Rnf25 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rnf25
Entrez Gene ID	57751
Full Name	ring finger protein 25
Synonyms	0610009H16Rik,AO7
General protein information	Preferred Names ring finger protein 25 Names E3 ubiquitin-protein ligase RNF25 RING finger protein AO7 RING-type E3 ubiquitin transferase RNF25
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1\|1 C3

mRNA and Protein(s)

mRNA	Protein	Name
XM_030255695.1	XP_030111555.1	E3 ubiquitin-protein ligase RNF25 isoform X2
NM_001305230.1	NP_001292159.1	E3 ubiquitin-protein ligase RNF25 isoform 2
XM_006496170.1	XP_006496233.1	E3 ubiquitin-protein ligase RNF25 isoform X1
XM_006496170.2	XP_006496233.1	E3 ubiquitin-protein ligase RNF25 isoform X1
NM_021313.3	NP_067288.2	E3 ubiquitin-protein ligase RNF25 isoform 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	RNF25	XP_001158338.1
Homo sapiens (human)	RNF25	NP_071898.2
Canis lupus familiaris (dog)	RNF25	XP_536071.2
Bos taurus (cattle)	RNF25	NP_001015575.1
Mus musculus (house mouse)	Rnf25	NP_067288.2
Rattus norvegicus (Norway rat)	Rnf25	NP_001012004.2
Gallus gallus (chicken)	RNF25	NP_001026430.1
Xenopus tropicalis (tropical clawed frog)	rnf25	XP_002940633.1

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Global gene expression analysis of murine limb development.
Taher L, Collette NM, Murugesh D, Maxwell E, Ovcharenko I, Loots GG
PloS one6(12)e28358(2011)

EGF receptor-independent action of TGF-alpha protects Naked2 from AO7-mediated ubiquitylation and proteasomal degradation.
Ding W, Li C, Hu T, Graves-Deal R, Fotia AB, Weissman AM, Coffey RJ
Proceedings of the National Academy of Sciences of the United States of America105(36)13433-8(2008 Sep)

Construction of a multi-functional cDNA library specific for mouse pancreatic islets and its application to microarray.
Nishimura M, Yokoi N, Miki T, Horikawa Y, Yoshioka H, Takeda J, Ohara O, Seino S
DNA research : an international journal for rapid publication of reports on genes and genomes11(5)315-23(2004 Oct)

RING finger protein AO7 supports NF-kappaB-mediated transcription by interacting with the transactivation domain of the p65 subunit.
Asamitsu K, Tetsuka T, Kanazawa S, Okamoto T
The Journal of biological chemistry278(29)26879-87(2003 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

identify an EGFR-independent action of TGF-alpha, in which it protects Naked2 from proteasomal degradation, thus ensuring its delivery to the basolateral surface of polarized epithelial cells
Title: EGF receptor-independent action of TGF-alpha protects Naked2 from AO7-mediated ubiquitylation and proteasomal degradation.

RING finger protein AO7 supports NF-kappaB-mediated transcription by interacting with the transactivation domain of the p65 subunit.
Title: RING finger protein AO7 supports NF-kappaB-mediated transcription by interacting with the transactivation domain of the p65 subunit.

The following Rnf25 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rnf25 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu12160	NM_021313.3 Latest version!	Mus musculus ring finger protein 25 (Rnf25), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$272.30 $389.00
OMu69746	NM_001305230.1 Latest version!	Mus musculus ring finger protein 25 (Rnf25), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu27548	XM_006496170.2 Latest version!	Mus musculus ring finger protein 25 (Rnf25), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu85233	XM_030255695.1 Latest version!	Mus musculus ring finger protein 25 (Rnf25), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OMu27548	XM_006496170.1	Mus musculus ring finger protein 25 (Rnf25), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rnf25 ( NM_021313.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_067288.2 Mus musculus ring finger protein 25 (Rnf25), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu12160
Clone ID Related Accession (Same CDS sequence)	NM_021313.3
Accession Version	NM_021313.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase RNF25 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK002399.1, BE853767.1, AC117610.7 and BP774070.1. On Mar 5, 2015 this sequence version replaced NM_021313.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: BC048821.1, AF171060.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164132, SAMN01164134 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGT CTGCGTCGAC AGCTGCTGGG GAAGAGGACT GGGTCCTTCC CTCTGAAGTT 
GAAGTATTAG AGTCTATCTA TCTGGATGAG CTACAGGTGA TGAAAGGGAA TGGCAGATCA 
CCATGGGAGA TCTTTATCAC TCTGCACCCT GCCACCGCAG AGGTCCAAGA TTCGCAGTTT 
GTCTGCTTTA CTCTGGTTCT TCGGATCCCA GTCCAGTATC CCCACGAGGT TCCACAGATC 
TCCATCCGTA ACCCTCGAGG ACTTTCAGAT GAGCAGATCC ACAAGATCTC ACAGGCCCTG 
GGCCATGTGG CCAAGGAAGG ACTAGGCACT GCCATGCTCT ATGAACTCAT TGAGAAAGGG 
AAGGAGATTC TCACGGATAA CAACATTCCC CATGGCCAGT GTGTCATCTG CCTCTATGGT 
TTCCAGGAGA AGGAGGCCTT TACCAAAACA CCCTGTTACC ACTACTTCCA CTGCCATTGC 
CTTGCCAGGT ACATCCAGCA CATGGAACAA GAGCTGACTA CACAGGAACA GGAACAGGAG 
CGACAGCACG TTGTCACCAA GCAGAAGGCA GTCGGTGTGC AGTGTCCTGT GTGCAGGGAG 
CCCCTTGTGT ATGATCTTGC CTCGCTGAAA GCAGCCCCTG AACCCCAACA ACCCATGGAG 
CTGTACCAAC CCAGTGCAGA GAGCTTGCGC CAACAGGAAG AGCTCAAACT GCTGTATCAG 
AGGCAGCAGG AAAAGGGTGG TATCATTGAC CTTGAGGCTG AACGGAACCG ATACTTCATT 
AGCCTTCAGC AGCCTCCTGC TGCTCTTGAG CCGGAGTCAG CTGTAGATGT CTCCAGAGAA 
CCCCAGCCAC CTAATGCCCT TTCTGCAGAA CAATCTACTT CCCTAGCTGA CCAATCAACT 
CTGCCAACTT CTCTACCCAT GACAACCCAG TATACGTATG AGAAGACTTC GGGGGCTGGG 
CCAAATCAAC AGAGGCCGGG TGAGACCCAG AAATCCGTGC TAGATCCTCC ACGACATGGT 
CGTGGCTCCT GGCGACAGTA TGATCGGAGG CACCCAAAAG GAGGGGAGTG CTGCACCCCA 
AAAGGTACCA GTGAAATCCA TGAACTGCCA CCTCCTGAGA AGCCTCTCAA GGAGACTGTA 
GACTTAAAGG CAGAACCCCG TAACAAAGGG CTCACAGGTC ATCCTCAGGA GAAGGGGCCT 
GGTAGCTGGC AGGGCCCCTC AGCTCGCAGG ACTCGGGACT GTGCTCGCTG GGAACGCTCC 
AAAAACCGTA CACCGGGTTC TTGCTATCCC CACCTACCTC GGGGCCAGGG AGCATACCGG 
TCTGGTACTC GCAGGGAGCC CCTGGGCTTG GAATCTGAGG AAGGTTCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067288.2
CDS	33..1403
Translation	MAASASTAAGEEDWVLPSEVEVLESIYLDELQVMKGNGRSPWEIFITLHPATAEVQDSQFVCFTLVLRIPVQYPHEVPQISIRNPRGLSDEQIHKISQALGHVAKEGLGTAMLYELIEKGKEILTDNNIPHGQCVICLYGFQEKEAFTKTPCYHYFHCHCLARYIQHMEQELTTQEQEQERQHVVTKQKAVGVQCPVCREPLVYDLASLKAAPEPQQPMELYQPSAESLRQQEELKLLYQRQQEKGGIIDLEAERNRYFISLQQPPAALEPESAVDVSREPQPPNALSAEQSTSLADQSTLPTSLPMTTQYTYEKTSGAGPNQQRPGETQKSVLDPPRHGRGSWRQYDRRHPKGGECCTPKGTSEIHELPPPEKPLKETVDLKAEPRNKGLTGHPQEKGPGSWQGPSARRTRDCARWERSKNRTPGSCYPHLPRGQGAYRSGTRREPLGLESEEGS

Target ORF information:

RefSeq Version	NM_021313.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ring finger protein 25 (Rnf25), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021313.3

ORF Insert Sequence:

ATGGCGGCGT CTGCGTCGAC AGCTGCTGGG GAAGAGGACT GGGTCCTTCC CTCTGAAGTT 
GAAGTATTAG AGTCTATCTA TCTGGATGAG CTACAGGTGA TGAAAGGGAA TGGCAGATCA 
CCATGGGAGA TCTTTATCAC TCTGCACCCT GCCACCGCAG AGGTCCAAGA TTCGCAGTTT 
GTCTGCTTTA CTCTGGTTCT TCGGATCCCA GTCCAGTATC CCCACGAGGT TCCACAGATC 
TCCATCCGTA ACCCTCGAGG ACTTTCAGAT GAGCAGATCC ACAAGATCTC ACAGGCCCTG 
GGCCATGTGG CCAAGGAAGG ACTAGGCACT GCCATGCTCT ATGAACTCAT TGAGAAAGGG 
AAGGAGATTC TCACGGATAA CAACATTCCC CATGGCCAGT GTGTCATCTG CCTCTATGGT 
TTCCAGGAGA AGGAGGCCTT TACCAAAACA CCCTGTTACC ACTACTTCCA CTGCCATTGC 
CTTGCCAGGT ACATCCAGCA CATGGAACAA GAGCTGACTA CACAGGAACA GGAACAGGAG 
CGACAGCACG TTGTCACCAA GCAGAAGGCA GTCGGTGTGC AGTGTCCTGT GTGCAGGGAG 
CCCCTTGTGT ATGATCTTGC CTCGCTGAAA GCAGCCCCTG AACCCCAACA ACCCATGGAG 
CTGTACCAAC CCAGTGCAGA GAGCTTGCGC CAACAGGAAG AGCTCAAACT GCTGTATCAG 
AGGCAGCAGG AAAAGGGTGG TATCATTGAC CTTGAGGCTG AACGGAACCG ATACTTCATT 
AGCCTTCAGC AGCCTCCTGC TGCTCTTGAG CCGGAGTCAG CTGTAGATGT CTCCAGAGAA 
CCCCAGCCAC CTAATGCCCT TTCTGCAGAA CAATCTACTT CCCTAGCTGA CCAATCAACT 
CTGCCAACTT CTCTACCCAT GACAACCCAG TATACGTATG AGAAGACTTC GGGGGCTGGG 
CCAAATCAAC AGAGGCCGGG TGAGACCCAG AAATCCGTGC TAGATCCTCC ACGACATGGT 
CGTGGCTCCT GGCGACAGTA TGATCGGAGG CACCCAAAAG GAGGGGAGTG CTGCACCCCA 
AAAGGTACCA GTGAAATCCA TGAACTGCCA CCTCCTGAGA AGCCTCTCAA GGAGACTGTA 
GACTTAAAGG CAGAACCCCG TAACAAAGGG CTCACAGGTC ATCCTCAGGA GAAGGGGCCT 
GGTAGCTGGC AGGGCCCCTC AGCTCGCAGG ACTCGGGACT GTGCTCGCTG GGAACGCTCC 
AAAAACCGTA CACCGGGTTC TTGCTATCCC CACCTACCTC GGGGCCAGGG AGCATACCGG 
TCTGGTACTC GCAGGGAGCC CCTGGGCTTG GAATCTGAGG AAGGTTCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rnf25 ( NM_001305230.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001292159.1 Mus musculus ring finger protein 25 (Rnf25), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu69746
Clone ID Related Accession (Same CDS sequence)	NM_001305230.1
Accession Version	NM_001305230.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase RNF25 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK002399.1, AK011171.1, BG081026.2, AC117610.7 and BP774070.1. On Mar 5, 2015 this sequence version replaced XM_006496169.2. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region compared to variant (1). The encoded isoform (2) is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC037100.1, SRR1660821.282084.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164132, SAMN01164134 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGT CTGCGTCGAC AGCTGCTGGG GAAGAGGACT GGGTCCTTCC CTCTGAAGTT 
GAAGTATTAG AGTCTATCTA TCTGGATGAG CTACAGGTGA TGAAAGGGAA TGGCAGATCA 
CCATGGGAGA TCTTTATCAC TCTGCACCCT GCCACCGCAG AGGTCCAAGA TTCGCAGTTT 
GTCTGCTTTA CTCTGGTTCT TCGGATCCCA GTCCAGTATC CCCACGAGGT TCCACAGATC 
TCCATCCGTA ACCCTCGAGG ACTTTCAGAT GAGCAGATCC ACAAGATCTC ACAGGCCCTG 
GGCCATGTGG CCAAGGAAGG ACTAGGCACT GCCATGCTCT ATGAACTCAT TGAGAAAGGG 
AAGGAGATTC TCACGGATAA CAACATTCCC CATGGCCAGT GTGTCATCTG CCTCTATGGT 
TTCCAGGAGA AGGAGGCCTT TACCAAAACA CCCTGTTACC ACTACTTCCA CTGCCATTGC 
CTTGCCAGGT ACATCCAGCA CATGGAACAA GAGCTGACTA CACAGGAACA GGAACAGGAG 
CGACAGCACG TTGTCACCAA GCAGGCAGTC GGTGTGCAGT GTCCTGTGTG CAGGGAGCCC 
CTTGTGTATG ATCTTGCCTC GCTGAAAGCA GCCCCTGAAC CCCAACAACC CATGGAGCTG 
TACCAACCCA GTGCAGAGAG CTTGCGCCAA CAGGAAGAGC TCAAACTGCT GTATCAGAGG 
CAGCAGGAAA AGGGTGGTAT CATTGACCTT GAGGCTGAAC GGAACCGATA CTTCATTAGC 
CTTCAGCAGC CTCCTGCTGC TCTTGAGCCG GAGTCAGCTG TAGATGTCTC CAGAGAACCC 
CAGCCACCTA ATGCCCTTTC TGCAGAACAA TCTACTTCCC TAGCTGACCA ATCAACTCTG 
CCAACTTCTC TACCCATGAC AACCCAGTAT ACGTATGAGA AGACTTCGGG GGCTGGGCCA 
AATCAACAGA GGCCGGGTGA GACCCAGAAA TCCGTGCTAG ATCCTCCACG ACATGGTCGT 
GGCTCCTGGC GACAGTATGA TCGGAGGCAC CCAAAAGGAG GGGAGTGCTG CACCCCAAAA 
GGTACCAGTG AAATCCATGA ACTGCCACCT CCTGAGAAGC CTCTCAAGGA GACTGTAGAC 
TTAAAGGCAG AACCCCGTAA CAAAGGGCTC ACAGGTCATC CTCAGGAGAA GGGGCCTGGT 
AGCTGGCAGG GCCCCTCAGC TCGCAGGACT CGGGACTGTG CTCGCTGGGA ACGCTCCAAA 
AACCGTACAC CGGGTTCTTG CTATCCCCAC CTACCTCGGG GCCAGGGAGC ATACCGGTCT 
GGTACTCGCA GGGAGCCCCT GGGCTTGGAA TCTGAGGAAG GTTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001292159.1
CDS	33..1400
Translation	MAASASTAAGEEDWVLPSEVEVLESIYLDELQVMKGNGRSPWEIFITLHPATAEVQDSQFVCFTLVLRIPVQYPHEVPQISIRNPRGLSDEQIHKISQALGHVAKEGLGTAMLYELIEKGKEILTDNNIPHGQCVICLYGFQEKEAFTKTPCYHYFHCHCLARYIQHMEQELTTQEQEQERQHVVTKQAVGVQCPVCREPLVYDLASLKAAPEPQQPMELYQPSAESLRQQEELKLLYQRQQEKGGIIDLEAERNRYFISLQQPPAALEPESAVDVSREPQPPNALSAEQSTSLADQSTLPTSLPMTTQYTYEKTSGAGPNQQRPGETQKSVLDPPRHGRGSWRQYDRRHPKGGECCTPKGTSEIHELPPPEKPLKETVDLKAEPRNKGLTGHPQEKGPGSWQGPSARRTRDCARWERSKNRTPGSCYPHLPRGQGAYRSGTRREPLGLESEEGS

Target ORF information:

RefSeq Version	NM_001305230.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ring finger protein 25 (Rnf25), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001305230.1

ORF Insert Sequence:

ATGGCGGCGT CTGCGTCGAC AGCTGCTGGG GAAGAGGACT GGGTCCTTCC CTCTGAAGTT 
GAAGTATTAG AGTCTATCTA TCTGGATGAG CTACAGGTGA TGAAAGGGAA TGGCAGATCA 
CCATGGGAGA TCTTTATCAC TCTGCACCCT GCCACCGCAG AGGTCCAAGA TTCGCAGTTT 
GTCTGCTTTA CTCTGGTTCT TCGGATCCCA GTCCAGTATC CCCACGAGGT TCCACAGATC 
TCCATCCGTA ACCCTCGAGG ACTTTCAGAT GAGCAGATCC ACAAGATCTC ACAGGCCCTG 
GGCCATGTGG CCAAGGAAGG ACTAGGCACT GCCATGCTCT ATGAACTCAT TGAGAAAGGG 
AAGGAGATTC TCACGGATAA CAACATTCCC CATGGCCAGT GTGTCATCTG CCTCTATGGT 
TTCCAGGAGA AGGAGGCCTT TACCAAAACA CCCTGTTACC ACTACTTCCA CTGCCATTGC 
CTTGCCAGGT ACATCCAGCA CATGGAACAA GAGCTGACTA CACAGGAACA GGAACAGGAG 
CGACAGCACG TTGTCACCAA GCAGGCAGTC GGTGTGCAGT GTCCTGTGTG CAGGGAGCCC 
CTTGTGTATG ATCTTGCCTC GCTGAAAGCA GCCCCTGAAC CCCAACAACC CATGGAGCTG 
TACCAACCCA GTGCAGAGAG CTTGCGCCAA CAGGAAGAGC TCAAACTGCT GTATCAGAGG 
CAGCAGGAAA AGGGTGGTAT CATTGACCTT GAGGCTGAAC GGAACCGATA CTTCATTAGC 
CTTCAGCAGC CTCCTGCTGC TCTTGAGCCG GAGTCAGCTG TAGATGTCTC CAGAGAACCC 
CAGCCACCTA ATGCCCTTTC TGCAGAACAA TCTACTTCCC TAGCTGACCA ATCAACTCTG 
CCAACTTCTC TACCCATGAC AACCCAGTAT ACGTATGAGA AGACTTCGGG GGCTGGGCCA 
AATCAACAGA GGCCGGGTGA GACCCAGAAA TCCGTGCTAG ATCCTCCACG ACATGGTCGT 
GGCTCCTGGC GACAGTATGA TCGGAGGCAC CCAAAAGGAG GGGAGTGCTG CACCCCAAAA 
GGTACCAGTG AAATCCATGA ACTGCCACCT CCTGAGAAGC CTCTCAAGGA GACTGTAGAC 
TTAAAGGCAG AACCCCGTAA CAAAGGGCTC ACAGGTCATC CTCAGGAGAA GGGGCCTGGT 
AGCTGGCAGG GCCCCTCAGC TCGCAGGACT CGGGACTGTG CTCGCTGGGA ACGCTCCAAA 
AACCGTACAC CGGGTTCTTG CTATCCCCAC CTACCTCGGG GCCAGGGAGC ATACCGGTCT 
GGTACTCGCA GGGAGCCCCT GGGCTTGGAA TCTGAGGAAG GTTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rnf25 ( XM_006496170.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496233.1 Mus musculus ring finger protein 25 (Rnf25), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu27548
Clone ID Related Accession (Same CDS sequence)	XM_006496170.1 , XM_006496170.2
Accession Version	XM_006496170.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1272bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase RNF25 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGGGA ATGGCAGATC ACCATGGGAG ATCTTTATCA CTCTGCACCC TGCCACCGCA 
GAGGTCCAAG ATTCGCAGTT TGTCTGCTTT ACTCTGGTTC TTCGGATCCC AGTCCAGTAT 
CCCCACGAGG TTCCACAGAT CTCCATCCGT AACCCTCGAG GACTTTCAGA TGAGCAGATC 
CACAAGATCT CACAGGCCCT GGGCCATGTG GCCAAGGAAG GACTAGGCAC TGCCATGCTC 
TATGAACTCA TTGAGAAAGG GAAGGAGATT CTCACGGATA ACAACATTCC CCATGGCCAG 
TGTGTCATCT GCCTCTATGG TTTCCAGGAG AAGGAGGCCT TTACCAAAAC ACCCTGTTAC 
CACTACTTCC ACTGCCATTG CCTTGCCAGG TACATCCAGC ACATGGAACA AGAGCTGACT 
ACACAGGAAC AGGAACAGGA GCGACAGCAC GTTGTCACCA AGCAGAAGGC AGTCGGTGTG 
CAGTGTCCTG TGTGCAGGGA GCCCCTTGTG TATGATCTTG CCTCGCTGAA AGCAGCCCCT 
GAACCCCAAC AACCCATGGA GCTGTACCAA CCCAGTGCAG AGAGCTTGCG CCAACAGGAA 
GAGCTCAAAC TGCTGTATCA GAGGCAGCAG GAAAAGGGTG GTATCATTGA CCTTGAGGCT 
GAACGGAACC GATACTTCAT TAGCCTTCAG CAGCCTCCTG CTGCTCTTGA GCCGGAGTCA 
GCTGTAGATG TCTCCAGAGA ACCCCAGCCA CCTAATGCCC TTTCTGCAGA ACAATCTACT 
TCCCTAGCTG ACCAATCAAC TCTGCCAACT TCTCTACCCA TGACAACCCA GTATACGTAT 
GAGAAGACTT CGGGGGCTGG GCCAAATCAA CAGAGGCCGG GTGAGACCCA GAAATCCGTG 
CTAGATCCTC CACGACATGG TCGTGGCTCC TGGCGACAGT ATGATCGGAG GCACCCAAAA 
GGAGGGGAGT GCTGCACCCC AAAAGGTACC AGTGAAATCC ATGAACTGCC ACCTCCTGAG 
AAGCCTCTCA AGGAGACTGT AGACTTAAAG GCAGAACCCC GTAACAAAGG GCTCACAGGT 
CATCCTCAGG AGAAGGGGCC TGGTAGCTGG CAGGGCCCCT CAGCTCGCAG GACTCGGGAC 
TGTGCTCGCT GGGAACGCTC CAAAAACCGT ACACCGGGTT CTTGCTATCC CCACCTACCT 
CGGGGCCAGG GAGCATACCG GTCTGGTACT CGCAGGGAGC CCCTGGGCTT GGAATCTGAG 
GAAGGTTCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496233.1
CDS	896..2167
Translation	MKGNGRSPWEIFITLHPATAEVQDSQFVCFTLVLRIPVQYPHEVPQISIRNPRGLSDEQIHKISQALGHVAKEGLGTAMLYELIEKGKEILTDNNIPHGQCVICLYGFQEKEAFTKTPCYHYFHCHCLARYIQHMEQELTTQEQEQERQHVVTKQKAVGVQCPVCREPLVYDLASLKAAPEPQQPMELYQPSAESLRQQEELKLLYQRQQEKGGIIDLEAERNRYFISLQQPPAALEPESAVDVSREPQPPNALSAEQSTSLADQSTLPTSLPMTTQYTYEKTSGAGPNQQRPGETQKSVLDPPRHGRGSWRQYDRRHPKGGECCTPKGTSEIHELPPPEKPLKETVDLKAEPRNKGLTGHPQEKGPGSWQGPSARRTRDCARWERSKNRTPGSCYPHLPRGQGAYRSGTRREPLGLESEEGS

Target ORF information:

RefSeq Version	XM_006496170.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ring finger protein 25 (Rnf25), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496170.1

ORF Insert Sequence:

ATGAAAGGGA ATGGCAGATC ACCATGGGAG ATCTTTATCA CTCTGCACCC TGCCACCGCA 
GAGGTCCAAG ATTCGCAGTT TGTCTGCTTT ACTCTGGTTC TTCGGATCCC AGTCCAGTAT 
CCCCACGAGG TTCCACAGAT CTCCATCCGT AACCCTCGAG GACTTTCAGA TGAGCAGATC 
CACAAGATCT CACAGGCCCT GGGCCATGTG GCCAAGGAAG GACTAGGCAC TGCCATGCTC 
TATGAACTCA TTGAGAAAGG GAAGGAGATT CTCACGGATA ACAACATTCC CCATGGCCAG 
TGTGTCATCT GCCTCTATGG TTTCCAGGAG AAGGAGGCCT TTACCAAAAC ACCCTGTTAC 
CACTACTTCC ACTGCCATTG CCTTGCCAGG TACATCCAGC ACATGGAACA AGAGCTGACT 
ACACAGGAAC AGGAACAGGA GCGACAGCAC GTTGTCACCA AGCAGAAGGC AGTCGGTGTG 
CAGTGTCCTG TGTGCAGGGA GCCCCTTGTG TATGATCTTG CCTCGCTGAA AGCAGCCCCT 
GAACCCCAAC AACCCATGGA GCTGTACCAA CCCAGTGCAG AGAGCTTGCG CCAACAGGAA 
GAGCTCAAAC TGCTGTATCA GAGGCAGCAG GAAAAGGGTG GTATCATTGA CCTTGAGGCT 
GAACGGAACC GATACTTCAT TAGCCTTCAG CAGCCTCCTG CTGCTCTTGA GCCGGAGTCA 
GCTGTAGATG TCTCCAGAGA ACCCCAGCCA CCTAATGCCC TTTCTGCAGA ACAATCTACT 
TCCCTAGCTG ACCAATCAAC TCTGCCAACT TCTCTACCCA TGACAACCCA GTATACGTAT 
GAGAAGACTT CGGGGGCTGG GCCAAATCAA CAGAGGCCGG GTGAGACCCA GAAATCCGTG 
CTAGATCCTC CACGACATGG TCGTGGCTCC TGGCGACAGT ATGATCGGAG GCACCCAAAA 
GGAGGGGAGT GCTGCACCCC AAAAGGTACC AGTGAAATCC ATGAACTGCC ACCTCCTGAG 
AAGCCTCTCA AGGAGACTGT AGACTTAAAG GCAGAACCCC GTAACAAAGG GCTCACAGGT 
CATCCTCAGG AGAAGGGGCC TGGTAGCTGG CAGGGCCCCT CAGCTCGCAG GACTCGGGAC 
TGTGCTCGCT GGGAACGCTC CAAAAACCGT ACACCGGGTT CTTGCTATCC CCACCTACCT 
CGGGGCCAGG GAGCATACCG GTCTGGTACT CGCAGGGAGC CCCTGGGCTT GGAATCTGAG 
GAAGGTTCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rnf25 ( XM_006496170.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496233.1 Mus musculus ring finger protein 25 (Rnf25), transcript variant X1, mRNA.

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CloneID	OMu27548
Clone ID Related Accession (Same CDS sequence)	XM_006496170.1 , XM_006496170.2
Accession Version	XM_006496170.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1272bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase RNF25 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006496170.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGGGA ATGGCAGATC ACCATGGGAG ATCTTTATCA CTCTGCACCC TGCCACCGCA 
GAGGTCCAAG ATTCGCAGTT TGTCTGCTTT ACTCTGGTTC TTCGGATCCC AGTCCAGTAT 
CCCCACGAGG TTCCACAGAT CTCCATCCGT AACCCTCGAG GACTTTCAGA TGAGCAGATC 
CACAAGATCT CACAGGCCCT GGGCCATGTG GCCAAGGAAG GACTAGGCAC TGCCATGCTC 
TATGAACTCA TTGAGAAAGG GAAGGAGATT CTCACGGATA ACAACATTCC CCATGGCCAG 
TGTGTCATCT GCCTCTATGG TTTCCAGGAG AAGGAGGCCT TTACCAAAAC ACCCTGTTAC 
CACTACTTCC ACTGCCATTG CCTTGCCAGG TACATCCAGC ACATGGAACA AGAGCTGACT 
ACACAGGAAC AGGAACAGGA GCGACAGCAC GTTGTCACCA AGCAGAAGGC AGTCGGTGTG 
CAGTGTCCTG TGTGCAGGGA GCCCCTTGTG TATGATCTTG CCTCGCTGAA AGCAGCCCCT 
GAACCCCAAC AACCCATGGA GCTGTACCAA CCCAGTGCAG AGAGCTTGCG CCAACAGGAA 
GAGCTCAAAC TGCTGTATCA GAGGCAGCAG GAAAAGGGTG GTATCATTGA CCTTGAGGCT 
GAACGGAACC GATACTTCAT TAGCCTTCAG CAGCCTCCTG CTGCTCTTGA GCCGGAGTCA 
GCTGTAGATG TCTCCAGAGA ACCCCAGCCA CCTAATGCCC TTTCTGCAGA ACAATCTACT 
TCCCTAGCTG ACCAATCAAC TCTGCCAACT TCTCTACCCA TGACAACCCA GTATACGTAT 
GAGAAGACTT CGGGGGCTGG GCCAAATCAA CAGAGGCCGG GTGAGACCCA GAAATCCGTG 
CTAGATCCTC CACGACATGG TCGTGGCTCC TGGCGACAGT ATGATCGGAG GCACCCAAAA 
GGAGGGGAGT GCTGCACCCC AAAAGGTACC AGTGAAATCC ATGAACTGCC ACCTCCTGAG 
AAGCCTCTCA AGGAGACTGT AGACTTAAAG GCAGAACCCC GTAACAAAGG GCTCACAGGT 
CATCCTCAGG AGAAGGGGCC TGGTAGCTGG CAGGGCCCCT CAGCTCGCAG GACTCGGGAC 
TGTGCTCGCT GGGAACGCTC CAAAAACCGT ACACCGGGTT CTTGCTATCC CCACCTACCT 
CGGGGCCAGG GAGCATACCG GTCTGGTACT CGCAGGGAGC CCCTGGGCTT GGAATCTGAG 
GAAGGTTCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496233.1
CDS	158..1429
Translation	MKGNGRSPWEIFITLHPATAEVQDSQFVCFTLVLRIPVQYPHEVPQISIRNPRGLSDEQIHKISQALGHVAKEGLGTAMLYELIEKGKEILTDNNIPHGQCVICLYGFQEKEAFTKTPCYHYFHCHCLARYIQHMEQELTTQEQEQERQHVVTKQKAVGVQCPVCREPLVYDLASLKAAPEPQQPMELYQPSAESLRQQEELKLLYQRQQEKGGIIDLEAERNRYFISLQQPPAALEPESAVDVSREPQPPNALSAEQSTSLADQSTLPTSLPMTTQYTYEKTSGAGPNQQRPGETQKSVLDPPRHGRGSWRQYDRRHPKGGECCTPKGTSEIHELPPPEKPLKETVDLKAEPRNKGLTGHPQEKGPGSWQGPSARRTRDCARWERSKNRTPGSCYPHLPRGQGAYRSGTRREPLGLESEEGS

Target ORF information:

RefSeq Version	XM_006496170.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ring finger protein 25 (Rnf25), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496170.2

ORF Insert Sequence:

ATGAAAGGGA ATGGCAGATC ACCATGGGAG ATCTTTATCA CTCTGCACCC TGCCACCGCA 
GAGGTCCAAG ATTCGCAGTT TGTCTGCTTT ACTCTGGTTC TTCGGATCCC AGTCCAGTAT 
CCCCACGAGG TTCCACAGAT CTCCATCCGT AACCCTCGAG GACTTTCAGA TGAGCAGATC 
CACAAGATCT CACAGGCCCT GGGCCATGTG GCCAAGGAAG GACTAGGCAC TGCCATGCTC 
TATGAACTCA TTGAGAAAGG GAAGGAGATT CTCACGGATA ACAACATTCC CCATGGCCAG 
TGTGTCATCT GCCTCTATGG TTTCCAGGAG AAGGAGGCCT TTACCAAAAC ACCCTGTTAC 
CACTACTTCC ACTGCCATTG CCTTGCCAGG TACATCCAGC ACATGGAACA AGAGCTGACT 
ACACAGGAAC AGGAACAGGA GCGACAGCAC GTTGTCACCA AGCAGAAGGC AGTCGGTGTG 
CAGTGTCCTG TGTGCAGGGA GCCCCTTGTG TATGATCTTG CCTCGCTGAA AGCAGCCCCT 
GAACCCCAAC AACCCATGGA GCTGTACCAA CCCAGTGCAG AGAGCTTGCG CCAACAGGAA 
GAGCTCAAAC TGCTGTATCA GAGGCAGCAG GAAAAGGGTG GTATCATTGA CCTTGAGGCT 
GAACGGAACC GATACTTCAT TAGCCTTCAG CAGCCTCCTG CTGCTCTTGA GCCGGAGTCA 
GCTGTAGATG TCTCCAGAGA ACCCCAGCCA CCTAATGCCC TTTCTGCAGA ACAATCTACT 
TCCCTAGCTG ACCAATCAAC TCTGCCAACT TCTCTACCCA TGACAACCCA GTATACGTAT 
GAGAAGACTT CGGGGGCTGG GCCAAATCAA CAGAGGCCGG GTGAGACCCA GAAATCCGTG 
CTAGATCCTC CACGACATGG TCGTGGCTCC TGGCGACAGT ATGATCGGAG GCACCCAAAA 
GGAGGGGAGT GCTGCACCCC AAAAGGTACC AGTGAAATCC ATGAACTGCC ACCTCCTGAG 
AAGCCTCTCA AGGAGACTGT AGACTTAAAG GCAGAACCCC GTAACAAAGG GCTCACAGGT 
CATCCTCAGG AGAAGGGGCC TGGTAGCTGG CAGGGCCCCT CAGCTCGCAG GACTCGGGAC 
TGTGCTCGCT GGGAACGCTC CAAAAACCGT ACACCGGGTT CTTGCTATCC CCACCTACCT 
CGGGGCCAGG GAGCATACCG GTCTGGTACT CGCAGGGAGC CCCTGGGCTT GGAATCTGAG 
GAAGGTTCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rnf25 ( XM_030255695.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030111555.1 Mus musculus ring finger protein 25 (Rnf25), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu85233
Clone ID Related Accession (Same CDS sequence)	XM_030255695.1
Accession Version	XM_030255695.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1269bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase RNF25 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGGGA ATGGCAGATC ACCATGGGAG ATCTTTATCA CTCTGCACCC TGCCACCGCA 
GAGGTCCAAG ATTCGCAGTT TGTCTGCTTT ACTCTGGTTC TTCGGATCCC AGTCCAGTAT 
CCCCACGAGG TTCCACAGAT CTCCATCCGT AACCCTCGAG GACTTTCAGA TGAGCAGATC 
CACAAGATCT CACAGGCCCT GGGCCATGTG GCCAAGGAAG GACTAGGCAC TGCCATGCTC 
TATGAACTCA TTGAGAAAGG GAAGGAGATT CTCACGGATA ACAACATTCC CCATGGCCAG 
TGTGTCATCT GCCTCTATGG TTTCCAGGAG AAGGAGGCCT TTACCAAAAC ACCCTGTTAC 
CACTACTTCC ACTGCCATTG CCTTGCCAGG TACATCCAGC ACATGGAACA AGAGCTGACT 
ACACAGGAAC AGGAACAGGA GCGACAGCAC GTTGTCACCA AGCAGGCAGT CGGTGTGCAG 
TGTCCTGTGT GCAGGGAGCC CCTTGTGTAT GATCTTGCCT CGCTGAAAGC AGCCCCTGAA 
CCCCAACAAC CCATGGAGCT GTACCAACCC AGTGCAGAGA GCTTGCGCCA ACAGGAAGAG 
CTCAAACTGC TGTATCAGAG GCAGCAGGAA AAGGGTGGTA TCATTGACCT TGAGGCTGAA 
CGGAACCGAT ACTTCATTAG CCTTCAGCAG CCTCCTGCTG CTCTTGAGCC GGAGTCAGCT 
GTAGATGTCT CCAGAGAACC CCAGCCACCT AATGCCCTTT CTGCAGAACA ATCTACTTCC 
CTAGCTGACC AATCAACTCT GCCAACTTCT CTACCCATGA CAACCCAGTA TACGTATGAG 
AAGACTTCGG GGGCTGGGCC AAATCAACAG AGGCCGGGTG AGACCCAGAA ATCCGTGCTA 
GATCCTCCAC GACATGGTCG TGGCTCCTGG CGACAGTATG ATCGGAGGCA CCCAAAAGGA 
GGGGAGTGCT GCACCCCAAA AGGTACCAGT GAAATCCATG AACTGCCACC TCCTGAGAAG 
CCTCTCAAGG AGACTGTAGA CTTAAAGGCA GAACCCCGTA ACAAAGGGCT CACAGGTCAT 
CCTCAGGAGA AGGGGCCTGG TAGCTGGCAG GGCCCCTCAG CTCGCAGGAC TCGGGACTGT 
GCTCGCTGGG AACGCTCCAA AAACCGTACA CCGGGTTCTT GCTATCCCCA CCTACCTCGG 
GGCCAGGGAG CATACCGGTC TGGTACTCGC AGGGAGCCCC TGGGCTTGGA ATCTGAGGAA 
GGTTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030111555.1
CDS	158..1426
Translation	MKGNGRSPWEIFITLHPATAEVQDSQFVCFTLVLRIPVQYPHEVPQISIRNPRGLSDEQIHKISQALGHVAKEGLGTAMLYELIEKGKEILTDNNIPHGQCVICLYGFQEKEAFTKTPCYHYFHCHCLARYIQHMEQELTTQEQEQERQHVVTKQAVGVQCPVCREPLVYDLASLKAAPEPQQPMELYQPSAESLRQQEELKLLYQRQQEKGGIIDLEAERNRYFISLQQPPAALEPESAVDVSREPQPPNALSAEQSTSLADQSTLPTSLPMTTQYTYEKTSGAGPNQQRPGETQKSVLDPPRHGRGSWRQYDRRHPKGGECCTPKGTSEIHELPPPEKPLKETVDLKAEPRNKGLTGHPQEKGPGSWQGPSARRTRDCARWERSKNRTPGSCYPHLPRGQGAYRSGTRREPLGLESEEGS

Target ORF information:

RefSeq Version	XM_030255695.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ring finger protein 25 (Rnf25), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030255695.1

ORF Insert Sequence:

ATGAAAGGGA ATGGCAGATC ACCATGGGAG ATCTTTATCA CTCTGCACCC TGCCACCGCA 
GAGGTCCAAG ATTCGCAGTT TGTCTGCTTT ACTCTGGTTC TTCGGATCCC AGTCCAGTAT 
CCCCACGAGG TTCCACAGAT CTCCATCCGT AACCCTCGAG GACTTTCAGA TGAGCAGATC 
CACAAGATCT CACAGGCCCT GGGCCATGTG GCCAAGGAAG GACTAGGCAC TGCCATGCTC 
TATGAACTCA TTGAGAAAGG GAAGGAGATT CTCACGGATA ACAACATTCC CCATGGCCAG 
TGTGTCATCT GCCTCTATGG TTTCCAGGAG AAGGAGGCCT TTACCAAAAC ACCCTGTTAC 
CACTACTTCC ACTGCCATTG CCTTGCCAGG TACATCCAGC ACATGGAACA AGAGCTGACT 
ACACAGGAAC AGGAACAGGA GCGACAGCAC GTTGTCACCA AGCAGGCAGT CGGTGTGCAG 
TGTCCTGTGT GCAGGGAGCC CCTTGTGTAT GATCTTGCCT CGCTGAAAGC AGCCCCTGAA 
CCCCAACAAC CCATGGAGCT GTACCAACCC AGTGCAGAGA GCTTGCGCCA ACAGGAAGAG 
CTCAAACTGC TGTATCAGAG GCAGCAGGAA AAGGGTGGTA TCATTGACCT TGAGGCTGAA 
CGGAACCGAT ACTTCATTAG CCTTCAGCAG CCTCCTGCTG CTCTTGAGCC GGAGTCAGCT 
GTAGATGTCT CCAGAGAACC CCAGCCACCT AATGCCCTTT CTGCAGAACA ATCTACTTCC 
CTAGCTGACC AATCAACTCT GCCAACTTCT CTACCCATGA CAACCCAGTA TACGTATGAG 
AAGACTTCGG GGGCTGGGCC AAATCAACAG AGGCCGGGTG AGACCCAGAA ATCCGTGCTA 
GATCCTCCAC GACATGGTCG TGGCTCCTGG CGACAGTATG ATCGGAGGCA CCCAAAAGGA 
GGGGAGTGCT GCACCCCAAA AGGTACCAGT GAAATCCATG AACTGCCACC TCCTGAGAAG 
CCTCTCAAGG AGACTGTAGA CTTAAAGGCA GAACCCCGTA ACAAAGGGCT CACAGGTCAT 
CCTCAGGAGA AGGGGCCTGG TAGCTGGCAG GGCCCCTCAG CTCGCAGGAC TCGGGACTGT 
GCTCGCTGGG AACGCTCCAA AAACCGTACA CCGGGTTCTT GCTATCCCCA CCTACCTCGG 
GGCCAGGGAG CATACCGGTC TGGTACTCGC AGGGAGCCCC TGGGCTTGGA ATCTGAGGAA 
GGTTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Global gene expression analysis of murine limb development.
PloS one6(12)e28358(2011)
Taher L,Collette NM,Murugesh D,Maxwell E,Ovcharenko I,Loots GG

EGF receptor-independent action of TGF-alpha protects Naked2 from AO7-mediated ubiquitylation and proteasomal degradation.
Proceedings of the National Academy of Sciences of the United States of America105(36)13433-8(2008 Sep)
Ding W,Li C,Hu T,Graves-Deal R,Fotia AB,Weissman AM,Coffey RJ

Construction of a multi-functional cDNA library specific for mouse pancreatic islets and its application to microarray.
DNA research : an international journal for rapid publication of reports on genes and genomes11(5)315-23(2004 Oct)
Nishimura M,Yokoi N,Miki T,Horikawa Y,Yoshioka H,Takeda J,Ohara O,Seino S

RING finger protein AO7 supports NF-kappaB-mediated transcription by interacting with the transactivation domain of the p65 subunit.
The Journal of biological chemistry278(29)26879-87(2003 Jul)
Asamitsu K,Tetsuka T,Kanazawa S,Okamoto T

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Rnf25 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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