N6amt1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	N6amt1
Entrez Gene ID	67768
Full Name	N-6 adenine-specific DNA methyltransferase 1 (putative)
Synonyms	5830445C04Rik,Hemk2,Pred28
General protein information	Preferred Names N-6 adenine-specific DNA methyltransferase 1 (putative) Names hemK methyltransferase family member 2 M-N6DNA MTase A N6-DNA methyltransferase A
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 16 Map Location: 16\|16 C3.3

mRNA and Protein(s)

mRNA	Protein	Name
NM_026366.2	NP_080642.1	methyltransferase N6AMT1 isoform 1
NM_001159331.1	NP_001152803.1	methyltransferase N6AMT1 isoform 2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	LOC615200	NP_001076982.1
Rattus norvegicus (Norway rat)	N6amt1	NP_001178521.1
Caenorhabditis elegans (roundworm)	CELE_C33C12.9	NP_494209.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009458	XP_307877.2
Xenopus tropicalis (tropical clawed frog)	n6amt1	XP_004912981.1
Homo sapiens (human)	N6AMT1	NP_037372.3
Canis lupus familiaris (dog)	LOC487715	XP_544839.2
Schizosaccharomyces pombe (fission yeast)	SPAC323.05c	NP_594375.1
Pan troglodytes (chimpanzee)	N6AMT1	XP_003949193.1
Gallus gallus (chicken)	N6AMT1	NP_001265007.1
Drosophila melanogaster (fruit fly)	CG9960	NP_001027221.1
	N6AMT1	XP_001103046.1
Mus musculus (house mouse)	N6amt1	NP_080642.1
Danio rerio (zebrafish)	n6amt1	NP_001002502.1
Arabidopsis thaliana (thale cress)	AT3G13440	NP_187952.1
Oryza sativa (rice)	Os03g0824200	NP_001051749.1

Gene Ontology
Bibliography

Related articles in PubMed

Substrate Specificity of the HEMK2 Protein Glutamine Methyltransferase and Identification of Novel Substrates.
Kusevic D, Kudithipudi S, Jeltsch A
The Journal of biological chemistry291(12)6124-33(2016 Mar)

Deficiency in a glutamine-specific methyltransferase for release factor causes mouse embryonic lethality.
Liu P, Nie S, Li B, Yang ZQ, Xu ZM, Fei J, Lin C, Zeng R, Xu GL
Molecular and cellular biology30(17)4245-53(2010 Sep)

Cloning and primarily function study of two novel putative N5-glutamine methyltransferase (Hemk) splice variants from mouse stem cells.
Nie DS, Liu YB, Lu GX
Molecular biology reports36(8)2221-8(2009 Nov)

RNAi-mediated knock-down of gene mN6A1 reduces cell proliferation and decreases protein translation.
Liu Y, Nie D, Huang Y, Lu G
Molecular biology reports36(4)767-74(2009 Apr)

HemK2 protein, encoded on human chromosome 21, methylates translation termination factor eRF1.
Figaro S, Scrima N, Buckingham RH, Heurgu?-Hamard V
FEBS letters582(16)2352-6(2008 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

HEMK2 requires a GQX3R motif for methylation activity.
Title: Substrate Specificity of the HEMK2 Protein Glutamine Methyltransferase and Identification of Novel Substrates.

Disruption of N6amt1 gene led to early embryonic lethality.
Title: Deficiency in a glutamine-specific methyltransferase for release factor causes mouse embryonic lethality.

suggested that mN6A1 participates in protein translation through interaction with eRF1
Title: RNAi-mediated knock-down of gene mN6A1 reduces cell proliferation and decreases protein translation.

The murine PRED28 encodes two alternatively spliced RNA.
Title: Undetectable levels of N6-methyl adenine in mouse DNA: Cloning and analysis of PRED28, a gene coding for a putative mammalian DNA adenine methyltransferase.

The following N6amt1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the N6amt1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu02105	NM_026366.2 Latest version!	Mus musculus N-6 adenine-specific DNA methyltransferase 1 (putative) (N6amt1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
OMu07389	NM_001159331.1 Latest version!	Mus musculus N-6 adenine-specific DNA methyltransferase 1 (putative) (N6amt1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

N6amt1 ( NM_026366.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_080642.1 Mus musculus N-6 adenine-specific DNA methyltransferase 1 (putative) (N6amt1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu02105
Clone ID Related Accession (Same CDS sequence)	NM_026366.2
Accession Version	NM_026366.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 645bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	methyltransferase N6AMT1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AY456393.1 and AC140319.3. On Apr 14, 2009 this sequence version replaced NM_026366.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AY456393.1, SRR1660817.207926.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGCGC CGAGTGTCCC CACGCCGTTG TACGGGCACG TGGGTCGCGG AGCCTTCCGC 
GACGTGTACG AGCCAGCGGA GGACACGTTC CTGTTACTGG ACGCGCTCGA GGCGGCGGCG 
GCCGAGCTAG CAGGAGTGGA AATATGCCTT GAAGTAGGAG CAGGATCTGG TGTGGTGTCT 
GCATTCCTGG CCTCCATGAT AGGTCCTCGG GCCTTATACA TGTGCACTGA TATCAACCCT 
GAGGCAGCCG CATGTACCTT GGAAACAGCA CGCTGTAACA GAGTCCATGT TCAGCCAGTG 
ATCACAGATT TGGTGCACGG CTTGCTGCCC AGACTGAAGG GGAAAGTAGA CCTGCTGGTG 
TTTAACCCCC CCTATGTAGT GACTCCGCCT GAAGAGGTAG GAAGTCGTGG AATAGAAGCA 
GCCTGGGCTG GCGGCAGAAA CGGCCGGGAA GTCATGGACA GGTTCTTCCC ACTGGCTCCA 
GAACTCCTCT CCCCAAGAGG GCTGTTCTAC TTAGTTACCG TAAAAGAAAA CAATCCCGAG 
GAAATCTTTA AAACAATGAA GACAAGAGGT CTGCAAGGGA CCACAGCACT TTGCAGGCAA 
GCAGGCCAAG AAGCCCTGTC AGTCCTCAGG TTCAGCAAGT CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_080642.1
CDS	69..713
Translation	MAAPSVPTPLYGHVGRGAFRDVYEPAEDTFLLLDALEAAAAELAGVEICLEVGAGSGVVSAFLASMIGPRALYMCTDINPEAAACTLETARCNRVHVQPVITDLVHGLLPRLKGKVDLLVFNPPYVVTPPEEVGSRGIEAAWAGGRNGREVMDRFFPLAPELLSPRGLFYLVTVKENNPEEIFKTMKTRGLQGTTALCRQAGQEALSVLRFSKS

Target ORF information:

RefSeq Version	NM_026366.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus N-6 adenine-specific DNA methyltransferase 1 (putative) (N6amt1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_026366.2

ORF Insert Sequence:

ATGGCGGCGC CGAGTGTCCC CACGCCGTTG TACGGGCACG TGGGTCGCGG AGCCTTCCGC 
GACGTGTACG AGCCAGCGGA GGACACGTTC CTGTTACTGG ACGCGCTCGA GGCGGCGGCG 
GCCGAGCTAG CAGGAGTGGA AATATGCCTT GAAGTAGGAG CAGGATCTGG TGTGGTGTCT 
GCATTCCTGG CCTCCATGAT AGGTCCTCGG GCCTTATACA TGTGCACTGA TATCAACCCT 
GAGGCAGCCG CATGTACCTT GGAAACAGCA CGCTGTAACA GAGTCCATGT TCAGCCAGTG 
ATCACAGATT TGGTGCACGG CTTGCTGCCC AGACTGAAGG GGAAAGTAGA CCTGCTGGTG 
TTTAACCCCC CCTATGTAGT GACTCCGCCT GAAGAGGTAG GAAGTCGTGG AATAGAAGCA 
GCCTGGGCTG GCGGCAGAAA CGGCCGGGAA GTCATGGACA GGTTCTTCCC ACTGGCTCCA 
GAACTCCTCT CCCCAAGAGG GCTGTTCTAC TTAGTTACCG TAAAAGAAAA CAATCCCGAG 
GAAATCTTTA AAACAATGAA GACAAGAGGT CTGCAAGGGA CCACAGCACT TTGCAGGCAA 
GCAGGCCAAG AAGCCCTGTC AGTCCTCAGG TTCAGCAAGT CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

N6amt1 ( NM_001159331.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001152803.1 Mus musculus N-6 adenine-specific DNA methyltransferase 1 (putative) (N6amt1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu07389
Clone ID Related Accession (Same CDS sequence)	NM_001159331.1
Accession Version	NM_001159331.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	methyltransferase N6AMT1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AY456393.1 and AC140319.3. Transcript Variant: This variant (2) lacks an alternate coding exon compared to variant 1, that causes a frameshift. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AY583759.1, SRR1660813.215991.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849377 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGCGC CGAGTGTCCC CACGCCGTTG TACGGGCACG TGGGTCGCGG AGCCTTCCGC 
GACGTGTACG AGCCAGCGGA GGACACGTTC CTGTTACTGG ACGCGCTCGA GGCGGCGGCG 
GCCGAGCTAG CAGGAGTGGA AATATGCCTT GAAGTAGGAG CAGGATCTGG TGTGGTGTCT 
GCATTCCTGG CCTCCATGAT AGGTCCTCGG GCCTTATACA TGTGCACTGA TATCAACCCT 
GAGGCAGCCG CATGTACCTT GGAAACAGCA CGCTGTAACA GAGTCCATGT TCAGCCAGTG 
ATCACAGATT TGGTGCACGG CTTGCTGCCC AGACTGAAGG GGAAAGTAGA CCTGCTGGTG 
TTTAACCCCC CCTATGTAGT GACTCCGCCT GAAGAGAGGA AATCTTTAAA ACAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001152803.1
CDS	69..485
Translation	MAAPSVPTPLYGHVGRGAFRDVYEPAEDTFLLLDALEAAAAELAGVEICLEVGAGSGVVSAFLASMIGPRALYMCTDINPEAAACTLETARCNRVHVQPVITDLVHGLLPRLKGKVDLLVFNPPYVVTPPEERKSLKQ

Target ORF information:

RefSeq Version	NM_001159331.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus N-6 adenine-specific DNA methyltransferase 1 (putative) (N6amt1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001159331.1

ORF Insert Sequence:

ATGGCGGCGC CGAGTGTCCC CACGCCGTTG TACGGGCACG TGGGTCGCGG AGCCTTCCGC 
GACGTGTACG AGCCAGCGGA GGACACGTTC CTGTTACTGG ACGCGCTCGA GGCGGCGGCG 
GCCGAGCTAG CAGGAGTGGA AATATGCCTT GAAGTAGGAG CAGGATCTGG TGTGGTGTCT 
GCATTCCTGG CCTCCATGAT AGGTCCTCGG GCCTTATACA TGTGCACTGA TATCAACCCT 
GAGGCAGCCG CATGTACCTT GGAAACAGCA CGCTGTAACA GAGTCCATGT TCAGCCAGTG 
ATCACAGATT TGGTGCACGG CTTGCTGCCC AGACTGAAGG GGAAAGTAGA CCTGCTGGTG 
TTTAACCCCC CCTATGTAGT GACTCCGCCT GAAGAGAGGA AATCTTTAAA ACAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Substrate Specificity of the HEMK2 Protein Glutamine Methyltransferase and Identification of Novel Substrates.
The Journal of biological chemistry291(12)6124-33(2016 Mar)
Kusevic D,Kudithipudi S,Jeltsch A

Deficiency in a glutamine-specific methyltransferase for release factor causes mouse embryonic lethality.
Molecular and cellular biology30(17)4245-53(2010 Sep)
Liu P,Nie S,Li B,Yang ZQ,Xu ZM,Fei J,Lin C,Zeng R,Xu GL

Cloning and primarily function study of two novel putative N5-glutamine methyltransferase (Hemk) splice variants from mouse stem cells.
Molecular biology reports36(8)2221-8(2009 Nov)
Nie DS,Liu YB,Lu GX

RNAi-mediated knock-down of gene mN6A1 reduces cell proliferation and decreases protein translation.
Molecular biology reports36(4)767-74(2009 Apr)
Liu Y,Nie D,Huang Y,Lu G

HemK2 protein, encoded on human chromosome 21, methylates translation termination factor eRF1.
FEBS letters582(16)2352-6(2008 Jul)
Figaro S,Scrima N,Buckingham RH,Heurgu?-Hamard V