Ethe1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ethe1
Entrez Gene ID	66071
Full Name	ethylmalonic encephalopathy 1
Synonyms	0610025L15Rik,Hsco
General protein information	Preferred Names ethylmalonic encephalopathy 1 Names persulfide dioxygenase ETHE1, mitochondrial ethylmalonic encephalopathy protein 1 homolog hepatoma subtracted clone one protein protein ETHE1, mitochondrial sulfur dioxygenase ETHE1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 A3

mRNA and Protein(s)

mRNA	Protein	Name
XM_030242879.1	XP_030098739.1	persulfide dioxygenase ETHE1, mitochondrial isoform X1
XM_030242880.1	XP_030098740.1	persulfide dioxygenase ETHE1, mitochondrial isoform X1
NM_001364014.1	NP_001350943.1	persulfide dioxygenase ETHE1, mitochondrial precursor
NM_023154.3	NP_075643.1	persulfide dioxygenase ETHE1, mitochondrial precursor
XM_017312221.1	XP_017167710.1	persulfide dioxygenase ETHE1, mitochondrial isoform X1
XM_030242881.1	XP_030098741.1	persulfide dioxygenase ETHE1, mitochondrial isoform X1
NM_023154.4	NP_075643.1	persulfide dioxygenase ETHE1, mitochondrial precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	ETHE1	NP_001029516.1
Mus musculus (house mouse)	Ethe1	NP_075643.1
Danio rerio (zebrafish)	ethe1	NP_998094.1
Drosophila melanogaster (fruit fly)	CG30022	NP_725047.2
Homo sapiens (human)	ETHE1	NP_055112.2
Arabidopsis thaliana (thale cress)	GLY3	NP_564636.2
Canis lupus familiaris (dog)	ETHE1	XP_855241.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003337	XP_314233.3
Oryza sativa (rice)	Os01g0667200	NP_001043807.1
Xenopus tropicalis (tropical clawed frog)	ethe1	NP_001005706.1
Pan troglodytes (chimpanzee)	ETHE1	XP_512716.2
	ETHE1	XP_001101587.1
Rattus norvegicus (Norway rat)	Ethe1	NP_001099704.1
Gallus gallus (chicken)	ETHE1	XP_424095.4
Caenorhabditis elegans (roundworm)	ethe-1	NP_501684.1

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Proteome adaptations in Ethe1-deficient mice indicate a role in lipid catabolism and cytoskeleton organization via post-translational protein modifications.
Hildebrandt TM, Di Meo I, Zeviani M, Viscomi C, Braun HP
Bioscience reports33(4)(2013 Jul)

Loss of ETHE1, a mitochondrial dioxygenase, causes fatal sulfide toxicity in ethylmalonic encephalopathy.
Tiranti V, Viscomi C, Hildebrandt T, Di Meo I, Mineri R, Tiveron C, Levitt MD, Prelle A, Fagiolari G, Rimoldi M, Zeviani M
Nature medicine15(2)200-5(2009 Feb)

Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria.
Mootha VK, Bunkenborg J, Olsen JV, Hjerrild M, Wisniewski JR, Stahl E, Bolouri MS, Ray HN, Sihag S, Kamal M, Patterson N, Lander ES, Mann M
Cell115(5)629-40(2003 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Ethe1 deficiency disrupts post translational amino acid modification and mitochondrial metabolic systems.
Title: Proteome adaptations in Ethe1-deficient mice indicate a role in lipid catabolism and cytoskeleton organization via post-translational protein modifications.

The following Ethe1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ethe1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu18800	NM_023154.4 Latest version!	Mus musculus ethylmalonic encephalopathy 1 (Ethe1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu18800	NM_001364014.1 Latest version!	Mus musculus ethylmalonic encephalopathy 1 (Ethe1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu18800	XM_030242880.1 Latest version!	Mus musculus ethylmalonic encephalopathy 1 (Ethe1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu18800	XM_030242879.1 Latest version!	Mus musculus ethylmalonic encephalopathy 1 (Ethe1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu18800	XM_017312221.1 Latest version!	Mus musculus ethylmalonic encephalopathy 1 (Ethe1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu18800	XM_030242881.1 Latest version!	Mus musculus ethylmalonic encephalopathy 1 (Ethe1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OMu18800	NM_023154.3	Mus musculus ethylmalonic encephalopathy 1 (Ethe1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ethe1 ( NM_001364014.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001350943.1 Mus musculus ethylmalonic encephalopathy 1 (Ethe1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu18800
Clone ID Related Accession (Same CDS sequence)	XM_030242879.1 , XM_030242880.1 , NM_001364014.1 , NM_023154.3 , XM_017312221.1 , XM_030242881.1 , NM_023154.4
Accession Version	NM_001364014.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 765bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Mus musculus(house mouse)
Product	persulfide dioxygenase ETHE1, mitochondrial precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK002666.1 and AI467554.1. On Jun 5, 2018 this sequence version replaced XM_017312221.1. ##Evidence-Data-START## Transcript exon combination :: BC010592.1, AK002666.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGAGCG CGGTCGTCAG GGTCGCCGGG CGGCGGCTGA GCCAGCAAAG CGCATCCGGA 
GCCCCGGTCC TCCTGCGGCA GATGTTTGAA CCCAAGAGCT GCACCTATAC CTACCTTCTG 
GGTGACCGGG AGTCAAGAGA GGCAGTTCTG ATCGACCCCG TTCTGGAGAC AGCGCATCGG 
GATGCTCAGT TGATTAAGGA GCTGGGGCTC AAGCTGTTGT ACGCTGTGAA CACTCACTGC 
CATGCTGACC ACATCACCGG CACGGGGGTT CTCCGGTCCC TGCTCCCGGG CTGCCAGTCT 
GTCATCTCCC GCCTCAGCGG AGCCCAGGCT GATTTGCATA TCGGGGAAGG TGATTCCATC 
CGCTTTGGAC GCTTTGCTTT GGAGACTCGA GCCAGCCCTG GCCACACTCC AGGCTGTGTC 
ACCTTTGTCC TGAACGACCA GAGCATGGCC TTCACTGGAG ATGCCCTGCT GATCCGAGGG 
TGTGGACGGA CAGACTTCCA ACAAGGCTGT GCTAAGACTT TGTACCACTC TGTGCACGAG 
AAGATCTTCA CACTTCCAGG CAACTGTCTA ATCTACCCGG CTCACGATTA CCACGGGCTC 
ACAGTTTCTA CTGTGGAGGA GGAACGGACT CTGAACCCAC GGCTTACCCT CAGCTGTGAG 
GAATTTATCA AGGTCATGGA CAACCTGAAC TTGCCCAAGC CACAGCAGAT AGACATTGCT 
GTTCCTGCAA ATATGCGCTG TGGGGTCCAG ACTCCACCCT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001350943.1
CDS	24..788
Translation	MASAVVRVAGRRLSQQSASGAPVLLRQMFEPKSCTYTYLLGDRESREAVLIDPVLETAHRDAQLIKELGLKLLYAVNTHCHADHITGTGVLRSLLPGCQSVISRLSGAQADLHIGEGDSIRFGRFALETRASPGHTPGCVTFVLNDQSMAFTGDALLIRGCGRTDFQQGCAKTLYHSVHEKIFTLPGNCLIYPAHDYHGLTVSTVEEERTLNPRLTLSCEEFIKVMDNLNLPKPQQIDIAVPANMRCGVQTPPS

Target ORF information:

RefSeq Version	NM_001364014.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ethylmalonic encephalopathy 1 (Ethe1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001364014.1

ORF Insert Sequence:

ATGGCGAGCG CGGTCGTCAG GGTCGCCGGG CGGCGGCTGA GCCAGCAAAG CGCATCCGGA 
GCCCCGGTCC TCCTGCGGCA GATGTTTGAA CCCAAGAGCT GCACCTATAC CTACCTTCTG 
GGTGACCGGG AGTCAAGAGA GGCAGTTCTG ATCGACCCCG TTCTGGAGAC AGCGCATCGG 
GATGCTCAGT TGATTAAGGA GCTGGGGCTC AAGCTGTTGT ACGCTGTGAA CACTCACTGC 
CATGCTGACC ACATCACCGG CACGGGGGTT CTCCGGTCCC TGCTCCCGGG CTGCCAGTCT 
GTCATCTCCC GCCTCAGCGG AGCCCAGGCT GATTTGCATA TCGGGGAAGG TGATTCCATC 
CGCTTTGGAC GCTTTGCTTT GGAGACTCGA GCCAGCCCTG GCCACACTCC AGGCTGTGTC 
ACCTTTGTCC TGAACGACCA GAGCATGGCC TTCACTGGAG ATGCCCTGCT GATCCGAGGG 
TGTGGACGGA CAGACTTCCA ACAAGGCTGT GCTAAGACTT TGTACCACTC TGTGCACGAG 
AAGATCTTCA CACTTCCAGG CAACTGTCTA ATCTACCCGG CTCACGATTA CCACGGGCTC 
ACAGTTTCTA CTGTGGAGGA GGAACGGACT CTGAACCCAC GGCTTACCCT CAGCTGTGAG 
GAATTTATCA AGGTCATGGA CAACCTGAAC TTGCCCAAGC CACAGCAGAT AGACATTGCT 
GTTCCTGCAA ATATGCGCTG TGGGGTCCAG ACTCCACCCT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ethe1 ( NM_023154.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_075643.1 Mus musculus ethylmalonic encephalopathy 1 (Ethe1), mRNA.

Price & Availability↑

CloneID	OMu18800
Clone ID Related Accession (Same CDS sequence)	XM_030242879.1 , XM_030242880.1 , NM_001364014.1 , NM_023154.3 , XM_017312221.1 , XM_030242881.1 , NM_023154.4
Accession Version	NM_023154.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 765bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-28
Organism	Mus musculus(house mouse)
Product	persulfide dioxygenase ETHE1, mitochondrial precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC094044.1. On May 1, 2005 this sequence version replaced NM_023154.2. ##Evidence-Data-START## Transcript exon combination :: BC094044.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164139 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGGCGAGCG CGGTCGTCAG GGTCGCCGGG CGGCGGCTGA GCCAGCAAAG CGCATCCGGA 
GCCCCGGTCC TCCTGCGGCA GATGTTTGAA CCCAAGAGCT GCACCTATAC CTACCTTCTG 
GGTGACCGGG AGTCAAGAGA GGCAGTTCTG ATCGACCCCG TTCTGGAGAC AGCGCATCGG 
GATGCTCAGT TGATTAAGGA GCTGGGGCTC AAGCTGTTGT ACGCTGTGAA CACTCACTGC 
CATGCTGACC ACATCACCGG CACGGGGGTT CTCCGGTCCC TGCTCCCGGG CTGCCAGTCT 
GTCATCTCCC GCCTCAGCGG AGCCCAGGCT GATTTGCATA TCGGGGAAGG TGATTCCATC 
CGCTTTGGAC GCTTTGCTTT GGAGACTCGA GCCAGCCCTG GCCACACTCC AGGCTGTGTC 
ACCTTTGTCC TGAACGACCA GAGCATGGCC TTCACTGGAG ATGCCCTGCT GATCCGAGGG 
TGTGGACGGA CAGACTTCCA ACAAGGCTGT GCTAAGACTT TGTACCACTC TGTGCACGAG 
AAGATCTTCA CACTTCCAGG CAACTGTCTA ATCTACCCGG CTCACGATTA CCACGGGCTC 
ACAGTTTCTA CTGTGGAGGA GGAACGGACT CTGAACCCAC GGCTTACCCT CAGCTGTGAG 
GAATTTATCA AGGTCATGGA CAACCTGAAC TTGCCCAAGC CACAGCAGAT AGACATTGCT 
GTTCCTGCAA ATATGCGCTG TGGGGTCCAG ACTCCACCCT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_075643.1
CDS	570..1334
Translation	MASAVVRVAGRRLSQQSASGAPVLLRQMFEPKSCTYTYLLGDRESREAVLIDPVLETAHRDAQLIKELGLKLLYAVNTHCHADHITGTGVLRSLLPGCQSVISRLSGAQADLHIGEGDSIRFGRFALETRASPGHTPGCVTFVLNDQSMAFTGDALLIRGCGRTDFQQGCAKTLYHSVHEKIFTLPGNCLIYPAHDYHGLTVSTVEEERTLNPRLTLSCEEFIKVMDNLNLPKPQQIDIAVPANMRCGVQTPPS

Target ORF information:

RefSeq Version	NM_023154.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ethylmalonic encephalopathy 1 (Ethe1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_023154.3

ORF Insert Sequence:

ATGGCGAGCG CGGTCGTCAG GGTCGCCGGG CGGCGGCTGA GCCAGCAAAG CGCATCCGGA 
GCCCCGGTCC TCCTGCGGCA GATGTTTGAA CCCAAGAGCT GCACCTATAC CTACCTTCTG 
GGTGACCGGG AGTCAAGAGA GGCAGTTCTG ATCGACCCCG TTCTGGAGAC AGCGCATCGG 
GATGCTCAGT TGATTAAGGA GCTGGGGCTC AAGCTGTTGT ACGCTGTGAA CACTCACTGC 
CATGCTGACC ACATCACCGG CACGGGGGTT CTCCGGTCCC TGCTCCCGGG CTGCCAGTCT 
GTCATCTCCC GCCTCAGCGG AGCCCAGGCT GATTTGCATA TCGGGGAAGG TGATTCCATC 
CGCTTTGGAC GCTTTGCTTT GGAGACTCGA GCCAGCCCTG GCCACACTCC AGGCTGTGTC 
ACCTTTGTCC TGAACGACCA GAGCATGGCC TTCACTGGAG ATGCCCTGCT GATCCGAGGG 
TGTGGACGGA CAGACTTCCA ACAAGGCTGT GCTAAGACTT TGTACCACTC TGTGCACGAG 
AAGATCTTCA CACTTCCAGG CAACTGTCTA ATCTACCCGG CTCACGATTA CCACGGGCTC 
ACAGTTTCTA CTGTGGAGGA GGAACGGACT CTGAACCCAC GGCTTACCCT CAGCTGTGAG 
GAATTTATCA AGGTCATGGA CAACCTGAAC TTGCCCAAGC CACAGCAGAT AGACATTGCT 
GTTCCTGCAA ATATGCGCTG TGGGGTCCAG ACTCCACCCT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ethe1 ( NM_023154.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_075643.1 Mus musculus ethylmalonic encephalopathy 1 (Ethe1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu18800
Clone ID Related Accession (Same CDS sequence)	XM_030242879.1 , XM_030242880.1 , NM_001364014.1 , NM_023154.3 , XM_017312221.1 , XM_030242881.1 , NM_023154.4
Accession Version	NM_023154.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 765bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Mus musculus(house mouse)
Product	persulfide dioxygenase ETHE1, mitochondrial precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC161166.7. On Jun 5, 2018 this sequence version replaced NM_023154.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC094044.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164139 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGAGCG CGGTCGTCAG GGTCGCCGGG CGGCGGCTGA GCCAGCAAAG CGCATCCGGA 
GCCCCGGTCC TCCTGCGGCA GATGTTTGAA CCCAAGAGCT GCACCTATAC CTACCTTCTG 
GGTGACCGGG AGTCAAGAGA GGCAGTTCTG ATCGACCCCG TTCTGGAGAC AGCGCATCGG 
GATGCTCAGT TGATTAAGGA GCTGGGGCTC AAGCTGTTGT ACGCTGTGAA CACTCACTGC 
CATGCTGACC ACATCACCGG CACGGGGGTT CTCCGGTCCC TGCTCCCGGG CTGCCAGTCT 
GTCATCTCCC GCCTCAGCGG AGCCCAGGCT GATTTGCATA TCGGGGAAGG TGATTCCATC 
CGCTTTGGAC GCTTTGCTTT GGAGACTCGA GCCAGCCCTG GCCACACTCC AGGCTGTGTC 
ACCTTTGTCC TGAACGACCA GAGCATGGCC TTCACTGGAG ATGCCCTGCT GATCCGAGGG 
TGTGGACGGA CAGACTTCCA ACAAGGCTGT GCTAAGACTT TGTACCACTC TGTGCACGAG 
AAGATCTTCA CACTTCCAGG CAACTGTCTA ATCTACCCGG CTCACGATTA CCACGGGCTC 
ACAGTTTCTA CTGTGGAGGA GGAACGGACT CTGAACCCAC GGCTTACCCT CAGCTGTGAG 
GAATTTATCA AGGTCATGGA CAACCTGAAC TTGCCCAAGC CACAGCAGAT AGACATTGCT 
GTTCCTGCAA ATATGCGCTG TGGGGTCCAG ACTCCACCCT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_075643.1
CDS	570..1334
Translation	MASAVVRVAGRRLSQQSASGAPVLLRQMFEPKSCTYTYLLGDRESREAVLIDPVLETAHRDAQLIKELGLKLLYAVNTHCHADHITGTGVLRSLLPGCQSVISRLSGAQADLHIGEGDSIRFGRFALETRASPGHTPGCVTFVLNDQSMAFTGDALLIRGCGRTDFQQGCAKTLYHSVHEKIFTLPGNCLIYPAHDYHGLTVSTVEEERTLNPRLTLSCEEFIKVMDNLNLPKPQQIDIAVPANMRCGVQTPPS

Target ORF information:

RefSeq Version	NM_023154.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus ethylmalonic encephalopathy 1 (Ethe1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_023154.4

ORF Insert Sequence:

ATGGCGAGCG CGGTCGTCAG GGTCGCCGGG CGGCGGCTGA GCCAGCAAAG CGCATCCGGA 
GCCCCGGTCC TCCTGCGGCA GATGTTTGAA CCCAAGAGCT GCACCTATAC CTACCTTCTG 
GGTGACCGGG AGTCAAGAGA GGCAGTTCTG ATCGACCCCG TTCTGGAGAC AGCGCATCGG 
GATGCTCAGT TGATTAAGGA GCTGGGGCTC AAGCTGTTGT ACGCTGTGAA CACTCACTGC 
CATGCTGACC ACATCACCGG CACGGGGGTT CTCCGGTCCC TGCTCCCGGG CTGCCAGTCT 
GTCATCTCCC GCCTCAGCGG AGCCCAGGCT GATTTGCATA TCGGGGAAGG TGATTCCATC 
CGCTTTGGAC GCTTTGCTTT GGAGACTCGA GCCAGCCCTG GCCACACTCC AGGCTGTGTC 
ACCTTTGTCC TGAACGACCA GAGCATGGCC TTCACTGGAG ATGCCCTGCT GATCCGAGGG 
TGTGGACGGA CAGACTTCCA ACAAGGCTGT GCTAAGACTT TGTACCACTC TGTGCACGAG 
AAGATCTTCA CACTTCCAGG CAACTGTCTA ATCTACCCGG CTCACGATTA CCACGGGCTC 
ACAGTTTCTA CTGTGGAGGA GGAACGGACT CTGAACCCAC GGCTTACCCT CAGCTGTGAG 
GAATTTATCA AGGTCATGGA CAACCTGAAC TTGCCCAAGC CACAGCAGAT AGACATTGCT 
GTTCCTGCAA ATATGCGCTG TGGGGTCCAG ACTCCACCCT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ethe1 ( XM_030242879.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030098739.1 Mus musculus ethylmalonic encephalopathy 1 (Ethe1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu18800
Clone ID Related Accession (Same CDS sequence)	XM_030242879.1 , XM_030242880.1 , NM_001364014.1 , NM_023154.3 , XM_017312221.1 , XM_030242881.1 , NM_023154.4
Accession Version	XM_030242879.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 765bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	persulfide dioxygenase ETHE1, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCG CGGTCGTCAG GGTCGCCGGG CGGCGGCTGA GCCAGCAAAG CGCATCCGGA 
GCCCCGGTCC TCCTGCGGCA GATGTTTGAA CCCAAGAGCT GCACCTATAC CTACCTTCTG 
GGTGACCGGG AGTCAAGAGA GGCAGTTCTG ATCGACCCCG TTCTGGAGAC AGCGCATCGG 
GATGCTCAGT TGATTAAGGA GCTGGGGCTC AAGCTGTTGT ACGCTGTGAA CACTCACTGC 
CATGCTGACC ACATCACCGG CACGGGGGTT CTCCGGTCCC TGCTCCCGGG CTGCCAGTCT 
GTCATCTCCC GCCTCAGCGG AGCCCAGGCT GATTTGCATA TCGGGGAAGG TGATTCCATC 
CGCTTTGGAC GCTTTGCTTT GGAGACTCGA GCCAGCCCTG GCCACACTCC AGGCTGTGTC 
ACCTTTGTCC TGAACGACCA GAGCATGGCC TTCACTGGAG ATGCCCTGCT GATCCGAGGG 
TGTGGACGGA CAGACTTCCA ACAAGGCTGT GCTAAGACTT TGTACCACTC TGTGCACGAG 
AAGATCTTCA CACTTCCAGG CAACTGTCTA ATCTACCCGG CTCACGATTA CCACGGGCTC 
ACAGTTTCTA CTGTGGAGGA GGAACGGACT CTGAACCCAC GGCTTACCCT CAGCTGTGAG 
GAATTTATCA AGGTCATGGA CAACCTGAAC TTGCCCAAGC CACAGCAGAT AGACATTGCT 
GTTCCTGCAA ATATGCGCTG TGGGGTCCAG ACTCCACCCT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030098739.1
CDS	636..1400
Translation	MASAVVRVAGRRLSQQSASGAPVLLRQMFEPKSCTYTYLLGDRESREAVLIDPVLETAHRDAQLIKELGLKLLYAVNTHCHADHITGTGVLRSLLPGCQSVISRLSGAQADLHIGEGDSIRFGRFALETRASPGHTPGCVTFVLNDQSMAFTGDALLIRGCGRTDFQQGCAKTLYHSVHEKIFTLPGNCLIYPAHDYHGLTVSTVEEERTLNPRLTLSCEEFIKVMDNLNLPKPQQIDIAVPANMRCGVQTPPS

Target ORF information:

RefSeq Version	XM_030242879.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ethylmalonic encephalopathy 1 (Ethe1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030242879.1

ORF Insert Sequence:

ATGGCGAGCG CGGTCGTCAG GGTCGCCGGG CGGCGGCTGA GCCAGCAAAG CGCATCCGGA 
GCCCCGGTCC TCCTGCGGCA GATGTTTGAA CCCAAGAGCT GCACCTATAC CTACCTTCTG 
GGTGACCGGG AGTCAAGAGA GGCAGTTCTG ATCGACCCCG TTCTGGAGAC AGCGCATCGG 
GATGCTCAGT TGATTAAGGA GCTGGGGCTC AAGCTGTTGT ACGCTGTGAA CACTCACTGC 
CATGCTGACC ACATCACCGG CACGGGGGTT CTCCGGTCCC TGCTCCCGGG CTGCCAGTCT 
GTCATCTCCC GCCTCAGCGG AGCCCAGGCT GATTTGCATA TCGGGGAAGG TGATTCCATC 
CGCTTTGGAC GCTTTGCTTT GGAGACTCGA GCCAGCCCTG GCCACACTCC AGGCTGTGTC 
ACCTTTGTCC TGAACGACCA GAGCATGGCC TTCACTGGAG ATGCCCTGCT GATCCGAGGG 
TGTGGACGGA CAGACTTCCA ACAAGGCTGT GCTAAGACTT TGTACCACTC TGTGCACGAG 
AAGATCTTCA CACTTCCAGG CAACTGTCTA ATCTACCCGG CTCACGATTA CCACGGGCTC 
ACAGTTTCTA CTGTGGAGGA GGAACGGACT CTGAACCCAC GGCTTACCCT CAGCTGTGAG 
GAATTTATCA AGGTCATGGA CAACCTGAAC TTGCCCAAGC CACAGCAGAT AGACATTGCT 
GTTCCTGCAA ATATGCGCTG TGGGGTCCAG ACTCCACCCT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ethe1 ( XM_030242880.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030098740.1 Mus musculus ethylmalonic encephalopathy 1 (Ethe1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu18800
Clone ID Related Accession (Same CDS sequence)	XM_030242879.1 , XM_030242880.1 , NM_001364014.1 , NM_023154.3 , XM_017312221.1 , XM_030242881.1 , NM_023154.4
Accession Version	XM_030242880.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 765bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	persulfide dioxygenase ETHE1, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCG CGGTCGTCAG GGTCGCCGGG CGGCGGCTGA GCCAGCAAAG CGCATCCGGA 
GCCCCGGTCC TCCTGCGGCA GATGTTTGAA CCCAAGAGCT GCACCTATAC CTACCTTCTG 
GGTGACCGGG AGTCAAGAGA GGCAGTTCTG ATCGACCCCG TTCTGGAGAC AGCGCATCGG 
GATGCTCAGT TGATTAAGGA GCTGGGGCTC AAGCTGTTGT ACGCTGTGAA CACTCACTGC 
CATGCTGACC ACATCACCGG CACGGGGGTT CTCCGGTCCC TGCTCCCGGG CTGCCAGTCT 
GTCATCTCCC GCCTCAGCGG AGCCCAGGCT GATTTGCATA TCGGGGAAGG TGATTCCATC 
CGCTTTGGAC GCTTTGCTTT GGAGACTCGA GCCAGCCCTG GCCACACTCC AGGCTGTGTC 
ACCTTTGTCC TGAACGACCA GAGCATGGCC TTCACTGGAG ATGCCCTGCT GATCCGAGGG 
TGTGGACGGA CAGACTTCCA ACAAGGCTGT GCTAAGACTT TGTACCACTC TGTGCACGAG 
AAGATCTTCA CACTTCCAGG CAACTGTCTA ATCTACCCGG CTCACGATTA CCACGGGCTC 
ACAGTTTCTA CTGTGGAGGA GGAACGGACT CTGAACCCAC GGCTTACCCT CAGCTGTGAG 
GAATTTATCA AGGTCATGGA CAACCTGAAC TTGCCCAAGC CACAGCAGAT AGACATTGCT 
GTTCCTGCAA ATATGCGCTG TGGGGTCCAG ACTCCACCCT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030098740.1
CDS	610..1374
Translation	MASAVVRVAGRRLSQQSASGAPVLLRQMFEPKSCTYTYLLGDRESREAVLIDPVLETAHRDAQLIKELGLKLLYAVNTHCHADHITGTGVLRSLLPGCQSVISRLSGAQADLHIGEGDSIRFGRFALETRASPGHTPGCVTFVLNDQSMAFTGDALLIRGCGRTDFQQGCAKTLYHSVHEKIFTLPGNCLIYPAHDYHGLTVSTVEEERTLNPRLTLSCEEFIKVMDNLNLPKPQQIDIAVPANMRCGVQTPPS

Target ORF information:

RefSeq Version	XM_030242880.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ethylmalonic encephalopathy 1 (Ethe1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030242880.1

ORF Insert Sequence:

ATGGCGAGCG CGGTCGTCAG GGTCGCCGGG CGGCGGCTGA GCCAGCAAAG CGCATCCGGA 
GCCCCGGTCC TCCTGCGGCA GATGTTTGAA CCCAAGAGCT GCACCTATAC CTACCTTCTG 
GGTGACCGGG AGTCAAGAGA GGCAGTTCTG ATCGACCCCG TTCTGGAGAC AGCGCATCGG 
GATGCTCAGT TGATTAAGGA GCTGGGGCTC AAGCTGTTGT ACGCTGTGAA CACTCACTGC 
CATGCTGACC ACATCACCGG CACGGGGGTT CTCCGGTCCC TGCTCCCGGG CTGCCAGTCT 
GTCATCTCCC GCCTCAGCGG AGCCCAGGCT GATTTGCATA TCGGGGAAGG TGATTCCATC 
CGCTTTGGAC GCTTTGCTTT GGAGACTCGA GCCAGCCCTG GCCACACTCC AGGCTGTGTC 
ACCTTTGTCC TGAACGACCA GAGCATGGCC TTCACTGGAG ATGCCCTGCT GATCCGAGGG 
TGTGGACGGA CAGACTTCCA ACAAGGCTGT GCTAAGACTT TGTACCACTC TGTGCACGAG 
AAGATCTTCA CACTTCCAGG CAACTGTCTA ATCTACCCGG CTCACGATTA CCACGGGCTC 
ACAGTTTCTA CTGTGGAGGA GGAACGGACT CTGAACCCAC GGCTTACCCT CAGCTGTGAG 
GAATTTATCA AGGTCATGGA CAACCTGAAC TTGCCCAAGC CACAGCAGAT AGACATTGCT 
GTTCCTGCAA ATATGCGCTG TGGGGTCCAG ACTCCACCCT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ethe1 ( XM_017312221.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017167710.1 Mus musculus ethylmalonic encephalopathy 1 (Ethe1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu18800
Clone ID Related Accession (Same CDS sequence)	XM_030242879.1 , XM_030242880.1 , NM_001364014.1 , NM_023154.3 , XM_017312221.1 , XM_030242881.1 , NM_023154.4
Accession Version	XM_017312221.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 765bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	persulfide dioxygenase ETHE1, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCG CGGTCGTCAG GGTCGCCGGG CGGCGGCTGA GCCAGCAAAG CGCATCCGGA 
GCCCCGGTCC TCCTGCGGCA GATGTTTGAA CCCAAGAGCT GCACCTATAC CTACCTTCTG 
GGTGACCGGG AGTCAAGAGA GGCAGTTCTG ATCGACCCCG TTCTGGAGAC AGCGCATCGG 
GATGCTCAGT TGATTAAGGA GCTGGGGCTC AAGCTGTTGT ACGCTGTGAA CACTCACTGC 
CATGCTGACC ACATCACCGG CACGGGGGTT CTCCGGTCCC TGCTCCCGGG CTGCCAGTCT 
GTCATCTCCC GCCTCAGCGG AGCCCAGGCT GATTTGCATA TCGGGGAAGG TGATTCCATC 
CGCTTTGGAC GCTTTGCTTT GGAGACTCGA GCCAGCCCTG GCCACACTCC AGGCTGTGTC 
ACCTTTGTCC TGAACGACCA GAGCATGGCC TTCACTGGAG ATGCCCTGCT GATCCGAGGG 
TGTGGACGGA CAGACTTCCA ACAAGGCTGT GCTAAGACTT TGTACCACTC TGTGCACGAG 
AAGATCTTCA CACTTCCAGG CAACTGTCTA ATCTACCCGG CTCACGATTA CCACGGGCTC 
ACAGTTTCTA CTGTGGAGGA GGAACGGACT CTGAACCCAC GGCTTACCCT CAGCTGTGAG 
GAATTTATCA AGGTCATGGA CAACCTGAAC TTGCCCAAGC CACAGCAGAT AGACATTGCT 
GTTCCTGCAA ATATGCGCTG TGGGGTCCAG ACTCCACCCT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017167710.1
CDS	598..1362
Translation	MASAVVRVAGRRLSQQSASGAPVLLRQMFEPKSCTYTYLLGDRESREAVLIDPVLETAHRDAQLIKELGLKLLYAVNTHCHADHITGTGVLRSLLPGCQSVISRLSGAQADLHIGEGDSIRFGRFALETRASPGHTPGCVTFVLNDQSMAFTGDALLIRGCGRTDFQQGCAKTLYHSVHEKIFTLPGNCLIYPAHDYHGLTVSTVEEERTLNPRLTLSCEEFIKVMDNLNLPKPQQIDIAVPANMRCGVQTPPS

Target ORF information:

RefSeq Version	XM_017312221.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ethylmalonic encephalopathy 1 (Ethe1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312221.1

ORF Insert Sequence:

ATGGCGAGCG CGGTCGTCAG GGTCGCCGGG CGGCGGCTGA GCCAGCAAAG CGCATCCGGA 
GCCCCGGTCC TCCTGCGGCA GATGTTTGAA CCCAAGAGCT GCACCTATAC CTACCTTCTG 
GGTGACCGGG AGTCAAGAGA GGCAGTTCTG ATCGACCCCG TTCTGGAGAC AGCGCATCGG 
GATGCTCAGT TGATTAAGGA GCTGGGGCTC AAGCTGTTGT ACGCTGTGAA CACTCACTGC 
CATGCTGACC ACATCACCGG CACGGGGGTT CTCCGGTCCC TGCTCCCGGG CTGCCAGTCT 
GTCATCTCCC GCCTCAGCGG AGCCCAGGCT GATTTGCATA TCGGGGAAGG TGATTCCATC 
CGCTTTGGAC GCTTTGCTTT GGAGACTCGA GCCAGCCCTG GCCACACTCC AGGCTGTGTC 
ACCTTTGTCC TGAACGACCA GAGCATGGCC TTCACTGGAG ATGCCCTGCT GATCCGAGGG 
TGTGGACGGA CAGACTTCCA ACAAGGCTGT GCTAAGACTT TGTACCACTC TGTGCACGAG 
AAGATCTTCA CACTTCCAGG CAACTGTCTA ATCTACCCGG CTCACGATTA CCACGGGCTC 
ACAGTTTCTA CTGTGGAGGA GGAACGGACT CTGAACCCAC GGCTTACCCT CAGCTGTGAG 
GAATTTATCA AGGTCATGGA CAACCTGAAC TTGCCCAAGC CACAGCAGAT AGACATTGCT 
GTTCCTGCAA ATATGCGCTG TGGGGTCCAG ACTCCACCCT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ethe1 ( XM_030242881.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030098741.1 Mus musculus ethylmalonic encephalopathy 1 (Ethe1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu18800
Clone ID Related Accession (Same CDS sequence)	XM_030242879.1 , XM_030242880.1 , NM_001364014.1 , NM_023154.3 , XM_017312221.1 , XM_030242881.1 , NM_023154.4
Accession Version	XM_030242881.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 765bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	persulfide dioxygenase ETHE1, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCG CGGTCGTCAG GGTCGCCGGG CGGCGGCTGA GCCAGCAAAG CGCATCCGGA 
GCCCCGGTCC TCCTGCGGCA GATGTTTGAA CCCAAGAGCT GCACCTATAC CTACCTTCTG 
GGTGACCGGG AGTCAAGAGA GGCAGTTCTG ATCGACCCCG TTCTGGAGAC AGCGCATCGG 
GATGCTCAGT TGATTAAGGA GCTGGGGCTC AAGCTGTTGT ACGCTGTGAA CACTCACTGC 
CATGCTGACC ACATCACCGG CACGGGGGTT CTCCGGTCCC TGCTCCCGGG CTGCCAGTCT 
GTCATCTCCC GCCTCAGCGG AGCCCAGGCT GATTTGCATA TCGGGGAAGG TGATTCCATC 
CGCTTTGGAC GCTTTGCTTT GGAGACTCGA GCCAGCCCTG GCCACACTCC AGGCTGTGTC 
ACCTTTGTCC TGAACGACCA GAGCATGGCC TTCACTGGAG ATGCCCTGCT GATCCGAGGG 
TGTGGACGGA CAGACTTCCA ACAAGGCTGT GCTAAGACTT TGTACCACTC TGTGCACGAG 
AAGATCTTCA CACTTCCAGG CAACTGTCTA ATCTACCCGG CTCACGATTA CCACGGGCTC 
ACAGTTTCTA CTGTGGAGGA GGAACGGACT CTGAACCCAC GGCTTACCCT CAGCTGTGAG 
GAATTTATCA AGGTCATGGA CAACCTGAAC TTGCCCAAGC CACAGCAGAT AGACATTGCT 
GTTCCTGCAA ATATGCGCTG TGGGGTCCAG ACTCCACCCT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030098741.1
CDS	536..1300
Translation	MASAVVRVAGRRLSQQSASGAPVLLRQMFEPKSCTYTYLLGDRESREAVLIDPVLETAHRDAQLIKELGLKLLYAVNTHCHADHITGTGVLRSLLPGCQSVISRLSGAQADLHIGEGDSIRFGRFALETRASPGHTPGCVTFVLNDQSMAFTGDALLIRGCGRTDFQQGCAKTLYHSVHEKIFTLPGNCLIYPAHDYHGLTVSTVEEERTLNPRLTLSCEEFIKVMDNLNLPKPQQIDIAVPANMRCGVQTPPS

Target ORF information:

RefSeq Version	XM_030242881.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ethylmalonic encephalopathy 1 (Ethe1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030242881.1

ORF Insert Sequence:

ATGGCGAGCG CGGTCGTCAG GGTCGCCGGG CGGCGGCTGA GCCAGCAAAG CGCATCCGGA 
GCCCCGGTCC TCCTGCGGCA GATGTTTGAA CCCAAGAGCT GCACCTATAC CTACCTTCTG 
GGTGACCGGG AGTCAAGAGA GGCAGTTCTG ATCGACCCCG TTCTGGAGAC AGCGCATCGG 
GATGCTCAGT TGATTAAGGA GCTGGGGCTC AAGCTGTTGT ACGCTGTGAA CACTCACTGC 
CATGCTGACC ACATCACCGG CACGGGGGTT CTCCGGTCCC TGCTCCCGGG CTGCCAGTCT 
GTCATCTCCC GCCTCAGCGG AGCCCAGGCT GATTTGCATA TCGGGGAAGG TGATTCCATC 
CGCTTTGGAC GCTTTGCTTT GGAGACTCGA GCCAGCCCTG GCCACACTCC AGGCTGTGTC 
ACCTTTGTCC TGAACGACCA GAGCATGGCC TTCACTGGAG ATGCCCTGCT GATCCGAGGG 
TGTGGACGGA CAGACTTCCA ACAAGGCTGT GCTAAGACTT TGTACCACTC TGTGCACGAG 
AAGATCTTCA CACTTCCAGG CAACTGTCTA ATCTACCCGG CTCACGATTA CCACGGGCTC 
ACAGTTTCTA CTGTGGAGGA GGAACGGACT CTGAACCCAC GGCTTACCCT CAGCTGTGAG 
GAATTTATCA AGGTCATGGA CAACCTGAAC TTGCCCAAGC CACAGCAGAT AGACATTGCT 
GTTCCTGCAA ATATGCGCTG TGGGGTCCAG ACTCCACCCT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Proteome adaptations in Ethe1-deficient mice indicate a role in lipid catabolism and cytoskeleton organization via post-translational protein modifications.
Bioscience reports33(4)(2013 Jul)
Hildebrandt TM,Di Meo I,Zeviani M,Viscomi C,Braun HP

Loss of ETHE1, a mitochondrial dioxygenase, causes fatal sulfide toxicity in ethylmalonic encephalopathy.
Nature medicine15(2)200-5(2009 Feb)
Tiranti V,Viscomi C,Hildebrandt T,Di Meo I,Mineri R,Tiveron C,Levitt MD,Prelle A,Fagiolari G,Rimoldi M,Zeviani M

Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria.
Cell115(5)629-40(2003 Nov)
Mootha VK,Bunkenborg J,Olsen JV,Hjerrild M,Wisniewski JR,Stahl E,Bolouri MS,Ray HN,Sihag S,Kamal M,Patterson N,Lander ES,Mann M

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Ethe1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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