Eqtn cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Eqtn
Entrez Gene ID	67753
Full Name	equatorin, sperm acrosome associated
Synonyms	1700028B15Rik,4930579C15Rik,Afaf,C81058,Eqtl,Eqts
General protein information	Preferred Names equatorin, sperm acrosome associated Names equatorin MN9 antigen acrosome formation associated factor sperm-specific single transmembrane domain protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4\|4 C5

mRNA and Protein(s)

mRNA	Protein	Name
XM_011240602.1	XP_011238904.1	equatorin isoform X2
NM_001290623.1	NP_001277552.1	equatorin isoform 2 precursor
XM_006503333.4	XP_006503396.1	equatorin isoform X1
NM_027089.4	NP_081365.2	equatorin isoform 1 precursor
XM_006503333.3	XP_006503396.1	equatorin isoform X1
XM_011240601.2	XP_011238903.1	equatorin isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	EQTN	XP_528578.2
	EQTN	XP_001105128.1
Homo sapiens (human)	EQTN	NP_065692.2
Canis lupus familiaris (dog)	EQTN	XP_854434.1
Bos taurus (cattle)	EQTN	XP_002689540.1
Mus musculus (house mouse)	Eqtn	NP_081365.2
Rattus norvegicus (Norway rat)	Eqtn	NP_001034434.1

Gene Ontology
Bibliography

Related articles in PubMed

Equatorin is not essential for acrosome biogenesis but is required for the acrosome reaction.
Hao J, Chen M, Ji S, Wang X, Wang Y, Huang X, Yang L, Wang Y, Cui X, Lv L, Liu Y, Gao F
Biochemical and biophysical research communications444(4)537-42(2014 Feb)

Integration of the mouse sperm fertilization-related protein equatorin into the acrosome during spermatogenesis as revealed by super-resolution and immunoelectron microscopy.
Ito C, Yamatoya K, Yoshida K, Fujimura L, Hatano M, Miyado K, Toshimori K
Cell and tissue research352(3)739-50(2013 Jun)

A model of the acrosome reaction progression via the acrosomal membrane-anchored protein equatorin.
Yoshida K, Ito C, Yamatoya K, Maekawa M, Toyama Y, Suzuki-Toyota F, Toshimori K
Reproduction (Cambridge, England)139(3)533-44(2010 Mar)

Equatorin: identification and characterization of the epitope of the MN9 antibody in the mouse.
Yamatoya K, Yoshida K, Ito C, Maekawa M, Yanagida M, Takamori K, Ogawa H, Araki Y, Miyado K, Toyama Y, Toshimori K
Biology of reproduction81(5)889-97(2009 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The fertilization and induced acrosome exocytosis rates of Eqtn-deficient sperm were dramatically reduced.
Title: Equatorin is not essential for acrosome biogenesis but is required for the acrosome reaction.

The present study aims to clarify the morphology associated with the integration of the mouse fertilization-related acrosomal protein equatorin (mEQT) into the developing acrosome.
Title: Integration of the mouse sperm fertilization-related protein equatorin into the acrosome during spermatogenesis as revealed by super-resolution and immunoelectron microscopy.

Equatorin-based progression model will be useful for analyzing not only the behavior of equatorin but also of other molecules of interest involved in the acrosome reaction.
Title: A model of the acrosome reaction progression via the acrosomal membrane-anchored protein equatorin.

Acrosome formation-associated factor (Afaf) might play an important role in membrane trafficking during acrosome formation and participate in fertilization.
Title: Acrosome formation-associated factor is involved in fertilization.

Afaf expressed abundantly in the round spermatids, localized in the inner and outer membrane of forming acrosome, and declined in the maturing acrosomes.
Title: Afaf, a novel vesicle membrane protein, is related to acrosome formation in murine testis.

The following Eqtn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Eqtn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu33766	XM_011240602.1 Latest version!	Mus musculus equatorin, sperm acrosome associated (Eqtn), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu33766	XM_011240601.2 Latest version!	Mus musculus equatorin, sperm acrosome associated (Eqtn), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu20370	NM_001290623.1 Latest version!	Mus musculus equatorin, sperm acrosome associated (Eqtn), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu20334	NM_027089.4 Latest version!	Mus musculus equatorin, sperm acrosome associated (Eqtn), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
OMu33764	XM_006503333.4 Latest version!	Mus musculus equatorin, sperm acrosome associated (Eqtn), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OMu33764	XM_006503333.3	Mus musculus equatorin, sperm acrosome associated (Eqtn), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Eqtn ( XM_011240602.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011238904.1 Mus musculus equatorin, sperm acrosome associated (Eqtn), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu33766
Clone ID Related Accession (Same CDS sequence)	XM_011240602.1 , XM_011240601.2
Accession Version	XM_011240602.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	equatorin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGATA AAGATCAATT TTTTCAACCA ATTCCAGCCT CAGATTTGAA TGCTACCAAT 
GAAGACAAAC TGTCAGAGCT CGAGGAAATC AAGCTGAAGT TAATGCTGGG GATCTCGCTG 
ATGACACTGG TTCTGCTAAT CCCCCTCTTG ATATTCTGCT TTGCCACACT GTACAAACTG 
AGACACCTAC GTGACAAAAG TTATGAGAGT CAATACTCCA TCAACCCAGA GCTGGCAACT 
CTATCTTACT TCCATCCAAC AGAAGGTGTG TCAGATACAT CTTTTTCGAA AAGTGCAGAC 
AGCAACTCTT ATTGGGTCCA CAATTCTTCA GAGATGAGGC GATCAAGGAC AAGAAGGTCA 
AAATCTAAAC CTATGGATTT TTCTGCAGGC TCCAATCAAA CAGTCTTAAC CGACGAGTCA 
AGCTTCCTCC CTCCTGAGGA GACAAGATTC CTCCTTCCTG AGGAGCCGGG TAAGGAGCTC 
ATCGTTGAGA GAGGCCCTAT GCAGGCAATG AATGAGATTG ATGCCCAGCT GTTGTTGAAC 
AAGGAAGGAT CACCGAGTAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011238904.1
CDS	1353..1916
Translation	MDDKDQFFQPIPASDLNATNEDKLSELEEIKLKLMLGISLMTLVLLIPLLIFCFATLYKLRHLRDKSYESQYSINPELATLSYFHPTEGVSDTSFSKSADSNSYWVHNSSEMRRSRTRRSKSKPMDFSAGSNQTVLTDESSFLPPEETRFLLPEEPGKELIVERGPMQAMNEIDAQLLLNKEGSPSN

Target ORF information:

RefSeq Version	XM_011240602.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus equatorin, sperm acrosome associated (Eqtn), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011240602.1

ORF Insert Sequence:

ATGGATGATA AAGATCAATT TTTTCAACCA ATTCCAGCCT CAGATTTGAA TGCTACCAAT 
GAAGACAAAC TGTCAGAGCT CGAGGAAATC AAGCTGAAGT TAATGCTGGG GATCTCGCTG 
ATGACACTGG TTCTGCTAAT CCCCCTCTTG ATATTCTGCT TTGCCACACT GTACAAACTG 
AGACACCTAC GTGACAAAAG TTATGAGAGT CAATACTCCA TCAACCCAGA GCTGGCAACT 
CTATCTTACT TCCATCCAAC AGAAGGTGTG TCAGATACAT CTTTTTCGAA AAGTGCAGAC 
AGCAACTCTT ATTGGGTCCA CAATTCTTCA GAGATGAGGC GATCAAGGAC AAGAAGGTCA 
AAATCTAAAC CTATGGATTT TTCTGCAGGC TCCAATCAAA CAGTCTTAAC CGACGAGTCA 
AGCTTCCTCC CTCCTGAGGA GACAAGATTC CTCCTTCCTG AGGAGCCGGG TAAGGAGCTC 
ATCGTTGAGA GAGGCCCTAT GCAGGCAATG AATGAGATTG ATGCCCAGCT GTTGTTGAAC 
AAGGAAGGAT CACCGAGTAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eqtn ( XM_011240601.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011238903.1 Mus musculus equatorin, sperm acrosome associated (Eqtn), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu33766
Clone ID Related Accession (Same CDS sequence)	XM_011240602.1 , XM_011240601.2
Accession Version	XM_011240601.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	equatorin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011240601.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGATA AAGATCAATT TTTTCAACCA ATTCCAGCCT CAGATTTGAA TGCTACCAAT 
GAAGACAAAC TGTCAGAGCT CGAGGAAATC AAGCTGAAGT TAATGCTGGG GATCTCGCTG 
ATGACACTGG TTCTGCTAAT CCCCCTCTTG ATATTCTGCT TTGCCACACT GTACAAACTG 
AGACACCTAC GTGACAAAAG TTATGAGAGT CAATACTCCA TCAACCCAGA GCTGGCAACT 
CTATCTTACT TCCATCCAAC AGAAGGTGTG TCAGATACAT CTTTTTCGAA AAGTGCAGAC 
AGCAACTCTT ATTGGGTCCA CAATTCTTCA GAGATGAGGC GATCAAGGAC AAGAAGGTCA 
AAATCTAAAC CTATGGATTT TTCTGCAGGC TCCAATCAAA CAGTCTTAAC CGACGAGTCA 
AGCTTCCTCC CTCCTGAGGA GACAAGATTC CTCCTTCCTG AGGAGCCGGG TAAGGAGCTC 
ATCGTTGAGA GAGGCCCTAT GCAGGCAATG AATGAGATTG ATGCCCAGCT GTTGTTGAAC 
AAGGAAGGAT CACCGAGTAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011238903.1
CDS	1128..1691
Translation	MDDKDQFFQPIPASDLNATNEDKLSELEEIKLKLMLGISLMTLVLLIPLLIFCFATLYKLRHLRDKSYESQYSINPELATLSYFHPTEGVSDTSFSKSADSNSYWVHNSSEMRRSRTRRSKSKPMDFSAGSNQTVLTDESSFLPPEETRFLLPEEPGKELIVERGPMQAMNEIDAQLLLNKEGSPSN

Target ORF information:

RefSeq Version	XM_011240601.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus equatorin, sperm acrosome associated (Eqtn), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011240601.2

ORF Insert Sequence:

ATGGATGATA AAGATCAATT TTTTCAACCA ATTCCAGCCT CAGATTTGAA TGCTACCAAT 
GAAGACAAAC TGTCAGAGCT CGAGGAAATC AAGCTGAAGT TAATGCTGGG GATCTCGCTG 
ATGACACTGG TTCTGCTAAT CCCCCTCTTG ATATTCTGCT TTGCCACACT GTACAAACTG 
AGACACCTAC GTGACAAAAG TTATGAGAGT CAATACTCCA TCAACCCAGA GCTGGCAACT 
CTATCTTACT TCCATCCAAC AGAAGGTGTG TCAGATACAT CTTTTTCGAA AAGTGCAGAC 
AGCAACTCTT ATTGGGTCCA CAATTCTTCA GAGATGAGGC GATCAAGGAC AAGAAGGTCA 
AAATCTAAAC CTATGGATTT TTCTGCAGGC TCCAATCAAA CAGTCTTAAC CGACGAGTCA 
AGCTTCCTCC CTCCTGAGGA GACAAGATTC CTCCTTCCTG AGGAGCCGGG TAAGGAGCTC 
ATCGTTGAGA GAGGCCCTAT GCAGGCAATG AATGAGATTG ATGCCCAGCT GTTGTTGAAC 
AAGGAAGGAT CACCGAGTAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eqtn ( NM_001290623.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001277552.1 Mus musculus equatorin, sperm acrosome associated (Eqtn), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu20370
Clone ID Related Accession (Same CDS sequence)	NM_001290623.1
Accession Version	NM_001290623.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 891bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Mus musculus(house mouse)
Product	equatorin isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB572592.1, BY716419.1, AK016738.1, BE632290.1, AK016313.1 and BB018628.1. On Mar 15, 2014 this sequence version replaced XM_006503334.1. Transcript Variant: This variant (2) uses alternate in-frame splice sites in the 3' coding region, compared to variant 1. It encodes isoform 2, which lacks an internal segment and is shorter, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK016313.1, AK016738.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849384 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATTTTA TACTGTTGAT CATTTTATCT GGGGTTTTCC TCCCAGACAT TATCAGTCTA 
CAGCCCATTG TGGGACAGGA GCCTGGTGTG ACACTTTCAG ATGAAGAACA GTATTATGCA 
GATGAAGAAA ATAATACAGA TGGGAATTCT GTTGCTTTAC ATAAATTGGA AGAGAATGAA 
ATGGACACTC CTGCCAATGA GAAAACTGGC AATTATTATA AAGACATAAA ACAATATGTG 
TTCACCACAC CAAACATAAA AGGTTCTGAA GTATCTGTGA CTGCCACAAC TAACCTGGAA 
TTTGCTGTGA AGAAGAACTA TAAAGCCAGC AAACCAACTG CCAGTGGAGA AGAAGAAAAA 
CCTAGTGAAT CCTCTCGTAA AACCTCAACC CCCAATATAC CTGCATTTTG GACAATATTA 
TCTAAAGCTG TAAATGAAAC AGCAGTGAGC ATGGATGATA AAGATCAATT TTTTCAACCA 
ATTCCAGCCT CAGATTTGAA TGCTACCAAT GAAGACAAAC TGTCAGAGCT CGAGGAAATC 
AAGCTGAAGT TAATGCTGGG GATCTCGCTG ATGACACTGG TTCTGCTAAT CCCCCTCTTG 
ATATTCTGCT TTGCCACACT GTACAAACTG AGACACCTAC GTGACAAAAG TTATGAGAGT 
CAATACTCCA TCAACCCAGA GCTGGCAACT CTATCTTACT TCCATCCAAC AGAAGGCTCC 
AATCAAACAG TCTTAACCGA CGAGTCAAGC TTCCTCCCTC CTGAGGAGAC AAGATTCCTC 
CTTCCTGAGG AGCCGGGTAA GGAGCTCATC GTTGAGAGAG GCCCTATGCA GGCAATGAAT 
GAGATTGATG CCCAGCTGTT GTTGAACAAG GAAGGATCAC CGAGTAATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001277552.1
CDS	92..982
Translation	MDFILLIILSGVFLPDIISLQPIVGQEPGVTLSDEEQYYADEENNTDGNSVALHKLEENEMDTPANEKTGNYYKDIKQYVFTTPNIKGSEVSVTATTNLEFAVKKNYKASKPTASGEEEKPSESSRKTSTPNIPAFWTILSKAVNETAVSMDDKDQFFQPIPASDLNATNEDKLSELEEIKLKLMLGISLMTLVLLIPLLIFCFATLYKLRHLRDKSYESQYSINPELATLSYFHPTEGSNQTVLTDESSFLPPEETRFLLPEEPGKELIVERGPMQAMNEIDAQLLLNKEGSPSN

Target ORF information:

RefSeq Version	NM_001290623.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus equatorin, sperm acrosome associated (Eqtn), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001290623.1

ORF Insert Sequence:

ATGGATTTTA TACTGTTGAT CATTTTATCT GGGGTTTTCC TCCCAGACAT TATCAGTCTA 
CAGCCCATTG TGGGACAGGA GCCTGGTGTG ACACTTTCAG ATGAAGAACA GTATTATGCA 
GATGAAGAAA ATAATACAGA TGGGAATTCT GTTGCTTTAC ATAAATTGGA AGAGAATGAA 
ATGGACACTC CTGCCAATGA GAAAACTGGC AATTATTATA AAGACATAAA ACAATATGTG 
TTCACCACAC CAAACATAAA AGGTTCTGAA GTATCTGTGA CTGCCACAAC TAACCTGGAA 
TTTGCTGTGA AGAAGAACTA TAAAGCCAGC AAACCAACTG CCAGTGGAGA AGAAGAAAAA 
CCTAGTGAAT CCTCTCGTAA AACCTCAACC CCCAATATAC CTGCATTTTG GACAATATTA 
TCTAAAGCTG TAAATGAAAC AGCAGTGAGC ATGGATGATA AAGATCAATT TTTTCAACCA 
ATTCCAGCCT CAGATTTGAA TGCTACCAAT GAAGACAAAC TGTCAGAGCT CGAGGAAATC 
AAGCTGAAGT TAATGCTGGG GATCTCGCTG ATGACACTGG TTCTGCTAAT CCCCCTCTTG 
ATATTCTGCT TTGCCACACT GTACAAACTG AGACACCTAC GTGACAAAAG TTATGAGAGT 
CAATACTCCA TCAACCCAGA GCTGGCAACT CTATCTTACT TCCATCCAAC AGAAGGCTCC 
AATCAAACAG TCTTAACCGA CGAGTCAAGC TTCCTCCCTC CTGAGGAGAC AAGATTCCTC 
CTTCCTGAGG AGCCGGGTAA GGAGCTCATC GTTGAGAGAG GCCCTATGCA GGCAATGAAT 
GAGATTGATG CCCAGCTGTT GTTGAACAAG GAAGGATCAC CGAGTAATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eqtn ( XM_006503333.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006503396.1 Mus musculus equatorin, sperm acrosome associated (Eqtn), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu33764
Clone ID Related Accession (Same CDS sequence)	XM_006503333.4 , XM_006503333.3
Accession Version	XM_006503333.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	equatorin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006503333.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTTA TACTGTTGAT CATTTTATCT GGGGTTTTCC TCCCAGACAT TATCAGTCTA 
CAGCCCATTG TGGGACAGGA GCCTGGTGTG ACACTTTCAG ATGAAGAACA GTATTATGCA 
GATGAAGAAA ATAATACAGA TGGGAATTCT GTTGCTTTAC ATAAATTGGA AGAGAATGAA 
ATGGACACTC CTGCCAATGA GAAAACTGGC AATTATTATA AAGACATAAA ACAATATGTG 
TTCACCACAC CAAACATAAA AGGTTCTGAA GTATCTGTGA CTGCCACAAC TAACCTGGAA 
TTTGCTGTGA AGAAGAACTA TAAAGCCAGC AAACCAACTG CCAGTGGAGA AGAAGAAAAA 
CCTAGTGAAT CCTCTCGTAA AACCTCAACC CCCAATATAC CTGCATTTTG GACAATATTA 
TCTAAAGCTG TAAATGAAAC AGCAGTGAGC ATGGATGATA AAGATCAATT TTTTCAACCA 
ATTCCAGCCT CAGATTTGAA TGCTACCAAT GAAGACAAAC TGTCAGAGCT CGAGGAAATC 
AAGCTGAAGT TAATGCTGGG GATCTCGCTG ATGACACTGG TTCTGCTAAT CCCCCTCTTG 
ATATTCTGCT TTGCCACACT GTACAAACTG AGACACCTAC GTGACAAAAG TTATGAGAGT 
CAATACTCCA TCAACCCAGA GCTGGCAACT CTATCTTACT TCCATCCAAC AGAAGGTGTG 
TCAGATACAT CTTTTTCGAA AAGTGCAGAC AGCAACTCTT ATTGGGTCCA CAATTCTTCA 
GAGATGAGGC GATCAAGGAC AAGAAGGTCA AAATCTAAAC CTATGGATTT TTCTGCAGGC 
TCCAATCAAA CAGTCTTAAC CGACGAGTCA AGCTTCCTCC CTCCTGAGGA GACAAGATTC 
CTCCTTCCTG AGGAGCCGGC TGTAGCTGAC GGGGTCCAAT TGGAGGTCTG CTCCTCTGAG 
GCAAGACAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006503396.1
CDS	1201..2172
Translation	MDFILLIILSGVFLPDIISLQPIVGQEPGVTLSDEEQYYADEENNTDGNSVALHKLEENEMDTPANEKTGNYYKDIKQYVFTTPNIKGSEVSVTATTNLEFAVKKNYKASKPTASGEEEKPSESSRKTSTPNIPAFWTILSKAVNETAVSMDDKDQFFQPIPASDLNATNEDKLSELEEIKLKLMLGISLMTLVLLIPLLIFCFATLYKLRHLRDKSYESQYSINPELATLSYFHPTEGVSDTSFSKSADSNSYWVHNSSEMRRSRTRRSKSKPMDFSAGSNQTVLTDESSFLPPEETRFLLPEEPAVADGVQLEVCSSEARQ

Target ORF information:

RefSeq Version	XM_006503333.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus equatorin, sperm acrosome associated (Eqtn), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006503333.3

ORF Insert Sequence:

ATGGATTTTA TACTGTTGAT CATTTTATCT GGGGTTTTCC TCCCAGACAT TATCAGTCTA 
CAGCCCATTG TGGGACAGGA GCCTGGTGTG ACACTTTCAG ATGAAGAACA GTATTATGCA 
GATGAAGAAA ATAATACAGA TGGGAATTCT GTTGCTTTAC ATAAATTGGA AGAGAATGAA 
ATGGACACTC CTGCCAATGA GAAAACTGGC AATTATTATA AAGACATAAA ACAATATGTG 
TTCACCACAC CAAACATAAA AGGTTCTGAA GTATCTGTGA CTGCCACAAC TAACCTGGAA 
TTTGCTGTGA AGAAGAACTA TAAAGCCAGC AAACCAACTG CCAGTGGAGA AGAAGAAAAA 
CCTAGTGAAT CCTCTCGTAA AACCTCAACC CCCAATATAC CTGCATTTTG GACAATATTA 
TCTAAAGCTG TAAATGAAAC AGCAGTGAGC ATGGATGATA AAGATCAATT TTTTCAACCA 
ATTCCAGCCT CAGATTTGAA TGCTACCAAT GAAGACAAAC TGTCAGAGCT CGAGGAAATC 
AAGCTGAAGT TAATGCTGGG GATCTCGCTG ATGACACTGG TTCTGCTAAT CCCCCTCTTG 
ATATTCTGCT TTGCCACACT GTACAAACTG AGACACCTAC GTGACAAAAG TTATGAGAGT 
CAATACTCCA TCAACCCAGA GCTGGCAACT CTATCTTACT TCCATCCAAC AGAAGGTGTG 
TCAGATACAT CTTTTTCGAA AAGTGCAGAC AGCAACTCTT ATTGGGTCCA CAATTCTTCA 
GAGATGAGGC GATCAAGGAC AAGAAGGTCA AAATCTAAAC CTATGGATTT TTCTGCAGGC 
TCCAATCAAA CAGTCTTAAC CGACGAGTCA AGCTTCCTCC CTCCTGAGGA GACAAGATTC 
CTCCTTCCTG AGGAGCCGGC TGTAGCTGAC GGGGTCCAAT TGGAGGTCTG CTCCTCTGAG 
GCAAGACAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eqtn ( NM_027089.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_081365.2 Mus musculus equatorin, sperm acrosome associated (Eqtn), transcript variant 1, mRNA.

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CloneID	OMu20334
Clone ID Related Accession (Same CDS sequence)	NM_027089.4
Accession Version	NM_027089.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1014bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Mus musculus(house mouse)
Product	equatorin isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB572592.1, AK006446.1, AK016738.1, BE632290.1, AK016313.1 and BB018628.1. On Mar 15, 2014 this sequence version replaced NM_027089.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: BC089517.1, EU828781.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849384 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATTTTA TACTGTTGAT CATTTTATCT GGGGTTTTCC TCCCAGACAT TATCAGTCTA 
CAGCCCATTG TGGGACAGGA GCCTGGTGTG ACACTTTCAG ATGAAGAACA GTATTATGCA 
GATGAAGAAA ATAATACAGA TGGGAATTCT GTTGCTTTAC ATAAATTGGA AGAGAATGAA 
ATGGACACTC CTGCCAATGA GAAAACTGGC AATTATTATA AAGACATAAA ACAATATGTG 
TTCACCACAC CAAACATAAA AGGTTCTGAA GTATCTGTGA CTGCCACAAC TAACCTGGAA 
TTTGCTGTGA AGAAGAACTA TAAAGCCAGC AAACCAACTG CCAGTGGAGA AGAAGAAAAA 
CCTAGTGAAT CCTCTCGTAA AACCTCAACC CCCAATATAC CTGCATTTTG GACAATATTA 
TCTAAAGCTG TAAATGAAAC AGCAGTGAGC ATGGATGATA AAGATCAATT TTTTCAACCA 
ATTCCAGCCT CAGATTTGAA TGCTACCAAT GAAGACAAAC TGTCAGAGCT CGAGGAAATC 
AAGCTGAAGT TAATGCTGGG GATCTCGCTG ATGACACTGG TTCTGCTAAT CCCCCTCTTG 
ATATTCTGCT TTGCCACACT GTACAAACTG AGACACCTAC GTGACAAAAG TTATGAGAGT 
CAATACTCCA TCAACCCAGA GCTGGCAACT CTATCTTACT TCCATCCAAC AGAAGGTGTG 
TCAGATACAT CTTTTTCGAA AAGTGCAGAC AGCAACTCTT ATTGGGTCCA CAATTCTTCA 
GAGATGAGGC GATCAAGGAC AAGAAGGTCA AAATCTAAAC CTATGGATTT TTCTGCAGGC 
TCCAATCAAA CAGTCTTAAC CGACGAGTCA AGCTTCCTCC CTCCTGAGGA GACAAGATTC 
CTCCTTCCTG AGGAGCCGGG TAAGGAGCTC ATCGTTGAGA GAGGCCCTAT GCAGGCAATG 
AATGAGATTG ATGCCCAGCT GTTGTTGAAC AAGGAAGGAT CACCGAGTAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_081365.2
CDS	92..1105
Translation	MDFILLIILSGVFLPDIISLQPIVGQEPGVTLSDEEQYYADEENNTDGNSVALHKLEENEMDTPANEKTGNYYKDIKQYVFTTPNIKGSEVSVTATTNLEFAVKKNYKASKPTASGEEEKPSESSRKTSTPNIPAFWTILSKAVNETAVSMDDKDQFFQPIPASDLNATNEDKLSELEEIKLKLMLGISLMTLVLLIPLLIFCFATLYKLRHLRDKSYESQYSINPELATLSYFHPTEGVSDTSFSKSADSNSYWVHNSSEMRRSRTRRSKSKPMDFSAGSNQTVLTDESSFLPPEETRFLLPEEPGKELIVERGPMQAMNEIDAQLLLNKEGSPSN

Target ORF information:

RefSeq Version	NM_027089.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus equatorin, sperm acrosome associated (Eqtn), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_027089.4

ORF Insert Sequence:

ATGGATTTTA TACTGTTGAT CATTTTATCT GGGGTTTTCC TCCCAGACAT TATCAGTCTA 
CAGCCCATTG TGGGACAGGA GCCTGGTGTG ACACTTTCAG ATGAAGAACA GTATTATGCA 
GATGAAGAAA ATAATACAGA TGGGAATTCT GTTGCTTTAC ATAAATTGGA AGAGAATGAA 
ATGGACACTC CTGCCAATGA GAAAACTGGC AATTATTATA AAGACATAAA ACAATATGTG 
TTCACCACAC CAAACATAAA AGGTTCTGAA GTATCTGTGA CTGCCACAAC TAACCTGGAA 
TTTGCTGTGA AGAAGAACTA TAAAGCCAGC AAACCAACTG CCAGTGGAGA AGAAGAAAAA 
CCTAGTGAAT CCTCTCGTAA AACCTCAACC CCCAATATAC CTGCATTTTG GACAATATTA 
TCTAAAGCTG TAAATGAAAC AGCAGTGAGC ATGGATGATA AAGATCAATT TTTTCAACCA 
ATTCCAGCCT CAGATTTGAA TGCTACCAAT GAAGACAAAC TGTCAGAGCT CGAGGAAATC 
AAGCTGAAGT TAATGCTGGG GATCTCGCTG ATGACACTGG TTCTGCTAAT CCCCCTCTTG 
ATATTCTGCT TTGCCACACT GTACAAACTG AGACACCTAC GTGACAAAAG TTATGAGAGT 
CAATACTCCA TCAACCCAGA GCTGGCAACT CTATCTTACT TCCATCCAAC AGAAGGTGTG 
TCAGATACAT CTTTTTCGAA AAGTGCAGAC AGCAACTCTT ATTGGGTCCA CAATTCTTCA 
GAGATGAGGC GATCAAGGAC AAGAAGGTCA AAATCTAAAC CTATGGATTT TTCTGCAGGC 
TCCAATCAAA CAGTCTTAAC CGACGAGTCA AGCTTCCTCC CTCCTGAGGA GACAAGATTC 
CTCCTTCCTG AGGAGCCGGG TAAGGAGCTC ATCGTTGAGA GAGGCCCTAT GCAGGCAATG 
AATGAGATTG ATGCCCAGCT GTTGTTGAAC AAGGAAGGAT CACCGAGTAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eqtn ( XM_006503333.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006503396.1 Mus musculus equatorin, sperm acrosome associated (Eqtn), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu33764
Clone ID Related Accession (Same CDS sequence)	XM_006503333.4 , XM_006503333.3
Accession Version	XM_006503333.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	equatorin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006503333.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTTA TACTGTTGAT CATTTTATCT GGGGTTTTCC TCCCAGACAT TATCAGTCTA 
CAGCCCATTG TGGGACAGGA GCCTGGTGTG ACACTTTCAG ATGAAGAACA GTATTATGCA 
GATGAAGAAA ATAATACAGA TGGGAATTCT GTTGCTTTAC ATAAATTGGA AGAGAATGAA 
ATGGACACTC CTGCCAATGA GAAAACTGGC AATTATTATA AAGACATAAA ACAATATGTG 
TTCACCACAC CAAACATAAA AGGTTCTGAA GTATCTGTGA CTGCCACAAC TAACCTGGAA 
TTTGCTGTGA AGAAGAACTA TAAAGCCAGC AAACCAACTG CCAGTGGAGA AGAAGAAAAA 
CCTAGTGAAT CCTCTCGTAA AACCTCAACC CCCAATATAC CTGCATTTTG GACAATATTA 
TCTAAAGCTG TAAATGAAAC AGCAGTGAGC ATGGATGATA AAGATCAATT TTTTCAACCA 
ATTCCAGCCT CAGATTTGAA TGCTACCAAT GAAGACAAAC TGTCAGAGCT CGAGGAAATC 
AAGCTGAAGT TAATGCTGGG GATCTCGCTG ATGACACTGG TTCTGCTAAT CCCCCTCTTG 
ATATTCTGCT TTGCCACACT GTACAAACTG AGACACCTAC GTGACAAAAG TTATGAGAGT 
CAATACTCCA TCAACCCAGA GCTGGCAACT CTATCTTACT TCCATCCAAC AGAAGGTGTG 
TCAGATACAT CTTTTTCGAA AAGTGCAGAC AGCAACTCTT ATTGGGTCCA CAATTCTTCA 
GAGATGAGGC GATCAAGGAC AAGAAGGTCA AAATCTAAAC CTATGGATTT TTCTGCAGGC 
TCCAATCAAA CAGTCTTAAC CGACGAGTCA AGCTTCCTCC CTCCTGAGGA GACAAGATTC 
CTCCTTCCTG AGGAGCCGGC TGTAGCTGAC GGGGTCCAAT TGGAGGTCTG CTCCTCTGAG 
GCAAGACAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006503396.1
CDS	1202..2173
Translation	MDFILLIILSGVFLPDIISLQPIVGQEPGVTLSDEEQYYADEENNTDGNSVALHKLEENEMDTPANEKTGNYYKDIKQYVFTTPNIKGSEVSVTATTNLEFAVKKNYKASKPTASGEEEKPSESSRKTSTPNIPAFWTILSKAVNETAVSMDDKDQFFQPIPASDLNATNEDKLSELEEIKLKLMLGISLMTLVLLIPLLIFCFATLYKLRHLRDKSYESQYSINPELATLSYFHPTEGVSDTSFSKSADSNSYWVHNSSEMRRSRTRRSKSKPMDFSAGSNQTVLTDESSFLPPEETRFLLPEEPAVADGVQLEVCSSEARQ

Target ORF information:

RefSeq Version	XM_006503333.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus equatorin, sperm acrosome associated (Eqtn), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006503333.4

ORF Insert Sequence:

ATGGATTTTA TACTGTTGAT CATTTTATCT GGGGTTTTCC TCCCAGACAT TATCAGTCTA 
CAGCCCATTG TGGGACAGGA GCCTGGTGTG ACACTTTCAG ATGAAGAACA GTATTATGCA 
GATGAAGAAA ATAATACAGA TGGGAATTCT GTTGCTTTAC ATAAATTGGA AGAGAATGAA 
ATGGACACTC CTGCCAATGA GAAAACTGGC AATTATTATA AAGACATAAA ACAATATGTG 
TTCACCACAC CAAACATAAA AGGTTCTGAA GTATCTGTGA CTGCCACAAC TAACCTGGAA 
TTTGCTGTGA AGAAGAACTA TAAAGCCAGC AAACCAACTG CCAGTGGAGA AGAAGAAAAA 
CCTAGTGAAT CCTCTCGTAA AACCTCAACC CCCAATATAC CTGCATTTTG GACAATATTA 
TCTAAAGCTG TAAATGAAAC AGCAGTGAGC ATGGATGATA AAGATCAATT TTTTCAACCA 
ATTCCAGCCT CAGATTTGAA TGCTACCAAT GAAGACAAAC TGTCAGAGCT CGAGGAAATC 
AAGCTGAAGT TAATGCTGGG GATCTCGCTG ATGACACTGG TTCTGCTAAT CCCCCTCTTG 
ATATTCTGCT TTGCCACACT GTACAAACTG AGACACCTAC GTGACAAAAG TTATGAGAGT 
CAATACTCCA TCAACCCAGA GCTGGCAACT CTATCTTACT TCCATCCAAC AGAAGGTGTG 
TCAGATACAT CTTTTTCGAA AAGTGCAGAC AGCAACTCTT ATTGGGTCCA CAATTCTTCA 
GAGATGAGGC GATCAAGGAC AAGAAGGTCA AAATCTAAAC CTATGGATTT TTCTGCAGGC 
TCCAATCAAA CAGTCTTAAC CGACGAGTCA AGCTTCCTCC CTCCTGAGGA GACAAGATTC 
CTCCTTCCTG AGGAGCCGGC TGTAGCTGAC GGGGTCCAAT TGGAGGTCTG CTCCTCTGAG 
GCAAGACAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Equatorin is not essential for acrosome biogenesis but is required for the acrosome reaction.
Biochemical and biophysical research communications444(4)537-42(2014 Feb)
Hao J,Chen M,Ji S,Wang X,Wang Y,Huang X,Yang L,Wang Y,Cui X,Lv L,Liu Y,Gao F

Integration of the mouse sperm fertilization-related protein equatorin into the acrosome during spermatogenesis as revealed by super-resolution and immunoelectron microscopy.
Cell and tissue research352(3)739-50(2013 Jun)
Ito C,Yamatoya K,Yoshida K,Fujimura L,Hatano M,Miyado K,Toshimori K

A model of the acrosome reaction progression via the acrosomal membrane-anchored protein equatorin.
Reproduction (Cambridge, England)139(3)533-44(2010 Mar)
Yoshida K,Ito C,Yamatoya K,Maekawa M,Toyama Y,Suzuki-Toyota F,Toshimori K

Equatorin: identification and characterization of the epitope of the MN9 antibody in the mouse.
Biology of reproduction81(5)889-97(2009 Nov)
Yamatoya K,Yoshida K,Ito C,Maekawa M,Yanagida M,Takamori K,Ogawa H,Araki Y,Miyado K,Toyama Y,Toshimori K

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Eqtn cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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