Sbds cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sbds
Entrez Gene ID	66711
Full Name	SBDS ribosome maturation factor
Synonyms	4733401P19Rik,AI836084,CGI-97,SDS
General protein information	Preferred Names SBDS ribosome maturation factor Names ribosome maturation protein SBDS SBDS ribosome assembly guanine nucleotide exchange factor Shwachman-Bodian-Diamond syndrome homolog protein 22A3 shwachman-Bodian-Diamond syndrome protein homolog
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 G1.3
Summary	This gene encodes a protein that is necessary for ribosome function and maintaining normal levels of protein synthesis. The encoded protein may function to activate ribosomes for translation, and may be involved in cellular response to stress and DNA damage. Loss of this gene is embryonic lethal while deficiency of the encoded protein in the pancreas is associated with symptoms of Shwachman-Diamond syndrome. [provided by RefSeq, Dec 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_023248.2	NP_075737.1	ribosome maturation protein SBDS
XM_006504490.2	XP_006504553.1	ribosome maturation protein SBDS isoform X1
NM_023248.1	NP_075737.1	ribosome maturation protein SBDS
XM_006504490.3	XP_006504553.1	ribosome maturation protein SBDS isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SBDS	XP_848632.1
Drosophila melanogaster (fruit fly)	CG8549	NP_001261482.1
Kluyveromyces lactis	KLLA0F15532g	XP_455778.1
Neurospora crassa	NCU00476	XP_956795.1
Pan troglodytes (chimpanzee)	SBDS	XP_519568.2
Gallus gallus (chicken)	SBDS	NP_001006211.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007882	XP_317609.3
Caenorhabditis elegans (roundworm)	sbds-1	NP_497226.2
Gallus gallus (chicken)	LOC100858362	XP_003642436.1
Eremothecium gossypii	AGOS_AAL154C	NP_982388.1
Schizosaccharomyces pombe (fission yeast)	sdo1	NP_593869.1
Magnaporthe oryzae (rice blast fungus)	MGG_05662	XP_003710560.1
Homo sapiens (human)	SBDS	NP_057122.2
Rattus norvegicus (Norway rat)	Sbds	NP_001008290.1
Danio rerio (zebrafish)	sbds	NP_957415.1
Arabidopsis thaliana (thale cress)	AT1G43860	NP_564488.1
Oryza sativa (rice)	Os08g0107500	NP_001060798.1
Bos taurus (cattle)	SBDS	NP_001029611.1
Mus musculus (house mouse)	Sbds	NP_075737.1
Saccharomyces cerevisiae (baker's yeast)	SDO1	NP_013122.1
Xenopus tropicalis (tropical clawed frog)	sbds	NP_001004953.1

Gene Ontology
Bibliography

Related articles in PubMed

Deficiency of the ribosome biogenesis gene Sbds in hematopoietic stem and progenitor cells causes neutropenia in mice by attenuating lineage progression in myelocytes.
Zambetti NA, Bindels EM, Van Strien PM, Valkhof MG, Adisty MN, Hoogenboezem RM, Sanders MA, Rommens JM, Touw IP, Raaijmakers MH
Haematologica100(10)1285-93(2015 Oct)

In Vivo Senescence in the Sbds-Deficient Murine Pancreas: Cell-Type Specific Consequences of Translation Insufficiency.
Tourlakis ME, Zhang S, Ball HL, Gandhi R, Liu H, Zhong J, Yuan JS, Guidos CJ, Durie PR, Rommens JM
PLoS genetics11(6)e1005288(2015 Jun)

Deficiency of Sbds in the mouse pancreas leads to features of Shwachman-Diamond syndrome, with loss of zymogen granules.
Tourlakis ME, Zhong J, Gandhi R, Zhang S, Chen L, Durie PR, Rommens JM
Gastroenterology143(2)481-92(2012 Aug)

Uncoupling of GTP hydrolysis from eIF6 release on the ribosome causes Shwachman-Diamond syndrome.
Finch AJ, Hilcenko C, Basse N, Drynan LF, Goyenechea B, Menne TF, Gonz?lez Fern?ndez A, Simpson P, D'Santos CS, Arends MJ, Donadieu J, Bellann?-Chantelot C, Costanzo M, Boone C, McKenzie AN, Freund SM, Warren AJ
Genes & development25(9)917-29(2011 May)

Sbds is required for Rac2-mediated monocyte migration and signaling downstream of RANK during osteoclastogenesis.
Leung R, Cuddy K, Wang Y, Rommens J, Glogauer M
Blood117(6)2044-53(2011 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Deficiency of the ribosome biogenesis gene Sbds in hematopoietic stem and progenitor cells causes neutropenia in mice by attenuating lineage progression in myelocytes
Title: Deficiency of the ribosome biogenesis gene Sbds in hematopoietic stem and progenitor cells causes neutropenia in mice by attenuating lineage progression in myelocytes.

Sbds genotypes correlated with phenotypes in a mouse model of Shwachman-Diamond syndrome. Defects developed specifically in the pancreata of mice, reducing growth of mice and production of digestive enzymes.
Title: Deficiency of Sbds in the mouse pancreas leads to features of Shwachman-Diamond syndrome, with loss of zymogen granules.

Sbds is required for osteoclastogenesis by regulating monocyte migration via Rac2 and osteoclast differentiation signaling downstream of RANK.
Title: Sbds is required for Rac2-mediated monocyte migration and signaling downstream of RANK during osteoclastogenesis.

The majority of Shwachman-Diamond syndrome cases are results of mutations in SBDS gene on chromosone 7q11.
Title: Shwachman-Diamond syndrome presenting as hypoglycemia.

loss of Sbds is sufficient to induce abnormalities in hematopoiesis
Title: Lentiviral-mediated RNAi inhibition of Sbds in murine hematopoietic progenitors impairs their hematopoietic potential.

The following Sbds gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sbds cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu00929	NM_023248.2 Latest version!	Mus musculus SBDS ribosome maturation factor (Sbds), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu36187	XM_006504490.3 Latest version!	Mus musculus SBDS ribosome maturation factor (Sbds), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OMu00929	NM_023248.1	Mus musculus Shwachman-Bodian-Diamond syndrome homolog (human) (Sbds), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu36187	XM_006504490.2	Mus musculus Shwachman-Bodian-Diamond syndrome homolog (human) (Sbds), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sbds ( XM_006504490.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006504553.1 Mus musculus Shwachman-Bodian-Diamond syndrome homolog (human) (Sbds), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu36187
Clone ID Related Accession (Same CDS sequence)	XM_006504490.2 , XM_006504490.3
Accession Version	XM_006504490.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 435bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	ribosome maturation protein SBDS isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006504490.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTAGGG ATATCGCCAC CATTGTGGCA GACAAGTGTG TGAACCCAGA AACAAAGAGA 
CCTTACACCG TTATCCTCAT CGAGAGAGCC ATGAAGGACA TCCACTACTC CGTGAAACCC 
AACAAGAGCA CAAAGCAACA GGCTTTGGAA GTGATAAAGC AGCTGAAAGA GAAGATGAAG 
ATAGAGCGGG CCCACATGCG ATTGCGCTTC ATCCTGCCAG TGAACGAAGG GAAGAAGCTG 
AAGGAGAAGC TGAAGCCACT GATGAAGGTG GTGGAGAGTG AGGACTACAG CCAGCAGCTG 
GAGATCGTGT GCCTCATCGA CCCAGGCTGC TTCAGAGAAA TTGATGAGCT AATAAAAAAG 
GAAACGAAAG GCAGGGGTTC TCTGGAAGTG CTCAGTCTGA AGGACGTGGA GGAAGGCGAT 
GAGAAGTTTG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006504553.1
CDS	233..667
Translation	MFRDIATIVADKCVNPETKRPYTVILIERAMKDIHYSVKPNKSTKQQALEVIKQLKEKMKIERAHMRLRFILPVNEGKKLKEKLKPLMKVVESEDYSQQLEIVCLIDPGCFREIDELIKKETKGRGSLEVLSLKDVEEGDEKFE

Target ORF information:

RefSeq Version	XM_006504490.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus Shwachman-Bodian-Diamond syndrome homolog (human) (Sbds), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006504490.2

ORF Insert Sequence:

ATGTTTAGGG ATATCGCCAC CATTGTGGCA GACAAGTGTG TGAACCCAGA AACAAAGAGA 
CCTTACACCG TTATCCTCAT CGAGAGAGCC ATGAAGGACA TCCACTACTC CGTGAAACCC 
AACAAGAGCA CAAAGCAACA GGCTTTGGAA GTGATAAAGC AGCTGAAAGA GAAGATGAAG 
ATAGAGCGGG CCCACATGCG ATTGCGCTTC ATCCTGCCAG TGAACGAAGG GAAGAAGCTG 
AAGGAGAAGC TGAAGCCACT GATGAAGGTG GTGGAGAGTG AGGACTACAG CCAGCAGCTG 
GAGATCGTGT GCCTCATCGA CCCAGGCTGC TTCAGAGAAA TTGATGAGCT AATAAAAAAG 
GAAACGAAAG GCAGGGGTTC TCTGGAAGTG CTCAGTCTGA AGGACGTGGA GGAAGGCGAT 
GAGAAGTTTG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sbds ( NM_023248.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_075737.1 Mus musculus SBDS ribosome maturation factor (Sbds), mRNA.

Price & Availability↑

CloneID	OMu00929
Clone ID Related Accession (Same CDS sequence)	NM_023248.2 , NM_023248.1
Accession Version	NM_023248.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 753bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Mus musculus(house mouse)
Product	ribosome maturation protein SBDS
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY311303.1, AK014648.1 and AI836084.1. On Dec 2, 2015 this sequence version replaced NM_023248.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC003849.1, SRR1660817.258659.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGATCT TCACCCCCAC CAACCAGATC CGACTGACCA ATGTGGCCGT GGTGCGGATG 
AAGCGGGGAG GGAAGCGCTT CGAAATCGCC TGCTATAAAA ACAAGGTCGT CGGCTGGCGG 
AGTGGCGTGG AAAAAGACCT TGATGAAGTT CTGCAGACCC ATTCAGTGTT TGTAAATGTT 
TCCAAAGGTC AGGTTGCCAA GAAGGAAGAC CTCATCAGTG CATTTGGGAC AGACGACCAG 
ACTGAAATCT GCAAGCAGAT TTTGACTAAA GGAGAAGTTC AAGTGTCAGA TAAAGAACGG 
CACACACAGC TGGAGCAGAT GTTTAGGGAT ATCGCCACCA TTGTGGCAGA CAAGTGTGTG 
AACCCAGAAA CAAAGAGACC TTACACCGTT ATCCTCATCG AGAGAGCCAT GAAGGACATC 
CACTACTCCG TGAAACCCAA CAAGAGCACA AAGCAACAGG CTTTGGAAGT GATAAAGCAG 
CTGAAAGAGA AGATGAAGAT AGAGCGGGCC CACATGCGAT TGCGCTTCAT CCTGCCAGTG 
AACGAAGGGA AGAAGCTGAA GGAGAAGCTG AAGCCACTGA TGAAGGTGGT GGAGAGTGAG 
GACTACAGCC AGCAGCTGGA GATCGTGTGC CTCATCGACC CAGGCTGCTT CAGAGAAATT 
GATGAGCTAA TAAAAAAGGA AACGAAAGGC AGGGGTTCTC TGGAAGTGCT CAGTCTGAAG 
GACGTGGAGG AAGGCGATGA GAAGTTTGAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_075737.1
CDS	194..946
Translation	MSIFTPTNQIRLTNVAVVRMKRGGKRFEIACYKNKVVGWRSGVEKDLDEVLQTHSVFVNVSKGQVAKKEDLISAFGTDDQTEICKQILTKGEVQVSDKERHTQLEQMFRDIATIVADKCVNPETKRPYTVILIERAMKDIHYSVKPNKSTKQQALEVIKQLKEKMKIERAHMRLRFILPVNEGKKLKEKLKPLMKVVESEDYSQQLEIVCLIDPGCFREIDELIKKETKGRGSLEVLSLKDVEEGDEKFE

Target ORF information:

RefSeq Version	NM_023248.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus SBDS ribosome maturation factor (Sbds), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_023248.2

ORF Insert Sequence:

ATGTCGATCT TCACCCCCAC CAACCAGATC CGACTGACCA ATGTGGCCGT GGTGCGGATG 
AAGCGGGGAG GGAAGCGCTT CGAAATCGCC TGCTATAAAA ACAAGGTCGT CGGCTGGCGG 
AGTGGCGTGG AAAAAGACCT TGATGAAGTT CTGCAGACCC ATTCAGTGTT TGTAAATGTT 
TCCAAAGGTC AGGTTGCCAA GAAGGAAGAC CTCATCAGTG CATTTGGGAC AGACGACCAG 
ACTGAAATCT GCAAGCAGAT TTTGACTAAA GGAGAAGTTC AAGTGTCAGA TAAAGAACGG 
CACACACAGC TGGAGCAGAT GTTTAGGGAT ATCGCCACCA TTGTGGCAGA CAAGTGTGTG 
AACCCAGAAA CAAAGAGACC TTACACCGTT ATCCTCATCG AGAGAGCCAT GAAGGACATC 
CACTACTCCG TGAAACCCAA CAAGAGCACA AAGCAACAGG CTTTGGAAGT GATAAAGCAG 
CTGAAAGAGA AGATGAAGAT AGAGCGGGCC CACATGCGAT TGCGCTTCAT CCTGCCAGTG 
AACGAAGGGA AGAAGCTGAA GGAGAAGCTG AAGCCACTGA TGAAGGTGGT GGAGAGTGAG 
GACTACAGCC AGCAGCTGGA GATCGTGTGC CTCATCGACC CAGGCTGCTT CAGAGAAATT 
GATGAGCTAA TAAAAAAGGA AACGAAAGGC AGGGGTTCTC TGGAAGTGCT CAGTCTGAAG 
GACGTGGAGG AAGGCGATGA GAAGTTTGAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sbds ( NM_023248.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_075737.1 Mus musculus Shwachman-Bodian-Diamond syndrome homolog (human) (Sbds), mRNA.

Price & Availability↑

CloneID	OMu00929
Clone ID Related Accession (Same CDS sequence)	NM_023248.2 , NM_023248.1
Accession Version	NM_023248.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 753bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-14
Organism	Mus musculus(House mouse)
Product	ribosome maturation protein SBDS
Comment

ATGTCGATCT TCACCCCCAC CAACCAGATC CGACTGACCA ATGTGGCCGT GGTGCGGATG 
AAGCGGGGAG GGAAGCGCTT CGAAATCGCC TGCTATAAAA ACAAGGTCGT CGGCTGGCGG 
AGTGGCGTGG AAAAAGACCT TGATGAAGTT CTGCAGACCC ATTCAGTGTT TGTAAATGTT 
TCCAAAGGTC AGGTTGCCAA GAAGGAAGAC CTCATCAGTG CATTTGGGAC AGACGACCAG 
ACTGAAATCT GCAAGCAGAT TTTGACTAAA GGAGAAGTTC AAGTGTCAGA TAAAGAACGG 
CACACACAGC TGGAGCAGAT GTTTAGGGAT ATCGCCACCA TTGTGGCAGA CAAGTGTGTG 
AACCCAGAAA CAAAGAGACC TTACACCGTT ATCCTCATCG AGAGAGCCAT GAAGGACATC 
CACTACTCCG TGAAACCCAA CAAGAGCACA AAGCAACAGG CTTTGGAAGT GATAAAGCAG 
CTGAAAGAGA AGATGAAGAT AGAGCGGGCC CACATGCGAT TGCGCTTCAT CCTGCCAGTG 
AACGAAGGGA AGAAGCTGAA GGAGAAGCTG AAGCCACTGA TGAAGGTGGT GGAGAGTGAG 
GACTACAGCC AGCAGCTGGA GATCGTGTGC CTCATCGACC CAGGCTGCTT CAGAGAAATT 
GATGAGCTAA TAAAAAAGGA AACGAAAGGC AGGGGTTCTC TGGAAGTGCT CAGTCTGAAG 
GACGTGGAGG AAGGCGATGA GAAGTTTGAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_075737.1
Translation	MSIFTPTNQIRLTNVAVVRMKRGGKRFEIACYKNKVVGWRSGVEKDLDEVLQTHSVFVNVSKGQVAKKEDLISAFGTDDQTEICKQILTKGEVQVSDKERHTQLEQMFRDIATIVADKCVNPETKRPYTVILIERAMKDIHYSVKPNKSTKQQALEVIKQLKEKMKIERAHMRLRFILPVNEGKKLKEKLKPLMKVVESEDYSQQLEIVCLIDPGCFREIDELIKKETKGRGSLEVLSLKDVEEGDEKFE

Target ORF information:

RefSeq Version	NM_023248.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus Shwachman-Bodian-Diamond syndrome homolog (human) (Sbds), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_023248.1

ORF Insert Sequence:

ATGTCGATCT TCACCCCCAC CAACCAGATC CGACTGACCA ATGTGGCCGT GGTGCGGATG 
AAGCGGGGAG GGAAGCGCTT CGAAATCGCC TGCTATAAAA ACAAGGTCGT CGGCTGGCGG 
AGTGGCGTGG AAAAAGACCT TGATGAAGTT CTGCAGACCC ATTCAGTGTT TGTAAATGTT 
TCCAAAGGTC AGGTTGCCAA GAAGGAAGAC CTCATCAGTG CATTTGGGAC AGACGACCAG 
ACTGAAATCT GCAAGCAGAT TTTGACTAAA GGAGAAGTTC AAGTGTCAGA TAAAGAACGG 
CACACACAGC TGGAGCAGAT GTTTAGGGAT ATCGCCACCA TTGTGGCAGA CAAGTGTGTG 
AACCCAGAAA CAAAGAGACC TTACACCGTT ATCCTCATCG AGAGAGCCAT GAAGGACATC 
CACTACTCCG TGAAACCCAA CAAGAGCACA AAGCAACAGG CTTTGGAAGT GATAAAGCAG 
CTGAAAGAGA AGATGAAGAT AGAGCGGGCC CACATGCGAT TGCGCTTCAT CCTGCCAGTG 
AACGAAGGGA AGAAGCTGAA GGAGAAGCTG AAGCCACTGA TGAAGGTGGT GGAGAGTGAG 
GACTACAGCC AGCAGCTGGA GATCGTGTGC CTCATCGACC CAGGCTGCTT CAGAGAAATT 
GATGAGCTAA TAAAAAAGGA AACGAAAGGC AGGGGTTCTC TGGAAGTGCT CAGTCTGAAG 
GACGTGGAGG AAGGCGATGA GAAGTTTGAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sbds ( XM_006504490.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006504553.1 Mus musculus SBDS ribosome maturation factor (Sbds), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu36187
Clone ID Related Accession (Same CDS sequence)	XM_006504490.2 , XM_006504490.3
Accession Version	XM_006504490.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 435bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ribosome maturation protein SBDS isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006504490.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTAGGG ATATCGCCAC CATTGTGGCA GACAAGTGTG TGAACCCAGA AACAAAGAGA 
CCTTACACCG TTATCCTCAT CGAGAGAGCC ATGAAGGACA TCCACTACTC CGTGAAACCC 
AACAAGAGCA CAAAGCAACA GGCTTTGGAA GTGATAAAGC AGCTGAAAGA GAAGATGAAG 
ATAGAGCGGG CCCACATGCG ATTGCGCTTC ATCCTGCCAG TGAACGAAGG GAAGAAGCTG 
AAGGAGAAGC TGAAGCCACT GATGAAGGTG GTGGAGAGTG AGGACTACAG CCAGCAGCTG 
GAGATCGTGT GCCTCATCGA CCCAGGCTGC TTCAGAGAAA TTGATGAGCT AATAAAAAAG 
GAAACGAAAG GCAGGGGTTC TCTGGAAGTG CTCAGTCTGA AGGACGTGGA GGAAGGCGAT 
GAGAAGTTTG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006504553.1
CDS	420..854
Translation	MFRDIATIVADKCVNPETKRPYTVILIERAMKDIHYSVKPNKSTKQQALEVIKQLKEKMKIERAHMRLRFILPVNEGKKLKEKLKPLMKVVESEDYSQQLEIVCLIDPGCFREIDELIKKETKGRGSLEVLSLKDVEEGDEKFE

Target ORF information:

RefSeq Version	XM_006504490.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus SBDS ribosome maturation factor (Sbds), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006504490.3

ORF Insert Sequence:

ATGTTTAGGG ATATCGCCAC CATTGTGGCA GACAAGTGTG TGAACCCAGA AACAAAGAGA 
CCTTACACCG TTATCCTCAT CGAGAGAGCC ATGAAGGACA TCCACTACTC CGTGAAACCC 
AACAAGAGCA CAAAGCAACA GGCTTTGGAA GTGATAAAGC AGCTGAAAGA GAAGATGAAG 
ATAGAGCGGG CCCACATGCG ATTGCGCTTC ATCCTGCCAG TGAACGAAGG GAAGAAGCTG 
AAGGAGAAGC TGAAGCCACT GATGAAGGTG GTGGAGAGTG AGGACTACAG CCAGCAGCTG 
GAGATCGTGT GCCTCATCGA CCCAGGCTGC TTCAGAGAAA TTGATGAGCT AATAAAAAAG 
GAAACGAAAG GCAGGGGTTC TCTGGAAGTG CTCAGTCTGA AGGACGTGGA GGAAGGCGAT 
GAGAAGTTTG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Deficiency of the ribosome biogenesis gene Sbds in hematopoietic stem and progenitor cells causes neutropenia in mice by attenuating lineage progression in myelocytes.
Haematologica100(10)1285-93(2015 Oct)
Zambetti NA,Bindels EM,Van Strien PM,Valkhof MG,Adisty MN,Hoogenboezem RM,Sanders MA,Rommens JM,Touw IP,Raaijmakers MH

In Vivo Senescence in the Sbds-Deficient Murine Pancreas: Cell-Type Specific Consequences of Translation Insufficiency.
PLoS genetics11(6)e1005288(2015 Jun)
Tourlakis ME,Zhang S,Ball HL,Gandhi R,Liu H,Zhong J,Yuan JS,Guidos CJ,Durie PR,Rommens JM

Deficiency of Sbds in the mouse pancreas leads to features of Shwachman-Diamond syndrome, with loss of zymogen granules.
Gastroenterology143(2)481-92(2012 Aug)
Tourlakis ME,Zhong J,Gandhi R,Zhang S,Chen L,Durie PR,Rommens JM

Uncoupling of GTP hydrolysis from eIF6 release on the ribosome causes Shwachman-Diamond syndrome.
Genes & development25(9)917-29(2011 May)
Finch AJ,Hilcenko C,Basse N,Drynan LF,Goyenechea B,Menne TF,Gonz?lez Fern?ndez A,Simpson P,D'Santos CS,Arends MJ,Donadieu J,Bellann?-Chantelot C,Costanzo M,Boone C,McKenzie AN,Freund SM,Warren AJ

Sbds is required for Rac2-mediated monocyte migration and signaling downstream of RANK during osteoclastogenesis.
Blood117(6)2044-53(2011 Feb)
Leung R,Cuddy K,Wang Y,Rommens J,Glogauer M

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Sbds cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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