDLLHQAKCLGFSDKQIALAVLSTELAVRKLRQELGICPAVKQIDTVAAEWPAQTNYLYLTYWGNTHDLDFRAPHVLVLGSGVYRIGSSVEFDWCAVGCIQQLRKMGYKTIMVNYNPETVSTDYDMCDRLYFDEISFEVVMDIYELENPEGVILSMGGQLPNNMAMALHRQQCRVLGTSPEAIDSAENRFKFSRLLDTIGISQPQWRELSDLESARQFCHTVGYPCVVRPSYVLSGAAMNVAYTDGDLERFLSSAAAVSKEHPVVISKFIQEAKEIDVDAVACDGIVSAIAISEHVENAGVHSGDATLVTPPQDITPKTLERIKAIVHAVGQELQVTGPFNLQLIAKDDQLKVIECNVRVSRSFPFVSKTLGVDLVALATRIIMGEKVEPVGLMTGSGVVGVKVPQFSFSRLAGADVVLGVEMTSTGEVAGFGESRCEAYLKAMLSTGFKIPEKNILLTIGSYKNKSELLPTVRLLESLGYSLYASLGTADFYTEHGVKVTAVDWHFEEAVDGECPPQRSILDQLAENHFELVINLSMRGAGGRRLSSFVTKGYRTRRLAADFSVPLIIDIKCTKLFVEALGQIGPAPPLKVHVDCMTSQKLVRLPGLIDVHVHLREPGGTHKEDFASGTAAALAGGVTMVCAMPNTRPPIIDAPALALAQKLAEAGARCDFTLFLGASSENAGTLGAVAGSAAGLKLYLNETFSELRLDSVAQWMEHFETWPAHLPIVAHAERQSVAAVLMVAQLTQRPVHICHVARKEEILLIKTAKAQGLPVTCEVAPHHLFLNREDLERLGPGKGEVRPELGSREDMEALWENMAVIDCFASDHAPHTLEEKCGPKPPPGFPGLETMLPLLLTAVSEGRLSLDDLLQRLHHNPRRIFHLPLQEDTYVEVDLEHEWTVPSHMPFSKARWTPFEGQKVKGTVRRVVLRGEVAYIDGQVLVPPGYGQDVRKWPQGVVPQPPPSTPATTEITTTPERPRRVIPGLPDGRFHLPPRIHRASDPGLPAEEPKEKPPRKVVEPELMGTPDGPCYPAPPVPRQASPQNLGSSGLLHPQMSPLLHSLVGQHILSVKQFTKDQMSHLFNVAHTLRMMVQKERSLDILKGKVMASMFYEVSTRTSSSFAAAMARLGGAVLSFSEATSSVQKGESLADSVQTMSCYADVIVLRHPQPGAVELAAKHCRRPVINAGDGVGEHPTQALLDIFTIREELGTVNGMTITMVGDLKHGRTVHSLACLLTQYRVSLRYVAPPSLRMPPSVRDFVASRGTKQEEFESIEEALPDTDVLYMTRIQKERFGSVQEYEACFGQFILTPHIMTRAKKKMVVMHPMPRVNEISVEVDSDPRAAYFRQAENGMYIRMALLATVLGRF

Target ORF information:

RefSeq Version	NM_023525.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_023525.2

ORF Insert Sequence:

ATGGCGGCCC TGGTGTTGGA GGACGGGTCG GTGCTGCAGG GCCGGCCCTT TGGGGCCGCT 
GTGTCGACTG CTGGGGAAGT GGTGTTTCAG ACCGGCATGG TCGGCTACCC AGAGGCCCTC 
ACTGACCCTT CCTACAAAGC TCAGATCCTA GTGCTAACGT ATCCTCTCAT CGGCAACTAC 
GGCATTCCCT CAGATGAAGA GGACGAGTTC GGCCTGAGCA AGTGGTTTGA ATCCTCGGAG 
ATCCACGTGG CGGGACTTGT AGTGGGAGAA TGCTGTCCCA CACCCAGCCA CTGGAGTGCC 
AACTGCACCC TGCATGAGTG GCTGCAACAG CGTGGCATCC CCGGCCTGCA AGGAGTGGAC 
ACTCGGGAGC TGACTAAGAA GTTGCGGGAG CAAGGGTCTC TTTTGGGGAA GCTGGTCCAG 
AAAGGGACAG AGCCGTCAGC CCTGCCATTC GTGGACCCCA ATGCCAGGCC CCTGGCACCA 
GAGGTCTCTA TTAAGACTCC ACGGGTATTC AATGCAGGGG GTGCCCCTCG GATCTGTGCT 
CTGGATTGTG GCCTCAAGTA TAATCAGATC CGATGTCTCT GCCAGCTTGG GGCTGAAGTT 
ACCGTGGTAC CCTGGGACCA CGAGTTAGAC AGTCAGAAAT ATGACGGTCT CTTCCTAAGT 
AATGGACCCG GTGACCCTGC CTCTTACCCG GGTGTGGTGT CCACACTGAG CCGAGTCTTG 
TCTGAACCTA ACCCCCGACC TGTGTTTGGA ATCTGCCTTG GACACCAGCT GTTGGCTTTA 
GCCATCGGGG CCAAAACTTA CAAGATGAGA TACGGAAACC GAGGCCACAA CCAGCCCTGT 
TTACTGGTGG GCACTGGGCG CTGCTTTCTG ACGTCCCAGA ATCATGGGTT TGCAGTGGAC 
GCAGATTCGC TGCCAGCAGG CTGGGCTCCT CTGTTCACCA ATGCCAATGA CTGTTCCAAT 
GAAGGCATAG TGCACGACAG CCTGCCCTTC TTCAGTGTCC AGTTTCACCC AGAGCACCGG 
GCCGGCCCTT CAGATATGGA ACTGCTCTTC GATGTATTTC TAGAAACTGT GAGAGAGGCT 
GCAGCTGGGA ACATTGGGGG CCAGACAGTA AGAGAACGCT TGGCTCAGCG CCTCTGTCCC 
CCTGAGCTCC CCATTCCTGG TTCTGGGCTC CCGCCTCCAC GAAAGGTTCT AATCCTAGGC 
TCAGGGGGCC TCTCCATTGG CCAAGCTGGA GAATTTGACT ACTCGGGCTC TCAGGCAATT 
AAAGCCCTAA AGGAGGAAAA CATCCAGACA TTACTAATCA ACCCCAACAT TGCCACAGTG 
CAGACTTCTC AGGGGCTGGC AGACAAGGTC TATTTCCTTC CCATAACACT TCACTACGTA 
ACCCAGGTAA TTCGTAATGA ACGCCCAGAT GGTGTGTTAC TGACTTTTGG GGGTCAAACA 
GCCCTGAACT GCGGTGTGGA GCTGACCAAA GCTGGGGTAC TGGCTCGATA TGGGGTCCGG 
GTCTTGGGTA CACCCGTGGA GACCATTGAA CTGACTGAGG ACCGACGAGC CTTCGCTGCC 
AGGATGGCTG AGATCGGAGA GCATGTCGCC CCCAGTGAAG CGGCAAATTC TCTTGAACAG 
GCTCAGGCAG CTGCCGAGCG ACTAGGCTAC CCTGTGCTGG TGCGAGCAGC CTTTGCCCTG 
GGTGGTCTCG GTTCTGGCTT TGCTTCCACC AAAGAGGAAC TCTCAGCTCT TGTGGCTCCA 
GCTTTTGCCC ATACCAGTCA GGTGCTGATA GACAAGTCTC TGAAGGGCTG GAAGGAGATT 
GAATATGAGG TGGTGAGAGA CGCCTATGGC AACTGCGTAA CGGTATGCAA CATGGAGAAC 
TTAGACCCAC TGGGCATTCA TACTGGTGAA TCCATAGTGG TGGCTCCGAG CCAGACACTG 
AATGACAGAG AGTACCAGCT TCTGCGACGG ACAGCTATCA AGGTCACTCA GCACCTGGGC 
ATTGTCGGGG AGTGCAATGT ACAGTATGCT TTGAACCCCG AGTCTGAGCA GTATTACATC 
ATTGAAGTGA ATGCCAGGCT CTCCCGAAGC TCTGCCCTGG CCAGTAAGGC CACAGGCTAT 
CCACTAGCCT ATGTGGCAGC CAAGCTAGCA TTGGGCATTC CCCTGCCAGA GCTCAGAAAC 
TCTGTTACGG GAGGAACAGC AGCCTTTGAG CCCAGCCTGG ACTACTGCGT GGTAAAGATT 
CCTCGCTGGG ACCTCAGCAA GTTCCTACGT GTCAGTACGA AGATTGGGAG TTGCATGAAG 
AGTGTTGGCG AGGTCATGGG CATCGGGCGG TCCTTTGAGG AGGCCTTCCA AAAGGCCCTG 
CGCATGGTCG ATGAGAACTG CGTAGGCTTC GACCATACAG TAAAGCCAGT CAGCGACATG 
GAGCTGGAGA CTCCGACGGA TAAGCGGATC TTTGTGGTGG CTGCCGCTCT GTGGGCTGGC 
TACTCTGTGG AGCGCCTCTA CGAGCTCACC CGCATTGATT GCTGGTTCCT GCACCGGATG 
AAGCGCATTG TGACCCACGC CCAGTTGCTG GAACAACACC GTGGACAAGC TTTACCCCAA 
GACCTGCTGC ACCAGGCCAA GTGCCTCGGC TTCTCAGACA AGCAGATTGC CCTGGCAGTC 
CTGAGCACAG AGCTGGCTGT TCGGAAGCTG CGTCAGGAAC TGGGGATCTG CCCAGCGGTG 
AAACAGATTG ACACAGTTGC AGCTGAGTGG CCAGCCCAGA CAAATTACCT GTACCTGACA 
TACTGGGGCA ACACCCATGA CCTCGACTTT CGGGCACCTC ACGTCCTGGT CCTTGGCTCT 
GGTGTCTACC GGATTGGCTC CAGTGTTGAG TTTGATTGGT GTGCTGTGGG TTGCATCCAG 
CAGCTCCGGA AGATGGGTTA TAAGACCATC ATGGTGAACT ACAACCCAGA GACAGTCAGC 
ACCGACTATG ATATGTGTGA CCGACTCTAC TTTGATGAGA TCTCTTTTGA GGTGGTGATG 
GACATCTATG AGTTAGAGAA CCCCGAAGGT GTGATCCTGT CCATGGGTGG GCAGCTGCCC 
AACAACATGG CCATGGCTTT GCATCGGCAG CAGTGCCGAG TGCTGGGCAC CTCCCCCGAA 
GCCATCGACT CAGCCGAGAA CCGATTCAAG TTCTCCCGGC TCCTAGATAC CATCGGTATC 
AGCCAGCCTC AGTGGCGAGA GCTCAGTGAC CTTGAGTCTG CTCGCCAGTT CTGCCACACG 
GTCGGGTACC CCTGCGTAGT GCGCCCCTCC TATGTGCTCA GCGGTGCTGC TATGAATGTG 
GCGTACACCG ACGGGGACCT GGAGCGTTTC CTGAGCAGTG CGGCCGCCGT CTCTAAGGAG 
CACCCCGTAG TCATCTCCAA GTTCATTCAG GAAGCAAAGG AGATCGACGT GGACGCGGTG 
GCCTGTGACG GCATCGTGTC GGCCATTGCC ATCTCTGAGC ACGTGGAGAA TGCAGGTGTG 
CATTCAGGTG ATGCCACGCT GGTGACCCCC CCACAAGACA TCACCCCGAA AACTCTGGAG 
CGGATCAAAG CCATTGTGCA TGCTGTGGGC CAGGAGCTAC AGGTTACAGG ACCCTTCAAT 
CTGCAGCTCA TTGCCAAGGA TGACCAGCTG AAGGTTATTG AATGCAACGT GCGTGTATCT 
CGCTCTTTCC CCTTCGTGTC CAAGACACTG GGTGTTGATC TAGTAGCCTT GGCCACAAGG 
ATCATCATGG GGGAGAAAGT AGAGCCCGTG GGGCTCATGA CTGGCTCTGG AGTCGTGGGA 
GTAAAGGTAC CTCAGTTCTC CTTCTCACGC TTGGCCGGTG CCGACGTGGT ACTTGGTGTG 
GAGATGACCA GTACTGGAGA GGTGGCCGGC TTCGGAGAGA GCCGTTGTGA AGCCTACCTC 
AAAGCCATGC TCAGCACTGG CTTTAAGATC CCAGAGAAGA ACATCCTGCT GACTATTGGC 
AGCTACAAGA ACAAAAGTGA GCTGCTCCCA ACTGTGCGGT TGCTGGAGAG CCTGGGCTAC 
AGCCTCTATG CCAGCCTGGG TACGGCAGAC TTCTACACTG AGCACGGGGT CAAGGTAACA 
GCTGTGGACT GGCACTTTGA AGAGGCGGTG GATGGTGAGT GCCCACCACA ACGGAGCATC 
TTGGATCAGC TGGCTGAAAA TCACTTTGAG CTAGTGATTA ACCTGTCCAT GCGTGGGGCT 
GGGGGTCGGC GTCTTTCCTC CTTCGTTACC AAGGGCTACC GTACCCGGCG TCTGGCCGCT 
GATTTCTCTG TGCCTCTCAT CATTGACATC AAGTGCACCA AACTCTTTGT GGAGGCCCTA 
GGCCAGATCG GCCCAGCCCC TCCTTTGAAA GTGCACGTTG ACTGCATGAC CTCCCAGAAG 
CTAGTGCGCC TGCCCGGATT GATTGATGTC CACGTGCACC TTCGGGAACC AGGTGGGACA 
CACAAAGAGG ACTTTGCCTC AGGCACAGCT GCTGCCTTGG CTGGGGGTGT CACCATGGTT 
TGTGCTATGC CTAATACCCG ACCCCCCATC ATTGATGCTC CTGCTCTCGC CCTTGCCCAG 
AAGCTGGCAG AGGCTGGCGC CCGCTGTGAC TTTACCTTAT TCCTTGGAGC CTCATCTGAG 
AACGCGGGGA CTCTGGGTGC TGTGGCCGGC TCTGCAGCAG GCCTGAAGCT CTACCTCAAC 
GAGACCTTCT CTGAGCTGCG GCTGGACAGT GTGGCCCAGT GGATGGAGCA TTTTGAAACC 
TGGCCTGCCC ACCTCCCCAT TGTGGCCCAT GCAGAGCGGC AGAGTGTCGC TGCGGTCCTC 
ATGGTGGCTC AGCTGACCCA GCGTCCAGTG CACATATGTC ATGTGGCTCG GAAGGAAGAG 
ATCCTGTTGA TTAAAACTGC AAAGGCGCAG GGGCTGCCAG TGACCTGTGA GGTCGCTCCT 
CACCACCTGT TCCTAAACCG GGAAGACTTG GAGCGCCTGG GACCTGGGAA GGGAGAGGTC 
CGTCCTGAGC TTGGGTCCCG AGAGGATATG GAAGCCCTGT GGGAGAATAT GGCAGTCATC 
GACTGTTTTG CCTCAGACCA CGCTCCCCAT ACCTTGGAAG AGAAGTGTGG GCCCAAGCCT 
CCACCTGGCT TCCCGGGCCT AGAGACCATG TTGCCGCTGC TGCTGACAGC TGTGAGCGAG 
GGCCGCCTCA GTCTGGATGA CCTGCTGCAG CGATTGCACC ACAACCCTCG GCGGATCTTC 
CACCTGCCTC TCCAGGAGGA CACCTATGTA GAGGTGGATC TGGAGCATGA GTGGACAGTC 
CCCAGCCACA TGCCCTTCTC TAAGGCCCGC TGGACCCCAT TTGAAGGACA GAAGGTGAAG 
GGCACAGTGC GCCGTGTGGT CTTGCGAGGG GAGGTCGCCT ATATTGATGG ACAGGTGCTG 
GTACCCCCGG GCTATGGACA AGATGTACGG AAGTGGCCGC AGGGGGTTGT TCCCCAGCCC 
CCTCCTTCAA CTCCCGCCAC CACCGAAATC ACCACGACAC CTGAGCGACC ACGCCGAGTC 
ATCCCGGGCC TTCCTGATGG CCGCTTCCAC CTGCCACCCC GCATCCACCG GGCCTCTGAC 
CCAGGTCTGC CAGCTGAGGA ACCTAAAGAG AAGCCACCCA GGAAGGTAGT GGAACCAGAG 
CTGATGGGAA CCCCTGATGG TCCCTGCTAC CCTGCACCAC CAGTACCTAG ACAGGCATCA 
CCCCAGAACC TAGGGTCTTC TGGCCTGCTG CACCCCCAGA TGTCGCCCCT ACTGCACTCC 
CTAGTGGGCC AGCACATCCT GTCTGTCAAG CAGTTCACTA AGGATCAGAT GTCTCATCTG 
TTCAATGTGG CGCACACGCT ACGGATGATG GTACAGAAGG AGCGGAGCCT CGACATCCTG 
AAGGGGAAGG TCATGGCCTC CATGTTCTAC GAGGTGAGCA CCCGCACCAG TAGCTCCTTC 
GCAGCAGCCA TGGCCCGGCT CGGGGGCGCT GTTCTCAGCT TCTCAGAAGC TACATCCTCC 
GTCCAAAAGG GCGAGTCCCT TGCTGACTCG GTGCAGACCA TGAGTTGCTA TGCTGATGTC 
ATTGTGCTCC GACACCCTCA GCCTGGAGCC GTGGAGCTGG CAGCCAAGCA CTGTCGCAGG 
CCAGTGATCA ATGCTGGGGA TGGAGTTGGG GAGCACCCCA CACAGGCCCT GCTGGACATC 
TTCACCATTC GAGAAGAGCT GGGGACTGTC AATGGCATGA CGATCACTAT GGTCGGTGAC 
CTGAAGCATG GACGCACAGT GCACTCCCTG GCCTGCCTGC TCACCCAGTA CCGTGTGAGC 
CTACGCTACG TGGCACCTCC CAGCCTGCGC ATGCCACCCA GTGTACGGGA CTTTGTGGCC 
TCCCGAGGCA CCAAGCAGGA GGAGTTTGAG AGCATCGAGG AGGCGCTGCC CGACACAGAC 
GTGCTCTACA TGACTCGGAT CCAGAAAGAA CGATTTGGCT CTGTCCAGGA ATATGAAGCT 
TGCTTTGGTC AGTTCATCCT CACTCCTCAC ATCATGACCC GGGCCAAGAA GAAGATGGTG 
GTGATGCACC CAATGCCCCG TGTCAATGAG ATAAGTGTGG AGGTGGACTC GGACCCCCGG 
GCAGCCTACT TCCGCCAAGC TGAGAACGGC ATGTACATCC GCATGGCCCT GCTTGCCACC 
GTGCTTGGCC GTTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cad ( XM_006504092.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006504155.1 Mus musculus carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad), transcript variant X2, mRNA.

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CloneID	OMu35180
Clone ID Related Accession (Same CDS sequence)	XM_006504092.3 , XM_006504092.4
Accession Version	XM_006504092.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4557bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	CAD protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006504092.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCCGC CCCTGACGTG CCCGGCCCCG CCCCTGACGT TGCCGGCTGC TTGCGCCGTC 
GCAGTCGTGC TTCAGCTCAC TACGCTTAGG GCTCTGGCTT GCCGCTCCCG CCTGCTCTCC 
AGCGCCCCGC GCAGCGAGCC ACGTGGACCA ACTCCGGCGC GCGGTGTTCG CTTGGTTCCA 
GTGGGGCTCG CCGCGCCTCC CGCGTCTGCG TGCTTGCCCT GTCTCAGCTC CGACCCGATG 
GCGGCCCTGG TGTTGGAGGA CGGGTCGGTG CTGCAGGGCC GGCCCTTTGG GGCCGCTGTG 
TCGACTGCTG GGGAAGTGGT GTTTCAGACC GGCATGGTCG GCTACCCAGA GGCCCTCACT 
GACCCTTCCT ACAAAGCTCA GATCCTAGTG CTAACGTATC CTCTCATCGG CAACTACGGC 
ATTCCCTCAG ATGAAGAGGA CGAGTTCGGC CTGAGCAAGT GGTTTGAATC CTCGGAGATC 
CACGTGGCGG GACTTGTAGT GGGAGAATGC TGTCCCACAC CCAGCCACTG GAGTGCCAAC 
TGCACCCTGC ATGAGTGGCT GCAACAGCGT GGCATCCCCG GCCTGCAAGG AGTGGACACT 
CGGGAGCTGA CTAAGAAGTT GCGGGAGCAA GGGTCTCTTT TGGGGAAGCT GGTCCAGAAA 
GGGACAGAGC CGTCAGCCCT GCCATTCGTG GACCCCAATG CCAGGCCCCT GGCACCAGAG 
GTCTCTATTA AGACTCCACG GGTATTCAAT GCAGGGGGTG CCCCTCGGAT CTGTGCTCTG 
GATTGTGGCC TCAAGTATAA TCAGATCCGA TGTCTCTGCC AGCTTGGGGC TGAAGTTACC 
GTGGTACCCT GGGACCACGA GTTAGACAGT CAGAAATATG ACGGTCTCTT CCTAAGTAAT 
GGACCCGGTG ACCCTGCCTC TTACCCGGGT GTGGTGTCCA CACTGAGCCG AGTCTTGTCT 
GAACCTAACC CCCGACCTGT GTTTGGAATC TGCCTTGGAC ACCAGCTGTT GGCTTTAGCC 
ATCGGGGCCA AAACTTACAA GATGAGATAC GGAAACCGAG GCCACAACCA GCCCTGTTTA 
CTGGTGGGCA CTGGGCGCTG CTTTCTGACG TCCCAGAATC ATGGGTTTGC AGTGGACGCA 
GATTCGCTGC CAGCAGGCTG GGCTCCTCTG TTCACCAATG CCAATGACTG TTCCAATGAA 
GGCATAGTGC ACGACAGCCT GCCCTTCTTC AGTGTCCAGT TTCACCCAGA GCACCGGGCC 
GGCCCTTCAG ATATGGAACT GCTCTTCGAT GTATTTCTAG AAACTGTGAG AGAGGCTGCA 
GCTGGGAACA TTGGGGGCCA GACAGTAAGA GAACGCTTGG CTCAGCGCCT CTGTCCCCCT 
GAGCTCCCCA TTCCTGGTTC TGGGCTCCCG CCTCCACGAA AGGTTCTAAT CCTAGGCTCA 
GGGGGCCTCT CCATTGGCCA AGCTGGAGAA TTTGACTACT CGGGCTCTCA GGCAATTAAA 
GCCCTAAAGG AGGAAAACAT CCAGACATTA CTAATCAACC CCAACATTGC CACAGTGCAG 
ACTTCTCAGG GGCTGGCAGA CAAGGTCTAT TTCCTTCCCA TAACACTTCA CTACGTAACC 
CAGGTAATTC GTAATGAACG CCCAGATGGT GTGTTACTGA CTTTTGGGGG TCAAACAGCC 
CTGAACTGCG GTGTGGAGCT GACCAAAGCT GGGGTACTGG CTCGATATGG GGTCCGGGTC 
TTGGGTACAC CCGTGGAGAC CATTGAACTG ACTGAGGACC GACGAGCCTT CGCTGCCAGG 
ATGGCTGAGA TCGGAGAGCA TGTCGCCCCC AGTGAAGCGG CAAATTCTCT TGAACAGGCT 
CAGGCAGCTG CCGAGCGACT AGGCTACCCT GTGCTGGTGC GAGCAGCCTT TGCCCTGGGT 
GGTCTCGGTT CTGGCTTTGC TTCCACCAAA GAGGAACTCT CAGCTCTTGT GGCTCCAGCT 
TTTGCCCATA CCAGTCAGGT GCTGATAGAC AAGTCTCTGA AGGGCTGGAA GGAGATTGAA 
TATGAGGTGG TGAGAGACGC CTATGGCAAC TGCGTAACGG TATGCAACAT GGAGAACTTA 
GACCCACTGG GCATTCATAC TGGTGAATCC ATAGTGGTGG CTCCGAGCCA GACACTGAAT 
GACAGAGAGT ACCAGCTTCT GCGACGGACA GCTATCAAGG TCACTCAGCA CCTGGGCATT 
GTCGGGGAGT GCAATGTACA GTATGCTTTG AACCCCGAGT CTGAGCAGTA TTACATCATT 
GAAGTGAATG CCAGGCTCTC CCGAAGCTCT GCCCTGGCCA GTAAGGCCAC AGGCTATCCA 
CTAGCCTATG TGGCAGCCAA GCTAGCATTG GGCATTCCCC TGCCAGAGCT CAGAAACTCT 
GTTACGGGAG GAACAGCAGC CTTTGAGCCC AGCCTGGACT ACTGCGTGGT AAAGATTCCT 
CGCTGGGACC TCAGCAAGTT CCTACGTGTC AGTACGAAGA TTGGGAGTTG CATGAAGAGT 
GTTGGCGAGG TCATGGGCAT CGGGCGGTCC TTTGAGGAGG CCTTCCAAAA GGCCCTGCGC 
ATGGTCGATG AGAACTGCGT AGGCTTCGAC CATACAGTAA AGCCAGTCAG CGACATGGAG 
CTGGAGACTC CGACGGATAA GCGGATCTTT GTGGTGGCTG CCGCTCTGTG GGCTGGCTAC 
TCTGTGGAGC GCCTCTACGA GCTCACCCGC ATTGATTGCT GGTTCCTGCA CCGGATGAAG 
CGCATTGTGA CCCACGCCCA GTTGCTGGAA CAACACCGTG GACAAGCTTT ACCCCAAGAC 
CTGCTGCACC AGGCCAAGTG CCTCGGCTTC TCAGACAAGC AGATTGCCCT GGCAGTCCTG 
AGCACAGAGC TGGCTGTTCG GAAGCTGCGT CAGGAACTGG GGATCTGCCC AGCGGTGAAA 
CAGATTGACA CAGTTGCAGC TGAGTGGCCA GCCCAGACAA ATTACCTGTA CCTGACATAC 
TGGGGCAACA CCCATGACCT CGACTTTCGG GCACCTCACG TCCTGGTCCT TGGCTCTGGT 
GTCTACCGGA TTGGCTCCAG TGTTGAGTTT GATTGGTGTG CTGTGGGTTG CATCCAGCAG 
CTCCGGAAGA TGGGTTATAA GACCATCATG GTGAACTACA ACCCAGAGAC AGTCAGCACC 
GACTATGATA TGTGTGACCG ACTCTACTTT GATGAGATCT CTTTTGAGGT GGTGATGGAC 
ATCTATGAGT TAGAGAACCC CGAAGGTGTG ATCCTGTCCA TGGGTGGGCA GCTGCCCAAC 
AACATGGCCA TGGCTTTGCA TCGGCAGCAG TGCCGAGTGC TGGGCACCTC CCCCGAAGCC 
ATCGACTCAG CCGAGAACCG ATTCAAGTTC TCCCGGCTCC TAGATACCAT CGGTATCAGC 
CAGCCTCAGT GGCGAGAGCT CAGTGACCTT GAGTCTGCTC GCCAGTTCTG CCACACGGTC 
GGGTACCCCT GCGTAGTGCG CCCCTCCTAT GTGCTCAGCG GTGCTGCTAT GAATGTGGCG 
TACACCGACG GGGACCTGGA GCGTTTCCTG AGCAGTGCGG CCGCCGTCTC TAAGGAGCAC 
CCCGTAGTCA TCTCCAAGTT CATTCAGGAA GCAAAGGAGA TCGACGTGGA CGCGGTGGCC 
TGTGACGGCA TCGTGTCGGC CATTGCCATC TCTGAGCACG TGGAGAATGC AGGTGTGCAT 
TCAGGTGATG CCACGCTGGT GACCCCCCCA CAAGACATCA CCCCGAAAAC TCTGGAGCGG 
ATCAAAGCCA TTGTGCATGC TGTGGGCCAG GAGCTACAGG TTACAGGACC CTTCAATCTG 
CAGCTCATTG CCAAGGATGA CCAGCTGAAG GTTATTGAAT GCAACGTGCG TGTATCTCGC 
TCTTTCCCCT TCGTGTCCAA GACACTGGGT GTTGATCTAG TAGCCTTGGC CACAAGGATC 
ATCATGGGGG AGAAAGTAGA GCCCGTGGGG CTCATGACTG GCTCTGGAGT CGTGGGAGTA 
AAGGTACCTC AGTTCTCCTT CTCACGCTTG GCCGGTGCCG ACGTGGTACT TGGTGTGGAG 
ATGACCAGTA CTGGAGAGGT GGCCGGCTTC GGAGAGAGCC GTTGTGAAGC CTACCTCAAA 
GCCATGCTCA GCACTGGCTT TAAGATCCCA GAGAAGAACA TCCTGCTGAC TATTGGCAGC 
TACAAGAACA AAAGTGAGCT GCTCCCAACT GTGCGGTTGC TGGAGAGCCT GGGCTACAGC 
CTCTATGCCA GCCTGGGTAC GGCAGACTTC TACACTGAGC ACGGGGTCAA GGTAACAGCT 
GTGGACTGGC ACTTTGAAGA GGCGGTGGAT GGTGAGTGCC CACCACAACG GAGCATCTTG 
GATCAGCTGG CTGAAAATCA CTTTGAGCTA GTGATTAACC TGTCCATGCG TGGGGCTGGG 
GGTCGGCGTC TTTCCTCCTT CGTTACCAAG GGCTACCGTA CCCGGCGTCT GGCCGCTGAT 
TTCTCTGTGC CTCTCATCAT TGACATCAAG TGCACCAAAC TCTTTGTGGA GGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006504155.1
CDS	99..4655
Translation	MVPPLTCPAPPLTLPAACAVAVVLQLTTLRALACRSRLLSSAPRSEPRGPTPARGVRLVPVGLAAPPASACLPCLSSDPMAALVLEDGSVLQGRPFGAAVSTAGEVVFQTGMVGYPEALTDPSYKAQILVLTYPLIGNYGIPSDEEDEFGLSKWFESSEIHVAGLVVGECCPTPSHWSANCTLHEWLQQRGIPGLQGVDTRELTKKLREQGSLLGKLVQKGTEPSALPFVDPNARPLAPEVSIKTPRVFNAGGAPRICALDCGLKYNQIRCLCQLGAEVTVVPWDHELDSQKYDGLFLSNGPGDPASYPGVVSTLSRVLSEPNPRPVFGICLGHQLLALAIGAKTYKMRYGNRGHNQPCLLVGTGRCFLTSQNHGFAVDADSLPAGWAPLFTNANDCSNEGIVHDSLPFFSVQFHPEHRAGPSDMELLFDVFLETVREAAAGNIGGQTVRERLAQRLCPPELPIPGSGLPPPRKVLILGSGGLSIGQAGEFDYSGSQAIKALKEENIQTLLINPNIATVQTSQGLADKVYFLPITLHYVTQVIRNERPDGVLLTFGGQTALNCGVELTKAGVLARYGVRVLGTPVETIELTEDRRAFAARMAEIGEHVAPSEAANSLEQAQAAAERLGYPVLVRAAFALGGLGSGFASTKEELSALVAPAFAHTSQVLIDKSLKGWKEIEYEVVRDAYGNCVTVCNMENLDPLGIHTGESIVVAPSQTLNDREYQLLRRTAIKVTQHLGIVGECNVQYALNPESEQYYIIEVNARLSRSSALASKATGYPLAYVAAKLALGIPLPELRNSVTGGTAAFEPSLDYCVVKIPRWDLSKFLRVSTKIGSCMKSVGEVMGIGRSFEEAFQKALRMVDENCVGFDHTVKPVSDMELETPTDKRIFVVAAALWAGYSVERLYELTRIDCWFLHRMKRIVTHAQLLEQHRGQALPQDLLHQAKCLGFSDKQIALAVLSTELAVRKLRQELGICPAVKQIDTVAAEWPAQTNYLYLTYWGNTHDLDFRAPHVLVLGSGVYRIGSSVEFDWCAVGCIQQLRKMGYKTIMVNYNPETVSTDYDMCDRLYFDEISFEVVMDIYELENPEGVILSMGGQLPNNMAMALHRQQCRVLGTSPEAIDSAENRFKFSRLLDTIGISQPQWRELSDLESARQFCHTVGYPCVVRPSYVLSGAAMNVAYTDGDLERFLSSAAAVSKEHPVVISKFIQEAKEIDVDAVACDGIVSAIAISEHVENAGVHSGDATLVTPPQDITPKTLERIKAIVHAVGQELQVTGPFNLQLIAKDDQLKVIECNVRVSRSFPFVSKTLGVDLVALATRIIMGEKVEPVGLMTGSGVVGVKVPQFSFSRLAGADVVLGVEMTSTGEVAGFGESRCEAYLKAMLSTGFKIPEKNILLTIGSYKNKSELLPTVRLLESLGYSLYASLGTADFYTEHGVKVTAVDWHFEEAVDGECPPQRSILDQLAENHFELVINLSMRGAGGRRLSSFVTKGYRTRRLAADFSVPLIIDIKCTKLFVED

Target ORF information:

RefSeq Version	XM_006504092.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006504092.4

ORF Insert Sequence:

ATGGTCCCGC CCCTGACGTG CCCGGCCCCG CCCCTGACGT TGCCGGCTGC TTGCGCCGTC 
GCAGTCGTGC TTCAGCTCAC TACGCTTAGG GCTCTGGCTT GCCGCTCCCG CCTGCTCTCC 
AGCGCCCCGC GCAGCGAGCC ACGTGGACCA ACTCCGGCGC GCGGTGTTCG CTTGGTTCCA 
GTGGGGCTCG CCGCGCCTCC CGCGTCTGCG TGCTTGCCCT GTCTCAGCTC CGACCCGATG 
GCGGCCCTGG TGTTGGAGGA CGGGTCGGTG CTGCAGGGCC GGCCCTTTGG GGCCGCTGTG 
TCGACTGCTG GGGAAGTGGT GTTTCAGACC GGCATGGTCG GCTACCCAGA GGCCCTCACT 
GACCCTTCCT ACAAAGCTCA GATCCTAGTG CTAACGTATC CTCTCATCGG CAACTACGGC 
ATTCCCTCAG ATGAAGAGGA CGAGTTCGGC CTGAGCAAGT GGTTTGAATC CTCGGAGATC 
CACGTGGCGG GACTTGTAGT GGGAGAATGC TGTCCCACAC CCAGCCACTG GAGTGCCAAC 
TGCACCCTGC ATGAGTGGCT GCAACAGCGT GGCATCCCCG GCCTGCAAGG AGTGGACACT 
CGGGAGCTGA CTAAGAAGTT GCGGGAGCAA GGGTCTCTTT TGGGGAAGCT GGTCCAGAAA 
GGGACAGAGC CGTCAGCCCT GCCATTCGTG GACCCCAATG CCAGGCCCCT GGCACCAGAG 
GTCTCTATTA AGACTCCACG GGTATTCAAT GCAGGGGGTG CCCCTCGGAT CTGTGCTCTG 
GATTGTGGCC TCAAGTATAA TCAGATCCGA TGTCTCTGCC AGCTTGGGGC TGAAGTTACC 
GTGGTACCCT GGGACCACGA GTTAGACAGT CAGAAATATG ACGGTCTCTT CCTAAGTAAT 
GGACCCGGTG ACCCTGCCTC TTACCCGGGT GTGGTGTCCA CACTGAGCCG AGTCTTGTCT 
GAACCTAACC CCCGACCTGT GTTTGGAATC TGCCTTGGAC ACCAGCTGTT GGCTTTAGCC 
ATCGGGGCCA AAACTTACAA GATGAGATAC GGAAACCGAG GCCACAACCA GCCCTGTTTA 
CTGGTGGGCA CTGGGCGCTG CTTTCTGACG TCCCAGAATC ATGGGTTTGC AGTGGACGCA 
GATTCGCTGC CAGCAGGCTG GGCTCCTCTG TTCACCAATG CCAATGACTG TTCCAATGAA 
GGCATAGTGC ACGACAGCCT GCCCTTCTTC AGTGTCCAGT TTCACCCAGA GCACCGGGCC 
GGCCCTTCAG ATATGGAACT GCTCTTCGAT GTATTTCTAG AAACTGTGAG AGAGGCTGCA 
GCTGGGAACA TTGGGGGCCA GACAGTAAGA GAACGCTTGG CTCAGCGCCT CTGTCCCCCT 
GAGCTCCCCA TTCCTGGTTC TGGGCTCCCG CCTCCACGAA AGGTTCTAAT CCTAGGCTCA 
GGGGGCCTCT CCATTGGCCA AGCTGGAGAA TTTGACTACT CGGGCTCTCA GGCAATTAAA 
GCCCTAAAGG AGGAAAACAT CCAGACATTA CTAATCAACC CCAACATTGC CACAGTGCAG 
ACTTCTCAGG GGCTGGCAGA CAAGGTCTAT TTCCTTCCCA TAACACTTCA CTACGTAACC 
CAGGTAATTC GTAATGAACG CCCAGATGGT GTGTTACTGA CTTTTGGGGG TCAAACAGCC 
CTGAACTGCG GTGTGGAGCT GACCAAAGCT GGGGTACTGG CTCGATATGG GGTCCGGGTC 
TTGGGTACAC CCGTGGAGAC CATTGAACTG ACTGAGGACC GACGAGCCTT CGCTGCCAGG 
ATGGCTGAGA TCGGAGAGCA TGTCGCCCCC AGTGAAGCGG CAAATTCTCT TGAACAGGCT 
CAGGCAGCTG CCGAGCGACT AGGCTACCCT GTGCTGGTGC GAGCAGCCTT TGCCCTGGGT 
GGTCTCGGTT CTGGCTTTGC TTCCACCAAA GAGGAACTCT CAGCTCTTGT GGCTCCAGCT 
TTTGCCCATA CCAGTCAGGT GCTGATAGAC AAGTCTCTGA AGGGCTGGAA GGAGATTGAA 
TATGAGGTGG TGAGAGACGC CTATGGCAAC TGCGTAACGG TATGCAACAT GGAGAACTTA 
GACCCACTGG GCATTCATAC TGGTGAATCC ATAGTGGTGG CTCCGAGCCA GACACTGAAT 
GACAGAGAGT ACCAGCTTCT GCGACGGACA GCTATCAAGG TCACTCAGCA CCTGGGCATT 
GTCGGGGAGT GCAATGTACA GTATGCTTTG AACCCCGAGT CTGAGCAGTA TTACATCATT 
GAAGTGAATG CCAGGCTCTC CCGAAGCTCT GCCCTGGCCA GTAAGGCCAC AGGCTATCCA 
CTAGCCTATG TGGCAGCCAA GCTAGCATTG GGCATTCCCC TGCCAGAGCT CAGAAACTCT 
GTTACGGGAG GAACAGCAGC CTTTGAGCCC AGCCTGGACT ACTGCGTGGT AAAGATTCCT 
CGCTGGGACC TCAGCAAGTT CCTACGTGTC AGTACGAAGA TTGGGAGTTG CATGAAGAGT 
GTTGGCGAGG TCATGGGCAT CGGGCGGTCC TTTGAGGAGG CCTTCCAAAA GGCCCTGCGC 
ATGGTCGATG AGAACTGCGT AGGCTTCGAC CATACAGTAA AGCCAGTCAG CGACATGGAG 
CTGGAGACTC CGACGGATAA GCGGATCTTT GTGGTGGCTG CCGCTCTGTG GGCTGGCTAC 
TCTGTGGAGC GCCTCTACGA GCTCACCCGC ATTGATTGCT GGTTCCTGCA CCGGATGAAG 
CGCATTGTGA CCCACGCCCA GTTGCTGGAA CAACACCGTG GACAAGCTTT ACCCCAAGAC 
CTGCTGCACC AGGCCAAGTG CCTCGGCTTC TCAGACAAGC AGATTGCCCT GGCAGTCCTG 
AGCACAGAGC TGGCTGTTCG GAAGCTGCGT CAGGAACTGG GGATCTGCCC AGCGGTGAAA 
CAGATTGACA CAGTTGCAGC TGAGTGGCCA GCCCAGACAA ATTACCTGTA CCTGACATAC 
TGGGGCAACA CCCATGACCT CGACTTTCGG GCACCTCACG TCCTGGTCCT TGGCTCTGGT 
GTCTACCGGA TTGGCTCCAG TGTTGAGTTT GATTGGTGTG CTGTGGGTTG CATCCAGCAG 
CTCCGGAAGA TGGGTTATAA GACCATCATG GTGAACTACA ACCCAGAGAC AGTCAGCACC 
GACTATGATA TGTGTGACCG ACTCTACTTT GATGAGATCT CTTTTGAGGT GGTGATGGAC 
ATCTATGAGT TAGAGAACCC CGAAGGTGTG ATCCTGTCCA TGGGTGGGCA GCTGCCCAAC 
AACATGGCCA TGGCTTTGCA TCGGCAGCAG TGCCGAGTGC TGGGCACCTC CCCCGAAGCC 
ATCGACTCAG CCGAGAACCG ATTCAAGTTC TCCCGGCTCC TAGATACCAT CGGTATCAGC 
CAGCCTCAGT GGCGAGAGCT CAGTGACCTT GAGTCTGCTC GCCAGTTCTG CCACACGGTC 
GGGTACCCCT GCGTAGTGCG CCCCTCCTAT GTGCTCAGCG GTGCTGCTAT GAATGTGGCG 
TACACCGACG GGGACCTGGA GCGTTTCCTG AGCAGTGCGG CCGCCGTCTC TAAGGAGCAC 
CCCGTAGTCA TCTCCAAGTT CATTCAGGAA GCAAAGGAGA TCGACGTGGA CGCGGTGGCC 
TGTGACGGCA TCGTGTCGGC CATTGCCATC TCTGAGCACG TGGAGAATGC AGGTGTGCAT 
TCAGGTGATG CCACGCTGGT GACCCCCCCA CAAGACATCA CCCCGAAAAC TCTGGAGCGG 
ATCAAAGCCA TTGTGCATGC TGTGGGCCAG GAGCTACAGG TTACAGGACC CTTCAATCTG 
CAGCTCATTG CCAAGGATGA CCAGCTGAAG GTTATTGAAT GCAACGTGCG TGTATCTCGC 
TCTTTCCCCT TCGTGTCCAA GACACTGGGT GTTGATCTAG TAGCCTTGGC CACAAGGATC 
ATCATGGGGG AGAAAGTAGA GCCCGTGGGG CTCATGACTG GCTCTGGAGT CGTGGGAGTA 
AAGGTACCTC AGTTCTCCTT CTCACGCTTG GCCGGTGCCG ACGTGGTACT TGGTGTGGAG 
ATGACCAGTA CTGGAGAGGT GGCCGGCTTC GGAGAGAGCC GTTGTGAAGC CTACCTCAAA 
GCCATGCTCA GCACTGGCTT TAAGATCCCA GAGAAGAACA TCCTGCTGAC TATTGGCAGC 
TACAAGAACA AAAGTGAGCT GCTCCCAACT GTGCGGTTGC TGGAGAGCCT GGGCTACAGC 
CTCTATGCCA GCCTGGGTAC GGCAGACTTC TACACTGAGC ACGGGGTCAA GGTAACAGCT 
GTGGACTGGC ACTTTGAAGA GGCGGTGGAT GGTGAGTGCC CACCACAACG GAGCATCTTG 
GATCAGCTGG CTGAAAATCA CTTTGAGCTA GTGATTAACC TGTCCATGCG TGGGGCTGGG 
GGTCGGCGTC TTTCCTCCTT CGTTACCAAG GGCTACCGTA CCCGGCGTCT GGCCGCTGAT 
TTCTCTGTGC CTCTCATCAT TGACATCAAG TGCACCAAAC TCTTTGTGGA GGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cad ( XM_006504092.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006504155.1 Mus musculus carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad), transcript variant X2, mRNA.

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CloneID	OMu35180
Clone ID Related Accession (Same CDS sequence)	XM_006504092.3 , XM_006504092.4
Accession Version	XM_006504092.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4557bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	CAD protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006504092.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCCGC CCCTGACGTG CCCGGCCCCG CCCCTGACGT TGCCGGCTGC TTGCGCCGTC 
GCAGTCGTGC TTCAGCTCAC TACGCTTAGG GCTCTGGCTT GCCGCTCCCG CCTGCTCTCC 
AGCGCCCCGC GCAGCGAGCC ACGTGGACCA ACTCCGGCGC GCGGTGTTCG CTTGGTTCCA 
GTGGGGCTCG CCGCGCCTCC CGCGTCTGCG TGCTTGCCCT GTCTCAGCTC CGACCCGATG 
GCGGCCCTGG TGTTGGAGGA CGGGTCGGTG CTGCAGGGCC GGCCCTTTGG GGCCGCTGTG 
TCGACTGCTG GGGAAGTGGT GTTTCAGACC GGCATGGTCG GCTACCCAGA GGCCCTCACT 
GACCCTTCCT ACAAAGCTCA GATCCTAGTG CTAACGTATC CTCTCATCGG CAACTACGGC 
ATTCCCTCAG ATGAAGAGGA CGAGTTCGGC CTGAGCAAGT GGTTTGAATC CTCGGAGATC 
CACGTGGCGG GACTTGTAGT GGGAGAATGC TGTCCCACAC CCAGCCACTG GAGTGCCAAC 
TGCACCCTGC ATGAGTGGCT GCAACAGCGT GGCATCCCCG GCCTGCAAGG AGTGGACACT 
CGGGAGCTGA CTAAGAAGTT GCGGGAGCAA GGGTCTCTTT TGGGGAAGCT GGTCCAGAAA 
GGGACAGAGC CGTCAGCCCT GCCATTCGTG GACCCCAATG CCAGGCCCCT GGCACCAGAG 
GTCTCTATTA AGACTCCACG GGTATTCAAT GCAGGGGGTG CCCCTCGGAT CTGTGCTCTG 
GATTGTGGCC TCAAGTATAA TCAGATCCGA TGTCTCTGCC AGCTTGGGGC TGAAGTTACC 
GTGGTACCCT GGGACCACGA GTTAGACAGT CAGAAATATG ACGGTCTCTT CCTAAGTAAT 
GGACCCGGTG ACCCTGCCTC TTACCCGGGT GTGGTGTCCA CACTGAGCCG AGTCTTGTCT 
GAACCTAACC CCCGACCTGT GTTTGGAATC TGCCTTGGAC ACCAGCTGTT GGCTTTAGCC 
ATCGGGGCCA AAACTTACAA GATGAGATAC GGAAACCGAG GCCACAACCA GCCCTGTTTA 
CTGGTGGGCA CTGGGCGCTG CTTTCTGACG TCCCAGAATC ATGGGTTTGC AGTGGACGCA 
GATTCGCTGC CAGCAGGCTG GGCTCCTCTG TTCACCAATG CCAATGACTG TTCCAATGAA 
GGCATAGTGC ACGACAGCCT GCCCTTCTTC AGTGTCCAGT TTCACCCAGA GCACCGGGCC 
GGCCCTTCAG ATATGGAACT GCTCTTCGAT GTATTTCTAG AAACTGTGAG AGAGGCTGCA 
GCTGGGAACA TTGGGGGCCA GACAGTAAGA GAACGCTTGG CTCAGCGCCT CTGTCCCCCT 
GAGCTCCCCA TTCCTGGTTC TGGGCTCCCG CCTCCACGAA AGGTTCTAAT CCTAGGCTCA 
GGGGGCCTCT CCATTGGCCA AGCTGGAGAA TTTGACTACT CGGGCTCTCA GGCAATTAAA 
GCCCTAAAGG AGGAAAACAT CCAGACATTA CTAATCAACC CCAACATTGC CACAGTGCAG 
ACTTCTCAGG GGCTGGCAGA CAAGGTCTAT TTCCTTCCCA TAACACTTCA CTACGTAACC 
CAGGTAATTC GTAATGAACG CCCAGATGGT GTGTTACTGA CTTTTGGGGG TCAAACAGCC 
CTGAACTGCG GTGTGGAGCT GACCAAAGCT GGGGTACTGG CTCGATATGG GGTCCGGGTC 
TTGGGTACAC CCGTGGAGAC CATTGAACTG ACTGAGGACC GACGAGCCTT CGCTGCCAGG 
ATGGCTGAGA TCGGAGAGCA TGTCGCCCCC AGTGAAGCGG CAAATTCTCT TGAACAGGCT 
CAGGCAGCTG CCGAGCGACT AGGCTACCCT GTGCTGGTGC GAGCAGCCTT TGCCCTGGGT 
GGTCTCGGTT CTGGCTTTGC TTCCACCAAA GAGGAACTCT CAGCTCTTGT GGCTCCAGCT 
TTTGCCCATA CCAGTCAGGT GCTGATAGAC AAGTCTCTGA AGGGCTGGAA GGAGATTGAA 
TATGAGGTGG TGAGAGACGC CTATGGCAAC TGCGTAACGG TATGCAACAT GGAGAACTTA 
GACCCACTGG GCATTCATAC TGGTGAATCC ATAGTGGTGG CTCCGAGCCA GACACTGAAT 
GACAGAGAGT ACCAGCTTCT GCGACGGACA GCTATCAAGG TCACTCAGCA CCTGGGCATT 
GTCGGGGAGT GCAATGTACA GTATGCTTTG AACCCCGAGT CTGAGCAGTA TTACATCATT 
GAAGTGAATG CCAGGCTCTC CCGAAGCTCT GCCCTGGCCA GTAAGGCCAC AGGCTATCCA 
CTAGCCTATG TGGCAGCCAA GCTAGCATTG GGCATTCCCC TGCCAGAGCT CAGAAACTCT 
GTTACGGGAG GAACAGCAGC CTTTGAGCCC AGCCTGGACT ACTGCGTGGT AAAGATTCCT 
CGCTGGGACC TCAGCAAGTT CCTACGTGTC AGTACGAAGA TTGGGAGTTG CATGAAGAGT 
GTTGGCGAGG TCATGGGCAT CGGGCGGTCC TTTGAGGAGG CCTTCCAAAA GGCCCTGCGC 
ATGGTCGATG AGAACTGCGT AGGCTTCGAC CATACAGTAA AGCCAGTCAG CGACATGGAG 
CTGGAGACTC CGACGGATAA GCGGATCTTT GTGGTGGCTG CCGCTCTGTG GGCTGGCTAC 
TCTGTGGAGC GCCTCTACGA GCTCACCCGC ATTGATTGCT GGTTCCTGCA CCGGATGAAG 
CGCATTGTGA CCCACGCCCA GTTGCTGGAA CAACACCGTG GACAAGCTTT ACCCCAAGAC 
CTGCTGCACC AGGCCAAGTG CCTCGGCTTC TCAGACAAGC AGATTGCCCT GGCAGTCCTG 
AGCACAGAGC TGGCTGTTCG GAAGCTGCGT CAGGAACTGG GGATCTGCCC AGCGGTGAAA 
CAGATTGACA CAGTTGCAGC TGAGTGGCCA GCCCAGACAA ATTACCTGTA CCTGACATAC 
TGGGGCAACA CCCATGACCT CGACTTTCGG GCACCTCACG TCCTGGTCCT TGGCTCTGGT 
GTCTACCGGA TTGGCTCCAG TGTTGAGTTT GATTGGTGTG CTGTGGGTTG CATCCAGCAG 
CTCCGGAAGA TGGGTTATAA GACCATCATG GTGAACTACA ACCCAGAGAC AGTCAGCACC 
GACTATGATA TGTGTGACCG ACTCTACTTT GATGAGATCT CTTTTGAGGT GGTGATGGAC 
ATCTATGAGT TAGAGAACCC CGAAGGTGTG ATCCTGTCCA TGGGTGGGCA GCTGCCCAAC 
AACATGGCCA TGGCTTTGCA TCGGCAGCAG TGCCGAGTGC TGGGCACCTC CCCCGAAGCC 
ATCGACTCAG CCGAGAACCG ATTCAAGTTC TCCCGGCTCC TAGATACCAT CGGTATCAGC 
CAGCCTCAGT GGCGAGAGCT CAGTGACCTT GAGTCTGCTC GCCAGTTCTG CCACACGGTC 
GGGTACCCCT GCGTAGTGCG CCCCTCCTAT GTGCTCAGCG GTGCTGCTAT GAATGTGGCG 
TACACCGACG GGGACCTGGA GCGTTTCCTG AGCAGTGCGG CCGCCGTCTC TAAGGAGCAC 
CCCGTAGTCA TCTCCAAGTT CATTCAGGAA GCAAAGGAGA TCGACGTGGA CGCGGTGGCC 
TGTGACGGCA TCGTGTCGGC CATTGCCATC TCTGAGCACG TGGAGAATGC AGGTGTGCAT 
TCAGGTGATG CCACGCTGGT GACCCCCCCA CAAGACATCA CCCCGAAAAC TCTGGAGCGG 
ATCAAAGCCA TTGTGCATGC TGTGGGCCAG GAGCTACAGG TTACAGGACC CTTCAATCTG 
CAGCTCATTG CCAAGGATGA CCAGCTGAAG GTTATTGAAT GCAACGTGCG TGTATCTCGC 
TCTTTCCCCT TCGTGTCCAA GACACTGGGT GTTGATCTAG TAGCCTTGGC CACAAGGATC 
ATCATGGGGG AGAAAGTAGA GCCCGTGGGG CTCATGACTG GCTCTGGAGT CGTGGGAGTA 
AAGGTACCTC AGTTCTCCTT CTCACGCTTG GCCGGTGCCG ACGTGGTACT TGGTGTGGAG 
ATGACCAGTA CTGGAGAGGT GGCCGGCTTC GGAGAGAGCC GTTGTGAAGC CTACCTCAAA 
GCCATGCTCA GCACTGGCTT TAAGATCCCA GAGAAGAACA TCCTGCTGAC TATTGGCAGC 
TACAAGAACA AAAGTGAGCT GCTCCCAACT GTGCGGTTGC TGGAGAGCCT GGGCTACAGC 
CTCTATGCCA GCCTGGGTAC GGCAGACTTC TACACTGAGC ACGGGGTCAA GGTAACAGCT 
GTGGACTGGC ACTTTGAAGA GGCGGTGGAT GGTGAGTGCC CACCACAACG GAGCATCTTG 
GATCAGCTGG CTGAAAATCA CTTTGAGCTA GTGATTAACC TGTCCATGCG TGGGGCTGGG 
GGTCGGCGTC TTTCCTCCTT CGTTACCAAG GGCTACCGTA CCCGGCGTCT GGCCGCTGAT 
TTCTCTGTGC CTCTCATCAT TGACATCAAG TGCACCAAAC TCTTTGTGGA GGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006504155.1
CDS	23..4579
Translation	MVPPLTCPAPPLTLPAACAVAVVLQLTTLRALACRSRLLSSAPRSEPRGPTPARGVRLVPVGLAAPPASACLPCLSSDPMAALVLEDGSVLQGRPFGAAVSTAGEVVFQTGMVGYPEALTDPSYKAQILVLTYPLIGNYGIPSDEEDEFGLSKWFESSEIHVAGLVVGECCPTPSHWSANCTLHEWLQQRGIPGLQGVDTRELTKKLREQGSLLGKLVQKGTEPSALPFVDPNARPLAPEVSIKTPRVFNAGGAPRICALDCGLKYNQIRCLCQLGAEVTVVPWDHELDSQKYDGLFLSNGPGDPASYPGVVSTLSRVLSEPNPRPVFGICLGHQLLALAIGAKTYKMRYGNRGHNQPCLLVGTGRCFLTSQNHGFAVDADSLPAGWAPLFTNANDCSNEGIVHDSLPFFSVQFHPEHRAGPSDMELLFDVFLETVREAAAGNIGGQTVRERLAQRLCPPELPIPGSGLPPPRKVLILGSGGLSIGQAGEFDYSGSQAIKALKEENIQTLLINPNIATVQTSQGLADKVYFLPITLHYVTQVIRNERPDGVLLTFGGQTALNCGVELTKAGVLARYGVRVLGTPVETIELTEDRRAFAARMAEIGEHVAPSEAANSLEQAQAAAERLGYPVLVRAAFALGGLGSGFASTKEELSALVAPAFAHTSQVLIDKSLKGWKEIEYEVVRDAYGNCVTVCNMENLDPLGIHTGESIVVAPSQTLNDREYQLLRRTAIKVTQHLGIVGECNVQYALNPESEQYYIIEVNARLSRSSALASKATGYPLAYVAAKLALGIPLPELRNSVTGGTAAFEPSLDYCVVKIPRWDLSKFLRVSTKIGSCMKSVGEVMGIGRSFEEAFQKALRMVDENCVGFDHTVKPVSDMELETPTDKRIFVVAAALWAGYSVERLYELTRIDCWFLHRMKRIVTHAQLLEQHRGQALPQDLLHQAKCLGFSDKQIALAVLSTELAVRKLRQELGICPAVKQIDTVAAEWPAQTNYLYLTYWGNTHDLDFRAPHVLVLGSGVYRIGSSVEFDWCAVGCIQQLRKMGYKTIMVNYNPETVSTDYDMCDRLYFDEISFEVVMDIYELENPEGVILSMGGQLPNNMAMALHRQQCRVLGTSPEAIDSAENRFKFSRLLDTIGISQPQWRELSDLESARQFCHTVGYPCVVRPSYVLSGAAMNVAYTDGDLERFLSSAAAVSKEHPVVISKFIQEAKEIDVDAVACDGIVSAIAISEHVENAGVHSGDATLVTPPQDITPKTLERIKAIVHAVGQELQVTGPFNLQLIAKDDQLKVIECNVRVSRSFPFVSKTLGVDLVALATRIIMGEKVEPVGLMTGSGVVGVKVPQFSFSRLAGADVVLGVEMTSTGEVAGFGESRCEAYLKAMLSTGFKIPEKNILLTIGSYKNKSELLPTVRLLESLGYSLYASLGTADFYTEHGVKVTAVDWHFEEAVDGECPPQRSILDQLAENHFELVINLSMRGAGGRRLSSFVTKGYRTRRLAADFSVPLIIDIKCTKLFVED

Target ORF information:

RefSeq Version	XM_006504092.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006504092.3

ORF Insert Sequence:

ATGGTCCCGC CCCTGACGTG CCCGGCCCCG CCCCTGACGT TGCCGGCTGC TTGCGCCGTC 
GCAGTCGTGC TTCAGCTCAC TACGCTTAGG GCTCTGGCTT GCCGCTCCCG CCTGCTCTCC 
AGCGCCCCGC GCAGCGAGCC ACGTGGACCA ACTCCGGCGC GCGGTGTTCG CTTGGTTCCA 
GTGGGGCTCG CCGCGCCTCC CGCGTCTGCG TGCTTGCCCT GTCTCAGCTC CGACCCGATG 
GCGGCCCTGG TGTTGGAGGA CGGGTCGGTG CTGCAGGGCC GGCCCTTTGG GGCCGCTGTG 
TCGACTGCTG GGGAAGTGGT GTTTCAGACC GGCATGGTCG GCTACCCAGA GGCCCTCACT 
GACCCTTCCT ACAAAGCTCA GATCCTAGTG CTAACGTATC CTCTCATCGG CAACTACGGC 
ATTCCCTCAG ATGAAGAGGA CGAGTTCGGC CTGAGCAAGT GGTTTGAATC CTCGGAGATC 
CACGTGGCGG GACTTGTAGT GGGAGAATGC TGTCCCACAC CCAGCCACTG GAGTGCCAAC 
TGCACCCTGC ATGAGTGGCT GCAACAGCGT GGCATCCCCG GCCTGCAAGG AGTGGACACT 
CGGGAGCTGA CTAAGAAGTT GCGGGAGCAA GGGTCTCTTT TGGGGAAGCT GGTCCAGAAA 
GGGACAGAGC CGTCAGCCCT GCCATTCGTG GACCCCAATG CCAGGCCCCT GGCACCAGAG 
GTCTCTATTA AGACTCCACG GGTATTCAAT GCAGGGGGTG CCCCTCGGAT CTGTGCTCTG 
GATTGTGGCC TCAAGTATAA TCAGATCCGA TGTCTCTGCC AGCTTGGGGC TGAAGTTACC 
GTGGTACCCT GGGACCACGA GTTAGACAGT CAGAAATATG ACGGTCTCTT CCTAAGTAAT 
GGACCCGGTG ACCCTGCCTC TTACCCGGGT GTGGTGTCCA CACTGAGCCG AGTCTTGTCT 
GAACCTAACC CCCGACCTGT GTTTGGAATC TGCCTTGGAC ACCAGCTGTT GGCTTTAGCC 
ATCGGGGCCA AAACTTACAA GATGAGATAC GGAAACCGAG GCCACAACCA GCCCTGTTTA 
CTGGTGGGCA CTGGGCGCTG CTTTCTGACG TCCCAGAATC ATGGGTTTGC AGTGGACGCA 
GATTCGCTGC CAGCAGGCTG GGCTCCTCTG TTCACCAATG CCAATGACTG TTCCAATGAA 
GGCATAGTGC ACGACAGCCT GCCCTTCTTC AGTGTCCAGT TTCACCCAGA GCACCGGGCC 
GGCCCTTCAG ATATGGAACT GCTCTTCGAT GTATTTCTAG AAACTGTGAG AGAGGCTGCA 
GCTGGGAACA TTGGGGGCCA GACAGTAAGA GAACGCTTGG CTCAGCGCCT CTGTCCCCCT 
GAGCTCCCCA TTCCTGGTTC TGGGCTCCCG CCTCCACGAA AGGTTCTAAT CCTAGGCTCA 
GGGGGCCTCT CCATTGGCCA AGCTGGAGAA TTTGACTACT CGGGCTCTCA GGCAATTAAA 
GCCCTAAAGG AGGAAAACAT CCAGACATTA CTAATCAACC CCAACATTGC CACAGTGCAG 
ACTTCTCAGG GGCTGGCAGA CAAGGTCTAT TTCCTTCCCA TAACACTTCA CTACGTAACC 
CAGGTAATTC GTAATGAACG CCCAGATGGT GTGTTACTGA CTTTTGGGGG TCAAACAGCC 
CTGAACTGCG GTGTGGAGCT GACCAAAGCT GGGGTACTGG CTCGATATGG GGTCCGGGTC 
TTGGGTACAC CCGTGGAGAC CATTGAACTG ACTGAGGACC GACGAGCCTT CGCTGCCAGG 
ATGGCTGAGA TCGGAGAGCA TGTCGCCCCC AGTGAAGCGG CAAATTCTCT TGAACAGGCT 
CAGGCAGCTG CCGAGCGACT AGGCTACCCT GTGCTGGTGC GAGCAGCCTT TGCCCTGGGT 
GGTCTCGGTT CTGGCTTTGC TTCCACCAAA GAGGAACTCT CAGCTCTTGT GGCTCCAGCT 
TTTGCCCATA CCAGTCAGGT GCTGATAGAC AAGTCTCTGA AGGGCTGGAA GGAGATTGAA 
TATGAGGTGG TGAGAGACGC CTATGGCAAC TGCGTAACGG TATGCAACAT GGAGAACTTA 
GACCCACTGG GCATTCATAC TGGTGAATCC ATAGTGGTGG CTCCGAGCCA GACACTGAAT 
GACAGAGAGT ACCAGCTTCT GCGACGGACA GCTATCAAGG TCACTCAGCA CCTGGGCATT 
GTCGGGGAGT GCAATGTACA GTATGCTTTG AACCCCGAGT CTGAGCAGTA TTACATCATT 
GAAGTGAATG CCAGGCTCTC CCGAAGCTCT GCCCTGGCCA GTAAGGCCAC AGGCTATCCA 
CTAGCCTATG TGGCAGCCAA GCTAGCATTG GGCATTCCCC TGCCAGAGCT CAGAAACTCT 
GTTACGGGAG GAACAGCAGC CTTTGAGCCC AGCCTGGACT ACTGCGTGGT AAAGATTCCT 
CGCTGGGACC TCAGCAAGTT CCTACGTGTC AGTACGAAGA TTGGGAGTTG CATGAAGAGT 
GTTGGCGAGG TCATGGGCAT CGGGCGGTCC TTTGAGGAGG CCTTCCAAAA GGCCCTGCGC 
ATGGTCGATG AGAACTGCGT AGGCTTCGAC CATACAGTAA AGCCAGTCAG CGACATGGAG 
CTGGAGACTC CGACGGATAA GCGGATCTTT GTGGTGGCTG CCGCTCTGTG GGCTGGCTAC 
TCTGTGGAGC GCCTCTACGA GCTCACCCGC ATTGATTGCT GGTTCCTGCA CCGGATGAAG 
CGCATTGTGA CCCACGCCCA GTTGCTGGAA CAACACCGTG GACAAGCTTT ACCCCAAGAC 
CTGCTGCACC AGGCCAAGTG CCTCGGCTTC TCAGACAAGC AGATTGCCCT GGCAGTCCTG 
AGCACAGAGC TGGCTGTTCG GAAGCTGCGT CAGGAACTGG GGATCTGCCC AGCGGTGAAA 
CAGATTGACA CAGTTGCAGC TGAGTGGCCA GCCCAGACAA ATTACCTGTA CCTGACATAC 
TGGGGCAACA CCCATGACCT CGACTTTCGG GCACCTCACG TCCTGGTCCT TGGCTCTGGT 
GTCTACCGGA TTGGCTCCAG TGTTGAGTTT GATTGGTGTG CTGTGGGTTG CATCCAGCAG 
CTCCGGAAGA TGGGTTATAA GACCATCATG GTGAACTACA ACCCAGAGAC AGTCAGCACC 
GACTATGATA TGTGTGACCG ACTCTACTTT GATGAGATCT CTTTTGAGGT GGTGATGGAC 
ATCTATGAGT TAGAGAACCC CGAAGGTGTG ATCCTGTCCA TGGGTGGGCA GCTGCCCAAC 
AACATGGCCA TGGCTTTGCA TCGGCAGCAG TGCCGAGTGC TGGGCACCTC CCCCGAAGCC 
ATCGACTCAG CCGAGAACCG ATTCAAGTTC TCCCGGCTCC TAGATACCAT CGGTATCAGC 
CAGCCTCAGT GGCGAGAGCT CAGTGACCTT GAGTCTGCTC GCCAGTTCTG CCACACGGTC 
GGGTACCCCT GCGTAGTGCG CCCCTCCTAT GTGCTCAGCG GTGCTGCTAT GAATGTGGCG 
TACACCGACG GGGACCTGGA GCGTTTCCTG AGCAGTGCGG CCGCCGTCTC TAAGGAGCAC 
CCCGTAGTCA TCTCCAAGTT CATTCAGGAA GCAAAGGAGA TCGACGTGGA CGCGGTGGCC 
TGTGACGGCA TCGTGTCGGC CATTGCCATC TCTGAGCACG TGGAGAATGC AGGTGTGCAT 
TCAGGTGATG CCACGCTGGT GACCCCCCCA CAAGACATCA CCCCGAAAAC TCTGGAGCGG 
ATCAAAGCCA TTGTGCATGC TGTGGGCCAG GAGCTACAGG TTACAGGACC CTTCAATCTG 
CAGCTCATTG CCAAGGATGA CCAGCTGAAG GTTATTGAAT GCAACGTGCG TGTATCTCGC 
TCTTTCCCCT TCGTGTCCAA GACACTGGGT GTTGATCTAG TAGCCTTGGC CACAAGGATC 
ATCATGGGGG AGAAAGTAGA GCCCGTGGGG CTCATGACTG GCTCTGGAGT CGTGGGAGTA 
AAGGTACCTC AGTTCTCCTT CTCACGCTTG GCCGGTGCCG ACGTGGTACT TGGTGTGGAG 
ATGACCAGTA CTGGAGAGGT GGCCGGCTTC GGAGAGAGCC GTTGTGAAGC CTACCTCAAA 
GCCATGCTCA GCACTGGCTT TAAGATCCCA GAGAAGAACA TCCTGCTGAC TATTGGCAGC 
TACAAGAACA AAAGTGAGCT GCTCCCAACT GTGCGGTTGC TGGAGAGCCT GGGCTACAGC 
CTCTATGCCA GCCTGGGTAC GGCAGACTTC TACACTGAGC ACGGGGTCAA GGTAACAGCT 
GTGGACTGGC ACTTTGAAGA GGCGGTGGAT GGTGAGTGCC CACCACAACG GAGCATCTTG 
GATCAGCTGG CTGAAAATCA CTTTGAGCTA GTGATTAACC TGTCCATGCG TGGGGCTGGG 
GGTCGGCGTC TTTCCTCCTT CGTTACCAAG GGCTACCGTA CCCGGCGTCT GGCCGCTGAT 
TTCTCTGTGC CTCTCATCAT TGACATCAAG TGCACCAAAC TCTTTGTGGA GGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cad ( XM_006504091.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006504154.1 Mus musculus carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu35179
Clone ID Related Accession (Same CDS sequence)	XM_006504091.3 , XM_006504091.4
Accession Version	XM_006504091.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6966bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	CAD protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006504091.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCCGC CCCTGACGTG CCCGGCCCCG CCCCTGACGT TGCCGGCTGC TTGCGCCGTC 
GCAGTCGTGC TTCAGCTCAC TACGCTTAGG GCTCTGGCTT GCCGCTCCCG CCTGCTCTCC 
AGCGCCCCGC GCAGCGAGCC ACGTGGACCA ACTCCGGCGC GCGGTGTTCG CTTGGTTCCA 
GTGGGGCTCG CCGCGCCTCC CGCGTCTGCG TGCTTGCCCT GTCTCAGCTC CGACCCGATG 
GCGGCCCTGG TGTTGGAGGA CGGGTCGGTG CTGCAGGGCC GGCCCTTTGG GGCCGCTGTG 
TCGACTGCTG GGGAAGTGGT GTTTCAGACC GGCATGGTCG GCTACCCAGA GGCCCTCACT 
GACCCTTCCT ACAAAGCTCA GATCCTAGTG CTAACGTATC CTCTCATCGG CAACTACGGC 
ATTCCCTCAG ATGAAGAGGA CGAGTTCGGC CTGAGCAAGT GGTTTGAATC CTCGGAGATC 
CACGTGGCGG GACTTGTAGT GGGAGAATGC TGTCCCACAC CCAGCCACTG GAGTGCCAAC 
TGCACCCTGC ATGAGTGGCT GCAACAGCGT GGCATCCCCG GCCTGCAAGG AGTGGACACT 
CGGGAGCTGA CTAAGAAGTT GCGGGAGCAA GGGTCTCTTT TGGGGAAGCT GGTCCAGAAA 
GGGACAGAGC CGTCAGCCCT GCCATTCGTG GACCCCAATG CCAGGCCCCT GGCACCAGAG 
GTCTCTATTA AGACTCCACG GGTATTCAAT GCAGGGGGTG CCCCTCGGAT CTGTGCTCTG 
GATTGTGGCC TCAAGTATAA TCAGATCCGA TGTCTCTGCC AGCTTGGGGC TGAAGTTACC 
GTGGTACCCT GGGACCACGA GTTAGACAGT CAGAAATATG ACGGTCTCTT CCTAAGTAAT 
GGACCCGGTG ACCCTGCCTC TTACCCGGGT GTGGTGTCCA CACTGAGCCG AGTCTTGTCT 
GAACCTAACC CCCGACCTGT GTTTGGAATC TGCCTTGGAC ACCAGCTGTT GGCTTTAGCC 
ATCGGGGCCA AAACTTACAA GATGAGATAC GGAAACCGAG GCCACAACCA GCCCTGTTTA 
CTGGTGGGCA CTGGGCGCTG CTTTCTGACG TCCCAGAATC ATGGGTTTGC AGTGGACGCA 
GATTCGCTGC CAGCAGGCTG GGCTCCTCTG TTCACCAATG CCAATGACTG TTCCAATGAA 
GGCATAGTGC ACGACAGCCT GCCCTTCTTC AGTGTCCAGT TTCACCCAGA GCACCGGGCC 
GGCCCTTCAG ATATGGAACT GCTCTTCGAT GTATTTCTAG AAACTGTGAG AGAGGCTGCA 
GCTGGGAACA TTGGGGGCCA GACAGTAAGA GAACGCTTGG CTCAGCGCCT CTGTCCCCCT 
GAGCTCCCCA TTCCTGGTTC TGGGCTCCCG CCTCCACGAA AGGTTCTAAT CCTAGGCTCA 
GGGGGCCTCT CCATTGGCCA AGCTGGAGAA TTTGACTACT CGGGCTCTCA GGCAATTAAA 
GCCCTAAAGG AGGAAAACAT CCAGACATTA CTAATCAACC CCAACATTGC CACAGTGCAG 
ACTTCTCAGG GGCTGGCAGA CAAGGTCTAT TTCCTTCCCA TAACACTTCA CTACGTAACC 
CAGGTAATTC GTAATGAACG CCCAGATGGT GTGTTACTGA CTTTTGGGGG TCAAACAGCC 
CTGAACTGCG GTGTGGAGCT GACCAAAGCT GGGGTACTGG CTCGATATGG GGTCCGGGTC 
TTGGGTACAC CCGTGGAGAC CATTGAACTG ACTGAGGACC GACGAGCCTT CGCTGCCAGG 
ATGGCTGAGA TCGGAGAGCA TGTCGCCCCC AGTGAAGCGG CAAATTCTCT TGAACAGGCT 
CAGGCAGCTG CCGAGCGACT AGGCTACCCT GTGCTGGTGC GAGCAGCCTT TGCCCTGGGT 
GGTCTCGGTT CTGGCTTTGC TTCCACCAAA GAGGAACTCT CAGCTCTTGT GGCTCCAGCT 
TTTGCCCATA CCAGTCAGGT GCTGATAGAC AAGTCTCTGA AGGGCTGGAA GGAGATTGAA 
TATGAGGTGG TGAGAGACGC CTATGGCAAC TGCGTAACGG TATGCAACAT GGAGAACTTA 
GACCCACTGG GCATTCATAC TGGTGAATCC ATAGTGGTGG CTCCGAGCCA GACACTGAAT 
GACAGAGAGT ACCAGCTTCT GCGACGGACA GCTATCAAGG TCACTCAGCA CCTGGGCATT 
GTCGGGGAGT GCAATGTACA GTATGCTTTG AACCCCGAGT CTGAGCAGTA TTACATCATT 
GAAGTGAATG CCAGGCTCTC CCGAAGCTCT GCCCTGGCCA GTAAGGCCAC AGGCTATCCA 
CTAGCCTATG TGGCAGCCAA GCTAGCATTG GGCATTCCCC TGCCAGAGCT CAGAAACTCT 
GTTACGGGAG GAACAGCAGC CTTTGAGCCC AGCCTGGACT ACTGCGTGGT AAAGATTCCT 
CGCTGGGACC TCAGCAAGTT CCTACGTGTC AGTACGAAGA TTGGGAGTTG CATGAAGAGT 
GTTGGCGAGG TCATGGGCAT CGGGCGGTCC TTTGAGGAGG CCTTCCAAAA GGCCCTGCGC 
ATGGTCGATG AGAACTGCGT AGGCTTCGAC CATACAGTAA AGCCAGTCAG CGACATGGAG 
CTGGAGACTC CGACGGATAA GCGGATCTTT GTGGTGGCTG CCGCTCTGTG GGCTGGCTAC 
TCTGTGGAGC GCCTCTACGA GCTCACCCGC ATTGATTGCT GGTTCCTGCA CCGGATGAAG 
CGCATTGTGA CCCACGCCCA GTTGCTGGAA CAACACCGTG GACAAGCTTT ACCCCAAGAC 
CTGCTGCACC AGGCCAAGTG CCTCGGCTTC TCAGACAAGC AGATTGCCCT GGCAGTCCTG 
AGCACAGAGC TGGCTGTTCG GAAGCTGCGT CAGGAACTGG GGATCTGCCC AGCGGTGAAA 
CAGATTGACA CAGTTGCAGC TGAGTGGCCA GCCCAGACAA ATTACCTGTA CCTGACATAC 
TGGGGCAACA CCCATGACCT CGACTTTCGG GCACCTCACG TCCTGGTCCT TGGCTCTGGT 
GTCTACCGGA TTGGCTCCAG TGTTGAGTTT GATTGGTGTG CTGTGGGTTG CATCCAGCAG 
CTCCGGAAGA TGGGTTATAA GACCATCATG GTGAACTACA ACCCAGAGAC AGTCAGCACC 
GACTATGATA TGTGTGACCG ACTCTACTTT GATGAGATCT CTTTTGAGGT GGTGATGGAC 
ATCTATGAGT TAGAGAACCC CGAAGGTGTG ATCCTGTCCA TGGGTGGGCA GCTGCCCAAC 
AACATGGCCA TGGCTTTGCA TCGGCAGCAG TGCCGAGTGC TGGGCACCTC CCCCGAAGCC 
ATCGACTCAG CCGAGAACCG ATTCAAGTTC TCCCGGCTCC TAGATACCAT CGGTATCAGC 
CAGCCTCAGT GGCGAGAGCT CAGTGACCTT GAGTCTGCTC GCCAGTTCTG CCACACGGTC 
GGGTACCCCT GCGTAGTGCG CCCCTCCTAT GTGCTCAGCG GTGCTGCTAT GAATGTGGCG 
TACACCGACG GGGACCTGGA GCGTTTCCTG AGCAGTGCGG CCGCCGTCTC TAAGGAGCAC 
CCCGTAGTCA TCTCCAAGTT CATTCAGGAA GCAAAGGAGA TCGACGTGGA CGCGGTGGCC 
TGTGACGGCA TCGTGTCGGC CATTGCCATC TCTGAGCACG TGGAGAATGC AGGTGTGCAT 
TCAGGTGATG CCACGCTGGT GACCCCCCCA CAAGACATCA CCCCGAAAAC TCTGGAGCGG 
ATCAAAGCCA TTGTGCATGC TGTGGGCCAG GAGCTACAGG TTACAGGACC CTTCAATCTG 
CAGCTCATTG CCAAGGATGA CCAGCTGAAG GTTATTGAAT GCAACGTGCG TGTATCTCGC 
TCTTTCCCCT TCGTGTCCAA GACACTGGGT GTTGATCTAG TAGCCTTGGC CACAAGGATC 
ATCATGGGGG AGAAAGTAGA GCCCGTGGGG CTCATGACTG GCTCTGGAGT CGTGGGAGTA 
AAGGTACCTC AGTTCTCCTT CTCACGCTTG GCCGGTGCCG ACGTGGTACT TGGTGTGGAG 
ATGACCAGTA CTGGAGAGGT GGCCGGCTTC GGAGAGAGCC GTTGTGAAGC CTACCTCAAA 
GCCATGCTCA GCACTGGCTT TAAGATCCCA GAGAAGAACA TCCTGCTGAC TATTGGCAGC 
TACAAGAACA AAAGTGAGCT GCTCCCAACT GTGCGGTTGC TGGAGAGCCT GGGCTACAGC 
CTCTATGCCA GCCTGGGTAC GGCAGACTTC TACACTGAGC ACGGGGTCAA GGTAACAGCT 
GTGGACTGGC ACTTTGAAGA GGCGGTGGAT GGTGAGTGCC CACCACAACG GAGCATCTTG 
GATCAGCTGG CTGAAAATCA CTTTGAGCTA GTGATTAACC TGTCCATGCG TGGGGCTGGG 
GGTCGGCGTC TTTCCTCCTT CGTTACCAAG GGCTACCGTA CCCGGCGTCT GGCCGCTGAT 
TTCTCTGTGC CTCTCATCAT TGACATCAAG TGCACCAAAC TCTTTGTGGA GGCCCTAGGC 
CAGATCGGCC CAGCCCCTCC TTTGAAAGTG CACGTTGACT GCATGACCTC CCAGAAGCTA 
GTGCGCCTGC CCGGATTGAT TGATGTCCAC GTGCACCTTC GGGAACCAGG TGGGACACAC 
AAAGAGGACT TTGCCTCAGG CACAGCTGCT GCCTTGGCTG GGGGTGTCAC CATGGTTTGT 
GCTATGCCTA ATACCCGACC CCCCATCATT GATGCTCCTG CTCTCGCCCT TGCCCAGAAG 
CTGGCAGAGG CTGGCGCCCG CTGTGACTTT ACCTTATTCC TTGGAGCCTC ATCTGAGAAC 
GCGGGGACTC TGGGTGCTGT GGCCGGCTCT GCAGCAGGCC TGAAGCTCTA CCTCAACGAG 
ACCTTCTCTG AGCTGCGGCT GGACAGTGTG GCCCAGTGGA TGGAGCATTT TGAAACCTGG 
CCTGCCCACC TCCCCATTGT GGCCCATGCA GAGCGGCAGA GTGTCGCTGC GGTCCTCATG 
GTGGCTCAGC TGACCCAGCG TCCAGTGCAC ATATGTCATG TGGCTCGGAA GGAAGAGATC 
CTGTTGATTA AAACTGCAAA GGCGCAGGGG CTGCCAGTGA CCTGTGAGGT CGCTCCTCAC 
CACCTGTTCC TAAACCGGGA AGACTTGGAG CGCCTGGGAC CTGGGAAGGG AGAGGTCCGT 
CCTGAGCTTG GGTCCCGAGA GGATATGGAA GCCCTGTGGG AGAATATGGC AGTCATCGAC 
TGTTTTGCCT CAGACCACGC TCCCCATACC TTGGAAGAGA AGTGTGGGCC CAAGCCTCCA 
CCTGGCTTCC CGGGCCTAGA GACCATGTTG CCGCTGCTGC TGACAGCTGT GAGCGAGGGC 
CGCCTCAGTC TGGATGACCT GCTGCAGCGA TTGCACCACA ACCCTCGGCG GATCTTCCAC 
CTGCCTCTCC AGGAGGACAC CTATGTAGAG GTGGATCTGG AGCATGAGTG GACAGTCCCC 
AGCCACATGC CCTTCTCTAA GGCCCGCTGG ACCCCATTTG AAGGACAGAA GGTGAAGGGC 
ACAGTGCGCC GTGTGGTCTT GCGAGGGGAG GTCGCCTATA TTGATGGACA GGTGCTGGTA 
CCCCCGGGCT ATGGACAAGA TGTACGGAAG TGGCCGCAGG GGGTTGTTCC CCAGCCCCCT 
CCTTCAACTC CCGCCACCAC CGAAATCACC ACGACACCTG AGCGACCACG CCGAGTCATC 
CCGGGCCTTC CTGATGGCCG CTTCCACCTG CCACCCCGCA TCCACCGGGC CTCTGACCCA 
GGTCTGCCAG CTCTGTACCT CCGCCCAGGA GCTGGGATCT CATGGGGCAG CAGAGCATGG 
GCTGAGGAAC CTAAAGAGAA GCCACCCAGG AAGGTAGTGG AACCAGAGCT GATGGGAACC 
CCTGATGGTC CCTGCTACCC TGCACCACCA GTACCTAGAC AGGCATCACC CCAGAACCTA 
GGGTCTTCTG GCCTGCTGCA CCCCCAGATG TCGCCCCTAC TGCACTCCCT AGTGGGCCAG 
CACATCCTGT CTGTCAAGCA GTTCACTAAG GATCAGATGT CTCATCTGTT CAATGTGGCG 
CACACGCTAC GGATGATGGT ACAGAAGGAG CGGAGCCTCG ACATCCTGAA GGGGAAGGTC 
ATGGCCTCCA TGTTCTACGA GGTGAGCACC CGCACCAGTA GCTCCTTCGC AGCAGCCATG 
GCCCGGCTCG GGGGCGCTGT TCTCAGCTTC TCAGAAGCTA CATCCTCCGT CCAAAAGGGC 
GAGTCCCTTG CTGACTCGGT GCAGACCATG AGTTGCTATG CTGATGTCAT TGTGCTCCGA 
CACCCTCAGC CTGGAGCCGT GGAGCTGGCA GCCAAGCACT GTCGCAGGCC AGTGATCAAT 
GCTGGGGATG GAGTTGGGGA GCACCCCACA CAGGCCCTGC TGGACATCTT CACCATTCGA 
GAAGAGCTGG GGACTGTCAA TGGCATGACG ATCACTATGG TCGGTGACCT GAAGCATGGA 
CGCACAGTGC ACTCCCTGGC CTGCCTGCTC ACCCAGTACC GTGTGAGCCT ACGCTACGTG 
GCACCTCCCA GCCTGCGCAT GCCACCCAGT GTACGGGACT TTGTGGCCTC CCGAGGCACC 
AAGCAGGAGG AGTTTGAGAG CATCGAGGAG GCGCTGCCCG ACACAGACGT GCTCTACATG 
ACTCGGATCC AGAAAGAACG ATTTGGCTCT GTCCAGGAAT ATGAAGCTTG CTTTGGTCAG 
TTCATCCTCA CTCCTCACAT CATGACCCGG GCCAAGAAGA AGATGGTGGT GATGCACCCA 
ATGCCCCGTG TCAATGAGAT AAGTGTGGAG GTGGACTCGG ACCCCCGGGC AGCCTACTTC 
CGCCAAGCTG AGAACGGCAT GTACATCCGC ATGGCCCTGC TTGCCACCGT GCTTGGCCGT 
TTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006504154.1
CDS	99..7064
Translation	MVPPLTCPAPPLTLPAACAVAVVLQLTTLRALACRSRLLSSAPRSEPRGPTPARGVRLVPVGLAAPPASACLPCLSSDPMAALVLEDGSVLQGRPFGAAVSTAGEVVFQTGMVGYPEALTDPSYKAQILVLTYPLIGNYGIPSDEEDEFGLSKWFESSEIHVAGLVVGECCPTPSHWSANCTLHEWLQQRGIPGLQGVDTRELTKKLREQGSLLGKLVQKGTEPSALPFVDPNARPLAPEVSIKTPRVFNAGGAPRICALDCGLKYNQIRCLCQLGAEVTVVPWDHELDSQKYDGLFLSNGPGDPASYPGVVSTLSRVLSEPNPRPVFGICLGHQLLALAIGAKTYKMRYGNRGHNQPCLLVGTGRCFLTSQNHGFAVDADSLPAGWAPLFTNANDCSNEGIVHDSLPFFSVQFHPEHRAGPSDMELLFDVFLETVREAAAGNIGGQTVRERLAQRLCPPELPIPGSGLPPPRKVLILGSGGLSIGQAGEFDYSGSQAIKALKEENIQTLLINPNIATVQTSQGLADKVYFLPITLHYVTQVIRNERPDGVLLTFGGQTALNCGVELTKAGVLARYGVRVLGTPVETIELTEDRRAFAARMAEIGEHVAPSEAANSLEQAQAAAERLGYPVLVRAAFALGGLGSGFASTKEELSALVAPAFAHTSQVLIDKSLKGWKEIEYEVVRDAYGNCVTVCNMENLDPLGIHTGESIVVAPSQTLNDREYQLLRRTAIKVTQHLGIVGECNVQYALNPESEQYYIIEVNARLSRSSALASKATGYPLAYVAAKLALGIPLPELRNSVTGGTAAFEPSLDYCVVKIPRWDLSKFLRVSTKIGSCMKSVGEVMGIGRSFEEAFQKALRMVDENCVGFDHTVKPVSDMELETPTDKRIFVVAAALWAGYSVERLYELTRIDCWFLHRMKRIVTHAQLLEQHRGQALPQDLLHQAKCLGFSDKQIALAVLSTELAVRKLRQELGICPAVKQIDTVAAEWPAQTNYLYLTYWGNTHDLDFRAPHVLVLGSGVYRIGSSVEFDWCAVGCIQQLRKMGYKTIMVNYNPETVSTDYDMCDRLYFDEISFEVVMDIYELENPEGVILSMGGQLPNNMAMALHRQQCRVLGTSPEAIDSAENRFKFSRLLDTIGISQPQWRELSDLESARQFCHTVGYPCVVRPSYVLSGAAMNVAYTDGDLERFLSSAAAVSKEHPVVISKFIQEAKEIDVDAVACDGIVSAIAISEHVENAGVHSGDATLVTPPQDITPKTLERIKAIVHAVGQELQVTGPFNLQLIAKDDQLKVIECNVRVSRSFPFVSKTLGVDLVALATRIIMGEKVEPVGLMTGSGVVGVKVPQFSFSRLAGADVVLGVEMTSTGEVAGFGESRCEAYLKAMLSTGFKIPEKNILLTIGSYKNKSELLPTVRLLESLGYSLYASLGTADFYTEHGVKVTAVDWHFEEAVDGECPPQRSILDQLAENHFELVINLSMRGAGGRRLSSFVTKGYRTRRLAADFSVPLIIDIKCTKLFVEALGQIGPAPPLKVHVDCMTSQKLVRLPGLIDVHVHLREPGGTHKEDFASGTAAALAGGVTMVCAMPNTRPPIIDAPALALAQKLAEAGARCDFTLFLGASSENAGTLGAVAGSAAGLKLYLNETFSELRLDSVAQWMEHFETWPAHLPIVAHAERQSVAAVLMVAQLTQRPVHICHVARKEEILLIKTAKAQGLPVTCEVAPHHLFLNREDLERLGPGKGEVRPELGSREDMEALWENMAVIDCFASDHAPHTLEEKCGPKPPPGFPGLETMLPLLLTAVSEGRLSLDDLLQRLHHNPRRIFHLPLQEDTYVEVDLEHEWTVPSHMPFSKARWTPFEGQKVKGTVRRVVLRGEVAYIDGQVLVPPGYGQDVRKWPQGVVPQPPPSTPATTEITTTPERPRRVIPGLPDGRFHLPPRIHRASDPGLPALYLRPGAGISWGSRAWAEEPKEKPPRKVVEPELMGTPDGPCYPAPPVPRQASPQNLGSSGLLHPQMSPLLHSLVGQHILSVKQFTKDQMSHLFNVAHTLRMMVQKERSLDILKGKVMASMFYEVSTRTSSSFAAAMARLGGAVLSFSEATSSVQKGESLADSVQTMSCYADVIVLRHPQPGAVELAAKHCRRPVINAGDGVGEHPTQALLDIFTIREELGTVNGMTITMVGDLKHGRTVHSLACLLTQYRVSLRYVAPPSLRMPPSVRDFVASRGTKQEEFESIEEALPDTDVLYMTRIQKERFGSVQEYEACFGQFILTPHIMTRAKKKMVVMHPMPRVNEISVEVDSDPRAAYFRQAENGMYIRMALLATVLGRF

Target ORF information:

RefSeq Version	XM_006504091.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006504091.4

ORF Insert Sequence:

ATGGTCCCGC CCCTGACGTG CCCGGCCCCG CCCCTGACGT TGCCGGCTGC TTGCGCCGTC 
GCAGTCGTGC TTCAGCTCAC TACGCTTAGG GCTCTGGCTT GCCGCTCCCG CCTGCTCTCC 
AGCGCCCCGC GCAGCGAGCC ACGTGGACCA ACTCCGGCGC GCGGTGTTCG CTTGGTTCCA 
GTGGGGCTCG CCGCGCCTCC CGCGTCTGCG TGCTTGCCCT GTCTCAGCTC CGACCCGATG 
GCGGCCCTGG TGTTGGAGGA CGGGTCGGTG CTGCAGGGCC GGCCCTTTGG GGCCGCTGTG 
TCGACTGCTG GGGAAGTGGT GTTTCAGACC GGCATGGTCG GCTACCCAGA GGCCCTCACT 
GACCCTTCCT ACAAAGCTCA GATCCTAGTG CTAACGTATC CTCTCATCGG CAACTACGGC 
ATTCCCTCAG ATGAAGAGGA CGAGTTCGGC CTGAGCAAGT GGTTTGAATC CTCGGAGATC 
CACGTGGCGG GACTTGTAGT GGGAGAATGC TGTCCCACAC CCAGCCACTG GAGTGCCAAC 
TGCACCCTGC ATGAGTGGCT GCAACAGCGT GGCATCCCCG GCCTGCAAGG AGTGGACACT 
CGGGAGCTGA CTAAGAAGTT GCGGGAGCAA GGGTCTCTTT TGGGGAAGCT GGTCCAGAAA 
GGGACAGAGC CGTCAGCCCT GCCATTCGTG GACCCCAATG CCAGGCCCCT GGCACCAGAG 
GTCTCTATTA AGACTCCACG GGTATTCAAT GCAGGGGGTG CCCCTCGGAT CTGTGCTCTG 
GATTGTGGCC TCAAGTATAA TCAGATCCGA TGTCTCTGCC AGCTTGGGGC TGAAGTTACC 
GTGGTACCCT GGGACCACGA GTTAGACAGT CAGAAATATG ACGGTCTCTT CCTAAGTAAT 
GGACCCGGTG ACCCTGCCTC TTACCCGGGT GTGGTGTCCA CACTGAGCCG AGTCTTGTCT 
GAACCTAACC CCCGACCTGT GTTTGGAATC TGCCTTGGAC ACCAGCTGTT GGCTTTAGCC 
ATCGGGGCCA AAACTTACAA GATGAGATAC GGAAACCGAG GCCACAACCA GCCCTGTTTA 
CTGGTGGGCA CTGGGCGCTG CTTTCTGACG TCCCAGAATC ATGGGTTTGC AGTGGACGCA 
GATTCGCTGC CAGCAGGCTG GGCTCCTCTG TTCACCAATG CCAATGACTG TTCCAATGAA 
GGCATAGTGC ACGACAGCCT GCCCTTCTTC AGTGTCCAGT TTCACCCAGA GCACCGGGCC 
GGCCCTTCAG ATATGGAACT GCTCTTCGAT GTATTTCTAG AAACTGTGAG AGAGGCTGCA 
GCTGGGAACA TTGGGGGCCA GACAGTAAGA GAACGCTTGG CTCAGCGCCT CTGTCCCCCT 
GAGCTCCCCA TTCCTGGTTC TGGGCTCCCG CCTCCACGAA AGGTTCTAAT CCTAGGCTCA 
GGGGGCCTCT CCATTGGCCA AGCTGGAGAA TTTGACTACT CGGGCTCTCA GGCAATTAAA 
GCCCTAAAGG AGGAAAACAT CCAGACATTA CTAATCAACC CCAACATTGC CACAGTGCAG 
ACTTCTCAGG GGCTGGCAGA CAAGGTCTAT TTCCTTCCCA TAACACTTCA CTACGTAACC 
CAGGTAATTC GTAATGAACG CCCAGATGGT GTGTTACTGA CTTTTGGGGG TCAAACAGCC 
CTGAACTGCG GTGTGGAGCT GACCAAAGCT GGGGTACTGG CTCGATATGG GGTCCGGGTC 
TTGGGTACAC CCGTGGAGAC CATTGAACTG ACTGAGGACC GACGAGCCTT CGCTGCCAGG 
ATGGCTGAGA TCGGAGAGCA TGTCGCCCCC AGTGAAGCGG CAAATTCTCT TGAACAGGCT 
CAGGCAGCTG CCGAGCGACT AGGCTACCCT GTGCTGGTGC GAGCAGCCTT TGCCCTGGGT 
GGTCTCGGTT CTGGCTTTGC TTCCACCAAA GAGGAACTCT CAGCTCTTGT GGCTCCAGCT 
TTTGCCCATA CCAGTCAGGT GCTGATAGAC AAGTCTCTGA AGGGCTGGAA GGAGATTGAA 
TATGAGGTGG TGAGAGACGC CTATGGCAAC TGCGTAACGG TATGCAACAT GGAGAACTTA 
GACCCACTGG GCATTCATAC TGGTGAATCC ATAGTGGTGG CTCCGAGCCA GACACTGAAT 
GACAGAGAGT ACCAGCTTCT GCGACGGACA GCTATCAAGG TCACTCAGCA CCTGGGCATT 
GTCGGGGAGT GCAATGTACA GTATGCTTTG AACCCCGAGT CTGAGCAGTA TTACATCATT 
GAAGTGAATG CCAGGCTCTC CCGAAGCTCT GCCCTGGCCA GTAAGGCCAC AGGCTATCCA 
CTAGCCTATG TGGCAGCCAA GCTAGCATTG GGCATTCCCC TGCCAGAGCT CAGAAACTCT 
GTTACGGGAG GAACAGCAGC CTTTGAGCCC AGCCTGGACT ACTGCGTGGT AAAGATTCCT 
CGCTGGGACC TCAGCAAGTT CCTACGTGTC AGTACGAAGA TTGGGAGTTG CATGAAGAGT 
GTTGGCGAGG TCATGGGCAT CGGGCGGTCC TTTGAGGAGG CCTTCCAAAA GGCCCTGCGC 
ATGGTCGATG AGAACTGCGT AGGCTTCGAC CATACAGTAA AGCCAGTCAG CGACATGGAG 
CTGGAGACTC CGACGGATAA GCGGATCTTT GTGGTGGCTG CCGCTCTGTG GGCTGGCTAC 
TCTGTGGAGC GCCTCTACGA GCTCACCCGC ATTGATTGCT GGTTCCTGCA CCGGATGAAG 
CGCATTGTGA CCCACGCCCA GTTGCTGGAA CAACACCGTG GACAAGCTTT ACCCCAAGAC 
CTGCTGCACC AGGCCAAGTG CCTCGGCTTC TCAGACAAGC AGATTGCCCT GGCAGTCCTG 
AGCACAGAGC TGGCTGTTCG GAAGCTGCGT CAGGAACTGG GGATCTGCCC AGCGGTGAAA 
CAGATTGACA CAGTTGCAGC TGAGTGGCCA GCCCAGACAA ATTACCTGTA CCTGACATAC 
TGGGGCAACA CCCATGACCT CGACTTTCGG GCACCTCACG TCCTGGTCCT TGGCTCTGGT 
GTCTACCGGA TTGGCTCCAG TGTTGAGTTT GATTGGTGTG CTGTGGGTTG CATCCAGCAG 
CTCCGGAAGA TGGGTTATAA GACCATCATG GTGAACTACA ACCCAGAGAC AGTCAGCACC 
GACTATGATA TGTGTGACCG ACTCTACTTT GATGAGATCT CTTTTGAGGT GGTGATGGAC 
ATCTATGAGT TAGAGAACCC CGAAGGTGTG ATCCTGTCCA TGGGTGGGCA GCTGCCCAAC 
AACATGGCCA TGGCTTTGCA TCGGCAGCAG TGCCGAGTGC TGGGCACCTC CCCCGAAGCC 
ATCGACTCAG CCGAGAACCG ATTCAAGTTC TCCCGGCTCC TAGATACCAT CGGTATCAGC 
CAGCCTCAGT GGCGAGAGCT CAGTGACCTT GAGTCTGCTC GCCAGTTCTG CCACACGGTC 
GGGTACCCCT GCGTAGTGCG CCCCTCCTAT GTGCTCAGCG GTGCTGCTAT GAATGTGGCG 
TACACCGACG GGGACCTGGA GCGTTTCCTG AGCAGTGCGG CCGCCGTCTC TAAGGAGCAC 
CCCGTAGTCA TCTCCAAGTT CATTCAGGAA GCAAAGGAGA TCGACGTGGA CGCGGTGGCC 
TGTGACGGCA TCGTGTCGGC CATTGCCATC TCTGAGCACG TGGAGAATGC AGGTGTGCAT 
TCAGGTGATG CCACGCTGGT GACCCCCCCA CAAGACATCA CCCCGAAAAC TCTGGAGCGG 
ATCAAAGCCA TTGTGCATGC TGTGGGCCAG GAGCTACAGG TTACAGGACC CTTCAATCTG 
CAGCTCATTG CCAAGGATGA CCAGCTGAAG GTTATTGAAT GCAACGTGCG TGTATCTCGC 
TCTTTCCCCT TCGTGTCCAA GACACTGGGT GTTGATCTAG TAGCCTTGGC CACAAGGATC 
ATCATGGGGG AGAAAGTAGA GCCCGTGGGG CTCATGACTG GCTCTGGAGT CGTGGGAGTA 
AAGGTACCTC AGTTCTCCTT CTCACGCTTG GCCGGTGCCG ACGTGGTACT TGGTGTGGAG 
ATGACCAGTA CTGGAGAGGT GGCCGGCTTC GGAGAGAGCC GTTGTGAAGC CTACCTCAAA 
GCCATGCTCA GCACTGGCTT TAAGATCCCA GAGAAGAACA TCCTGCTGAC TATTGGCAGC 
TACAAGAACA AAAGTGAGCT GCTCCCAACT GTGCGGTTGC TGGAGAGCCT GGGCTACAGC 
CTCTATGCCA GCCTGGGTAC GGCAGACTTC TACACTGAGC ACGGGGTCAA GGTAACAGCT 
GTGGACTGGC ACTTTGAAGA GGCGGTGGAT GGTGAGTGCC CACCACAACG GAGCATCTTG 
GATCAGCTGG CTGAAAATCA CTTTGAGCTA GTGATTAACC TGTCCATGCG TGGGGCTGGG 
GGTCGGCGTC TTTCCTCCTT CGTTACCAAG GGCTACCGTA CCCGGCGTCT GGCCGCTGAT 
TTCTCTGTGC CTCTCATCAT TGACATCAAG TGCACCAAAC TCTTTGTGGA GGCCCTAGGC 
CAGATCGGCC CAGCCCCTCC TTTGAAAGTG CACGTTGACT GCATGACCTC CCAGAAGCTA 
GTGCGCCTGC CCGGATTGAT TGATGTCCAC GTGCACCTTC GGGAACCAGG TGGGACACAC 
AAAGAGGACT TTGCCTCAGG CACAGCTGCT GCCTTGGCTG GGGGTGTCAC CATGGTTTGT 
GCTATGCCTA ATACCCGACC CCCCATCATT GATGCTCCTG CTCTCGCCCT TGCCCAGAAG 
CTGGCAGAGG CTGGCGCCCG CTGTGACTTT ACCTTATTCC TTGGAGCCTC ATCTGAGAAC 
GCGGGGACTC TGGGTGCTGT GGCCGGCTCT GCAGCAGGCC TGAAGCTCTA CCTCAACGAG 
ACCTTCTCTG AGCTGCGGCT GGACAGTGTG GCCCAGTGGA TGGAGCATTT TGAAACCTGG 
CCTGCCCACC TCCCCATTGT GGCCCATGCA GAGCGGCAGA GTGTCGCTGC GGTCCTCATG 
GTGGCTCAGC TGACCCAGCG TCCAGTGCAC ATATGTCATG TGGCTCGGAA GGAAGAGATC 
CTGTTGATTA AAACTGCAAA GGCGCAGGGG CTGCCAGTGA CCTGTGAGGT CGCTCCTCAC 
CACCTGTTCC TAAACCGGGA AGACTTGGAG CGCCTGGGAC CTGGGAAGGG AGAGGTCCGT 
CCTGAGCTTG GGTCCCGAGA GGATATGGAA GCCCTGTGGG AGAATATGGC AGTCATCGAC 
TGTTTTGCCT CAGACCACGC TCCCCATACC TTGGAAGAGA AGTGTGGGCC CAAGCCTCCA 
CCTGGCTTCC CGGGCCTAGA GACCATGTTG CCGCTGCTGC TGACAGCTGT GAGCGAGGGC 
CGCCTCAGTC TGGATGACCT GCTGCAGCGA TTGCACCACA ACCCTCGGCG GATCTTCCAC 
CTGCCTCTCC AGGAGGACAC CTATGTAGAG GTGGATCTGG AGCATGAGTG GACAGTCCCC 
AGCCACATGC CCTTCTCTAA GGCCCGCTGG ACCCCATTTG AAGGACAGAA GGTGAAGGGC 
ACAGTGCGCC GTGTGGTCTT GCGAGGGGAG GTCGCCTATA TTGATGGACA GGTGCTGGTA 
CCCCCGGGCT ATGGACAAGA TGTACGGAAG TGGCCGCAGG GGGTTGTTCC CCAGCCCCCT 
CCTTCAACTC CCGCCACCAC CGAAATCACC ACGACACCTG AGCGACCACG CCGAGTCATC 
CCGGGCCTTC CTGATGGCCG CTTCCACCTG CCACCCCGCA TCCACCGGGC CTCTGACCCA 
GGTCTGCCAG CTCTGTACCT CCGCCCAGGA GCTGGGATCT CATGGGGCAG CAGAGCATGG 
GCTGAGGAAC CTAAAGAGAA GCCACCCAGG AAGGTAGTGG AACCAGAGCT GATGGGAACC 
CCTGATGGTC CCTGCTACCC TGCACCACCA GTACCTAGAC AGGCATCACC CCAGAACCTA 
GGGTCTTCTG GCCTGCTGCA CCCCCAGATG TCGCCCCTAC TGCACTCCCT AGTGGGCCAG 
CACATCCTGT CTGTCAAGCA GTTCACTAAG GATCAGATGT CTCATCTGTT CAATGTGGCG 
CACACGCTAC GGATGATGGT ACAGAAGGAG CGGAGCCTCG ACATCCTGAA GGGGAAGGTC 
ATGGCCTCCA TGTTCTACGA GGTGAGCACC CGCACCAGTA GCTCCTTCGC AGCAGCCATG 
GCCCGGCTCG GGGGCGCTGT TCTCAGCTTC TCAGAAGCTA CATCCTCCGT CCAAAAGGGC 
GAGTCCCTTG CTGACTCGGT GCAGACCATG AGTTGCTATG CTGATGTCAT TGTGCTCCGA 
CACCCTCAGC CTGGAGCCGT GGAGCTGGCA GCCAAGCACT GTCGCAGGCC AGTGATCAAT 
GCTGGGGATG GAGTTGGGGA GCACCCCACA CAGGCCCTGC TGGACATCTT CACCATTCGA 
GAAGAGCTGG GGACTGTCAA TGGCATGACG ATCACTATGG TCGGTGACCT GAAGCATGGA 
CGCACAGTGC ACTCCCTGGC CTGCCTGCTC ACCCAGTACC GTGTGAGCCT ACGCTACGTG 
GCACCTCCCA GCCTGCGCAT GCCACCCAGT GTACGGGACT TTGTGGCCTC CCGAGGCACC 
AAGCAGGAGG AGTTTGAGAG CATCGAGGAG GCGCTGCCCG ACACAGACGT GCTCTACATG 
ACTCGGATCC AGAAAGAACG ATTTGGCTCT GTCCAGGAAT ATGAAGCTTG CTTTGGTCAG 
TTCATCCTCA CTCCTCACAT CATGACCCGG GCCAAGAAGA AGATGGTGGT GATGCACCCA 
ATGCCCCGTG TCAATGAGAT AAGTGTGGAG GTGGACTCGG ACCCCCGGGC AGCCTACTTC 
CGCCAAGCTG AGAACGGCAT GTACATCCGC ATGGCCCTGC TTGCCACCGT GCTTGGCCGT 
TTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cad ( NM_001289523.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001276452.1 Mus musculus carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad), transcript variant 3, mRNA.

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CloneID	OMu14731
Clone ID Related Accession (Same CDS sequence)	NM_001289523.1
Accession Version	NM_001289523.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6495bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-07
Organism	Mus musculus(house mouse)
Product	CAD protein isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC109608.14. Transcript Variant: This variant (3) uses an alternate in-frame splice site, compared to variant 1. The encoded isoform (3) is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC144973.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849382 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCCC TGGTGTTGGA GGACGGGTCG GTGCTGCAGG GCCGGCCCTT TGGGGCCGCT 
GTGTCGACTG CTGGGGAAGT GGTGTTTCAG ACCGGCATGG TCGGCTACCC AGAGGCCCTC 
ACTGACCCTT CCTACAAAGC TCAGATCCTA GTGCTAACGT ATCCTCTCAT CGGCAACTAC 
GGCATTCCCT CAGATGAAGA GGACGAGTTC GGCCTGAGCA AGTGGTTTGA ATCCTCGGAG 
ATCCACGTGG CGGGACTTGT AGTGGGAGAA TGCTGTCCCA CACCCAGCCA CTGGAGTGCC 
AACTGCACCC TGCATGAGTG GCTGCAACAG CGTGGCATCC CCGGCCTGCA AGGAGTGGAC 
ACTCGGGAGC TGACTAAGAA GTTGCGGGAG CAAGGGTCTC TTTTGGGGAA GCTGGTCCAG 
AAAGGGACAG AGCCGTCAGC CCTGCCATTC GTGGACCCCA ATGCCAGGCC CCTGGCACCA 
GAGGTCTCTA TTAAGACTCC ACGGGTATTC AATGCAGGGG GTGCCCCTCG GATCTGTGCT 
CTGGATTGTG GCCTCAAGTA TAATCAGATC CGATGTCTCT GCCAGCTTGG GGCTGAAGTT 
ACCGTGGTAC CCTGGGACCA CGAGTTAGAC AGTCAGAAAT ATGACGGTCT CTTCCTAAGT 
AATGGACCCG GTGACCCTGC CTCTTACCCG GGTGTGGTGT CCACACTGAG CCGAGTCTTG 
TCTGAACCTA ACCCCCGACC TGTGTTTGGA ATCTGCCTTG GACACCAGCT GTTGGCTTTA 
GCCATCGGGG CCAAAACTTA CAAGATGAGA TACGGAAACC GAGGCCACAA CCAGCCCTGT 
TTACTGGTGG GCACTGGGCG CTGCTTTCTG ACGTCCCAGA ATCATGGGTT TGCAGTGGAC 
GCAGATTCGC TGCCAGCAGG CTGGGCTCCT CTGTTCACCA ATGCCAATGA CTGTTCCAAT 
GAAGGCATAG TGCACGACAG CCTGCCCTTC TTCAGTGTCC AGTTTCACCC AGAGCACCGG 
GCCGGCCCTT CAGATATGGA ACTGCTCTTC GATGTATTTC TAGAAACTGT GAGAGAGGCT 
GCAGCTGGGA ACATTGGGGG CCAGACAGTA AGAGAACGCT TGGCTCAGCG CCTCTGTCCC 
CCTGAGCTCC CCATTCCTGG TTCTGGGCTC CCGCCTCCAC GAAAGGTTCT AATCCTAGGC 
TCAGGGGGCC TCTCCATTGG CCAAGCTGGA GAATTTGACT ACTCGGGCTC TCAGGCAATT 
AAAGCCCTAA AGGAGGAAAA CATCCAGACA TTACTAATCA ACCCCAACAT TGCCACAGTG 
CAGACTTCTC AGGGGCTGGC AGACAAGGTC TATTTCCTTC CCATAACACT TCACTACGTA 
ACCCAGGTAA TTCGTAATGA ACGCCCAGAT GGTGTGTTAC TGACTTTTGG GGGTCAAACA 
GCCCTGAACT GCGGTGTGGA GCTGACCAAA GCTGGGGTAC TGGCTCGATA TGGGGTCCGG 
GTCTTGGGTA CACCCGTGGA GACCATTGAA CTGACTGAGG ACCGACGAGC CTTCGCTGCC 
AGGATGGCTG AGATCGGAGA GCATGTCGCC CCCAGTGAAG CGGCAAATTC TCTTGAACAG 
GCTCAGGCAG CTGCCGAGCG ACTAGGCTAC CCTGTGCTGG TGCGAGCAGC CTTTGCCCTG 
GGTGGTCTCG GTTCTGGCTT TGCTTCCACC AAAGAGGAAC TCTCAGCTCT TGTGGCTCCA 
GCTTTTGCCC ATACCAGTCA GGTGCTGATA GACAAGTCTC TGAAGGGCTG GAAGGAGATT 
GAATATGAGG TGGTGAGAGA CGCCTATGGC AACTGCGTAA CGGTATGCAA CATGGAGAAC 
TTAGACCCAC TGGGCATTCA TACTGGTGAA TCCATAGTGG TGGCTCCGAG CCAGACACTG 
AATGACAGAG AGTACCAGCT TCTGCGACGG ACAGCTATCA AGGTCACTCA GCACCTGGGC 
ATTGTCGGGG AGTGCAATGT ACAGTATGCT TTGAACCCCG AGTCTGAGCA GTATTACATC 
ATTGAAGTGA ATGCCAGGCT CTCCCGAAGC TCTGCCCTGG CCAGTAAGGC CACAGGCTAT 
CCACTAGCCT ATGTGGCAGC CAAGCTAGCA TTGGGCATTC CCCTGCCAGA GCTCAGAAAC 
TCTGTTACGG GAGGAACAGC AGCCTTTGAG CCCAGCCTGG ACTACTGCGT GGTAAAGATT 
CCTCGCTGGG ACCTCAGCAA GTTCCTACGT GTCAGTACGA AGATTGGGAG TTGCATGAAG 
AGTGTTGGCG AGGTCATGGG CATCGGGCGG TCCTTTGAGG AGGCCTTCCA AAAGGCCCTG 
CGCATGGTCG ATGAGAACTG CGTAGGCTTC GACCATACAG TAAAGCCAGT CAGCGACATG 
GAGCTGGAGA CTCCGACGGA TAAGCGGATC TTTGTGGTGG CTGCCGCTCT GTGGGCTGGC 
TACTCTGTGG AGCGCCTCTA CGAGCTCACC CGCATTGATT GCTGGTTCCT GCACCGGATG 
AAGCGCATTG TGACCCACGC CCAGTTGCTG GAACAACACC GTGGACAAGC TTTACCCCAA 
GACCTGCTGC ACCAGGCCAA GTGCCTCGGC TTCTCAGACA AGCAGATTGC CCTGGCAGTC 
CTGAGCACAG AGCTGGCTGT TCGGAAGCTG CGTCAGGAAC TGGGGATCTG CCCAGCGGTG 
AAACAGATTG ACACAGTTGC AGCTGAGTGG CCAGCCCAGA CAAATTACCT GTACCTGACA 
TACTGGGGCA ACACCCATGA CCTCGACTTT CGGGCACCTC ACGTCCTGGT CCTTGGCTCT 
GGTGTCTACC GGATTGGCTC CAGTGTTGAG TTTGATTGGT GTGCTGTGGG TTGCATCCAG 
CAGCTCCGGA AGATGGGTTA TAAGACCATC ATGGTGAACT ACAACCCAGA GACAGTCAGC 
ACCGACTATG ATATGTGTGA CCGACTCTAC TTTGATGAGA TCTCTTTTGA GGTGGTGATG 
GACATCTATG AGTTAGAGAA CCCCGAAGGT GTGATCCTGT CCATGGGTGG GCAGCTGCCC 
AACAACATGG CCATGGCTTT GCATCGGCAG CAGTGCCGAG TGCTGGGCAC CTCCCCCGAA 
GCCATCGACT CAGCCGAGAA CCGATTCAAG TTCTCCCGGC TCCTAGATAC CATCGGTATC 
AGCCAGCCTC AGTGGCGAGA GCTCAGTGAC CTTGAGTCTG CTCGCCAGTT CTGCCACACG 
GTCGGGTACC CCTGCGTAGT GCGCCCCTCC TATGTGCTCA GCGGTGCTGC TATGAATGTG 
GCGTACACCG ACGGGGACCT GGAGCGTTTC CTGAGCAGTG CGGCCGCCGT CTCTAAGGAG 
CACCCCGTAG TCATCTCCAA GTTCATTCAG GAAGCAAAGG AGATCGACGT GGACGCGGTG 
GCCTGTGACG GCATCGTGTC GGCCATTGCC ATCTCTGAGC ACGTGGAGAA TGCAGGTGTG 
CATTCAGGTG ATGCCACGCT GGTGACCCCC CCACAAGACA TCACCCCGAA AACTCTGGAG 
CGGATCAAAG CCATTGTGCA TGCTGTGGGC CAGGAGCTAC AGGTTACAGG ACCCTTCAAT 
CTGCAGCTCA TTGCCAAGGA TGACCAGCTG AAGGTTATTG AATGCAACGT GCGTGTATCT 
CGCTCTTTCC CCTTCGTGTC CAAGACACTG GGTGTTGATC TAGTAGCCTT GGCCACAAGG 
ATCATCATGG GGGAGAAAGT AGAGCCCGTG GGGCTCATGA CTGGCTCTGG AGTCGTGGGA 
GTAAAGGTAC CTCAGTTCTC CTTCTCACGC TTGGCCGGTG CCGACGTGGT ACTTGGTGTG 
GAGATGACCA GTACTGGAGA GGTGGCCGGC TTCGGAGAGA GCCGTTGTGA AGCCTACCTC 
AAAGCCATGC TCAGCACTGG CTTTAAGATC CCAGAGAAGA ACATCCTGCT GACTATTGGC 
AGCTACAAGA ACAAAAGTGA GCTGCTCCCA ACTGTGCGGT TGCTGGAGAG CCTGGGCTAC 
AGCCTCTATG CCAGCCTGGG TACGGCAGAC TTCTACACTG AGCACGGGGT CAAGGTAACA 
GCTGTGGACT GGCACTTTGA AGAGGCGGTG GATGGTGAGT GCCCACCACA ACGGAGCATC 
TTGGATCAGC TGGCTGAAAA TCACTTTGAG CTAGTGATTA ACCTGTCCAT GCGTGGGGCT 
GGGGGTCGGC GTCTTTCCTC CTTCGTTACC AAGGGCTACC GTACCCGGCG TCTGGCCGCT 
GATTTCTCTG TGCCTCTCAT CATTGACATC AAGTGCACCA AACTCTTTGT GGAGGCCCTA 
GGCCAGATCG GCCCAGCCCC TCCTTTGAAA GTGCACGTTG ACTGCATGAC CTCCCAGAAG 
CTAGTGCGCC TGCCCGGATT GATTGATGTC CACGTGCACC TTCGGGAACC AGGTGGGACA 
CACAAAGAGG ACTTTGCCTC AGGCACAGCT GCTGCCTTGG CTGGGGGTGT CACCATGGTT 
TGTGCTATGC CTAATACCCG ACCCCCCATC ATTGATGCTC CTGCTCTCGC CCTTGCCCAG 
AAGCTGGCAG AGGCTGGCGC CCGCTGTGAC TTTACCTTAT TCCTTGGAGC CTCATCTGAG 
AACGCGGGGA CTCTGGGTGC TGTGGCCGGC TCTGCAGCAG GCCTGAAGCT CTACCTCAAC 
GAGACCTTCT CTGAGCTGCG GCTGGACAGT GTGGCCCAGT GGATGGAGCA TTTTGAAACC 
TGGCCTGCCC ACCTCCCCAT TGTGGCCCAT GCAGAGCGGC AGAGTGTCGC TGCGGTCCTC 
ATGGTGGCTC AGCTGACCCA GCGTCCAGTG CACATATGTC ATGTGGCTCG GAAGGAAGAG 
ATCCTGTTGA TTAAAACTGC AAAGGCGCAG GGGCTGCCAG TGACCTGTGA GGTCGCTCCT 
CACCACCTGT TCCTAAACCG GGAAGACTTG GAGCGCCTGG GACCTGGGAA GGGAGAGGTC 
CGTCCTGAGC TTGGGTCCCG AGAGGATATG GAAGCCCTGT GGGAGAATAT GGCAGTCATC 
GACTGTTTTG CCTCAGACCA CGCTCCCCAT ACCTTGGAAG AGAAGTGTGG GCCCAAGCCT 
CCACCTGGCT TCCCGGGCCT AGAGACCATG TTGCCGCTGC TGCTGACAGC TGTGAGCGAG 
GGCCGCCTCA GTCTGGATGA CCTGCTGCAG CGATTGCACC ACAACCCTCG GCGGATCTTC 
CACCTGCCTC TCCAGGAGGA CACCTATGTA GAGGTGGATC TGGAGCATGA GTGGACAGTC 
CCCAGCCACA TGCCCTTCTC TAAGGCCCGC TGGACCCCAT TTGAAGGACA GAAGGTGAAG 
GGCACAGTGC GCCGTGTGGT CTTGCGAGGG GAGGTCGCCT ATATTGATGG ACAGGTGCTG 
GTACCCCCGG GCTATGGACA AGATGTACGG AAGTGGCCGC AGGGGGTTGT TCCCCAGCCC 
CCTCCTTCAA CTCCCGCCAC CACCGAAATC ACCACGACAC CTGAGCGACC ACGCCGAGTC 
ATCCCGGGCC TTCCTGATGG CCGCTTCCAC CTGCCACCCC GCATCCACCG GGCCTCTGAC 
CCAGGTCTGC CAGCTGAGGA ACCTAAAGAG AAGCCACCCA GGAAGGTAGT GGAACCAGAG 
CTGATGGGAA CCCCTGATGG TCCCTGCTAC CCTGCACCAC CAGTACCTAG ACAGGCATCA 
CCCCAGAACC TAGGGTCTTC TGGCCTGCTG CACCCCCAGA TGTCGCCCCT ACTGCACTCC 
CTAGTGGGCC AGCACATCCT GTCTGTCAAG CAGTTCACTA AGGATCAGAT GTCTCATCTG 
TTCAATGTGG CGCACACGCT ACGGATGATG GTACAGAAGG AGCGGAGCCT CGACATCCTG 
AAGGGGAAGG TCATGGCCTC CATGTTCTAC GAGCTGGCAG CCAAGCACTG TCGCAGGCCA 
GTGATCAATG CTGGGGATGG AGTTGGGGAG CACCCCACAC AGGCCCTGCT GGACATCTTC 
ACCATTCGAG AAGAGCTGGG GACTGTCAAT GGCATGACGA TCACTATGGT CGGTGACCTG 
AAGCATGGAC GCACAGTGCA CTCCCTGGCC TGCCTGCTCA CCCAGTACCG TGTGAGCCTA 
CGCTACGTGG CACCTCCCAG CCTGCGCATG CCACCCAGTG TACGGGACTT TGTGGCCTCC 
CGAGGCACCA AGCAGGAGGA GTTTGAGAGC ATCGAGGAGG CGCTGCCCGA CACAGACGTG 
CTCTACATGA CTCGGATCCA GAAAGAACGA TTTGGCTCTG TCCAGGAATA TGAAGCTTGC 
TTTGGTCAGT TCATCCTCAC TCCTCACATC ATGACCCGGG CCAAGAAGAA GATGGTGGTG 
ATGCACCCAA TGCCCCGTGT CAATGAGATA AGTGTGGAGG TGGACTCGGA CCCCCGGGCA 
GCCTACTTCC GCCAAGCTGA GAACGGCATG TACATCCGCA TGGCCCTGCT TGCCACCGTG 
CTTGGCCGTT TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001276452.1
CDS	176..6670
Translation	MAALVLEDGSVLQGRPFGAAVSTAGEVVFQTGMVGYPEALTDPSYKAQILVLTYPLIGNYGIPSDEEDEFGLSKWFESSEIHVAGLVVGECCPTPSHWSANCTLHEWLQQRGIPGLQGVDTRELTKKLREQGSLLGKLVQKGTEPSALPFVDPNARPLAPEVSIKTPRVFNAGGAPRICALDCGLKYNQIRCLCQLGAEVTVVPWDHELDSQKYDGLFLSNGPGDPASYPGVVSTLSRVLSEPNPRPVFGICLGHQLLALAIGAKTYKMRYGNRGHNQPCLLVGTGRCFLTSQNHGFAVDADSLPAGWAPLFTNANDCSNEGIVHDSLPFFSVQFHPEHRAGPSDMELLFDVFLETVREAAAGNIGGQTVRERLAQRLCPPELPIPGSGLPPPRKVLILGSGGLSIGQAGEFDYSGSQAIKALKEENIQTLLINPNIATVQTSQGLADKVYFLPITLHYVTQVIRNERPDGVLLTFGGQTALNCGVELTKAGVLARYGVRVLGTPVETIELTEDRRAFAARMAEIGEHVAPSEAANSLEQAQAAAERLGYPVLVRAAFALGGLGSGFASTKEELSALVAPAFAHTSQVLIDKSLKGWKEIEYEVVRDAYGNCVTVCNMENLDPLGIHTGESIVVAPSQTLNDREYQLLRRTAIKVTQHLGIVGECNVQYALNPESEQYYIIEVNARLSRSSALASKATGYPLAYVAAKLALGIPLPELRNSVTGGTAAFEPSLDYCVVKIPRWDLSKFLRVSTKIGSCMKSVGEVMGIGRSFEEAFQKALRMVDENCVGFDHTVKPVSDMELETPTDKRIFVVAAALWAGYSVERLYELTRIDCWFLHRMKRIVTHAQLLEQHRGQALPQDLLHQAKCLGFSDKQIALAVLSTELAVRKLRQELGICPAVKQIDTVAAEWPAQTNYLYLTYWGNTHDLDFRAPHVLVLGSGVYRIGSSVEFDWCAVGCIQQLRKMGYKTIMVNYNPETVSTDYDMCDRLYFDEISFEVVMDIYELENPEGVILSMGGQLPNNMAMALHRQQCRVLGTSPEAIDSAENRFKFSRLLDTIGISQPQWRELSDLESARQFCHTVGYPCVVRPSYVLSGAAMNVAYTDGDLERFLSSAAAVSKEHPVVISKFIQEAKEIDVDAVACDGIVSAIAISEHVENAGVHSGDATLVTPPQDITPKTLERIKAIVHAVGQELQVTGPFNLQLIAKDDQLKVIECNVRVSRSFPFVSKTLGVDLVALATRIIMGEKVEPVGLMTGSGVVGVKVPQFSFSRLAGADVVLGVEMTSTGEVAGFGESRCEAYLKAMLSTGFKIPEKNILLTIGSYKNKSELLPTVRLLESLGYSLYASLGTADFYTEHGVKVTAVDWHFEEAVDGECPPQRSILDQLAENHFELVINLSMRGAGGRRLSSFVTKGYRTRRLAADFSVPLIIDIKCTKLFVEALGQIGPAPPLKVHVDCMTSQKLVRLPGLIDVHVHLREPGGTHKEDFASGTAAALAGGVTMVCAMPNTRPPIIDAPALALAQKLAEAGARCDFTLFLGASSENAGTLGAVAGSAAGLKLYLNETFSELRLDSVAQWMEHFETWPAHLPIVAHAERQSVAAVLMVAQLTQRPVHICHVARKEEILLIKTAKAQGLPVTCEVAPHHLFLNREDLERLGPGKGEVRPELGSREDMEALWENMAVIDCFASDHAPHTLEEKCGPKPPPGFPGLETMLPLLLTAVSEGRLSLDDLLQRLHHNPRRIFHLPLQEDTYVEVDLEHEWTVPSHMPFSKARWTPFEGQKVKGTVRRVVLRGEVAYIDGQVLVPPGYGQDVRKWPQGVVPQPPPSTPATTEITTTPERPRRVIPGLPDGRFHLPPRIHRASDPGLPAEEPKEKPPRKVVEPELMGTPDGPCYPAPPVPRQASPQNLGSSGLLHPQMSPLLHSLVGQHILSVKQFTKDQMSHLFNVAHTLRMMVQKERSLDILKGKVMASMFYELAAKHCRRPVINAGDGVGEHPTQALLDIFTIREELGTVNGMTITMVGDLKHGRTVHSLACLLTQYRVSLRYVAPPSLRMPPSVRDFVASRGTKQEEFESIEEALPDTDVLYMTRIQKERFGSVQEYEACFGQFILTPHIMTRAKKKMVVMHPMPRVNEISVEVDSDPRAAYFRQAENGMYIRMALLATVLGRF

Target ORF information:

RefSeq Version	NM_001289523.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001289523.1

ORF Insert Sequence:

ATGGCGGCCC TGGTGTTGGA GGACGGGTCG GTGCTGCAGG GCCGGCCCTT TGGGGCCGCT 
GTGTCGACTG CTGGGGAAGT GGTGTTTCAG ACCGGCATGG TCGGCTACCC AGAGGCCCTC 
ACTGACCCTT CCTACAAAGC TCAGATCCTA GTGCTAACGT ATCCTCTCAT CGGCAACTAC 
GGCATTCCCT CAGATGAAGA GGACGAGTTC GGCCTGAGCA AGTGGTTTGA ATCCTCGGAG 
ATCCACGTGG CGGGACTTGT AGTGGGAGAA TGCTGTCCCA CACCCAGCCA CTGGAGTGCC 
AACTGCACCC TGCATGAGTG GCTGCAACAG CGTGGCATCC CCGGCCTGCA AGGAGTGGAC 
ACTCGGGAGC TGACTAAGAA GTTGCGGGAG CAAGGGTCTC TTTTGGGGAA GCTGGTCCAG 
AAAGGGACAG AGCCGTCAGC CCTGCCATTC GTGGACCCCA ATGCCAGGCC CCTGGCACCA 
GAGGTCTCTA TTAAGACTCC ACGGGTATTC AATGCAGGGG GTGCCCCTCG GATCTGTGCT 
CTGGATTGTG GCCTCAAGTA TAATCAGATC CGATGTCTCT GCCAGCTTGG GGCTGAAGTT 
ACCGTGGTAC CCTGGGACCA CGAGTTAGAC AGTCAGAAAT ATGACGGTCT CTTCCTAAGT 
AATGGACCCG GTGACCCTGC CTCTTACCCG GGTGTGGTGT CCACACTGAG CCGAGTCTTG 
TCTGAACCTA ACCCCCGACC TGTGTTTGGA ATCTGCCTTG GACACCAGCT GTTGGCTTTA 
GCCATCGGGG CCAAAACTTA CAAGATGAGA TACGGAAACC GAGGCCACAA CCAGCCCTGT 
TTACTGGTGG GCACTGGGCG CTGCTTTCTG ACGTCCCAGA ATCATGGGTT TGCAGTGGAC 
GCAGATTCGC TGCCAGCAGG CTGGGCTCCT CTGTTCACCA ATGCCAATGA CTGTTCCAAT 
GAAGGCATAG TGCACGACAG CCTGCCCTTC TTCAGTGTCC AGTTTCACCC AGAGCACCGG 
GCCGGCCCTT CAGATATGGA ACTGCTCTTC GATGTATTTC TAGAAACTGT GAGAGAGGCT 
GCAGCTGGGA ACATTGGGGG CCAGACAGTA AGAGAACGCT TGGCTCAGCG CCTCTGTCCC 
CCTGAGCTCC CCATTCCTGG TTCTGGGCTC CCGCCTCCAC GAAAGGTTCT AATCCTAGGC 
TCAGGGGGCC TCTCCATTGG CCAAGCTGGA GAATTTGACT ACTCGGGCTC TCAGGCAATT 
AAAGCCCTAA AGGAGGAAAA CATCCAGACA TTACTAATCA ACCCCAACAT TGCCACAGTG 
CAGACTTCTC AGGGGCTGGC AGACAAGGTC TATTTCCTTC CCATAACACT TCACTACGTA 
ACCCAGGTAA TTCGTAATGA ACGCCCAGAT GGTGTGTTAC TGACTTTTGG GGGTCAAACA 
GCCCTGAACT GCGGTGTGGA GCTGACCAAA GCTGGGGTAC TGGCTCGATA TGGGGTCCGG 
GTCTTGGGTA CACCCGTGGA GACCATTGAA CTGACTGAGG ACCGACGAGC CTTCGCTGCC 
AGGATGGCTG AGATCGGAGA GCATGTCGCC CCCAGTGAAG CGGCAAATTC TCTTGAACAG 
GCTCAGGCAG CTGCCGAGCG ACTAGGCTAC CCTGTGCTGG TGCGAGCAGC CTTTGCCCTG 
GGTGGTCTCG GTTCTGGCTT TGCTTCCACC AAAGAGGAAC TCTCAGCTCT TGTGGCTCCA 
GCTTTTGCCC ATACCAGTCA GGTGCTGATA GACAAGTCTC TGAAGGGCTG GAAGGAGATT 
GAATATGAGG TGGTGAGAGA CGCCTATGGC AACTGCGTAA CGGTATGCAA CATGGAGAAC 
TTAGACCCAC TGGGCATTCA TACTGGTGAA TCCATAGTGG TGGCTCCGAG CCAGACACTG 
AATGACAGAG AGTACCAGCT TCTGCGACGG ACAGCTATCA AGGTCACTCA GCACCTGGGC 
ATTGTCGGGG AGTGCAATGT ACAGTATGCT TTGAACCCCG AGTCTGAGCA GTATTACATC 
ATTGAAGTGA ATGCCAGGCT CTCCCGAAGC TCTGCCCTGG CCAGTAAGGC CACAGGCTAT 
CCACTAGCCT ATGTGGCAGC CAAGCTAGCA TTGGGCATTC CCCTGCCAGA GCTCAGAAAC 
TCTGTTACGG GAGGAACAGC AGCCTTTGAG CCCAGCCTGG ACTACTGCGT GGTAAAGATT 
CCTCGCTGGG ACCTCAGCAA GTTCCTACGT GTCAGTACGA AGATTGGGAG TTGCATGAAG 
AGTGTTGGCG AGGTCATGGG CATCGGGCGG TCCTTTGAGG AGGCCTTCCA AAAGGCCCTG 
CGCATGGTCG ATGAGAACTG CGTAGGCTTC GACCATACAG TAAAGCCAGT CAGCGACATG 
GAGCTGGAGA CTCCGACGGA TAAGCGGATC TTTGTGGTGG CTGCCGCTCT GTGGGCTGGC 
TACTCTGTGG AGCGCCTCTA CGAGCTCACC CGCATTGATT GCTGGTTCCT GCACCGGATG 
AAGCGCATTG TGACCCACGC CCAGTTGCTG GAACAACACC GTGGACAAGC TTTACCCCAA 
GACCTGCTGC ACCAGGCCAA GTGCCTCGGC TTCTCAGACA AGCAGATTGC CCTGGCAGTC 
CTGAGCACAG AGCTGGCTGT TCGGAAGCTG CGTCAGGAAC TGGGGATCTG CCCAGCGGTG 
AAACAGATTG ACACAGTTGC AGCTGAGTGG CCAGCCCAGA CAAATTACCT GTACCTGACA 
TACTGGGGCA ACACCCATGA CCTCGACTTT CGGGCACCTC ACGTCCTGGT CCTTGGCTCT 
GGTGTCTACC GGATTGGCTC CAGTGTTGAG TTTGATTGGT GTGCTGTGGG TTGCATCCAG 
CAGCTCCGGA AGATGGGTTA TAAGACCATC ATGGTGAACT ACAACCCAGA GACAGTCAGC 
ACCGACTATG ATATGTGTGA CCGACTCTAC TTTGATGAGA TCTCTTTTGA GGTGGTGATG 
GACATCTATG AGTTAGAGAA CCCCGAAGGT GTGATCCTGT CCATGGGTGG GCAGCTGCCC 
AACAACATGG CCATGGCTTT GCATCGGCAG CAGTGCCGAG TGCTGGGCAC CTCCCCCGAA 
GCCATCGACT CAGCCGAGAA CCGATTCAAG TTCTCCCGGC TCCTAGATAC CATCGGTATC 
AGCCAGCCTC AGTGGCGAGA GCTCAGTGAC CTTGAGTCTG CTCGCCAGTT CTGCCACACG 
GTCGGGTACC CCTGCGTAGT GCGCCCCTCC TATGTGCTCA GCGGTGCTGC TATGAATGTG 
GCGTACACCG ACGGGGACCT GGAGCGTTTC CTGAGCAGTG CGGCCGCCGT CTCTAAGGAG 
CACCCCGTAG TCATCTCCAA GTTCATTCAG GAAGCAAAGG AGATCGACGT GGACGCGGTG 
GCCTGTGACG GCATCGTGTC GGCCATTGCC ATCTCTGAGC ACGTGGAGAA TGCAGGTGTG 
CATTCAGGTG ATGCCACGCT GGTGACCCCC CCACAAGACA TCACCCCGAA AACTCTGGAG 
CGGATCAAAG CCATTGTGCA TGCTGTGGGC CAGGAGCTAC AGGTTACAGG ACCCTTCAAT 
CTGCAGCTCA TTGCCAAGGA TGACCAGCTG AAGGTTATTG AATGCAACGT GCGTGTATCT 
CGCTCTTTCC CCTTCGTGTC CAAGACACTG GGTGTTGATC TAGTAGCCTT GGCCACAAGG 
ATCATCATGG GGGAGAAAGT AGAGCCCGTG GGGCTCATGA CTGGCTCTGG AGTCGTGGGA 
GTAAAGGTAC CTCAGTTCTC CTTCTCACGC TTGGCCGGTG CCGACGTGGT ACTTGGTGTG 
GAGATGACCA GTACTGGAGA GGTGGCCGGC TTCGGAGAGA GCCGTTGTGA AGCCTACCTC 
AAAGCCATGC TCAGCACTGG CTTTAAGATC CCAGAGAAGA ACATCCTGCT GACTATTGGC 
AGCTACAAGA ACAAAAGTGA GCTGCTCCCA ACTGTGCGGT TGCTGGAGAG CCTGGGCTAC 
AGCCTCTATG CCAGCCTGGG TACGGCAGAC TTCTACACTG AGCACGGGGT CAAGGTAACA 
GCTGTGGACT GGCACTTTGA AGAGGCGGTG GATGGTGAGT GCCCACCACA ACGGAGCATC 
TTGGATCAGC TGGCTGAAAA TCACTTTGAG CTAGTGATTA ACCTGTCCAT GCGTGGGGCT 
GGGGGTCGGC GTCTTTCCTC CTTCGTTACC AAGGGCTACC GTACCCGGCG TCTGGCCGCT 
GATTTCTCTG TGCCTCTCAT CATTGACATC AAGTGCACCA AACTCTTTGT GGAGGCCCTA 
GGCCAGATCG GCCCAGCCCC TCCTTTGAAA GTGCACGTTG ACTGCATGAC CTCCCAGAAG 
CTAGTGCGCC TGCCCGGATT GATTGATGTC CACGTGCACC TTCGGGAACC AGGTGGGACA 
CACAAAGAGG ACTTTGCCTC AGGCACAGCT GCTGCCTTGG CTGGGGGTGT CACCATGGTT 
TGTGCTATGC CTAATACCCG ACCCCCCATC ATTGATGCTC CTGCTCTCGC CCTTGCCCAG 
AAGCTGGCAG AGGCTGGCGC CCGCTGTGAC TTTACCTTAT TCCTTGGAGC CTCATCTGAG 
AACGCGGGGA CTCTGGGTGC TGTGGCCGGC TCTGCAGCAG GCCTGAAGCT CTACCTCAAC 
GAGACCTTCT CTGAGCTGCG GCTGGACAGT GTGGCCCAGT GGATGGAGCA TTTTGAAACC 
TGGCCTGCCC ACCTCCCCAT TGTGGCCCAT GCAGAGCGGC AGAGTGTCGC TGCGGTCCTC 
ATGGTGGCTC AGCTGACCCA GCGTCCAGTG CACATATGTC ATGTGGCTCG GAAGGAAGAG 
ATCCTGTTGA TTAAAACTGC AAAGGCGCAG GGGCTGCCAG TGACCTGTGA GGTCGCTCCT 
CACCACCTGT TCCTAAACCG GGAAGACTTG GAGCGCCTGG GACCTGGGAA GGGAGAGGTC 
CGTCCTGAGC TTGGGTCCCG AGAGGATATG GAAGCCCTGT GGGAGAATAT GGCAGTCATC 
GACTGTTTTG CCTCAGACCA CGCTCCCCAT ACCTTGGAAG AGAAGTGTGG GCCCAAGCCT 
CCACCTGGCT TCCCGGGCCT AGAGACCATG TTGCCGCTGC TGCTGACAGC TGTGAGCGAG 
GGCCGCCTCA GTCTGGATGA CCTGCTGCAG CGATTGCACC ACAACCCTCG GCGGATCTTC 
CACCTGCCTC TCCAGGAGGA CACCTATGTA GAGGTGGATC TGGAGCATGA GTGGACAGTC 
CCCAGCCACA TGCCCTTCTC TAAGGCCCGC TGGACCCCAT TTGAAGGACA GAAGGTGAAG 
GGCACAGTGC GCCGTGTGGT CTTGCGAGGG GAGGTCGCCT ATATTGATGG ACAGGTGCTG 
GTACCCCCGG GCTATGGACA AGATGTACGG AAGTGGCCGC AGGGGGTTGT TCCCCAGCCC 
CCTCCTTCAA CTCCCGCCAC CACCGAAATC ACCACGACAC CTGAGCGACC ACGCCGAGTC 
ATCCCGGGCC TTCCTGATGG CCGCTTCCAC CTGCCACCCC GCATCCACCG GGCCTCTGAC 
CCAGGTCTGC CAGCTGAGGA ACCTAAAGAG AAGCCACCCA GGAAGGTAGT GGAACCAGAG 
CTGATGGGAA CCCCTGATGG TCCCTGCTAC CCTGCACCAC CAGTACCTAG ACAGGCATCA 
CCCCAGAACC TAGGGTCTTC TGGCCTGCTG CACCCCCAGA TGTCGCCCCT ACTGCACTCC 
CTAGTGGGCC AGCACATCCT GTCTGTCAAG CAGTTCACTA AGGATCAGAT GTCTCATCTG 
TTCAATGTGG CGCACACGCT ACGGATGATG GTACAGAAGG AGCGGAGCCT CGACATCCTG 
AAGGGGAAGG TCATGGCCTC CATGTTCTAC GAGCTGGCAG CCAAGCACTG TCGCAGGCCA 
GTGATCAATG CTGGGGATGG AGTTGGGGAG CACCCCACAC AGGCCCTGCT GGACATCTTC 
ACCATTCGAG AAGAGCTGGG GACTGTCAAT GGCATGACGA TCACTATGGT CGGTGACCTG 
AAGCATGGAC GCACAGTGCA CTCCCTGGCC TGCCTGCTCA CCCAGTACCG TGTGAGCCTA 
CGCTACGTGG CACCTCCCAG CCTGCGCATG CCACCCAGTG TACGGGACTT TGTGGCCTCC 
CGAGGCACCA AGCAGGAGGA GTTTGAGAGC ATCGAGGAGG CGCTGCCCGA CACAGACGTG 
CTCTACATGA CTCGGATCCA GAAAGAACGA TTTGGCTCTG TCCAGGAATA TGAAGCTTGC 
TTTGGTCAGT TCATCCTCAC TCCTCACATC ATGACCCGGG CCAAGAAGAA GATGGTGGTG 
ATGCACCCAA TGCCCCGTGT CAATGAGATA AGTGTGGAGG TGGACTCGGA CCCCCGGGCA 
GCCTACTTCC GCCAAGCTGA GAACGGCATG TACATCCGCA TGGCCCTGCT TGCCACCGTG 
CTTGGCCGTT TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cad ( NM_001289522.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001276451.1 Mus musculus carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad), transcript variant 2, mRNA.

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CloneID	OMu14732
Clone ID Related Accession (Same CDS sequence)	NM_001289522.1
Accession Version	NM_001289522.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-07
Organism	Mus musculus(house mouse)
Product	CAD protein isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC109608.14. Transcript Variant: This variant (2) lacks an alternate in-frame exon, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC144972.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCCC TGGTGTTGGA GGACGGGTCG GTGCTGCAGG GCCGGCCCTT TGGGGCCGCT 
GTGTCGACTG CTGGGGAAGT GGTGTTTCAG ACCGGCATGG TCGGCTACCC AGAGGCCCTC 
ACTGACCCTT CCTACAAAGC TCAGATCCTA GTGCTAACGT ATCCTCTCAT CGGCAACTAC 
GGCATTCCCT CAGATGAAGA GGACGAGTTC GGCCTGAGCA AGTGGTTTGA ATCCTCGGAG 
ATCCACGTGG CGGGACTTGT AGTGGGAGAA TGCTGTCCCA CACCCAGCCA CTGGAGTGCC 
AACTGCACCC TGCATGAGTG GCTGCAACAG CGTGGCATCC CCGGCCTGCA AGGAGTGGAC 
ACTCGGGAGC TGACTAAGAA GTTGCGGGAG CAAGGGTCTC TTTTGGGGAA GCTGGTCCAG 
AAAGGGACAG AGCCGTCAGC CCTGCCATTC GTGGACCCCA ATGCCAGGCC CCTGGCACCA 
GAGGTCTCTA TTAAGACTCC ACGGGTATTC AATGCAGGGG GTGCCCCTCG GATCTGTGCT 
CTGGATTGTG GCCTCAAGTA TAATCAGATC CGATGTCTCT GCCAGCTTGG GGCTGAAGTT 
ACCGTGGTAC CCTGGGACCA CGAGTTAGAC AGTCAGAAAT ATGACGGTCT CTTCCTAAGT 
AATGGACCCG GTGACCCTGC CTCTTACCCG GGTGTGGTGT CCACACTGAG CCGAGTCTTG 
TCTGAACCTA ACCCCCGACC TGTGTTTGGA ATCTGCCTTG GACACCAGCT GTTGGCTTTA 
GCCATCGGGG CCAAAACTTA CAAGATGAGA TACGGAAACC GAGGCCACAA CCAGCCCTGT 
TTACTGGTGG GCACTGGGCG CTGCTTTCTG ACGTCCCAGA ATCATGGGTT TGCAGTGGAC 
GCAGATTCGC TGCCAGCAGG CTGGGCTCCT CTGTTCACCA ATGCCAATGA CTGTTCCAAT 
GAAGGCATAG TGCACGACAG CCTGCCCTTC TTCAGTGTCC AGTTTCACCC AGAGCACCGG 
GCCGGCCCTT CAGATATGGA ACTGCTCTTC GATGTATTTC TAGAAACTGT GAGAGAGGCT 
GCAGCTGGGA ACATTGGGGG CCAGACAGTA AGAGAACGCT TGGCTCAGCG CCTCTGTCCC 
CCTGAGCTCC CCATTCCTGG TTCTGGGCTC CCGCCTCCAC GAAAGGTTCT AATCCTAGGC 
TCAGGGGGCC TCTCCATTGG CCAAGCTGGA GAATTTGACT ACTCGGGCTC TCAGGCAATT 
AAAGCCCTAA AGGAGGAAAA CATCCAGACA TTACTAATCA ACCCCAACAT TGCCACAGTG 
CAGACTTCTC AGGGGCTGGC AGACAAGGTC TATTTCCTTC CCATAACACT TCACTACGTA 
ACCCAGGTAA TTCGTAATGA ACGCCCAGAT GGTGTGTTAC TGACTTTTGG GGGTCAAACA 
GCCCTGAACT GCGGTGTGGA GCTGACCAAA GCTGGGGTAC TGGCTCGATA TGGGGTCCGG 
GTCTTGGGTA CACCCGTGGA GACCATTGAA CTGACTGAGG ACCGACGAGC CTTCGCTGCC 
AGGATGGCTG AGATCGGAGA GCATGTCGCC CCCAGTGAAG CGGCAAATTC TCTTGAACAG 
GCTCAGGCAG CTGCCGAGCG ACTAGGCTAC CCTGTGCTGG TGCGAGCAGC CTTTGCCCTG 
GGTGGTCTCG GTTCTGGCTT TGCTTCCACC AAAGAGGAAC TCTCAGCTCT TGTGGCTCCA 
GCTTTTGCCC ATACCAGTCA GGTGCTGATA GACAAGTCTC TGAAGGGCTG GAAGGAGATT 
GAATATGAGG TGGTGAGAGA CGCCTATGGC AACTGCGTAA CGGTATGCAA CATGGAGAAC 
TTAGACCCAC TGGGCATTCA TACTGGTGAA TCCATAGTGG TGGCTCCGAG CCAGACACTG 
AATGACAGAG AGTACCAGCT TCTGCGACGG ACAGCTATCA AGGTCACTCA GCACCTGGGC 
ATTGTCGGGG AGTGCAATGT ACAGTATGCT TTGAACCCCG AGTCTGAGCA GTATTACATC 
ATTGAAGTGA ATGCCAGGCT CTCCCGAAGC TCTGCCCTGG CCAGTAAGGC CACAGGCTAT 
CCACTAGCCT ATGTGGCAGC CAAGCTAGCA TTGGGCATTC CCCTGCCAGA GCTCAGAAAC 
TCTGTTACGG GAGGAACAGC AGCCTTTGAG CCCAGCCTGG ACTACTGCGT GGTAAAGATT 
CCTCGCTGGG ACCTCAGCAA GTTCCTACGT GTCAGTACGA AGATTGGGAG TTGCATGAAG 
AGTGTTGGCG AGGTCATGGG CATCGGGCGG TCCTTTGAGG AGGCCTTCCA AAAGGCCCTG 
CGCATGGTCG ATGAGAACTG CGTAGGCTTC GACCATACAG TAAAGCCAGT CAGCGACATG 
GAGCTGGAGA CTCCGACGGA TAAGCGGATC TTTGTGGTGG CTGCCGCTCT GTGGGCTGGC 
TACTCTGTGG AGCGCCTCTA CGAGCTCACC CGCATTGATT GCTGGTTCCT GCACCGGATG 
AAGCGCATTG TGACCCACGC CCAGTTGCTG GAACAACACC GTGGACAAGC TTTACCCCAA 
GACCTGCTGC ACCAGGCCAA GTGCCTCGGC TTCTCAGACA AGCAGATTGC CCTGGCAGTC 
CTGAGCACAG AGCTGGCTGT TCGGAAGCTG CGTCAGGAAC TGGGGATCTG CCCAGCGGTG 
AAACAGATTG ACACAGTTGC AGCTGAGTGG CCAGCCCAGA CAAATTACCT GTACCTGACA 
TACTGGGGCA ACACCCATGA CCTCGACTTT CGGGCACCTC ACGTCCTGGT CCTTGGCTCT 
GGTGTCTACC GGATTGGCTC CAGTGTTGAG TTTGATTGGT GTGCTGTGGG TTGCATCCAG 
CAGCTCCGGA AGATGGGTTA TAAGACCATC ATGGTGAACT ACAACCCAGA GACAGTCAGC 
ACCGACTATG ATATGTGTGA CCGACTCTAC TTTGATGAGA TCTCTTTTGA GGTGGTGATG 
GACATCTATG AGTTAGAGAA CCCCGAAGGT GTGATCCTGT CCATGGGTGG GCAGCTGCCC 
AACAACATGG CCATGGCTTT GCATCGGCAG CAGTGCCGAG TGCTGGGCAC CTCCCCCGAA 
GCCATCGACT CAGCCGAGAA CCGATTCAAG TTCTCCCGGC TCCTAGATAC CATCGGTATC 
AGCCAGCCTC AGTGGCGAGA GCTCAGTGAC CTTGAGTCTG CTCGCCAGTT CTGCCACACG 
GTCGGGTACC CCTGCGTAGT GCGCCCCTCC TATGTGCTCA GCGGTGCTGC TATGAATGTG 
GCGTACACCG ACGGGGACCT GGAGCGTTTC CTGAGCAGTG CGGCCGCCGT CTCTAAGGAG 
CACCCCGTAG TCATCTCCAA GTTCATTCAG GAAGCAAAGG AGATCGACGT GGACGCGGTG 
GCCTGTGACG GCATCGTGTC GGCCATTGCC ATCTCTGAGC ACGTGGAGAA TGCAGGTGTG 
CATTCAGGTG ATGCCACGCT GGTGACCCCC CCACAAGACA TCACCCCGAA AACTCTGGAG 
CGGATCAAAG CCATTGTGCA TGCTGTGGGC CAGGAGCTAC AGGTTACAGG ACCCTTCAAT 
CTGCAGCTCA TTGCCAAGGA TGACCAGCTG AAGGTTATTG AATGCAACGT GCGTGTATCT 
CGCTCTTTCC CCTTCGTGTC CAAGACACTG GGTGTTGATC TAGTAGCCTT GGCCACAAGG 
ATCATCATGG GGGAGAAAGT AGAGCCCGTG GGGCTCATGA CTGGCTCTGG AGTCGTGGGA 
GTAAAGGTAC CTCAGTTCTC CTTCTCACGC TTGGCCGGTG CCGACGTGGT ACTTGGTGTG 
GAGATGACCA GTACTGGAGA GGTGGCCGGC TTCGGAGAGA GCCGTTGTGA AGCCTACCTC 
AAAGCCATGC TCAGCACTGG CTTTAAGATC CCAGAGAAGA ACATCCTGCT GACTATTGGC 
AGCTACAAGA ACAAAAGTGA GCTGCTCCCA ACTGTGCGGT TGCTGGAGAG CCTGGGCTAC 
AGCCTCTATG CCAGCCTGGG TACGGCAGAC TTCTACACTG AGCACGGGGT CAAGGTAACA 
GCTGTGGACT GGCACTTTGA AGAGGCGGTG GATGGTGAGT GCCCACCACA ACGGAGCATC 
TTGGATCAGC TGGCTGAAAA TCACTTTGAG CTAGTGATTA ACCTGTCCAT GCGTGGGGCT 
GGGGGTCGGC GTCTTTCCTC CTTCGTTACC AAGGGCTACC GTACCCGGCG TCTGGCCGCT 
GATTTCTCTG TGCCTCTCAT CATTGACATC AAGTGCACCA AACTCTTTGT GGAGGCCCTA 
GGCCAGATCG GCCCAGCCCC TCCTTTGAAA GTGCACGTTG ACTGCATGAC CTCCCAGAAG 
CTAGTGCGCC TGCCCGGATT GATTGATGTC CACGTGCACC TTCGGGAACC AGGTGGGACA 
CACAAAGAGG ACTTTGCCTC AGGCACAGCT GCTGCCTTGG CTGGGGGTGT CACCATGGTT 
TGTGCTATGC CTAATACCCG ACCCCCCATC ATTGATGCTC CTGCTCTCGC CCTTGCCCAG 
AAGCTGGCAG AGGCTGGCGC CCGCTGTGAC TTTACCTTAT TCCTTGGAGC CTCATCTGAG 
AACGCGGGGA CTCTGGGTGC TGTGGCCGGC TCTGCAGCAG GCCTGAAGCT CTACCTCAAC 
GAGACCTTCT CTGAGCTGCG GCTGGACAGT GTGGCCCAGT GGATGGAGCA TTTTGAAACC 
TGGCCTGCCC ACCTCCCCAT TGTGGCCCAT GCAGAGCGGC AGAGTGTCGC TGCGGTCCTC 
ATGGTGGCTC AGCTGACCCA GCGTCCAGTG CACATATGTC ATGTGGCTCG GAAGGAAGAG 
ATCCTGTTGA TTAAAACTGC AAAGGCGCAG GGGCTGCCAG TGACCTGTGA GGTCGCTCCT 
CACCACCTGT TCCTAAACCG GGAAGACTTG GAGCGCCTGG GACCTGGGAA GGGAGAGGTC 
CGTCCTGAGC TTGGGTCCCG AGAGGATATG GAAGCCCTGT GGGAGAATAT GGCAGTCATC 
GACTGTTTTG CCTCAGACCA CGCTCCCCAT ACCTTGGAAG AGAAGTGTGG GCCCAAGCCT 
CCACCTGGCT TCCCGGGCCT AGAGACCATG TTGCCGCTGC TGCTGACAGC TGTGAGCGAG 
GGCCGCCTCA GTCTGGATGA CCTGCTGCAG CGATTGCACC ACAACCCTCG GCGGATCTTC 
CACCTGCCTC TCCAGGAGGA CACCTATGTA GAGGTGGATC TGGAGCATGA GTGGACAGTC 
CCCAGCCACA TGCCCTTCTC TAAGGCCCGC TGGACCCCAT TTGAAGGACA GAAGGTGAAG 
GGCACAGTGC GCCGTGTGGT CTTGCGAGGG GAGGTCGCCT ATATTGATGG ACAGGTGCTG 
GTACCCCCGG GCTATGGACA AGATGTACGG AAGTGGCCGC AGGGGGTTGT TCCCCAGCCC 
CCTCCTTCAA CTCCCGCCAC CACCGAAATC ACCACGACAC CTGAGCGACC ACGCCGAGTC 
ATCCCGGGCC TTCCTGATGG CCGCTTCCAC CTGCCACCCC GCATCCACCG GGCCTCTGAC 
CCAGGTCTGC CAGCTGAGGA ACCTAAAGAG AAGCCACCCA GGAAGGTAGT GGAACCAGAG 
CTGATGGGAA CCCCTGATGG TCCCTGCTAC CCTGCACCAC CAGTACCTAG ACAGGCATCA 
CCCCAGAACC TAGGGTCTTC TGGCCTGCTG CACCCCCAGA TGTCGCCCCT ACTGCACTCC 
CTAGTGGGCC AGCACATCCT GTCTGTCAAG CAGTTCACTA AGGATCAGAT GTCTCATCTG 
TTCAATGTGG CGCACACGCT ACGGATGATG GTACAGAAGG AGCGGAGCCT CGACATCCTG 
AAGCTGGCAG CCAAGCACTG TCGCAGGCCA GTGATCAATG CTGGGGATGG AGTTGGGGAG 
CACCCCACAC AGGCCCTGCT GGACATCTTC ACCATTCGAG AAGAGCTGGG GACTGTCAAT 
GGCATGACGA TCACTATGGT CGGTGACCTG AAGCATGGAC GCACAGTGCA CTCCCTGGCC 
TGCCTGCTCA CCCAGTACCG TGTGAGCCTA CGCTACGTGG CACCTCCCAG CCTGCGCATG 
CCACCCAGTG TACGGGACTT TGTGGCCTCC CGAGGCACCA AGCAGGAGGA GTTTGAGAGC 
ATCGAGGAGG CGCTGCCCGA CACAGACGTG CTCTACATGA CTCGGATCCA GAAAGAACGA 
TTTGGCTCTG TCCAGGAATA TGAAGCTTGC TTTGGTCAGT TCATCCTCAC TCCTCACATC 
ATGACCCGGG CCAAGAAGAA GATGGTGGTG ATGCACCCAA TGCCCCGTGT CAATGAGATA 
AGTGTGGAGG TGGACTCGGA CCCCCGGGCA GCCTACTTCC GCCAAGCTGA GAACGGCATG 
TACATCCGCA TGGCCCTGCT TGCCACCGTG CTTGGCCGTT TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001276451.1
CDS	176..6640
Translation	MAALVLEDGSVLQGRPFGAAVSTAGEVVFQTGMVGYPEALTDPSYKAQILVLTYPLIGNYGIPSDEEDEFGLSKWFESSEIHVAGLVVGECCPTPSHWSANCTLHEWLQQRGIPGLQGVDTRELTKKLREQGSLLGKLVQKGTEPSALPFVDPNARPLAPEVSIKTPRVFNAGGAPRICALDCGLKYNQIRCLCQLGAEVTVVPWDHELDSQKYDGLFLSNGPGDPASYPGVVSTLSRVLSEPNPRPVFGICLGHQLLALAIGAKTYKMRYGNRGHNQPCLLVGTGRCFLTSQNHGFAVDADSLPAGWAPLFTNANDCSNEGIVHDSLPFFSVQFHPEHRAGPSDMELLFDVFLETVREAAAGNIGGQTVRERLAQRLCPPELPIPGSGLPPPRKVLILGSGGLSIGQAGEFDYSGSQAIKALKEENIQTLLINPNIATVQTSQGLADKVYFLPITLHYVTQVIRNERPDGVLLTFGGQTALNCGVELTKAGVLARYGVRVLGTPVETIELTEDRRAFAARMAEIGEHVAPSEAANSLEQAQAAAERLGYPVLVRAAFALGGLGSGFASTKEELSALVAPAFAHTSQVLIDKSLKGWKEIEYEVVRDAYGNCVTVCNMENLDPLGIHTGESIVVAPSQTLNDREYQLLRRTAIKVTQHLGIVGECNVQYALNPESEQYYIIEVNARLSRSSALASKATGYPLAYVAAKLALGIPLPELRNSVTGGTAAFEPSLDYCVVKIPRWDLSKFLRVSTKIGSCMKSVGEVMGIGRSFEEAFQKALRMVDENCVGFDHTVKPVSDMELETPTDKRIFVVAAALWAGYSVERLYELTRIDCWFLHRMKRIVTHAQLLEQHRGQALPQDLLHQAKCLGFSDKQIALAVLSTELAVRKLRQELGICPAVKQIDTVAAEWPAQTNYLYLTYWGNTHDLDFRAPHVLVLGSGVYRIGSSVEFDWCAVGCIQQLRKMGYKTIMVNYNPETVSTDYDMCDRLYFDEISFEVVMDIYELENPEGVILSMGGQLPNNMAMALHRQQCRVLGTSPEAIDSAENRFKFSRLLDTIGISQPQWRELSDLESARQFCHTVGYPCVVRPSYVLSGAAMNVAYTDGDLERFLSSAAAVSKEHPVVISKFIQEAKEIDVDAVACDGIVSAIAISEHVENAGVHSGDATLVTPPQDITPKTLERIKAIVHAVGQELQVTGPFNLQLIAKDDQLKVIECNVRVSRSFPFVSKTLGVDLVALATRIIMGEKVEPVGLMTGSGVVGVKVPQFSFSRLAGADVVLGVEMTSTGEVAGFGESRCEAYLKAMLSTGFKIPEKNILLTIGSYKNKSELLPTVRLLESLGYSLYASLGTADFYTEHGVKVTAVDWHFEEAVDGECPPQRSILDQLAENHFELVINLSMRGAGGRRLSSFVTKGYRTRRLAADFSVPLIIDIKCTKLFVEALGQIGPAPPLKVHVDCMTSQKLVRLPGLIDVHVHLREPGGTHKEDFASGTAAALAGGVTMVCAMPNTRPPIIDAPALALAQKLAEAGARCDFTLFLGASSENAGTLGAVAGSAAGLKLYLNETFSELRLDSVAQWMEHFETWPAHLPIVAHAERQSVAAVLMVAQLTQRPVHICHVARKEEILLIKTAKAQGLPVTCEVAPHHLFLNREDLERLGPGKGEVRPELGSREDMEALWENMAVIDCFASDHAPHTLEEKCGPKPPPGFPGLETMLPLLLTAVSEGRLSLDDLLQRLHHNPRRIFHLPLQEDTYVEVDLEHEWTVPSHMPFSKARWTPFEGQKVKGTVRRVVLRGEVAYIDGQVLVPPGYGQDVRKWPQGVVPQPPPSTPATTEITTTPERPRRVIPGLPDGRFHLPPRIHRASDPGLPAEEPKEKPPRKVVEPELMGTPDGPCYPAPPVPRQASPQNLGSSGLLHPQMSPLLHSLVGQHILSVKQFTKDQMSHLFNVAHTLRMMVQKERSLDILKLAAKHCRRPVINAGDGVGEHPTQALLDIFTIREELGTVNGMTITMVGDLKHGRTVHSLACLLTQYRVSLRYVAPPSLRMPPSVRDFVASRGTKQEEFESIEEALPDTDVLYMTRIQKERFGSVQEYEACFGQFILTPHIMTRAKKKMVVMHPMPRVNEISVEVDSDPRAAYFRQAENGMYIRMALLATVLGRF

Target ORF information:

RefSeq Version	NM_001289522.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001289522.1

ORF Insert Sequence:

ATGGCGGCCC TGGTGTTGGA GGACGGGTCG GTGCTGCAGG GCCGGCCCTT TGGGGCCGCT 
GTGTCGACTG CTGGGGAAGT GGTGTTTCAG ACCGGCATGG TCGGCTACCC AGAGGCCCTC 
ACTGACCCTT CCTACAAAGC TCAGATCCTA GTGCTAACGT ATCCTCTCAT CGGCAACTAC 
GGCATTCCCT CAGATGAAGA GGACGAGTTC GGCCTGAGCA AGTGGTTTGA ATCCTCGGAG 
ATCCACGTGG CGGGACTTGT AGTGGGAGAA TGCTGTCCCA CACCCAGCCA CTGGAGTGCC 
AACTGCACCC TGCATGAGTG GCTGCAACAG CGTGGCATCC CCGGCCTGCA AGGAGTGGAC 
ACTCGGGAGC TGACTAAGAA GTTGCGGGAG CAAGGGTCTC TTTTGGGGAA GCTGGTCCAG 
AAAGGGACAG AGCCGTCAGC CCTGCCATTC GTGGACCCCA ATGCCAGGCC CCTGGCACCA 
GAGGTCTCTA TTAAGACTCC ACGGGTATTC AATGCAGGGG GTGCCCCTCG GATCTGTGCT 
CTGGATTGTG GCCTCAAGTA TAATCAGATC CGATGTCTCT GCCAGCTTGG GGCTGAAGTT 
ACCGTGGTAC CCTGGGACCA CGAGTTAGAC AGTCAGAAAT ATGACGGTCT CTTCCTAAGT 
AATGGACCCG GTGACCCTGC CTCTTACCCG GGTGTGGTGT CCACACTGAG CCGAGTCTTG 
TCTGAACCTA ACCCCCGACC TGTGTTTGGA ATCTGCCTTG GACACCAGCT GTTGGCTTTA 
GCCATCGGGG CCAAAACTTA CAAGATGAGA TACGGAAACC GAGGCCACAA CCAGCCCTGT 
TTACTGGTGG GCACTGGGCG CTGCTTTCTG ACGTCCCAGA ATCATGGGTT TGCAGTGGAC 
GCAGATTCGC TGCCAGCAGG CTGGGCTCCT CTGTTCACCA ATGCCAATGA CTGTTCCAAT 
GAAGGCATAG TGCACGACAG CCTGCCCTTC TTCAGTGTCC AGTTTCACCC AGAGCACCGG 
GCCGGCCCTT CAGATATGGA ACTGCTCTTC GATGTATTTC TAGAAACTGT GAGAGAGGCT 
GCAGCTGGGA ACATTGGGGG CCAGACAGTA AGAGAACGCT TGGCTCAGCG CCTCTGTCCC 
CCTGAGCTCC CCATTCCTGG TTCTGGGCTC CCGCCTCCAC GAAAGGTTCT AATCCTAGGC 
TCAGGGGGCC TCTCCATTGG CCAAGCTGGA GAATTTGACT ACTCGGGCTC TCAGGCAATT 
AAAGCCCTAA AGGAGGAAAA CATCCAGACA TTACTAATCA ACCCCAACAT TGCCACAGTG 
CAGACTTCTC AGGGGCTGGC AGACAAGGTC TATTTCCTTC CCATAACACT TCACTACGTA 
ACCCAGGTAA TTCGTAATGA ACGCCCAGAT GGTGTGTTAC TGACTTTTGG GGGTCAAACA 
GCCCTGAACT GCGGTGTGGA GCTGACCAAA GCTGGGGTAC TGGCTCGATA TGGGGTCCGG 
GTCTTGGGTA CACCCGTGGA GACCATTGAA CTGACTGAGG ACCGACGAGC CTTCGCTGCC 
AGGATGGCTG AGATCGGAGA GCATGTCGCC CCCAGTGAAG CGGCAAATTC TCTTGAACAG 
GCTCAGGCAG CTGCCGAGCG ACTAGGCTAC CCTGTGCTGG TGCGAGCAGC CTTTGCCCTG 
GGTGGTCTCG GTTCTGGCTT TGCTTCCACC AAAGAGGAAC TCTCAGCTCT TGTGGCTCCA 
GCTTTTGCCC ATACCAGTCA GGTGCTGATA GACAAGTCTC TGAAGGGCTG GAAGGAGATT 
GAATATGAGG TGGTGAGAGA CGCCTATGGC AACTGCGTAA CGGTATGCAA CATGGAGAAC 
TTAGACCCAC TGGGCATTCA TACTGGTGAA TCCATAGTGG TGGCTCCGAG CCAGACACTG 
AATGACAGAG AGTACCAGCT TCTGCGACGG ACAGCTATCA AGGTCACTCA GCACCTGGGC 
ATTGTCGGGG AGTGCAATGT ACAGTATGCT TTGAACCCCG AGTCTGAGCA GTATTACATC 
ATTGAAGTGA ATGCCAGGCT CTCCCGAAGC TCTGCCCTGG CCAGTAAGGC CACAGGCTAT 
CCACTAGCCT ATGTGGCAGC CAAGCTAGCA TTGGGCATTC CCCTGCCAGA GCTCAGAAAC 
TCTGTTACGG GAGGAACAGC AGCCTTTGAG CCCAGCCTGG ACTACTGCGT GGTAAAGATT 
CCTCGCTGGG ACCTCAGCAA GTTCCTACGT GTCAGTACGA AGATTGGGAG TTGCATGAAG 
AGTGTTGGCG AGGTCATGGG CATCGGGCGG TCCTTTGAGG AGGCCTTCCA AAAGGCCCTG 
CGCATGGTCG ATGAGAACTG CGTAGGCTTC GACCATACAG TAAAGCCAGT CAGCGACATG 
GAGCTGGAGA CTCCGACGGA TAAGCGGATC TTTGTGGTGG CTGCCGCTCT GTGGGCTGGC 
TACTCTGTGG AGCGCCTCTA CGAGCTCACC CGCATTGATT GCTGGTTCCT GCACCGGATG 
AAGCGCATTG TGACCCACGC CCAGTTGCTG GAACAACACC GTGGACAAGC TTTACCCCAA 
GACCTGCTGC ACCAGGCCAA GTGCCTCGGC TTCTCAGACA AGCAGATTGC CCTGGCAGTC 
CTGAGCACAG AGCTGGCTGT TCGGAAGCTG CGTCAGGAAC TGGGGATCTG CCCAGCGGTG 
AAACAGATTG ACACAGTTGC AGCTGAGTGG CCAGCCCAGA CAAATTACCT GTACCTGACA 
TACTGGGGCA ACACCCATGA CCTCGACTTT CGGGCACCTC ACGTCCTGGT CCTTGGCTCT 
GGTGTCTACC GGATTGGCTC CAGTGTTGAG TTTGATTGGT GTGCTGTGGG TTGCATCCAG 
CAGCTCCGGA AGATGGGTTA TAAGACCATC ATGGTGAACT ACAACCCAGA GACAGTCAGC 
ACCGACTATG ATATGTGTGA CCGACTCTAC TTTGATGAGA TCTCTTTTGA GGTGGTGATG 
GACATCTATG AGTTAGAGAA CCCCGAAGGT GTGATCCTGT CCATGGGTGG GCAGCTGCCC 
AACAACATGG CCATGGCTTT GCATCGGCAG CAGTGCCGAG TGCTGGGCAC CTCCCCCGAA 
GCCATCGACT CAGCCGAGAA CCGATTCAAG TTCTCCCGGC TCCTAGATAC CATCGGTATC 
AGCCAGCCTC AGTGGCGAGA GCTCAGTGAC CTTGAGTCTG CTCGCCAGTT CTGCCACACG 
GTCGGGTACC CCTGCGTAGT GCGCCCCTCC TATGTGCTCA GCGGTGCTGC TATGAATGTG 
GCGTACACCG ACGGGGACCT GGAGCGTTTC CTGAGCAGTG CGGCCGCCGT CTCTAAGGAG 
CACCCCGTAG TCATCTCCAA GTTCATTCAG GAAGCAAAGG AGATCGACGT GGACGCGGTG 
GCCTGTGACG GCATCGTGTC GGCCATTGCC ATCTCTGAGC ACGTGGAGAA TGCAGGTGTG 
CATTCAGGTG ATGCCACGCT GGTGACCCCC CCACAAGACA TCACCCCGAA AACTCTGGAG 
CGGATCAAAG CCATTGTGCA TGCTGTGGGC CAGGAGCTAC AGGTTACAGG ACCCTTCAAT 
CTGCAGCTCA TTGCCAAGGA TGACCAGCTG AAGGTTATTG AATGCAACGT GCGTGTATCT 
CGCTCTTTCC CCTTCGTGTC CAAGACACTG GGTGTTGATC TAGTAGCCTT GGCCACAAGG 
ATCATCATGG GGGAGAAAGT AGAGCCCGTG GGGCTCATGA CTGGCTCTGG AGTCGTGGGA 
GTAAAGGTAC CTCAGTTCTC CTTCTCACGC TTGGCCGGTG CCGACGTGGT ACTTGGTGTG 
GAGATGACCA GTACTGGAGA GGTGGCCGGC TTCGGAGAGA GCCGTTGTGA AGCCTACCTC 
AAAGCCATGC TCAGCACTGG CTTTAAGATC CCAGAGAAGA ACATCCTGCT GACTATTGGC 
AGCTACAAGA ACAAAAGTGA GCTGCTCCCA ACTGTGCGGT TGCTGGAGAG CCTGGGCTAC 
AGCCTCTATG CCAGCCTGGG TACGGCAGAC TTCTACACTG AGCACGGGGT CAAGGTAACA 
GCTGTGGACT GGCACTTTGA AGAGGCGGTG GATGGTGAGT GCCCACCACA ACGGAGCATC 
TTGGATCAGC TGGCTGAAAA TCACTTTGAG CTAGTGATTA ACCTGTCCAT GCGTGGGGCT 
GGGGGTCGGC GTCTTTCCTC CTTCGTTACC AAGGGCTACC GTACCCGGCG TCTGGCCGCT 
GATTTCTCTG TGCCTCTCAT CATTGACATC AAGTGCACCA AACTCTTTGT GGAGGCCCTA 
GGCCAGATCG GCCCAGCCCC TCCTTTGAAA GTGCACGTTG ACTGCATGAC CTCCCAGAAG 
CTAGTGCGCC TGCCCGGATT GATTGATGTC CACGTGCACC TTCGGGAACC AGGTGGGACA 
CACAAAGAGG ACTTTGCCTC AGGCACAGCT GCTGCCTTGG CTGGGGGTGT CACCATGGTT 
TGTGCTATGC CTAATACCCG ACCCCCCATC ATTGATGCTC CTGCTCTCGC CCTTGCCCAG 
AAGCTGGCAG AGGCTGGCGC CCGCTGTGAC TTTACCTTAT TCCTTGGAGC CTCATCTGAG 
AACGCGGGGA CTCTGGGTGC TGTGGCCGGC TCTGCAGCAG GCCTGAAGCT CTACCTCAAC 
GAGACCTTCT CTGAGCTGCG GCTGGACAGT GTGGCCCAGT GGATGGAGCA TTTTGAAACC 
TGGCCTGCCC ACCTCCCCAT TGTGGCCCAT GCAGAGCGGC AGAGTGTCGC TGCGGTCCTC 
ATGGTGGCTC AGCTGACCCA GCGTCCAGTG CACATATGTC ATGTGGCTCG GAAGGAAGAG 
ATCCTGTTGA TTAAAACTGC AAAGGCGCAG GGGCTGCCAG TGACCTGTGA GGTCGCTCCT 
CACCACCTGT TCCTAAACCG GGAAGACTTG GAGCGCCTGG GACCTGGGAA GGGAGAGGTC 
CGTCCTGAGC TTGGGTCCCG AGAGGATATG GAAGCCCTGT GGGAGAATAT GGCAGTCATC 
GACTGTTTTG CCTCAGACCA CGCTCCCCAT ACCTTGGAAG AGAAGTGTGG GCCCAAGCCT 
CCACCTGGCT TCCCGGGCCT AGAGACCATG TTGCCGCTGC TGCTGACAGC TGTGAGCGAG 
GGCCGCCTCA GTCTGGATGA CCTGCTGCAG CGATTGCACC ACAACCCTCG GCGGATCTTC 
CACCTGCCTC TCCAGGAGGA CACCTATGTA GAGGTGGATC TGGAGCATGA GTGGACAGTC 
CCCAGCCACA TGCCCTTCTC TAAGGCCCGC TGGACCCCAT TTGAAGGACA GAAGGTGAAG 
GGCACAGTGC GCCGTGTGGT CTTGCGAGGG GAGGTCGCCT ATATTGATGG ACAGGTGCTG 
GTACCCCCGG GCTATGGACA AGATGTACGG AAGTGGCCGC AGGGGGTTGT TCCCCAGCCC 
CCTCCTTCAA CTCCCGCCAC CACCGAAATC ACCACGACAC CTGAGCGACC ACGCCGAGTC 
ATCCCGGGCC TTCCTGATGG CCGCTTCCAC CTGCCACCCC GCATCCACCG GGCCTCTGAC 
CCAGGTCTGC CAGCTGAGGA ACCTAAAGAG AAGCCACCCA GGAAGGTAGT GGAACCAGAG 
CTGATGGGAA CCCCTGATGG TCCCTGCTAC CCTGCACCAC CAGTACCTAG ACAGGCATCA 
CCCCAGAACC TAGGGTCTTC TGGCCTGCTG CACCCCCAGA TGTCGCCCCT ACTGCACTCC 
CTAGTGGGCC AGCACATCCT GTCTGTCAAG CAGTTCACTA AGGATCAGAT GTCTCATCTG 
TTCAATGTGG CGCACACGCT ACGGATGATG GTACAGAAGG AGCGGAGCCT CGACATCCTG 
AAGCTGGCAG CCAAGCACTG TCGCAGGCCA GTGATCAATG CTGGGGATGG AGTTGGGGAG 
CACCCCACAC AGGCCCTGCT GGACATCTTC ACCATTCGAG AAGAGCTGGG GACTGTCAAT 
GGCATGACGA TCACTATGGT CGGTGACCTG AAGCATGGAC GCACAGTGCA CTCCCTGGCC 
TGCCTGCTCA CCCAGTACCG TGTGAGCCTA CGCTACGTGG CACCTCCCAG CCTGCGCATG 
CCACCCAGTG TACGGGACTT TGTGGCCTCC CGAGGCACCA AGCAGGAGGA GTTTGAGAGC 
ATCGAGGAGG CGCTGCCCGA CACAGACGTG CTCTACATGA CTCGGATCCA GAAAGAACGA 
TTTGGCTCTG TCCAGGAATA TGAAGCTTGC TTTGGTCAGT TCATCCTCAC TCCTCACATC 
ATGACCCGGG CCAAGAAGAA GATGGTGGTG ATGCACCCAA TGCCCCGTGT CAATGAGATA 
AGTGTGGAGG TGGACTCGGA CCCCCGGGCA GCCTACTTCC GCCAAGCTGA GAACGGCATG 
TACATCCGCA TGGCCCTGCT TGCCACCGTG CTTGGCCGTT TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cad ( XM_006504091.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006504154.1 Mus musculus carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad), transcript variant X1, mRNA.

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CloneID	OMu35179
Clone ID Related Accession (Same CDS sequence)	XM_006504091.3 , XM_006504091.4
Accession Version	XM_006504091.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6966bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	CAD protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006504091.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCCGC CCCTGACGTG CCCGGCCCCG CCCCTGACGT TGCCGGCTGC TTGCGCCGTC 
GCAGTCGTGC TTCAGCTCAC TACGCTTAGG GCTCTGGCTT GCCGCTCCCG CCTGCTCTCC 
AGCGCCCCGC GCAGCGAGCC ACGTGGACCA ACTCCGGCGC GCGGTGTTCG CTTGGTTCCA 
GTGGGGCTCG CCGCGCCTCC CGCGTCTGCG TGCTTGCCCT GTCTCAGCTC CGACCCGATG 
GCGGCCCTGG TGTTGGAGGA CGGGTCGGTG CTGCAGGGCC GGCCCTTTGG GGCCGCTGTG 
TCGACTGCTG GGGAAGTGGT GTTTCAGACC GGCATGGTCG GCTACCCAGA GGCCCTCACT 
GACCCTTCCT ACAAAGCTCA GATCCTAGTG CTAACGTATC CTCTCATCGG CAACTACGGC 
ATTCCCTCAG ATGAAGAGGA CGAGTTCGGC CTGAGCAAGT GGTTTGAATC CTCGGAGATC 
CACGTGGCGG GACTTGTAGT GGGAGAATGC TGTCCCACAC CCAGCCACTG GAGTGCCAAC 
TGCACCCTGC ATGAGTGGCT GCAACAGCGT GGCATCCCCG GCCTGCAAGG AGTGGACACT 
CGGGAGCTGA CTAAGAAGTT GCGGGAGCAA GGGTCTCTTT TGGGGAAGCT GGTCCAGAAA 
GGGACAGAGC CGTCAGCCCT GCCATTCGTG GACCCCAATG CCAGGCCCCT GGCACCAGAG 
GTCTCTATTA AGACTCCACG GGTATTCAAT GCAGGGGGTG CCCCTCGGAT CTGTGCTCTG 
GATTGTGGCC TCAAGTATAA TCAGATCCGA TGTCTCTGCC AGCTTGGGGC TGAAGTTACC 
GTGGTACCCT GGGACCACGA GTTAGACAGT CAGAAATATG ACGGTCTCTT CCTAAGTAAT 
GGACCCGGTG ACCCTGCCTC TTACCCGGGT GTGGTGTCCA CACTGAGCCG AGTCTTGTCT 
GAACCTAACC CCCGACCTGT GTTTGGAATC TGCCTTGGAC ACCAGCTGTT GGCTTTAGCC 
ATCGGGGCCA AAACTTACAA GATGAGATAC GGAAACCGAG GCCACAACCA GCCCTGTTTA 
CTGGTGGGCA CTGGGCGCTG CTTTCTGACG TCCCAGAATC ATGGGTTTGC AGTGGACGCA 
GATTCGCTGC CAGCAGGCTG GGCTCCTCTG TTCACCAATG CCAATGACTG TTCCAATGAA 
GGCATAGTGC ACGACAGCCT GCCCTTCTTC AGTGTCCAGT TTCACCCAGA GCACCGGGCC 
GGCCCTTCAG ATATGGAACT GCTCTTCGAT GTATTTCTAG AAACTGTGAG AGAGGCTGCA 
GCTGGGAACA TTGGGGGCCA GACAGTAAGA GAACGCTTGG CTCAGCGCCT CTGTCCCCCT 
GAGCTCCCCA TTCCTGGTTC TGGGCTCCCG CCTCCACGAA AGGTTCTAAT CCTAGGCTCA 
GGGGGCCTCT CCATTGGCCA AGCTGGAGAA TTTGACTACT CGGGCTCTCA GGCAATTAAA 
GCCCTAAAGG AGGAAAACAT CCAGACATTA CTAATCAACC CCAACATTGC CACAGTGCAG 
ACTTCTCAGG GGCTGGCAGA CAAGGTCTAT TTCCTTCCCA TAACACTTCA CTACGTAACC 
CAGGTAATTC GTAATGAACG CCCAGATGGT GTGTTACTGA CTTTTGGGGG TCAAACAGCC 
CTGAACTGCG GTGTGGAGCT GACCAAAGCT GGGGTACTGG CTCGATATGG GGTCCGGGTC 
TTGGGTACAC CCGTGGAGAC CATTGAACTG ACTGAGGACC GACGAGCCTT CGCTGCCAGG 
ATGGCTGAGA TCGGAGAGCA TGTCGCCCCC AGTGAAGCGG CAAATTCTCT TGAACAGGCT 
CAGGCAGCTG CCGAGCGACT AGGCTACCCT GTGCTGGTGC GAGCAGCCTT TGCCCTGGGT 
GGTCTCGGTT CTGGCTTTGC TTCCACCAAA GAGGAACTCT CAGCTCTTGT GGCTCCAGCT 
TTTGCCCATA CCAGTCAGGT GCTGATAGAC AAGTCTCTGA AGGGCTGGAA GGAGATTGAA 
TATGAGGTGG TGAGAGACGC CTATGGCAAC TGCGTAACGG TATGCAACAT GGAGAACTTA 
GACCCACTGG GCATTCATAC TGGTGAATCC ATAGTGGTGG CTCCGAGCCA GACACTGAAT 
GACAGAGAGT ACCAGCTTCT GCGACGGACA GCTATCAAGG TCACTCAGCA CCTGGGCATT 
GTCGGGGAGT GCAATGTACA GTATGCTTTG AACCCCGAGT CTGAGCAGTA TTACATCATT 
GAAGTGAATG CCAGGCTCTC CCGAAGCTCT GCCCTGGCCA GTAAGGCCAC AGGCTATCCA 
CTAGCCTATG TGGCAGCCAA GCTAGCATTG GGCATTCCCC TGCCAGAGCT CAGAAACTCT 
GTTACGGGAG GAACAGCAGC CTTTGAGCCC AGCCTGGACT ACTGCGTGGT AAAGATTCCT 
CGCTGGGACC TCAGCAAGTT CCTACGTGTC AGTACGAAGA TTGGGAGTTG CATGAAGAGT 
GTTGGCGAGG TCATGGGCAT CGGGCGGTCC TTTGAGGAGG CCTTCCAAAA GGCCCTGCGC 
ATGGTCGATG AGAACTGCGT AGGCTTCGAC CATACAGTAA AGCCAGTCAG CGACATGGAG 
CTGGAGACTC CGACGGATAA GCGGATCTTT GTGGTGGCTG CCGCTCTGTG GGCTGGCTAC 
TCTGTGGAGC GCCTCTACGA GCTCACCCGC ATTGATTGCT GGTTCCTGCA CCGGATGAAG 
CGCATTGTGA CCCACGCCCA GTTGCTGGAA CAACACCGTG GACAAGCTTT ACCCCAAGAC 
CTGCTGCACC AGGCCAAGTG CCTCGGCTTC TCAGACAAGC AGATTGCCCT GGCAGTCCTG 
AGCACAGAGC TGGCTGTTCG GAAGCTGCGT CAGGAACTGG GGATCTGCCC AGCGGTGAAA 
CAGATTGACA CAGTTGCAGC TGAGTGGCCA GCCCAGACAA ATTACCTGTA CCTGACATAC 
TGGGGCAACA CCCATGACCT CGACTTTCGG GCACCTCACG TCCTGGTCCT TGGCTCTGGT 
GTCTACCGGA TTGGCTCCAG TGTTGAGTTT GATTGGTGTG CTGTGGGTTG CATCCAGCAG 
CTCCGGAAGA TGGGTTATAA GACCATCATG GTGAACTACA ACCCAGAGAC AGTCAGCACC 
GACTATGATA TGTGTGACCG ACTCTACTTT GATGAGATCT CTTTTGAGGT GGTGATGGAC 
ATCTATGAGT TAGAGAACCC CGAAGGTGTG ATCCTGTCCA TGGGTGGGCA GCTGCCCAAC 
AACATGGCCA TGGCTTTGCA TCGGCAGCAG TGCCGAGTGC TGGGCACCTC CCCCGAAGCC 
ATCGACTCAG CCGAGAACCG ATTCAAGTTC TCCCGGCTCC TAGATACCAT CGGTATCAGC 
CAGCCTCAGT GGCGAGAGCT CAGTGACCTT GAGTCTGCTC GCCAGTTCTG CCACACGGTC 
GGGTACCCCT GCGTAGTGCG CCCCTCCTAT GTGCTCAGCG GTGCTGCTAT GAATGTGGCG 
TACACCGACG GGGACCTGGA GCGTTTCCTG AGCAGTGCGG CCGCCGTCTC TAAGGAGCAC 
CCCGTAGTCA TCTCCAAGTT CATTCAGGAA GCAAAGGAGA TCGACGTGGA CGCGGTGGCC 
TGTGACGGCA TCGTGTCGGC CATTGCCATC TCTGAGCACG TGGAGAATGC AGGTGTGCAT 
TCAGGTGATG CCACGCTGGT GACCCCCCCA CAAGACATCA CCCCGAAAAC TCTGGAGCGG 
ATCAAAGCCA TTGTGCATGC TGTGGGCCAG GAGCTACAGG TTACAGGACC CTTCAATCTG 
CAGCTCATTG CCAAGGATGA CCAGCTGAAG GTTATTGAAT GCAACGTGCG TGTATCTCGC 
TCTTTCCCCT TCGTGTCCAA GACACTGGGT GTTGATCTAG TAGCCTTGGC CACAAGGATC 
ATCATGGGGG AGAAAGTAGA GCCCGTGGGG CTCATGACTG GCTCTGGAGT CGTGGGAGTA 
AAGGTACCTC AGTTCTCCTT CTCACGCTTG GCCGGTGCCG ACGTGGTACT TGGTGTGGAG 
ATGACCAGTA CTGGAGAGGT GGCCGGCTTC GGAGAGAGCC GTTGTGAAGC CTACCTCAAA 
GCCATGCTCA GCACTGGCTT TAAGATCCCA GAGAAGAACA TCCTGCTGAC TATTGGCAGC 
TACAAGAACA AAAGTGAGCT GCTCCCAACT GTGCGGTTGC TGGAGAGCCT GGGCTACAGC 
CTCTATGCCA GCCTGGGTAC GGCAGACTTC TACACTGAGC ACGGGGTCAA GGTAACAGCT 
GTGGACTGGC ACTTTGAAGA GGCGGTGGAT GGTGAGTGCC CACCACAACG GAGCATCTTG 
GATCAGCTGG CTGAAAATCA CTTTGAGCTA GTGATTAACC TGTCCATGCG TGGGGCTGGG 
GGTCGGCGTC TTTCCTCCTT CGTTACCAAG GGCTACCGTA CCCGGCGTCT GGCCGCTGAT 
TTCTCTGTGC CTCTCATCAT TGACATCAAG TGCACCAAAC TCTTTGTGGA GGCCCTAGGC 
CAGATCGGCC CAGCCCCTCC TTTGAAAGTG CACGTTGACT GCATGACCTC CCAGAAGCTA 
GTGCGCCTGC CCGGATTGAT TGATGTCCAC GTGCACCTTC GGGAACCAGG TGGGACACAC 
AAAGAGGACT TTGCCTCAGG CACAGCTGCT GCCTTGGCTG GGGGTGTCAC CATGGTTTGT 
GCTATGCCTA ATACCCGACC CCCCATCATT GATGCTCCTG CTCTCGCCCT TGCCCAGAAG 
CTGGCAGAGG CTGGCGCCCG CTGTGACTTT ACCTTATTCC TTGGAGCCTC ATCTGAGAAC 
GCGGGGACTC TGGGTGCTGT GGCCGGCTCT GCAGCAGGCC TGAAGCTCTA CCTCAACGAG 
ACCTTCTCTG AGCTGCGGCT GGACAGTGTG GCCCAGTGGA TGGAGCATTT TGAAACCTGG 
CCTGCCCACC TCCCCATTGT GGCCCATGCA GAGCGGCAGA GTGTCGCTGC GGTCCTCATG 
GTGGCTCAGC TGACCCAGCG TCCAGTGCAC ATATGTCATG TGGCTCGGAA GGAAGAGATC 
CTGTTGATTA AAACTGCAAA GGCGCAGGGG CTGCCAGTGA CCTGTGAGGT CGCTCCTCAC 
CACCTGTTCC TAAACCGGGA AGACTTGGAG CGCCTGGGAC CTGGGAAGGG AGAGGTCCGT 
CCTGAGCTTG GGTCCCGAGA GGATATGGAA GCCCTGTGGG AGAATATGGC AGTCATCGAC 
TGTTTTGCCT CAGACCACGC TCCCCATACC TTGGAAGAGA AGTGTGGGCC CAAGCCTCCA 
CCTGGCTTCC CGGGCCTAGA GACCATGTTG CCGCTGCTGC TGACAGCTGT GAGCGAGGGC 
CGCCTCAGTC TGGATGACCT GCTGCAGCGA TTGCACCACA ACCCTCGGCG GATCTTCCAC 
CTGCCTCTCC AGGAGGACAC CTATGTAGAG GTGGATCTGG AGCATGAGTG GACAGTCCCC 
AGCCACATGC CCTTCTCTAA GGCCCGCTGG ACCCCATTTG AAGGACAGAA GGTGAAGGGC 
ACAGTGCGCC GTGTGGTCTT GCGAGGGGAG GTCGCCTATA TTGATGGACA GGTGCTGGTA 
CCCCCGGGCT ATGGACAAGA TGTACGGAAG TGGCCGCAGG GGGTTGTTCC CCAGCCCCCT 
CCTTCAACTC CCGCCACCAC CGAAATCACC ACGACACCTG AGCGACCACG CCGAGTCATC 
CCGGGCCTTC CTGATGGCCG CTTCCACCTG CCACCCCGCA TCCACCGGGC CTCTGACCCA 
GGTCTGCCAG CTCTGTACCT CCGCCCAGGA GCTGGGATCT CATGGGGCAG CAGAGCATGG 
GCTGAGGAAC CTAAAGAGAA GCCACCCAGG AAGGTAGTGG AACCAGAGCT GATGGGAACC 
CCTGATGGTC CCTGCTACCC TGCACCACCA GTACCTAGAC AGGCATCACC CCAGAACCTA 
GGGTCTTCTG GCCTGCTGCA CCCCCAGATG TCGCCCCTAC TGCACTCCCT AGTGGGCCAG 
CACATCCTGT CTGTCAAGCA GTTCACTAAG GATCAGATGT CTCATCTGTT CAATGTGGCG 
CACACGCTAC GGATGATGGT ACAGAAGGAG CGGAGCCTCG ACATCCTGAA GGGGAAGGTC 
ATGGCCTCCA TGTTCTACGA GGTGAGCACC CGCACCAGTA GCTCCTTCGC AGCAGCCATG 
GCCCGGCTCG GGGGCGCTGT TCTCAGCTTC TCAGAAGCTA CATCCTCCGT CCAAAAGGGC 
GAGTCCCTTG CTGACTCGGT GCAGACCATG AGTTGCTATG CTGATGTCAT TGTGCTCCGA 
CACCCTCAGC CTGGAGCCGT GGAGCTGGCA GCCAAGCACT GTCGCAGGCC AGTGATCAAT 
GCTGGGGATG GAGTTGGGGA GCACCCCACA CAGGCCCTGC TGGACATCTT CACCATTCGA 
GAAGAGCTGG GGACTGTCAA TGGCATGACG ATCACTATGG TCGGTGACCT GAAGCATGGA 
CGCACAGTGC ACTCCCTGGC CTGCCTGCTC ACCCAGTACC GTGTGAGCCT ACGCTACGTG 
GCACCTCCCA GCCTGCGCAT GCCACCCAGT GTACGGGACT TTGTGGCCTC CCGAGGCACC 
AAGCAGGAGG AGTTTGAGAG CATCGAGGAG GCGCTGCCCG ACACAGACGT GCTCTACATG 
ACTCGGATCC AGAAAGAACG ATTTGGCTCT GTCCAGGAAT ATGAAGCTTG CTTTGGTCAG 
TTCATCCTCA CTCCTCACAT CATGACCCGG GCCAAGAAGA AGATGGTGGT GATGCACCCA 
ATGCCCCGTG TCAATGAGAT AAGTGTGGAG GTGGACTCGG ACCCCCGGGC AGCCTACTTC 
CGCCAAGCTG AGAACGGCAT GTACATCCGC ATGGCCCTGC TTGCCACCGT GCTTGGCCGT 
TTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006504154.1
CDS	23..6988
Translation	MVPPLTCPAPPLTLPAACAVAVVLQLTTLRALACRSRLLSSAPRSEPRGPTPARGVRLVPVGLAAPPASACLPCLSSDPMAALVLEDGSVLQGRPFGAAVSTAGEVVFQTGMVGYPEALTDPSYKAQILVLTYPLIGNYGIPSDEEDEFGLSKWFESSEIHVAGLVVGECCPTPSHWSANCTLHEWLQQRGIPGLQGVDTRELTKKLREQGSLLGKLVQKGTEPSALPFVDPNARPLAPEVSIKTPRVFNAGGAPRICALDCGLKYNQIRCLCQLGAEVTVVPWDHELDSQKYDGLFLSNGPGDPASYPGVVSTLSRVLSEPNPRPVFGICLGHQLLALAIGAKTYKMRYGNRGHNQPCLLVGTGRCFLTSQNHGFAVDADSLPAGWAPLFTNANDCSNEGIVHDSLPFFSVQFHPEHRAGPSDMELLFDVFLETVREAAAGNIGGQTVRERLAQRLCPPELPIPGSGLPPPRKVLILGSGGLSIGQAGEFDYSGSQAIKALKEENIQTLLINPNIATVQTSQGLADKVYFLPITLHYVTQVIRNERPDGVLLTFGGQTALNCGVELTKAGVLARYGVRVLGTPVETIELTEDRRAFAARMAEIGEHVAPSEAANSLEQAQAAAERLGYPVLVRAAFALGGLGSGFASTKEELSALVAPAFAHTSQVLIDKSLKGWKEIEYEVVRDAYGNCVTVCNMENLDPLGIHTGESIVVAPSQTLNDREYQLLRRTAIKVTQHLGIVGECNVQYALNPESEQYYIIEVNARLSRSSALASKATGYPLAYVAAKLALGIPLPELRNSVTGGTAAFEPSLDYCVVKIPRWDLSKFLRVSTKIGSCMKSVGEVMGIGRSFEEAFQKALRMVDENCVGFDHTVKPVSDMELETPTDKRIFVVAAALWAGYSVERLYELTRIDCWFLHRMKRIVTHAQLLEQHRGQALPQDLLHQAKCLGFSDKQIALAVLSTELAVRKLRQELGICPAVKQIDTVAAEWPAQTNYLYLTYWGNTHDLDFRAPHVLVLGSGVYRIGSSVEFDWCAVGCIQQLRKMGYKTIMVNYNPETVSTDYDMCDRLYFDEISFEVVMDIYELENPEGVILSMGGQLPNNMAMALHRQQCRVLGTSPEAIDSAENRFKFSRLLDTIGISQPQWRELSDLESARQFCHTVGYPCVVRPSYVLSGAAMNVAYTDGDLERFLSSAAAVSKEHPVVISKFIQEAKEIDVDAVACDGIVSAIAISEHVENAGVHSGDATLVTPPQDITPKTLERIKAIVHAVGQELQVTGPFNLQLIAKDDQLKVIECNVRVSRSFPFVSKTLGVDLVALATRIIMGEKVEPVGLMTGSGVVGVKVPQFSFSRLAGADVVLGVEMTSTGEVAGFGESRCEAYLKAMLSTGFKIPEKNILLTIGSYKNKSELLPTVRLLESLGYSLYASLGTADFYTEHGVKVTAVDWHFEEAVDGECPPQRSILDQLAENHFELVINLSMRGAGGRRLSSFVTKGYRTRRLAADFSVPLIIDIKCTKLFVEALGQIGPAPPLKVHVDCMTSQKLVRLPGLIDVHVHLREPGGTHKEDFASGTAAALAGGVTMVCAMPNTRPPIIDAPALALAQKLAEAGARCDFTLFLGASSENAGTLGAVAGSAAGLKLYLNETFSELRLDSVAQWMEHFETWPAHLPIVAHAERQSVAAVLMVAQLTQRPVHICHVARKEEILLIKTAKAQGLPVTCEVAPHHLFLNREDLERLGPGKGEVRPELGSREDMEALWENMAVIDCFASDHAPHTLEEKCGPKPPPGFPGLETMLPLLLTAVSEGRLSLDDLLQRLHHNPRRIFHLPLQEDTYVEVDLEHEWTVPSHMPFSKARWTPFEGQKVKGTVRRVVLRGEVAYIDGQVLVPPGYGQDVRKWPQGVVPQPPPSTPATTEITTTPERPRRVIPGLPDGRFHLPPRIHRASDPGLPALYLRPGAGISWGSRAWAEEPKEKPPRKVVEPELMGTPDGPCYPAPPVPRQASPQNLGSSGLLHPQMSPLLHSLVGQHILSVKQFTKDQMSHLFNVAHTLRMMVQKERSLDILKGKVMASMFYEVSTRTSSSFAAAMARLGGAVLSFSEATSSVQKGESLADSVQTMSCYADVIVLRHPQPGAVELAAKHCRRPVINAGDGVGEHPTQALLDIFTIREELGTVNGMTITMVGDLKHGRTVHSLACLLTQYRVSLRYVAPPSLRMPPSVRDFVASRGTKQEEFESIEEALPDTDVLYMTRIQKERFGSVQEYEACFGQFILTPHIMTRAKKKMVVMHPMPRVNEISVEVDSDPRAAYFRQAENGMYIRMALLATVLGRF

Target ORF information:

RefSeq Version	XM_006504091.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006504091.3

ORF Insert Sequence:

ATGGTCCCGC CCCTGACGTG CCCGGCCCCG CCCCTGACGT TGCCGGCTGC TTGCGCCGTC 
GCAGTCGTGC TTCAGCTCAC TACGCTTAGG GCTCTGGCTT GCCGCTCCCG CCTGCTCTCC 
AGCGCCCCGC GCAGCGAGCC ACGTGGACCA ACTCCGGCGC GCGGTGTTCG CTTGGTTCCA 
GTGGGGCTCG CCGCGCCTCC CGCGTCTGCG TGCTTGCCCT GTCTCAGCTC CGACCCGATG 
GCGGCCCTGG TGTTGGAGGA CGGGTCGGTG CTGCAGGGCC GGCCCTTTGG GGCCGCTGTG 
TCGACTGCTG GGGAAGTGGT GTTTCAGACC GGCATGGTCG GCTACCCAGA GGCCCTCACT 
GACCCTTCCT ACAAAGCTCA GATCCTAGTG CTAACGTATC CTCTCATCGG CAACTACGGC 
ATTCCCTCAG ATGAAGAGGA CGAGTTCGGC CTGAGCAAGT GGTTTGAATC CTCGGAGATC 
CACGTGGCGG GACTTGTAGT GGGAGAATGC TGTCCCACAC CCAGCCACTG GAGTGCCAAC 
TGCACCCTGC ATGAGTGGCT GCAACAGCGT GGCATCCCCG GCCTGCAAGG AGTGGACACT 
CGGGAGCTGA CTAAGAAGTT GCGGGAGCAA GGGTCTCTTT TGGGGAAGCT GGTCCAGAAA 
GGGACAGAGC CGTCAGCCCT GCCATTCGTG GACCCCAATG CCAGGCCCCT GGCACCAGAG 
GTCTCTATTA AGACTCCACG GGTATTCAAT GCAGGGGGTG CCCCTCGGAT CTGTGCTCTG 
GATTGTGGCC TCAAGTATAA TCAGATCCGA TGTCTCTGCC AGCTTGGGGC TGAAGTTACC 
GTGGTACCCT GGGACCACGA GTTAGACAGT CAGAAATATG ACGGTCTCTT CCTAAGTAAT 
GGACCCGGTG ACCCTGCCTC TTACCCGGGT GTGGTGTCCA CACTGAGCCG AGTCTTGTCT 
GAACCTAACC CCCGACCTGT GTTTGGAATC TGCCTTGGAC ACCAGCTGTT GGCTTTAGCC 
ATCGGGGCCA AAACTTACAA GATGAGATAC GGAAACCGAG GCCACAACCA GCCCTGTTTA 
CTGGTGGGCA CTGGGCGCTG CTTTCTGACG TCCCAGAATC ATGGGTTTGC AGTGGACGCA 
GATTCGCTGC CAGCAGGCTG GGCTCCTCTG TTCACCAATG CCAATGACTG TTCCAATGAA 
GGCATAGTGC ACGACAGCCT GCCCTTCTTC AGTGTCCAGT TTCACCCAGA GCACCGGGCC 
GGCCCTTCAG ATATGGAACT GCTCTTCGAT GTATTTCTAG AAACTGTGAG AGAGGCTGCA 
GCTGGGAACA TTGGGGGCCA GACAGTAAGA GAACGCTTGG CTCAGCGCCT CTGTCCCCCT 
GAGCTCCCCA TTCCTGGTTC TGGGCTCCCG CCTCCACGAA AGGTTCTAAT CCTAGGCTCA 
GGGGGCCTCT CCATTGGCCA AGCTGGAGAA TTTGACTACT CGGGCTCTCA GGCAATTAAA 
GCCCTAAAGG AGGAAAACAT CCAGACATTA CTAATCAACC CCAACATTGC CACAGTGCAG 
ACTTCTCAGG GGCTGGCAGA CAAGGTCTAT TTCCTTCCCA TAACACTTCA CTACGTAACC 
CAGGTAATTC GTAATGAACG CCCAGATGGT GTGTTACTGA CTTTTGGGGG TCAAACAGCC 
CTGAACTGCG GTGTGGAGCT GACCAAAGCT GGGGTACTGG CTCGATATGG GGTCCGGGTC 
TTGGGTACAC CCGTGGAGAC CATTGAACTG ACTGAGGACC GACGAGCCTT CGCTGCCAGG 
ATGGCTGAGA TCGGAGAGCA TGTCGCCCCC AGTGAAGCGG CAAATTCTCT TGAACAGGCT 
CAGGCAGCTG CCGAGCGACT AGGCTACCCT GTGCTGGTGC GAGCAGCCTT TGCCCTGGGT 
GGTCTCGGTT CTGGCTTTGC TTCCACCAAA GAGGAACTCT CAGCTCTTGT GGCTCCAGCT 
TTTGCCCATA CCAGTCAGGT GCTGATAGAC AAGTCTCTGA AGGGCTGGAA GGAGATTGAA 
TATGAGGTGG TGAGAGACGC CTATGGCAAC TGCGTAACGG TATGCAACAT GGAGAACTTA 
GACCCACTGG GCATTCATAC TGGTGAATCC ATAGTGGTGG CTCCGAGCCA GACACTGAAT 
GACAGAGAGT ACCAGCTTCT GCGACGGACA GCTATCAAGG TCACTCAGCA CCTGGGCATT 
GTCGGGGAGT GCAATGTACA GTATGCTTTG AACCCCGAGT CTGAGCAGTA TTACATCATT 
GAAGTGAATG CCAGGCTCTC CCGAAGCTCT GCCCTGGCCA GTAAGGCCAC AGGCTATCCA 
CTAGCCTATG TGGCAGCCAA GCTAGCATTG GGCATTCCCC TGCCAGAGCT CAGAAACTCT 
GTTACGGGAG GAACAGCAGC CTTTGAGCCC AGCCTGGACT ACTGCGTGGT AAAGATTCCT 
CGCTGGGACC TCAGCAAGTT CCTACGTGTC AGTACGAAGA TTGGGAGTTG CATGAAGAGT 
GTTGGCGAGG TCATGGGCAT CGGGCGGTCC TTTGAGGAGG CCTTCCAAAA GGCCCTGCGC 
ATGGTCGATG AGAACTGCGT AGGCTTCGAC CATACAGTAA AGCCAGTCAG CGACATGGAG 
CTGGAGACTC CGACGGATAA GCGGATCTTT GTGGTGGCTG CCGCTCTGTG GGCTGGCTAC 
TCTGTGGAGC GCCTCTACGA GCTCACCCGC ATTGATTGCT GGTTCCTGCA CCGGATGAAG 
CGCATTGTGA CCCACGCCCA GTTGCTGGAA CAACACCGTG GACAAGCTTT ACCCCAAGAC 
CTGCTGCACC AGGCCAAGTG CCTCGGCTTC TCAGACAAGC AGATTGCCCT GGCAGTCCTG 
AGCACAGAGC TGGCTGTTCG GAAGCTGCGT CAGGAACTGG GGATCTGCCC AGCGGTGAAA 
CAGATTGACA CAGTTGCAGC TGAGTGGCCA GCCCAGACAA ATTACCTGTA CCTGACATAC 
TGGGGCAACA CCCATGACCT CGACTTTCGG GCACCTCACG TCCTGGTCCT TGGCTCTGGT 
GTCTACCGGA TTGGCTCCAG TGTTGAGTTT GATTGGTGTG CTGTGGGTTG CATCCAGCAG 
CTCCGGAAGA TGGGTTATAA GACCATCATG GTGAACTACA ACCCAGAGAC AGTCAGCACC 
GACTATGATA TGTGTGACCG ACTCTACTTT GATGAGATCT CTTTTGAGGT GGTGATGGAC 
ATCTATGAGT TAGAGAACCC CGAAGGTGTG ATCCTGTCCA TGGGTGGGCA GCTGCCCAAC 
AACATGGCCA TGGCTTTGCA TCGGCAGCAG TGCCGAGTGC TGGGCACCTC CCCCGAAGCC 
ATCGACTCAG CCGAGAACCG ATTCAAGTTC TCCCGGCTCC TAGATACCAT CGGTATCAGC 
CAGCCTCAGT GGCGAGAGCT CAGTGACCTT GAGTCTGCTC GCCAGTTCTG CCACACGGTC 
GGGTACCCCT GCGTAGTGCG CCCCTCCTAT GTGCTCAGCG GTGCTGCTAT GAATGTGGCG 
TACACCGACG GGGACCTGGA GCGTTTCCTG AGCAGTGCGG CCGCCGTCTC TAAGGAGCAC 
CCCGTAGTCA TCTCCAAGTT CATTCAGGAA GCAAAGGAGA TCGACGTGGA CGCGGTGGCC 
TGTGACGGCA TCGTGTCGGC CATTGCCATC TCTGAGCACG TGGAGAATGC AGGTGTGCAT 
TCAGGTGATG CCACGCTGGT GACCCCCCCA CAAGACATCA CCCCGAAAAC TCTGGAGCGG 
ATCAAAGCCA TTGTGCATGC TGTGGGCCAG GAGCTACAGG TTACAGGACC CTTCAATCTG 
CAGCTCATTG CCAAGGATGA CCAGCTGAAG GTTATTGAAT GCAACGTGCG TGTATCTCGC 
TCTTTCCCCT TCGTGTCCAA GACACTGGGT GTTGATCTAG TAGCCTTGGC CACAAGGATC 
ATCATGGGGG AGAAAGTAGA GCCCGTGGGG CTCATGACTG GCTCTGGAGT CGTGGGAGTA 
AAGGTACCTC AGTTCTCCTT CTCACGCTTG GCCGGTGCCG ACGTGGTACT TGGTGTGGAG 
ATGACCAGTA CTGGAGAGGT GGCCGGCTTC GGAGAGAGCC GTTGTGAAGC CTACCTCAAA 
GCCATGCTCA GCACTGGCTT TAAGATCCCA GAGAAGAACA TCCTGCTGAC TATTGGCAGC 
TACAAGAACA AAAGTGAGCT GCTCCCAACT GTGCGGTTGC TGGAGAGCCT GGGCTACAGC 
CTCTATGCCA GCCTGGGTAC GGCAGACTTC TACACTGAGC ACGGGGTCAA GGTAACAGCT 
GTGGACTGGC ACTTTGAAGA GGCGGTGGAT GGTGAGTGCC CACCACAACG GAGCATCTTG 
GATCAGCTGG CTGAAAATCA CTTTGAGCTA GTGATTAACC TGTCCATGCG TGGGGCTGGG 
GGTCGGCGTC TTTCCTCCTT CGTTACCAAG GGCTACCGTA CCCGGCGTCT GGCCGCTGAT 
TTCTCTGTGC CTCTCATCAT TGACATCAAG TGCACCAAAC TCTTTGTGGA GGCCCTAGGC 
CAGATCGGCC CAGCCCCTCC TTTGAAAGTG CACGTTGACT GCATGACCTC CCAGAAGCTA 
GTGCGCCTGC CCGGATTGAT TGATGTCCAC GTGCACCTTC GGGAACCAGG TGGGACACAC 
AAAGAGGACT TTGCCTCAGG CACAGCTGCT GCCTTGGCTG GGGGTGTCAC CATGGTTTGT 
GCTATGCCTA ATACCCGACC CCCCATCATT GATGCTCCTG CTCTCGCCCT TGCCCAGAAG 
CTGGCAGAGG CTGGCGCCCG CTGTGACTTT ACCTTATTCC TTGGAGCCTC ATCTGAGAAC 
GCGGGGACTC TGGGTGCTGT GGCCGGCTCT GCAGCAGGCC TGAAGCTCTA CCTCAACGAG 
ACCTTCTCTG AGCTGCGGCT GGACAGTGTG GCCCAGTGGA TGGAGCATTT TGAAACCTGG 
CCTGCCCACC TCCCCATTGT GGCCCATGCA GAGCGGCAGA GTGTCGCTGC GGTCCTCATG 
GTGGCTCAGC TGACCCAGCG TCCAGTGCAC ATATGTCATG TGGCTCGGAA GGAAGAGATC 
CTGTTGATTA AAACTGCAAA GGCGCAGGGG CTGCCAGTGA CCTGTGAGGT CGCTCCTCAC 
CACCTGTTCC TAAACCGGGA AGACTTGGAG CGCCTGGGAC CTGGGAAGGG AGAGGTCCGT 
CCTGAGCTTG GGTCCCGAGA GGATATGGAA GCCCTGTGGG AGAATATGGC AGTCATCGAC 
TGTTTTGCCT CAGACCACGC TCCCCATACC TTGGAAGAGA AGTGTGGGCC CAAGCCTCCA 
CCTGGCTTCC CGGGCCTAGA GACCATGTTG CCGCTGCTGC TGACAGCTGT GAGCGAGGGC 
CGCCTCAGTC TGGATGACCT GCTGCAGCGA TTGCACCACA ACCCTCGGCG GATCTTCCAC 
CTGCCTCTCC AGGAGGACAC CTATGTAGAG GTGGATCTGG AGCATGAGTG GACAGTCCCC 
AGCCACATGC CCTTCTCTAA GGCCCGCTGG ACCCCATTTG AAGGACAGAA GGTGAAGGGC 
ACAGTGCGCC GTGTGGTCTT GCGAGGGGAG GTCGCCTATA TTGATGGACA GGTGCTGGTA 
CCCCCGGGCT ATGGACAAGA TGTACGGAAG TGGCCGCAGG GGGTTGTTCC CCAGCCCCCT 
CCTTCAACTC CCGCCACCAC CGAAATCACC ACGACACCTG AGCGACCACG CCGAGTCATC 
CCGGGCCTTC CTGATGGCCG CTTCCACCTG CCACCCCGCA TCCACCGGGC CTCTGACCCA 
GGTCTGCCAG CTCTGTACCT CCGCCCAGGA GCTGGGATCT CATGGGGCAG CAGAGCATGG 
GCTGAGGAAC CTAAAGAGAA GCCACCCAGG AAGGTAGTGG AACCAGAGCT GATGGGAACC 
CCTGATGGTC CCTGCTACCC TGCACCACCA GTACCTAGAC AGGCATCACC CCAGAACCTA 
GGGTCTTCTG GCCTGCTGCA CCCCCAGATG TCGCCCCTAC TGCACTCCCT AGTGGGCCAG 
CACATCCTGT CTGTCAAGCA GTTCACTAAG GATCAGATGT CTCATCTGTT CAATGTGGCG 
CACACGCTAC GGATGATGGT ACAGAAGGAG CGGAGCCTCG ACATCCTGAA GGGGAAGGTC 
ATGGCCTCCA TGTTCTACGA GGTGAGCACC CGCACCAGTA GCTCCTTCGC AGCAGCCATG 
GCCCGGCTCG GGGGCGCTGT TCTCAGCTTC TCAGAAGCTA CATCCTCCGT CCAAAAGGGC 
GAGTCCCTTG CTGACTCGGT GCAGACCATG AGTTGCTATG CTGATGTCAT TGTGCTCCGA 
CACCCTCAGC CTGGAGCCGT GGAGCTGGCA GCCAAGCACT GTCGCAGGCC AGTGATCAAT 
GCTGGGGATG GAGTTGGGGA GCACCCCACA CAGGCCCTGC TGGACATCTT CACCATTCGA 
GAAGAGCTGG GGACTGTCAA TGGCATGACG ATCACTATGG TCGGTGACCT GAAGCATGGA 
CGCACAGTGC ACTCCCTGGC CTGCCTGCTC ACCCAGTACC GTGTGAGCCT ACGCTACGTG 
GCACCTCCCA GCCTGCGCAT GCCACCCAGT GTACGGGACT TTGTGGCCTC CCGAGGCACC 
AAGCAGGAGG AGTTTGAGAG CATCGAGGAG GCGCTGCCCG ACACAGACGT GCTCTACATG 
ACTCGGATCC AGAAAGAACG ATTTGGCTCT GTCCAGGAAT ATGAAGCTTG CTTTGGTCAG 
TTCATCCTCA CTCCTCACAT CATGACCCGG GCCAAGAAGA AGATGGTGGT GATGCACCCA 
ATGCCCCGTG TCAATGAGAT AAGTGTGGAG GTGGACTCGG ACCCCCGGGC AGCCTACTTC 
CGCCAAGCTG AGAACGGCAT GTACATCCGC ATGGCCCTGC TTGCCACCGT GCTTGGCCGT 
TTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Novel role for caspase-activated DNase in the regulation of pathological cardiac hypertrophy.
Hypertension (Dallas, Tex. : 1979)65(4)871-81(2015 Apr)
Gao L,Huang K,Jiang DS,Liu X,Huang D,Li H,Zhang XD,Huang K

Quantitative trait loci affecting the 3D skull shape and size in mouse and prioritization of candidate genes in-silico.
Frontiers in physiology692(2015)
Maga AM,Navarro N,Cunningham ML,Cox TC

Miller (Genee-Wiedemann) syndrome represents a clinically and biochemically distinct subgroup of postaxial acrofacial dysostosis associated with partial deficiency of DHODH.
Human molecular genetics21(18)3969-83(2012 Sep)
Rainger J,Bengani H,Campbell L,Anderson E,Sokhi K,Lam W,Riess A,Ansari M,Smithson S,Lees M,Mercer C,McKenzie K,Lengfeld T,Gener Querol B,Branney P,McKay S,Morrison H,Medina B,Robertson M,Kohlhase J,Gordon C,Kirk J,Wieczorek D,Fitzpatrick DR

Autophagy suppresses tumor progression by limiting chromosomal instability.
Genes & development21(11)1367-81(2007 Jun)
Mathew R,Kongara S,Beaudoin B,Karp CM,Bray K,Degenhardt K,Chen G,Jin S,White E

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Cad cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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