Scel cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Scel
Entrez Gene ID	64929
Full Name	sciellin
Synonyms	9230114I02Rik
General protein information	Preferred Names sciellin Names sciellin
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 14 Map Location: 14\|14 E2.3

mRNA and Protein(s)

mRNA	Protein	Name
XM_006519355.2	XP_006519418.1	sciellin isoform X1
XM_006519355.4	XP_006519418.1	sciellin isoform X1
XM_006519355.3	XP_006519418.1	sciellin isoform X1
NM_022886.2	NP_075024.2	sciellin
XM_006519356.3	XP_006519419.1	sciellin isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	SCEL	NP_659001.2
Canis lupus familiaris (dog)	SCEL	XP_005634056.1
Gallus gallus (chicken)	SCEL	XP_004938676.1
Xenopus tropicalis (tropical clawed frog)	scel	XP_002931973.1
	SCEL	XP_001087679.1
Mus musculus (house mouse)	Scel	NP_075024.2
Rattus norvegicus (Norway rat)	Scel	NP_001101858.1
Pan troglodytes (chimpanzee)	SCEL	XP_001141124.1
Bos taurus (cattle)	SCEL	NP_001095802.1

Gene Ontology
Bibliography

Related articles in PubMed

Targeted deletion of the sciellin gene resulted in normal development and maturation.
Baden HP, Champliaud MF, Sundberg JP, Viel A
Genesis (New York, N.Y. : 2000)42(4)219-28(2005 Aug)

Evidence for a conserved function in synapse formation reveals Phr1 as a candidate gene for respiratory failure in newborn mice.
Burgess RW, Peterson KA, Johnson MJ, Roix JJ, Welsh IC, O'Brien TP
Molecular and cellular biology24(3)1096-105(2004 Feb)

Targeting a complex transcriptome: the construction of the mouse full-length cDNA encyclopedia.
Carninci P, Waki K, Shiraki T, Konno H, Shibata K, Itoh M, Aizawa K, Arakawa T, Ishii Y, Sasaki D, Bono H, Kondo S, Sugahara Y, Saito R, Osato N, Fukuda S, Sato K, Watahiki A, Hirozane-Kishikawa T, Nakamura M, Shibata Y, Yasunishi A, Kikuchi N, Yoshiki A, Kusakabe M, Gustincich S, Beisel K, Pavan W, Aidinis V, Nakagawara A, Held WA, Iwata H, Kono T, Nakauchi H, Lyons P, Wells C, Hume DA, Fagiolini M, Hensch TK, Brinkmeier M, Camper S, Hirota J, Mombaerts P, Muramatsu M, Okazaki Y, Kawai J, Hayashizaki Y
Genome research13(6B)1273-89(2003 Jun)

Functional and comparative genomic analysis of the piebald deletion region of mouse chromosome 14.
Peterson KA, King BL, Hagge-Greenberg A, Roix JJ, Bult CJ, O'Brien TP
Genomics80(2)172-84(2002 Aug)

Gene characterization of sciellin (SCEL) and protein localization in vertebrate epithelia displaying barrier properties.
Champliaud MF, Baden HP, Koch M, Jin W, Burgeson RE, Viel A
Genomics70(2)264-8(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We conclude that sciellin null mice had no structural anomalies and the transgenic mice did not act as a dominant-negative mutation.
Title: Targeted deletion of the sciellin gene resulted in normal development and maturation.

The following Scel gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Scel cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu50224	XM_006519356.3 Latest version!	Mus musculus sciellin (Scel), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu15713	NM_022886.2 Latest version!	Mus musculus sciellin (Scel), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OMu15713	XM_006519355.4 Latest version!	Mus musculus sciellin (Scel), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu15713	XM_006519355.2	Mus musculus sciellin (Scel), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OMu15713	XM_006519355.3	Mus musculus sciellin (Scel), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Scel ( XM_006519356.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006519419.1 Mus musculus sciellin (Scel), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu50224
Clone ID Related Accession (Same CDS sequence)	XM_006519356.3
Accession Version	XM_006519356.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	sciellin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006519356.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAATT TTTCCTCTAG AAAAAAGTCT CCAACTGGAA ATGATCTGAA GAGCGCCCGG 
GGAGCTGAGC TGAGACAACA GGGTTTTCAG GACGTGAACA AAAGAAGGGC TTTCTTACAG 
GACAACAGCT GGATAAAGAA GCCCCCAGAG GAAGAACAAG ACGGAAATTA TGGCAGGGTG 
GTGCTCAACC GGCACAACTC CCATGATGCC TTGGACAGGA AATTAATAGA GAGAGATGAG 
CCAAAAGCCA CAATTAGCCG GTACAGATCT GAGGACATGC TGGACAGAAC ACTGTCCTCA 
TTTAGAACGC CACAATCAAC CAAGACGCCA GCTGTCAGCT CCTTCAATGC AAACACTACC 
GCCACAGCTT CAACCCCCGC TACCACTCCT GTGAAGAAAA AGAGACAGTC CTGGTTCCCA 
CCACCCCCTC CTGGTCACAA TGCCTCTCCA AGCACAGCAG CCAGCAGAAG GGACCCAGCT 
CTGCACCCTC CACTCCCCCC AAAGCCCTGT TCTCCCATTG CTTCTCCTAA ACCATTGGGG 
CGGACTAATA GGCAGATACA TGCAGCTACA GCGGGGGCAT GTGGAGAAAC AGAACGACAT 
GCTGAAAGAA ATATAAGGAC TGAGGATCTC GATGACATCA TCAGGGTGGC GGCTGCACTT 
CAGAAAACTG ACAAGGGTGA AGAATTGGAT AATCTCATCA GAATGAACAA AAGCTTGAAC 
AGAAACCAAG GTCTCGATGG CCTCTTCCGA GCAAACCTGA AGGCACAGCA ATTGGACAAA 
AGAGCCCAGA GTCTTGAAAG CCTGATCTAT ATGAACACAC AGACGGACAG AGATGGCAAA 
GGAAATCAAG CCTTCGGGAG CCTTAAGAAA ATCAACCAAA GGGCTGACCC CGACAGAAGA 
AGCCAAGATC TCAGATCAGT TATCAGAACA CATGCCACGG CTGAGAGAAT CGGCAGAAGA 
AAACAAGACC TCGATGGACT GATTAAAGTG AATCCTGACA CAAATAAAAA TATGAAGAGG 
GGCAAGAGCC TTGACAATCT TATCAAAGTC ACCCCGGAAG TAAACAGAAG CAACAAAGGG 
GGTCCAAGTC TTGACAATTT CACCAAAGGA GTTCCTGCAA GGAGCAGAGC TAACCAAAGG 
GACCAAGACC TGGACAGTCT TATCAAGGTG ACCCCCTCAG CAAACAGAAG CCAGCACAGT 
CTTGATGAAC TTATTAATAC AAGCCCCCAA ACTATCAAAA CTACTGCTAG ACACCAAGAT 
CTCGATAAGT TCATCAAAGT AAATCCTGAT GTTCTCACAA ACAACCAAAG AAACCATGAT 
GTAGACAGCA CCATCAGAGG GAATCCTACA GGGACCAGAT GCGAGCAAAG CGAAGAGTTG 
GACAATCTTA TCAAAGTGAA GCCTTCTGCT CTCAGAAACA CAAATGGAGG CCAGGACCTA 
GAAAGCTTAA CTGAAGTAAA TTCTCATGTG GCTGAGAAGA ATGGGAGGAT AGATGGCCAA 
GCCAATGGAT TGACTAACAG TCTGTTCAAG GAGTCCACAA GAGCCTCGGT CTATTCTTAT 
GAAGCCAGAA ACAGTCTCAG CTCCAATTCT GGAAACAAGA ATGGTGGACC CAAGGACACT 
GTTGTTTATA CAAGGACATA TGTGGAGAAC AGTAAATCAC CAAAGGATGG TTATCAGGAA 
AATATCTCAG GAAAATATAT ACAAACCGTT TATTCAACTT CAGATAGGTC TGTCATTGAA 
AGAGACATGT GCACTTACTG CCGCAAGCCC TTGGGGGTGG AAACCAAGAT GATTTTAGAT 
GAATTACAGA TCTGTTGCCA TTCCACCTGC TTTAAGTGTG AAATATGCAA ACGGCCTCTG 
GAAAATTTAC AGGCAGGAGA CAGTATTTGG ATCTATAGGC AGACGATACA CTGTGAGCCC 
TGCTACTCTA AAGTGATGGC AAAATGGATT CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006519419.1
CDS	428..2383
Translation	MSNFSSRKKSPTGNDLKSARGAELRQQGFQDVNKRRAFLQDNSWIKKPPEEEQDGNYGRVVLNRHNSHDALDRKLIERDEPKATISRYRSEDMLDRTLSSFRTPQSTKTPAVSSFNANTTATASTPATTPVKKKRQSWFPPPPPGHNASPSTAASRRDPALHPPLPPKPCSPIASPKPLGRTNRQIHAATAGACGETERHAERNIRTEDLDDIIRVAAALQKTDKGEELDNLIRMNKSLNRNQGLDGLFRANLKAQQLDKRAQSLESLIYMNTQTDRDGKGNQAFGSLKKINQRADPDRRSQDLRSVIRTHATAERIGRRKQDLDGLIKVNPDTNKNMKRGKSLDNLIKVTPEVNRSNKGGPSLDNFTKGVPARSRANQRDQDLDSLIKVTPSANRSQHSLDELINTSPQTIKTTARHQDLDKFIKVNPDVLTNNQRNHDVDSTIRGNPTGTRCEQSEELDNLIKVKPSALRNTNGGQDLESLTEVNSHVAEKNGRIDGQANGLTNSLFKESTRASVYSYEARNSLSSNSGNKNGGPKDTVVYTRTYVENSKSPKDGYQENISGKYIQTVYSTSDRSVIERDMCTYCRKPLGVETKMILDELQICCHSTCFKCEICKRPLENLQAGDSIWIYRQTIHCEPCYSKVMAKWIQ

Target ORF information:

RefSeq Version	XM_006519356.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus sciellin (Scel), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006519356.3

ORF Insert Sequence:

ATGTCAAATT TTTCCTCTAG AAAAAAGTCT CCAACTGGAA ATGATCTGAA GAGCGCCCGG 
GGAGCTGAGC TGAGACAACA GGGTTTTCAG GACGTGAACA AAAGAAGGGC TTTCTTACAG 
GACAACAGCT GGATAAAGAA GCCCCCAGAG GAAGAACAAG ACGGAAATTA TGGCAGGGTG 
GTGCTCAACC GGCACAACTC CCATGATGCC TTGGACAGGA AATTAATAGA GAGAGATGAG 
CCAAAAGCCA CAATTAGCCG GTACAGATCT GAGGACATGC TGGACAGAAC ACTGTCCTCA 
TTTAGAACGC CACAATCAAC CAAGACGCCA GCTGTCAGCT CCTTCAATGC AAACACTACC 
GCCACAGCTT CAACCCCCGC TACCACTCCT GTGAAGAAAA AGAGACAGTC CTGGTTCCCA 
CCACCCCCTC CTGGTCACAA TGCCTCTCCA AGCACAGCAG CCAGCAGAAG GGACCCAGCT 
CTGCACCCTC CACTCCCCCC AAAGCCCTGT TCTCCCATTG CTTCTCCTAA ACCATTGGGG 
CGGACTAATA GGCAGATACA TGCAGCTACA GCGGGGGCAT GTGGAGAAAC AGAACGACAT 
GCTGAAAGAA ATATAAGGAC TGAGGATCTC GATGACATCA TCAGGGTGGC GGCTGCACTT 
CAGAAAACTG ACAAGGGTGA AGAATTGGAT AATCTCATCA GAATGAACAA AAGCTTGAAC 
AGAAACCAAG GTCTCGATGG CCTCTTCCGA GCAAACCTGA AGGCACAGCA ATTGGACAAA 
AGAGCCCAGA GTCTTGAAAG CCTGATCTAT ATGAACACAC AGACGGACAG AGATGGCAAA 
GGAAATCAAG CCTTCGGGAG CCTTAAGAAA ATCAACCAAA GGGCTGACCC CGACAGAAGA 
AGCCAAGATC TCAGATCAGT TATCAGAACA CATGCCACGG CTGAGAGAAT CGGCAGAAGA 
AAACAAGACC TCGATGGACT GATTAAAGTG AATCCTGACA CAAATAAAAA TATGAAGAGG 
GGCAAGAGCC TTGACAATCT TATCAAAGTC ACCCCGGAAG TAAACAGAAG CAACAAAGGG 
GGTCCAAGTC TTGACAATTT CACCAAAGGA GTTCCTGCAA GGAGCAGAGC TAACCAAAGG 
GACCAAGACC TGGACAGTCT TATCAAGGTG ACCCCCTCAG CAAACAGAAG CCAGCACAGT 
CTTGATGAAC TTATTAATAC AAGCCCCCAA ACTATCAAAA CTACTGCTAG ACACCAAGAT 
CTCGATAAGT TCATCAAAGT AAATCCTGAT GTTCTCACAA ACAACCAAAG AAACCATGAT 
GTAGACAGCA CCATCAGAGG GAATCCTACA GGGACCAGAT GCGAGCAAAG CGAAGAGTTG 
GACAATCTTA TCAAAGTGAA GCCTTCTGCT CTCAGAAACA CAAATGGAGG CCAGGACCTA 
GAAAGCTTAA CTGAAGTAAA TTCTCATGTG GCTGAGAAGA ATGGGAGGAT AGATGGCCAA 
GCCAATGGAT TGACTAACAG TCTGTTCAAG GAGTCCACAA GAGCCTCGGT CTATTCTTAT 
GAAGCCAGAA ACAGTCTCAG CTCCAATTCT GGAAACAAGA ATGGTGGACC CAAGGACACT 
GTTGTTTATA CAAGGACATA TGTGGAGAAC AGTAAATCAC CAAAGGATGG TTATCAGGAA 
AATATCTCAG GAAAATATAT ACAAACCGTT TATTCAACTT CAGATAGGTC TGTCATTGAA 
AGAGACATGT GCACTTACTG CCGCAAGCCC TTGGGGGTGG AAACCAAGAT GATTTTAGAT 
GAATTACAGA TCTGTTGCCA TTCCACCTGC TTTAAGTGTG AAATATGCAA ACGGCCTCTG 
GAAAATTTAC AGGCAGGAGA CAGTATTTGG ATCTATAGGC AGACGATACA CTGTGAGCCC 
TGCTACTCTA AAGTGATGGC AAAATGGATT CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Scel ( NM_022886.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_075024.2 Mus musculus sciellin (Scel), mRNA.

Price & Availability↑

CloneID	OMu15713
Clone ID Related Accession (Same CDS sequence)	XM_006519355.2 , XM_006519355.4 , XM_006519355.3 , NM_022886.2
Accession Version	NM_022886.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1959bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Mus musculus(house mouse)
Product	sciellin
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY775767.1, AF245700.2 and AC125201.4. On Jul 16, 2005 this sequence version replaced NM_022886.1. ##Evidence-Data-START## Transcript exon combination :: AF245700.2, BC111874.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849385 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCAAATT TTTCCTCTAG AAAAAAGTCT CCAACTGGAA ATGATCTGAA GAGCGCCCGG 
GGAGCTGAGC TGAGACAACA GGGTTTTCAG GACGTGAACA AAAGAAGGGC TTTCTTACAG 
GACAACAGCT GGATAAAGAA GCCCCCAGAG GAAGAACAAG ACGGAAATTA TGGCAGGGTG 
GTGCTCAACC GGCACAACTC CCATGATGCC TTGGACAGGA AATTAATAGA GAGAGATGAG 
CCAAAAGCCA CAATTAGCCG GTACAGATCT GAGGACATGC TGGACAGAAC ACTGTCCTCA 
TTTAGAACGC CACAATCAAC CAAGACGCCA GCTGTCAGCT CCTTCAATGC AAACACTACC 
GCCACAGCTT CAACCCCCGC TACCACTCCT GTGAAGAAAA AGAGACAGTC CTGGTTCCCA 
CCACCCCCTC CTGGTCACAA TGCCTCTCCA AGCACAGCAG CCAGCAGAAG GGACCCAGCT 
CTGCACCCTC CACTCCCCCC AAAGCCCTGT TCTCCCATTG CTTCTCCTAA ACCATTGGGG 
CGGACTAATA GGCAGATACA TGCAGCTACA GCGGGGGCAT GTGGAGAAAC AGAACGACAT 
GCTGAAAGAA ATATAAGGAC TGAGGATCTC GATGACATCA TCAGGGTGGC GGCTGCACTT 
CAGAAAACTG ACAAGGGTGA AGAATTGGAT AATCTCATCA GAATGAACAA AAGCTTGAAC 
AGAAACCAAG GTCTCGATGG CCTCTTCCGA GCAAACCTGA AGGCACAGCA ATTGGACAAA 
AGAGCCCAGA GTCTTGAAAG CCTGATCTAT ATGAACACAC AGACGGACAG AGATGGCAAA 
GGAAATCAAG CCTTCGGGAG CCTTAAGAAA ATCAACCAAA GGGCTGACCC CGACAGAAGA 
AGCCAAGATC TCAGATCAGT TATCAGAACA CATGCCACGG CTGAGAGAAT CGGCAGAAGA 
AAACAAGACC TCGATGGACT GATTAAAGTG AATCCTGACA CAAATAAAAA TATGAAGAGG 
GGCAAGAGCC TTGACAATCT TATCAAAGTC ACCCCGGAAG TAAACAGAAG CAACAAAGGG 
GGTCCAAGTC TTGACAATTT CACCAAAGGA GTTCCTGCAA GGAGCAGAGC TAACCAAAGG 
GACCAAGACC TGGACAGTCT TATCAAGGTG ACCCCCTCAG CAAACAGAAG CAGCCAGCAC 
AGTCTTGATG AACTTATTAA TACAAGCCCC CAAACTATCA AAACTACTGC TAGACACCAA 
GATCTCGATA AGTTCATCAA AGTAAATCCT GATGTTCTCA CAAACAACCA AAGAAACCAT 
GATGTAGACA GCACCATCAG AGGGAATCCT ACAGGGACCA GATGCGAGCA AAGCGAAGAG 
TTGGACAATC TTATCAAAGT GAAGCCTTCT GCTCTCAGAA ACACAAATGG AGGCCAGGAC 
CTAGAAAGCT TAACTGAAGT AAATTCTCAT GTGGCTGAGA AGAATGGGAG GATAGATGGC 
CAAGCCAATG GATTGACTAA CAGTCTGTTC AAGGAGTCCA CAAGAGCCTC GGTCTATTCT 
TATGAAGCCA GAAACAGTCT CAGCTCCAAT TCTGGAAACA AGAATGGTGG ACCCAAGGAC 
ACTGTTGTTT ATACAAGGAC ATATGTGGAG AACAGTAAAT CACCAAAGGA TGGTTATCAG 
GAAAATATCT CAGGAAAATA TATACAAACC GTTTATTCAA CTTCAGATAG GTCTGTCATT 
GAAAGAGACA TGTGCACTTA CTGCCGCAAG CCCTTGGGGG TGGAAACCAA GATGATTTTA 
GATGAATTAC AGATCTGTTG CCATTCCACC TGCTTTAAGT GTGAAATATG CAAACGGCCT 
CTGGAAAATT TACAGGCAGG AGACAGTATT TGGATCTATA GGCAGACGAT ACACTGTGAG 
CCCTGCTACT CTAAAGTGAT GGCAAAATGG ATTCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_075024.2
CDS	148..2106
Translation	MSNFSSRKKSPTGNDLKSARGAELRQQGFQDVNKRRAFLQDNSWIKKPPEEEQDGNYGRVVLNRHNSHDALDRKLIERDEPKATISRYRSEDMLDRTLSSFRTPQSTKTPAVSSFNANTTATASTPATTPVKKKRQSWFPPPPPGHNASPSTAASRRDPALHPPLPPKPCSPIASPKPLGRTNRQIHAATAGACGETERHAERNIRTEDLDDIIRVAAALQKTDKGEELDNLIRMNKSLNRNQGLDGLFRANLKAQQLDKRAQSLESLIYMNTQTDRDGKGNQAFGSLKKINQRADPDRRSQDLRSVIRTHATAERIGRRKQDLDGLIKVNPDTNKNMKRGKSLDNLIKVTPEVNRSNKGGPSLDNFTKGVPARSRANQRDQDLDSLIKVTPSANRSSQHSLDELINTSPQTIKTTARHQDLDKFIKVNPDVLTNNQRNHDVDSTIRGNPTGTRCEQSEELDNLIKVKPSALRNTNGGQDLESLTEVNSHVAEKNGRIDGQANGLTNSLFKESTRASVYSYEARNSLSSNSGNKNGGPKDTVVYTRTYVENSKSPKDGYQENISGKYIQTVYSTSDRSVIERDMCTYCRKPLGVETKMILDELQICCHSTCFKCEICKRPLENLQAGDSIWIYRQTIHCEPCYSKVMAKWIQ

Target ORF information:

RefSeq Version	NM_022886.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus sciellin (Scel), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022886.2

ORF Insert Sequence:

ATGTCAAATT TTTCCTCTAG AAAAAAGTCT CCAACTGGAA ATGATCTGAA GAGCGCCCGG 
GGAGCTGAGC TGAGACAACA GGGTTTTCAG GACGTGAACA AAAGAAGGGC TTTCTTACAG 
GACAACAGCT GGATAAAGAA GCCCCCAGAG GAAGAACAAG ACGGAAATTA TGGCAGGGTG 
GTGCTCAACC GGCACAACTC CCATGATGCC TTGGACAGGA AATTAATAGA GAGAGATGAG 
CCAAAAGCCA CAATTAGCCG GTACAGATCT GAGGACATGC TGGACAGAAC ACTGTCCTCA 
TTTAGAACGC CACAATCAAC CAAGACGCCA GCTGTCAGCT CCTTCAATGC AAACACTACC 
GCCACAGCTT CAACCCCCGC TACCACTCCT GTGAAGAAAA AGAGACAGTC CTGGTTCCCA 
CCACCCCCTC CTGGTCACAA TGCCTCTCCA AGCACAGCAG CCAGCAGAAG GGACCCAGCT 
CTGCACCCTC CACTCCCCCC AAAGCCCTGT TCTCCCATTG CTTCTCCTAA ACCATTGGGG 
CGGACTAATA GGCAGATACA TGCAGCTACA GCGGGGGCAT GTGGAGAAAC AGAACGACAT 
GCTGAAAGAA ATATAAGGAC TGAGGATCTC GATGACATCA TCAGGGTGGC GGCTGCACTT 
CAGAAAACTG ACAAGGGTGA AGAATTGGAT AATCTCATCA GAATGAACAA AAGCTTGAAC 
AGAAACCAAG GTCTCGATGG CCTCTTCCGA GCAAACCTGA AGGCACAGCA ATTGGACAAA 
AGAGCCCAGA GTCTTGAAAG CCTGATCTAT ATGAACACAC AGACGGACAG AGATGGCAAA 
GGAAATCAAG CCTTCGGGAG CCTTAAGAAA ATCAACCAAA GGGCTGACCC CGACAGAAGA 
AGCCAAGATC TCAGATCAGT TATCAGAACA CATGCCACGG CTGAGAGAAT CGGCAGAAGA 
AAACAAGACC TCGATGGACT GATTAAAGTG AATCCTGACA CAAATAAAAA TATGAAGAGG 
GGCAAGAGCC TTGACAATCT TATCAAAGTC ACCCCGGAAG TAAACAGAAG CAACAAAGGG 
GGTCCAAGTC TTGACAATTT CACCAAAGGA GTTCCTGCAA GGAGCAGAGC TAACCAAAGG 
GACCAAGACC TGGACAGTCT TATCAAGGTG ACCCCCTCAG CAAACAGAAG CAGCCAGCAC 
AGTCTTGATG AACTTATTAA TACAAGCCCC CAAACTATCA AAACTACTGC TAGACACCAA 
GATCTCGATA AGTTCATCAA AGTAAATCCT GATGTTCTCA CAAACAACCA AAGAAACCAT 
GATGTAGACA GCACCATCAG AGGGAATCCT ACAGGGACCA GATGCGAGCA AAGCGAAGAG 
TTGGACAATC TTATCAAAGT GAAGCCTTCT GCTCTCAGAA ACACAAATGG AGGCCAGGAC 
CTAGAAAGCT TAACTGAAGT AAATTCTCAT GTGGCTGAGA AGAATGGGAG GATAGATGGC 
CAAGCCAATG GATTGACTAA CAGTCTGTTC AAGGAGTCCA CAAGAGCCTC GGTCTATTCT 
TATGAAGCCA GAAACAGTCT CAGCTCCAAT TCTGGAAACA AGAATGGTGG ACCCAAGGAC 
ACTGTTGTTT ATACAAGGAC ATATGTGGAG AACAGTAAAT CACCAAAGGA TGGTTATCAG 
GAAAATATCT CAGGAAAATA TATACAAACC GTTTATTCAA CTTCAGATAG GTCTGTCATT 
GAAAGAGACA TGTGCACTTA CTGCCGCAAG CCCTTGGGGG TGGAAACCAA GATGATTTTA 
GATGAATTAC AGATCTGTTG CCATTCCACC TGCTTTAAGT GTGAAATATG CAAACGGCCT 
CTGGAAAATT TACAGGCAGG AGACAGTATT TGGATCTATA GGCAGACGAT ACACTGTGAG 
CCCTGCTACT CTAAAGTGAT GGCAAAATGG ATTCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Scel ( XM_006519355.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006519418.1 Mus musculus sciellin (Scel), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu15713
Clone ID Related Accession (Same CDS sequence)	XM_006519355.2 , XM_006519355.4 , XM_006519355.3 , NM_022886.2
Accession Version	XM_006519355.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1959bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	sciellin isoform X1
Comment

ATGTCAAATT TTTCCTCTAG AAAAAAGTCT CCAACTGGAA ATGATCTGAA GAGCGCCCGG 
GGAGCTGAGC TGAGACAACA GGGTTTTCAG GACGTGAACA AAAGAAGGGC TTTCTTACAG 
GACAACAGCT GGATAAAGAA GCCCCCAGAG GAAGAACAAG ACGGAAATTA TGGCAGGGTG 
GTGCTCAACC GGCACAACTC CCATGATGCC TTGGACAGGA AATTAATAGA GAGAGATGAG 
CCAAAAGCCA CAATTAGCCG GTACAGATCT GAGGACATGC TGGACAGAAC ACTGTCCTCA 
TTTAGAACGC CACAATCAAC CAAGACGCCA GCTGTCAGCT CCTTCAATGC AAACACTACC 
GCCACAGCTT CAACCCCCGC TACCACTCCT GTGAAGAAAA AGAGACAGTC CTGGTTCCCA 
CCACCCCCTC CTGGTCACAA TGCCTCTCCA AGCACAGCAG CCAGCAGAAG GGACCCAGCT 
CTGCACCCTC CACTCCCCCC AAAGCCCTGT TCTCCCATTG CTTCTCCTAA ACCATTGGGG 
CGGACTAATA GGCAGATACA TGCAGCTACA GCGGGGGCAT GTGGAGAAAC AGAACGACAT 
GCTGAAAGAA ATATAAGGAC TGAGGATCTC GATGACATCA TCAGGGTGGC GGCTGCACTT 
CAGAAAACTG ACAAGGGTGA AGAATTGGAT AATCTCATCA GAATGAACAA AAGCTTGAAC 
AGAAACCAAG GTCTCGATGG CCTCTTCCGA GCAAACCTGA AGGCACAGCA ATTGGACAAA 
AGAGCCCAGA GTCTTGAAAG CCTGATCTAT ATGAACACAC AGACGGACAG AGATGGCAAA 
GGAAATCAAG CCTTCGGGAG CCTTAAGAAA ATCAACCAAA GGGCTGACCC CGACAGAAGA 
AGCCAAGATC TCAGATCAGT TATCAGAACA CATGCCACGG CTGAGAGAAT CGGCAGAAGA 
AAACAAGACC TCGATGGACT GATTAAAGTG AATCCTGACA CAAATAAAAA TATGAAGAGG 
GGCAAGAGCC TTGACAATCT TATCAAAGTC ACCCCGGAAG TAAACAGAAG CAACAAAGGG 
GGTCCAAGTC TTGACAATTT CACCAAAGGA GTTCCTGCAA GGAGCAGAGC TAACCAAAGG 
GACCAAGACC TGGACAGTCT TATCAAGGTG ACCCCCTCAG CAAACAGAAG CAGCCAGCAC 
AGTCTTGATG AACTTATTAA TACAAGCCCC CAAACTATCA AAACTACTGC TAGACACCAA 
GATCTCGATA AGTTCATCAA AGTAAATCCT GATGTTCTCA CAAACAACCA AAGAAACCAT 
GATGTAGACA GCACCATCAG AGGGAATCCT ACAGGGACCA GATGCGAGCA AAGCGAAGAG 
TTGGACAATC TTATCAAAGT GAAGCCTTCT GCTCTCAGAA ACACAAATGG AGGCCAGGAC 
CTAGAAAGCT TAACTGAAGT AAATTCTCAT GTGGCTGAGA AGAATGGGAG GATAGATGGC 
CAAGCCAATG GATTGACTAA CAGTCTGTTC AAGGAGTCCA CAAGAGCCTC GGTCTATTCT 
TATGAAGCCA GAAACAGTCT CAGCTCCAAT TCTGGAAACA AGAATGGTGG ACCCAAGGAC 
ACTGTTGTTT ATACAAGGAC ATATGTGGAG AACAGTAAAT CACCAAAGGA TGGTTATCAG 
GAAAATATCT CAGGAAAATA TATACAAACC GTTTATTCAA CTTCAGATAG GTCTGTCATT 
GAAAGAGACA TGTGCACTTA CTGCCGCAAG CCCTTGGGGG TGGAAACCAA GATGATTTTA 
GATGAATTAC AGATCTGTTG CCATTCCACC TGCTTTAAGT GTGAAATATG CAAACGGCCT 
CTGGAAAATT TACAGGCAGG AGACAGTATT TGGATCTATA GGCAGACGAT ACACTGTGAG 
CCCTGCTACT CTAAAGTGAT GGCAAAATGG ATTCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_006519418.1

Translation

MSNFSSRKKSPTGNDLKSARGAELRQQGFQDVNKRRAFLQDNSWIKKPPEEEQDGNYGRVVLNRHNSHDALDRKLIERDEPKATISRYRSEDMLDRTLSSFRTPQSTKTPAVSSFNANTTATASTPATTPVKKKRQSWFPPPPPGHNASPSTAASRRDPALHPPLPPKPCSPIASPKPLGRTNRQIHAATAGACGETERHAERNIRTEDLDDIIRVAAALQKTDKGEELDNLIRMNKSLNRNQGLDGLFRANLKAQQLDKRAQSLESLIYMNTQTDRDGKGNQAFGSLKKINQRADPDRRSQDLRSVIRTHATAERIGRRKQDLDGLIKVNPDTNKNMKRGKSLDNLIKVTPEVNRSNKGGPSLDNFTKGVPARSRANQRDQDLDSLIKVTPSANRSSQHSLDELINTSPQTIKTTARHQDLDKFIKVNPDVLTNNQRNHDVDSTIRGNPTGTRCEQSEELDNLIKVKPSALRNTNGGQDLESLTEVNSHVAEKNGRIDGQANGLTNSLFKESTRASVYSYEARNSLSSNSGNKNGGPKDTVVYTRTYVENSKSPKDGYQENISGKYIQTVYSTSDRSVIERDMCTYCRKPLGVETKMILDELQICCHSTCFKCEICKRPLENLQAGDSIWIYRQTIHCEPCYSKVMAKWIQ

Target ORF information:

RefSeq Version	XM_006519355.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus sciellin (Scel), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006519355.2

ORF Insert Sequence:

ATGTCAAATT TTTCCTCTAG AAAAAAGTCT CCAACTGGAA ATGATCTGAA GAGCGCCCGG 
GGAGCTGAGC TGAGACAACA GGGTTTTCAG GACGTGAACA AAAGAAGGGC TTTCTTACAG 
GACAACAGCT GGATAAAGAA GCCCCCAGAG GAAGAACAAG ACGGAAATTA TGGCAGGGTG 
GTGCTCAACC GGCACAACTC CCATGATGCC TTGGACAGGA AATTAATAGA GAGAGATGAG 
CCAAAAGCCA CAATTAGCCG GTACAGATCT GAGGACATGC TGGACAGAAC ACTGTCCTCA 
TTTAGAACGC CACAATCAAC CAAGACGCCA GCTGTCAGCT CCTTCAATGC AAACACTACC 
GCCACAGCTT CAACCCCCGC TACCACTCCT GTGAAGAAAA AGAGACAGTC CTGGTTCCCA 
CCACCCCCTC CTGGTCACAA TGCCTCTCCA AGCACAGCAG CCAGCAGAAG GGACCCAGCT 
CTGCACCCTC CACTCCCCCC AAAGCCCTGT TCTCCCATTG CTTCTCCTAA ACCATTGGGG 
CGGACTAATA GGCAGATACA TGCAGCTACA GCGGGGGCAT GTGGAGAAAC AGAACGACAT 
GCTGAAAGAA ATATAAGGAC TGAGGATCTC GATGACATCA TCAGGGTGGC GGCTGCACTT 
CAGAAAACTG ACAAGGGTGA AGAATTGGAT AATCTCATCA GAATGAACAA AAGCTTGAAC 
AGAAACCAAG GTCTCGATGG CCTCTTCCGA GCAAACCTGA AGGCACAGCA ATTGGACAAA 
AGAGCCCAGA GTCTTGAAAG CCTGATCTAT ATGAACACAC AGACGGACAG AGATGGCAAA 
GGAAATCAAG CCTTCGGGAG CCTTAAGAAA ATCAACCAAA GGGCTGACCC CGACAGAAGA 
AGCCAAGATC TCAGATCAGT TATCAGAACA CATGCCACGG CTGAGAGAAT CGGCAGAAGA 
AAACAAGACC TCGATGGACT GATTAAAGTG AATCCTGACA CAAATAAAAA TATGAAGAGG 
GGCAAGAGCC TTGACAATCT TATCAAAGTC ACCCCGGAAG TAAACAGAAG CAACAAAGGG 
GGTCCAAGTC TTGACAATTT CACCAAAGGA GTTCCTGCAA GGAGCAGAGC TAACCAAAGG 
GACCAAGACC TGGACAGTCT TATCAAGGTG ACCCCCTCAG CAAACAGAAG CAGCCAGCAC 
AGTCTTGATG AACTTATTAA TACAAGCCCC CAAACTATCA AAACTACTGC TAGACACCAA 
GATCTCGATA AGTTCATCAA AGTAAATCCT GATGTTCTCA CAAACAACCA AAGAAACCAT 
GATGTAGACA GCACCATCAG AGGGAATCCT ACAGGGACCA GATGCGAGCA AAGCGAAGAG 
TTGGACAATC TTATCAAAGT GAAGCCTTCT GCTCTCAGAA ACACAAATGG AGGCCAGGAC 
CTAGAAAGCT TAACTGAAGT AAATTCTCAT GTGGCTGAGA AGAATGGGAG GATAGATGGC 
CAAGCCAATG GATTGACTAA CAGTCTGTTC AAGGAGTCCA CAAGAGCCTC GGTCTATTCT 
TATGAAGCCA GAAACAGTCT CAGCTCCAAT TCTGGAAACA AGAATGGTGG ACCCAAGGAC 
ACTGTTGTTT ATACAAGGAC ATATGTGGAG AACAGTAAAT CACCAAAGGA TGGTTATCAG 
GAAAATATCT CAGGAAAATA TATACAAACC GTTTATTCAA CTTCAGATAG GTCTGTCATT 
GAAAGAGACA TGTGCACTTA CTGCCGCAAG CCCTTGGGGG TGGAAACCAA GATGATTTTA 
GATGAATTAC AGATCTGTTG CCATTCCACC TGCTTTAAGT GTGAAATATG CAAACGGCCT 
CTGGAAAATT TACAGGCAGG AGACAGTATT TGGATCTATA GGCAGACGAT ACACTGTGAG 
CCCTGCTACT CTAAAGTGAT GGCAAAATGG ATTCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Scel ( XM_006519355.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006519418.1 Mus musculus sciellin (Scel), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu15713
Clone ID Related Accession (Same CDS sequence)	XM_006519355.2 , XM_006519355.4 , XM_006519355.3 , NM_022886.2
Accession Version	XM_006519355.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1959bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	sciellin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006519355.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAATT TTTCCTCTAG AAAAAAGTCT CCAACTGGAA ATGATCTGAA GAGCGCCCGG 
GGAGCTGAGC TGAGACAACA GGGTTTTCAG GACGTGAACA AAAGAAGGGC TTTCTTACAG 
GACAACAGCT GGATAAAGAA GCCCCCAGAG GAAGAACAAG ACGGAAATTA TGGCAGGGTG 
GTGCTCAACC GGCACAACTC CCATGATGCC TTGGACAGGA AATTAATAGA GAGAGATGAG 
CCAAAAGCCA CAATTAGCCG GTACAGATCT GAGGACATGC TGGACAGAAC ACTGTCCTCA 
TTTAGAACGC CACAATCAAC CAAGACGCCA GCTGTCAGCT CCTTCAATGC AAACACTACC 
GCCACAGCTT CAACCCCCGC TACCACTCCT GTGAAGAAAA AGAGACAGTC CTGGTTCCCA 
CCACCCCCTC CTGGTCACAA TGCCTCTCCA AGCACAGCAG CCAGCAGAAG GGACCCAGCT 
CTGCACCCTC CACTCCCCCC AAAGCCCTGT TCTCCCATTG CTTCTCCTAA ACCATTGGGG 
CGGACTAATA GGCAGATACA TGCAGCTACA GCGGGGGCAT GTGGAGAAAC AGAACGACAT 
GCTGAAAGAA ATATAAGGAC TGAGGATCTC GATGACATCA TCAGGGTGGC GGCTGCACTT 
CAGAAAACTG ACAAGGGTGA AGAATTGGAT AATCTCATCA GAATGAACAA AAGCTTGAAC 
AGAAACCAAG GTCTCGATGG CCTCTTCCGA GCAAACCTGA AGGCACAGCA ATTGGACAAA 
AGAGCCCAGA GTCTTGAAAG CCTGATCTAT ATGAACACAC AGACGGACAG AGATGGCAAA 
GGAAATCAAG CCTTCGGGAG CCTTAAGAAA ATCAACCAAA GGGCTGACCC CGACAGAAGA 
AGCCAAGATC TCAGATCAGT TATCAGAACA CATGCCACGG CTGAGAGAAT CGGCAGAAGA 
AAACAAGACC TCGATGGACT GATTAAAGTG AATCCTGACA CAAATAAAAA TATGAAGAGG 
GGCAAGAGCC TTGACAATCT TATCAAAGTC ACCCCGGAAG TAAACAGAAG CAACAAAGGG 
GGTCCAAGTC TTGACAATTT CACCAAAGGA GTTCCTGCAA GGAGCAGAGC TAACCAAAGG 
GACCAAGACC TGGACAGTCT TATCAAGGTG ACCCCCTCAG CAAACAGAAG CAGCCAGCAC 
AGTCTTGATG AACTTATTAA TACAAGCCCC CAAACTATCA AAACTACTGC TAGACACCAA 
GATCTCGATA AGTTCATCAA AGTAAATCCT GATGTTCTCA CAAACAACCA AAGAAACCAT 
GATGTAGACA GCACCATCAG AGGGAATCCT ACAGGGACCA GATGCGAGCA AAGCGAAGAG 
TTGGACAATC TTATCAAAGT GAAGCCTTCT GCTCTCAGAA ACACAAATGG AGGCCAGGAC 
CTAGAAAGCT TAACTGAAGT AAATTCTCAT GTGGCTGAGA AGAATGGGAG GATAGATGGC 
CAAGCCAATG GATTGACTAA CAGTCTGTTC AAGGAGTCCA CAAGAGCCTC GGTCTATTCT 
TATGAAGCCA GAAACAGTCT CAGCTCCAAT TCTGGAAACA AGAATGGTGG ACCCAAGGAC 
ACTGTTGTTT ATACAAGGAC ATATGTGGAG AACAGTAAAT CACCAAAGGA TGGTTATCAG 
GAAAATATCT CAGGAAAATA TATACAAACC GTTTATTCAA CTTCAGATAG GTCTGTCATT 
GAAAGAGACA TGTGCACTTA CTGCCGCAAG CCCTTGGGGG TGGAAACCAA GATGATTTTA 
GATGAATTAC AGATCTGTTG CCATTCCACC TGCTTTAAGT GTGAAATATG CAAACGGCCT 
CTGGAAAATT TACAGGCAGG AGACAGTATT TGGATCTATA GGCAGACGAT ACACTGTGAG 
CCCTGCTACT CTAAAGTGAT GGCAAAATGG ATTCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006519418.1
CDS	184..2142
Translation	MSNFSSRKKSPTGNDLKSARGAELRQQGFQDVNKRRAFLQDNSWIKKPPEEEQDGNYGRVVLNRHNSHDALDRKLIERDEPKATISRYRSEDMLDRTLSSFRTPQSTKTPAVSSFNANTTATASTPATTPVKKKRQSWFPPPPPGHNASPSTAASRRDPALHPPLPPKPCSPIASPKPLGRTNRQIHAATAGACGETERHAERNIRTEDLDDIIRVAAALQKTDKGEELDNLIRMNKSLNRNQGLDGLFRANLKAQQLDKRAQSLESLIYMNTQTDRDGKGNQAFGSLKKINQRADPDRRSQDLRSVIRTHATAERIGRRKQDLDGLIKVNPDTNKNMKRGKSLDNLIKVTPEVNRSNKGGPSLDNFTKGVPARSRANQRDQDLDSLIKVTPSANRSSQHSLDELINTSPQTIKTTARHQDLDKFIKVNPDVLTNNQRNHDVDSTIRGNPTGTRCEQSEELDNLIKVKPSALRNTNGGQDLESLTEVNSHVAEKNGRIDGQANGLTNSLFKESTRASVYSYEARNSLSSNSGNKNGGPKDTVVYTRTYVENSKSPKDGYQENISGKYIQTVYSTSDRSVIERDMCTYCRKPLGVETKMILDELQICCHSTCFKCEICKRPLENLQAGDSIWIYRQTIHCEPCYSKVMAKWIQ

Target ORF information:

RefSeq Version	XM_006519355.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus sciellin (Scel), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006519355.4

ORF Insert Sequence:

ATGTCAAATT TTTCCTCTAG AAAAAAGTCT CCAACTGGAA ATGATCTGAA GAGCGCCCGG 
GGAGCTGAGC TGAGACAACA GGGTTTTCAG GACGTGAACA AAAGAAGGGC TTTCTTACAG 
GACAACAGCT GGATAAAGAA GCCCCCAGAG GAAGAACAAG ACGGAAATTA TGGCAGGGTG 
GTGCTCAACC GGCACAACTC CCATGATGCC TTGGACAGGA AATTAATAGA GAGAGATGAG 
CCAAAAGCCA CAATTAGCCG GTACAGATCT GAGGACATGC TGGACAGAAC ACTGTCCTCA 
TTTAGAACGC CACAATCAAC CAAGACGCCA GCTGTCAGCT CCTTCAATGC AAACACTACC 
GCCACAGCTT CAACCCCCGC TACCACTCCT GTGAAGAAAA AGAGACAGTC CTGGTTCCCA 
CCACCCCCTC CTGGTCACAA TGCCTCTCCA AGCACAGCAG CCAGCAGAAG GGACCCAGCT 
CTGCACCCTC CACTCCCCCC AAAGCCCTGT TCTCCCATTG CTTCTCCTAA ACCATTGGGG 
CGGACTAATA GGCAGATACA TGCAGCTACA GCGGGGGCAT GTGGAGAAAC AGAACGACAT 
GCTGAAAGAA ATATAAGGAC TGAGGATCTC GATGACATCA TCAGGGTGGC GGCTGCACTT 
CAGAAAACTG ACAAGGGTGA AGAATTGGAT AATCTCATCA GAATGAACAA AAGCTTGAAC 
AGAAACCAAG GTCTCGATGG CCTCTTCCGA GCAAACCTGA AGGCACAGCA ATTGGACAAA 
AGAGCCCAGA GTCTTGAAAG CCTGATCTAT ATGAACACAC AGACGGACAG AGATGGCAAA 
GGAAATCAAG CCTTCGGGAG CCTTAAGAAA ATCAACCAAA GGGCTGACCC CGACAGAAGA 
AGCCAAGATC TCAGATCAGT TATCAGAACA CATGCCACGG CTGAGAGAAT CGGCAGAAGA 
AAACAAGACC TCGATGGACT GATTAAAGTG AATCCTGACA CAAATAAAAA TATGAAGAGG 
GGCAAGAGCC TTGACAATCT TATCAAAGTC ACCCCGGAAG TAAACAGAAG CAACAAAGGG 
GGTCCAAGTC TTGACAATTT CACCAAAGGA GTTCCTGCAA GGAGCAGAGC TAACCAAAGG 
GACCAAGACC TGGACAGTCT TATCAAGGTG ACCCCCTCAG CAAACAGAAG CAGCCAGCAC 
AGTCTTGATG AACTTATTAA TACAAGCCCC CAAACTATCA AAACTACTGC TAGACACCAA 
GATCTCGATA AGTTCATCAA AGTAAATCCT GATGTTCTCA CAAACAACCA AAGAAACCAT 
GATGTAGACA GCACCATCAG AGGGAATCCT ACAGGGACCA GATGCGAGCA AAGCGAAGAG 
TTGGACAATC TTATCAAAGT GAAGCCTTCT GCTCTCAGAA ACACAAATGG AGGCCAGGAC 
CTAGAAAGCT TAACTGAAGT AAATTCTCAT GTGGCTGAGA AGAATGGGAG GATAGATGGC 
CAAGCCAATG GATTGACTAA CAGTCTGTTC AAGGAGTCCA CAAGAGCCTC GGTCTATTCT 
TATGAAGCCA GAAACAGTCT CAGCTCCAAT TCTGGAAACA AGAATGGTGG ACCCAAGGAC 
ACTGTTGTTT ATACAAGGAC ATATGTGGAG AACAGTAAAT CACCAAAGGA TGGTTATCAG 
GAAAATATCT CAGGAAAATA TATACAAACC GTTTATTCAA CTTCAGATAG GTCTGTCATT 
GAAAGAGACA TGTGCACTTA CTGCCGCAAG CCCTTGGGGG TGGAAACCAA GATGATTTTA 
GATGAATTAC AGATCTGTTG CCATTCCACC TGCTTTAAGT GTGAAATATG CAAACGGCCT 
CTGGAAAATT TACAGGCAGG AGACAGTATT TGGATCTATA GGCAGACGAT ACACTGTGAG 
CCCTGCTACT CTAAAGTGAT GGCAAAATGG ATTCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Scel ( XM_006519355.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006519418.1 Mus musculus sciellin (Scel), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu15713
Clone ID Related Accession (Same CDS sequence)	XM_006519355.2 , XM_006519355.4 , XM_006519355.3 , NM_022886.2
Accession Version	XM_006519355.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1959bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	sciellin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006519355.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAATT TTTCCTCTAG AAAAAAGTCT CCAACTGGAA ATGATCTGAA GAGCGCCCGG 
GGAGCTGAGC TGAGACAACA GGGTTTTCAG GACGTGAACA AAAGAAGGGC TTTCTTACAG 
GACAACAGCT GGATAAAGAA GCCCCCAGAG GAAGAACAAG ACGGAAATTA TGGCAGGGTG 
GTGCTCAACC GGCACAACTC CCATGATGCC TTGGACAGGA AATTAATAGA GAGAGATGAG 
CCAAAAGCCA CAATTAGCCG GTACAGATCT GAGGACATGC TGGACAGAAC ACTGTCCTCA 
TTTAGAACGC CACAATCAAC CAAGACGCCA GCTGTCAGCT CCTTCAATGC AAACACTACC 
GCCACAGCTT CAACCCCCGC TACCACTCCT GTGAAGAAAA AGAGACAGTC CTGGTTCCCA 
CCACCCCCTC CTGGTCACAA TGCCTCTCCA AGCACAGCAG CCAGCAGAAG GGACCCAGCT 
CTGCACCCTC CACTCCCCCC AAAGCCCTGT TCTCCCATTG CTTCTCCTAA ACCATTGGGG 
CGGACTAATA GGCAGATACA TGCAGCTACA GCGGGGGCAT GTGGAGAAAC AGAACGACAT 
GCTGAAAGAA ATATAAGGAC TGAGGATCTC GATGACATCA TCAGGGTGGC GGCTGCACTT 
CAGAAAACTG ACAAGGGTGA AGAATTGGAT AATCTCATCA GAATGAACAA AAGCTTGAAC 
AGAAACCAAG GTCTCGATGG CCTCTTCCGA GCAAACCTGA AGGCACAGCA ATTGGACAAA 
AGAGCCCAGA GTCTTGAAAG CCTGATCTAT ATGAACACAC AGACGGACAG AGATGGCAAA 
GGAAATCAAG CCTTCGGGAG CCTTAAGAAA ATCAACCAAA GGGCTGACCC CGACAGAAGA 
AGCCAAGATC TCAGATCAGT TATCAGAACA CATGCCACGG CTGAGAGAAT CGGCAGAAGA 
AAACAAGACC TCGATGGACT GATTAAAGTG AATCCTGACA CAAATAAAAA TATGAAGAGG 
GGCAAGAGCC TTGACAATCT TATCAAAGTC ACCCCGGAAG TAAACAGAAG CAACAAAGGG 
GGTCCAAGTC TTGACAATTT CACCAAAGGA GTTCCTGCAA GGAGCAGAGC TAACCAAAGG 
GACCAAGACC TGGACAGTCT TATCAAGGTG ACCCCCTCAG CAAACAGAAG CAGCCAGCAC 
AGTCTTGATG AACTTATTAA TACAAGCCCC CAAACTATCA AAACTACTGC TAGACACCAA 
GATCTCGATA AGTTCATCAA AGTAAATCCT GATGTTCTCA CAAACAACCA AAGAAACCAT 
GATGTAGACA GCACCATCAG AGGGAATCCT ACAGGGACCA GATGCGAGCA AAGCGAAGAG 
TTGGACAATC TTATCAAAGT GAAGCCTTCT GCTCTCAGAA ACACAAATGG AGGCCAGGAC 
CTAGAAAGCT TAACTGAAGT AAATTCTCAT GTGGCTGAGA AGAATGGGAG GATAGATGGC 
CAAGCCAATG GATTGACTAA CAGTCTGTTC AAGGAGTCCA CAAGAGCCTC GGTCTATTCT 
TATGAAGCCA GAAACAGTCT CAGCTCCAAT TCTGGAAACA AGAATGGTGG ACCCAAGGAC 
ACTGTTGTTT ATACAAGGAC ATATGTGGAG AACAGTAAAT CACCAAAGGA TGGTTATCAG 
GAAAATATCT CAGGAAAATA TATACAAACC GTTTATTCAA CTTCAGATAG GTCTGTCATT 
GAAAGAGACA TGTGCACTTA CTGCCGCAAG CCCTTGGGGG TGGAAACCAA GATGATTTTA 
GATGAATTAC AGATCTGTTG CCATTCCACC TGCTTTAAGT GTGAAATATG CAAACGGCCT 
CTGGAAAATT TACAGGCAGG AGACAGTATT TGGATCTATA GGCAGACGAT ACACTGTGAG 
CCCTGCTACT CTAAAGTGAT GGCAAAATGG ATTCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006519418.1
CDS	185..2143
Translation	MSNFSSRKKSPTGNDLKSARGAELRQQGFQDVNKRRAFLQDNSWIKKPPEEEQDGNYGRVVLNRHNSHDALDRKLIERDEPKATISRYRSEDMLDRTLSSFRTPQSTKTPAVSSFNANTTATASTPATTPVKKKRQSWFPPPPPGHNASPSTAASRRDPALHPPLPPKPCSPIASPKPLGRTNRQIHAATAGACGETERHAERNIRTEDLDDIIRVAAALQKTDKGEELDNLIRMNKSLNRNQGLDGLFRANLKAQQLDKRAQSLESLIYMNTQTDRDGKGNQAFGSLKKINQRADPDRRSQDLRSVIRTHATAERIGRRKQDLDGLIKVNPDTNKNMKRGKSLDNLIKVTPEVNRSNKGGPSLDNFTKGVPARSRANQRDQDLDSLIKVTPSANRSSQHSLDELINTSPQTIKTTARHQDLDKFIKVNPDVLTNNQRNHDVDSTIRGNPTGTRCEQSEELDNLIKVKPSALRNTNGGQDLESLTEVNSHVAEKNGRIDGQANGLTNSLFKESTRASVYSYEARNSLSSNSGNKNGGPKDTVVYTRTYVENSKSPKDGYQENISGKYIQTVYSTSDRSVIERDMCTYCRKPLGVETKMILDELQICCHSTCFKCEICKRPLENLQAGDSIWIYRQTIHCEPCYSKVMAKWIQ

Target ORF information:

RefSeq Version	XM_006519355.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus sciellin (Scel), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006519355.3

ORF Insert Sequence:

ATGTCAAATT TTTCCTCTAG AAAAAAGTCT CCAACTGGAA ATGATCTGAA GAGCGCCCGG 
GGAGCTGAGC TGAGACAACA GGGTTTTCAG GACGTGAACA AAAGAAGGGC TTTCTTACAG 
GACAACAGCT GGATAAAGAA GCCCCCAGAG GAAGAACAAG ACGGAAATTA TGGCAGGGTG 
GTGCTCAACC GGCACAACTC CCATGATGCC TTGGACAGGA AATTAATAGA GAGAGATGAG 
CCAAAAGCCA CAATTAGCCG GTACAGATCT GAGGACATGC TGGACAGAAC ACTGTCCTCA 
TTTAGAACGC CACAATCAAC CAAGACGCCA GCTGTCAGCT CCTTCAATGC AAACACTACC 
GCCACAGCTT CAACCCCCGC TACCACTCCT GTGAAGAAAA AGAGACAGTC CTGGTTCCCA 
CCACCCCCTC CTGGTCACAA TGCCTCTCCA AGCACAGCAG CCAGCAGAAG GGACCCAGCT 
CTGCACCCTC CACTCCCCCC AAAGCCCTGT TCTCCCATTG CTTCTCCTAA ACCATTGGGG 
CGGACTAATA GGCAGATACA TGCAGCTACA GCGGGGGCAT GTGGAGAAAC AGAACGACAT 
GCTGAAAGAA ATATAAGGAC TGAGGATCTC GATGACATCA TCAGGGTGGC GGCTGCACTT 
CAGAAAACTG ACAAGGGTGA AGAATTGGAT AATCTCATCA GAATGAACAA AAGCTTGAAC 
AGAAACCAAG GTCTCGATGG CCTCTTCCGA GCAAACCTGA AGGCACAGCA ATTGGACAAA 
AGAGCCCAGA GTCTTGAAAG CCTGATCTAT ATGAACACAC AGACGGACAG AGATGGCAAA 
GGAAATCAAG CCTTCGGGAG CCTTAAGAAA ATCAACCAAA GGGCTGACCC CGACAGAAGA 
AGCCAAGATC TCAGATCAGT TATCAGAACA CATGCCACGG CTGAGAGAAT CGGCAGAAGA 
AAACAAGACC TCGATGGACT GATTAAAGTG AATCCTGACA CAAATAAAAA TATGAAGAGG 
GGCAAGAGCC TTGACAATCT TATCAAAGTC ACCCCGGAAG TAAACAGAAG CAACAAAGGG 
GGTCCAAGTC TTGACAATTT CACCAAAGGA GTTCCTGCAA GGAGCAGAGC TAACCAAAGG 
GACCAAGACC TGGACAGTCT TATCAAGGTG ACCCCCTCAG CAAACAGAAG CAGCCAGCAC 
AGTCTTGATG AACTTATTAA TACAAGCCCC CAAACTATCA AAACTACTGC TAGACACCAA 
GATCTCGATA AGTTCATCAA AGTAAATCCT GATGTTCTCA CAAACAACCA AAGAAACCAT 
GATGTAGACA GCACCATCAG AGGGAATCCT ACAGGGACCA GATGCGAGCA AAGCGAAGAG 
TTGGACAATC TTATCAAAGT GAAGCCTTCT GCTCTCAGAA ACACAAATGG AGGCCAGGAC 
CTAGAAAGCT TAACTGAAGT AAATTCTCAT GTGGCTGAGA AGAATGGGAG GATAGATGGC 
CAAGCCAATG GATTGACTAA CAGTCTGTTC AAGGAGTCCA CAAGAGCCTC GGTCTATTCT 
TATGAAGCCA GAAACAGTCT CAGCTCCAAT TCTGGAAACA AGAATGGTGG ACCCAAGGAC 
ACTGTTGTTT ATACAAGGAC ATATGTGGAG AACAGTAAAT CACCAAAGGA TGGTTATCAG 
GAAAATATCT CAGGAAAATA TATACAAACC GTTTATTCAA CTTCAGATAG GTCTGTCATT 
GAAAGAGACA TGTGCACTTA CTGCCGCAAG CCCTTGGGGG TGGAAACCAA GATGATTTTA 
GATGAATTAC AGATCTGTTG CCATTCCACC TGCTTTAAGT GTGAAATATG CAAACGGCCT 
CTGGAAAATT TACAGGCAGG AGACAGTATT TGGATCTATA GGCAGACGAT ACACTGTGAG 
CCCTGCTACT CTAAAGTGAT GGCAAAATGG ATTCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Targeted deletion of the sciellin gene resulted in normal development and maturation.
Genesis (New York, N.Y. : 2000)42(4)219-28(2005 Aug)
Baden HP,Champliaud MF,Sundberg JP,Viel A

Evidence for a conserved function in synapse formation reveals Phr1 as a candidate gene for respiratory failure in newborn mice.
Molecular and cellular biology24(3)1096-105(2004 Feb)
Burgess RW,Peterson KA,Johnson MJ,Roix JJ,Welsh IC,O'Brien TP

Targeting a complex transcriptome: the construction of the mouse full-length cDNA encyclopedia.
Genome research13(6B)1273-89(2003 Jun)
Carninci P,Waki K,Shiraki T,Konno H,Shibata K,Itoh M,Aizawa K,Arakawa T,Ishii Y,Sasaki D,Bono H,Kondo S,Sugahara Y,Saito R,Osato N,Fukuda S,Sato K,Watahiki A,Hirozane-Kishikawa T,Nakamura M,Shibata Y,Yasunishi A,Kikuchi N,Yoshiki A,Kusakabe M,Gustincich S,Beisel K,Pavan W,Aidinis V,Nakagawara A,Held WA,Iwata H,Kono T,Nakauchi H,Lyons P,Wells C,Hume DA,Fagiolini M,Hensch TK,Brinkmeier M,Camper S,Hirota J,Mombaerts P,Muramatsu M,Okazaki Y,Kawai J,Hayashizaki Y

Functional and comparative genomic analysis of the piebald deletion region of mouse chromosome 14.
Genomics80(2)172-84(2002 Aug)
Peterson KA,King BL,Hagge-Greenberg A,Roix JJ,Bult CJ,O'Brien TP

Gene characterization of sciellin (SCEL) and protein localization in vertebrate epithelia displaying barrier properties.
Genomics70(2)264-8(2000 Dec)
Champliaud MF,Baden HP,Koch M,Jin W,Burgeson RE,Viel A

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Scel cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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