Pclaf cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pclaf
Entrez Gene ID	68026
Full Name	PCNA clamp associated factor
Synonyms	2810417H13Rik,AA409629,Ns5apt9,Ns5atp9,PAF15,Paf,mKIAA0101,p15(PAF)
General protein information	Preferred Names PCNA clamp associated factor Names PCNA-associated factor HCV NS5A-transactivated protein 9 homolog PCNA-associated factor of 15 kDa p15PAF
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9\|9 C

mRNA and Protein(s)

mRNA	Protein	Name
NM_026515.2	NP_080791.2	PCNA-associated factor

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	kiaa0101	NP_001005130.1
Homo sapiens (human)	KIAA0101	NP_055551.1
Bos taurus (cattle)	KIAA0101	NP_001015678.1
	KIAA0101	XP_002804860.1
Mus musculus (house mouse)	2810417H13Rik	NP_080791.2
Pan troglodytes (chimpanzee)	KIAA0101	XP_003952718.1
Rattus norvegicus (Norway rat)	Ns5atp9	NP_958821.1
Danio rerio (zebrafish)	LOC100333229	XP_002666801.2

Gene Ontology
Bibliography

Related articles in PubMed

Ubiquitome Analysis Reveals PCNA-Associated Factor 15 (PAF15) as a Specific Ubiquitination Target of UHRF1 in Embryonic Stem Cells.
Karg E, Smets M, Ryan J, Forn? I, Qin W, Mulholland CB, Kalideris G, Imhof A, Bultmann S, Leonhardt H
Journal of molecular biology429(24)3814-3824(2017 Dec)

Cellular heterogeneity in the ureteric progenitor niche and distinct profiles of branching morphogenesis in organ development.
Rutledge EA, Benazet JD, McMahon AP
Development (Cambridge, England)144(17)3177-3188(2017 09)

Repression of Interstitial Identity in Nephron Progenitor Cells by Pax2 Establishes the Nephron-Interstitium Boundary during Kidney Development.
Naiman N, Fujioka K, Fujino M, Valerius MT, Potter SS, McMahon AP, Kobayashi A
Developmental cell41(4)349-365.e3(2017 05)

PAF-Wnt signaling-induced cell plasticity is required for maintenance of breast cancer cell stemness.
Wang X, Jung YS, Jun S, Lee S, Wang W, Schneider A, Sun Oh Y, Lin SH, Park BJ, Chen J, Keyomarsi K, Park JI
Nature communications710633(2016 Feb)

PAF-mediated MAPK signaling hyperactivation via LAMTOR3 induces pancreatic tumorigenesis.
Jun S, Lee S, Kim HC, Ng C, Schneider AM, Ji H, Ying H, Wang H, DePinho RA, Park JI
Cell reports5(2)314-22(2013 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

UHRF1 ubiquitinates PAF15 at Lys 15 and Lys 24 and promotes its binding to PCNA during late S-phase
Title: Ubiquitome Analysis Reveals PCNA-Associated Factor 15 (PAF15) as a Specific Ubiquitination Target of UHRF1 in Embryonic Stem Cells.

Results unveil an unsuspected role of the PAF-Wnt signalling axis in modulating cell plasticity, which is required for the maintenance of breast cancer cell stemness.
Title: PAF-Wnt signaling-induced cell plasticity is required for maintenance of breast cancer cell stemness.

PAF-induced ductal epithelial cell hyperproliferation was accompanied by extracellular signal-regulated kinase (ERK) phosphorylation independently of Ras or Raf mutations.
Title: PAF-mediated MAPK signaling hyperactivation via LAMTOR3 induces pancreatic tumorigenesis.

Paf as a novel and essential regulator of early hematopoiesis.
Title: The Paf oncogene is essential for hematopoietic stem cell function and development.

highly restricted expression of the p15(PAF) gene in a spatio-temporal manner during embryogenesis
Title: Murine embryonic expression of the gene for the UV-responsive protein p15(PAF).

The following Pclaf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pclaf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu14621	NM_026515.2 Latest version!	Mus musculus PCNA clamp associated factor (Pclaf), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pclaf ( NM_026515.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_080791.2 Mus musculus PCNA clamp associated factor (Pclaf), mRNA.

Price & Availability↑

CloneID	OMu14621
Clone ID Related Accession (Same CDS sequence)	NM_026515.2
Accession Version	NM_026515.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	PCNA-associated factor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK011090.1, AK011835.1 and AC112676.8. On or before Dec 7, 2005 this sequence version replaced XM_925450.1, XM_918050.1, NM_026515.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK013105.1, AK011835.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849382 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGCGGA CCAAAGCAAA CTACGTTCCA GGAGCCTACA GAAAAGCGGT GGCTTCTCAA 
GCCCCTAGGA AGGTGCTTGG CTCCTCCACC TTTGTCACCA ATTCTTCAAG TTCGTCGAGA 
AAAGCTGAAA ATAAGTATGC AGGAGGGAAC CCAGTCTGTG TGCGCCCAAC TCCCAAGTGG 
CAAAAAGGCA TCGGGGAATT CTTCAGGCTG TCCCCTAAAG AGTCTAAAAA GGAAAACCAG 
GCTCCTGAAG AAGCAGGAAC CAGTGGCTTA GGAAAAGCAA AGAGAAAAGC TTGTCCTTTG 
CAACCTGATC ACAGAGATGA TGAAAACGAA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_080791.2
CDS	107..439
Translation	MVRTKANYVPGAYRKAVASQAPRKVLGSSTFVTNSSSSSRKAENKYAGGNPVCVRPTPKWQKGIGEFFRLSPKESKKENQAPEEAGTSGLGKAKRKACPLQPDHRDDENE

Target ORF information:

RefSeq Version	NM_026515.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus PCNA clamp associated factor (Pclaf), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_026515.2

ORF Insert Sequence:

ATGGTGCGGA CCAAAGCAAA CTACGTTCCA GGAGCCTACA GAAAAGCGGT GGCTTCTCAA 
GCCCCTAGGA AGGTGCTTGG CTCCTCCACC TTTGTCACCA ATTCTTCAAG TTCGTCGAGA 
AAAGCTGAAA ATAAGTATGC AGGAGGGAAC CCAGTCTGTG TGCGCCCAAC TCCCAAGTGG 
CAAAAAGGCA TCGGGGAATT CTTCAGGCTG TCCCCTAAAG AGTCTAAAAA GGAAAACCAG 
GCTCCTGAAG AAGCAGGAAC CAGTGGCTTA GGAAAAGCAA AGAGAAAAGC TTGTCCTTTG 
CAACCTGATC ACAGAGATGA TGAAAACGAA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Ubiquitome Analysis Reveals PCNA-Associated Factor 15 (PAF15) as a Specific Ubiquitination Target of UHRF1 in Embryonic Stem Cells.
Journal of molecular biology429(24)3814-3824(2017 Dec)
Karg E,Smets M,Ryan J,Forn? I,Qin W,Mulholland CB,Kalideris G,Imhof A,Bultmann S,Leonhardt H

Cellular heterogeneity in the ureteric progenitor niche and distinct profiles of branching morphogenesis in organ development.
Development (Cambridge, England)144(17)3177-3188(2017 09)
Rutledge EA,Benazet JD,McMahon AP

Repression of Interstitial Identity in Nephron Progenitor Cells by Pax2 Establishes the Nephron-Interstitium Boundary during Kidney Development.
Developmental cell41(4)349-365.e3(2017 05)
Naiman N,Fujioka K,Fujino M,Valerius MT,Potter SS,McMahon AP,Kobayashi A

PAF-Wnt signaling-induced cell plasticity is required for maintenance of breast cancer cell stemness.
Nature communications710633(2016 Feb)
Wang X,Jung YS,Jun S,Lee S,Wang W,Schneider A,Sun Oh Y,Lin SH,Park BJ,Chen J,Keyomarsi K,Park JI

PAF-mediated MAPK signaling hyperactivation via LAMTOR3 induces pancreatic tumorigenesis.
Cell reports5(2)314-22(2013 Oct)
Jun S,Lee S,Kim HC,Ng C,Schneider AM,Ji H,Ying H,Wang H,DePinho RA,Park JI