Ift172 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ift172
Entrez Gene ID	67661
Full Name	intraflagellar transport 172
Synonyms	4930553F24Rik,Slb,avc1,wim
General protein information	Preferred Names intraflagellar transport 172 Names intraflagellar transport protein 172 homolog intraflagellar transport 172 homolog intraflagellar transport 172 protein protein wimple selective LIM-binding factor Wimple
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 B1

mRNA and Protein(s)

mRNA	Protein	Name
NM_026298.5	NP_080574.5	intraflagellar transport protein 172 homolog

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	IFT172	XP_419993.2
Drosophila melanogaster (fruit fly)	osm-1	NP_647700.2
	IFT172	XP_001097501.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005330	XP_555970.1
Bos taurus (cattle)	IFT172	XP_003586706.1
Xenopus tropicalis (tropical clawed frog)	LOC100490796	XP_002943869.2
Pan troglodytes (chimpanzee)	IFT172	XP_003308981.1
Mus musculus (house mouse)	Ift172	NP_080574.5
Rattus norvegicus (Norway rat)	Ift172	NP_446244.1
Homo sapiens (human)	IFT172	NP_056477.1
Canis lupus familiaris (dog)	IFT172	XP_540128.2
Danio rerio (zebrafish)	ift172	NP_001002312.1
Caenorhabditis elegans (roundworm)	osm-1	NP_510681.4

Gene Ontology
Bibliography

Related articles in PubMed

Proper ciliary assembly is critical for restricting Hedgehog signaling during early eye development in mice.
Burnett JB, Lupu FI, Eggenschwiler JT
Developmental biology430(1)32-40(2017 10)

The Meckel syndrome- associated protein MKS1 functionally interacts with components of the BBSome and IFT complexes to mediate ciliary trafficking and hedgehog signaling.
Goetz SC, Bangs F, Barrington CL, Katsanis N, Anderson KV
PloS one12(3)e0173399(2017)

Cilia gene mutations cause atrioventricular septal defects by multiple mechanisms.
Burnicka-Turek O, Steimle JD, Huang W, Felker L, Kamp A, Kweon J, Peterson M, Reeves RH, Maslen CL, Gruber PJ, Yang XH, Shendure J, Moskowitz IP
Human molecular genetics25(14)3011-3028(2016 07)

Does 3D Phenotyping Yield Substantial Insights in the Genetics of the Mouse Mandible Shape?
Navarro N, Maga AM
G3 (Bethesda, Md.)6(5)1153-63(2016 05)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mutation of Ift172 results in a specific class of abnormal cilia and resulting in the VACTERL-like features with hydrocephalus phenotype.
Title: A novel murine allele of Intraflagellar Transport Protein 172 causes a syndrome including VACTERL-like features with hydrocephalus.

Phenotype of the disrupted allele by using CRE expression directed by the prx1 enhancer to disrupt the conditional Ift172 allele in the developing limb.
Title: Generation of mice with a conditional allele for Ift172.

The formation and function of the node and anterior mesendoderm in the mouse embryo relies on an indispensable role of Ift172 in cilia morphogenesis and cilia-mediated signaling.
Title: Intraflagellar transport protein 172 is essential for primary cilia formation and plays a vital role in patterning the mammalian brain.

Ift172 is most abundantly expressed in tissues rich in highly ciliated cells, such as olfactory sensory neurons, and is predicted to be important to cilia.
Title: Tissue expression patterns identify mouse cilia genes.

identification of two mouse mutants, wimple (wim) and flexo (fxo), that lack ventral neural cell types and show other phenotypes characteristic of defects in Sonic hedgehog signalling
Title: Hedgehog signalling in the mouse requires intraflagellar transport proteins.

The following Ift172 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ift172 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu01943	NM_026298.5 Latest version!	Mus musculus intraflagellar transport 172 (Ift172), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1287.30 $1839.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ift172 ( NM_026298.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_080574.5 Mus musculus intraflagellar transport 172 (Ift172), mRNA.

Price & Availability↑

CloneID	OMu01943
Clone ID Related Accession (Same CDS sequence)	NM_026298.5
Accession Version	NM_026298.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5250bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	intraflagellar transport protein 172 homolog
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY317597.1, AY339616.1 and BC051928.1. On Dec 15, 2009 this sequence version replaced NM_026298.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC042583.1, BC066096.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGTTGA AGCACCTGAG GACCCTGCTG AGCCCTCAGG ATGGAGCTGC GAAGGTGACA 
TGCATGGCCT GGTCCCAGAA CAATGCTAAA TTTGCTGTCT GCACAGTGGA CCGAGTGGTA 
CTTCTGTATG ATGAACATGG GGAGCGGAGA GACAAGTTCT CCACCAAACC AGCTGACATG 
AAGTACGGCA GGAAAAGCTA CATGGTGAAA GGCATGGCTT TCTCTCCCGA TTCCACAAAA 
ATTGCCATAG GACAGACTGA CAACATCATC TATGTCTACA AGATTGGAGA AGATTGGGGA 
GACAAGAAGG TCATCTGCAA CAAGTTCATC CAGACGAGTG CTGTGACTTG TCTGCAGTGG 
CCTGCGGAAT ACGTCATTGT CTTTGGACTG GCAGAAGGCA AGGTTCGCTT AGCAAACACT 
AAAACTAACA AGTCATCTAC TATCTATGGG ACAGAGTCTT ACGTGGTAGC ACTGACAACC 
AACTGCTCTG GGAAGGGGAT CCTCTCGGGT CACGCAGACG GCACCATCGT CAGGTACTTC 
TTCGATGATG AAGGCTCTGG AGAGTCACAG GGAAAGTTGG TTAACCATCC ATGCCCACCC 
TATGCCTTGG CCTGGGCCAC CAACAGCATT GTGGCCGCTG GCTGTGACCG GAGGATTGTG 
GCCTATGGAA AGGAAGGCCA TGTGCTGCAG ACTTTTGACT ATAGCCGAGA CCCTCAGGAG 
CGGGAGTTCA CCACAGCTGC TGCAAGTCCT GGAGGCCAGT CTGTTGTGCT GGGAAGTTAT 
GACAGACTTC GGGTGTTCAA CTGGAGTCCT CGAAGAAGCA TCTGGGAAGA GGCGAAACCC 
AAGGAGATCG CTAACTTATA CACCGTCACC GCCCTGGCCT GGAAGCGGGA TGGCTCACGA 
CTCTGTGCGG GAACACTCTG TGGTGGGGTG GAACAGTTTG ACTGCTGCCT CCGAAGGAGC 
ATTTACAAGA ACAAGTTCGA GCTGACGTAT GTGGGCCCTA GCCAGGTGAT TGTGAAGAAC 
CTGTCTTCAG GGACCAGGGT GGTGCTCAAG TCACACTATG GCTATGAAGT AGAAGAGGTG 
AAAATCCTGG GAAAGGAACG TTACTTGGTG GCTCACACAT CAGACACACT GCTGCTGGGA 
GACCTGAACA CGAACCGCCT CAGCGAGATA GCCTGGCAGG GATCTGGGGG CAATGAGAAA 
TATTTCTTTG AAAATGAGAA TGTGTGCATG ATCTTCAATG CTGGAGAGCT GACCCTGGTG 
GAATATGGGA GTAACGACTC CTTGGGTTCT GTGCGCACCG AATTCATGAA CCCTCACCTC 
ATCAGCGTCC GTATCAATGA GCGCTGCCAG CGAGGAATGG AAGATAATAA GAAATTGGCT 
TATCTTGTTG ATATTAAGAC AATTGCTATA GTGGATCTGA TTGGTGGCTA CAACATTGGT 
ACTATCAGTC ACGAAAGCCG GGTGGATTGG CTAGAACTTA ACGAGACTGG ACACAAGCTT 
CTCTTTAGAG ACCGGAAACT TCGGCTGCAT CTATATGATA TTGAAAGCTG CTCTAAGACA 
ATGATCCTCA ACTTCTGCTC CTACGTGCAG TGGGTTCCGG GGAGTGATGT GCTGGTAGCT 
CAGAACCGAA ACAGCTTATG TGTATGGTAC AATATTGAGG CCCCTGAGAG AGTCACCATG 
TCCTCTATCA GGGGTGATGT TGTCGGTCTG GAGCGAGGTG GTGGGAAGAC AGAGGTCATG 
GTGACAGAAG GCGTGACTAC TGTGGCCTAC ACCCTGGACG AGGGCCTGAT TGAGTTTGGA 
ACAGCCATCG ATGATGGCAA CTACACCCGG GCTACAGCCT TCTTGGAGAC CCTGGAGATG 
ACTCCCGAAA CAGAGGCAAT GTGGAAAACC TTGAGCAAAC TGGCCCTGGA GGCAAGGCAG 
CTTCACACTG CTGAGAGGTG CTTCTCTGCT TTAGGTCATG TGGCCAAAGC TCGGTTCCTG 
CATGAGACCA ATGAGATTGC AGATCAGGTG TCCAGGGAAT ATGGTGGAGA AGGAACGGAC 
TTCTATCAGG TCCGAGCTCG TCTCGCCATG CTGGAGAAGA ACTACAAACT TGCTGAGATG 
ATCTTCTTGG AACAGAATGC TGTTGAGGAG GCCATGGACA TGTACCAAGA GTTGCACCGC 
TGGGAGGAAT GCATTGCTGT GGCTGAGGCC AAGGGACACC CAGCCCTGGA GAAGCTGCGC 
AGAGACTACT ATCAATGGTT AATGGACACA CAGCAAGAGG AACGAGCAGG TGAACTGCAG 
GAGAGCCAAG GGGATGGGCT GGCAGCTATC AGCCTCTACC TCAAAGCTGG GCTCCCTGCC 
AAAGCTGCGC GGCTGGTGCT GACCCGAGAG GAGCTGCTGG CCAACACAGA ACTAGTAGAA 
CATATCACCA CAGCCCTCAT CAAGGGAGAG CTCTATGAAA GGGCAGGTGA TCTCTTTGAG 
AAGATTCGAA ATCCACAGCG GGCCCTTGAG TGTTACTGTA AAGGCAATGC ATTCATGAAA 
GCTGTTGAGC TGGCTCGACT GGCCTTCCCA GTGGAGGTGG TGAGGCTAGA GGAGGCCTGG 
GGGGACCACT TGGTACAGCA AAAGCAGCTG GATGCGGCCA TCAACCACTA TATTGAAGCC 
AGGTGCTCCA TCAAAGCAAT TGAGGCTGCC CTGGGTGCCC GCCAGTGGAA GAAGGCAATT 
TATATATTAG ATCTCCAGGA TCGCAACACT GCATCTAAAT ACTATCCTCG TGTGGCTCAA 
CACTATGCGT CTCTGCAGGA GTATGAGATT GCTGAGGAGC TCTACACCAA AGGAGACCGG 
ACCAAAGACG CCATAGACAT GTACACCCAG GCCGGCCGCT GGGAGCAAGC CCACAAGCTG 
GCCATGAAAT GCATGCGACC CGAGGACGTG TCCGTGCTTT ACATCACACA AGCCCAGGAG 
ATGGAGAAGC AGGGCAAGTA CCGAGAGGCT GAGAGGCTGT ACGTGACGGT GGAGGAGCCT 
GATCTCGCCA TCACCATGTT CAAAAAGCAC AAGTTGTATG ACGACATGAT TCGCCTGGTG 
GGGAAGCACC ATCCAGACCT CCTCAGCGAC ACACACCTTC ACCTGGGCAA GGAGCTGGAG 
GCAGAAGGCC GACTGCAGGA GGCTGAGTAT CACTACCTTG AGGCCCAGGA ATGGAAGGCA 
ACTGTGAACA TGTACCGCTC CAGCGGGCTT TGGGAGGAGG CCTACCGGGT GGCCAAAGCC 
CATGGAGGAG CCAATGCCCA CAAACACGTG GCCTATCTGT GGGCCAAAAG CCTGGGAGGA 
GAGGCTGCAG TTAGACTACT TAACAAGCTG GGACTTCTGG AGGCCACCAT TGACCATGCT 
GCTGACAACT GCTCCTTTGA GTTTGCCTTT GAACTCTCTC GGCTAGCCTT CAAGCACAAA 
GCCCCAGAGA TTCATCTCAA ATACGCCATG TACCTGGAAG ATGAGGGCAA ATTTGAAGAG 
GCTGAAGCTG AATTCATCCG AGCTGGTAAA CCCAAAGAGG CGGTCCTCAT GTTTGTCCAC 
AACCAGGACT GGGAGGCAGC TCAGCGCGTG GCTGAGGCCC ACGATCCTGA CAGCGTTGCT 
GAGGTACTTG TAGGTCAAGC CCGTGGGGCC TTGGAGGAGA AGGACTTTCA GAAGGCAGAA 
GGGTTACTAC TTCGGGCCCA GAGACCAGGT CTGGCTCTCA ATTATTATAA GGAGGCTGGA 
CTCTGGAGCG ACGCTCTGAG AATCTGCAAG GACTATGTGC CTGGCCAGCT AGAGGCCCTA 
CAGGAAGAGT ATGAGCGAGA AGCTACCAAG AAGGGAGGCA GAGGGGTGGA GGGACTTGTA 
GAGCAAGCAC GGCAGTGGGA ACAGGCTGGA GAGTATAGCC GTGCAGTGGA CTGCTACCTC 
AAAGTTCGCG ACTCAGGAAG CAGTGGCCTG ATGGAGAAGT GCTGGATGAA GGCGGCTGAA 
CTGTCCATCA AGTTCCTGCC TCCCCAGCGC AGTCTGGAAG TCGTCCGGGT TGTAGGGCCC 
CAGCTGATCG GGATTGGAAA GCACAGTGCT GCTGCAGAAC TCTATCTGAA CCTGGACCTT 
GTCAAAGAAG CAATCGATGC TTTCATTGAG GGGGAAGAGT GGAACAAGGC CAAGCGCGTG 
GCCAAAGAGC TAGATCCCCG GTATGAGGAC TATGTGGATC AGCACTATAA GGAGTTCCTC 
AAGAACCAGG GCAAAGTGGA TTCGCTGGTG GGTGTGGACG TAGTAGCTGC CTTGGACCTG 
TATGTGGAGC AGGGCCAGTG GGATAAATGC ATTGAAACAG CTACCAAGCA GAACTACAAG 
ATTCTGCACA AGTATGTGGC TTTGTATGCA ACTCATCTGA TCCGAGAAGG TGGCTATGCC 
CAGGCACTGG CCCTCTATGT GCAACACGGA GCCCCTGCAA ACCCACAGAA TTTCAACATC 
TACAAAAGGA TCTTCACTGA CATGGTTAGC TCTCCTGGGA CCAACAATGC TGAGGCCTAC 
CATAGCTGGG CTGATCTTCG GGATGTCCTC TTCAACCTGT GTGAAAACCT GGTGAAGTCC 
AGTGAGGCAA ACTCTGCAGC GCATGAGGAG TTTGAGATGA TGCTGCTGAT CTCTCATTAC 
TACGCTACGC GATCTGCAGC CCAGAGTATC AAACAGCTGG AAACCGTAGC TGCCAGGCTT 
TCTGTTTCGC TCTTGCGCCA CACCCAGTTA CTCCCTGCAG ACAAGGCCTT TTATGAAGCG 
GGCACTGCTG CCAAGGAAGT TGGCTGGGAG AACATGGCTT TCATTTTCCT CAATCGGTTC 
TTGGATCTGA CTGATGCAAT CGAAGAAGGG ACCCTGGATG CCCTGGACCA TTCAGATTTT 
CAGGATACTG ACATTCCCTT TGAGGTGCCG CTCCCAGCCA AGCAGCACGT CCCGGAGGCC 
CAGAGAGAAG AGGTCCGAGA CTGGGTGCTC ACAGTCTCGA TGGACCAGAG ACTGGAGCAG 
GTTCTGCCCC GGGATGAGCG AGGTGTCTAT GAGGCTTCCC TAGTTGCCGC GAGCACTGGA 
GTTCGGGCAC TGCCCTGCCT CATCACAGGT TACCCCATTT TGAGGAACAA AATTGAATTT 
AAGCGGCCTG GGAAGGCTGC TAACAAAGAC AACTGGAACA AGTTTCTTAT GGCCATCAAG 
ACCTCTCACA GCCCAGTGTG CCAGGACGTG CTCAAGTTCA TCAGCCAGTG GTGCGGTGGT 
CTCCCTAGCA CCAGCTTTTC CTTTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_080574.5
CDS	103..5352
Translation	MQLKHLRTLLSPQDGAAKVTCMAWSQNNAKFAVCTVDRVVLLYDEHGERRDKFSTKPADMKYGRKSYMVKGMAFSPDSTKIAIGQTDNIIYVYKIGEDWGDKKVICNKFIQTSAVTCLQWPAEYVIVFGLAEGKVRLANTKTNKSSTIYGTESYVVALTTNCSGKGILSGHADGTIVRYFFDDEGSGESQGKLVNHPCPPYALAWATNSIVAAGCDRRIVAYGKEGHVLQTFDYSRDPQEREFTTAAASPGGQSVVLGSYDRLRVFNWSPRRSIWEEAKPKEIANLYTVTALAWKRDGSRLCAGTLCGGVEQFDCCLRRSIYKNKFELTYVGPSQVIVKNLSSGTRVVLKSHYGYEVEEVKILGKERYLVAHTSDTLLLGDLNTNRLSEIAWQGSGGNEKYFFENENVCMIFNAGELTLVEYGSNDSLGSVRTEFMNPHLISVRINERCQRGMEDNKKLAYLVDIKTIAIVDLIGGYNIGTISHESRVDWLELNETGHKLLFRDRKLRLHLYDIESCSKTMILNFCSYVQWVPGSDVLVAQNRNSLCVWYNIEAPERVTMSSIRGDVVGLERGGGKTEVMVTEGVTTVAYTLDEGLIEFGTAIDDGNYTRATAFLETLEMTPETEAMWKTLSKLALEARQLHTAERCFSALGHVAKARFLHETNEIADQVSREYGGEGTDFYQVRARLAMLEKNYKLAEMIFLEQNAVEEAMDMYQELHRWEECIAVAEAKGHPALEKLRRDYYQWLMDTQQEERAGELQESQGDGLAAISLYLKAGLPAKAARLVLTREELLANTELVEHITTALIKGELYERAGDLFEKIRNPQRALECYCKGNAFMKAVELARLAFPVEVVRLEEAWGDHLVQQKQLDAAINHYIEARCSIKAIEAALGARQWKKAIYILDLQDRNTASKYYPRVAQHYASLQEYEIAEELYTKGDRTKDAIDMYTQAGRWEQAHKLAMKCMRPEDVSVLYITQAQEMEKQGKYREAERLYVTVEEPDLAITMFKKHKLYDDMIRLVGKHHPDLLSDTHLHLGKELEAEGRLQEAEYHYLEAQEWKATVNMYRSSGLWEEAYRVAKAHGGANAHKHVAYLWAKSLGGEAAVRLLNKLGLLEATIDHAADNCSFEFAFELSRLAFKHKAPEIHLKYAMYLEDEGKFEEAEAEFIRAGKPKEAVLMFVHNQDWEAAQRVAEAHDPDSVAEVLVGQARGALEEKDFQKAEGLLLRAQRPGLALNYYKEAGLWSDALRICKDYVPGQLEALQEEYEREATKKGGRGVEGLVEQARQWEQAGEYSRAVDCYLKVRDSGSSGLMEKCWMKAAELSIKFLPPQRSLEVVRVVGPQLIGIGKHSAAAELYLNLDLVKEAIDAFIEGEEWNKAKRVAKELDPRYEDYVDQHYKEFLKNQGKVDSLVGVDVVAALDLYVEQGQWDKCIETATKQNYKILHKYVALYATHLIREGGYAQALALYVQHGAPANPQNFNIYKRIFTDMVSSPGTNNAEAYHSWADLRDVLFNLCENLVKSSEANSAAHEEFEMMLLISHYYATRSAAQSIKQLETVAARLSVSLLRHTQLLPADKAFYEAGTAAKEVGWENMAFIFLNRFLDLTDAIEEGTLDALDHSDFQDTDIPFEVPLPAKQHVPEAQREEVRDWVLTVSMDQRLEQVLPRDERGVYEASLVAASTGVRALPCLITGYPILRNKIEFKRPGKAANKDNWNKFLMAIKTSHSPVCQDVLKFISQWCGGLPSTSFSFQ

Target ORF information:

RefSeq Version	NM_026298.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus intraflagellar transport 172 (Ift172), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_026298.5

ORF Insert Sequence:

ATGCAGTTGA AGCACCTGAG GACCCTGCTG AGCCCTCAGG ATGGAGCTGC GAAGGTGACA 
TGCATGGCCT GGTCCCAGAA CAATGCTAAA TTTGCTGTCT GCACAGTGGA CCGAGTGGTA 
CTTCTGTATG ATGAACATGG GGAGCGGAGA GACAAGTTCT CCACCAAACC AGCTGACATG 
AAGTACGGCA GGAAAAGCTA CATGGTGAAA GGCATGGCTT TCTCTCCCGA TTCCACAAAA 
ATTGCCATAG GACAGACTGA CAACATCATC TATGTCTACA AGATTGGAGA AGATTGGGGA 
GACAAGAAGG TCATCTGCAA CAAGTTCATC CAGACGAGTG CTGTGACTTG TCTGCAGTGG 
CCTGCGGAAT ACGTCATTGT CTTTGGACTG GCAGAAGGCA AGGTTCGCTT AGCAAACACT 
AAAACTAACA AGTCATCTAC TATCTATGGG ACAGAGTCTT ACGTGGTAGC ACTGACAACC 
AACTGCTCTG GGAAGGGGAT CCTCTCGGGT CACGCAGACG GCACCATCGT CAGGTACTTC 
TTCGATGATG AAGGCTCTGG AGAGTCACAG GGAAAGTTGG TTAACCATCC ATGCCCACCC 
TATGCCTTGG CCTGGGCCAC CAACAGCATT GTGGCCGCTG GCTGTGACCG GAGGATTGTG 
GCCTATGGAA AGGAAGGCCA TGTGCTGCAG ACTTTTGACT ATAGCCGAGA CCCTCAGGAG 
CGGGAGTTCA CCACAGCTGC TGCAAGTCCT GGAGGCCAGT CTGTTGTGCT GGGAAGTTAT 
GACAGACTTC GGGTGTTCAA CTGGAGTCCT CGAAGAAGCA TCTGGGAAGA GGCGAAACCC 
AAGGAGATCG CTAACTTATA CACCGTCACC GCCCTGGCCT GGAAGCGGGA TGGCTCACGA 
CTCTGTGCGG GAACACTCTG TGGTGGGGTG GAACAGTTTG ACTGCTGCCT CCGAAGGAGC 
ATTTACAAGA ACAAGTTCGA GCTGACGTAT GTGGGCCCTA GCCAGGTGAT TGTGAAGAAC 
CTGTCTTCAG GGACCAGGGT GGTGCTCAAG TCACACTATG GCTATGAAGT AGAAGAGGTG 
AAAATCCTGG GAAAGGAACG TTACTTGGTG GCTCACACAT CAGACACACT GCTGCTGGGA 
GACCTGAACA CGAACCGCCT CAGCGAGATA GCCTGGCAGG GATCTGGGGG CAATGAGAAA 
TATTTCTTTG AAAATGAGAA TGTGTGCATG ATCTTCAATG CTGGAGAGCT GACCCTGGTG 
GAATATGGGA GTAACGACTC CTTGGGTTCT GTGCGCACCG AATTCATGAA CCCTCACCTC 
ATCAGCGTCC GTATCAATGA GCGCTGCCAG CGAGGAATGG AAGATAATAA GAAATTGGCT 
TATCTTGTTG ATATTAAGAC AATTGCTATA GTGGATCTGA TTGGTGGCTA CAACATTGGT 
ACTATCAGTC ACGAAAGCCG GGTGGATTGG CTAGAACTTA ACGAGACTGG ACACAAGCTT 
CTCTTTAGAG ACCGGAAACT TCGGCTGCAT CTATATGATA TTGAAAGCTG CTCTAAGACA 
ATGATCCTCA ACTTCTGCTC CTACGTGCAG TGGGTTCCGG GGAGTGATGT GCTGGTAGCT 
CAGAACCGAA ACAGCTTATG TGTATGGTAC AATATTGAGG CCCCTGAGAG AGTCACCATG 
TCCTCTATCA GGGGTGATGT TGTCGGTCTG GAGCGAGGTG GTGGGAAGAC AGAGGTCATG 
GTGACAGAAG GCGTGACTAC TGTGGCCTAC ACCCTGGACG AGGGCCTGAT TGAGTTTGGA 
ACAGCCATCG ATGATGGCAA CTACACCCGG GCTACAGCCT TCTTGGAGAC CCTGGAGATG 
ACTCCCGAAA CAGAGGCAAT GTGGAAAACC TTGAGCAAAC TGGCCCTGGA GGCAAGGCAG 
CTTCACACTG CTGAGAGGTG CTTCTCTGCT TTAGGTCATG TGGCCAAAGC TCGGTTCCTG 
CATGAGACCA ATGAGATTGC AGATCAGGTG TCCAGGGAAT ATGGTGGAGA AGGAACGGAC 
TTCTATCAGG TCCGAGCTCG TCTCGCCATG CTGGAGAAGA ACTACAAACT TGCTGAGATG 
ATCTTCTTGG AACAGAATGC TGTTGAGGAG GCCATGGACA TGTACCAAGA GTTGCACCGC 
TGGGAGGAAT GCATTGCTGT GGCTGAGGCC AAGGGACACC CAGCCCTGGA GAAGCTGCGC 
AGAGACTACT ATCAATGGTT AATGGACACA CAGCAAGAGG AACGAGCAGG TGAACTGCAG 
GAGAGCCAAG GGGATGGGCT GGCAGCTATC AGCCTCTACC TCAAAGCTGG GCTCCCTGCC 
AAAGCTGCGC GGCTGGTGCT GACCCGAGAG GAGCTGCTGG CCAACACAGA ACTAGTAGAA 
CATATCACCA CAGCCCTCAT CAAGGGAGAG CTCTATGAAA GGGCAGGTGA TCTCTTTGAG 
AAGATTCGAA ATCCACAGCG GGCCCTTGAG TGTTACTGTA AAGGCAATGC ATTCATGAAA 
GCTGTTGAGC TGGCTCGACT GGCCTTCCCA GTGGAGGTGG TGAGGCTAGA GGAGGCCTGG 
GGGGACCACT TGGTACAGCA AAAGCAGCTG GATGCGGCCA TCAACCACTA TATTGAAGCC 
AGGTGCTCCA TCAAAGCAAT TGAGGCTGCC CTGGGTGCCC GCCAGTGGAA GAAGGCAATT 
TATATATTAG ATCTCCAGGA TCGCAACACT GCATCTAAAT ACTATCCTCG TGTGGCTCAA 
CACTATGCGT CTCTGCAGGA GTATGAGATT GCTGAGGAGC TCTACACCAA AGGAGACCGG 
ACCAAAGACG CCATAGACAT GTACACCCAG GCCGGCCGCT GGGAGCAAGC CCACAAGCTG 
GCCATGAAAT GCATGCGACC CGAGGACGTG TCCGTGCTTT ACATCACACA AGCCCAGGAG 
ATGGAGAAGC AGGGCAAGTA CCGAGAGGCT GAGAGGCTGT ACGTGACGGT GGAGGAGCCT 
GATCTCGCCA TCACCATGTT CAAAAAGCAC AAGTTGTATG ACGACATGAT TCGCCTGGTG 
GGGAAGCACC ATCCAGACCT CCTCAGCGAC ACACACCTTC ACCTGGGCAA GGAGCTGGAG 
GCAGAAGGCC GACTGCAGGA GGCTGAGTAT CACTACCTTG AGGCCCAGGA ATGGAAGGCA 
ACTGTGAACA TGTACCGCTC CAGCGGGCTT TGGGAGGAGG CCTACCGGGT GGCCAAAGCC 
CATGGAGGAG CCAATGCCCA CAAACACGTG GCCTATCTGT GGGCCAAAAG CCTGGGAGGA 
GAGGCTGCAG TTAGACTACT TAACAAGCTG GGACTTCTGG AGGCCACCAT TGACCATGCT 
GCTGACAACT GCTCCTTTGA GTTTGCCTTT GAACTCTCTC GGCTAGCCTT CAAGCACAAA 
GCCCCAGAGA TTCATCTCAA ATACGCCATG TACCTGGAAG ATGAGGGCAA ATTTGAAGAG 
GCTGAAGCTG AATTCATCCG AGCTGGTAAA CCCAAAGAGG CGGTCCTCAT GTTTGTCCAC 
AACCAGGACT GGGAGGCAGC TCAGCGCGTG GCTGAGGCCC ACGATCCTGA CAGCGTTGCT 
GAGGTACTTG TAGGTCAAGC CCGTGGGGCC TTGGAGGAGA AGGACTTTCA GAAGGCAGAA 
GGGTTACTAC TTCGGGCCCA GAGACCAGGT CTGGCTCTCA ATTATTATAA GGAGGCTGGA 
CTCTGGAGCG ACGCTCTGAG AATCTGCAAG GACTATGTGC CTGGCCAGCT AGAGGCCCTA 
CAGGAAGAGT ATGAGCGAGA AGCTACCAAG AAGGGAGGCA GAGGGGTGGA GGGACTTGTA 
GAGCAAGCAC GGCAGTGGGA ACAGGCTGGA GAGTATAGCC GTGCAGTGGA CTGCTACCTC 
AAAGTTCGCG ACTCAGGAAG CAGTGGCCTG ATGGAGAAGT GCTGGATGAA GGCGGCTGAA 
CTGTCCATCA AGTTCCTGCC TCCCCAGCGC AGTCTGGAAG TCGTCCGGGT TGTAGGGCCC 
CAGCTGATCG GGATTGGAAA GCACAGTGCT GCTGCAGAAC TCTATCTGAA CCTGGACCTT 
GTCAAAGAAG CAATCGATGC TTTCATTGAG GGGGAAGAGT GGAACAAGGC CAAGCGCGTG 
GCCAAAGAGC TAGATCCCCG GTATGAGGAC TATGTGGATC AGCACTATAA GGAGTTCCTC 
AAGAACCAGG GCAAAGTGGA TTCGCTGGTG GGTGTGGACG TAGTAGCTGC CTTGGACCTG 
TATGTGGAGC AGGGCCAGTG GGATAAATGC ATTGAAACAG CTACCAAGCA GAACTACAAG 
ATTCTGCACA AGTATGTGGC TTTGTATGCA ACTCATCTGA TCCGAGAAGG TGGCTATGCC 
CAGGCACTGG CCCTCTATGT GCAACACGGA GCCCCTGCAA ACCCACAGAA TTTCAACATC 
TACAAAAGGA TCTTCACTGA CATGGTTAGC TCTCCTGGGA CCAACAATGC TGAGGCCTAC 
CATAGCTGGG CTGATCTTCG GGATGTCCTC TTCAACCTGT GTGAAAACCT GGTGAAGTCC 
AGTGAGGCAA ACTCTGCAGC GCATGAGGAG TTTGAGATGA TGCTGCTGAT CTCTCATTAC 
TACGCTACGC GATCTGCAGC CCAGAGTATC AAACAGCTGG AAACCGTAGC TGCCAGGCTT 
TCTGTTTCGC TCTTGCGCCA CACCCAGTTA CTCCCTGCAG ACAAGGCCTT TTATGAAGCG 
GGCACTGCTG CCAAGGAAGT TGGCTGGGAG AACATGGCTT TCATTTTCCT CAATCGGTTC 
TTGGATCTGA CTGATGCAAT CGAAGAAGGG ACCCTGGATG CCCTGGACCA TTCAGATTTT 
CAGGATACTG ACATTCCCTT TGAGGTGCCG CTCCCAGCCA AGCAGCACGT CCCGGAGGCC 
CAGAGAGAAG AGGTCCGAGA CTGGGTGCTC ACAGTCTCGA TGGACCAGAG ACTGGAGCAG 
GTTCTGCCCC GGGATGAGCG AGGTGTCTAT GAGGCTTCCC TAGTTGCCGC GAGCACTGGA 
GTTCGGGCAC TGCCCTGCCT CATCACAGGT TACCCCATTT TGAGGAACAA AATTGAATTT 
AAGCGGCCTG GGAAGGCTGC TAACAAAGAC AACTGGAACA AGTTTCTTAT GGCCATCAAG 
ACCTCTCACA GCCCAGTGTG CCAGGACGTG CTCAAGTTCA TCAGCCAGTG GTGCGGTGGT 
CTCCCTAGCA CCAGCTTTTC CTTTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Proper ciliary assembly is critical for restricting Hedgehog signaling during early eye development in mice.
Developmental biology430(1)32-40(2017 10)
Burnett JB,Lupu FI,Eggenschwiler JT

The Meckel syndrome- associated protein MKS1 functionally interacts with components of the BBSome and IFT complexes to mediate ciliary trafficking and hedgehog signaling.
PloS one12(3)e0173399(2017)
Goetz SC,Bangs F,Barrington CL,Katsanis N,Anderson KV

Cilia gene mutations cause atrioventricular septal defects by multiple mechanisms.
Human molecular genetics25(14)3011-3028(2016 07)
Burnicka-Turek O,Steimle JD,Huang W,Felker L,Kamp A,Kweon J,Peterson M,Reeves RH,Maslen CL,Gruber PJ,Yang XH,Shendure J,Moskowitz IP

Does 3D Phenotyping Yield Substantial Insights in the Genetics of the Mouse Mandible Shape?
G3 (Bethesda, Md.)6(5)1153-63(2016 05)
Navarro N,Maga AM

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Ift172 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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