Phf5a cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Phf5a
Entrez Gene ID	68479
Full Name	PHD finger protein 5A
Synonyms	1110007B08Rik
General protein information	Preferred Names PHD finger protein 5A Names PHD finger-like domain-containing protein 5A PHD finger-like domain protein 5A SF3b14b splicing factor 3B-associated 14 kDa protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 15 Map Location: 15\|15 E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_026737.3	NP_081013.1	PHD finger-like domain-containing protein 5A

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	RDS3	NP_015419.1
Arabidopsis thaliana (thale cress)	AT1G07170	NP_001077473.1
Oryza sativa (rice)	Os05g0367000	NP_001055334.1
Xenopus tropicalis (tropical clawed frog)	phf5a	NP_001123712.1
Pan troglodytes (chimpanzee)	PHF5A	XP_003317310.1
Drosophila melanogaster (fruit fly)	CG9548	NP_609038.1
Bos taurus (cattle)	PHF5A	NP_001076851.1
Gallus gallus (chicken)	PHF5A	NP_001264305.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009905	XP_319026.1
Caenorhabditis elegans (roundworm)	phf-5	NP_001022909.1
Kluyveromyces lactis	KLLA0C15675g	XP_452900.1
Magnaporthe oryzae (rice blast fungus)	MGG_05173	XP_003712711.1
Arabidopsis thaliana (thale cress)	AT2G30000	NP_565691.1
Oryza sativa (rice)	Os04g0663300	NP_001054163.1
Mus musculus (house mouse)	Phf5a	NP_081013.1
Danio rerio (zebrafish)	phf5a	NP_775373.1
Schizosaccharomyces pombe (fission yeast)	ini1	NP_593792.1
Neurospora crassa	NCU00348	XP_957819.2
Homo sapiens (human)	PHF5A	NP_116147.1
	LOC711683	XP_002798444.1
Canis lupus familiaris (dog)	PHF5A	XP_531718.1
Rattus norvegicus (Norway rat)	Phf5a	NP_620243.1
Eremothecium gossypii	AGOS_ABR103C	NP_983050.1

Gene Ontology
Bibliography

Related articles in PubMed

Regulation of transcriptional elongation in pluripotency and cell differentiation by the PHD-finger protein Phf5a.
Strikoudis A, Lazaris C, Trimarchi T, Galvao Neto AL, Yang Y, Ntziachristos P, Rothbart S, Buckley S, Dolgalev I, Stadtfeld M, Strahl BD, Dynlacht BD, Tsirigos A, Aifantis I
Nature cell biology18(11)1127-1138(2016 Nov)

The AERO system: a 3D-like approach for recording gene expression patterns in the whole mouse embryo.
Shimizu H, Kubo A, Uchibe K, Hashimoto M, Yokoyama S, Takada S, Mitsuoka K, Asahara H
PloS one8(10)e75754(2013)

A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58.
Yokoyama S, Ito Y, Ueno-Kudoh H, Shimizu H, Uchibe K, Albini S, Mitsuoka K, Miyaki S, Kiso M, Nagai A, Hikata T, Osada T, Fukuda N, Yamashita S, Harada D, Mezzano V, Kasai M, Puri PL, Hayashizaki Y, Okado H, Hashimoto M, Asahara H
Developmental cell17(6)836-48(2009 Dec)

PHF5A represents a bridge protein between splicing proteins and ATP-dependent helicases and is differentially expressed during mouse spermatogenesis.
Rzymski T, Grzmil P, Meinhardt A, Wolf S, Burfeind P
Cytogenetic and genome research121(3-4)232-44(2008)

Identification and characterization of a novel murine multigene family containing a PHD-finger-like motif.
Trappe R, Ahmed M, Gl?ser B, Vogel C, Tascou S, Burfeind P, Engel W
Biochemical and biophysical research communications293(2)816-26(2002 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Phf5a controls differentiation of adult myoblasts
Title: Regulation of transcriptional elongation in pluripotency and cell differentiation by the PHD-finger protein Phf5a.

results indicate that PHF5A resembles a protein which interacts with splicing factors U2AF1 and SFRS5 and helicases EP400 and DDX1 and functions as a bridge protein between these proteins
Title: PHF5A represents a bridge protein between splicing proteins and ATP-dependent helicases and is differentially expressed during mouse spermatogenesis.

molecular cloning and characterization of Phf5a
Title: Identification and characterization of a novel murine multigene family containing a PHD-finger-like motif.

The following Phf5a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Phf5a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu14769	NM_026737.3 Latest version!	Mus musculus PHD finger protein 5A (Phf5a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Phf5a ( NM_026737.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_081013.1 Mus musculus PHD finger protein 5A (Phf5a), mRNA.

Price & Availability↑

CloneID	OMu14769
Clone ID Related Accession (Same CDS sequence)	NM_026737.3
Accession Version	NM_026737.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Mus musculus(house mouse)
Product	PHD finger-like domain-containing protein 5A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AK143590.1. On Apr 5, 2007 this sequence version replaced NM_026737.2. ##Evidence-Data-START## Transcript exon combination :: AK143590.1, BC025161.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164135, SAMN01164137 [ECO:0000348] ##Evidence-Data-END##

ATGGCTAAAC ATCATCCAGA TTTGATTTTC TGCCGCAAGC AGGCTGGTGT GGCTATCGGA 
AGACTGTGTG AAAAATGTGA CGGCAAGTGT GTGATCTGTG ATTCCTACGT GCGTCCCTGC 
ACCCTGGTCC GCATATGTGA TGAGTGTAAC TATGGATCTT ACCAGGGCCG GTGTGTAATC 
TGTGGCGGCC CCGGAGTCTC CGATGCCTAC TACTGTAAAG AGTGCACCAT TCAGGAGAAG 
GATAGAGATG GTTGTCCAAA GATTGTCAAT TTGGGGAGCT CTAAGACAGA CCTGTTCTAT 
GAACGCAAAA AATACGGCTT CAAGAAGAGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_081013.1
CDS	29..361
Translation	MAKHHPDLIFCRKQAGVAIGRLCEKCDGKCVICDSYVRPCTLVRICDECNYGSYQGRCVICGGPGVSDAYYCKECTIQEKDRDGCPKIVNLGSSKTDLFYERKKYGFKKR

Target ORF information:

RefSeq Version	NM_026737.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus PHD finger protein 5A (Phf5a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_026737.3

ORF Insert Sequence:

ATGGCTAAAC ATCATCCAGA TTTGATTTTC TGCCGCAAGC AGGCTGGTGT GGCTATCGGA 
AGACTGTGTG AAAAATGTGA CGGCAAGTGT GTGATCTGTG ATTCCTACGT GCGTCCCTGC 
ACCCTGGTCC GCATATGTGA TGAGTGTAAC TATGGATCTT ACCAGGGCCG GTGTGTAATC 
TGTGGCGGCC CCGGAGTCTC CGATGCCTAC TACTGTAAAG AGTGCACCAT TCAGGAGAAG 
GATAGAGATG GTTGTCCAAA GATTGTCAAT TTGGGGAGCT CTAAGACAGA CCTGTTCTAT 
GAACGCAAAA AATACGGCTT CAAGAAGAGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Regulation of transcriptional elongation in pluripotency and cell differentiation by the PHD-finger protein Phf5a.
Nature cell biology18(11)1127-1138(2016 Nov)
Strikoudis A,Lazaris C,Trimarchi T,Galvao Neto AL,Yang Y,Ntziachristos P,Rothbart S,Buckley S,Dolgalev I,Stadtfeld M,Strahl BD,Dynlacht BD,Tsirigos A,Aifantis I

The AERO system: a 3D-like approach for recording gene expression patterns in the whole mouse embryo.
PloS one8(10)e75754(2013)
Shimizu H,Kubo A,Uchibe K,Hashimoto M,Yokoyama S,Takada S,Mitsuoka K,Asahara H

A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58.
Developmental cell17(6)836-48(2009 Dec)
Yokoyama S,Ito Y,Ueno-Kudoh H,Shimizu H,Uchibe K,Albini S,Mitsuoka K,Miyaki S,Kiso M,Nagai A,Hikata T,Osada T,Fukuda N,Yamashita S,Harada D,Mezzano V,Kasai M,Puri PL,Hayashizaki Y,Okado H,Hashimoto M,Asahara H

PHF5A represents a bridge protein between splicing proteins and ATP-dependent helicases and is differentially expressed during mouse spermatogenesis.
Cytogenetic and genome research121(3-4)232-44(2008)
Rzymski T,Grzmil P,Meinhardt A,Wolf S,Burfeind P

Identification and characterization of a novel murine multigene family containing a PHD-finger-like motif.
Biochemical and biophysical research communications293(2)816-26(2002 May)
Trappe R,Ahmed M,Gl?ser B,Vogel C,Tascou S,Burfeind P,Engel W

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Phf5a cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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