Smurf2 cDNA ORF clone, Mus musculus(house mouse)

The following Smurf2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Smurf2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu46757 NM_001362894.1
Latest version!
Mus musculus SMAD specific E3 ubiquitin protein ligase 2 (Smurf2), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OMu46757 XM_017314705.1
Latest version!
Mus musculus SMAD specific E3 ubiquitin protein ligase 2 (Smurf2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OMu19698 NM_025481.3
Latest version!
Mus musculus SMAD specific E3 ubiquitin protein ligase 2 (Smurf2), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OMu46755 XM_006533936.4
Latest version!
Mus musculus SMAD specific E3 ubiquitin protein ligase 2 (Smurf2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
View Old Accession Versions >>
OMu19698 NM_025481.2 Mus musculus SMAD specific E3 ubiquitin protein ligase 2 (Smurf2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OMu46755 XM_006533936.3 Mus musculus SMAD specific E3 ubiquitin protein ligase 2 (Smurf2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu46757
    Clone ID Related Accession (Same CDS sequence) XM_017314705.1 , NM_001362894.1
    Accession Version NM_001362894.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1524bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-23
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase SMURF2 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL593847.36. On May 3, 2018 this sequence version replaced XM_017314705.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660813.107353.1, SRR1660811.196578.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGAGCAGGA CACACTTACA CACTCCTCCA GACCTACCGG AAGGCTATGA ACAAAGGACA 
    ACGCAGCAGG GTCAGGTATA TTTCTTACAT ACTCAGACTG GTGTGAGCAC ATGGCATGAT
    CCACGAGTGC CTAGGGATCT TAGCAACATC AATTGTGAAG AGCTCGGTCC TTTGCCTCCT
    GGATGGGAGA TCCGCAATAC CGCAACAGGA AGAGTTTATT TCGTTGACCA TAACAACAGA
    ACAACACAAT TTACAGATCC CCGGCTCTCT GCTAACTTGC ATTTAGTTTT AAATCGTCAG
    AACCAGTTGA AAGACCAACA GCAACAGCAA GTGGTGCCAT TGTGTCCCGA TGACACTGAG
    TGTCTGACAG TGCCAAGATA CAAGCGAGAT TTGGTTCAAA AACTAAAAAT CTTGCGGCAA
    GAACTTTCCC AACAGCAGCC TCAAGCTGGC CACTGCCGTA TTGAGGTGTC TAGGGAAGAG
    ATTTTTGAGG AATCATATAG ACAGGTCATG AAAATGAGGC CAAAAGATCT ATGGAAGCGA
    TTAATGATAA AATTTCGTGG AGAAGAAGGT CTTGACTATG GAGGAGTTGC CAGGGAATGG
    TTGTATCTCC TGTCACATGA AATGTTGAAT CCTTACTATG GTCTCTTCCA GTATTCTAGA
    GATGATATCT ACACATTGCA GATCAATCCT GATTCTGCAG TTAATCCGGA ACATTTATCC
    TATTTCCACT TTGTTGGACG GATAATGGGA ATGGCTGTAT TCCATGGACA TTATATTGAC
    GGTGGCTTCA CATTACCTTT TTATAAACAG TTGCTTGGGA AGTCAATTAC TTTGGATGAC
    ATGGAGCTAG TTGACCCAGA CCTTCATAAT AGTTTAGTGT GGATACTTGA AAACGATATC
    ACAGGTGTTC TGGACCATAC CTTCTGTGTT GAGCATAATG CATATGGTGA AATAATCCAG
    CATGAACTCA AACCAAATGG CAAAAGTATC CCTGTTACTG AAGAAAATAA AAAAGAATAT
    GTCAGGCTTT ATGTCAACTG GAGATTTCTC CGAGGCATCG AAGCTCAGTT CCTGGCGCTG
    CAGAAGGGCT TTAATGAAGT CATTCCCCAG CACCTGCTGA AGACATTTGA TGAGAAGGAG
    CTGGAGCTCA TTATTTGTGG ACTTGGCAAA ATTGATGTGA GTGACTGGAA GGTCAACACC
    CGGTTAAAAC ACTGTACCCC AGACAGCAAT GTCGTCAAGT GGTTCTGGAA AGCGGTGGAG
    TTTTTTGATG AAGAGAGACG AGCACGGTTG CTCCAGTTTG TGACAGGGTC CTCTCGAGTG
    CCTCTGCAGG GCTTCAAAGC ACTACAAGGT GCTGCAGGCC CACGGCTCTT TACCATACAC
    CAGATTGATG CCTGCACGAA CAACTTGCCA AAAGCCCACA CTTGCTTCAA TCGAATAGAC
    ATTCCACCCT ATGAAAGCTA TGAGAAGCTC TATGAAAAGC TGCTAACGGC CATCGAGGAG
    ACATGTGGCT TTGCTGTGGA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001349823.1
    CDS1319..2842
    Translation

    Target ORF information:

    RefSeq Version NM_001362894.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus SMAD specific E3 ubiquitin protein ligase 2 (Smurf2), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001362894.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGAGCAGGA CACACTTACA CACTCCTCCA GACCTACCGG AAGGCTATGA ACAAAGGACA 
    ACGCAGCAGG GTCAGGTATA TTTCTTACAT ACTCAGACTG GTGTGAGCAC ATGGCATGAT
    CCACGAGTGC CTAGGGATCT TAGCAACATC AATTGTGAAG AGCTCGGTCC TTTGCCTCCT
    GGATGGGAGA TCCGCAATAC CGCAACAGGA AGAGTTTATT TCGTTGACCA TAACAACAGA
    ACAACACAAT TTACAGATCC CCGGCTCTCT GCTAACTTGC ATTTAGTTTT AAATCGTCAG
    AACCAGTTGA AAGACCAACA GCAACAGCAA GTGGTGCCAT TGTGTCCCGA TGACACTGAG
    TGTCTGACAG TGCCAAGATA CAAGCGAGAT TTGGTTCAAA AACTAAAAAT CTTGCGGCAA
    GAACTTTCCC AACAGCAGCC TCAAGCTGGC CACTGCCGTA TTGAGGTGTC TAGGGAAGAG
    ATTTTTGAGG AATCATATAG ACAGGTCATG AAAATGAGGC CAAAAGATCT ATGGAAGCGA
    TTAATGATAA AATTTCGTGG AGAAGAAGGT CTTGACTATG GAGGAGTTGC CAGGGAATGG
    TTGTATCTCC TGTCACATGA AATGTTGAAT CCTTACTATG GTCTCTTCCA GTATTCTAGA
    GATGATATCT ACACATTGCA GATCAATCCT GATTCTGCAG TTAATCCGGA ACATTTATCC
    TATTTCCACT TTGTTGGACG GATAATGGGA ATGGCTGTAT TCCATGGACA TTATATTGAC
    GGTGGCTTCA CATTACCTTT TTATAAACAG TTGCTTGGGA AGTCAATTAC TTTGGATGAC
    ATGGAGCTAG TTGACCCAGA CCTTCATAAT AGTTTAGTGT GGATACTTGA AAACGATATC
    ACAGGTGTTC TGGACCATAC CTTCTGTGTT GAGCATAATG CATATGGTGA AATAATCCAG
    CATGAACTCA AACCAAATGG CAAAAGTATC CCTGTTACTG AAGAAAATAA AAAAGAATAT
    GTCAGGCTTT ATGTCAACTG GAGATTTCTC CGAGGCATCG AAGCTCAGTT CCTGGCGCTG
    CAGAAGGGCT TTAATGAAGT CATTCCCCAG CACCTGCTGA AGACATTTGA TGAGAAGGAG
    CTGGAGCTCA TTATTTGTGG ACTTGGCAAA ATTGATGTGA GTGACTGGAA GGTCAACACC
    CGGTTAAAAC ACTGTACCCC AGACAGCAAT GTCGTCAAGT GGTTCTGGAA AGCGGTGGAG
    TTTTTTGATG AAGAGAGACG AGCACGGTTG CTCCAGTTTG TGACAGGGTC CTCTCGAGTG
    CCTCTGCAGG GCTTCAAAGC ACTACAAGGT GCTGCAGGCC CACGGCTCTT TACCATACAC
    CAGATTGATG CCTGCACGAA CAACTTGCCA AAAGCCCACA CTTGCTTCAA TCGAATAGAC
    ATTCCACCCT ATGAAAGCTA TGAGAAGCTC TATGAAAAGC TGCTAACGGC CATCGAGGAG
    ACATGTGGCT TTGCTGTGGA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu46757
    Clone ID Related Accession (Same CDS sequence) XM_017314705.1 , NM_001362894.1
    Accession Version XM_017314705.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1524bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase SMURF2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGAGCAGGA CACACTTACA CACTCCTCCA GACCTACCGG AAGGCTATGA ACAAAGGACA 
    ACGCAGCAGG GTCAGGTATA TTTCTTACAT ACTCAGACTG GTGTGAGCAC ATGGCATGAT
    CCACGAGTGC CTAGGGATCT TAGCAACATC AATTGTGAAG AGCTCGGTCC TTTGCCTCCT
    GGATGGGAGA TCCGCAATAC CGCAACAGGA AGAGTTTATT TCGTTGACCA TAACAACAGA
    ACAACACAAT TTACAGATCC CCGGCTCTCT GCTAACTTGC ATTTAGTTTT AAATCGTCAG
    AACCAGTTGA AAGACCAACA GCAACAGCAA GTGGTGCCAT TGTGTCCCGA TGACACTGAG
    TGTCTGACAG TGCCAAGATA CAAGCGAGAT TTGGTTCAAA AACTAAAAAT CTTGCGGCAA
    GAACTTTCCC AACAGCAGCC TCAAGCTGGC CACTGCCGTA TTGAGGTGTC TAGGGAAGAG
    ATTTTTGAGG AATCATATAG ACAGGTCATG AAAATGAGGC CAAAAGATCT ATGGAAGCGA
    TTAATGATAA AATTTCGTGG AGAAGAAGGT CTTGACTATG GAGGAGTTGC CAGGGAATGG
    TTGTATCTCC TGTCACATGA AATGTTGAAT CCTTACTATG GTCTCTTCCA GTATTCTAGA
    GATGATATCT ACACATTGCA GATCAATCCT GATTCTGCAG TTAATCCGGA ACATTTATCC
    TATTTCCACT TTGTTGGACG GATAATGGGA ATGGCTGTAT TCCATGGACA TTATATTGAC
    GGTGGCTTCA CATTACCTTT TTATAAACAG TTGCTTGGGA AGTCAATTAC TTTGGATGAC
    ATGGAGCTAG TTGACCCAGA CCTTCATAAT AGTTTAGTGT GGATACTTGA AAACGATATC
    ACAGGTGTTC TGGACCATAC CTTCTGTGTT GAGCATAATG CATATGGTGA AATAATCCAG
    CATGAACTCA AACCAAATGG CAAAAGTATC CCTGTTACTG AAGAAAATAA AAAAGAATAT
    GTCAGGCTTT ATGTCAACTG GAGATTTCTC CGAGGCATCG AAGCTCAGTT CCTGGCGCTG
    CAGAAGGGCT TTAATGAAGT CATTCCCCAG CACCTGCTGA AGACATTTGA TGAGAAGGAG
    CTGGAGCTCA TTATTTGTGG ACTTGGCAAA ATTGATGTGA GTGACTGGAA GGTCAACACC
    CGGTTAAAAC ACTGTACCCC AGACAGCAAT GTCGTCAAGT GGTTCTGGAA AGCGGTGGAG
    TTTTTTGATG AAGAGAGACG AGCACGGTTG CTCCAGTTTG TGACAGGGTC CTCTCGAGTG
    CCTCTGCAGG GCTTCAAAGC ACTACAAGGT GCTGCAGGCC CACGGCTCTT TACCATACAC
    CAGATTGATG CCTGCACGAA CAACTTGCCA AAAGCCCACA CTTGCTTCAA TCGAATAGAC
    ATTCCACCCT ATGAAAGCTA TGAGAAGCTC TATGAAAAGC TGCTAACGGC CATCGAGGAG
    ACATGTGGCT TTGCTGTGGA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017170194.1
    CDS887..2410
    Translation

    Target ORF information:

    RefSeq Version XM_017314705.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus SMAD specific E3 ubiquitin protein ligase 2 (Smurf2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017314705.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGAGCAGGA CACACTTACA CACTCCTCCA GACCTACCGG AAGGCTATGA ACAAAGGACA 
    ACGCAGCAGG GTCAGGTATA TTTCTTACAT ACTCAGACTG GTGTGAGCAC ATGGCATGAT
    CCACGAGTGC CTAGGGATCT TAGCAACATC AATTGTGAAG AGCTCGGTCC TTTGCCTCCT
    GGATGGGAGA TCCGCAATAC CGCAACAGGA AGAGTTTATT TCGTTGACCA TAACAACAGA
    ACAACACAAT TTACAGATCC CCGGCTCTCT GCTAACTTGC ATTTAGTTTT AAATCGTCAG
    AACCAGTTGA AAGACCAACA GCAACAGCAA GTGGTGCCAT TGTGTCCCGA TGACACTGAG
    TGTCTGACAG TGCCAAGATA CAAGCGAGAT TTGGTTCAAA AACTAAAAAT CTTGCGGCAA
    GAACTTTCCC AACAGCAGCC TCAAGCTGGC CACTGCCGTA TTGAGGTGTC TAGGGAAGAG
    ATTTTTGAGG AATCATATAG ACAGGTCATG AAAATGAGGC CAAAAGATCT ATGGAAGCGA
    TTAATGATAA AATTTCGTGG AGAAGAAGGT CTTGACTATG GAGGAGTTGC CAGGGAATGG
    TTGTATCTCC TGTCACATGA AATGTTGAAT CCTTACTATG GTCTCTTCCA GTATTCTAGA
    GATGATATCT ACACATTGCA GATCAATCCT GATTCTGCAG TTAATCCGGA ACATTTATCC
    TATTTCCACT TTGTTGGACG GATAATGGGA ATGGCTGTAT TCCATGGACA TTATATTGAC
    GGTGGCTTCA CATTACCTTT TTATAAACAG TTGCTTGGGA AGTCAATTAC TTTGGATGAC
    ATGGAGCTAG TTGACCCAGA CCTTCATAAT AGTTTAGTGT GGATACTTGA AAACGATATC
    ACAGGTGTTC TGGACCATAC CTTCTGTGTT GAGCATAATG CATATGGTGA AATAATCCAG
    CATGAACTCA AACCAAATGG CAAAAGTATC CCTGTTACTG AAGAAAATAA AAAAGAATAT
    GTCAGGCTTT ATGTCAACTG GAGATTTCTC CGAGGCATCG AAGCTCAGTT CCTGGCGCTG
    CAGAAGGGCT TTAATGAAGT CATTCCCCAG CACCTGCTGA AGACATTTGA TGAGAAGGAG
    CTGGAGCTCA TTATTTGTGG ACTTGGCAAA ATTGATGTGA GTGACTGGAA GGTCAACACC
    CGGTTAAAAC ACTGTACCCC AGACAGCAAT GTCGTCAAGT GGTTCTGGAA AGCGGTGGAG
    TTTTTTGATG AAGAGAGACG AGCACGGTTG CTCCAGTTTG TGACAGGGTC CTCTCGAGTG
    CCTCTGCAGG GCTTCAAAGC ACTACAAGGT GCTGCAGGCC CACGGCTCTT TACCATACAC
    CAGATTGATG CCTGCACGAA CAACTTGCCA AAAGCCCACA CTTGCTTCAA TCGAATAGAC
    ATTCCACCCT ATGAAAGCTA TGAGAAGCTC TATGAAAAGC TGCTAACGGC CATCGAGGAG
    ACATGTGGCT TTGCTGTGGA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu19698
    Clone ID Related Accession (Same CDS sequence) NM_025481.2 , NM_025481.3
    Accession Version NM_025481.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2247bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-11-27
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase SMURF2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK012060.1, BU058366.1, AK013082.1, BU614468.1, AK036718.1 and BE986450.1. On Jul 31, 2009 this sequence version replaced NM_025481.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660819.150791.1, SRR1660811.163042.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCTAACC CCGGAGGCCG GAGGAACGGG CCCGTCAAGC TGCGCCTGAC AGTACTCTGT 
    GCAAAAAACT TGGTGAAAAA GGATTTTTTC CGTCTTCCTG ATCCATTTGC TAAGGTGGTA
    GTTGATGGTT CTGGGCAGTG CCATTCTACA GATACTGTGA AGAACACACT TGATCCAAAG
    TGGAATCAGC ATTATGACCT GTATATCGGA AAGTCTGATT CAGTTACGAT CAGCGTATGG
    AATCATAAGA AGATCCATAA GAAGCAAGGT GCTGGATTTC TTGGTTGTGT TCGTCTTCTT
    TCCAATGCCA TCAACCGCCT CAAGGACACT GGTTATCAGA GGTTGGATTT ATGCAAACTG
    GGGCCAAATG ACAATGATAC AGTTCGAGGA CAAATTGTAG TAAGTCTTCA GTCCAGAGAC
    CGAATAGGCA CGGGAGGACA AGTTGTGGAC TGTAGTCGTT TGTTTGACAA TGATTTGCCA
    GATGGTTGGG AAGAAAGGCG GACTGCCTCG GGAAGAATCC AGTATCTAAA CCACATCACA
    AGAACTACAC AGTGGGAACG CCCAACACGA CCGGCGTCAG AATATTCTAG TCCTGGCAGA
    CCCCTCAGCT GCTTTGTGGA TGAGAATACT CCAATTACTG GAACAAATGG TGCAACGTGT
    GGACATTCTT CAGACCCCAG ACTAGCAGAG AGAAGAGTCA GGTCCCAGCG ACATAGAAAT
    TACATGAGCA GGACACACTT ACACACTCCT CCAGACCTAC CGGAAGGCTA TGAACAAAGG
    ACAACGCAGC AGGGTCAGGT ATATTTCTTA CATACTCAGA CTGGTGTGAG CACATGGCAT
    GATCCACGAG TGCCTAGGGA TCTTAGCAAC ATCAATTGTG AAGAGCTCGG TCCTTTGCCT
    CCTGGATGGG AGATCCGCAA TACCGCAACA GGAAGAGTTT ATTTCGTTGA CCATAACAAC
    AGAACAACAC AATTTACAGA TCCCCGGCTC TCTGCTAACT TGCATTTAGT TTTAAATCGT
    CAGAACCAGT TGAAAGACCA ACAGCAACAG CAAGTGGTGC CATTGTGTCC CGATGACACT
    GAGTGTCTGA CAGTGCCAAG ATACAAGCGA GATTTGGTTC AAAAACTAAA AATCTTGCGG
    CAAGAACTTT CCCAACAGCA GCCTCAAGCT GGCCACTGCC GTATTGAGGT GTCTAGGGAA
    GAGATTTTTG AGGAATCATA TAGACAGGTC ATGAAAATGA GGCCAAAAGA TCTATGGAAG
    CGATTAATGA TAAAATTTCG TGGAGAAGAA GGTCTTGACT ATGGAGGAGT TGCCAGGGAA
    TGGTTGTATC TCCTGTCACA TGAAATGTTG AATCCTTACT ATGGTCTCTT CCAGTATTCT
    AGAGATGATA TCTACACATT GCAGATCAAT CCTGATTCTG CAGTTAATCC GGAACATTTA
    TCCTATTTCC ACTTTGTTGG ACGGATAATG GGAATGGCTG TATTCCATGG ACATTATATT
    GACGGTGGCT TCACATTACC TTTTTATAAA CAGTTGCTTG GGAAGTCAAT TACTTTGGAT
    GACATGGAGC TAGTTGACCC AGACCTTCAT AATAGTTTAG TGTGGATACT TGAAAACGAT
    ATCACAGGTG TTCTGGACCA TACCTTCTGT GTTGAGCATA ATGCATATGG TGAAATAATC
    CAGCATGAAC TCAAACCAAA TGGCAAAAGT ATCCCTGTTA CTGAAGAAAA TAAAAAAGAA
    TATGTCAGGC TTTATGTCAA CTGGAGATTT CTCCGAGGCA TCGAAGCTCA GTTCCTGGCG
    CTGCAGAAGG GCTTTAATGA AGTCATTCCC CAGCACCTGC TGAAGACATT TGATGAGAAG
    GAGCTGGAGC TCATTATTTG TGGACTTGGC AAAATTGATG TGAGTGACTG GAAGGTCAAC
    ACCCGGTTAA AACACTGTAC CCCAGACAGC AATGTCGTCA AGTGGTTCTG GAAAGCGGTG
    GAGTTTTTTG ATGAAGAGAG ACGAGCACGG TTGCTCCAGT TTGTGACAGG GTCCTCTCGA
    GTGCCTCTGC AGGGCTTCAA AGCACTACAA GGTGCTGCAG GCCCACGGCT CTTTACCATA
    CACCAGATTG ATGCCTGCAC GAACAACTTG CCAAAAGCCC ACACTTGCTT CAATCGAATA
    GACATTCCAC CCTATGAAAG CTATGAGAAG CTCTATGAAA AGCTGCTAAC GGCCATCGAG
    GAGACATGTG GCTTTGCTGT GGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_079757.2
    CDS484..2730
    Translation

    Target ORF information:

    RefSeq Version NM_025481.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus SMAD specific E3 ubiquitin protein ligase 2 (Smurf2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_025481.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCTAACC CCGGAGGCCG GAGGAACGGG CCCGTCAAGC TGCGCCTGAC AGTACTCTGT 
    GCAAAAAACT TGGTGAAAAA GGATTTTTTC CGTCTTCCTG ATCCATTTGC TAAGGTGGTA
    GTTGATGGTT CTGGGCAGTG CCATTCTACA GATACTGTGA AGAACACACT TGATCCAAAG
    TGGAATCAGC ATTATGACCT GTATATCGGA AAGTCTGATT CAGTTACGAT CAGCGTATGG
    AATCATAAGA AGATCCATAA GAAGCAAGGT GCTGGATTTC TTGGTTGTGT TCGTCTTCTT
    TCCAATGCCA TCAACCGCCT CAAGGACACT GGTTATCAGA GGTTGGATTT ATGCAAACTG
    GGGCCAAATG ACAATGATAC AGTTCGAGGA CAAATTGTAG TAAGTCTTCA GTCCAGAGAC
    CGAATAGGCA CGGGAGGACA AGTTGTGGAC TGTAGTCGTT TGTTTGACAA TGATTTGCCA
    GATGGTTGGG AAGAAAGGCG GACTGCCTCG GGAAGAATCC AGTATCTAAA CCACATCACA
    AGAACTACAC AGTGGGAACG CCCAACACGA CCGGCGTCAG AATATTCTAG TCCTGGCAGA
    CCCCTCAGCT GCTTTGTGGA TGAGAATACT CCAATTACTG GAACAAATGG TGCAACGTGT
    GGACATTCTT CAGACCCCAG ACTAGCAGAG AGAAGAGTCA GGTCCCAGCG ACATAGAAAT
    TACATGAGCA GGACACACTT ACACACTCCT CCAGACCTAC CGGAAGGCTA TGAACAAAGG
    ACAACGCAGC AGGGTCAGGT ATATTTCTTA CATACTCAGA CTGGTGTGAG CACATGGCAT
    GATCCACGAG TGCCTAGGGA TCTTAGCAAC ATCAATTGTG AAGAGCTCGG TCCTTTGCCT
    CCTGGATGGG AGATCCGCAA TACCGCAACA GGAAGAGTTT ATTTCGTTGA CCATAACAAC
    AGAACAACAC AATTTACAGA TCCCCGGCTC TCTGCTAACT TGCATTTAGT TTTAAATCGT
    CAGAACCAGT TGAAAGACCA ACAGCAACAG CAAGTGGTGC CATTGTGTCC CGATGACACT
    GAGTGTCTGA CAGTGCCAAG ATACAAGCGA GATTTGGTTC AAAAACTAAA AATCTTGCGG
    CAAGAACTTT CCCAACAGCA GCCTCAAGCT GGCCACTGCC GTATTGAGGT GTCTAGGGAA
    GAGATTTTTG AGGAATCATA TAGACAGGTC ATGAAAATGA GGCCAAAAGA TCTATGGAAG
    CGATTAATGA TAAAATTTCG TGGAGAAGAA GGTCTTGACT ATGGAGGAGT TGCCAGGGAA
    TGGTTGTATC TCCTGTCACA TGAAATGTTG AATCCTTACT ATGGTCTCTT CCAGTATTCT
    AGAGATGATA TCTACACATT GCAGATCAAT CCTGATTCTG CAGTTAATCC GGAACATTTA
    TCCTATTTCC ACTTTGTTGG ACGGATAATG GGAATGGCTG TATTCCATGG ACATTATATT
    GACGGTGGCT TCACATTACC TTTTTATAAA CAGTTGCTTG GGAAGTCAAT TACTTTGGAT
    GACATGGAGC TAGTTGACCC AGACCTTCAT AATAGTTTAG TGTGGATACT TGAAAACGAT
    ATCACAGGTG TTCTGGACCA TACCTTCTGT GTTGAGCATA ATGCATATGG TGAAATAATC
    CAGCATGAAC TCAAACCAAA TGGCAAAAGT ATCCCTGTTA CTGAAGAAAA TAAAAAAGAA
    TATGTCAGGC TTTATGTCAA CTGGAGATTT CTCCGAGGCA TCGAAGCTCA GTTCCTGGCG
    CTGCAGAAGG GCTTTAATGA AGTCATTCCC CAGCACCTGC TGAAGACATT TGATGAGAAG
    GAGCTGGAGC TCATTATTTG TGGACTTGGC AAAATTGATG TGAGTGACTG GAAGGTCAAC
    ACCCGGTTAA AACACTGTAC CCCAGACAGC AATGTCGTCA AGTGGTTCTG GAAAGCGGTG
    GAGTTTTTTG ATGAAGAGAG ACGAGCACGG TTGCTCCAGT TTGTGACAGG GTCCTCTCGA
    GTGCCTCTGC AGGGCTTCAA AGCACTACAA GGTGCTGCAG GCCCACGGCT CTTTACCATA
    CACCAGATTG ATGCCTGCAC GAACAACTTG CCAAAAGCCC ACACTTGCTT CAATCGAATA
    GACATTCCAC CCTATGAAAG CTATGAGAAG CTCTATGAAA AGCTGCTAAC GGCCATCGAG
    GAGACATGTG GCTTTGCTGT GGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu19698
    Clone ID Related Accession (Same CDS sequence) NM_025481.2 , NM_025481.3
    Accession Version NM_025481.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2247bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-07-22
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase SMURF2 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK012060.1, BU058366.1, AK013082.1, BU614468.1, AK036718.1, BE986450.1 and AL593847.36. On May 3, 2018 this sequence version replaced NM_025481.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660819.150791.1, SRR1660811.163042.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCTAACC CCGGAGGCCG GAGGAACGGG CCCGTCAAGC TGCGCCTGAC AGTACTCTGT 
    GCAAAAAACT TGGTGAAAAA GGATTTTTTC CGTCTTCCTG ATCCATTTGC TAAGGTGGTA
    GTTGATGGTT CTGGGCAGTG CCATTCTACA GATACTGTGA AGAACACACT TGATCCAAAG
    TGGAATCAGC ATTATGACCT GTATATCGGA AAGTCTGATT CAGTTACGAT CAGCGTATGG
    AATCATAAGA AGATCCATAA GAAGCAAGGT GCTGGATTTC TTGGTTGTGT TCGTCTTCTT
    TCCAATGCCA TCAACCGCCT CAAGGACACT GGTTATCAGA GGTTGGATTT ATGCAAACTG
    GGGCCAAATG ACAATGATAC AGTTCGAGGA CAAATTGTAG TAAGTCTTCA GTCCAGAGAC
    CGAATAGGCA CGGGAGGACA AGTTGTGGAC TGTAGTCGTT TGTTTGACAA TGATTTGCCA
    GATGGTTGGG AAGAAAGGCG GACTGCCTCG GGAAGAATCC AGTATCTAAA CCACATCACA
    AGAACTACAC AGTGGGAACG CCCAACACGA CCGGCGTCAG AATATTCTAG TCCTGGCAGA
    CCCCTCAGCT GCTTTGTGGA TGAGAATACT CCAATTACTG GAACAAATGG TGCAACGTGT
    GGACATTCTT CAGACCCCAG ACTAGCAGAG AGAAGAGTCA GGTCCCAGCG ACATAGAAAT
    TACATGAGCA GGACACACTT ACACACTCCT CCAGACCTAC CGGAAGGCTA TGAACAAAGG
    ACAACGCAGC AGGGTCAGGT ATATTTCTTA CATACTCAGA CTGGTGTGAG CACATGGCAT
    GATCCACGAG TGCCTAGGGA TCTTAGCAAC ATCAATTGTG AAGAGCTCGG TCCTTTGCCT
    CCTGGATGGG AGATCCGCAA TACCGCAACA GGAAGAGTTT ATTTCGTTGA CCATAACAAC
    AGAACAACAC AATTTACAGA TCCCCGGCTC TCTGCTAACT TGCATTTAGT TTTAAATCGT
    CAGAACCAGT TGAAAGACCA ACAGCAACAG CAAGTGGTGC CATTGTGTCC CGATGACACT
    GAGTGTCTGA CAGTGCCAAG ATACAAGCGA GATTTGGTTC AAAAACTAAA AATCTTGCGG
    CAAGAACTTT CCCAACAGCA GCCTCAAGCT GGCCACTGCC GTATTGAGGT GTCTAGGGAA
    GAGATTTTTG AGGAATCATA TAGACAGGTC ATGAAAATGA GGCCAAAAGA TCTATGGAAG
    CGATTAATGA TAAAATTTCG TGGAGAAGAA GGTCTTGACT ATGGAGGAGT TGCCAGGGAA
    TGGTTGTATC TCCTGTCACA TGAAATGTTG AATCCTTACT ATGGTCTCTT CCAGTATTCT
    AGAGATGATA TCTACACATT GCAGATCAAT CCTGATTCTG CAGTTAATCC GGAACATTTA
    TCCTATTTCC ACTTTGTTGG ACGGATAATG GGAATGGCTG TATTCCATGG ACATTATATT
    GACGGTGGCT TCACATTACC TTTTTATAAA CAGTTGCTTG GGAAGTCAAT TACTTTGGAT
    GACATGGAGC TAGTTGACCC AGACCTTCAT AATAGTTTAG TGTGGATACT TGAAAACGAT
    ATCACAGGTG TTCTGGACCA TACCTTCTGT GTTGAGCATA ATGCATATGG TGAAATAATC
    CAGCATGAAC TCAAACCAAA TGGCAAAAGT ATCCCTGTTA CTGAAGAAAA TAAAAAAGAA
    TATGTCAGGC TTTATGTCAA CTGGAGATTT CTCCGAGGCA TCGAAGCTCA GTTCCTGGCG
    CTGCAGAAGG GCTTTAATGA AGTCATTCCC CAGCACCTGC TGAAGACATT TGATGAGAAG
    GAGCTGGAGC TCATTATTTG TGGACTTGGC AAAATTGATG TGAGTGACTG GAAGGTCAAC
    ACCCGGTTAA AACACTGTAC CCCAGACAGC AATGTCGTCA AGTGGTTCTG GAAAGCGGTG
    GAGTTTTTTG ATGAAGAGAG ACGAGCACGG TTGCTCCAGT TTGTGACAGG GTCCTCTCGA
    GTGCCTCTGC AGGGCTTCAA AGCACTACAA GGTGCTGCAG GCCCACGGCT CTTTACCATA
    CACCAGATTG ATGCCTGCAC GAACAACTTG CCAAAAGCCC ACACTTGCTT CAATCGAATA
    GACATTCCAC CCTATGAAAG CTATGAGAAG CTCTATGAAA AGCTGCTAAC GGCCATCGAG
    GAGACATGTG GCTTTGCTGT GGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_079757.2
    CDS484..2730
    Translation

    Target ORF information:

    RefSeq Version NM_025481.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus SMAD specific E3 ubiquitin protein ligase 2 (Smurf2), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_025481.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCTAACC CCGGAGGCCG GAGGAACGGG CCCGTCAAGC TGCGCCTGAC AGTACTCTGT 
    GCAAAAAACT TGGTGAAAAA GGATTTTTTC CGTCTTCCTG ATCCATTTGC TAAGGTGGTA
    GTTGATGGTT CTGGGCAGTG CCATTCTACA GATACTGTGA AGAACACACT TGATCCAAAG
    TGGAATCAGC ATTATGACCT GTATATCGGA AAGTCTGATT CAGTTACGAT CAGCGTATGG
    AATCATAAGA AGATCCATAA GAAGCAAGGT GCTGGATTTC TTGGTTGTGT TCGTCTTCTT
    TCCAATGCCA TCAACCGCCT CAAGGACACT GGTTATCAGA GGTTGGATTT ATGCAAACTG
    GGGCCAAATG ACAATGATAC AGTTCGAGGA CAAATTGTAG TAAGTCTTCA GTCCAGAGAC
    CGAATAGGCA CGGGAGGACA AGTTGTGGAC TGTAGTCGTT TGTTTGACAA TGATTTGCCA
    GATGGTTGGG AAGAAAGGCG GACTGCCTCG GGAAGAATCC AGTATCTAAA CCACATCACA
    AGAACTACAC AGTGGGAACG CCCAACACGA CCGGCGTCAG AATATTCTAG TCCTGGCAGA
    CCCCTCAGCT GCTTTGTGGA TGAGAATACT CCAATTACTG GAACAAATGG TGCAACGTGT
    GGACATTCTT CAGACCCCAG ACTAGCAGAG AGAAGAGTCA GGTCCCAGCG ACATAGAAAT
    TACATGAGCA GGACACACTT ACACACTCCT CCAGACCTAC CGGAAGGCTA TGAACAAAGG
    ACAACGCAGC AGGGTCAGGT ATATTTCTTA CATACTCAGA CTGGTGTGAG CACATGGCAT
    GATCCACGAG TGCCTAGGGA TCTTAGCAAC ATCAATTGTG AAGAGCTCGG TCCTTTGCCT
    CCTGGATGGG AGATCCGCAA TACCGCAACA GGAAGAGTTT ATTTCGTTGA CCATAACAAC
    AGAACAACAC AATTTACAGA TCCCCGGCTC TCTGCTAACT TGCATTTAGT TTTAAATCGT
    CAGAACCAGT TGAAAGACCA ACAGCAACAG CAAGTGGTGC CATTGTGTCC CGATGACACT
    GAGTGTCTGA CAGTGCCAAG ATACAAGCGA GATTTGGTTC AAAAACTAAA AATCTTGCGG
    CAAGAACTTT CCCAACAGCA GCCTCAAGCT GGCCACTGCC GTATTGAGGT GTCTAGGGAA
    GAGATTTTTG AGGAATCATA TAGACAGGTC ATGAAAATGA GGCCAAAAGA TCTATGGAAG
    CGATTAATGA TAAAATTTCG TGGAGAAGAA GGTCTTGACT ATGGAGGAGT TGCCAGGGAA
    TGGTTGTATC TCCTGTCACA TGAAATGTTG AATCCTTACT ATGGTCTCTT CCAGTATTCT
    AGAGATGATA TCTACACATT GCAGATCAAT CCTGATTCTG CAGTTAATCC GGAACATTTA
    TCCTATTTCC ACTTTGTTGG ACGGATAATG GGAATGGCTG TATTCCATGG ACATTATATT
    GACGGTGGCT TCACATTACC TTTTTATAAA CAGTTGCTTG GGAAGTCAAT TACTTTGGAT
    GACATGGAGC TAGTTGACCC AGACCTTCAT AATAGTTTAG TGTGGATACT TGAAAACGAT
    ATCACAGGTG TTCTGGACCA TACCTTCTGT GTTGAGCATA ATGCATATGG TGAAATAATC
    CAGCATGAAC TCAAACCAAA TGGCAAAAGT ATCCCTGTTA CTGAAGAAAA TAAAAAAGAA
    TATGTCAGGC TTTATGTCAA CTGGAGATTT CTCCGAGGCA TCGAAGCTCA GTTCCTGGCG
    CTGCAGAAGG GCTTTAATGA AGTCATTCCC CAGCACCTGC TGAAGACATT TGATGAGAAG
    GAGCTGGAGC TCATTATTTG TGGACTTGGC AAAATTGATG TGAGTGACTG GAAGGTCAAC
    ACCCGGTTAA AACACTGTAC CCCAGACAGC AATGTCGTCA AGTGGTTCTG GAAAGCGGTG
    GAGTTTTTTG ATGAAGAGAG ACGAGCACGG TTGCTCCAGT TTGTGACAGG GTCCTCTCGA
    GTGCCTCTGC AGGGCTTCAA AGCACTACAA GGTGCTGCAG GCCCACGGCT CTTTACCATA
    CACCAGATTG ATGCCTGCAC GAACAACTTG CCAAAAGCCC ACACTTGCTT CAATCGAATA
    GACATTCCAC CCTATGAAAG CTATGAGAAG CTCTATGAAA AGCTGCTAAC GGCCATCGAG
    GAGACATGTG GCTTTGCTGT GGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu46755
    Clone ID Related Accession (Same CDS sequence) XM_006533936.4 , XM_006533936.3
    Accession Version XM_006533936.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2208bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase SMURF2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006533936.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCTAACC CCGGAGGCCG GAGGAACGGG CCCGTCAAGC TGCGCCTGAC AGGTCTTCCT 
    GATCCATTTG CTAAGGTGGT AGTTGATGGT TCTGGGCAGT GCCATTCTAC AGATACTGTG
    AAGAACACAC TTGATCCAAA GTGGAATCAG CATTATGACC TGTATATCGG AAAGTCTGAT
    TCAGTTACGA TCAGCGTATG GAATCATAAG AAGATCCATA AGAAGCAAGG TGCTGGATTT
    CTTGGTTGTG TTCGTCTTCT TTCCAATGCC ATCAACCGCC TCAAGGACAC TGGTTATCAG
    AGGTTGGATT TATGCAAACT GGGGCCAAAT GACAATGATA CAGTTCGAGG ACAAATTGTA
    GTAAGTCTTC AGTCCAGAGA CCGAATAGGC ACGGGAGGAC AAGTTGTGGA CTGTAGTCGT
    TTGTTTGACA ATGATTTGCC AGATGGTTGG GAAGAAAGGC GGACTGCCTC GGGAAGAATC
    CAGTATCTAA ACCACATCAC AAGAACTACA CAGTGGGAAC GCCCAACACG ACCGGCGTCA
    GAATATTCTA GTCCTGGCAG ACCCCTCAGC TGCTTTGTGG ATGAGAATAC TCCAATTACT
    GGAACAAATG GTGCAACGTG TGGACATTCT TCAGACCCCA GACTAGCAGA GAGAAGAGTC
    AGGTCCCAGC GACATAGAAA TTACATGAGC AGGACACACT TACACACTCC TCCAGACCTA
    CCGGAAGGCT ATGAACAAAG GACAACGCAG CAGGGTCAGG TATATTTCTT ACATACTCAG
    ACTGGTGTGA GCACATGGCA TGATCCACGA GTGCCTAGGG ATCTTAGCAA CATCAATTGT
    GAAGAGCTCG GTCCTTTGCC TCCTGGATGG GAGATCCGCA ATACCGCAAC AGGAAGAGTT
    TATTTCGTTG ACCATAACAA CAGAACAACA CAATTTACAG ATCCCCGGCT CTCTGCTAAC
    TTGCATTTAG TTTTAAATCG TCAGAACCAG TTGAAAGACC AACAGCAACA GCAAGTGGTG
    CCATTGTGTC CCGATGACAC TGAGTGTCTG ACAGTGCCAA GATACAAGCG AGATTTGGTT
    CAAAAACTAA AAATCTTGCG GCAAGAACTT TCCCAACAGC AGCCTCAAGC TGGCCACTGC
    CGTATTGAGG TGTCTAGGGA AGAGATTTTT GAGGAATCAT ATAGACAGGT CATGAAAATG
    AGGCCAAAAG ATCTATGGAA GCGATTAATG ATAAAATTTC GTGGAGAAGA AGGTCTTGAC
    TATGGAGGAG TTGCCAGGGA ATGGTTGTAT CTCCTGTCAC ATGAAATGTT GAATCCTTAC
    TATGGTCTCT TCCAGTATTC TAGAGATGAT ATCTACACAT TGCAGATCAA TCCTGATTCT
    GCAGTTAATC CGGAACATTT ATCCTATTTC CACTTTGTTG GACGGATAAT GGGAATGGCT
    GTATTCCATG GACATTATAT TGACGGTGGC TTCACATTAC CTTTTTATAA ACAGTTGCTT
    GGGAAGTCAA TTACTTTGGA TGACATGGAG CTAGTTGACC CAGACCTTCA TAATAGTTTA
    GTGTGGATAC TTGAAAACGA TATCACAGGT GTTCTGGACC ATACCTTCTG TGTTGAGCAT
    AATGCATATG GTGAAATAAT CCAGCATGAA CTCAAACCAA ATGGCAAAAG TATCCCTGTT
    ACTGAAGAAA ATAAAAAAGA ATATGTCAGG CTTTATGTCA ACTGGAGATT TCTCCGAGGC
    ATCGAAGCTC AGTTCCTGGC GCTGCAGAAG GGCTTTAATG AAGTCATTCC CCAGCACCTG
    CTGAAGACAT TTGATGAGAA GGAGCTGGAG CTCATTATTT GTGGACTTGG CAAAATTGAT
    GTGAGTGACT GGAAGGTCAA CACCCGGTTA AAACACTGTA CCCCAGACAG CAATGTCGTC
    AAGTGGTTCT GGAAAGCGGT GGAGTTTTTT GATGAAGAGA GACGAGCACG GTTGCTCCAG
    TTTGTGACAG GGTCCTCTCG AGTGCCTCTG CAGGGCTTCA AAGCACTACA AGGTGCTGCA
    GGCCCACGGC TCTTTACCAT ACACCAGATT GATGCCTGCA CGAACAACTT GCCAAAAGCC
    CACACTTGCT TCAATCGAAT AGACATTCCA CCCTATGAAA GCTATGAGAA GCTCTATGAA
    AAGCTGCTAA CGGCCATCGA GGAGACATGT GGCTTTGCTG TGGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006533999.1
    CDS478..2685
    Translation

    Target ORF information:

    RefSeq Version XM_006533936.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus SMAD specific E3 ubiquitin protein ligase 2 (Smurf2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006533936.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCTAACC CCGGAGGCCG GAGGAACGGG CCCGTCAAGC TGCGCCTGAC AGGTCTTCCT 
    GATCCATTTG CTAAGGTGGT AGTTGATGGT TCTGGGCAGT GCCATTCTAC AGATACTGTG
    AAGAACACAC TTGATCCAAA GTGGAATCAG CATTATGACC TGTATATCGG AAAGTCTGAT
    TCAGTTACGA TCAGCGTATG GAATCATAAG AAGATCCATA AGAAGCAAGG TGCTGGATTT
    CTTGGTTGTG TTCGTCTTCT TTCCAATGCC ATCAACCGCC TCAAGGACAC TGGTTATCAG
    AGGTTGGATT TATGCAAACT GGGGCCAAAT GACAATGATA CAGTTCGAGG ACAAATTGTA
    GTAAGTCTTC AGTCCAGAGA CCGAATAGGC ACGGGAGGAC AAGTTGTGGA CTGTAGTCGT
    TTGTTTGACA ATGATTTGCC AGATGGTTGG GAAGAAAGGC GGACTGCCTC GGGAAGAATC
    CAGTATCTAA ACCACATCAC AAGAACTACA CAGTGGGAAC GCCCAACACG ACCGGCGTCA
    GAATATTCTA GTCCTGGCAG ACCCCTCAGC TGCTTTGTGG ATGAGAATAC TCCAATTACT
    GGAACAAATG GTGCAACGTG TGGACATTCT TCAGACCCCA GACTAGCAGA GAGAAGAGTC
    AGGTCCCAGC GACATAGAAA TTACATGAGC AGGACACACT TACACACTCC TCCAGACCTA
    CCGGAAGGCT ATGAACAAAG GACAACGCAG CAGGGTCAGG TATATTTCTT ACATACTCAG
    ACTGGTGTGA GCACATGGCA TGATCCACGA GTGCCTAGGG ATCTTAGCAA CATCAATTGT
    GAAGAGCTCG GTCCTTTGCC TCCTGGATGG GAGATCCGCA ATACCGCAAC AGGAAGAGTT
    TATTTCGTTG ACCATAACAA CAGAACAACA CAATTTACAG ATCCCCGGCT CTCTGCTAAC
    TTGCATTTAG TTTTAAATCG TCAGAACCAG TTGAAAGACC AACAGCAACA GCAAGTGGTG
    CCATTGTGTC CCGATGACAC TGAGTGTCTG ACAGTGCCAA GATACAAGCG AGATTTGGTT
    CAAAAACTAA AAATCTTGCG GCAAGAACTT TCCCAACAGC AGCCTCAAGC TGGCCACTGC
    CGTATTGAGG TGTCTAGGGA AGAGATTTTT GAGGAATCAT ATAGACAGGT CATGAAAATG
    AGGCCAAAAG ATCTATGGAA GCGATTAATG ATAAAATTTC GTGGAGAAGA AGGTCTTGAC
    TATGGAGGAG TTGCCAGGGA ATGGTTGTAT CTCCTGTCAC ATGAAATGTT GAATCCTTAC
    TATGGTCTCT TCCAGTATTC TAGAGATGAT ATCTACACAT TGCAGATCAA TCCTGATTCT
    GCAGTTAATC CGGAACATTT ATCCTATTTC CACTTTGTTG GACGGATAAT GGGAATGGCT
    GTATTCCATG GACATTATAT TGACGGTGGC TTCACATTAC CTTTTTATAA ACAGTTGCTT
    GGGAAGTCAA TTACTTTGGA TGACATGGAG CTAGTTGACC CAGACCTTCA TAATAGTTTA
    GTGTGGATAC TTGAAAACGA TATCACAGGT GTTCTGGACC ATACCTTCTG TGTTGAGCAT
    AATGCATATG GTGAAATAAT CCAGCATGAA CTCAAACCAA ATGGCAAAAG TATCCCTGTT
    ACTGAAGAAA ATAAAAAAGA ATATGTCAGG CTTTATGTCA ACTGGAGATT TCTCCGAGGC
    ATCGAAGCTC AGTTCCTGGC GCTGCAGAAG GGCTTTAATG AAGTCATTCC CCAGCACCTG
    CTGAAGACAT TTGATGAGAA GGAGCTGGAG CTCATTATTT GTGGACTTGG CAAAATTGAT
    GTGAGTGACT GGAAGGTCAA CACCCGGTTA AAACACTGTA CCCCAGACAG CAATGTCGTC
    AAGTGGTTCT GGAAAGCGGT GGAGTTTTTT GATGAAGAGA GACGAGCACG GTTGCTCCAG
    TTTGTGACAG GGTCCTCTCG AGTGCCTCTG CAGGGCTTCA AAGCACTACA AGGTGCTGCA
    GGCCCACGGC TCTTTACCAT ACACCAGATT GATGCCTGCA CGAACAACTT GCCAAAAGCC
    CACACTTGCT TCAATCGAAT AGACATTCCA CCCTATGAAA GCTATGAGAA GCTCTATGAA
    AAGCTGCTAA CGGCCATCGA GGAGACATGT GGCTTTGCTG TGGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu46755
    Clone ID Related Accession (Same CDS sequence) XM_006533936.4 , XM_006533936.3
    Accession Version XM_006533936.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2208bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase SMURF2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006533936.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCTAACC CCGGAGGCCG GAGGAACGGG CCCGTCAAGC TGCGCCTGAC AGGTCTTCCT 
    GATCCATTTG CTAAGGTGGT AGTTGATGGT TCTGGGCAGT GCCATTCTAC AGATACTGTG
    AAGAACACAC TTGATCCAAA GTGGAATCAG CATTATGACC TGTATATCGG AAAGTCTGAT
    TCAGTTACGA TCAGCGTATG GAATCATAAG AAGATCCATA AGAAGCAAGG TGCTGGATTT
    CTTGGTTGTG TTCGTCTTCT TTCCAATGCC ATCAACCGCC TCAAGGACAC TGGTTATCAG
    AGGTTGGATT TATGCAAACT GGGGCCAAAT GACAATGATA CAGTTCGAGG ACAAATTGTA
    GTAAGTCTTC AGTCCAGAGA CCGAATAGGC ACGGGAGGAC AAGTTGTGGA CTGTAGTCGT
    TTGTTTGACA ATGATTTGCC AGATGGTTGG GAAGAAAGGC GGACTGCCTC GGGAAGAATC
    CAGTATCTAA ACCACATCAC AAGAACTACA CAGTGGGAAC GCCCAACACG ACCGGCGTCA
    GAATATTCTA GTCCTGGCAG ACCCCTCAGC TGCTTTGTGG ATGAGAATAC TCCAATTACT
    GGAACAAATG GTGCAACGTG TGGACATTCT TCAGACCCCA GACTAGCAGA GAGAAGAGTC
    AGGTCCCAGC GACATAGAAA TTACATGAGC AGGACACACT TACACACTCC TCCAGACCTA
    CCGGAAGGCT ATGAACAAAG GACAACGCAG CAGGGTCAGG TATATTTCTT ACATACTCAG
    ACTGGTGTGA GCACATGGCA TGATCCACGA GTGCCTAGGG ATCTTAGCAA CATCAATTGT
    GAAGAGCTCG GTCCTTTGCC TCCTGGATGG GAGATCCGCA ATACCGCAAC AGGAAGAGTT
    TATTTCGTTG ACCATAACAA CAGAACAACA CAATTTACAG ATCCCCGGCT CTCTGCTAAC
    TTGCATTTAG TTTTAAATCG TCAGAACCAG TTGAAAGACC AACAGCAACA GCAAGTGGTG
    CCATTGTGTC CCGATGACAC TGAGTGTCTG ACAGTGCCAA GATACAAGCG AGATTTGGTT
    CAAAAACTAA AAATCTTGCG GCAAGAACTT TCCCAACAGC AGCCTCAAGC TGGCCACTGC
    CGTATTGAGG TGTCTAGGGA AGAGATTTTT GAGGAATCAT ATAGACAGGT CATGAAAATG
    AGGCCAAAAG ATCTATGGAA GCGATTAATG ATAAAATTTC GTGGAGAAGA AGGTCTTGAC
    TATGGAGGAG TTGCCAGGGA ATGGTTGTAT CTCCTGTCAC ATGAAATGTT GAATCCTTAC
    TATGGTCTCT TCCAGTATTC TAGAGATGAT ATCTACACAT TGCAGATCAA TCCTGATTCT
    GCAGTTAATC CGGAACATTT ATCCTATTTC CACTTTGTTG GACGGATAAT GGGAATGGCT
    GTATTCCATG GACATTATAT TGACGGTGGC TTCACATTAC CTTTTTATAA ACAGTTGCTT
    GGGAAGTCAA TTACTTTGGA TGACATGGAG CTAGTTGACC CAGACCTTCA TAATAGTTTA
    GTGTGGATAC TTGAAAACGA TATCACAGGT GTTCTGGACC ATACCTTCTG TGTTGAGCAT
    AATGCATATG GTGAAATAAT CCAGCATGAA CTCAAACCAA ATGGCAAAAG TATCCCTGTT
    ACTGAAGAAA ATAAAAAAGA ATATGTCAGG CTTTATGTCA ACTGGAGATT TCTCCGAGGC
    ATCGAAGCTC AGTTCCTGGC GCTGCAGAAG GGCTTTAATG AAGTCATTCC CCAGCACCTG
    CTGAAGACAT TTGATGAGAA GGAGCTGGAG CTCATTATTT GTGGACTTGG CAAAATTGAT
    GTGAGTGACT GGAAGGTCAA CACCCGGTTA AAACACTGTA CCCCAGACAG CAATGTCGTC
    AAGTGGTTCT GGAAAGCGGT GGAGTTTTTT GATGAAGAGA GACGAGCACG GTTGCTCCAG
    TTTGTGACAG GGTCCTCTCG AGTGCCTCTG CAGGGCTTCA AAGCACTACA AGGTGCTGCA
    GGCCCACGGC TCTTTACCAT ACACCAGATT GATGCCTGCA CGAACAACTT GCCAAAAGCC
    CACACTTGCT TCAATCGAAT AGACATTCCA CCCTATGAAA GCTATGAGAA GCTCTATGAA
    AAGCTGCTAA CGGCCATCGA GGAGACATGT GGCTTTGCTG TGGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006533999.1
    CDS487..2694
    Translation

    Target ORF information:

    RefSeq Version XM_006533936.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus SMAD specific E3 ubiquitin protein ligase 2 (Smurf2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006533936.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCTAACC CCGGAGGCCG GAGGAACGGG CCCGTCAAGC TGCGCCTGAC AGGTCTTCCT 
    GATCCATTTG CTAAGGTGGT AGTTGATGGT TCTGGGCAGT GCCATTCTAC AGATACTGTG
    AAGAACACAC TTGATCCAAA GTGGAATCAG CATTATGACC TGTATATCGG AAAGTCTGAT
    TCAGTTACGA TCAGCGTATG GAATCATAAG AAGATCCATA AGAAGCAAGG TGCTGGATTT
    CTTGGTTGTG TTCGTCTTCT TTCCAATGCC ATCAACCGCC TCAAGGACAC TGGTTATCAG
    AGGTTGGATT TATGCAAACT GGGGCCAAAT GACAATGATA CAGTTCGAGG ACAAATTGTA
    GTAAGTCTTC AGTCCAGAGA CCGAATAGGC ACGGGAGGAC AAGTTGTGGA CTGTAGTCGT
    TTGTTTGACA ATGATTTGCC AGATGGTTGG GAAGAAAGGC GGACTGCCTC GGGAAGAATC
    CAGTATCTAA ACCACATCAC AAGAACTACA CAGTGGGAAC GCCCAACACG ACCGGCGTCA
    GAATATTCTA GTCCTGGCAG ACCCCTCAGC TGCTTTGTGG ATGAGAATAC TCCAATTACT
    GGAACAAATG GTGCAACGTG TGGACATTCT TCAGACCCCA GACTAGCAGA GAGAAGAGTC
    AGGTCCCAGC GACATAGAAA TTACATGAGC AGGACACACT TACACACTCC TCCAGACCTA
    CCGGAAGGCT ATGAACAAAG GACAACGCAG CAGGGTCAGG TATATTTCTT ACATACTCAG
    ACTGGTGTGA GCACATGGCA TGATCCACGA GTGCCTAGGG ATCTTAGCAA CATCAATTGT
    GAAGAGCTCG GTCCTTTGCC TCCTGGATGG GAGATCCGCA ATACCGCAAC AGGAAGAGTT
    TATTTCGTTG ACCATAACAA CAGAACAACA CAATTTACAG ATCCCCGGCT CTCTGCTAAC
    TTGCATTTAG TTTTAAATCG TCAGAACCAG TTGAAAGACC AACAGCAACA GCAAGTGGTG
    CCATTGTGTC CCGATGACAC TGAGTGTCTG ACAGTGCCAA GATACAAGCG AGATTTGGTT
    CAAAAACTAA AAATCTTGCG GCAAGAACTT TCCCAACAGC AGCCTCAAGC TGGCCACTGC
    CGTATTGAGG TGTCTAGGGA AGAGATTTTT GAGGAATCAT ATAGACAGGT CATGAAAATG
    AGGCCAAAAG ATCTATGGAA GCGATTAATG ATAAAATTTC GTGGAGAAGA AGGTCTTGAC
    TATGGAGGAG TTGCCAGGGA ATGGTTGTAT CTCCTGTCAC ATGAAATGTT GAATCCTTAC
    TATGGTCTCT TCCAGTATTC TAGAGATGAT ATCTACACAT TGCAGATCAA TCCTGATTCT
    GCAGTTAATC CGGAACATTT ATCCTATTTC CACTTTGTTG GACGGATAAT GGGAATGGCT
    GTATTCCATG GACATTATAT TGACGGTGGC TTCACATTAC CTTTTTATAA ACAGTTGCTT
    GGGAAGTCAA TTACTTTGGA TGACATGGAG CTAGTTGACC CAGACCTTCA TAATAGTTTA
    GTGTGGATAC TTGAAAACGA TATCACAGGT GTTCTGGACC ATACCTTCTG TGTTGAGCAT
    AATGCATATG GTGAAATAAT CCAGCATGAA CTCAAACCAA ATGGCAAAAG TATCCCTGTT
    ACTGAAGAAA ATAAAAAAGA ATATGTCAGG CTTTATGTCA ACTGGAGATT TCTCCGAGGC
    ATCGAAGCTC AGTTCCTGGC GCTGCAGAAG GGCTTTAATG AAGTCATTCC CCAGCACCTG
    CTGAAGACAT TTGATGAGAA GGAGCTGGAG CTCATTATTT GTGGACTTGG CAAAATTGAT
    GTGAGTGACT GGAAGGTCAA CACCCGGTTA AAACACTGTA CCCCAGACAG CAATGTCGTC
    AAGTGGTTCT GGAAAGCGGT GGAGTTTTTT GATGAAGAGA GACGAGCACG GTTGCTCCAG
    TTTGTGACAG GGTCCTCTCG AGTGCCTCTG CAGGGCTTCA AAGCACTACA AGGTGCTGCA
    GGCCCACGGC TCTTTACCAT ACACCAGATT GATGCCTGCA CGAACAACTT GCCAAAAGCC
    CACACTTGCT TCAATCGAAT AGACATTCCA CCCTATGAAA GCTATGAGAA GCTCTATGAA
    AAGCTGCTAA CGGCCATCGA GGAGACATGT GGCTTTGCTG TGGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.