Cyb5b cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cyb5b
Entrez Gene ID	66427
Full Name	cytochrome b5 type B
Synonyms	1810044O22Rik,AU015618,Cyb5m
General protein information	Preferred Names cytochrome b5 type B Names cytochrome b5 type B cytochrome b5 outer mitochondrial membrane isoform
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8\|8 D3

mRNA and Protein(s)

mRNA	Protein	Name
NM_025558.5	NP_079834.2	cytochrome b5 type B precursor

Pathways from BioSystems

Homologies

	CYB5B	XP_001100954.1
Rattus norvegicus (Norway rat)	Cyb5b	NP_085075.1
Homo sapiens (human)	CYB5B	NP_085056.2
Pan troglodytes (chimpanzee)	CYB5B	NP_001229538.1
Schizosaccharomyces pombe (fission yeast)	oca8	NP_587997.1
Arabidopsis thaliana (thale cress)	CB5-D	NP_199692.1
Danio rerio (zebrafish)	cyb5b	NP_998041.1
Caenorhabditis elegans (roundworm)	cytb-5.2	NP_491931.1
Eremothecium gossypii	AGOS_AFL223W	NP_985327.1
Bos taurus (cattle)	CYB5B	NP_001157254.1
Gallus gallus (chicken)	CYB5B	NP_001025752.1
Kluyveromyces lactis	KLLA0F23672g	XP_456135.1
Magnaporthe oryzae (rice blast fungus)	MGG_06510	XP_003717049.1
Xenopus tropicalis (tropical clawed frog)	cyb5b	NP_001011009.2
Mus musculus (house mouse)	Cyb5b	NP_079834.2
Saccharomyces cerevisiae (baker's yeast)	CYB5	NP_014288.3
Neurospora crassa	NCU03910	XP_957549.2

Gene Ontology
Bibliography

Related articles in PubMed

Development of an efficient screening system to identify novel bone metabolism-related genes using the exchangeable gene trap mutagenesis mouse models.
Kurogi S, Sekimoto T, Funamoto T, Ota T, Nakamura S, Nagai T, Nakahara M, Yoshinobu K, Araki K, Araki M, Chosa E
Scientific reports740692(2017 Jan)

The N-reductive system composed of mitochondrial amidoxime reducing component (mARC), cytochrome b5 (CYB5B) and cytochrome b5 reductase (CYB5R) is regulated by fasting and high fat diet in mice.
Jakobs HH, Mikula M, Havemeyer A, Strzalkowska A, Borowa-Chmielak M, Dzwonek A, Gajewska M, Hennig EE, Ostrowski J, Clement B
PloS one9(8)e105371(2014)

Pyrethroid activity-based probes for profiling cytochrome P450 activities associated with insecticide interactions.
Ismail HM, O'Neill PM, Hong DW, Finn RD, Henderson CJ, Wright AT, Cravatt BF, Hemingway J, Paine MJ
Proceedings of the National Academy of Sciences of the United States of America110(49)19766-71(2013 Dec)

Gene-trap mutagenesis using Mol/MSM-1 embryonic stem cells from MSM/Ms mice.
Nakahara M, Tateyama H, Araki M, Nakagata N, Yamamura K, Araki K
Mammalian genome : official journal of the International Mammalian Genome Society24(5-6)228-39(2013 Jun)

Amidoxime reductase system containing cytochrome b5 type B (CYB5B) and MOSC2 is of importance for lipid synthesis in adipocyte mitochondria.
Neve EP, Nordling A, Andersson TB, Hellman U, Diczfalusy U, Johansson I, Ingelman-Sundberg M
The Journal of biological chemistry287(9)6307-17(2012 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The presence of a novel reductive enzyme system of importance for lipid synthesis that is exclusively localized to the outer mitochondrial membrane and is composed of CYB5B, MOSC2, and a third unknown component (a CYB5B reductase).
Title: Amidoxime reductase system containing cytochrome b5 type B (CYB5B) and MOSC2 is of importance for lipid synthesis in adipocyte mitochondria.

microsomal cytochrome b5 can play a major role in the in vivo metabolism of certain drugs and chemicals but in a P450- and substrate-dependent manner
Title: Defining the in Vivo Role for cytochrome b5 in cytochrome P450 function through the conditional hepatic deletion of microsomal cytochrome b5.

the hydroxylation of dihydroceramide: sphinganine C-4 requires complex formation between Des2 and mb5 via their membrane-spanning domains and electron transfer from NADH to the substrate via the reduction of mb5 by b5R
Title: Dihydroceramide:sphinganine C-4-hydroxylation requires Des2 hydroxylase and the membrane form of cytochrome b5.

microsomal cytochrome b5 interacts with neuronal calcium sensor protein visinin-like protein-3 in a Ca2+-dependent manner
Title: Neuronal calcium sensor protein visinin-like protein-3 interacts with microsomal cytochrome b5 in a Ca2+-dependent manner.

The following Cyb5b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cyb5b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu13598	NM_025558.5 Latest version!	Mus musculus cytochrome b5 type B (Cyb5b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cyb5b ( NM_025558.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_079834.2 Mus musculus cytochrome b5 type B (Cyb5b), mRNA.

Price & Availability↑

CloneID	OMu13598
Clone ID Related Accession (Same CDS sequence)	NM_025558.5
Accession Version	NM_025558.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 441bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	cytochrome b5 type B precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC125207.5. On Oct 19, 2007 this sequence version replaced NM_025558.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK168422.1, BC054749.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGGCGACCC CGGAGGCCAG CGGCAGTGGC GAGAAGGTCG AGGGCTCCGA GCCCTCCGTC 
ACCTACTACC GGCTGGAGGA GGTGGCGAAG CGAAACTCGG CGGAAGAGAC GTGGATGGTG 
ATCCACGGGC GAGTCTACGA TATCACCCGC TTCCTCAGCG AGCATCCTGG CGGGGAAGAG 
GTTCTGCTGG AACAAGCGGG TGCTGATGCA ACTGAAAGCT TTGAAGATGT CGGCCACTCC 
CCTGATGCCA GGGAGATGCT AAAGCAGTAC TACATTGGAG ATGTCCATCC GAGTGACCTT 
AAACCTAAAG GCGATGACAA GGATCCTTCC AAGAACAATT CATGCCAAAG TTCCTGGGCG 
TATTGGTTTG TCCCCATCGT GGGCGCTATT CTTATAGGTT TCTTGTATCG TCACTTCTGG 
GCTGACAGCA AATCCTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_079834.2
CDS	52..492
Translation	MATPEASGSGEKVEGSEPSVTYYRLEEVAKRNSAEETWMVIHGRVYDITRFLSEHPGGEEVLLEQAGADATESFEDVGHSPDAREMLKQYYIGDVHPSDLKPKGDDKDPSKNNSCQSSWAYWFVPIVGAILIGFLYRHFWADSKSS

Target ORF information:

RefSeq Version	NM_025558.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus cytochrome b5 type B (Cyb5b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025558.5

ORF Insert Sequence:

ATGGCGACCC CGGAGGCCAG CGGCAGTGGC GAGAAGGTCG AGGGCTCCGA GCCCTCCGTC 
ACCTACTACC GGCTGGAGGA GGTGGCGAAG CGAAACTCGG CGGAAGAGAC GTGGATGGTG 
ATCCACGGGC GAGTCTACGA TATCACCCGC TTCCTCAGCG AGCATCCTGG CGGGGAAGAG 
GTTCTGCTGG AACAAGCGGG TGCTGATGCA ACTGAAAGCT TTGAAGATGT CGGCCACTCC 
CCTGATGCCA GGGAGATGCT AAAGCAGTAC TACATTGGAG ATGTCCATCC GAGTGACCTT 
AAACCTAAAG GCGATGACAA GGATCCTTCC AAGAACAATT CATGCCAAAG TTCCTGGGCG 
TATTGGTTTG TCCCCATCGT GGGCGCTATT CTTATAGGTT TCTTGTATCG TCACTTCTGG 
GCTGACAGCA AATCCTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Development of an efficient screening system to identify novel bone metabolism-related genes using the exchangeable gene trap mutagenesis mouse models.
Scientific reports740692(2017 Jan)
Kurogi S,Sekimoto T,Funamoto T,Ota T,Nakamura S,Nagai T,Nakahara M,Yoshinobu K,Araki K,Araki M,Chosa E

The N-reductive system composed of mitochondrial amidoxime reducing component (mARC), cytochrome b5 (CYB5B) and cytochrome b5 reductase (CYB5R) is regulated by fasting and high fat diet in mice.
PloS one9(8)e105371(2014)
Jakobs HH,Mikula M,Havemeyer A,Strzalkowska A,Borowa-Chmielak M,Dzwonek A,Gajewska M,Hennig EE,Ostrowski J,Clement B

Pyrethroid activity-based probes for profiling cytochrome P450 activities associated with insecticide interactions.
Proceedings of the National Academy of Sciences of the United States of America110(49)19766-71(2013 Dec)
Ismail HM,O'Neill PM,Hong DW,Finn RD,Henderson CJ,Wright AT,Cravatt BF,Hemingway J,Paine MJ

Gene-trap mutagenesis using Mol/MSM-1 embryonic stem cells from MSM/Ms mice.
Mammalian genome : official journal of the International Mammalian Genome Society24(5-6)228-39(2013 Jun)
Nakahara M,Tateyama H,Araki M,Nakagata N,Yamamura K,Araki K

Amidoxime reductase system containing cytochrome b5 type B (CYB5B) and MOSC2 is of importance for lipid synthesis in adipocyte mitochondria.
The Journal of biological chemistry287(9)6307-17(2012 Feb)
Neve EP,Nordling A,Andersson TB,Hellman U,Diczfalusy U,Johansson I,Ingelman-Sundberg M

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

Cyb5b cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報