Nudt21 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nudt21
Entrez Gene ID	68219
Full Name	nudix (nucleoside diphosphate linked moiety X)-type motif 21
Synonyms	25kDa,3110048P04Rik,5730530J16Rik,AU014860,AW549947,Cpsf5
General protein information	Preferred Names nudix (nucleoside diphosphate linked moiety X)-type motif 21 Names cleavage and polyadenylation specificity factor subunit 5 cleavage and polyadenylation specific factor 5, 25 kD subunit nucleoside diphosphate-linked moiety X motif 21 nudix motif 21
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8\|8 C5

mRNA and Protein(s)

mRNA	Protein	Name
NM_026623.3	NP_080899.1	cleavage and polyadenylation specificity factor subunit 5

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	NUDT21	NP_001264603.1
Drosophila melanogaster (fruit fly)	CG3689	NP_001036597.1
Neurospora crassa	NCU09014	XP_958148.1
Arabidopsis thaliana (thale cress)	AT4G25550	NP_194285.2
Rattus norvegicus (Norway rat)	Nudt21	NP_001034093.1
Danio rerio (zebrafish)	cpsf5	NP_957411.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007242	XP_308558.3
Pan troglodytes (chimpanzee)	NUDT21	XP_003952896.1
	NUDT21	XP_001096261.1
Mus musculus (house mouse)	Nudt21	NP_080899.1
Caenorhabditis elegans (roundworm)	cfim-1	NP_492334.1
Magnaporthe oryzae (rice blast fungus)	MGG_01676	XP_003714664.1
Oryza sativa (rice)	Os04g0683100	NP_001054301.1
Homo sapiens (human)	NUDT21	NP_008937.1
Canis lupus familiaris (dog)	NUDT21	XP_535298.2
Bos taurus (cattle)	NUDT21	NP_001030408.1
Xenopus tropicalis (tropical clawed frog)	nudt21	NP_001017262.1

Gene Ontology
Bibliography

Related articles in PubMed

Nudt21 Controls Cell Fate by Connecting Alternative Polyadenylation to Chromatin Signaling.
Brumbaugh J, Di Stefano B, Wang X, Borkent M, Forouzmand E, Clowers KJ, Ji F, Schwarz BA, Kalocsay M, Elledge SJ, Chen Y, Sadreyev RI, Gygi SP, Hu G, Shi Y, Hochedlinger K
Cell172(1-2)106-120.e21(2018 Jan)

Pre-messenger RNA cleavage factor I (CFIm): potential role in alternative polyadenylation during spermatogenesis.
Sartini BL, Wang H, Wang W, Millette CF, Kilpatrick DL
Biology of reproduction78(3)472-82(2008 Mar)

A genome-wide in situ hybridization map of RNA-binding proteins reveals anatomically restricted expression in the developing mouse brain.
McKee AE, Minet E, Stern C, Riahi S, Stiles CD, Silver PA
BMC developmental biology514(2005 Jul)

Interaction of poly(A) polymerase with the 25-kDa subunit of cleavage factor I.
Kim H, Lee Y
Biochemical and biophysical research communications289(2)513-8(2001 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Here we report that the CFIm subunits NUDT21/CPSF5 and CPSF6 are highly enriched in mouse male germ cells relative to somatic cells.
Title: Pre-messenger RNA cleavage factor I (CFIm): potential role in alternative polyadenylation during spermatogenesis.

The following Nudt21 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nudt21 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu14927	NM_026623.3 Latest version!	Mus musculus nudix (nucleoside diphosphate linked moiety X)-type motif 21 (Nudt21), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nudt21 ( NM_026623.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_080899.1 Mus musculus nudix (nucleoside diphosphate linked moiety X)-type motif 21 (Nudt21), mRNA.

Price & Availability↑

CloneID	OMu14927
Clone ID Related Accession (Same CDS sequence)	NM_026623.3
Accession Version	NM_026623.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 684bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Mus musculus(house mouse)
Product	cleavage and polyadenylation specificity factor subunit 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC138118.4 and AK011688.2. On Jul 1, 2006 this sequence version replaced NM_026623.2. ##Evidence-Data-START## Transcript exon combination :: SRR1660821.129270.1, AK019433.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164133, SAMN01164143 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTGTGG TGCCGCCCAA TCGCTCGCAG ACGGGCTGGC CCCGGGGGGT CAACCAGTTC 
GGCAACAAGT ACATCCAGCA GACCAAGCCC CTCACCCTGG AGCGCACCAT TAATCTGTAC 
CCGCTTACCA ATTATACTTT TGGTACAAAG GAGCCCCTCT ATGAGAAGGA CAGCTCTGTT 
GCAGCCAGAT TTCAGCGCAT GAGGGAGGAA TTTGATAAGA TTGGGATGAG AAGGACTGTA 
GAAGGGGTTC TGATTGTTCA TGAACACCGC CTGCCCCACG TGCTCCTGCT GCAGCTGGGG 
ACAACTTTCT TCAAATTACC TGGTGGGGAA CTTAACCCAG GAGAAGATGA AGTTGAAGGA 
CTAAAACGCT TAATGACAGA GATACTTGGT CGTCAAGATG GAGTCCTGCA AGACTGGGTC 
ATTGATGACT GCATTGGGAA CTGGTGGAGA CCAAATTTTG AACCTCCTCA GTATCCGTAT 
ATTCCTGCAC ATATAACAAA ACCCAAGGAA CATAAGAAGT TGTTTCTGGT TCAGCTTCAA 
GAGAAAGCCT TGTTTGCAGT CCCTAAAAAT TACAAGCTGG TAGCTGCACC ATTGTTTGAG 
CTGTATGACA ATGCACCGGG GTATGGACCC ATCATTTCTA GTCTTCCTCA GCTGCTGAGC 
AGGTTCAATT TTATATACAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_080899.1
CDS	148..831
Translation	MSVVPPNRSQTGWPRGVNQFGNKYIQQTKPLTLERTINLYPLTNYTFGTKEPLYEKDSSVAARFQRMREEFDKIGMRRTVEGVLIVHEHRLPHVLLLQLGTTFFKLPGGELNPGEDEVEGLKRLMTEILGRQDGVLQDWVIDDCIGNWWRPNFEPPQYPYIPAHITKPKEHKKLFLVQLQEKALFAVPKNYKLVAAPLFELYDNAPGYGPIISSLPQLLSRFNFIYN

Target ORF information:

RefSeq Version	NM_026623.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus nudix (nucleoside diphosphate linked moiety X)-type motif 21 (Nudt21), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_026623.3

ORF Insert Sequence:

ATGTCTGTGG TGCCGCCCAA TCGCTCGCAG ACGGGCTGGC CCCGGGGGGT CAACCAGTTC 
GGCAACAAGT ACATCCAGCA GACCAAGCCC CTCACCCTGG AGCGCACCAT TAATCTGTAC 
CCGCTTACCA ATTATACTTT TGGTACAAAG GAGCCCCTCT ATGAGAAGGA CAGCTCTGTT 
GCAGCCAGAT TTCAGCGCAT GAGGGAGGAA TTTGATAAGA TTGGGATGAG AAGGACTGTA 
GAAGGGGTTC TGATTGTTCA TGAACACCGC CTGCCCCACG TGCTCCTGCT GCAGCTGGGG 
ACAACTTTCT TCAAATTACC TGGTGGGGAA CTTAACCCAG GAGAAGATGA AGTTGAAGGA 
CTAAAACGCT TAATGACAGA GATACTTGGT CGTCAAGATG GAGTCCTGCA AGACTGGGTC 
ATTGATGACT GCATTGGGAA CTGGTGGAGA CCAAATTTTG AACCTCCTCA GTATCCGTAT 
ATTCCTGCAC ATATAACAAA ACCCAAGGAA CATAAGAAGT TGTTTCTGGT TCAGCTTCAA 
GAGAAAGCCT TGTTTGCAGT CCCTAAAAAT TACAAGCTGG TAGCTGCACC ATTGTTTGAG 
CTGTATGACA ATGCACCGGG GTATGGACCC ATCATTTCTA GTCTTCCTCA GCTGCTGAGC 
AGGTTCAATT TTATATACAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Nudt21 Controls Cell Fate by Connecting Alternative Polyadenylation to Chromatin Signaling.
Cell172(1-2)106-120.e21(2018 Jan)
Brumbaugh J,Di Stefano B,Wang X,Borkent M,Forouzmand E,Clowers KJ,Ji F,Schwarz BA,Kalocsay M,Elledge SJ,Chen Y,Sadreyev RI,Gygi SP,Hu G,Shi Y,Hochedlinger K

Pre-messenger RNA cleavage factor I (CFIm): potential role in alternative polyadenylation during spermatogenesis.
Biology of reproduction78(3)472-82(2008 Mar)
Sartini BL,Wang H,Wang W,Millette CF,Kilpatrick DL

A genome-wide in situ hybridization map of RNA-binding proteins reveals anatomically restricted expression in the developing mouse brain.
BMC developmental biology514(2005 Jul)
McKee AE,Minet E,Stern C,Riahi S,Stiles CD,Silver PA

Interaction of poly(A) polymerase with the 25-kDa subunit of cleavage factor I.
Biochemical and biophysical research communications289(2)513-8(2001 Nov)
Kim H,Lee Y