GAPVD1 cDNA ORF clone, Mus musculus(House mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	GAPVD1
Entrez Gene ID	66691
Full Name	GTPase activating protein and VPS9 domains 1
Synonyms	2010005B09Rik,4432404J10Rik,AW108497,Gapex-5,RAP6,RME-6,mKIAA1521
General protein information	Preferred Names GTPase activating protein and VPS9 domains 1 Names GTPase-activating protein and VPS9 domain-containing protein 1 rab5-activating protein 6
Gene Type	protein-coding
Organism	Mus musculus(House mouse)
Genome	Chromosome: 2 Map Location: 2\|2 B

mRNA and Protein(s)

mRNA	Protein	Name
XM_011239149.2	XP_011237451.1	GTPase-activating protein and VPS9 domain-containing protein 1 isoform X4
XM_017319198.1	XP_017174687.1	GTPase-activating protein and VPS9 domain-containing protein 1 isoform X7
NM_025709.3	NP_079985.2	GTPase-activating protein and VPS9 domain-containing protein 1 isoform 2
XM_017319195.2	XP_017174684.1	GTPase-activating protein and VPS9 domain-containing protein 1 isoform X1
XM_030251954.1	XP_030107814.1	GTPase-activating protein and VPS9 domain-containing protein 1 isoform X2
XM_011239150.3	XP_011237452.1	GTPase-activating protein and VPS9 domain-containing protein 1 isoform X4
XM_030251956.1	XP_030107816.1	GTPase-activating protein and VPS9 domain-containing protein 1 isoform X5
XM_017319197.2	XP_017174686.1	GTPase-activating protein and VPS9 domain-containing protein 1 isoform X6
XM_011239147.1	XP_011237449.1	GTPase-activating protein and VPS9 domain-containing protein 1 isoform X2
XM_017319196.2	XP_017174685.1	GTPase-activating protein and VPS9 domain-containing protein 1 isoform X1
XM_030251955.1	XP_030107815.1	GTPase-activating protein and VPS9 domain-containing protein 1 isoform X3
XM_011239145.1	XP_011237447.1	GTPase-activating protein and VPS9 domain-containing protein 1 isoform X1
XM_017319195.1	XP_017174684.1	GTPase-activating protein and VPS9 domain-containing protein 1 isoform X1
XM_017319196.1	XP_017174685.1	GTPase-activating protein and VPS9 domain-containing protein 1 isoform X1
XM_011239150.2	XP_011237452.1	GTPase-activating protein and VPS9 domain-containing protein 1 isoform X5
XM_017319197.1	XP_017174686.1	GTPase-activating protein and VPS9 domain-containing protein 1 isoform X6
XM_017319199.1	XP_017174688.1	GTPase-activating protein and VPS9 domain-containing protein 1 isoform X8
XM_030251957.1	XP_030107817.1	GTPase-activating protein and VPS9 domain-containing protein 1 isoform X7
XM_030251958.1	XP_030107818.1	GTPase-activating protein and VPS9 domain-containing protein 1 isoform X8
NM_025709.2	NP_079985.2	GTPase-activating protein and VPS9 domain-containing protein 1
NM_001356441.1	NP_001343370.1	GTPase-activating protein and VPS9 domain-containing protein 1 isoform 1
XM_011239148.2	XP_011237450.1	GTPase-activating protein and VPS9 domain-containing protein 1 isoform X3

Pathways from BioSystems

Homologies

Bos taurus (cattle)	GAPVD1	NP_001095990.1
Rattus norvegicus (Norway rat)	Gapvd1	XP_006234089.1
Homo sapiens (human)	GAPVD1	NP_001269608.1
Pan troglodytes (chimpanzee)	GAPVD1	XP_520259.2
Canis lupus familiaris (dog)	GAPVD1	XP_537846.3
Mus musculus (house mouse)	Gapvd1	NP_079985.2
Gallus gallus (chicken)	GAPVD1	XP_415380.3
Danio rerio (zebrafish)	gapvd1	NP_001002420.2
Xenopus tropicalis (tropical clawed frog)	gapvd1	XP_004916795.1

Gene Ontology
Bibliography

Related articles in PubMed

A high-resolution anatomical atlas of the transcriptome in the mouse embryo.
Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, Magen A, Canidio E, Pagani M, Peluso I, Lin-Marq N, Koch M, Bilio M, Cantiello I, Verde R, De Masi C, Bianchi SA, Cicchini J, Perroud E, Mehmeti S, Dagand E, Schrinner S, N?rnberger A, Schmidt K, Metz K, Zwingmann C, Brieske N, Springer C, Hernandez AM, Herzog S, Grabbe F, Sieverding C, Fischer B, Schrader K, Brockmeyer M, Dettmer S, Helbig C, Alunni V, Battaini MA, Mura C, Henrichsen CN, Garcia-Lopez R, Echevarria D, Puelles E, Garcia-Calero E, Kruse S, Uhr M, Kauck C, Feng G, Milyaev N, Ong CK, Kumar L, Lam M, Semple CA, Gyenesei A, Mundlos S, Radelof U, Lehrach H, Sarmientos P, Reymond A, Davidson DR, Doll? P, Antonarakis SE, Yaspo ML, Martinez S, Baldock RA, Eichele G, Ballabio A
PLoS biology9(1)e1000582(2011 Jan)

Rab22B is expressed in the CNS astroglia lineage and plays a role in epidermal growth factor receptor trafficking in A431 cells.
Ng EL, Ng JJ, Liang F, Tang BL
Journal of cellular physiology221(3)716-28(2009 Dec)

Large-scale gene trapping in C57BL/6N mouse embryonic stem cells.
Hansen GM, Markesich DC, Burnett MB, Zhu Q, Dionne KM, Richter LJ, Finnell RH, Sands AT, Zambrowicz BP, Abuin A
Genome research18(10)1670-9(2008 Oct)

Insulin stimulates phosphatidylinositol 3-phosphate production via the activation of Rab5.
Lodhi IJ, Bridges D, Chiang SH, Zhang Y, Cheng A, Geletka LM, Weisman LS, Saltiel AR
Molecular biology of the cell19(7)2718-28(2008 Jul)

Imaging of Rab5 activity identifies essential regulators for phagosome maturation.
Kitano M, Nakaya M, Nakamura T, Nagata S, Matsuda M
Nature453(7192)241-5(2008 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the TC10/GAPEX-5/Rab5 axis mediates insulin-stimulated production of PI(3)P, which regulates trafficking of Glut4 vesicles
Title: Insulin stimulates phosphatidylinositol 3-phosphate production via the activation of Rab5.

Insulin recruits the CIP4/Gapex-5 complex to the plasma membrane, thus reducing Rab31 activity and permitting Glut4 vesicles to translocate to the cell surface, where Glut4 docks and fuses to transport glucose into the cell.
Title: Gapex-5, a Rab31 guanine nucleotide exchange factor that regulates Glut4 trafficking in adipocytes.

The following GAPVD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GAPVD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu80384	XM_017319199.1 Latest version!	Mus musculus GTPase activating protein and VPS9 domains 1 (Gapvd1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$307.30 $439.00
OMu80382	XM_017319197.2 Latest version!	Mus musculus GTPase activating protein and VPS9 domains 1 (Gapvd1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu80383	XM_017319198.1 Latest version!	Mus musculus GTPase activating protein and VPS9 domains 1 (Gapvd1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu87969	XM_030251957.1 Latest version!	Mus musculus GTPase activating protein and VPS9 domains 1 (Gapvd1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu87970	XM_030251958.1 Latest version!	Mus musculus GTPase activating protein and VPS9 domains 1 (Gapvd1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu00913	NM_025709.3 Latest version!	Mus musculus GTPase activating protein and VPS9 domains 1 (Gapvd1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$846.30 $1209.00
OMu69988	XM_017319196.2 Latest version!	Mus musculus GTPase activating protein and VPS9 domains 1 (Gapvd1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu69988	XM_017319195.2 Latest version!	Mus musculus GTPase activating protein and VPS9 domains 1 (Gapvd1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu69989	XM_030251954.1 Latest version!	Mus musculus GTPase activating protein and VPS9 domains 1 (Gapvd1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu69991	XM_030251955.1 Latest version!	Mus musculus GTPase activating protein and VPS9 domains 1 (Gapvd1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu69992	XM_011239150.3 Latest version!	Mus musculus GTPase activating protein and VPS9 domains 1 (Gapvd1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu87968	XM_030251956.1 Latest version!	Mus musculus GTPase activating protein and VPS9 domains 1 (Gapvd1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu69990	NM_001356441.1 Latest version!	Mus musculus GTPase activating protein and VPS9 domains 1 (Gapvd1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu69988	XM_011239145.1 Latest version!	Mus musculus GTPase activating protein and VPS9 domains 1 (Gapvd1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu69989	XM_011239147.1 Latest version!	Mus musculus GTPase activating protein and VPS9 domains 1 (Gapvd1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu69990	XM_011239148.2 Latest version!	Mus musculus GTPase activating protein and VPS9 domains 1 (Gapvd1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu69991	XM_011239149.2 Latest version!	Mus musculus GTPase activating protein and VPS9 domains 1 (Gapvd1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
View Old Accession Versions >>
OMu69988	XM_017319195.1	Mus musculus GTPase activating protein and VPS9 domains 1 (Gapvd1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu69988	XM_017319196.1	Mus musculus GTPase activating protein and VPS9 domains 1 (Gapvd1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu69992	XM_011239150.2	Mus musculus GTPase activating protein and VPS9 domains 1 (Gapvd1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu80382	XM_017319197.1	Mus musculus GTPase activating protein and VPS9 domains 1 (Gapvd1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$391.30 $559.00
OMu00913	NM_025709.2	Mus musculus GTPase activating protein and VPS9 domains 1 (Gapvd1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$846.30 $1209.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GAPVD1 ( XM_017319197.1 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_017174686.1 Mus musculus GTPase activating protein and VPS9 domains 1 (Gapvd1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OMu80382
Clone ID Related Accession (Same CDS sequence)	XM_017319197.2 , XM_017319197.1
Accession Version	XM_017319197.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2466bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	GTPase-activating protein and VPS9 domain-containing protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGGAC TAACAGATGA CAGGGACATA TCCGAGACTG TGAGTGAGAC CTGGAGCACT 
GATGTCCTGG GAAGTGACTT CGACCCGAAC GTTGATGAAG ATCGTTTGCA AGAGATTGCA 
GGTGCAGCAG CAGAGAACGT GTTAGGTAGT TTGCTGTGCC TTCCAGGTTC AGGGTCAGTT 
CTCCTTGACC CTTGCACTGG CTCTACCATA TCAGAGACCA CAAGTGAAGC TTGGAGTGTA 
GAGGTATTGC CAAGTGACTC AGAGGCTCCA GACTTAAAGC AGGAAGAGCG CCTCCAAGAG 
CTGGAGAGCT GTTCTGGACT GGGCAGCACA TCTGATGATA CGGATGTCAG GGAGGTCAGT 
TCCCGCCCCA GCACACCAGG CCTCAGTGTT GTGTCCGGTA TAAGTGCTAC ATCGGAGGAT 
ATTCCCAATA AGATCGAAGA CCTGAGATCT GAGTGCAGCT CTGATTTTGG GGGCAAAGAT 
TCTGTAACTA GTCCAGATAT GGATGACATA GCCCATGGCG CTCATCAGCT GACCTCTCCT 
CCCTCTCAGT CAGAGTCTCT GCTTGCCATG TTTGATCCAC TGTCCTCACA TGAAGGGGCC 
TCTGCTGTGG TAAGGCCAAA GGTTCACTAT GCCCGACCCT CGCATCCACC TCCGGATCCT 
CCAATCCTGG AAGGTGCAGT GGGAGGAAAT GAGGCCAGGT TGCCAAACTT TGGCTCTCAC 
GTTTTAACTG CAGCTGAAAT GGAGGCGTTC AAGCAGAGAC ACTCATACCC TGAGAGATTG 
GTTCGCAGCA GAAGCTCTGA TATAGTGTCT TCTGTGCGGC GGCCCATGAG TGACCCCAGC 
TGGAACCGAC GTCCAGGGAA TGAAGAACTT CCTCCAGCTG CAGCCACTGG TGCTACTTCT 
TTGGTGGCTG CACCTCACTC GTCATCTTCG TCCCCGAGTA AGGACTCTTC TAGAGGAGAG 
ACTGAGGAAC GCAAGGACAG TGACGATGAG AGGTCAGACA GGAGCAGGCC TTGGTGGAGG 
AAGCGCTTTG TTTCAGCTAT GCCTAAAGCT CCTATACCAT TTAGAAAGAA AGAAAAACAA 
GAAAAAGACA AAGATGATCT GGGTCCTGAC AGATTCTCAA CACTCACAGA TGAGCCCAGC 
CCTAGGCTCA GTGCTCAAGC TCAGGTTGCT GAAGATATTC TGGACAAGTA CCGGAATGCC 
ATTAAACGGA CCAGCCCCAG TGAAGGAGCA ATGGCAAATG ATGAGAGTGC AGAGGTCATG 
GGTGATGGTG AAAGTGCCCA CGACTCTCCT CGTGAGGAAG CACTGCAGAA CATCTCAGCC 
GACGACCTCC CGGACTCCGC CAGCCAGGCA GCCCACCCTC AGGACTCTGC TTTCTCTTAC 
AGAGATGTAA AAAAGAAGCT GAGGCTCGCA CTTTGCTCTG CAGACTCTGT GGCCTTCCCA 
GTGCTAACTC ATTCAACAAG GAATGGATTG CCAGACCACA CAGACCCAGA AGACAATGAA 
ATTGTATGCT TCTTAAAAGT TCAAATAGCT GAAGCAATTA ATTTACAAGA TAAGAGCTTA 
ATGGCTCAAC TTCAAGAAAC AATGCGTTGT GTGTGCCGCT TTGACAATAG GACTTGTAGG 
AAGTTGCTGG CATCCATTGC TGAAGACTAC AGAAAAAGAG CTCCGTATAT TGCTTATCTT 
ACCCGCTGCC GACAAGGACT GCAGACCACA CAGGCTCACT TGGAAAGGCT CTTGCAAAGA 
GTTTTGCGGG ACAAAGAGGT GGCCAATCGG TACTTCACCA CTGTCTGTGT GCGGTTACTG 
CTTGAGAGCA AAGAAAAGAA AATCAGGGAG TTCATTCAAG ACTTTCAGAA ACTTACTGCT 
GCTGATGATA AAACGGCTCA GGTGGAGGAT TTTCTACAGT TCCTGTATGG TGTGATGGCT 
CAGGATGTCA TATGGCAGAA TGCCAGTGAG GAGCAGCTGC AGGATGCCCA GCTGGCCATC 
GAGCGGAGTG TGATGAATCG GATCTTCAAG CTTGCTTTCT ACCCTAACCA GGATGGGGAC 
ATACTTCGTG ACCAGGTTCT GCATGAACAT ATCCAGAGAT TATCTAAAGT AGTGACTGCA 
AACCACAGAG CTCTGCAGAT CCCTGAGGTT TATCTCCGTG AGGCACCATG GCCATCTGCA 
CAGTCAGAAA TCAGGACAAT AAGTGCTTAC AAAACGCCCC GCGACAAAGT GCAGTGCATC 
CTGAGGATGT GCTCCACCAT CATGAACCTC TTGAGCCTGG CCAACGAGGA CTCTGTCCCC 
GGGGCAGATG ACTTTGTTCC TGTGTTGGTG TTTGTGTTAA TAAAGGCAAA CCCACCCTGC 
CTGCTGTCCA CTGTGCAGTA CATCAGCAGC TTCTATGCCA GCTGTCTGTC TGGAGAGGAG 
TCCTACTGGT GGATGCAGTT CACAGCAGCC GTGGAGTTTA TTAAAACCAT CGATGACAGA 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017174686.1
CDS	2614..5079
Translation	MMGLTDDRDISETVSETWSTDVLGSDFDPNVDEDRLQEIAGAAAENVLGSLLCLPGSGSVLLDPCTGSTISETTSEAWSVEVLPSDSEAPDLKQEERLQELESCSGLGSTSDDTDVREVSSRPSTPGLSVVSGISATSEDIPNKIEDLRSECSSDFGGKDSVTSPDMDDIAHGAHQLTSPPSQSESLLAMFDPLSSHEGASAVVRPKVHYARPSHPPPDPPILEGAVGGNEARLPNFGSHVLTAAEMEAFKQRHSYPERLVRSRSSDIVSSVRRPMSDPSWNRRPGNEELPPAAATGATSLVAAPHSSSSSPSKDSSRGETEERKDSDDERSDRSRPWWRKRFVSAMPKAPIPFRKKEKQEKDKDDLGPDRFSTLTDEPSPRLSAQAQVAEDILDKYRNAIKRTSPSEGAMANDESAEVMGDGESAHDSPREEALQNISADDLPDSASQAAHPQDSAFSYRDVKKKLRLALCSADSVAFPVLTHSTRNGLPDHTDPEDNEIVCFLKVQIAEAINLQDKSLMAQLQETMRCVCRFDNRTCRKLLASIAEDYRKRAPYIAYLTRCRQGLQTTQAHLERLLQRVLRDKEVANRYFTTVCVRLLLESKEKKIREFIQDFQKLTAADDKTAQVEDFLQFLYGVMAQDVIWQNASEEQLQDAQLAIERSVMNRIFKLAFYPNQDGDILRDQVLHEHIQRLSKVVTANHRALQIPEVYLREAPWPSAQSEIRTISAYKTPRDKVQCILRMCSTIMNLLSLANEDSVPGADDFVPVLVFVLIKANPPCLLSTVQYISSFYASCLSGEESYWWMQFTAAVEFIKTIDDRK

Target ORF information:

RefSeq Version	XM_017319197.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus GTPase activating protein and VPS9 domains 1 (Gapvd1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319197.1

ORF Insert Sequence:

ATGATGGGAC TAACAGATGA CAGGGACATA TCCGAGACTG TGAGTGAGAC CTGGAGCACT 
GATGTCCTGG GAAGTGACTT CGACCCGAAC GTTGATGAAG ATCGTTTGCA AGAGATTGCA 
GGTGCAGCAG CAGAGAACGT GTTAGGTAGT TTGCTGTGCC TTCCAGGTTC AGGGTCAGTT 
CTCCTTGACC CTTGCACTGG CTCTACCATA TCAGAGACCA CAAGTGAAGC TTGGAGTGTA 
GAGGTATTGC CAAGTGACTC AGAGGCTCCA GACTTAAAGC AGGAAGAGCG CCTCCAAGAG 
CTGGAGAGCT GTTCTGGACT GGGCAGCACA TCTGATGATA CGGATGTCAG GGAGGTCAGT 
TCCCGCCCCA GCACACCAGG CCTCAGTGTT GTGTCCGGTA TAAGTGCTAC ATCGGAGGAT 
ATTCCCAATA AGATCGAAGA CCTGAGATCT GAGTGCAGCT CTGATTTTGG GGGCAAAGAT 
TCTGTAACTA GTCCAGATAT GGATGACATA GCCCATGGCG CTCATCAGCT GACCTCTCCT 
CCCTCTCAGT CAGAGTCTCT GCTTGCCATG TTTGATCCAC TGTCCTCACA TGAAGGGGCC 
TCTGCTGTGG TAAGGCCAAA GGTTCACTAT GCCCGACCCT CGCATCCACC TCCGGATCCT 
CCAATCCTGG AAGGTGCAGT GGGAGGAAAT GAGGCCAGGT TGCCAAACTT TGGCTCTCAC 
GTTTTAACTG CAGCTGAAAT GGAGGCGTTC AAGCAGAGAC ACTCATACCC TGAGAGATTG 
GTTCGCAGCA GAAGCTCTGA TATAGTGTCT TCTGTGCGGC GGCCCATGAG TGACCCCAGC 
TGGAACCGAC GTCCAGGGAA TGAAGAACTT CCTCCAGCTG CAGCCACTGG TGCTACTTCT 
TTGGTGGCTG CACCTCACTC GTCATCTTCG TCCCCGAGTA AGGACTCTTC TAGAGGAGAG 
ACTGAGGAAC GCAAGGACAG TGACGATGAG AGGTCAGACA GGAGCAGGCC TTGGTGGAGG 
AAGCGCTTTG TTTCAGCTAT GCCTAAAGCT CCTATACCAT TTAGAAAGAA AGAAAAACAA 
GAAAAAGACA AAGATGATCT GGGTCCTGAC AGATTCTCAA CACTCACAGA TGAGCCCAGC 
CCTAGGCTCA GTGCTCAAGC TCAGGTTGCT GAAGATATTC TGGACAAGTA CCGGAATGCC 
ATTAAACGGA CCAGCCCCAG TGAAGGAGCA ATGGCAAATG ATGAGAGTGC AGAGGTCATG 
GGTGATGGTG AAAGTGCCCA CGACTCTCCT CGTGAGGAAG CACTGCAGAA CATCTCAGCC 
GACGACCTCC CGGACTCCGC CAGCCAGGCA GCCCACCCTC AGGACTCTGC TTTCTCTTAC 
AGAGATGTAA AAAAGAAGCT GAGGCTCGCA CTTTGCTCTG CAGACTCTGT GGCCTTCCCA 
GTGCTAACTC ATTCAACAAG GAATGGATTG CCAGACCACA CAGACCCAGA AGACAATGAA 
ATTGTATGCT TCTTAAAAGT TCAAATAGCT GAAGCAATTA ATTTACAAGA TAAGAGCTTA 
ATGGCTCAAC TTCAAGAAAC AATGCGTTGT GTGTGCCGCT TTGACAATAG GACTTGTAGG 
AAGTTGCTGG CATCCATTGC TGAAGACTAC AGAAAAAGAG CTCCGTATAT TGCTTATCTT 
ACCCGCTGCC GACAAGGACT GCAGACCACA CAGGCTCACT TGGAAAGGCT CTTGCAAAGA 
GTTTTGCGGG ACAAAGAGGT GGCCAATCGG TACTTCACCA CTGTCTGTGT GCGGTTACTG 
CTTGAGAGCA AAGAAAAGAA AATCAGGGAG TTCATTCAAG ACTTTCAGAA ACTTACTGCT 
GCTGATGATA AAACGGCTCA GGTGGAGGAT TTTCTACAGT TCCTGTATGG TGTGATGGCT 
CAGGATGTCA TATGGCAGAA TGCCAGTGAG GAGCAGCTGC AGGATGCCCA GCTGGCCATC 
GAGCGGAGTG TGATGAATCG GATCTTCAAG CTTGCTTTCT ACCCTAACCA GGATGGGGAC 
ATACTTCGTG ACCAGGTTCT GCATGAACAT ATCCAGAGAT TATCTAAAGT AGTGACTGCA 
AACCACAGAG CTCTGCAGAT CCCTGAGGTT TATCTCCGTG AGGCACCATG GCCATCTGCA 
CAGTCAGAAA TCAGGACAAT AAGTGCTTAC AAAACGCCCC GCGACAAAGT GCAGTGCATC 
CTGAGGATGT GCTCCACCAT CATGAACCTC TTGAGCCTGG CCAACGAGGA CTCTGTCCCC 
GGGGCAGATG ACTTTGTTCC TGTGTTGGTG TTTGTGTTAA TAAAGGCAAA CCCACCCTGC 
CTGCTGTCCA CTGTGCAGTA CATCAGCAGC TTCTATGCCA GCTGTCTGTC TGGAGAGGAG 
TCCTACTGGT GGATGCAGTT CACAGCAGCC GTGGAGTTTA TTAAAACCAT CGATGACAGA 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAPVD1 ( XM_017319199.1 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_017174688.1 Mus musculus GTPase activating protein and VPS9 domains 1 (Gapvd1), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OMu80384
Clone ID Related Accession (Same CDS sequence)	XM_017319199.1
Accession Version	XM_017319199.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	GTPase-activating protein and VPS9 domain-containing protein 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGACA TAGCCCATGG CGCTCATCAG CTGACCTCTC CTCCCTCTCA GTCAGAGTCT 
CTGCTTGCCA TGTTTGATCC ACTGTCCTCA CATGAAGGGG CCTCTGCTGT GGTAAGGCCA 
AAGGTTCACT ATGCCCGACC CTCGCATCCA CCTCCGGATC CTCCAATCCT GGAAGGTGCA 
GTGGGAGGAA ATGAGGCCAG GTTGCCAAAC TTTGGCTCTC ACGTTTTAAC TGCAGCTGAA 
ATGGAGGCGT TCAAGCAGAG ACACTCATAC CCTGAGAGAT TGGTTCGCAG CAGAAGCTCT 
GATATAGTGT CTTCTGTGCG GCGGCCCATG AGTGACCCCA GCTGGAACCG ACGTCCAGGG 
AATGAAGAAC TTCCTCCAGC TGCAGCCACT GGTGCTACTT CTTTGGTGGC TGCACCTCAC 
TCGTCATCTT CGTCCCCGAG TAAGGACTCT TCTAGAGGAG AGACTGAGGA ACGCAAGGAC 
AGTGACGATG AGAGGTCAGA CAGGAGCAGG CCTTGGTGGA GGAAGCGCTT TGTTTCAGCT 
ATGCCTAAAG CTCCTATACC ATTTAGAAAG AAAGAAAAAC AAGAAAAAGA CAAAGATGAT 
CTGGGTCCTG ACAGATTCTC AACACTCACA GATGAGCCCA GCCCTAGGCT CAGTGCTCAA 
GCTCAGGTTG CTGAAGATAT TCTGGACAAG TACCGGAATG CCATTAAACG GACCAGCCCC 
AGTGAAGGAG CAATGGCAAA TGATGAGAGT GCAGAGGTCA TGGGTGATGG TGAAAGTGCC 
CACGACTCTC CTCGTGAGGA AGCACTGCAG AACATCTCAG CCGACGACCT CCCGGACTCC 
GCCAGCCAGG CAGCCCACCC TCAGGACTCT GCTTTCTCTT ACAGAGATGT AAAAAAGAAG 
CTGAGGCTCG CACTTTGCTC TGCAGACTCT GTGGCCTTCC CAGTGCTAAC TCATTCAACA 
AGGAATGGAT TGCCAGACCA CACAGACCCA GAAGACAATG AAATTGTATG CTTCTTAAAA 
GTTCAAATAG CTGAAGCAAT TAATTTACAA GATAAGAGCT TAATGGCTCA ACTTCAAGAA 
ACAATGCGTT GTGTGTGCCG CTTTGACAAT AGGACTTGTA GGAAGTTGCT GGCATCCATT 
GCTGAAGACT ACAGAAAAAG AGCTCCGTAT ATTGCTTATC TTACCCGCTG CCGACAAGGA 
CTGCAGACCA CACAGGCTCA CTTGGAAAGG CTCTTGCAAA GAGTTTTGCG GGACAAAGAG 
GTGGCCAATC GGTACTTCAC CACTGTCTGT GTGCGGTTAC TGCTTGAGAG CAAAGAAAAG 
AAAATCAGGG AGTTCATTCA AGACTTTCAG AAACTTACTG CTGCTGATGA TAAAACGGCT 
CAGGTGGAGG ATTTTCTACA GTTCCTGTAT GGTGTGATGG CTCAGGATGT CATATGGCAG 
AATGCCAGTG AGGAGCAGCT GCAGGATGCC CAGCTGGCCA TCGAGCGGAG TGTGATGAAT 
CGGATCTTCA AGCTTGCTTT CTACCCTAAC CAGGATGGGG ACATACTTCG TGACCAGGTT 
CTGCATGAAC ATATCCAGAG ATTATCTAAA GTAGTGACTG CAAACCACAG AGCTCTGCAG 
ATCCCTGAGG TTTATCTCCG TGAGGCACCA TGGCCATCTG CACAGTCAGA AATCAGGACA 
ATAAGTGCTT ACAAAACGCC CCGCGACAAA GTGCAGTGCA TCCTGAGGAT GTGCTCCACC 
ATCATGAACC TCTTGAGCCT GGCCAACGAG GACTCTGTCC CCGGGGCAGA TGACTTTGTT 
CCTGTGTTGG TGTTTGTGTT AATAAAGGCA AACCCACCCT GCCTGCTGTC CACTGTGCAG 
TACATCAGCA GCTTCTATGC CAGCTGTCTG TCTGGAGAGG AGTCCTACTG GTGGATGCAG 
TTCACAGCAG CCGTGGAGTT TATTAAAACC ATCGATGACA GAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017174688.1
CDS	389..2356
Translation	MDDIAHGAHQLTSPPSQSESLLAMFDPLSSHEGASAVVRPKVHYARPSHPPPDPPILEGAVGGNEARLPNFGSHVLTAAEMEAFKQRHSYPERLVRSRSSDIVSSVRRPMSDPSWNRRPGNEELPPAAATGATSLVAAPHSSSSSPSKDSSRGETEERKDSDDERSDRSRPWWRKRFVSAMPKAPIPFRKKEKQEKDKDDLGPDRFSTLTDEPSPRLSAQAQVAEDILDKYRNAIKRTSPSEGAMANDESAEVMGDGESAHDSPREEALQNISADDLPDSASQAAHPQDSAFSYRDVKKKLRLALCSADSVAFPVLTHSTRNGLPDHTDPEDNEIVCFLKVQIAEAINLQDKSLMAQLQETMRCVCRFDNRTCRKLLASIAEDYRKRAPYIAYLTRCRQGLQTTQAHLERLLQRVLRDKEVANRYFTTVCVRLLLESKEKKIREFIQDFQKLTAADDKTAQVEDFLQFLYGVMAQDVIWQNASEEQLQDAQLAIERSVMNRIFKLAFYPNQDGDILRDQVLHEHIQRLSKVVTANHRALQIPEVYLREAPWPSAQSEIRTISAYKTPRDKVQCILRMCSTIMNLLSLANEDSVPGADDFVPVLVFVLIKANPPCLLSTVQYISSFYASCLSGEESYWWMQFTAAVEFIKTIDDRK

Target ORF information:

RefSeq Version	XM_017319199.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus GTPase activating protein and VPS9 domains 1 (Gapvd1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319199.1

ORF Insert Sequence:

ATGGATGACA TAGCCCATGG CGCTCATCAG CTGACCTCTC CTCCCTCTCA GTCAGAGTCT 
CTGCTTGCCA TGTTTGATCC ACTGTCCTCA CATGAAGGGG CCTCTGCTGT GGTAAGGCCA 
AAGGTTCACT ATGCCCGACC CTCGCATCCA CCTCCGGATC CTCCAATCCT GGAAGGTGCA 
GTGGGAGGAA ATGAGGCCAG GTTGCCAAAC TTTGGCTCTC ACGTTTTAAC TGCAGCTGAA 
ATGGAGGCGT TCAAGCAGAG ACACTCATAC CCTGAGAGAT TGGTTCGCAG CAGAAGCTCT 
GATATAGTGT CTTCTGTGCG GCGGCCCATG AGTGACCCCA GCTGGAACCG ACGTCCAGGG 
AATGAAGAAC TTCCTCCAGC TGCAGCCACT GGTGCTACTT CTTTGGTGGC TGCACCTCAC 
TCGTCATCTT CGTCCCCGAG TAAGGACTCT TCTAGAGGAG AGACTGAGGA ACGCAAGGAC 
AGTGACGATG AGAGGTCAGA CAGGAGCAGG CCTTGGTGGA GGAAGCGCTT TGTTTCAGCT 
ATGCCTAAAG CTCCTATACC ATTTAGAAAG AAAGAAAAAC AAGAAAAAGA CAAAGATGAT 
CTGGGTCCTG ACAGATTCTC AACACTCACA GATGAGCCCA GCCCTAGGCT CAGTGCTCAA 
GCTCAGGTTG CTGAAGATAT TCTGGACAAG TACCGGAATG CCATTAAACG GACCAGCCCC 
AGTGAAGGAG CAATGGCAAA TGATGAGAGT GCAGAGGTCA TGGGTGATGG TGAAAGTGCC 
CACGACTCTC CTCGTGAGGA AGCACTGCAG AACATCTCAG CCGACGACCT CCCGGACTCC 
GCCAGCCAGG CAGCCCACCC TCAGGACTCT GCTTTCTCTT ACAGAGATGT AAAAAAGAAG 
CTGAGGCTCG CACTTTGCTC TGCAGACTCT GTGGCCTTCC CAGTGCTAAC TCATTCAACA 
AGGAATGGAT TGCCAGACCA CACAGACCCA GAAGACAATG AAATTGTATG CTTCTTAAAA 
GTTCAAATAG CTGAAGCAAT TAATTTACAA GATAAGAGCT TAATGGCTCA ACTTCAAGAA 
ACAATGCGTT GTGTGTGCCG CTTTGACAAT AGGACTTGTA GGAAGTTGCT GGCATCCATT 
GCTGAAGACT ACAGAAAAAG AGCTCCGTAT ATTGCTTATC TTACCCGCTG CCGACAAGGA 
CTGCAGACCA CACAGGCTCA CTTGGAAAGG CTCTTGCAAA GAGTTTTGCG GGACAAAGAG 
GTGGCCAATC GGTACTTCAC CACTGTCTGT GTGCGGTTAC TGCTTGAGAG CAAAGAAAAG 
AAAATCAGGG AGTTCATTCA AGACTTTCAG AAACTTACTG CTGCTGATGA TAAAACGGCT 
CAGGTGGAGG ATTTTCTACA GTTCCTGTAT GGTGTGATGG CTCAGGATGT CATATGGCAG 
AATGCCAGTG AGGAGCAGCT GCAGGATGCC CAGCTGGCCA TCGAGCGGAG TGTGATGAAT 
CGGATCTTCA AGCTTGCTTT CTACCCTAAC CAGGATGGGG ACATACTTCG TGACCAGGTT 
CTGCATGAAC ATATCCAGAG ATTATCTAAA GTAGTGACTG CAAACCACAG AGCTCTGCAG 
ATCCCTGAGG TTTATCTCCG TGAGGCACCA TGGCCATCTG CACAGTCAGA AATCAGGACA 
ATAAGTGCTT ACAAAACGCC CCGCGACAAA GTGCAGTGCA TCCTGAGGAT GTGCTCCACC 
ATCATGAACC TCTTGAGCCT GGCCAACGAG GACTCTGTCC CCGGGGCAGA TGACTTTGTT 
CCTGTGTTGG TGTTTGTGTT AATAAAGGCA AACCCACCCT GCCTGCTGTC CACTGTGCAG 
TACATCAGCA GCTTCTATGC CAGCTGTCTG TCTGGAGAGG AGTCCTACTG GTGGATGCAG 
TTCACAGCAG CCGTGGAGTT TATTAAAACC ATCGATGACA GAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAPVD1 ( XM_017319197.2 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_017174686.1 Mus musculus GTPase activating protein and VPS9 domains 1 (Gapvd1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OMu80382
Clone ID Related Accession (Same CDS sequence)	XM_017319197.2 , XM_017319197.1
Accession Version	XM_017319197.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2466bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	GTPase-activating protein and VPS9 domain-containing protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017319197.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGGAC TAACAGATGA CAGGGACATA TCCGAGACTG TGAGTGAGAC CTGGAGCACT 
GATGTCCTGG GAAGTGACTT CGACCCGAAC GTTGATGAAG ATCGTTTGCA AGAGATTGCA 
GGTGCAGCAG CAGAGAACGT GTTAGGTAGT TTGCTGTGCC TTCCAGGTTC AGGGTCAGTT 
CTCCTTGACC CTTGCACTGG CTCTACCATA TCAGAGACCA CAAGTGAAGC TTGGAGTGTA 
GAGGTATTGC CAAGTGACTC AGAGGCTCCA GACTTAAAGC AGGAAGAGCG CCTCCAAGAG 
CTGGAGAGCT GTTCTGGACT GGGCAGCACA TCTGATGATA CGGATGTCAG GGAGGTCAGT 
TCCCGCCCCA GCACACCAGG CCTCAGTGTT GTGTCCGGTA TAAGTGCTAC ATCGGAGGAT 
ATTCCCAATA AGATCGAAGA CCTGAGATCT GAGTGCAGCT CTGATTTTGG GGGCAAAGAT 
TCTGTAACTA GTCCAGATAT GGATGACATA GCCCATGGCG CTCATCAGCT GACCTCTCCT 
CCCTCTCAGT CAGAGTCTCT GCTTGCCATG TTTGATCCAC TGTCCTCACA TGAAGGGGCC 
TCTGCTGTGG TAAGGCCAAA GGTTCACTAT GCCCGACCCT CGCATCCACC TCCGGATCCT 
CCAATCCTGG AAGGTGCAGT GGGAGGAAAT GAGGCCAGGT TGCCAAACTT TGGCTCTCAC 
GTTTTAACTG CAGCTGAAAT GGAGGCGTTC AAGCAGAGAC ACTCATACCC TGAGAGATTG 
GTTCGCAGCA GAAGCTCTGA TATAGTGTCT TCTGTGCGGC GGCCCATGAG TGACCCCAGC 
TGGAACCGAC GTCCAGGGAA TGAAGAACTT CCTCCAGCTG CAGCCACTGG TGCTACTTCT 
TTGGTGGCTG CACCTCACTC GTCATCTTCG TCCCCGAGTA AGGACTCTTC TAGAGGAGAG 
ACTGAGGAAC GCAAGGACAG TGACGATGAG AGGTCAGACA GGAGCAGGCC TTGGTGGAGG 
AAGCGCTTTG TTTCAGCTAT GCCTAAAGCT CCTATACCAT TTAGAAAGAA AGAAAAACAA 
GAAAAAGACA AAGATGATCT GGGTCCTGAC AGATTCTCAA CACTCACAGA TGAGCCCAGC 
CCTAGGCTCA GTGCTCAAGC TCAGGTTGCT GAAGATATTC TGGACAAGTA CCGGAATGCC 
ATTAAACGGA CCAGCCCCAG TGAAGGAGCA ATGGCAAATG ATGAGAGTGC AGAGGTCATG 
GGTGATGGTG AAAGTGCCCA CGACTCTCCT CGTGAGGAAG CACTGCAGAA CATCTCAGCC 
GACGACCTCC CGGACTCCGC CAGCCAGGCA GCCCACCCTC AGGACTCTGC TTTCTCTTAC 
AGAGATGTAA AAAAGAAGCT GAGGCTCGCA CTTTGCTCTG CAGACTCTGT GGCCTTCCCA 
GTGCTAACTC ATTCAACAAG GAATGGATTG CCAGACCACA CAGACCCAGA AGACAATGAA 
ATTGTATGCT TCTTAAAAGT TCAAATAGCT GAAGCAATTA ATTTACAAGA TAAGAGCTTA 
ATGGCTCAAC TTCAAGAAAC AATGCGTTGT GTGTGCCGCT TTGACAATAG GACTTGTAGG 
AAGTTGCTGG CATCCATTGC TGAAGACTAC AGAAAAAGAG CTCCGTATAT TGCTTATCTT 
ACCCGCTGCC GACAAGGACT GCAGACCACA CAGGCTCACT TGGAAAGGCT CTTGCAAAGA 
GTTTTGCGGG ACAAAGAGGT GGCCAATCGG TACTTCACCA CTGTCTGTGT GCGGTTACTG 
CTTGAGAGCA AAGAAAAGAA AATCAGGGAG TTCATTCAAG ACTTTCAGAA ACTTACTGCT 
GCTGATGATA AAACGGCTCA GGTGGAGGAT TTTCTACAGT TCCTGTATGG TGTGATGGCT 
CAGGATGTCA TATGGCAGAA TGCCAGTGAG GAGCAGCTGC AGGATGCCCA GCTGGCCATC 
GAGCGGAGTG TGATGAATCG GATCTTCAAG CTTGCTTTCT ACCCTAACCA GGATGGGGAC 
ATACTTCGTG ACCAGGTTCT GCATGAACAT ATCCAGAGAT TATCTAAAGT AGTGACTGCA 
AACCACAGAG CTCTGCAGAT CCCTGAGGTT TATCTCCGTG AGGCACCATG GCCATCTGCA 
CAGTCAGAAA TCAGGACAAT AAGTGCTTAC AAAACGCCCC GCGACAAAGT GCAGTGCATC 
CTGAGGATGT GCTCCACCAT CATGAACCTC TTGAGCCTGG CCAACGAGGA CTCTGTCCCC 
GGGGCAGATG ACTTTGTTCC TGTGTTGGTG TTTGTGTTAA TAAAGGCAAA CCCACCCTGC 
CTGCTGTCCA CTGTGCAGTA CATCAGCAGC TTCTATGCCA GCTGTCTGTC TGGAGAGGAG 
TCCTACTGGT GGATGCAGTT CACAGCAGCC GTGGAGTTTA TTAAAACCAT CGATGACAGA 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017174686.1
CDS	2614..5079
Translation	MMGLTDDRDISETVSETWSTDVLGSDFDPNVDEDRLQEIAGAAAENVLGSLLCLPGSGSVLLDPCTGSTISETTSEAWSVEVLPSDSEAPDLKQEERLQELESCSGLGSTSDDTDVREVSSRPSTPGLSVVSGISATSEDIPNKIEDLRSECSSDFGGKDSVTSPDMDDIAHGAHQLTSPPSQSESLLAMFDPLSSHEGASAVVRPKVHYARPSHPPPDPPILEGAVGGNEARLPNFGSHVLTAAEMEAFKQRHSYPERLVRSRSSDIVSSVRRPMSDPSWNRRPGNEELPPAAATGATSLVAAPHSSSSSPSKDSSRGETEERKDSDDERSDRSRPWWRKRFVSAMPKAPIPFRKKEKQEKDKDDLGPDRFSTLTDEPSPRLSAQAQVAEDILDKYRNAIKRTSPSEGAMANDESAEVMGDGESAHDSPREEALQNISADDLPDSASQAAHPQDSAFSYRDVKKKLRLALCSADSVAFPVLTHSTRNGLPDHTDPEDNEIVCFLKVQIAEAINLQDKSLMAQLQETMRCVCRFDNRTCRKLLASIAEDYRKRAPYIAYLTRCRQGLQTTQAHLERLLQRVLRDKEVANRYFTTVCVRLLLESKEKKIREFIQDFQKLTAADDKTAQVEDFLQFLYGVMAQDVIWQNASEEQLQDAQLAIERSVMNRIFKLAFYPNQDGDILRDQVLHEHIQRLSKVVTANHRALQIPEVYLREAPWPSAQSEIRTISAYKTPRDKVQCILRMCSTIMNLLSLANEDSVPGADDFVPVLVFVLIKANPPCLLSTVQYISSFYASCLSGEESYWWMQFTAAVEFIKTIDDRK

Target ORF information:

RefSeq Version	XM_017319197.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus GTPase activating protein and VPS9 domains 1 (Gapvd1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319197.2

ORF Insert Sequence:

ATGATGGGAC TAACAGATGA CAGGGACATA TCCGAGACTG TGAGTGAGAC CTGGAGCACT 
GATGTCCTGG GAAGTGACTT CGACCCGAAC GTTGATGAAG ATCGTTTGCA AGAGATTGCA 
GGTGCAGCAG CAGAGAACGT GTTAGGTAGT TTGCTGTGCC TTCCAGGTTC AGGGTCAGTT 
CTCCTTGACC CTTGCACTGG CTCTACCATA TCAGAGACCA CAAGTGAAGC TTGGAGTGTA 
GAGGTATTGC CAAGTGACTC AGAGGCTCCA GACTTAAAGC AGGAAGAGCG CCTCCAAGAG 
CTGGAGAGCT GTTCTGGACT GGGCAGCACA TCTGATGATA CGGATGTCAG GGAGGTCAGT 
TCCCGCCCCA GCACACCAGG CCTCAGTGTT GTGTCCGGTA TAAGTGCTAC ATCGGAGGAT 
ATTCCCAATA AGATCGAAGA CCTGAGATCT GAGTGCAGCT CTGATTTTGG GGGCAAAGAT 
TCTGTAACTA GTCCAGATAT GGATGACATA GCCCATGGCG CTCATCAGCT GACCTCTCCT 
CCCTCTCAGT CAGAGTCTCT GCTTGCCATG TTTGATCCAC TGTCCTCACA TGAAGGGGCC 
TCTGCTGTGG TAAGGCCAAA GGTTCACTAT GCCCGACCCT CGCATCCACC TCCGGATCCT 
CCAATCCTGG AAGGTGCAGT GGGAGGAAAT GAGGCCAGGT TGCCAAACTT TGGCTCTCAC 
GTTTTAACTG CAGCTGAAAT GGAGGCGTTC AAGCAGAGAC ACTCATACCC TGAGAGATTG 
GTTCGCAGCA GAAGCTCTGA TATAGTGTCT TCTGTGCGGC GGCCCATGAG TGACCCCAGC 
TGGAACCGAC GTCCAGGGAA TGAAGAACTT CCTCCAGCTG CAGCCACTGG TGCTACTTCT 
TTGGTGGCTG CACCTCACTC GTCATCTTCG TCCCCGAGTA AGGACTCTTC TAGAGGAGAG 
ACTGAGGAAC GCAAGGACAG TGACGATGAG AGGTCAGACA GGAGCAGGCC TTGGTGGAGG 
AAGCGCTTTG TTTCAGCTAT GCCTAAAGCT CCTATACCAT TTAGAAAGAA AGAAAAACAA 
GAAAAAGACA AAGATGATCT GGGTCCTGAC AGATTCTCAA CACTCACAGA TGAGCCCAGC 
CCTAGGCTCA GTGCTCAAGC TCAGGTTGCT GAAGATATTC TGGACAAGTA CCGGAATGCC 
ATTAAACGGA CCAGCCCCAG TGAAGGAGCA ATGGCAAATG ATGAGAGTGC AGAGGTCATG 
GGTGATGGTG AAAGTGCCCA CGACTCTCCT CGTGAGGAAG CACTGCAGAA CATCTCAGCC 
GACGACCTCC CGGACTCCGC CAGCCAGGCA GCCCACCCTC AGGACTCTGC TTTCTCTTAC 
AGAGATGTAA AAAAGAAGCT GAGGCTCGCA CTTTGCTCTG CAGACTCTGT GGCCTTCCCA 
GTGCTAACTC ATTCAACAAG GAATGGATTG CCAGACCACA CAGACCCAGA AGACAATGAA 
ATTGTATGCT TCTTAAAAGT TCAAATAGCT GAAGCAATTA ATTTACAAGA TAAGAGCTTA 
ATGGCTCAAC TTCAAGAAAC AATGCGTTGT GTGTGCCGCT TTGACAATAG GACTTGTAGG 
AAGTTGCTGG CATCCATTGC TGAAGACTAC AGAAAAAGAG CTCCGTATAT TGCTTATCTT 
ACCCGCTGCC GACAAGGACT GCAGACCACA CAGGCTCACT TGGAAAGGCT CTTGCAAAGA 
GTTTTGCGGG ACAAAGAGGT GGCCAATCGG TACTTCACCA CTGTCTGTGT GCGGTTACTG 
CTTGAGAGCA AAGAAAAGAA AATCAGGGAG TTCATTCAAG ACTTTCAGAA ACTTACTGCT 
GCTGATGATA AAACGGCTCA GGTGGAGGAT TTTCTACAGT TCCTGTATGG TGTGATGGCT 
CAGGATGTCA TATGGCAGAA TGCCAGTGAG GAGCAGCTGC AGGATGCCCA GCTGGCCATC 
GAGCGGAGTG TGATGAATCG GATCTTCAAG CTTGCTTTCT ACCCTAACCA GGATGGGGAC 
ATACTTCGTG ACCAGGTTCT GCATGAACAT ATCCAGAGAT TATCTAAAGT AGTGACTGCA 
AACCACAGAG CTCTGCAGAT CCCTGAGGTT TATCTCCGTG AGGCACCATG GCCATCTGCA 
CAGTCAGAAA TCAGGACAAT AAGTGCTTAC AAAACGCCCC GCGACAAAGT GCAGTGCATC 
CTGAGGATGT GCTCCACCAT CATGAACCTC TTGAGCCTGG CCAACGAGGA CTCTGTCCCC 
GGGGCAGATG ACTTTGTTCC TGTGTTGGTG TTTGTGTTAA TAAAGGCAAA CCCACCCTGC 
CTGCTGTCCA CTGTGCAGTA CATCAGCAGC TTCTATGCCA GCTGTCTGTC TGGAGAGGAG 
TCCTACTGGT GGATGCAGTT CACAGCAGCC GTGGAGTTTA TTAAAACCAT CGATGACAGA 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAPVD1 ( XM_017319198.1 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_017174687.1 Mus musculus GTPase activating protein and VPS9 domains 1 (Gapvd1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OMu80383
Clone ID Related Accession (Same CDS sequence)	XM_017319198.1
Accession Version	XM_017319198.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2385bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	GTPase-activating protein and VPS9 domain-containing protein 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGGAC TAACAGATGA CAGGGACATA TCCGAGACTG TGAGTGAGAC CTGGAGCACT 
GATGTCCTGG GAAGTGACTT CGACCCGAAC GTTGATGAAG ATCGTTTGCA AGAGATTGCA 
GGTGCAGCAG CAGAGAACGT GTTAGGTAGT TTGCTGTGCC TTCCAGGTTC AGGGTCAGTT 
CTCCTTGACC CTTGCACTGG CTCTACCATA TCAGAGACCA CAAGTGAAGC TTGGAGTGTA 
GAGGTATTGC CAAGTGACTC AGAGGCTCCA GACTTAAAGC AGGAAGAGCG CCTCCAAGAG 
CTGGAGAGCT GTTCTGGACT GGGCAGCACA TCTGATGATA CGGATGTCAG GGAGGTCAGT 
TCCCGCCCCA GCACACCAGG CCTCAGTGTT GTGTCCGGTA TAAGTGCTAC ATCGGAGGAT 
ATTCCCAATA AGATCGAAGA CCTGAGATCT GAGTGCAGCT CTGATTTTGG GGGCAAAGAT 
TCTGTAACTA GTCCAGATAT GGATGACATA GCCCATGGCG CTCATCAGCT GACCTCTCCT 
CCCTCTCAGT CAGAGTCTCT GCTTGCCATG TTTGATCCAC TGTCCTCACA TGAAGGGGCC 
TCTGCTGTGG TAAGGCCAAA GGTTCACTAT GCCCGACCCT CGCATCCACC TCCGGATCCT 
CCAATCCTGG AAGGTGCAGT GGGAGGAAAT GAGGCCAGGT TGCCAAACTT TGGCTCTCAC 
GTTTTAACTG CAGCTGAAAT GGAGGCGTTC AAGCAGAGAC ACTCATACCC TGAGAGATTG 
GTTCGCAGCA GAAGCTCTGA TATAGTGTCT TCTGTGCGGC GGCCCATGAG TGACCCCAGC 
TGGAACCGAC GTCCAGGGAA TGAAGAACTT CCTCCAGCTG CAGCCACTGG TGCTACTTCT 
TTGGTGGCTG CACCTCACTC GTCATCTTCG TCCCCGAGTA AGGACTCTTC TAGAGGAGAG 
ACTGAGGAAC GCAAGGACAG TGACGATGAG AGGTCAGACA GGAGCAGGCC TTGGTGGAGG 
AAGCGCTTTG TTTCAGCTAT GCCTAAAGAT GAGCCCAGCC CTAGGCTCAG TGCTCAAGCT 
CAGGTTGCTG AAGATATTCT GGACAAGTAC CGGAATGCCA TTAAACGGAC CAGCCCCAGT 
GAAGGAGCAA TGGCAAATGA TGAGAGTGCA GAGGTCATGG GTGATGGTGA AAGTGCCCAC 
GACTCTCCTC GTGAGGAAGC ACTGCAGAAC ATCTCAGCCG ACGACCTCCC GGACTCCGCC 
AGCCAGGCAG CCCACCCTCA GGACTCTGCT TTCTCTTACA GAGATGTAAA AAAGAAGCTG 
AGGCTCGCAC TTTGCTCTGC AGACTCTGTG GCCTTCCCAG TGCTAACTCA TTCAACAAGG 
AATGGATTGC CAGACCACAC AGACCCAGAA GACAATGAAA TTGTATGCTT CTTAAAAGTT 
CAAATAGCTG AAGCAATTAA TTTACAAGAT AAGAGCTTAA TGGCTCAACT TCAAGAAACA 
ATGCGTTGTG TGTGCCGCTT TGACAATAGG ACTTGTAGGA AGTTGCTGGC ATCCATTGCT 
GAAGACTACA GAAAAAGAGC TCCGTATATT GCTTATCTTA CCCGCTGCCG ACAAGGACTG 
CAGACCACAC AGGCTCACTT GGAAAGGCTC TTGCAAAGAG TTTTGCGGGA CAAAGAGGTG 
GCCAATCGGT ACTTCACCAC TGTCTGTGTG CGGTTACTGC TTGAGAGCAA AGAAAAGAAA 
ATCAGGGAGT TCATTCAAGA CTTTCAGAAA CTTACTGCTG CTGATGATAA AACGGCTCAG 
GTGGAGGATT TTCTACAGTT CCTGTATGGT GTGATGGCTC AGGATGTCAT ATGGCAGAAT 
GCCAGTGAGG AGCAGCTGCA GGATGCCCAG CTGGCCATCG AGCGGAGTGT GATGAATCGG 
ATCTTCAAGC TTGCTTTCTA CCCTAACCAG GATGGGGACA TACTTCGTGA CCAGGTTCTG 
CATGAACATA TCCAGAGATT ATCTAAAGTA GTGACTGCAA ACCACAGAGC TCTGCAGATC 
CCTGAGGTTT ATCTCCGTGA GGCACCATGG CCATCTGCAC AGTCAGAAAT CAGGACAATA 
AGTGCTTACA AAACGCCCCG CGACAAAGTG CAGTGCATCC TGAGGATGTG CTCCACCATC 
ATGAACCTCT TGAGCCTGGC CAACGAGGAC TCTGTCCCCG GGGCAGATGA CTTTGTTCCT 
GTGTTGGTGT TTGTGTTAAT AAAGGCAAAC CCACCCTGCC TGCTGTCCAC TGTGCAGTAC 
ATCAGCAGCT TCTATGCCAG CTGTCTGTCT GGAGAGGAGT CCTACTGGTG GATGCAGTTC 
ACAGCAGCCG TGGAGTTTAT TAAAACCATC GATGACAGAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017174687.1
CDS	2416..4800
Translation	MMGLTDDRDISETVSETWSTDVLGSDFDPNVDEDRLQEIAGAAAENVLGSLLCLPGSGSVLLDPCTGSTISETTSEAWSVEVLPSDSEAPDLKQEERLQELESCSGLGSTSDDTDVREVSSRPSTPGLSVVSGISATSEDIPNKIEDLRSECSSDFGGKDSVTSPDMDDIAHGAHQLTSPPSQSESLLAMFDPLSSHEGASAVVRPKVHYARPSHPPPDPPILEGAVGGNEARLPNFGSHVLTAAEMEAFKQRHSYPERLVRSRSSDIVSSVRRPMSDPSWNRRPGNEELPPAAATGATSLVAAPHSSSSSPSKDSSRGETEERKDSDDERSDRSRPWWRKRFVSAMPKDEPSPRLSAQAQVAEDILDKYRNAIKRTSPSEGAMANDESAEVMGDGESAHDSPREEALQNISADDLPDSASQAAHPQDSAFSYRDVKKKLRLALCSADSVAFPVLTHSTRNGLPDHTDPEDNEIVCFLKVQIAEAINLQDKSLMAQLQETMRCVCRFDNRTCRKLLASIAEDYRKRAPYIAYLTRCRQGLQTTQAHLERLLQRVLRDKEVANRYFTTVCVRLLLESKEKKIREFIQDFQKLTAADDKTAQVEDFLQFLYGVMAQDVIWQNASEEQLQDAQLAIERSVMNRIFKLAFYPNQDGDILRDQVLHEHIQRLSKVVTANHRALQIPEVYLREAPWPSAQSEIRTISAYKTPRDKVQCILRMCSTIMNLLSLANEDSVPGADDFVPVLVFVLIKANPPCLLSTVQYISSFYASCLSGEESYWWMQFTAAVEFIKTIDDRK

Target ORF information:

RefSeq Version	XM_017319198.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus GTPase activating protein and VPS9 domains 1 (Gapvd1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319198.1

ORF Insert Sequence:

ATGATGGGAC TAACAGATGA CAGGGACATA TCCGAGACTG TGAGTGAGAC CTGGAGCACT 
GATGTCCTGG GAAGTGACTT CGACCCGAAC GTTGATGAAG ATCGTTTGCA AGAGATTGCA 
GGTGCAGCAG CAGAGAACGT GTTAGGTAGT TTGCTGTGCC TTCCAGGTTC AGGGTCAGTT 
CTCCTTGACC CTTGCACTGG CTCTACCATA TCAGAGACCA CAAGTGAAGC TTGGAGTGTA 
GAGGTATTGC CAAGTGACTC AGAGGCTCCA GACTTAAAGC AGGAAGAGCG CCTCCAAGAG 
CTGGAGAGCT GTTCTGGACT GGGCAGCACA TCTGATGATA CGGATGTCAG GGAGGTCAGT 
TCCCGCCCCA GCACACCAGG CCTCAGTGTT GTGTCCGGTA TAAGTGCTAC ATCGGAGGAT 
ATTCCCAATA AGATCGAAGA CCTGAGATCT GAGTGCAGCT CTGATTTTGG GGGCAAAGAT 
TCTGTAACTA GTCCAGATAT GGATGACATA GCCCATGGCG CTCATCAGCT GACCTCTCCT 
CCCTCTCAGT CAGAGTCTCT GCTTGCCATG TTTGATCCAC TGTCCTCACA TGAAGGGGCC 
TCTGCTGTGG TAAGGCCAAA GGTTCACTAT GCCCGACCCT CGCATCCACC TCCGGATCCT 
CCAATCCTGG AAGGTGCAGT GGGAGGAAAT GAGGCCAGGT TGCCAAACTT TGGCTCTCAC 
GTTTTAACTG CAGCTGAAAT GGAGGCGTTC AAGCAGAGAC ACTCATACCC TGAGAGATTG 
GTTCGCAGCA GAAGCTCTGA TATAGTGTCT TCTGTGCGGC GGCCCATGAG TGACCCCAGC 
TGGAACCGAC GTCCAGGGAA TGAAGAACTT CCTCCAGCTG CAGCCACTGG TGCTACTTCT 
TTGGTGGCTG CACCTCACTC GTCATCTTCG TCCCCGAGTA AGGACTCTTC TAGAGGAGAG 
ACTGAGGAAC GCAAGGACAG TGACGATGAG AGGTCAGACA GGAGCAGGCC TTGGTGGAGG 
AAGCGCTTTG TTTCAGCTAT GCCTAAAGAT GAGCCCAGCC CTAGGCTCAG TGCTCAAGCT 
CAGGTTGCTG AAGATATTCT GGACAAGTAC CGGAATGCCA TTAAACGGAC CAGCCCCAGT 
GAAGGAGCAA TGGCAAATGA TGAGAGTGCA GAGGTCATGG GTGATGGTGA AAGTGCCCAC 
GACTCTCCTC GTGAGGAAGC ACTGCAGAAC ATCTCAGCCG ACGACCTCCC GGACTCCGCC 
AGCCAGGCAG CCCACCCTCA GGACTCTGCT TTCTCTTACA GAGATGTAAA AAAGAAGCTG 
AGGCTCGCAC TTTGCTCTGC AGACTCTGTG GCCTTCCCAG TGCTAACTCA TTCAACAAGG 
AATGGATTGC CAGACCACAC AGACCCAGAA GACAATGAAA TTGTATGCTT CTTAAAAGTT 
CAAATAGCTG AAGCAATTAA TTTACAAGAT AAGAGCTTAA TGGCTCAACT TCAAGAAACA 
ATGCGTTGTG TGTGCCGCTT TGACAATAGG ACTTGTAGGA AGTTGCTGGC ATCCATTGCT 
GAAGACTACA GAAAAAGAGC TCCGTATATT GCTTATCTTA CCCGCTGCCG ACAAGGACTG 
CAGACCACAC AGGCTCACTT GGAAAGGCTC TTGCAAAGAG TTTTGCGGGA CAAAGAGGTG 
GCCAATCGGT ACTTCACCAC TGTCTGTGTG CGGTTACTGC TTGAGAGCAA AGAAAAGAAA 
ATCAGGGAGT TCATTCAAGA CTTTCAGAAA CTTACTGCTG CTGATGATAA AACGGCTCAG 
GTGGAGGATT TTCTACAGTT CCTGTATGGT GTGATGGCTC AGGATGTCAT ATGGCAGAAT 
GCCAGTGAGG AGCAGCTGCA GGATGCCCAG CTGGCCATCG AGCGGAGTGT GATGAATCGG 
ATCTTCAAGC TTGCTTTCTA CCCTAACCAG GATGGGGACA TACTTCGTGA CCAGGTTCTG 
CATGAACATA TCCAGAGATT ATCTAAAGTA GTGACTGCAA ACCACAGAGC TCTGCAGATC 
CCTGAGGTTT ATCTCCGTGA GGCACCATGG CCATCTGCAC AGTCAGAAAT CAGGACAATA 
AGTGCTTACA AAACGCCCCG CGACAAAGTG CAGTGCATCC TGAGGATGTG CTCCACCATC 
ATGAACCTCT TGAGCCTGGC CAACGAGGAC TCTGTCCCCG GGGCAGATGA CTTTGTTCCT 
GTGTTGGTGT TTGTGTTAAT AAAGGCAAAC CCACCCTGCC TGCTGTCCAC TGTGCAGTAC 
ATCAGCAGCT TCTATGCCAG CTGTCTGTCT GGAGAGGAGT CCTACTGGTG GATGCAGTTC 
ACAGCAGCCG TGGAGTTTAT TAAAACCATC GATGACAGAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAPVD1 ( XM_030251957.1 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_030107817.1 Mus musculus GTPase activating protein and VPS9 domains 1 (Gapvd1), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OMu87969
Clone ID Related Accession (Same CDS sequence)	XM_030251957.1
Accession Version	XM_030251957.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1986bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	GTPase-activating protein and VPS9 domain-containing protein 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGC TAGATATTCA CACATTGGCC CACCACCTCA AGCAGGAACG CTTATATGTG 
AGCTCTGAGA AACAGCTCAT TCAGAGGCTA AATGCCGATG TACTGAAGAC AGCTGAAAAG 
CTGTATCGAA CAGCGTGGAT TGCAAAGCAA CAGAGGATTA ATTTGGATCG TCTTATTATA 
ACCAGTGCTG AAGCTTCCCC TGCTGAGTGT TGCCAGCATG CCAAGATTTT GGAAGATACA 
CAGTTTGTTG ATGGATATAA GCAACTGGGA TTTCAGGAAA CAGCTTATGG AGAATTCCTG 
AGTCGGTTAA GAGAAAATCC ACGACTTATT GCCTCCTCTT TGGTTGCTGG AGAAAAACTC 
AATCAGGAGA ACACACAAAG TGTTATCTAC ACTGTTTTTA CCTCCTTGTA TGGCAACTGT 
ATTATGCAAG AAGATGAAAG CTACCTCCTT CAGGTTTTAC GATACTTGAT TGAATTTGAA 
CTTAAAGAAA GTGACAACCC CAGGCGCCTT TTGAGGAGAG GAACTTGTGC CTTCAGCATC 
TTATTTAAAC TTTTTTCTGA AGGACTGTTT TCTGCCAAAC TTTTCCTTAC GGCTACTTTG 
CATGAGCCAA TCATGCAGCT CCTCGTAGAG GATGAAGACC ACCTGGAAAC AGATCCAAAC 
AAGCTGATAG AGAGATTCTC TCCAGCTCAG CAAGAAAAAC TCTTTGGAGA GAAAGGCTCA 
GATAGATTTA GGCAAAAGGT TCAAGAAATG GTAGATTCCA ATGAGGCTAA ACTAGTGGCT 
TTGGTGAACA AATTTATTGG TTATCTTAAA CAGAATACAT ACTGCTTTCC ACATAGTTTG 
AGGTGGATTG TGTCACAGAT GTACAAAACT CTTTCTTGTG TAGACAGATT GGAGGTTGGG 
GAAGTCAGAG CAATGTGTAC CGATCTTCTG CTGGCTTGCT TCATTTGTCC TGCAGTTGTC 
AATCCAGAGC AGTATGGGAT AATATCTGAT GCTCCTATTA ATGAGGTGGC AAGATTTAAC 
CTGATGCAGG TTGGCCGCCT TTTGCAGCAG CTAGCAATGA CTGGCACTGA AGAAGGAGAT 
CCCCGGACCA AAAATAGCCT TGGAAAATTT GACAAAGGCT GTGTCGCTGC TTTCCTTGAT 
GTTGTAATTG GGGGCCGTGC AGTGGAGACT CCGCCAATGT CCTCTGTTAA TCTTCTGGAA 
GGCTTGAGCA GAACTGTGGT GTATATAAGT TACAGTCAGC TTATTACTCT GGTAAATTTT 
ATGAAGAGTG TGATGTCTGG AGATCAACTG AAAGAAGATA GAATGGCTCT TGACAATTTG 
TTGGCAAACC TGCCCCAGGC TAAGCCAGGG AAGAGCAGCA GTCTGGACAT GACTCCCTAT 
AGCACTCCTC AGATGTCTCC AGCAACCACT CCAGCCAACA AGAAGAATCG ATTACCTATA 
GGACAACAGT TAGCAGCCAT CACTGCCTGG GATTCGTCAG CCACCAATCT TACTGCTCAT 
ATTCCTCTAG TAACCCCATT TGCAACTCGG AGCAGAAGCC GAAGTAATAT GTTAATGGAC 
CTACATATGG ATCATGAAGG ATCATCTCAA GAAACCATCC AGGAGGTACA GCCAGAAGAG 
GTGCTGGTCA TTTCCTTAGG GACAGGCCCT CAGCTCACTC CAGGGATGAT GTCAGAAAAT 
GAGGTTCTAA ACATGCAGCT TTCGGATGGA GGACAAGGAG ATGTCCCTGT TGATGAAAAC 
AAACTCCACG GTAAACCTGA TAAAACCTTG CGCTTTTCCC TCTGCAGTGA TAATCTGGAA 
GGCATATCTG AAGGTCCTTC CAATCGCTCC AATTCGGTGT CTTCTCTCGA CCTGGAGGGA 
GAGTCTGTAT CTGAACTTGG GGCAGGACCT TCTGGGAGTA ACGGAGTTGA AGCACTGCAG 
CTGTTAGAGC ACGAGCAAGG TGTTCTGGGT GATTCCGGTG TAGGAGTGGA CAGCCTTCAC 
ATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030107817.1
CDS	328..2313
Translation	MVKLDIHTLAHHLKQERLYVSSEKQLIQRLNADVLKTAEKLYRTAWIAKQQRINLDRLIITSAEASPAECCQHAKILEDTQFVDGYKQLGFQETAYGEFLSRLRENPRLIASSLVAGEKLNQENTQSVIYTVFTSLYGNCIMQEDESYLLQVLRYLIEFELKESDNPRRLLRRGTCAFSILFKLFSEGLFSAKLFLTATLHEPIMQLLVEDEDHLETDPNKLIERFSPAQQEKLFGEKGSDRFRQKVQEMVDSNEAKLVALVNKFIGYLKQNTYCFPHSLRWIVSQMYKTLSCVDRLEVGEVRAMCTDLLLACFICPAVVNPEQYGIISDAPINEVARFNLMQVGRLLQQLAMTGTEEGDPRTKNSLGKFDKGCVAAFLDVVIGGRAVETPPMSSVNLLEGLSRTVVYISYSQLITLVNFMKSVMSGDQLKEDRMALDNLLANLPQAKPGKSSSLDMTPYSTPQMSPATTPANKKNRLPIGQQLAAITAWDSSATNLTAHIPLVTPFATRSRSRSNMLMDLHMDHEGSSQETIQEVQPEEVLVISLGTGPQLTPGMMSENEVLNMQLSDGGQGDVPVDENKLHGKPDKTLRFSLCSDNLEGISEGPSNRSNSVSSLDLEGESVSELGAGPSGSNGVEALQLLEHEQGVLGDSGVGVDSLHM

Target ORF information:

RefSeq Version	XM_030251957.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus GTPase activating protein and VPS9 domains 1 (Gapvd1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030251957.1

ORF Insert Sequence:

ATGGTGAAGC TAGATATTCA CACATTGGCC CACCACCTCA AGCAGGAACG CTTATATGTG 
AGCTCTGAGA AACAGCTCAT TCAGAGGCTA AATGCCGATG TACTGAAGAC AGCTGAAAAG 
CTGTATCGAA CAGCGTGGAT TGCAAAGCAA CAGAGGATTA ATTTGGATCG TCTTATTATA 
ACCAGTGCTG AAGCTTCCCC TGCTGAGTGT TGCCAGCATG CCAAGATTTT GGAAGATACA 
CAGTTTGTTG ATGGATATAA GCAACTGGGA TTTCAGGAAA CAGCTTATGG AGAATTCCTG 
AGTCGGTTAA GAGAAAATCC ACGACTTATT GCCTCCTCTT TGGTTGCTGG AGAAAAACTC 
AATCAGGAGA ACACACAAAG TGTTATCTAC ACTGTTTTTA CCTCCTTGTA TGGCAACTGT 
ATTATGCAAG AAGATGAAAG CTACCTCCTT CAGGTTTTAC GATACTTGAT TGAATTTGAA 
CTTAAAGAAA GTGACAACCC CAGGCGCCTT TTGAGGAGAG GAACTTGTGC CTTCAGCATC 
TTATTTAAAC TTTTTTCTGA AGGACTGTTT TCTGCCAAAC TTTTCCTTAC GGCTACTTTG 
CATGAGCCAA TCATGCAGCT CCTCGTAGAG GATGAAGACC ACCTGGAAAC AGATCCAAAC 
AAGCTGATAG AGAGATTCTC TCCAGCTCAG CAAGAAAAAC TCTTTGGAGA GAAAGGCTCA 
GATAGATTTA GGCAAAAGGT TCAAGAAATG GTAGATTCCA ATGAGGCTAA ACTAGTGGCT 
TTGGTGAACA AATTTATTGG TTATCTTAAA CAGAATACAT ACTGCTTTCC ACATAGTTTG 
AGGTGGATTG TGTCACAGAT GTACAAAACT CTTTCTTGTG TAGACAGATT GGAGGTTGGG 
GAAGTCAGAG CAATGTGTAC CGATCTTCTG CTGGCTTGCT TCATTTGTCC TGCAGTTGTC 
AATCCAGAGC AGTATGGGAT AATATCTGAT GCTCCTATTA ATGAGGTGGC AAGATTTAAC 
CTGATGCAGG TTGGCCGCCT TTTGCAGCAG CTAGCAATGA CTGGCACTGA AGAAGGAGAT 
CCCCGGACCA AAAATAGCCT TGGAAAATTT GACAAAGGCT GTGTCGCTGC TTTCCTTGAT 
GTTGTAATTG GGGGCCGTGC AGTGGAGACT CCGCCAATGT CCTCTGTTAA TCTTCTGGAA 
GGCTTGAGCA GAACTGTGGT GTATATAAGT TACAGTCAGC TTATTACTCT GGTAAATTTT 
ATGAAGAGTG TGATGTCTGG AGATCAACTG AAAGAAGATA GAATGGCTCT TGACAATTTG 
TTGGCAAACC TGCCCCAGGC TAAGCCAGGG AAGAGCAGCA GTCTGGACAT GACTCCCTAT 
AGCACTCCTC AGATGTCTCC AGCAACCACT CCAGCCAACA AGAAGAATCG ATTACCTATA 
GGACAACAGT TAGCAGCCAT CACTGCCTGG GATTCGTCAG CCACCAATCT TACTGCTCAT 
ATTCCTCTAG TAACCCCATT TGCAACTCGG AGCAGAAGCC GAAGTAATAT GTTAATGGAC 
CTACATATGG ATCATGAAGG ATCATCTCAA GAAACCATCC AGGAGGTACA GCCAGAAGAG 
GTGCTGGTCA TTTCCTTAGG GACAGGCCCT CAGCTCACTC CAGGGATGAT GTCAGAAAAT 
GAGGTTCTAA ACATGCAGCT TTCGGATGGA GGACAAGGAG ATGTCCCTGT TGATGAAAAC 
AAACTCCACG GTAAACCTGA TAAAACCTTG CGCTTTTCCC TCTGCAGTGA TAATCTGGAA 
GGCATATCTG AAGGTCCTTC CAATCGCTCC AATTCGGTGT CTTCTCTCGA CCTGGAGGGA 
GAGTCTGTAT CTGAACTTGG GGCAGGACCT TCTGGGAGTA ACGGAGTTGA AGCACTGCAG 
CTGTTAGAGC ACGAGCAAGG TGTTCTGGGT GATTCCGGTG TAGGAGTGGA CAGCCTTCAC 
ATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAPVD1 ( XM_030251958.1 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_030107818.1 Mus musculus GTPase activating protein and VPS9 domains 1 (Gapvd1), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OMu87970
Clone ID Related Accession (Same CDS sequence)	XM_030251958.1
Accession Version	XM_030251958.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	GTPase-activating protein and VPS9 domain-containing protein 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGC TAGATATTCA CACATTGGCC CACCACCTCA AGCAGGAACG CTTATATGTG 
AGCTCTGAGA AACAGCTCAT TCAGAGGCTA AATGCCGATG TACTGAAGAC AGCTGAAAAG 
CTGTATCGAA CAGCGTGGAT TGCAAAGCAA CAGAGGATTA ATTTGGATCG TCTTATTATA 
ACCAGTGCTG AAGCTTCCCC TGCTGAGTGT TGCCAGCATG CCAAGATTTT GGAAGATACA 
CAGTTTGTTG ATGGATATAA GCAACTGGGA TTTCAGGAAA CAGCTTATGG AGAATTCCTG 
AGTCGGTTAA GAGAAAATCC ACGACTTATT GCCTCCTCTT TGGTTGCTGG AGAAAAACTC 
AATCAGGAGA ACACACAAAG TGTTATCTAC ACTGTTTTTA CCTCCTTGTA TGGCAACTGT 
ATTATGCAAG AAGATGAAAG CTACCTCCTT CAGGTTTTAC GATACTTGAT TGAATTTGAA 
CTTAAAGAAA GTGACAACCC CAGGCGCCTT TTGAGGAGAG GAACTTGTGC CTTCAGCATC 
TTATTTAAAC TTTTTTCTGA AGGACTGTTT TCTGCCAAAC TTTTCCTTAC GGCTACTTTG 
CATGAGCCAA TCATGCAGCT CCTCGTAGAG GATGAAGACC ACCTGGAAAC AGATCCAAAC 
AAGCTGATAG AGAGATTCTC TCCAGCTCAG CAAGAAAAAC TCTTTGGAGA GAAAGGCTCA 
GATAGATTTA GGCAAAAGGT TCAAGAAATG GTAGATTCCA ATGAGGCTAA ACTAGTGGCT 
TTGGTGAACA AATTTATTGG TTATCTTAAA CAGAATACAT ACTGCTTTCC ACATAGTTTG 
AGGTGGATTG TGTCACAGAT GTACAAAACT CTTTCTTGTG TAGACAGATT GGAGGTTGGG 
GAAGTCAGAG CAATGTGTAC CGATCTTCTG CTGGCTTGCT TCATTTGTCC TGCAGTTGTC 
AATCCAGAGC AGTATGGGAT AATATCTGAT GCTCCTATTA ATGAGGTGGC AAGATTTAAC 
CTGATGCAGG TTGGCCGCCT TTTGCAGCAG CTAGCAATGA CTGGCACTGA AGAAGGAGAT 
CCCCGGACCA AAAATAGCCT TGGAAAATTT GACAAAGGCT GTGTCGCTGC TTTCCTTGAT 
GTTGTAATTG GGGGCCGTGC AGTGGAGACT CCGCCAATGT CCTCTGTTAA TCTTCTGGAA 
GGCTTGAGCA GAACTGTGGT GTATATAAGT TACAGTCAGC TTATTACTCT GGTAAATTTT 
ATGAAGAGTG TGATGTCTGG AGATCAACTG AAAGAAGATA GAATGGCTCT TGACAATTTG 
TTGGCAAACC TGCCCCAGGC TAAGCCAGGG AAGAGCAGCA GTCTGGACAT GACTCCCTAT 
AGCACTCCTC AGATGTCTCC AGCAACCACT CCAGCCAACA AGAAGAATCG ATTACCTATA 
GGACAACAGT TAGCAGCCAT CACTGCCTGG GATTCGTCAG CCACCAATCT TACTGCTCAT 
ATTCCTCTAG TAACCCCATT TGCAACTCGG AGCAGAAGCC GAAGTAATAT GTTAATGGAC 
CTACATATGG ATCATGAAGG ATCATCTCAA GAAACCATCC AGGAGGTACA GCCAGAAGAG 
GTGCTGGTCA TTTCCTTAGG GACAGGCCCT CAGCTCACTC CAGGGATGAT GTCAGAAAAT 
GAGGTTCTAA ACATGCAGCT TTCGGATGGA GGACAAGGAG ATGTCCCTGT TGATGAAAAC 
AAACTCCACG GTAAACCTGA TAAAACCTTG CGCTTTTCCC TCTGCAGTGA TAATCTGGAA 
GGCATATCTG AAGGTCCTTC CAATCGCTCC AATTCGGTGT CTTCTCTCGA CCTGGAGGGA 
GAGTCTGTAT CTGAACTTGG GGCAGGACCT TCTGGGAGTA ACGGAGTTGA AGCACTGCAG 
CTGTTAGAGC ACGAGCAAGA GAAACCTGAA ATTAGTATAC AGTCAAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030107818.1
CDS	328..2298
Translation	MVKLDIHTLAHHLKQERLYVSSEKQLIQRLNADVLKTAEKLYRTAWIAKQQRINLDRLIITSAEASPAECCQHAKILEDTQFVDGYKQLGFQETAYGEFLSRLRENPRLIASSLVAGEKLNQENTQSVIYTVFTSLYGNCIMQEDESYLLQVLRYLIEFELKESDNPRRLLRRGTCAFSILFKLFSEGLFSAKLFLTATLHEPIMQLLVEDEDHLETDPNKLIERFSPAQQEKLFGEKGSDRFRQKVQEMVDSNEAKLVALVNKFIGYLKQNTYCFPHSLRWIVSQMYKTLSCVDRLEVGEVRAMCTDLLLACFICPAVVNPEQYGIISDAPINEVARFNLMQVGRLLQQLAMTGTEEGDPRTKNSLGKFDKGCVAAFLDVVIGGRAVETPPMSSVNLLEGLSRTVVYISYSQLITLVNFMKSVMSGDQLKEDRMALDNLLANLPQAKPGKSSSLDMTPYSTPQMSPATTPANKKNRLPIGQQLAAITAWDSSATNLTAHIPLVTPFATRSRSRSNMLMDLHMDHEGSSQETIQEVQPEEVLVISLGTGPQLTPGMMSENEVLNMQLSDGGQGDVPVDENKLHGKPDKTLRFSLCSDNLEGISEGPSNRSNSVSSLDLEGESVSELGAGPSGSNGVEALQLLEHEQEKPEISIQSR

Target ORF information:

RefSeq Version	XM_030251958.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus GTPase activating protein and VPS9 domains 1 (Gapvd1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030251958.1

ORF Insert Sequence:

ATGGTGAAGC TAGATATTCA CACATTGGCC CACCACCTCA AGCAGGAACG CTTATATGTG 
AGCTCTGAGA AACAGCTCAT TCAGAGGCTA AATGCCGATG TACTGAAGAC AGCTGAAAAG 
CTGTATCGAA CAGCGTGGAT TGCAAAGCAA CAGAGGATTA ATTTGGATCG TCTTATTATA 
ACCAGTGCTG AAGCTTCCCC TGCTGAGTGT TGCCAGCATG CCAAGATTTT GGAAGATACA 
CAGTTTGTTG ATGGATATAA GCAACTGGGA TTTCAGGAAA CAGCTTATGG AGAATTCCTG 
AGTCGGTTAA GAGAAAATCC ACGACTTATT GCCTCCTCTT TGGTTGCTGG AGAAAAACTC 
AATCAGGAGA ACACACAAAG TGTTATCTAC ACTGTTTTTA CCTCCTTGTA TGGCAACTGT 
ATTATGCAAG AAGATGAAAG CTACCTCCTT CAGGTTTTAC GATACTTGAT TGAATTTGAA 
CTTAAAGAAA GTGACAACCC CAGGCGCCTT TTGAGGAGAG GAACTTGTGC CTTCAGCATC 
TTATTTAAAC TTTTTTCTGA AGGACTGTTT TCTGCCAAAC TTTTCCTTAC GGCTACTTTG 
CATGAGCCAA TCATGCAGCT CCTCGTAGAG GATGAAGACC ACCTGGAAAC AGATCCAAAC 
AAGCTGATAG AGAGATTCTC TCCAGCTCAG CAAGAAAAAC TCTTTGGAGA GAAAGGCTCA 
GATAGATTTA GGCAAAAGGT TCAAGAAATG GTAGATTCCA ATGAGGCTAA ACTAGTGGCT 
TTGGTGAACA AATTTATTGG TTATCTTAAA CAGAATACAT ACTGCTTTCC ACATAGTTTG 
AGGTGGATTG TGTCACAGAT GTACAAAACT CTTTCTTGTG TAGACAGATT GGAGGTTGGG 
GAAGTCAGAG CAATGTGTAC CGATCTTCTG CTGGCTTGCT TCATTTGTCC TGCAGTTGTC 
AATCCAGAGC AGTATGGGAT AATATCTGAT GCTCCTATTA ATGAGGTGGC AAGATTTAAC 
CTGATGCAGG TTGGCCGCCT TTTGCAGCAG CTAGCAATGA CTGGCACTGA AGAAGGAGAT 
CCCCGGACCA AAAATAGCCT TGGAAAATTT GACAAAGGCT GTGTCGCTGC TTTCCTTGAT 
GTTGTAATTG GGGGCCGTGC AGTGGAGACT CCGCCAATGT CCTCTGTTAA TCTTCTGGAA 
GGCTTGAGCA GAACTGTGGT GTATATAAGT TACAGTCAGC TTATTACTCT GGTAAATTTT 
ATGAAGAGTG TGATGTCTGG AGATCAACTG AAAGAAGATA GAATGGCTCT TGACAATTTG 
TTGGCAAACC TGCCCCAGGC TAAGCCAGGG AAGAGCAGCA GTCTGGACAT GACTCCCTAT 
AGCACTCCTC AGATGTCTCC AGCAACCACT CCAGCCAACA AGAAGAATCG ATTACCTATA 
GGACAACAGT TAGCAGCCAT CACTGCCTGG GATTCGTCAG CCACCAATCT TACTGCTCAT 
ATTCCTCTAG TAACCCCATT TGCAACTCGG AGCAGAAGCC GAAGTAATAT GTTAATGGAC 
CTACATATGG ATCATGAAGG ATCATCTCAA GAAACCATCC AGGAGGTACA GCCAGAAGAG 
GTGCTGGTCA TTTCCTTAGG GACAGGCCCT CAGCTCACTC CAGGGATGAT GTCAGAAAAT 
GAGGTTCTAA ACATGCAGCT TTCGGATGGA GGACAAGGAG ATGTCCCTGT TGATGAAAAC 
AAACTCCACG GTAAACCTGA TAAAACCTTG CGCTTTTCCC TCTGCAGTGA TAATCTGGAA 
GGCATATCTG AAGGTCCTTC CAATCGCTCC AATTCGGTGT CTTCTCTCGA CCTGGAGGGA 
GAGTCTGTAT CTGAACTTGG GGCAGGACCT TCTGGGAGTA ACGGAGTTGA AGCACTGCAG 
CTGTTAGAGC ACGAGCAAGA GAAACCTGAA ATTAGTATAC AGTCAAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAPVD1 ( NM_025709.3 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_079985.2 Mus musculus GTPase activating protein and VPS9 domains 1 (Gapvd1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu00913
Clone ID Related Accession (Same CDS sequence)	NM_025709.3 , NM_025709.2
Accession Version	NM_025709.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4314bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-25
Organism	Mus musculus(house mouse)
Product	GTPase-activating protein and VPS9 domain-containing protein 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC060123.1 and AL845262.3. On Oct 4, 2017 this sequence version replaced NM_025709.2. Transcript Variant: This variant (2) uses an alternate in-frame splice junction compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC060123.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGAAGC TAGATATTCA CACATTGGCC CACCACCTCA AGCAGGAACG CTTATATGTG 
AGCTCTGAGA AACAGCTCAT TCAGAGGCTA AATGCCGATG TACTGAAGAC AGCTGAAAAG 
CTGTATCGAA CAGCGTGGAT TGCAAAGCAA CAGAGGATTA ATTTGGATCG TCTTATTATA 
ACCAGTGCTG AAGCTTCCCC TGCTGAGTGT TGCCAGCATG CCAAGATTTT GGAAGATACA 
CAGTTTGTTG ATGGATATAA GCAACTGGGA TTTCAGGAAA CAGCTTATGG AGAATTCCTG 
AGTCGGTTAA GAGAAAATCC ACGACTTATT GCCTCCTCTT TGGTTGCTGG AGAAAAACTC 
AATCAGGAGA ACACACAAAG TGTTATCTAC ACTGTTTTTA CCTCCTTGTA TGGCAACTGT 
ATTATGCAAG AAGATGAAAG CTACCTCCTT CAGGTTTTAC GATACTTGAT TGAATTTGAA 
CTTAAAGAAA GTGACAACCC CAGGCGCCTT TTGAGGAGAG GAACTTGTGC CTTCAGCATC 
TTATTTAAAC TTTTTTCTGA AGGACTGTTT TCTGCCAAAC TTTTCCTTAC GGCTACTTTG 
CATGAGCCAA TCATGCAGCT CCTCGTAGAG GATGAAGACC ACCTGGAAAC AGATCCAAAC 
AAGCTGATAG AGAGATTCTC TCCAGCTCAG CAAGAAAAAC TCTTTGGAGA GAAAGGCTCA 
GATAGATTTA GGCAAAAGGT TCAAGAAATG GTAGATTCCA ATGAGGCTAA ACTAGTGGCT 
TTGGTGAACA AATTTATTGG TTATCTTAAA CAGAATACAT ACTGCTTTCC ACATAGTTTG 
AGGTGGATTG TGTCACAGAT GTACAAAACT CTTTCTTGTG TAGACAGATT GGAGGTTGGG 
GAAGTCAGAG CAATGTGTAC CGATCTTCTG CTGGCTTGCT TCATTTGTCC TGCAGTTGTC 
AATCCAGAGC AGTATGGGAT AATATCTGAT GCTCCTATTA ATGAGGTGGC AAGATTTAAC 
CTGATGCAGG TTGGCCGCCT TTTGCAGCAG CTAGCAATGA CTGGCACTGA AGAAGGAGAT 
CCCCGGACCA AAAATAGCCT TGGAAAATTT GACAAAGGCT GTGTCGCTGC TTTCCTTGAT 
GTTGTAATTG GGGGCCGTGC AGTGGAGACT CCGCCAATGT CCTCTGTTAA TCTTCTGGAA 
GGCTTGAGCA GAACTGTGGT GTATATAAGT TACAGTCAGC TTATTACTCT GGTAAATTTT 
ATGAAGAGTG TGATGTCTGG AGATCAACTG AAAGAAGATA GAATGGCTCT TGACAATTTG 
TTGGCAAACC TGCCCCAGGC TAAGCCAGGG AAGAGCAGCA GTCTGGACAT GACTCCCTAT 
AGCACTCCTC AGATGTCTCC AGCAACCACT CCAGCCAACA AGAAGAATCG ATTACCTATA 
GCAACTCGGA GCAGAAGCCG AAGTAATATG TTAATGGACC TACATATGGA TCATGAAGGA 
TCATCTCAAG AAACCATCCA GGAGGTACAG CCAGAAGAGG TGCTGGTCAT TTCCTTAGGG 
ACAGGCCCTC AGCTCACTCC AGGGATGATG TCAGAAAATG AGGTTCTAAA CATGCAGCTT 
TCGGATGGAG GACAAGGAGA TGTCCCTGTT GATGAAAACA AACTCCACGG TCCTTCCAAT 
CGCTCCAATT CGGTGTCTTC TCTCGACCTG GAGGGAGAGT CTGTATCTGA ACTTGGGGCA 
GGACCTTCTG GGAGTAACGG AGTTGAAGCA CTGCAGCTGT TAGAGCACGA GCAAGCTACA 
ACACAAGATA ATCTTGATGA TAAACTGAGG AAGTTTGAGA TTCGTGACAT GATGGGACTA 
ACAGATGACA GGGACATATC CGAGACTGTG AGTGAGACCT GGAGCACTGA TGTCCTGGGA 
AGTGACTTCG ACCCGAACGT TGATGAAGAT CGTTTGCAAG AGATTGCAGG TGCAGCAGCA 
GAGAACGTGT TAGGTAGTTT GCTGTGCCTT CCAGGTTCAG GGTCAGTTCT CCTTGACCCT 
TGCACTGGCT CTACCATATC AGAGACCACA AGTGAAGCTT GGAGTGTAGA GGTATTGCCA 
AGTGACTCAG AGGCTCCAGA CTTAAAGCAG GAAGAGCGCC TCCAAGAGCT GGAGAGCTGT 
TCTGGACTGG GCAGCACATC TGATGATACG GATGTCAGGG AGGTCAGTTC CCGCCCCAGC 
ACACCAGGCC TCAGTGTTGT GTCCGGTATA AGTGCTACAT CGGAGGATAT TCCCAATAAG 
ATCGAAGACC TGAGATCTGA GTGCAGCTCT GATTTTGGGG GCAAAGATTC TGTAACTAGT 
CCAGATATGG ATGACATAGC CCATGGCGCT CATCAGCTGA CCTCTCCTCC CTCTCAGTCA 
GAGTCTCTGC TTGCCATGTT TGATCCACTG TCCTCACATG AAGGGGCCTC TGCTGTGGTA 
AGGCCAAAGG TTCACTATGC CCGACCCTCG CATCCACCTC CGGATCCTCC AATCCTGGAA 
GGTGCAGTGG GAGGAAATGA GGCCAGGTTG CCAAACTTTG GCTCTCACGT TTTAACTGCA 
GCTGAAATGG AGGCGTTCAA GCAGAGACAC TCATACCCTG AGAGATTGGT TCGCAGCAGA 
AGCTCTGATA TAGTGTCTTC TGTGCGGCGG CCCATGAGTG ACCCCAGCTG GAACCGACGT 
CCAGGGAATG AAGAACTTCC TCCAGCTGCA GCCACTGGTG CTACTTCTTT GGTGGCTGCA 
CCTCACTCGT CATCTTCGTC CCCGAGTAAG GACTCTTCTA GAGGAGAGAC TGAGGAACGC 
AAGGACAGTG ACGATGAGAG GTCAGACAGG AGCAGGCCTT GGTGGAGGAA GCGCTTTGTT 
TCAGCTATGC CTAAAGCTCC TATACCATTT AGAAAGAAAG AAAAACAAGA AAAAGACAAA 
GATGATCTGG GTCCTGACAG ATTCTCAACA CTCACAGATG AGCCCAGCCC TAGGCTCAGT 
GCTCAAGCTC AGGTTGCTGA AGATATTCTG GACAAGTACC GGAATGCCAT TAAACGGACC 
AGCCCCAGTG AAGGAGCAAT GGCAAATGAT GAGAGTGCAG AGGTCATGGG TGATGGTGAA 
AGTGCCCACG ACTCTCCTCG TGAGGAAGCA CTGCAGAACA TCTCAGCCGA CGACCTCCCG 
GACTCCGCCA GCCAGGCAGC CCACCCTCAG GACTCTGCTT TCTCTTACAG AGATGTAAAA 
AAGAAGCTGA GGCTCGCACT TTGCTCTGCA GACTCTGTGG CCTTCCCAGT GCTAACTCAT 
TCAACAAGGA ATGGATTGCC AGACCACACA GACCCAGAAG ACAATGAAAT TGTATGCTTC 
TTAAAAGTTC AAATAGCTGA AGCAATTAAT TTACAAGATA AGAGCTTAAT GGCTCAACTT 
CAAGAAACAA TGCGTTGTGT GTGCCGCTTT GACAATAGGA CTTGTAGGAA GTTGCTGGCA 
TCCATTGCTG AAGACTACAG AAAAAGAGCT CCGTATATTG CTTATCTTAC CCGCTGCCGA 
CAAGGACTGC AGACCACACA GGCTCACTTG GAAAGGCTCT TGCAAAGAGT TTTGCGGGAC 
AAAGAGGTGG CCAATCGGTA CTTCACCACT GTCTGTGTGC GGTTACTGCT TGAGAGCAAA 
GAAAAGAAAA TCAGGGAGTT CATTCAAGAC TTTCAGAAAC TTACTGCTGC TGATGATAAA 
ACGGCTCAGG TGGAGGATTT TCTACAGTTC CTGTATGGTG TGATGGCTCA GGATGTCATA 
TGGCAGAATG CCAGTGAGGA GCAGCTGCAG GATGCCCAGC TGGCCATCGA GCGGAGTGTG 
ATGAATCGGA TCTTCAAGCT TGCTTTCTAC CCTAACCAGG ATGGGGACAT ACTTCGTGAC 
CAGGTTCTGC ATGAACATAT CCAGAGATTA TCTAAAGTAG TGACTGCAAA CCACAGAGCT 
CTGCAGATCC CTGAGGTTTA TCTCCGTGAG GCACCATGGC CATCTGCACA GTCAGAAATC 
AGGACAATAA GTGCTTACAA AACGCCCCGC GACAAAGTGC AGTGCATCCT GAGGATGTGC 
TCCACCATCA TGAACCTCTT GAGCCTGGCC AACGAGGACT CTGTCCCCGG GGCAGATGAC 
TTTGTTCCTG TGTTGGTGTT TGTGTTAATA AAGGCAAACC CACCCTGCCT GCTGTCCACT 
GTGCAGTACA TCAGCAGCTT CTATGCCAGC TGTCTGTCTG GAGAGGAGTC CTACTGGTGG 
ATGCAGTTCA CAGCAGCCGT GGAGTTTATT AAAACCATCG ATGACAGAAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_079985.2
CDS	583..4896
Translation	MVKLDIHTLAHHLKQERLYVSSEKQLIQRLNADVLKTAEKLYRTAWIAKQQRINLDRLIITSAEASPAECCQHAKILEDTQFVDGYKQLGFQETAYGEFLSRLRENPRLIASSLVAGEKLNQENTQSVIYTVFTSLYGNCIMQEDESYLLQVLRYLIEFELKESDNPRRLLRRGTCAFSILFKLFSEGLFSAKLFLTATLHEPIMQLLVEDEDHLETDPNKLIERFSPAQQEKLFGEKGSDRFRQKVQEMVDSNEAKLVALVNKFIGYLKQNTYCFPHSLRWIVSQMYKTLSCVDRLEVGEVRAMCTDLLLACFICPAVVNPEQYGIISDAPINEVARFNLMQVGRLLQQLAMTGTEEGDPRTKNSLGKFDKGCVAAFLDVVIGGRAVETPPMSSVNLLEGLSRTVVYISYSQLITLVNFMKSVMSGDQLKEDRMALDNLLANLPQAKPGKSSSLDMTPYSTPQMSPATTPANKKNRLPIATRSRSRSNMLMDLHMDHEGSSQETIQEVQPEEVLVISLGTGPQLTPGMMSENEVLNMQLSDGGQGDVPVDENKLHGPSNRSNSVSSLDLEGESVSELGAGPSGSNGVEALQLLEHEQATTQDNLDDKLRKFEIRDMMGLTDDRDISETVSETWSTDVLGSDFDPNVDEDRLQEIAGAAAENVLGSLLCLPGSGSVLLDPCTGSTISETTSEAWSVEVLPSDSEAPDLKQEERLQELESCSGLGSTSDDTDVREVSSRPSTPGLSVVSGISATSEDIPNKIEDLRSECSSDFGGKDSVTSPDMDDIAHGAHQLTSPPSQSESLLAMFDPLSSHEGASAVVRPKVHYARPSHPPPDPPILEGAVGGNEARLPNFGSHVLTAAEMEAFKQRHSYPERLVRSRSSDIVSSVRRPMSDPSWNRRPGNEELPPAAATGATSLVAAPHSSSSSPSKDSSRGETEERKDSDDERSDRSRPWWRKRFVSAMPKAPIPFRKKEKQEKDKDDLGPDRFSTLTDEPSPRLSAQAQVAEDILDKYRNAIKRTSPSEGAMANDESAEVMGDGESAHDSPREEALQNISADDLPDSASQAAHPQDSAFSYRDVKKKLRLALCSADSVAFPVLTHSTRNGLPDHTDPEDNEIVCFLKVQIAEAINLQDKSLMAQLQETMRCVCRFDNRTCRKLLASIAEDYRKRAPYIAYLTRCRQGLQTTQAHLERLLQRVLRDKEVANRYFTTVCVRLLLESKEKKIREFIQDFQKLTAADDKTAQVEDFLQFLYGVMAQDVIWQNASEEQLQDAQLAIERSVMNRIFKLAFYPNQDGDILRDQVLHEHIQRLSKVVTANHRALQIPEVYLREAPWPSAQSEIRTISAYKTPRDKVQCILRMCSTIMNLLSLANEDSVPGADDFVPVLVFVLIKANPPCLLSTVQYISSFYASCLSGEESYWWMQFTAAVEFIKTIDDRK

Target ORF information:

RefSeq Version	NM_025709.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus GTPase activating protein and VPS9 domains 1 (Gapvd1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025709.3

ORF Insert Sequence:

ATGGTGAAGC TAGATATTCA CACATTGGCC CACCACCTCA AGCAGGAACG CTTATATGTG 
AGCTCTGAGA AACAGCTCAT TCAGAGGCTA AATGCCGATG TACTGAAGAC AGCTGAAAAG 
CTGTATCGAA CAGCGTGGAT TGCAAAGCAA CAGAGGATTA ATTTGGATCG TCTTATTATA 
ACCAGTGCTG AAGCTTCCCC TGCTGAGTGT TGCCAGCATG CCAAGATTTT GGAAGATACA 
CAGTTTGTTG ATGGATATAA GCAACTGGGA TTTCAGGAAA CAGCTTATGG AGAATTCCTG 
AGTCGGTTAA GAGAAAATCC ACGACTTATT GCCTCCTCTT TGGTTGCTGG AGAAAAACTC 
AATCAGGAGA ACACACAAAG TGTTATCTAC ACTGTTTTTA CCTCCTTGTA TGGCAACTGT 
ATTATGCAAG AAGATGAAAG CTACCTCCTT CAGGTTTTAC GATACTTGAT TGAATTTGAA 
CTTAAAGAAA GTGACAACCC CAGGCGCCTT TTGAGGAGAG GAACTTGTGC CTTCAGCATC 
TTATTTAAAC TTTTTTCTGA AGGACTGTTT TCTGCCAAAC TTTTCCTTAC GGCTACTTTG 
CATGAGCCAA TCATGCAGCT CCTCGTAGAG GATGAAGACC ACCTGGAAAC AGATCCAAAC 
AAGCTGATAG AGAGATTCTC TCCAGCTCAG CAAGAAAAAC TCTTTGGAGA GAAAGGCTCA 
GATAGATTTA GGCAAAAGGT TCAAGAAATG GTAGATTCCA ATGAGGCTAA ACTAGTGGCT 
TTGGTGAACA AATTTATTGG TTATCTTAAA CAGAATACAT ACTGCTTTCC ACATAGTTTG 
AGGTGGATTG TGTCACAGAT GTACAAAACT CTTTCTTGTG TAGACAGATT GGAGGTTGGG 
GAAGTCAGAG CAATGTGTAC CGATCTTCTG CTGGCTTGCT TCATTTGTCC TGCAGTTGTC 
AATCCAGAGC AGTATGGGAT AATATCTGAT GCTCCTATTA ATGAGGTGGC AAGATTTAAC 
CTGATGCAGG TTGGCCGCCT TTTGCAGCAG CTAGCAATGA CTGGCACTGA AGAAGGAGAT 
CCCCGGACCA AAAATAGCCT TGGAAAATTT GACAAAGGCT GTGTCGCTGC TTTCCTTGAT 
GTTGTAATTG GGGGCCGTGC AGTGGAGACT CCGCCAATGT CCTCTGTTAA TCTTCTGGAA 
GGCTTGAGCA GAACTGTGGT GTATATAAGT TACAGTCAGC TTATTACTCT GGTAAATTTT 
ATGAAGAGTG TGATGTCTGG AGATCAACTG AAAGAAGATA GAATGGCTCT TGACAATTTG 
TTGGCAAACC TGCCCCAGGC TAAGCCAGGG AAGAGCAGCA GTCTGGACAT GACTCCCTAT 
AGCACTCCTC AGATGTCTCC AGCAACCACT CCAGCCAACA AGAAGAATCG ATTACCTATA 
GCAACTCGGA GCAGAAGCCG AAGTAATATG TTAATGGACC TACATATGGA TCATGAAGGA 
TCATCTCAAG AAACCATCCA GGAGGTACAG CCAGAAGAGG TGCTGGTCAT TTCCTTAGGG 
ACAGGCCCTC AGCTCACTCC AGGGATGATG TCAGAAAATG AGGTTCTAAA CATGCAGCTT 
TCGGATGGAG GACAAGGAGA TGTCCCTGTT GATGAAAACA AACTCCACGG TCCTTCCAAT 
CGCTCCAATT CGGTGTCTTC TCTCGACCTG GAGGGAGAGT CTGTATCTGA ACTTGGGGCA 
GGACCTTCTG GGAGTAACGG AGTTGAAGCA CTGCAGCTGT TAGAGCACGA GCAAGCTACA 
ACACAAGATA ATCTTGATGA TAAACTGAGG AAGTTTGAGA TTCGTGACAT GATGGGACTA 
ACAGATGACA GGGACATATC CGAGACTGTG AGTGAGACCT GGAGCACTGA TGTCCTGGGA 
AGTGACTTCG ACCCGAACGT TGATGAAGAT CGTTTGCAAG AGATTGCAGG TGCAGCAGCA 
GAGAACGTGT TAGGTAGTTT GCTGTGCCTT CCAGGTTCAG GGTCAGTTCT CCTTGACCCT 
TGCACTGGCT CTACCATATC AGAGACCACA AGTGAAGCTT GGAGTGTAGA GGTATTGCCA 
AGTGACTCAG AGGCTCCAGA CTTAAAGCAG GAAGAGCGCC TCCAAGAGCT GGAGAGCTGT 
TCTGGACTGG GCAGCACATC TGATGATACG GATGTCAGGG AGGTCAGTTC CCGCCCCAGC 
ACACCAGGCC TCAGTGTTGT GTCCGGTATA AGTGCTACAT CGGAGGATAT TCCCAATAAG 
ATCGAAGACC TGAGATCTGA GTGCAGCTCT GATTTTGGGG GCAAAGATTC TGTAACTAGT 
CCAGATATGG ATGACATAGC CCATGGCGCT CATCAGCTGA CCTCTCCTCC CTCTCAGTCA 
GAGTCTCTGC TTGCCATGTT TGATCCACTG TCCTCACATG AAGGGGCCTC TGCTGTGGTA 
AGGCCAAAGG TTCACTATGC CCGACCCTCG CATCCACCTC CGGATCCTCC AATCCTGGAA 
GGTGCAGTGG GAGGAAATGA GGCCAGGTTG CCAAACTTTG GCTCTCACGT TTTAACTGCA 
GCTGAAATGG AGGCGTTCAA GCAGAGACAC TCATACCCTG AGAGATTGGT TCGCAGCAGA 
AGCTCTGATA TAGTGTCTTC TGTGCGGCGG CCCATGAGTG ACCCCAGCTG GAACCGACGT 
CCAGGGAATG AAGAACTTCC TCCAGCTGCA GCCACTGGTG CTACTTCTTT GGTGGCTGCA 
CCTCACTCGT CATCTTCGTC CCCGAGTAAG GACTCTTCTA GAGGAGAGAC TGAGGAACGC 
AAGGACAGTG ACGATGAGAG GTCAGACAGG AGCAGGCCTT GGTGGAGGAA GCGCTTTGTT 
TCAGCTATGC CTAAAGCTCC TATACCATTT AGAAAGAAAG AAAAACAAGA AAAAGACAAA 
GATGATCTGG GTCCTGACAG ATTCTCAACA CTCACAGATG AGCCCAGCCC TAGGCTCAGT 
GCTCAAGCTC AGGTTGCTGA AGATATTCTG GACAAGTACC GGAATGCCAT TAAACGGACC 
AGCCCCAGTG AAGGAGCAAT GGCAAATGAT GAGAGTGCAG AGGTCATGGG TGATGGTGAA 
AGTGCCCACG ACTCTCCTCG TGAGGAAGCA CTGCAGAACA TCTCAGCCGA CGACCTCCCG 
GACTCCGCCA GCCAGGCAGC CCACCCTCAG GACTCTGCTT TCTCTTACAG AGATGTAAAA 
AAGAAGCTGA GGCTCGCACT TTGCTCTGCA GACTCTGTGG CCTTCCCAGT GCTAACTCAT 
TCAACAAGGA ATGGATTGCC AGACCACACA GACCCAGAAG ACAATGAAAT TGTATGCTTC 
TTAAAAGTTC AAATAGCTGA AGCAATTAAT TTACAAGATA AGAGCTTAAT GGCTCAACTT 
CAAGAAACAA TGCGTTGTGT GTGCCGCTTT GACAATAGGA CTTGTAGGAA GTTGCTGGCA 
TCCATTGCTG AAGACTACAG AAAAAGAGCT CCGTATATTG CTTATCTTAC CCGCTGCCGA 
CAAGGACTGC AGACCACACA GGCTCACTTG GAAAGGCTCT TGCAAAGAGT TTTGCGGGAC 
AAAGAGGTGG CCAATCGGTA CTTCACCACT GTCTGTGTGC GGTTACTGCT TGAGAGCAAA 
GAAAAGAAAA TCAGGGAGTT CATTCAAGAC TTTCAGAAAC TTACTGCTGC TGATGATAAA 
ACGGCTCAGG TGGAGGATTT TCTACAGTTC CTGTATGGTG TGATGGCTCA GGATGTCATA 
TGGCAGAATG CCAGTGAGGA GCAGCTGCAG GATGCCCAGC TGGCCATCGA GCGGAGTGTG 
ATGAATCGGA TCTTCAAGCT TGCTTTCTAC CCTAACCAGG ATGGGGACAT ACTTCGTGAC 
CAGGTTCTGC ATGAACATAT CCAGAGATTA TCTAAAGTAG TGACTGCAAA CCACAGAGCT 
CTGCAGATCC CTGAGGTTTA TCTCCGTGAG GCACCATGGC CATCTGCACA GTCAGAAATC 
AGGACAATAA GTGCTTACAA AACGCCCCGC GACAAAGTGC AGTGCATCCT GAGGATGTGC 
TCCACCATCA TGAACCTCTT GAGCCTGGCC AACGAGGACT CTGTCCCCGG GGCAGATGAC 
TTTGTTCCTG TGTTGGTGTT TGTGTTAATA AAGGCAAACC CACCCTGCCT GCTGTCCACT 
GTGCAGTACA TCAGCAGCTT CTATGCCAGC TGTCTGTCTG GAGAGGAGTC CTACTGGTGG 
ATGCAGTTCA CAGCAGCCGT GGAGTTTATT AAAACCATCG ATGACAGAAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAPVD1 ( NM_025709.2 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_079985.2 Mus musculus GTPase activating protein and VPS9 domains 1 (Gapvd1), mRNA.

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CloneID	OMu00913
Clone ID Related Accession (Same CDS sequence)	NM_025709.3 , NM_025709.2
Accession Version	NM_025709.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4314bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-14
Organism	Mus musculus(House mouse)
Product	GTPase-activating protein and VPS9 domain-containing protein 1
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC060123.1. On Dec 20, 2003 this sequence version replaced NM_025709.1. ##Evidence-Data-START## Transcript exon combination :: BC060123.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGTGAAGC TAGATATTCA CACATTGGCC CACCACCTCA AGCAGGAACG CTTATATGTG 
AGCTCTGAGA AACAGCTCAT TCAGAGGCTA AATGCCGATG TACTGAAGAC AGCTGAAAAG 
CTGTATCGAA CAGCGTGGAT TGCAAAGCAA CAGAGGATTA ATTTGGATCG TCTTATTATA 
ACCAGTGCTG AAGCTTCCCC TGCTGAGTGT TGCCAGCATG CCAAGATTTT GGAAGATACA 
CAGTTTGTTG ATGGATATAA GCAACTGGGA TTTCAGGAAA CAGCTTATGG AGAATTCCTG 
AGTCGGTTAA GAGAAAATCC ACGACTTATT GCCTCCTCTT TGGTTGCTGG AGAAAAACTC 
AATCAGGAGA ACACACAAAG TGTTATCTAC ACTGTTTTTA CCTCCTTGTA TGGCAACTGT 
ATTATGCAAG AAGATGAAAG CTACCTCCTT CAGGTTTTAC GATACTTGAT TGAATTTGAA 
CTTAAAGAAA GTGACAACCC CAGGCGCCTT TTGAGGAGAG GAACTTGTGC CTTCAGCATC 
TTATTTAAAC TTTTTTCTGA AGGACTGTTT TCTGCCAAAC TTTTCCTTAC GGCTACTTTG 
CATGAGCCAA TCATGCAGCT CCTCGTAGAG GATGAAGACC ACCTGGAAAC AGATCCAAAC 
AAGCTGATAG AGAGATTCTC TCCAGCTCAG CAAGAAAAAC TCTTTGGAGA GAAAGGCTCA 
GATAGATTTA GGCAAAAGGT TCAAGAAATG GTAGATTCCA ATGAGGCTAA ACTAGTGGCT 
TTGGTGAACA AATTTATTGG TTATCTTAAA CAGAATACAT ACTGCTTTCC ACATAGTTTG 
AGGTGGATTG TGTCACAGAT GTACAAAACT CTTTCTTGTG TAGACAGATT GGAGGTTGGG 
GAAGTCAGAG CAATGTGTAC CGATCTTCTG CTGGCTTGCT TCATTTGTCC TGCAGTTGTC 
AATCCAGAGC AGTATGGGAT AATATCTGAT GCTCCTATTA ATGAGGTGGC AAGATTTAAC 
CTGATGCAGG TTGGCCGCCT TTTGCAGCAG CTAGCAATGA CTGGCACTGA AGAAGGAGAT 
CCCCGGACCA AAAATAGCCT TGGAAAATTT GACAAAGGCT GTGTCGCTGC TTTCCTTGAT 
GTTGTAATTG GGGGCCGTGC AGTGGAGACT CCGCCAATGT CCTCTGTTAA TCTTCTGGAA 
GGCTTGAGCA GAACTGTGGT GTATATAAGT TACAGTCAGC TTATTACTCT GGTAAATTTT 
ATGAAGAGTG TGATGTCTGG AGATCAACTG AAAGAAGATA GAATGGCTCT TGACAATTTG 
TTGGCAAACC TGCCCCAGGC TAAGCCAGGG AAGAGCAGCA GTCTGGACAT GACTCCCTAT 
AGCACTCCTC AGATGTCTCC AGCAACCACT CCAGCCAACA AGAAGAATCG ATTACCTATA 
GCAACTCGGA GCAGAAGCCG AAGTAATATG TTAATGGACC TACATATGGA TCATGAAGGA 
TCATCTCAAG AAACCATCCA GGAGGTACAG CCAGAAGAGG TGCTGGTCAT TTCCTTAGGG 
ACAGGCCCTC AGCTCACTCC AGGGATGATG TCAGAAAATG AGGTTCTAAA CATGCAGCTT 
TCGGATGGAG GACAAGGAGA TGTCCCTGTT GATGAAAACA AACTCCACGG TCCTTCCAAT 
CGCTCCAATT CGGTGTCTTC TCTCGACCTG GAGGGAGAGT CTGTATCTGA ACTTGGGGCA 
GGACCTTCTG GGAGTAACGG AGTTGAAGCA CTGCAGCTGT TAGAGCACGA GCAAGCTACA 
ACACAAGATA ATCTTGATGA TAAACTGAGG AAGTTTGAGA TTCGTGACAT GATGGGACTA 
ACAGATGACA GGGACATATC CGAGACTGTG AGTGAGACCT GGAGCACTGA TGTCCTGGGA 
AGTGACTTCG ACCCGAACGT TGATGAAGAT CGTTTGCAAG AGATTGCAGG TGCAGCAGCA 
GAGAACGTGT TAGGTAGTTT GCTGTGCCTT CCAGGTTCAG GGTCAGTTCT CCTTGACCCT 
TGCACTGGCT CTACCATATC AGAGACCACA AGTGAAGCTT GGAGTGTAGA GGTATTGCCA 
AGTGACTCAG AGGCTCCAGA CTTAAAGCAG GAAGAGCGCC TCCAAGAGCT GGAGAGCTGT 
TCTGGACTGG GCAGCACATC TGATGATACG GATGTCAGGG AGGTCAGTTC CCGCCCCAGC 
ACACCAGGCC TCAGTGTTGT GTCCGGTATA AGTGCTACAT CGGAGGATAT TCCCAATAAG 
ATCGAAGACC TGAGATCTGA GTGCAGCTCT GATTTTGGGG GCAAAGATTC TGTAACTAGT 
CCAGATATGG ATGACATAGC CCATGGCGCT CATCAGCTGA CCTCTCCTCC CTCTCAGTCA 
GAGTCTCTGC TTGCCATGTT TGATCCACTG TCCTCACATG AAGGGGCCTC TGCTGTGGTA 
AGGCCAAAGG TTCACTATGC CCGACCCTCG CATCCACCTC CGGATCCTCC AATCCTGGAA 
GGTGCAGTGG GAGGAAATGA GGCCAGGTTG CCAAACTTTG GCTCTCACGT TTTAACTGCA 
GCTGAAATGG AGGCGTTCAA GCAGAGACAC TCATACCCTG AGAGATTGGT TCGCAGCAGA 
AGCTCTGATA TAGTGTCTTC TGTGCGGCGG CCCATGAGTG ACCCCAGCTG GAACCGACGT 
CCAGGGAATG AAGAACTTCC TCCAGCTGCA GCCACTGGTG CTACTTCTTT GGTGGCTGCA 
CCTCACTCGT CATCTTCGTC CCCGAGTAAG GACTCTTCTA GAGGAGAGAC TGAGGAACGC 
AAGGACAGTG ACGATGAGAG GTCAGACAGG AGCAGGCCTT GGTGGAGGAA GCGCTTTGTT 
TCAGCTATGC CTAAAGCTCC TATACCATTT AGAAAGAAAG AAAAACAAGA AAAAGACAAA 
GATGATCTGG GTCCTGACAG ATTCTCAACA CTCACAGATG AGCCCAGCCC TAGGCTCAGT 
GCTCAAGCTC AGGTTGCTGA AGATATTCTG GACAAGTACC GGAATGCCAT TAAACGGACC 
AGCCCCAGTG AAGGAGCAAT GGCAAATGAT GAGAGTGCAG AGGTCATGGG TGATGGTGAA 
AGTGCCCACG ACTCTCCTCG TGAGGAAGCA CTGCAGAACA TCTCAGCCGA CGACCTCCCG 
GACTCCGCCA GCCAGGCAGC CCACCCTCAG GACTCTGCTT TCTCTTACAG AGATGTAAAA 
AAGAAGCTGA GGCTCGCACT TTGCTCTGCA GACTCTGTGG CCTTCCCAGT GCTAACTCAT 
TCAACAAGGA ATGGATTGCC AGACCACACA GACCCAGAAG ACAATGAAAT TGTATGCTTC 
TTAAAAGTTC AAATAGCTGA AGCAATTAAT TTACAAGATA AGAGCTTAAT GGCTCAACTT 
CAAGAAACAA TGCGTTGTGT GTGCCGCTTT GACAATAGGA CTTGTAGGAA GTTGCTGGCA 
TCCATTGCTG AAGACTACAG AAAAAGAGCT CCGTATATTG CTTATCTTAC CCGCTGCCGA 
CAAGGACTGC AGACCACACA GGCTCACTTG GAAAGGCTCT TGCAAAGAGT TTTGCGGGAC 
AAAGAGGTGG CCAATCGGTA CTTCACCACT GTCTGTGTGC GGTTACTGCT TGAGAGCAAA 
GAAAAGAAAA TCAGGGAGTT CATTCAAGAC TTTCAGAAAC TTACTGCTGC TGATGATAAA 
ACGGCTCAGG TGGAGGATTT TCTACAGTTC CTGTATGGTG TGATGGCTCA GGATGTCATA 
TGGCAGAATG CCAGTGAGGA GCAGCTGCAG GATGCCCAGC TGGCCATCGA GCGGAGTGTG 
ATGAATCGGA TCTTCAAGCT TGCTTTCTAC CCTAACCAGG ATGGGGACAT ACTTCGTGAC 
CAGGTTCTGC ATGAACATAT CCAGAGATTA TCTAAAGTAG TGACTGCAAA CCACAGAGCT 
CTGCAGATCC CTGAGGTTTA TCTCCGTGAG GCACCATGGC CATCTGCACA GTCAGAAATC 
AGGACAATAA GTGCTTACAA AACGCCCCGC GACAAAGTGC AGTGCATCCT GAGGATGTGC 
TCCACCATCA TGAACCTCTT GAGCCTGGCC AACGAGGACT CTGTCCCCGG GGCAGATGAC 
TTTGTTCCTG TGTTGGTGTT TGTGTTAATA AAGGCAAACC CACCCTGCCT GCTGTCCACT 
GTGCAGTACA TCAGCAGCTT CTATGCCAGC TGTCTGTCTG GAGAGGAGTC CTACTGGTGG 
ATGCAGTTCA CAGCAGCCGT GGAGTTTATT AAAACCATCG ATGACAGAAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_079985.2
CDS	583..4896
Translation	MVKLDIHTLAHHLKQERLYVSSEKQLIQRLNADVLKTAEKLYRTAWIAKQQRINLDRLIITSAEASPAECCQHAKILEDTQFVDGYKQLGFQETAYGEFLSRLRENPRLIASSLVAGEKLNQENTQSVIYTVFTSLYGNCIMQEDESYLLQVLRYLIEFELKESDNPRRLLRRGTCAFSILFKLFSEGLFSAKLFLTATLHEPIMQLLVEDEDHLETDPNKLIERFSPAQQEKLFGEKGSDRFRQKVQEMVDSNEAKLVALVNKFIGYLKQNTYCFPHSLRWIVSQMYKTLSCVDRLEVGEVRAMCTDLLLACFICPAVVNPEQYGIISDAPINEVARFNLMQVGRLLQQLAMTGTEEGDPRTKNSLGKFDKGCVAAFLDVVIGGRAVETPPMSSVNLLEGLSRTVVYISYSQLITLVNFMKSVMSGDQLKEDRMALDNLLANLPQAKPGKSSSLDMTPYSTPQMSPATTPANKKNRLPIATRSRSRSNMLMDLHMDHEGSSQETIQEVQPEEVLVISLGTGPQLTPGMMSENEVLNMQLSDGGQGDVPVDENKLHGPSNRSNSVSSLDLEGESVSELGAGPSGSNGVEALQLLEHEQATTQDNLDDKLRKFEIRDMMGLTDDRDISETVSETWSTDVLGSDFDPNVDEDRLQEIAGAAAENVLGSLLCLPGSGSVLLDPCTGSTISETTSEAWSVEVLPSDSEAPDLKQEERLQELESCSGLGSTSDDTDVREVSSRPSTPGLSVVSGISATSEDIPNKIEDLRSECSSDFGGKDSVTSPDMDDIAHGAHQLTSPPSQSESLLAMFDPLSSHEGASAVVRPKVHYARPSHPPPDPPILEGAVGGNEARLPNFGSHVLTAAEMEAFKQRHSYPERLVRSRSSDIVSSVRRPMSDPSWNRRPGNEELPPAAATGATSLVAAPHSSSSSPSKDSSRGETEERKDSDDERSDRSRPWWRKRFVSAMPKAPIPFRKKEKQEKDKDDLGPDRFSTLTDEPSPRLSAQAQVAEDILDKYRNAIKRTSPSEGAMANDESAEVMGDGESAHDSPREEALQNISADDLPDSASQAAHPQDSAFSYRDVKKKLRLALCSADSVAFPVLTHSTRNGLPDHTDPEDNEIVCFLKVQIAEAINLQDKSLMAQLQETMRCVCRFDNRTCRKLLASIAEDYRKRAPYIAYLTRCRQGLQTTQAHLERLLQRVLRDKEVANRYFTTVCVRLLLESKEKKIREFIQDFQKLTAADDKTAQVEDFLQFLYGVMAQDVIWQNASEEQLQDAQLAIERSVMNRIFKLAFYPNQDGDILRDQVLHEHIQRLSKVVTANHRALQIPEVYLREAPWPSAQSEIRTISAYKTPRDKVQCILRMCSTIMNLLSLANEDSVPGADDFVPVLVFVLIKANPPCLLSTVQYISSFYASCLSGEESYWWMQFTAAVEFIKTIDDRK

Target ORF information:

RefSeq Version	NM_025709.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus GTPase activating protein and VPS9 domains 1 (Gapvd1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025709.2

ORF Insert Sequence:

ATGGTGAAGC TAGATATTCA CACATTGGCC CACCACCTCA AGCAGGAACG CTTATATGTG 
AGCTCTGAGA AACAGCTCAT TCAGAGGCTA AATGCCGATG TACTGAAGAC AGCTGAAAAG 
CTGTATCGAA CAGCGTGGAT TGCAAAGCAA CAGAGGATTA ATTTGGATCG TCTTATTATA 
ACCAGTGCTG AAGCTTCCCC TGCTGAGTGT TGCCAGCATG CCAAGATTTT GGAAGATACA 
CAGTTTGTTG ATGGATATAA GCAACTGGGA TTTCAGGAAA CAGCTTATGG AGAATTCCTG 
AGTCGGTTAA GAGAAAATCC ACGACTTATT GCCTCCTCTT TGGTTGCTGG AGAAAAACTC 
AATCAGGAGA ACACACAAAG TGTTATCTAC ACTGTTTTTA CCTCCTTGTA TGGCAACTGT 
ATTATGCAAG AAGATGAAAG CTACCTCCTT CAGGTTTTAC GATACTTGAT TGAATTTGAA 
CTTAAAGAAA GTGACAACCC CAGGCGCCTT TTGAGGAGAG GAACTTGTGC CTTCAGCATC 
TTATTTAAAC TTTTTTCTGA AGGACTGTTT TCTGCCAAAC TTTTCCTTAC GGCTACTTTG 
CATGAGCCAA TCATGCAGCT CCTCGTAGAG GATGAAGACC ACCTGGAAAC AGATCCAAAC 
AAGCTGATAG AGAGATTCTC TCCAGCTCAG CAAGAAAAAC TCTTTGGAGA GAAAGGCTCA 
GATAGATTTA GGCAAAAGGT TCAAGAAATG GTAGATTCCA ATGAGGCTAA ACTAGTGGCT 
TTGGTGAACA AATTTATTGG TTATCTTAAA CAGAATACAT ACTGCTTTCC ACATAGTTTG 
AGGTGGATTG TGTCACAGAT GTACAAAACT CTTTCTTGTG TAGACAGATT GGAGGTTGGG 
GAAGTCAGAG CAATGTGTAC CGATCTTCTG CTGGCTTGCT TCATTTGTCC TGCAGTTGTC 
AATCCAGAGC AGTATGGGAT AATATCTGAT GCTCCTATTA ATGAGGTGGC AAGATTTAAC 
CTGATGCAGG TTGGCCGCCT TTTGCAGCAG CTAGCAATGA CTGGCACTGA AGAAGGAGAT 
CCCCGGACCA AAAATAGCCT TGGAAAATTT GACAAAGGCT GTGTCGCTGC TTTCCTTGAT 
GTTGTAATTG GGGGCCGTGC AGTGGAGACT CCGCCAATGT CCTCTGTTAA TCTTCTGGAA 
GGCTTGAGCA GAACTGTGGT GTATATAAGT TACAGTCAGC TTATTACTCT GGTAAATTTT 
ATGAAGAGTG TGATGTCTGG AGATCAACTG AAAGAAGATA GAATGGCTCT TGACAATTTG 
TTGGCAAACC TGCCCCAGGC TAAGCCAGGG AAGAGCAGCA GTCTGGACAT GACTCCCTAT 
AGCACTCCTC AGATGTCTCC AGCAACCACT CCAGCCAACA AGAAGAATCG ATTACCTATA 
GCAACTCGGA GCAGAAGCCG AAGTAATATG TTAATGGACC TACATATGGA TCATGAAGGA 
TCATCTCAAG AAACCATCCA GGAGGTACAG CCAGAAGAGG TGCTGGTCAT TTCCTTAGGG 
ACAGGCCCTC AGCTCACTCC AGGGATGATG TCAGAAAATG AGGTTCTAAA CATGCAGCTT 
TCGGATGGAG GACAAGGAGA TGTCCCTGTT GATGAAAACA AACTCCACGG TCCTTCCAAT 
CGCTCCAATT CGGTGTCTTC TCTCGACCTG GAGGGAGAGT CTGTATCTGA ACTTGGGGCA 
GGACCTTCTG GGAGTAACGG AGTTGAAGCA CTGCAGCTGT TAGAGCACGA GCAAGCTACA 
ACACAAGATA ATCTTGATGA TAAACTGAGG AAGTTTGAGA TTCGTGACAT GATGGGACTA 
ACAGATGACA GGGACATATC CGAGACTGTG AGTGAGACCT GGAGCACTGA TGTCCTGGGA 
AGTGACTTCG ACCCGAACGT TGATGAAGAT CGTTTGCAAG AGATTGCAGG TGCAGCAGCA 
GAGAACGTGT TAGGTAGTTT GCTGTGCCTT CCAGGTTCAG GGTCAGTTCT CCTTGACCCT 
TGCACTGGCT CTACCATATC AGAGACCACA AGTGAAGCTT GGAGTGTAGA GGTATTGCCA 
AGTGACTCAG AGGCTCCAGA CTTAAAGCAG GAAGAGCGCC TCCAAGAGCT GGAGAGCTGT 
TCTGGACTGG GCAGCACATC TGATGATACG GATGTCAGGG AGGTCAGTTC CCGCCCCAGC 
ACACCAGGCC TCAGTGTTGT GTCCGGTATA AGTGCTACAT CGGAGGATAT TCCCAATAAG 
ATCGAAGACC TGAGATCTGA GTGCAGCTCT GATTTTGGGG GCAAAGATTC TGTAACTAGT 
CCAGATATGG ATGACATAGC CCATGGCGCT CATCAGCTGA CCTCTCCTCC CTCTCAGTCA 
GAGTCTCTGC TTGCCATGTT TGATCCACTG TCCTCACATG AAGGGGCCTC TGCTGTGGTA 
AGGCCAAAGG TTCACTATGC CCGACCCTCG CATCCACCTC CGGATCCTCC AATCCTGGAA 
GGTGCAGTGG GAGGAAATGA GGCCAGGTTG CCAAACTTTG GCTCTCACGT TTTAACTGCA 
GCTGAAATGG AGGCGTTCAA GCAGAGACAC TCATACCCTG AGAGATTGGT TCGCAGCAGA 
AGCTCTGATA TAGTGTCTTC TGTGCGGCGG CCCATGAGTG ACCCCAGCTG GAACCGACGT 
CCAGGGAATG AAGAACTTCC TCCAGCTGCA GCCACTGGTG CTACTTCTTT GGTGGCTGCA 
CCTCACTCGT CATCTTCGTC CCCGAGTAAG GACTCTTCTA GAGGAGAGAC TGAGGAACGC 
AAGGACAGTG ACGATGAGAG GTCAGACAGG AGCAGGCCTT GGTGGAGGAA GCGCTTTGTT 
TCAGCTATGC CTAAAGCTCC TATACCATTT AGAAAGAAAG AAAAACAAGA AAAAGACAAA 
GATGATCTGG GTCCTGACAG ATTCTCAACA CTCACAGATG AGCCCAGCCC TAGGCTCAGT 
GCTCAAGCTC AGGTTGCTGA AGATATTCTG GACAAGTACC GGAATGCCAT TAAACGGACC 
AGCCCCAGTG AAGGAGCAAT GGCAAATGAT GAGAGTGCAG AGGTCATGGG TGATGGTGAA 
AGTGCCCACG ACTCTCCTCG TGAGGAAGCA CTGCAGAACA TCTCAGCCGA CGACCTCCCG 
GACTCCGCCA GCCAGGCAGC CCACCCTCAG GACTCTGCTT TCTCTTACAG AGATGTAAAA 
AAGAAGCTGA GGCTCGCACT TTGCTCTGCA GACTCTGTGG CCTTCCCAGT GCTAACTCAT 
TCAACAAGGA ATGGATTGCC AGACCACACA GACCCAGAAG ACAATGAAAT TGTATGCTTC 
TTAAAAGTTC AAATAGCTGA AGCAATTAAT TTACAAGATA AGAGCTTAAT GGCTCAACTT 
CAAGAAACAA TGCGTTGTGT GTGCCGCTTT GACAATAGGA CTTGTAGGAA GTTGCTGGCA 
TCCATTGCTG AAGACTACAG AAAAAGAGCT CCGTATATTG CTTATCTTAC CCGCTGCCGA 
CAAGGACTGC AGACCACACA GGCTCACTTG GAAAGGCTCT TGCAAAGAGT TTTGCGGGAC 
AAAGAGGTGG CCAATCGGTA CTTCACCACT GTCTGTGTGC GGTTACTGCT TGAGAGCAAA 
GAAAAGAAAA TCAGGGAGTT CATTCAAGAC TTTCAGAAAC TTACTGCTGC TGATGATAAA 
ACGGCTCAGG TGGAGGATTT TCTACAGTTC CTGTATGGTG TGATGGCTCA GGATGTCATA 
TGGCAGAATG CCAGTGAGGA GCAGCTGCAG GATGCCCAGC TGGCCATCGA GCGGAGTGTG 
ATGAATCGGA TCTTCAAGCT TGCTTTCTAC CCTAACCAGG ATGGGGACAT ACTTCGTGAC 
CAGGTTCTGC ATGAACATAT CCAGAGATTA TCTAAAGTAG TGACTGCAAA CCACAGAGCT 
CTGCAGATCC CTGAGGTTTA TCTCCGTGAG GCACCATGGC CATCTGCACA GTCAGAAATC 
AGGACAATAA GTGCTTACAA AACGCCCCGC GACAAAGTGC AGTGCATCCT GAGGATGTGC 
TCCACCATCA TGAACCTCTT GAGCCTGGCC AACGAGGACT CTGTCCCCGG GGCAGATGAC 
TTTGTTCCTG TGTTGGTGTT TGTGTTAATA AAGGCAAACC CACCCTGCCT GCTGTCCACT 
GTGCAGTACA TCAGCAGCTT CTATGCCAGC TGTCTGTCTG GAGAGGAGTC CTACTGGTGG 
ATGCAGTTCA CAGCAGCCGT GGAGTTTATT AAAACCATCG ATGACAGAAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAPVD1 ( XM_017319195.2 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_017174684.1 Mus musculus GTPase activating protein and VPS9 domains 1 (Gapvd1), transcript variant X1, mRNA.

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CloneID	OMu69988
Clone ID Related Accession (Same CDS sequence)	XM_017319195.2 , XM_017319196.2 , XM_011239145.1 , XM_017319195.1 , XM_017319196.1
Accession Version	XM_017319195.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4458bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	GTPase-activating protein and VPS9 domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017319195.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGC TAGATATTCA CACATTGGCC CACCACCTCA AGCAGGAACG CTTATATGTG 
AGCTCTGAGA AACAGCTCAT TCAGAGGCTA AATGCCGATG TACTGAAGAC AGCTGAAAAG 
CTGTATCGAA CAGCGTGGAT TGCAAAGCAA CAGAGGATTA ATTTGGATCG TCTTATTATA 
ACCAGTGCTG AAGCTTCCCC TGCTGAGTGT TGCCAGCATG CCAAGATTTT GGAAGATACA 
CAGTTTGTTG ATGGATATAA GCAACTGGGA TTTCAGGAAA CAGCTTATGG AGAATTCCTG 
AGTCGGTTAA GAGAAAATCC ACGACTTATT GCCTCCTCTT TGGTTGCTGG AGAAAAACTC 
AATCAGGAGA ACACACAAAG TGTTATCTAC ACTGTTTTTA CCTCCTTGTA TGGCAACTGT 
ATTATGCAAG AAGATGAAAG CTACCTCCTT CAGGTTTTAC GATACTTGAT TGAATTTGAA 
CTTAAAGAAA GTGACAACCC CAGGCGCCTT TTGAGGAGAG GAACTTGTGC CTTCAGCATC 
TTATTTAAAC TTTTTTCTGA AGGACTGTTT TCTGCCAAAC TTTTCCTTAC GGCTACTTTG 
CATGAGCCAA TCATGCAGCT CCTCGTAGAG GATGAAGACC ACCTGGAAAC AGATCCAAAC 
AAGCTGATAG AGAGATTCTC TCCAGCTCAG CAAGAAAAAC TCTTTGGAGA GAAAGGCTCA 
GATAGATTTA GGCAAAAGGT TCAAGAAATG GTAGATTCCA ATGAGGCTAA ACTAGTGGCT 
TTGGTGAACA AATTTATTGG TTATCTTAAA CAGAATACAT ACTGCTTTCC ACATAGTTTG 
AGGTGGATTG TGTCACAGAT GTACAAAACT CTTTCTTGTG TAGACAGATT GGAGGTTGGG 
GAAGTCAGAG CAATGTGTAC CGATCTTCTG CTGGCTTGCT TCATTTGTCC TGCAGTTGTC 
AATCCAGAGC AGTATGGGAT AATATCTGAT GCTCCTATTA ATGAGGTGGC AAGATTTAAC 
CTGATGCAGG TTGGCCGCCT TTTGCAGCAG CTAGCAATGA CTGGCACTGA AGAAGGAGAT 
CCCCGGACCA AAAATAGCCT TGGAAAATTT GACAAAGGCT GTGTCGCTGC TTTCCTTGAT 
GTTGTAATTG GGGGCCGTGC AGTGGAGACT CCGCCAATGT CCTCTGTTAA TCTTCTGGAA 
GGCTTGAGCA GAACTGTGGT GTATATAAGT TACAGTCAGC TTATTACTCT GGTAAATTTT 
ATGAAGAGTG TGATGTCTGG AGATCAACTG AAAGAAGATA GAATGGCTCT TGACAATTTG 
TTGGCAAACC TGCCCCAGGC TAAGCCAGGG AAGAGCAGCA GTCTGGACAT GACTCCCTAT 
AGCACTCCTC AGATGTCTCC AGCAACCACT CCAGCCAACA AGAAGAATCG ATTACCTATA 
GGACAACAGT TAGCAGCCAT CACTGCCTGG GATTCGTCAG CCACCAATCT TACTGCTCAT 
ATTCCTCTAG TAACCCCATT TGCAACTCGG AGCAGAAGCC GAAGTAATAT GTTAATGGAC 
CTACATATGG ATCATGAAGG ATCATCTCAA GAAACCATCC AGGAGGTACA GCCAGAAGAG 
GTGCTGGTCA TTTCCTTAGG GACAGGCCCT CAGCTCACTC CAGGGATGAT GTCAGAAAAT 
GAGGTTCTAA ACATGCAGCT TTCGGATGGA GGACAAGGAG ATGTCCCTGT TGATGAAAAC 
AAACTCCACG GTAAACCTGA TAAAACCTTG CGCTTTTCCC TCTGCAGTGA TAATCTGGAA 
GGCATATCTG AAGGTCCTTC CAATCGCTCC AATTCGGTGT CTTCTCTCGA CCTGGAGGGA 
GAGTCTGTAT CTGAACTTGG GGCAGGACCT TCTGGGAGTA ACGGAGTTGA AGCACTGCAG 
CTGTTAGAGC ACGAGCAAGC TACAACACAA GATAATCTTG ATGATAAACT GAGGAAGTTT 
GAGATTCGTG ACATGATGGG ACTAACAGAT GACAGGGACA TATCCGAGAC TGTGAGTGAG 
ACCTGGAGCA CTGATGTCCT GGGAAGTGAC TTCGACCCGA ACGTTGATGA AGATCGTTTG 
CAAGAGATTG CAGGTGCAGC AGCAGAGAAC GTGTTAGGTA GTTTGCTGTG CCTTCCAGGT 
TCAGGGTCAG TTCTCCTTGA CCCTTGCACT GGCTCTACCA TATCAGAGAC CACAAGTGAA 
GCTTGGAGTG TAGAGGTATT GCCAAGTGAC TCAGAGGCTC CAGACTTAAA GCAGGAAGAG 
CGCCTCCAAG AGCTGGAGAG CTGTTCTGGA CTGGGCAGCA CATCTGATGA TACGGATGTC 
AGGGAGGTCA GTTCCCGCCC CAGCACACCA GGCCTCAGTG TTGTGTCCGG TATAAGTGCT 
ACATCGGAGG ATATTCCCAA TAAGATCGAA GACCTGAGAT CTGAGTGCAG CTCTGATTTT 
GGGGGCAAAG ATTCTGTAAC TAGTCCAGAT ATGGATGACA TAGCCCATGG CGCTCATCAG 
CTGACCTCTC CTCCCTCTCA GTCAGAGTCT CTGCTTGCCA TGTTTGATCC ACTGTCCTCA 
CATGAAGGGG CCTCTGCTGT GGTAAGGCCA AAGGTTCACT ATGCCCGACC CTCGCATCCA 
CCTCCGGATC CTCCAATCCT GGAAGGTGCA GTGGGAGGAA ATGAGGCCAG GTTGCCAAAC 
TTTGGCTCTC ACGTTTTAAC TGCAGCTGAA ATGGAGGCGT TCAAGCAGAG ACACTCATAC 
CCTGAGAGAT TGGTTCGCAG CAGAAGCTCT GATATAGTGT CTTCTGTGCG GCGGCCCATG 
AGTGACCCCA GCTGGAACCG ACGTCCAGGG AATGAAGAAC TTCCTCCAGC TGCAGCCACT 
GGTGCTACTT CTTTGGTGGC TGCACCTCAC TCGTCATCTT CGTCCCCGAG TAAGGACTCT 
TCTAGAGGAG AGACTGAGGA ACGCAAGGAC AGTGACGATG AGAGGTCAGA CAGGAGCAGG 
CCTTGGTGGA GGAAGCGCTT TGTTTCAGCT ATGCCTAAAG CTCCTATACC ATTTAGAAAG 
AAAGAAAAAC AAGAAAAAGA CAAAGATGAT CTGGGTCCTG ACAGATTCTC AACACTCACA 
GATGAGCCCA GCCCTAGGCT CAGTGCTCAA GCTCAGGTTG CTGAAGATAT TCTGGACAAG 
TACCGGAATG CCATTAAACG GACCAGCCCC AGTGAAGGAG CAATGGCAAA TGATGAGAGT 
GCAGAGGTCA TGGGTGATGG TGAAAGTGCC CACGACTCTC CTCGTGAGGA AGCACTGCAG 
AACATCTCAG CCGACGACCT CCCGGACTCC GCCAGCCAGG CAGCCCACCC TCAGGACTCT 
GCTTTCTCTT ACAGAGATGT AAAAAAGAAG CTGAGGCTCG CACTTTGCTC TGCAGACTCT 
GTGGCCTTCC CAGTGCTAAC TCATTCAACA AGGAATGGAT TGCCAGACCA CACAGACCCA 
GAAGACAATG AAATTGTATG CTTCTTAAAA GTTCAAATAG CTGAAGCAAT TAATTTACAA 
GATAAGAGCT TAATGGCTCA ACTTCAAGAA ACAATGCGTT GTGTGTGCCG CTTTGACAAT 
AGGACTTGTA GGAAGTTGCT GGCATCCATT GCTGAAGACT ACAGAAAAAG AGCTCCGTAT 
ATTGCTTATC TTACCCGCTG CCGACAAGGA CTGCAGACCA CACAGGCTCA CTTGGAAAGG 
CTCTTGCAAA GAGTTTTGCG GGACAAAGAG GTGGCCAATC GGTACTTCAC CACTGTCTGT 
GTGCGGTTAC TGCTTGAGAG CAAAGAAAAG AAAATCAGGG AGTTCATTCA AGACTTTCAG 
AAACTTACTG CTGCTGATGA TAAAACGGCT CAGGTGGAGG ATTTTCTACA GTTCCTGTAT 
GGTGTGATGG CTCAGGATGT CATATGGCAG AATGCCAGTG AGGAGCAGCT GCAGGATGCC 
CAGCTGGCCA TCGAGCGGAG TGTGATGAAT CGGATCTTCA AGCTTGCTTT CTACCCTAAC 
CAGGATGGGG ACATACTTCG TGACCAGGTT CTGCATGAAC ATATCCAGAG ATTATCTAAA 
GTAGTGACTG CAAACCACAG AGCTCTGCAG ATCCCTGAGG TTTATCTCCG TGAGGCACCA 
TGGCCATCTG CACAGTCAGA AATCAGGACA ATAAGTGCTT ACAAAACGCC CCGCGACAAA 
GTGCAGTGCA TCCTGAGGAT GTGCTCCACC ATCATGAACC TCTTGAGCCT GGCCAACGAG 
GACTCTGTCC CCGGGGCAGA TGACTTTGTT CCTGTGTTGG TGTTTGTGTT AATAAAGGCA 
AACCCACCCT GCCTGCTGTC CACTGTGCAG TACATCAGCA GCTTCTATGC CAGCTGTCTG 
TCTGGAGAGG AGTCCTACTG GTGGATGCAG TTCACAGCAG CCGTGGAGTT TATTAAAACC 
ATCGATGACA GAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017174684.1
CDS	353..4810
Translation	MVKLDIHTLAHHLKQERLYVSSEKQLIQRLNADVLKTAEKLYRTAWIAKQQRINLDRLIITSAEASPAECCQHAKILEDTQFVDGYKQLGFQETAYGEFLSRLRENPRLIASSLVAGEKLNQENTQSVIYTVFTSLYGNCIMQEDESYLLQVLRYLIEFELKESDNPRRLLRRGTCAFSILFKLFSEGLFSAKLFLTATLHEPIMQLLVEDEDHLETDPNKLIERFSPAQQEKLFGEKGSDRFRQKVQEMVDSNEAKLVALVNKFIGYLKQNTYCFPHSLRWIVSQMYKTLSCVDRLEVGEVRAMCTDLLLACFICPAVVNPEQYGIISDAPINEVARFNLMQVGRLLQQLAMTGTEEGDPRTKNSLGKFDKGCVAAFLDVVIGGRAVETPPMSSVNLLEGLSRTVVYISYSQLITLVNFMKSVMSGDQLKEDRMALDNLLANLPQAKPGKSSSLDMTPYSTPQMSPATTPANKKNRLPIGQQLAAITAWDSSATNLTAHIPLVTPFATRSRSRSNMLMDLHMDHEGSSQETIQEVQPEEVLVISLGTGPQLTPGMMSENEVLNMQLSDGGQGDVPVDENKLHGKPDKTLRFSLCSDNLEGISEGPSNRSNSVSSLDLEGESVSELGAGPSGSNGVEALQLLEHEQATTQDNLDDKLRKFEIRDMMGLTDDRDISETVSETWSTDVLGSDFDPNVDEDRLQEIAGAAAENVLGSLLCLPGSGSVLLDPCTGSTISETTSEAWSVEVLPSDSEAPDLKQEERLQELESCSGLGSTSDDTDVREVSSRPSTPGLSVVSGISATSEDIPNKIEDLRSECSSDFGGKDSVTSPDMDDIAHGAHQLTSPPSQSESLLAMFDPLSSHEGASAVVRPKVHYARPSHPPPDPPILEGAVGGNEARLPNFGSHVLTAAEMEAFKQRHSYPERLVRSRSSDIVSSVRRPMSDPSWNRRPGNEELPPAAATGATSLVAAPHSSSSSPSKDSSRGETEERKDSDDERSDRSRPWWRKRFVSAMPKAPIPFRKKEKQEKDKDDLGPDRFSTLTDEPSPRLSAQAQVAEDILDKYRNAIKRTSPSEGAMANDESAEVMGDGESAHDSPREEALQNISADDLPDSASQAAHPQDSAFSYRDVKKKLRLALCSADSVAFPVLTHSTRNGLPDHTDPEDNEIVCFLKVQIAEAINLQDKSLMAQLQETMRCVCRFDNRTCRKLLASIAEDYRKRAPYIAYLTRCRQGLQTTQAHLERLLQRVLRDKEVANRYFTTVCVRLLLESKEKKIREFIQDFQKLTAADDKTAQVEDFLQFLYGVMAQDVIWQNASEEQLQDAQLAIERSVMNRIFKLAFYPNQDGDILRDQVLHEHIQRLSKVVTANHRALQIPEVYLREAPWPSAQSEIRTISAYKTPRDKVQCILRMCSTIMNLLSLANEDSVPGADDFVPVLVFVLIKANPPCLLSTVQYISSFYASCLSGEESYWWMQFTAAVEFIKTIDDRK

Target ORF information:

RefSeq Version	XM_017319195.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus GTPase activating protein and VPS9 domains 1 (Gapvd1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319195.2

ORF Insert Sequence:

ATGGTGAAGC TAGATATTCA CACATTGGCC CACCACCTCA AGCAGGAACG CTTATATGTG 
AGCTCTGAGA AACAGCTCAT TCAGAGGCTA AATGCCGATG TACTGAAGAC AGCTGAAAAG 
CTGTATCGAA CAGCGTGGAT TGCAAAGCAA CAGAGGATTA ATTTGGATCG TCTTATTATA 
ACCAGTGCTG AAGCTTCCCC TGCTGAGTGT TGCCAGCATG CCAAGATTTT GGAAGATACA 
CAGTTTGTTG ATGGATATAA GCAACTGGGA TTTCAGGAAA CAGCTTATGG AGAATTCCTG 
AGTCGGTTAA GAGAAAATCC ACGACTTATT GCCTCCTCTT TGGTTGCTGG AGAAAAACTC 
AATCAGGAGA ACACACAAAG TGTTATCTAC ACTGTTTTTA CCTCCTTGTA TGGCAACTGT 
ATTATGCAAG AAGATGAAAG CTACCTCCTT CAGGTTTTAC GATACTTGAT TGAATTTGAA 
CTTAAAGAAA GTGACAACCC CAGGCGCCTT TTGAGGAGAG GAACTTGTGC CTTCAGCATC 
TTATTTAAAC TTTTTTCTGA AGGACTGTTT TCTGCCAAAC TTTTCCTTAC GGCTACTTTG 
CATGAGCCAA TCATGCAGCT CCTCGTAGAG GATGAAGACC ACCTGGAAAC AGATCCAAAC 
AAGCTGATAG AGAGATTCTC TCCAGCTCAG CAAGAAAAAC TCTTTGGAGA GAAAGGCTCA 
GATAGATTTA GGCAAAAGGT TCAAGAAATG GTAGATTCCA ATGAGGCTAA ACTAGTGGCT 
TTGGTGAACA AATTTATTGG TTATCTTAAA CAGAATACAT ACTGCTTTCC ACATAGTTTG 
AGGTGGATTG TGTCACAGAT GTACAAAACT CTTTCTTGTG TAGACAGATT GGAGGTTGGG 
GAAGTCAGAG CAATGTGTAC CGATCTTCTG CTGGCTTGCT TCATTTGTCC TGCAGTTGTC 
AATCCAGAGC AGTATGGGAT AATATCTGAT GCTCCTATTA ATGAGGTGGC AAGATTTAAC 
CTGATGCAGG TTGGCCGCCT TTTGCAGCAG CTAGCAATGA CTGGCACTGA AGAAGGAGAT 
CCCCGGACCA AAAATAGCCT TGGAAAATTT GACAAAGGCT GTGTCGCTGC TTTCCTTGAT 
GTTGTAATTG GGGGCCGTGC AGTGGAGACT CCGCCAATGT CCTCTGTTAA TCTTCTGGAA 
GGCTTGAGCA GAACTGTGGT GTATATAAGT TACAGTCAGC TTATTACTCT GGTAAATTTT 
ATGAAGAGTG TGATGTCTGG AGATCAACTG AAAGAAGATA GAATGGCTCT TGACAATTTG 
TTGGCAAACC TGCCCCAGGC TAAGCCAGGG AAGAGCAGCA GTCTGGACAT GACTCCCTAT 
AGCACTCCTC AGATGTCTCC AGCAACCACT CCAGCCAACA AGAAGAATCG ATTACCTATA 
GGACAACAGT TAGCAGCCAT CACTGCCTGG GATTCGTCAG CCACCAATCT TACTGCTCAT 
ATTCCTCTAG TAACCCCATT TGCAACTCGG AGCAGAAGCC GAAGTAATAT GTTAATGGAC 
CTACATATGG ATCATGAAGG ATCATCTCAA GAAACCATCC AGGAGGTACA GCCAGAAGAG 
GTGCTGGTCA TTTCCTTAGG GACAGGCCCT CAGCTCACTC CAGGGATGAT GTCAGAAAAT 
GAGGTTCTAA ACATGCAGCT TTCGGATGGA GGACAAGGAG ATGTCCCTGT TGATGAAAAC 
AAACTCCACG GTAAACCTGA TAAAACCTTG CGCTTTTCCC TCTGCAGTGA TAATCTGGAA 
GGCATATCTG AAGGTCCTTC CAATCGCTCC AATTCGGTGT CTTCTCTCGA CCTGGAGGGA 
GAGTCTGTAT CTGAACTTGG GGCAGGACCT TCTGGGAGTA ACGGAGTTGA AGCACTGCAG 
CTGTTAGAGC ACGAGCAAGC TACAACACAA GATAATCTTG ATGATAAACT GAGGAAGTTT 
GAGATTCGTG ACATGATGGG ACTAACAGAT GACAGGGACA TATCCGAGAC TGTGAGTGAG 
ACCTGGAGCA CTGATGTCCT GGGAAGTGAC TTCGACCCGA ACGTTGATGA AGATCGTTTG 
CAAGAGATTG CAGGTGCAGC AGCAGAGAAC GTGTTAGGTA GTTTGCTGTG CCTTCCAGGT 
TCAGGGTCAG TTCTCCTTGA CCCTTGCACT GGCTCTACCA TATCAGAGAC CACAAGTGAA 
GCTTGGAGTG TAGAGGTATT GCCAAGTGAC TCAGAGGCTC CAGACTTAAA GCAGGAAGAG 
CGCCTCCAAG AGCTGGAGAG CTGTTCTGGA CTGGGCAGCA CATCTGATGA TACGGATGTC 
AGGGAGGTCA GTTCCCGCCC CAGCACACCA GGCCTCAGTG TTGTGTCCGG TATAAGTGCT 
ACATCGGAGG ATATTCCCAA TAAGATCGAA GACCTGAGAT CTGAGTGCAG CTCTGATTTT 
GGGGGCAAAG ATTCTGTAAC TAGTCCAGAT ATGGATGACA TAGCCCATGG CGCTCATCAG 
CTGACCTCTC CTCCCTCTCA GTCAGAGTCT CTGCTTGCCA TGTTTGATCC ACTGTCCTCA 
CATGAAGGGG CCTCTGCTGT GGTAAGGCCA AAGGTTCACT ATGCCCGACC CTCGCATCCA 
CCTCCGGATC CTCCAATCCT GGAAGGTGCA GTGGGAGGAA ATGAGGCCAG GTTGCCAAAC 
TTTGGCTCTC ACGTTTTAAC TGCAGCTGAA ATGGAGGCGT TCAAGCAGAG ACACTCATAC 
CCTGAGAGAT TGGTTCGCAG CAGAAGCTCT GATATAGTGT CTTCTGTGCG GCGGCCCATG 
AGTGACCCCA GCTGGAACCG ACGTCCAGGG AATGAAGAAC TTCCTCCAGC TGCAGCCACT 
GGTGCTACTT CTTTGGTGGC TGCACCTCAC TCGTCATCTT CGTCCCCGAG TAAGGACTCT 
TCTAGAGGAG AGACTGAGGA ACGCAAGGAC AGTGACGATG AGAGGTCAGA CAGGAGCAGG 
CCTTGGTGGA GGAAGCGCTT TGTTTCAGCT ATGCCTAAAG CTCCTATACC ATTTAGAAAG 
AAAGAAAAAC AAGAAAAAGA CAAAGATGAT CTGGGTCCTG ACAGATTCTC AACACTCACA 
GATGAGCCCA GCCCTAGGCT CAGTGCTCAA GCTCAGGTTG CTGAAGATAT TCTGGACAAG 
TACCGGAATG CCATTAAACG GACCAGCCCC AGTGAAGGAG CAATGGCAAA TGATGAGAGT 
GCAGAGGTCA TGGGTGATGG TGAAAGTGCC CACGACTCTC CTCGTGAGGA AGCACTGCAG 
AACATCTCAG CCGACGACCT CCCGGACTCC GCCAGCCAGG CAGCCCACCC TCAGGACTCT 
GCTTTCTCTT ACAGAGATGT AAAAAAGAAG CTGAGGCTCG CACTTTGCTC TGCAGACTCT 
GTGGCCTTCC CAGTGCTAAC TCATTCAACA AGGAATGGAT TGCCAGACCA CACAGACCCA 
GAAGACAATG AAATTGTATG CTTCTTAAAA GTTCAAATAG CTGAAGCAAT TAATTTACAA 
GATAAGAGCT TAATGGCTCA ACTTCAAGAA ACAATGCGTT GTGTGTGCCG CTTTGACAAT 
AGGACTTGTA GGAAGTTGCT GGCATCCATT GCTGAAGACT ACAGAAAAAG AGCTCCGTAT 
ATTGCTTATC TTACCCGCTG CCGACAAGGA CTGCAGACCA CACAGGCTCA CTTGGAAAGG 
CTCTTGCAAA GAGTTTTGCG GGACAAAGAG GTGGCCAATC GGTACTTCAC CACTGTCTGT 
GTGCGGTTAC TGCTTGAGAG CAAAGAAAAG AAAATCAGGG AGTTCATTCA AGACTTTCAG 
AAACTTACTG CTGCTGATGA TAAAACGGCT CAGGTGGAGG ATTTTCTACA GTTCCTGTAT 
GGTGTGATGG CTCAGGATGT CATATGGCAG AATGCCAGTG AGGAGCAGCT GCAGGATGCC 
CAGCTGGCCA TCGAGCGGAG TGTGATGAAT CGGATCTTCA AGCTTGCTTT CTACCCTAAC 
CAGGATGGGG ACATACTTCG TGACCAGGTT CTGCATGAAC ATATCCAGAG ATTATCTAAA 
GTAGTGACTG CAAACCACAG AGCTCTGCAG ATCCCTGAGG TTTATCTCCG TGAGGCACCA 
TGGCCATCTG CACAGTCAGA AATCAGGACA ATAAGTGCTT ACAAAACGCC CCGCGACAAA 
GTGCAGTGCA TCCTGAGGAT GTGCTCCACC ATCATGAACC TCTTGAGCCT GGCCAACGAG 
GACTCTGTCC CCGGGGCAGA TGACTTTGTT CCTGTGTTGG TGTTTGTGTT AATAAAGGCA 
AACCCACCCT GCCTGCTGTC CACTGTGCAG TACATCAGCA GCTTCTATGC CAGCTGTCTG 
TCTGGAGAGG AGTCCTACTG GTGGATGCAG TTCACAGCAG CCGTGGAGTT TATTAAAACC 
ATCGATGACA GAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAPVD1 ( XM_017319196.2 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_017174685.1 Mus musculus GTPase activating protein and VPS9 domains 1 (Gapvd1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu69988
Clone ID Related Accession (Same CDS sequence)	XM_017319195.2 , XM_017319196.2 , XM_011239145.1 , XM_017319195.1 , XM_017319196.1
Accession Version	XM_017319196.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4458bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	GTPase-activating protein and VPS9 domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017319196.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGC TAGATATTCA CACATTGGCC CACCACCTCA AGCAGGAACG CTTATATGTG 
AGCTCTGAGA AACAGCTCAT TCAGAGGCTA AATGCCGATG TACTGAAGAC AGCTGAAAAG 
CTGTATCGAA CAGCGTGGAT TGCAAAGCAA CAGAGGATTA ATTTGGATCG TCTTATTATA 
ACCAGTGCTG AAGCTTCCCC TGCTGAGTGT TGCCAGCATG CCAAGATTTT GGAAGATACA 
CAGTTTGTTG ATGGATATAA GCAACTGGGA TTTCAGGAAA CAGCTTATGG AGAATTCCTG 
AGTCGGTTAA GAGAAAATCC ACGACTTATT GCCTCCTCTT TGGTTGCTGG AGAAAAACTC 
AATCAGGAGA ACACACAAAG TGTTATCTAC ACTGTTTTTA CCTCCTTGTA TGGCAACTGT 
ATTATGCAAG AAGATGAAAG CTACCTCCTT CAGGTTTTAC GATACTTGAT TGAATTTGAA 
CTTAAAGAAA GTGACAACCC CAGGCGCCTT TTGAGGAGAG GAACTTGTGC CTTCAGCATC 
TTATTTAAAC TTTTTTCTGA AGGACTGTTT TCTGCCAAAC TTTTCCTTAC GGCTACTTTG 
CATGAGCCAA TCATGCAGCT CCTCGTAGAG GATGAAGACC ACCTGGAAAC AGATCCAAAC 
AAGCTGATAG AGAGATTCTC TCCAGCTCAG CAAGAAAAAC TCTTTGGAGA GAAAGGCTCA 
GATAGATTTA GGCAAAAGGT TCAAGAAATG GTAGATTCCA ATGAGGCTAA ACTAGTGGCT 
TTGGTGAACA AATTTATTGG TTATCTTAAA CAGAATACAT ACTGCTTTCC ACATAGTTTG 
AGGTGGATTG TGTCACAGAT GTACAAAACT CTTTCTTGTG TAGACAGATT GGAGGTTGGG 
GAAGTCAGAG CAATGTGTAC CGATCTTCTG CTGGCTTGCT TCATTTGTCC TGCAGTTGTC 
AATCCAGAGC AGTATGGGAT AATATCTGAT GCTCCTATTA ATGAGGTGGC AAGATTTAAC 
CTGATGCAGG TTGGCCGCCT TTTGCAGCAG CTAGCAATGA CTGGCACTGA AGAAGGAGAT 
CCCCGGACCA AAAATAGCCT TGGAAAATTT GACAAAGGCT GTGTCGCTGC TTTCCTTGAT 
GTTGTAATTG GGGGCCGTGC AGTGGAGACT CCGCCAATGT CCTCTGTTAA TCTTCTGGAA 
GGCTTGAGCA GAACTGTGGT GTATATAAGT TACAGTCAGC TTATTACTCT GGTAAATTTT 
ATGAAGAGTG TGATGTCTGG AGATCAACTG AAAGAAGATA GAATGGCTCT TGACAATTTG 
TTGGCAAACC TGCCCCAGGC TAAGCCAGGG AAGAGCAGCA GTCTGGACAT GACTCCCTAT 
AGCACTCCTC AGATGTCTCC AGCAACCACT CCAGCCAACA AGAAGAATCG ATTACCTATA 
GGACAACAGT TAGCAGCCAT CACTGCCTGG GATTCGTCAG CCACCAATCT TACTGCTCAT 
ATTCCTCTAG TAACCCCATT TGCAACTCGG AGCAGAAGCC GAAGTAATAT GTTAATGGAC 
CTACATATGG ATCATGAAGG ATCATCTCAA GAAACCATCC AGGAGGTACA GCCAGAAGAG 
GTGCTGGTCA TTTCCTTAGG GACAGGCCCT CAGCTCACTC CAGGGATGAT GTCAGAAAAT 
GAGGTTCTAA ACATGCAGCT TTCGGATGGA GGACAAGGAG ATGTCCCTGT TGATGAAAAC 
AAACTCCACG GTAAACCTGA TAAAACCTTG CGCTTTTCCC TCTGCAGTGA TAATCTGGAA 
GGCATATCTG AAGGTCCTTC CAATCGCTCC AATTCGGTGT CTTCTCTCGA CCTGGAGGGA 
GAGTCTGTAT CTGAACTTGG GGCAGGACCT TCTGGGAGTA ACGGAGTTGA AGCACTGCAG 
CTGTTAGAGC ACGAGCAAGC TACAACACAA GATAATCTTG ATGATAAACT GAGGAAGTTT 
GAGATTCGTG ACATGATGGG ACTAACAGAT GACAGGGACA TATCCGAGAC TGTGAGTGAG 
ACCTGGAGCA CTGATGTCCT GGGAAGTGAC TTCGACCCGA ACGTTGATGA AGATCGTTTG 
CAAGAGATTG CAGGTGCAGC AGCAGAGAAC GTGTTAGGTA GTTTGCTGTG CCTTCCAGGT 
TCAGGGTCAG TTCTCCTTGA CCCTTGCACT GGCTCTACCA TATCAGAGAC CACAAGTGAA 
GCTTGGAGTG TAGAGGTATT GCCAAGTGAC TCAGAGGCTC CAGACTTAAA GCAGGAAGAG 
CGCCTCCAAG AGCTGGAGAG CTGTTCTGGA CTGGGCAGCA CATCTGATGA TACGGATGTC 
AGGGAGGTCA GTTCCCGCCC CAGCACACCA GGCCTCAGTG TTGTGTCCGG TATAAGTGCT 
ACATCGGAGG ATATTCCCAA TAAGATCGAA GACCTGAGAT CTGAGTGCAG CTCTGATTTT 
GGGGGCAAAG ATTCTGTAAC TAGTCCAGAT ATGGATGACA TAGCCCATGG CGCTCATCAG 
CTGACCTCTC CTCCCTCTCA GTCAGAGTCT CTGCTTGCCA TGTTTGATCC ACTGTCCTCA 
CATGAAGGGG CCTCTGCTGT GGTAAGGCCA AAGGTTCACT ATGCCCGACC CTCGCATCCA 
CCTCCGGATC CTCCAATCCT GGAAGGTGCA GTGGGAGGAA ATGAGGCCAG GTTGCCAAAC 
TTTGGCTCTC ACGTTTTAAC TGCAGCTGAA ATGGAGGCGT TCAAGCAGAG ACACTCATAC 
CCTGAGAGAT TGGTTCGCAG CAGAAGCTCT GATATAGTGT CTTCTGTGCG GCGGCCCATG 
AGTGACCCCA GCTGGAACCG ACGTCCAGGG AATGAAGAAC TTCCTCCAGC TGCAGCCACT 
GGTGCTACTT CTTTGGTGGC TGCACCTCAC TCGTCATCTT CGTCCCCGAG TAAGGACTCT 
TCTAGAGGAG AGACTGAGGA ACGCAAGGAC AGTGACGATG AGAGGTCAGA CAGGAGCAGG 
CCTTGGTGGA GGAAGCGCTT TGTTTCAGCT ATGCCTAAAG CTCCTATACC ATTTAGAAAG 
AAAGAAAAAC AAGAAAAAGA CAAAGATGAT CTGGGTCCTG ACAGATTCTC AACACTCACA 
GATGAGCCCA GCCCTAGGCT CAGTGCTCAA GCTCAGGTTG CTGAAGATAT TCTGGACAAG 
TACCGGAATG CCATTAAACG GACCAGCCCC AGTGAAGGAG CAATGGCAAA TGATGAGAGT 
GCAGAGGTCA TGGGTGATGG TGAAAGTGCC CACGACTCTC CTCGTGAGGA AGCACTGCAG 
AACATCTCAG CCGACGACCT CCCGGACTCC GCCAGCCAGG CAGCCCACCC TCAGGACTCT 
GCTTTCTCTT ACAGAGATGT AAAAAAGAAG CTGAGGCTCG CACTTTGCTC TGCAGACTCT 
GTGGCCTTCC CAGTGCTAAC TCATTCAACA AGGAATGGAT TGCCAGACCA CACAGACCCA 
GAAGACAATG AAATTGTATG CTTCTTAAAA GTTCAAATAG CTGAAGCAAT TAATTTACAA 
GATAAGAGCT TAATGGCTCA ACTTCAAGAA ACAATGCGTT GTGTGTGCCG CTTTGACAAT 
AGGACTTGTA GGAAGTTGCT GGCATCCATT GCTGAAGACT ACAGAAAAAG AGCTCCGTAT 
ATTGCTTATC TTACCCGCTG CCGACAAGGA CTGCAGACCA CACAGGCTCA CTTGGAAAGG 
CTCTTGCAAA GAGTTTTGCG GGACAAAGAG GTGGCCAATC GGTACTTCAC CACTGTCTGT 
GTGCGGTTAC TGCTTGAGAG CAAAGAAAAG AAAATCAGGG AGTTCATTCA AGACTTTCAG 
AAACTTACTG CTGCTGATGA TAAAACGGCT CAGGTGGAGG ATTTTCTACA GTTCCTGTAT 
GGTGTGATGG CTCAGGATGT CATATGGCAG AATGCCAGTG AGGAGCAGCT GCAGGATGCC 
CAGCTGGCCA TCGAGCGGAG TGTGATGAAT CGGATCTTCA AGCTTGCTTT CTACCCTAAC 
CAGGATGGGG ACATACTTCG TGACCAGGTT CTGCATGAAC ATATCCAGAG ATTATCTAAA 
GTAGTGACTG CAAACCACAG AGCTCTGCAG ATCCCTGAGG TTTATCTCCG TGAGGCACCA 
TGGCCATCTG CACAGTCAGA AATCAGGACA ATAAGTGCTT ACAAAACGCC CCGCGACAAA 
GTGCAGTGCA TCCTGAGGAT GTGCTCCACC ATCATGAACC TCTTGAGCCT GGCCAACGAG 
GACTCTGTCC CCGGGGCAGA TGACTTTGTT CCTGTGTTGG TGTTTGTGTT AATAAAGGCA 
AACCCACCCT GCCTGCTGTC CACTGTGCAG TACATCAGCA GCTTCTATGC CAGCTGTCTG 
TCTGGAGAGG AGTCCTACTG GTGGATGCAG TTCACAGCAG CCGTGGAGTT TATTAAAACC 
ATCGATGACA GAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017174685.1
CDS	246..4703
Translation	MVKLDIHTLAHHLKQERLYVSSEKQLIQRLNADVLKTAEKLYRTAWIAKQQRINLDRLIITSAEASPAECCQHAKILEDTQFVDGYKQLGFQETAYGEFLSRLRENPRLIASSLVAGEKLNQENTQSVIYTVFTSLYGNCIMQEDESYLLQVLRYLIEFELKESDNPRRLLRRGTCAFSILFKLFSEGLFSAKLFLTATLHEPIMQLLVEDEDHLETDPNKLIERFSPAQQEKLFGEKGSDRFRQKVQEMVDSNEAKLVALVNKFIGYLKQNTYCFPHSLRWIVSQMYKTLSCVDRLEVGEVRAMCTDLLLACFICPAVVNPEQYGIISDAPINEVARFNLMQVGRLLQQLAMTGTEEGDPRTKNSLGKFDKGCVAAFLDVVIGGRAVETPPMSSVNLLEGLSRTVVYISYSQLITLVNFMKSVMSGDQLKEDRMALDNLLANLPQAKPGKSSSLDMTPYSTPQMSPATTPANKKNRLPIGQQLAAITAWDSSATNLTAHIPLVTPFATRSRSRSNMLMDLHMDHEGSSQETIQEVQPEEVLVISLGTGPQLTPGMMSENEVLNMQLSDGGQGDVPVDENKLHGKPDKTLRFSLCSDNLEGISEGPSNRSNSVSSLDLEGESVSELGAGPSGSNGVEALQLLEHEQATTQDNLDDKLRKFEIRDMMGLTDDRDISETVSETWSTDVLGSDFDPNVDEDRLQEIAGAAAENVLGSLLCLPGSGSVLLDPCTGSTISETTSEAWSVEVLPSDSEAPDLKQEERLQELESCSGLGSTSDDTDVREVSSRPSTPGLSVVSGISATSEDIPNKIEDLRSECSSDFGGKDSVTSPDMDDIAHGAHQLTSPPSQSESLLAMFDPLSSHEGASAVVRPKVHYARPSHPPPDPPILEGAVGGNEARLPNFGSHVLTAAEMEAFKQRHSYPERLVRSRSSDIVSSVRRPMSDPSWNRRPGNEELPPAAATGATSLVAAPHSSSSSPSKDSSRGETEERKDSDDERSDRSRPWWRKRFVSAMPKAPIPFRKKEKQEKDKDDLGPDRFSTLTDEPSPRLSAQAQVAEDILDKYRNAIKRTSPSEGAMANDESAEVMGDGESAHDSPREEALQNISADDLPDSASQAAHPQDSAFSYRDVKKKLRLALCSADSVAFPVLTHSTRNGLPDHTDPEDNEIVCFLKVQIAEAINLQDKSLMAQLQETMRCVCRFDNRTCRKLLASIAEDYRKRAPYIAYLTRCRQGLQTTQAHLERLLQRVLRDKEVANRYFTTVCVRLLLESKEKKIREFIQDFQKLTAADDKTAQVEDFLQFLYGVMAQDVIWQNASEEQLQDAQLAIERSVMNRIFKLAFYPNQDGDILRDQVLHEHIQRLSKVVTANHRALQIPEVYLREAPWPSAQSEIRTISAYKTPRDKVQCILRMCSTIMNLLSLANEDSVPGADDFVPVLVFVLIKANPPCLLSTVQYISSFYASCLSGEESYWWMQFTAAVEFIKTIDDRK

Target ORF information:

RefSeq Version	XM_017319196.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus GTPase activating protein and VPS9 domains 1 (Gapvd1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319196.2

ORF Insert Sequence:

ATGGTGAAGC TAGATATTCA CACATTGGCC CACCACCTCA AGCAGGAACG CTTATATGTG 
AGCTCTGAGA AACAGCTCAT TCAGAGGCTA AATGCCGATG TACTGAAGAC AGCTGAAAAG 
CTGTATCGAA CAGCGTGGAT TGCAAAGCAA CAGAGGATTA ATTTGGATCG TCTTATTATA 
ACCAGTGCTG AAGCTTCCCC TGCTGAGTGT TGCCAGCATG CCAAGATTTT GGAAGATACA 
CAGTTTGTTG ATGGATATAA GCAACTGGGA TTTCAGGAAA CAGCTTATGG AGAATTCCTG 
AGTCGGTTAA GAGAAAATCC ACGACTTATT GCCTCCTCTT TGGTTGCTGG AGAAAAACTC 
AATCAGGAGA ACACACAAAG TGTTATCTAC ACTGTTTTTA CCTCCTTGTA TGGCAACTGT 
ATTATGCAAG AAGATGAAAG CTACCTCCTT CAGGTTTTAC GATACTTGAT TGAATTTGAA 
CTTAAAGAAA GTGACAACCC CAGGCGCCTT TTGAGGAGAG GAACTTGTGC CTTCAGCATC 
TTATTTAAAC TTTTTTCTGA AGGACTGTTT TCTGCCAAAC TTTTCCTTAC GGCTACTTTG 
CATGAGCCAA TCATGCAGCT CCTCGTAGAG GATGAAGACC ACCTGGAAAC AGATCCAAAC 
AAGCTGATAG AGAGATTCTC TCCAGCTCAG CAAGAAAAAC TCTTTGGAGA GAAAGGCTCA 
GATAGATTTA GGCAAAAGGT TCAAGAAATG GTAGATTCCA ATGAGGCTAA ACTAGTGGCT 
TTGGTGAACA AATTTATTGG TTATCTTAAA CAGAATACAT ACTGCTTTCC ACATAGTTTG 
AGGTGGATTG TGTCACAGAT GTACAAAACT CTTTCTTGTG TAGACAGATT GGAGGTTGGG 
GAAGTCAGAG CAATGTGTAC CGATCTTCTG CTGGCTTGCT TCATTTGTCC TGCAGTTGTC 
AATCCAGAGC AGTATGGGAT AATATCTGAT GCTCCTATTA ATGAGGTGGC AAGATTTAAC 
CTGATGCAGG TTGGCCGCCT TTTGCAGCAG CTAGCAATGA CTGGCACTGA AGAAGGAGAT 
CCCCGGACCA AAAATAGCCT TGGAAAATTT GACAAAGGCT GTGTCGCTGC TTTCCTTGAT 
GTTGTAATTG GGGGCCGTGC AGTGGAGACT CCGCCAATGT CCTCTGTTAA TCTTCTGGAA 
GGCTTGAGCA GAACTGTGGT GTATATAAGT TACAGTCAGC TTATTACTCT GGTAAATTTT 
ATGAAGAGTG TGATGTCTGG AGATCAACTG AAAGAAGATA GAATGGCTCT TGACAATTTG 
TTGGCAAACC TGCCCCAGGC TAAGCCAGGG AAGAGCAGCA GTCTGGACAT GACTCCCTAT 
AGCACTCCTC AGATGTCTCC AGCAACCACT CCAGCCAACA AGAAGAATCG ATTACCTATA 
GGACAACAGT TAGCAGCCAT CACTGCCTGG GATTCGTCAG CCACCAATCT TACTGCTCAT 
ATTCCTCTAG TAACCCCATT TGCAACTCGG AGCAGAAGCC GAAGTAATAT GTTAATGGAC 
CTACATATGG ATCATGAAGG ATCATCTCAA GAAACCATCC AGGAGGTACA GCCAGAAGAG 
GTGCTGGTCA TTTCCTTAGG GACAGGCCCT CAGCTCACTC CAGGGATGAT GTCAGAAAAT 
GAGGTTCTAA ACATGCAGCT TTCGGATGGA GGACAAGGAG ATGTCCCTGT TGATGAAAAC 
AAACTCCACG GTAAACCTGA TAAAACCTTG CGCTTTTCCC TCTGCAGTGA TAATCTGGAA 
GGCATATCTG AAGGTCCTTC CAATCGCTCC AATTCGGTGT CTTCTCTCGA CCTGGAGGGA 
GAGTCTGTAT CTGAACTTGG GGCAGGACCT TCTGGGAGTA ACGGAGTTGA AGCACTGCAG 
CTGTTAGAGC ACGAGCAAGC TACAACACAA GATAATCTTG ATGATAAACT GAGGAAGTTT 
GAGATTCGTG ACATGATGGG ACTAACAGAT GACAGGGACA TATCCGAGAC TGTGAGTGAG 
ACCTGGAGCA CTGATGTCCT GGGAAGTGAC TTCGACCCGA ACGTTGATGA AGATCGTTTG 
CAAGAGATTG CAGGTGCAGC AGCAGAGAAC GTGTTAGGTA GTTTGCTGTG CCTTCCAGGT 
TCAGGGTCAG TTCTCCTTGA CCCTTGCACT GGCTCTACCA TATCAGAGAC CACAAGTGAA 
GCTTGGAGTG TAGAGGTATT GCCAAGTGAC TCAGAGGCTC CAGACTTAAA GCAGGAAGAG 
CGCCTCCAAG AGCTGGAGAG CTGTTCTGGA CTGGGCAGCA CATCTGATGA TACGGATGTC 
AGGGAGGTCA GTTCCCGCCC CAGCACACCA GGCCTCAGTG TTGTGTCCGG TATAAGTGCT 
ACATCGGAGG ATATTCCCAA TAAGATCGAA GACCTGAGAT CTGAGTGCAG CTCTGATTTT 
GGGGGCAAAG ATTCTGTAAC TAGTCCAGAT ATGGATGACA TAGCCCATGG CGCTCATCAG 
CTGACCTCTC CTCCCTCTCA GTCAGAGTCT CTGCTTGCCA TGTTTGATCC ACTGTCCTCA 
CATGAAGGGG CCTCTGCTGT GGTAAGGCCA AAGGTTCACT ATGCCCGACC CTCGCATCCA 
CCTCCGGATC CTCCAATCCT GGAAGGTGCA GTGGGAGGAA ATGAGGCCAG GTTGCCAAAC 
TTTGGCTCTC ACGTTTTAAC TGCAGCTGAA ATGGAGGCGT TCAAGCAGAG ACACTCATAC 
CCTGAGAGAT TGGTTCGCAG CAGAAGCTCT GATATAGTGT CTTCTGTGCG GCGGCCCATG 
AGTGACCCCA GCTGGAACCG ACGTCCAGGG AATGAAGAAC TTCCTCCAGC TGCAGCCACT 
GGTGCTACTT CTTTGGTGGC TGCACCTCAC TCGTCATCTT CGTCCCCGAG TAAGGACTCT 
TCTAGAGGAG AGACTGAGGA ACGCAAGGAC AGTGACGATG AGAGGTCAGA CAGGAGCAGG 
CCTTGGTGGA GGAAGCGCTT TGTTTCAGCT ATGCCTAAAG CTCCTATACC ATTTAGAAAG 
AAAGAAAAAC AAGAAAAAGA CAAAGATGAT CTGGGTCCTG ACAGATTCTC AACACTCACA 
GATGAGCCCA GCCCTAGGCT CAGTGCTCAA GCTCAGGTTG CTGAAGATAT TCTGGACAAG 
TACCGGAATG CCATTAAACG GACCAGCCCC AGTGAAGGAG CAATGGCAAA TGATGAGAGT 
GCAGAGGTCA TGGGTGATGG TGAAAGTGCC CACGACTCTC CTCGTGAGGA AGCACTGCAG 
AACATCTCAG CCGACGACCT CCCGGACTCC GCCAGCCAGG CAGCCCACCC TCAGGACTCT 
GCTTTCTCTT ACAGAGATGT AAAAAAGAAG CTGAGGCTCG CACTTTGCTC TGCAGACTCT 
GTGGCCTTCC CAGTGCTAAC TCATTCAACA AGGAATGGAT TGCCAGACCA CACAGACCCA 
GAAGACAATG AAATTGTATG CTTCTTAAAA GTTCAAATAG CTGAAGCAAT TAATTTACAA 
GATAAGAGCT TAATGGCTCA ACTTCAAGAA ACAATGCGTT GTGTGTGCCG CTTTGACAAT 
AGGACTTGTA GGAAGTTGCT GGCATCCATT GCTGAAGACT ACAGAAAAAG AGCTCCGTAT 
ATTGCTTATC TTACCCGCTG CCGACAAGGA CTGCAGACCA CACAGGCTCA CTTGGAAAGG 
CTCTTGCAAA GAGTTTTGCG GGACAAAGAG GTGGCCAATC GGTACTTCAC CACTGTCTGT 
GTGCGGTTAC TGCTTGAGAG CAAAGAAAAG AAAATCAGGG AGTTCATTCA AGACTTTCAG 
AAACTTACTG CTGCTGATGA TAAAACGGCT CAGGTGGAGG ATTTTCTACA GTTCCTGTAT 
GGTGTGATGG CTCAGGATGT CATATGGCAG AATGCCAGTG AGGAGCAGCT GCAGGATGCC 
CAGCTGGCCA TCGAGCGGAG TGTGATGAAT CGGATCTTCA AGCTTGCTTT CTACCCTAAC 
CAGGATGGGG ACATACTTCG TGACCAGGTT CTGCATGAAC ATATCCAGAG ATTATCTAAA 
GTAGTGACTG CAAACCACAG AGCTCTGCAG ATCCCTGAGG TTTATCTCCG TGAGGCACCA 
TGGCCATCTG CACAGTCAGA AATCAGGACA ATAAGTGCTT ACAAAACGCC CCGCGACAAA 
GTGCAGTGCA TCCTGAGGAT GTGCTCCACC ATCATGAACC TCTTGAGCCT GGCCAACGAG 
GACTCTGTCC CCGGGGCAGA TGACTTTGTT CCTGTGTTGG TGTTTGTGTT AATAAAGGCA 
AACCCACCCT GCCTGCTGTC CACTGTGCAG TACATCAGCA GCTTCTATGC CAGCTGTCTG 
TCTGGAGAGG AGTCCTACTG GTGGATGCAG TTCACAGCAG CCGTGGAGTT TATTAAAACC 
ATCGATGACA GAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAPVD1 ( XM_030251954.1 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_030107814.1 Mus musculus GTPase activating protein and VPS9 domains 1 (Gapvd1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu69989
Clone ID Related Accession (Same CDS sequence)	XM_030251954.1 , XM_011239147.1
Accession Version	XM_030251954.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	GTPase-activating protein and VPS9 domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGC TAGATATTCA CACATTGGCC CACCACCTCA AGCAGGAACG CTTATATGTG 
AGCTCTGAGA AACAGCTCAT TCAGAGGCTA AATGCCGATG TACTGAAGAC AGCTGAAAAG 
CTGTATCGAA CAGCGTGGAT TGCAAAGCAA CAGAGGATTA ATTTGGATCG TCTTATTATA 
ACCAGTGCTG AAGCTTCCCC TGCTGAGTGT TGCCAGCATG CCAAGATTTT GGAAGATACA 
CAGTTTGTTG ATGGATATAA GCAACTGGGA TTTCAGGAAA CAGCTTATGG AGAATTCCTG 
AGTCGGTTAA GAGAAAATCC ACGACTTATT GCCTCCTCTT TGGTTGCTGG AGAAAAACTC 
AATCAGGAGA ACACACAAAG TGTTATCTAC ACTGTTTTTA CCTCCTTGTA TGGCAACTGT 
ATTATGCAAG AAGATGAAAG CTACCTCCTT CAGGTTTTAC GATACTTGAT TGAATTTGAA 
CTTAAAGAAA GTGACAACCC CAGGCGCCTT TTGAGGAGAG GAACTTGTGC CTTCAGCATC 
TTATTTAAAC TTTTTTCTGA AGGACTGTTT TCTGCCAAAC TTTTCCTTAC GGCTACTTTG 
CATGAGCCAA TCATGCAGCT CCTCGTAGAG GATGAAGACC ACCTGGAAAC AGATCCAAAC 
AAGCTGATAG AGAGATTCTC TCCAGCTCAG CAAGAAAAAC TCTTTGGAGA GAAAGGCTCA 
GATAGATTTA GGCAAAAGGT TCAAGAAATG GTAGATTCCA ATGAGGCTAA ACTAGTGGCT 
TTGGTGAACA AATTTATTGG TTATCTTAAA CAGAATACAT ACTGCTTTCC ACATAGTTTG 
AGGTGGATTG TGTCACAGAT GTACAAAACT CTTTCTTGTG TAGACAGATT GGAGGTTGGG 
GAAGTCAGAG CAATGTGTAC CGATCTTCTG CTGGCTTGCT TCATTTGTCC TGCAGTTGTC 
AATCCAGAGC AGTATGGGAT AATATCTGAT GCTCCTATTA ATGAGGTGGC AAGATTTAAC 
CTGATGCAGG TTGGCCGCCT TTTGCAGCAG CTAGCAATGA CTGGCACTGA AGAAGGAGAT 
CCCCGGACCA AAAATAGCCT TGGAAAATTT GACAAAGGCT GTGTCGCTGC TTTCCTTGAT 
GTTGTAATTG GGGGCCGTGC AGTGGAGACT CCGCCAATGT CCTCTGTTAA TCTTCTGGAA 
GGCTTGAGCA GAACTGTGGT GTATATAAGT TACAGTCAGC TTATTACTCT GGTAAATTTT 
ATGAAGAGTG TGATGTCTGG AGATCAACTG AAAGAAGATA GAATGGCTCT TGACAATTTG 
TTGGCAAACC TGCCCCAGGC TAAGCCAGGG AAGAGCAGCA GTCTGGACAT GACTCCCTAT 
AGCACTCCTC AGATGTCTCC AGCAACCACT CCAGCCAACA AGAAGAATCG ATTACCTATA 
GGACAACAGT TAGCAGCCAT CACTGCCTGG GATTCGTCAG CCACCAATCT TACTGCTCAT 
ATTCCTCTAG TAACCCCATT TGCAACTCGG AGCAGAAGCC GAAGTAATAT GTTAATGGAC 
CTACATATGG ATCATGAAGG ATCATCTCAA GAAACCATCC AGGAGGTACA GCCAGAAGAG 
GTGCTGGTCA TTTCCTTAGG GACAGGCCCT CAGCTCACTC CAGGGATGAT GTCAGAAAAT 
GAGGTTCTAA ACATGCAGCT TTCGGATGGA GGACAAGGAG ATGTCCCTGT TGATGAAAAC 
AAACTCCACG GTCCTTCCAA TCGCTCCAAT TCGGTGTCTT CTCTCGACCT GGAGGGAGAG 
TCTGTATCTG AACTTGGGGC AGGACCTTCT GGGAGTAACG GAGTTGAAGC ACTGCAGCTG 
TTAGAGCACG AGCAAGCTAC AACACAAGAT AATCTTGATG ATAAACTGAG GAAGTTTGAG 
ATTCGTGACA TGATGGGACT AACAGATGAC AGGGACATAT CCGAGACTGT GAGTGAGACC 
TGGAGCACTG ATGTCCTGGG AAGTGACTTC GACCCGAACG TTGATGAAGA TCGTTTGCAA 
GAGATTGCAG GTGCAGCAGC AGAGAACGTG TTAGGTAGTT TGCTGTGCCT TCCAGGTTCA 
GGGTCAGTTC TCCTTGACCC TTGCACTGGC TCTACCATAT CAGAGACCAC AAGTGAAGCT 
TGGAGTGTAG AGGTATTGCC AAGTGACTCA GAGGCTCCAG ACTTAAAGCA GGAAGAGCGC 
CTCCAAGAGC TGGAGAGCTG TTCTGGACTG GGCAGCACAT CTGATGATAC GGATGTCAGG 
GAGGTCAGTT CCCGCCCCAG CACACCAGGC CTCAGTGTTG TGTCCGGTAT AAGTGCTACA 
TCGGAGGATA TTCCCAATAA GATCGAAGAC CTGAGATCTG AGTGCAGCTC TGATTTTGGG 
GGCAAAGATT CTGTAACTAG TCCAGATATG GATGACATAG CCCATGGCGC TCATCAGCTG 
ACCTCTCCTC CCTCTCAGTC AGAGTCTCTG CTTGCCATGT TTGATCCACT GTCCTCACAT 
GAAGGGGCCT CTGCTGTGGT AAGGCCAAAG GTTCACTATG CCCGACCCTC GCATCCACCT 
CCGGATCCTC CAATCCTGGA AGGTGCAGTG GGAGGAAATG AGGCCAGGTT GCCAAACTTT 
GGCTCTCACG TTTTAACTGC AGCTGAAATG GAGGCGTTCA AGCAGAGACA CTCATACCCT 
GAGAGATTGG TTCGCAGCAG AAGCTCTGAT ATAGTGTCTT CTGTGCGGCG GCCCATGAGT 
GACCCCAGCT GGAACCGACG TCCAGGGAAT GAAGAACTTC CTCCAGCTGC AGCCACTGGT 
GCTACTTCTT TGGTGGCTGC ACCTCACTCG TCATCTTCGT CCCCGAGTAA GGACTCTTCT 
AGAGGAGAGA CTGAGGAACG CAAGGACAGT GACGATGAGA GGTCAGACAG GAGCAGGCCT 
TGGTGGAGGA AGCGCTTTGT TTCAGCTATG CCTAAAGCTC CTATACCATT TAGAAAGAAA 
GAAAAACAAG AAAAAGACAA AGATGATCTG GGTCCTGACA GATTCTCAAC ACTCACAGAT 
GAGCCCAGCC CTAGGCTCAG TGCTCAAGCT CAGGTTGCTG AAGATATTCT GGACAAGTAC 
CGGAATGCCA TTAAACGGAC CAGCCCCAGT GAAGGAGCAA TGGCAAATGA TGAGAGTGCA 
GAGGTCATGG GTGATGGTGA AAGTGCCCAC GACTCTCCTC GTGAGGAAGC ACTGCAGAAC 
ATCTCAGCCG ACGACCTCCC GGACTCCGCC AGCCAGGCAG CCCACCCTCA GGACTCTGCT 
TTCTCTTACA GAGATGTAAA AAAGAAGCTG AGGCTCGCAC TTTGCTCTGC AGACTCTGTG 
GCCTTCCCAG TGCTAACTCA TTCAACAAGG AATGGATTGC CAGACCACAC AGACCCAGAA 
GACAATGAAA TTGTATGCTT CTTAAAAGTT CAAATAGCTG AAGCAATTAA TTTACAAGAT 
AAGAGCTTAA TGGCTCAACT TCAAGAAACA ATGCGTTGTG TGTGCCGCTT TGACAATAGG 
ACTTGTAGGA AGTTGCTGGC ATCCATTGCT GAAGACTACA GAAAAAGAGC TCCGTATATT 
GCTTATCTTA CCCGCTGCCG ACAAGGACTG CAGACCACAC AGGCTCACTT GGAAAGGCTC 
TTGCAAAGAG TTTTGCGGGA CAAAGAGGTG GCCAATCGGT ACTTCACCAC TGTCTGTGTG 
CGGTTACTGC TTGAGAGCAA AGAAAAGAAA ATCAGGGAGT TCATTCAAGA CTTTCAGAAA 
CTTACTGCTG CTGATGATAA AACGGCTCAG GTGGAGGATT TTCTACAGTT CCTGTATGGT 
GTGATGGCTC AGGATGTCAT ATGGCAGAAT GCCAGTGAGG AGCAGCTGCA GGATGCCCAG 
CTGGCCATCG AGCGGAGTGT GATGAATCGG ATCTTCAAGC TTGCTTTCTA CCCTAACCAG 
GATGGGGACA TACTTCGTGA CCAGGTTCTG CATGAACATA TCCAGAGATT ATCTAAAGTA 
GTGACTGCAA ACCACAGAGC TCTGCAGATC CCTGAGGTTT ATCTCCGTGA GGCACCATGG 
CCATCTGCAC AGTCAGAAAT CAGGACAATA AGTGCTTACA AAACGCCCCG CGACAAAGTG 
CAGTGCATCC TGAGGATGTG CTCCACCATC ATGAACCTCT TGAGCCTGGC CAACGAGGAC 
TCTGTCCCCG GGGCAGATGA CTTTGTTCCT GTGTTGGTGT TTGTGTTAAT AAAGGCAAAC 
CCACCCTGCC TGCTGTCCAC TGTGCAGTAC ATCAGCAGCT TCTATGCCAG CTGTCTGTCT 
GGAGAGGAGT CCTACTGGTG GATGCAGTTC ACAGCAGCCG TGGAGTTTAT TAAAACCATC 
GATGACAGAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030107814.1
CDS	328..4722
Translation	MVKLDIHTLAHHLKQERLYVSSEKQLIQRLNADVLKTAEKLYRTAWIAKQQRINLDRLIITSAEASPAECCQHAKILEDTQFVDGYKQLGFQETAYGEFLSRLRENPRLIASSLVAGEKLNQENTQSVIYTVFTSLYGNCIMQEDESYLLQVLRYLIEFELKESDNPRRLLRRGTCAFSILFKLFSEGLFSAKLFLTATLHEPIMQLLVEDEDHLETDPNKLIERFSPAQQEKLFGEKGSDRFRQKVQEMVDSNEAKLVALVNKFIGYLKQNTYCFPHSLRWIVSQMYKTLSCVDRLEVGEVRAMCTDLLLACFICPAVVNPEQYGIISDAPINEVARFNLMQVGRLLQQLAMTGTEEGDPRTKNSLGKFDKGCVAAFLDVVIGGRAVETPPMSSVNLLEGLSRTVVYISYSQLITLVNFMKSVMSGDQLKEDRMALDNLLANLPQAKPGKSSSLDMTPYSTPQMSPATTPANKKNRLPIGQQLAAITAWDSSATNLTAHIPLVTPFATRSRSRSNMLMDLHMDHEGSSQETIQEVQPEEVLVISLGTGPQLTPGMMSENEVLNMQLSDGGQGDVPVDENKLHGPSNRSNSVSSLDLEGESVSELGAGPSGSNGVEALQLLEHEQATTQDNLDDKLRKFEIRDMMGLTDDRDISETVSETWSTDVLGSDFDPNVDEDRLQEIAGAAAENVLGSLLCLPGSGSVLLDPCTGSTISETTSEAWSVEVLPSDSEAPDLKQEERLQELESCSGLGSTSDDTDVREVSSRPSTPGLSVVSGISATSEDIPNKIEDLRSECSSDFGGKDSVTSPDMDDIAHGAHQLTSPPSQSESLLAMFDPLSSHEGASAVVRPKVHYARPSHPPPDPPILEGAVGGNEARLPNFGSHVLTAAEMEAFKQRHSYPERLVRSRSSDIVSSVRRPMSDPSWNRRPGNEELPPAAATGATSLVAAPHSSSSSPSKDSSRGETEERKDSDDERSDRSRPWWRKRFVSAMPKAPIPFRKKEKQEKDKDDLGPDRFSTLTDEPSPRLSAQAQVAEDILDKYRNAIKRTSPSEGAMANDESAEVMGDGESAHDSPREEALQNISADDLPDSASQAAHPQDSAFSYRDVKKKLRLALCSADSVAFPVLTHSTRNGLPDHTDPEDNEIVCFLKVQIAEAINLQDKSLMAQLQETMRCVCRFDNRTCRKLLASIAEDYRKRAPYIAYLTRCRQGLQTTQAHLERLLQRVLRDKEVANRYFTTVCVRLLLESKEKKIREFIQDFQKLTAADDKTAQVEDFLQFLYGVMAQDVIWQNASEEQLQDAQLAIERSVMNRIFKLAFYPNQDGDILRDQVLHEHIQRLSKVVTANHRALQIPEVYLREAPWPSAQSEIRTISAYKTPRDKVQCILRMCSTIMNLLSLANEDSVPGADDFVPVLVFVLIKANPPCLLSTVQYISSFYASCLSGEESYWWMQFTAAVEFIKTIDDRK

Target ORF information:

RefSeq Version	XM_030251954.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus GTPase activating protein and VPS9 domains 1 (Gapvd1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030251954.1

ORF Insert Sequence:

ATGGTGAAGC TAGATATTCA CACATTGGCC CACCACCTCA AGCAGGAACG CTTATATGTG 
AGCTCTGAGA AACAGCTCAT TCAGAGGCTA AATGCCGATG TACTGAAGAC AGCTGAAAAG 
CTGTATCGAA CAGCGTGGAT TGCAAAGCAA CAGAGGATTA ATTTGGATCG TCTTATTATA 
ACCAGTGCTG AAGCTTCCCC TGCTGAGTGT TGCCAGCATG CCAAGATTTT GGAAGATACA 
CAGTTTGTTG ATGGATATAA GCAACTGGGA TTTCAGGAAA CAGCTTATGG AGAATTCCTG 
AGTCGGTTAA GAGAAAATCC ACGACTTATT GCCTCCTCTT TGGTTGCTGG AGAAAAACTC 
AATCAGGAGA ACACACAAAG TGTTATCTAC ACTGTTTTTA CCTCCTTGTA TGGCAACTGT 
ATTATGCAAG AAGATGAAAG CTACCTCCTT CAGGTTTTAC GATACTTGAT TGAATTTGAA 
CTTAAAGAAA GTGACAACCC CAGGCGCCTT TTGAGGAGAG GAACTTGTGC CTTCAGCATC 
TTATTTAAAC TTTTTTCTGA AGGACTGTTT TCTGCCAAAC TTTTCCTTAC GGCTACTTTG 
CATGAGCCAA TCATGCAGCT CCTCGTAGAG GATGAAGACC ACCTGGAAAC AGATCCAAAC 
AAGCTGATAG AGAGATTCTC TCCAGCTCAG CAAGAAAAAC TCTTTGGAGA GAAAGGCTCA 
GATAGATTTA GGCAAAAGGT TCAAGAAATG GTAGATTCCA ATGAGGCTAA ACTAGTGGCT 
TTGGTGAACA AATTTATTGG TTATCTTAAA CAGAATACAT ACTGCTTTCC ACATAGTTTG 
AGGTGGATTG TGTCACAGAT GTACAAAACT CTTTCTTGTG TAGACAGATT GGAGGTTGGG 
GAAGTCAGAG CAATGTGTAC CGATCTTCTG CTGGCTTGCT TCATTTGTCC TGCAGTTGTC 
AATCCAGAGC AGTATGGGAT AATATCTGAT GCTCCTATTA ATGAGGTGGC AAGATTTAAC 
CTGATGCAGG TTGGCCGCCT TTTGCAGCAG CTAGCAATGA CTGGCACTGA AGAAGGAGAT 
CCCCGGACCA AAAATAGCCT TGGAAAATTT GACAAAGGCT GTGTCGCTGC TTTCCTTGAT 
GTTGTAATTG GGGGCCGTGC AGTGGAGACT CCGCCAATGT CCTCTGTTAA TCTTCTGGAA 
GGCTTGAGCA GAACTGTGGT GTATATAAGT TACAGTCAGC TTATTACTCT GGTAAATTTT 
ATGAAGAGTG TGATGTCTGG AGATCAACTG AAAGAAGATA GAATGGCTCT TGACAATTTG 
TTGGCAAACC TGCCCCAGGC TAAGCCAGGG AAGAGCAGCA GTCTGGACAT GACTCCCTAT 
AGCACTCCTC AGATGTCTCC AGCAACCACT CCAGCCAACA AGAAGAATCG ATTACCTATA 
GGACAACAGT TAGCAGCCAT CACTGCCTGG GATTCGTCAG CCACCAATCT TACTGCTCAT 
ATTCCTCTAG TAACCCCATT TGCAACTCGG AGCAGAAGCC GAAGTAATAT GTTAATGGAC 
CTACATATGG ATCATGAAGG ATCATCTCAA GAAACCATCC AGGAGGTACA GCCAGAAGAG 
GTGCTGGTCA TTTCCTTAGG GACAGGCCCT CAGCTCACTC CAGGGATGAT GTCAGAAAAT 
GAGGTTCTAA ACATGCAGCT TTCGGATGGA GGACAAGGAG ATGTCCCTGT TGATGAAAAC 
AAACTCCACG GTCCTTCCAA TCGCTCCAAT TCGGTGTCTT CTCTCGACCT GGAGGGAGAG 
TCTGTATCTG AACTTGGGGC AGGACCTTCT GGGAGTAACG GAGTTGAAGC ACTGCAGCTG 
TTAGAGCACG AGCAAGCTAC AACACAAGAT AATCTTGATG ATAAACTGAG GAAGTTTGAG 
ATTCGTGACA TGATGGGACT AACAGATGAC AGGGACATAT CCGAGACTGT GAGTGAGACC 
TGGAGCACTG ATGTCCTGGG AAGTGACTTC GACCCGAACG TTGATGAAGA TCGTTTGCAA 
GAGATTGCAG GTGCAGCAGC AGAGAACGTG TTAGGTAGTT TGCTGTGCCT TCCAGGTTCA 
GGGTCAGTTC TCCTTGACCC TTGCACTGGC TCTACCATAT CAGAGACCAC AAGTGAAGCT 
TGGAGTGTAG AGGTATTGCC AAGTGACTCA GAGGCTCCAG ACTTAAAGCA GGAAGAGCGC 
CTCCAAGAGC TGGAGAGCTG TTCTGGACTG GGCAGCACAT CTGATGATAC GGATGTCAGG 
GAGGTCAGTT CCCGCCCCAG CACACCAGGC CTCAGTGTTG TGTCCGGTAT AAGTGCTACA 
TCGGAGGATA TTCCCAATAA GATCGAAGAC CTGAGATCTG AGTGCAGCTC TGATTTTGGG 
GGCAAAGATT CTGTAACTAG TCCAGATATG GATGACATAG CCCATGGCGC TCATCAGCTG 
ACCTCTCCTC CCTCTCAGTC AGAGTCTCTG CTTGCCATGT TTGATCCACT GTCCTCACAT 
GAAGGGGCCT CTGCTGTGGT AAGGCCAAAG GTTCACTATG CCCGACCCTC GCATCCACCT 
CCGGATCCTC CAATCCTGGA AGGTGCAGTG GGAGGAAATG AGGCCAGGTT GCCAAACTTT 
GGCTCTCACG TTTTAACTGC AGCTGAAATG GAGGCGTTCA AGCAGAGACA CTCATACCCT 
GAGAGATTGG TTCGCAGCAG AAGCTCTGAT ATAGTGTCTT CTGTGCGGCG GCCCATGAGT 
GACCCCAGCT GGAACCGACG TCCAGGGAAT GAAGAACTTC CTCCAGCTGC AGCCACTGGT 
GCTACTTCTT TGGTGGCTGC ACCTCACTCG TCATCTTCGT CCCCGAGTAA GGACTCTTCT 
AGAGGAGAGA CTGAGGAACG CAAGGACAGT GACGATGAGA GGTCAGACAG GAGCAGGCCT 
TGGTGGAGGA AGCGCTTTGT TTCAGCTATG CCTAAAGCTC CTATACCATT TAGAAAGAAA 
GAAAAACAAG AAAAAGACAA AGATGATCTG GGTCCTGACA GATTCTCAAC ACTCACAGAT 
GAGCCCAGCC CTAGGCTCAG TGCTCAAGCT CAGGTTGCTG AAGATATTCT GGACAAGTAC 
CGGAATGCCA TTAAACGGAC CAGCCCCAGT GAAGGAGCAA TGGCAAATGA TGAGAGTGCA 
GAGGTCATGG GTGATGGTGA AAGTGCCCAC GACTCTCCTC GTGAGGAAGC ACTGCAGAAC 
ATCTCAGCCG ACGACCTCCC GGACTCCGCC AGCCAGGCAG CCCACCCTCA GGACTCTGCT 
TTCTCTTACA GAGATGTAAA AAAGAAGCTG AGGCTCGCAC TTTGCTCTGC AGACTCTGTG 
GCCTTCCCAG TGCTAACTCA TTCAACAAGG AATGGATTGC CAGACCACAC AGACCCAGAA 
GACAATGAAA TTGTATGCTT CTTAAAAGTT CAAATAGCTG AAGCAATTAA TTTACAAGAT 
AAGAGCTTAA TGGCTCAACT TCAAGAAACA ATGCGTTGTG TGTGCCGCTT TGACAATAGG 
ACTTGTAGGA AGTTGCTGGC ATCCATTGCT GAAGACTACA GAAAAAGAGC TCCGTATATT 
GCTTATCTTA CCCGCTGCCG ACAAGGACTG CAGACCACAC AGGCTCACTT GGAAAGGCTC 
TTGCAAAGAG TTTTGCGGGA CAAAGAGGTG GCCAATCGGT ACTTCACCAC TGTCTGTGTG 
CGGTTACTGC TTGAGAGCAA AGAAAAGAAA ATCAGGGAGT TCATTCAAGA CTTTCAGAAA 
CTTACTGCTG CTGATGATAA AACGGCTCAG GTGGAGGATT TTCTACAGTT CCTGTATGGT 
GTGATGGCTC AGGATGTCAT ATGGCAGAAT GCCAGTGAGG AGCAGCTGCA GGATGCCCAG 
CTGGCCATCG AGCGGAGTGT GATGAATCGG ATCTTCAAGC TTGCTTTCTA CCCTAACCAG 
GATGGGGACA TACTTCGTGA CCAGGTTCTG CATGAACATA TCCAGAGATT ATCTAAAGTA 
GTGACTGCAA ACCACAGAGC TCTGCAGATC CCTGAGGTTT ATCTCCGTGA GGCACCATGG 
CCATCTGCAC AGTCAGAAAT CAGGACAATA AGTGCTTACA AAACGCCCCG CGACAAAGTG 
CAGTGCATCC TGAGGATGTG CTCCACCATC ATGAACCTCT TGAGCCTGGC CAACGAGGAC 
TCTGTCCCCG GGGCAGATGA CTTTGTTCCT GTGTTGGTGT TTGTGTTAAT AAAGGCAAAC 
CCACCCTGCC TGCTGTCCAC TGTGCAGTAC ATCAGCAGCT TCTATGCCAG CTGTCTGTCT 
GGAGAGGAGT CCTACTGGTG GATGCAGTTC ACAGCAGCCG TGGAGTTTAT TAAAACCATC 
GATGACAGAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAPVD1 ( XM_030251955.1 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_030107815.1 Mus musculus GTPase activating protein and VPS9 domains 1 (Gapvd1), transcript variant X4, mRNA.

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CloneID	OMu69991
Clone ID Related Accession (Same CDS sequence)	XM_011239149.2 , XM_030251955.1
Accession Version	XM_030251955.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4377bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	GTPase-activating protein and VPS9 domain-containing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGC TAGATATTCA CACATTGGCC CACCACCTCA AGCAGGAACG CTTATATGTG 
AGCTCTGAGA AACAGCTCAT TCAGAGGCTA AATGCCGATG TACTGAAGAC AGCTGAAAAG 
CTGTATCGAA CAGCGTGGAT TGCAAAGCAA CAGAGGATTA ATTTGGATCG TCTTATTATA 
ACCAGTGCTG AAGCTTCCCC TGCTGAGTGT TGCCAGCATG CCAAGATTTT GGAAGATACA 
CAGTTTGTTG ATGGATATAA GCAACTGGGA TTTCAGGAAA CAGCTTATGG AGAATTCCTG 
AGTCGGTTAA GAGAAAATCC ACGACTTATT GCCTCCTCTT TGGTTGCTGG AGAAAAACTC 
AATCAGGAGA ACACACAAAG TGTTATCTAC ACTGTTTTTA CCTCCTTGTA TGGCAACTGT 
ATTATGCAAG AAGATGAAAG CTACCTCCTT CAGGTTTTAC GATACTTGAT TGAATTTGAA 
CTTAAAGAAA GTGACAACCC CAGGCGCCTT TTGAGGAGAG GAACTTGTGC CTTCAGCATC 
TTATTTAAAC TTTTTTCTGA AGGACTGTTT TCTGCCAAAC TTTTCCTTAC GGCTACTTTG 
CATGAGCCAA TCATGCAGCT CCTCGTAGAG GATGAAGACC ACCTGGAAAC AGATCCAAAC 
AAGCTGATAG AGAGATTCTC TCCAGCTCAG CAAGAAAAAC TCTTTGGAGA GAAAGGCTCA 
GATAGATTTA GGCAAAAGGT TCAAGAAATG GTAGATTCCA ATGAGGCTAA ACTAGTGGCT 
TTGGTGAACA AATTTATTGG TTATCTTAAA CAGAATACAT ACTGCTTTCC ACATAGTTTG 
AGGTGGATTG TGTCACAGAT GTACAAAACT CTTTCTTGTG TAGACAGATT GGAGGTTGGG 
GAAGTCAGAG CAATGTGTAC CGATCTTCTG CTGGCTTGCT TCATTTGTCC TGCAGTTGTC 
AATCCAGAGC AGTATGGGAT AATATCTGAT GCTCCTATTA ATGAGGTGGC AAGATTTAAC 
CTGATGCAGG TTGGCCGCCT TTTGCAGCAG CTAGCAATGA CTGGCACTGA AGAAGGAGAT 
CCCCGGACCA AAAATAGCCT TGGAAAATTT GACAAAGGCT GTGTCGCTGC TTTCCTTGAT 
GTTGTAATTG GGGGCCGTGC AGTGGAGACT CCGCCAATGT CCTCTGTTAA TCTTCTGGAA 
GGCTTGAGCA GAACTGTGGT GTATATAAGT TACAGTCAGC TTATTACTCT GGTAAATTTT 
ATGAAGAGTG TGATGTCTGG AGATCAACTG AAAGAAGATA GAATGGCTCT TGACAATTTG 
TTGGCAAACC TGCCCCAGGC TAAGCCAGGG AAGAGCAGCA GTCTGGACAT GACTCCCTAT 
AGCACTCCTC AGATGTCTCC AGCAACCACT CCAGCCAACA AGAAGAATCG ATTACCTATA 
GGACAACAGT TAGCAGCCAT CACTGCCTGG GATTCGTCAG CCACCAATCT TACTGCTCAT 
ATTCCTCTAG TAACCCCATT TGCAACTCGG AGCAGAAGCC GAAGTAATAT GTTAATGGAC 
CTACATATGG ATCATGAAGG ATCATCTCAA GAAACCATCC AGGAGGTACA GCCAGAAGAG 
GTGCTGGTCA TTTCCTTAGG GACAGGCCCT CAGCTCACTC CAGGGATGAT GTCAGAAAAT 
GAGGTTCTAA ACATGCAGCT TTCGGATGGA GGACAAGGAG ATGTCCCTGT TGATGAAAAC 
AAACTCCACG GTAAACCTGA TAAAACCTTG CGCTTTTCCC TCTGCAGTGA TAATCTGGAA 
GGCATATCTG AAGGTCCTTC CAATCGCTCC AATTCGGTGT CTTCTCTCGA CCTGGAGGGA 
GAGTCTGTAT CTGAACTTGG GGCAGGACCT TCTGGGAGTA ACGGAGTTGA AGCACTGCAG 
CTGTTAGAGC ACGAGCAAGC TACAACACAA GATAATCTTG ATGATAAACT GAGGAAGTTT 
GAGATTCGTG ACATGATGGG ACTAACAGAT GACAGGGACA TATCCGAGAC TGTGAGTGAG 
ACCTGGAGCA CTGATGTCCT GGGAAGTGAC TTCGACCCGA ACGTTGATGA AGATCGTTTG 
CAAGAGATTG CAGGTGCAGC AGCAGAGAAC GTGTTAGGTA GTTTGCTGTG CCTTCCAGGT 
TCAGGGTCAG TTCTCCTTGA CCCTTGCACT GGCTCTACCA TATCAGAGAC CACAAGTGAA 
GCTTGGAGTG TAGAGGTATT GCCAAGTGAC TCAGAGGCTC CAGACTTAAA GCAGGAAGAG 
CGCCTCCAAG AGCTGGAGAG CTGTTCTGGA CTGGGCAGCA CATCTGATGA TACGGATGTC 
AGGGAGGTCA GTTCCCGCCC CAGCACACCA GGCCTCAGTG TTGTGTCCGG TATAAGTGCT 
ACATCGGAGG ATATTCCCAA TAAGATCGAA GACCTGAGAT CTGAGTGCAG CTCTGATTTT 
GGGGGCAAAG ATTCTGTAAC TAGTCCAGAT ATGGATGACA TAGCCCATGG CGCTCATCAG 
CTGACCTCTC CTCCCTCTCA GTCAGAGTCT CTGCTTGCCA TGTTTGATCC ACTGTCCTCA 
CATGAAGGGG CCTCTGCTGT GGTAAGGCCA AAGGTTCACT ATGCCCGACC CTCGCATCCA 
CCTCCGGATC CTCCAATCCT GGAAGGTGCA GTGGGAGGAA ATGAGGCCAG GTTGCCAAAC 
TTTGGCTCTC ACGTTTTAAC TGCAGCTGAA ATGGAGGCGT TCAAGCAGAG ACACTCATAC 
CCTGAGAGAT TGGTTCGCAG CAGAAGCTCT GATATAGTGT CTTCTGTGCG GCGGCCCATG 
AGTGACCCCA GCTGGAACCG ACGTCCAGGG AATGAAGAAC TTCCTCCAGC TGCAGCCACT 
GGTGCTACTT CTTTGGTGGC TGCACCTCAC TCGTCATCTT CGTCCCCGAG TAAGGACTCT 
TCTAGAGGAG AGACTGAGGA ACGCAAGGAC AGTGACGATG AGAGGTCAGA CAGGAGCAGG 
CCTTGGTGGA GGAAGCGCTT TGTTTCAGCT ATGCCTAAAG ATGAGCCCAG CCCTAGGCTC 
AGTGCTCAAG CTCAGGTTGC TGAAGATATT CTGGACAAGT ACCGGAATGC CATTAAACGG 
ACCAGCCCCA GTGAAGGAGC AATGGCAAAT GATGAGAGTG CAGAGGTCAT GGGTGATGGT 
GAAAGTGCCC ACGACTCTCC TCGTGAGGAA GCACTGCAGA ACATCTCAGC CGACGACCTC 
CCGGACTCCG CCAGCCAGGC AGCCCACCCT CAGGACTCTG CTTTCTCTTA CAGAGATGTA 
AAAAAGAAGC TGAGGCTCGC ACTTTGCTCT GCAGACTCTG TGGCCTTCCC AGTGCTAACT 
CATTCAACAA GGAATGGATT GCCAGACCAC ACAGACCCAG AAGACAATGA AATTGTATGC 
TTCTTAAAAG TTCAAATAGC TGAAGCAATT AATTTACAAG ATAAGAGCTT AATGGCTCAA 
CTTCAAGAAA CAATGCGTTG TGTGTGCCGC TTTGACAATA GGACTTGTAG GAAGTTGCTG 
GCATCCATTG CTGAAGACTA CAGAAAAAGA GCTCCGTATA TTGCTTATCT TACCCGCTGC 
CGACAAGGAC TGCAGACCAC ACAGGCTCAC TTGGAAAGGC TCTTGCAAAG AGTTTTGCGG 
GACAAAGAGG TGGCCAATCG GTACTTCACC ACTGTCTGTG TGCGGTTACT GCTTGAGAGC 
AAAGAAAAGA AAATCAGGGA GTTCATTCAA GACTTTCAGA AACTTACTGC TGCTGATGAT 
AAAACGGCTC AGGTGGAGGA TTTTCTACAG TTCCTGTATG GTGTGATGGC TCAGGATGTC 
ATATGGCAGA ATGCCAGTGA GGAGCAGCTG CAGGATGCCC AGCTGGCCAT CGAGCGGAGT 
GTGATGAATC GGATCTTCAA GCTTGCTTTC TACCCTAACC AGGATGGGGA CATACTTCGT 
GACCAGGTTC TGCATGAACA TATCCAGAGA TTATCTAAAG TAGTGACTGC AAACCACAGA 
GCTCTGCAGA TCCCTGAGGT TTATCTCCGT GAGGCACCAT GGCCATCTGC ACAGTCAGAA 
ATCAGGACAA TAAGTGCTTA CAAAACGCCC CGCGACAAAG TGCAGTGCAT CCTGAGGATG 
TGCTCCACCA TCATGAACCT CTTGAGCCTG GCCAACGAGG ACTCTGTCCC CGGGGCAGAT 
GACTTTGTTC CTGTGTTGGT GTTTGTGTTA ATAAAGGCAA ACCCACCCTG CCTGCTGTCC 
ACTGTGCAGT ACATCAGCAG CTTCTATGCC AGCTGTCTGT CTGGAGAGGA GTCCTACTGG 
TGGATGCAGT TCACAGCAGC CGTGGAGTTT ATTAAAACCA TCGATGACAG AAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030107815.1
CDS	328..4704
Translation	MVKLDIHTLAHHLKQERLYVSSEKQLIQRLNADVLKTAEKLYRTAWIAKQQRINLDRLIITSAEASPAECCQHAKILEDTQFVDGYKQLGFQETAYGEFLSRLRENPRLIASSLVAGEKLNQENTQSVIYTVFTSLYGNCIMQEDESYLLQVLRYLIEFELKESDNPRRLLRRGTCAFSILFKLFSEGLFSAKLFLTATLHEPIMQLLVEDEDHLETDPNKLIERFSPAQQEKLFGEKGSDRFRQKVQEMVDSNEAKLVALVNKFIGYLKQNTYCFPHSLRWIVSQMYKTLSCVDRLEVGEVRAMCTDLLLACFICPAVVNPEQYGIISDAPINEVARFNLMQVGRLLQQLAMTGTEEGDPRTKNSLGKFDKGCVAAFLDVVIGGRAVETPPMSSVNLLEGLSRTVVYISYSQLITLVNFMKSVMSGDQLKEDRMALDNLLANLPQAKPGKSSSLDMTPYSTPQMSPATTPANKKNRLPIGQQLAAITAWDSSATNLTAHIPLVTPFATRSRSRSNMLMDLHMDHEGSSQETIQEVQPEEVLVISLGTGPQLTPGMMSENEVLNMQLSDGGQGDVPVDENKLHGKPDKTLRFSLCSDNLEGISEGPSNRSNSVSSLDLEGESVSELGAGPSGSNGVEALQLLEHEQATTQDNLDDKLRKFEIRDMMGLTDDRDISETVSETWSTDVLGSDFDPNVDEDRLQEIAGAAAENVLGSLLCLPGSGSVLLDPCTGSTISETTSEAWSVEVLPSDSEAPDLKQEERLQELESCSGLGSTSDDTDVREVSSRPSTPGLSVVSGISATSEDIPNKIEDLRSECSSDFGGKDSVTSPDMDDIAHGAHQLTSPPSQSESLLAMFDPLSSHEGASAVVRPKVHYARPSHPPPDPPILEGAVGGNEARLPNFGSHVLTAAEMEAFKQRHSYPERLVRSRSSDIVSSVRRPMSDPSWNRRPGNEELPPAAATGATSLVAAPHSSSSSPSKDSSRGETEERKDSDDERSDRSRPWWRKRFVSAMPKDEPSPRLSAQAQVAEDILDKYRNAIKRTSPSEGAMANDESAEVMGDGESAHDSPREEALQNISADDLPDSASQAAHPQDSAFSYRDVKKKLRLALCSADSVAFPVLTHSTRNGLPDHTDPEDNEIVCFLKVQIAEAINLQDKSLMAQLQETMRCVCRFDNRTCRKLLASIAEDYRKRAPYIAYLTRCRQGLQTTQAHLERLLQRVLRDKEVANRYFTTVCVRLLLESKEKKIREFIQDFQKLTAADDKTAQVEDFLQFLYGVMAQDVIWQNASEEQLQDAQLAIERSVMNRIFKLAFYPNQDGDILRDQVLHEHIQRLSKVVTANHRALQIPEVYLREAPWPSAQSEIRTISAYKTPRDKVQCILRMCSTIMNLLSLANEDSVPGADDFVPVLVFVLIKANPPCLLSTVQYISSFYASCLSGEESYWWMQFTAAVEFIKTIDDRK

Target ORF information:

RefSeq Version	XM_030251955.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus GTPase activating protein and VPS9 domains 1 (Gapvd1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030251955.1

ORF Insert Sequence:

ATGGTGAAGC TAGATATTCA CACATTGGCC CACCACCTCA AGCAGGAACG CTTATATGTG 
AGCTCTGAGA AACAGCTCAT TCAGAGGCTA AATGCCGATG TACTGAAGAC AGCTGAAAAG 
CTGTATCGAA CAGCGTGGAT TGCAAAGCAA CAGAGGATTA ATTTGGATCG TCTTATTATA 
ACCAGTGCTG AAGCTTCCCC TGCTGAGTGT TGCCAGCATG CCAAGATTTT GGAAGATACA 
CAGTTTGTTG ATGGATATAA GCAACTGGGA TTTCAGGAAA CAGCTTATGG AGAATTCCTG 
AGTCGGTTAA GAGAAAATCC ACGACTTATT GCCTCCTCTT TGGTTGCTGG AGAAAAACTC 
AATCAGGAGA ACACACAAAG TGTTATCTAC ACTGTTTTTA CCTCCTTGTA TGGCAACTGT 
ATTATGCAAG AAGATGAAAG CTACCTCCTT CAGGTTTTAC GATACTTGAT TGAATTTGAA 
CTTAAAGAAA GTGACAACCC CAGGCGCCTT TTGAGGAGAG GAACTTGTGC CTTCAGCATC 
TTATTTAAAC TTTTTTCTGA AGGACTGTTT TCTGCCAAAC TTTTCCTTAC GGCTACTTTG 
CATGAGCCAA TCATGCAGCT CCTCGTAGAG GATGAAGACC ACCTGGAAAC AGATCCAAAC 
AAGCTGATAG AGAGATTCTC TCCAGCTCAG CAAGAAAAAC TCTTTGGAGA GAAAGGCTCA 
GATAGATTTA GGCAAAAGGT TCAAGAAATG GTAGATTCCA ATGAGGCTAA ACTAGTGGCT 
TTGGTGAACA AATTTATTGG TTATCTTAAA CAGAATACAT ACTGCTTTCC ACATAGTTTG 
AGGTGGATTG TGTCACAGAT GTACAAAACT CTTTCTTGTG TAGACAGATT GGAGGTTGGG 
GAAGTCAGAG CAATGTGTAC CGATCTTCTG CTGGCTTGCT TCATTTGTCC TGCAGTTGTC 
AATCCAGAGC AGTATGGGAT AATATCTGAT GCTCCTATTA ATGAGGTGGC AAGATTTAAC 
CTGATGCAGG TTGGCCGCCT TTTGCAGCAG CTAGCAATGA CTGGCACTGA AGAAGGAGAT 
CCCCGGACCA AAAATAGCCT TGGAAAATTT GACAAAGGCT GTGTCGCTGC TTTCCTTGAT 
GTTGTAATTG GGGGCCGTGC AGTGGAGACT CCGCCAATGT CCTCTGTTAA TCTTCTGGAA 
GGCTTGAGCA GAACTGTGGT GTATATAAGT TACAGTCAGC TTATTACTCT GGTAAATTTT 
ATGAAGAGTG TGATGTCTGG AGATCAACTG AAAGAAGATA GAATGGCTCT TGACAATTTG 
TTGGCAAACC TGCCCCAGGC TAAGCCAGGG AAGAGCAGCA GTCTGGACAT GACTCCCTAT 
AGCACTCCTC AGATGTCTCC AGCAACCACT CCAGCCAACA AGAAGAATCG ATTACCTATA 
GGACAACAGT TAGCAGCCAT CACTGCCTGG GATTCGTCAG CCACCAATCT TACTGCTCAT 
ATTCCTCTAG TAACCCCATT TGCAACTCGG AGCAGAAGCC GAAGTAATAT GTTAATGGAC 
CTACATATGG ATCATGAAGG ATCATCTCAA GAAACCATCC AGGAGGTACA GCCAGAAGAG 
GTGCTGGTCA TTTCCTTAGG GACAGGCCCT CAGCTCACTC CAGGGATGAT GTCAGAAAAT 
GAGGTTCTAA ACATGCAGCT TTCGGATGGA GGACAAGGAG ATGTCCCTGT TGATGAAAAC 
AAACTCCACG GTAAACCTGA TAAAACCTTG CGCTTTTCCC TCTGCAGTGA TAATCTGGAA 
GGCATATCTG AAGGTCCTTC CAATCGCTCC AATTCGGTGT CTTCTCTCGA CCTGGAGGGA 
GAGTCTGTAT CTGAACTTGG GGCAGGACCT TCTGGGAGTA ACGGAGTTGA AGCACTGCAG 
CTGTTAGAGC ACGAGCAAGC TACAACACAA GATAATCTTG ATGATAAACT GAGGAAGTTT 
GAGATTCGTG ACATGATGGG ACTAACAGAT GACAGGGACA TATCCGAGAC TGTGAGTGAG 
ACCTGGAGCA CTGATGTCCT GGGAAGTGAC TTCGACCCGA ACGTTGATGA AGATCGTTTG 
CAAGAGATTG CAGGTGCAGC AGCAGAGAAC GTGTTAGGTA GTTTGCTGTG CCTTCCAGGT 
TCAGGGTCAG TTCTCCTTGA CCCTTGCACT GGCTCTACCA TATCAGAGAC CACAAGTGAA 
GCTTGGAGTG TAGAGGTATT GCCAAGTGAC TCAGAGGCTC CAGACTTAAA GCAGGAAGAG 
CGCCTCCAAG AGCTGGAGAG CTGTTCTGGA CTGGGCAGCA CATCTGATGA TACGGATGTC 
AGGGAGGTCA GTTCCCGCCC CAGCACACCA GGCCTCAGTG TTGTGTCCGG TATAAGTGCT 
ACATCGGAGG ATATTCCCAA TAAGATCGAA GACCTGAGAT CTGAGTGCAG CTCTGATTTT 
GGGGGCAAAG ATTCTGTAAC TAGTCCAGAT ATGGATGACA TAGCCCATGG CGCTCATCAG 
CTGACCTCTC CTCCCTCTCA GTCAGAGTCT CTGCTTGCCA TGTTTGATCC ACTGTCCTCA 
CATGAAGGGG CCTCTGCTGT GGTAAGGCCA AAGGTTCACT ATGCCCGACC CTCGCATCCA 
CCTCCGGATC CTCCAATCCT GGAAGGTGCA GTGGGAGGAA ATGAGGCCAG GTTGCCAAAC 
TTTGGCTCTC ACGTTTTAAC TGCAGCTGAA ATGGAGGCGT TCAAGCAGAG ACACTCATAC 
CCTGAGAGAT TGGTTCGCAG CAGAAGCTCT GATATAGTGT CTTCTGTGCG GCGGCCCATG 
AGTGACCCCA GCTGGAACCG ACGTCCAGGG AATGAAGAAC TTCCTCCAGC TGCAGCCACT 
GGTGCTACTT CTTTGGTGGC TGCACCTCAC TCGTCATCTT CGTCCCCGAG TAAGGACTCT 
TCTAGAGGAG AGACTGAGGA ACGCAAGGAC AGTGACGATG AGAGGTCAGA CAGGAGCAGG 
CCTTGGTGGA GGAAGCGCTT TGTTTCAGCT ATGCCTAAAG ATGAGCCCAG CCCTAGGCTC 
AGTGCTCAAG CTCAGGTTGC TGAAGATATT CTGGACAAGT ACCGGAATGC CATTAAACGG 
ACCAGCCCCA GTGAAGGAGC AATGGCAAAT GATGAGAGTG CAGAGGTCAT GGGTGATGGT 
GAAAGTGCCC ACGACTCTCC TCGTGAGGAA GCACTGCAGA ACATCTCAGC CGACGACCTC 
CCGGACTCCG CCAGCCAGGC AGCCCACCCT CAGGACTCTG CTTTCTCTTA CAGAGATGTA 
AAAAAGAAGC TGAGGCTCGC ACTTTGCTCT GCAGACTCTG TGGCCTTCCC AGTGCTAACT 
CATTCAACAA GGAATGGATT GCCAGACCAC ACAGACCCAG AAGACAATGA AATTGTATGC 
TTCTTAAAAG TTCAAATAGC TGAAGCAATT AATTTACAAG ATAAGAGCTT AATGGCTCAA 
CTTCAAGAAA CAATGCGTTG TGTGTGCCGC TTTGACAATA GGACTTGTAG GAAGTTGCTG 
GCATCCATTG CTGAAGACTA CAGAAAAAGA GCTCCGTATA TTGCTTATCT TACCCGCTGC 
CGACAAGGAC TGCAGACCAC ACAGGCTCAC TTGGAAAGGC TCTTGCAAAG AGTTTTGCGG 
GACAAAGAGG TGGCCAATCG GTACTTCACC ACTGTCTGTG TGCGGTTACT GCTTGAGAGC 
AAAGAAAAGA AAATCAGGGA GTTCATTCAA GACTTTCAGA AACTTACTGC TGCTGATGAT 
AAAACGGCTC AGGTGGAGGA TTTTCTACAG TTCCTGTATG GTGTGATGGC TCAGGATGTC 
ATATGGCAGA ATGCCAGTGA GGAGCAGCTG CAGGATGCCC AGCTGGCCAT CGAGCGGAGT 
GTGATGAATC GGATCTTCAA GCTTGCTTTC TACCCTAACC AGGATGGGGA CATACTTCGT 
GACCAGGTTC TGCATGAACA TATCCAGAGA TTATCTAAAG TAGTGACTGC AAACCACAGA 
GCTCTGCAGA TCCCTGAGGT TTATCTCCGT GAGGCACCAT GGCCATCTGC ACAGTCAGAA 
ATCAGGACAA TAAGTGCTTA CAAAACGCCC CGCGACAAAG TGCAGTGCAT CCTGAGGATG 
TGCTCCACCA TCATGAACCT CTTGAGCCTG GCCAACGAGG ACTCTGTCCC CGGGGCAGAT 
GACTTTGTTC CTGTGTTGGT GTTTGTGTTA ATAAAGGCAA ACCCACCCTG CCTGCTGTCC 
ACTGTGCAGT ACATCAGCAG CTTCTATGCC AGCTGTCTGT CTGGAGAGGA GTCCTACTGG 
TGGATGCAGT TCACAGCAGC CGTGGAGTTT ATTAAAACCA TCGATGACAG AAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAPVD1 ( XM_011239150.3 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_011237452.1 Mus musculus GTPase activating protein and VPS9 domains 1 (Gapvd1), transcript variant X5, mRNA.

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CloneID	OMu69992
Clone ID Related Accession (Same CDS sequence)	XM_011239150.3 , XM_011239150.2
Accession Version	XM_011239150.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	GTPase-activating protein and VPS9 domain-containing protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011239150.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGC TAGATATTCA CACATTGGCC CACCACCTCA AGCAGGAACG CTTATATGTG 
AGCTCTGAGA AACAGCTCAT TCAGAGGCTA AATGCCGATG TACTGAAGAC AGCTGAAAAG 
CTGTATCGAA CAGCGTGGAT TGCAAAGCAA CAGAGGATTA ATTTGGATCG TCTTATTATA 
ACCAGTGCTG AAGCTTCCCC TGCTGAGTGT TGCCAGCATG CCAAGATTTT GGAAGATACA 
CAGTTTGTTG ATGGATATAA GCAACTGGGA TTTCAGGAAA CAGCTTATGG AGAATTCCTG 
AGTCGGTTAA GAGAAAATCC ACGACTTATT GCCTCCTCTT TGGTTGCTGG AGAAAAACTC 
AATCAGGAGA ACACACAAAG TGTTATCTAC ACTGTTTTTA CCTCCTTGTA TGGCAACTGT 
ATTATGCAAG AAGATGAAAG CTACCTCCTT CAGGTTTTAC GATACTTGAT TGAATTTGAA 
CTTAAAGAAA GTGACAACCC CAGGCGCCTT TTGAGGAGAG GAACTTGTGC CTTCAGCATC 
TTATTTAAAC TTTTTTCTGA AGGACTGTTT TCTGCCAAAC TTTTCCTTAC GGCTACTTTG 
CATGAGCCAA TCATGCAGCT CCTCGTAGAG GATGAAGACC ACCTGGAAAC AGATCCAAAC 
AAGCTGATAG AGAGATTCTC TCCAGCTCAG CAAGAAAAAC TCTTTGGAGA GAAAGGCTCA 
GATAGATTTA GGCAAAAGGT TCAAGAAATG GTAGATTCCA ATGAGGCTAA ACTAGTGGCT 
TTGGTGAACA AATTTATTGG TTATCTTAAA CAGAATACAT ACTGCTTTCC ACATAGTTTG 
AGGTGGATTG TGTCACAGAT GTACAAAACT CTTTCTTGTG TAGACAGATT GGAGGTTGGG 
GAAGTCAGAG CAATGTGTAC CGATCTTCTG CTGGCTTGCT TCATTTGTCC TGCAGTTGTC 
AATCCAGAGC AGTATGGGAT AATATCTGAT GCTCCTATTA ATGAGGTGGC AAGATTTAAC 
CTGATGCAGG TTGGCCGCCT TTTGCAGCAG CTAGCAATGA CTGGCACTGA AGAAGGAGAT 
CCCCGGACCA AAAATAGCCT TGGAAAATTT GACAAAGGCT GTGTCGCTGC TTTCCTTGAT 
GTTGTAATTG GGGGCCGTGC AGTGGAGACT CCGCCAATGT CCTCTGTTAA TCTTCTGGAA 
GGCTTGAGCA GAACTGTGGT GTATATAAGT TACAGTCAGC TTATTACTCT GGTAAATTTT 
ATGAAGAGTG TGATGTCTGG AGATCAACTG AAAGAAGATA GAATGGCTCT TGACAATTTG 
TTGGCAAACC TGCCCCAGGC TAAGCCAGGG AAGAGCAGCA GTCTGGACAT GACTCCCTAT 
AGCACTCCTC AGATGTCTCC AGCAACCACT CCAGCCAACA AGAAGAATCG ATTACCTATA 
GCAACTCGGA GCAGAAGCCG AAGTAATATG TTAATGGACC TACATATGGA TCATGAAGGA 
TCATCTCAAG AAACCATCCA GGAGGTACAG CCAGAAGAGG TGCTGGTCAT TTCCTTAGGG 
ACAGGCCCTC AGCTCACTCC AGGGATGATG TCAGAAAATG AGGTTCTAAA CATGCAGCTT 
TCGGATGGAG GACAAGGAGA TGTCCCTGTT GATGAAAACA AACTCCACGG TAAACCTGAT 
AAAACCTTGC GCTTTTCCCT CTGCAGTGAT AATCTGGAAG GCATATCTGA AGGTCCTTCC 
AATCGCTCCA ATTCGGTGTC TTCTCTCGAC CTGGAGGGAG AGTCTGTATC TGAACTTGGG 
GCAGGACCTT CTGGGAGTAA CGGAGTTGAA GCACTGCAGC TGTTAGAGCA CGAGCAAGCT 
ACAACACAAG ATAATCTTGA TGATAAACTG AGGAAGTTTG AGATTCGTGA CATGATGGGA 
CTAACAGATG ACAGGGACAT ATCCGAGACT GTGAGTGAGA CCTGGAGCAC TGATGTCCTG 
GGAAGTGACT TCGACCCGAA CGTTGATGAA GATCGTTTGC AAGAGATTGC AGGTGCAGCA 
GCAGAGAACG TGTTAGGTAG TTTGCTGTGC CTTCCAGGTT CAGGGTCAGT TCTCCTTGAC 
CCTTGCACTG GCTCTACCAT ATCAGAGACC ACAAGTGAAG CTTGGAGTGT AGAGGTATTG 
CCAAGTGACT CAGAGGCTCC AGACTTAAAG CAGGAAGAGC GCCTCCAAGA GCTGGAGAGC 
TGTTCTGGAC TGGGCAGCAC ATCTGATGAT ACGGATGTCA GGGAGGTCAG TTCCCGCCCC 
AGCACACCAG GCCTCAGTGT TGTGTCCGGT ATAAGTGCTA CATCGGAGGA TATTCCCAAT 
AAGATCGAAG ACCTGAGATC TGAGTGCAGC TCTGATTTTG GGGGCAAAGA TTCTGTAACT 
AGTCCAGATA TGGATGACAT AGCCCATGGC GCTCATCAGC TGACCTCTCC TCCCTCTCAG 
TCAGAGTCTC TGCTTGCCAT GTTTGATCCA CTGTCCTCAC ATGAAGGGGC CTCTGCTGTG 
GTAAGGCCAA AGGTTCACTA TGCCCGACCC TCGCATCCAC CTCCGGATCC TCCAATCCTG 
GAAGGTGCAG TGGGAGGAAA TGAGGCCAGG TTGCCAAACT TTGGCTCTCA CGTTTTAACT 
GCAGCTGAAA TGGAGGCGTT CAAGCAGAGA CACTCATACC CTGAGAGATT GGTTCGCAGC 
AGAAGCTCTG ATATAGTGTC TTCTGTGCGG CGGCCCATGA GTGACCCCAG CTGGAACCGA 
CGTCCAGGGA ATGAAGAACT TCCTCCAGCT GCAGCCACTG GTGCTACTTC TTTGGTGGCT 
GCACCTCACT CGTCATCTTC GTCCCCGAGT AAGGACTCTT CTAGAGGAGA GACTGAGGAA 
CGCAAGGACA GTGACGATGA GAGGTCAGAC AGGAGCAGGC CTTGGTGGAG GAAGCGCTTT 
GTTTCAGCTA TGCCTAAAGA TGAGCCCAGC CCTAGGCTCA GTGCTCAAGC TCAGGTTGCT 
GAAGATATTC TGGACAAGTA CCGGAATGCC ATTAAACGGA CCAGCCCCAG TGAAGGAGCA 
ATGGCAAATG ATGAGAGTGC AGAGGTCATG GGTGATGGTG AAAGTGCCCA CGACTCTCCT 
CGTGAGGAAG CACTGCAGAA CATCTCAGCC GACGACCTCC CGGACTCCGC CAGCCAGGCA 
GCCCACCCTC AGGACTCTGC TTTCTCTTAC AGAGATGTAA AAAAGAAGCT GAGGCTCGCA 
CTTTGCTCTG CAGACTCTGT GGCCTTCCCA GTGCTAACTC ATTCAACAAG GAATGGATTG 
CCAGACCACA CAGACCCAGA AGACAATGAA ATTGTATGCT TCTTAAAAGT TCAAATAGCT 
GAAGCAATTA ATTTACAAGA TAAGAGCTTA ATGGCTCAAC TTCAAGAAAC AATGCGTTGT 
GTGTGCCGCT TTGACAATAG GACTTGTAGG AAGTTGCTGG CATCCATTGC TGAAGACTAC 
AGAAAAAGAG CTCCGTATAT TGCTTATCTT ACCCGCTGCC GACAAGGACT GCAGACCACA 
CAGGCTCACT TGGAAAGGCT CTTGCAAAGA GTTTTGCGGG ACAAAGAGGT GGCCAATCGG 
TACTTCACCA CTGTCTGTGT GCGGTTACTG CTTGAGAGCA AAGAAAAGAA AATCAGGGAG 
TTCATTCAAG ACTTTCAGAA ACTTACTGCT GCTGATGATA AAACGGCTCA GGTGGAGGAT 
TTTCTACAGT TCCTGTATGG TGTGATGGCT CAGGATGTCA TATGGCAGAA TGCCAGTGAG 
GAGCAGCTGC AGGATGCCCA GCTGGCCATC GAGCGGAGTG TGATGAATCG GATCTTCAAG 
CTTGCTTTCT ACCCTAACCA GGATGGGGAC ATACTTCGTG ACCAGGTTCT GCATGAACAT 
ATCCAGAGAT TATCTAAAGT AGTGACTGCA AACCACAGAG CTCTGCAGAT CCCTGAGGTT 
TATCTCCGTG AGGCACCATG GCCATCTGCA CAGTCAGAAA TCAGGACAAT AAGTGCTTAC 
AAAACGCCCC GCGACAAAGT GCAGTGCATC CTGAGGATGT GCTCCACCAT CATGAACCTC 
TTGAGCCTGG CCAACGAGGA CTCTGTCCCC GGGGCAGATG ACTTTGTTCC TGTGTTGGTG 
TTTGTGTTAA TAAAGGCAAA CCCACCCTGC CTGCTGTCCA CTGTGCAGTA CATCAGCAGC 
TTCTATGCCA GCTGTCTGTC TGGAGAGGAG TCCTACTGGT GGATGCAGTT CACAGCAGCC 
GTGGAGTTTA TTAAAACCAT CGATGACAGA AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011237452.1
CDS	636..4931
Translation	MVKLDIHTLAHHLKQERLYVSSEKQLIQRLNADVLKTAEKLYRTAWIAKQQRINLDRLIITSAEASPAECCQHAKILEDTQFVDGYKQLGFQETAYGEFLSRLRENPRLIASSLVAGEKLNQENTQSVIYTVFTSLYGNCIMQEDESYLLQVLRYLIEFELKESDNPRRLLRRGTCAFSILFKLFSEGLFSAKLFLTATLHEPIMQLLVEDEDHLETDPNKLIERFSPAQQEKLFGEKGSDRFRQKVQEMVDSNEAKLVALVNKFIGYLKQNTYCFPHSLRWIVSQMYKTLSCVDRLEVGEVRAMCTDLLLACFICPAVVNPEQYGIISDAPINEVARFNLMQVGRLLQQLAMTGTEEGDPRTKNSLGKFDKGCVAAFLDVVIGGRAVETPPMSSVNLLEGLSRTVVYISYSQLITLVNFMKSVMSGDQLKEDRMALDNLLANLPQAKPGKSSSLDMTPYSTPQMSPATTPANKKNRLPIATRSRSRSNMLMDLHMDHEGSSQETIQEVQPEEVLVISLGTGPQLTPGMMSENEVLNMQLSDGGQGDVPVDENKLHGKPDKTLRFSLCSDNLEGISEGPSNRSNSVSSLDLEGESVSELGAGPSGSNGVEALQLLEHEQATTQDNLDDKLRKFEIRDMMGLTDDRDISETVSETWSTDVLGSDFDPNVDEDRLQEIAGAAAENVLGSLLCLPGSGSVLLDPCTGSTISETTSEAWSVEVLPSDSEAPDLKQEERLQELESCSGLGSTSDDTDVREVSSRPSTPGLSVVSGISATSEDIPNKIEDLRSECSSDFGGKDSVTSPDMDDIAHGAHQLTSPPSQSESLLAMFDPLSSHEGASAVVRPKVHYARPSHPPPDPPILEGAVGGNEARLPNFGSHVLTAAEMEAFKQRHSYPERLVRSRSSDIVSSVRRPMSDPSWNRRPGNEELPPAAATGATSLVAAPHSSSSSPSKDSSRGETEERKDSDDERSDRSRPWWRKRFVSAMPKDEPSPRLSAQAQVAEDILDKYRNAIKRTSPSEGAMANDESAEVMGDGESAHDSPREEALQNISADDLPDSASQAAHPQDSAFSYRDVKKKLRLALCSADSVAFPVLTHSTRNGLPDHTDPEDNEIVCFLKVQIAEAINLQDKSLMAQLQETMRCVCRFDNRTCRKLLASIAEDYRKRAPYIAYLTRCRQGLQTTQAHLERLLQRVLRDKEVANRYFTTVCVRLLLESKEKKIREFIQDFQKLTAADDKTAQVEDFLQFLYGVMAQDVIWQNASEEQLQDAQLAIERSVMNRIFKLAFYPNQDGDILRDQVLHEHIQRLSKVVTANHRALQIPEVYLREAPWPSAQSEIRTISAYKTPRDKVQCILRMCSTIMNLLSLANEDSVPGADDFVPVLVFVLIKANPPCLLSTVQYISSFYASCLSGEESYWWMQFTAAVEFIKTIDDRK

Target ORF information:

RefSeq Version	XM_011239150.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus GTPase activating protein and VPS9 domains 1 (Gapvd1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011239150.3

ORF Insert Sequence:

ATGGTGAAGC TAGATATTCA CACATTGGCC CACCACCTCA AGCAGGAACG CTTATATGTG 
AGCTCTGAGA AACAGCTCAT TCAGAGGCTA AATGCCGATG TACTGAAGAC AGCTGAAAAG 
CTGTATCGAA CAGCGTGGAT TGCAAAGCAA CAGAGGATTA ATTTGGATCG TCTTATTATA 
ACCAGTGCTG AAGCTTCCCC TGCTGAGTGT TGCCAGCATG CCAAGATTTT GGAAGATACA 
CAGTTTGTTG ATGGATATAA GCAACTGGGA TTTCAGGAAA CAGCTTATGG AGAATTCCTG 
AGTCGGTTAA GAGAAAATCC ACGACTTATT GCCTCCTCTT TGGTTGCTGG AGAAAAACTC 
AATCAGGAGA ACACACAAAG TGTTATCTAC ACTGTTTTTA CCTCCTTGTA TGGCAACTGT 
ATTATGCAAG AAGATGAAAG CTACCTCCTT CAGGTTTTAC GATACTTGAT TGAATTTGAA 
CTTAAAGAAA GTGACAACCC CAGGCGCCTT TTGAGGAGAG GAACTTGTGC CTTCAGCATC 
TTATTTAAAC TTTTTTCTGA AGGACTGTTT TCTGCCAAAC TTTTCCTTAC GGCTACTTTG 
CATGAGCCAA TCATGCAGCT CCTCGTAGAG GATGAAGACC ACCTGGAAAC AGATCCAAAC 
AAGCTGATAG AGAGATTCTC TCCAGCTCAG CAAGAAAAAC TCTTTGGAGA GAAAGGCTCA 
GATAGATTTA GGCAAAAGGT TCAAGAAATG GTAGATTCCA ATGAGGCTAA ACTAGTGGCT 
TTGGTGAACA AATTTATTGG TTATCTTAAA CAGAATACAT ACTGCTTTCC ACATAGTTTG 
AGGTGGATTG TGTCACAGAT GTACAAAACT CTTTCTTGTG TAGACAGATT GGAGGTTGGG 
GAAGTCAGAG CAATGTGTAC CGATCTTCTG CTGGCTTGCT TCATTTGTCC TGCAGTTGTC 
AATCCAGAGC AGTATGGGAT AATATCTGAT GCTCCTATTA ATGAGGTGGC AAGATTTAAC 
CTGATGCAGG TTGGCCGCCT TTTGCAGCAG CTAGCAATGA CTGGCACTGA AGAAGGAGAT 
CCCCGGACCA AAAATAGCCT TGGAAAATTT GACAAAGGCT GTGTCGCTGC TTTCCTTGAT 
GTTGTAATTG GGGGCCGTGC AGTGGAGACT CCGCCAATGT CCTCTGTTAA TCTTCTGGAA 
GGCTTGAGCA GAACTGTGGT GTATATAAGT TACAGTCAGC TTATTACTCT GGTAAATTTT 
ATGAAGAGTG TGATGTCTGG AGATCAACTG AAAGAAGATA GAATGGCTCT TGACAATTTG 
TTGGCAAACC TGCCCCAGGC TAAGCCAGGG AAGAGCAGCA GTCTGGACAT GACTCCCTAT 
AGCACTCCTC AGATGTCTCC AGCAACCACT CCAGCCAACA AGAAGAATCG ATTACCTATA 
GCAACTCGGA GCAGAAGCCG AAGTAATATG TTAATGGACC TACATATGGA TCATGAAGGA 
TCATCTCAAG AAACCATCCA GGAGGTACAG CCAGAAGAGG TGCTGGTCAT TTCCTTAGGG 
ACAGGCCCTC AGCTCACTCC AGGGATGATG TCAGAAAATG AGGTTCTAAA CATGCAGCTT 
TCGGATGGAG GACAAGGAGA TGTCCCTGTT GATGAAAACA AACTCCACGG TAAACCTGAT 
AAAACCTTGC GCTTTTCCCT CTGCAGTGAT AATCTGGAAG GCATATCTGA AGGTCCTTCC 
AATCGCTCCA ATTCGGTGTC TTCTCTCGAC CTGGAGGGAG AGTCTGTATC TGAACTTGGG 
GCAGGACCTT CTGGGAGTAA CGGAGTTGAA GCACTGCAGC TGTTAGAGCA CGAGCAAGCT 
ACAACACAAG ATAATCTTGA TGATAAACTG AGGAAGTTTG AGATTCGTGA CATGATGGGA 
CTAACAGATG ACAGGGACAT ATCCGAGACT GTGAGTGAGA CCTGGAGCAC TGATGTCCTG 
GGAAGTGACT TCGACCCGAA CGTTGATGAA GATCGTTTGC AAGAGATTGC AGGTGCAGCA 
GCAGAGAACG TGTTAGGTAG TTTGCTGTGC CTTCCAGGTT CAGGGTCAGT TCTCCTTGAC 
CCTTGCACTG GCTCTACCAT ATCAGAGACC ACAAGTGAAG CTTGGAGTGT AGAGGTATTG 
CCAAGTGACT CAGAGGCTCC AGACTTAAAG CAGGAAGAGC GCCTCCAAGA GCTGGAGAGC 
TGTTCTGGAC TGGGCAGCAC ATCTGATGAT ACGGATGTCA GGGAGGTCAG TTCCCGCCCC 
AGCACACCAG GCCTCAGTGT TGTGTCCGGT ATAAGTGCTA CATCGGAGGA TATTCCCAAT 
AAGATCGAAG ACCTGAGATC TGAGTGCAGC TCTGATTTTG GGGGCAAAGA TTCTGTAACT 
AGTCCAGATA TGGATGACAT AGCCCATGGC GCTCATCAGC TGACCTCTCC TCCCTCTCAG 
TCAGAGTCTC TGCTTGCCAT GTTTGATCCA CTGTCCTCAC ATGAAGGGGC CTCTGCTGTG 
GTAAGGCCAA AGGTTCACTA TGCCCGACCC TCGCATCCAC CTCCGGATCC TCCAATCCTG 
GAAGGTGCAG TGGGAGGAAA TGAGGCCAGG TTGCCAAACT TTGGCTCTCA CGTTTTAACT 
GCAGCTGAAA TGGAGGCGTT CAAGCAGAGA CACTCATACC CTGAGAGATT GGTTCGCAGC 
AGAAGCTCTG ATATAGTGTC TTCTGTGCGG CGGCCCATGA GTGACCCCAG CTGGAACCGA 
CGTCCAGGGA ATGAAGAACT TCCTCCAGCT GCAGCCACTG GTGCTACTTC TTTGGTGGCT 
GCACCTCACT CGTCATCTTC GTCCCCGAGT AAGGACTCTT CTAGAGGAGA GACTGAGGAA 
CGCAAGGACA GTGACGATGA GAGGTCAGAC AGGAGCAGGC CTTGGTGGAG GAAGCGCTTT 
GTTTCAGCTA TGCCTAAAGA TGAGCCCAGC CCTAGGCTCA GTGCTCAAGC TCAGGTTGCT 
GAAGATATTC TGGACAAGTA CCGGAATGCC ATTAAACGGA CCAGCCCCAG TGAAGGAGCA 
ATGGCAAATG ATGAGAGTGC AGAGGTCATG GGTGATGGTG AAAGTGCCCA CGACTCTCCT 
CGTGAGGAAG CACTGCAGAA CATCTCAGCC GACGACCTCC CGGACTCCGC CAGCCAGGCA 
GCCCACCCTC AGGACTCTGC TTTCTCTTAC AGAGATGTAA AAAAGAAGCT GAGGCTCGCA 
CTTTGCTCTG CAGACTCTGT GGCCTTCCCA GTGCTAACTC ATTCAACAAG GAATGGATTG 
CCAGACCACA CAGACCCAGA AGACAATGAA ATTGTATGCT TCTTAAAAGT TCAAATAGCT 
GAAGCAATTA ATTTACAAGA TAAGAGCTTA ATGGCTCAAC TTCAAGAAAC AATGCGTTGT 
GTGTGCCGCT TTGACAATAG GACTTGTAGG AAGTTGCTGG CATCCATTGC TGAAGACTAC 
AGAAAAAGAG CTCCGTATAT TGCTTATCTT ACCCGCTGCC GACAAGGACT GCAGACCACA 
CAGGCTCACT TGGAAAGGCT CTTGCAAAGA GTTTTGCGGG ACAAAGAGGT GGCCAATCGG 
TACTTCACCA CTGTCTGTGT GCGGTTACTG CTTGAGAGCA AAGAAAAGAA AATCAGGGAG 
TTCATTCAAG ACTTTCAGAA ACTTACTGCT GCTGATGATA AAACGGCTCA GGTGGAGGAT 
TTTCTACAGT TCCTGTATGG TGTGATGGCT CAGGATGTCA TATGGCAGAA TGCCAGTGAG 
GAGCAGCTGC AGGATGCCCA GCTGGCCATC GAGCGGAGTG TGATGAATCG GATCTTCAAG 
CTTGCTTTCT ACCCTAACCA GGATGGGGAC ATACTTCGTG ACCAGGTTCT GCATGAACAT 
ATCCAGAGAT TATCTAAAGT AGTGACTGCA AACCACAGAG CTCTGCAGAT CCCTGAGGTT 
TATCTCCGTG AGGCACCATG GCCATCTGCA CAGTCAGAAA TCAGGACAAT AAGTGCTTAC 
AAAACGCCCC GCGACAAAGT GCAGTGCATC CTGAGGATGT GCTCCACCAT CATGAACCTC 
TTGAGCCTGG CCAACGAGGA CTCTGTCCCC GGGGCAGATG ACTTTGTTCC TGTGTTGGTG 
TTTGTGTTAA TAAAGGCAAA CCCACCCTGC CTGCTGTCCA CTGTGCAGTA CATCAGCAGC 
TTCTATGCCA GCTGTCTGTC TGGAGAGGAG TCCTACTGGT GGATGCAGTT CACAGCAGCC 
GTGGAGTTTA TTAAAACCAT CGATGACAGA AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAPVD1 ( XM_030251956.1 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_030107816.1 Mus musculus GTPase activating protein and VPS9 domains 1 (Gapvd1), transcript variant X6, mRNA.

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CloneID	OMu87968
Clone ID Related Accession (Same CDS sequence)	XM_030251956.1
Accession Version	XM_030251956.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	GTPase-activating protein and VPS9 domain-containing protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGC TAGATATTCA CACATTGGCC CACCACCTCA AGCAGGAACG CTTATATGTG 
AGCTCTGAGA AACAGCTCAT TCAGAGGCTA AATGCCGATG TACTGAAGAC AGCTGAAAAG 
CTGTATCGAA CAGCGTGGAT TGCAAAGCAA CAGAGGATTA ATTTGGATCG TCTTATTATA 
ACCAGTGCTG AAGCTTCCCC TGCTGAGTGT TGCCAGCATG CCAAGATTTT GGAAGATACA 
CAGTTTGTTG ATGGATATAA GCAACTGGGA TTTCAGGAAA CAGCTTATGG AGAATTCCTG 
AGTCGGTTAA GAGAAAATCC ACGACTTATT GCCTCCTCTT TGGTTGCTGG AGAAAAACTC 
AATCAGGAGA ACACACAAAG TGTTATCTAC ACTGTTTTTA CCTCCTTGTA TGGCAACTGT 
ATTATGCAAG AAGATGAAAG CTACCTCCTT CAGGTTTTAC GATACTTGAT TGAATTTGAA 
CTTAAAGAAA GTGACAACCC CAGGCGCCTT TTGAGGAGAG GAACTTGTGC CTTCAGCATC 
TTATTTAAAC TTTTTTCTGA AGGACTGTTT TCTGCCAAAC TTTTCCTTAC GGCTACTTTG 
CATGAGCCAA TCATGCAGCT CCTCGTAGAG GATGAAGACC ACCTGGAAAC AGATCCAAAC 
AAGCTGATAG AGAGATTCTC TCCAGCTCAG CAAGAAAAAC TCTTTGGAGA GAAAGGCTCA 
GATAGATTTA GGCAAAAGGT TCAAGAAATG GTAGATTCCA ATGAGGCTAA ACTAGTGGCT 
TTGGTGAACA AATTTATTGG TTATCTTAAA CAGAATACAT ACTGCTTTCC ACATAGTTTG 
AGGTGGATTG TGTCACAGAT GTACAAAACT CTTTCTTGTG TAGACAGATT GGAGGTTGGG 
GAAGTCAGAG CAATGTGTAC CGATCTTCTG CTGGCTTGCT TCATTTGTCC TGCAGTTGTC 
AATCCAGAGC AGTATGGGAT AATATCTGAT GCTCCTATTA ATGAGGTGGC AAGATTTAAC 
CTGATGCAGG TTGGCCGCCT TTTGCAGCAG CTAGCAATGA CTGGCACTGA AGAAGGAGAT 
CCCCGGACCA AAAATAGCCT TGGAAAATTT GACAAAGGCT GTGTCGCTGC TTTCCTTGAT 
GTTGTAATTG GGGGCCGTGC AGTGGAGACT CCGCCAATGT CCTCTGTTAA TCTTCTGGAA 
GGCTTGAGCA GAACTGTGGT GTATATAAGT TACAGTCAGC TTATTACTCT GGTAAATTTT 
ATGAAGAGTG TGATGTCTGG AGATCAACTG AAAGAAGATA GAATGGCTCT TGACAATTTG 
TTGGCAAACC TGCCCCAGGC TAAGCCAGGG AAGAGCAGCA GTCTGGACAT GACTCCCTAT 
AGCACTCCTC AGATGTCTCC AGCAACCACT CCAGCCAACA AGAAGAATCG ATTACCTATA 
GCAACTCGGA GCAGAAGCCG AAGTAATATG TTAATGGACC TACATATGGA TCATGAAGGA 
TCATCTCAAG AAACCATCCA GGAGGTACAG CCAGAAGAGG TGCTGGTCAT TTCCTTAGGG 
ACAGGCCCTC AGCTCACTCC AGGGATGATG TCAGAAAATG AGGTTCTAAA CATGCAGCTT 
TCGGATGGAG GACAAGGAGA TGTCCCTGTT GATGAAAACA AACTCCACGG TCCTTCCAAT 
CGCTCCAATT CGGTGTCTTC TCTCGACCTG GAGGGAGAGT CTGTATCTGA ACTTGGGGCA 
GGACCTTCTG GGAGTAACGG AGTTGAAGCA CTGCAGCTGT TAGAGCACGA GCAAGCTACA 
ACACAAGATA ATCTTGATGA TAAACTGAGG AAGTTTGAGA TTCGTGACAT GATGGGACTA 
ACAGATGACA GGGACATATC CGAGACTGTG AGTGAGACCT GGAGCACTGA TGTCCTGGGA 
AGTGACTTCG ACCCGAACGT TGATGAAGAT CGTTTGCAAG AGATTGCAGG TGCAGCAGCA 
GAGAACGTGT TAGGTAGTTT GCTGTGCCTT CCAGGTTCAG GGTCAGTTCT CCTTGACCCT 
TGCACTGGCT CTACCATATC AGAGACCACA AGTGAAGCTT GGAGTGTAGA GGTATTGCCA 
AGTGACTCAG AGGCTCCAGA CTTAAAGCAG GAAGAGCGCC TCCAAGAGCT GGAGAGCTGT 
TCTGGACTGG GCAGCACATC TGATGATACG GATGTCAGGG AGGTCAGTTC CCGCCCCAGC 
ACACCAGGCC TCAGTGTTGT GTCCGGTATA AGTGCTACAT CGGAGGATAT TCCCAATAAG 
ATCGAAGACC TGAGATCTGA GTGCAGCTCT GATTTTGGGG GCAAAGATTC TGTAACTAGT 
CCAGATATGG ATGACATAGC CCATGGCGCT CATCAGCTGA CCTCTCCTCC CTCTCAGTCA 
GAGTCTCTGC TTGCCATGTT TGATCCACTG TCCTCACATG AAGGGGCCTC TGCTGTGGTA 
AGGCCAAAGG TTCACTATGC CCGACCCTCG CATCCACCTC CGGATCCTCC AATCCTGGAA 
GGTGCAGTGG GAGGAAATGA GGCCAGGTTG CCAAACTTTG GCTCTCACGT TTTAACTGCA 
GCTGAAATGG AGGCGTTCAA GCAGAGACAC TCATACCCTG AGAGATTGGT TCGCAGCAGA 
AGCTCTGATA TAGTGTCTTC TGTGCGGCGG CCCATGAGTG ACCCCAGCTG GAACCGACGT 
CCAGGGAATG AAGAACTTCC TCCAGCTGCA GCCACTGGTG CTACTTCTTT GGTGGCTGCA 
CCTCACTCGT CATCTTCGTC CCCGAGTAAG GACTCTTCTA GAGGAGAGAC TGAGGAACGC 
AAGGACAGTG ACGATGAGAG GTCAGACAGG AGCAGGCCTT GGTGGAGGAA GCGCTTTGTT 
TCAGCTATGC CTAAAGATGA GCCCAGCCCT AGGCTCAGTG CTCAAGCTCA GGTTGCTGAA 
GATATTCTGG ACAAGTACCG GAATGCCATT AAACGGACCA GCCCCAGTGA AGGAGCAATG 
GCAAATGATG AGAGTGCAGA GGTCATGGGT GATGGTGAAA GTGCCCACGA CTCTCCTCGT 
GAGGAAGCAC TGCAGAACAT CTCAGCCGAC GACCTCCCGG ACTCCGCCAG CCAGGCAGCC 
CACCCTCAGG ACTCTGCTTT CTCTTACAGA GATGTAAAAA AGAAGCTGAG GCTCGCACTT 
TGCTCTGCAG ACTCTGTGGC CTTCCCAGTG CTAACTCATT CAACAAGGAA TGGATTGCCA 
GACCACACAG ACCCAGAAGA CAATGAAATT GTATGCTTCT TAAAAGTTCA AATAGCTGAA 
GCAATTAATT TACAAGATAA GAGCTTAATG GCTCAACTTC AAGAAACAAT GCGTTGTGTG 
TGCCGCTTTG ACAATAGGAC TTGTAGGAAG TTGCTGGCAT CCATTGCTGA AGACTACAGA 
AAAAGAGCTC CGTATATTGC TTATCTTACC CGCTGCCGAC AAGGACTGCA GACCACACAG 
GCTCACTTGG AAAGGCTCTT GCAAAGAGTT TTGCGGGACA AAGAGGTGGC CAATCGGTAC 
TTCACCACTG TCTGTGTGCG GTTACTGCTT GAGAGCAAAG AAAAGAAAAT CAGGGAGTTC 
ATTCAAGACT TTCAGAAACT TACTGCTGCT GATGATAAAA CGGCTCAGGT GGAGGATTTT 
CTACAGTTCC TGTATGGTGT GATGGCTCAG GATGTCATAT GGCAGAATGC CAGTGAGGAG 
CAGCTGCAGG ATGCCCAGCT GGCCATCGAG CGGAGTGTGA TGAATCGGAT CTTCAAGCTT 
GCTTTCTACC CTAACCAGGA TGGGGACATA CTTCGTGACC AGGTTCTGCA TGAACATATC 
CAGAGATTAT CTAAAGTAGT GACTGCAAAC CACAGAGCTC TGCAGATCCC TGAGGTTTAT 
CTCCGTGAGG CACCATGGCC ATCTGCACAG TCAGAAATCA GGACAATAAG TGCTTACAAA 
ACGCCCCGCG ACAAAGTGCA GTGCATCCTG AGGATGTGCT CCACCATCAT GAACCTCTTG 
AGCCTGGCCA ACGAGGACTC TGTCCCCGGG GCAGATGACT TTGTTCCTGT GTTGGTGTTT 
GTGTTAATAA AGGCAAACCC ACCCTGCCTG CTGTCCACTG TGCAGTACAT CAGCAGCTTC 
TATGCCAGCT GTCTGTCTGG AGAGGAGTCC TACTGGTGGA TGCAGTTCAC AGCAGCCGTG 
GAGTTTATTA AAACCATCGA TGACAGAAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030107816.1
CDS	592..4824
Translation	MVKLDIHTLAHHLKQERLYVSSEKQLIQRLNADVLKTAEKLYRTAWIAKQQRINLDRLIITSAEASPAECCQHAKILEDTQFVDGYKQLGFQETAYGEFLSRLRENPRLIASSLVAGEKLNQENTQSVIYTVFTSLYGNCIMQEDESYLLQVLRYLIEFELKESDNPRRLLRRGTCAFSILFKLFSEGLFSAKLFLTATLHEPIMQLLVEDEDHLETDPNKLIERFSPAQQEKLFGEKGSDRFRQKVQEMVDSNEAKLVALVNKFIGYLKQNTYCFPHSLRWIVSQMYKTLSCVDRLEVGEVRAMCTDLLLACFICPAVVNPEQYGIISDAPINEVARFNLMQVGRLLQQLAMTGTEEGDPRTKNSLGKFDKGCVAAFLDVVIGGRAVETPPMSSVNLLEGLSRTVVYISYSQLITLVNFMKSVMSGDQLKEDRMALDNLLANLPQAKPGKSSSLDMTPYSTPQMSPATTPANKKNRLPIATRSRSRSNMLMDLHMDHEGSSQETIQEVQPEEVLVISLGTGPQLTPGMMSENEVLNMQLSDGGQGDVPVDENKLHGPSNRSNSVSSLDLEGESVSELGAGPSGSNGVEALQLLEHEQATTQDNLDDKLRKFEIRDMMGLTDDRDISETVSETWSTDVLGSDFDPNVDEDRLQEIAGAAAENVLGSLLCLPGSGSVLLDPCTGSTISETTSEAWSVEVLPSDSEAPDLKQEERLQELESCSGLGSTSDDTDVREVSSRPSTPGLSVVSGISATSEDIPNKIEDLRSECSSDFGGKDSVTSPDMDDIAHGAHQLTSPPSQSESLLAMFDPLSSHEGASAVVRPKVHYARPSHPPPDPPILEGAVGGNEARLPNFGSHVLTAAEMEAFKQRHSYPERLVRSRSSDIVSSVRRPMSDPSWNRRPGNEELPPAAATGATSLVAAPHSSSSSPSKDSSRGETEERKDSDDERSDRSRPWWRKRFVSAMPKDEPSPRLSAQAQVAEDILDKYRNAIKRTSPSEGAMANDESAEVMGDGESAHDSPREEALQNISADDLPDSASQAAHPQDSAFSYRDVKKKLRLALCSADSVAFPVLTHSTRNGLPDHTDPEDNEIVCFLKVQIAEAINLQDKSLMAQLQETMRCVCRFDNRTCRKLLASIAEDYRKRAPYIAYLTRCRQGLQTTQAHLERLLQRVLRDKEVANRYFTTVCVRLLLESKEKKIREFIQDFQKLTAADDKTAQVEDFLQFLYGVMAQDVIWQNASEEQLQDAQLAIERSVMNRIFKLAFYPNQDGDILRDQVLHEHIQRLSKVVTANHRALQIPEVYLREAPWPSAQSEIRTISAYKTPRDKVQCILRMCSTIMNLLSLANEDSVPGADDFVPVLVFVLIKANPPCLLSTVQYISSFYASCLSGEESYWWMQFTAAVEFIKTIDDRK

Target ORF information:

RefSeq Version	XM_030251956.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus GTPase activating protein and VPS9 domains 1 (Gapvd1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030251956.1

ORF Insert Sequence:

ATGGTGAAGC TAGATATTCA CACATTGGCC CACCACCTCA AGCAGGAACG CTTATATGTG 
AGCTCTGAGA AACAGCTCAT TCAGAGGCTA AATGCCGATG TACTGAAGAC AGCTGAAAAG 
CTGTATCGAA CAGCGTGGAT TGCAAAGCAA CAGAGGATTA ATTTGGATCG TCTTATTATA 
ACCAGTGCTG AAGCTTCCCC TGCTGAGTGT TGCCAGCATG CCAAGATTTT GGAAGATACA 
CAGTTTGTTG ATGGATATAA GCAACTGGGA TTTCAGGAAA CAGCTTATGG AGAATTCCTG 
AGTCGGTTAA GAGAAAATCC ACGACTTATT GCCTCCTCTT TGGTTGCTGG AGAAAAACTC 
AATCAGGAGA ACACACAAAG TGTTATCTAC ACTGTTTTTA CCTCCTTGTA TGGCAACTGT 
ATTATGCAAG AAGATGAAAG CTACCTCCTT CAGGTTTTAC GATACTTGAT TGAATTTGAA 
CTTAAAGAAA GTGACAACCC CAGGCGCCTT TTGAGGAGAG GAACTTGTGC CTTCAGCATC 
TTATTTAAAC TTTTTTCTGA AGGACTGTTT TCTGCCAAAC TTTTCCTTAC GGCTACTTTG 
CATGAGCCAA TCATGCAGCT CCTCGTAGAG GATGAAGACC ACCTGGAAAC AGATCCAAAC 
AAGCTGATAG AGAGATTCTC TCCAGCTCAG CAAGAAAAAC TCTTTGGAGA GAAAGGCTCA 
GATAGATTTA GGCAAAAGGT TCAAGAAATG GTAGATTCCA ATGAGGCTAA ACTAGTGGCT 
TTGGTGAACA AATTTATTGG TTATCTTAAA CAGAATACAT ACTGCTTTCC ACATAGTTTG 
AGGTGGATTG TGTCACAGAT GTACAAAACT CTTTCTTGTG TAGACAGATT GGAGGTTGGG 
GAAGTCAGAG CAATGTGTAC CGATCTTCTG CTGGCTTGCT TCATTTGTCC TGCAGTTGTC 
AATCCAGAGC AGTATGGGAT AATATCTGAT GCTCCTATTA ATGAGGTGGC AAGATTTAAC 
CTGATGCAGG TTGGCCGCCT TTTGCAGCAG CTAGCAATGA CTGGCACTGA AGAAGGAGAT 
CCCCGGACCA AAAATAGCCT TGGAAAATTT GACAAAGGCT GTGTCGCTGC TTTCCTTGAT 
GTTGTAATTG GGGGCCGTGC AGTGGAGACT CCGCCAATGT CCTCTGTTAA TCTTCTGGAA 
GGCTTGAGCA GAACTGTGGT GTATATAAGT TACAGTCAGC TTATTACTCT GGTAAATTTT 
ATGAAGAGTG TGATGTCTGG AGATCAACTG AAAGAAGATA GAATGGCTCT TGACAATTTG 
TTGGCAAACC TGCCCCAGGC TAAGCCAGGG AAGAGCAGCA GTCTGGACAT GACTCCCTAT 
AGCACTCCTC AGATGTCTCC AGCAACCACT CCAGCCAACA AGAAGAATCG ATTACCTATA 
GCAACTCGGA GCAGAAGCCG AAGTAATATG TTAATGGACC TACATATGGA TCATGAAGGA 
TCATCTCAAG AAACCATCCA GGAGGTACAG CCAGAAGAGG TGCTGGTCAT TTCCTTAGGG 
ACAGGCCCTC AGCTCACTCC AGGGATGATG TCAGAAAATG AGGTTCTAAA CATGCAGCTT 
TCGGATGGAG GACAAGGAGA TGTCCCTGTT GATGAAAACA AACTCCACGG TCCTTCCAAT 
CGCTCCAATT CGGTGTCTTC TCTCGACCTG GAGGGAGAGT CTGTATCTGA ACTTGGGGCA 
GGACCTTCTG GGAGTAACGG AGTTGAAGCA CTGCAGCTGT TAGAGCACGA GCAAGCTACA 
ACACAAGATA ATCTTGATGA TAAACTGAGG AAGTTTGAGA TTCGTGACAT GATGGGACTA 
ACAGATGACA GGGACATATC CGAGACTGTG AGTGAGACCT GGAGCACTGA TGTCCTGGGA 
AGTGACTTCG ACCCGAACGT TGATGAAGAT CGTTTGCAAG AGATTGCAGG TGCAGCAGCA 
GAGAACGTGT TAGGTAGTTT GCTGTGCCTT CCAGGTTCAG GGTCAGTTCT CCTTGACCCT 
TGCACTGGCT CTACCATATC AGAGACCACA AGTGAAGCTT GGAGTGTAGA GGTATTGCCA 
AGTGACTCAG AGGCTCCAGA CTTAAAGCAG GAAGAGCGCC TCCAAGAGCT GGAGAGCTGT 
TCTGGACTGG GCAGCACATC TGATGATACG GATGTCAGGG AGGTCAGTTC CCGCCCCAGC 
ACACCAGGCC TCAGTGTTGT GTCCGGTATA AGTGCTACAT CGGAGGATAT TCCCAATAAG 
ATCGAAGACC TGAGATCTGA GTGCAGCTCT GATTTTGGGG GCAAAGATTC TGTAACTAGT 
CCAGATATGG ATGACATAGC CCATGGCGCT CATCAGCTGA CCTCTCCTCC CTCTCAGTCA 
GAGTCTCTGC TTGCCATGTT TGATCCACTG TCCTCACATG AAGGGGCCTC TGCTGTGGTA 
AGGCCAAAGG TTCACTATGC CCGACCCTCG CATCCACCTC CGGATCCTCC AATCCTGGAA 
GGTGCAGTGG GAGGAAATGA GGCCAGGTTG CCAAACTTTG GCTCTCACGT TTTAACTGCA 
GCTGAAATGG AGGCGTTCAA GCAGAGACAC TCATACCCTG AGAGATTGGT TCGCAGCAGA 
AGCTCTGATA TAGTGTCTTC TGTGCGGCGG CCCATGAGTG ACCCCAGCTG GAACCGACGT 
CCAGGGAATG AAGAACTTCC TCCAGCTGCA GCCACTGGTG CTACTTCTTT GGTGGCTGCA 
CCTCACTCGT CATCTTCGTC CCCGAGTAAG GACTCTTCTA GAGGAGAGAC TGAGGAACGC 
AAGGACAGTG ACGATGAGAG GTCAGACAGG AGCAGGCCTT GGTGGAGGAA GCGCTTTGTT 
TCAGCTATGC CTAAAGATGA GCCCAGCCCT AGGCTCAGTG CTCAAGCTCA GGTTGCTGAA 
GATATTCTGG ACAAGTACCG GAATGCCATT AAACGGACCA GCCCCAGTGA AGGAGCAATG 
GCAAATGATG AGAGTGCAGA GGTCATGGGT GATGGTGAAA GTGCCCACGA CTCTCCTCGT 
GAGGAAGCAC TGCAGAACAT CTCAGCCGAC GACCTCCCGG ACTCCGCCAG CCAGGCAGCC 
CACCCTCAGG ACTCTGCTTT CTCTTACAGA GATGTAAAAA AGAAGCTGAG GCTCGCACTT 
TGCTCTGCAG ACTCTGTGGC CTTCCCAGTG CTAACTCATT CAACAAGGAA TGGATTGCCA 
GACCACACAG ACCCAGAAGA CAATGAAATT GTATGCTTCT TAAAAGTTCA AATAGCTGAA 
GCAATTAATT TACAAGATAA GAGCTTAATG GCTCAACTTC AAGAAACAAT GCGTTGTGTG 
TGCCGCTTTG ACAATAGGAC TTGTAGGAAG TTGCTGGCAT CCATTGCTGA AGACTACAGA 
AAAAGAGCTC CGTATATTGC TTATCTTACC CGCTGCCGAC AAGGACTGCA GACCACACAG 
GCTCACTTGG AAAGGCTCTT GCAAAGAGTT TTGCGGGACA AAGAGGTGGC CAATCGGTAC 
TTCACCACTG TCTGTGTGCG GTTACTGCTT GAGAGCAAAG AAAAGAAAAT CAGGGAGTTC 
ATTCAAGACT TTCAGAAACT TACTGCTGCT GATGATAAAA CGGCTCAGGT GGAGGATTTT 
CTACAGTTCC TGTATGGTGT GATGGCTCAG GATGTCATAT GGCAGAATGC CAGTGAGGAG 
CAGCTGCAGG ATGCCCAGCT GGCCATCGAG CGGAGTGTGA TGAATCGGAT CTTCAAGCTT 
GCTTTCTACC CTAACCAGGA TGGGGACATA CTTCGTGACC AGGTTCTGCA TGAACATATC 
CAGAGATTAT CTAAAGTAGT GACTGCAAAC CACAGAGCTC TGCAGATCCC TGAGGTTTAT 
CTCCGTGAGG CACCATGGCC ATCTGCACAG TCAGAAATCA GGACAATAAG TGCTTACAAA 
ACGCCCCGCG ACAAAGTGCA GTGCATCCTG AGGATGTGCT CCACCATCAT GAACCTCTTG 
AGCCTGGCCA ACGAGGACTC TGTCCCCGGG GCAGATGACT TTGTTCCTGT GTTGGTGTTT 
GTGTTAATAA AGGCAAACCC ACCCTGCCTG CTGTCCACTG TGCAGTACAT CAGCAGCTTC 
TATGCCAGCT GTCTGTCTGG AGAGGAGTCC TACTGGTGGA TGCAGTTCAC AGCAGCCGTG 
GAGTTTATTA AAACCATCGA TGACAGAAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAPVD1 ( NM_001356441.1 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_001343370.1 Mus musculus GTPase activating protein and VPS9 domains 1 (Gapvd1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu69990
Clone ID Related Accession (Same CDS sequence)	NM_001356441.1 , XM_011239148.2
Accession Version	NM_001356441.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4377bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	GTPase-activating protein and VPS9 domain-containing protein 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL929106.5 and AL845262.3. On Oct 4, 2017 this sequence version replaced XM_011239148.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## CDS exon combination :: SRR1660815.65908.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGAAGC TAGATATTCA CACATTGGCC CACCACCTCA AGCAGGAACG CTTATATGTG 
AGCTCTGAGA AACAGCTCAT TCAGAGGCTA AATGCCGATG TACTGAAGAC AGCTGAAAAG 
CTGTATCGAA CAGCGTGGAT TGCAAAGCAA CAGAGGATTA ATTTGGATCG TCTTATTATA 
ACCAGTGCTG AAGCTTCCCC TGCTGAGTGT TGCCAGCATG CCAAGATTTT GGAAGATACA 
CAGTTTGTTG ATGGATATAA GCAACTGGGA TTTCAGGAAA CAGCTTATGG AGAATTCCTG 
AGTCGGTTAA GAGAAAATCC ACGACTTATT GCCTCCTCTT TGGTTGCTGG AGAAAAACTC 
AATCAGGAGA ACACACAAAG TGTTATCTAC ACTGTTTTTA CCTCCTTGTA TGGCAACTGT 
ATTATGCAAG AAGATGAAAG CTACCTCCTT CAGGTTTTAC GATACTTGAT TGAATTTGAA 
CTTAAAGAAA GTGACAACCC CAGGCGCCTT TTGAGGAGAG GAACTTGTGC CTTCAGCATC 
TTATTTAAAC TTTTTTCTGA AGGACTGTTT TCTGCCAAAC TTTTCCTTAC GGCTACTTTG 
CATGAGCCAA TCATGCAGCT CCTCGTAGAG GATGAAGACC ACCTGGAAAC AGATCCAAAC 
AAGCTGATAG AGAGATTCTC TCCAGCTCAG CAAGAAAAAC TCTTTGGAGA GAAAGGCTCA 
GATAGATTTA GGCAAAAGGT TCAAGAAATG GTAGATTCCA ATGAGGCTAA ACTAGTGGCT 
TTGGTGAACA AATTTATTGG TTATCTTAAA CAGAATACAT ACTGCTTTCC ACATAGTTTG 
AGGTGGATTG TGTCACAGAT GTACAAAACT CTTTCTTGTG TAGACAGATT GGAGGTTGGG 
GAAGTCAGAG CAATGTGTAC CGATCTTCTG CTGGCTTGCT TCATTTGTCC TGCAGTTGTC 
AATCCAGAGC AGTATGGGAT AATATCTGAT GCTCCTATTA ATGAGGTGGC AAGATTTAAC 
CTGATGCAGG TTGGCCGCCT TTTGCAGCAG CTAGCAATGA CTGGCACTGA AGAAGGAGAT 
CCCCGGACCA AAAATAGCCT TGGAAAATTT GACAAAGGCT GTGTCGCTGC TTTCCTTGAT 
GTTGTAATTG GGGGCCGTGC AGTGGAGACT CCGCCAATGT CCTCTGTTAA TCTTCTGGAA 
GGCTTGAGCA GAACTGTGGT GTATATAAGT TACAGTCAGC TTATTACTCT GGTAAATTTT 
ATGAAGAGTG TGATGTCTGG AGATCAACTG AAAGAAGATA GAATGGCTCT TGACAATTTG 
TTGGCAAACC TGCCCCAGGC TAAGCCAGGG AAGAGCAGCA GTCTGGACAT GACTCCCTAT 
AGCACTCCTC AGATGTCTCC AGCAACCACT CCAGCCAACA AGAAGAATCG ATTACCTATA 
GCAACTCGGA GCAGAAGCCG AAGTAATATG TTAATGGACC TACATATGGA TCATGAAGGA 
TCATCTCAAG AAACCATCCA GGAGGTACAG CCAGAAGAGG TGCTGGTCAT TTCCTTAGGG 
ACAGGCCCTC AGCTCACTCC AGGGATGATG TCAGAAAATG AGGTTCTAAA CATGCAGCTT 
TCGGATGGAG GACAAGGAGA TGTCCCTGTT GATGAAAACA AACTCCACGG TAAACCTGAT 
AAAACCTTGC GCTTTTCCCT CTGCAGTGAT AATCTGGAAG GCATATCTGA AGGTCCTTCC 
AATCGCTCCA ATTCGGTGTC TTCTCTCGAC CTGGAGGGAG AGTCTGTATC TGAACTTGGG 
GCAGGACCTT CTGGGAGTAA CGGAGTTGAA GCACTGCAGC TGTTAGAGCA CGAGCAAGCT 
ACAACACAAG ATAATCTTGA TGATAAACTG AGGAAGTTTG AGATTCGTGA CATGATGGGA 
CTAACAGATG ACAGGGACAT ATCCGAGACT GTGAGTGAGA CCTGGAGCAC TGATGTCCTG 
GGAAGTGACT TCGACCCGAA CGTTGATGAA GATCGTTTGC AAGAGATTGC AGGTGCAGCA 
GCAGAGAACG TGTTAGGTAG TTTGCTGTGC CTTCCAGGTT CAGGGTCAGT TCTCCTTGAC 
CCTTGCACTG GCTCTACCAT ATCAGAGACC ACAAGTGAAG CTTGGAGTGT AGAGGTATTG 
CCAAGTGACT CAGAGGCTCC AGACTTAAAG CAGGAAGAGC GCCTCCAAGA GCTGGAGAGC 
TGTTCTGGAC TGGGCAGCAC ATCTGATGAT ACGGATGTCA GGGAGGTCAG TTCCCGCCCC 
AGCACACCAG GCCTCAGTGT TGTGTCCGGT ATAAGTGCTA CATCGGAGGA TATTCCCAAT 
AAGATCGAAG ACCTGAGATC TGAGTGCAGC TCTGATTTTG GGGGCAAAGA TTCTGTAACT 
AGTCCAGATA TGGATGACAT AGCCCATGGC GCTCATCAGC TGACCTCTCC TCCCTCTCAG 
TCAGAGTCTC TGCTTGCCAT GTTTGATCCA CTGTCCTCAC ATGAAGGGGC CTCTGCTGTG 
GTAAGGCCAA AGGTTCACTA TGCCCGACCC TCGCATCCAC CTCCGGATCC TCCAATCCTG 
GAAGGTGCAG TGGGAGGAAA TGAGGCCAGG TTGCCAAACT TTGGCTCTCA CGTTTTAACT 
GCAGCTGAAA TGGAGGCGTT CAAGCAGAGA CACTCATACC CTGAGAGATT GGTTCGCAGC 
AGAAGCTCTG ATATAGTGTC TTCTGTGCGG CGGCCCATGA GTGACCCCAG CTGGAACCGA 
CGTCCAGGGA ATGAAGAACT TCCTCCAGCT GCAGCCACTG GTGCTACTTC TTTGGTGGCT 
GCACCTCACT CGTCATCTTC GTCCCCGAGT AAGGACTCTT CTAGAGGAGA GACTGAGGAA 
CGCAAGGACA GTGACGATGA GAGGTCAGAC AGGAGCAGGC CTTGGTGGAG GAAGCGCTTT 
GTTTCAGCTA TGCCTAAAGC TCCTATACCA TTTAGAAAGA AAGAAAAACA AGAAAAAGAC 
AAAGATGATC TGGGTCCTGA CAGATTCTCA ACACTCACAG ATGAGCCCAG CCCTAGGCTC 
AGTGCTCAAG CTCAGGTTGC TGAAGATATT CTGGACAAGT ACCGGAATGC CATTAAACGG 
ACCAGCCCCA GTGAAGGAGC AATGGCAAAT GATGAGAGTG CAGAGGTCAT GGGTGATGGT 
GAAAGTGCCC ACGACTCTCC TCGTGAGGAA GCACTGCAGA ACATCTCAGC CGACGACCTC 
CCGGACTCCG CCAGCCAGGC AGCCCACCCT CAGGACTCTG CTTTCTCTTA CAGAGATGTA 
AAAAAGAAGC TGAGGCTCGC ACTTTGCTCT GCAGACTCTG TGGCCTTCCC AGTGCTAACT 
CATTCAACAA GGAATGGATT GCCAGACCAC ACAGACCCAG AAGACAATGA AATTGTATGC 
TTCTTAAAAG TTCAAATAGC TGAAGCAATT AATTTACAAG ATAAGAGCTT AATGGCTCAA 
CTTCAAGAAA CAATGCGTTG TGTGTGCCGC TTTGACAATA GGACTTGTAG GAAGTTGCTG 
GCATCCATTG CTGAAGACTA CAGAAAAAGA GCTCCGTATA TTGCTTATCT TACCCGCTGC 
CGACAAGGAC TGCAGACCAC ACAGGCTCAC TTGGAAAGGC TCTTGCAAAG AGTTTTGCGG 
GACAAAGAGG TGGCCAATCG GTACTTCACC ACTGTCTGTG TGCGGTTACT GCTTGAGAGC 
AAAGAAAAGA AAATCAGGGA GTTCATTCAA GACTTTCAGA AACTTACTGC TGCTGATGAT 
AAAACGGCTC AGGTGGAGGA TTTTCTACAG TTCCTGTATG GTGTGATGGC TCAGGATGTC 
ATATGGCAGA ATGCCAGTGA GGAGCAGCTG CAGGATGCCC AGCTGGCCAT CGAGCGGAGT 
GTGATGAATC GGATCTTCAA GCTTGCTTTC TACCCTAACC AGGATGGGGA CATACTTCGT 
GACCAGGTTC TGCATGAACA TATCCAGAGA TTATCTAAAG TAGTGACTGC AAACCACAGA 
GCTCTGCAGA TCCCTGAGGT TTATCTCCGT GAGGCACCAT GGCCATCTGC ACAGTCAGAA 
ATCAGGACAA TAAGTGCTTA CAAAACGCCC CGCGACAAAG TGCAGTGCAT CCTGAGGATG 
TGCTCCACCA TCATGAACCT CTTGAGCCTG GCCAACGAGG ACTCTGTCCC CGGGGCAGAT 
GACTTTGTTC CTGTGTTGGT GTTTGTGTTA ATAAAGGCAA ACCCACCCTG CCTGCTGTCC 
ACTGTGCAGT ACATCAGCAG CTTCTATGCC AGCTGTCTGT CTGGAGAGGA GTCCTACTGG 
TGGATGCAGT TCACAGCAGC CGTGGAGTTT ATTAAAACCA TCGATGACAG AAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001343370.1
CDS	583..4959
Translation	MVKLDIHTLAHHLKQERLYVSSEKQLIQRLNADVLKTAEKLYRTAWIAKQQRINLDRLIITSAEASPAECCQHAKILEDTQFVDGYKQLGFQETAYGEFLSRLRENPRLIASSLVAGEKLNQENTQSVIYTVFTSLYGNCIMQEDESYLLQVLRYLIEFELKESDNPRRLLRRGTCAFSILFKLFSEGLFSAKLFLTATLHEPIMQLLVEDEDHLETDPNKLIERFSPAQQEKLFGEKGSDRFRQKVQEMVDSNEAKLVALVNKFIGYLKQNTYCFPHSLRWIVSQMYKTLSCVDRLEVGEVRAMCTDLLLACFICPAVVNPEQYGIISDAPINEVARFNLMQVGRLLQQLAMTGTEEGDPRTKNSLGKFDKGCVAAFLDVVIGGRAVETPPMSSVNLLEGLSRTVVYISYSQLITLVNFMKSVMSGDQLKEDRMALDNLLANLPQAKPGKSSSLDMTPYSTPQMSPATTPANKKNRLPIATRSRSRSNMLMDLHMDHEGSSQETIQEVQPEEVLVISLGTGPQLTPGMMSENEVLNMQLSDGGQGDVPVDENKLHGKPDKTLRFSLCSDNLEGISEGPSNRSNSVSSLDLEGESVSELGAGPSGSNGVEALQLLEHEQATTQDNLDDKLRKFEIRDMMGLTDDRDISETVSETWSTDVLGSDFDPNVDEDRLQEIAGAAAENVLGSLLCLPGSGSVLLDPCTGSTISETTSEAWSVEVLPSDSEAPDLKQEERLQELESCSGLGSTSDDTDVREVSSRPSTPGLSVVSGISATSEDIPNKIEDLRSECSSDFGGKDSVTSPDMDDIAHGAHQLTSPPSQSESLLAMFDPLSSHEGASAVVRPKVHYARPSHPPPDPPILEGAVGGNEARLPNFGSHVLTAAEMEAFKQRHSYPERLVRSRSSDIVSSVRRPMSDPSWNRRPGNEELPPAAATGATSLVAAPHSSSSSPSKDSSRGETEERKDSDDERSDRSRPWWRKRFVSAMPKAPIPFRKKEKQEKDKDDLGPDRFSTLTDEPSPRLSAQAQVAEDILDKYRNAIKRTSPSEGAMANDESAEVMGDGESAHDSPREEALQNISADDLPDSASQAAHPQDSAFSYRDVKKKLRLALCSADSVAFPVLTHSTRNGLPDHTDPEDNEIVCFLKVQIAEAINLQDKSLMAQLQETMRCVCRFDNRTCRKLLASIAEDYRKRAPYIAYLTRCRQGLQTTQAHLERLLQRVLRDKEVANRYFTTVCVRLLLESKEKKIREFIQDFQKLTAADDKTAQVEDFLQFLYGVMAQDVIWQNASEEQLQDAQLAIERSVMNRIFKLAFYPNQDGDILRDQVLHEHIQRLSKVVTANHRALQIPEVYLREAPWPSAQSEIRTISAYKTPRDKVQCILRMCSTIMNLLSLANEDSVPGADDFVPVLVFVLIKANPPCLLSTVQYISSFYASCLSGEESYWWMQFTAAVEFIKTIDDRK

Target ORF information:

RefSeq Version	NM_001356441.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus GTPase activating protein and VPS9 domains 1 (Gapvd1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001356441.1

ORF Insert Sequence:

ATGGTGAAGC TAGATATTCA CACATTGGCC CACCACCTCA AGCAGGAACG CTTATATGTG 
AGCTCTGAGA AACAGCTCAT TCAGAGGCTA AATGCCGATG TACTGAAGAC AGCTGAAAAG 
CTGTATCGAA CAGCGTGGAT TGCAAAGCAA CAGAGGATTA ATTTGGATCG TCTTATTATA 
ACCAGTGCTG AAGCTTCCCC TGCTGAGTGT TGCCAGCATG CCAAGATTTT GGAAGATACA 
CAGTTTGTTG ATGGATATAA GCAACTGGGA TTTCAGGAAA CAGCTTATGG AGAATTCCTG 
AGTCGGTTAA GAGAAAATCC ACGACTTATT GCCTCCTCTT TGGTTGCTGG AGAAAAACTC 
AATCAGGAGA ACACACAAAG TGTTATCTAC ACTGTTTTTA CCTCCTTGTA TGGCAACTGT 
ATTATGCAAG AAGATGAAAG CTACCTCCTT CAGGTTTTAC GATACTTGAT TGAATTTGAA 
CTTAAAGAAA GTGACAACCC CAGGCGCCTT TTGAGGAGAG GAACTTGTGC CTTCAGCATC 
TTATTTAAAC TTTTTTCTGA AGGACTGTTT TCTGCCAAAC TTTTCCTTAC GGCTACTTTG 
CATGAGCCAA TCATGCAGCT CCTCGTAGAG GATGAAGACC ACCTGGAAAC AGATCCAAAC 
AAGCTGATAG AGAGATTCTC TCCAGCTCAG CAAGAAAAAC TCTTTGGAGA GAAAGGCTCA 
GATAGATTTA GGCAAAAGGT TCAAGAAATG GTAGATTCCA ATGAGGCTAA ACTAGTGGCT 
TTGGTGAACA AATTTATTGG TTATCTTAAA CAGAATACAT ACTGCTTTCC ACATAGTTTG 
AGGTGGATTG TGTCACAGAT GTACAAAACT CTTTCTTGTG TAGACAGATT GGAGGTTGGG 
GAAGTCAGAG CAATGTGTAC CGATCTTCTG CTGGCTTGCT TCATTTGTCC TGCAGTTGTC 
AATCCAGAGC AGTATGGGAT AATATCTGAT GCTCCTATTA ATGAGGTGGC AAGATTTAAC 
CTGATGCAGG TTGGCCGCCT TTTGCAGCAG CTAGCAATGA CTGGCACTGA AGAAGGAGAT 
CCCCGGACCA AAAATAGCCT TGGAAAATTT GACAAAGGCT GTGTCGCTGC TTTCCTTGAT 
GTTGTAATTG GGGGCCGTGC AGTGGAGACT CCGCCAATGT CCTCTGTTAA TCTTCTGGAA 
GGCTTGAGCA GAACTGTGGT GTATATAAGT TACAGTCAGC TTATTACTCT GGTAAATTTT 
ATGAAGAGTG TGATGTCTGG AGATCAACTG AAAGAAGATA GAATGGCTCT TGACAATTTG 
TTGGCAAACC TGCCCCAGGC TAAGCCAGGG AAGAGCAGCA GTCTGGACAT GACTCCCTAT 
AGCACTCCTC AGATGTCTCC AGCAACCACT CCAGCCAACA AGAAGAATCG ATTACCTATA 
GCAACTCGGA GCAGAAGCCG AAGTAATATG TTAATGGACC TACATATGGA TCATGAAGGA 
TCATCTCAAG AAACCATCCA GGAGGTACAG CCAGAAGAGG TGCTGGTCAT TTCCTTAGGG 
ACAGGCCCTC AGCTCACTCC AGGGATGATG TCAGAAAATG AGGTTCTAAA CATGCAGCTT 
TCGGATGGAG GACAAGGAGA TGTCCCTGTT GATGAAAACA AACTCCACGG TAAACCTGAT 
AAAACCTTGC GCTTTTCCCT CTGCAGTGAT AATCTGGAAG GCATATCTGA AGGTCCTTCC 
AATCGCTCCA ATTCGGTGTC TTCTCTCGAC CTGGAGGGAG AGTCTGTATC TGAACTTGGG 
GCAGGACCTT CTGGGAGTAA CGGAGTTGAA GCACTGCAGC TGTTAGAGCA CGAGCAAGCT 
ACAACACAAG ATAATCTTGA TGATAAACTG AGGAAGTTTG AGATTCGTGA CATGATGGGA 
CTAACAGATG ACAGGGACAT ATCCGAGACT GTGAGTGAGA CCTGGAGCAC TGATGTCCTG 
GGAAGTGACT TCGACCCGAA CGTTGATGAA GATCGTTTGC AAGAGATTGC AGGTGCAGCA 
GCAGAGAACG TGTTAGGTAG TTTGCTGTGC CTTCCAGGTT CAGGGTCAGT TCTCCTTGAC 
CCTTGCACTG GCTCTACCAT ATCAGAGACC ACAAGTGAAG CTTGGAGTGT AGAGGTATTG 
CCAAGTGACT CAGAGGCTCC AGACTTAAAG CAGGAAGAGC GCCTCCAAGA GCTGGAGAGC 
TGTTCTGGAC TGGGCAGCAC ATCTGATGAT ACGGATGTCA GGGAGGTCAG TTCCCGCCCC 
AGCACACCAG GCCTCAGTGT TGTGTCCGGT ATAAGTGCTA CATCGGAGGA TATTCCCAAT 
AAGATCGAAG ACCTGAGATC TGAGTGCAGC TCTGATTTTG GGGGCAAAGA TTCTGTAACT 
AGTCCAGATA TGGATGACAT AGCCCATGGC GCTCATCAGC TGACCTCTCC TCCCTCTCAG 
TCAGAGTCTC TGCTTGCCAT GTTTGATCCA CTGTCCTCAC ATGAAGGGGC CTCTGCTGTG 
GTAAGGCCAA AGGTTCACTA TGCCCGACCC TCGCATCCAC CTCCGGATCC TCCAATCCTG 
GAAGGTGCAG TGGGAGGAAA TGAGGCCAGG TTGCCAAACT TTGGCTCTCA CGTTTTAACT 
GCAGCTGAAA TGGAGGCGTT CAAGCAGAGA CACTCATACC CTGAGAGATT GGTTCGCAGC 
AGAAGCTCTG ATATAGTGTC TTCTGTGCGG CGGCCCATGA GTGACCCCAG CTGGAACCGA 
CGTCCAGGGA ATGAAGAACT TCCTCCAGCT GCAGCCACTG GTGCTACTTC TTTGGTGGCT 
GCACCTCACT CGTCATCTTC GTCCCCGAGT AAGGACTCTT CTAGAGGAGA GACTGAGGAA 
CGCAAGGACA GTGACGATGA GAGGTCAGAC AGGAGCAGGC CTTGGTGGAG GAAGCGCTTT 
GTTTCAGCTA TGCCTAAAGC TCCTATACCA TTTAGAAAGA AAGAAAAACA AGAAAAAGAC 
AAAGATGATC TGGGTCCTGA CAGATTCTCA ACACTCACAG ATGAGCCCAG CCCTAGGCTC 
AGTGCTCAAG CTCAGGTTGC TGAAGATATT CTGGACAAGT ACCGGAATGC CATTAAACGG 
ACCAGCCCCA GTGAAGGAGC AATGGCAAAT GATGAGAGTG CAGAGGTCAT GGGTGATGGT 
GAAAGTGCCC ACGACTCTCC TCGTGAGGAA GCACTGCAGA ACATCTCAGC CGACGACCTC 
CCGGACTCCG CCAGCCAGGC AGCCCACCCT CAGGACTCTG CTTTCTCTTA CAGAGATGTA 
AAAAAGAAGC TGAGGCTCGC ACTTTGCTCT GCAGACTCTG TGGCCTTCCC AGTGCTAACT 
CATTCAACAA GGAATGGATT GCCAGACCAC ACAGACCCAG AAGACAATGA AATTGTATGC 
TTCTTAAAAG TTCAAATAGC TGAAGCAATT AATTTACAAG ATAAGAGCTT AATGGCTCAA 
CTTCAAGAAA CAATGCGTTG TGTGTGCCGC TTTGACAATA GGACTTGTAG GAAGTTGCTG 
GCATCCATTG CTGAAGACTA CAGAAAAAGA GCTCCGTATA TTGCTTATCT TACCCGCTGC 
CGACAAGGAC TGCAGACCAC ACAGGCTCAC TTGGAAAGGC TCTTGCAAAG AGTTTTGCGG 
GACAAAGAGG TGGCCAATCG GTACTTCACC ACTGTCTGTG TGCGGTTACT GCTTGAGAGC 
AAAGAAAAGA AAATCAGGGA GTTCATTCAA GACTTTCAGA AACTTACTGC TGCTGATGAT 
AAAACGGCTC AGGTGGAGGA TTTTCTACAG TTCCTGTATG GTGTGATGGC TCAGGATGTC 
ATATGGCAGA ATGCCAGTGA GGAGCAGCTG CAGGATGCCC AGCTGGCCAT CGAGCGGAGT 
GTGATGAATC GGATCTTCAA GCTTGCTTTC TACCCTAACC AGGATGGGGA CATACTTCGT 
GACCAGGTTC TGCATGAACA TATCCAGAGA TTATCTAAAG TAGTGACTGC AAACCACAGA 
GCTCTGCAGA TCCCTGAGGT TTATCTCCGT GAGGCACCAT GGCCATCTGC ACAGTCAGAA 
ATCAGGACAA TAAGTGCTTA CAAAACGCCC CGCGACAAAG TGCAGTGCAT CCTGAGGATG 
TGCTCCACCA TCATGAACCT CTTGAGCCTG GCCAACGAGG ACTCTGTCCC CGGGGCAGAT 
GACTTTGTTC CTGTGTTGGT GTTTGTGTTA ATAAAGGCAA ACCCACCCTG CCTGCTGTCC 
ACTGTGCAGT ACATCAGCAG CTTCTATGCC AGCTGTCTGT CTGGAGAGGA GTCCTACTGG 
TGGATGCAGT TCACAGCAGC CGTGGAGTTT ATTAAAACCA TCGATGACAG AAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAPVD1 ( XM_011239145.1 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_011237447.1 Mus musculus GTPase activating protein and VPS9 domains 1 (Gapvd1), transcript variant X1, mRNA.

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CloneID	OMu69988
Clone ID Related Accession (Same CDS sequence)	XM_017319195.2 , XM_017319196.2 , XM_011239145.1 , XM_017319195.1 , XM_017319196.1
Accession Version	XM_011239145.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4458bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	GTPase-activating protein and VPS9 domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGC TAGATATTCA CACATTGGCC CACCACCTCA AGCAGGAACG CTTATATGTG 
AGCTCTGAGA AACAGCTCAT TCAGAGGCTA AATGCCGATG TACTGAAGAC AGCTGAAAAG 
CTGTATCGAA CAGCGTGGAT TGCAAAGCAA CAGAGGATTA ATTTGGATCG TCTTATTATA 
ACCAGTGCTG AAGCTTCCCC TGCTGAGTGT TGCCAGCATG CCAAGATTTT GGAAGATACA 
CAGTTTGTTG ATGGATATAA GCAACTGGGA TTTCAGGAAA CAGCTTATGG AGAATTCCTG 
AGTCGGTTAA GAGAAAATCC ACGACTTATT GCCTCCTCTT TGGTTGCTGG AGAAAAACTC 
AATCAGGAGA ACACACAAAG TGTTATCTAC ACTGTTTTTA CCTCCTTGTA TGGCAACTGT 
ATTATGCAAG AAGATGAAAG CTACCTCCTT CAGGTTTTAC GATACTTGAT TGAATTTGAA 
CTTAAAGAAA GTGACAACCC CAGGCGCCTT TTGAGGAGAG GAACTTGTGC CTTCAGCATC 
TTATTTAAAC TTTTTTCTGA AGGACTGTTT TCTGCCAAAC TTTTCCTTAC GGCTACTTTG 
CATGAGCCAA TCATGCAGCT CCTCGTAGAG GATGAAGACC ACCTGGAAAC AGATCCAAAC 
AAGCTGATAG AGAGATTCTC TCCAGCTCAG CAAGAAAAAC TCTTTGGAGA GAAAGGCTCA 
GATAGATTTA GGCAAAAGGT TCAAGAAATG GTAGATTCCA ATGAGGCTAA ACTAGTGGCT 
TTGGTGAACA AATTTATTGG TTATCTTAAA CAGAATACAT ACTGCTTTCC ACATAGTTTG 
AGGTGGATTG TGTCACAGAT GTACAAAACT CTTTCTTGTG TAGACAGATT GGAGGTTGGG 
GAAGTCAGAG CAATGTGTAC CGATCTTCTG CTGGCTTGCT TCATTTGTCC TGCAGTTGTC 
AATCCAGAGC AGTATGGGAT AATATCTGAT GCTCCTATTA ATGAGGTGGC AAGATTTAAC 
CTGATGCAGG TTGGCCGCCT TTTGCAGCAG CTAGCAATGA CTGGCACTGA AGAAGGAGAT 
CCCCGGACCA AAAATAGCCT TGGAAAATTT GACAAAGGCT GTGTCGCTGC TTTCCTTGAT 
GTTGTAATTG GGGGCCGTGC AGTGGAGACT CCGCCAATGT CCTCTGTTAA TCTTCTGGAA 
GGCTTGAGCA GAACTGTGGT GTATATAAGT TACAGTCAGC TTATTACTCT GGTAAATTTT 
ATGAAGAGTG TGATGTCTGG AGATCAACTG AAAGAAGATA GAATGGCTCT TGACAATTTG 
TTGGCAAACC TGCCCCAGGC TAAGCCAGGG AAGAGCAGCA GTCTGGACAT GACTCCCTAT 
AGCACTCCTC AGATGTCTCC AGCAACCACT CCAGCCAACA AGAAGAATCG ATTACCTATA 
GGACAACAGT TAGCAGCCAT CACTGCCTGG GATTCGTCAG CCACCAATCT TACTGCTCAT 
ATTCCTCTAG TAACCCCATT TGCAACTCGG AGCAGAAGCC GAAGTAATAT GTTAATGGAC 
CTACATATGG ATCATGAAGG ATCATCTCAA GAAACCATCC AGGAGGTACA GCCAGAAGAG 
GTGCTGGTCA TTTCCTTAGG GACAGGCCCT CAGCTCACTC CAGGGATGAT GTCAGAAAAT 
GAGGTTCTAA ACATGCAGCT TTCGGATGGA GGACAAGGAG ATGTCCCTGT TGATGAAAAC 
AAACTCCACG GTAAACCTGA TAAAACCTTG CGCTTTTCCC TCTGCAGTGA TAATCTGGAA 
GGCATATCTG AAGGTCCTTC CAATCGCTCC AATTCGGTGT CTTCTCTCGA CCTGGAGGGA 
GAGTCTGTAT CTGAACTTGG GGCAGGACCT TCTGGGAGTA ACGGAGTTGA AGCACTGCAG 
CTGTTAGAGC ACGAGCAAGC TACAACACAA GATAATCTTG ATGATAAACT GAGGAAGTTT 
GAGATTCGTG ACATGATGGG ACTAACAGAT GACAGGGACA TATCCGAGAC TGTGAGTGAG 
ACCTGGAGCA CTGATGTCCT GGGAAGTGAC TTCGACCCGA ACGTTGATGA AGATCGTTTG 
CAAGAGATTG CAGGTGCAGC AGCAGAGAAC GTGTTAGGTA GTTTGCTGTG CCTTCCAGGT 
TCAGGGTCAG TTCTCCTTGA CCCTTGCACT GGCTCTACCA TATCAGAGAC CACAAGTGAA 
GCTTGGAGTG TAGAGGTATT GCCAAGTGAC TCAGAGGCTC CAGACTTAAA GCAGGAAGAG 
CGCCTCCAAG AGCTGGAGAG CTGTTCTGGA CTGGGCAGCA CATCTGATGA TACGGATGTC 
AGGGAGGTCA GTTCCCGCCC CAGCACACCA GGCCTCAGTG TTGTGTCCGG TATAAGTGCT 
ACATCGGAGG ATATTCCCAA TAAGATCGAA GACCTGAGAT CTGAGTGCAG CTCTGATTTT 
GGGGGCAAAG ATTCTGTAAC TAGTCCAGAT ATGGATGACA TAGCCCATGG CGCTCATCAG 
CTGACCTCTC CTCCCTCTCA GTCAGAGTCT CTGCTTGCCA TGTTTGATCC ACTGTCCTCA 
CATGAAGGGG CCTCTGCTGT GGTAAGGCCA AAGGTTCACT ATGCCCGACC CTCGCATCCA 
CCTCCGGATC CTCCAATCCT GGAAGGTGCA GTGGGAGGAA ATGAGGCCAG GTTGCCAAAC 
TTTGGCTCTC ACGTTTTAAC TGCAGCTGAA ATGGAGGCGT TCAAGCAGAG ACACTCATAC 
CCTGAGAGAT TGGTTCGCAG CAGAAGCTCT GATATAGTGT CTTCTGTGCG GCGGCCCATG 
AGTGACCCCA GCTGGAACCG ACGTCCAGGG AATGAAGAAC TTCCTCCAGC TGCAGCCACT 
GGTGCTACTT CTTTGGTGGC TGCACCTCAC TCGTCATCTT CGTCCCCGAG TAAGGACTCT 
TCTAGAGGAG AGACTGAGGA ACGCAAGGAC AGTGACGATG AGAGGTCAGA CAGGAGCAGG 
CCTTGGTGGA GGAAGCGCTT TGTTTCAGCT ATGCCTAAAG CTCCTATACC ATTTAGAAAG 
AAAGAAAAAC AAGAAAAAGA CAAAGATGAT CTGGGTCCTG ACAGATTCTC AACACTCACA 
GATGAGCCCA GCCCTAGGCT CAGTGCTCAA GCTCAGGTTG CTGAAGATAT TCTGGACAAG 
TACCGGAATG CCATTAAACG GACCAGCCCC AGTGAAGGAG CAATGGCAAA TGATGAGAGT 
GCAGAGGTCA TGGGTGATGG TGAAAGTGCC CACGACTCTC CTCGTGAGGA AGCACTGCAG 
AACATCTCAG CCGACGACCT CCCGGACTCC GCCAGCCAGG CAGCCCACCC TCAGGACTCT 
GCTTTCTCTT ACAGAGATGT AAAAAAGAAG CTGAGGCTCG CACTTTGCTC TGCAGACTCT 
GTGGCCTTCC CAGTGCTAAC TCATTCAACA AGGAATGGAT TGCCAGACCA CACAGACCCA 
GAAGACAATG AAATTGTATG CTTCTTAAAA GTTCAAATAG CTGAAGCAAT TAATTTACAA 
GATAAGAGCT TAATGGCTCA ACTTCAAGAA ACAATGCGTT GTGTGTGCCG CTTTGACAAT 
AGGACTTGTA GGAAGTTGCT GGCATCCATT GCTGAAGACT ACAGAAAAAG AGCTCCGTAT 
ATTGCTTATC TTACCCGCTG CCGACAAGGA CTGCAGACCA CACAGGCTCA CTTGGAAAGG 
CTCTTGCAAA GAGTTTTGCG GGACAAAGAG GTGGCCAATC GGTACTTCAC CACTGTCTGT 
GTGCGGTTAC TGCTTGAGAG CAAAGAAAAG AAAATCAGGG AGTTCATTCA AGACTTTCAG 
AAACTTACTG CTGCTGATGA TAAAACGGCT CAGGTGGAGG ATTTTCTACA GTTCCTGTAT 
GGTGTGATGG CTCAGGATGT CATATGGCAG AATGCCAGTG AGGAGCAGCT GCAGGATGCC 
CAGCTGGCCA TCGAGCGGAG TGTGATGAAT CGGATCTTCA AGCTTGCTTT CTACCCTAAC 
CAGGATGGGG ACATACTTCG TGACCAGGTT CTGCATGAAC ATATCCAGAG ATTATCTAAA 
GTAGTGACTG CAAACCACAG AGCTCTGCAG ATCCCTGAGG TTTATCTCCG TGAGGCACCA 
TGGCCATCTG CACAGTCAGA AATCAGGACA ATAAGTGCTT ACAAAACGCC CCGCGACAAA 
GTGCAGTGCA TCCTGAGGAT GTGCTCCACC ATCATGAACC TCTTGAGCCT GGCCAACGAG 
GACTCTGTCC CCGGGGCAGA TGACTTTGTT CCTGTGTTGG TGTTTGTGTT AATAAAGGCA 
AACCCACCCT GCCTGCTGTC CACTGTGCAG TACATCAGCA GCTTCTATGC CAGCTGTCTG 
TCTGGAGAGG AGTCCTACTG GTGGATGCAG TTCACAGCAG CCGTGGAGTT TATTAAAACC 
ATCGATGACA GAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011237447.1
CDS	593..5050
Translation	MVKLDIHTLAHHLKQERLYVSSEKQLIQRLNADVLKTAEKLYRTAWIAKQQRINLDRLIITSAEASPAECCQHAKILEDTQFVDGYKQLGFQETAYGEFLSRLRENPRLIASSLVAGEKLNQENTQSVIYTVFTSLYGNCIMQEDESYLLQVLRYLIEFELKESDNPRRLLRRGTCAFSILFKLFSEGLFSAKLFLTATLHEPIMQLLVEDEDHLETDPNKLIERFSPAQQEKLFGEKGSDRFRQKVQEMVDSNEAKLVALVNKFIGYLKQNTYCFPHSLRWIVSQMYKTLSCVDRLEVGEVRAMCTDLLLACFICPAVVNPEQYGIISDAPINEVARFNLMQVGRLLQQLAMTGTEEGDPRTKNSLGKFDKGCVAAFLDVVIGGRAVETPPMSSVNLLEGLSRTVVYISYSQLITLVNFMKSVMSGDQLKEDRMALDNLLANLPQAKPGKSSSLDMTPYSTPQMSPATTPANKKNRLPIGQQLAAITAWDSSATNLTAHIPLVTPFATRSRSRSNMLMDLHMDHEGSSQETIQEVQPEEVLVISLGTGPQLTPGMMSENEVLNMQLSDGGQGDVPVDENKLHGKPDKTLRFSLCSDNLEGISEGPSNRSNSVSSLDLEGESVSELGAGPSGSNGVEALQLLEHEQATTQDNLDDKLRKFEIRDMMGLTDDRDISETVSETWSTDVLGSDFDPNVDEDRLQEIAGAAAENVLGSLLCLPGSGSVLLDPCTGSTISETTSEAWSVEVLPSDSEAPDLKQEERLQELESCSGLGSTSDDTDVREVSSRPSTPGLSVVSGISATSEDIPNKIEDLRSECSSDFGGKDSVTSPDMDDIAHGAHQLTSPPSQSESLLAMFDPLSSHEGASAVVRPKVHYARPSHPPPDPPILEGAVGGNEARLPNFGSHVLTAAEMEAFKQRHSYPERLVRSRSSDIVSSVRRPMSDPSWNRRPGNEELPPAAATGATSLVAAPHSSSSSPSKDSSRGETEERKDSDDERSDRSRPWWRKRFVSAMPKAPIPFRKKEKQEKDKDDLGPDRFSTLTDEPSPRLSAQAQVAEDILDKYRNAIKRTSPSEGAMANDESAEVMGDGESAHDSPREEALQNISADDLPDSASQAAHPQDSAFSYRDVKKKLRLALCSADSVAFPVLTHSTRNGLPDHTDPEDNEIVCFLKVQIAEAINLQDKSLMAQLQETMRCVCRFDNRTCRKLLASIAEDYRKRAPYIAYLTRCRQGLQTTQAHLERLLQRVLRDKEVANRYFTTVCVRLLLESKEKKIREFIQDFQKLTAADDKTAQVEDFLQFLYGVMAQDVIWQNASEEQLQDAQLAIERSVMNRIFKLAFYPNQDGDILRDQVLHEHIQRLSKVVTANHRALQIPEVYLREAPWPSAQSEIRTISAYKTPRDKVQCILRMCSTIMNLLSLANEDSVPGADDFVPVLVFVLIKANPPCLLSTVQYISSFYASCLSGEESYWWMQFTAAVEFIKTIDDRK

Target ORF information:

RefSeq Version	XM_011239145.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus GTPase activating protein and VPS9 domains 1 (Gapvd1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011239145.1

ORF Insert Sequence:

ATGGTGAAGC TAGATATTCA CACATTGGCC CACCACCTCA AGCAGGAACG CTTATATGTG 
AGCTCTGAGA AACAGCTCAT TCAGAGGCTA AATGCCGATG TACTGAAGAC AGCTGAAAAG 
CTGTATCGAA CAGCGTGGAT TGCAAAGCAA CAGAGGATTA ATTTGGATCG TCTTATTATA 
ACCAGTGCTG AAGCTTCCCC TGCTGAGTGT TGCCAGCATG CCAAGATTTT GGAAGATACA 
CAGTTTGTTG ATGGATATAA GCAACTGGGA TTTCAGGAAA CAGCTTATGG AGAATTCCTG 
AGTCGGTTAA GAGAAAATCC ACGACTTATT GCCTCCTCTT TGGTTGCTGG AGAAAAACTC 
AATCAGGAGA ACACACAAAG TGTTATCTAC ACTGTTTTTA CCTCCTTGTA TGGCAACTGT 
ATTATGCAAG AAGATGAAAG CTACCTCCTT CAGGTTTTAC GATACTTGAT TGAATTTGAA 
CTTAAAGAAA GTGACAACCC CAGGCGCCTT TTGAGGAGAG GAACTTGTGC CTTCAGCATC 
TTATTTAAAC TTTTTTCTGA AGGACTGTTT TCTGCCAAAC TTTTCCTTAC GGCTACTTTG 
CATGAGCCAA TCATGCAGCT CCTCGTAGAG GATGAAGACC ACCTGGAAAC AGATCCAAAC 
AAGCTGATAG AGAGATTCTC TCCAGCTCAG CAAGAAAAAC TCTTTGGAGA GAAAGGCTCA 
GATAGATTTA GGCAAAAGGT TCAAGAAATG GTAGATTCCA ATGAGGCTAA ACTAGTGGCT 
TTGGTGAACA AATTTATTGG TTATCTTAAA CAGAATACAT ACTGCTTTCC ACATAGTTTG 
AGGTGGATTG TGTCACAGAT GTACAAAACT CTTTCTTGTG TAGACAGATT GGAGGTTGGG 
GAAGTCAGAG CAATGTGTAC CGATCTTCTG CTGGCTTGCT TCATTTGTCC TGCAGTTGTC 
AATCCAGAGC AGTATGGGAT AATATCTGAT GCTCCTATTA ATGAGGTGGC AAGATTTAAC 
CTGATGCAGG TTGGCCGCCT TTTGCAGCAG CTAGCAATGA CTGGCACTGA AGAAGGAGAT 
CCCCGGACCA AAAATAGCCT TGGAAAATTT GACAAAGGCT GTGTCGCTGC TTTCCTTGAT 
GTTGTAATTG GGGGCCGTGC AGTGGAGACT CCGCCAATGT CCTCTGTTAA TCTTCTGGAA 
GGCTTGAGCA GAACTGTGGT GTATATAAGT TACAGTCAGC TTATTACTCT GGTAAATTTT 
ATGAAGAGTG TGATGTCTGG AGATCAACTG AAAGAAGATA GAATGGCTCT TGACAATTTG 
TTGGCAAACC TGCCCCAGGC TAAGCCAGGG AAGAGCAGCA GTCTGGACAT GACTCCCTAT 
AGCACTCCTC AGATGTCTCC AGCAACCACT CCAGCCAACA AGAAGAATCG ATTACCTATA 
GGACAACAGT TAGCAGCCAT CACTGCCTGG GATTCGTCAG CCACCAATCT TACTGCTCAT 
ATTCCTCTAG TAACCCCATT TGCAACTCGG AGCAGAAGCC GAAGTAATAT GTTAATGGAC 
CTACATATGG ATCATGAAGG ATCATCTCAA GAAACCATCC AGGAGGTACA GCCAGAAGAG 
GTGCTGGTCA TTTCCTTAGG GACAGGCCCT CAGCTCACTC CAGGGATGAT GTCAGAAAAT 
GAGGTTCTAA ACATGCAGCT TTCGGATGGA GGACAAGGAG ATGTCCCTGT TGATGAAAAC 
AAACTCCACG GTAAACCTGA TAAAACCTTG CGCTTTTCCC TCTGCAGTGA TAATCTGGAA 
GGCATATCTG AAGGTCCTTC CAATCGCTCC AATTCGGTGT CTTCTCTCGA CCTGGAGGGA 
GAGTCTGTAT CTGAACTTGG GGCAGGACCT TCTGGGAGTA ACGGAGTTGA AGCACTGCAG 
CTGTTAGAGC ACGAGCAAGC TACAACACAA GATAATCTTG ATGATAAACT GAGGAAGTTT 
GAGATTCGTG ACATGATGGG ACTAACAGAT GACAGGGACA TATCCGAGAC TGTGAGTGAG 
ACCTGGAGCA CTGATGTCCT GGGAAGTGAC TTCGACCCGA ACGTTGATGA AGATCGTTTG 
CAAGAGATTG CAGGTGCAGC AGCAGAGAAC GTGTTAGGTA GTTTGCTGTG CCTTCCAGGT 
TCAGGGTCAG TTCTCCTTGA CCCTTGCACT GGCTCTACCA TATCAGAGAC CACAAGTGAA 
GCTTGGAGTG TAGAGGTATT GCCAAGTGAC TCAGAGGCTC CAGACTTAAA GCAGGAAGAG 
CGCCTCCAAG AGCTGGAGAG CTGTTCTGGA CTGGGCAGCA CATCTGATGA TACGGATGTC 
AGGGAGGTCA GTTCCCGCCC CAGCACACCA GGCCTCAGTG TTGTGTCCGG TATAAGTGCT 
ACATCGGAGG ATATTCCCAA TAAGATCGAA GACCTGAGAT CTGAGTGCAG CTCTGATTTT 
GGGGGCAAAG ATTCTGTAAC TAGTCCAGAT ATGGATGACA TAGCCCATGG CGCTCATCAG 
CTGACCTCTC CTCCCTCTCA GTCAGAGTCT CTGCTTGCCA TGTTTGATCC ACTGTCCTCA 
CATGAAGGGG CCTCTGCTGT GGTAAGGCCA AAGGTTCACT ATGCCCGACC CTCGCATCCA 
CCTCCGGATC CTCCAATCCT GGAAGGTGCA GTGGGAGGAA ATGAGGCCAG GTTGCCAAAC 
TTTGGCTCTC ACGTTTTAAC TGCAGCTGAA ATGGAGGCGT TCAAGCAGAG ACACTCATAC 
CCTGAGAGAT TGGTTCGCAG CAGAAGCTCT GATATAGTGT CTTCTGTGCG GCGGCCCATG 
AGTGACCCCA GCTGGAACCG ACGTCCAGGG AATGAAGAAC TTCCTCCAGC TGCAGCCACT 
GGTGCTACTT CTTTGGTGGC TGCACCTCAC TCGTCATCTT CGTCCCCGAG TAAGGACTCT 
TCTAGAGGAG AGACTGAGGA ACGCAAGGAC AGTGACGATG AGAGGTCAGA CAGGAGCAGG 
CCTTGGTGGA GGAAGCGCTT TGTTTCAGCT ATGCCTAAAG CTCCTATACC ATTTAGAAAG 
AAAGAAAAAC AAGAAAAAGA CAAAGATGAT CTGGGTCCTG ACAGATTCTC AACACTCACA 
GATGAGCCCA GCCCTAGGCT CAGTGCTCAA GCTCAGGTTG CTGAAGATAT TCTGGACAAG 
TACCGGAATG CCATTAAACG GACCAGCCCC AGTGAAGGAG CAATGGCAAA TGATGAGAGT 
GCAGAGGTCA TGGGTGATGG TGAAAGTGCC CACGACTCTC CTCGTGAGGA AGCACTGCAG 
AACATCTCAG CCGACGACCT CCCGGACTCC GCCAGCCAGG CAGCCCACCC TCAGGACTCT 
GCTTTCTCTT ACAGAGATGT AAAAAAGAAG CTGAGGCTCG CACTTTGCTC TGCAGACTCT 
GTGGCCTTCC CAGTGCTAAC TCATTCAACA AGGAATGGAT TGCCAGACCA CACAGACCCA 
GAAGACAATG AAATTGTATG CTTCTTAAAA GTTCAAATAG CTGAAGCAAT TAATTTACAA 
GATAAGAGCT TAATGGCTCA ACTTCAAGAA ACAATGCGTT GTGTGTGCCG CTTTGACAAT 
AGGACTTGTA GGAAGTTGCT GGCATCCATT GCTGAAGACT ACAGAAAAAG AGCTCCGTAT 
ATTGCTTATC TTACCCGCTG CCGACAAGGA CTGCAGACCA CACAGGCTCA CTTGGAAAGG 
CTCTTGCAAA GAGTTTTGCG GGACAAAGAG GTGGCCAATC GGTACTTCAC CACTGTCTGT 
GTGCGGTTAC TGCTTGAGAG CAAAGAAAAG AAAATCAGGG AGTTCATTCA AGACTTTCAG 
AAACTTACTG CTGCTGATGA TAAAACGGCT CAGGTGGAGG ATTTTCTACA GTTCCTGTAT 
GGTGTGATGG CTCAGGATGT CATATGGCAG AATGCCAGTG AGGAGCAGCT GCAGGATGCC 
CAGCTGGCCA TCGAGCGGAG TGTGATGAAT CGGATCTTCA AGCTTGCTTT CTACCCTAAC 
CAGGATGGGG ACATACTTCG TGACCAGGTT CTGCATGAAC ATATCCAGAG ATTATCTAAA 
GTAGTGACTG CAAACCACAG AGCTCTGCAG ATCCCTGAGG TTTATCTCCG TGAGGCACCA 
TGGCCATCTG CACAGTCAGA AATCAGGACA ATAAGTGCTT ACAAAACGCC CCGCGACAAA 
GTGCAGTGCA TCCTGAGGAT GTGCTCCACC ATCATGAACC TCTTGAGCCT GGCCAACGAG 
GACTCTGTCC CCGGGGCAGA TGACTTTGTT CCTGTGTTGG TGTTTGTGTT AATAAAGGCA 
AACCCACCCT GCCTGCTGTC CACTGTGCAG TACATCAGCA GCTTCTATGC CAGCTGTCTG 
TCTGGAGAGG AGTCCTACTG GTGGATGCAG TTCACAGCAG CCGTGGAGTT TATTAAAACC 
ATCGATGACA GAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAPVD1 ( XM_017319195.1 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_017174684.1 Mus musculus GTPase activating protein and VPS9 domains 1 (Gapvd1), transcript variant X2, mRNA.

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CloneID	OMu69988
Clone ID Related Accession (Same CDS sequence)	XM_017319195.2 , XM_017319196.2 , XM_011239145.1 , XM_017319195.1 , XM_017319196.1
Accession Version	XM_017319195.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4458bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	GTPase-activating protein and VPS9 domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGC TAGATATTCA CACATTGGCC CACCACCTCA AGCAGGAACG CTTATATGTG 
AGCTCTGAGA AACAGCTCAT TCAGAGGCTA AATGCCGATG TACTGAAGAC AGCTGAAAAG 
CTGTATCGAA CAGCGTGGAT TGCAAAGCAA CAGAGGATTA ATTTGGATCG TCTTATTATA 
ACCAGTGCTG AAGCTTCCCC TGCTGAGTGT TGCCAGCATG CCAAGATTTT GGAAGATACA 
CAGTTTGTTG ATGGATATAA GCAACTGGGA TTTCAGGAAA CAGCTTATGG AGAATTCCTG 
AGTCGGTTAA GAGAAAATCC ACGACTTATT GCCTCCTCTT TGGTTGCTGG AGAAAAACTC 
AATCAGGAGA ACACACAAAG TGTTATCTAC ACTGTTTTTA CCTCCTTGTA TGGCAACTGT 
ATTATGCAAG AAGATGAAAG CTACCTCCTT CAGGTTTTAC GATACTTGAT TGAATTTGAA 
CTTAAAGAAA GTGACAACCC CAGGCGCCTT TTGAGGAGAG GAACTTGTGC CTTCAGCATC 
TTATTTAAAC TTTTTTCTGA AGGACTGTTT TCTGCCAAAC TTTTCCTTAC GGCTACTTTG 
CATGAGCCAA TCATGCAGCT CCTCGTAGAG GATGAAGACC ACCTGGAAAC AGATCCAAAC 
AAGCTGATAG AGAGATTCTC TCCAGCTCAG CAAGAAAAAC TCTTTGGAGA GAAAGGCTCA 
GATAGATTTA GGCAAAAGGT TCAAGAAATG GTAGATTCCA ATGAGGCTAA ACTAGTGGCT 
TTGGTGAACA AATTTATTGG TTATCTTAAA CAGAATACAT ACTGCTTTCC ACATAGTTTG 
AGGTGGATTG TGTCACAGAT GTACAAAACT CTTTCTTGTG TAGACAGATT GGAGGTTGGG 
GAAGTCAGAG CAATGTGTAC CGATCTTCTG CTGGCTTGCT TCATTTGTCC TGCAGTTGTC 
AATCCAGAGC AGTATGGGAT AATATCTGAT GCTCCTATTA ATGAGGTGGC AAGATTTAAC 
CTGATGCAGG TTGGCCGCCT TTTGCAGCAG CTAGCAATGA CTGGCACTGA AGAAGGAGAT 
CCCCGGACCA AAAATAGCCT TGGAAAATTT GACAAAGGCT GTGTCGCTGC TTTCCTTGAT 
GTTGTAATTG GGGGCCGTGC AGTGGAGACT CCGCCAATGT CCTCTGTTAA TCTTCTGGAA 
GGCTTGAGCA GAACTGTGGT GTATATAAGT TACAGTCAGC TTATTACTCT GGTAAATTTT 
ATGAAGAGTG TGATGTCTGG AGATCAACTG AAAGAAGATA GAATGGCTCT TGACAATTTG 
TTGGCAAACC TGCCCCAGGC TAAGCCAGGG AAGAGCAGCA GTCTGGACAT GACTCCCTAT 
AGCACTCCTC AGATGTCTCC AGCAACCACT CCAGCCAACA AGAAGAATCG ATTACCTATA 
GGACAACAGT TAGCAGCCAT CACTGCCTGG GATTCGTCAG CCACCAATCT TACTGCTCAT 
ATTCCTCTAG TAACCCCATT TGCAACTCGG AGCAGAAGCC GAAGTAATAT GTTAATGGAC 
CTACATATGG ATCATGAAGG ATCATCTCAA GAAACCATCC AGGAGGTACA GCCAGAAGAG 
GTGCTGGTCA TTTCCTTAGG GACAGGCCCT CAGCTCACTC CAGGGATGAT GTCAGAAAAT 
GAGGTTCTAA ACATGCAGCT TTCGGATGGA GGACAAGGAG ATGTCCCTGT TGATGAAAAC 
AAACTCCACG GTAAACCTGA TAAAACCTTG CGCTTTTCCC TCTGCAGTGA TAATCTGGAA 
GGCATATCTG AAGGTCCTTC CAATCGCTCC AATTCGGTGT CTTCTCTCGA CCTGGAGGGA 
GAGTCTGTAT CTGAACTTGG GGCAGGACCT TCTGGGAGTA ACGGAGTTGA AGCACTGCAG 
CTGTTAGAGC ACGAGCAAGC TACAACACAA GATAATCTTG ATGATAAACT GAGGAAGTTT 
GAGATTCGTG ACATGATGGG ACTAACAGAT GACAGGGACA TATCCGAGAC TGTGAGTGAG 
ACCTGGAGCA CTGATGTCCT GGGAAGTGAC TTCGACCCGA ACGTTGATGA AGATCGTTTG 
CAAGAGATTG CAGGTGCAGC AGCAGAGAAC GTGTTAGGTA GTTTGCTGTG CCTTCCAGGT 
TCAGGGTCAG TTCTCCTTGA CCCTTGCACT GGCTCTACCA TATCAGAGAC CACAAGTGAA 
GCTTGGAGTG TAGAGGTATT GCCAAGTGAC TCAGAGGCTC CAGACTTAAA GCAGGAAGAG 
CGCCTCCAAG AGCTGGAGAG CTGTTCTGGA CTGGGCAGCA CATCTGATGA TACGGATGTC 
AGGGAGGTCA GTTCCCGCCC CAGCACACCA GGCCTCAGTG TTGTGTCCGG TATAAGTGCT 
ACATCGGAGG ATATTCCCAA TAAGATCGAA GACCTGAGAT CTGAGTGCAG CTCTGATTTT 
GGGGGCAAAG ATTCTGTAAC TAGTCCAGAT ATGGATGACA TAGCCCATGG CGCTCATCAG 
CTGACCTCTC CTCCCTCTCA GTCAGAGTCT CTGCTTGCCA TGTTTGATCC ACTGTCCTCA 
CATGAAGGGG CCTCTGCTGT GGTAAGGCCA AAGGTTCACT ATGCCCGACC CTCGCATCCA 
CCTCCGGATC CTCCAATCCT GGAAGGTGCA GTGGGAGGAA ATGAGGCCAG GTTGCCAAAC 
TTTGGCTCTC ACGTTTTAAC TGCAGCTGAA ATGGAGGCGT TCAAGCAGAG ACACTCATAC 
CCTGAGAGAT TGGTTCGCAG CAGAAGCTCT GATATAGTGT CTTCTGTGCG GCGGCCCATG 
AGTGACCCCA GCTGGAACCG ACGTCCAGGG AATGAAGAAC TTCCTCCAGC TGCAGCCACT 
GGTGCTACTT CTTTGGTGGC TGCACCTCAC TCGTCATCTT CGTCCCCGAG TAAGGACTCT 
TCTAGAGGAG AGACTGAGGA ACGCAAGGAC AGTGACGATG AGAGGTCAGA CAGGAGCAGG 
CCTTGGTGGA GGAAGCGCTT TGTTTCAGCT ATGCCTAAAG CTCCTATACC ATTTAGAAAG 
AAAGAAAAAC AAGAAAAAGA CAAAGATGAT CTGGGTCCTG ACAGATTCTC AACACTCACA 
GATGAGCCCA GCCCTAGGCT CAGTGCTCAA GCTCAGGTTG CTGAAGATAT TCTGGACAAG 
TACCGGAATG CCATTAAACG GACCAGCCCC AGTGAAGGAG CAATGGCAAA TGATGAGAGT 
GCAGAGGTCA TGGGTGATGG TGAAAGTGCC CACGACTCTC CTCGTGAGGA AGCACTGCAG 
AACATCTCAG CCGACGACCT CCCGGACTCC GCCAGCCAGG CAGCCCACCC TCAGGACTCT 
GCTTTCTCTT ACAGAGATGT AAAAAAGAAG CTGAGGCTCG CACTTTGCTC TGCAGACTCT 
GTGGCCTTCC CAGTGCTAAC TCATTCAACA AGGAATGGAT TGCCAGACCA CACAGACCCA 
GAAGACAATG AAATTGTATG CTTCTTAAAA GTTCAAATAG CTGAAGCAAT TAATTTACAA 
GATAAGAGCT TAATGGCTCA ACTTCAAGAA ACAATGCGTT GTGTGTGCCG CTTTGACAAT 
AGGACTTGTA GGAAGTTGCT GGCATCCATT GCTGAAGACT ACAGAAAAAG AGCTCCGTAT 
ATTGCTTATC TTACCCGCTG CCGACAAGGA CTGCAGACCA CACAGGCTCA CTTGGAAAGG 
CTCTTGCAAA GAGTTTTGCG GGACAAAGAG GTGGCCAATC GGTACTTCAC CACTGTCTGT 
GTGCGGTTAC TGCTTGAGAG CAAAGAAAAG AAAATCAGGG AGTTCATTCA AGACTTTCAG 
AAACTTACTG CTGCTGATGA TAAAACGGCT CAGGTGGAGG ATTTTCTACA GTTCCTGTAT 
GGTGTGATGG CTCAGGATGT CATATGGCAG AATGCCAGTG AGGAGCAGCT GCAGGATGCC 
CAGCTGGCCA TCGAGCGGAG TGTGATGAAT CGGATCTTCA AGCTTGCTTT CTACCCTAAC 
CAGGATGGGG ACATACTTCG TGACCAGGTT CTGCATGAAC ATATCCAGAG ATTATCTAAA 
GTAGTGACTG CAAACCACAG AGCTCTGCAG ATCCCTGAGG TTTATCTCCG TGAGGCACCA 
TGGCCATCTG CACAGTCAGA AATCAGGACA ATAAGTGCTT ACAAAACGCC CCGCGACAAA 
GTGCAGTGCA TCCTGAGGAT GTGCTCCACC ATCATGAACC TCTTGAGCCT GGCCAACGAG 
GACTCTGTCC CCGGGGCAGA TGACTTTGTT CCTGTGTTGG TGTTTGTGTT AATAAAGGCA 
AACCCACCCT GCCTGCTGTC CACTGTGCAG TACATCAGCA GCTTCTATGC CAGCTGTCTG 
TCTGGAGAGG AGTCCTACTG GTGGATGCAG TTCACAGCAG CCGTGGAGTT TATTAAAACC 
ATCGATGACA GAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017174684.1
CDS	431..4888
Translation	MVKLDIHTLAHHLKQERLYVSSEKQLIQRLNADVLKTAEKLYRTAWIAKQQRINLDRLIITSAEASPAECCQHAKILEDTQFVDGYKQLGFQETAYGEFLSRLRENPRLIASSLVAGEKLNQENTQSVIYTVFTSLYGNCIMQEDESYLLQVLRYLIEFELKESDNPRRLLRRGTCAFSILFKLFSEGLFSAKLFLTATLHEPIMQLLVEDEDHLETDPNKLIERFSPAQQEKLFGEKGSDRFRQKVQEMVDSNEAKLVALVNKFIGYLKQNTYCFPHSLRWIVSQMYKTLSCVDRLEVGEVRAMCTDLLLACFICPAVVNPEQYGIISDAPINEVARFNLMQVGRLLQQLAMTGTEEGDPRTKNSLGKFDKGCVAAFLDVVIGGRAVETPPMSSVNLLEGLSRTVVYISYSQLITLVNFMKSVMSGDQLKEDRMALDNLLANLPQAKPGKSSSLDMTPYSTPQMSPATTPANKKNRLPIGQQLAAITAWDSSATNLTAHIPLVTPFATRSRSRSNMLMDLHMDHEGSSQETIQEVQPEEVLVISLGTGPQLTPGMMSENEVLNMQLSDGGQGDVPVDENKLHGKPDKTLRFSLCSDNLEGISEGPSNRSNSVSSLDLEGESVSELGAGPSGSNGVEALQLLEHEQATTQDNLDDKLRKFEIRDMMGLTDDRDISETVSETWSTDVLGSDFDPNVDEDRLQEIAGAAAENVLGSLLCLPGSGSVLLDPCTGSTISETTSEAWSVEVLPSDSEAPDLKQEERLQELESCSGLGSTSDDTDVREVSSRPSTPGLSVVSGISATSEDIPNKIEDLRSECSSDFGGKDSVTSPDMDDIAHGAHQLTSPPSQSESLLAMFDPLSSHEGASAVVRPKVHYARPSHPPPDPPILEGAVGGNEARLPNFGSHVLTAAEMEAFKQRHSYPERLVRSRSSDIVSSVRRPMSDPSWNRRPGNEELPPAAATGATSLVAAPHSSSSSPSKDSSRGETEERKDSDDERSDRSRPWWRKRFVSAMPKAPIPFRKKEKQEKDKDDLGPDRFSTLTDEPSPRLSAQAQVAEDILDKYRNAIKRTSPSEGAMANDESAEVMGDGESAHDSPREEALQNISADDLPDSASQAAHPQDSAFSYRDVKKKLRLALCSADSVAFPVLTHSTRNGLPDHTDPEDNEIVCFLKVQIAEAINLQDKSLMAQLQETMRCVCRFDNRTCRKLLASIAEDYRKRAPYIAYLTRCRQGLQTTQAHLERLLQRVLRDKEVANRYFTTVCVRLLLESKEKKIREFIQDFQKLTAADDKTAQVEDFLQFLYGVMAQDVIWQNASEEQLQDAQLAIERSVMNRIFKLAFYPNQDGDILRDQVLHEHIQRLSKVVTANHRALQIPEVYLREAPWPSAQSEIRTISAYKTPRDKVQCILRMCSTIMNLLSLANEDSVPGADDFVPVLVFVLIKANPPCLLSTVQYISSFYASCLSGEESYWWMQFTAAVEFIKTIDDRK

Target ORF information:

RefSeq Version	XM_017319195.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus GTPase activating protein and VPS9 domains 1 (Gapvd1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319195.1

ORF Insert Sequence:

ATGGTGAAGC TAGATATTCA CACATTGGCC CACCACCTCA AGCAGGAACG CTTATATGTG 
AGCTCTGAGA AACAGCTCAT TCAGAGGCTA AATGCCGATG TACTGAAGAC AGCTGAAAAG 
CTGTATCGAA CAGCGTGGAT TGCAAAGCAA CAGAGGATTA ATTTGGATCG TCTTATTATA 
ACCAGTGCTG AAGCTTCCCC TGCTGAGTGT TGCCAGCATG CCAAGATTTT GGAAGATACA 
CAGTTTGTTG ATGGATATAA GCAACTGGGA TTTCAGGAAA CAGCTTATGG AGAATTCCTG 
AGTCGGTTAA GAGAAAATCC ACGACTTATT GCCTCCTCTT TGGTTGCTGG AGAAAAACTC 
AATCAGGAGA ACACACAAAG TGTTATCTAC ACTGTTTTTA CCTCCTTGTA TGGCAACTGT 
ATTATGCAAG AAGATGAAAG CTACCTCCTT CAGGTTTTAC GATACTTGAT TGAATTTGAA 
CTTAAAGAAA GTGACAACCC CAGGCGCCTT TTGAGGAGAG GAACTTGTGC CTTCAGCATC 
TTATTTAAAC TTTTTTCTGA AGGACTGTTT TCTGCCAAAC TTTTCCTTAC GGCTACTTTG 
CATGAGCCAA TCATGCAGCT CCTCGTAGAG GATGAAGACC ACCTGGAAAC AGATCCAAAC 
AAGCTGATAG AGAGATTCTC TCCAGCTCAG CAAGAAAAAC TCTTTGGAGA GAAAGGCTCA 
GATAGATTTA GGCAAAAGGT TCAAGAAATG GTAGATTCCA ATGAGGCTAA ACTAGTGGCT 
TTGGTGAACA AATTTATTGG TTATCTTAAA CAGAATACAT ACTGCTTTCC ACATAGTTTG 
AGGTGGATTG TGTCACAGAT GTACAAAACT CTTTCTTGTG TAGACAGATT GGAGGTTGGG 
GAAGTCAGAG CAATGTGTAC CGATCTTCTG CTGGCTTGCT TCATTTGTCC TGCAGTTGTC 
AATCCAGAGC AGTATGGGAT AATATCTGAT GCTCCTATTA ATGAGGTGGC AAGATTTAAC 
CTGATGCAGG TTGGCCGCCT TTTGCAGCAG CTAGCAATGA CTGGCACTGA AGAAGGAGAT 
CCCCGGACCA AAAATAGCCT TGGAAAATTT GACAAAGGCT GTGTCGCTGC TTTCCTTGAT 
GTTGTAATTG GGGGCCGTGC AGTGGAGACT CCGCCAATGT CCTCTGTTAA TCTTCTGGAA 
GGCTTGAGCA GAACTGTGGT GTATATAAGT TACAGTCAGC TTATTACTCT GGTAAATTTT 
ATGAAGAGTG TGATGTCTGG AGATCAACTG AAAGAAGATA GAATGGCTCT TGACAATTTG 
TTGGCAAACC TGCCCCAGGC TAAGCCAGGG AAGAGCAGCA GTCTGGACAT GACTCCCTAT 
AGCACTCCTC AGATGTCTCC AGCAACCACT CCAGCCAACA AGAAGAATCG ATTACCTATA 
GGACAACAGT TAGCAGCCAT CACTGCCTGG GATTCGTCAG CCACCAATCT TACTGCTCAT 
ATTCCTCTAG TAACCCCATT TGCAACTCGG AGCAGAAGCC GAAGTAATAT GTTAATGGAC 
CTACATATGG ATCATGAAGG ATCATCTCAA GAAACCATCC AGGAGGTACA GCCAGAAGAG 
GTGCTGGTCA TTTCCTTAGG GACAGGCCCT CAGCTCACTC CAGGGATGAT GTCAGAAAAT 
GAGGTTCTAA ACATGCAGCT TTCGGATGGA GGACAAGGAG ATGTCCCTGT TGATGAAAAC 
AAACTCCACG GTAAACCTGA TAAAACCTTG CGCTTTTCCC TCTGCAGTGA TAATCTGGAA 
GGCATATCTG AAGGTCCTTC CAATCGCTCC AATTCGGTGT CTTCTCTCGA CCTGGAGGGA 
GAGTCTGTAT CTGAACTTGG GGCAGGACCT TCTGGGAGTA ACGGAGTTGA AGCACTGCAG 
CTGTTAGAGC ACGAGCAAGC TACAACACAA GATAATCTTG ATGATAAACT GAGGAAGTTT 
GAGATTCGTG ACATGATGGG ACTAACAGAT GACAGGGACA TATCCGAGAC TGTGAGTGAG 
ACCTGGAGCA CTGATGTCCT GGGAAGTGAC TTCGACCCGA ACGTTGATGA AGATCGTTTG 
CAAGAGATTG CAGGTGCAGC AGCAGAGAAC GTGTTAGGTA GTTTGCTGTG CCTTCCAGGT 
TCAGGGTCAG TTCTCCTTGA CCCTTGCACT GGCTCTACCA TATCAGAGAC CACAAGTGAA 
GCTTGGAGTG TAGAGGTATT GCCAAGTGAC TCAGAGGCTC CAGACTTAAA GCAGGAAGAG 
CGCCTCCAAG AGCTGGAGAG CTGTTCTGGA CTGGGCAGCA CATCTGATGA TACGGATGTC 
AGGGAGGTCA GTTCCCGCCC CAGCACACCA GGCCTCAGTG TTGTGTCCGG TATAAGTGCT 
ACATCGGAGG ATATTCCCAA TAAGATCGAA GACCTGAGAT CTGAGTGCAG CTCTGATTTT 
GGGGGCAAAG ATTCTGTAAC TAGTCCAGAT ATGGATGACA TAGCCCATGG CGCTCATCAG 
CTGACCTCTC CTCCCTCTCA GTCAGAGTCT CTGCTTGCCA TGTTTGATCC ACTGTCCTCA 
CATGAAGGGG CCTCTGCTGT GGTAAGGCCA AAGGTTCACT ATGCCCGACC CTCGCATCCA 
CCTCCGGATC CTCCAATCCT GGAAGGTGCA GTGGGAGGAA ATGAGGCCAG GTTGCCAAAC 
TTTGGCTCTC ACGTTTTAAC TGCAGCTGAA ATGGAGGCGT TCAAGCAGAG ACACTCATAC 
CCTGAGAGAT TGGTTCGCAG CAGAAGCTCT GATATAGTGT CTTCTGTGCG GCGGCCCATG 
AGTGACCCCA GCTGGAACCG ACGTCCAGGG AATGAAGAAC TTCCTCCAGC TGCAGCCACT 
GGTGCTACTT CTTTGGTGGC TGCACCTCAC TCGTCATCTT CGTCCCCGAG TAAGGACTCT 
TCTAGAGGAG AGACTGAGGA ACGCAAGGAC AGTGACGATG AGAGGTCAGA CAGGAGCAGG 
CCTTGGTGGA GGAAGCGCTT TGTTTCAGCT ATGCCTAAAG CTCCTATACC ATTTAGAAAG 
AAAGAAAAAC AAGAAAAAGA CAAAGATGAT CTGGGTCCTG ACAGATTCTC AACACTCACA 
GATGAGCCCA GCCCTAGGCT CAGTGCTCAA GCTCAGGTTG CTGAAGATAT TCTGGACAAG 
TACCGGAATG CCATTAAACG GACCAGCCCC AGTGAAGGAG CAATGGCAAA TGATGAGAGT 
GCAGAGGTCA TGGGTGATGG TGAAAGTGCC CACGACTCTC CTCGTGAGGA AGCACTGCAG 
AACATCTCAG CCGACGACCT CCCGGACTCC GCCAGCCAGG CAGCCCACCC TCAGGACTCT 
GCTTTCTCTT ACAGAGATGT AAAAAAGAAG CTGAGGCTCG CACTTTGCTC TGCAGACTCT 
GTGGCCTTCC CAGTGCTAAC TCATTCAACA AGGAATGGAT TGCCAGACCA CACAGACCCA 
GAAGACAATG AAATTGTATG CTTCTTAAAA GTTCAAATAG CTGAAGCAAT TAATTTACAA 
GATAAGAGCT TAATGGCTCA ACTTCAAGAA ACAATGCGTT GTGTGTGCCG CTTTGACAAT 
AGGACTTGTA GGAAGTTGCT GGCATCCATT GCTGAAGACT ACAGAAAAAG AGCTCCGTAT 
ATTGCTTATC TTACCCGCTG CCGACAAGGA CTGCAGACCA CACAGGCTCA CTTGGAAAGG 
CTCTTGCAAA GAGTTTTGCG GGACAAAGAG GTGGCCAATC GGTACTTCAC CACTGTCTGT 
GTGCGGTTAC TGCTTGAGAG CAAAGAAAAG AAAATCAGGG AGTTCATTCA AGACTTTCAG 
AAACTTACTG CTGCTGATGA TAAAACGGCT CAGGTGGAGG ATTTTCTACA GTTCCTGTAT 
GGTGTGATGG CTCAGGATGT CATATGGCAG AATGCCAGTG AGGAGCAGCT GCAGGATGCC 
CAGCTGGCCA TCGAGCGGAG TGTGATGAAT CGGATCTTCA AGCTTGCTTT CTACCCTAAC 
CAGGATGGGG ACATACTTCG TGACCAGGTT CTGCATGAAC ATATCCAGAG ATTATCTAAA 
GTAGTGACTG CAAACCACAG AGCTCTGCAG ATCCCTGAGG TTTATCTCCG TGAGGCACCA 
TGGCCATCTG CACAGTCAGA AATCAGGACA ATAAGTGCTT ACAAAACGCC CCGCGACAAA 
GTGCAGTGCA TCCTGAGGAT GTGCTCCACC ATCATGAACC TCTTGAGCCT GGCCAACGAG 
GACTCTGTCC CCGGGGCAGA TGACTTTGTT CCTGTGTTGG TGTTTGTGTT AATAAAGGCA 
AACCCACCCT GCCTGCTGTC CACTGTGCAG TACATCAGCA GCTTCTATGC CAGCTGTCTG 
TCTGGAGAGG AGTCCTACTG GTGGATGCAG TTCACAGCAG CCGTGGAGTT TATTAAAACC 
ATCGATGACA GAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAPVD1 ( XM_017319196.1 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_017174685.1 Mus musculus GTPase activating protein and VPS9 domains 1 (Gapvd1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu69988
Clone ID Related Accession (Same CDS sequence)	XM_017319195.2 , XM_017319196.2 , XM_011239145.1 , XM_017319195.1 , XM_017319196.1
Accession Version	XM_017319196.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4458bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	GTPase-activating protein and VPS9 domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGC TAGATATTCA CACATTGGCC CACCACCTCA AGCAGGAACG CTTATATGTG 
AGCTCTGAGA AACAGCTCAT TCAGAGGCTA AATGCCGATG TACTGAAGAC AGCTGAAAAG 
CTGTATCGAA CAGCGTGGAT TGCAAAGCAA CAGAGGATTA ATTTGGATCG TCTTATTATA 
ACCAGTGCTG AAGCTTCCCC TGCTGAGTGT TGCCAGCATG CCAAGATTTT GGAAGATACA 
CAGTTTGTTG ATGGATATAA GCAACTGGGA TTTCAGGAAA CAGCTTATGG AGAATTCCTG 
AGTCGGTTAA GAGAAAATCC ACGACTTATT GCCTCCTCTT TGGTTGCTGG AGAAAAACTC 
AATCAGGAGA ACACACAAAG TGTTATCTAC ACTGTTTTTA CCTCCTTGTA TGGCAACTGT 
ATTATGCAAG AAGATGAAAG CTACCTCCTT CAGGTTTTAC GATACTTGAT TGAATTTGAA 
CTTAAAGAAA GTGACAACCC CAGGCGCCTT TTGAGGAGAG GAACTTGTGC CTTCAGCATC 
TTATTTAAAC TTTTTTCTGA AGGACTGTTT TCTGCCAAAC TTTTCCTTAC GGCTACTTTG 
CATGAGCCAA TCATGCAGCT CCTCGTAGAG GATGAAGACC ACCTGGAAAC AGATCCAAAC 
AAGCTGATAG AGAGATTCTC TCCAGCTCAG CAAGAAAAAC TCTTTGGAGA GAAAGGCTCA 
GATAGATTTA GGCAAAAGGT TCAAGAAATG GTAGATTCCA ATGAGGCTAA ACTAGTGGCT 
TTGGTGAACA AATTTATTGG TTATCTTAAA CAGAATACAT ACTGCTTTCC ACATAGTTTG 
AGGTGGATTG TGTCACAGAT GTACAAAACT CTTTCTTGTG TAGACAGATT GGAGGTTGGG 
GAAGTCAGAG CAATGTGTAC CGATCTTCTG CTGGCTTGCT TCATTTGTCC TGCAGTTGTC 
AATCCAGAGC AGTATGGGAT AATATCTGAT GCTCCTATTA ATGAGGTGGC AAGATTTAAC 
CTGATGCAGG TTGGCCGCCT TTTGCAGCAG CTAGCAATGA CTGGCACTGA AGAAGGAGAT 
CCCCGGACCA AAAATAGCCT TGGAAAATTT GACAAAGGCT GTGTCGCTGC TTTCCTTGAT 
GTTGTAATTG GGGGCCGTGC AGTGGAGACT CCGCCAATGT CCTCTGTTAA TCTTCTGGAA 
GGCTTGAGCA GAACTGTGGT GTATATAAGT TACAGTCAGC TTATTACTCT GGTAAATTTT 
ATGAAGAGTG TGATGTCTGG AGATCAACTG AAAGAAGATA GAATGGCTCT TGACAATTTG 
TTGGCAAACC TGCCCCAGGC TAAGCCAGGG AAGAGCAGCA GTCTGGACAT GACTCCCTAT 
AGCACTCCTC AGATGTCTCC AGCAACCACT CCAGCCAACA AGAAGAATCG ATTACCTATA 
GGACAACAGT TAGCAGCCAT CACTGCCTGG GATTCGTCAG CCACCAATCT TACTGCTCAT 
ATTCCTCTAG TAACCCCATT TGCAACTCGG AGCAGAAGCC GAAGTAATAT GTTAATGGAC 
CTACATATGG ATCATGAAGG ATCATCTCAA GAAACCATCC AGGAGGTACA GCCAGAAGAG 
GTGCTGGTCA TTTCCTTAGG GACAGGCCCT CAGCTCACTC CAGGGATGAT GTCAGAAAAT 
GAGGTTCTAA ACATGCAGCT TTCGGATGGA GGACAAGGAG ATGTCCCTGT TGATGAAAAC 
AAACTCCACG GTAAACCTGA TAAAACCTTG CGCTTTTCCC TCTGCAGTGA TAATCTGGAA 
GGCATATCTG AAGGTCCTTC CAATCGCTCC AATTCGGTGT CTTCTCTCGA CCTGGAGGGA 
GAGTCTGTAT CTGAACTTGG GGCAGGACCT TCTGGGAGTA ACGGAGTTGA AGCACTGCAG 
CTGTTAGAGC ACGAGCAAGC TACAACACAA GATAATCTTG ATGATAAACT GAGGAAGTTT 
GAGATTCGTG ACATGATGGG ACTAACAGAT GACAGGGACA TATCCGAGAC TGTGAGTGAG 
ACCTGGAGCA CTGATGTCCT GGGAAGTGAC TTCGACCCGA ACGTTGATGA AGATCGTTTG 
CAAGAGATTG CAGGTGCAGC AGCAGAGAAC GTGTTAGGTA GTTTGCTGTG CCTTCCAGGT 
TCAGGGTCAG TTCTCCTTGA CCCTTGCACT GGCTCTACCA TATCAGAGAC CACAAGTGAA 
GCTTGGAGTG TAGAGGTATT GCCAAGTGAC TCAGAGGCTC CAGACTTAAA GCAGGAAGAG 
CGCCTCCAAG AGCTGGAGAG CTGTTCTGGA CTGGGCAGCA CATCTGATGA TACGGATGTC 
AGGGAGGTCA GTTCCCGCCC CAGCACACCA GGCCTCAGTG TTGTGTCCGG TATAAGTGCT 
ACATCGGAGG ATATTCCCAA TAAGATCGAA GACCTGAGAT CTGAGTGCAG CTCTGATTTT 
GGGGGCAAAG ATTCTGTAAC TAGTCCAGAT ATGGATGACA TAGCCCATGG CGCTCATCAG 
CTGACCTCTC CTCCCTCTCA GTCAGAGTCT CTGCTTGCCA TGTTTGATCC ACTGTCCTCA 
CATGAAGGGG CCTCTGCTGT GGTAAGGCCA AAGGTTCACT ATGCCCGACC CTCGCATCCA 
CCTCCGGATC CTCCAATCCT GGAAGGTGCA GTGGGAGGAA ATGAGGCCAG GTTGCCAAAC 
TTTGGCTCTC ACGTTTTAAC TGCAGCTGAA ATGGAGGCGT TCAAGCAGAG ACACTCATAC 
CCTGAGAGAT TGGTTCGCAG CAGAAGCTCT GATATAGTGT CTTCTGTGCG GCGGCCCATG 
AGTGACCCCA GCTGGAACCG ACGTCCAGGG AATGAAGAAC TTCCTCCAGC TGCAGCCACT 
GGTGCTACTT CTTTGGTGGC TGCACCTCAC TCGTCATCTT CGTCCCCGAG TAAGGACTCT 
TCTAGAGGAG AGACTGAGGA ACGCAAGGAC AGTGACGATG AGAGGTCAGA CAGGAGCAGG 
CCTTGGTGGA GGAAGCGCTT TGTTTCAGCT ATGCCTAAAG CTCCTATACC ATTTAGAAAG 
AAAGAAAAAC AAGAAAAAGA CAAAGATGAT CTGGGTCCTG ACAGATTCTC AACACTCACA 
GATGAGCCCA GCCCTAGGCT CAGTGCTCAA GCTCAGGTTG CTGAAGATAT TCTGGACAAG 
TACCGGAATG CCATTAAACG GACCAGCCCC AGTGAAGGAG CAATGGCAAA TGATGAGAGT 
GCAGAGGTCA TGGGTGATGG TGAAAGTGCC CACGACTCTC CTCGTGAGGA AGCACTGCAG 
AACATCTCAG CCGACGACCT CCCGGACTCC GCCAGCCAGG CAGCCCACCC TCAGGACTCT 
GCTTTCTCTT ACAGAGATGT AAAAAAGAAG CTGAGGCTCG CACTTTGCTC TGCAGACTCT 
GTGGCCTTCC CAGTGCTAAC TCATTCAACA AGGAATGGAT TGCCAGACCA CACAGACCCA 
GAAGACAATG AAATTGTATG CTTCTTAAAA GTTCAAATAG CTGAAGCAAT TAATTTACAA 
GATAAGAGCT TAATGGCTCA ACTTCAAGAA ACAATGCGTT GTGTGTGCCG CTTTGACAAT 
AGGACTTGTA GGAAGTTGCT GGCATCCATT GCTGAAGACT ACAGAAAAAG AGCTCCGTAT 
ATTGCTTATC TTACCCGCTG CCGACAAGGA CTGCAGACCA CACAGGCTCA CTTGGAAAGG 
CTCTTGCAAA GAGTTTTGCG GGACAAAGAG GTGGCCAATC GGTACTTCAC CACTGTCTGT 
GTGCGGTTAC TGCTTGAGAG CAAAGAAAAG AAAATCAGGG AGTTCATTCA AGACTTTCAG 
AAACTTACTG CTGCTGATGA TAAAACGGCT CAGGTGGAGG ATTTTCTACA GTTCCTGTAT 
GGTGTGATGG CTCAGGATGT CATATGGCAG AATGCCAGTG AGGAGCAGCT GCAGGATGCC 
CAGCTGGCCA TCGAGCGGAG TGTGATGAAT CGGATCTTCA AGCTTGCTTT CTACCCTAAC 
CAGGATGGGG ACATACTTCG TGACCAGGTT CTGCATGAAC ATATCCAGAG ATTATCTAAA 
GTAGTGACTG CAAACCACAG AGCTCTGCAG ATCCCTGAGG TTTATCTCCG TGAGGCACCA 
TGGCCATCTG CACAGTCAGA AATCAGGACA ATAAGTGCTT ACAAAACGCC CCGCGACAAA 
GTGCAGTGCA TCCTGAGGAT GTGCTCCACC ATCATGAACC TCTTGAGCCT GGCCAACGAG 
GACTCTGTCC CCGGGGCAGA TGACTTTGTT CCTGTGTTGG TGTTTGTGTT AATAAAGGCA 
AACCCACCCT GCCTGCTGTC CACTGTGCAG TACATCAGCA GCTTCTATGC CAGCTGTCTG 
TCTGGAGAGG AGTCCTACTG GTGGATGCAG TTCACAGCAG CCGTGGAGTT TATTAAAACC 
ATCGATGACA GAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017174685.1
CDS	178..4635
Translation	MVKLDIHTLAHHLKQERLYVSSEKQLIQRLNADVLKTAEKLYRTAWIAKQQRINLDRLIITSAEASPAECCQHAKILEDTQFVDGYKQLGFQETAYGEFLSRLRENPRLIASSLVAGEKLNQENTQSVIYTVFTSLYGNCIMQEDESYLLQVLRYLIEFELKESDNPRRLLRRGTCAFSILFKLFSEGLFSAKLFLTATLHEPIMQLLVEDEDHLETDPNKLIERFSPAQQEKLFGEKGSDRFRQKVQEMVDSNEAKLVALVNKFIGYLKQNTYCFPHSLRWIVSQMYKTLSCVDRLEVGEVRAMCTDLLLACFICPAVVNPEQYGIISDAPINEVARFNLMQVGRLLQQLAMTGTEEGDPRTKNSLGKFDKGCVAAFLDVVIGGRAVETPPMSSVNLLEGLSRTVVYISYSQLITLVNFMKSVMSGDQLKEDRMALDNLLANLPQAKPGKSSSLDMTPYSTPQMSPATTPANKKNRLPIGQQLAAITAWDSSATNLTAHIPLVTPFATRSRSRSNMLMDLHMDHEGSSQETIQEVQPEEVLVISLGTGPQLTPGMMSENEVLNMQLSDGGQGDVPVDENKLHGKPDKTLRFSLCSDNLEGISEGPSNRSNSVSSLDLEGESVSELGAGPSGSNGVEALQLLEHEQATTQDNLDDKLRKFEIRDMMGLTDDRDISETVSETWSTDVLGSDFDPNVDEDRLQEIAGAAAENVLGSLLCLPGSGSVLLDPCTGSTISETTSEAWSVEVLPSDSEAPDLKQEERLQELESCSGLGSTSDDTDVREVSSRPSTPGLSVVSGISATSEDIPNKIEDLRSECSSDFGGKDSVTSPDMDDIAHGAHQLTSPPSQSESLLAMFDPLSSHEGASAVVRPKVHYARPSHPPPDPPILEGAVGGNEARLPNFGSHVLTAAEMEAFKQRHSYPERLVRSRSSDIVSSVRRPMSDPSWNRRPGNEELPPAAATGATSLVAAPHSSSSSPSKDSSRGETEERKDSDDERSDRSRPWWRKRFVSAMPKAPIPFRKKEKQEKDKDDLGPDRFSTLTDEPSPRLSAQAQVAEDILDKYRNAIKRTSPSEGAMANDESAEVMGDGESAHDSPREEALQNISADDLPDSASQAAHPQDSAFSYRDVKKKLRLALCSADSVAFPVLTHSTRNGLPDHTDPEDNEIVCFLKVQIAEAINLQDKSLMAQLQETMRCVCRFDNRTCRKLLASIAEDYRKRAPYIAYLTRCRQGLQTTQAHLERLLQRVLRDKEVANRYFTTVCVRLLLESKEKKIREFIQDFQKLTAADDKTAQVEDFLQFLYGVMAQDVIWQNASEEQLQDAQLAIERSVMNRIFKLAFYPNQDGDILRDQVLHEHIQRLSKVVTANHRALQIPEVYLREAPWPSAQSEIRTISAYKTPRDKVQCILRMCSTIMNLLSLANEDSVPGADDFVPVLVFVLIKANPPCLLSTVQYISSFYASCLSGEESYWWMQFTAAVEFIKTIDDRK

Target ORF information:

RefSeq Version	XM_017319196.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus GTPase activating protein and VPS9 domains 1 (Gapvd1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319196.1

ORF Insert Sequence:

ATGGTGAAGC TAGATATTCA CACATTGGCC CACCACCTCA AGCAGGAACG CTTATATGTG 
AGCTCTGAGA AACAGCTCAT TCAGAGGCTA AATGCCGATG TACTGAAGAC AGCTGAAAAG 
CTGTATCGAA CAGCGTGGAT TGCAAAGCAA CAGAGGATTA ATTTGGATCG TCTTATTATA 
ACCAGTGCTG AAGCTTCCCC TGCTGAGTGT TGCCAGCATG CCAAGATTTT GGAAGATACA 
CAGTTTGTTG ATGGATATAA GCAACTGGGA TTTCAGGAAA CAGCTTATGG AGAATTCCTG 
AGTCGGTTAA GAGAAAATCC ACGACTTATT GCCTCCTCTT TGGTTGCTGG AGAAAAACTC 
AATCAGGAGA ACACACAAAG TGTTATCTAC ACTGTTTTTA CCTCCTTGTA TGGCAACTGT 
ATTATGCAAG AAGATGAAAG CTACCTCCTT CAGGTTTTAC GATACTTGAT TGAATTTGAA 
CTTAAAGAAA GTGACAACCC CAGGCGCCTT TTGAGGAGAG GAACTTGTGC CTTCAGCATC 
TTATTTAAAC TTTTTTCTGA AGGACTGTTT TCTGCCAAAC TTTTCCTTAC GGCTACTTTG 
CATGAGCCAA TCATGCAGCT CCTCGTAGAG GATGAAGACC ACCTGGAAAC AGATCCAAAC 
AAGCTGATAG AGAGATTCTC TCCAGCTCAG CAAGAAAAAC TCTTTGGAGA GAAAGGCTCA 
GATAGATTTA GGCAAAAGGT TCAAGAAATG GTAGATTCCA ATGAGGCTAA ACTAGTGGCT 
TTGGTGAACA AATTTATTGG TTATCTTAAA CAGAATACAT ACTGCTTTCC ACATAGTTTG 
AGGTGGATTG TGTCACAGAT GTACAAAACT CTTTCTTGTG TAGACAGATT GGAGGTTGGG 
GAAGTCAGAG CAATGTGTAC CGATCTTCTG CTGGCTTGCT TCATTTGTCC TGCAGTTGTC 
AATCCAGAGC AGTATGGGAT AATATCTGAT GCTCCTATTA ATGAGGTGGC AAGATTTAAC 
CTGATGCAGG TTGGCCGCCT TTTGCAGCAG CTAGCAATGA CTGGCACTGA AGAAGGAGAT 
CCCCGGACCA AAAATAGCCT TGGAAAATTT GACAAAGGCT GTGTCGCTGC TTTCCTTGAT 
GTTGTAATTG GGGGCCGTGC AGTGGAGACT CCGCCAATGT CCTCTGTTAA TCTTCTGGAA 
GGCTTGAGCA GAACTGTGGT GTATATAAGT TACAGTCAGC TTATTACTCT GGTAAATTTT 
ATGAAGAGTG TGATGTCTGG AGATCAACTG AAAGAAGATA GAATGGCTCT TGACAATTTG 
TTGGCAAACC TGCCCCAGGC TAAGCCAGGG AAGAGCAGCA GTCTGGACAT GACTCCCTAT 
AGCACTCCTC AGATGTCTCC AGCAACCACT CCAGCCAACA AGAAGAATCG ATTACCTATA 
GGACAACAGT TAGCAGCCAT CACTGCCTGG GATTCGTCAG CCACCAATCT TACTGCTCAT 
ATTCCTCTAG TAACCCCATT TGCAACTCGG AGCAGAAGCC GAAGTAATAT GTTAATGGAC 
CTACATATGG ATCATGAAGG ATCATCTCAA GAAACCATCC AGGAGGTACA GCCAGAAGAG 
GTGCTGGTCA TTTCCTTAGG GACAGGCCCT CAGCTCACTC CAGGGATGAT GTCAGAAAAT 
GAGGTTCTAA ACATGCAGCT TTCGGATGGA GGACAAGGAG ATGTCCCTGT TGATGAAAAC 
AAACTCCACG GTAAACCTGA TAAAACCTTG CGCTTTTCCC TCTGCAGTGA TAATCTGGAA 
GGCATATCTG AAGGTCCTTC CAATCGCTCC AATTCGGTGT CTTCTCTCGA CCTGGAGGGA 
GAGTCTGTAT CTGAACTTGG GGCAGGACCT TCTGGGAGTA ACGGAGTTGA AGCACTGCAG 
CTGTTAGAGC ACGAGCAAGC TACAACACAA GATAATCTTG ATGATAAACT GAGGAAGTTT 
GAGATTCGTG ACATGATGGG ACTAACAGAT GACAGGGACA TATCCGAGAC TGTGAGTGAG 
ACCTGGAGCA CTGATGTCCT GGGAAGTGAC TTCGACCCGA ACGTTGATGA AGATCGTTTG 
CAAGAGATTG CAGGTGCAGC AGCAGAGAAC GTGTTAGGTA GTTTGCTGTG CCTTCCAGGT 
TCAGGGTCAG TTCTCCTTGA CCCTTGCACT GGCTCTACCA TATCAGAGAC CACAAGTGAA 
GCTTGGAGTG TAGAGGTATT GCCAAGTGAC TCAGAGGCTC CAGACTTAAA GCAGGAAGAG 
CGCCTCCAAG AGCTGGAGAG CTGTTCTGGA CTGGGCAGCA CATCTGATGA TACGGATGTC 
AGGGAGGTCA GTTCCCGCCC CAGCACACCA GGCCTCAGTG TTGTGTCCGG TATAAGTGCT 
ACATCGGAGG ATATTCCCAA TAAGATCGAA GACCTGAGAT CTGAGTGCAG CTCTGATTTT 
GGGGGCAAAG ATTCTGTAAC TAGTCCAGAT ATGGATGACA TAGCCCATGG CGCTCATCAG 
CTGACCTCTC CTCCCTCTCA GTCAGAGTCT CTGCTTGCCA TGTTTGATCC ACTGTCCTCA 
CATGAAGGGG CCTCTGCTGT GGTAAGGCCA AAGGTTCACT ATGCCCGACC CTCGCATCCA 
CCTCCGGATC CTCCAATCCT GGAAGGTGCA GTGGGAGGAA ATGAGGCCAG GTTGCCAAAC 
TTTGGCTCTC ACGTTTTAAC TGCAGCTGAA ATGGAGGCGT TCAAGCAGAG ACACTCATAC 
CCTGAGAGAT TGGTTCGCAG CAGAAGCTCT GATATAGTGT CTTCTGTGCG GCGGCCCATG 
AGTGACCCCA GCTGGAACCG ACGTCCAGGG AATGAAGAAC TTCCTCCAGC TGCAGCCACT 
GGTGCTACTT CTTTGGTGGC TGCACCTCAC TCGTCATCTT CGTCCCCGAG TAAGGACTCT 
TCTAGAGGAG AGACTGAGGA ACGCAAGGAC AGTGACGATG AGAGGTCAGA CAGGAGCAGG 
CCTTGGTGGA GGAAGCGCTT TGTTTCAGCT ATGCCTAAAG CTCCTATACC ATTTAGAAAG 
AAAGAAAAAC AAGAAAAAGA CAAAGATGAT CTGGGTCCTG ACAGATTCTC AACACTCACA 
GATGAGCCCA GCCCTAGGCT CAGTGCTCAA GCTCAGGTTG CTGAAGATAT TCTGGACAAG 
TACCGGAATG CCATTAAACG GACCAGCCCC AGTGAAGGAG CAATGGCAAA TGATGAGAGT 
GCAGAGGTCA TGGGTGATGG TGAAAGTGCC CACGACTCTC CTCGTGAGGA AGCACTGCAG 
AACATCTCAG CCGACGACCT CCCGGACTCC GCCAGCCAGG CAGCCCACCC TCAGGACTCT 
GCTTTCTCTT ACAGAGATGT AAAAAAGAAG CTGAGGCTCG CACTTTGCTC TGCAGACTCT 
GTGGCCTTCC CAGTGCTAAC TCATTCAACA AGGAATGGAT TGCCAGACCA CACAGACCCA 
GAAGACAATG AAATTGTATG CTTCTTAAAA GTTCAAATAG CTGAAGCAAT TAATTTACAA 
GATAAGAGCT TAATGGCTCA ACTTCAAGAA ACAATGCGTT GTGTGTGCCG CTTTGACAAT 
AGGACTTGTA GGAAGTTGCT GGCATCCATT GCTGAAGACT ACAGAAAAAG AGCTCCGTAT 
ATTGCTTATC TTACCCGCTG CCGACAAGGA CTGCAGACCA CACAGGCTCA CTTGGAAAGG 
CTCTTGCAAA GAGTTTTGCG GGACAAAGAG GTGGCCAATC GGTACTTCAC CACTGTCTGT 
GTGCGGTTAC TGCTTGAGAG CAAAGAAAAG AAAATCAGGG AGTTCATTCA AGACTTTCAG 
AAACTTACTG CTGCTGATGA TAAAACGGCT CAGGTGGAGG ATTTTCTACA GTTCCTGTAT 
GGTGTGATGG CTCAGGATGT CATATGGCAG AATGCCAGTG AGGAGCAGCT GCAGGATGCC 
CAGCTGGCCA TCGAGCGGAG TGTGATGAAT CGGATCTTCA AGCTTGCTTT CTACCCTAAC 
CAGGATGGGG ACATACTTCG TGACCAGGTT CTGCATGAAC ATATCCAGAG ATTATCTAAA 
GTAGTGACTG CAAACCACAG AGCTCTGCAG ATCCCTGAGG TTTATCTCCG TGAGGCACCA 
TGGCCATCTG CACAGTCAGA AATCAGGACA ATAAGTGCTT ACAAAACGCC CCGCGACAAA 
GTGCAGTGCA TCCTGAGGAT GTGCTCCACC ATCATGAACC TCTTGAGCCT GGCCAACGAG 
GACTCTGTCC CCGGGGCAGA TGACTTTGTT CCTGTGTTGG TGTTTGTGTT AATAAAGGCA 
AACCCACCCT GCCTGCTGTC CACTGTGCAG TACATCAGCA GCTTCTATGC CAGCTGTCTG 
TCTGGAGAGG AGTCCTACTG GTGGATGCAG TTCACAGCAG CCGTGGAGTT TATTAAAACC 
ATCGATGACA GAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAPVD1 ( XM_011239147.1 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_011237449.1 Mus musculus GTPase activating protein and VPS9 domains 1 (Gapvd1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu69989
Clone ID Related Accession (Same CDS sequence)	XM_030251954.1 , XM_011239147.1
Accession Version	XM_011239147.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	GTPase-activating protein and VPS9 domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGC TAGATATTCA CACATTGGCC CACCACCTCA AGCAGGAACG CTTATATGTG 
AGCTCTGAGA AACAGCTCAT TCAGAGGCTA AATGCCGATG TACTGAAGAC AGCTGAAAAG 
CTGTATCGAA CAGCGTGGAT TGCAAAGCAA CAGAGGATTA ATTTGGATCG TCTTATTATA 
ACCAGTGCTG AAGCTTCCCC TGCTGAGTGT TGCCAGCATG CCAAGATTTT GGAAGATACA 
CAGTTTGTTG ATGGATATAA GCAACTGGGA TTTCAGGAAA CAGCTTATGG AGAATTCCTG 
AGTCGGTTAA GAGAAAATCC ACGACTTATT GCCTCCTCTT TGGTTGCTGG AGAAAAACTC 
AATCAGGAGA ACACACAAAG TGTTATCTAC ACTGTTTTTA CCTCCTTGTA TGGCAACTGT 
ATTATGCAAG AAGATGAAAG CTACCTCCTT CAGGTTTTAC GATACTTGAT TGAATTTGAA 
CTTAAAGAAA GTGACAACCC CAGGCGCCTT TTGAGGAGAG GAACTTGTGC CTTCAGCATC 
TTATTTAAAC TTTTTTCTGA AGGACTGTTT TCTGCCAAAC TTTTCCTTAC GGCTACTTTG 
CATGAGCCAA TCATGCAGCT CCTCGTAGAG GATGAAGACC ACCTGGAAAC AGATCCAAAC 
AAGCTGATAG AGAGATTCTC TCCAGCTCAG CAAGAAAAAC TCTTTGGAGA GAAAGGCTCA 
GATAGATTTA GGCAAAAGGT TCAAGAAATG GTAGATTCCA ATGAGGCTAA ACTAGTGGCT 
TTGGTGAACA AATTTATTGG TTATCTTAAA CAGAATACAT ACTGCTTTCC ACATAGTTTG 
AGGTGGATTG TGTCACAGAT GTACAAAACT CTTTCTTGTG TAGACAGATT GGAGGTTGGG 
GAAGTCAGAG CAATGTGTAC CGATCTTCTG CTGGCTTGCT TCATTTGTCC TGCAGTTGTC 
AATCCAGAGC AGTATGGGAT AATATCTGAT GCTCCTATTA ATGAGGTGGC AAGATTTAAC 
CTGATGCAGG TTGGCCGCCT TTTGCAGCAG CTAGCAATGA CTGGCACTGA AGAAGGAGAT 
CCCCGGACCA AAAATAGCCT TGGAAAATTT GACAAAGGCT GTGTCGCTGC TTTCCTTGAT 
GTTGTAATTG GGGGCCGTGC AGTGGAGACT CCGCCAATGT CCTCTGTTAA TCTTCTGGAA 
GGCTTGAGCA GAACTGTGGT GTATATAAGT TACAGTCAGC TTATTACTCT GGTAAATTTT 
ATGAAGAGTG TGATGTCTGG AGATCAACTG AAAGAAGATA GAATGGCTCT TGACAATTTG 
TTGGCAAACC TGCCCCAGGC TAAGCCAGGG AAGAGCAGCA GTCTGGACAT GACTCCCTAT 
AGCACTCCTC AGATGTCTCC AGCAACCACT CCAGCCAACA AGAAGAATCG ATTACCTATA 
GGACAACAGT TAGCAGCCAT CACTGCCTGG GATTCGTCAG CCACCAATCT TACTGCTCAT 
ATTCCTCTAG TAACCCCATT TGCAACTCGG AGCAGAAGCC GAAGTAATAT GTTAATGGAC 
CTACATATGG ATCATGAAGG ATCATCTCAA GAAACCATCC AGGAGGTACA GCCAGAAGAG 
GTGCTGGTCA TTTCCTTAGG GACAGGCCCT CAGCTCACTC CAGGGATGAT GTCAGAAAAT 
GAGGTTCTAA ACATGCAGCT TTCGGATGGA GGACAAGGAG ATGTCCCTGT TGATGAAAAC 
AAACTCCACG GTCCTTCCAA TCGCTCCAAT TCGGTGTCTT CTCTCGACCT GGAGGGAGAG 
TCTGTATCTG AACTTGGGGC AGGACCTTCT GGGAGTAACG GAGTTGAAGC ACTGCAGCTG 
TTAGAGCACG AGCAAGCTAC AACACAAGAT AATCTTGATG ATAAACTGAG GAAGTTTGAG 
ATTCGTGACA TGATGGGACT AACAGATGAC AGGGACATAT CCGAGACTGT GAGTGAGACC 
TGGAGCACTG ATGTCCTGGG AAGTGACTTC GACCCGAACG TTGATGAAGA TCGTTTGCAA 
GAGATTGCAG GTGCAGCAGC AGAGAACGTG TTAGGTAGTT TGCTGTGCCT TCCAGGTTCA 
GGGTCAGTTC TCCTTGACCC TTGCACTGGC TCTACCATAT CAGAGACCAC AAGTGAAGCT 
TGGAGTGTAG AGGTATTGCC AAGTGACTCA GAGGCTCCAG ACTTAAAGCA GGAAGAGCGC 
CTCCAAGAGC TGGAGAGCTG TTCTGGACTG GGCAGCACAT CTGATGATAC GGATGTCAGG 
GAGGTCAGTT CCCGCCCCAG CACACCAGGC CTCAGTGTTG TGTCCGGTAT AAGTGCTACA 
TCGGAGGATA TTCCCAATAA GATCGAAGAC CTGAGATCTG AGTGCAGCTC TGATTTTGGG 
GGCAAAGATT CTGTAACTAG TCCAGATATG GATGACATAG CCCATGGCGC TCATCAGCTG 
ACCTCTCCTC CCTCTCAGTC AGAGTCTCTG CTTGCCATGT TTGATCCACT GTCCTCACAT 
GAAGGGGCCT CTGCTGTGGT AAGGCCAAAG GTTCACTATG CCCGACCCTC GCATCCACCT 
CCGGATCCTC CAATCCTGGA AGGTGCAGTG GGAGGAAATG AGGCCAGGTT GCCAAACTTT 
GGCTCTCACG TTTTAACTGC AGCTGAAATG GAGGCGTTCA AGCAGAGACA CTCATACCCT 
GAGAGATTGG TTCGCAGCAG AAGCTCTGAT ATAGTGTCTT CTGTGCGGCG GCCCATGAGT 
GACCCCAGCT GGAACCGACG TCCAGGGAAT GAAGAACTTC CTCCAGCTGC AGCCACTGGT 
GCTACTTCTT TGGTGGCTGC ACCTCACTCG TCATCTTCGT CCCCGAGTAA GGACTCTTCT 
AGAGGAGAGA CTGAGGAACG CAAGGACAGT GACGATGAGA GGTCAGACAG GAGCAGGCCT 
TGGTGGAGGA AGCGCTTTGT TTCAGCTATG CCTAAAGCTC CTATACCATT TAGAAAGAAA 
GAAAAACAAG AAAAAGACAA AGATGATCTG GGTCCTGACA GATTCTCAAC ACTCACAGAT 
GAGCCCAGCC CTAGGCTCAG TGCTCAAGCT CAGGTTGCTG AAGATATTCT GGACAAGTAC 
CGGAATGCCA TTAAACGGAC CAGCCCCAGT GAAGGAGCAA TGGCAAATGA TGAGAGTGCA 
GAGGTCATGG GTGATGGTGA AAGTGCCCAC GACTCTCCTC GTGAGGAAGC ACTGCAGAAC 
ATCTCAGCCG ACGACCTCCC GGACTCCGCC AGCCAGGCAG CCCACCCTCA GGACTCTGCT 
TTCTCTTACA GAGATGTAAA AAAGAAGCTG AGGCTCGCAC TTTGCTCTGC AGACTCTGTG 
GCCTTCCCAG TGCTAACTCA TTCAACAAGG AATGGATTGC CAGACCACAC AGACCCAGAA 
GACAATGAAA TTGTATGCTT CTTAAAAGTT CAAATAGCTG AAGCAATTAA TTTACAAGAT 
AAGAGCTTAA TGGCTCAACT TCAAGAAACA ATGCGTTGTG TGTGCCGCTT TGACAATAGG 
ACTTGTAGGA AGTTGCTGGC ATCCATTGCT GAAGACTACA GAAAAAGAGC TCCGTATATT 
GCTTATCTTA CCCGCTGCCG ACAAGGACTG CAGACCACAC AGGCTCACTT GGAAAGGCTC 
TTGCAAAGAG TTTTGCGGGA CAAAGAGGTG GCCAATCGGT ACTTCACCAC TGTCTGTGTG 
CGGTTACTGC TTGAGAGCAA AGAAAAGAAA ATCAGGGAGT TCATTCAAGA CTTTCAGAAA 
CTTACTGCTG CTGATGATAA AACGGCTCAG GTGGAGGATT TTCTACAGTT CCTGTATGGT 
GTGATGGCTC AGGATGTCAT ATGGCAGAAT GCCAGTGAGG AGCAGCTGCA GGATGCCCAG 
CTGGCCATCG AGCGGAGTGT GATGAATCGG ATCTTCAAGC TTGCTTTCTA CCCTAACCAG 
GATGGGGACA TACTTCGTGA CCAGGTTCTG CATGAACATA TCCAGAGATT ATCTAAAGTA 
GTGACTGCAA ACCACAGAGC TCTGCAGATC CCTGAGGTTT ATCTCCGTGA GGCACCATGG 
CCATCTGCAC AGTCAGAAAT CAGGACAATA AGTGCTTACA AAACGCCCCG CGACAAAGTG 
CAGTGCATCC TGAGGATGTG CTCCACCATC ATGAACCTCT TGAGCCTGGC CAACGAGGAC 
TCTGTCCCCG GGGCAGATGA CTTTGTTCCT GTGTTGGTGT TTGTGTTAAT AAAGGCAAAC 
CCACCCTGCC TGCTGTCCAC TGTGCAGTAC ATCAGCAGCT TCTATGCCAG CTGTCTGTCT 
GGAGAGGAGT CCTACTGGTG GATGCAGTTC ACAGCAGCCG TGGAGTTTAT TAAAACCATC 
GATGACAGAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011237449.1
CDS	593..4987
Translation	MVKLDIHTLAHHLKQERLYVSSEKQLIQRLNADVLKTAEKLYRTAWIAKQQRINLDRLIITSAEASPAECCQHAKILEDTQFVDGYKQLGFQETAYGEFLSRLRENPRLIASSLVAGEKLNQENTQSVIYTVFTSLYGNCIMQEDESYLLQVLRYLIEFELKESDNPRRLLRRGTCAFSILFKLFSEGLFSAKLFLTATLHEPIMQLLVEDEDHLETDPNKLIERFSPAQQEKLFGEKGSDRFRQKVQEMVDSNEAKLVALVNKFIGYLKQNTYCFPHSLRWIVSQMYKTLSCVDRLEVGEVRAMCTDLLLACFICPAVVNPEQYGIISDAPINEVARFNLMQVGRLLQQLAMTGTEEGDPRTKNSLGKFDKGCVAAFLDVVIGGRAVETPPMSSVNLLEGLSRTVVYISYSQLITLVNFMKSVMSGDQLKEDRMALDNLLANLPQAKPGKSSSLDMTPYSTPQMSPATTPANKKNRLPIGQQLAAITAWDSSATNLTAHIPLVTPFATRSRSRSNMLMDLHMDHEGSSQETIQEVQPEEVLVISLGTGPQLTPGMMSENEVLNMQLSDGGQGDVPVDENKLHGPSNRSNSVSSLDLEGESVSELGAGPSGSNGVEALQLLEHEQATTQDNLDDKLRKFEIRDMMGLTDDRDISETVSETWSTDVLGSDFDPNVDEDRLQEIAGAAAENVLGSLLCLPGSGSVLLDPCTGSTISETTSEAWSVEVLPSDSEAPDLKQEERLQELESCSGLGSTSDDTDVREVSSRPSTPGLSVVSGISATSEDIPNKIEDLRSECSSDFGGKDSVTSPDMDDIAHGAHQLTSPPSQSESLLAMFDPLSSHEGASAVVRPKVHYARPSHPPPDPPILEGAVGGNEARLPNFGSHVLTAAEMEAFKQRHSYPERLVRSRSSDIVSSVRRPMSDPSWNRRPGNEELPPAAATGATSLVAAPHSSSSSPSKDSSRGETEERKDSDDERSDRSRPWWRKRFVSAMPKAPIPFRKKEKQEKDKDDLGPDRFSTLTDEPSPRLSAQAQVAEDILDKYRNAIKRTSPSEGAMANDESAEVMGDGESAHDSPREEALQNISADDLPDSASQAAHPQDSAFSYRDVKKKLRLALCSADSVAFPVLTHSTRNGLPDHTDPEDNEIVCFLKVQIAEAINLQDKSLMAQLQETMRCVCRFDNRTCRKLLASIAEDYRKRAPYIAYLTRCRQGLQTTQAHLERLLQRVLRDKEVANRYFTTVCVRLLLESKEKKIREFIQDFQKLTAADDKTAQVEDFLQFLYGVMAQDVIWQNASEEQLQDAQLAIERSVMNRIFKLAFYPNQDGDILRDQVLHEHIQRLSKVVTANHRALQIPEVYLREAPWPSAQSEIRTISAYKTPRDKVQCILRMCSTIMNLLSLANEDSVPGADDFVPVLVFVLIKANPPCLLSTVQYISSFYASCLSGEESYWWMQFTAAVEFIKTIDDRK

Target ORF information:

RefSeq Version	XM_011239147.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus GTPase activating protein and VPS9 domains 1 (Gapvd1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011239147.1

ORF Insert Sequence:

ATGGTGAAGC TAGATATTCA CACATTGGCC CACCACCTCA AGCAGGAACG CTTATATGTG 
AGCTCTGAGA AACAGCTCAT TCAGAGGCTA AATGCCGATG TACTGAAGAC AGCTGAAAAG 
CTGTATCGAA CAGCGTGGAT TGCAAAGCAA CAGAGGATTA ATTTGGATCG TCTTATTATA 
ACCAGTGCTG AAGCTTCCCC TGCTGAGTGT TGCCAGCATG CCAAGATTTT GGAAGATACA 
CAGTTTGTTG ATGGATATAA GCAACTGGGA TTTCAGGAAA CAGCTTATGG AGAATTCCTG 
AGTCGGTTAA GAGAAAATCC ACGACTTATT GCCTCCTCTT TGGTTGCTGG AGAAAAACTC 
AATCAGGAGA ACACACAAAG TGTTATCTAC ACTGTTTTTA CCTCCTTGTA TGGCAACTGT 
ATTATGCAAG AAGATGAAAG CTACCTCCTT CAGGTTTTAC GATACTTGAT TGAATTTGAA 
CTTAAAGAAA GTGACAACCC CAGGCGCCTT TTGAGGAGAG GAACTTGTGC CTTCAGCATC 
TTATTTAAAC TTTTTTCTGA AGGACTGTTT TCTGCCAAAC TTTTCCTTAC GGCTACTTTG 
CATGAGCCAA TCATGCAGCT CCTCGTAGAG GATGAAGACC ACCTGGAAAC AGATCCAAAC 
AAGCTGATAG AGAGATTCTC TCCAGCTCAG CAAGAAAAAC TCTTTGGAGA GAAAGGCTCA 
GATAGATTTA GGCAAAAGGT TCAAGAAATG GTAGATTCCA ATGAGGCTAA ACTAGTGGCT 
TTGGTGAACA AATTTATTGG TTATCTTAAA CAGAATACAT ACTGCTTTCC ACATAGTTTG 
AGGTGGATTG TGTCACAGAT GTACAAAACT CTTTCTTGTG TAGACAGATT GGAGGTTGGG 
GAAGTCAGAG CAATGTGTAC CGATCTTCTG CTGGCTTGCT TCATTTGTCC TGCAGTTGTC 
AATCCAGAGC AGTATGGGAT AATATCTGAT GCTCCTATTA ATGAGGTGGC AAGATTTAAC 
CTGATGCAGG TTGGCCGCCT TTTGCAGCAG CTAGCAATGA CTGGCACTGA AGAAGGAGAT 
CCCCGGACCA AAAATAGCCT TGGAAAATTT GACAAAGGCT GTGTCGCTGC TTTCCTTGAT 
GTTGTAATTG GGGGCCGTGC AGTGGAGACT CCGCCAATGT CCTCTGTTAA TCTTCTGGAA 
GGCTTGAGCA GAACTGTGGT GTATATAAGT TACAGTCAGC TTATTACTCT GGTAAATTTT 
ATGAAGAGTG TGATGTCTGG AGATCAACTG AAAGAAGATA GAATGGCTCT TGACAATTTG 
TTGGCAAACC TGCCCCAGGC TAAGCCAGGG AAGAGCAGCA GTCTGGACAT GACTCCCTAT 
AGCACTCCTC AGATGTCTCC AGCAACCACT CCAGCCAACA AGAAGAATCG ATTACCTATA 
GGACAACAGT TAGCAGCCAT CACTGCCTGG GATTCGTCAG CCACCAATCT TACTGCTCAT 
ATTCCTCTAG TAACCCCATT TGCAACTCGG AGCAGAAGCC GAAGTAATAT GTTAATGGAC 
CTACATATGG ATCATGAAGG ATCATCTCAA GAAACCATCC AGGAGGTACA GCCAGAAGAG 
GTGCTGGTCA TTTCCTTAGG GACAGGCCCT CAGCTCACTC CAGGGATGAT GTCAGAAAAT 
GAGGTTCTAA ACATGCAGCT TTCGGATGGA GGACAAGGAG ATGTCCCTGT TGATGAAAAC 
AAACTCCACG GTCCTTCCAA TCGCTCCAAT TCGGTGTCTT CTCTCGACCT GGAGGGAGAG 
TCTGTATCTG AACTTGGGGC AGGACCTTCT GGGAGTAACG GAGTTGAAGC ACTGCAGCTG 
TTAGAGCACG AGCAAGCTAC AACACAAGAT AATCTTGATG ATAAACTGAG GAAGTTTGAG 
ATTCGTGACA TGATGGGACT AACAGATGAC AGGGACATAT CCGAGACTGT GAGTGAGACC 
TGGAGCACTG ATGTCCTGGG AAGTGACTTC GACCCGAACG TTGATGAAGA TCGTTTGCAA 
GAGATTGCAG GTGCAGCAGC AGAGAACGTG TTAGGTAGTT TGCTGTGCCT TCCAGGTTCA 
GGGTCAGTTC TCCTTGACCC TTGCACTGGC TCTACCATAT CAGAGACCAC AAGTGAAGCT 
TGGAGTGTAG AGGTATTGCC AAGTGACTCA GAGGCTCCAG ACTTAAAGCA GGAAGAGCGC 
CTCCAAGAGC TGGAGAGCTG TTCTGGACTG GGCAGCACAT CTGATGATAC GGATGTCAGG 
GAGGTCAGTT CCCGCCCCAG CACACCAGGC CTCAGTGTTG TGTCCGGTAT AAGTGCTACA 
TCGGAGGATA TTCCCAATAA GATCGAAGAC CTGAGATCTG AGTGCAGCTC TGATTTTGGG 
GGCAAAGATT CTGTAACTAG TCCAGATATG GATGACATAG CCCATGGCGC TCATCAGCTG 
ACCTCTCCTC CCTCTCAGTC AGAGTCTCTG CTTGCCATGT TTGATCCACT GTCCTCACAT 
GAAGGGGCCT CTGCTGTGGT AAGGCCAAAG GTTCACTATG CCCGACCCTC GCATCCACCT 
CCGGATCCTC CAATCCTGGA AGGTGCAGTG GGAGGAAATG AGGCCAGGTT GCCAAACTTT 
GGCTCTCACG TTTTAACTGC AGCTGAAATG GAGGCGTTCA AGCAGAGACA CTCATACCCT 
GAGAGATTGG TTCGCAGCAG AAGCTCTGAT ATAGTGTCTT CTGTGCGGCG GCCCATGAGT 
GACCCCAGCT GGAACCGACG TCCAGGGAAT GAAGAACTTC CTCCAGCTGC AGCCACTGGT 
GCTACTTCTT TGGTGGCTGC ACCTCACTCG TCATCTTCGT CCCCGAGTAA GGACTCTTCT 
AGAGGAGAGA CTGAGGAACG CAAGGACAGT GACGATGAGA GGTCAGACAG GAGCAGGCCT 
TGGTGGAGGA AGCGCTTTGT TTCAGCTATG CCTAAAGCTC CTATACCATT TAGAAAGAAA 
GAAAAACAAG AAAAAGACAA AGATGATCTG GGTCCTGACA GATTCTCAAC ACTCACAGAT 
GAGCCCAGCC CTAGGCTCAG TGCTCAAGCT CAGGTTGCTG AAGATATTCT GGACAAGTAC 
CGGAATGCCA TTAAACGGAC CAGCCCCAGT GAAGGAGCAA TGGCAAATGA TGAGAGTGCA 
GAGGTCATGG GTGATGGTGA AAGTGCCCAC GACTCTCCTC GTGAGGAAGC ACTGCAGAAC 
ATCTCAGCCG ACGACCTCCC GGACTCCGCC AGCCAGGCAG CCCACCCTCA GGACTCTGCT 
TTCTCTTACA GAGATGTAAA AAAGAAGCTG AGGCTCGCAC TTTGCTCTGC AGACTCTGTG 
GCCTTCCCAG TGCTAACTCA TTCAACAAGG AATGGATTGC CAGACCACAC AGACCCAGAA 
GACAATGAAA TTGTATGCTT CTTAAAAGTT CAAATAGCTG AAGCAATTAA TTTACAAGAT 
AAGAGCTTAA TGGCTCAACT TCAAGAAACA ATGCGTTGTG TGTGCCGCTT TGACAATAGG 
ACTTGTAGGA AGTTGCTGGC ATCCATTGCT GAAGACTACA GAAAAAGAGC TCCGTATATT 
GCTTATCTTA CCCGCTGCCG ACAAGGACTG CAGACCACAC AGGCTCACTT GGAAAGGCTC 
TTGCAAAGAG TTTTGCGGGA CAAAGAGGTG GCCAATCGGT ACTTCACCAC TGTCTGTGTG 
CGGTTACTGC TTGAGAGCAA AGAAAAGAAA ATCAGGGAGT TCATTCAAGA CTTTCAGAAA 
CTTACTGCTG CTGATGATAA AACGGCTCAG GTGGAGGATT TTCTACAGTT CCTGTATGGT 
GTGATGGCTC AGGATGTCAT ATGGCAGAAT GCCAGTGAGG AGCAGCTGCA GGATGCCCAG 
CTGGCCATCG AGCGGAGTGT GATGAATCGG ATCTTCAAGC TTGCTTTCTA CCCTAACCAG 
GATGGGGACA TACTTCGTGA CCAGGTTCTG CATGAACATA TCCAGAGATT ATCTAAAGTA 
GTGACTGCAA ACCACAGAGC TCTGCAGATC CCTGAGGTTT ATCTCCGTGA GGCACCATGG 
CCATCTGCAC AGTCAGAAAT CAGGACAATA AGTGCTTACA AAACGCCCCG CGACAAAGTG 
CAGTGCATCC TGAGGATGTG CTCCACCATC ATGAACCTCT TGAGCCTGGC CAACGAGGAC 
TCTGTCCCCG GGGCAGATGA CTTTGTTCCT GTGTTGGTGT TTGTGTTAAT AAAGGCAAAC 
CCACCCTGCC TGCTGTCCAC TGTGCAGTAC ATCAGCAGCT TCTATGCCAG CTGTCTGTCT 
GGAGAGGAGT CCTACTGGTG GATGCAGTTC ACAGCAGCCG TGGAGTTTAT TAAAACCATC 
GATGACAGAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAPVD1 ( XM_011239148.2 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_011237450.1 Mus musculus GTPase activating protein and VPS9 domains 1 (Gapvd1), transcript variant X5, mRNA.

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CloneID	OMu69990
Clone ID Related Accession (Same CDS sequence)	NM_001356441.1 , XM_011239148.2
Accession Version	XM_011239148.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4377bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	GTPase-activating protein and VPS9 domain-containing protein 1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011239148.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGC TAGATATTCA CACATTGGCC CACCACCTCA AGCAGGAACG CTTATATGTG 
AGCTCTGAGA AACAGCTCAT TCAGAGGCTA AATGCCGATG TACTGAAGAC AGCTGAAAAG 
CTGTATCGAA CAGCGTGGAT TGCAAAGCAA CAGAGGATTA ATTTGGATCG TCTTATTATA 
ACCAGTGCTG AAGCTTCCCC TGCTGAGTGT TGCCAGCATG CCAAGATTTT GGAAGATACA 
CAGTTTGTTG ATGGATATAA GCAACTGGGA TTTCAGGAAA CAGCTTATGG AGAATTCCTG 
AGTCGGTTAA GAGAAAATCC ACGACTTATT GCCTCCTCTT TGGTTGCTGG AGAAAAACTC 
AATCAGGAGA ACACACAAAG TGTTATCTAC ACTGTTTTTA CCTCCTTGTA TGGCAACTGT 
ATTATGCAAG AAGATGAAAG CTACCTCCTT CAGGTTTTAC GATACTTGAT TGAATTTGAA 
CTTAAAGAAA GTGACAACCC CAGGCGCCTT TTGAGGAGAG GAACTTGTGC CTTCAGCATC 
TTATTTAAAC TTTTTTCTGA AGGACTGTTT TCTGCCAAAC TTTTCCTTAC GGCTACTTTG 
CATGAGCCAA TCATGCAGCT CCTCGTAGAG GATGAAGACC ACCTGGAAAC AGATCCAAAC 
AAGCTGATAG AGAGATTCTC TCCAGCTCAG CAAGAAAAAC TCTTTGGAGA GAAAGGCTCA 
GATAGATTTA GGCAAAAGGT TCAAGAAATG GTAGATTCCA ATGAGGCTAA ACTAGTGGCT 
TTGGTGAACA AATTTATTGG TTATCTTAAA CAGAATACAT ACTGCTTTCC ACATAGTTTG 
AGGTGGATTG TGTCACAGAT GTACAAAACT CTTTCTTGTG TAGACAGATT GGAGGTTGGG 
GAAGTCAGAG CAATGTGTAC CGATCTTCTG CTGGCTTGCT TCATTTGTCC TGCAGTTGTC 
AATCCAGAGC AGTATGGGAT AATATCTGAT GCTCCTATTA ATGAGGTGGC AAGATTTAAC 
CTGATGCAGG TTGGCCGCCT TTTGCAGCAG CTAGCAATGA CTGGCACTGA AGAAGGAGAT 
CCCCGGACCA AAAATAGCCT TGGAAAATTT GACAAAGGCT GTGTCGCTGC TTTCCTTGAT 
GTTGTAATTG GGGGCCGTGC AGTGGAGACT CCGCCAATGT CCTCTGTTAA TCTTCTGGAA 
GGCTTGAGCA GAACTGTGGT GTATATAAGT TACAGTCAGC TTATTACTCT GGTAAATTTT 
ATGAAGAGTG TGATGTCTGG AGATCAACTG AAAGAAGATA GAATGGCTCT TGACAATTTG 
TTGGCAAACC TGCCCCAGGC TAAGCCAGGG AAGAGCAGCA GTCTGGACAT GACTCCCTAT 
AGCACTCCTC AGATGTCTCC AGCAACCACT CCAGCCAACA AGAAGAATCG ATTACCTATA 
GCAACTCGGA GCAGAAGCCG AAGTAATATG TTAATGGACC TACATATGGA TCATGAAGGA 
TCATCTCAAG AAACCATCCA GGAGGTACAG CCAGAAGAGG TGCTGGTCAT TTCCTTAGGG 
ACAGGCCCTC AGCTCACTCC AGGGATGATG TCAGAAAATG AGGTTCTAAA CATGCAGCTT 
TCGGATGGAG GACAAGGAGA TGTCCCTGTT GATGAAAACA AACTCCACGG TAAACCTGAT 
AAAACCTTGC GCTTTTCCCT CTGCAGTGAT AATCTGGAAG GCATATCTGA AGGTCCTTCC 
AATCGCTCCA ATTCGGTGTC TTCTCTCGAC CTGGAGGGAG AGTCTGTATC TGAACTTGGG 
GCAGGACCTT CTGGGAGTAA CGGAGTTGAA GCACTGCAGC TGTTAGAGCA CGAGCAAGCT 
ACAACACAAG ATAATCTTGA TGATAAACTG AGGAAGTTTG AGATTCGTGA CATGATGGGA 
CTAACAGATG ACAGGGACAT ATCCGAGACT GTGAGTGAGA CCTGGAGCAC TGATGTCCTG 
GGAAGTGACT TCGACCCGAA CGTTGATGAA GATCGTTTGC AAGAGATTGC AGGTGCAGCA 
GCAGAGAACG TGTTAGGTAG TTTGCTGTGC CTTCCAGGTT CAGGGTCAGT TCTCCTTGAC 
CCTTGCACTG GCTCTACCAT ATCAGAGACC ACAAGTGAAG CTTGGAGTGT AGAGGTATTG 
CCAAGTGACT CAGAGGCTCC AGACTTAAAG CAGGAAGAGC GCCTCCAAGA GCTGGAGAGC 
TGTTCTGGAC TGGGCAGCAC ATCTGATGAT ACGGATGTCA GGGAGGTCAG TTCCCGCCCC 
AGCACACCAG GCCTCAGTGT TGTGTCCGGT ATAAGTGCTA CATCGGAGGA TATTCCCAAT 
AAGATCGAAG ACCTGAGATC TGAGTGCAGC TCTGATTTTG GGGGCAAAGA TTCTGTAACT 
AGTCCAGATA TGGATGACAT AGCCCATGGC GCTCATCAGC TGACCTCTCC TCCCTCTCAG 
TCAGAGTCTC TGCTTGCCAT GTTTGATCCA CTGTCCTCAC ATGAAGGGGC CTCTGCTGTG 
GTAAGGCCAA AGGTTCACTA TGCCCGACCC TCGCATCCAC CTCCGGATCC TCCAATCCTG 
GAAGGTGCAG TGGGAGGAAA TGAGGCCAGG TTGCCAAACT TTGGCTCTCA CGTTTTAACT 
GCAGCTGAAA TGGAGGCGTT CAAGCAGAGA CACTCATACC CTGAGAGATT GGTTCGCAGC 
AGAAGCTCTG ATATAGTGTC TTCTGTGCGG CGGCCCATGA GTGACCCCAG CTGGAACCGA 
CGTCCAGGGA ATGAAGAACT TCCTCCAGCT GCAGCCACTG GTGCTACTTC TTTGGTGGCT 
GCACCTCACT CGTCATCTTC GTCCCCGAGT AAGGACTCTT CTAGAGGAGA GACTGAGGAA 
CGCAAGGACA GTGACGATGA GAGGTCAGAC AGGAGCAGGC CTTGGTGGAG GAAGCGCTTT 
GTTTCAGCTA TGCCTAAAGC TCCTATACCA TTTAGAAAGA AAGAAAAACA AGAAAAAGAC 
AAAGATGATC TGGGTCCTGA CAGATTCTCA ACACTCACAG ATGAGCCCAG CCCTAGGCTC 
AGTGCTCAAG CTCAGGTTGC TGAAGATATT CTGGACAAGT ACCGGAATGC CATTAAACGG 
ACCAGCCCCA GTGAAGGAGC AATGGCAAAT GATGAGAGTG CAGAGGTCAT GGGTGATGGT 
GAAAGTGCCC ACGACTCTCC TCGTGAGGAA GCACTGCAGA ACATCTCAGC CGACGACCTC 
CCGGACTCCG CCAGCCAGGC AGCCCACCCT CAGGACTCTG CTTTCTCTTA CAGAGATGTA 
AAAAAGAAGC TGAGGCTCGC ACTTTGCTCT GCAGACTCTG TGGCCTTCCC AGTGCTAACT 
CATTCAACAA GGAATGGATT GCCAGACCAC ACAGACCCAG AAGACAATGA AATTGTATGC 
TTCTTAAAAG TTCAAATAGC TGAAGCAATT AATTTACAAG ATAAGAGCTT AATGGCTCAA 
CTTCAAGAAA CAATGCGTTG TGTGTGCCGC TTTGACAATA GGACTTGTAG GAAGTTGCTG 
GCATCCATTG CTGAAGACTA CAGAAAAAGA GCTCCGTATA TTGCTTATCT TACCCGCTGC 
CGACAAGGAC TGCAGACCAC ACAGGCTCAC TTGGAAAGGC TCTTGCAAAG AGTTTTGCGG 
GACAAAGAGG TGGCCAATCG GTACTTCACC ACTGTCTGTG TGCGGTTACT GCTTGAGAGC 
AAAGAAAAGA AAATCAGGGA GTTCATTCAA GACTTTCAGA AACTTACTGC TGCTGATGAT 
AAAACGGCTC AGGTGGAGGA TTTTCTACAG TTCCTGTATG GTGTGATGGC TCAGGATGTC 
ATATGGCAGA ATGCCAGTGA GGAGCAGCTG CAGGATGCCC AGCTGGCCAT CGAGCGGAGT 
GTGATGAATC GGATCTTCAA GCTTGCTTTC TACCCTAACC AGGATGGGGA CATACTTCGT 
GACCAGGTTC TGCATGAACA TATCCAGAGA TTATCTAAAG TAGTGACTGC AAACCACAGA 
GCTCTGCAGA TCCCTGAGGT TTATCTCCGT GAGGCACCAT GGCCATCTGC ACAGTCAGAA 
ATCAGGACAA TAAGTGCTTA CAAAACGCCC CGCGACAAAG TGCAGTGCAT CCTGAGGATG 
TGCTCCACCA TCATGAACCT CTTGAGCCTG GCCAACGAGG ACTCTGTCCC CGGGGCAGAT 
GACTTTGTTC CTGTGTTGGT GTTTGTGTTA ATAAAGGCAA ACCCACCCTG CCTGCTGTCC 
ACTGTGCAGT ACATCAGCAG CTTCTATGCC AGCTGTCTGT CTGGAGAGGA GTCCTACTGG 
TGGATGCAGT TCACAGCAGC CGTGGAGTTT ATTAAAACCA TCGATGACAG AAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011237450.1
CDS	592..4968
Translation	MVKLDIHTLAHHLKQERLYVSSEKQLIQRLNADVLKTAEKLYRTAWIAKQQRINLDRLIITSAEASPAECCQHAKILEDTQFVDGYKQLGFQETAYGEFLSRLRENPRLIASSLVAGEKLNQENTQSVIYTVFTSLYGNCIMQEDESYLLQVLRYLIEFELKESDNPRRLLRRGTCAFSILFKLFSEGLFSAKLFLTATLHEPIMQLLVEDEDHLETDPNKLIERFSPAQQEKLFGEKGSDRFRQKVQEMVDSNEAKLVALVNKFIGYLKQNTYCFPHSLRWIVSQMYKTLSCVDRLEVGEVRAMCTDLLLACFICPAVVNPEQYGIISDAPINEVARFNLMQVGRLLQQLAMTGTEEGDPRTKNSLGKFDKGCVAAFLDVVIGGRAVETPPMSSVNLLEGLSRTVVYISYSQLITLVNFMKSVMSGDQLKEDRMALDNLLANLPQAKPGKSSSLDMTPYSTPQMSPATTPANKKNRLPIATRSRSRSNMLMDLHMDHEGSSQETIQEVQPEEVLVISLGTGPQLTPGMMSENEVLNMQLSDGGQGDVPVDENKLHGKPDKTLRFSLCSDNLEGISEGPSNRSNSVSSLDLEGESVSELGAGPSGSNGVEALQLLEHEQATTQDNLDDKLRKFEIRDMMGLTDDRDISETVSETWSTDVLGSDFDPNVDEDRLQEIAGAAAENVLGSLLCLPGSGSVLLDPCTGSTISETTSEAWSVEVLPSDSEAPDLKQEERLQELESCSGLGSTSDDTDVREVSSRPSTPGLSVVSGISATSEDIPNKIEDLRSECSSDFGGKDSVTSPDMDDIAHGAHQLTSPPSQSESLLAMFDPLSSHEGASAVVRPKVHYARPSHPPPDPPILEGAVGGNEARLPNFGSHVLTAAEMEAFKQRHSYPERLVRSRSSDIVSSVRRPMSDPSWNRRPGNEELPPAAATGATSLVAAPHSSSSSPSKDSSRGETEERKDSDDERSDRSRPWWRKRFVSAMPKAPIPFRKKEKQEKDKDDLGPDRFSTLTDEPSPRLSAQAQVAEDILDKYRNAIKRTSPSEGAMANDESAEVMGDGESAHDSPREEALQNISADDLPDSASQAAHPQDSAFSYRDVKKKLRLALCSADSVAFPVLTHSTRNGLPDHTDPEDNEIVCFLKVQIAEAINLQDKSLMAQLQETMRCVCRFDNRTCRKLLASIAEDYRKRAPYIAYLTRCRQGLQTTQAHLERLLQRVLRDKEVANRYFTTVCVRLLLESKEKKIREFIQDFQKLTAADDKTAQVEDFLQFLYGVMAQDVIWQNASEEQLQDAQLAIERSVMNRIFKLAFYPNQDGDILRDQVLHEHIQRLSKVVTANHRALQIPEVYLREAPWPSAQSEIRTISAYKTPRDKVQCILRMCSTIMNLLSLANEDSVPGADDFVPVLVFVLIKANPPCLLSTVQYISSFYASCLSGEESYWWMQFTAAVEFIKTIDDRK

Target ORF information:

RefSeq Version	XM_011239148.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus GTPase activating protein and VPS9 domains 1 (Gapvd1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011239148.2

ORF Insert Sequence:

ATGGTGAAGC TAGATATTCA CACATTGGCC CACCACCTCA AGCAGGAACG CTTATATGTG 
AGCTCTGAGA AACAGCTCAT TCAGAGGCTA AATGCCGATG TACTGAAGAC AGCTGAAAAG 
CTGTATCGAA CAGCGTGGAT TGCAAAGCAA CAGAGGATTA ATTTGGATCG TCTTATTATA 
ACCAGTGCTG AAGCTTCCCC TGCTGAGTGT TGCCAGCATG CCAAGATTTT GGAAGATACA 
CAGTTTGTTG ATGGATATAA GCAACTGGGA TTTCAGGAAA CAGCTTATGG AGAATTCCTG 
AGTCGGTTAA GAGAAAATCC ACGACTTATT GCCTCCTCTT TGGTTGCTGG AGAAAAACTC 
AATCAGGAGA ACACACAAAG TGTTATCTAC ACTGTTTTTA CCTCCTTGTA TGGCAACTGT 
ATTATGCAAG AAGATGAAAG CTACCTCCTT CAGGTTTTAC GATACTTGAT TGAATTTGAA 
CTTAAAGAAA GTGACAACCC CAGGCGCCTT TTGAGGAGAG GAACTTGTGC CTTCAGCATC 
TTATTTAAAC TTTTTTCTGA AGGACTGTTT TCTGCCAAAC TTTTCCTTAC GGCTACTTTG 
CATGAGCCAA TCATGCAGCT CCTCGTAGAG GATGAAGACC ACCTGGAAAC AGATCCAAAC 
AAGCTGATAG AGAGATTCTC TCCAGCTCAG CAAGAAAAAC TCTTTGGAGA GAAAGGCTCA 
GATAGATTTA GGCAAAAGGT TCAAGAAATG GTAGATTCCA ATGAGGCTAA ACTAGTGGCT 
TTGGTGAACA AATTTATTGG TTATCTTAAA CAGAATACAT ACTGCTTTCC ACATAGTTTG 
AGGTGGATTG TGTCACAGAT GTACAAAACT CTTTCTTGTG TAGACAGATT GGAGGTTGGG 
GAAGTCAGAG CAATGTGTAC CGATCTTCTG CTGGCTTGCT TCATTTGTCC TGCAGTTGTC 
AATCCAGAGC AGTATGGGAT AATATCTGAT GCTCCTATTA ATGAGGTGGC AAGATTTAAC 
CTGATGCAGG TTGGCCGCCT TTTGCAGCAG CTAGCAATGA CTGGCACTGA AGAAGGAGAT 
CCCCGGACCA AAAATAGCCT TGGAAAATTT GACAAAGGCT GTGTCGCTGC TTTCCTTGAT 
GTTGTAATTG GGGGCCGTGC AGTGGAGACT CCGCCAATGT CCTCTGTTAA TCTTCTGGAA 
GGCTTGAGCA GAACTGTGGT GTATATAAGT TACAGTCAGC TTATTACTCT GGTAAATTTT 
ATGAAGAGTG TGATGTCTGG AGATCAACTG AAAGAAGATA GAATGGCTCT TGACAATTTG 
TTGGCAAACC TGCCCCAGGC TAAGCCAGGG AAGAGCAGCA GTCTGGACAT GACTCCCTAT 
AGCACTCCTC AGATGTCTCC AGCAACCACT CCAGCCAACA AGAAGAATCG ATTACCTATA 
GCAACTCGGA GCAGAAGCCG AAGTAATATG TTAATGGACC TACATATGGA TCATGAAGGA 
TCATCTCAAG AAACCATCCA GGAGGTACAG CCAGAAGAGG TGCTGGTCAT TTCCTTAGGG 
ACAGGCCCTC AGCTCACTCC AGGGATGATG TCAGAAAATG AGGTTCTAAA CATGCAGCTT 
TCGGATGGAG GACAAGGAGA TGTCCCTGTT GATGAAAACA AACTCCACGG TAAACCTGAT 
AAAACCTTGC GCTTTTCCCT CTGCAGTGAT AATCTGGAAG GCATATCTGA AGGTCCTTCC 
AATCGCTCCA ATTCGGTGTC TTCTCTCGAC CTGGAGGGAG AGTCTGTATC TGAACTTGGG 
GCAGGACCTT CTGGGAGTAA CGGAGTTGAA GCACTGCAGC TGTTAGAGCA CGAGCAAGCT 
ACAACACAAG ATAATCTTGA TGATAAACTG AGGAAGTTTG AGATTCGTGA CATGATGGGA 
CTAACAGATG ACAGGGACAT ATCCGAGACT GTGAGTGAGA CCTGGAGCAC TGATGTCCTG 
GGAAGTGACT TCGACCCGAA CGTTGATGAA GATCGTTTGC AAGAGATTGC AGGTGCAGCA 
GCAGAGAACG TGTTAGGTAG TTTGCTGTGC CTTCCAGGTT CAGGGTCAGT TCTCCTTGAC 
CCTTGCACTG GCTCTACCAT ATCAGAGACC ACAAGTGAAG CTTGGAGTGT AGAGGTATTG 
CCAAGTGACT CAGAGGCTCC AGACTTAAAG CAGGAAGAGC GCCTCCAAGA GCTGGAGAGC 
TGTTCTGGAC TGGGCAGCAC ATCTGATGAT ACGGATGTCA GGGAGGTCAG TTCCCGCCCC 
AGCACACCAG GCCTCAGTGT TGTGTCCGGT ATAAGTGCTA CATCGGAGGA TATTCCCAAT 
AAGATCGAAG ACCTGAGATC TGAGTGCAGC TCTGATTTTG GGGGCAAAGA TTCTGTAACT 
AGTCCAGATA TGGATGACAT AGCCCATGGC GCTCATCAGC TGACCTCTCC TCCCTCTCAG 
TCAGAGTCTC TGCTTGCCAT GTTTGATCCA CTGTCCTCAC ATGAAGGGGC CTCTGCTGTG 
GTAAGGCCAA AGGTTCACTA TGCCCGACCC TCGCATCCAC CTCCGGATCC TCCAATCCTG 
GAAGGTGCAG TGGGAGGAAA TGAGGCCAGG TTGCCAAACT TTGGCTCTCA CGTTTTAACT 
GCAGCTGAAA TGGAGGCGTT CAAGCAGAGA CACTCATACC CTGAGAGATT GGTTCGCAGC 
AGAAGCTCTG ATATAGTGTC TTCTGTGCGG CGGCCCATGA GTGACCCCAG CTGGAACCGA 
CGTCCAGGGA ATGAAGAACT TCCTCCAGCT GCAGCCACTG GTGCTACTTC TTTGGTGGCT 
GCACCTCACT CGTCATCTTC GTCCCCGAGT AAGGACTCTT CTAGAGGAGA GACTGAGGAA 
CGCAAGGACA GTGACGATGA GAGGTCAGAC AGGAGCAGGC CTTGGTGGAG GAAGCGCTTT 
GTTTCAGCTA TGCCTAAAGC TCCTATACCA TTTAGAAAGA AAGAAAAACA AGAAAAAGAC 
AAAGATGATC TGGGTCCTGA CAGATTCTCA ACACTCACAG ATGAGCCCAG CCCTAGGCTC 
AGTGCTCAAG CTCAGGTTGC TGAAGATATT CTGGACAAGT ACCGGAATGC CATTAAACGG 
ACCAGCCCCA GTGAAGGAGC AATGGCAAAT GATGAGAGTG CAGAGGTCAT GGGTGATGGT 
GAAAGTGCCC ACGACTCTCC TCGTGAGGAA GCACTGCAGA ACATCTCAGC CGACGACCTC 
CCGGACTCCG CCAGCCAGGC AGCCCACCCT CAGGACTCTG CTTTCTCTTA CAGAGATGTA 
AAAAAGAAGC TGAGGCTCGC ACTTTGCTCT GCAGACTCTG TGGCCTTCCC AGTGCTAACT 
CATTCAACAA GGAATGGATT GCCAGACCAC ACAGACCCAG AAGACAATGA AATTGTATGC 
TTCTTAAAAG TTCAAATAGC TGAAGCAATT AATTTACAAG ATAAGAGCTT AATGGCTCAA 
CTTCAAGAAA CAATGCGTTG TGTGTGCCGC TTTGACAATA GGACTTGTAG GAAGTTGCTG 
GCATCCATTG CTGAAGACTA CAGAAAAAGA GCTCCGTATA TTGCTTATCT TACCCGCTGC 
CGACAAGGAC TGCAGACCAC ACAGGCTCAC TTGGAAAGGC TCTTGCAAAG AGTTTTGCGG 
GACAAAGAGG TGGCCAATCG GTACTTCACC ACTGTCTGTG TGCGGTTACT GCTTGAGAGC 
AAAGAAAAGA AAATCAGGGA GTTCATTCAA GACTTTCAGA AACTTACTGC TGCTGATGAT 
AAAACGGCTC AGGTGGAGGA TTTTCTACAG TTCCTGTATG GTGTGATGGC TCAGGATGTC 
ATATGGCAGA ATGCCAGTGA GGAGCAGCTG CAGGATGCCC AGCTGGCCAT CGAGCGGAGT 
GTGATGAATC GGATCTTCAA GCTTGCTTTC TACCCTAACC AGGATGGGGA CATACTTCGT 
GACCAGGTTC TGCATGAACA TATCCAGAGA TTATCTAAAG TAGTGACTGC AAACCACAGA 
GCTCTGCAGA TCCCTGAGGT TTATCTCCGT GAGGCACCAT GGCCATCTGC ACAGTCAGAA 
ATCAGGACAA TAAGTGCTTA CAAAACGCCC CGCGACAAAG TGCAGTGCAT CCTGAGGATG 
TGCTCCACCA TCATGAACCT CTTGAGCCTG GCCAACGAGG ACTCTGTCCC CGGGGCAGAT 
GACTTTGTTC CTGTGTTGGT GTTTGTGTTA ATAAAGGCAA ACCCACCCTG CCTGCTGTCC 
ACTGTGCAGT ACATCAGCAG CTTCTATGCC AGCTGTCTGT CTGGAGAGGA GTCCTACTGG 
TGGATGCAGT TCACAGCAGC CGTGGAGTTT ATTAAAACCA TCGATGACAG AAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAPVD1 ( XM_011239149.2 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_011237451.1 Mus musculus GTPase activating protein and VPS9 domains 1 (Gapvd1), transcript variant X6, mRNA.

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CloneID	OMu69991
Clone ID Related Accession (Same CDS sequence)	XM_011239149.2 , XM_030251955.1
Accession Version	XM_011239149.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4377bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	GTPase-activating protein and VPS9 domain-containing protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011239149.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGC TAGATATTCA CACATTGGCC CACCACCTCA AGCAGGAACG CTTATATGTG 
AGCTCTGAGA AACAGCTCAT TCAGAGGCTA AATGCCGATG TACTGAAGAC AGCTGAAAAG 
CTGTATCGAA CAGCGTGGAT TGCAAAGCAA CAGAGGATTA ATTTGGATCG TCTTATTATA 
ACCAGTGCTG AAGCTTCCCC TGCTGAGTGT TGCCAGCATG CCAAGATTTT GGAAGATACA 
CAGTTTGTTG ATGGATATAA GCAACTGGGA TTTCAGGAAA CAGCTTATGG AGAATTCCTG 
AGTCGGTTAA GAGAAAATCC ACGACTTATT GCCTCCTCTT TGGTTGCTGG AGAAAAACTC 
AATCAGGAGA ACACACAAAG TGTTATCTAC ACTGTTTTTA CCTCCTTGTA TGGCAACTGT 
ATTATGCAAG AAGATGAAAG CTACCTCCTT CAGGTTTTAC GATACTTGAT TGAATTTGAA 
CTTAAAGAAA GTGACAACCC CAGGCGCCTT TTGAGGAGAG GAACTTGTGC CTTCAGCATC 
TTATTTAAAC TTTTTTCTGA AGGACTGTTT TCTGCCAAAC TTTTCCTTAC GGCTACTTTG 
CATGAGCCAA TCATGCAGCT CCTCGTAGAG GATGAAGACC ACCTGGAAAC AGATCCAAAC 
AAGCTGATAG AGAGATTCTC TCCAGCTCAG CAAGAAAAAC TCTTTGGAGA GAAAGGCTCA 
GATAGATTTA GGCAAAAGGT TCAAGAAATG GTAGATTCCA ATGAGGCTAA ACTAGTGGCT 
TTGGTGAACA AATTTATTGG TTATCTTAAA CAGAATACAT ACTGCTTTCC ACATAGTTTG 
AGGTGGATTG TGTCACAGAT GTACAAAACT CTTTCTTGTG TAGACAGATT GGAGGTTGGG 
GAAGTCAGAG CAATGTGTAC CGATCTTCTG CTGGCTTGCT TCATTTGTCC TGCAGTTGTC 
AATCCAGAGC AGTATGGGAT AATATCTGAT GCTCCTATTA ATGAGGTGGC AAGATTTAAC 
CTGATGCAGG TTGGCCGCCT TTTGCAGCAG CTAGCAATGA CTGGCACTGA AGAAGGAGAT 
CCCCGGACCA AAAATAGCCT TGGAAAATTT GACAAAGGCT GTGTCGCTGC TTTCCTTGAT 
GTTGTAATTG GGGGCCGTGC AGTGGAGACT CCGCCAATGT CCTCTGTTAA TCTTCTGGAA 
GGCTTGAGCA GAACTGTGGT GTATATAAGT TACAGTCAGC TTATTACTCT GGTAAATTTT 
ATGAAGAGTG TGATGTCTGG AGATCAACTG AAAGAAGATA GAATGGCTCT TGACAATTTG 
TTGGCAAACC TGCCCCAGGC TAAGCCAGGG AAGAGCAGCA GTCTGGACAT GACTCCCTAT 
AGCACTCCTC AGATGTCTCC AGCAACCACT CCAGCCAACA AGAAGAATCG ATTACCTATA 
GGACAACAGT TAGCAGCCAT CACTGCCTGG GATTCGTCAG CCACCAATCT TACTGCTCAT 
ATTCCTCTAG TAACCCCATT TGCAACTCGG AGCAGAAGCC GAAGTAATAT GTTAATGGAC 
CTACATATGG ATCATGAAGG ATCATCTCAA GAAACCATCC AGGAGGTACA GCCAGAAGAG 
GTGCTGGTCA TTTCCTTAGG GACAGGCCCT CAGCTCACTC CAGGGATGAT GTCAGAAAAT 
GAGGTTCTAA ACATGCAGCT TTCGGATGGA GGACAAGGAG ATGTCCCTGT TGATGAAAAC 
AAACTCCACG GTAAACCTGA TAAAACCTTG CGCTTTTCCC TCTGCAGTGA TAATCTGGAA 
GGCATATCTG AAGGTCCTTC CAATCGCTCC AATTCGGTGT CTTCTCTCGA CCTGGAGGGA 
GAGTCTGTAT CTGAACTTGG GGCAGGACCT TCTGGGAGTA ACGGAGTTGA AGCACTGCAG 
CTGTTAGAGC ACGAGCAAGC TACAACACAA GATAATCTTG ATGATAAACT GAGGAAGTTT 
GAGATTCGTG ACATGATGGG ACTAACAGAT GACAGGGACA TATCCGAGAC TGTGAGTGAG 
ACCTGGAGCA CTGATGTCCT GGGAAGTGAC TTCGACCCGA ACGTTGATGA AGATCGTTTG 
CAAGAGATTG CAGGTGCAGC AGCAGAGAAC GTGTTAGGTA GTTTGCTGTG CCTTCCAGGT 
TCAGGGTCAG TTCTCCTTGA CCCTTGCACT GGCTCTACCA TATCAGAGAC CACAAGTGAA 
GCTTGGAGTG TAGAGGTATT GCCAAGTGAC TCAGAGGCTC CAGACTTAAA GCAGGAAGAG 
CGCCTCCAAG AGCTGGAGAG CTGTTCTGGA CTGGGCAGCA CATCTGATGA TACGGATGTC 
AGGGAGGTCA GTTCCCGCCC CAGCACACCA GGCCTCAGTG TTGTGTCCGG TATAAGTGCT 
ACATCGGAGG ATATTCCCAA TAAGATCGAA GACCTGAGAT CTGAGTGCAG CTCTGATTTT 
GGGGGCAAAG ATTCTGTAAC TAGTCCAGAT ATGGATGACA TAGCCCATGG CGCTCATCAG 
CTGACCTCTC CTCCCTCTCA GTCAGAGTCT CTGCTTGCCA TGTTTGATCC ACTGTCCTCA 
CATGAAGGGG CCTCTGCTGT GGTAAGGCCA AAGGTTCACT ATGCCCGACC CTCGCATCCA 
CCTCCGGATC CTCCAATCCT GGAAGGTGCA GTGGGAGGAA ATGAGGCCAG GTTGCCAAAC 
TTTGGCTCTC ACGTTTTAAC TGCAGCTGAA ATGGAGGCGT TCAAGCAGAG ACACTCATAC 
CCTGAGAGAT TGGTTCGCAG CAGAAGCTCT GATATAGTGT CTTCTGTGCG GCGGCCCATG 
AGTGACCCCA GCTGGAACCG ACGTCCAGGG AATGAAGAAC TTCCTCCAGC TGCAGCCACT 
GGTGCTACTT CTTTGGTGGC TGCACCTCAC TCGTCATCTT CGTCCCCGAG TAAGGACTCT 
TCTAGAGGAG AGACTGAGGA ACGCAAGGAC AGTGACGATG AGAGGTCAGA CAGGAGCAGG 
CCTTGGTGGA GGAAGCGCTT TGTTTCAGCT ATGCCTAAAG ATGAGCCCAG CCCTAGGCTC 
AGTGCTCAAG CTCAGGTTGC TGAAGATATT CTGGACAAGT ACCGGAATGC CATTAAACGG 
ACCAGCCCCA GTGAAGGAGC AATGGCAAAT GATGAGAGTG CAGAGGTCAT GGGTGATGGT 
GAAAGTGCCC ACGACTCTCC TCGTGAGGAA GCACTGCAGA ACATCTCAGC CGACGACCTC 
CCGGACTCCG CCAGCCAGGC AGCCCACCCT CAGGACTCTG CTTTCTCTTA CAGAGATGTA 
AAAAAGAAGC TGAGGCTCGC ACTTTGCTCT GCAGACTCTG TGGCCTTCCC AGTGCTAACT 
CATTCAACAA GGAATGGATT GCCAGACCAC ACAGACCCAG AAGACAATGA AATTGTATGC 
TTCTTAAAAG TTCAAATAGC TGAAGCAATT AATTTACAAG ATAAGAGCTT AATGGCTCAA 
CTTCAAGAAA CAATGCGTTG TGTGTGCCGC TTTGACAATA GGACTTGTAG GAAGTTGCTG 
GCATCCATTG CTGAAGACTA CAGAAAAAGA GCTCCGTATA TTGCTTATCT TACCCGCTGC 
CGACAAGGAC TGCAGACCAC ACAGGCTCAC TTGGAAAGGC TCTTGCAAAG AGTTTTGCGG 
GACAAAGAGG TGGCCAATCG GTACTTCACC ACTGTCTGTG TGCGGTTACT GCTTGAGAGC 
AAAGAAAAGA AAATCAGGGA GTTCATTCAA GACTTTCAGA AACTTACTGC TGCTGATGAT 
AAAACGGCTC AGGTGGAGGA TTTTCTACAG TTCCTGTATG GTGTGATGGC TCAGGATGTC 
ATATGGCAGA ATGCCAGTGA GGAGCAGCTG CAGGATGCCC AGCTGGCCAT CGAGCGGAGT 
GTGATGAATC GGATCTTCAA GCTTGCTTTC TACCCTAACC AGGATGGGGA CATACTTCGT 
GACCAGGTTC TGCATGAACA TATCCAGAGA TTATCTAAAG TAGTGACTGC AAACCACAGA 
GCTCTGCAGA TCCCTGAGGT TTATCTCCGT GAGGCACCAT GGCCATCTGC ACAGTCAGAA 
ATCAGGACAA TAAGTGCTTA CAAAACGCCC CGCGACAAAG TGCAGTGCAT CCTGAGGATG 
TGCTCCACCA TCATGAACCT CTTGAGCCTG GCCAACGAGG ACTCTGTCCC CGGGGCAGAT 
GACTTTGTTC CTGTGTTGGT GTTTGTGTTA ATAAAGGCAA ACCCACCCTG CCTGCTGTCC 
ACTGTGCAGT ACATCAGCAG CTTCTATGCC AGCTGTCTGT CTGGAGAGGA GTCCTACTGG 
TGGATGCAGT TCACAGCAGC CGTGGAGTTT ATTAAAACCA TCGATGACAG AAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011237451.1
CDS	593..4969
Translation	MVKLDIHTLAHHLKQERLYVSSEKQLIQRLNADVLKTAEKLYRTAWIAKQQRINLDRLIITSAEASPAECCQHAKILEDTQFVDGYKQLGFQETAYGEFLSRLRENPRLIASSLVAGEKLNQENTQSVIYTVFTSLYGNCIMQEDESYLLQVLRYLIEFELKESDNPRRLLRRGTCAFSILFKLFSEGLFSAKLFLTATLHEPIMQLLVEDEDHLETDPNKLIERFSPAQQEKLFGEKGSDRFRQKVQEMVDSNEAKLVALVNKFIGYLKQNTYCFPHSLRWIVSQMYKTLSCVDRLEVGEVRAMCTDLLLACFICPAVVNPEQYGIISDAPINEVARFNLMQVGRLLQQLAMTGTEEGDPRTKNSLGKFDKGCVAAFLDVVIGGRAVETPPMSSVNLLEGLSRTVVYISYSQLITLVNFMKSVMSGDQLKEDRMALDNLLANLPQAKPGKSSSLDMTPYSTPQMSPATTPANKKNRLPIGQQLAAITAWDSSATNLTAHIPLVTPFATRSRSRSNMLMDLHMDHEGSSQETIQEVQPEEVLVISLGTGPQLTPGMMSENEVLNMQLSDGGQGDVPVDENKLHGKPDKTLRFSLCSDNLEGISEGPSNRSNSVSSLDLEGESVSELGAGPSGSNGVEALQLLEHEQATTQDNLDDKLRKFEIRDMMGLTDDRDISETVSETWSTDVLGSDFDPNVDEDRLQEIAGAAAENVLGSLLCLPGSGSVLLDPCTGSTISETTSEAWSVEVLPSDSEAPDLKQEERLQELESCSGLGSTSDDTDVREVSSRPSTPGLSVVSGISATSEDIPNKIEDLRSECSSDFGGKDSVTSPDMDDIAHGAHQLTSPPSQSESLLAMFDPLSSHEGASAVVRPKVHYARPSHPPPDPPILEGAVGGNEARLPNFGSHVLTAAEMEAFKQRHSYPERLVRSRSSDIVSSVRRPMSDPSWNRRPGNEELPPAAATGATSLVAAPHSSSSSPSKDSSRGETEERKDSDDERSDRSRPWWRKRFVSAMPKDEPSPRLSAQAQVAEDILDKYRNAIKRTSPSEGAMANDESAEVMGDGESAHDSPREEALQNISADDLPDSASQAAHPQDSAFSYRDVKKKLRLALCSADSVAFPVLTHSTRNGLPDHTDPEDNEIVCFLKVQIAEAINLQDKSLMAQLQETMRCVCRFDNRTCRKLLASIAEDYRKRAPYIAYLTRCRQGLQTTQAHLERLLQRVLRDKEVANRYFTTVCVRLLLESKEKKIREFIQDFQKLTAADDKTAQVEDFLQFLYGVMAQDVIWQNASEEQLQDAQLAIERSVMNRIFKLAFYPNQDGDILRDQVLHEHIQRLSKVVTANHRALQIPEVYLREAPWPSAQSEIRTISAYKTPRDKVQCILRMCSTIMNLLSLANEDSVPGADDFVPVLVFVLIKANPPCLLSTVQYISSFYASCLSGEESYWWMQFTAAVEFIKTIDDRK

Target ORF information:

RefSeq Version	XM_011239149.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus GTPase activating protein and VPS9 domains 1 (Gapvd1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011239149.2

ORF Insert Sequence:

ATGGTGAAGC TAGATATTCA CACATTGGCC CACCACCTCA AGCAGGAACG CTTATATGTG 
AGCTCTGAGA AACAGCTCAT TCAGAGGCTA AATGCCGATG TACTGAAGAC AGCTGAAAAG 
CTGTATCGAA CAGCGTGGAT TGCAAAGCAA CAGAGGATTA ATTTGGATCG TCTTATTATA 
ACCAGTGCTG AAGCTTCCCC TGCTGAGTGT TGCCAGCATG CCAAGATTTT GGAAGATACA 
CAGTTTGTTG ATGGATATAA GCAACTGGGA TTTCAGGAAA CAGCTTATGG AGAATTCCTG 
AGTCGGTTAA GAGAAAATCC ACGACTTATT GCCTCCTCTT TGGTTGCTGG AGAAAAACTC 
AATCAGGAGA ACACACAAAG TGTTATCTAC ACTGTTTTTA CCTCCTTGTA TGGCAACTGT 
ATTATGCAAG AAGATGAAAG CTACCTCCTT CAGGTTTTAC GATACTTGAT TGAATTTGAA 
CTTAAAGAAA GTGACAACCC CAGGCGCCTT TTGAGGAGAG GAACTTGTGC CTTCAGCATC 
TTATTTAAAC TTTTTTCTGA AGGACTGTTT TCTGCCAAAC TTTTCCTTAC GGCTACTTTG 
CATGAGCCAA TCATGCAGCT CCTCGTAGAG GATGAAGACC ACCTGGAAAC AGATCCAAAC 
AAGCTGATAG AGAGATTCTC TCCAGCTCAG CAAGAAAAAC TCTTTGGAGA GAAAGGCTCA 
GATAGATTTA GGCAAAAGGT TCAAGAAATG GTAGATTCCA ATGAGGCTAA ACTAGTGGCT 
TTGGTGAACA AATTTATTGG TTATCTTAAA CAGAATACAT ACTGCTTTCC ACATAGTTTG 
AGGTGGATTG TGTCACAGAT GTACAAAACT CTTTCTTGTG TAGACAGATT GGAGGTTGGG 
GAAGTCAGAG CAATGTGTAC CGATCTTCTG CTGGCTTGCT TCATTTGTCC TGCAGTTGTC 
AATCCAGAGC AGTATGGGAT AATATCTGAT GCTCCTATTA ATGAGGTGGC AAGATTTAAC 
CTGATGCAGG TTGGCCGCCT TTTGCAGCAG CTAGCAATGA CTGGCACTGA AGAAGGAGAT 
CCCCGGACCA AAAATAGCCT TGGAAAATTT GACAAAGGCT GTGTCGCTGC TTTCCTTGAT 
GTTGTAATTG GGGGCCGTGC AGTGGAGACT CCGCCAATGT CCTCTGTTAA TCTTCTGGAA 
GGCTTGAGCA GAACTGTGGT GTATATAAGT TACAGTCAGC TTATTACTCT GGTAAATTTT 
ATGAAGAGTG TGATGTCTGG AGATCAACTG AAAGAAGATA GAATGGCTCT TGACAATTTG 
TTGGCAAACC TGCCCCAGGC TAAGCCAGGG AAGAGCAGCA GTCTGGACAT GACTCCCTAT 
AGCACTCCTC AGATGTCTCC AGCAACCACT CCAGCCAACA AGAAGAATCG ATTACCTATA 
GGACAACAGT TAGCAGCCAT CACTGCCTGG GATTCGTCAG CCACCAATCT TACTGCTCAT 
ATTCCTCTAG TAACCCCATT TGCAACTCGG AGCAGAAGCC GAAGTAATAT GTTAATGGAC 
CTACATATGG ATCATGAAGG ATCATCTCAA GAAACCATCC AGGAGGTACA GCCAGAAGAG 
GTGCTGGTCA TTTCCTTAGG GACAGGCCCT CAGCTCACTC CAGGGATGAT GTCAGAAAAT 
GAGGTTCTAA ACATGCAGCT TTCGGATGGA GGACAAGGAG ATGTCCCTGT TGATGAAAAC 
AAACTCCACG GTAAACCTGA TAAAACCTTG CGCTTTTCCC TCTGCAGTGA TAATCTGGAA 
GGCATATCTG AAGGTCCTTC CAATCGCTCC AATTCGGTGT CTTCTCTCGA CCTGGAGGGA 
GAGTCTGTAT CTGAACTTGG GGCAGGACCT TCTGGGAGTA ACGGAGTTGA AGCACTGCAG 
CTGTTAGAGC ACGAGCAAGC TACAACACAA GATAATCTTG ATGATAAACT GAGGAAGTTT 
GAGATTCGTG ACATGATGGG ACTAACAGAT GACAGGGACA TATCCGAGAC TGTGAGTGAG 
ACCTGGAGCA CTGATGTCCT GGGAAGTGAC TTCGACCCGA ACGTTGATGA AGATCGTTTG 
CAAGAGATTG CAGGTGCAGC AGCAGAGAAC GTGTTAGGTA GTTTGCTGTG CCTTCCAGGT 
TCAGGGTCAG TTCTCCTTGA CCCTTGCACT GGCTCTACCA TATCAGAGAC CACAAGTGAA 
GCTTGGAGTG TAGAGGTATT GCCAAGTGAC TCAGAGGCTC CAGACTTAAA GCAGGAAGAG 
CGCCTCCAAG AGCTGGAGAG CTGTTCTGGA CTGGGCAGCA CATCTGATGA TACGGATGTC 
AGGGAGGTCA GTTCCCGCCC CAGCACACCA GGCCTCAGTG TTGTGTCCGG TATAAGTGCT 
ACATCGGAGG ATATTCCCAA TAAGATCGAA GACCTGAGAT CTGAGTGCAG CTCTGATTTT 
GGGGGCAAAG ATTCTGTAAC TAGTCCAGAT ATGGATGACA TAGCCCATGG CGCTCATCAG 
CTGACCTCTC CTCCCTCTCA GTCAGAGTCT CTGCTTGCCA TGTTTGATCC ACTGTCCTCA 
CATGAAGGGG CCTCTGCTGT GGTAAGGCCA AAGGTTCACT ATGCCCGACC CTCGCATCCA 
CCTCCGGATC CTCCAATCCT GGAAGGTGCA GTGGGAGGAA ATGAGGCCAG GTTGCCAAAC 
TTTGGCTCTC ACGTTTTAAC TGCAGCTGAA ATGGAGGCGT TCAAGCAGAG ACACTCATAC 
CCTGAGAGAT TGGTTCGCAG CAGAAGCTCT GATATAGTGT CTTCTGTGCG GCGGCCCATG 
AGTGACCCCA GCTGGAACCG ACGTCCAGGG AATGAAGAAC TTCCTCCAGC TGCAGCCACT 
GGTGCTACTT CTTTGGTGGC TGCACCTCAC TCGTCATCTT CGTCCCCGAG TAAGGACTCT 
TCTAGAGGAG AGACTGAGGA ACGCAAGGAC AGTGACGATG AGAGGTCAGA CAGGAGCAGG 
CCTTGGTGGA GGAAGCGCTT TGTTTCAGCT ATGCCTAAAG ATGAGCCCAG CCCTAGGCTC 
AGTGCTCAAG CTCAGGTTGC TGAAGATATT CTGGACAAGT ACCGGAATGC CATTAAACGG 
ACCAGCCCCA GTGAAGGAGC AATGGCAAAT GATGAGAGTG CAGAGGTCAT GGGTGATGGT 
GAAAGTGCCC ACGACTCTCC TCGTGAGGAA GCACTGCAGA ACATCTCAGC CGACGACCTC 
CCGGACTCCG CCAGCCAGGC AGCCCACCCT CAGGACTCTG CTTTCTCTTA CAGAGATGTA 
AAAAAGAAGC TGAGGCTCGC ACTTTGCTCT GCAGACTCTG TGGCCTTCCC AGTGCTAACT 
CATTCAACAA GGAATGGATT GCCAGACCAC ACAGACCCAG AAGACAATGA AATTGTATGC 
TTCTTAAAAG TTCAAATAGC TGAAGCAATT AATTTACAAG ATAAGAGCTT AATGGCTCAA 
CTTCAAGAAA CAATGCGTTG TGTGTGCCGC TTTGACAATA GGACTTGTAG GAAGTTGCTG 
GCATCCATTG CTGAAGACTA CAGAAAAAGA GCTCCGTATA TTGCTTATCT TACCCGCTGC 
CGACAAGGAC TGCAGACCAC ACAGGCTCAC TTGGAAAGGC TCTTGCAAAG AGTTTTGCGG 
GACAAAGAGG TGGCCAATCG GTACTTCACC ACTGTCTGTG TGCGGTTACT GCTTGAGAGC 
AAAGAAAAGA AAATCAGGGA GTTCATTCAA GACTTTCAGA AACTTACTGC TGCTGATGAT 
AAAACGGCTC AGGTGGAGGA TTTTCTACAG TTCCTGTATG GTGTGATGGC TCAGGATGTC 
ATATGGCAGA ATGCCAGTGA GGAGCAGCTG CAGGATGCCC AGCTGGCCAT CGAGCGGAGT 
GTGATGAATC GGATCTTCAA GCTTGCTTTC TACCCTAACC AGGATGGGGA CATACTTCGT 
GACCAGGTTC TGCATGAACA TATCCAGAGA TTATCTAAAG TAGTGACTGC AAACCACAGA 
GCTCTGCAGA TCCCTGAGGT TTATCTCCGT GAGGCACCAT GGCCATCTGC ACAGTCAGAA 
ATCAGGACAA TAAGTGCTTA CAAAACGCCC CGCGACAAAG TGCAGTGCAT CCTGAGGATG 
TGCTCCACCA TCATGAACCT CTTGAGCCTG GCCAACGAGG ACTCTGTCCC CGGGGCAGAT 
GACTTTGTTC CTGTGTTGGT GTTTGTGTTA ATAAAGGCAA ACCCACCCTG CCTGCTGTCC 
ACTGTGCAGT ACATCAGCAG CTTCTATGCC AGCTGTCTGT CTGGAGAGGA GTCCTACTGG 
TGGATGCAGT TCACAGCAGC CGTGGAGTTT ATTAAAACCA TCGATGACAG AAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAPVD1 ( XM_011239150.2 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_011237452.1 Mus musculus GTPase activating protein and VPS9 domains 1 (Gapvd1), transcript variant X7, mRNA.

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CloneID	OMu69992
Clone ID Related Accession (Same CDS sequence)	XM_011239150.3 , XM_011239150.2
Accession Version	XM_011239150.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	GTPase-activating protein and VPS9 domain-containing protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011239150.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGC TAGATATTCA CACATTGGCC CACCACCTCA AGCAGGAACG CTTATATGTG 
AGCTCTGAGA AACAGCTCAT TCAGAGGCTA AATGCCGATG TACTGAAGAC AGCTGAAAAG 
CTGTATCGAA CAGCGTGGAT TGCAAAGCAA CAGAGGATTA ATTTGGATCG TCTTATTATA 
ACCAGTGCTG AAGCTTCCCC TGCTGAGTGT TGCCAGCATG CCAAGATTTT GGAAGATACA 
CAGTTTGTTG ATGGATATAA GCAACTGGGA TTTCAGGAAA CAGCTTATGG AGAATTCCTG 
AGTCGGTTAA GAGAAAATCC ACGACTTATT GCCTCCTCTT TGGTTGCTGG AGAAAAACTC 
AATCAGGAGA ACACACAAAG TGTTATCTAC ACTGTTTTTA CCTCCTTGTA TGGCAACTGT 
ATTATGCAAG AAGATGAAAG CTACCTCCTT CAGGTTTTAC GATACTTGAT TGAATTTGAA 
CTTAAAGAAA GTGACAACCC CAGGCGCCTT TTGAGGAGAG GAACTTGTGC CTTCAGCATC 
TTATTTAAAC TTTTTTCTGA AGGACTGTTT TCTGCCAAAC TTTTCCTTAC GGCTACTTTG 
CATGAGCCAA TCATGCAGCT CCTCGTAGAG GATGAAGACC ACCTGGAAAC AGATCCAAAC 
AAGCTGATAG AGAGATTCTC TCCAGCTCAG CAAGAAAAAC TCTTTGGAGA GAAAGGCTCA 
GATAGATTTA GGCAAAAGGT TCAAGAAATG GTAGATTCCA ATGAGGCTAA ACTAGTGGCT 
TTGGTGAACA AATTTATTGG TTATCTTAAA CAGAATACAT ACTGCTTTCC ACATAGTTTG 
AGGTGGATTG TGTCACAGAT GTACAAAACT CTTTCTTGTG TAGACAGATT GGAGGTTGGG 
GAAGTCAGAG CAATGTGTAC CGATCTTCTG CTGGCTTGCT TCATTTGTCC TGCAGTTGTC 
AATCCAGAGC AGTATGGGAT AATATCTGAT GCTCCTATTA ATGAGGTGGC AAGATTTAAC 
CTGATGCAGG TTGGCCGCCT TTTGCAGCAG CTAGCAATGA CTGGCACTGA AGAAGGAGAT 
CCCCGGACCA AAAATAGCCT TGGAAAATTT GACAAAGGCT GTGTCGCTGC TTTCCTTGAT 
GTTGTAATTG GGGGCCGTGC AGTGGAGACT CCGCCAATGT CCTCTGTTAA TCTTCTGGAA 
GGCTTGAGCA GAACTGTGGT GTATATAAGT TACAGTCAGC TTATTACTCT GGTAAATTTT 
ATGAAGAGTG TGATGTCTGG AGATCAACTG AAAGAAGATA GAATGGCTCT TGACAATTTG 
TTGGCAAACC TGCCCCAGGC TAAGCCAGGG AAGAGCAGCA GTCTGGACAT GACTCCCTAT 
AGCACTCCTC AGATGTCTCC AGCAACCACT CCAGCCAACA AGAAGAATCG ATTACCTATA 
GCAACTCGGA GCAGAAGCCG AAGTAATATG TTAATGGACC TACATATGGA TCATGAAGGA 
TCATCTCAAG AAACCATCCA GGAGGTACAG CCAGAAGAGG TGCTGGTCAT TTCCTTAGGG 
ACAGGCCCTC AGCTCACTCC AGGGATGATG TCAGAAAATG AGGTTCTAAA CATGCAGCTT 
TCGGATGGAG GACAAGGAGA TGTCCCTGTT GATGAAAACA AACTCCACGG TAAACCTGAT 
AAAACCTTGC GCTTTTCCCT CTGCAGTGAT AATCTGGAAG GCATATCTGA AGGTCCTTCC 
AATCGCTCCA ATTCGGTGTC TTCTCTCGAC CTGGAGGGAG AGTCTGTATC TGAACTTGGG 
GCAGGACCTT CTGGGAGTAA CGGAGTTGAA GCACTGCAGC TGTTAGAGCA CGAGCAAGCT 
ACAACACAAG ATAATCTTGA TGATAAACTG AGGAAGTTTG AGATTCGTGA CATGATGGGA 
CTAACAGATG ACAGGGACAT ATCCGAGACT GTGAGTGAGA CCTGGAGCAC TGATGTCCTG 
GGAAGTGACT TCGACCCGAA CGTTGATGAA GATCGTTTGC AAGAGATTGC AGGTGCAGCA 
GCAGAGAACG TGTTAGGTAG TTTGCTGTGC CTTCCAGGTT CAGGGTCAGT TCTCCTTGAC 
CCTTGCACTG GCTCTACCAT ATCAGAGACC ACAAGTGAAG CTTGGAGTGT AGAGGTATTG 
CCAAGTGACT CAGAGGCTCC AGACTTAAAG CAGGAAGAGC GCCTCCAAGA GCTGGAGAGC 
TGTTCTGGAC TGGGCAGCAC ATCTGATGAT ACGGATGTCA GGGAGGTCAG TTCCCGCCCC 
AGCACACCAG GCCTCAGTGT TGTGTCCGGT ATAAGTGCTA CATCGGAGGA TATTCCCAAT 
AAGATCGAAG ACCTGAGATC TGAGTGCAGC TCTGATTTTG GGGGCAAAGA TTCTGTAACT 
AGTCCAGATA TGGATGACAT AGCCCATGGC GCTCATCAGC TGACCTCTCC TCCCTCTCAG 
TCAGAGTCTC TGCTTGCCAT GTTTGATCCA CTGTCCTCAC ATGAAGGGGC CTCTGCTGTG 
GTAAGGCCAA AGGTTCACTA TGCCCGACCC TCGCATCCAC CTCCGGATCC TCCAATCCTG 
GAAGGTGCAG TGGGAGGAAA TGAGGCCAGG TTGCCAAACT TTGGCTCTCA CGTTTTAACT 
GCAGCTGAAA TGGAGGCGTT CAAGCAGAGA CACTCATACC CTGAGAGATT GGTTCGCAGC 
AGAAGCTCTG ATATAGTGTC TTCTGTGCGG CGGCCCATGA GTGACCCCAG CTGGAACCGA 
CGTCCAGGGA ATGAAGAACT TCCTCCAGCT GCAGCCACTG GTGCTACTTC TTTGGTGGCT 
GCACCTCACT CGTCATCTTC GTCCCCGAGT AAGGACTCTT CTAGAGGAGA GACTGAGGAA 
CGCAAGGACA GTGACGATGA GAGGTCAGAC AGGAGCAGGC CTTGGTGGAG GAAGCGCTTT 
GTTTCAGCTA TGCCTAAAGA TGAGCCCAGC CCTAGGCTCA GTGCTCAAGC TCAGGTTGCT 
GAAGATATTC TGGACAAGTA CCGGAATGCC ATTAAACGGA CCAGCCCCAG TGAAGGAGCA 
ATGGCAAATG ATGAGAGTGC AGAGGTCATG GGTGATGGTG AAAGTGCCCA CGACTCTCCT 
CGTGAGGAAG CACTGCAGAA CATCTCAGCC GACGACCTCC CGGACTCCGC CAGCCAGGCA 
GCCCACCCTC AGGACTCTGC TTTCTCTTAC AGAGATGTAA AAAAGAAGCT GAGGCTCGCA 
CTTTGCTCTG CAGACTCTGT GGCCTTCCCA GTGCTAACTC ATTCAACAAG GAATGGATTG 
CCAGACCACA CAGACCCAGA AGACAATGAA ATTGTATGCT TCTTAAAAGT TCAAATAGCT 
GAAGCAATTA ATTTACAAGA TAAGAGCTTA ATGGCTCAAC TTCAAGAAAC AATGCGTTGT 
GTGTGCCGCT TTGACAATAG GACTTGTAGG AAGTTGCTGG CATCCATTGC TGAAGACTAC 
AGAAAAAGAG CTCCGTATAT TGCTTATCTT ACCCGCTGCC GACAAGGACT GCAGACCACA 
CAGGCTCACT TGGAAAGGCT CTTGCAAAGA GTTTTGCGGG ACAAAGAGGT GGCCAATCGG 
TACTTCACCA CTGTCTGTGT GCGGTTACTG CTTGAGAGCA AAGAAAAGAA AATCAGGGAG 
TTCATTCAAG ACTTTCAGAA ACTTACTGCT GCTGATGATA AAACGGCTCA GGTGGAGGAT 
TTTCTACAGT TCCTGTATGG TGTGATGGCT CAGGATGTCA TATGGCAGAA TGCCAGTGAG 
GAGCAGCTGC AGGATGCCCA GCTGGCCATC GAGCGGAGTG TGATGAATCG GATCTTCAAG 
CTTGCTTTCT ACCCTAACCA GGATGGGGAC ATACTTCGTG ACCAGGTTCT GCATGAACAT 
ATCCAGAGAT TATCTAAAGT AGTGACTGCA AACCACAGAG CTCTGCAGAT CCCTGAGGTT 
TATCTCCGTG AGGCACCATG GCCATCTGCA CAGTCAGAAA TCAGGACAAT AAGTGCTTAC 
AAAACGCCCC GCGACAAAGT GCAGTGCATC CTGAGGATGT GCTCCACCAT CATGAACCTC 
TTGAGCCTGG CCAACGAGGA CTCTGTCCCC GGGGCAGATG ACTTTGTTCC TGTGTTGGTG 
TTTGTGTTAA TAAAGGCAAA CCCACCCTGC CTGCTGTCCA CTGTGCAGTA CATCAGCAGC 
TTCTATGCCA GCTGTCTGTC TGGAGAGGAG TCCTACTGGT GGATGCAGTT CACAGCAGCC 
GTGGAGTTTA TTAAAACCAT CGATGACAGA AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011237452.1
CDS	592..4887
Translation	MVKLDIHTLAHHLKQERLYVSSEKQLIQRLNADVLKTAEKLYRTAWIAKQQRINLDRLIITSAEASPAECCQHAKILEDTQFVDGYKQLGFQETAYGEFLSRLRENPRLIASSLVAGEKLNQENTQSVIYTVFTSLYGNCIMQEDESYLLQVLRYLIEFELKESDNPRRLLRRGTCAFSILFKLFSEGLFSAKLFLTATLHEPIMQLLVEDEDHLETDPNKLIERFSPAQQEKLFGEKGSDRFRQKVQEMVDSNEAKLVALVNKFIGYLKQNTYCFPHSLRWIVSQMYKTLSCVDRLEVGEVRAMCTDLLLACFICPAVVNPEQYGIISDAPINEVARFNLMQVGRLLQQLAMTGTEEGDPRTKNSLGKFDKGCVAAFLDVVIGGRAVETPPMSSVNLLEGLSRTVVYISYSQLITLVNFMKSVMSGDQLKEDRMALDNLLANLPQAKPGKSSSLDMTPYSTPQMSPATTPANKKNRLPIATRSRSRSNMLMDLHMDHEGSSQETIQEVQPEEVLVISLGTGPQLTPGMMSENEVLNMQLSDGGQGDVPVDENKLHGKPDKTLRFSLCSDNLEGISEGPSNRSNSVSSLDLEGESVSELGAGPSGSNGVEALQLLEHEQATTQDNLDDKLRKFEIRDMMGLTDDRDISETVSETWSTDVLGSDFDPNVDEDRLQEIAGAAAENVLGSLLCLPGSGSVLLDPCTGSTISETTSEAWSVEVLPSDSEAPDLKQEERLQELESCSGLGSTSDDTDVREVSSRPSTPGLSVVSGISATSEDIPNKIEDLRSECSSDFGGKDSVTSPDMDDIAHGAHQLTSPPSQSESLLAMFDPLSSHEGASAVVRPKVHYARPSHPPPDPPILEGAVGGNEARLPNFGSHVLTAAEMEAFKQRHSYPERLVRSRSSDIVSSVRRPMSDPSWNRRPGNEELPPAAATGATSLVAAPHSSSSSPSKDSSRGETEERKDSDDERSDRSRPWWRKRFVSAMPKDEPSPRLSAQAQVAEDILDKYRNAIKRTSPSEGAMANDESAEVMGDGESAHDSPREEALQNISADDLPDSASQAAHPQDSAFSYRDVKKKLRLALCSADSVAFPVLTHSTRNGLPDHTDPEDNEIVCFLKVQIAEAINLQDKSLMAQLQETMRCVCRFDNRTCRKLLASIAEDYRKRAPYIAYLTRCRQGLQTTQAHLERLLQRVLRDKEVANRYFTTVCVRLLLESKEKKIREFIQDFQKLTAADDKTAQVEDFLQFLYGVMAQDVIWQNASEEQLQDAQLAIERSVMNRIFKLAFYPNQDGDILRDQVLHEHIQRLSKVVTANHRALQIPEVYLREAPWPSAQSEIRTISAYKTPRDKVQCILRMCSTIMNLLSLANEDSVPGADDFVPVLVFVLIKANPPCLLSTVQYISSFYASCLSGEESYWWMQFTAAVEFIKTIDDRK

Target ORF information:

RefSeq Version	XM_011239150.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus GTPase activating protein and VPS9 domains 1 (Gapvd1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011239150.2

ORF Insert Sequence:

ATGGTGAAGC TAGATATTCA CACATTGGCC CACCACCTCA AGCAGGAACG CTTATATGTG 
AGCTCTGAGA AACAGCTCAT TCAGAGGCTA AATGCCGATG TACTGAAGAC AGCTGAAAAG 
CTGTATCGAA CAGCGTGGAT TGCAAAGCAA CAGAGGATTA ATTTGGATCG TCTTATTATA 
ACCAGTGCTG AAGCTTCCCC TGCTGAGTGT TGCCAGCATG CCAAGATTTT GGAAGATACA 
CAGTTTGTTG ATGGATATAA GCAACTGGGA TTTCAGGAAA CAGCTTATGG AGAATTCCTG 
AGTCGGTTAA GAGAAAATCC ACGACTTATT GCCTCCTCTT TGGTTGCTGG AGAAAAACTC 
AATCAGGAGA ACACACAAAG TGTTATCTAC ACTGTTTTTA CCTCCTTGTA TGGCAACTGT 
ATTATGCAAG AAGATGAAAG CTACCTCCTT CAGGTTTTAC GATACTTGAT TGAATTTGAA 
CTTAAAGAAA GTGACAACCC CAGGCGCCTT TTGAGGAGAG GAACTTGTGC CTTCAGCATC 
TTATTTAAAC TTTTTTCTGA AGGACTGTTT TCTGCCAAAC TTTTCCTTAC GGCTACTTTG 
CATGAGCCAA TCATGCAGCT CCTCGTAGAG GATGAAGACC ACCTGGAAAC AGATCCAAAC 
AAGCTGATAG AGAGATTCTC TCCAGCTCAG CAAGAAAAAC TCTTTGGAGA GAAAGGCTCA 
GATAGATTTA GGCAAAAGGT TCAAGAAATG GTAGATTCCA ATGAGGCTAA ACTAGTGGCT 
TTGGTGAACA AATTTATTGG TTATCTTAAA CAGAATACAT ACTGCTTTCC ACATAGTTTG 
AGGTGGATTG TGTCACAGAT GTACAAAACT CTTTCTTGTG TAGACAGATT GGAGGTTGGG 
GAAGTCAGAG CAATGTGTAC CGATCTTCTG CTGGCTTGCT TCATTTGTCC TGCAGTTGTC 
AATCCAGAGC AGTATGGGAT AATATCTGAT GCTCCTATTA ATGAGGTGGC AAGATTTAAC 
CTGATGCAGG TTGGCCGCCT TTTGCAGCAG CTAGCAATGA CTGGCACTGA AGAAGGAGAT 
CCCCGGACCA AAAATAGCCT TGGAAAATTT GACAAAGGCT GTGTCGCTGC TTTCCTTGAT 
GTTGTAATTG GGGGCCGTGC AGTGGAGACT CCGCCAATGT CCTCTGTTAA TCTTCTGGAA 
GGCTTGAGCA GAACTGTGGT GTATATAAGT TACAGTCAGC TTATTACTCT GGTAAATTTT 
ATGAAGAGTG TGATGTCTGG AGATCAACTG AAAGAAGATA GAATGGCTCT TGACAATTTG 
TTGGCAAACC TGCCCCAGGC TAAGCCAGGG AAGAGCAGCA GTCTGGACAT GACTCCCTAT 
AGCACTCCTC AGATGTCTCC AGCAACCACT CCAGCCAACA AGAAGAATCG ATTACCTATA 
GCAACTCGGA GCAGAAGCCG AAGTAATATG TTAATGGACC TACATATGGA TCATGAAGGA 
TCATCTCAAG AAACCATCCA GGAGGTACAG CCAGAAGAGG TGCTGGTCAT TTCCTTAGGG 
ACAGGCCCTC AGCTCACTCC AGGGATGATG TCAGAAAATG AGGTTCTAAA CATGCAGCTT 
TCGGATGGAG GACAAGGAGA TGTCCCTGTT GATGAAAACA AACTCCACGG TAAACCTGAT 
AAAACCTTGC GCTTTTCCCT CTGCAGTGAT AATCTGGAAG GCATATCTGA AGGTCCTTCC 
AATCGCTCCA ATTCGGTGTC TTCTCTCGAC CTGGAGGGAG AGTCTGTATC TGAACTTGGG 
GCAGGACCTT CTGGGAGTAA CGGAGTTGAA GCACTGCAGC TGTTAGAGCA CGAGCAAGCT 
ACAACACAAG ATAATCTTGA TGATAAACTG AGGAAGTTTG AGATTCGTGA CATGATGGGA 
CTAACAGATG ACAGGGACAT ATCCGAGACT GTGAGTGAGA CCTGGAGCAC TGATGTCCTG 
GGAAGTGACT TCGACCCGAA CGTTGATGAA GATCGTTTGC AAGAGATTGC AGGTGCAGCA 
GCAGAGAACG TGTTAGGTAG TTTGCTGTGC CTTCCAGGTT CAGGGTCAGT TCTCCTTGAC 
CCTTGCACTG GCTCTACCAT ATCAGAGACC ACAAGTGAAG CTTGGAGTGT AGAGGTATTG 
CCAAGTGACT CAGAGGCTCC AGACTTAAAG CAGGAAGAGC GCCTCCAAGA GCTGGAGAGC 
TGTTCTGGAC TGGGCAGCAC ATCTGATGAT ACGGATGTCA GGGAGGTCAG TTCCCGCCCC 
AGCACACCAG GCCTCAGTGT TGTGTCCGGT ATAAGTGCTA CATCGGAGGA TATTCCCAAT 
AAGATCGAAG ACCTGAGATC TGAGTGCAGC TCTGATTTTG GGGGCAAAGA TTCTGTAACT 
AGTCCAGATA TGGATGACAT AGCCCATGGC GCTCATCAGC TGACCTCTCC TCCCTCTCAG 
TCAGAGTCTC TGCTTGCCAT GTTTGATCCA CTGTCCTCAC ATGAAGGGGC CTCTGCTGTG 
GTAAGGCCAA AGGTTCACTA TGCCCGACCC TCGCATCCAC CTCCGGATCC TCCAATCCTG 
GAAGGTGCAG TGGGAGGAAA TGAGGCCAGG TTGCCAAACT TTGGCTCTCA CGTTTTAACT 
GCAGCTGAAA TGGAGGCGTT CAAGCAGAGA CACTCATACC CTGAGAGATT GGTTCGCAGC 
AGAAGCTCTG ATATAGTGTC TTCTGTGCGG CGGCCCATGA GTGACCCCAG CTGGAACCGA 
CGTCCAGGGA ATGAAGAACT TCCTCCAGCT GCAGCCACTG GTGCTACTTC TTTGGTGGCT 
GCACCTCACT CGTCATCTTC GTCCCCGAGT AAGGACTCTT CTAGAGGAGA GACTGAGGAA 
CGCAAGGACA GTGACGATGA GAGGTCAGAC AGGAGCAGGC CTTGGTGGAG GAAGCGCTTT 
GTTTCAGCTA TGCCTAAAGA TGAGCCCAGC CCTAGGCTCA GTGCTCAAGC TCAGGTTGCT 
GAAGATATTC TGGACAAGTA CCGGAATGCC ATTAAACGGA CCAGCCCCAG TGAAGGAGCA 
ATGGCAAATG ATGAGAGTGC AGAGGTCATG GGTGATGGTG AAAGTGCCCA CGACTCTCCT 
CGTGAGGAAG CACTGCAGAA CATCTCAGCC GACGACCTCC CGGACTCCGC CAGCCAGGCA 
GCCCACCCTC AGGACTCTGC TTTCTCTTAC AGAGATGTAA AAAAGAAGCT GAGGCTCGCA 
CTTTGCTCTG CAGACTCTGT GGCCTTCCCA GTGCTAACTC ATTCAACAAG GAATGGATTG 
CCAGACCACA CAGACCCAGA AGACAATGAA ATTGTATGCT TCTTAAAAGT TCAAATAGCT 
GAAGCAATTA ATTTACAAGA TAAGAGCTTA ATGGCTCAAC TTCAAGAAAC AATGCGTTGT 
GTGTGCCGCT TTGACAATAG GACTTGTAGG AAGTTGCTGG CATCCATTGC TGAAGACTAC 
AGAAAAAGAG CTCCGTATAT TGCTTATCTT ACCCGCTGCC GACAAGGACT GCAGACCACA 
CAGGCTCACT TGGAAAGGCT CTTGCAAAGA GTTTTGCGGG ACAAAGAGGT GGCCAATCGG 
TACTTCACCA CTGTCTGTGT GCGGTTACTG CTTGAGAGCA AAGAAAAGAA AATCAGGGAG 
TTCATTCAAG ACTTTCAGAA ACTTACTGCT GCTGATGATA AAACGGCTCA GGTGGAGGAT 
TTTCTACAGT TCCTGTATGG TGTGATGGCT CAGGATGTCA TATGGCAGAA TGCCAGTGAG 
GAGCAGCTGC AGGATGCCCA GCTGGCCATC GAGCGGAGTG TGATGAATCG GATCTTCAAG 
CTTGCTTTCT ACCCTAACCA GGATGGGGAC ATACTTCGTG ACCAGGTTCT GCATGAACAT 
ATCCAGAGAT TATCTAAAGT AGTGACTGCA AACCACAGAG CTCTGCAGAT CCCTGAGGTT 
TATCTCCGTG AGGCACCATG GCCATCTGCA CAGTCAGAAA TCAGGACAAT AAGTGCTTAC 
AAAACGCCCC GCGACAAAGT GCAGTGCATC CTGAGGATGT GCTCCACCAT CATGAACCTC 
TTGAGCCTGG CCAACGAGGA CTCTGTCCCC GGGGCAGATG ACTTTGTTCC TGTGTTGGTG 
TTTGTGTTAA TAAAGGCAAA CCCACCCTGC CTGCTGTCCA CTGTGCAGTA CATCAGCAGC 
TTCTATGCCA GCTGTCTGTC TGGAGAGGAG TCCTACTGGT GGATGCAGTT CACAGCAGCC 
GTGGAGTTTA TTAAAACCAT CGATGACAGA AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A high-resolution anatomical atlas of the transcriptome in the mouse embryo.
PLoS biology9(1)e1000582(2011 Jan)
Diez-Roux G,Banfi S,Sultan M,Geffers L,Anand S,Rozado D,Magen A,Canidio E,Pagani M,Peluso I,Lin-Marq N,Koch M,Bilio M,Cantiello I,Verde R,De Masi C,Bianchi SA,Cicchini J,Perroud E,Mehmeti S,Dagand E,Schrinner S,N?rnberger A,Schmidt K,Metz K,Zwingmann C,Brieske N,Springer C,Hernandez AM,Herzog S,Grabbe F,Sieverding C,Fischer B,Schrader K,Brockmeyer M,Dettmer S,Helbig C,Alunni V,Battaini MA,Mura C,Henrichsen CN,Garcia-Lopez R,Echevarria D,Puelles E,Garcia-Calero E,Kruse S,Uhr M,Kauck C,Feng G,Milyaev N,Ong CK,Kumar L,Lam M,Semple CA,Gyenesei A,Mundlos S,Radelof U,Lehrach H,Sarmientos P,Reymond A,Davidson DR,Doll? P,Antonarakis SE,Yaspo ML,Martinez S,Baldock RA,Eichele G,Ballabio A

Rab22B is expressed in the CNS astroglia lineage and plays a role in epidermal growth factor receptor trafficking in A431 cells.
Journal of cellular physiology221(3)716-28(2009 Dec)
Ng EL,Ng JJ,Liang F,Tang BL

Large-scale gene trapping in C57BL/6N mouse embryonic stem cells.
Genome research18(10)1670-9(2008 Oct)
Hansen GM,Markesich DC,Burnett MB,Zhu Q,Dionne KM,Richter LJ,Finnell RH,Sands AT,Zambrowicz BP,Abuin A

Insulin stimulates phosphatidylinositol 3-phosphate production via the activation of Rab5.
Molecular biology of the cell19(7)2718-28(2008 Jul)
Lodhi IJ,Bridges D,Chiang SH,Zhang Y,Cheng A,Geletka LM,Weisman LS,Saltiel AR

Imaging of Rab5 activity identifies essential regulators for phagosome maturation.
Nature453(7192)241-5(2008 May)
Kitano M,Nakaya M,Nakamura T,Nagata S,Matsuda M

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GAPVD1 cDNA ORF clone, Mus musculus(House mouse)

Gene

Clones

gRNAs

Related articles in PubMed

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