Smurf2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Smurf2
Entrez Gene ID	66313
Full Name	SMAD specific E3 ubiquitin protein ligase 2
Synonyms	2810411E22Rik,AI558114,AI649275
General protein information	Preferred Names SMAD specific E3 ubiquitin protein ligase 2 Names E3 ubiquitin-protein ligase SMURF2 HECT-type E3 ubiquitin transferase SMURF2 SMAD ubiquitination regulatory factor 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_025481.2	NP_079757.2	E3 ubiquitin-protein ligase SMURF2
XM_017314705.1	XP_017170194.1	E3 ubiquitin-protein ligase SMURF2 isoform X2
NM_025481.3	NP_079757.2	E3 ubiquitin-protein ligase SMURF2 isoform 1
XM_006533936.4	XP_006533999.1	E3 ubiquitin-protein ligase SMURF2 isoform X1
XM_006533936.3	XP_006533999.1	E3 ubiquitin-protein ligase SMURF2 isoform X1
NM_001362894.1	NP_001349823.1	E3 ubiquitin-protein ligase SMURF2 isoform 2

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	lack	NP_523779.1
	SMURF2	XP_001109913.2
Bos taurus (cattle)	SMURF2	XP_002696169.3
Gallus gallus (chicken)	SMURF2	XP_425380.2
Homo sapiens (human)	SMURF2	NP_073576.1
Danio rerio (zebrafish)	smurf2	NP_001107898.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011242	XP_309403.3
Pan troglodytes (chimpanzee)	SMURF2	XP_511577.3
Canis lupus familiaris (dog)	SMURF2	XP_537589.4
Mus musculus (house mouse)	Smurf2	NP_079757.2
Rattus norvegicus (Norway rat)	Smurf2	NP_001100531.1

Gene Ontology
Bibliography

Related articles in PubMed

Smurf2-Mediated Stabilization of DNA Topoisomerase II? Controls Genomic Integrity.
Emanuelli A, Borroni AP, Apel-Sarid L, Shah PA, Ayyathan DM, Koganti P, Levy-Cohen G, Blank M
Cancer research77(16)4217-4227(2017 Aug)

USP4 inhibits SMAD4 monoubiquitination and promotes activin and BMP signaling.
Zhou F, Xie F, Jin K, Zhang Z, Clerici M, Gao R, van Dinther M, Sixma TK, Huang H, Zhang L, Ten Dijke P
The EMBO journal36(11)1623-1639(2017 06)

SMURF2 regulates bone homeostasis by disrupting SMAD3 interaction with vitamin D receptor in osteoblasts.
Xu Z, Greenblatt MB, Yan G, Feng H, Sun J, Lotinun S, Brady N, Baron R, Glimcher LH, Zou W
Nature communications814570(2017 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results identified Smurf2 as an essential regulator of Topo IIalpha, providing novel insights into its control and into the suggested tumor-suppressor functions of Smurf2.
Title: Smurf2-Mediated Stabilization of DNA Topoisomerase II? Controls Genomic Integrity.

Young Smurf2-deficient mice develop milder osteoarthritis in knee articular cartilage compared to WT mice after surgical destabilization of the medial meniscus.
Title: Skeletal Characterization of Smurf2-Deficient Mice and In Vitro Analysis of Smurf2-Deficient Chondrocytes.

TAT fused to WW2/WW3 of Smurf2 could interfere with TGF-beta signaling and reduce ArgI gene expression.
Title: The effects of WW2/WW3 domains of Smurf2 molecule on TGF-? signaling and arginase I gene expression.

Data indicate that TNF receptor associated factor 4 (TRAF4) recruited the E3-ligase SMURF2, to degrade the IL-25R-inhibitory molecule DAZ associated protein 2 (DAZAP2).
Title: TRAF4-SMURF2-mediated DAZAP2 degradation is critical for IL-25 signaling and allergic airway inflammation.

Akt regulates osteoblast differentiation by enhancing the protein stability and transcriptional activity of Runx2 through regulation of degradation of Smurf2.
Title: Akt enhances Runx2 protein stability by regulating Smurf2 function during osteoblast differentiation.

The following Smurf2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Smurf2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu46757	NM_001362894.1 Latest version!	Mus musculus SMAD specific E3 ubiquitin protein ligase 2 (Smurf2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu46757	XM_017314705.1 Latest version!	Mus musculus SMAD specific E3 ubiquitin protein ligase 2 (Smurf2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu19698	NM_025481.3 Latest version!	Mus musculus SMAD specific E3 ubiquitin protein ligase 2 (Smurf2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu46755	XM_006533936.4 Latest version!	Mus musculus SMAD specific E3 ubiquitin protein ligase 2 (Smurf2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OMu19698	NM_025481.2	Mus musculus SMAD specific E3 ubiquitin protein ligase 2 (Smurf2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu46755	XM_006533936.3	Mus musculus SMAD specific E3 ubiquitin protein ligase 2 (Smurf2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Smurf2 ( NM_001362894.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001349823.1 Mus musculus SMAD specific E3 ubiquitin protein ligase 2 (Smurf2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu46757
Clone ID Related Accession (Same CDS sequence)	XM_017314705.1 , NM_001362894.1
Accession Version	NM_001362894.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-23
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase SMURF2 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL593847.36. On May 3, 2018 this sequence version replaced XM_017314705.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660813.107353.1, SRR1660811.196578.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGAGCAGGA CACACTTACA CACTCCTCCA GACCTACCGG AAGGCTATGA ACAAAGGACA 
ACGCAGCAGG GTCAGGTATA TTTCTTACAT ACTCAGACTG GTGTGAGCAC ATGGCATGAT 
CCACGAGTGC CTAGGGATCT TAGCAACATC AATTGTGAAG AGCTCGGTCC TTTGCCTCCT 
GGATGGGAGA TCCGCAATAC CGCAACAGGA AGAGTTTATT TCGTTGACCA TAACAACAGA 
ACAACACAAT TTACAGATCC CCGGCTCTCT GCTAACTTGC ATTTAGTTTT AAATCGTCAG 
AACCAGTTGA AAGACCAACA GCAACAGCAA GTGGTGCCAT TGTGTCCCGA TGACACTGAG 
TGTCTGACAG TGCCAAGATA CAAGCGAGAT TTGGTTCAAA AACTAAAAAT CTTGCGGCAA 
GAACTTTCCC AACAGCAGCC TCAAGCTGGC CACTGCCGTA TTGAGGTGTC TAGGGAAGAG 
ATTTTTGAGG AATCATATAG ACAGGTCATG AAAATGAGGC CAAAAGATCT ATGGAAGCGA 
TTAATGATAA AATTTCGTGG AGAAGAAGGT CTTGACTATG GAGGAGTTGC CAGGGAATGG 
TTGTATCTCC TGTCACATGA AATGTTGAAT CCTTACTATG GTCTCTTCCA GTATTCTAGA 
GATGATATCT ACACATTGCA GATCAATCCT GATTCTGCAG TTAATCCGGA ACATTTATCC 
TATTTCCACT TTGTTGGACG GATAATGGGA ATGGCTGTAT TCCATGGACA TTATATTGAC 
GGTGGCTTCA CATTACCTTT TTATAAACAG TTGCTTGGGA AGTCAATTAC TTTGGATGAC 
ATGGAGCTAG TTGACCCAGA CCTTCATAAT AGTTTAGTGT GGATACTTGA AAACGATATC 
ACAGGTGTTC TGGACCATAC CTTCTGTGTT GAGCATAATG CATATGGTGA AATAATCCAG 
CATGAACTCA AACCAAATGG CAAAAGTATC CCTGTTACTG AAGAAAATAA AAAAGAATAT 
GTCAGGCTTT ATGTCAACTG GAGATTTCTC CGAGGCATCG AAGCTCAGTT CCTGGCGCTG 
CAGAAGGGCT TTAATGAAGT CATTCCCCAG CACCTGCTGA AGACATTTGA TGAGAAGGAG 
CTGGAGCTCA TTATTTGTGG ACTTGGCAAA ATTGATGTGA GTGACTGGAA GGTCAACACC 
CGGTTAAAAC ACTGTACCCC AGACAGCAAT GTCGTCAAGT GGTTCTGGAA AGCGGTGGAG 
TTTTTTGATG AAGAGAGACG AGCACGGTTG CTCCAGTTTG TGACAGGGTC CTCTCGAGTG 
CCTCTGCAGG GCTTCAAAGC ACTACAAGGT GCTGCAGGCC CACGGCTCTT TACCATACAC 
CAGATTGATG CCTGCACGAA CAACTTGCCA AAAGCCCACA CTTGCTTCAA TCGAATAGAC 
ATTCCACCCT ATGAAAGCTA TGAGAAGCTC TATGAAAAGC TGCTAACGGC CATCGAGGAG 
ACATGTGGCT TTGCTGTGGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001349823.1
CDS	1319..2842
Translation	MSRTHLHTPPDLPEGYEQRTTQQGQVYFLHTQTGVSTWHDPRVPRDLSNINCEELGPLPPGWEIRNTATGRVYFVDHNNRTTQFTDPRLSANLHLVLNRQNQLKDQQQQQVVPLCPDDTECLTVPRYKRDLVQKLKILRQELSQQQPQAGHCRIEVSREEIFEESYRQVMKMRPKDLWKRLMIKFRGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSRDDIYTLQINPDSAVNPEHLSYFHFVGRIMGMAVFHGHYIDGGFTLPFYKQLLGKSITLDDMELVDPDLHNSLVWILENDITGVLDHTFCVEHNAYGEIIQHELKPNGKSIPVTEENKKEYVRLYVNWRFLRGIEAQFLALQKGFNEVIPQHLLKTFDEKELELIICGLGKIDVSDWKVNTRLKHCTPDSNVVKWFWKAVEFFDEERRARLLQFVTGSSRVPLQGFKALQGAAGPRLFTIHQIDACTNNLPKAHTCFNRIDIPPYESYEKLYEKLLTAIEETCGFAVE

Target ORF information:

RefSeq Version	NM_001362894.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus SMAD specific E3 ubiquitin protein ligase 2 (Smurf2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001362894.1

ORF Insert Sequence:

ATGAGCAGGA CACACTTACA CACTCCTCCA GACCTACCGG AAGGCTATGA ACAAAGGACA 
ACGCAGCAGG GTCAGGTATA TTTCTTACAT ACTCAGACTG GTGTGAGCAC ATGGCATGAT 
CCACGAGTGC CTAGGGATCT TAGCAACATC AATTGTGAAG AGCTCGGTCC TTTGCCTCCT 
GGATGGGAGA TCCGCAATAC CGCAACAGGA AGAGTTTATT TCGTTGACCA TAACAACAGA 
ACAACACAAT TTACAGATCC CCGGCTCTCT GCTAACTTGC ATTTAGTTTT AAATCGTCAG 
AACCAGTTGA AAGACCAACA GCAACAGCAA GTGGTGCCAT TGTGTCCCGA TGACACTGAG 
TGTCTGACAG TGCCAAGATA CAAGCGAGAT TTGGTTCAAA AACTAAAAAT CTTGCGGCAA 
GAACTTTCCC AACAGCAGCC TCAAGCTGGC CACTGCCGTA TTGAGGTGTC TAGGGAAGAG 
ATTTTTGAGG AATCATATAG ACAGGTCATG AAAATGAGGC CAAAAGATCT ATGGAAGCGA 
TTAATGATAA AATTTCGTGG AGAAGAAGGT CTTGACTATG GAGGAGTTGC CAGGGAATGG 
TTGTATCTCC TGTCACATGA AATGTTGAAT CCTTACTATG GTCTCTTCCA GTATTCTAGA 
GATGATATCT ACACATTGCA GATCAATCCT GATTCTGCAG TTAATCCGGA ACATTTATCC 
TATTTCCACT TTGTTGGACG GATAATGGGA ATGGCTGTAT TCCATGGACA TTATATTGAC 
GGTGGCTTCA CATTACCTTT TTATAAACAG TTGCTTGGGA AGTCAATTAC TTTGGATGAC 
ATGGAGCTAG TTGACCCAGA CCTTCATAAT AGTTTAGTGT GGATACTTGA AAACGATATC 
ACAGGTGTTC TGGACCATAC CTTCTGTGTT GAGCATAATG CATATGGTGA AATAATCCAG 
CATGAACTCA AACCAAATGG CAAAAGTATC CCTGTTACTG AAGAAAATAA AAAAGAATAT 
GTCAGGCTTT ATGTCAACTG GAGATTTCTC CGAGGCATCG AAGCTCAGTT CCTGGCGCTG 
CAGAAGGGCT TTAATGAAGT CATTCCCCAG CACCTGCTGA AGACATTTGA TGAGAAGGAG 
CTGGAGCTCA TTATTTGTGG ACTTGGCAAA ATTGATGTGA GTGACTGGAA GGTCAACACC 
CGGTTAAAAC ACTGTACCCC AGACAGCAAT GTCGTCAAGT GGTTCTGGAA AGCGGTGGAG 
TTTTTTGATG AAGAGAGACG AGCACGGTTG CTCCAGTTTG TGACAGGGTC CTCTCGAGTG 
CCTCTGCAGG GCTTCAAAGC ACTACAAGGT GCTGCAGGCC CACGGCTCTT TACCATACAC 
CAGATTGATG CCTGCACGAA CAACTTGCCA AAAGCCCACA CTTGCTTCAA TCGAATAGAC 
ATTCCACCCT ATGAAAGCTA TGAGAAGCTC TATGAAAAGC TGCTAACGGC CATCGAGGAG 
ACATGTGGCT TTGCTGTGGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smurf2 ( XM_017314705.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017170194.1 Mus musculus SMAD specific E3 ubiquitin protein ligase 2 (Smurf2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu46757
Clone ID Related Accession (Same CDS sequence)	XM_017314705.1 , NM_001362894.1
Accession Version	XM_017314705.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase SMURF2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGGA CACACTTACA CACTCCTCCA GACCTACCGG AAGGCTATGA ACAAAGGACA 
ACGCAGCAGG GTCAGGTATA TTTCTTACAT ACTCAGACTG GTGTGAGCAC ATGGCATGAT 
CCACGAGTGC CTAGGGATCT TAGCAACATC AATTGTGAAG AGCTCGGTCC TTTGCCTCCT 
GGATGGGAGA TCCGCAATAC CGCAACAGGA AGAGTTTATT TCGTTGACCA TAACAACAGA 
ACAACACAAT TTACAGATCC CCGGCTCTCT GCTAACTTGC ATTTAGTTTT AAATCGTCAG 
AACCAGTTGA AAGACCAACA GCAACAGCAA GTGGTGCCAT TGTGTCCCGA TGACACTGAG 
TGTCTGACAG TGCCAAGATA CAAGCGAGAT TTGGTTCAAA AACTAAAAAT CTTGCGGCAA 
GAACTTTCCC AACAGCAGCC TCAAGCTGGC CACTGCCGTA TTGAGGTGTC TAGGGAAGAG 
ATTTTTGAGG AATCATATAG ACAGGTCATG AAAATGAGGC CAAAAGATCT ATGGAAGCGA 
TTAATGATAA AATTTCGTGG AGAAGAAGGT CTTGACTATG GAGGAGTTGC CAGGGAATGG 
TTGTATCTCC TGTCACATGA AATGTTGAAT CCTTACTATG GTCTCTTCCA GTATTCTAGA 
GATGATATCT ACACATTGCA GATCAATCCT GATTCTGCAG TTAATCCGGA ACATTTATCC 
TATTTCCACT TTGTTGGACG GATAATGGGA ATGGCTGTAT TCCATGGACA TTATATTGAC 
GGTGGCTTCA CATTACCTTT TTATAAACAG TTGCTTGGGA AGTCAATTAC TTTGGATGAC 
ATGGAGCTAG TTGACCCAGA CCTTCATAAT AGTTTAGTGT GGATACTTGA AAACGATATC 
ACAGGTGTTC TGGACCATAC CTTCTGTGTT GAGCATAATG CATATGGTGA AATAATCCAG 
CATGAACTCA AACCAAATGG CAAAAGTATC CCTGTTACTG AAGAAAATAA AAAAGAATAT 
GTCAGGCTTT ATGTCAACTG GAGATTTCTC CGAGGCATCG AAGCTCAGTT CCTGGCGCTG 
CAGAAGGGCT TTAATGAAGT CATTCCCCAG CACCTGCTGA AGACATTTGA TGAGAAGGAG 
CTGGAGCTCA TTATTTGTGG ACTTGGCAAA ATTGATGTGA GTGACTGGAA GGTCAACACC 
CGGTTAAAAC ACTGTACCCC AGACAGCAAT GTCGTCAAGT GGTTCTGGAA AGCGGTGGAG 
TTTTTTGATG AAGAGAGACG AGCACGGTTG CTCCAGTTTG TGACAGGGTC CTCTCGAGTG 
CCTCTGCAGG GCTTCAAAGC ACTACAAGGT GCTGCAGGCC CACGGCTCTT TACCATACAC 
CAGATTGATG CCTGCACGAA CAACTTGCCA AAAGCCCACA CTTGCTTCAA TCGAATAGAC 
ATTCCACCCT ATGAAAGCTA TGAGAAGCTC TATGAAAAGC TGCTAACGGC CATCGAGGAG 
ACATGTGGCT TTGCTGTGGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017170194.1
CDS	887..2410
Translation	MSRTHLHTPPDLPEGYEQRTTQQGQVYFLHTQTGVSTWHDPRVPRDLSNINCEELGPLPPGWEIRNTATGRVYFVDHNNRTTQFTDPRLSANLHLVLNRQNQLKDQQQQQVVPLCPDDTECLTVPRYKRDLVQKLKILRQELSQQQPQAGHCRIEVSREEIFEESYRQVMKMRPKDLWKRLMIKFRGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSRDDIYTLQINPDSAVNPEHLSYFHFVGRIMGMAVFHGHYIDGGFTLPFYKQLLGKSITLDDMELVDPDLHNSLVWILENDITGVLDHTFCVEHNAYGEIIQHELKPNGKSIPVTEENKKEYVRLYVNWRFLRGIEAQFLALQKGFNEVIPQHLLKTFDEKELELIICGLGKIDVSDWKVNTRLKHCTPDSNVVKWFWKAVEFFDEERRARLLQFVTGSSRVPLQGFKALQGAAGPRLFTIHQIDACTNNLPKAHTCFNRIDIPPYESYEKLYEKLLTAIEETCGFAVE

Target ORF information:

RefSeq Version	XM_017314705.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus SMAD specific E3 ubiquitin protein ligase 2 (Smurf2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314705.1

ORF Insert Sequence:

ATGAGCAGGA CACACTTACA CACTCCTCCA GACCTACCGG AAGGCTATGA ACAAAGGACA 
ACGCAGCAGG GTCAGGTATA TTTCTTACAT ACTCAGACTG GTGTGAGCAC ATGGCATGAT 
CCACGAGTGC CTAGGGATCT TAGCAACATC AATTGTGAAG AGCTCGGTCC TTTGCCTCCT 
GGATGGGAGA TCCGCAATAC CGCAACAGGA AGAGTTTATT TCGTTGACCA TAACAACAGA 
ACAACACAAT TTACAGATCC CCGGCTCTCT GCTAACTTGC ATTTAGTTTT AAATCGTCAG 
AACCAGTTGA AAGACCAACA GCAACAGCAA GTGGTGCCAT TGTGTCCCGA TGACACTGAG 
TGTCTGACAG TGCCAAGATA CAAGCGAGAT TTGGTTCAAA AACTAAAAAT CTTGCGGCAA 
GAACTTTCCC AACAGCAGCC TCAAGCTGGC CACTGCCGTA TTGAGGTGTC TAGGGAAGAG 
ATTTTTGAGG AATCATATAG ACAGGTCATG AAAATGAGGC CAAAAGATCT ATGGAAGCGA 
TTAATGATAA AATTTCGTGG AGAAGAAGGT CTTGACTATG GAGGAGTTGC CAGGGAATGG 
TTGTATCTCC TGTCACATGA AATGTTGAAT CCTTACTATG GTCTCTTCCA GTATTCTAGA 
GATGATATCT ACACATTGCA GATCAATCCT GATTCTGCAG TTAATCCGGA ACATTTATCC 
TATTTCCACT TTGTTGGACG GATAATGGGA ATGGCTGTAT TCCATGGACA TTATATTGAC 
GGTGGCTTCA CATTACCTTT TTATAAACAG TTGCTTGGGA AGTCAATTAC TTTGGATGAC 
ATGGAGCTAG TTGACCCAGA CCTTCATAAT AGTTTAGTGT GGATACTTGA AAACGATATC 
ACAGGTGTTC TGGACCATAC CTTCTGTGTT GAGCATAATG CATATGGTGA AATAATCCAG 
CATGAACTCA AACCAAATGG CAAAAGTATC CCTGTTACTG AAGAAAATAA AAAAGAATAT 
GTCAGGCTTT ATGTCAACTG GAGATTTCTC CGAGGCATCG AAGCTCAGTT CCTGGCGCTG 
CAGAAGGGCT TTAATGAAGT CATTCCCCAG CACCTGCTGA AGACATTTGA TGAGAAGGAG 
CTGGAGCTCA TTATTTGTGG ACTTGGCAAA ATTGATGTGA GTGACTGGAA GGTCAACACC 
CGGTTAAAAC ACTGTACCCC AGACAGCAAT GTCGTCAAGT GGTTCTGGAA AGCGGTGGAG 
TTTTTTGATG AAGAGAGACG AGCACGGTTG CTCCAGTTTG TGACAGGGTC CTCTCGAGTG 
CCTCTGCAGG GCTTCAAAGC ACTACAAGGT GCTGCAGGCC CACGGCTCTT TACCATACAC 
CAGATTGATG CCTGCACGAA CAACTTGCCA AAAGCCCACA CTTGCTTCAA TCGAATAGAC 
ATTCCACCCT ATGAAAGCTA TGAGAAGCTC TATGAAAAGC TGCTAACGGC CATCGAGGAG 
ACATGTGGCT TTGCTGTGGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smurf2 ( NM_025481.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_079757.2 Mus musculus SMAD specific E3 ubiquitin protein ligase 2 (Smurf2), mRNA.

Price & Availability↑

CloneID	OMu19698
Clone ID Related Accession (Same CDS sequence)	NM_025481.2 , NM_025481.3
Accession Version	NM_025481.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2247bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-27
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase SMURF2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK012060.1, BU058366.1, AK013082.1, BU614468.1, AK036718.1 and BE986450.1. On Jul 31, 2009 this sequence version replaced NM_025481.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660819.150791.1, SRR1660811.163042.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTAACC CCGGAGGCCG GAGGAACGGG CCCGTCAAGC TGCGCCTGAC AGTACTCTGT 
GCAAAAAACT TGGTGAAAAA GGATTTTTTC CGTCTTCCTG ATCCATTTGC TAAGGTGGTA 
GTTGATGGTT CTGGGCAGTG CCATTCTACA GATACTGTGA AGAACACACT TGATCCAAAG 
TGGAATCAGC ATTATGACCT GTATATCGGA AAGTCTGATT CAGTTACGAT CAGCGTATGG 
AATCATAAGA AGATCCATAA GAAGCAAGGT GCTGGATTTC TTGGTTGTGT TCGTCTTCTT 
TCCAATGCCA TCAACCGCCT CAAGGACACT GGTTATCAGA GGTTGGATTT ATGCAAACTG 
GGGCCAAATG ACAATGATAC AGTTCGAGGA CAAATTGTAG TAAGTCTTCA GTCCAGAGAC 
CGAATAGGCA CGGGAGGACA AGTTGTGGAC TGTAGTCGTT TGTTTGACAA TGATTTGCCA 
GATGGTTGGG AAGAAAGGCG GACTGCCTCG GGAAGAATCC AGTATCTAAA CCACATCACA 
AGAACTACAC AGTGGGAACG CCCAACACGA CCGGCGTCAG AATATTCTAG TCCTGGCAGA 
CCCCTCAGCT GCTTTGTGGA TGAGAATACT CCAATTACTG GAACAAATGG TGCAACGTGT 
GGACATTCTT CAGACCCCAG ACTAGCAGAG AGAAGAGTCA GGTCCCAGCG ACATAGAAAT 
TACATGAGCA GGACACACTT ACACACTCCT CCAGACCTAC CGGAAGGCTA TGAACAAAGG 
ACAACGCAGC AGGGTCAGGT ATATTTCTTA CATACTCAGA CTGGTGTGAG CACATGGCAT 
GATCCACGAG TGCCTAGGGA TCTTAGCAAC ATCAATTGTG AAGAGCTCGG TCCTTTGCCT 
CCTGGATGGG AGATCCGCAA TACCGCAACA GGAAGAGTTT ATTTCGTTGA CCATAACAAC 
AGAACAACAC AATTTACAGA TCCCCGGCTC TCTGCTAACT TGCATTTAGT TTTAAATCGT 
CAGAACCAGT TGAAAGACCA ACAGCAACAG CAAGTGGTGC CATTGTGTCC CGATGACACT 
GAGTGTCTGA CAGTGCCAAG ATACAAGCGA GATTTGGTTC AAAAACTAAA AATCTTGCGG 
CAAGAACTTT CCCAACAGCA GCCTCAAGCT GGCCACTGCC GTATTGAGGT GTCTAGGGAA 
GAGATTTTTG AGGAATCATA TAGACAGGTC ATGAAAATGA GGCCAAAAGA TCTATGGAAG 
CGATTAATGA TAAAATTTCG TGGAGAAGAA GGTCTTGACT ATGGAGGAGT TGCCAGGGAA 
TGGTTGTATC TCCTGTCACA TGAAATGTTG AATCCTTACT ATGGTCTCTT CCAGTATTCT 
AGAGATGATA TCTACACATT GCAGATCAAT CCTGATTCTG CAGTTAATCC GGAACATTTA 
TCCTATTTCC ACTTTGTTGG ACGGATAATG GGAATGGCTG TATTCCATGG ACATTATATT 
GACGGTGGCT TCACATTACC TTTTTATAAA CAGTTGCTTG GGAAGTCAAT TACTTTGGAT 
GACATGGAGC TAGTTGACCC AGACCTTCAT AATAGTTTAG TGTGGATACT TGAAAACGAT 
ATCACAGGTG TTCTGGACCA TACCTTCTGT GTTGAGCATA ATGCATATGG TGAAATAATC 
CAGCATGAAC TCAAACCAAA TGGCAAAAGT ATCCCTGTTA CTGAAGAAAA TAAAAAAGAA 
TATGTCAGGC TTTATGTCAA CTGGAGATTT CTCCGAGGCA TCGAAGCTCA GTTCCTGGCG 
CTGCAGAAGG GCTTTAATGA AGTCATTCCC CAGCACCTGC TGAAGACATT TGATGAGAAG 
GAGCTGGAGC TCATTATTTG TGGACTTGGC AAAATTGATG TGAGTGACTG GAAGGTCAAC 
ACCCGGTTAA AACACTGTAC CCCAGACAGC AATGTCGTCA AGTGGTTCTG GAAAGCGGTG 
GAGTTTTTTG ATGAAGAGAG ACGAGCACGG TTGCTCCAGT TTGTGACAGG GTCCTCTCGA 
GTGCCTCTGC AGGGCTTCAA AGCACTACAA GGTGCTGCAG GCCCACGGCT CTTTACCATA 
CACCAGATTG ATGCCTGCAC GAACAACTTG CCAAAAGCCC ACACTTGCTT CAATCGAATA 
GACATTCCAC CCTATGAAAG CTATGAGAAG CTCTATGAAA AGCTGCTAAC GGCCATCGAG 
GAGACATGTG GCTTTGCTGT GGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_079757.2
CDS	484..2730
Translation	MSNPGGRRNGPVKLRLTVLCAKNLVKKDFFRLPDPFAKVVVDGSGQCHSTDTVKNTLDPKWNQHYDLYIGKSDSVTISVWNHKKIHKKQGAGFLGCVRLLSNAINRLKDTGYQRLDLCKLGPNDNDTVRGQIVVSLQSRDRIGTGGQVVDCSRLFDNDLPDGWEERRTASGRIQYLNHITRTTQWERPTRPASEYSSPGRPLSCFVDENTPITGTNGATCGHSSDPRLAERRVRSQRHRNYMSRTHLHTPPDLPEGYEQRTTQQGQVYFLHTQTGVSTWHDPRVPRDLSNINCEELGPLPPGWEIRNTATGRVYFVDHNNRTTQFTDPRLSANLHLVLNRQNQLKDQQQQQVVPLCPDDTECLTVPRYKRDLVQKLKILRQELSQQQPQAGHCRIEVSREEIFEESYRQVMKMRPKDLWKRLMIKFRGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSRDDIYTLQINPDSAVNPEHLSYFHFVGRIMGMAVFHGHYIDGGFTLPFYKQLLGKSITLDDMELVDPDLHNSLVWILENDITGVLDHTFCVEHNAYGEIIQHELKPNGKSIPVTEENKKEYVRLYVNWRFLRGIEAQFLALQKGFNEVIPQHLLKTFDEKELELIICGLGKIDVSDWKVNTRLKHCTPDSNVVKWFWKAVEFFDEERRARLLQFVTGSSRVPLQGFKALQGAAGPRLFTIHQIDACTNNLPKAHTCFNRIDIPPYESYEKLYEKLLTAIEETCGFAVE

Target ORF information:

RefSeq Version	NM_025481.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus SMAD specific E3 ubiquitin protein ligase 2 (Smurf2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025481.2

ORF Insert Sequence:

ATGTCTAACC CCGGAGGCCG GAGGAACGGG CCCGTCAAGC TGCGCCTGAC AGTACTCTGT 
GCAAAAAACT TGGTGAAAAA GGATTTTTTC CGTCTTCCTG ATCCATTTGC TAAGGTGGTA 
GTTGATGGTT CTGGGCAGTG CCATTCTACA GATACTGTGA AGAACACACT TGATCCAAAG 
TGGAATCAGC ATTATGACCT GTATATCGGA AAGTCTGATT CAGTTACGAT CAGCGTATGG 
AATCATAAGA AGATCCATAA GAAGCAAGGT GCTGGATTTC TTGGTTGTGT TCGTCTTCTT 
TCCAATGCCA TCAACCGCCT CAAGGACACT GGTTATCAGA GGTTGGATTT ATGCAAACTG 
GGGCCAAATG ACAATGATAC AGTTCGAGGA CAAATTGTAG TAAGTCTTCA GTCCAGAGAC 
CGAATAGGCA CGGGAGGACA AGTTGTGGAC TGTAGTCGTT TGTTTGACAA TGATTTGCCA 
GATGGTTGGG AAGAAAGGCG GACTGCCTCG GGAAGAATCC AGTATCTAAA CCACATCACA 
AGAACTACAC AGTGGGAACG CCCAACACGA CCGGCGTCAG AATATTCTAG TCCTGGCAGA 
CCCCTCAGCT GCTTTGTGGA TGAGAATACT CCAATTACTG GAACAAATGG TGCAACGTGT 
GGACATTCTT CAGACCCCAG ACTAGCAGAG AGAAGAGTCA GGTCCCAGCG ACATAGAAAT 
TACATGAGCA GGACACACTT ACACACTCCT CCAGACCTAC CGGAAGGCTA TGAACAAAGG 
ACAACGCAGC AGGGTCAGGT ATATTTCTTA CATACTCAGA CTGGTGTGAG CACATGGCAT 
GATCCACGAG TGCCTAGGGA TCTTAGCAAC ATCAATTGTG AAGAGCTCGG TCCTTTGCCT 
CCTGGATGGG AGATCCGCAA TACCGCAACA GGAAGAGTTT ATTTCGTTGA CCATAACAAC 
AGAACAACAC AATTTACAGA TCCCCGGCTC TCTGCTAACT TGCATTTAGT TTTAAATCGT 
CAGAACCAGT TGAAAGACCA ACAGCAACAG CAAGTGGTGC CATTGTGTCC CGATGACACT 
GAGTGTCTGA CAGTGCCAAG ATACAAGCGA GATTTGGTTC AAAAACTAAA AATCTTGCGG 
CAAGAACTTT CCCAACAGCA GCCTCAAGCT GGCCACTGCC GTATTGAGGT GTCTAGGGAA 
GAGATTTTTG AGGAATCATA TAGACAGGTC ATGAAAATGA GGCCAAAAGA TCTATGGAAG 
CGATTAATGA TAAAATTTCG TGGAGAAGAA GGTCTTGACT ATGGAGGAGT TGCCAGGGAA 
TGGTTGTATC TCCTGTCACA TGAAATGTTG AATCCTTACT ATGGTCTCTT CCAGTATTCT 
AGAGATGATA TCTACACATT GCAGATCAAT CCTGATTCTG CAGTTAATCC GGAACATTTA 
TCCTATTTCC ACTTTGTTGG ACGGATAATG GGAATGGCTG TATTCCATGG ACATTATATT 
GACGGTGGCT TCACATTACC TTTTTATAAA CAGTTGCTTG GGAAGTCAAT TACTTTGGAT 
GACATGGAGC TAGTTGACCC AGACCTTCAT AATAGTTTAG TGTGGATACT TGAAAACGAT 
ATCACAGGTG TTCTGGACCA TACCTTCTGT GTTGAGCATA ATGCATATGG TGAAATAATC 
CAGCATGAAC TCAAACCAAA TGGCAAAAGT ATCCCTGTTA CTGAAGAAAA TAAAAAAGAA 
TATGTCAGGC TTTATGTCAA CTGGAGATTT CTCCGAGGCA TCGAAGCTCA GTTCCTGGCG 
CTGCAGAAGG GCTTTAATGA AGTCATTCCC CAGCACCTGC TGAAGACATT TGATGAGAAG 
GAGCTGGAGC TCATTATTTG TGGACTTGGC AAAATTGATG TGAGTGACTG GAAGGTCAAC 
ACCCGGTTAA AACACTGTAC CCCAGACAGC AATGTCGTCA AGTGGTTCTG GAAAGCGGTG 
GAGTTTTTTG ATGAAGAGAG ACGAGCACGG TTGCTCCAGT TTGTGACAGG GTCCTCTCGA 
GTGCCTCTGC AGGGCTTCAA AGCACTACAA GGTGCTGCAG GCCCACGGCT CTTTACCATA 
CACCAGATTG ATGCCTGCAC GAACAACTTG CCAAAAGCCC ACACTTGCTT CAATCGAATA 
GACATTCCAC CCTATGAAAG CTATGAGAAG CTCTATGAAA AGCTGCTAAC GGCCATCGAG 
GAGACATGTG GCTTTGCTGT GGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smurf2 ( NM_025481.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_079757.2 Mus musculus SMAD specific E3 ubiquitin protein ligase 2 (Smurf2), transcript variant 1, mRNA.

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CloneID	OMu19698
Clone ID Related Accession (Same CDS sequence)	NM_025481.2 , NM_025481.3
Accession Version	NM_025481.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2247bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-22
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase SMURF2 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK012060.1, BU058366.1, AK013082.1, BU614468.1, AK036718.1, BE986450.1 and AL593847.36. On May 3, 2018 this sequence version replaced NM_025481.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660819.150791.1, SRR1660811.163042.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTAACC CCGGAGGCCG GAGGAACGGG CCCGTCAAGC TGCGCCTGAC AGTACTCTGT 
GCAAAAAACT TGGTGAAAAA GGATTTTTTC CGTCTTCCTG ATCCATTTGC TAAGGTGGTA 
GTTGATGGTT CTGGGCAGTG CCATTCTACA GATACTGTGA AGAACACACT TGATCCAAAG 
TGGAATCAGC ATTATGACCT GTATATCGGA AAGTCTGATT CAGTTACGAT CAGCGTATGG 
AATCATAAGA AGATCCATAA GAAGCAAGGT GCTGGATTTC TTGGTTGTGT TCGTCTTCTT 
TCCAATGCCA TCAACCGCCT CAAGGACACT GGTTATCAGA GGTTGGATTT ATGCAAACTG 
GGGCCAAATG ACAATGATAC AGTTCGAGGA CAAATTGTAG TAAGTCTTCA GTCCAGAGAC 
CGAATAGGCA CGGGAGGACA AGTTGTGGAC TGTAGTCGTT TGTTTGACAA TGATTTGCCA 
GATGGTTGGG AAGAAAGGCG GACTGCCTCG GGAAGAATCC AGTATCTAAA CCACATCACA 
AGAACTACAC AGTGGGAACG CCCAACACGA CCGGCGTCAG AATATTCTAG TCCTGGCAGA 
CCCCTCAGCT GCTTTGTGGA TGAGAATACT CCAATTACTG GAACAAATGG TGCAACGTGT 
GGACATTCTT CAGACCCCAG ACTAGCAGAG AGAAGAGTCA GGTCCCAGCG ACATAGAAAT 
TACATGAGCA GGACACACTT ACACACTCCT CCAGACCTAC CGGAAGGCTA TGAACAAAGG 
ACAACGCAGC AGGGTCAGGT ATATTTCTTA CATACTCAGA CTGGTGTGAG CACATGGCAT 
GATCCACGAG TGCCTAGGGA TCTTAGCAAC ATCAATTGTG AAGAGCTCGG TCCTTTGCCT 
CCTGGATGGG AGATCCGCAA TACCGCAACA GGAAGAGTTT ATTTCGTTGA CCATAACAAC 
AGAACAACAC AATTTACAGA TCCCCGGCTC TCTGCTAACT TGCATTTAGT TTTAAATCGT 
CAGAACCAGT TGAAAGACCA ACAGCAACAG CAAGTGGTGC CATTGTGTCC CGATGACACT 
GAGTGTCTGA CAGTGCCAAG ATACAAGCGA GATTTGGTTC AAAAACTAAA AATCTTGCGG 
CAAGAACTTT CCCAACAGCA GCCTCAAGCT GGCCACTGCC GTATTGAGGT GTCTAGGGAA 
GAGATTTTTG AGGAATCATA TAGACAGGTC ATGAAAATGA GGCCAAAAGA TCTATGGAAG 
CGATTAATGA TAAAATTTCG TGGAGAAGAA GGTCTTGACT ATGGAGGAGT TGCCAGGGAA 
TGGTTGTATC TCCTGTCACA TGAAATGTTG AATCCTTACT ATGGTCTCTT CCAGTATTCT 
AGAGATGATA TCTACACATT GCAGATCAAT CCTGATTCTG CAGTTAATCC GGAACATTTA 
TCCTATTTCC ACTTTGTTGG ACGGATAATG GGAATGGCTG TATTCCATGG ACATTATATT 
GACGGTGGCT TCACATTACC TTTTTATAAA CAGTTGCTTG GGAAGTCAAT TACTTTGGAT 
GACATGGAGC TAGTTGACCC AGACCTTCAT AATAGTTTAG TGTGGATACT TGAAAACGAT 
ATCACAGGTG TTCTGGACCA TACCTTCTGT GTTGAGCATA ATGCATATGG TGAAATAATC 
CAGCATGAAC TCAAACCAAA TGGCAAAAGT ATCCCTGTTA CTGAAGAAAA TAAAAAAGAA 
TATGTCAGGC TTTATGTCAA CTGGAGATTT CTCCGAGGCA TCGAAGCTCA GTTCCTGGCG 
CTGCAGAAGG GCTTTAATGA AGTCATTCCC CAGCACCTGC TGAAGACATT TGATGAGAAG 
GAGCTGGAGC TCATTATTTG TGGACTTGGC AAAATTGATG TGAGTGACTG GAAGGTCAAC 
ACCCGGTTAA AACACTGTAC CCCAGACAGC AATGTCGTCA AGTGGTTCTG GAAAGCGGTG 
GAGTTTTTTG ATGAAGAGAG ACGAGCACGG TTGCTCCAGT TTGTGACAGG GTCCTCTCGA 
GTGCCTCTGC AGGGCTTCAA AGCACTACAA GGTGCTGCAG GCCCACGGCT CTTTACCATA 
CACCAGATTG ATGCCTGCAC GAACAACTTG CCAAAAGCCC ACACTTGCTT CAATCGAATA 
GACATTCCAC CCTATGAAAG CTATGAGAAG CTCTATGAAA AGCTGCTAAC GGCCATCGAG 
GAGACATGTG GCTTTGCTGT GGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_079757.2
CDS	484..2730
Translation	MSNPGGRRNGPVKLRLTVLCAKNLVKKDFFRLPDPFAKVVVDGSGQCHSTDTVKNTLDPKWNQHYDLYIGKSDSVTISVWNHKKIHKKQGAGFLGCVRLLSNAINRLKDTGYQRLDLCKLGPNDNDTVRGQIVVSLQSRDRIGTGGQVVDCSRLFDNDLPDGWEERRTASGRIQYLNHITRTTQWERPTRPASEYSSPGRPLSCFVDENTPITGTNGATCGHSSDPRLAERRVRSQRHRNYMSRTHLHTPPDLPEGYEQRTTQQGQVYFLHTQTGVSTWHDPRVPRDLSNINCEELGPLPPGWEIRNTATGRVYFVDHNNRTTQFTDPRLSANLHLVLNRQNQLKDQQQQQVVPLCPDDTECLTVPRYKRDLVQKLKILRQELSQQQPQAGHCRIEVSREEIFEESYRQVMKMRPKDLWKRLMIKFRGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSRDDIYTLQINPDSAVNPEHLSYFHFVGRIMGMAVFHGHYIDGGFTLPFYKQLLGKSITLDDMELVDPDLHNSLVWILENDITGVLDHTFCVEHNAYGEIIQHELKPNGKSIPVTEENKKEYVRLYVNWRFLRGIEAQFLALQKGFNEVIPQHLLKTFDEKELELIICGLGKIDVSDWKVNTRLKHCTPDSNVVKWFWKAVEFFDEERRARLLQFVTGSSRVPLQGFKALQGAAGPRLFTIHQIDACTNNLPKAHTCFNRIDIPPYESYEKLYEKLLTAIEETCGFAVE

Target ORF information:

RefSeq Version	NM_025481.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus SMAD specific E3 ubiquitin protein ligase 2 (Smurf2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025481.3

ORF Insert Sequence:

ATGTCTAACC CCGGAGGCCG GAGGAACGGG CCCGTCAAGC TGCGCCTGAC AGTACTCTGT 
GCAAAAAACT TGGTGAAAAA GGATTTTTTC CGTCTTCCTG ATCCATTTGC TAAGGTGGTA 
GTTGATGGTT CTGGGCAGTG CCATTCTACA GATACTGTGA AGAACACACT TGATCCAAAG 
TGGAATCAGC ATTATGACCT GTATATCGGA AAGTCTGATT CAGTTACGAT CAGCGTATGG 
AATCATAAGA AGATCCATAA GAAGCAAGGT GCTGGATTTC TTGGTTGTGT TCGTCTTCTT 
TCCAATGCCA TCAACCGCCT CAAGGACACT GGTTATCAGA GGTTGGATTT ATGCAAACTG 
GGGCCAAATG ACAATGATAC AGTTCGAGGA CAAATTGTAG TAAGTCTTCA GTCCAGAGAC 
CGAATAGGCA CGGGAGGACA AGTTGTGGAC TGTAGTCGTT TGTTTGACAA TGATTTGCCA 
GATGGTTGGG AAGAAAGGCG GACTGCCTCG GGAAGAATCC AGTATCTAAA CCACATCACA 
AGAACTACAC AGTGGGAACG CCCAACACGA CCGGCGTCAG AATATTCTAG TCCTGGCAGA 
CCCCTCAGCT GCTTTGTGGA TGAGAATACT CCAATTACTG GAACAAATGG TGCAACGTGT 
GGACATTCTT CAGACCCCAG ACTAGCAGAG AGAAGAGTCA GGTCCCAGCG ACATAGAAAT 
TACATGAGCA GGACACACTT ACACACTCCT CCAGACCTAC CGGAAGGCTA TGAACAAAGG 
ACAACGCAGC AGGGTCAGGT ATATTTCTTA CATACTCAGA CTGGTGTGAG CACATGGCAT 
GATCCACGAG TGCCTAGGGA TCTTAGCAAC ATCAATTGTG AAGAGCTCGG TCCTTTGCCT 
CCTGGATGGG AGATCCGCAA TACCGCAACA GGAAGAGTTT ATTTCGTTGA CCATAACAAC 
AGAACAACAC AATTTACAGA TCCCCGGCTC TCTGCTAACT TGCATTTAGT TTTAAATCGT 
CAGAACCAGT TGAAAGACCA ACAGCAACAG CAAGTGGTGC CATTGTGTCC CGATGACACT 
GAGTGTCTGA CAGTGCCAAG ATACAAGCGA GATTTGGTTC AAAAACTAAA AATCTTGCGG 
CAAGAACTTT CCCAACAGCA GCCTCAAGCT GGCCACTGCC GTATTGAGGT GTCTAGGGAA 
GAGATTTTTG AGGAATCATA TAGACAGGTC ATGAAAATGA GGCCAAAAGA TCTATGGAAG 
CGATTAATGA TAAAATTTCG TGGAGAAGAA GGTCTTGACT ATGGAGGAGT TGCCAGGGAA 
TGGTTGTATC TCCTGTCACA TGAAATGTTG AATCCTTACT ATGGTCTCTT CCAGTATTCT 
AGAGATGATA TCTACACATT GCAGATCAAT CCTGATTCTG CAGTTAATCC GGAACATTTA 
TCCTATTTCC ACTTTGTTGG ACGGATAATG GGAATGGCTG TATTCCATGG ACATTATATT 
GACGGTGGCT TCACATTACC TTTTTATAAA CAGTTGCTTG GGAAGTCAAT TACTTTGGAT 
GACATGGAGC TAGTTGACCC AGACCTTCAT AATAGTTTAG TGTGGATACT TGAAAACGAT 
ATCACAGGTG TTCTGGACCA TACCTTCTGT GTTGAGCATA ATGCATATGG TGAAATAATC 
CAGCATGAAC TCAAACCAAA TGGCAAAAGT ATCCCTGTTA CTGAAGAAAA TAAAAAAGAA 
TATGTCAGGC TTTATGTCAA CTGGAGATTT CTCCGAGGCA TCGAAGCTCA GTTCCTGGCG 
CTGCAGAAGG GCTTTAATGA AGTCATTCCC CAGCACCTGC TGAAGACATT TGATGAGAAG 
GAGCTGGAGC TCATTATTTG TGGACTTGGC AAAATTGATG TGAGTGACTG GAAGGTCAAC 
ACCCGGTTAA AACACTGTAC CCCAGACAGC AATGTCGTCA AGTGGTTCTG GAAAGCGGTG 
GAGTTTTTTG ATGAAGAGAG ACGAGCACGG TTGCTCCAGT TTGTGACAGG GTCCTCTCGA 
GTGCCTCTGC AGGGCTTCAA AGCACTACAA GGTGCTGCAG GCCCACGGCT CTTTACCATA 
CACCAGATTG ATGCCTGCAC GAACAACTTG CCAAAAGCCC ACACTTGCTT CAATCGAATA 
GACATTCCAC CCTATGAAAG CTATGAGAAG CTCTATGAAA AGCTGCTAAC GGCCATCGAG 
GAGACATGTG GCTTTGCTGT GGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smurf2 ( XM_006533936.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006533999.1 Mus musculus SMAD specific E3 ubiquitin protein ligase 2 (Smurf2), transcript variant X1, mRNA.

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CloneID	OMu46755
Clone ID Related Accession (Same CDS sequence)	XM_006533936.4 , XM_006533936.3
Accession Version	XM_006533936.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase SMURF2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006533936.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAACC CCGGAGGCCG GAGGAACGGG CCCGTCAAGC TGCGCCTGAC AGGTCTTCCT 
GATCCATTTG CTAAGGTGGT AGTTGATGGT TCTGGGCAGT GCCATTCTAC AGATACTGTG 
AAGAACACAC TTGATCCAAA GTGGAATCAG CATTATGACC TGTATATCGG AAAGTCTGAT 
TCAGTTACGA TCAGCGTATG GAATCATAAG AAGATCCATA AGAAGCAAGG TGCTGGATTT 
CTTGGTTGTG TTCGTCTTCT TTCCAATGCC ATCAACCGCC TCAAGGACAC TGGTTATCAG 
AGGTTGGATT TATGCAAACT GGGGCCAAAT GACAATGATA CAGTTCGAGG ACAAATTGTA 
GTAAGTCTTC AGTCCAGAGA CCGAATAGGC ACGGGAGGAC AAGTTGTGGA CTGTAGTCGT 
TTGTTTGACA ATGATTTGCC AGATGGTTGG GAAGAAAGGC GGACTGCCTC GGGAAGAATC 
CAGTATCTAA ACCACATCAC AAGAACTACA CAGTGGGAAC GCCCAACACG ACCGGCGTCA 
GAATATTCTA GTCCTGGCAG ACCCCTCAGC TGCTTTGTGG ATGAGAATAC TCCAATTACT 
GGAACAAATG GTGCAACGTG TGGACATTCT TCAGACCCCA GACTAGCAGA GAGAAGAGTC 
AGGTCCCAGC GACATAGAAA TTACATGAGC AGGACACACT TACACACTCC TCCAGACCTA 
CCGGAAGGCT ATGAACAAAG GACAACGCAG CAGGGTCAGG TATATTTCTT ACATACTCAG 
ACTGGTGTGA GCACATGGCA TGATCCACGA GTGCCTAGGG ATCTTAGCAA CATCAATTGT 
GAAGAGCTCG GTCCTTTGCC TCCTGGATGG GAGATCCGCA ATACCGCAAC AGGAAGAGTT 
TATTTCGTTG ACCATAACAA CAGAACAACA CAATTTACAG ATCCCCGGCT CTCTGCTAAC 
TTGCATTTAG TTTTAAATCG TCAGAACCAG TTGAAAGACC AACAGCAACA GCAAGTGGTG 
CCATTGTGTC CCGATGACAC TGAGTGTCTG ACAGTGCCAA GATACAAGCG AGATTTGGTT 
CAAAAACTAA AAATCTTGCG GCAAGAACTT TCCCAACAGC AGCCTCAAGC TGGCCACTGC 
CGTATTGAGG TGTCTAGGGA AGAGATTTTT GAGGAATCAT ATAGACAGGT CATGAAAATG 
AGGCCAAAAG ATCTATGGAA GCGATTAATG ATAAAATTTC GTGGAGAAGA AGGTCTTGAC 
TATGGAGGAG TTGCCAGGGA ATGGTTGTAT CTCCTGTCAC ATGAAATGTT GAATCCTTAC 
TATGGTCTCT TCCAGTATTC TAGAGATGAT ATCTACACAT TGCAGATCAA TCCTGATTCT 
GCAGTTAATC CGGAACATTT ATCCTATTTC CACTTTGTTG GACGGATAAT GGGAATGGCT 
GTATTCCATG GACATTATAT TGACGGTGGC TTCACATTAC CTTTTTATAA ACAGTTGCTT 
GGGAAGTCAA TTACTTTGGA TGACATGGAG CTAGTTGACC CAGACCTTCA TAATAGTTTA 
GTGTGGATAC TTGAAAACGA TATCACAGGT GTTCTGGACC ATACCTTCTG TGTTGAGCAT 
AATGCATATG GTGAAATAAT CCAGCATGAA CTCAAACCAA ATGGCAAAAG TATCCCTGTT 
ACTGAAGAAA ATAAAAAAGA ATATGTCAGG CTTTATGTCA ACTGGAGATT TCTCCGAGGC 
ATCGAAGCTC AGTTCCTGGC GCTGCAGAAG GGCTTTAATG AAGTCATTCC CCAGCACCTG 
CTGAAGACAT TTGATGAGAA GGAGCTGGAG CTCATTATTT GTGGACTTGG CAAAATTGAT 
GTGAGTGACT GGAAGGTCAA CACCCGGTTA AAACACTGTA CCCCAGACAG CAATGTCGTC 
AAGTGGTTCT GGAAAGCGGT GGAGTTTTTT GATGAAGAGA GACGAGCACG GTTGCTCCAG 
TTTGTGACAG GGTCCTCTCG AGTGCCTCTG CAGGGCTTCA AAGCACTACA AGGTGCTGCA 
GGCCCACGGC TCTTTACCAT ACACCAGATT GATGCCTGCA CGAACAACTT GCCAAAAGCC 
CACACTTGCT TCAATCGAAT AGACATTCCA CCCTATGAAA GCTATGAGAA GCTCTATGAA 
AAGCTGCTAA CGGCCATCGA GGAGACATGT GGCTTTGCTG TGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006533999.1
CDS	478..2685
Translation	MSNPGGRRNGPVKLRLTGLPDPFAKVVVDGSGQCHSTDTVKNTLDPKWNQHYDLYIGKSDSVTISVWNHKKIHKKQGAGFLGCVRLLSNAINRLKDTGYQRLDLCKLGPNDNDTVRGQIVVSLQSRDRIGTGGQVVDCSRLFDNDLPDGWEERRTASGRIQYLNHITRTTQWERPTRPASEYSSPGRPLSCFVDENTPITGTNGATCGHSSDPRLAERRVRSQRHRNYMSRTHLHTPPDLPEGYEQRTTQQGQVYFLHTQTGVSTWHDPRVPRDLSNINCEELGPLPPGWEIRNTATGRVYFVDHNNRTTQFTDPRLSANLHLVLNRQNQLKDQQQQQVVPLCPDDTECLTVPRYKRDLVQKLKILRQELSQQQPQAGHCRIEVSREEIFEESYRQVMKMRPKDLWKRLMIKFRGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSRDDIYTLQINPDSAVNPEHLSYFHFVGRIMGMAVFHGHYIDGGFTLPFYKQLLGKSITLDDMELVDPDLHNSLVWILENDITGVLDHTFCVEHNAYGEIIQHELKPNGKSIPVTEENKKEYVRLYVNWRFLRGIEAQFLALQKGFNEVIPQHLLKTFDEKELELIICGLGKIDVSDWKVNTRLKHCTPDSNVVKWFWKAVEFFDEERRARLLQFVTGSSRVPLQGFKALQGAAGPRLFTIHQIDACTNNLPKAHTCFNRIDIPPYESYEKLYEKLLTAIEETCGFAVE

Target ORF information:

RefSeq Version	XM_006533936.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus SMAD specific E3 ubiquitin protein ligase 2 (Smurf2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006533936.3

ORF Insert Sequence:

ATGTCTAACC CCGGAGGCCG GAGGAACGGG CCCGTCAAGC TGCGCCTGAC AGGTCTTCCT 
GATCCATTTG CTAAGGTGGT AGTTGATGGT TCTGGGCAGT GCCATTCTAC AGATACTGTG 
AAGAACACAC TTGATCCAAA GTGGAATCAG CATTATGACC TGTATATCGG AAAGTCTGAT 
TCAGTTACGA TCAGCGTATG GAATCATAAG AAGATCCATA AGAAGCAAGG TGCTGGATTT 
CTTGGTTGTG TTCGTCTTCT TTCCAATGCC ATCAACCGCC TCAAGGACAC TGGTTATCAG 
AGGTTGGATT TATGCAAACT GGGGCCAAAT GACAATGATA CAGTTCGAGG ACAAATTGTA 
GTAAGTCTTC AGTCCAGAGA CCGAATAGGC ACGGGAGGAC AAGTTGTGGA CTGTAGTCGT 
TTGTTTGACA ATGATTTGCC AGATGGTTGG GAAGAAAGGC GGACTGCCTC GGGAAGAATC 
CAGTATCTAA ACCACATCAC AAGAACTACA CAGTGGGAAC GCCCAACACG ACCGGCGTCA 
GAATATTCTA GTCCTGGCAG ACCCCTCAGC TGCTTTGTGG ATGAGAATAC TCCAATTACT 
GGAACAAATG GTGCAACGTG TGGACATTCT TCAGACCCCA GACTAGCAGA GAGAAGAGTC 
AGGTCCCAGC GACATAGAAA TTACATGAGC AGGACACACT TACACACTCC TCCAGACCTA 
CCGGAAGGCT ATGAACAAAG GACAACGCAG CAGGGTCAGG TATATTTCTT ACATACTCAG 
ACTGGTGTGA GCACATGGCA TGATCCACGA GTGCCTAGGG ATCTTAGCAA CATCAATTGT 
GAAGAGCTCG GTCCTTTGCC TCCTGGATGG GAGATCCGCA ATACCGCAAC AGGAAGAGTT 
TATTTCGTTG ACCATAACAA CAGAACAACA CAATTTACAG ATCCCCGGCT CTCTGCTAAC 
TTGCATTTAG TTTTAAATCG TCAGAACCAG TTGAAAGACC AACAGCAACA GCAAGTGGTG 
CCATTGTGTC CCGATGACAC TGAGTGTCTG ACAGTGCCAA GATACAAGCG AGATTTGGTT 
CAAAAACTAA AAATCTTGCG GCAAGAACTT TCCCAACAGC AGCCTCAAGC TGGCCACTGC 
CGTATTGAGG TGTCTAGGGA AGAGATTTTT GAGGAATCAT ATAGACAGGT CATGAAAATG 
AGGCCAAAAG ATCTATGGAA GCGATTAATG ATAAAATTTC GTGGAGAAGA AGGTCTTGAC 
TATGGAGGAG TTGCCAGGGA ATGGTTGTAT CTCCTGTCAC ATGAAATGTT GAATCCTTAC 
TATGGTCTCT TCCAGTATTC TAGAGATGAT ATCTACACAT TGCAGATCAA TCCTGATTCT 
GCAGTTAATC CGGAACATTT ATCCTATTTC CACTTTGTTG GACGGATAAT GGGAATGGCT 
GTATTCCATG GACATTATAT TGACGGTGGC TTCACATTAC CTTTTTATAA ACAGTTGCTT 
GGGAAGTCAA TTACTTTGGA TGACATGGAG CTAGTTGACC CAGACCTTCA TAATAGTTTA 
GTGTGGATAC TTGAAAACGA TATCACAGGT GTTCTGGACC ATACCTTCTG TGTTGAGCAT 
AATGCATATG GTGAAATAAT CCAGCATGAA CTCAAACCAA ATGGCAAAAG TATCCCTGTT 
ACTGAAGAAA ATAAAAAAGA ATATGTCAGG CTTTATGTCA ACTGGAGATT TCTCCGAGGC 
ATCGAAGCTC AGTTCCTGGC GCTGCAGAAG GGCTTTAATG AAGTCATTCC CCAGCACCTG 
CTGAAGACAT TTGATGAGAA GGAGCTGGAG CTCATTATTT GTGGACTTGG CAAAATTGAT 
GTGAGTGACT GGAAGGTCAA CACCCGGTTA AAACACTGTA CCCCAGACAG CAATGTCGTC 
AAGTGGTTCT GGAAAGCGGT GGAGTTTTTT GATGAAGAGA GACGAGCACG GTTGCTCCAG 
TTTGTGACAG GGTCCTCTCG AGTGCCTCTG CAGGGCTTCA AAGCACTACA AGGTGCTGCA 
GGCCCACGGC TCTTTACCAT ACACCAGATT GATGCCTGCA CGAACAACTT GCCAAAAGCC 
CACACTTGCT TCAATCGAAT AGACATTCCA CCCTATGAAA GCTATGAGAA GCTCTATGAA 
AAGCTGCTAA CGGCCATCGA GGAGACATGT GGCTTTGCTG TGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smurf2 ( XM_006533936.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006533999.1 Mus musculus SMAD specific E3 ubiquitin protein ligase 2 (Smurf2), transcript variant X1, mRNA.

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CloneID	OMu46755
Clone ID Related Accession (Same CDS sequence)	XM_006533936.4 , XM_006533936.3
Accession Version	XM_006533936.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase SMURF2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006533936.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAACC CCGGAGGCCG GAGGAACGGG CCCGTCAAGC TGCGCCTGAC AGGTCTTCCT 
GATCCATTTG CTAAGGTGGT AGTTGATGGT TCTGGGCAGT GCCATTCTAC AGATACTGTG 
AAGAACACAC TTGATCCAAA GTGGAATCAG CATTATGACC TGTATATCGG AAAGTCTGAT 
TCAGTTACGA TCAGCGTATG GAATCATAAG AAGATCCATA AGAAGCAAGG TGCTGGATTT 
CTTGGTTGTG TTCGTCTTCT TTCCAATGCC ATCAACCGCC TCAAGGACAC TGGTTATCAG 
AGGTTGGATT TATGCAAACT GGGGCCAAAT GACAATGATA CAGTTCGAGG ACAAATTGTA 
GTAAGTCTTC AGTCCAGAGA CCGAATAGGC ACGGGAGGAC AAGTTGTGGA CTGTAGTCGT 
TTGTTTGACA ATGATTTGCC AGATGGTTGG GAAGAAAGGC GGACTGCCTC GGGAAGAATC 
CAGTATCTAA ACCACATCAC AAGAACTACA CAGTGGGAAC GCCCAACACG ACCGGCGTCA 
GAATATTCTA GTCCTGGCAG ACCCCTCAGC TGCTTTGTGG ATGAGAATAC TCCAATTACT 
GGAACAAATG GTGCAACGTG TGGACATTCT TCAGACCCCA GACTAGCAGA GAGAAGAGTC 
AGGTCCCAGC GACATAGAAA TTACATGAGC AGGACACACT TACACACTCC TCCAGACCTA 
CCGGAAGGCT ATGAACAAAG GACAACGCAG CAGGGTCAGG TATATTTCTT ACATACTCAG 
ACTGGTGTGA GCACATGGCA TGATCCACGA GTGCCTAGGG ATCTTAGCAA CATCAATTGT 
GAAGAGCTCG GTCCTTTGCC TCCTGGATGG GAGATCCGCA ATACCGCAAC AGGAAGAGTT 
TATTTCGTTG ACCATAACAA CAGAACAACA CAATTTACAG ATCCCCGGCT CTCTGCTAAC 
TTGCATTTAG TTTTAAATCG TCAGAACCAG TTGAAAGACC AACAGCAACA GCAAGTGGTG 
CCATTGTGTC CCGATGACAC TGAGTGTCTG ACAGTGCCAA GATACAAGCG AGATTTGGTT 
CAAAAACTAA AAATCTTGCG GCAAGAACTT TCCCAACAGC AGCCTCAAGC TGGCCACTGC 
CGTATTGAGG TGTCTAGGGA AGAGATTTTT GAGGAATCAT ATAGACAGGT CATGAAAATG 
AGGCCAAAAG ATCTATGGAA GCGATTAATG ATAAAATTTC GTGGAGAAGA AGGTCTTGAC 
TATGGAGGAG TTGCCAGGGA ATGGTTGTAT CTCCTGTCAC ATGAAATGTT GAATCCTTAC 
TATGGTCTCT TCCAGTATTC TAGAGATGAT ATCTACACAT TGCAGATCAA TCCTGATTCT 
GCAGTTAATC CGGAACATTT ATCCTATTTC CACTTTGTTG GACGGATAAT GGGAATGGCT 
GTATTCCATG GACATTATAT TGACGGTGGC TTCACATTAC CTTTTTATAA ACAGTTGCTT 
GGGAAGTCAA TTACTTTGGA TGACATGGAG CTAGTTGACC CAGACCTTCA TAATAGTTTA 
GTGTGGATAC TTGAAAACGA TATCACAGGT GTTCTGGACC ATACCTTCTG TGTTGAGCAT 
AATGCATATG GTGAAATAAT CCAGCATGAA CTCAAACCAA ATGGCAAAAG TATCCCTGTT 
ACTGAAGAAA ATAAAAAAGA ATATGTCAGG CTTTATGTCA ACTGGAGATT TCTCCGAGGC 
ATCGAAGCTC AGTTCCTGGC GCTGCAGAAG GGCTTTAATG AAGTCATTCC CCAGCACCTG 
CTGAAGACAT TTGATGAGAA GGAGCTGGAG CTCATTATTT GTGGACTTGG CAAAATTGAT 
GTGAGTGACT GGAAGGTCAA CACCCGGTTA AAACACTGTA CCCCAGACAG CAATGTCGTC 
AAGTGGTTCT GGAAAGCGGT GGAGTTTTTT GATGAAGAGA GACGAGCACG GTTGCTCCAG 
TTTGTGACAG GGTCCTCTCG AGTGCCTCTG CAGGGCTTCA AAGCACTACA AGGTGCTGCA 
GGCCCACGGC TCTTTACCAT ACACCAGATT GATGCCTGCA CGAACAACTT GCCAAAAGCC 
CACACTTGCT TCAATCGAAT AGACATTCCA CCCTATGAAA GCTATGAGAA GCTCTATGAA 
AAGCTGCTAA CGGCCATCGA GGAGACATGT GGCTTTGCTG TGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006533999.1
CDS	487..2694
Translation	MSNPGGRRNGPVKLRLTGLPDPFAKVVVDGSGQCHSTDTVKNTLDPKWNQHYDLYIGKSDSVTISVWNHKKIHKKQGAGFLGCVRLLSNAINRLKDTGYQRLDLCKLGPNDNDTVRGQIVVSLQSRDRIGTGGQVVDCSRLFDNDLPDGWEERRTASGRIQYLNHITRTTQWERPTRPASEYSSPGRPLSCFVDENTPITGTNGATCGHSSDPRLAERRVRSQRHRNYMSRTHLHTPPDLPEGYEQRTTQQGQVYFLHTQTGVSTWHDPRVPRDLSNINCEELGPLPPGWEIRNTATGRVYFVDHNNRTTQFTDPRLSANLHLVLNRQNQLKDQQQQQVVPLCPDDTECLTVPRYKRDLVQKLKILRQELSQQQPQAGHCRIEVSREEIFEESYRQVMKMRPKDLWKRLMIKFRGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSRDDIYTLQINPDSAVNPEHLSYFHFVGRIMGMAVFHGHYIDGGFTLPFYKQLLGKSITLDDMELVDPDLHNSLVWILENDITGVLDHTFCVEHNAYGEIIQHELKPNGKSIPVTEENKKEYVRLYVNWRFLRGIEAQFLALQKGFNEVIPQHLLKTFDEKELELIICGLGKIDVSDWKVNTRLKHCTPDSNVVKWFWKAVEFFDEERRARLLQFVTGSSRVPLQGFKALQGAAGPRLFTIHQIDACTNNLPKAHTCFNRIDIPPYESYEKLYEKLLTAIEETCGFAVE

Target ORF information:

RefSeq Version	XM_006533936.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus SMAD specific E3 ubiquitin protein ligase 2 (Smurf2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006533936.4

ORF Insert Sequence:

ATGTCTAACC CCGGAGGCCG GAGGAACGGG CCCGTCAAGC TGCGCCTGAC AGGTCTTCCT 
GATCCATTTG CTAAGGTGGT AGTTGATGGT TCTGGGCAGT GCCATTCTAC AGATACTGTG 
AAGAACACAC TTGATCCAAA GTGGAATCAG CATTATGACC TGTATATCGG AAAGTCTGAT 
TCAGTTACGA TCAGCGTATG GAATCATAAG AAGATCCATA AGAAGCAAGG TGCTGGATTT 
CTTGGTTGTG TTCGTCTTCT TTCCAATGCC ATCAACCGCC TCAAGGACAC TGGTTATCAG 
AGGTTGGATT TATGCAAACT GGGGCCAAAT GACAATGATA CAGTTCGAGG ACAAATTGTA 
GTAAGTCTTC AGTCCAGAGA CCGAATAGGC ACGGGAGGAC AAGTTGTGGA CTGTAGTCGT 
TTGTTTGACA ATGATTTGCC AGATGGTTGG GAAGAAAGGC GGACTGCCTC GGGAAGAATC 
CAGTATCTAA ACCACATCAC AAGAACTACA CAGTGGGAAC GCCCAACACG ACCGGCGTCA 
GAATATTCTA GTCCTGGCAG ACCCCTCAGC TGCTTTGTGG ATGAGAATAC TCCAATTACT 
GGAACAAATG GTGCAACGTG TGGACATTCT TCAGACCCCA GACTAGCAGA GAGAAGAGTC 
AGGTCCCAGC GACATAGAAA TTACATGAGC AGGACACACT TACACACTCC TCCAGACCTA 
CCGGAAGGCT ATGAACAAAG GACAACGCAG CAGGGTCAGG TATATTTCTT ACATACTCAG 
ACTGGTGTGA GCACATGGCA TGATCCACGA GTGCCTAGGG ATCTTAGCAA CATCAATTGT 
GAAGAGCTCG GTCCTTTGCC TCCTGGATGG GAGATCCGCA ATACCGCAAC AGGAAGAGTT 
TATTTCGTTG ACCATAACAA CAGAACAACA CAATTTACAG ATCCCCGGCT CTCTGCTAAC 
TTGCATTTAG TTTTAAATCG TCAGAACCAG TTGAAAGACC AACAGCAACA GCAAGTGGTG 
CCATTGTGTC CCGATGACAC TGAGTGTCTG ACAGTGCCAA GATACAAGCG AGATTTGGTT 
CAAAAACTAA AAATCTTGCG GCAAGAACTT TCCCAACAGC AGCCTCAAGC TGGCCACTGC 
CGTATTGAGG TGTCTAGGGA AGAGATTTTT GAGGAATCAT ATAGACAGGT CATGAAAATG 
AGGCCAAAAG ATCTATGGAA GCGATTAATG ATAAAATTTC GTGGAGAAGA AGGTCTTGAC 
TATGGAGGAG TTGCCAGGGA ATGGTTGTAT CTCCTGTCAC ATGAAATGTT GAATCCTTAC 
TATGGTCTCT TCCAGTATTC TAGAGATGAT ATCTACACAT TGCAGATCAA TCCTGATTCT 
GCAGTTAATC CGGAACATTT ATCCTATTTC CACTTTGTTG GACGGATAAT GGGAATGGCT 
GTATTCCATG GACATTATAT TGACGGTGGC TTCACATTAC CTTTTTATAA ACAGTTGCTT 
GGGAAGTCAA TTACTTTGGA TGACATGGAG CTAGTTGACC CAGACCTTCA TAATAGTTTA 
GTGTGGATAC TTGAAAACGA TATCACAGGT GTTCTGGACC ATACCTTCTG TGTTGAGCAT 
AATGCATATG GTGAAATAAT CCAGCATGAA CTCAAACCAA ATGGCAAAAG TATCCCTGTT 
ACTGAAGAAA ATAAAAAAGA ATATGTCAGG CTTTATGTCA ACTGGAGATT TCTCCGAGGC 
ATCGAAGCTC AGTTCCTGGC GCTGCAGAAG GGCTTTAATG AAGTCATTCC CCAGCACCTG 
CTGAAGACAT TTGATGAGAA GGAGCTGGAG CTCATTATTT GTGGACTTGG CAAAATTGAT 
GTGAGTGACT GGAAGGTCAA CACCCGGTTA AAACACTGTA CCCCAGACAG CAATGTCGTC 
AAGTGGTTCT GGAAAGCGGT GGAGTTTTTT GATGAAGAGA GACGAGCACG GTTGCTCCAG 
TTTGTGACAG GGTCCTCTCG AGTGCCTCTG CAGGGCTTCA AAGCACTACA AGGTGCTGCA 
GGCCCACGGC TCTTTACCAT ACACCAGATT GATGCCTGCA CGAACAACTT GCCAAAAGCC 
CACACTTGCT TCAATCGAAT AGACATTCCA CCCTATGAAA GCTATGAGAA GCTCTATGAA 
AAGCTGCTAA CGGCCATCGA GGAGACATGT GGCTTTGCTG TGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Smurf2-Mediated Stabilization of DNA Topoisomerase II? Controls Genomic Integrity.
Cancer research77(16)4217-4227(2017 Aug)
Emanuelli A,Borroni AP,Apel-Sarid L,Shah PA,Ayyathan DM,Koganti P,Levy-Cohen G,Blank M

USP4 inhibits SMAD4 monoubiquitination and promotes activin and BMP signaling.
The EMBO journal36(11)1623-1639(2017 06)
Zhou F,Xie F,Jin K,Zhang Z,Clerici M,Gao R,van Dinther M,Sixma TK,Huang H,Zhang L,Ten Dijke P

SMURF2 regulates bone homeostasis by disrupting SMAD3 interaction with vitamin D receptor in osteoblasts.
Nature communications814570(2017 Feb)
Xu Z,Greenblatt MB,Yan G,Feng H,Sun J,Lotinun S,Brady N,Baron R,Glimcher LH,Zou W

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Smurf2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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