Snx2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Snx2
Entrez Gene ID	67804
Full Name	sorting nexin 2
Synonyms	0610030A03Rik
General protein information	Preferred Names sorting nexin 2 Names sorting nexin-2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 18 Map Location: 18\|18 D1

mRNA and Protein(s)

mRNA	Protein	Name
NM_026386.1	NP_080662.1	sorting nexin-2
XM_006526190.3	XP_006526253.2	sorting nexin-2 isoform X1
XM_030250577.1	XP_030106437.1	sorting nexin-2 isoform X1
NM_001357463.1	NP_001344392.1	sorting nexin-2 isoform 2
NM_026386.2	NP_080662.1	sorting nexin-2 isoform 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	SNX2	NP_003091.2
Mus musculus (house mouse)	Snx2	NP_080662.1
Caenorhabditis elegans (roundworm)	snx-1	NP_508216.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009255	XP_320042.3
Magnaporthe oryzae (rice blast fungus)	MGG_06743	XP_003709457.1
Rattus norvegicus (Norway rat)	Snx2	NP_001099605.2
Gallus gallus (chicken)	SNX2	NP_001012625.1
	SNX2	XP_001092124.1
Bos taurus (cattle)	SNX2	NP_001033608.1
Danio rerio (zebrafish)	snx2	NP_001032787.1
Drosophila melanogaster (fruit fly)	Snx1	NP_608777.1
Neurospora crassa	NCU04137	XP_961293.2
Xenopus tropicalis (tropical clawed frog)	snx2	XP_004910540.1
Pan troglodytes (chimpanzee)	SNX2	XP_001154033.1
Canis lupus familiaris (dog)	SNX2	XP_531886.3

Gene Ontology
Bibliography

Related articles in PubMed

Biogenesis of lysosome-related organelles complex-1 subunit 1 (BLOS1) interacts with sorting nexin 2 and the endosomal sorting complex required for transport-I (ESCRT-I) component TSG101 to mediate the sorting of epidermal growth factor receptor into endosomal compartments.
Zhang A, He X, Zhang L, Yang L, Woodman P, Li W
The Journal of biological chemistry289(42)29180-94(2014 Oct)

Visceral endoderm expression of Yin-Yang1 (YY1) is required for VEGFA maintenance and yolk sac development.
Rhee S, Guerrero-Zayas MI, Wallingford MC, Ortiz-Pineda P, Mager J, Tremblay KD
PloS one8(3)e58828(2013)

Loss of the PlagL2 transcription factor affects lacteal uptake of chylomicrons.
Van Dyck F, Braem CV, Chen Z, Declercq J, Deckers R, Kim BM, Ito S, Wu MK, Cohen DE, Dewerchin M, Derua R, Waelkens E, Fiette L, Roebroek A, Schuit F, Van de Ven WJ, Shivdasani RA
Cell metabolism6(5)406-13(2007 Nov)

Genetic evidence for a mammalian retromer complex containing sorting nexins 1 and 2.
Griffin CT, Trejo J, Magnuson T
Proceedings of the National Academy of Sciences of the United States of America102(42)15173-7(2005 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

BLOS1 interacts with sorting nexin 2 and the ESCRT-I component TSG101 to mediate the sorting of EGFR into endosomal compartments
Title: Biogenesis of lysosome-related organelles complex-1 subunit 1 (BLOS1) interacts with sorting nexin 2 and the endosomal sorting complex required for transport-I (ESCRT-I) component TSG101 to mediate the sorting of epidermal growth factor receptor into endosomal compartments.

Data describe a functional interaction between Hbeta58, sorting nexin 1 (SNX1), and SNX2 and strongly suggest that SNX2 plays a more critical role than SNX1 in retromer activity during embryonic development.
Title: Genetic evidence for a mammalian retromer complex containing sorting nexins 1 and 2.

Data show that mice lacking sorting nexin 1 or sorting nexin 2 are viable and fertile, whereas embryos deficient in both proteins arrest at midgestation.
Title: Genetic analysis of sorting nexins 1 and 2 reveals a redundant and essential function in mice.

The following Snx2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Snx2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu02156	NM_026386.2 Latest version!	Mus musculus sorting nexin 2 (Snx2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$300.30 $429.00
OMu70192	NM_001357463.1 Latest version!	Mus musculus sorting nexin 2 (Snx2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu70192	XM_006526190.3 Latest version!	Mus musculus sorting nexin 2 (Snx2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu87425	XM_030250577.1 Latest version!	Mus musculus sorting nexin 2 (Snx2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OMu02156	NM_026386.1	Mus musculus sorting nexin 2 (Snx2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$300.30 $429.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Snx2 ( NM_026386.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_080662.1 Mus musculus sorting nexin 2 (Snx2), mRNA.

Price & Availability↑

CloneID	OMu02156
Clone ID Related Accession (Same CDS sequence)	NM_026386.1 , NM_026386.2
Accession Version	NM_026386.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1560bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-24
Organism	Mus musculus(House mouse)
Product	sorting nexin-2
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AK002692.1. ##Evidence-Data-START## Transcript exon combination :: AK002692.1, AK075929.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGCCG AGAGGGAGCC TCCTCCGCTG GGGGACGTGA AGCCCACAGA CTTTGAGGAG 
CTGGAGGACG GAGAGGACCT GTTCACCAGC ACCGTCTCCA CCCTTGAGTC AAGTCCATCA 
TCTCCCGAAC CAGCCAGTCT CCCTGCAGAA GATATCAGTG CAAACTCTAA TGGCTCCAAA 
CCTGTAGAAG TTGTCCTAGA CGATGACAGA GAAGATCTTT TCGCAGAAGC CACAGAAGAG 
GTGTCCTTGG ACAGTCCCGA AAGAGAGCTC ATCCTGTCCT CCGAGCCGTC TCCTGCGGTC 
ACCCCTGTCA CTCCCACCAC ACTCATTGCT CCTAGAATCG AATCAAAGAG TATCTCTGCT 
CCAGTCATCT TTGACAGATC CAGGGACGAG ATTGAAGAAG AAGCAAATGG AGATATTTTT 
GATATAGAAA TTGGTGTGTC AGATCCGGAA AAAGTTGGTG ATGGCATGAA TGCTTACATG 
GCATATAGAG TAACAACAAA GACTTCTCTT TCCATGTTCA GTAAGAGTGA ATTTTCAGTC 
AAAAGAAGAT TCAGTGACTT TCTTGGTTTG CATAGCAAAT TAGCAAGCAA ATATTTACAT 
GTTGGTTACA TCGTGCCACC CGCTCCAGAA AAGAGTATAG TAGGTATGAC CAAGGTCAAA 
GTAGGTAAAG AAGATTCATC ATCCACTGAG TTTGTAGAAA AACGAAGAGC GGCACTGGAG 
AGGTATCTTC AGAGAACAGT AAAGCATCCG ACATTGCTTC AGGATCCTGA CTTAAGGCAG 
TTTTTGGAAA GCTCAGAGCT GCCGAGAGCA GTTAATACGC AGGCTCTGAG TGGAGCAGGA 
ATATTGAGGA TGGTGAACAA GGCTGCCGAC GCTGTCAACA AAATGACAAT CAAGATGAAT 
GAATCGGATG CATGGTTTGA AGAAAAGCAA CAGCAATTTG AGAATCTTGA CCAGCAACTT 
CGGAAACTTC ATGCCAGTGT TGAAGCCTTG GTCTGTCATA GAAAAGAACT TTCAGCCAAC 
ACAGCCGCCT TTGCTAAGAG TGCTGCCATG CTAGGTAATT CTGAGGACCA CACTGCGCTC 
TCTAGAGCTT TGTCTCAGCT GGCAGAGGTT GAGGAGAAGA TAGACCAGTT ACATCAAGAG 
CAAGCTTTTG CTGACTTTTA CATGTTCTCA GAACTTCTTA GTGACTACAT TCGTCTCATT 
GCTGCAGTGA AAGGTGTGTT TGACCATCGG ATGAAGTGCT GGCAGAAATG GGAAGATGCT 
CAAATTACCT TGCTCAAAAA ACGTGAAACT GAGGCAAAAA TGATGGTTGC TAACAAGCCA 
GATAAAATTC AACAAGCTAA AAACGAGATA AGAGAGTGGG AGGCGAAAGT ACAACAAGGA 
GAGAGAGATT TTGAGCAGAT CTCTAAAACA ATTCGAAAAG AAGTGGGAAG ATTTGAGAAA 
GAACGAGTGA AAGATTTTAA AGCTGTTATC ATCAAGTACT TGGAGTCATT AGTACAAACA 
CAGCAACAGC TGATAAAGTA CTGGGAGGCA TTCCTACCTG AAGCCAAAGC CATTGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_080662.1
CDS	16..1575
Translation	MAAEREPPPLGDVKPTDFEELEDGEDLFTSTVSTLESSPSSPEPASLPAEDISANSNGSKPVEVVLDDDREDLFAEATEEVSLDSPERELILSSEPSPAVTPVTPTTLIAPRIESKSISAPVIFDRSRDEIEEEANGDIFDIEIGVSDPEKVGDGMNAYMAYRVTTKTSLSMFSKSEFSVKRRFSDFLGLHSKLASKYLHVGYIVPPAPEKSIVGMTKVKVGKEDSSSTEFVEKRRAALERYLQRTVKHPTLLQDPDLRQFLESSELPRAVNTQALSGAGILRMVNKAADAVNKMTIKMNESDAWFEEKQQQFENLDQQLRKLHASVEALVCHRKELSANTAAFAKSAAMLGNSEDHTALSRALSQLAEVEEKIDQLHQEQAFADFYMFSELLSDYIRLIAAVKGVFDHRMKCWQKWEDAQITLLKKRETEAKMMVANKPDKIQQAKNEIREWEAKVQQGERDFEQISKTIRKEVGRFEKERVKDFKAVIIKYLESLVQTQQQLIKYWEAFLPEAKAIA

Target ORF information:

RefSeq Version	NM_026386.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus sorting nexin 2 (Snx2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_026386.1

ORF Insert Sequence:

ATGGCGGCCG AGAGGGAGCC TCCTCCGCTG GGGGACGTGA AGCCCACAGA CTTTGAGGAG 
CTGGAGGACG GAGAGGACCT GTTCACCAGC ACCGTCTCCA CCCTTGAGTC AAGTCCATCA 
TCTCCCGAAC CAGCCAGTCT CCCTGCAGAA GATATCAGTG CAAACTCTAA TGGCTCCAAA 
CCTGTAGAAG TTGTCCTAGA CGATGACAGA GAAGATCTTT TCGCAGAAGC CACAGAAGAG 
GTGTCCTTGG ACAGTCCCGA AAGAGAGCTC ATCCTGTCCT CCGAGCCGTC TCCTGCGGTC 
ACCCCTGTCA CTCCCACCAC ACTCATTGCT CCTAGAATCG AATCAAAGAG TATCTCTGCT 
CCAGTCATCT TTGACAGATC CAGGGACGAG ATTGAAGAAG AAGCAAATGG AGATATTTTT 
GATATAGAAA TTGGTGTGTC AGATCCGGAA AAAGTTGGTG ATGGCATGAA TGCTTACATG 
GCATATAGAG TAACAACAAA GACTTCTCTT TCCATGTTCA GTAAGAGTGA ATTTTCAGTC 
AAAAGAAGAT TCAGTGACTT TCTTGGTTTG CATAGCAAAT TAGCAAGCAA ATATTTACAT 
GTTGGTTACA TCGTGCCACC CGCTCCAGAA AAGAGTATAG TAGGTATGAC CAAGGTCAAA 
GTAGGTAAAG AAGATTCATC ATCCACTGAG TTTGTAGAAA AACGAAGAGC GGCACTGGAG 
AGGTATCTTC AGAGAACAGT AAAGCATCCG ACATTGCTTC AGGATCCTGA CTTAAGGCAG 
TTTTTGGAAA GCTCAGAGCT GCCGAGAGCA GTTAATACGC AGGCTCTGAG TGGAGCAGGA 
ATATTGAGGA TGGTGAACAA GGCTGCCGAC GCTGTCAACA AAATGACAAT CAAGATGAAT 
GAATCGGATG CATGGTTTGA AGAAAAGCAA CAGCAATTTG AGAATCTTGA CCAGCAACTT 
CGGAAACTTC ATGCCAGTGT TGAAGCCTTG GTCTGTCATA GAAAAGAACT TTCAGCCAAC 
ACAGCCGCCT TTGCTAAGAG TGCTGCCATG CTAGGTAATT CTGAGGACCA CACTGCGCTC 
TCTAGAGCTT TGTCTCAGCT GGCAGAGGTT GAGGAGAAGA TAGACCAGTT ACATCAAGAG 
CAAGCTTTTG CTGACTTTTA CATGTTCTCA GAACTTCTTA GTGACTACAT TCGTCTCATT 
GCTGCAGTGA AAGGTGTGTT TGACCATCGG ATGAAGTGCT GGCAGAAATG GGAAGATGCT 
CAAATTACCT TGCTCAAAAA ACGTGAAACT GAGGCAAAAA TGATGGTTGC TAACAAGCCA 
GATAAAATTC AACAAGCTAA AAACGAGATA AGAGAGTGGG AGGCGAAAGT ACAACAAGGA 
GAGAGAGATT TTGAGCAGAT CTCTAAAACA ATTCGAAAAG AAGTGGGAAG ATTTGAGAAA 
GAACGAGTGA AAGATTTTAA AGCTGTTATC ATCAAGTACT TGGAGTCATT AGTACAAACA 
CAGCAACAGC TGATAAAGTA CTGGGAGGCA TTCCTACCTG AAGCCAAAGC CATTGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Snx2 ( NM_026386.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_080662.1 Mus musculus sorting nexin 2 (Snx2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu02156
Clone ID Related Accession (Same CDS sequence)	NM_026386.1 , NM_026386.2
Accession Version	NM_026386.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1560bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	sorting nexin-2 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC127421.3, AK145840.1, AK002692.1 and AC148020.3. On Oct 28, 2017 this sequence version replaced NM_026386.1. ##Evidence-Data-START## Transcript exon combination :: AK145840.1, AK002692.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGCCG AGAGGGAGCC TCCTCCGCTG GGGGACGTGA AGCCCACAGA CTTTGAGGAG 
CTGGAGGACG GAGAGGACCT GTTCACCAGC ACCGTCTCCA CCCTTGAGTC AAGTCCATCA 
TCTCCCGAAC CAGCCAGTCT CCCTGCAGAA GATATCAGTG CAAACTCTAA TGGCTCCAAA 
CCTGTAGAAG TTGTCCTAGA CGATGACAGA GAAGATCTTT TCGCAGAAGC CACAGAAGAG 
GTGTCCTTGG ACAGTCCCGA AAGAGAGCTC ATCCTGTCCT CCGAGCCGTC TCCTGCGGTC 
ACCCCTGTCA CTCCCACCAC ACTCATTGCT CCTAGAATCG AATCAAAGAG TATCTCTGCT 
CCAGTCATCT TTGACAGATC CAGGGACGAG ATTGAAGAAG AAGCAAATGG AGATATTTTT 
GATATAGAAA TTGGTGTGTC AGATCCGGAA AAAGTTGGTG ATGGCATGAA TGCTTACATG 
GCATATAGAG TAACAACAAA GACTTCTCTT TCCATGTTCA GTAAGAGTGA ATTTTCAGTC 
AAAAGAAGAT TCAGTGACTT TCTTGGTTTG CATAGCAAAT TAGCAAGCAA ATATTTACAT 
GTTGGTTACA TCGTGCCACC CGCTCCAGAA AAGAGTATAG TAGGTATGAC CAAGGTCAAA 
GTAGGTAAAG AAGATTCATC ATCCACTGAG TTTGTAGAAA AACGAAGAGC GGCACTGGAG 
AGGTATCTTC AGAGAACAGT AAAGCATCCG ACATTGCTTC AGGATCCTGA CTTAAGGCAG 
TTTTTGGAAA GCTCAGAGCT GCCGAGAGCA GTTAATACGC AGGCTCTGAG TGGAGCAGGA 
ATATTGAGGA TGGTGAACAA GGCTGCCGAC GCTGTCAACA AAATGACAAT CAAGATGAAT 
GAATCGGATG CATGGTTTGA AGAAAAGCAA CAGCAATTTG AGAATCTTGA CCAGCAACTT 
CGGAAACTTC ATGCCAGTGT TGAAGCCTTG GTCTGTCATA GAAAAGAACT TTCAGCCAAC 
ACAGCCGCCT TTGCTAAGAG TGCTGCCATG CTAGGTAATT CTGAGGACCA CACTGCGCTC 
TCTAGAGCTT TGTCTCAGCT GGCAGAGGTT GAGGAGAAGA TAGACCAGTT ACATCAAGAG 
CAAGCTTTTG CTGACTTTTA CATGTTCTCA GAACTTCTTA GTGACTACAT TCGTCTCATT 
GCTGCAGTGA AAGGTGTGTT TGACCATCGG ATGAAGTGCT GGCAGAAATG GGAAGATGCT 
CAAATTACCT TGCTCAAAAA ACGTGAAACT GAGGCAAAAA TGATGGTTGC TAACAAGCCA 
GATAAAATTC AACAAGCTAA AAACGAGATA AGAGAGTGGG AGGCGAAAGT ACAACAAGGA 
GAGAGAGATT TTGAGCAGAT CTCTAAAACA ATTCGAAAAG AAGTGGGAAG ATTTGAGAAA 
GAACGAGTGA AAGATTTTAA AGCTGTTATC ATCAAGTACT TGGAGTCATT AGTACAAACA 
CAGCAACAGC TGATAAAGTA CTGGGAGGCA TTCCTACCTG AAGCCAAAGC CATTGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_080662.1
CDS	89..1648
Translation	MAAEREPPPLGDVKPTDFEELEDGEDLFTSTVSTLESSPSSPEPASLPAEDISANSNGSKPVEVVLDDDREDLFAEATEEVSLDSPERELILSSEPSPAVTPVTPTTLIAPRIESKSISAPVIFDRSRDEIEEEANGDIFDIEIGVSDPEKVGDGMNAYMAYRVTTKTSLSMFSKSEFSVKRRFSDFLGLHSKLASKYLHVGYIVPPAPEKSIVGMTKVKVGKEDSSSTEFVEKRRAALERYLQRTVKHPTLLQDPDLRQFLESSELPRAVNTQALSGAGILRMVNKAADAVNKMTIKMNESDAWFEEKQQQFENLDQQLRKLHASVEALVCHRKELSANTAAFAKSAAMLGNSEDHTALSRALSQLAEVEEKIDQLHQEQAFADFYMFSELLSDYIRLIAAVKGVFDHRMKCWQKWEDAQITLLKKRETEAKMMVANKPDKIQQAKNEIREWEAKVQQGERDFEQISKTIRKEVGRFEKERVKDFKAVIIKYLESLVQTQQQLIKYWEAFLPEAKAIA

Target ORF information:

RefSeq Version	NM_026386.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus sorting nexin 2 (Snx2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_026386.2

ORF Insert Sequence:

ATGGCGGCCG AGAGGGAGCC TCCTCCGCTG GGGGACGTGA AGCCCACAGA CTTTGAGGAG 
CTGGAGGACG GAGAGGACCT GTTCACCAGC ACCGTCTCCA CCCTTGAGTC AAGTCCATCA 
TCTCCCGAAC CAGCCAGTCT CCCTGCAGAA GATATCAGTG CAAACTCTAA TGGCTCCAAA 
CCTGTAGAAG TTGTCCTAGA CGATGACAGA GAAGATCTTT TCGCAGAAGC CACAGAAGAG 
GTGTCCTTGG ACAGTCCCGA AAGAGAGCTC ATCCTGTCCT CCGAGCCGTC TCCTGCGGTC 
ACCCCTGTCA CTCCCACCAC ACTCATTGCT CCTAGAATCG AATCAAAGAG TATCTCTGCT 
CCAGTCATCT TTGACAGATC CAGGGACGAG ATTGAAGAAG AAGCAAATGG AGATATTTTT 
GATATAGAAA TTGGTGTGTC AGATCCGGAA AAAGTTGGTG ATGGCATGAA TGCTTACATG 
GCATATAGAG TAACAACAAA GACTTCTCTT TCCATGTTCA GTAAGAGTGA ATTTTCAGTC 
AAAAGAAGAT TCAGTGACTT TCTTGGTTTG CATAGCAAAT TAGCAAGCAA ATATTTACAT 
GTTGGTTACA TCGTGCCACC CGCTCCAGAA AAGAGTATAG TAGGTATGAC CAAGGTCAAA 
GTAGGTAAAG AAGATTCATC ATCCACTGAG TTTGTAGAAA AACGAAGAGC GGCACTGGAG 
AGGTATCTTC AGAGAACAGT AAAGCATCCG ACATTGCTTC AGGATCCTGA CTTAAGGCAG 
TTTTTGGAAA GCTCAGAGCT GCCGAGAGCA GTTAATACGC AGGCTCTGAG TGGAGCAGGA 
ATATTGAGGA TGGTGAACAA GGCTGCCGAC GCTGTCAACA AAATGACAAT CAAGATGAAT 
GAATCGGATG CATGGTTTGA AGAAAAGCAA CAGCAATTTG AGAATCTTGA CCAGCAACTT 
CGGAAACTTC ATGCCAGTGT TGAAGCCTTG GTCTGTCATA GAAAAGAACT TTCAGCCAAC 
ACAGCCGCCT TTGCTAAGAG TGCTGCCATG CTAGGTAATT CTGAGGACCA CACTGCGCTC 
TCTAGAGCTT TGTCTCAGCT GGCAGAGGTT GAGGAGAAGA TAGACCAGTT ACATCAAGAG 
CAAGCTTTTG CTGACTTTTA CATGTTCTCA GAACTTCTTA GTGACTACAT TCGTCTCATT 
GCTGCAGTGA AAGGTGTGTT TGACCATCGG ATGAAGTGCT GGCAGAAATG GGAAGATGCT 
CAAATTACCT TGCTCAAAAA ACGTGAAACT GAGGCAAAAA TGATGGTTGC TAACAAGCCA 
GATAAAATTC AACAAGCTAA AAACGAGATA AGAGAGTGGG AGGCGAAAGT ACAACAAGGA 
GAGAGAGATT TTGAGCAGAT CTCTAAAACA ATTCGAAAAG AAGTGGGAAG ATTTGAGAAA 
GAACGAGTGA AAGATTTTAA AGCTGTTATC ATCAAGTACT TGGAGTCATT AGTACAAACA 
CAGCAACAGC TGATAAAGTA CTGGGAGGCA TTCCTACCTG AAGCCAAAGC CATTGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Snx2 ( NM_001357463.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001344392.1 Mus musculus sorting nexin 2 (Snx2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu70192
Clone ID Related Accession (Same CDS sequence)	XM_006526190.3 , NM_001357463.1
Accession Version	NM_001357463.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1572bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	sorting nexin-2 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC127421.3 and AC148020.3. On Oct 28, 2017 this sequence version replaced XM_006526190.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660817.152984.1, SRR1660815.172885.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGCCG AGAGGGAGCC TCCTCCGCTG GGGGACGTGA AGCCCACAGA CTTTGAGGAG 
CTGGAGGACG GAGAGGACCT GTTCACCAGC ACCGTCTCCA CCCTTGAGTC AAGTCCATCA 
TCTCCCGAAC CAGCCAGTCT CCCTGCAGAA GATATCAGTG CAAACTCTAA TGGCTCCAAA 
CCTGTAGAAG TTGTCCTAGA CGATGACAGA GAAGATCTTT TCGCAGAAGC CACAGAAGAG 
GTGTCCTTGG ACAGTCCCGA AAGAGAGCTC ATCCTGTCCT CCGAGCCGTC TCCTGCGGTC 
ACCCCTGTCA CTCCCACCAC ACTCATTGCT CCTAGAATCG AATCAAAGAG TATCTCTGCT 
CCAGTCATCT TTGACAGATC CAGGGACGAG ATTGAAGAAG AAGCAAATGG AGATATTTTT 
GATATAGAAA TTGGTGTGTC AGATCCGGAA AAAGTTGGTG ATGGCATGAA TGCTTACATG 
GCATATAGAG TAACAACAAA GACTTCTCTT TCCATGTTCA GTAAGAGTGA ATTTTCAGTC 
AAAAGAAGAT TCAGTGACTT TCTTGGTTTG CATAGCAAAT TAGCAAGCAA ATATTTACAT 
GTTGGTTACA TCGTGCCACC CGCTCCAGAA AAGAGTATAG TAGGTATGAC CAAGGTCAAA 
GTAGGTAAAG AAGATTCATC ATCCACTGAG TTTGTAGAAA AACGAAGAGC GGCACTGGAG 
AGGTATCTTC AGAGAACAGT AAAGCATCCG ACATTGCTTC AGGATCCTGA CTTAAGGCAG 
TTTTTGGAAA GCTCAGAGCT GCCGAGAGCA GTTAATACGC AGGCTCTGAG TGGAGCAGGA 
ATATTGAGGA TGGTGAACAA GGCTGCCGAC GCTGTCAACA AAATGACAAT CAAGATGAAT 
GAATCGGATG CATGGTTTGA AGAAAAGCAA CAGCAATTTG AGAATCTTGA CCAGCAACTT 
CGGAAACTTC ATGCCAGTGT TGAAGCCTTG GTCTGTCATA GAAAAGAACT TTCAGCCAAC 
ACAGCCGCCT TTGCTAAGAG TGCTGCCATG CTAGGTAATT CTGAGGACCA CACTGCGCTC 
TCTAGAGCTT TGTCTCAGCT GGCAGAGGTT GAGGAGAAGA TAGACCAGTT ACATCAAGAG 
CAAGCTTTTG CTGACTTTTA CATGTTCTCA GAACTTCTTA GTGACTACAT TCGTCTCATT 
GCTGCAGTGA AAGGTGTGTT TGACCATCGG ATGAAGTGCT GGCAGAAATG GGAAGATGCT 
CAAATTACCT TGCTCAAAAA ACGTGAAACT GAGGCAAAAA TGATGGTTGC TAACAAGCCA 
GATAAAATTC AACAAGCTAA AAACGAGATA AGAGAGGAAA TTGAAGAGTG GGAGGCGAAA 
GTACAACAAG GAGAGAGAGA TTTTGAGCAG ATCTCTAAAA CAATTCGAAA AGAAGTGGGA 
AGATTTGAGA AAGAACGAGT GAAAGATTTT AAAGCTGTTA TCATCAAGTA CTTGGAGTCA 
TTAGTACAAA CACAGCAACA GCTGATAAAG TACTGGGAGG CATTCCTACC TGAAGCCAAA 
GCCATTGCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001344392.1
CDS	89..1660
Translation	MAAEREPPPLGDVKPTDFEELEDGEDLFTSTVSTLESSPSSPEPASLPAEDISANSNGSKPVEVVLDDDREDLFAEATEEVSLDSPERELILSSEPSPAVTPVTPTTLIAPRIESKSISAPVIFDRSRDEIEEEANGDIFDIEIGVSDPEKVGDGMNAYMAYRVTTKTSLSMFSKSEFSVKRRFSDFLGLHSKLASKYLHVGYIVPPAPEKSIVGMTKVKVGKEDSSSTEFVEKRRAALERYLQRTVKHPTLLQDPDLRQFLESSELPRAVNTQALSGAGILRMVNKAADAVNKMTIKMNESDAWFEEKQQQFENLDQQLRKLHASVEALVCHRKELSANTAAFAKSAAMLGNSEDHTALSRALSQLAEVEEKIDQLHQEQAFADFYMFSELLSDYIRLIAAVKGVFDHRMKCWQKWEDAQITLLKKRETEAKMMVANKPDKIQQAKNEIREEIEEWEAKVQQGERDFEQISKTIRKEVGRFEKERVKDFKAVIIKYLESLVQTQQQLIKYWEAFLPEAKAIA

Target ORF information:

RefSeq Version	NM_001357463.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sorting nexin 2 (Snx2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001357463.1

ORF Insert Sequence:

ATGGCGGCCG AGAGGGAGCC TCCTCCGCTG GGGGACGTGA AGCCCACAGA CTTTGAGGAG 
CTGGAGGACG GAGAGGACCT GTTCACCAGC ACCGTCTCCA CCCTTGAGTC AAGTCCATCA 
TCTCCCGAAC CAGCCAGTCT CCCTGCAGAA GATATCAGTG CAAACTCTAA TGGCTCCAAA 
CCTGTAGAAG TTGTCCTAGA CGATGACAGA GAAGATCTTT TCGCAGAAGC CACAGAAGAG 
GTGTCCTTGG ACAGTCCCGA AAGAGAGCTC ATCCTGTCCT CCGAGCCGTC TCCTGCGGTC 
ACCCCTGTCA CTCCCACCAC ACTCATTGCT CCTAGAATCG AATCAAAGAG TATCTCTGCT 
CCAGTCATCT TTGACAGATC CAGGGACGAG ATTGAAGAAG AAGCAAATGG AGATATTTTT 
GATATAGAAA TTGGTGTGTC AGATCCGGAA AAAGTTGGTG ATGGCATGAA TGCTTACATG 
GCATATAGAG TAACAACAAA GACTTCTCTT TCCATGTTCA GTAAGAGTGA ATTTTCAGTC 
AAAAGAAGAT TCAGTGACTT TCTTGGTTTG CATAGCAAAT TAGCAAGCAA ATATTTACAT 
GTTGGTTACA TCGTGCCACC CGCTCCAGAA AAGAGTATAG TAGGTATGAC CAAGGTCAAA 
GTAGGTAAAG AAGATTCATC ATCCACTGAG TTTGTAGAAA AACGAAGAGC GGCACTGGAG 
AGGTATCTTC AGAGAACAGT AAAGCATCCG ACATTGCTTC AGGATCCTGA CTTAAGGCAG 
TTTTTGGAAA GCTCAGAGCT GCCGAGAGCA GTTAATACGC AGGCTCTGAG TGGAGCAGGA 
ATATTGAGGA TGGTGAACAA GGCTGCCGAC GCTGTCAACA AAATGACAAT CAAGATGAAT 
GAATCGGATG CATGGTTTGA AGAAAAGCAA CAGCAATTTG AGAATCTTGA CCAGCAACTT 
CGGAAACTTC ATGCCAGTGT TGAAGCCTTG GTCTGTCATA GAAAAGAACT TTCAGCCAAC 
ACAGCCGCCT TTGCTAAGAG TGCTGCCATG CTAGGTAATT CTGAGGACCA CACTGCGCTC 
TCTAGAGCTT TGTCTCAGCT GGCAGAGGTT GAGGAGAAGA TAGACCAGTT ACATCAAGAG 
CAAGCTTTTG CTGACTTTTA CATGTTCTCA GAACTTCTTA GTGACTACAT TCGTCTCATT 
GCTGCAGTGA AAGGTGTGTT TGACCATCGG ATGAAGTGCT GGCAGAAATG GGAAGATGCT 
CAAATTACCT TGCTCAAAAA ACGTGAAACT GAGGCAAAAA TGATGGTTGC TAACAAGCCA 
GATAAAATTC AACAAGCTAA AAACGAGATA AGAGAGGAAA TTGAAGAGTG GGAGGCGAAA 
GTACAACAAG GAGAGAGAGA TTTTGAGCAG ATCTCTAAAA CAATTCGAAA AGAAGTGGGA 
AGATTTGAGA AAGAACGAGT GAAAGATTTT AAAGCTGTTA TCATCAAGTA CTTGGAGTCA 
TTAGTACAAA CACAGCAACA GCTGATAAAG TACTGGGAGG CATTCCTACC TGAAGCCAAA 
GCCATTGCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Snx2 ( XM_006526190.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006526253.2 Mus musculus sorting nexin 2 (Snx2), transcript variant X1, mRNA.

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CloneID	OMu70192
Clone ID Related Accession (Same CDS sequence)	XM_006526190.3 , NM_001357463.1
Accession Version	XM_006526190.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1572bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	sorting nexin-2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006526190.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCG AGAGGGAGCC TCCTCCGCTG GGGGACGTGA AGCCCACAGA CTTTGAGGAG 
CTGGAGGACG GAGAGGACCT GTTCACCAGC ACCGTCTCCA CCCTTGAGTC AAGTCCATCA 
TCTCCCGAAC CAGCCAGTCT CCCTGCAGAA GATATCAGTG CAAACTCTAA TGGCTCCAAA 
CCTGTAGAAG TTGTCCTAGA CGATGACAGA GAAGATCTTT TCGCAGAAGC CACAGAAGAG 
GTGTCCTTGG ACAGTCCCGA AAGAGAGCTC ATCCTGTCCT CCGAGCCGTC TCCTGCGGTC 
ACCCCTGTCA CTCCCACCAC ACTCATTGCT CCTAGAATCG AATCAAAGAG TATCTCTGCT 
CCAGTCATCT TTGACAGATC CAGGGACGAG ATTGAAGAAG AAGCAAATGG AGATATTTTT 
GATATAGAAA TTGGTGTGTC AGATCCGGAA AAAGTTGGTG ATGGCATGAA TGCTTACATG 
GCATATAGAG TAACAACAAA GACTTCTCTT TCCATGTTCA GTAAGAGTGA ATTTTCAGTC 
AAAAGAAGAT TCAGTGACTT TCTTGGTTTG CATAGCAAAT TAGCAAGCAA ATATTTACAT 
GTTGGTTACA TCGTGCCACC CGCTCCAGAA AAGAGTATAG TAGGTATGAC CAAGGTCAAA 
GTAGGTAAAG AAGATTCATC ATCCACTGAG TTTGTAGAAA AACGAAGAGC GGCACTGGAG 
AGGTATCTTC AGAGAACAGT AAAGCATCCG ACATTGCTTC AGGATCCTGA CTTAAGGCAG 
TTTTTGGAAA GCTCAGAGCT GCCGAGAGCA GTTAATACGC AGGCTCTGAG TGGAGCAGGA 
ATATTGAGGA TGGTGAACAA GGCTGCCGAC GCTGTCAACA AAATGACAAT CAAGATGAAT 
GAATCGGATG CATGGTTTGA AGAAAAGCAA CAGCAATTTG AGAATCTTGA CCAGCAACTT 
CGGAAACTTC ATGCCAGTGT TGAAGCCTTG GTCTGTCATA GAAAAGAACT TTCAGCCAAC 
ACAGCCGCCT TTGCTAAGAG TGCTGCCATG CTAGGTAATT CTGAGGACCA CACTGCGCTC 
TCTAGAGCTT TGTCTCAGCT GGCAGAGGTT GAGGAGAAGA TAGACCAGTT ACATCAAGAG 
CAAGCTTTTG CTGACTTTTA CATGTTCTCA GAACTTCTTA GTGACTACAT TCGTCTCATT 
GCTGCAGTGA AAGGTGTGTT TGACCATCGG ATGAAGTGCT GGCAGAAATG GGAAGATGCT 
CAAATTACCT TGCTCAAAAA ACGTGAAACT GAGGCAAAAA TGATGGTTGC TAACAAGCCA 
GATAAAATTC AACAAGCTAA AAACGAGATA AGAGAGGAAA TTGAAGAGTG GGAGGCGAAA 
GTACAACAAG GAGAGAGAGA TTTTGAGCAG ATCTCTAAAA CAATTCGAAA AGAAGTGGGA 
AGATTTGAGA AAGAACGAGT GAAAGATTTT AAAGCTGTTA TCATCAAGTA CTTGGAGTCA 
TTAGTACAAA CACAGCAACA GCTGATAAAG TACTGGGAGG CATTCCTACC TGAAGCCAAA 
GCCATTGCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006526253.2
CDS	92..1663
Translation	MAAEREPPPLGDVKPTDFEELEDGEDLFTSTVSTLESSPSSPEPASLPAEDISANSNGSKPVEVVLDDDREDLFAEATEEVSLDSPERELILSSEPSPAVTPVTPTTLIAPRIESKSISAPVIFDRSRDEIEEEANGDIFDIEIGVSDPEKVGDGMNAYMAYRVTTKTSLSMFSKSEFSVKRRFSDFLGLHSKLASKYLHVGYIVPPAPEKSIVGMTKVKVGKEDSSSTEFVEKRRAALERYLQRTVKHPTLLQDPDLRQFLESSELPRAVNTQALSGAGILRMVNKAADAVNKMTIKMNESDAWFEEKQQQFENLDQQLRKLHASVEALVCHRKELSANTAAFAKSAAMLGNSEDHTALSRALSQLAEVEEKIDQLHQEQAFADFYMFSELLSDYIRLIAAVKGVFDHRMKCWQKWEDAQITLLKKRETEAKMMVANKPDKIQQAKNEIREEIEEWEAKVQQGERDFEQISKTIRKEVGRFEKERVKDFKAVIIKYLESLVQTQQQLIKYWEAFLPEAKAIA

Target ORF information:

RefSeq Version	XM_006526190.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus sorting nexin 2 (Snx2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006526190.3

ORF Insert Sequence:

ATGGCGGCCG AGAGGGAGCC TCCTCCGCTG GGGGACGTGA AGCCCACAGA CTTTGAGGAG 
CTGGAGGACG GAGAGGACCT GTTCACCAGC ACCGTCTCCA CCCTTGAGTC AAGTCCATCA 
TCTCCCGAAC CAGCCAGTCT CCCTGCAGAA GATATCAGTG CAAACTCTAA TGGCTCCAAA 
CCTGTAGAAG TTGTCCTAGA CGATGACAGA GAAGATCTTT TCGCAGAAGC CACAGAAGAG 
GTGTCCTTGG ACAGTCCCGA AAGAGAGCTC ATCCTGTCCT CCGAGCCGTC TCCTGCGGTC 
ACCCCTGTCA CTCCCACCAC ACTCATTGCT CCTAGAATCG AATCAAAGAG TATCTCTGCT 
CCAGTCATCT TTGACAGATC CAGGGACGAG ATTGAAGAAG AAGCAAATGG AGATATTTTT 
GATATAGAAA TTGGTGTGTC AGATCCGGAA AAAGTTGGTG ATGGCATGAA TGCTTACATG 
GCATATAGAG TAACAACAAA GACTTCTCTT TCCATGTTCA GTAAGAGTGA ATTTTCAGTC 
AAAAGAAGAT TCAGTGACTT TCTTGGTTTG CATAGCAAAT TAGCAAGCAA ATATTTACAT 
GTTGGTTACA TCGTGCCACC CGCTCCAGAA AAGAGTATAG TAGGTATGAC CAAGGTCAAA 
GTAGGTAAAG AAGATTCATC ATCCACTGAG TTTGTAGAAA AACGAAGAGC GGCACTGGAG 
AGGTATCTTC AGAGAACAGT AAAGCATCCG ACATTGCTTC AGGATCCTGA CTTAAGGCAG 
TTTTTGGAAA GCTCAGAGCT GCCGAGAGCA GTTAATACGC AGGCTCTGAG TGGAGCAGGA 
ATATTGAGGA TGGTGAACAA GGCTGCCGAC GCTGTCAACA AAATGACAAT CAAGATGAAT 
GAATCGGATG CATGGTTTGA AGAAAAGCAA CAGCAATTTG AGAATCTTGA CCAGCAACTT 
CGGAAACTTC ATGCCAGTGT TGAAGCCTTG GTCTGTCATA GAAAAGAACT TTCAGCCAAC 
ACAGCCGCCT TTGCTAAGAG TGCTGCCATG CTAGGTAATT CTGAGGACCA CACTGCGCTC 
TCTAGAGCTT TGTCTCAGCT GGCAGAGGTT GAGGAGAAGA TAGACCAGTT ACATCAAGAG 
CAAGCTTTTG CTGACTTTTA CATGTTCTCA GAACTTCTTA GTGACTACAT TCGTCTCATT 
GCTGCAGTGA AAGGTGTGTT TGACCATCGG ATGAAGTGCT GGCAGAAATG GGAAGATGCT 
CAAATTACCT TGCTCAAAAA ACGTGAAACT GAGGCAAAAA TGATGGTTGC TAACAAGCCA 
GATAAAATTC AACAAGCTAA AAACGAGATA AGAGAGGAAA TTGAAGAGTG GGAGGCGAAA 
GTACAACAAG GAGAGAGAGA TTTTGAGCAG ATCTCTAAAA CAATTCGAAA AGAAGTGGGA 
AGATTTGAGA AAGAACGAGT GAAAGATTTT AAAGCTGTTA TCATCAAGTA CTTGGAGTCA 
TTAGTACAAA CACAGCAACA GCTGATAAAG TACTGGGAGG CATTCCTACC TGAAGCCAAA 
GCCATTGCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Snx2 ( XM_030250577.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106437.1 Mus musculus sorting nexin 2 (Snx2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu87425
Clone ID Related Accession (Same CDS sequence)	XM_030250577.1
Accession Version	XM_030250577.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1416bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	sorting nexin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTTCG TGTTTCTCGA ATTGCCATAT GCTGTGATTT CCTTGATTTC CTTGTTTAAT 
TCACGTCTAG AAGCCACAGA AGAGGTGTCC TTGGACAGTC CCGAAAGAGA GCTCATCCTG 
TCCTCCGAGC CGTCTCCTGC GGTCACCCCT GTCACTCCCA CCACACTCAT TGCTCCTAGA 
ATCGAATCAA AGAGTATCTC TGCTCCAGTC ATCTTTGACA GATCCAGGGA CGAGATTGAA 
GAAGAAGCAA ATGGAGATAT TTTTGATATA GAAATTGGTG TGTCAGATCC GGAAAAAGTT 
GGTGATGGCA TGAATGCTTA CATGGCATAT AGAGTAACAA CAAAGACTTC TCTTTCCATG 
TTCAGTAAGA GTGAATTTTC AGTCAAAAGA AGATTCAGTG ACTTTCTTGG TTTGCATAGC 
AAATTAGCAA GCAAATATTT ACATGTTGGT TACATCGTGC CACCCGCTCC AGAAAAGAGT 
ATAGTAGGTA TGACCAAGGT CAAAGTAGGT AAAGAAGATT CATCATCCAC TGAGTTTGTA 
GAAAAACGAA GAGCGGCACT GGAGAGGTAT CTTCAGAGAA CAGTAAAGCA TCCGACATTG 
CTTCAGGATC CTGACTTAAG GCAGTTTTTG GAAAGCTCAG AGCTGCCGAG AGCAGTTAAT 
ACGCAGGCTC TGAGTGGAGC AGGAATATTG AGGATGGTGA ACAAGGCTGC CGACGCTGTC 
AACAAAATGA CAATCAAGAT GAATGAATCG GATGCATGGT TTGAAGAAAA GCAACAGCAA 
TTTGAGAATC TTGACCAGCA ACTTCGGAAA CTTCATGCCA GTGTTGAAGC CTTGGTCTGT 
CATAGAAAAG AACTTTCAGC CAACACAGCC GCCTTTGCTA AGAGTGCTGC CATGCTAGGT 
AATTCTGAGG ACCACACTGC GCTCTCTAGA GCTTTGTCTC AGCTGGCAGA GGTTGAGGAG 
AAGATAGACC AGTTACATCA AGAGCAAGCT TTTGCTGACT TTTACATGTT CTCAGAACTT 
CTTAGTGACT ACATTCGTCT CATTGCTGCA GTGAAAGGTG TGTTTGACCA TCGGATGAAG 
TGCTGGCAGA AATGGGAAGA TGCTCAAATT ACCTTGCTCA AAAAACGTGA AACTGAGGCA 
AAAATGATGG TTGCTAACAA GCCAGATAAA ATTCAACAAG CTAAAAACGA GATAAGAGAG 
GAAATTGAAG AGTGGGAGGC GAAAGTACAA CAAGGAGAGA GAGATTTTGA GCAGATCTCT 
AAAACAATTC GAAAAGAAGT GGGAAGATTT GAGAAAGAAC GAGTGAAAGA TTTTAAAGCT 
GTTATCATCA AGTACTTGGA GTCATTAGTA CAAACACAGC AACAGCTGAT AAAGTACTGG 
GAGGCATTCC TACCTGAAGC CAAAGCCATT GCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106437.1
CDS	897..2312
Translation	MSFVFLELPYAVISLISLFNSRLEATEEVSLDSPERELILSSEPSPAVTPVTPTTLIAPRIESKSISAPVIFDRSRDEIEEEANGDIFDIEIGVSDPEKVGDGMNAYMAYRVTTKTSLSMFSKSEFSVKRRFSDFLGLHSKLASKYLHVGYIVPPAPEKSIVGMTKVKVGKEDSSSTEFVEKRRAALERYLQRTVKHPTLLQDPDLRQFLESSELPRAVNTQALSGAGILRMVNKAADAVNKMTIKMNESDAWFEEKQQQFENLDQQLRKLHASVEALVCHRKELSANTAAFAKSAAMLGNSEDHTALSRALSQLAEVEEKIDQLHQEQAFADFYMFSELLSDYIRLIAAVKGVFDHRMKCWQKWEDAQITLLKKRETEAKMMVANKPDKIQQAKNEIREEIEEWEAKVQQGERDFEQISKTIRKEVGRFEKERVKDFKAVIIKYLESLVQTQQQLIKYWEAFLPEAKAIA

Target ORF information:

RefSeq Version	XM_030250577.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sorting nexin 2 (Snx2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250577.1

ORF Insert Sequence:

ATGTCTTTCG TGTTTCTCGA ATTGCCATAT GCTGTGATTT CCTTGATTTC CTTGTTTAAT 
TCACGTCTAG AAGCCACAGA AGAGGTGTCC TTGGACAGTC CCGAAAGAGA GCTCATCCTG 
TCCTCCGAGC CGTCTCCTGC GGTCACCCCT GTCACTCCCA CCACACTCAT TGCTCCTAGA 
ATCGAATCAA AGAGTATCTC TGCTCCAGTC ATCTTTGACA GATCCAGGGA CGAGATTGAA 
GAAGAAGCAA ATGGAGATAT TTTTGATATA GAAATTGGTG TGTCAGATCC GGAAAAAGTT 
GGTGATGGCA TGAATGCTTA CATGGCATAT AGAGTAACAA CAAAGACTTC TCTTTCCATG 
TTCAGTAAGA GTGAATTTTC AGTCAAAAGA AGATTCAGTG ACTTTCTTGG TTTGCATAGC 
AAATTAGCAA GCAAATATTT ACATGTTGGT TACATCGTGC CACCCGCTCC AGAAAAGAGT 
ATAGTAGGTA TGACCAAGGT CAAAGTAGGT AAAGAAGATT CATCATCCAC TGAGTTTGTA 
GAAAAACGAA GAGCGGCACT GGAGAGGTAT CTTCAGAGAA CAGTAAAGCA TCCGACATTG 
CTTCAGGATC CTGACTTAAG GCAGTTTTTG GAAAGCTCAG AGCTGCCGAG AGCAGTTAAT 
ACGCAGGCTC TGAGTGGAGC AGGAATATTG AGGATGGTGA ACAAGGCTGC CGACGCTGTC 
AACAAAATGA CAATCAAGAT GAATGAATCG GATGCATGGT TTGAAGAAAA GCAACAGCAA 
TTTGAGAATC TTGACCAGCA ACTTCGGAAA CTTCATGCCA GTGTTGAAGC CTTGGTCTGT 
CATAGAAAAG AACTTTCAGC CAACACAGCC GCCTTTGCTA AGAGTGCTGC CATGCTAGGT 
AATTCTGAGG ACCACACTGC GCTCTCTAGA GCTTTGTCTC AGCTGGCAGA GGTTGAGGAG 
AAGATAGACC AGTTACATCA AGAGCAAGCT TTTGCTGACT TTTACATGTT CTCAGAACTT 
CTTAGTGACT ACATTCGTCT CATTGCTGCA GTGAAAGGTG TGTTTGACCA TCGGATGAAG 
TGCTGGCAGA AATGGGAAGA TGCTCAAATT ACCTTGCTCA AAAAACGTGA AACTGAGGCA 
AAAATGATGG TTGCTAACAA GCCAGATAAA ATTCAACAAG CTAAAAACGA GATAAGAGAG 
GAAATTGAAG AGTGGGAGGC GAAAGTACAA CAAGGAGAGA GAGATTTTGA GCAGATCTCT 
AAAACAATTC GAAAAGAAGT GGGAAGATTT GAGAAAGAAC GAGTGAAAGA TTTTAAAGCT 
GTTATCATCA AGTACTTGGA GTCATTAGTA CAAACACAGC AACAGCTGAT AAAGTACTGG 
GAGGCATTCC TACCTGAAGC CAAAGCCATT GCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Biogenesis of lysosome-related organelles complex-1 subunit 1 (BLOS1) interacts with sorting nexin 2 and the endosomal sorting complex required for transport-I (ESCRT-I) component TSG101 to mediate the sorting of epidermal growth factor receptor into endosomal compartments.
The Journal of biological chemistry289(42)29180-94(2014 Oct)
Zhang A,He X,Zhang L,Yang L,Woodman P,Li W

Visceral endoderm expression of Yin-Yang1 (YY1) is required for VEGFA maintenance and yolk sac development.
PloS one8(3)e58828(2013)
Rhee S,Guerrero-Zayas MI,Wallingford MC,Ortiz-Pineda P,Mager J,Tremblay KD

Loss of the PlagL2 transcription factor affects lacteal uptake of chylomicrons.
Cell metabolism6(5)406-13(2007 Nov)
Van Dyck F,Braem CV,Chen Z,Declercq J,Deckers R,Kim BM,Ito S,Wu MK,Cohen DE,Dewerchin M,Derua R,Waelkens E,Fiette L,Roebroek A,Schuit F,Van de Ven WJ,Shivdasani RA

Genetic evidence for a mammalian retromer complex containing sorting nexins 1 and 2.
Proceedings of the National Academy of Sciences of the United States of America102(42)15173-7(2005 Oct)
Griffin CT,Trejo J,Magnuson T

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Snx2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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