Fbxo25 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Fbxo25
Entrez Gene ID	66822
Full Name	F-box protein 25
Synonyms	9130015I06Rik,AI649137,Fbx25
General protein information	Preferred Names F-box protein 25 Names F-box only protein 25
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8\|8 A1.1

mRNA and Protein(s)

mRNA	Protein	Name
NM_025785.2	NP_080061.1	F-box only protein 25 isforom 2
NM_001347316.1	NP_001334245.1	F-box only protein 25 isoform 1
NM_001359061.1	NP_001345990.1	F-box only protein 25 isoform 3
XM_030243729.1	XP_030099589.1	F-box only protein 25 isoform X1
XM_006508854.3	XP_006508917.1	F-box only protein 25 isoform X1
NM_025785.3	NP_080061.1	F-box only protein 25 isoform 2

Pathways from BioSystems

Homologies

	FBXO25	XP_001095258.2
Bos taurus (cattle)	FBXO25	NP_001069679.1
Xenopus tropicalis (tropical clawed frog)	fbxo25	NP_001025702.1
Gallus gallus (chicken)	FBXO25	XP_004940546.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012127	XP_320404.2
Caenorhabditis elegans (roundworm)	mfb-1	NP_492376.1
Homo sapiens (human)	FBXO25	NP_904357.1
Canis lupus familiaris (dog)	FBXO25	XP_003433395.1
Mus musculus (house mouse)	Fbxo25	NP_080061.1
Rattus norvegicus (Norway rat)	Fbxo25	NP_001014261.1
Danio rerio (zebrafish)	fbxo25	NP_991287.1
Pan troglodytes (chimpanzee)	FBXO25	XP_001142756.1
Drosophila melanogaster (fruit fly)	CG11658	NP_996058.1

Gene Ontology
Bibliography

Related articles in PubMed

Fbxo25 controls Tbx5 and Nkx2-5 transcriptional activity to regulate cardiomyocyte development.
Jeong HS, Jung ES, Sim YJ, Kim SJ, Jang JW, Hong KS, Lee WY, Chung HM, Park KT, Jung YS, Kim CH, Kim KS
Biochimica et biophysica acta1849(6)709-21(2015 Jun)

A cluster of cooperating tumor-suppressor gene candidates in chromosomal deletions.
Xue W, Kitzing T, Roessler S, Zuber J, Krasnitz A, Schultz N, Revill K, Weissmueller S, Rappaport AR, Simon J, Zhang J, Luo W, Hicks J, Zender L, Wang XW, Powers S, Wigler M, Lowe SW
Proceedings of the National Academy of Sciences of the United States of America109(21)8212-7(2012 May)

A novel Fbxo25 acts as an E3 ligase for destructing cardiac specific transcription factors.
Jang JW, Lee WY, Lee JH, Moon SH, Kim CH, Chung HM
Biochemical and biophysical research communications410(2)183-8(2011 Jul)

FBXO25-associated nuclear domains: a novel subnuclear structure.
Manfiolli AO, Maragno AL, Baqui MM, Yokoo S, Teixeira FR, Oliveira EB, Gomes MD
Molecular biology of the cell19(5)1848-61(2008 May)

FBXO25, an F-box protein homologue of atrogin-1, is not induced in atrophying muscle.
Maragno AL, Baqui MM, Gomes MD
Biochimica et biophysica acta1760(6)966-72(2006 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

our findings reveal a novel mechanism for regulation of SCFFbox25-dependent Nkx2-5 and Tbx5 ubiquitination in cardiac development and provide a new insight into the regulatory mechanism of Nkx2-5 and Tbx5 transcriptional activity.
Title: Fbxo25 controls Tbx5 and Nkx2-5 transcriptional activity to regulate cardiomyocyte development.

Altogether, Fbxo25 acts as an ubiquitin E3 ligase to target cardiac transcription factors including Nkx2-5, Isl1, and Hand1, indicating that cardiac protein homeostasis through Fbxo25 has a pivotal impact on cardiac development.
Title: A novel Fbxo25 acts as an E3 ligase for destructing cardiac specific transcription factors.

The cellular distribution of FBXO25 and its colocalization with some nuclear proteins is investigated by using immunochemical and biochemical approaches.
Title: FBXO25-associated nuclear domains: a novel subnuclear structure.

FBXO25 is a novel F-box protein analogous to atrogin-1, which is not involved in muscle atrophy.
Title: FBXO25, an F-box protein homologue of atrogin-1, is not induced in atrophying muscle.

mFbx25 shows predominantly neuronal expression and in adult mouse brain, expression is confined to the hippocampus, the cerebral cortex and the Purkinje cell layer
Title: Characterization of FBX25, encoding a novel brain-expressed F-box protein.

The following Fbxo25 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Fbxo25 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu40443	NM_001359061.1 Latest version!	Mus musculus F-box protein 25 (Fbxo25), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu40444	NM_001347316.1 Latest version!	Mus musculus F-box protein 25 (Fbxo25), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu40443	XM_006508854.3 Latest version!	Mus musculus F-box protein 25 (Fbxo25), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu01027	NM_025785.3 Latest version!	Mus musculus F-box protein 25 (Fbxo25), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
OMu89928	XM_030243729.1 Latest version!	Mus musculus F-box protein 25 (Fbxo25), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OMu01027	NM_025785.2	Mus musculus F-box protein 25 (Fbxo25), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Fbxo25 ( NM_001359061.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001345990.1 Mus musculus F-box protein 25 (Fbxo25), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu40443
Clone ID Related Accession (Same CDS sequence)	NM_001359061.1 , XM_006508854.3
Accession Version	NM_001359061.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1101bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-25
Organism	Mus musculus(house mouse)
Product	F-box only protein 25 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC139186.4. On Dec 19, 2017 this sequence version replaced XM_006508854.3. Transcript Variant: This variant (3) represents the longest transcript and encodes the longest isoform (3). ##Evidence-Data-START## Transcript exon combination :: SRR1660819.41505.1, SRR1660815.137902.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END##

ATGCCGTTTC TGGGTCAGGA CTGGAGGTCC CCTGGATGGA GCTGGATTAA AACCGAGGAT 
GGGTGGAAAA GGTGTGACCC CTGTAGCCAC GAGCTCAGGA GCGAGGATAG CCAGTACACC 
ATCAACCACA GCATTATCTT AAATAGTGGT GAAGAAGAAA TATTCAACAA TGAGTGTGAA 
TACGCAGCCA AAAAGAGGAA GAAGGAACAT TTTGGAAATG ACACAGCTGC TCACAGTTTT 
TATCGTGAAA AATGGATCTA CGTCCATAAG GAAAGCACAA AGGAAAGGCA TGGCTACTGC 
ACCCTGGGAG AAGCCTTCAA CCGGCTAGAC TTCTCAAGTG CCATTCAGGA CATCAGGAGG 
TTCACTTACG TGGTCAAACT ACTGCAGCTA ATTGCGAAGT CCCAGTTAAC CTCACTGAGT 
GGAGTGGCAC AGAAGAACTA CTTCAACATT TTGGATAAAA TCGTCCAAAA GGTTCTTGAT 
GACCATCAGA ACCCTCGCCT GATCAAAGGC CTCCTCCAGG ACCTGAGCTC CACCCTTGGC 
ATCCTGGTGA GAGGGGTGGG GAAGTCAGTG CTAGTCGGGA ACATCAACAT CTGGATCTGC 
CGCCTGGAGA CCGTCCTCAG CTGGCAGCAG CAGCTGCAGA ACCTCCAGGT GACAAAGCAG 
GTAAACACTG GCCTCACACT CAGTGACCTG CCCCTGCACA TGCTGAACAA CATCCTGTAC 
CGTTTCTCAG ATGGCTGGGA CATTGTCACC CTGGGCCAGG TAACACCAAC CCTGTACATG 
CTCAGTGAAG ACAGGCGGCT GTGGAAGCGA CTGTGTCAGT ACCACTTTGC TGAGCAGCAG 
TTTTGTAGAC ATTTGATCCT TTCAGAAAAA GGTCATATCG AGTGGAAGCT GATGTATTTC 
ACGCTTCAGA AGTATTACCC GACCAAAGAG CAGTACGGGG ACACGCTACA CTTCTGCCGC 
CACTGCAGCA TCCTCTTCTG GAAGGACTAC CACCTTGCTT GGTTGTTGAA GGACTCGGGA 
CACCCCTGCA CCGCAGCTGA CCCCGACAGC TGCTTCACTC CTGTGTCTCC GGAGCACTTC 
ATTGACCTCT TCAAGTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001345990.1
CDS	133..1233
Translation	MPFLGQDWRSPGWSWIKTEDGWKRCDPCSHELRSEDSQYTINHSIILNSGEEEIFNNECEYAAKKRKKEHFGNDTAAHSFYREKWIYVHKESTKERHGYCTLGEAFNRLDFSSAIQDIRRFTYVVKLLQLIAKSQLTSLSGVAQKNYFNILDKIVQKVLDDHQNPRLIKGLLQDLSSTLGILVRGVGKSVLVGNINIWICRLETVLSWQQQLQNLQVTKQVNTGLTLSDLPLHMLNNILYRFSDGWDIVTLGQVTPTLYMLSEDRRLWKRLCQYHFAEQQFCRHLILSEKGHIEWKLMYFTLQKYYPTKEQYGDTLHFCRHCSILFWKDYHLAWLLKDSGHPCTAADPDSCFTPVSPEHFIDLFKF

Target ORF information:

RefSeq Version	NM_001359061.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus F-box protein 25 (Fbxo25), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001359061.1

ORF Insert Sequence:

ATGCCGTTTC TGGGTCAGGA CTGGAGGTCC CCTGGATGGA GCTGGATTAA AACCGAGGAT 
GGGTGGAAAA GGTGTGACCC CTGTAGCCAC GAGCTCAGGA GCGAGGATAG CCAGTACACC 
ATCAACCACA GCATTATCTT AAATAGTGGT GAAGAAGAAA TATTCAACAA TGAGTGTGAA 
TACGCAGCCA AAAAGAGGAA GAAGGAACAT TTTGGAAATG ACACAGCTGC TCACAGTTTT 
TATCGTGAAA AATGGATCTA CGTCCATAAG GAAAGCACAA AGGAAAGGCA TGGCTACTGC 
ACCCTGGGAG AAGCCTTCAA CCGGCTAGAC TTCTCAAGTG CCATTCAGGA CATCAGGAGG 
TTCACTTACG TGGTCAAACT ACTGCAGCTA ATTGCGAAGT CCCAGTTAAC CTCACTGAGT 
GGAGTGGCAC AGAAGAACTA CTTCAACATT TTGGATAAAA TCGTCCAAAA GGTTCTTGAT 
GACCATCAGA ACCCTCGCCT GATCAAAGGC CTCCTCCAGG ACCTGAGCTC CACCCTTGGC 
ATCCTGGTGA GAGGGGTGGG GAAGTCAGTG CTAGTCGGGA ACATCAACAT CTGGATCTGC 
CGCCTGGAGA CCGTCCTCAG CTGGCAGCAG CAGCTGCAGA ACCTCCAGGT GACAAAGCAG 
GTAAACACTG GCCTCACACT CAGTGACCTG CCCCTGCACA TGCTGAACAA CATCCTGTAC 
CGTTTCTCAG ATGGCTGGGA CATTGTCACC CTGGGCCAGG TAACACCAAC CCTGTACATG 
CTCAGTGAAG ACAGGCGGCT GTGGAAGCGA CTGTGTCAGT ACCACTTTGC TGAGCAGCAG 
TTTTGTAGAC ATTTGATCCT TTCAGAAAAA GGTCATATCG AGTGGAAGCT GATGTATTTC 
ACGCTTCAGA AGTATTACCC GACCAAAGAG CAGTACGGGG ACACGCTACA CTTCTGCCGC 
CACTGCAGCA TCCTCTTCTG GAAGGACTAC CACCTTGCTT GGTTGTTGAA GGACTCGGGA 
CACCCCTGCA CCGCAGCTGA CCCCGACAGC TGCTTCACTC CTGTGTCTCC GGAGCACTTC 
ATTGACCTCT TCAAGTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fbxo25 ( NM_001347316.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001334245.1 Mus musculus F-box protein 25 (Fbxo25), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu40444
Clone ID Related Accession (Same CDS sequence)	NM_001347316.1
Accession Version	NM_001347316.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1098bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Mus musculus(house mouse)
Product	F-box only protein 25 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ147979.1, AK134722.1 and AC139186.4. On Nov 19, 2016 this sequence version replaced XM_006508855.2. Transcript Variant: This variant (1) uses and alternate in-frame splice site, compared to variant 3. The encoded isoform (1) is shorter than isoform 3. ##Evidence-Data-START## Transcript exon combination :: AK134722.1, SRR1660819.167038.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END##

ATGCCGTTTC TGGGTCAGGA CTGGAGGTCC CCTGGATGGA GCTGGATTAA AACCGAGGAT 
GGGTGGAAAA GGTGTGACCC CTGTAGCCAC GAGCTCAGGA GCGAGGATAG CCAGTACACC 
ATCAACCACA GCATTATCTT AAATAGTGGT GAAGAAGAAA TATTCAACAA TGAGTGTGAA 
TACGCAGCCA AAAAGAGGAA GAAGGAACAT TTTGGAAATG ACACAGCTGC TCACAGTTTT 
TATCGTGAAA AATGGATCTA CGTCCATAAG GAAAGCACAA AGGAAAGGCA TGGCTACTGC 
ACCCTGGGAG AAGCCTTCAA CCGGCTAGAC TTCTCAAGTG CCATTCAGGA CATCAGGAGG 
TTCACTTACG TGGTCAAACT ACTGCAGCTA ATTGCGAAGT CCCAGTTAAC CTCACTGAGT 
GGAGTGGCAC AGAAGAACTA CTTCAACATT TTGGATAAAA TCGTCCAAAA GGTTCTTGAT 
GACCATCAGA ACCCTCGCCT GATCAAAGGC CTCCTCCAGG ACCTGAGCTC CACCCTTGGC 
ATCCTGGTGA GAGGGGTGGG GAAGTCAGTG CTAGTCGGGA ACATCAACAT CTGGATCTGC 
CGCCTGGAGA CCGTCCTCAG CTGGCAGCAG CAGCTGCAGA ACCTCCAGGT GACAAAGGTA 
AACACTGGCC TCACACTCAG TGACCTGCCC CTGCACATGC TGAACAACAT CCTGTACCGT 
TTCTCAGATG GCTGGGACAT TGTCACCCTG GGCCAGGTAA CACCAACCCT GTACATGCTC 
AGTGAAGACA GGCGGCTGTG GAAGCGACTG TGTCAGTACC ACTTTGCTGA GCAGCAGTTT 
TGTAGACATT TGATCCTTTC AGAAAAAGGT CATATCGAGT GGAAGCTGAT GTATTTCACG 
CTTCAGAAGT ATTACCCGAC CAAAGAGCAG TACGGGGACA CGCTACACTT CTGCCGCCAC 
TGCAGCATCC TCTTCTGGAA GGACTACCAC CTTGCTTGGT TGTTGAAGGA CTCGGGACAC 
CCCTGCACCG CAGCTGACCC CGACAGCTGC TTCACTCCTG TGTCTCCGGA GCACTTCATT 
GACCTCTTCA AGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001334245.1
CDS	133..1230
Translation	MPFLGQDWRSPGWSWIKTEDGWKRCDPCSHELRSEDSQYTINHSIILNSGEEEIFNNECEYAAKKRKKEHFGNDTAAHSFYREKWIYVHKESTKERHGYCTLGEAFNRLDFSSAIQDIRRFTYVVKLLQLIAKSQLTSLSGVAQKNYFNILDKIVQKVLDDHQNPRLIKGLLQDLSSTLGILVRGVGKSVLVGNINIWICRLETVLSWQQQLQNLQVTKVNTGLTLSDLPLHMLNNILYRFSDGWDIVTLGQVTPTLYMLSEDRRLWKRLCQYHFAEQQFCRHLILSEKGHIEWKLMYFTLQKYYPTKEQYGDTLHFCRHCSILFWKDYHLAWLLKDSGHPCTAADPDSCFTPVSPEHFIDLFKF

Target ORF information:

RefSeq Version	NM_001347316.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus F-box protein 25 (Fbxo25), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001347316.1

ORF Insert Sequence:

ATGCCGTTTC TGGGTCAGGA CTGGAGGTCC CCTGGATGGA GCTGGATTAA AACCGAGGAT 
GGGTGGAAAA GGTGTGACCC CTGTAGCCAC GAGCTCAGGA GCGAGGATAG CCAGTACACC 
ATCAACCACA GCATTATCTT AAATAGTGGT GAAGAAGAAA TATTCAACAA TGAGTGTGAA 
TACGCAGCCA AAAAGAGGAA GAAGGAACAT TTTGGAAATG ACACAGCTGC TCACAGTTTT 
TATCGTGAAA AATGGATCTA CGTCCATAAG GAAAGCACAA AGGAAAGGCA TGGCTACTGC 
ACCCTGGGAG AAGCCTTCAA CCGGCTAGAC TTCTCAAGTG CCATTCAGGA CATCAGGAGG 
TTCACTTACG TGGTCAAACT ACTGCAGCTA ATTGCGAAGT CCCAGTTAAC CTCACTGAGT 
GGAGTGGCAC AGAAGAACTA CTTCAACATT TTGGATAAAA TCGTCCAAAA GGTTCTTGAT 
GACCATCAGA ACCCTCGCCT GATCAAAGGC CTCCTCCAGG ACCTGAGCTC CACCCTTGGC 
ATCCTGGTGA GAGGGGTGGG GAAGTCAGTG CTAGTCGGGA ACATCAACAT CTGGATCTGC 
CGCCTGGAGA CCGTCCTCAG CTGGCAGCAG CAGCTGCAGA ACCTCCAGGT GACAAAGGTA 
AACACTGGCC TCACACTCAG TGACCTGCCC CTGCACATGC TGAACAACAT CCTGTACCGT 
TTCTCAGATG GCTGGGACAT TGTCACCCTG GGCCAGGTAA CACCAACCCT GTACATGCTC 
AGTGAAGACA GGCGGCTGTG GAAGCGACTG TGTCAGTACC ACTTTGCTGA GCAGCAGTTT 
TGTAGACATT TGATCCTTTC AGAAAAAGGT CATATCGAGT GGAAGCTGAT GTATTTCACG 
CTTCAGAAGT ATTACCCGAC CAAAGAGCAG TACGGGGACA CGCTACACTT CTGCCGCCAC 
TGCAGCATCC TCTTCTGGAA GGACTACCAC CTTGCTTGGT TGTTGAAGGA CTCGGGACAC 
CCCTGCACCG CAGCTGACCC CGACAGCTGC TTCACTCCTG TGTCTCCGGA GCACTTCATT 
GACCTCTTCA AGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fbxo25 ( XM_006508854.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006508917.1 Mus musculus F-box protein 25 (Fbxo25), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu40443
Clone ID Related Accession (Same CDS sequence)	NM_001359061.1 , XM_006508854.3
Accession Version	XM_006508854.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1101bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	F-box only protein 25 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006508854.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGTTTC TGGGTCAGGA CTGGAGGTCC CCTGGATGGA GCTGGATTAA AACCGAGGAT 
GGGTGGAAAA GGTGTGACCC CTGTAGCCAC GAGCTCAGGA GCGAGGATAG CCAGTACACC 
ATCAACCACA GCATTATCTT AAATAGTGGT GAAGAAGAAA TATTCAACAA TGAGTGTGAA 
TACGCAGCCA AAAAGAGGAA GAAGGAACAT TTTGGAAATG ACACAGCTGC TCACAGTTTT 
TATCGTGAAA AATGGATCTA CGTCCATAAG GAAAGCACAA AGGAAAGGCA TGGCTACTGC 
ACCCTGGGAG AAGCCTTCAA CCGGCTAGAC TTCTCAAGTG CCATTCAGGA CATCAGGAGG 
TTCACTTACG TGGTCAAACT ACTGCAGCTA ATTGCGAAGT CCCAGTTAAC CTCACTGAGT 
GGAGTGGCAC AGAAGAACTA CTTCAACATT TTGGATAAAA TCGTCCAAAA GGTTCTTGAT 
GACCATCAGA ACCCTCGCCT GATCAAAGGC CTCCTCCAGG ACCTGAGCTC CACCCTTGGC 
ATCCTGGTGA GAGGGGTGGG GAAGTCAGTG CTAGTCGGGA ACATCAACAT CTGGATCTGC 
CGCCTGGAGA CCGTCCTCAG CTGGCAGCAG CAGCTGCAGA ACCTCCAGGT GACAAAGCAG 
GTAAACACTG GCCTCACACT CAGTGACCTG CCCCTGCACA TGCTGAACAA CATCCTGTAC 
CGTTTCTCAG ATGGCTGGGA CATTGTCACC CTGGGCCAGG TAACACCAAC CCTGTACATG 
CTCAGTGAAG ACAGGCGGCT GTGGAAGCGA CTGTGTCAGT ACCACTTTGC TGAGCAGCAG 
TTTTGTAGAC ATTTGATCCT TTCAGAAAAA GGTCATATCG AGTGGAAGCT GATGTATTTC 
ACGCTTCAGA AGTATTACCC GACCAAAGAG CAGTACGGGG ACACGCTACA CTTCTGCCGC 
CACTGCAGCA TCCTCTTCTG GAAGGACTAC CACCTTGCTT GGTTGTTGAA GGACTCGGGA 
CACCCCTGCA CCGCAGCTGA CCCCGACAGC TGCTTCACTC CTGTGTCTCC GGAGCACTTC 
ATTGACCTCT TCAAGTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508917.1
CDS	129..1229
Translation	MPFLGQDWRSPGWSWIKTEDGWKRCDPCSHELRSEDSQYTINHSIILNSGEEEIFNNECEYAAKKRKKEHFGNDTAAHSFYREKWIYVHKESTKERHGYCTLGEAFNRLDFSSAIQDIRRFTYVVKLLQLIAKSQLTSLSGVAQKNYFNILDKIVQKVLDDHQNPRLIKGLLQDLSSTLGILVRGVGKSVLVGNINIWICRLETVLSWQQQLQNLQVTKQVNTGLTLSDLPLHMLNNILYRFSDGWDIVTLGQVTPTLYMLSEDRRLWKRLCQYHFAEQQFCRHLILSEKGHIEWKLMYFTLQKYYPTKEQYGDTLHFCRHCSILFWKDYHLAWLLKDSGHPCTAADPDSCFTPVSPEHFIDLFKF

Target ORF information:

RefSeq Version	XM_006508854.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus F-box protein 25 (Fbxo25), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508854.3

ORF Insert Sequence:

ATGCCGTTTC TGGGTCAGGA CTGGAGGTCC CCTGGATGGA GCTGGATTAA AACCGAGGAT 
GGGTGGAAAA GGTGTGACCC CTGTAGCCAC GAGCTCAGGA GCGAGGATAG CCAGTACACC 
ATCAACCACA GCATTATCTT AAATAGTGGT GAAGAAGAAA TATTCAACAA TGAGTGTGAA 
TACGCAGCCA AAAAGAGGAA GAAGGAACAT TTTGGAAATG ACACAGCTGC TCACAGTTTT 
TATCGTGAAA AATGGATCTA CGTCCATAAG GAAAGCACAA AGGAAAGGCA TGGCTACTGC 
ACCCTGGGAG AAGCCTTCAA CCGGCTAGAC TTCTCAAGTG CCATTCAGGA CATCAGGAGG 
TTCACTTACG TGGTCAAACT ACTGCAGCTA ATTGCGAAGT CCCAGTTAAC CTCACTGAGT 
GGAGTGGCAC AGAAGAACTA CTTCAACATT TTGGATAAAA TCGTCCAAAA GGTTCTTGAT 
GACCATCAGA ACCCTCGCCT GATCAAAGGC CTCCTCCAGG ACCTGAGCTC CACCCTTGGC 
ATCCTGGTGA GAGGGGTGGG GAAGTCAGTG CTAGTCGGGA ACATCAACAT CTGGATCTGC 
CGCCTGGAGA CCGTCCTCAG CTGGCAGCAG CAGCTGCAGA ACCTCCAGGT GACAAAGCAG 
GTAAACACTG GCCTCACACT CAGTGACCTG CCCCTGCACA TGCTGAACAA CATCCTGTAC 
CGTTTCTCAG ATGGCTGGGA CATTGTCACC CTGGGCCAGG TAACACCAAC CCTGTACATG 
CTCAGTGAAG ACAGGCGGCT GTGGAAGCGA CTGTGTCAGT ACCACTTTGC TGAGCAGCAG 
TTTTGTAGAC ATTTGATCCT TTCAGAAAAA GGTCATATCG AGTGGAAGCT GATGTATTTC 
ACGCTTCAGA AGTATTACCC GACCAAAGAG CAGTACGGGG ACACGCTACA CTTCTGCCGC 
CACTGCAGCA TCCTCTTCTG GAAGGACTAC CACCTTGCTT GGTTGTTGAA GGACTCGGGA 
CACCCCTGCA CCGCAGCTGA CCCCGACAGC TGCTTCACTC CTGTGTCTCC GGAGCACTTC 
ATTGACCTCT TCAAGTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fbxo25 ( NM_025785.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_080061.1 Mus musculus F-box protein 25 (Fbxo25), transcript variant 2, mRNA.

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CloneID	OMu01027
Clone ID Related Accession (Same CDS sequence)	NM_025785.2 , NM_025785.3
Accession Version	NM_025785.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1074bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-18
Organism	Mus musculus(house mouse)
Product	F-box only protein 25 isforom 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AK018626.1. On Apr 6, 2007 this sequence version replaced NM_025785.1. Transcript Variant: This variant (2) lacks an in-frame exon in the 3' coding region, compared to variant 3. The encoded isoform (2) is shorter than isoform 3. ##Evidence-Data-START## Transcript exon combination :: AK018626.1, AK008027.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGCCGTTTC TGGGTCAGGA CTGGAGGTCC CCTGGATGGA GCTGGATTAA AACCGAGGAT 
GGGTGGAAAA GGTGTGACCC CTGTAGCCAC GAGCTCAGGA GCGAGGATAG CCAGTACACC 
ATCAACCACA GCATTATCTT AAATAGTGGT GAAGAAGAAA TATTCAACAA TGAGTGTGAA 
TACGCAGCCA AAAAGAGGAA GAAGGAACAT TTTGGAAATG ACACAGCTGC TCACAGTTTT 
TATCGTGAAA AATGGATCTA CGTCCATAAG GAAAGCACAA AGGAAAGGCA TGGCTACTGC 
ACCCTGGGAG AAGCCTTCAA CCGGCTAGAC TTCTCAAGTG CCATTCAGGA CATCAGGAGG 
TTCACTTACG TGGTCAAACT ACTGCAGCTA ATTGCGAAGT CCCAGTTAAC CTCACTGAGT 
GGAGTGGCAC AGAAGAACTA CTTCAACATT TTGGATAAAA TCGTCCAAAA GGTTCTTGAT 
GACCATCAGA ACCCTCGCCT GATCAAAGGC CTCCTCCAGG ACCTGAGCTC CACCCTTGGC 
ATCCTGGTGA GAGGGGTGGG GAAGTCAGTG CTAGTCGGGA ACATCAACAT CTGGATCTGC 
CGCCTGGAGA CCGTCCTCAG CTGGCAGCAG CAGCTGCAGA ACCTCCAGGT GACAAAGCAG 
GTAAACACTG GCCTCACACT CAGTGACCTG CCCCTGCACA TGCTGAACAA CATCCTGTAC 
CGTTTCTCAG ATGGCTGGGA CATTGTCACC CTGGGCCAGG TAACACCAAC CCTGTACATG 
CTCAGTGAAG ACAGGCGGCT GTGGAAGCGA CTGTGTCAGT ACCACTTTGC TGAGCAGCAG 
TTTTGTAGAC ATTTGATCCT TTCAGAAAAA GGTCATATCG AGTGGAAGCT GATGTATTTC 
ACGCTTCAGA AGTATTACCC GACCAAAGAG CAGTACGGGG ACACGCTACA CTTCTGCCGC 
CACTGCAGCA TCCTCTTCTG GAAGGACTCG GGACACCCCT GCACCGCAGC TGACCCCGAC 
AGCTGCTTCA CTCCTGTGTC TCCGGAGCAC TTCATTGACC TCTTCAAGTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_080061.1
CDS	66..1139
Translation	MPFLGQDWRSPGWSWIKTEDGWKRCDPCSHELRSEDSQYTINHSIILNSGEEEIFNNECEYAAKKRKKEHFGNDTAAHSFYREKWIYVHKESTKERHGYCTLGEAFNRLDFSSAIQDIRRFTYVVKLLQLIAKSQLTSLSGVAQKNYFNILDKIVQKVLDDHQNPRLIKGLLQDLSSTLGILVRGVGKSVLVGNINIWICRLETVLSWQQQLQNLQVTKQVNTGLTLSDLPLHMLNNILYRFSDGWDIVTLGQVTPTLYMLSEDRRLWKRLCQYHFAEQQFCRHLILSEKGHIEWKLMYFTLQKYYPTKEQYGDTLHFCRHCSILFWKDSGHPCTAADPDSCFTPVSPEHFIDLFKF

Target ORF information:

RefSeq Version	NM_025785.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus F-box protein 25 (Fbxo25), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025785.2

ORF Insert Sequence:

ATGCCGTTTC TGGGTCAGGA CTGGAGGTCC CCTGGATGGA GCTGGATTAA AACCGAGGAT 
GGGTGGAAAA GGTGTGACCC CTGTAGCCAC GAGCTCAGGA GCGAGGATAG CCAGTACACC 
ATCAACCACA GCATTATCTT AAATAGTGGT GAAGAAGAAA TATTCAACAA TGAGTGTGAA 
TACGCAGCCA AAAAGAGGAA GAAGGAACAT TTTGGAAATG ACACAGCTGC TCACAGTTTT 
TATCGTGAAA AATGGATCTA CGTCCATAAG GAAAGCACAA AGGAAAGGCA TGGCTACTGC 
ACCCTGGGAG AAGCCTTCAA CCGGCTAGAC TTCTCAAGTG CCATTCAGGA CATCAGGAGG 
TTCACTTACG TGGTCAAACT ACTGCAGCTA ATTGCGAAGT CCCAGTTAAC CTCACTGAGT 
GGAGTGGCAC AGAAGAACTA CTTCAACATT TTGGATAAAA TCGTCCAAAA GGTTCTTGAT 
GACCATCAGA ACCCTCGCCT GATCAAAGGC CTCCTCCAGG ACCTGAGCTC CACCCTTGGC 
ATCCTGGTGA GAGGGGTGGG GAAGTCAGTG CTAGTCGGGA ACATCAACAT CTGGATCTGC 
CGCCTGGAGA CCGTCCTCAG CTGGCAGCAG CAGCTGCAGA ACCTCCAGGT GACAAAGCAG 
GTAAACACTG GCCTCACACT CAGTGACCTG CCCCTGCACA TGCTGAACAA CATCCTGTAC 
CGTTTCTCAG ATGGCTGGGA CATTGTCACC CTGGGCCAGG TAACACCAAC CCTGTACATG 
CTCAGTGAAG ACAGGCGGCT GTGGAAGCGA CTGTGTCAGT ACCACTTTGC TGAGCAGCAG 
TTTTGTAGAC ATTTGATCCT TTCAGAAAAA GGTCATATCG AGTGGAAGCT GATGTATTTC 
ACGCTTCAGA AGTATTACCC GACCAAAGAG CAGTACGGGG ACACGCTACA CTTCTGCCGC 
CACTGCAGCA TCCTCTTCTG GAAGGACTCG GGACACCCCT GCACCGCAGC TGACCCCGAC 
AGCTGCTTCA CTCCTGTGTC TCCGGAGCAC TTCATTGACC TCTTCAAGTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fbxo25 ( NM_025785.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_080061.1 Mus musculus F-box protein 25 (Fbxo25), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu01027
Clone ID Related Accession (Same CDS sequence)	NM_025785.2 , NM_025785.3
Accession Version	NM_025785.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1074bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Mus musculus(house mouse)
Product	F-box only protein 25 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC139186.4. On Feb 2, 2018 this sequence version replaced NM_025785.2. Transcript Variant: This variant (2) lacks an in-frame exon in the 3' coding region, compared to variant 3. The encoded isoform (2) is shorter than isoform 3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK018626.1, AK008027.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGCCGTTTC TGGGTCAGGA CTGGAGGTCC CCTGGATGGA GCTGGATTAA AACCGAGGAT 
GGGTGGAAAA GGTGTGACCC CTGTAGCCAC GAGCTCAGGA GCGAGGATAG CCAGTACACC 
ATCAACCACA GCATTATCTT AAATAGTGGT GAAGAAGAAA TATTCAACAA TGAGTGTGAA 
TACGCAGCCA AAAAGAGGAA GAAGGAACAT TTTGGAAATG ACACAGCTGC TCACAGTTTT 
TATCGTGAAA AATGGATCTA CGTCCATAAG GAAAGCACAA AGGAAAGGCA TGGCTACTGC 
ACCCTGGGAG AAGCCTTCAA CCGGCTAGAC TTCTCAAGTG CCATTCAGGA CATCAGGAGG 
TTCACTTACG TGGTCAAACT ACTGCAGCTA ATTGCGAAGT CCCAGTTAAC CTCACTGAGT 
GGAGTGGCAC AGAAGAACTA CTTCAACATT TTGGATAAAA TCGTCCAAAA GGTTCTTGAT 
GACCATCAGA ACCCTCGCCT GATCAAAGGC CTCCTCCAGG ACCTGAGCTC CACCCTTGGC 
ATCCTGGTGA GAGGGGTGGG GAAGTCAGTG CTAGTCGGGA ACATCAACAT CTGGATCTGC 
CGCCTGGAGA CCGTCCTCAG CTGGCAGCAG CAGCTGCAGA ACCTCCAGGT GACAAAGCAG 
GTAAACACTG GCCTCACACT CAGTGACCTG CCCCTGCACA TGCTGAACAA CATCCTGTAC 
CGTTTCTCAG ATGGCTGGGA CATTGTCACC CTGGGCCAGG TAACACCAAC CCTGTACATG 
CTCAGTGAAG ACAGGCGGCT GTGGAAGCGA CTGTGTCAGT ACCACTTTGC TGAGCAGCAG 
TTTTGTAGAC ATTTGATCCT TTCAGAAAAA GGTCATATCG AGTGGAAGCT GATGTATTTC 
ACGCTTCAGA AGTATTACCC GACCAAAGAG CAGTACGGGG ACACGCTACA CTTCTGCCGC 
CACTGCAGCA TCCTCTTCTG GAAGGACTCG GGACACCCCT GCACCGCAGC TGACCCCGAC 
AGCTGCTTCA CTCCTGTGTC TCCGGAGCAC TTCATTGACC TCTTCAAGTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_080061.1
CDS	133..1206
Translation	MPFLGQDWRSPGWSWIKTEDGWKRCDPCSHELRSEDSQYTINHSIILNSGEEEIFNNECEYAAKKRKKEHFGNDTAAHSFYREKWIYVHKESTKERHGYCTLGEAFNRLDFSSAIQDIRRFTYVVKLLQLIAKSQLTSLSGVAQKNYFNILDKIVQKVLDDHQNPRLIKGLLQDLSSTLGILVRGVGKSVLVGNINIWICRLETVLSWQQQLQNLQVTKQVNTGLTLSDLPLHMLNNILYRFSDGWDIVTLGQVTPTLYMLSEDRRLWKRLCQYHFAEQQFCRHLILSEKGHIEWKLMYFTLQKYYPTKEQYGDTLHFCRHCSILFWKDSGHPCTAADPDSCFTPVSPEHFIDLFKF

Target ORF information:

RefSeq Version	NM_025785.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus F-box protein 25 (Fbxo25), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025785.3

ORF Insert Sequence:

ATGCCGTTTC TGGGTCAGGA CTGGAGGTCC CCTGGATGGA GCTGGATTAA AACCGAGGAT 
GGGTGGAAAA GGTGTGACCC CTGTAGCCAC GAGCTCAGGA GCGAGGATAG CCAGTACACC 
ATCAACCACA GCATTATCTT AAATAGTGGT GAAGAAGAAA TATTCAACAA TGAGTGTGAA 
TACGCAGCCA AAAAGAGGAA GAAGGAACAT TTTGGAAATG ACACAGCTGC TCACAGTTTT 
TATCGTGAAA AATGGATCTA CGTCCATAAG GAAAGCACAA AGGAAAGGCA TGGCTACTGC 
ACCCTGGGAG AAGCCTTCAA CCGGCTAGAC TTCTCAAGTG CCATTCAGGA CATCAGGAGG 
TTCACTTACG TGGTCAAACT ACTGCAGCTA ATTGCGAAGT CCCAGTTAAC CTCACTGAGT 
GGAGTGGCAC AGAAGAACTA CTTCAACATT TTGGATAAAA TCGTCCAAAA GGTTCTTGAT 
GACCATCAGA ACCCTCGCCT GATCAAAGGC CTCCTCCAGG ACCTGAGCTC CACCCTTGGC 
ATCCTGGTGA GAGGGGTGGG GAAGTCAGTG CTAGTCGGGA ACATCAACAT CTGGATCTGC 
CGCCTGGAGA CCGTCCTCAG CTGGCAGCAG CAGCTGCAGA ACCTCCAGGT GACAAAGCAG 
GTAAACACTG GCCTCACACT CAGTGACCTG CCCCTGCACA TGCTGAACAA CATCCTGTAC 
CGTTTCTCAG ATGGCTGGGA CATTGTCACC CTGGGCCAGG TAACACCAAC CCTGTACATG 
CTCAGTGAAG ACAGGCGGCT GTGGAAGCGA CTGTGTCAGT ACCACTTTGC TGAGCAGCAG 
TTTTGTAGAC ATTTGATCCT TTCAGAAAAA GGTCATATCG AGTGGAAGCT GATGTATTTC 
ACGCTTCAGA AGTATTACCC GACCAAAGAG CAGTACGGGG ACACGCTACA CTTCTGCCGC 
CACTGCAGCA TCCTCTTCTG GAAGGACTCG GGACACCCCT GCACCGCAGC TGACCCCGAC 
AGCTGCTTCA CTCCTGTGTC TCCGGAGCAC TTCATTGACC TCTTCAAGTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fbxo25 ( XM_030243729.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030099589.1 Mus musculus F-box protein 25 (Fbxo25), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu89928
Clone ID Related Accession (Same CDS sequence)	XM_030243729.1
Accession Version	XM_030243729.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1071bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	F-box only protein 25 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGTTTC TGGGTCAGGA CTGGAGGTCC CCTGGATGGA GCTGGATTAA AACCGAGGAT 
GGGTGGAAAA GGTGTGACCC CTGTAGCCAC GAGCTCAGGA GCGAGGATAG CCAGTACACC 
ATCAACCACA GCATTATCTT AAATAGTGGT GAAGAAGAAA TATTCAACAA TGAGTGTGAA 
TACGCAGCCA AAAAGAGGAA GAAGGAACAT TTTGGAAATG ACACAGCTGC TCACAGTTTT 
TATCGTGAAA AATGGATCTA CGTCCATAAG GAAAGCACAA AGGAAAGGCA TGGCTACTGC 
ACCCTGGGAG AAGCCTTCAA CCGGCTAGAC TTCTCAAGTG CCATTCAGGA CATCAGGAGG 
TTCACTTACG TGGTCAAACT ACTGCAGCTA ATTGCGAAGT CCCAGTTAAC CTCACTGAGT 
GGAGTGGCAC AGAAGAACTA CTTCAACATT TTGGATAAAA TCGTCCAAAA GGTTCTTGAT 
GACCATCAGA ACCCTCGCCT GATCAAAGGC CTCCTCCAGG ACCTGAGCTC CACCCTTGGC 
ATCCTGGTGA GAGGGGTGGG GAAGTCAGTG CTAGTCGGGA ACATCAACAT CTGGATCTGC 
CGCCTGGAGA CCGTCCTCAG CTGGCAGCAG CAGCTGCAGA ACCTCCAGGT GACAAAGGTA 
AACACTGGCC TCACACTCAG TGACCTGCCC CTGCACATGC TGAACAACAT CCTGTACCGT 
TTCTCAGATG GCTGGGACAT TGTCACCCTG GGCCAGGTAA CACCAACCCT GTACATGCTC 
AGTGAAGACA GGCGGCTGTG GAAGCGACTG TGTCAGTACC ACTTTGCTGA GCAGCAGTTT 
TGTAGACATT TGATCCTTTC AGAAAAAGGT CATATCGAGT GGAAGCTGAT GTATTTCACG 
CTTCAGAAGT ATTACCCGAC CAAAGAGCAG TACGGGGACA CGCTACACTT CTGCCGCCAC 
TGCAGCATCC TCTTCTGGAA GGACTCGGGA CACCCCTGCA CCGCAGCTGA CCCCGACAGC 
TGCTTCACTC CTGTGTCTCC GGAGCACTTC ATTGACCTCT TCAAGTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030099589.1
CDS	136..1206
Translation	MPFLGQDWRSPGWSWIKTEDGWKRCDPCSHELRSEDSQYTINHSIILNSGEEEIFNNECEYAAKKRKKEHFGNDTAAHSFYREKWIYVHKESTKERHGYCTLGEAFNRLDFSSAIQDIRRFTYVVKLLQLIAKSQLTSLSGVAQKNYFNILDKIVQKVLDDHQNPRLIKGLLQDLSSTLGILVRGVGKSVLVGNINIWICRLETVLSWQQQLQNLQVTKVNTGLTLSDLPLHMLNNILYRFSDGWDIVTLGQVTPTLYMLSEDRRLWKRLCQYHFAEQQFCRHLILSEKGHIEWKLMYFTLQKYYPTKEQYGDTLHFCRHCSILFWKDSGHPCTAADPDSCFTPVSPEHFIDLFKF

Target ORF information:

RefSeq Version	XM_030243729.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus F-box protein 25 (Fbxo25), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030243729.1

ORF Insert Sequence:

ATGCCGTTTC TGGGTCAGGA CTGGAGGTCC CCTGGATGGA GCTGGATTAA AACCGAGGAT 
GGGTGGAAAA GGTGTGACCC CTGTAGCCAC GAGCTCAGGA GCGAGGATAG CCAGTACACC 
ATCAACCACA GCATTATCTT AAATAGTGGT GAAGAAGAAA TATTCAACAA TGAGTGTGAA 
TACGCAGCCA AAAAGAGGAA GAAGGAACAT TTTGGAAATG ACACAGCTGC TCACAGTTTT 
TATCGTGAAA AATGGATCTA CGTCCATAAG GAAAGCACAA AGGAAAGGCA TGGCTACTGC 
ACCCTGGGAG AAGCCTTCAA CCGGCTAGAC TTCTCAAGTG CCATTCAGGA CATCAGGAGG 
TTCACTTACG TGGTCAAACT ACTGCAGCTA ATTGCGAAGT CCCAGTTAAC CTCACTGAGT 
GGAGTGGCAC AGAAGAACTA CTTCAACATT TTGGATAAAA TCGTCCAAAA GGTTCTTGAT 
GACCATCAGA ACCCTCGCCT GATCAAAGGC CTCCTCCAGG ACCTGAGCTC CACCCTTGGC 
ATCCTGGTGA GAGGGGTGGG GAAGTCAGTG CTAGTCGGGA ACATCAACAT CTGGATCTGC 
CGCCTGGAGA CCGTCCTCAG CTGGCAGCAG CAGCTGCAGA ACCTCCAGGT GACAAAGGTA 
AACACTGGCC TCACACTCAG TGACCTGCCC CTGCACATGC TGAACAACAT CCTGTACCGT 
TTCTCAGATG GCTGGGACAT TGTCACCCTG GGCCAGGTAA CACCAACCCT GTACATGCTC 
AGTGAAGACA GGCGGCTGTG GAAGCGACTG TGTCAGTACC ACTTTGCTGA GCAGCAGTTT 
TGTAGACATT TGATCCTTTC AGAAAAAGGT CATATCGAGT GGAAGCTGAT GTATTTCACG 
CTTCAGAAGT ATTACCCGAC CAAAGAGCAG TACGGGGACA CGCTACACTT CTGCCGCCAC 
TGCAGCATCC TCTTCTGGAA GGACTCGGGA CACCCCTGCA CCGCAGCTGA CCCCGACAGC 
TGCTTCACTC CTGTGTCTCC GGAGCACTTC ATTGACCTCT TCAAGTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Fbxo25 controls Tbx5 and Nkx2-5 transcriptional activity to regulate cardiomyocyte development.
Biochimica et biophysica acta1849(6)709-21(2015 Jun)
Jeong HS,Jung ES,Sim YJ,Kim SJ,Jang JW,Hong KS,Lee WY,Chung HM,Park KT,Jung YS,Kim CH,Kim KS

A cluster of cooperating tumor-suppressor gene candidates in chromosomal deletions.
Proceedings of the National Academy of Sciences of the United States of America109(21)8212-7(2012 May)
Xue W,Kitzing T,Roessler S,Zuber J,Krasnitz A,Schultz N,Revill K,Weissmueller S,Rappaport AR,Simon J,Zhang J,Luo W,Hicks J,Zender L,Wang XW,Powers S,Wigler M,Lowe SW

A novel Fbxo25 acts as an E3 ligase for destructing cardiac specific transcription factors.
Biochemical and biophysical research communications410(2)183-8(2011 Jul)
Jang JW,Lee WY,Lee JH,Moon SH,Kim CH,Chung HM

FBXO25-associated nuclear domains: a novel subnuclear structure.
Molecular biology of the cell19(5)1848-61(2008 May)
Manfiolli AO,Maragno AL,Baqui MM,Yokoo S,Teixeira FR,Oliveira EB,Gomes MD

FBXO25, an F-box protein homologue of atrogin-1, is not induced in atrophying muscle.
Biochimica et biophysica acta1760(6)966-72(2006 Jun)
Maragno AL,Baqui MM,Gomes MD

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Fbxo25 cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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