Mid1ip1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mid1ip1
Entrez Gene ID	68041
Full Name	Mid1 interacting protein 1 (gastrulation specific G12-like (zebrafish))
Synonyms	3110038L01Rik,Mig12,S14R
General protein information	Preferred Names Mid1 interacting protein 1 (gastrulation specific G12-like (zebrafish)) Names mid1-interacting protein 1 gastrulation-specific G12-like protein mid1-interacting G12-like protein protein STRAIT11499 homolog spot 14-R spot 14-related protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: X Map Location: X\|X A1.1

mRNA and Protein(s)

mRNA	Protein	Name
NM_026524.4	NP_080800.1	mid1-interacting protein 1
NM_001166635.1	NP_001160107.1	mid1-interacting protein 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	MID1IP1	NP_067065.1
Pan troglodytes (chimpanzee)	MID1IP1	XP_521007.2
	MID1IP1	XP_001084331.1
Gallus gallus (chicken)	MID1IP1	NP_998740.1
Danio rerio (zebrafish)	mid1ip1b	NP_957126.1
Rattus norvegicus (Norway rat)	Mid1ip1	NP_996833.1
Canis lupus familiaris (dog)	MID1IP1	XP_548950.1
Mus musculus (house mouse)	Mid1ip1	NP_001160107.1
Xenopus tropicalis (tropical clawed frog)	mid1ip1	NP_989004.1
Bos taurus (cattle)	MID1IP1	NP_001069851.1

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

Identification of MIG12 as a mediator for stimulation of lipogenesis by LXR activation.
Inoue J, Yamasaki K, Ikeuchi E, Satoh S, Fujiwara Y, Nishimaki-Mogami T, Shimizu M, Sato R
Molecular endocrinology (Baltimore, Md.)25(6)995-1005(2011 Jun)

Crystal structure of Spot 14, a modulator of fatty acid synthesis.
Colbert CL, Kim CW, Moon YA, Henry L, Palnitkar M, McKean WB, Fitzgerald K, Deisenhofer J, Horton JD, Kwon HJ
Proceedings of the National Academy of Sciences of the United States of America107(44)18820-5(2010 Nov)

Induced polymerization of mammalian acetyl-CoA carboxylase by MIG12 provides a tertiary level of regulation of fatty acid synthesis.
Kim CW, Moon YA, Park SW, Cheng D, Kwon HJ, Horton JD
Proceedings of the National Academy of Sciences of the United States of America107(21)9626-31(2010 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

MIG12 is a mediator for stimulation of lipogenesis by LXR activation in the liver
Title: Identification of MIG12 as a mediator for stimulation of lipogenesis by LXR activation.

the S14-R protein is a compensatory factor, at least partially responsible for the normal liver lipogenesis observed in the S14 null mouse
Title: Hepatic expression of the SPOT 14 (S14) paralog S14-related (Mid1 interacting protein) is regulated by dietary carbohydrate.

Mig12 is expressed in the embryonic ventral midline and co-operates with Mid1 to stabilize microtubules
Title: Mig12, a novel Opitz syndrome gene product partner, is expressed in the embryonic ventral midline and co-operates with Mid1 to bundle and stabilize microtubules.

The following Mid1ip1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mid1ip1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu02504	NM_001166635.1 Latest version!	Mus musculus Mid1 interacting protein 1 (gastrulation specific G12-like (zebrafish)) (Mid1ip1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu02504	NM_026524.4 Latest version!	Mus musculus Mid1 interacting protein 1 (gastrulation specific G12-like (zebrafish)) (Mid1ip1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mid1ip1 ( NM_026524.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_080800.1 Mus musculus Mid1 interacting protein 1 (gastrulation specific G12-like (zebrafish)) (Mid1ip1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu02504
Clone ID Related Accession (Same CDS sequence)	NM_026524.4 , NM_001166635.1
Accession Version	NM_026524.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Mus musculus(house mouse)
Product	mid1-interacting protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL671335.12. On Oct 30, 2009 this sequence version replaced NM_026524.3. Transcript Variant: This variant (2) uses a different splice site in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: CJ156837.1, AK160062.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGATGCAAA TCTGCGACAC ATATAACCAG AAGCACTCGC TCTTTAACGC CATGAATCGC 
TTCATTGGCG CGGTGAACAA CATGGACCAG ACGGTGATGG TGCCCAGTCT GCTGCGCGAC 
GTACCCCTGT CCGAGCCGGA GATAGACGAG GTCAGCGTGG AGGTAGGCGG CAGTGGCGGC 
TGCCTGGAGG AGCGCACGAC CCCGGCCCCA AGCCCGGGCA GCGCCAATGA AAGCTTTTTC 
GCGCCCTCCC GGGACATGTA CAGCCACTAC GTGCTGCTCA AGTCCATCCG CAATGATATC 
GAGTGGGGAG TCCTGCACCA GCCTTCGTCT CCGCCGGCCG GGAGCGAGGA GAGCACCTGG 
AAGCCCAAGG ACATCCTGGT GGGCCTGAGT CACTTGGAGA GCGCGGATGC GGGCGAGGAA 
GATCTGGAGC AGCAGTTCCA CTACCACCTG CGCGGGCTGC ACACCGTGCT CTCCAAACTC 
ACCCGAAAAG CCAACATCCT CACCAATAGA TACAAGCAGG AGATCGGCTT CAGTAATTGG 
GGCCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_080800.1
CDS	584..1132
Translation	MMQICDTYNQKHSLFNAMNRFIGAVNNMDQTVMVPSLLRDVPLSEPEIDEVSVEVGGSGGCLEERTTPAPSPGSANESFFAPSRDMYSHYVLLKSIRNDIEWGVLHQPSSPPAGSEESTWKPKDILVGLSHLESADAGEEDLEQQFHYHLRGLHTVLSKLTRKANILTNRYKQEIGFSNWGH

Target ORF information:

RefSeq Version	NM_026524.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus Mid1 interacting protein 1 (gastrulation specific G12-like (zebrafish)) (Mid1ip1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_026524.4

ORF Insert Sequence:

ATGATGCAAA TCTGCGACAC ATATAACCAG AAGCACTCGC TCTTTAACGC CATGAATCGC 
TTCATTGGCG CGGTGAACAA CATGGACCAG ACGGTGATGG TGCCCAGTCT GCTGCGCGAC 
GTACCCCTGT CCGAGCCGGA GATAGACGAG GTCAGCGTGG AGGTAGGCGG CAGTGGCGGC 
TGCCTGGAGG AGCGCACGAC CCCGGCCCCA AGCCCGGGCA GCGCCAATGA AAGCTTTTTC 
GCGCCCTCCC GGGACATGTA CAGCCACTAC GTGCTGCTCA AGTCCATCCG CAATGATATC 
GAGTGGGGAG TCCTGCACCA GCCTTCGTCT CCGCCGGCCG GGAGCGAGGA GAGCACCTGG 
AAGCCCAAGG ACATCCTGGT GGGCCTGAGT CACTTGGAGA GCGCGGATGC GGGCGAGGAA 
GATCTGGAGC AGCAGTTCCA CTACCACCTG CGCGGGCTGC ACACCGTGCT CTCCAAACTC 
ACCCGAAAAG CCAACATCCT CACCAATAGA TACAAGCAGG AGATCGGCTT CAGTAATTGG 
GGCCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mid1ip1 ( NM_001166635.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001160107.1 Mus musculus Mid1 interacting protein 1 (gastrulation specific G12-like (zebrafish)) (Mid1ip1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu02504
Clone ID Related Accession (Same CDS sequence)	NM_026524.4 , NM_001166635.1
Accession Version	NM_001166635.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Mus musculus(house mouse)
Product	mid1-interacting protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL671335.12. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK172143.1, AK151153.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGATGCAAA TCTGCGACAC ATATAACCAG AAGCACTCGC TCTTTAACGC CATGAATCGC 
TTCATTGGCG CGGTGAACAA CATGGACCAG ACGGTGATGG TGCCCAGTCT GCTGCGCGAC 
GTACCCCTGT CCGAGCCGGA GATAGACGAG GTCAGCGTGG AGGTAGGCGG CAGTGGCGGC 
TGCCTGGAGG AGCGCACGAC CCCGGCCCCA AGCCCGGGCA GCGCCAATGA AAGCTTTTTC 
GCGCCCTCCC GGGACATGTA CAGCCACTAC GTGCTGCTCA AGTCCATCCG CAATGATATC 
GAGTGGGGAG TCCTGCACCA GCCTTCGTCT CCGCCGGCCG GGAGCGAGGA GAGCACCTGG 
AAGCCCAAGG ACATCCTGGT GGGCCTGAGT CACTTGGAGA GCGCGGATGC GGGCGAGGAA 
GATCTGGAGC AGCAGTTCCA CTACCACCTG CGCGGGCTGC ACACCGTGCT CTCCAAACTC 
ACCCGAAAAG CCAACATCCT CACCAATAGA TACAAGCAGG AGATCGGCTT CAGTAATTGG 
GGCCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001160107.1
CDS	587..1135
Translation	MMQICDTYNQKHSLFNAMNRFIGAVNNMDQTVMVPSLLRDVPLSEPEIDEVSVEVGGSGGCLEERTTPAPSPGSANESFFAPSRDMYSHYVLLKSIRNDIEWGVLHQPSSPPAGSEESTWKPKDILVGLSHLESADAGEEDLEQQFHYHLRGLHTVLSKLTRKANILTNRYKQEIGFSNWGH

Target ORF information:

RefSeq Version	NM_001166635.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Mid1 interacting protein 1 (gastrulation specific G12-like (zebrafish)) (Mid1ip1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001166635.1

ORF Insert Sequence:

ATGATGCAAA TCTGCGACAC ATATAACCAG AAGCACTCGC TCTTTAACGC CATGAATCGC 
TTCATTGGCG CGGTGAACAA CATGGACCAG ACGGTGATGG TGCCCAGTCT GCTGCGCGAC 
GTACCCCTGT CCGAGCCGGA GATAGACGAG GTCAGCGTGG AGGTAGGCGG CAGTGGCGGC 
TGCCTGGAGG AGCGCACGAC CCCGGCCCCA AGCCCGGGCA GCGCCAATGA AAGCTTTTTC 
GCGCCCTCCC GGGACATGTA CAGCCACTAC GTGCTGCTCA AGTCCATCCG CAATGATATC 
GAGTGGGGAG TCCTGCACCA GCCTTCGTCT CCGCCGGCCG GGAGCGAGGA GAGCACCTGG 
AAGCCCAAGG ACATCCTGGT GGGCCTGAGT CACTTGGAGA GCGCGGATGC GGGCGAGGAA 
GATCTGGAGC AGCAGTTCCA CTACCACCTG CGCGGGCTGC ACACCGTGCT CTCCAAACTC 
ACCCGAAAAG CCAACATCCT CACCAATAGA TACAAGCAGG AGATCGGCTT CAGTAATTGG 
GGCCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

Identification of MIG12 as a mediator for stimulation of lipogenesis by LXR activation.
Molecular endocrinology (Baltimore, Md.)25(6)995-1005(2011 Jun)
Inoue J,Yamasaki K,Ikeuchi E,Satoh S,Fujiwara Y,Nishimaki-Mogami T,Shimizu M,Sato R

Crystal structure of Spot 14, a modulator of fatty acid synthesis.
Proceedings of the National Academy of Sciences of the United States of America107(44)18820-5(2010 Nov)
Colbert CL,Kim CW,Moon YA,Henry L,Palnitkar M,McKean WB,Fitzgerald K,Deisenhofer J,Horton JD,Kwon HJ

Induced polymerization of mammalian acetyl-CoA carboxylase by MIG12 provides a tertiary level of regulation of fatty acid synthesis.
Proceedings of the National Academy of Sciences of the United States of America107(21)9626-31(2010 May)
Kim CW,Moon YA,Park SW,Cheng D,Kwon HJ,Horton JD

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Mid1ip1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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