Lpin3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Lpin3
Entrez Gene ID	64899
Full Name	lipin 3
Synonyms	9130206L11Rik,AA438110,AV236139,mKIAA4023
General protein information	Preferred Names lipin 3 Names phosphatidate phosphatase LPIN3
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 H2

mRNA and Protein(s)

mRNA	Protein	Name
NM_022883.3	NP_075021.1	phosphatidate phosphatase LPIN3
XM_017319164.1	XP_017174653.1	phosphatidate phosphatase LPIN3 isoform X1
XM_017319164.2	XP_017174653.1	phosphatidate phosphatase LPIN3 isoform X1
NM_001199118.1	NP_001186047.1	phosphatidate phosphatase LPIN3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	LPIN3	XP_003316986.1
Canis lupus familiaris (dog)	LPIN3	XP_005635118.1
Bos taurus (cattle)	LPIN3	NP_001179469.1
Rattus norvegicus (Norway rat)	Lpin3	NP_001014206.1
Homo sapiens (human)	LPIN3	NP_075047.1
	LPIN3	XP_002808004.1
Mus musculus (house mouse)	Lpin3	NP_001186047.1

Gene Ontology
Bibliography

Related articles in PubMed

The phosphatidic acid-binding, polybasic domain is responsible for the differences in the phosphoregulation of lipins 1 and 3.
Boroda S, Takkellapati S, Lawrence RT, Entwisle SW, Pearson JM, Granade ME, Mullins GR, Eaton JM, Vill?n J, Harris TE
The Journal of biological chemistry292(50)20481-20493(2017 Dec)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Transcriptome Analysis of Targeted Mouse Mutations Reveals the Topography of Local Changes in Gene Expression.
West DB, Engelhard EK, Adkisson M, Nava AJ, Kirov JV, Cipollone A, Willis B, Rapp J, de Jong PJ, Lloyd KC
PLoS genetics12(2)e1005691(2016 Feb)

Lipin-1 and lipin-3 together determine adiposity in vivo.
Csaki LS, Dwyer JR, Li X, Nguyen MH, Dewald J, Brindley DN, Lusis AJ, Yoshinaga Y, de Jong P, Fong L, Young SG, Reue K
Molecular metabolism3(2)145-54(2014 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Lipin-3 exhibits phosphatidate phosphatase type-1 (PAP1) activity, which has a key role in glycerolipid synthesis.
Title: Three mammalian lipins act as phosphatidate phosphatases with distinct tissue expression patterns.

The following Lpin3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Lpin3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu02033	NM_001199118.1 Latest version!	Mus musculus lipin 3 (Lpin3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00
OMu02033	NM_022883.3 Latest version!	Mus musculus lipin 3 (Lpin3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00
OMu80365	XM_017319164.2 Latest version!	Mus musculus lipin 3 (Lpin3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu80365	XM_017319164.1	Mus musculus lipin 3 (Lpin3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Lpin3 ( NM_022883.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_075021.1 Mus musculus lipin 3 (Lpin3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu02033
Clone ID Related Accession (Same CDS sequence)	NM_022883.3 , NM_001199118.1
Accession Version	NM_022883.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2547bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-09
Organism	Mus musculus(house mouse)
Product	phosphatidate phosphatase LPIN3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK220228.1 and BB016248.1. On Nov 20, 2010 this sequence version replaced NM_022883.2. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein. ##Evidence-Data-START## Transcript exon combination :: AK220228.1, AF286724.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGAACTATG TAGGGCAGCT GGCAGAGACA GTGTTTGGAA CGGTTAAGGA GCTGTACAGG 
GGCCTGAACC CGGCCACACT GAGCGGCGGC ATCGATGTGC TGGTGGTAAG GCAGCGGGAC 
GGCTCCTTCC GATGCTCACC CTTCCACGTG CGCTTCGGCA AGCTGGGTGT CCTGCGGTCA 
CGGGAGAAGG TGGTGGACAT TGAGATCAAT GGTGAGCCCG TGGACCTGCA TATGAAACTG 
GGGGACAGCG GCGAGGCCTT CTTTGTCCAG GAGCTGGACA GTGATGAGGA AGATGTGCCT 
CCTCGTCTGT GCACCTCACC CATCCCATGG GGAGGCCTGT CGGGCTTTCC CTCAGACTCC 
CAGATAGGCA CAGCCAGTGA GCCTGAGGGC CTAGTCATTA CAGGGCGGAG AAAGAGGCGG 
CGAAGGAGGA AACCGAGGCG CAGGGAGGAG GATGCCGTGG ACTCCAGTTC TGAAGAGTTG 
GAGGCAGGTG CGGAGAGTGA GCTGACATTG CTGGAAAAGC CAACACCAGA GTCCCCAAGT 
GCTCAGGAGG CAGAGGAGCC CTCATCGCAG CCTAAGGACA TACACCCCTA CTCCGATGGC 
GAGTGTACCC CACAGGCCAA CCTCTCCTCT GGTGACCTAA TGTCCCCTAA GAGTGACTCA 
GAGCTAGAGC TGCGGTCCTT GGAGCCCAGT CCTCTGAGAG CTGAGTCTCA CATGCAGTGG 
GTCTGGGGCA GACTGCCTAA GGTGGCGAAA GCTGAACGGC CTGAGTTCTC GTTGATCCTT 
GAAAGTATGG CTGAGGCGAT CTGTGCTCTT CCTGAGGAGC CTAGCCCTTC CTCCTCTCCC 
TCTGAGGCTG GTGTGGACAC TTTGAGTCCC CCGGTGCTGC ACCCAGGAGT CAGGGCTGAT 
ACATTCCATC CTGCTGTGGA GGCCCATTGT GAGGAAACTG CAGTGGATTC TCCTCTTGCT 
GCCCCAGAGA GTAAGGAAAC CAAGACTCAG AACTCCAGGG GTGCAGGCCA CCCTCCTGCC 
ACTAAATCAT GGAGCTGGAC TACTCCAGAG AGCCATACTC CCTCTGGGCA TCCACAAGTT 
TCCAGGGGAA AGGGTTCCCT GAAGAGAAAC CAGCACTTGG GCCCAAGTGA CATCTACCTG 
GATGATCTAC CCTCCCTGGA CTCTGAGAAC GTAGCCCTTT ATTTCCCCAA GAGTGAGTAT 
GGGATGGGGG CCAGAAGGTG GAGTGAGCCC AGCAACCAGA AGCTCCTGGA GAGCCCCAAC 
CCTGAGCACA TAGCAGAATG CACTCTGGAC TCAGTGGACA AAATAGAATT GTCCCTTTGT 
GGTGGACTGG CTGACAACCG AGATATCTCC CTGGAGAAGT TCACCCAGCA CATGGTCTCC 
TATGAAGACC TCACTAAGAA TCCCGGGCTC CTGGATGACC CAAACCTCGT GGTGAAAATC 
AACGAGAAGC ATTACAACTG GGCTGTGGCT GCGCCCATGA TTCTTTCTCT GCAAGCCTTC 
CAGAAGAACT TACCTGAGAG CACTGTGGAC AAGCTGGAGA AGGAGAAGAT GCCCCGGAAG 
GGTGGGCGAT GGTGGTTTTC CTGGAGACGC AGGGACTTCC CAGCTGAGGA GCACAGTTCC 
CAGAGGGAGA AAGCCGCAAC CAGGAAACAG CAGGGAGAGA AGACAGAAGT CTTAAGCAGC 
GATGACGATG TCCCAGACAG CCCTGTGATC CTTGAAGTCC CCCCACTGCC ATCCTCCACT 
CCAGGCTATG TCCCGACCTA CAAGAAGTCT CTTCGTCTCT CTTCTGATCA GATTCGGTGT 
CTAAACCTGA ACGAAGGAGC TAACGATGTG GTCTTCAGTG TGACGACCCA GTACCAAGGC 
ACCTGCCGTT GCAAAGCCAC CATCTACCTG TGGAATTGGG ATGACAAGGT GGTCATCTCT 
GACATAGATG GCACCATCAC CAAGTCGGAT GCTCTGGGCC ACATTCTGCC CCAGCTGGGG 
AAAGACTGGA CACACCAGGG CATTACTAGT CTCTACCACA AGATCCATCT AAATGGGTAC 
AAGTTCCTGT ACTGCTCAGC ACGGGCTATC GGCATGGCTG ACCTCACCAA GGGGTACTTG 
CAGTGGGTGA GCGAGCATGG CTGTGGCCTT CCTAAGGGCC CCATCTTGCT ATCTCCCAGC 
AGCCTCTTCT CCGCTCTCCA CAGGGAGGTA ATCGAGAAAA AGCCGGAGGT GTTCAAGGTT 
GCCTGCCTGA GTGATATCCA GCAGCTGTTT CTGCCCCAAA GACAGCCCTT CCATGCTGCC 
TTTGGGAACA GGCCCAATGA TGTTTTCGCC TACAGGCAGG TGGGCCTCCC TGAGTCCCGA 
ATCTTCACAG TCAACCCCCG GGGGGAGCTC ATCCAGGAGC TCATAAAGAG CCACAAATCC 
ACGTACCAGC GGCTCGGAGA GGTGGTTGAG CTGCTCTTCC CACCTGTGGT GCGCGGTCCC 
AGCACAGACC TGGCCAGCCC TGAATACAGC AACCTCAGCT ATTGGCGGAA GCCACTGCCG 
TATGTGGACT TTGAGGCCCT GGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_075021.1
CDS	329..2875
Translation	MNYVGQLAETVFGTVKELYRGLNPATLSGGIDVLVVRQRDGSFRCSPFHVRFGKLGVLRSREKVVDIEINGEPVDLHMKLGDSGEAFFVQELDSDEEDVPPRLCTSPIPWGGLSGFPSDSQIGTASEPEGLVITGRRKRRRRRKPRRREEDAVDSSSEELEAGAESELTLLEKPTPESPSAQEAEEPSSQPKDIHPYSDGECTPQANLSSGDLMSPKSDSELELRSLEPSPLRAESHMQWVWGRLPKVAKAERPEFSLILESMAEAICALPEEPSPSSSPSEAGVDTLSPPVLHPGVRADTFHPAVEAHCEETAVDSPLAAPESKETKTQNSRGAGHPPATKSWSWTTPESHTPSGHPQVSRGKGSLKRNQHLGPSDIYLDDLPSLDSENVALYFPKSEYGMGARRWSEPSNQKLLESPNPEHIAECTLDSVDKIELSLCGGLADNRDISLEKFTQHMVSYEDLTKNPGLLDDPNLVVKINEKHYNWAVAAPMILSLQAFQKNLPESTVDKLEKEKMPRKGGRWWFSWRRRDFPAEEHSSQREKAATRKQQGEKTEVLSSDDDVPDSPVILEVPPLPSSTPGYVPTYKKSLRLSSDQIRCLNLNEGANDVVFSVTTQYQGTCRCKATIYLWNWDDKVVISDIDGTITKSDALGHILPQLGKDWTHQGITSLYHKIHLNGYKFLYCSARAIGMADLTKGYLQWVSEHGCGLPKGPILLSPSSLFSALHREVIEKKPEVFKVACLSDIQQLFLPQRQPFHAAFGNRPNDVFAYRQVGLPESRIFTVNPRGELIQELIKSHKSTYQRLGEVVELLFPPVVRGPSTDLASPEYSNLSYWRKPLPYVDFEALA

Target ORF information:

RefSeq Version	NM_022883.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus lipin 3 (Lpin3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022883.3

ORF Insert Sequence:

ATGAACTATG TAGGGCAGCT GGCAGAGACA GTGTTTGGAA CGGTTAAGGA GCTGTACAGG 
GGCCTGAACC CGGCCACACT GAGCGGCGGC ATCGATGTGC TGGTGGTAAG GCAGCGGGAC 
GGCTCCTTCC GATGCTCACC CTTCCACGTG CGCTTCGGCA AGCTGGGTGT CCTGCGGTCA 
CGGGAGAAGG TGGTGGACAT TGAGATCAAT GGTGAGCCCG TGGACCTGCA TATGAAACTG 
GGGGACAGCG GCGAGGCCTT CTTTGTCCAG GAGCTGGACA GTGATGAGGA AGATGTGCCT 
CCTCGTCTGT GCACCTCACC CATCCCATGG GGAGGCCTGT CGGGCTTTCC CTCAGACTCC 
CAGATAGGCA CAGCCAGTGA GCCTGAGGGC CTAGTCATTA CAGGGCGGAG AAAGAGGCGG 
CGAAGGAGGA AACCGAGGCG CAGGGAGGAG GATGCCGTGG ACTCCAGTTC TGAAGAGTTG 
GAGGCAGGTG CGGAGAGTGA GCTGACATTG CTGGAAAAGC CAACACCAGA GTCCCCAAGT 
GCTCAGGAGG CAGAGGAGCC CTCATCGCAG CCTAAGGACA TACACCCCTA CTCCGATGGC 
GAGTGTACCC CACAGGCCAA CCTCTCCTCT GGTGACCTAA TGTCCCCTAA GAGTGACTCA 
GAGCTAGAGC TGCGGTCCTT GGAGCCCAGT CCTCTGAGAG CTGAGTCTCA CATGCAGTGG 
GTCTGGGGCA GACTGCCTAA GGTGGCGAAA GCTGAACGGC CTGAGTTCTC GTTGATCCTT 
GAAAGTATGG CTGAGGCGAT CTGTGCTCTT CCTGAGGAGC CTAGCCCTTC CTCCTCTCCC 
TCTGAGGCTG GTGTGGACAC TTTGAGTCCC CCGGTGCTGC ACCCAGGAGT CAGGGCTGAT 
ACATTCCATC CTGCTGTGGA GGCCCATTGT GAGGAAACTG CAGTGGATTC TCCTCTTGCT 
GCCCCAGAGA GTAAGGAAAC CAAGACTCAG AACTCCAGGG GTGCAGGCCA CCCTCCTGCC 
ACTAAATCAT GGAGCTGGAC TACTCCAGAG AGCCATACTC CCTCTGGGCA TCCACAAGTT 
TCCAGGGGAA AGGGTTCCCT GAAGAGAAAC CAGCACTTGG GCCCAAGTGA CATCTACCTG 
GATGATCTAC CCTCCCTGGA CTCTGAGAAC GTAGCCCTTT ATTTCCCCAA GAGTGAGTAT 
GGGATGGGGG CCAGAAGGTG GAGTGAGCCC AGCAACCAGA AGCTCCTGGA GAGCCCCAAC 
CCTGAGCACA TAGCAGAATG CACTCTGGAC TCAGTGGACA AAATAGAATT GTCCCTTTGT 
GGTGGACTGG CTGACAACCG AGATATCTCC CTGGAGAAGT TCACCCAGCA CATGGTCTCC 
TATGAAGACC TCACTAAGAA TCCCGGGCTC CTGGATGACC CAAACCTCGT GGTGAAAATC 
AACGAGAAGC ATTACAACTG GGCTGTGGCT GCGCCCATGA TTCTTTCTCT GCAAGCCTTC 
CAGAAGAACT TACCTGAGAG CACTGTGGAC AAGCTGGAGA AGGAGAAGAT GCCCCGGAAG 
GGTGGGCGAT GGTGGTTTTC CTGGAGACGC AGGGACTTCC CAGCTGAGGA GCACAGTTCC 
CAGAGGGAGA AAGCCGCAAC CAGGAAACAG CAGGGAGAGA AGACAGAAGT CTTAAGCAGC 
GATGACGATG TCCCAGACAG CCCTGTGATC CTTGAAGTCC CCCCACTGCC ATCCTCCACT 
CCAGGCTATG TCCCGACCTA CAAGAAGTCT CTTCGTCTCT CTTCTGATCA GATTCGGTGT 
CTAAACCTGA ACGAAGGAGC TAACGATGTG GTCTTCAGTG TGACGACCCA GTACCAAGGC 
ACCTGCCGTT GCAAAGCCAC CATCTACCTG TGGAATTGGG ATGACAAGGT GGTCATCTCT 
GACATAGATG GCACCATCAC CAAGTCGGAT GCTCTGGGCC ACATTCTGCC CCAGCTGGGG 
AAAGACTGGA CACACCAGGG CATTACTAGT CTCTACCACA AGATCCATCT AAATGGGTAC 
AAGTTCCTGT ACTGCTCAGC ACGGGCTATC GGCATGGCTG ACCTCACCAA GGGGTACTTG 
CAGTGGGTGA GCGAGCATGG CTGTGGCCTT CCTAAGGGCC CCATCTTGCT ATCTCCCAGC 
AGCCTCTTCT CCGCTCTCCA CAGGGAGGTA ATCGAGAAAA AGCCGGAGGT GTTCAAGGTT 
GCCTGCCTGA GTGATATCCA GCAGCTGTTT CTGCCCCAAA GACAGCCCTT CCATGCTGCC 
TTTGGGAACA GGCCCAATGA TGTTTTCGCC TACAGGCAGG TGGGCCTCCC TGAGTCCCGA 
ATCTTCACAG TCAACCCCCG GGGGGAGCTC ATCCAGGAGC TCATAAAGAG CCACAAATCC 
ACGTACCAGC GGCTCGGAGA GGTGGTTGAG CTGCTCTTCC CACCTGTGGT GCGCGGTCCC 
AGCACAGACC TGGCCAGCCC TGAATACAGC AACCTCAGCT ATTGGCGGAA GCCACTGCCG 
TATGTGGACT TTGAGGCCCT GGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lpin3 ( NM_001199118.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001186047.1 Mus musculus lipin 3 (Lpin3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu02033
Clone ID Related Accession (Same CDS sequence)	NM_022883.3 , NM_001199118.1
Accession Version	NM_001199118.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2547bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	phosphatidate phosphatase LPIN3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB659464.1 and AK163833.1. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein. ##Evidence-Data-START## Transcript exon combination :: AK163833.1, AK049363.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGAACTATG TAGGGCAGCT GGCAGAGACA GTGTTTGGAA CGGTTAAGGA GCTGTACAGG 
GGCCTGAACC CGGCCACACT GAGCGGCGGC ATCGATGTGC TGGTGGTAAG GCAGCGGGAC 
GGCTCCTTCC GATGCTCACC CTTCCACGTG CGCTTCGGCA AGCTGGGTGT CCTGCGGTCA 
CGGGAGAAGG TGGTGGACAT TGAGATCAAT GGTGAGCCCG TGGACCTGCA TATGAAACTG 
GGGGACAGCG GCGAGGCCTT CTTTGTCCAG GAGCTGGACA GTGATGAGGA AGATGTGCCT 
CCTCGTCTGT GCACCTCACC CATCCCATGG GGAGGCCTGT CGGGCTTTCC CTCAGACTCC 
CAGATAGGCA CAGCCAGTGA GCCTGAGGGC CTAGTCATTA CAGGGCGGAG AAAGAGGCGG 
CGAAGGAGGA AACCGAGGCG CAGGGAGGAG GATGCCGTGG ACTCCAGTTC TGAAGAGTTG 
GAGGCAGGTG CGGAGAGTGA GCTGACATTG CTGGAAAAGC CAACACCAGA GTCCCCAAGT 
GCTCAGGAGG CAGAGGAGCC CTCATCGCAG CCTAAGGACA TACACCCCTA CTCCGATGGC 
GAGTGTACCC CACAGGCCAA CCTCTCCTCT GGTGACCTAA TGTCCCCTAA GAGTGACTCA 
GAGCTAGAGC TGCGGTCCTT GGAGCCCAGT CCTCTGAGAG CTGAGTCTCA CATGCAGTGG 
GTCTGGGGCA GACTGCCTAA GGTGGCGAAA GCTGAACGGC CTGAGTTCTC GTTGATCCTT 
GAAAGTATGG CTGAGGCGAT CTGTGCTCTT CCTGAGGAGC CTAGCCCTTC CTCCTCTCCC 
TCTGAGGCTG GTGTGGACAC TTTGAGTCCC CCGGTGCTGC ACCCAGGAGT CAGGGCTGAT 
ACATTCCATC CTGCTGTGGA GGCCCATTGT GAGGAAACTG CAGTGGATTC TCCTCTTGCT 
GCCCCAGAGA GTAAGGAAAC CAAGACTCAG AACTCCAGGG GTGCAGGCCA CCCTCCTGCC 
ACTAAATCAT GGAGCTGGAC TACTCCAGAG AGCCATACTC CCTCTGGGCA TCCACAAGTT 
TCCAGGGGAA AGGGTTCCCT GAAGAGAAAC CAGCACTTGG GCCCAAGTGA CATCTACCTG 
GATGATCTAC CCTCCCTGGA CTCTGAGAAC GTAGCCCTTT ATTTCCCCAA GAGTGAGTAT 
GGGATGGGGG CCAGAAGGTG GAGTGAGCCC AGCAACCAGA AGCTCCTGGA GAGCCCCAAC 
CCTGAGCACA TAGCAGAATG CACTCTGGAC TCAGTGGACA AAATAGAATT GTCCCTTTGT 
GGTGGACTGG CTGACAACCG AGATATCTCC CTGGAGAAGT TCACCCAGCA CATGGTCTCC 
TATGAAGACC TCACTAAGAA TCCCGGGCTC CTGGATGACC CAAACCTCGT GGTGAAAATC 
AACGAGAAGC ATTACAACTG GGCTGTGGCT GCGCCCATGA TTCTTTCTCT GCAAGCCTTC 
CAGAAGAACT TACCTGAGAG CACTGTGGAC AAGCTGGAGA AGGAGAAGAT GCCCCGGAAG 
GGTGGGCGAT GGTGGTTTTC CTGGAGACGC AGGGACTTCC CAGCTGAGGA GCACAGTTCC 
CAGAGGGAGA AAGCCGCAAC CAGGAAACAG CAGGGAGAGA AGACAGAAGT CTTAAGCAGC 
GATGACGATG TCCCAGACAG CCCTGTGATC CTTGAAGTCC CCCCACTGCC ATCCTCCACT 
CCAGGCTATG TCCCGACCTA CAAGAAGTCT CTTCGTCTCT CTTCTGATCA GATTCGGTGT 
CTAAACCTGA ACGAAGGAGC TAACGATGTG GTCTTCAGTG TGACGACCCA GTACCAAGGC 
ACCTGCCGTT GCAAAGCCAC CATCTACCTG TGGAATTGGG ATGACAAGGT GGTCATCTCT 
GACATAGATG GCACCATCAC CAAGTCGGAT GCTCTGGGCC ACATTCTGCC CCAGCTGGGG 
AAAGACTGGA CACACCAGGG CATTACTAGT CTCTACCACA AGATCCATCT AAATGGGTAC 
AAGTTCCTGT ACTGCTCAGC ACGGGCTATC GGCATGGCTG ACCTCACCAA GGGGTACTTG 
CAGTGGGTGA GCGAGCATGG CTGTGGCCTT CCTAAGGGCC CCATCTTGCT ATCTCCCAGC 
AGCCTCTTCT CCGCTCTCCA CAGGGAGGTA ATCGAGAAAA AGCCGGAGGT GTTCAAGGTT 
GCCTGCCTGA GTGATATCCA GCAGCTGTTT CTGCCCCAAA GACAGCCCTT CCATGCTGCC 
TTTGGGAACA GGCCCAATGA TGTTTTCGCC TACAGGCAGG TGGGCCTCCC TGAGTCCCGA 
ATCTTCACAG TCAACCCCCG GGGGGAGCTC ATCCAGGAGC TCATAAAGAG CCACAAATCC 
ACGTACCAGC GGCTCGGAGA GGTGGTTGAG CTGCTCTTCC CACCTGTGGT GCGCGGTCCC 
AGCACAGACC TGGCCAGCCC TGAATACAGC AACCTCAGCT ATTGGCGGAA GCCACTGCCG 
TATGTGGACT TTGAGGCCCT GGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001186047.1
CDS	252..2798
Translation	MNYVGQLAETVFGTVKELYRGLNPATLSGGIDVLVVRQRDGSFRCSPFHVRFGKLGVLRSREKVVDIEINGEPVDLHMKLGDSGEAFFVQELDSDEEDVPPRLCTSPIPWGGLSGFPSDSQIGTASEPEGLVITGRRKRRRRRKPRRREEDAVDSSSEELEAGAESELTLLEKPTPESPSAQEAEEPSSQPKDIHPYSDGECTPQANLSSGDLMSPKSDSELELRSLEPSPLRAESHMQWVWGRLPKVAKAERPEFSLILESMAEAICALPEEPSPSSSPSEAGVDTLSPPVLHPGVRADTFHPAVEAHCEETAVDSPLAAPESKETKTQNSRGAGHPPATKSWSWTTPESHTPSGHPQVSRGKGSLKRNQHLGPSDIYLDDLPSLDSENVALYFPKSEYGMGARRWSEPSNQKLLESPNPEHIAECTLDSVDKIELSLCGGLADNRDISLEKFTQHMVSYEDLTKNPGLLDDPNLVVKINEKHYNWAVAAPMILSLQAFQKNLPESTVDKLEKEKMPRKGGRWWFSWRRRDFPAEEHSSQREKAATRKQQGEKTEVLSSDDDVPDSPVILEVPPLPSSTPGYVPTYKKSLRLSSDQIRCLNLNEGANDVVFSVTTQYQGTCRCKATIYLWNWDDKVVISDIDGTITKSDALGHILPQLGKDWTHQGITSLYHKIHLNGYKFLYCSARAIGMADLTKGYLQWVSEHGCGLPKGPILLSPSSLFSALHREVIEKKPEVFKVACLSDIQQLFLPQRQPFHAAFGNRPNDVFAYRQVGLPESRIFTVNPRGELIQELIKSHKSTYQRLGEVVELLFPPVVRGPSTDLASPEYSNLSYWRKPLPYVDFEALA

Target ORF information:

RefSeq Version	NM_001199118.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus lipin 3 (Lpin3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001199118.1

ORF Insert Sequence:

ATGAACTATG TAGGGCAGCT GGCAGAGACA GTGTTTGGAA CGGTTAAGGA GCTGTACAGG 
GGCCTGAACC CGGCCACACT GAGCGGCGGC ATCGATGTGC TGGTGGTAAG GCAGCGGGAC 
GGCTCCTTCC GATGCTCACC CTTCCACGTG CGCTTCGGCA AGCTGGGTGT CCTGCGGTCA 
CGGGAGAAGG TGGTGGACAT TGAGATCAAT GGTGAGCCCG TGGACCTGCA TATGAAACTG 
GGGGACAGCG GCGAGGCCTT CTTTGTCCAG GAGCTGGACA GTGATGAGGA AGATGTGCCT 
CCTCGTCTGT GCACCTCACC CATCCCATGG GGAGGCCTGT CGGGCTTTCC CTCAGACTCC 
CAGATAGGCA CAGCCAGTGA GCCTGAGGGC CTAGTCATTA CAGGGCGGAG AAAGAGGCGG 
CGAAGGAGGA AACCGAGGCG CAGGGAGGAG GATGCCGTGG ACTCCAGTTC TGAAGAGTTG 
GAGGCAGGTG CGGAGAGTGA GCTGACATTG CTGGAAAAGC CAACACCAGA GTCCCCAAGT 
GCTCAGGAGG CAGAGGAGCC CTCATCGCAG CCTAAGGACA TACACCCCTA CTCCGATGGC 
GAGTGTACCC CACAGGCCAA CCTCTCCTCT GGTGACCTAA TGTCCCCTAA GAGTGACTCA 
GAGCTAGAGC TGCGGTCCTT GGAGCCCAGT CCTCTGAGAG CTGAGTCTCA CATGCAGTGG 
GTCTGGGGCA GACTGCCTAA GGTGGCGAAA GCTGAACGGC CTGAGTTCTC GTTGATCCTT 
GAAAGTATGG CTGAGGCGAT CTGTGCTCTT CCTGAGGAGC CTAGCCCTTC CTCCTCTCCC 
TCTGAGGCTG GTGTGGACAC TTTGAGTCCC CCGGTGCTGC ACCCAGGAGT CAGGGCTGAT 
ACATTCCATC CTGCTGTGGA GGCCCATTGT GAGGAAACTG CAGTGGATTC TCCTCTTGCT 
GCCCCAGAGA GTAAGGAAAC CAAGACTCAG AACTCCAGGG GTGCAGGCCA CCCTCCTGCC 
ACTAAATCAT GGAGCTGGAC TACTCCAGAG AGCCATACTC CCTCTGGGCA TCCACAAGTT 
TCCAGGGGAA AGGGTTCCCT GAAGAGAAAC CAGCACTTGG GCCCAAGTGA CATCTACCTG 
GATGATCTAC CCTCCCTGGA CTCTGAGAAC GTAGCCCTTT ATTTCCCCAA GAGTGAGTAT 
GGGATGGGGG CCAGAAGGTG GAGTGAGCCC AGCAACCAGA AGCTCCTGGA GAGCCCCAAC 
CCTGAGCACA TAGCAGAATG CACTCTGGAC TCAGTGGACA AAATAGAATT GTCCCTTTGT 
GGTGGACTGG CTGACAACCG AGATATCTCC CTGGAGAAGT TCACCCAGCA CATGGTCTCC 
TATGAAGACC TCACTAAGAA TCCCGGGCTC CTGGATGACC CAAACCTCGT GGTGAAAATC 
AACGAGAAGC ATTACAACTG GGCTGTGGCT GCGCCCATGA TTCTTTCTCT GCAAGCCTTC 
CAGAAGAACT TACCTGAGAG CACTGTGGAC AAGCTGGAGA AGGAGAAGAT GCCCCGGAAG 
GGTGGGCGAT GGTGGTTTTC CTGGAGACGC AGGGACTTCC CAGCTGAGGA GCACAGTTCC 
CAGAGGGAGA AAGCCGCAAC CAGGAAACAG CAGGGAGAGA AGACAGAAGT CTTAAGCAGC 
GATGACGATG TCCCAGACAG CCCTGTGATC CTTGAAGTCC CCCCACTGCC ATCCTCCACT 
CCAGGCTATG TCCCGACCTA CAAGAAGTCT CTTCGTCTCT CTTCTGATCA GATTCGGTGT 
CTAAACCTGA ACGAAGGAGC TAACGATGTG GTCTTCAGTG TGACGACCCA GTACCAAGGC 
ACCTGCCGTT GCAAAGCCAC CATCTACCTG TGGAATTGGG ATGACAAGGT GGTCATCTCT 
GACATAGATG GCACCATCAC CAAGTCGGAT GCTCTGGGCC ACATTCTGCC CCAGCTGGGG 
AAAGACTGGA CACACCAGGG CATTACTAGT CTCTACCACA AGATCCATCT AAATGGGTAC 
AAGTTCCTGT ACTGCTCAGC ACGGGCTATC GGCATGGCTG ACCTCACCAA GGGGTACTTG 
CAGTGGGTGA GCGAGCATGG CTGTGGCCTT CCTAAGGGCC CCATCTTGCT ATCTCCCAGC 
AGCCTCTTCT CCGCTCTCCA CAGGGAGGTA ATCGAGAAAA AGCCGGAGGT GTTCAAGGTT 
GCCTGCCTGA GTGATATCCA GCAGCTGTTT CTGCCCCAAA GACAGCCCTT CCATGCTGCC 
TTTGGGAACA GGCCCAATGA TGTTTTCGCC TACAGGCAGG TGGGCCTCCC TGAGTCCCGA 
ATCTTCACAG TCAACCCCCG GGGGGAGCTC ATCCAGGAGC TCATAAAGAG CCACAAATCC 
ACGTACCAGC GGCTCGGAGA GGTGGTTGAG CTGCTCTTCC CACCTGTGGT GCGCGGTCCC 
AGCACAGACC TGGCCAGCCC TGAATACAGC AACCTCAGCT ATTGGCGGAA GCCACTGCCG 
TATGTGGACT TTGAGGCCCT GGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lpin3 ( XM_017319164.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017174653.1 Mus musculus lipin 3 (Lpin3), transcript variant X3, mRNA.

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CloneID	OMu80365
Clone ID Related Accession (Same CDS sequence)	XM_017319164.1 , XM_017319164.2
Accession Version	XM_017319164.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1554bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	phosphatidate phosphatase LPIN3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTATG TAGGGCAGCT GGCAGAGACA GTGTTTGGAA CGGTTAAGGA GCTGTACAGG 
GGCCTGAACC CGGCCACACT GAGCGGCGGC ATCGATGTGC TGGTGGTAAG GCAGCGGGAC 
GGCTCCTTCC GATGCTCACC CTTCCACGTG CGCTTCGGCA AGCTGGGTGT CCTGCGGTCA 
CGGGAGAAGG TGGTGGACAT TGAGATCAAT GGTGAGCCCG TGGACCTGCA TATGAAACTG 
GGGGACAGCG GCGAGGCCTT CTTTGTCCAG GAGCTGGACA GTGATGAGGA AGATGTGCCT 
CCTCGTCTGT GCACCTCACC CATCCCATGG GGAGGCCTGT CGGGCTTTCC CTCAGACTCC 
CAGATAGGCA CAGCCAGTGA GCCTGAGGGC CTAGTCATTA CAGGGCGGAG AAAGAGGCGG 
CGAAGGAGGA AACCGAGGCG CAGGGAGGAG GATGCCGTGG ACTCCAGTTC TGAAGAGTTG 
GAGGCAGGTG CGGAGAGTGA GCTGACATTG CTGGAAAAGC CAACACCAGA GTCCCCAAGT 
GCTCAGGAGG CAGAGGAGCC CTCATCGCAG CCTAAGGACA TACACCCCTA CTCCGATGGC 
GAGTGTACCC CACAGGCCAA CCTCTCCTCT GGTGACCTAA TGTCCCCTAA GAGTGACTCA 
GAGCTAGAGC TGCGGTCCTT GGAGCCCAGT CCTCTGAGAG CTGAGTCTCA CATGCAGTGG 
GTCTGGGGCA GACTGCCTAA GGTGGCGAAA GCTGAACGGC CTGAGTTCTC GTTGATCCTT 
GAAAGTATGG CTGAGGCGAT CTGTGCTCTT CCTGAGGAGC CTAGCCCTTC CTCCTCTCCC 
TCTGAGGCTG GTGTGGACAC TTTGAGTCCC CCGGTGCTGC ACCCAGGAGT CAGGGCTGAT 
ACATTCCATC CTGCTGTGGA GGCCCATTGT GAGGAAACTG CAGTGGATTC TCCTCTTGCT 
GCCCCAGAGA GTAAGGAAAC CAAGACTCAG AACTCCAGGG GTGCAGGCCA CCCTCCTGCC 
ACTAAATCAT GGAGCTGGAC TACTCCAGAG AGCCATACTC CCTCTGGGCA TCCACAAGTT 
TCCAGGGGAA AGGGTTCCCT GAAGAGAAAC CAGCACTTGG GCCCAAGTGA CATCTACCTG 
GATGATCTAC CCTCCCTGGA CTCTGAGAAC GTAGCCCTTT ATTTCCCCAA GAGTGAGTAT 
GGGATGGGGG CCAGAAGGTG GAGTGAGCCC AGCAACCAGA AGCTCCTGGA GAGCCCCAAC 
CCTGAGCACA TAGCAGAATG CACTCTGGAC TCAGTGGACA AAATAGAATT GTCCCTTTGT 
GGTGGACTGG CTGACAACCG AGATATCTCC CTGGAGAAGT TCACCCAGCA CATGGTCTCC 
TATGAAGACC TCACTAAGAA TCCCGGGCTC CTGGATGACC CAAACCTCGT GGTGAAAATC 
AACGAGAAGC ATTACAACTG GGCTGTGGCT GCGCCCATGA TTCTTTCTCT GCAAGCCTTC 
CAGAAGAACT TACCTGAGGT AATGCTTAGA CCACCACTGG GTCCGATGCC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017174653.1
CDS	337..1890
Translation	MNYVGQLAETVFGTVKELYRGLNPATLSGGIDVLVVRQRDGSFRCSPFHVRFGKLGVLRSREKVVDIEINGEPVDLHMKLGDSGEAFFVQELDSDEEDVPPRLCTSPIPWGGLSGFPSDSQIGTASEPEGLVITGRRKRRRRRKPRRREEDAVDSSSEELEAGAESELTLLEKPTPESPSAQEAEEPSSQPKDIHPYSDGECTPQANLSSGDLMSPKSDSELELRSLEPSPLRAESHMQWVWGRLPKVAKAERPEFSLILESMAEAICALPEEPSPSSSPSEAGVDTLSPPVLHPGVRADTFHPAVEAHCEETAVDSPLAAPESKETKTQNSRGAGHPPATKSWSWTTPESHTPSGHPQVSRGKGSLKRNQHLGPSDIYLDDLPSLDSENVALYFPKSEYGMGARRWSEPSNQKLLESPNPEHIAECTLDSVDKIELSLCGGLADNRDISLEKFTQHMVSYEDLTKNPGLLDDPNLVVKINEKHYNWAVAAPMILSLQAFQKNLPEVMLRPPLGPMP

Target ORF information:

RefSeq Version	XM_017319164.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus lipin 3 (Lpin3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319164.1

ORF Insert Sequence:

ATGAACTATG TAGGGCAGCT GGCAGAGACA GTGTTTGGAA CGGTTAAGGA GCTGTACAGG 
GGCCTGAACC CGGCCACACT GAGCGGCGGC ATCGATGTGC TGGTGGTAAG GCAGCGGGAC 
GGCTCCTTCC GATGCTCACC CTTCCACGTG CGCTTCGGCA AGCTGGGTGT CCTGCGGTCA 
CGGGAGAAGG TGGTGGACAT TGAGATCAAT GGTGAGCCCG TGGACCTGCA TATGAAACTG 
GGGGACAGCG GCGAGGCCTT CTTTGTCCAG GAGCTGGACA GTGATGAGGA AGATGTGCCT 
CCTCGTCTGT GCACCTCACC CATCCCATGG GGAGGCCTGT CGGGCTTTCC CTCAGACTCC 
CAGATAGGCA CAGCCAGTGA GCCTGAGGGC CTAGTCATTA CAGGGCGGAG AAAGAGGCGG 
CGAAGGAGGA AACCGAGGCG CAGGGAGGAG GATGCCGTGG ACTCCAGTTC TGAAGAGTTG 
GAGGCAGGTG CGGAGAGTGA GCTGACATTG CTGGAAAAGC CAACACCAGA GTCCCCAAGT 
GCTCAGGAGG CAGAGGAGCC CTCATCGCAG CCTAAGGACA TACACCCCTA CTCCGATGGC 
GAGTGTACCC CACAGGCCAA CCTCTCCTCT GGTGACCTAA TGTCCCCTAA GAGTGACTCA 
GAGCTAGAGC TGCGGTCCTT GGAGCCCAGT CCTCTGAGAG CTGAGTCTCA CATGCAGTGG 
GTCTGGGGCA GACTGCCTAA GGTGGCGAAA GCTGAACGGC CTGAGTTCTC GTTGATCCTT 
GAAAGTATGG CTGAGGCGAT CTGTGCTCTT CCTGAGGAGC CTAGCCCTTC CTCCTCTCCC 
TCTGAGGCTG GTGTGGACAC TTTGAGTCCC CCGGTGCTGC ACCCAGGAGT CAGGGCTGAT 
ACATTCCATC CTGCTGTGGA GGCCCATTGT GAGGAAACTG CAGTGGATTC TCCTCTTGCT 
GCCCCAGAGA GTAAGGAAAC CAAGACTCAG AACTCCAGGG GTGCAGGCCA CCCTCCTGCC 
ACTAAATCAT GGAGCTGGAC TACTCCAGAG AGCCATACTC CCTCTGGGCA TCCACAAGTT 
TCCAGGGGAA AGGGTTCCCT GAAGAGAAAC CAGCACTTGG GCCCAAGTGA CATCTACCTG 
GATGATCTAC CCTCCCTGGA CTCTGAGAAC GTAGCCCTTT ATTTCCCCAA GAGTGAGTAT 
GGGATGGGGG CCAGAAGGTG GAGTGAGCCC AGCAACCAGA AGCTCCTGGA GAGCCCCAAC 
CCTGAGCACA TAGCAGAATG CACTCTGGAC TCAGTGGACA AAATAGAATT GTCCCTTTGT 
GGTGGACTGG CTGACAACCG AGATATCTCC CTGGAGAAGT TCACCCAGCA CATGGTCTCC 
TATGAAGACC TCACTAAGAA TCCCGGGCTC CTGGATGACC CAAACCTCGT GGTGAAAATC 
AACGAGAAGC ATTACAACTG GGCTGTGGCT GCGCCCATGA TTCTTTCTCT GCAAGCCTTC 
CAGAAGAACT TACCTGAGGT AATGCTTAGA CCACCACTGG GTCCGATGCC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lpin3 ( XM_017319164.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017174653.1 Mus musculus lipin 3 (Lpin3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu80365
Clone ID Related Accession (Same CDS sequence)	XM_017319164.1 , XM_017319164.2
Accession Version	XM_017319164.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1554bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	phosphatidate phosphatase LPIN3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017319164.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTATG TAGGGCAGCT GGCAGAGACA GTGTTTGGAA CGGTTAAGGA GCTGTACAGG 
GGCCTGAACC CGGCCACACT GAGCGGCGGC ATCGATGTGC TGGTGGTAAG GCAGCGGGAC 
GGCTCCTTCC GATGCTCACC CTTCCACGTG CGCTTCGGCA AGCTGGGTGT CCTGCGGTCA 
CGGGAGAAGG TGGTGGACAT TGAGATCAAT GGTGAGCCCG TGGACCTGCA TATGAAACTG 
GGGGACAGCG GCGAGGCCTT CTTTGTCCAG GAGCTGGACA GTGATGAGGA AGATGTGCCT 
CCTCGTCTGT GCACCTCACC CATCCCATGG GGAGGCCTGT CGGGCTTTCC CTCAGACTCC 
CAGATAGGCA CAGCCAGTGA GCCTGAGGGC CTAGTCATTA CAGGGCGGAG AAAGAGGCGG 
CGAAGGAGGA AACCGAGGCG CAGGGAGGAG GATGCCGTGG ACTCCAGTTC TGAAGAGTTG 
GAGGCAGGTG CGGAGAGTGA GCTGACATTG CTGGAAAAGC CAACACCAGA GTCCCCAAGT 
GCTCAGGAGG CAGAGGAGCC CTCATCGCAG CCTAAGGACA TACACCCCTA CTCCGATGGC 
GAGTGTACCC CACAGGCCAA CCTCTCCTCT GGTGACCTAA TGTCCCCTAA GAGTGACTCA 
GAGCTAGAGC TGCGGTCCTT GGAGCCCAGT CCTCTGAGAG CTGAGTCTCA CATGCAGTGG 
GTCTGGGGCA GACTGCCTAA GGTGGCGAAA GCTGAACGGC CTGAGTTCTC GTTGATCCTT 
GAAAGTATGG CTGAGGCGAT CTGTGCTCTT CCTGAGGAGC CTAGCCCTTC CTCCTCTCCC 
TCTGAGGCTG GTGTGGACAC TTTGAGTCCC CCGGTGCTGC ACCCAGGAGT CAGGGCTGAT 
ACATTCCATC CTGCTGTGGA GGCCCATTGT GAGGAAACTG CAGTGGATTC TCCTCTTGCT 
GCCCCAGAGA GTAAGGAAAC CAAGACTCAG AACTCCAGGG GTGCAGGCCA CCCTCCTGCC 
ACTAAATCAT GGAGCTGGAC TACTCCAGAG AGCCATACTC CCTCTGGGCA TCCACAAGTT 
TCCAGGGGAA AGGGTTCCCT GAAGAGAAAC CAGCACTTGG GCCCAAGTGA CATCTACCTG 
GATGATCTAC CCTCCCTGGA CTCTGAGAAC GTAGCCCTTT ATTTCCCCAA GAGTGAGTAT 
GGGATGGGGG CCAGAAGGTG GAGTGAGCCC AGCAACCAGA AGCTCCTGGA GAGCCCCAAC 
CCTGAGCACA TAGCAGAATG CACTCTGGAC TCAGTGGACA AAATAGAATT GTCCCTTTGT 
GGTGGACTGG CTGACAACCG AGATATCTCC CTGGAGAAGT TCACCCAGCA CATGGTCTCC 
TATGAAGACC TCACTAAGAA TCCCGGGCTC CTGGATGACC CAAACCTCGT GGTGAAAATC 
AACGAGAAGC ATTACAACTG GGCTGTGGCT GCGCCCATGA TTCTTTCTCT GCAAGCCTTC 
CAGAAGAACT TACCTGAGGT AATGCTTAGA CCACCACTGG GTCCGATGCC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017174653.1
CDS	337..1890
Translation	MNYVGQLAETVFGTVKELYRGLNPATLSGGIDVLVVRQRDGSFRCSPFHVRFGKLGVLRSREKVVDIEINGEPVDLHMKLGDSGEAFFVQELDSDEEDVPPRLCTSPIPWGGLSGFPSDSQIGTASEPEGLVITGRRKRRRRRKPRRREEDAVDSSSEELEAGAESELTLLEKPTPESPSAQEAEEPSSQPKDIHPYSDGECTPQANLSSGDLMSPKSDSELELRSLEPSPLRAESHMQWVWGRLPKVAKAERPEFSLILESMAEAICALPEEPSPSSSPSEAGVDTLSPPVLHPGVRADTFHPAVEAHCEETAVDSPLAAPESKETKTQNSRGAGHPPATKSWSWTTPESHTPSGHPQVSRGKGSLKRNQHLGPSDIYLDDLPSLDSENVALYFPKSEYGMGARRWSEPSNQKLLESPNPEHIAECTLDSVDKIELSLCGGLADNRDISLEKFTQHMVSYEDLTKNPGLLDDPNLVVKINEKHYNWAVAAPMILSLQAFQKNLPEVMLRPPLGPMP

Target ORF information:

RefSeq Version	XM_017319164.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus lipin 3 (Lpin3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319164.2

ORF Insert Sequence:

ATGAACTATG TAGGGCAGCT GGCAGAGACA GTGTTTGGAA CGGTTAAGGA GCTGTACAGG 
GGCCTGAACC CGGCCACACT GAGCGGCGGC ATCGATGTGC TGGTGGTAAG GCAGCGGGAC 
GGCTCCTTCC GATGCTCACC CTTCCACGTG CGCTTCGGCA AGCTGGGTGT CCTGCGGTCA 
CGGGAGAAGG TGGTGGACAT TGAGATCAAT GGTGAGCCCG TGGACCTGCA TATGAAACTG 
GGGGACAGCG GCGAGGCCTT CTTTGTCCAG GAGCTGGACA GTGATGAGGA AGATGTGCCT 
CCTCGTCTGT GCACCTCACC CATCCCATGG GGAGGCCTGT CGGGCTTTCC CTCAGACTCC 
CAGATAGGCA CAGCCAGTGA GCCTGAGGGC CTAGTCATTA CAGGGCGGAG AAAGAGGCGG 
CGAAGGAGGA AACCGAGGCG CAGGGAGGAG GATGCCGTGG ACTCCAGTTC TGAAGAGTTG 
GAGGCAGGTG CGGAGAGTGA GCTGACATTG CTGGAAAAGC CAACACCAGA GTCCCCAAGT 
GCTCAGGAGG CAGAGGAGCC CTCATCGCAG CCTAAGGACA TACACCCCTA CTCCGATGGC 
GAGTGTACCC CACAGGCCAA CCTCTCCTCT GGTGACCTAA TGTCCCCTAA GAGTGACTCA 
GAGCTAGAGC TGCGGTCCTT GGAGCCCAGT CCTCTGAGAG CTGAGTCTCA CATGCAGTGG 
GTCTGGGGCA GACTGCCTAA GGTGGCGAAA GCTGAACGGC CTGAGTTCTC GTTGATCCTT 
GAAAGTATGG CTGAGGCGAT CTGTGCTCTT CCTGAGGAGC CTAGCCCTTC CTCCTCTCCC 
TCTGAGGCTG GTGTGGACAC TTTGAGTCCC CCGGTGCTGC ACCCAGGAGT CAGGGCTGAT 
ACATTCCATC CTGCTGTGGA GGCCCATTGT GAGGAAACTG CAGTGGATTC TCCTCTTGCT 
GCCCCAGAGA GTAAGGAAAC CAAGACTCAG AACTCCAGGG GTGCAGGCCA CCCTCCTGCC 
ACTAAATCAT GGAGCTGGAC TACTCCAGAG AGCCATACTC CCTCTGGGCA TCCACAAGTT 
TCCAGGGGAA AGGGTTCCCT GAAGAGAAAC CAGCACTTGG GCCCAAGTGA CATCTACCTG 
GATGATCTAC CCTCCCTGGA CTCTGAGAAC GTAGCCCTTT ATTTCCCCAA GAGTGAGTAT 
GGGATGGGGG CCAGAAGGTG GAGTGAGCCC AGCAACCAGA AGCTCCTGGA GAGCCCCAAC 
CCTGAGCACA TAGCAGAATG CACTCTGGAC TCAGTGGACA AAATAGAATT GTCCCTTTGT 
GGTGGACTGG CTGACAACCG AGATATCTCC CTGGAGAAGT TCACCCAGCA CATGGTCTCC 
TATGAAGACC TCACTAAGAA TCCCGGGCTC CTGGATGACC CAAACCTCGT GGTGAAAATC 
AACGAGAAGC ATTACAACTG GGCTGTGGCT GCGCCCATGA TTCTTTCTCT GCAAGCCTTC 
CAGAAGAACT TACCTGAGGT AATGCTTAGA CCACCACTGG GTCCGATGCC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The phosphatidic acid-binding, polybasic domain is responsible for the differences in the phosphoregulation of lipins 1 and 3.
The Journal of biological chemistry292(50)20481-20493(2017 Dec)
Boroda S,Takkellapati S,Lawrence RT,Entwisle SW,Pearson JM,Granade ME,Mullins GR,Eaton JM,Vill?n J,Harris TE

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Transcriptome Analysis of Targeted Mouse Mutations Reveals the Topography of Local Changes in Gene Expression.
PLoS genetics12(2)e1005691(2016 Feb)
West DB,Engelhard EK,Adkisson M,Nava AJ,Kirov JV,Cipollone A,Willis B,Rapp J,de Jong PJ,Lloyd KC

Lipin-1 and lipin-3 together determine adiposity in vivo.
Molecular metabolism3(2)145-54(2014 Apr)
Csaki LS,Dwyer JR,Li X,Nguyen MH,Dewald J,Brindley DN,Lusis AJ,Yoshinaga Y,de Jong P,Fong L,Young SG,Reue K

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Lpin3 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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