Mastl cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mastl
Entrez Gene ID	67121
Full Name	microtubule associated serine/threonine kinase-like
Synonyms	2700091H24Rik,C88295,GW,GWL,MAST-L,THC2
General protein information	Preferred Names microtubule associated serine/threonine kinase-like Names serine/threonine-protein kinase greatwall microtubule-associated serine/threonine-protein kinase-like
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 A3

mRNA and Protein(s)

mRNA	Protein	Name
XM_017319214.1	XP_017174703.1	serine/threonine-protein kinase greatwall isoform X2
XM_006498239.3	XP_006498302.1	serine/threonine-protein kinase greatwall isoform X1
XM_030251977.1	XP_030107837.1	serine/threonine-protein kinase greatwall isoform X1
NM_025979.4	NP_080255.3	serine/threonine-protein kinase greatwall

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Mastl	NP_080255.3
Rattus norvegicus (Norway rat)	Mastl	NP_001100839.1
Gallus gallus (chicken)	MASTL	XP_418589.2
Homo sapiens (human)	MASTL	NP_001165774.1
Pan troglodytes (chimpanzee)	MASTL	XP_001160397.3
	MASTL	XP_001104617.1
Canis lupus familiaris (dog)	MASTL	XP_848866.1
Bos taurus (cattle)	MASTL	NP_001107237.1
Danio rerio (zebrafish)	mastl	NP_001003609.2
Xenopus tropicalis (tropical clawed frog)	mastl	NP_001120546.1

Gene Ontology
Bibliography

Related articles in PubMed

Loss of the Greatwall Kinase Weakens the Spindle Assembly Checkpoint.
Diril MK, Bisteau X, Kitagawa M, Caldez MJ, Wee S, Gunaratne J, Lee SH, Kaldis P
PLoS genetics12(9)e1006310(2016 Sep)

Mastl is required for timely activation of APC/C in meiosis I and Cdk1 reactivation in meiosis II.
Adhikari D, Diril MK, Busayavalasa K, Risal S, Nakagawa S, Lindkvist R, Shen Y, Coppola V, Tessarollo L, Kudo NR, Kaldis P, Liu K
The Journal of cell biology206(7)843-53(2014 Sep)

Greatwall is essential to prevent mitotic collapse after nuclear envelope breakdown in mammals.
?lvarez-Fern?ndez M, S?nchez-Mart?nez R, Sanz-Castillo B, Gan PP, Sanz-Flores M, Trakala M, Ruiz-Torres M, Lorca T, Castro A, Malumbres M
Proceedings of the National Academy of Sciences of the United States of America110(43)17374-9(2013 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

using in vitro dephosphorylation assays, we demonstrate that Mastl promotes persistent MPS1 phosphorylation by inhibiting PP2A/B55-mediated MPS1 dephosphorylation rather than affecting Cdk1 kinase activity. Our findings establish a key regulatory function of the Greatwall kinase/Mastl - PP2A/B55 pathway in preventing premature SAC silencing
Title: Loss of the Greatwall Kinase Weakens the Spindle Assembly Checkpoint.

Mastl is required for the timely activation of anaphase-promoting complex/cyclosome to allow meiosis I exit and for the rapid rise of Cdk1 activity.
Title: Mastl is required for timely activation of APC/C in meiosis I and Cdk1 reactivation in meiosis II.

Data show that Mastl (Greatwall)-null cells display mitotic collapse after nuclear envelope breakdown (NEB) characterized by defective chromosome condensation and prometaphase arrest.
Title: Greatwall is essential to prevent mitotic collapse after nuclear envelope breakdown in mammals.

The following Mastl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mastl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu21789	NM_025979.4 Latest version!	Mus musculus microtubule associated serine/threonine kinase-like (Mastl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OMu29679	XM_006498239.3 Latest version!	Mus musculus microtubule associated serine/threonine kinase-like (Mastl), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu29679	XM_030251977.1 Latest version!	Mus musculus microtubule associated serine/threonine kinase-like (Mastl), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu80391	XM_017319214.1 Latest version!	Mus musculus microtubule associated serine/threonine kinase-like (Mastl), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mastl ( NM_025979.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_080255.3 Mus musculus microtubule associated serine/threonine kinase-like (Mastl), mRNA.

Price & Availability↑

CloneID	OMu21789
Clone ID Related Accession (Same CDS sequence)	NM_025979.4
Accession Version	NM_025979.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2598bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase greatwall
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL845257.2, AK030140.1, CN536399.1 and CD546688.1. On Jul 21, 2009 this sequence version replaced NM_025979.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK030140.1, BC103779.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164143 [ECO:0000348] ##Evidence-Data-END##

ATGGAGTCCG CCTCGGCAAG TGAGGAGAAT GAAGGAGGTG CGGCGATAGA GGAGTGCGTG 
AGTAGGATCC CGGTACCAAG ACCACCCTCC ATTGAGGAGT TCACCATAGT GAAGCCCATT 
AGCCGTGGTG CATTCGGGAA AGTGTACCTG GGACAGAAAG GCGGCAAGTT GTACGCAGTG 
AAGGTTGTCA AAAAGGCAGA CATGATCAAT AAAAATATGA CTCATCAGGT ACAAGCTGAG 
AGAGATGCCC TGGCACTGAG CAAAAGCCCT TTTGTTGTGC ATCTCTATTA CTCACTGCAG 
TCAGCAAGCA ACATCTACTT GATAATGGAG TATCTTATTG GTGGAGATGT CAAGTCTCTC 
CTACATATAT ATGGTTATTT TGATGAAGAG ATGGCTATAA AATATATTTC TGAAGTAGCA 
TTGGCTTTAG ACTACCTGCA CAGACATGGA ATCATCCATA GGGATTTGAA ACCGGACAAT 
ATGCTTATTT CCAATGAGGG TCATATTAAA CTGACAGATT TTGGCCTTTC CAAAGTTACT 
TTGAATAGAG ACATCAATAT GATGGATATT CTCACAACAC CATCAATGTC TAAACCTAAG 
CAAGATTATT CAAGAACTCC AGGACAAGTC TTATCTCTCA TCAGTTCTTT GGGATTTTTC 
ACACCAGTTG GAGAAAAAGA TCAAGACTCT GCAAACATGT TTTCAGCCCC TAAGTCTGCT 
GCACAACTTT CTCGAGGATT CATTTGTCCT ATGTCTGTAG ATCAGAAGGA GCCGACTTCT 
TATTCGAGCA AACTGCTAAA ATCATGTTTT GAAACCCTTT CATCCAATCC AGAAATACCT 
GTGAAATGTC TGACTTCTAA TCTACTCCAG TGTAGGAAAA GACTGGGTAC CTCAAGTACT 
AGTAGCCAGT CTCATACCTT CGTATCCAGT GTGGAGTCAG AATGCCACAG CAATCCCAAA 
TGGGAAAGAG ACTGCCAGAG TACCGAATCA TCAGGCTGTG CAATGAGCTG GAATGCAGTT 
GAAATGCTGT ATGCAAAATC TACAAGTGCC ATCAAGACAA AAACAGAACT GGAATTAGCA 
CTTTCTCCCA TTCATGACAG CAGTGCCATT CCTGCTGCTG GAAGCAACCA GGTGACCCTC 
CCTAGAAAAT GCTTCAGGGA AATCTCTTGG GAAGCAAGGG ACCCAGATAA TGAGAACATG 
ACCATTGACA AGGGACAGTC TGGTTTCTGT CAGTCCAGTC AGAGGTCTGT AAATTCTAGT 
GCTACATCTG AAGAACACCT TGGTAAAAGA AATTATAAAA GAAATTTCCA CTTGGTTGAC 
TCCAGTCCTT GTCAGGAAAT TATGCAAAGT AAAAAAAACT GTACAGAGTA TGAGGCCAAT 
AAGGAAAGGC AAGGCTGCCG TGCAAATCAA AGTACAGGCT TGACAACCGA AGTCCAGAAC 
CTGAAGCTAT CAGGGTGTGA AAGTCAGCAA CTTGACTATG CCAATAAGGA GAACATTGTC 
ACCTATTTAA CTGACAGACA AACCCCAGAA AAACTGCATA TACCAACGAT AGCAAAGAAC 
CTTATGTCTG AACTAGATGA AGACTGTGAG CTGAGCAGTA AAAAGGACTG CCTCAGTTCT 
AATTCTGTAT GTTCTGATGA GGATAGAGCT CTTAAAACTA CCTGTGTGGA TTCGGATTCA 
TCTTTTCCTG GAGTGTCTAT GATGGAGAGT TCACTGGAAA TTCAGGCCTT AGAACCAGAT 
AAAAGCATCA GAGACTACTC TTTTGAAGAA CCAAACACTG AAGATCTATT TGTGTTACCA 
AAATGCCAAG AAAATTCCTT GCCACAAGAT GACTGCCATG CTTGTATCCA AGACAGTAGC 
CAAGTGTCAG CTCATCCCTC AAAGGCGCCC AAAGCATTGA CCTCTAAAAT CAACGTCGTG 
GCTTTTCGAA GTTTTAACAG TCATATTAAC GCATCTACTA ATTCGGAACC ATCCAAAATC 
AGCATAACTT CTTTAGATGC TATGGACATT TCCTGTGATT ACAGTGGTTC ATACCCCATG 
GCTGTGAGCC CTACTGAGAA AGGAAGGCAC TACACGTCAC ATCAGACTCC AAATCAGGTC 
AAGTTGGGAA CATCATACAG AACTCCAAAG AGTGTGAGAA GAGGGGCAGC CCCAGTAGAT 
GACGGGCGAA TTCTAGGGAC TCCAGATTAC CTGGCACCTG AGCTCTTACT GGGTACAGCC 
CATGGTCCTG CAGTAGACTG GTGGGCTCTT GGAGTTTGCT TGTTCGAATT TCTGACAGGA 
ATTCCCCCTT TCAATGATGA AACACCACAA CAAGTATTCC AGAATATTTT AAAAAGAGAT 
ATCCCATGGC CAGAAGGCGA AGAAAAGCTA TCTGATAATG CTCAAAGTGC AATGGACATG 
CTTTTAACCA TTGATGATTC AAAGAGAGCT GGAATGAGAG AACTAAAACA GCATCCTCTC 
TTCAGTGAAG TGGACTGGGA AAATCTGCAG CATCAGACTA TGCCTTTCGT ACCCCAACCA 
GACGACGAAA CAGATACATC CTATTTTGAA GCCAGAAATA ATGCTCAACA TCTGACCATA 
TCTGGGTTTA GTCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_080255.3
CDS	164..2761
Translation	MESASASEENEGGAAIEECVSRIPVPRPPSIEEFTIVKPISRGAFGKVYLGQKGGKLYAVKVVKKADMINKNMTHQVQAERDALALSKSPFVVHLYYSLQSASNIYLIMEYLIGGDVKSLLHIYGYFDEEMAIKYISEVALALDYLHRHGIIHRDLKPDNMLISNEGHIKLTDFGLSKVTLNRDINMMDILTTPSMSKPKQDYSRTPGQVLSLISSLGFFTPVGEKDQDSANMFSAPKSAAQLSRGFICPMSVDQKEPTSYSSKLLKSCFETLSSNPEIPVKCLTSNLLQCRKRLGTSSTSSQSHTFVSSVESECHSNPKWERDCQSTESSGCAMSWNAVEMLYAKSTSAIKTKTELELALSPIHDSSAIPAAGSNQVTLPRKCFREISWEARDPDNENMTIDKGQSGFCQSSQRSVNSSATSEEHLGKRNYKRNFHLVDSSPCQEIMQSKKNCTEYEANKERQGCRANQSTGLTTEVQNLKLSGCESQQLDYANKENIVTYLTDRQTPEKLHIPTIAKNLMSELDEDCELSSKKDCLSSNSVCSDEDRALKTTCVDSDSSFPGVSMMESSLEIQALEPDKSIRDYSFEEPNTEDLFVLPKCQENSLPQDDCHACIQDSSQVSAHPSKAPKALTSKINVVAFRSFNSHINASTNSEPSKISITSLDAMDISCDYSGSYPMAVSPTEKGRHYTSHQTPNQVKLGTSYRTPKSVRRGAAPVDDGRILGTPDYLAPELLLGTAHGPAVDWWALGVCLFEFLTGIPPFNDETPQQVFQNILKRDIPWPEGEEKLSDNAQSAMDMLLTIDDSKRAGMRELKQHPLFSEVDWENLQHQTMPFVPQPDDETDTSYFEARNNAQHLTISGFSL

Target ORF information:

RefSeq Version	NM_025979.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus microtubule associated serine/threonine kinase-like (Mastl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025979.4

ORF Insert Sequence:

ATGGAGTCCG CCTCGGCAAG TGAGGAGAAT GAAGGAGGTG CGGCGATAGA GGAGTGCGTG 
AGTAGGATCC CGGTACCAAG ACCACCCTCC ATTGAGGAGT TCACCATAGT GAAGCCCATT 
AGCCGTGGTG CATTCGGGAA AGTGTACCTG GGACAGAAAG GCGGCAAGTT GTACGCAGTG 
AAGGTTGTCA AAAAGGCAGA CATGATCAAT AAAAATATGA CTCATCAGGT ACAAGCTGAG 
AGAGATGCCC TGGCACTGAG CAAAAGCCCT TTTGTTGTGC ATCTCTATTA CTCACTGCAG 
TCAGCAAGCA ACATCTACTT GATAATGGAG TATCTTATTG GTGGAGATGT CAAGTCTCTC 
CTACATATAT ATGGTTATTT TGATGAAGAG ATGGCTATAA AATATATTTC TGAAGTAGCA 
TTGGCTTTAG ACTACCTGCA CAGACATGGA ATCATCCATA GGGATTTGAA ACCGGACAAT 
ATGCTTATTT CCAATGAGGG TCATATTAAA CTGACAGATT TTGGCCTTTC CAAAGTTACT 
TTGAATAGAG ACATCAATAT GATGGATATT CTCACAACAC CATCAATGTC TAAACCTAAG 
CAAGATTATT CAAGAACTCC AGGACAAGTC TTATCTCTCA TCAGTTCTTT GGGATTTTTC 
ACACCAGTTG GAGAAAAAGA TCAAGACTCT GCAAACATGT TTTCAGCCCC TAAGTCTGCT 
GCACAACTTT CTCGAGGATT CATTTGTCCT ATGTCTGTAG ATCAGAAGGA GCCGACTTCT 
TATTCGAGCA AACTGCTAAA ATCATGTTTT GAAACCCTTT CATCCAATCC AGAAATACCT 
GTGAAATGTC TGACTTCTAA TCTACTCCAG TGTAGGAAAA GACTGGGTAC CTCAAGTACT 
AGTAGCCAGT CTCATACCTT CGTATCCAGT GTGGAGTCAG AATGCCACAG CAATCCCAAA 
TGGGAAAGAG ACTGCCAGAG TACCGAATCA TCAGGCTGTG CAATGAGCTG GAATGCAGTT 
GAAATGCTGT ATGCAAAATC TACAAGTGCC ATCAAGACAA AAACAGAACT GGAATTAGCA 
CTTTCTCCCA TTCATGACAG CAGTGCCATT CCTGCTGCTG GAAGCAACCA GGTGACCCTC 
CCTAGAAAAT GCTTCAGGGA AATCTCTTGG GAAGCAAGGG ACCCAGATAA TGAGAACATG 
ACCATTGACA AGGGACAGTC TGGTTTCTGT CAGTCCAGTC AGAGGTCTGT AAATTCTAGT 
GCTACATCTG AAGAACACCT TGGTAAAAGA AATTATAAAA GAAATTTCCA CTTGGTTGAC 
TCCAGTCCTT GTCAGGAAAT TATGCAAAGT AAAAAAAACT GTACAGAGTA TGAGGCCAAT 
AAGGAAAGGC AAGGCTGCCG TGCAAATCAA AGTACAGGCT TGACAACCGA AGTCCAGAAC 
CTGAAGCTAT CAGGGTGTGA AAGTCAGCAA CTTGACTATG CCAATAAGGA GAACATTGTC 
ACCTATTTAA CTGACAGACA AACCCCAGAA AAACTGCATA TACCAACGAT AGCAAAGAAC 
CTTATGTCTG AACTAGATGA AGACTGTGAG CTGAGCAGTA AAAAGGACTG CCTCAGTTCT 
AATTCTGTAT GTTCTGATGA GGATAGAGCT CTTAAAACTA CCTGTGTGGA TTCGGATTCA 
TCTTTTCCTG GAGTGTCTAT GATGGAGAGT TCACTGGAAA TTCAGGCCTT AGAACCAGAT 
AAAAGCATCA GAGACTACTC TTTTGAAGAA CCAAACACTG AAGATCTATT TGTGTTACCA 
AAATGCCAAG AAAATTCCTT GCCACAAGAT GACTGCCATG CTTGTATCCA AGACAGTAGC 
CAAGTGTCAG CTCATCCCTC AAAGGCGCCC AAAGCATTGA CCTCTAAAAT CAACGTCGTG 
GCTTTTCGAA GTTTTAACAG TCATATTAAC GCATCTACTA ATTCGGAACC ATCCAAAATC 
AGCATAACTT CTTTAGATGC TATGGACATT TCCTGTGATT ACAGTGGTTC ATACCCCATG 
GCTGTGAGCC CTACTGAGAA AGGAAGGCAC TACACGTCAC ATCAGACTCC AAATCAGGTC 
AAGTTGGGAA CATCATACAG AACTCCAAAG AGTGTGAGAA GAGGGGCAGC CCCAGTAGAT 
GACGGGCGAA TTCTAGGGAC TCCAGATTAC CTGGCACCTG AGCTCTTACT GGGTACAGCC 
CATGGTCCTG CAGTAGACTG GTGGGCTCTT GGAGTTTGCT TGTTCGAATT TCTGACAGGA 
ATTCCCCCTT TCAATGATGA AACACCACAA CAAGTATTCC AGAATATTTT AAAAAGAGAT 
ATCCCATGGC CAGAAGGCGA AGAAAAGCTA TCTGATAATG CTCAAAGTGC AATGGACATG 
CTTTTAACCA TTGATGATTC AAAGAGAGCT GGAATGAGAG AACTAAAACA GCATCCTCTC 
TTCAGTGAAG TGGACTGGGA AAATCTGCAG CATCAGACTA TGCCTTTCGT ACCCCAACCA 
GACGACGAAA CAGATACATC CTATTTTGAA GCCAGAAATA ATGCTCAACA TCTGACCATA 
TCTGGGTTTA GTCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mastl ( XM_006498239.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006498302.1 Mus musculus microtubule associated serine/threonine kinase-like (Mastl), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu29679
Clone ID Related Accession (Same CDS sequence)	XM_006498239.3 , XM_030251977.1
Accession Version	XM_006498239.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2370bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase greatwall isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006498239.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCAGG GTGACCCAAC TGCTTTGTTT GGAGAGGGGA TGTACTTGCA GCTGAATGCA 
GATGGGGCTG GAGCCAGACA TGACCCCGGA TCGATAATGG AGTATCTTAT TGGTGGAGAT 
GTCAAGTCTC TCCTACATAT ATATGGTTAT TTTGATGAAG AGATGGCTAT AAAATATATT 
TCTGAAGTAG CATTGGCTTT AGACTACCTG CACAGACATG GAATCATCCA TAGGGATTTG 
AAACCGGACA ATATGCTTAT TTCCAATGAG GGTCATATTA AACTGACAGA TTTTGGCCTT 
TCCAAAGTTA CTTTGAATAG AGACATCAAT ATGATGGATA TTCTCACAAC ACCATCAATG 
TCTAAACCTA AGCAAGATTA TTCAAGAACT CCAGGACAAG TCTTATCTCT CATCAGTTCT 
TTGGGATTTT TCACACCAGT TGGAGAAAAA GATCAAGACT CTGCAAACAT GTTTTCAGCC 
CCTAAGTCTG CTGCACAACT TTCTCGAGGA TTCATTTGTC CTATGTCTGT AGATCAGAAG 
GAGCCGACTT CTTATTCGAG CAAACTGCTA AAATCATGTT TTGAAACCCT TTCATCCAAT 
CCAGAAATAC CTGTGAAATG TCTGACTTCT AATCTACTCC AGTGTAGGAA AAGACTGGGT 
ACCTCAAGTA CTAGTAGCCA GTCTCATACC TTCGTATCCA GTGTGGAGTC AGAATGCCAC 
AGCAATCCCA AATGGGAAAG AGACTGCCAG AGTACCGAAT CATCAGGCTG TGCAATGAGC 
TGGAATGCAG TTGAAATGCT GTATGCAAAA TCTACAAGTG CCATCAAGAC AAAAACAGAA 
CTGGAATTAG CACTTTCTCC CATTCATGAC AGCAGTGCCA TTCCTGCTGC TGGAAGCAAC 
CAGGTGACCC TCCCTAGAAA ATGCTTCAGG GAAATCTCTT GGGAAGCAAG GGACCCAGAT 
AATGAGAACA TGACCATTGA CAAGGGACAG TCTGGTTTCT GTCAGTCCAG TCAGAGGTCT 
GTAAATTCTA GTGCTACATC TGAAGAACAC CTTGGTAAAA GAAATTATAA AAGAAATTTC 
CACTTGGTTG ACTCCAGTCC TTGTCAGGAA ATTATGCAAA GTAAAAAAAA CTGTACAGAG 
TATGAGGCCA ATAAGGAAAG GCAAGGCTGC CGTGCAAATC AAAGTACAGG CTTGACAACC 
GAAGTCCAGA ACCTGAAGCT ATCAGGGTGT GAAAGTCAGC AACTTGACTA TGCCAATAAG 
GAGAACATTG TCACCTATTT AACTGACAGA CAAACCCCAG AAAAACTGCA TATACCAACG 
ATAGCAAAGA ACCTTATGTC TGAACTAGAT GAAGACTGTG AGCTGAGCAG TAAAAAGGAC 
TGCCTCAGTT CTAATTCTGT ATGTTCTGAT GAGGATAGAG CTCTTAAAAC TACCTGTGTG 
GATTCGGATT CATCTTTTCC TGGAGTGTCT ATGATGGAGA GTTCACTGGA AATTCAGGCC 
TTAGAACCAG ATAAAAGCAT CAGAGACTAC TCTTTTGAAG AACCAAACAC TGAAGATCTA 
TTTGTGTTAC CAAAATGCCA AGAAAATTCC TTGCCACAAG ATGACTGCCA TGCTTGTATC 
CAAGACAGTA GCCAAGTGTC AGCTCATCCC TCAAAGGCGC CCAAAGCATT GACCTCTAAA 
ATCAACGTCG TGGCTTTTCG AAGTTTTAAC AGTCATATTA ACGCATCTAC TAATTCGGAA 
CCATCCAAAA TCAGCATAAC TTCTTTAGAT GCTATGGACA TTTCCTGTGA TTACAGTGGT 
TCATACCCCA TGGCTGTGAG CCCTACTGAG AAAGGAAGGC ACTACACGTC ACATCAGACT 
CCAAATCAGG TCAAGTTGGG AACATCATAC AGAACTCCAA AGAGTGTGAG AAGAGGGGCA 
GCCCCAGTAG ATGACGGGCG AATTCTAGGG ACTCCAGATT ACCTGGCACC TGAGCTCTTA 
CTGGGTACAG CCCATGGTCC TGCAGTAGAC TGGTGGGCTC TTGGAGTTTG CTTGTTCGAA 
TTTCTGACAG GAATTCCCCC TTTCAATGAT GAAACACCAC AACAAGTATT CCAGAATATT 
TTAAAAAGAG ATATCCCATG GCCAGAAGGC GAAGAAAAGC TATCTGATAA TGCTCAAAGT 
GCAATGGACA TGCTTTTAAC CATTGATGAT TCAAAGAGAG CTGGAATGAG AGAACTAAAA 
CAGCATCCTC TCTTCAGTGA AGTGGACTGG GAAAATCTGC AGCATCAGAC TATGCCTTTC 
GTACCCCAAC CAGACGACGA AACAGATACA TCCTATTTTG AAGCCAGAAA TAATGCTCAA 
CATCTGACCA TATCTGGGTT TAGTCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006498302.1
CDS	145..2514
Translation	MGQGDPTALFGEGMYLQLNADGAGARHDPGSIMEYLIGGDVKSLLHIYGYFDEEMAIKYISEVALALDYLHRHGIIHRDLKPDNMLISNEGHIKLTDFGLSKVTLNRDINMMDILTTPSMSKPKQDYSRTPGQVLSLISSLGFFTPVGEKDQDSANMFSAPKSAAQLSRGFICPMSVDQKEPTSYSSKLLKSCFETLSSNPEIPVKCLTSNLLQCRKRLGTSSTSSQSHTFVSSVESECHSNPKWERDCQSTESSGCAMSWNAVEMLYAKSTSAIKTKTELELALSPIHDSSAIPAAGSNQVTLPRKCFREISWEARDPDNENMTIDKGQSGFCQSSQRSVNSSATSEEHLGKRNYKRNFHLVDSSPCQEIMQSKKNCTEYEANKERQGCRANQSTGLTTEVQNLKLSGCESQQLDYANKENIVTYLTDRQTPEKLHIPTIAKNLMSELDEDCELSSKKDCLSSNSVCSDEDRALKTTCVDSDSSFPGVSMMESSLEIQALEPDKSIRDYSFEEPNTEDLFVLPKCQENSLPQDDCHACIQDSSQVSAHPSKAPKALTSKINVVAFRSFNSHINASTNSEPSKISITSLDAMDISCDYSGSYPMAVSPTEKGRHYTSHQTPNQVKLGTSYRTPKSVRRGAAPVDDGRILGTPDYLAPELLLGTAHGPAVDWWALGVCLFEFLTGIPPFNDETPQQVFQNILKRDIPWPEGEEKLSDNAQSAMDMLLTIDDSKRAGMRELKQHPLFSEVDWENLQHQTMPFVPQPDDETDTSYFEARNNAQHLTISGFSL

Target ORF information:

RefSeq Version	XM_006498239.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus microtubule associated serine/threonine kinase-like (Mastl), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498239.3

ORF Insert Sequence:

ATGGGTCAGG GTGACCCAAC TGCTTTGTTT GGAGAGGGGA TGTACTTGCA GCTGAATGCA 
GATGGGGCTG GAGCCAGACA TGACCCCGGA TCGATAATGG AGTATCTTAT TGGTGGAGAT 
GTCAAGTCTC TCCTACATAT ATATGGTTAT TTTGATGAAG AGATGGCTAT AAAATATATT 
TCTGAAGTAG CATTGGCTTT AGACTACCTG CACAGACATG GAATCATCCA TAGGGATTTG 
AAACCGGACA ATATGCTTAT TTCCAATGAG GGTCATATTA AACTGACAGA TTTTGGCCTT 
TCCAAAGTTA CTTTGAATAG AGACATCAAT ATGATGGATA TTCTCACAAC ACCATCAATG 
TCTAAACCTA AGCAAGATTA TTCAAGAACT CCAGGACAAG TCTTATCTCT CATCAGTTCT 
TTGGGATTTT TCACACCAGT TGGAGAAAAA GATCAAGACT CTGCAAACAT GTTTTCAGCC 
CCTAAGTCTG CTGCACAACT TTCTCGAGGA TTCATTTGTC CTATGTCTGT AGATCAGAAG 
GAGCCGACTT CTTATTCGAG CAAACTGCTA AAATCATGTT TTGAAACCCT TTCATCCAAT 
CCAGAAATAC CTGTGAAATG TCTGACTTCT AATCTACTCC AGTGTAGGAA AAGACTGGGT 
ACCTCAAGTA CTAGTAGCCA GTCTCATACC TTCGTATCCA GTGTGGAGTC AGAATGCCAC 
AGCAATCCCA AATGGGAAAG AGACTGCCAG AGTACCGAAT CATCAGGCTG TGCAATGAGC 
TGGAATGCAG TTGAAATGCT GTATGCAAAA TCTACAAGTG CCATCAAGAC AAAAACAGAA 
CTGGAATTAG CACTTTCTCC CATTCATGAC AGCAGTGCCA TTCCTGCTGC TGGAAGCAAC 
CAGGTGACCC TCCCTAGAAA ATGCTTCAGG GAAATCTCTT GGGAAGCAAG GGACCCAGAT 
AATGAGAACA TGACCATTGA CAAGGGACAG TCTGGTTTCT GTCAGTCCAG TCAGAGGTCT 
GTAAATTCTA GTGCTACATC TGAAGAACAC CTTGGTAAAA GAAATTATAA AAGAAATTTC 
CACTTGGTTG ACTCCAGTCC TTGTCAGGAA ATTATGCAAA GTAAAAAAAA CTGTACAGAG 
TATGAGGCCA ATAAGGAAAG GCAAGGCTGC CGTGCAAATC AAAGTACAGG CTTGACAACC 
GAAGTCCAGA ACCTGAAGCT ATCAGGGTGT GAAAGTCAGC AACTTGACTA TGCCAATAAG 
GAGAACATTG TCACCTATTT AACTGACAGA CAAACCCCAG AAAAACTGCA TATACCAACG 
ATAGCAAAGA ACCTTATGTC TGAACTAGAT GAAGACTGTG AGCTGAGCAG TAAAAAGGAC 
TGCCTCAGTT CTAATTCTGT ATGTTCTGAT GAGGATAGAG CTCTTAAAAC TACCTGTGTG 
GATTCGGATT CATCTTTTCC TGGAGTGTCT ATGATGGAGA GTTCACTGGA AATTCAGGCC 
TTAGAACCAG ATAAAAGCAT CAGAGACTAC TCTTTTGAAG AACCAAACAC TGAAGATCTA 
TTTGTGTTAC CAAAATGCCA AGAAAATTCC TTGCCACAAG ATGACTGCCA TGCTTGTATC 
CAAGACAGTA GCCAAGTGTC AGCTCATCCC TCAAAGGCGC CCAAAGCATT GACCTCTAAA 
ATCAACGTCG TGGCTTTTCG AAGTTTTAAC AGTCATATTA ACGCATCTAC TAATTCGGAA 
CCATCCAAAA TCAGCATAAC TTCTTTAGAT GCTATGGACA TTTCCTGTGA TTACAGTGGT 
TCATACCCCA TGGCTGTGAG CCCTACTGAG AAAGGAAGGC ACTACACGTC ACATCAGACT 
CCAAATCAGG TCAAGTTGGG AACATCATAC AGAACTCCAA AGAGTGTGAG AAGAGGGGCA 
GCCCCAGTAG ATGACGGGCG AATTCTAGGG ACTCCAGATT ACCTGGCACC TGAGCTCTTA 
CTGGGTACAG CCCATGGTCC TGCAGTAGAC TGGTGGGCTC TTGGAGTTTG CTTGTTCGAA 
TTTCTGACAG GAATTCCCCC TTTCAATGAT GAAACACCAC AACAAGTATT CCAGAATATT 
TTAAAAAGAG ATATCCCATG GCCAGAAGGC GAAGAAAAGC TATCTGATAA TGCTCAAAGT 
GCAATGGACA TGCTTTTAAC CATTGATGAT TCAAAGAGAG CTGGAATGAG AGAACTAAAA 
CAGCATCCTC TCTTCAGTGA AGTGGACTGG GAAAATCTGC AGCATCAGAC TATGCCTTTC 
GTACCCCAAC CAGACGACGA AACAGATACA TCCTATTTTG AAGCCAGAAA TAATGCTCAA 
CATCTGACCA TATCTGGGTT TAGTCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mastl ( XM_030251977.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030107837.1 Mus musculus microtubule associated serine/threonine kinase-like (Mastl), transcript variant X1, mRNA.

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CloneID	OMu29679
Clone ID Related Accession (Same CDS sequence)	XM_006498239.3 , XM_030251977.1
Accession Version	XM_030251977.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2370bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase greatwall isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCAGG GTGACCCAAC TGCTTTGTTT GGAGAGGGGA TGTACTTGCA GCTGAATGCA 
GATGGGGCTG GAGCCAGACA TGACCCCGGA TCGATAATGG AGTATCTTAT TGGTGGAGAT 
GTCAAGTCTC TCCTACATAT ATATGGTTAT TTTGATGAAG AGATGGCTAT AAAATATATT 
TCTGAAGTAG CATTGGCTTT AGACTACCTG CACAGACATG GAATCATCCA TAGGGATTTG 
AAACCGGACA ATATGCTTAT TTCCAATGAG GGTCATATTA AACTGACAGA TTTTGGCCTT 
TCCAAAGTTA CTTTGAATAG AGACATCAAT ATGATGGATA TTCTCACAAC ACCATCAATG 
TCTAAACCTA AGCAAGATTA TTCAAGAACT CCAGGACAAG TCTTATCTCT CATCAGTTCT 
TTGGGATTTT TCACACCAGT TGGAGAAAAA GATCAAGACT CTGCAAACAT GTTTTCAGCC 
CCTAAGTCTG CTGCACAACT TTCTCGAGGA TTCATTTGTC CTATGTCTGT AGATCAGAAG 
GAGCCGACTT CTTATTCGAG CAAACTGCTA AAATCATGTT TTGAAACCCT TTCATCCAAT 
CCAGAAATAC CTGTGAAATG TCTGACTTCT AATCTACTCC AGTGTAGGAA AAGACTGGGT 
ACCTCAAGTA CTAGTAGCCA GTCTCATACC TTCGTATCCA GTGTGGAGTC AGAATGCCAC 
AGCAATCCCA AATGGGAAAG AGACTGCCAG AGTACCGAAT CATCAGGCTG TGCAATGAGC 
TGGAATGCAG TTGAAATGCT GTATGCAAAA TCTACAAGTG CCATCAAGAC AAAAACAGAA 
CTGGAATTAG CACTTTCTCC CATTCATGAC AGCAGTGCCA TTCCTGCTGC TGGAAGCAAC 
CAGGTGACCC TCCCTAGAAA ATGCTTCAGG GAAATCTCTT GGGAAGCAAG GGACCCAGAT 
AATGAGAACA TGACCATTGA CAAGGGACAG TCTGGTTTCT GTCAGTCCAG TCAGAGGTCT 
GTAAATTCTA GTGCTACATC TGAAGAACAC CTTGGTAAAA GAAATTATAA AAGAAATTTC 
CACTTGGTTG ACTCCAGTCC TTGTCAGGAA ATTATGCAAA GTAAAAAAAA CTGTACAGAG 
TATGAGGCCA ATAAGGAAAG GCAAGGCTGC CGTGCAAATC AAAGTACAGG CTTGACAACC 
GAAGTCCAGA ACCTGAAGCT ATCAGGGTGT GAAAGTCAGC AACTTGACTA TGCCAATAAG 
GAGAACATTG TCACCTATTT AACTGACAGA CAAACCCCAG AAAAACTGCA TATACCAACG 
ATAGCAAAGA ACCTTATGTC TGAACTAGAT GAAGACTGTG AGCTGAGCAG TAAAAAGGAC 
TGCCTCAGTT CTAATTCTGT ATGTTCTGAT GAGGATAGAG CTCTTAAAAC TACCTGTGTG 
GATTCGGATT CATCTTTTCC TGGAGTGTCT ATGATGGAGA GTTCACTGGA AATTCAGGCC 
TTAGAACCAG ATAAAAGCAT CAGAGACTAC TCTTTTGAAG AACCAAACAC TGAAGATCTA 
TTTGTGTTAC CAAAATGCCA AGAAAATTCC TTGCCACAAG ATGACTGCCA TGCTTGTATC 
CAAGACAGTA GCCAAGTGTC AGCTCATCCC TCAAAGGCGC CCAAAGCATT GACCTCTAAA 
ATCAACGTCG TGGCTTTTCG AAGTTTTAAC AGTCATATTA ACGCATCTAC TAATTCGGAA 
CCATCCAAAA TCAGCATAAC TTCTTTAGAT GCTATGGACA TTTCCTGTGA TTACAGTGGT 
TCATACCCCA TGGCTGTGAG CCCTACTGAG AAAGGAAGGC ACTACACGTC ACATCAGACT 
CCAAATCAGG TCAAGTTGGG AACATCATAC AGAACTCCAA AGAGTGTGAG AAGAGGGGCA 
GCCCCAGTAG ATGACGGGCG AATTCTAGGG ACTCCAGATT ACCTGGCACC TGAGCTCTTA 
CTGGGTACAG CCCATGGTCC TGCAGTAGAC TGGTGGGCTC TTGGAGTTTG CTTGTTCGAA 
TTTCTGACAG GAATTCCCCC TTTCAATGAT GAAACACCAC AACAAGTATT CCAGAATATT 
TTAAAAAGAG ATATCCCATG GCCAGAAGGC GAAGAAAAGC TATCTGATAA TGCTCAAAGT 
GCAATGGACA TGCTTTTAAC CATTGATGAT TCAAAGAGAG CTGGAATGAG AGAACTAAAA 
CAGCATCCTC TCTTCAGTGA AGTGGACTGG GAAAATCTGC AGCATCAGAC TATGCCTTTC 
GTACCCCAAC CAGACGACGA AACAGATACA TCCTATTTTG AAGCCAGAAA TAATGCTCAA 
CATCTGACCA TATCTGGGTT TAGTCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030107837.1
CDS	385..2754
Translation	MGQGDPTALFGEGMYLQLNADGAGARHDPGSIMEYLIGGDVKSLLHIYGYFDEEMAIKYISEVALALDYLHRHGIIHRDLKPDNMLISNEGHIKLTDFGLSKVTLNRDINMMDILTTPSMSKPKQDYSRTPGQVLSLISSLGFFTPVGEKDQDSANMFSAPKSAAQLSRGFICPMSVDQKEPTSYSSKLLKSCFETLSSNPEIPVKCLTSNLLQCRKRLGTSSTSSQSHTFVSSVESECHSNPKWERDCQSTESSGCAMSWNAVEMLYAKSTSAIKTKTELELALSPIHDSSAIPAAGSNQVTLPRKCFREISWEARDPDNENMTIDKGQSGFCQSSQRSVNSSATSEEHLGKRNYKRNFHLVDSSPCQEIMQSKKNCTEYEANKERQGCRANQSTGLTTEVQNLKLSGCESQQLDYANKENIVTYLTDRQTPEKLHIPTIAKNLMSELDEDCELSSKKDCLSSNSVCSDEDRALKTTCVDSDSSFPGVSMMESSLEIQALEPDKSIRDYSFEEPNTEDLFVLPKCQENSLPQDDCHACIQDSSQVSAHPSKAPKALTSKINVVAFRSFNSHINASTNSEPSKISITSLDAMDISCDYSGSYPMAVSPTEKGRHYTSHQTPNQVKLGTSYRTPKSVRRGAAPVDDGRILGTPDYLAPELLLGTAHGPAVDWWALGVCLFEFLTGIPPFNDETPQQVFQNILKRDIPWPEGEEKLSDNAQSAMDMLLTIDDSKRAGMRELKQHPLFSEVDWENLQHQTMPFVPQPDDETDTSYFEARNNAQHLTISGFSL

Target ORF information:

RefSeq Version	XM_030251977.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus microtubule associated serine/threonine kinase-like (Mastl), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030251977.1

ORF Insert Sequence:

ATGGGTCAGG GTGACCCAAC TGCTTTGTTT GGAGAGGGGA TGTACTTGCA GCTGAATGCA 
GATGGGGCTG GAGCCAGACA TGACCCCGGA TCGATAATGG AGTATCTTAT TGGTGGAGAT 
GTCAAGTCTC TCCTACATAT ATATGGTTAT TTTGATGAAG AGATGGCTAT AAAATATATT 
TCTGAAGTAG CATTGGCTTT AGACTACCTG CACAGACATG GAATCATCCA TAGGGATTTG 
AAACCGGACA ATATGCTTAT TTCCAATGAG GGTCATATTA AACTGACAGA TTTTGGCCTT 
TCCAAAGTTA CTTTGAATAG AGACATCAAT ATGATGGATA TTCTCACAAC ACCATCAATG 
TCTAAACCTA AGCAAGATTA TTCAAGAACT CCAGGACAAG TCTTATCTCT CATCAGTTCT 
TTGGGATTTT TCACACCAGT TGGAGAAAAA GATCAAGACT CTGCAAACAT GTTTTCAGCC 
CCTAAGTCTG CTGCACAACT TTCTCGAGGA TTCATTTGTC CTATGTCTGT AGATCAGAAG 
GAGCCGACTT CTTATTCGAG CAAACTGCTA AAATCATGTT TTGAAACCCT TTCATCCAAT 
CCAGAAATAC CTGTGAAATG TCTGACTTCT AATCTACTCC AGTGTAGGAA AAGACTGGGT 
ACCTCAAGTA CTAGTAGCCA GTCTCATACC TTCGTATCCA GTGTGGAGTC AGAATGCCAC 
AGCAATCCCA AATGGGAAAG AGACTGCCAG AGTACCGAAT CATCAGGCTG TGCAATGAGC 
TGGAATGCAG TTGAAATGCT GTATGCAAAA TCTACAAGTG CCATCAAGAC AAAAACAGAA 
CTGGAATTAG CACTTTCTCC CATTCATGAC AGCAGTGCCA TTCCTGCTGC TGGAAGCAAC 
CAGGTGACCC TCCCTAGAAA ATGCTTCAGG GAAATCTCTT GGGAAGCAAG GGACCCAGAT 
AATGAGAACA TGACCATTGA CAAGGGACAG TCTGGTTTCT GTCAGTCCAG TCAGAGGTCT 
GTAAATTCTA GTGCTACATC TGAAGAACAC CTTGGTAAAA GAAATTATAA AAGAAATTTC 
CACTTGGTTG ACTCCAGTCC TTGTCAGGAA ATTATGCAAA GTAAAAAAAA CTGTACAGAG 
TATGAGGCCA ATAAGGAAAG GCAAGGCTGC CGTGCAAATC AAAGTACAGG CTTGACAACC 
GAAGTCCAGA ACCTGAAGCT ATCAGGGTGT GAAAGTCAGC AACTTGACTA TGCCAATAAG 
GAGAACATTG TCACCTATTT AACTGACAGA CAAACCCCAG AAAAACTGCA TATACCAACG 
ATAGCAAAGA ACCTTATGTC TGAACTAGAT GAAGACTGTG AGCTGAGCAG TAAAAAGGAC 
TGCCTCAGTT CTAATTCTGT ATGTTCTGAT GAGGATAGAG CTCTTAAAAC TACCTGTGTG 
GATTCGGATT CATCTTTTCC TGGAGTGTCT ATGATGGAGA GTTCACTGGA AATTCAGGCC 
TTAGAACCAG ATAAAAGCAT CAGAGACTAC TCTTTTGAAG AACCAAACAC TGAAGATCTA 
TTTGTGTTAC CAAAATGCCA AGAAAATTCC TTGCCACAAG ATGACTGCCA TGCTTGTATC 
CAAGACAGTA GCCAAGTGTC AGCTCATCCC TCAAAGGCGC CCAAAGCATT GACCTCTAAA 
ATCAACGTCG TGGCTTTTCG AAGTTTTAAC AGTCATATTA ACGCATCTAC TAATTCGGAA 
CCATCCAAAA TCAGCATAAC TTCTTTAGAT GCTATGGACA TTTCCTGTGA TTACAGTGGT 
TCATACCCCA TGGCTGTGAG CCCTACTGAG AAAGGAAGGC ACTACACGTC ACATCAGACT 
CCAAATCAGG TCAAGTTGGG AACATCATAC AGAACTCCAA AGAGTGTGAG AAGAGGGGCA 
GCCCCAGTAG ATGACGGGCG AATTCTAGGG ACTCCAGATT ACCTGGCACC TGAGCTCTTA 
CTGGGTACAG CCCATGGTCC TGCAGTAGAC TGGTGGGCTC TTGGAGTTTG CTTGTTCGAA 
TTTCTGACAG GAATTCCCCC TTTCAATGAT GAAACACCAC AACAAGTATT CCAGAATATT 
TTAAAAAGAG ATATCCCATG GCCAGAAGGC GAAGAAAAGC TATCTGATAA TGCTCAAAGT 
GCAATGGACA TGCTTTTAAC CATTGATGAT TCAAAGAGAG CTGGAATGAG AGAACTAAAA 
CAGCATCCTC TCTTCAGTGA AGTGGACTGG GAAAATCTGC AGCATCAGAC TATGCCTTTC 
GTACCCCAAC CAGACGACGA AACAGATACA TCCTATTTTG AAGCCAGAAA TAATGCTCAA 
CATCTGACCA TATCTGGGTT TAGTCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mastl ( XM_017319214.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017174703.1 Mus musculus microtubule associated serine/threonine kinase-like (Mastl), transcript variant X2, mRNA.

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CloneID	OMu80391
Clone ID Related Accession (Same CDS sequence)	XM_017319214.1
Accession Version	XM_017319214.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase greatwall isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATAGCAG ACATCAATAT GATGGATATT CTCACAACAC CATCAATGTC TAAACCTAAG 
CAAGATTATT CAAGAACTCC AGGACAAGTC TTATCTCTCA TCAGTTCTTT GGGATTTTTC 
ACACCAGTTG GAGAAAAAGA TCAAGACTCT GCAAACATGT TTTCAGCCCC TAAGTCTGCT 
GCACAACTTT CTCGAGGATT CATTTGTCCT ATGTCTGTAG ATCAGAAGGA GCCGACTTCT 
TATTCGAGCA AACTGCTAAA ATCATGTTTT GAAACCCTTT CATCCAATCC AGAAATACCT 
GTGAAATGTC TGACTTCTAA TCTACTCCAG TGTAGGAAAA GACTGGGTAC CTCAAGTACT 
AGTAGCCAGT CTCATACCTT CGTATCCAGT GTGGAGTCAG AATGCCACAG CAATCCCAAA 
TGGGAAAGAG ACTGCCAGAG TACCGAATCA TCAGGCTGTG CAATGAGCTG GAATGCAGTT 
GAAATGCTGT ATGCAAAATC TACAAGTGCC ATCAAGACAA AAACAGAACT GGAATTAGCA 
CTTTCTCCCA TTCATGACAG CAGTGCCATT CCTGCTGCTG GAAGCAACCA GGTGACCCTC 
CCTAGAAAAT GCTTCAGGGA AATCTCTTGG GAAGCAAGGG ACCCAGATAA TGAGAACATG 
ACCATTGACA AGGGACAGTC TGGTTTCTGT CAGTCCAGTC AGAGGTCTGT AAATTCTAGT 
GCTACATCTG AAGAACACCT TGGTAAAAGA AATTATAAAA GAAATTTCCA CTTGGTTGAC 
TCCAGTCCTT GTCAGGAAAT TATGCAAAGT AAAAAAAACT GTACAGAGTA TGAGGCCAAT 
AAGGAAAGGC AAGGCTGCCG TGCAAATCAA AGTACAGGCT TGACAACCGA AGTCCAGAAC 
CTGAAGCTAT CAGGGTGTGA AAGTCAGCAA CTTGACTATG CCAATAAGGA GAACATTGTC 
ACCTATTTAA CTGACAGACA AACCCCAGAA AAACTGCATA TACCAACGAT AGCAAAGAAC 
CTTATGTCTG AACTAGATGA AGACTGTGAG CTGAGCAGTA AAAAGGACTG CCTCAGTTCT 
AATTCTGTAT GTTCTGATGA GGATAGAGCT CTTAAAACTA CCTGTGTGGA TTCGGATTCA 
TCTTTTCCTG GAGTGTCTAT GATGGAGAGT TCACTGGAAA TTCAGGCCTT AGAACCAGAT 
AAAAGCATCA GAGACTACTC TTTTGAAGAA CCAAACACTG AAGATCTATT TGTGTTACCA 
AAATGCCAAG AAAATTCCTT GCCACAAGAT GACTGCCATG CTTGTATCCA AGACAGTAGC 
CAAGTGTCAG CTCATCCCTC AAAGGCGCCC AAAGCATTGA CCTCTAAAAT CAACGTCGTG 
GCTTTTCGAA GTTTTAACAG TCATATTAAC GCATCTACTA ATTCGGAACC ATCCAAAATC 
AGCATAACTT CTTTAGATGC TATGGACATT TCCTGTGATT ACAGTGGTTC ATACCCCATG 
GCTGTGAGCC CTACTGAGAA AGGAAGGCAC TACACGTCAC ATCAGACTCC AAATCAGGTC 
AAGTTGGGAA CATCATACAG AACTCCAAAG AGTGTGAGAA GAGGGGCAGC CCCAGTAGAT 
GACGGGCGAA TTCTAGGGAC TCCAGATTAC CTGGCACCTG AGCTCTTACT GGGTACAGCC 
CATGGTCCTG CAGTAGACTG GTGGGCTCTT GGAGTTTGCT TGTTCGAATT TCTGACAGGA 
ATTCCCCCTT TCAATGATGA AACACCACAA CAAGTATTCC AGAATATTTT AAAAAGAGAT 
ATCCCATGGC CAGAAGGCGA AGAAAAGCTA TCTGATAATG CTCAAAGTGC AATGGACATG 
CTTTTAACCA TTGATGATTC AAAGAGAGCT GGAATGAGAG AACTAAAACA GCATCCTCTC 
TTCAGTGAAG TGGACTGGGA AAATCTGCAG CATCAGACTA TGCCTTTCGT ACCCCAACCA 
GACGACGAAA CAGATACATC CTATTTTGAA GCCAGAAATA ATGCTCAACA TCTGACCATA 
TCTGGGTTTA GTCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017174703.1
CDS	68..2125
Translation	MIADINMMDILTTPSMSKPKQDYSRTPGQVLSLISSLGFFTPVGEKDQDSANMFSAPKSAAQLSRGFICPMSVDQKEPTSYSSKLLKSCFETLSSNPEIPVKCLTSNLLQCRKRLGTSSTSSQSHTFVSSVESECHSNPKWERDCQSTESSGCAMSWNAVEMLYAKSTSAIKTKTELELALSPIHDSSAIPAAGSNQVTLPRKCFREISWEARDPDNENMTIDKGQSGFCQSSQRSVNSSATSEEHLGKRNYKRNFHLVDSSPCQEIMQSKKNCTEYEANKERQGCRANQSTGLTTEVQNLKLSGCESQQLDYANKENIVTYLTDRQTPEKLHIPTIAKNLMSELDEDCELSSKKDCLSSNSVCSDEDRALKTTCVDSDSSFPGVSMMESSLEIQALEPDKSIRDYSFEEPNTEDLFVLPKCQENSLPQDDCHACIQDSSQVSAHPSKAPKALTSKINVVAFRSFNSHINASTNSEPSKISITSLDAMDISCDYSGSYPMAVSPTEKGRHYTSHQTPNQVKLGTSYRTPKSVRRGAAPVDDGRILGTPDYLAPELLLGTAHGPAVDWWALGVCLFEFLTGIPPFNDETPQQVFQNILKRDIPWPEGEEKLSDNAQSAMDMLLTIDDSKRAGMRELKQHPLFSEVDWENLQHQTMPFVPQPDDETDTSYFEARNNAQHLTISGFSL

Target ORF information:

RefSeq Version	XM_017319214.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus microtubule associated serine/threonine kinase-like (Mastl), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319214.1

ORF Insert Sequence:

ATGATAGCAG ACATCAATAT GATGGATATT CTCACAACAC CATCAATGTC TAAACCTAAG 
CAAGATTATT CAAGAACTCC AGGACAAGTC TTATCTCTCA TCAGTTCTTT GGGATTTTTC 
ACACCAGTTG GAGAAAAAGA TCAAGACTCT GCAAACATGT TTTCAGCCCC TAAGTCTGCT 
GCACAACTTT CTCGAGGATT CATTTGTCCT ATGTCTGTAG ATCAGAAGGA GCCGACTTCT 
TATTCGAGCA AACTGCTAAA ATCATGTTTT GAAACCCTTT CATCCAATCC AGAAATACCT 
GTGAAATGTC TGACTTCTAA TCTACTCCAG TGTAGGAAAA GACTGGGTAC CTCAAGTACT 
AGTAGCCAGT CTCATACCTT CGTATCCAGT GTGGAGTCAG AATGCCACAG CAATCCCAAA 
TGGGAAAGAG ACTGCCAGAG TACCGAATCA TCAGGCTGTG CAATGAGCTG GAATGCAGTT 
GAAATGCTGT ATGCAAAATC TACAAGTGCC ATCAAGACAA AAACAGAACT GGAATTAGCA 
CTTTCTCCCA TTCATGACAG CAGTGCCATT CCTGCTGCTG GAAGCAACCA GGTGACCCTC 
CCTAGAAAAT GCTTCAGGGA AATCTCTTGG GAAGCAAGGG ACCCAGATAA TGAGAACATG 
ACCATTGACA AGGGACAGTC TGGTTTCTGT CAGTCCAGTC AGAGGTCTGT AAATTCTAGT 
GCTACATCTG AAGAACACCT TGGTAAAAGA AATTATAAAA GAAATTTCCA CTTGGTTGAC 
TCCAGTCCTT GTCAGGAAAT TATGCAAAGT AAAAAAAACT GTACAGAGTA TGAGGCCAAT 
AAGGAAAGGC AAGGCTGCCG TGCAAATCAA AGTACAGGCT TGACAACCGA AGTCCAGAAC 
CTGAAGCTAT CAGGGTGTGA AAGTCAGCAA CTTGACTATG CCAATAAGGA GAACATTGTC 
ACCTATTTAA CTGACAGACA AACCCCAGAA AAACTGCATA TACCAACGAT AGCAAAGAAC 
CTTATGTCTG AACTAGATGA AGACTGTGAG CTGAGCAGTA AAAAGGACTG CCTCAGTTCT 
AATTCTGTAT GTTCTGATGA GGATAGAGCT CTTAAAACTA CCTGTGTGGA TTCGGATTCA 
TCTTTTCCTG GAGTGTCTAT GATGGAGAGT TCACTGGAAA TTCAGGCCTT AGAACCAGAT 
AAAAGCATCA GAGACTACTC TTTTGAAGAA CCAAACACTG AAGATCTATT TGTGTTACCA 
AAATGCCAAG AAAATTCCTT GCCACAAGAT GACTGCCATG CTTGTATCCA AGACAGTAGC 
CAAGTGTCAG CTCATCCCTC AAAGGCGCCC AAAGCATTGA CCTCTAAAAT CAACGTCGTG 
GCTTTTCGAA GTTTTAACAG TCATATTAAC GCATCTACTA ATTCGGAACC ATCCAAAATC 
AGCATAACTT CTTTAGATGC TATGGACATT TCCTGTGATT ACAGTGGTTC ATACCCCATG 
GCTGTGAGCC CTACTGAGAA AGGAAGGCAC TACACGTCAC ATCAGACTCC AAATCAGGTC 
AAGTTGGGAA CATCATACAG AACTCCAAAG AGTGTGAGAA GAGGGGCAGC CCCAGTAGAT 
GACGGGCGAA TTCTAGGGAC TCCAGATTAC CTGGCACCTG AGCTCTTACT GGGTACAGCC 
CATGGTCCTG CAGTAGACTG GTGGGCTCTT GGAGTTTGCT TGTTCGAATT TCTGACAGGA 
ATTCCCCCTT TCAATGATGA AACACCACAA CAAGTATTCC AGAATATTTT AAAAAGAGAT 
ATCCCATGGC CAGAAGGCGA AGAAAAGCTA TCTGATAATG CTCAAAGTGC AATGGACATG 
CTTTTAACCA TTGATGATTC AAAGAGAGCT GGAATGAGAG AACTAAAACA GCATCCTCTC 
TTCAGTGAAG TGGACTGGGA AAATCTGCAG CATCAGACTA TGCCTTTCGT ACCCCAACCA 
GACGACGAAA CAGATACATC CTATTTTGAA GCCAGAAATA ATGCTCAACA TCTGACCATA 
TCTGGGTTTA GTCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Loss of the Greatwall Kinase Weakens the Spindle Assembly Checkpoint.
PLoS genetics12(9)e1006310(2016 Sep)
Diril MK,Bisteau X,Kitagawa M,Caldez MJ,Wee S,Gunaratne J,Lee SH,Kaldis P

Mastl is required for timely activation of APC/C in meiosis I and Cdk1 reactivation in meiosis II.
The Journal of cell biology206(7)843-53(2014 Sep)
Adhikari D,Diril MK,Busayavalasa K,Risal S,Nakagawa S,Lindkvist R,Shen Y,Coppola V,Tessarollo L,Kudo NR,Kaldis P,Liu K

Greatwall is essential to prevent mitotic collapse after nuclear envelope breakdown in mammals.
Proceedings of the National Academy of Sciences of the United States of America110(43)17374-9(2013 Oct)
?lvarez-Fern?ndez M,S?nchez-Mart?nez R,Sanz-Castillo B,Gan PP,Sanz-Flores M,Trakala M,Ruiz-Torres M,Lorca T,Castro A,Malumbres M

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Mastl cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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