Trim54 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Trim54
Entrez Gene ID	58522
Full Name	tripartite motif-containing 54
Synonyms	4930486E09Rik,4930566I02Rik,MURF,MURF-3,MuRF3,Rnf30
General protein information	Preferred Names tripartite motif-containing 54 Names tripartite motif-containing protein 54 muscle-specific RING finger protein 3 muscle-specific RING-finger protein ring finger protein 30
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 B1

mRNA and Protein(s)

mRNA	Protein	Name
NM_021447.2	NP_067422.1	tripartite motif-containing protein 54

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Trim54	NP_001013235.1
Bos taurus (cattle)	TRIM54	NP_001017951.1
Pan troglodytes (chimpanzee)	TRIM54	XP_001155913.1
Canis lupus familiaris (dog)	TRIM54	XP_005630313.1
Mus musculus (house mouse)	Trim54	NP_067422.1
Danio rerio (zebrafish)	trim54	NP_001038490.2
Homo sapiens (human)	TRIM54	NP_912730.2
	TRIM54	XP_001092313.1

Gene Ontology
Bibliography

Related articles in PubMed

Muscle ring finger-3 protects against diabetic cardiomyopathy induced by a high fat diet.
Quintana MT, He J, Sullivan J, Grevengoed T, Schisler J, Han Y, Hill JA, Yates CC, Stansfield WE, Mapanga RF, Essop MF, Muehlbauer MJ, Newgard CB, Bain JR, Willis MS
BMC endocrine disorders1536(2015 Jul)

Developmental regulation of MURF ubiquitin ligases and autophagy proteins nbr1, p62/SQSTM1 and LC3 during cardiac myofibril assembly and turnover.
Perera S, Holt MR, Mankoo BS, Gautel M
Developmental biology351(1)46-61(2011 Mar)

Muscle RING-finger protein-1 (MuRF1) as a connector of muscle energy metabolism and protein synthesis.
Koyama S, Hata S, Witt CC, Ono Y, Lerche S, Ojima K, Chiba T, Doi N, Kitamura F, Tanaka K, Abe K, Witt SH, Rybin V, Gasch A, Franz T, Labeit S, Sorimachi H
Journal of molecular biology376(5)1224-36(2008 Mar)

Myosin accumulation and striated muscle myopathy result from the loss of muscle RING finger 1 and 3.
Fielitz J, Kim MS, Shelton JM, Latif S, Spencer JA, Glass DJ, Richardson JA, Bassel-Duby R, Olson EN
The Journal of clinical investigation117(9)2486-95(2007 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

findings suggest that MuRF3 helps stabilize cardiac PPARalpha and PPARgamma1 in vivo to support resistance to the development of diabetic cardiomyopathy. MuRF3 also plays an unexpected role in regulating fat storage despite being found only in striated muscle.
Title: Muscle ring finger-3 protects against diabetic cardiomyopathy induced by a high fat diet.

Knockdown of both MURF2 and MURF3 severely disrupts the formation of ordered Z- and M-bands, likely by perturbed tubulin dynamics
Title: Developmental regulation of MURF ubiquitin ligases and autophagy proteins nbr1, p62/SQSTM1 and LC3 during cardiac myofibril assembly and turnover.

muscle RING finger 1 and muscle RING finger 3 as key E3 ubiquitin ligases for the ubiquitin proteasome system-dependent turnover of sarcomeric proteins and reveal a potential basis for myosin storage myopathies
Title: Myosin accumulation and striated muscle myopathy result from the loss of muscle RING finger 1 and 3.

MuRF3 has an essential role in maintaining cardiac integrity and function after acute myocardial infarction and turnover of FHL2 and gamma-filamin contributes to this cardioprotective function of MuRF3
Title: Loss of muscle-specific RING-finger 3 predisposes the heart to cardiac rupture after myocardial infarction.

The following Trim54 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Trim54 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu12357	NM_021447.2 Latest version!	Mus musculus tripartite motif-containing 54 (Trim54), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Trim54 ( NM_021447.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_067422.1 Mus musculus tripartite motif-containing 54 (Trim54), mRNA.

Price & Availability↑

CloneID	OMu12357
Clone ID Related Accession (Same CDS sequence)	NM_021447.2
Accession Version	NM_021447.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1101bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Mus musculus(house mouse)
Product	tripartite motif-containing protein 54
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY346743.1 and AF294790.1. On Dec 1, 2007 this sequence version replaced NM_021447.1. ##Evidence-Data-START## Transcript exon combination :: AF294790.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849376 [ECO:0000348] ##Evidence-Data-END##

ATGAACTTCA CGGTGGGTTT CAAGCCGCTG CTAGGGGATG CGCACAACAT GGACAACTTG 
GAGAAGCAGC TCATTTGCCC CATCTGCCTG GAGATGTTCT CCAAGCCCGT GGTGATCTTG 
CCCTGCCAAC ACAACCTGTG CCGCAAGTGT GCCAACGACG TCTTCCAGGC CTCTAATCCT 
CTGTGGCAAT CCCGGGGCTC CACAACGGTG TCTTCAGGAG GACGTTTCCG ATGCCCATCT 
TGTAGGCACG AGGTTGTCCT GGACAGGCAT GGTGTCTATG GCCTGCAGCG GAACCTGCTA 
GTGGAGAACA TCATTGACAT CTACAAGCAG GAGTCCTCCC GGCCACTGCA CGCCAAGGCT 
GAACAGCACC TCATGTGTGA GGAGCACGAG GACGAGAAGA TCAACATCTA CTGCCTGAGC 
TGCGAGGTGC CCACCTGCTC TCTCTGCAAG GTTTTCGGCG CCCACAAGGA CTGTGAGGTG 
GCCCCTCTGC CCACCATTTA CAAACGCCAG AAGAGTGAGC TGAGCGATGG CATCGCGATG 
CTGGTGGCGG GCAATGACCG TGTGCAGGCA GTGATCACCC AGATGGAGGA GGTGTGCCAG 
ACCATTGAGG ACAACAGCCG CAGACAGAAG CAACTGTTAA ACCAGAGGTT CGAGACCCTG 
TGCGCGGTTT TGGAGGAGCG CAAGGGCGAA CTGCTTCAAG CACTGGCCCG GGAGCAGGAG 
GAGAAGTTGC AGCGCGTGCG GGGCCTCATC CGCCAGTACG GAGACCACTT GGAGGGCTCC 
TCAAAGCTGG TGGAGTCCGC CATCCAGTCC ATGGAGGAGC CGCAGATGGC TCTCTACCTC 
CAGCAGGCAA AGGAGCTGAT CAACAAGGTC GGGGCAATGT CGAAGGTGGA GCTGGCAGGA 
CGGCCGGAGC CAGGCTATGA GAGCATGGAG CAATTCTCTG TGAGCGTGGA GCACGTGGCC 
GAAATGTTGC GAACCATCGA CTTCCAGCCG GGCGCCGCTG GGGATGAAGA GGATGACGAC 
ATGGCTTTGG ATGGGGAGGA GGGCAATGCG GGGCTGGAGG AGGAGCGGCT GGACGTGCCA 
GAAGGCTCAG GCCTGCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067422.1
CDS	299..1399
Translation	MNFTVGFKPLLGDAHNMDNLEKQLICPICLEMFSKPVVILPCQHNLCRKCANDVFQASNPLWQSRGSTTVSSGGRFRCPSCRHEVVLDRHGVYGLQRNLLVENIIDIYKQESSRPLHAKAEQHLMCEEHEDEKINIYCLSCEVPTCSLCKVFGAHKDCEVAPLPTIYKRQKSELSDGIAMLVAGNDRVQAVITQMEEVCQTIEDNSRRQKQLLNQRFETLCAVLEERKGELLQALAREQEEKLQRVRGLIRQYGDHLEGSSKLVESAIQSMEEPQMALYLQQAKELINKVGAMSKVELAGRPEPGYESMEQFSVSVEHVAEMLRTIDFQPGAAGDEEDDDMALDGEEGNAGLEEERLDVPEGSGLH

Target ORF information:

RefSeq Version	NM_021447.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus tripartite motif-containing 54 (Trim54), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021447.2

ORF Insert Sequence:

ATGAACTTCA CGGTGGGTTT CAAGCCGCTG CTAGGGGATG CGCACAACAT GGACAACTTG 
GAGAAGCAGC TCATTTGCCC CATCTGCCTG GAGATGTTCT CCAAGCCCGT GGTGATCTTG 
CCCTGCCAAC ACAACCTGTG CCGCAAGTGT GCCAACGACG TCTTCCAGGC CTCTAATCCT 
CTGTGGCAAT CCCGGGGCTC CACAACGGTG TCTTCAGGAG GACGTTTCCG ATGCCCATCT 
TGTAGGCACG AGGTTGTCCT GGACAGGCAT GGTGTCTATG GCCTGCAGCG GAACCTGCTA 
GTGGAGAACA TCATTGACAT CTACAAGCAG GAGTCCTCCC GGCCACTGCA CGCCAAGGCT 
GAACAGCACC TCATGTGTGA GGAGCACGAG GACGAGAAGA TCAACATCTA CTGCCTGAGC 
TGCGAGGTGC CCACCTGCTC TCTCTGCAAG GTTTTCGGCG CCCACAAGGA CTGTGAGGTG 
GCCCCTCTGC CCACCATTTA CAAACGCCAG AAGAGTGAGC TGAGCGATGG CATCGCGATG 
CTGGTGGCGG GCAATGACCG TGTGCAGGCA GTGATCACCC AGATGGAGGA GGTGTGCCAG 
ACCATTGAGG ACAACAGCCG CAGACAGAAG CAACTGTTAA ACCAGAGGTT CGAGACCCTG 
TGCGCGGTTT TGGAGGAGCG CAAGGGCGAA CTGCTTCAAG CACTGGCCCG GGAGCAGGAG 
GAGAAGTTGC AGCGCGTGCG GGGCCTCATC CGCCAGTACG GAGACCACTT GGAGGGCTCC 
TCAAAGCTGG TGGAGTCCGC CATCCAGTCC ATGGAGGAGC CGCAGATGGC TCTCTACCTC 
CAGCAGGCAA AGGAGCTGAT CAACAAGGTC GGGGCAATGT CGAAGGTGGA GCTGGCAGGA 
CGGCCGGAGC CAGGCTATGA GAGCATGGAG CAATTCTCTG TGAGCGTGGA GCACGTGGCC 
GAAATGTTGC GAACCATCGA CTTCCAGCCG GGCGCCGCTG GGGATGAAGA GGATGACGAC 
ATGGCTTTGG ATGGGGAGGA GGGCAATGCG GGGCTGGAGG AGGAGCGGCT GGACGTGCCA 
GAAGGCTCAG GCCTGCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Muscle ring finger-3 protects against diabetic cardiomyopathy induced by a high fat diet.
BMC endocrine disorders1536(2015 Jul)
Quintana MT,He J,Sullivan J,Grevengoed T,Schisler J,Han Y,Hill JA,Yates CC,Stansfield WE,Mapanga RF,Essop MF,Muehlbauer MJ,Newgard CB,Bain JR,Willis MS

Developmental regulation of MURF ubiquitin ligases and autophagy proteins nbr1, p62/SQSTM1 and LC3 during cardiac myofibril assembly and turnover.
Developmental biology351(1)46-61(2011 Mar)
Perera S,Holt MR,Mankoo BS,Gautel M

Muscle RING-finger protein-1 (MuRF1) as a connector of muscle energy metabolism and protein synthesis.
Journal of molecular biology376(5)1224-36(2008 Mar)
Koyama S,Hata S,Witt CC,Ono Y,Lerche S,Ojima K,Chiba T,Doi N,Kitamura F,Tanaka K,Abe K,Witt SH,Rybin V,Gasch A,Franz T,Labeit S,Sorimachi H

Myosin accumulation and striated muscle myopathy result from the loss of muscle RING finger 1 and 3.
The Journal of clinical investigation117(9)2486-95(2007 Sep)
Fielitz J,Kim MS,Shelton JM,Latif S,Spencer JA,Glass DJ,Richardson JA,Bassel-Duby R,Olson EN

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Trim54 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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