Bco1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Bco1
Entrez Gene ID	63857
Full Name	beta-carotene oxygenase 1
Synonyms	Bcdo,Bcdo1,Bcmo1,CMO1,Cmoi,beta-CD,betaCMOOX
General protein information	Preferred Names beta-carotene oxygenase 1 Names beta,beta-carotene 15,15'-dioxygenase beta,beta-carotene 15,15'-monooxygenase beta-carotene 15, 15'-dioxygenase 1 beta-carotene 15,15' dioxygenase beta-carotene 15,15'-monooxygenase beta-carotene cleavage enzyme beta-carotene dioxygenase 1 carotenoid-15,15'-oxygenase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8\|8 E1
Summary	Vitamin A metabolism is important for vital processes such as vision, embryonic development, cell differentiation, and membrane and skin protection. The protein encoded by this gene is a key enzyme in beta-carotene metabolism to vitamin A. It catalyzes the oxidative cleavage of beta,beta-carotene into two retinal molecules. Two alternatively spliced variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2009].

mRNA and Protein(s)

mRNA	Protein	Name
XM_011248481.2	XP_011246783.1	beta,beta-carotene 15,15'-dioxygenase isoform X1
NM_001163028.1	NP_001156500.1	beta,beta-carotene 15,15'-dioxygenase isoform 2
NM_021486.3	NP_067461.2	beta,beta-carotene 15,15'-dioxygenase isoform 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	BCMO1	NP_059125.2
Canis lupus familiaris (dog)	BCMO1	XP_546815.1
Gallus gallus (chicken)	BCMO1	NP_989966.1
Danio rerio (zebrafish)	bcmo1l	NP_956902.1
Arabidopsis thaliana (thale cress)	CCD8	NP_195007.2
Oryza sativa (rice)	Os01g0746400	NP_001044229.2
Magnaporthe oryzae (rice blast fungus)	MGG_08016	XP_003714968.1
Bos taurus (cattle)	BCMO1	NP_001019730.1
Mus musculus (house mouse)	Bcmo1	NP_067461.2
Rattus norvegicus (Norway rat)	Bcmo1	NP_446100.2
Caenorhabditis elegans (roundworm)	bcmo-1	NP_496729.2
Caenorhabditis elegans (roundworm)	bcmo-2	NP_494694.2
Neurospora crassa	NCU11424	XP_001727958.1
Pan troglodytes (chimpanzee)	BCMO1	XP_523435.3
Xenopus tropicalis (tropical clawed frog)	bcmo1	XP_002938186.1

Gene Ontology
Bibliography

Related articles in PubMed

Retinal accumulation of zeaxanthin, lutein, and ?-carotene in mice deficient in carotenoid cleavage enzymes.
Li B, Vachali PP, Shen Z, Gorusupudi A, Nelson K, Besch BM, Bartschi A, Longo S, Mattinson T, Shihab S, Polyakov NE, Suntsova LP, Dushkin AV, Bernstein PS
Experimental eye research159123-131(2017 06)

Inhibitory effects of fenretinide metabolites N-[4-methoxyphenyl]retinamide (MPR) and 4-oxo-N-(4-hydroxyphenyl)retinamide (3-keto-HPR) on fenretinide molecular targets ?-carotene oxygenase 1, stearoyl-CoA desaturase 1 and dihydroceramide ?4-desaturase 1.
Poliakov E, Samuel W, Duncan T, Gutierrez DB, Mata NL, Redmond TM
PloS one12(4)e0176487(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This is the first demonstration that Bco1 disruption lowers murine circulating testosterone levels and thereby reduces prostatic androgen receptor signaling and prostatic cellular proliferation, further supporting the role of this protein in processes more diverse than carotenoid cleavage.
Title: Mice lacking ?-carotene-15,15'-dioxygenase exhibit reduced serum testosterone, prostatic androgen receptor signaling, and prostatic cellular proliferation.

tissue- and sex-specific effects of beta-carotene 15,15' oxygenase (BCO1) on retinoid and lipid metabolism
Title: Tissue- and sex-specific effects of ?-carotene 15,15' oxygenase (BCO1) on retinoid and lipid metabolism in adult and developing mice.

BCO1 modulates heart metabolism and function, possibly by altering levels of cofactors required for the actions of nuclear hormone receptors
Title: Cardiac dysfunction in ?-carotene-15,15'-dioxygenase-deficient mice is associated with altered retinoid and lipid metabolism.

Dietary wolfberry elevated the xanthophyll concentrations and enhanced expression of BCO2 and heat shock protein 60, activated AMPKalpha2, potentiated mitophagy and mitochondrial biogenesis and enhanced lipid oxidation and secretion in the liver of mice.
Title: Wolfberries potentiate mitophagy and enhance mitochondrial biogenesis leading to prevention of hepatic steatosis in obese mice: the role of AMP-activated protein kinase ?2 subunit.

a stepwise cleavage by BCO2 and BCO1 with APO10ol as an intermediate could provide a mechanism to tailor asymmetric carotenoids such as beta-cryptoxanthin for vitamin A production.
Title: Two carotenoid oxygenases contribute to mammalian provitamin A metabolism.

The following Bco1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Bco1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu19487	NM_001163028.1 Latest version!	Mus musculus beta-carotene oxygenase 1 (Bco1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu19519	NM_021486.3 Latest version!	Mus musculus beta-carotene oxygenase 1 (Bco1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu19487	XM_011248481.2 Latest version!	Mus musculus beta-carotene oxygenase 1 (Bco1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Bco1 ( NM_001163028.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001156500.1 Mus musculus beta-carotene oxygenase 1 (Bco1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu19487
Clone ID Related Accession (Same CDS sequence)	XM_011248481.2 , NM_001163028.1
Accession Version	NM_001163028.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1581bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	beta,beta-carotene 15,15'-dioxygenase isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC108436.5 and AC121116.7. Transcript Variant: This variant (2) includes an alternate exon in the 3' coding region resulting in a frameshift, compared to variant 1. It encodes isoform 2 which has a shorter and distinct C-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC145036.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849384, SAMN01164140 [ECO:0000348] ##Evidence-Data-END##

ATGGAGATAA TATTTGGCCA GAATAAGAAA GAACAGCTGG AGCCAGTTCA GGCCAAAGTG 
ACAGGCAGCA TTCCAGCATG GCTGCAGGGG ACCCTGCTCC GAAACGGGCC CGGGATGCAC 
ACAGTGGGAG AGAGCAAGTA CAACCATTGG TTTGATGGCC TGGCCCTTCT CCACAGTTTC 
TCCATCAGAG ATGGGGAGGT CTTCTACAGG AGCAAATACC TGCAGAGTGA CACCTACATC 
GCCAACATTG AGGCCAACAG AATCGTGGTG TCTGAGTTCG GAACCATGGC CTACCCGGAC 
CCCTGCAAAA ACATCTTTTC CAAAGCTTTC TCCTACTTGT CTCACACCAT CCCCGACTTC 
ACAGACAACT GTCTGATCAA CATCATGAAA TGTGGAGAAG ACTTCTATGC AACCACGGAG 
ACCAACTACA TCAGGAAAAT CGACCCCCAG ACCCTAGAGA CCTTGGAGAA GGTTGATTAC 
CGGAAGTATG TGGCGGTAAA CCTGGCTACC TCGCACCCTC ATTATGACGA GGCTGGGAAT 
GTCCTTAACA TGGGCACATC CGTCGTGGAC AAAGGGAGGA CAAAATACGT GATATTTAAG 
ATCCCTGCCA CAGTGCCAGA CAGCAAGAAG AAAGGGAAGA GTCCCGTGAA GCACGCGGAA 
GTTTTCTGCT CCATTTCCTC CCGCTCGTTG CTCTCTCCCA GCTACTACCA CAGCTTTGGT 
GTCACGGAGA ACTATGTGGT GTTTCTGGAG CAGCCTTTTA AGTTGGATAT CCTCAAGATG 
GCCACCGCAT ACATGAGGGG AGTGAGCTGG GCTTCCTGTA TGTCATTCGA CAGGGAGGAC 
AAGACATACA TTCATATCAT CGACCAGAGG ACCAGGAAGC CTGTGCCTAC CAAGTTCTAC 
ACAGATCCCA TGGTGGTCTT CCATCATGTC AATGCCTACG AGGAGGACGG CTGTGTGCTG 
TTTGATGTGA TCGCCTATGA GGACAGCAGC CTCTATCAGC TCTTCTACCT GGCCAACCTG 
AACAAGGACT TCGAGGAGAA GTCCAGGCTG ACCTCAGTGC CTACCCTCAG GAGGTTTGCT 
GTGCCCCTCC ATGTGGACAA GGATGCAGAA GTGGGCTCAA ATTTAGTCAA GGTGTCATCT 
ACAACTGCAA CAGCCCTGAA GGAGAAAGAC GGCCATGTCT ATTGCCAGCC CGAGGTCCTC 
TACGAAGGCC TAGAGCTCCC TCGGATAAAT TATGCTTACA ACGGGAAGCC ATATCGCTAC 
ATCTTTGCAG CTGAAGTACA GTGGAGTCCA GTCCCAACCA AGATACTGAA ATATGACATT 
CTCACAAAGT CCTCCTTAAA GTGGTCTGAG GAGAGCTGCT GGCCAGCAGA GCCTCTGTTT 
GTTCCCACGC CAGGTGCGAA GGATGAAGAT GATGTCCCTT GCAGGCCAAG CAGAGACTGT 
GGAGTCCAAG GGCCCCATCG TAAATCACAT TGTCAGCTGA GCATAGCATG TGTGCCTGAG 
ATCTCAGCAT TTGGGAGGCA GACAGAAGAC AATCAGGGGT CTACAGTCAT TCTCAGATAT 
ACAGAGAATT TGAACTACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001156500.1
CDS	167..1747
Translation	MEIIFGQNKKEQLEPVQAKVTGSIPAWLQGTLLRNGPGMHTVGESKYNHWFDGLALLHSFSIRDGEVFYRSKYLQSDTYIANIEANRIVVSEFGTMAYPDPCKNIFSKAFSYLSHTIPDFTDNCLINIMKCGEDFYATTETNYIRKIDPQTLETLEKVDYRKYVAVNLATSHPHYDEAGNVLNMGTSVVDKGRTKYVIFKIPATVPDSKKKGKSPVKHAEVFCSISSRSLLSPSYYHSFGVTENYVVFLEQPFKLDILKMATAYMRGVSWASCMSFDREDKTYIHIIDQRTRKPVPTKFYTDPMVVFHHVNAYEEDGCVLFDVIAYEDSSLYQLFYLANLNKDFEEKSRLTSVPTLRRFAVPLHVDKDAEVGSNLVKVSSTTATALKEKDGHVYCQPEVLYEGLELPRINYAYNGKPYRYIFAAEVQWSPVPTKILKYDILTKSSLKWSEESCWPAEPLFVPTPGAKDEDDVPCRPSRDCGVQGPHRKSHCQLSIACVPEISAFGRQTEDNQGSTVILRYTENLNY

Target ORF information:

RefSeq Version	NM_001163028.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus beta-carotene oxygenase 1 (Bco1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001163028.1

ORF Insert Sequence:

ATGGAGATAA TATTTGGCCA GAATAAGAAA GAACAGCTGG AGCCAGTTCA GGCCAAAGTG 
ACAGGCAGCA TTCCAGCATG GCTGCAGGGG ACCCTGCTCC GAAACGGGCC CGGGATGCAC 
ACAGTGGGAG AGAGCAAGTA CAACCATTGG TTTGATGGCC TGGCCCTTCT CCACAGTTTC 
TCCATCAGAG ATGGGGAGGT CTTCTACAGG AGCAAATACC TGCAGAGTGA CACCTACATC 
GCCAACATTG AGGCCAACAG AATCGTGGTG TCTGAGTTCG GAACCATGGC CTACCCGGAC 
CCCTGCAAAA ACATCTTTTC CAAAGCTTTC TCCTACTTGT CTCACACCAT CCCCGACTTC 
ACAGACAACT GTCTGATCAA CATCATGAAA TGTGGAGAAG ACTTCTATGC AACCACGGAG 
ACCAACTACA TCAGGAAAAT CGACCCCCAG ACCCTAGAGA CCTTGGAGAA GGTTGATTAC 
CGGAAGTATG TGGCGGTAAA CCTGGCTACC TCGCACCCTC ATTATGACGA GGCTGGGAAT 
GTCCTTAACA TGGGCACATC CGTCGTGGAC AAAGGGAGGA CAAAATACGT GATATTTAAG 
ATCCCTGCCA CAGTGCCAGA CAGCAAGAAG AAAGGGAAGA GTCCCGTGAA GCACGCGGAA 
GTTTTCTGCT CCATTTCCTC CCGCTCGTTG CTCTCTCCCA GCTACTACCA CAGCTTTGGT 
GTCACGGAGA ACTATGTGGT GTTTCTGGAG CAGCCTTTTA AGTTGGATAT CCTCAAGATG 
GCCACCGCAT ACATGAGGGG AGTGAGCTGG GCTTCCTGTA TGTCATTCGA CAGGGAGGAC 
AAGACATACA TTCATATCAT CGACCAGAGG ACCAGGAAGC CTGTGCCTAC CAAGTTCTAC 
ACAGATCCCA TGGTGGTCTT CCATCATGTC AATGCCTACG AGGAGGACGG CTGTGTGCTG 
TTTGATGTGA TCGCCTATGA GGACAGCAGC CTCTATCAGC TCTTCTACCT GGCCAACCTG 
AACAAGGACT TCGAGGAGAA GTCCAGGCTG ACCTCAGTGC CTACCCTCAG GAGGTTTGCT 
GTGCCCCTCC ATGTGGACAA GGATGCAGAA GTGGGCTCAA ATTTAGTCAA GGTGTCATCT 
ACAACTGCAA CAGCCCTGAA GGAGAAAGAC GGCCATGTCT ATTGCCAGCC CGAGGTCCTC 
TACGAAGGCC TAGAGCTCCC TCGGATAAAT TATGCTTACA ACGGGAAGCC ATATCGCTAC 
ATCTTTGCAG CTGAAGTACA GTGGAGTCCA GTCCCAACCA AGATACTGAA ATATGACATT 
CTCACAAAGT CCTCCTTAAA GTGGTCTGAG GAGAGCTGCT GGCCAGCAGA GCCTCTGTTT 
GTTCCCACGC CAGGTGCGAA GGATGAAGAT GATGTCCCTT GCAGGCCAAG CAGAGACTGT 
GGAGTCCAAG GGCCCCATCG TAAATCACAT TGTCAGCTGA GCATAGCATG TGTGCCTGAG 
ATCTCAGCAT TTGGGAGGCA GACAGAAGAC AATCAGGGGT CTACAGTCAT TCTCAGATAT 
ACAGAGAATT TGAACTACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bco1 ( NM_021486.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_067461.2 Mus musculus beta-carotene oxygenase 1 (Bco1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu19519
Clone ID Related Accession (Same CDS sequence)	NM_021486.3
Accession Version	NM_021486.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1701bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Mus musculus(house mouse)
Product	beta,beta-carotene 15,15'-dioxygenase isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC108436.5, AK146472.1, AK054171.1 and AC121116.7. On Jul 9, 2009 this sequence version replaced NM_021486.2. Transcript Variant: This variant (1) represents the shorter transcript but encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK146472.1, AF271298.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849384 [ECO:0000348] ##Evidence-Data-END##

ATGGAGATAA TATTTGGCCA GAATAAGAAA GAACAGCTGG AGCCAGTTCA GGCCAAAGTG 
ACAGGCAGCA TTCCAGCATG GCTGCAGGGG ACCCTGCTCC GAAACGGGCC CGGGATGCAC 
ACAGTGGGAG AGAGCAAGTA CAACCATTGG TTTGATGGCC TGGCCCTTCT CCACAGTTTC 
TCCATCAGAG ATGGGGAGGT CTTCTACAGG AGCAAATACC TGCAGAGTGA CACCTACATC 
GCCAACATTG AGGCCAACAG AATCGTGGTG TCTGAGTTCG GAACCATGGC CTACCCGGAC 
CCCTGCAAAA ACATCTTTTC CAAAGCTTTC TCCTACTTGT CTCACACCAT CCCCGACTTC 
ACAGACAACT GTCTGATCAA CATCATGAAA TGTGGAGAAG ACTTCTATGC AACCACGGAG 
ACCAACTACA TCAGGAAAAT CGACCCCCAG ACCCTAGAGA CCTTGGAGAA GGTTGATTAC 
CGGAAGTATG TGGCGGTAAA CCTGGCTACC TCGCACCCTC ATTATGACGA GGCTGGGAAT 
GTCCTTAACA TGGGCACATC CGTCGTGGAC AAAGGGAGGA CAAAATACGT GATATTTAAG 
ATCCCTGCCA CAGTGCCAGA CAGCAAGAAG AAAGGGAAGA GTCCCGTGAA GCACGCGGAA 
GTTTTCTGCT CCATTTCCTC CCGCTCGTTG CTCTCTCCCA GCTACTACCA CAGCTTTGGT 
GTCACGGAGA ACTATGTGGT GTTTCTGGAG CAGCCTTTTA AGTTGGATAT CCTCAAGATG 
GCCACCGCAT ACATGAGGGG AGTGAGCTGG GCTTCCTGTA TGTCATTCGA CAGGGAGGAC 
AAGACATACA TTCATATCAT CGACCAGAGG ACCAGGAAGC CTGTGCCTAC CAAGTTCTAC 
ACAGATCCCA TGGTGGTCTT CCATCATGTC AATGCCTACG AGGAGGACGG CTGTGTGCTG 
TTTGATGTGA TCGCCTATGA GGACAGCAGC CTCTATCAGC TCTTCTACCT GGCCAACCTG 
AACAAGGACT TCGAGGAGAA GTCCAGGCTG ACCTCAGTGC CTACCCTCAG GAGGTTTGCT 
GTGCCCCTCC ATGTGGACAA GGATGCAGAA GTGGGCTCAA ATTTAGTCAA GGTGTCATCT 
ACAACTGCAA CAGCCCTGAA GGAGAAAGAC GGCCATGTCT ATTGCCAGCC CGAGGTCCTC 
TACGAAGGCC TAGAGCTCCC TCGGATAAAT TATGCTTACA ACGGGAAGCC ATATCGCTAC 
ATCTTTGCAG CTGAAGTACA GTGGAGTCCA GTCCCAACCA AGATACTGAA ATATGACATT 
CTCACAAAGT CCTCCTTAAA GTGGTCTGAG GAGAGCTGCT GGCCAGCAGA GCCTCTGTTT 
GTTCCCACGC CAGGTGCGAA GGATGAAGAT GATGGAGTCA TTTTATCAGC CATCGTCTCT 
ACGGATCCCC AAAAGCTGCC TTTTTTACTC ATTCTGGATG CCAAAAGTTT TACGGAACTG 
GCTCGCGCCT CTGTTGATGC GGACATGCAC CTGGACCTTC ATGGTTTATT TATCCCAGAT 
GCAGACTGGA ATGCAGTGAA GCAGACTCCA GCTGAAACGC AAGAGGTTGA AAACTCAGAT 
CATCCCACAG ATCCGACAGC ACCAGAACTG AGCCACAGTG AAAATGACTT CACAGCGGGT 
CATGGTGGCT CAAGTCTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067461.2
CDS	167..1867
Translation	MEIIFGQNKKEQLEPVQAKVTGSIPAWLQGTLLRNGPGMHTVGESKYNHWFDGLALLHSFSIRDGEVFYRSKYLQSDTYIANIEANRIVVSEFGTMAYPDPCKNIFSKAFSYLSHTIPDFTDNCLINIMKCGEDFYATTETNYIRKIDPQTLETLEKVDYRKYVAVNLATSHPHYDEAGNVLNMGTSVVDKGRTKYVIFKIPATVPDSKKKGKSPVKHAEVFCSISSRSLLSPSYYHSFGVTENYVVFLEQPFKLDILKMATAYMRGVSWASCMSFDREDKTYIHIIDQRTRKPVPTKFYTDPMVVFHHVNAYEEDGCVLFDVIAYEDSSLYQLFYLANLNKDFEEKSRLTSVPTLRRFAVPLHVDKDAEVGSNLVKVSSTTATALKEKDGHVYCQPEVLYEGLELPRINYAYNGKPYRYIFAAEVQWSPVPTKILKYDILTKSSLKWSEESCWPAEPLFVPTPGAKDEDDGVILSAIVSTDPQKLPFLLILDAKSFTELARASVDADMHLDLHGLFIPDADWNAVKQTPAETQEVENSDHPTDPTAPELSHSENDFTAGHGGSSL

Target ORF information:

RefSeq Version	NM_021486.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus beta-carotene oxygenase 1 (Bco1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021486.3

ORF Insert Sequence:

ATGGAGATAA TATTTGGCCA GAATAAGAAA GAACAGCTGG AGCCAGTTCA GGCCAAAGTG 
ACAGGCAGCA TTCCAGCATG GCTGCAGGGG ACCCTGCTCC GAAACGGGCC CGGGATGCAC 
ACAGTGGGAG AGAGCAAGTA CAACCATTGG TTTGATGGCC TGGCCCTTCT CCACAGTTTC 
TCCATCAGAG ATGGGGAGGT CTTCTACAGG AGCAAATACC TGCAGAGTGA CACCTACATC 
GCCAACATTG AGGCCAACAG AATCGTGGTG TCTGAGTTCG GAACCATGGC CTACCCGGAC 
CCCTGCAAAA ACATCTTTTC CAAAGCTTTC TCCTACTTGT CTCACACCAT CCCCGACTTC 
ACAGACAACT GTCTGATCAA CATCATGAAA TGTGGAGAAG ACTTCTATGC AACCACGGAG 
ACCAACTACA TCAGGAAAAT CGACCCCCAG ACCCTAGAGA CCTTGGAGAA GGTTGATTAC 
CGGAAGTATG TGGCGGTAAA CCTGGCTACC TCGCACCCTC ATTATGACGA GGCTGGGAAT 
GTCCTTAACA TGGGCACATC CGTCGTGGAC AAAGGGAGGA CAAAATACGT GATATTTAAG 
ATCCCTGCCA CAGTGCCAGA CAGCAAGAAG AAAGGGAAGA GTCCCGTGAA GCACGCGGAA 
GTTTTCTGCT CCATTTCCTC CCGCTCGTTG CTCTCTCCCA GCTACTACCA CAGCTTTGGT 
GTCACGGAGA ACTATGTGGT GTTTCTGGAG CAGCCTTTTA AGTTGGATAT CCTCAAGATG 
GCCACCGCAT ACATGAGGGG AGTGAGCTGG GCTTCCTGTA TGTCATTCGA CAGGGAGGAC 
AAGACATACA TTCATATCAT CGACCAGAGG ACCAGGAAGC CTGTGCCTAC CAAGTTCTAC 
ACAGATCCCA TGGTGGTCTT CCATCATGTC AATGCCTACG AGGAGGACGG CTGTGTGCTG 
TTTGATGTGA TCGCCTATGA GGACAGCAGC CTCTATCAGC TCTTCTACCT GGCCAACCTG 
AACAAGGACT TCGAGGAGAA GTCCAGGCTG ACCTCAGTGC CTACCCTCAG GAGGTTTGCT 
GTGCCCCTCC ATGTGGACAA GGATGCAGAA GTGGGCTCAA ATTTAGTCAA GGTGTCATCT 
ACAACTGCAA CAGCCCTGAA GGAGAAAGAC GGCCATGTCT ATTGCCAGCC CGAGGTCCTC 
TACGAAGGCC TAGAGCTCCC TCGGATAAAT TATGCTTACA ACGGGAAGCC ATATCGCTAC 
ATCTTTGCAG CTGAAGTACA GTGGAGTCCA GTCCCAACCA AGATACTGAA ATATGACATT 
CTCACAAAGT CCTCCTTAAA GTGGTCTGAG GAGAGCTGCT GGCCAGCAGA GCCTCTGTTT 
GTTCCCACGC CAGGTGCGAA GGATGAAGAT GATGGAGTCA TTTTATCAGC CATCGTCTCT 
ACGGATCCCC AAAAGCTGCC TTTTTTACTC ATTCTGGATG CCAAAAGTTT TACGGAACTG 
GCTCGCGCCT CTGTTGATGC GGACATGCAC CTGGACCTTC ATGGTTTATT TATCCCAGAT 
GCAGACTGGA ATGCAGTGAA GCAGACTCCA GCTGAAACGC AAGAGGTTGA AAACTCAGAT 
CATCCCACAG ATCCGACAGC ACCAGAACTG AGCCACAGTG AAAATGACTT CACAGCGGGT 
CATGGTGGCT CAAGTCTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bco1 ( XM_011248481.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246783.1 Mus musculus beta-carotene oxygenase 1 (Bco1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu19487
Clone ID Related Accession (Same CDS sequence)	XM_011248481.2 , NM_001163028.1
Accession Version	XM_011248481.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1581bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	beta,beta-carotene 15,15'-dioxygenase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011248481.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGATAA TATTTGGCCA GAATAAGAAA GAACAGCTGG AGCCAGTTCA GGCCAAAGTG 
ACAGGCAGCA TTCCAGCATG GCTGCAGGGG ACCCTGCTCC GAAACGGGCC CGGGATGCAC 
ACAGTGGGAG AGAGCAAGTA CAACCATTGG TTTGATGGCC TGGCCCTTCT CCACAGTTTC 
TCCATCAGAG ATGGGGAGGT CTTCTACAGG AGCAAATACC TGCAGAGTGA CACCTACATC 
GCCAACATTG AGGCCAACAG AATCGTGGTG TCTGAGTTCG GAACCATGGC CTACCCGGAC 
CCCTGCAAAA ACATCTTTTC CAAAGCTTTC TCCTACTTGT CTCACACCAT CCCCGACTTC 
ACAGACAACT GTCTGATCAA CATCATGAAA TGTGGAGAAG ACTTCTATGC AACCACGGAG 
ACCAACTACA TCAGGAAAAT CGACCCCCAG ACCCTAGAGA CCTTGGAGAA GGTTGATTAC 
CGGAAGTATG TGGCGGTAAA CCTGGCTACC TCGCACCCTC ATTATGACGA GGCTGGGAAT 
GTCCTTAACA TGGGCACATC CGTCGTGGAC AAAGGGAGGA CAAAATACGT GATATTTAAG 
ATCCCTGCCA CAGTGCCAGA CAGCAAGAAG AAAGGGAAGA GTCCCGTGAA GCACGCGGAA 
GTTTTCTGCT CCATTTCCTC CCGCTCGTTG CTCTCTCCCA GCTACTACCA CAGCTTTGGT 
GTCACGGAGA ACTATGTGGT GTTTCTGGAG CAGCCTTTTA AGTTGGATAT CCTCAAGATG 
GCCACCGCAT ACATGAGGGG AGTGAGCTGG GCTTCCTGTA TGTCATTCGA CAGGGAGGAC 
AAGACATACA TTCATATCAT CGACCAGAGG ACCAGGAAGC CTGTGCCTAC CAAGTTCTAC 
ACAGATCCCA TGGTGGTCTT CCATCATGTC AATGCCTACG AGGAGGACGG CTGTGTGCTG 
TTTGATGTGA TCGCCTATGA GGACAGCAGC CTCTATCAGC TCTTCTACCT GGCCAACCTG 
AACAAGGACT TCGAGGAGAA GTCCAGGCTG ACCTCAGTGC CTACCCTCAG GAGGTTTGCT 
GTGCCCCTCC ATGTGGACAA GGATGCAGAA GTGGGCTCAA ATTTAGTCAA GGTGTCATCT 
ACAACTGCAA CAGCCCTGAA GGAGAAAGAC GGCCATGTCT ATTGCCAGCC CGAGGTCCTC 
TACGAAGGCC TAGAGCTCCC TCGGATAAAT TATGCTTACA ACGGGAAGCC ATATCGCTAC 
ATCTTTGCAG CTGAAGTACA GTGGAGTCCA GTCCCAACCA AGATACTGAA ATATGACATT 
CTCACAAAGT CCTCCTTAAA GTGGTCTGAG GAGAGCTGCT GGCCAGCAGA GCCTCTGTTT 
GTTCCCACGC CAGGTGCGAA GGATGAAGAT GATGTCCCTT GCAGGCCAAG CAGAGACTGT 
GGAGTCCAAG GGCCCCATCG TAAATCACAT TGTCAGCTGA GCATAGCATG TGTGCCTGAG 
ATCTCAGCAT TTGGGAGGCA GACAGAAGAC AATCAGGGGT CTACAGTCAT TCTCAGATAT 
ACAGAGAATT TGAACTACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246783.1
CDS	181..1761
Translation	MEIIFGQNKKEQLEPVQAKVTGSIPAWLQGTLLRNGPGMHTVGESKYNHWFDGLALLHSFSIRDGEVFYRSKYLQSDTYIANIEANRIVVSEFGTMAYPDPCKNIFSKAFSYLSHTIPDFTDNCLINIMKCGEDFYATTETNYIRKIDPQTLETLEKVDYRKYVAVNLATSHPHYDEAGNVLNMGTSVVDKGRTKYVIFKIPATVPDSKKKGKSPVKHAEVFCSISSRSLLSPSYYHSFGVTENYVVFLEQPFKLDILKMATAYMRGVSWASCMSFDREDKTYIHIIDQRTRKPVPTKFYTDPMVVFHHVNAYEEDGCVLFDVIAYEDSSLYQLFYLANLNKDFEEKSRLTSVPTLRRFAVPLHVDKDAEVGSNLVKVSSTTATALKEKDGHVYCQPEVLYEGLELPRINYAYNGKPYRYIFAAEVQWSPVPTKILKYDILTKSSLKWSEESCWPAEPLFVPTPGAKDEDDVPCRPSRDCGVQGPHRKSHCQLSIACVPEISAFGRQTEDNQGSTVILRYTENLNY

Target ORF information:

RefSeq Version	XM_011248481.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus beta-carotene oxygenase 1 (Bco1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248481.2

ORF Insert Sequence:

ATGGAGATAA TATTTGGCCA GAATAAGAAA GAACAGCTGG AGCCAGTTCA GGCCAAAGTG 
ACAGGCAGCA TTCCAGCATG GCTGCAGGGG ACCCTGCTCC GAAACGGGCC CGGGATGCAC 
ACAGTGGGAG AGAGCAAGTA CAACCATTGG TTTGATGGCC TGGCCCTTCT CCACAGTTTC 
TCCATCAGAG ATGGGGAGGT CTTCTACAGG AGCAAATACC TGCAGAGTGA CACCTACATC 
GCCAACATTG AGGCCAACAG AATCGTGGTG TCTGAGTTCG GAACCATGGC CTACCCGGAC 
CCCTGCAAAA ACATCTTTTC CAAAGCTTTC TCCTACTTGT CTCACACCAT CCCCGACTTC 
ACAGACAACT GTCTGATCAA CATCATGAAA TGTGGAGAAG ACTTCTATGC AACCACGGAG 
ACCAACTACA TCAGGAAAAT CGACCCCCAG ACCCTAGAGA CCTTGGAGAA GGTTGATTAC 
CGGAAGTATG TGGCGGTAAA CCTGGCTACC TCGCACCCTC ATTATGACGA GGCTGGGAAT 
GTCCTTAACA TGGGCACATC CGTCGTGGAC AAAGGGAGGA CAAAATACGT GATATTTAAG 
ATCCCTGCCA CAGTGCCAGA CAGCAAGAAG AAAGGGAAGA GTCCCGTGAA GCACGCGGAA 
GTTTTCTGCT CCATTTCCTC CCGCTCGTTG CTCTCTCCCA GCTACTACCA CAGCTTTGGT 
GTCACGGAGA ACTATGTGGT GTTTCTGGAG CAGCCTTTTA AGTTGGATAT CCTCAAGATG 
GCCACCGCAT ACATGAGGGG AGTGAGCTGG GCTTCCTGTA TGTCATTCGA CAGGGAGGAC 
AAGACATACA TTCATATCAT CGACCAGAGG ACCAGGAAGC CTGTGCCTAC CAAGTTCTAC 
ACAGATCCCA TGGTGGTCTT CCATCATGTC AATGCCTACG AGGAGGACGG CTGTGTGCTG 
TTTGATGTGA TCGCCTATGA GGACAGCAGC CTCTATCAGC TCTTCTACCT GGCCAACCTG 
AACAAGGACT TCGAGGAGAA GTCCAGGCTG ACCTCAGTGC CTACCCTCAG GAGGTTTGCT 
GTGCCCCTCC ATGTGGACAA GGATGCAGAA GTGGGCTCAA ATTTAGTCAA GGTGTCATCT 
ACAACTGCAA CAGCCCTGAA GGAGAAAGAC GGCCATGTCT ATTGCCAGCC CGAGGTCCTC 
TACGAAGGCC TAGAGCTCCC TCGGATAAAT TATGCTTACA ACGGGAAGCC ATATCGCTAC 
ATCTTTGCAG CTGAAGTACA GTGGAGTCCA GTCCCAACCA AGATACTGAA ATATGACATT 
CTCACAAAGT CCTCCTTAAA GTGGTCTGAG GAGAGCTGCT GGCCAGCAGA GCCTCTGTTT 
GTTCCCACGC CAGGTGCGAA GGATGAAGAT GATGTCCCTT GCAGGCCAAG CAGAGACTGT 
GGAGTCCAAG GGCCCCATCG TAAATCACAT TGTCAGCTGA GCATAGCATG TGTGCCTGAG 
ATCTCAGCAT TTGGGAGGCA GACAGAAGAC AATCAGGGGT CTACAGTCAT TCTCAGATAT 
ACAGAGAATT TGAACTACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Retinal accumulation of zeaxanthin, lutein, and ?-carotene in mice deficient in carotenoid cleavage enzymes.
Experimental eye research159123-131(2017 06)
Li B,Vachali PP,Shen Z,Gorusupudi A,Nelson K,Besch BM,Bartschi A,Longo S,Mattinson T,Shihab S,Polyakov NE,Suntsova LP,Dushkin AV,Bernstein PS

Inhibitory effects of fenretinide metabolites N-[4-methoxyphenyl]retinamide (MPR) and 4-oxo-N-(4-hydroxyphenyl)retinamide (3-keto-HPR) on fenretinide molecular targets ?-carotene oxygenase 1, stearoyl-CoA desaturase 1 and dihydroceramide ?4-desaturase 1.
PloS one12(4)e0176487(2017)
Poliakov E,Samuel W,Duncan T,Gutierrez DB,Mata NL,Redmond TM

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Bco1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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