Defb42 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Defb42
Entrez Gene ID	619548
Full Name	defensin beta 42
Synonyms	BD-42,Defb44,mBD-42
General protein information	Preferred Names defensin beta 42 Names beta-defensin 42 beta-defensin 136 beta-defensin 44
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 14 Map Location: 14\|14 D1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001034910.3	NP_001030082.1	beta-defensin 42 precursor
XM_030247907.1	XP_030103767.1	beta-defensin 42 isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Defb42	NP_001030082.1
Rattus norvegicus (Norway rat)	Defb44	NP_001032618.1

Gene Ontology
Bibliography

Related articles in PubMed

Manual annotation and analysis of the defensin gene cluster in the C57BL/6J mouse reference genome.
Amid C, Rehaume LM, Brown KL, Gilbert JG, Dougan G, Hancock RE, Harrow JL
BMC genomics10606(2009 Dec)

Functional dynamics of H3K9 methylation during meiotic prophase progression.
Tachibana M, Nozaki M, Takeda N, Shinkai Y
The EMBO journal26(14)3346-59(2007 Jul)

Cross-species analysis of the mammalian beta-defensin gene family: presence of syntenic gene clusters and preferential expression in the male reproductive tract.
Patil AA, Cai Y, Sang Y, Blecha F, Zhang G
Physiological genomics23(1)5-17(2005 Sep)

Discovery and characterization of new epididymis-specific beta-defensins in mice.
Jalkanen J, Huhtaniemi I, Poutanen M
Biochimica et biophysica acta1730(1)22-30(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Defb41 and Defb42 are suggested to have a role in the antimicrobial protection of sperm and urogenital tract epithelia. [Defb42]
Title: Discovery and characterization of new epididymis-specific beta-defensins in mice.

The following Defb42 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Defb42 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu12795	NM_001034910.3 Latest version!	Mus musculus defensin beta 42 (Defb42), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu12795	XM_030247907.1 Latest version!	Mus musculus defensin beta 42 (Defb42), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Defb42 ( NM_001034910.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001030082.1 Mus musculus defensin beta 42 (Defb42), mRNA.

Price & Availability↑

CloneID	OMu12795
Clone ID Related Accession (Same CDS sequence)	NM_001034910.3 , XM_030247907.1
Accession Version	NM_001034910.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 228bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Mus musculus(house mouse)
Product	beta-defensin 42 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK079042.1 and AI463041.1. On Apr 29, 2010 this sequence version replaced NM_001034910.2. ##Evidence-Data-START## Transcript exon combination :: DQ012046.1, BB625617.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849376 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACCTGC GTCTTTCATG CTTACTGTTC ATCCTGGTGA CATCATTGCC TGCAGGTCGC 
TGTTCGATCG GGAATAAGGG CATATCCTTT GAGACGTGCA CCGCAATCGA AGGCTTGTGT 
TTCTTTGGTT GTAAGTTAGG GTGGGTATGG ATTGCCTACT GTAACAACAT AATGTCCTGT 
TGTAGAAAGG ATACCGATTT TGTACTTCCT CAAACCAAAG GTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001030082.1
CDS	181..408
Translation	MNLRLSCLLFILVTSLPAGRCSIGNKGISFETCTAIEGLCFFGCKLGWVWIAYCNNIMSCCRKDTDFVLPQTKGI

Target ORF information:

RefSeq Version	NM_001034910.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus defensin beta 42 (Defb42), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001034910.3

ORF Insert Sequence:

ATGAACCTGC GTCTTTCATG CTTACTGTTC ATCCTGGTGA CATCATTGCC TGCAGGTCGC 
TGTTCGATCG GGAATAAGGG CATATCCTTT GAGACGTGCA CCGCAATCGA AGGCTTGTGT 
TTCTTTGGTT GTAAGTTAGG GTGGGTATGG ATTGCCTACT GTAACAACAT AATGTCCTGT 
TGTAGAAAGG ATACCGATTT TGTACTTCCT CAAACCAAAG GTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Defb42 ( XM_030247907.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030103767.1 Mus musculus defensin beta 42 (Defb42), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu12795
Clone ID Related Accession (Same CDS sequence)	NM_001034910.3 , XM_030247907.1
Accession Version	XM_030247907.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 228bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	beta-defensin 42 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCTGC GTCTTTCATG CTTACTGTTC ATCCTGGTGA CATCATTGCC TGCAGGTCGC 
TGTTCGATCG GGAATAAGGG CATATCCTTT GAGACGTGCA CCGCAATCGA AGGCTTGTGT 
TTCTTTGGTT GTAAGTTAGG GTGGGTATGG ATTGCCTACT GTAACAACAT AATGTCCTGT 
TGTAGAAAGG ATACCGATTT TGTACTTCCT CAAACCAAAG GTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030103767.1
CDS	219..446
Translation	MNLRLSCLLFILVTSLPAGRCSIGNKGISFETCTAIEGLCFFGCKLGWVWIAYCNNIMSCCRKDTDFVLPQTKGI

Target ORF information:

RefSeq Version	XM_030247907.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus defensin beta 42 (Defb42), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030247907.1

ORF Insert Sequence:

ATGAACCTGC GTCTTTCATG CTTACTGTTC ATCCTGGTGA CATCATTGCC TGCAGGTCGC 
TGTTCGATCG GGAATAAGGG CATATCCTTT GAGACGTGCA CCGCAATCGA AGGCTTGTGT 
TTCTTTGGTT GTAAGTTAGG GTGGGTATGG ATTGCCTACT GTAACAACAT AATGTCCTGT 
TGTAGAAAGG ATACCGATTT TGTACTTCCT CAAACCAAAG GTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Manual annotation and analysis of the defensin gene cluster in the C57BL/6J mouse reference genome.
BMC genomics10606(2009 Dec)
Amid C,Rehaume LM,Brown KL,Gilbert JG,Dougan G,Hancock RE,Harrow JL

Functional dynamics of H3K9 methylation during meiotic prophase progression.
The EMBO journal26(14)3346-59(2007 Jul)
Tachibana M,Nozaki M,Takeda N,Shinkai Y

Cross-species analysis of the mammalian beta-defensin gene family: presence of syntenic gene clusters and preferential expression in the male reproductive tract.
Physiological genomics23(1)5-17(2005 Sep)
Patil AA,Cai Y,Sang Y,Blecha F,Zhang G

Discovery and characterization of new epididymis-specific beta-defensins in mice.
Biochimica et biophysica acta1730(1)22-30(2005 Jul)
Jalkanen J,Huhtaniemi I,Poutanen M