Ncbp2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ncbp2
Entrez Gene ID	68092
Full Name	nuclear cap binding protein subunit 2
Synonyms	20kDa,5930413E18Rik,AA536802,AI843301,C79367
General protein information	Preferred Names nuclear cap binding protein subunit 2 Names nuclear cap-binding protein subunit 2 20 kDa nuclear cap-binding protein CBP20 NCBP 20 kDa subunit nuclear cap binding protein subunit 2, 20kDa
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 16 Map Location: 16\|16 B2

mRNA and Protein(s)

mRNA	Protein	Name
NM_026554.4	NP_080830.1	nuclear cap-binding protein subunit 2

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	ncbp2	NP_001006879.1
Pan troglodytes (chimpanzee)	NCBP2	XP_516977.2
Rattus norvegicus (Norway rat)	LOC100911481	XP_003751123.2
Homo sapiens (human)	NCBP2	NP_031388.2
Bos taurus (cattle)	NCBP2	NP_001029568.1
Mus musculus (house mouse)	Ncbp2	NP_080830.1
Danio rerio (zebrafish)	ncbp2	NP_775356.1
	NCBP2	XP_002803120.1
Rattus norvegicus (Norway rat)	Pigz	NP_001102995.1
Gallus gallus (chicken)	NCBP2	NP_001264384.1
Oryza sativa (rice)	Os02g0612300	NP_001047412.1
Canis lupus familiaris (dog)	NCBP2	XP_005639661.1
Arabidopsis thaliana (thale cress)	CBP20	NP_001078705.1

Gene Ontology
Bibliography

Related articles in PubMed

Widespread and dynamic translational control of red blood cell development.
Alvarez-Dominguez JR, Zhang X, Hu W
Blood129(5)619-629(2017 02)

mRNA export through an additional cap-binding complex consisting of NCBP1 and NCBP3.
Gebhardt A, Habjan M, Benda C, Meiler A, Haas DA, Hein MY, Mann A, Mann M, Habermann B, Pichlmair A
Nature communications68192(2015 Sep)

Identification of phosphoproteins and their phosphorylation sites in the WEHI-231 B lymphoma cell line.
Shu H, Chen S, Bi Q, Mumby M, Brekken DL
Molecular & cellular proteomics : MCP3(3)279-86(2004 Mar)

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE
Nucleic acids research31(1)278-81(2003 Jan)

PHAX, a mediator of U snRNA nuclear export whose activity is regulated by phosphorylation.
Ohno M, Segref A, Bachi A, Wilm M, Mattaj IW
Cell101(2)187-98(2000 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

NCBP2 is not required for cell viability and poly(A) RNA export.
Title: mRNA export through an additional cap-binding complex consisting of NCBP1 and NCBP3.

The following Ncbp2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ncbp2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu02562	NM_026554.4 Latest version!	Mus musculus nuclear cap binding protein subunit 2 (Ncbp2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ncbp2 ( NM_026554.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_080830.1 Mus musculus nuclear cap binding protein subunit 2 (Ncbp2), mRNA.

Price & Availability↑

CloneID	OMu02562
Clone ID Related Accession (Same CDS sequence)	NM_026554.4
Accession Version	NM_026554.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	nuclear cap-binding protein subunit 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY035864.1, AK077841.1, AK147027.1 and AI843301.1. On Apr 15, 2009 this sequence version replaced NM_026554.3. ##Evidence-Data-START## Transcript exon combination :: AK147027.1, SRR1660811.120494.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGGGTG GCTTACTGAA GGCGCTGCGC AGCGACTCCT ACGTGGAACT CAGCGAGTAC 
CGGGACCAGC ACTTTCGGGG CGACAATGAA GAACAAGAAA AGTTACTGAA GAAAAGCTGT 
ACGTTGTATG TTGGAAATCT TTCCTTTTAT ACAACTGAAG AACAGATTTA TGAACTCTTC 
AGCAAAAGTG GTGATATAAA GAAGATCATC ATGGGTCTGG ATAAAATGAA GAAAACAGCA 
TGTGGGTTCT GTTTTGTGGA ATACTATTCA AGAGCAGATG CGGAGAACGC AATGCGGTAC 
ATAAACGGAA CTCGTCTGGA TGACCGGATC ATACGGACAG ACTGGGATGC AGGCTTTAAG 
GAGGGCAGGC AGTATGGACG TGGACGGTCT GGGGGTCAGG TTCGGGACGA GTATCGGGAG 
GACTACGATG CTGGAAGAGG CGGCTATGGG AAACTGGCAC AAAAACAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_080830.1
CDS	105..575
Translation	MSGGLLKALRSDSYVELSEYRDQHFRGDNEEQEKLLKKSCTLYVGNLSFYTTEEQIYELFSKSGDIKKIIMGLDKMKKTACGFCFVEYYSRADAENAMRYINGTRLDDRIIRTDWDAGFKEGRQYGRGRSGGQVRDEYREDYDAGRGGYGKLAQKQ

Target ORF information:

RefSeq Version	NM_026554.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus nuclear cap binding protein subunit 2 (Ncbp2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_026554.4

ORF Insert Sequence:

ATGTCGGGTG GCTTACTGAA GGCGCTGCGC AGCGACTCCT ACGTGGAACT CAGCGAGTAC 
CGGGACCAGC ACTTTCGGGG CGACAATGAA GAACAAGAAA AGTTACTGAA GAAAAGCTGT 
ACGTTGTATG TTGGAAATCT TTCCTTTTAT ACAACTGAAG AACAGATTTA TGAACTCTTC 
AGCAAAAGTG GTGATATAAA GAAGATCATC ATGGGTCTGG ATAAAATGAA GAAAACAGCA 
TGTGGGTTCT GTTTTGTGGA ATACTATTCA AGAGCAGATG CGGAGAACGC AATGCGGTAC 
ATAAACGGAA CTCGTCTGGA TGACCGGATC ATACGGACAG ACTGGGATGC AGGCTTTAAG 
GAGGGCAGGC AGTATGGACG TGGACGGTCT GGGGGTCAGG TTCGGGACGA GTATCGGGAG 
GACTACGATG CTGGAAGAGG CGGCTATGGG AAACTGGCAC AAAAACAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Widespread and dynamic translational control of red blood cell development.
Blood129(5)619-629(2017 02)
Alvarez-Dominguez JR,Zhang X,Hu W

mRNA export through an additional cap-binding complex consisting of NCBP1 and NCBP3.
Nature communications68192(2015 Sep)
Gebhardt A,Habjan M,Benda C,Meiler A,Haas DA,Hein MY,Mann A,Mann M,Habermann B,Pichlmair A

Identification of phosphoproteins and their phosphorylation sites in the WEHI-231 B lymphoma cell line.
Molecular & cellular proteomics : MCP3(3)279-86(2004 Mar)
Shu H,Chen S,Bi Q,Mumby M,Brekken DL

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Nucleic acids research31(1)278-81(2003 Jan)
Stryke D,Kawamoto M,Huang CC,Johns SJ,King LA,Harper CA,Meng EC,Lee RE,Yee A,L'Italien L,Chuang PT,Young SG,Skarnes WC,Babbitt PC,Ferrin TE

PHAX, a mediator of U snRNA nuclear export whose activity is regulated by phosphorylation.
Cell101(2)187-98(2000 Apr)
Ohno M,Segref A,Bachi A,Wilm M,Mattaj IW