Ucma cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ucma
Entrez Gene ID	68527
Full Name	upper zone of growth plate and cartilage matrix associated
Synonyms	1110017I16Rik,AW121955,Grp
General protein information	Preferred Names upper zone of growth plate and cartilage matrix associated Names unique cartilage matrix-associated protein Gla-rich protein upper zone of growth plate and cartilage matrix associated protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 A1
Summary	This gene encodes chondrocyte-specific, highly charged proteins that are abundantly expressed during the early stages of chondrogenesis. The encoded protein undergoes proteolytic processing to generate a mature protein that is secreted into the extracellular matrix. The glutamic acid residues in the encoded protein undergo gamma carboxylation in a vitamin K-dependent manner. Despite the implied role in calcification and ossification, mice lacking the encoded protein do not display significant defects in the skeletal development. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo a similar proteolytic processing to generate mature proteins. [provided by RefSeq, Aug 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001113558.2	NP_001107030.1	unique cartilage matrix-associated protein isoform b preproprotein
NM_001165932.1	NP_001159404.1	unique cartilage matrix-associated protein isoform c precursor
NM_026754.3	NP_081030.1	unique cartilage matrix-associated protein isoform a precursor
NM_001311208.1	NP_001298137.1	unique cartilage matrix-associated protein isoform d precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	UCMA	XP_535186.2
Danio rerio (zebrafish)	ucmab	NP_998099.1
Homo sapiens (human)	UCMA	NP_660357.2
	LOC698795	XP_001087282.2
Mus musculus (house mouse)	Ucma	NP_001107030.1
Bos taurus (cattle)	UCMA	NP_001193155.1
Xenopus tropicalis (tropical clawed frog)	ucma	NP_001016485.1
Pan troglodytes (chimpanzee)	UCMA	XP_001140953.1
Rattus norvegicus (Norway rat)	Ucma	NP_001099591.1

Gene Ontology
Bibliography

Related articles in PubMed

Insights into the association of Gla-rich protein and osteoarthritis, novel splice variants and ?-carboxylation status.
Rafael MS, Cavaco S, Viegas CS, Santos S, Ramos A, Willems BA, Herfs M, Theuwissen E, Vermeer C, Simes DC
Molecular nutrition & food research58(8)1636-46(2014 Aug)

Identification of four alternatively spliced transcripts of the Ucma/GRP gene, encoding a new Gla-containing protein.
Le Jeune M, Tomavo N, Tian TV, Flourens A, Marchand N, Camuzeaux B, Mallein-Gerin F, Duterque-Coquillaud M
Experimental cell research316(2)203-15(2010 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that a novel splice variant of GRP is expressed in cartilage and other tissues (i.e. GRP-F7); GRP-F1 is predominant splice variant expressed in adult tissues.
Title: Insights into the association of Gla-rich protein and osteoarthritis, novel splice variants and ?-carboxylation status.

Considering that skeletal development in Ucma-deficient mice is not significantly impaired, protein expression in adult cartilage indicates that Ucma might be involved in skeletal homeostasis and in the mechanical properties of the skeleton
Title: Ucma is not necessary for normal development of the mouse skeleton.

Four alternative splicing variants derived from the mouse Ucma/GRP gene in chondrocytes were identified and their expression and subcellular localization during chondrogenesis was determined.
Title: Identification of four alternatively spliced transcripts of the Ucma/GRP gene, encoding a new Gla-containing protein.

Ucma may be involved in the negative control of osteogenic differentiation of osteochondrogenic precursor cells
Title: Ucma, a novel secreted cartilage-specific protein with implications in osteogenesis.

The present data strongly suggest an important function of Ucma in the early phase of chondrocyte differentiation.
Title: Ucma--A novel secreted factor represents a highly specific marker for distal chondrocytes.

The following Ucma gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ucma cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu05154	NM_001113558.2 Latest version!	Mus musculus upper zone of growth plate and cartilage matrix associated (Ucma), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu02948	NM_026754.3 Latest version!	Mus musculus upper zone of growth plate and cartilage matrix associated (Ucma), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu10137	NM_001165932.1 Latest version!	Mus musculus upper zone of growth plate and cartilage matrix associated (Ucma), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu70276	NM_001311208.1 Latest version!	Mus musculus upper zone of growth plate and cartilage matrix associated (Ucma), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ucma ( NM_001113558.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001107030.1 Mus musculus upper zone of growth plate and cartilage matrix associated (Ucma), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu05154
Clone ID Related Accession (Same CDS sequence)	NM_001113558.2
Accession Version	NM_001113558.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-31
Organism	Mus musculus(house mouse)
Product	unique cartilage matrix-associated protein isoform b preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EU368184.1 and BY590633.1. On Sep 30, 2009 this sequence version replaced NM_001113558.1. Transcript Variant: This variant (2, also known as Ucma/GRP-F1) contains an alternate in-frame exon in the 5' coding region compared to variant 1. The encoded isoform (b) is longer than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU368184.1, CB588171.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849383, SAMN00849390 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCTGGA GACGGGTCAT TCTCCTGTCA TCTCTCTTGG CCCTGGTGCT CCTGTGTATG 
CTACAGGAGG GGACCAGCGC TTCTGTGGGG AGCAGGCAGG CAGCTGCAGA GGGGGTGCAG 
GAAGGTGTGA AACAGAAGAT TTTCATGCAA GAATCTGATG CCTCCAATTT CCTCAAGAGG 
CGTGGCAAGC GGTCTCCTAA GTCCCGAGAT GAAGTTAATG CGGAAAACAG ACAGAGGCTG 
CGGGATGATG AGCTGCGGAG GGAGTATTAC GAGGAGCAAA GGAACGAGTT TGAGAACTTC 
GTGGAGGAAC AGAGAGATGA GCAGGAAGAG AGGACCCGGG AGGCTGTGGA GCAGTGGCGC 
CAGTGGCATT ATGATGGCCT GTATCCTTCC TACCTCTACA ACCGCCAAAA CATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001107030.1
CDS	131..547
Translation	MSWRRVILLSSLLALVLLCMLQEGTSASVGSRQAAAEGVQEGVKQKIFMQESDASNFLKRRGKRSPKSRDEVNAENRQRLRDDELRREYYEEQRNEFENFVEEQRDEQEERTREAVEQWRQWHYDGLYPSYLYNRQNI

Target ORF information:

RefSeq Version	NM_001113558.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus upper zone of growth plate and cartilage matrix associated (Ucma), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001113558.2

ORF Insert Sequence:

ATGTCCTGGA GACGGGTCAT TCTCCTGTCA TCTCTCTTGG CCCTGGTGCT CCTGTGTATG 
CTACAGGAGG GGACCAGCGC TTCTGTGGGG AGCAGGCAGG CAGCTGCAGA GGGGGTGCAG 
GAAGGTGTGA AACAGAAGAT TTTCATGCAA GAATCTGATG CCTCCAATTT CCTCAAGAGG 
CGTGGCAAGC GGTCTCCTAA GTCCCGAGAT GAAGTTAATG CGGAAAACAG ACAGAGGCTG 
CGGGATGATG AGCTGCGGAG GGAGTATTAC GAGGAGCAAA GGAACGAGTT TGAGAACTTC 
GTGGAGGAAC AGAGAGATGA GCAGGAAGAG AGGACCCGGG AGGCTGTGGA GCAGTGGCGC 
CAGTGGCATT ATGATGGCCT GTATCCTTCC TACCTCTACA ACCGCCAAAA CATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ucma ( NM_026754.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_081030.1 Mus musculus upper zone of growth plate and cartilage matrix associated (Ucma), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu02948
Clone ID Related Accession (Same CDS sequence)	NM_026754.3
Accession Version	NM_026754.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-31
Organism	Mus musculus(house mouse)
Product	unique cartilage matrix-associated protein isoform a precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EU368184.1, AK003750.1 and BY590633.1. On Sep 30, 2009 this sequence version replaced NM_026754.2. Transcript Variant: This variant (1, also known as Ucma/GRP-F2) encodes isoform a. This isoform (a) may undergo proteolytic processing similar to isoform b. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BY704011.1, AK003750.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164132, SAMN01164136 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCTGGA GACGGGTCAT TCTCCTGTCA TCTCTCTTGG CCCTGGTGCT CCTGTGTAGT 
GTGAAACAGA AGATTTTCAT GCAAGAATCT GATGCCTCCA ATTTCCTCAA GAGGCGTGGC 
AAGCGGTCTC CTAAGTCCCG AGATGAAGTT AATGCGGAAA ACAGACAGAG GCTGCGGGAT 
GATGAGCTGC GGAGGGAGTA TTACGAGGAG CAAAGGAACG AGTTTGAGAA CTTCGTGGAG 
GAACAGAGAG ATGAGCAGGA AGAGAGGACC CGGGAGGCTG TGGAGCAGTG GCGCCAGTGG 
CATTATGATG GCCTGTATCC TTCCTACCTC TACAACCGCC AAAACATCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_081030.1
CDS	131..481
Translation	MSWRRVILLSSLLALVLLCSVKQKIFMQESDASNFLKRRGKRSPKSRDEVNAENRQRLRDDELRREYYEEQRNEFENFVEEQRDEQEERTREAVEQWRQWHYDGLYPSYLYNRQNI

Target ORF information:

RefSeq Version	NM_026754.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus upper zone of growth plate and cartilage matrix associated (Ucma), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_026754.3

ORF Insert Sequence:

ATGTCCTGGA GACGGGTCAT TCTCCTGTCA TCTCTCTTGG CCCTGGTGCT CCTGTGTAGT 
GTGAAACAGA AGATTTTCAT GCAAGAATCT GATGCCTCCA ATTTCCTCAA GAGGCGTGGC 
AAGCGGTCTC CTAAGTCCCG AGATGAAGTT AATGCGGAAA ACAGACAGAG GCTGCGGGAT 
GATGAGCTGC GGAGGGAGTA TTACGAGGAG CAAAGGAACG AGTTTGAGAA CTTCGTGGAG 
GAACAGAGAG ATGAGCAGGA AGAGAGGACC CGGGAGGCTG TGGAGCAGTG GCGCCAGTGG 
CATTATGATG GCCTGTATCC TTCCTACCTC TACAACCGCC AAAACATCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ucma ( NM_001165932.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001159404.1 Mus musculus upper zone of growth plate and cartilage matrix associated (Ucma), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu10137
Clone ID Related Accession (Same CDS sequence)	NM_001165932.1
Accession Version	NM_001165932.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 318bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-31
Organism	Mus musculus(house mouse)
Product	unique cartilage matrix-associated protein isoform c precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EU368184.1, BE648935.1 and BY590633.1. Transcript Variant: This variant (3, also known as Ucma/GRP-F3) contains an alternate in-frame exon in the 5' coding region and lacks an in-frame exon in the 3' coding region, compared to variant 1. The encoded isoform (c) is shorter than isoform a. This isoform (c) may undergo proteolytic processing similar to isoform b. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: FJ217397.1, BY340411.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164136 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCTGGA GACGGGTCAT TCTCCTGTCA TCTCTCTTGG CCCTGGTGCT CCTGTGTATG 
CTACAGGAGG GGACCAGCGC TTCTGTGGGG AGCAGGCAGG CAGCTGCAGA GGGGGTGCAG 
GAAGGTGTGA AACAGAAGAT TTTCATGCAA GAATCTGATG CCTCCAATTT CCTCAAGAGG 
CGTGGCAAGC GGTCTCCTAA GTCCCGAGAT GAAGTTAATG AGCAGGAAGA GAGGACCCGG 
GAGGCTGTGG AGCAGTGGCG CCAGTGGCAT TATGATGGCC TGTATCCTTC CTACCTCTAC 
AACCGCCAAA ACATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001159404.1
CDS	131..448
Translation	MSWRRVILLSSLLALVLLCMLQEGTSASVGSRQAAAEGVQEGVKQKIFMQESDASNFLKRRGKRSPKSRDEVNEQEERTREAVEQWRQWHYDGLYPSYLYNRQNI

Target ORF information:

RefSeq Version	NM_001165932.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus upper zone of growth plate and cartilage matrix associated (Ucma), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001165932.1

ORF Insert Sequence:

ATGTCCTGGA GACGGGTCAT TCTCCTGTCA TCTCTCTTGG CCCTGGTGCT CCTGTGTATG 
CTACAGGAGG GGACCAGCGC TTCTGTGGGG AGCAGGCAGG CAGCTGCAGA GGGGGTGCAG 
GAAGGTGTGA AACAGAAGAT TTTCATGCAA GAATCTGATG CCTCCAATTT CCTCAAGAGG 
CGTGGCAAGC GGTCTCCTAA GTCCCGAGAT GAAGTTAATG AGCAGGAAGA GAGGACCCGG 
GAGGCTGTGG AGCAGTGGCG CCAGTGGCAT TATGATGGCC TGTATCCTTC CTACCTCTAC 
AACCGCCAAA ACATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ucma ( NM_001311208.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001298137.1 Mus musculus upper zone of growth plate and cartilage matrix associated (Ucma), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OMu70276
Clone ID Related Accession (Same CDS sequence)	NM_001311208.1
Accession Version	NM_001311208.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 252bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Mus musculus(house mouse)
Product	unique cartilage matrix-associated protein isoform d precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EU368184.1, FJ217398.1 and BY590633.1. Transcript Variant: This variant (4, also known as Ucma/GRP-F4) lacks an alternate in-frame exon in the 3' coding region, compared to variant 1. The encoded isoform (d) is shorter than isoform a. This isoform (d) may undergo proteolytic processing similar to isoform b. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: FJ217398.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164136, SAMN01164138 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCTGGA GACGGGTCAT TCTCCTGTCA TCTCTCTTGG CCCTGGTGCT CCTGTGTAGT 
GTGAAACAGA AGATTTTCAT GCAAGAATCT GATGCCTCCA ATTTCCTCAA GAGGCGTGGC 
AAGCGGTCTC CTAAGTCCCG AGATGAAGTT AATGAGCAGG AAGAGAGGAC CCGGGAGGCT 
GTGGAGCAGT GGCGCCAGTG GCATTATGAT GGCCTGTATC CTTCCTACCT CTACAACCGC 
CAAAACATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001298137.1
CDS	131..382
Translation	MSWRRVILLSSLLALVLLCSVKQKIFMQESDASNFLKRRGKRSPKSRDEVNEQEERTREAVEQWRQWHYDGLYPSYLYNRQNI

Target ORF information:

RefSeq Version	NM_001311208.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus upper zone of growth plate and cartilage matrix associated (Ucma), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001311208.1

ORF Insert Sequence:

ATGTCCTGGA GACGGGTCAT TCTCCTGTCA TCTCTCTTGG CCCTGGTGCT CCTGTGTAGT 
GTGAAACAGA AGATTTTCAT GCAAGAATCT GATGCCTCCA ATTTCCTCAA GAGGCGTGGC 
AAGCGGTCTC CTAAGTCCCG AGATGAAGTT AATGAGCAGG AAGAGAGGAC CCGGGAGGCT 
GTGGAGCAGT GGCGCCAGTG GCATTATGAT GGCCTGTATC CTTCCTACCT CTACAACCGC 
CAAAACATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Insights into the association of Gla-rich protein and osteoarthritis, novel splice variants and ?-carboxylation status.
Molecular nutrition & food research58(8)1636-46(2014 Aug)
Rafael MS,Cavaco S,Viegas CS,Santos S,Ramos A,Willems BA,Herfs M,Theuwissen E,Vermeer C,Simes DC

Identification of four alternatively spliced transcripts of the Ucma/GRP gene, encoding a new Gla-containing protein.
Experimental cell research316(2)203-15(2010 Jan)
Le Jeune M,Tomavo N,Tian TV,Flourens A,Marchand N,Camuzeaux B,Mallein-Gerin F,Duterque-Coquillaud M

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Ucma cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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