Srd5a3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Srd5a3
Entrez Gene ID	57357
Full Name	steroid 5 alpha-reductase 3
Synonyms	1110025P14Rik,A430076C09,AV364670,AW987574,D730040M03Rik,H5ar,S5AR 3,Srd5a2l
General protein information	Preferred Names steroid 5 alpha-reductase 3 Names polyprenol reductase 3-oxo-5-alpha-steroid 4-dehydrogenase 3 H5AR SR type 3 probable polyprenol reductase steroid 5 alpha-reductase 2 like steroid 5 alpha-reductase 2-like; H5AR gene; steroid 5 alpha-reductase 2 like
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 C3.3

mRNA and Protein(s)

mRNA	Protein	Name
XM_006535162.1	XP_006535225.1	polyprenol reductase isoform X1
NM_020611.4	NP_065636.2	polyprenol reductase

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SRD5A3	XP_526623.1
Canis lupus familiaris (dog)	SRD5A3	XP_539274.2
Mus musculus (house mouse)	Srd5a3	NP_065636.2
Gallus gallus (chicken)	SRD5A3	XP_420703.2
Homo sapiens (human)	SRD5A3	NP_078868.1
	SRD5A3	XP_001088853.1
Rattus norvegicus (Norway rat)	Srd5a3	NP_001014012.1
Caenorhabditis elegans (roundworm)	B0024.13	NP_001256209.1
Bos taurus (cattle)	SRD5A3	NP_001193019.1
Danio rerio (zebrafish)	srd5a3	NP_001038404.1
Drosophila melanogaster (fruit fly)	CG7840	NP_609203.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007493	XP_308382.3
Xenopus tropicalis (tropical clawed frog)	srd5a3	NP_001072539.1

Gene Ontology
Bibliography

Related articles in PubMed

Systematic screening for skin, hair, and nail abnormalities in a large-scale knockout mouse program.
Sundberg JP, Dadras SS, Silva KA, Kennedy VE, Garland G, Murray SA, Sundberg BA, Schofield PN, Pratt CH
PloS one12(7)e0180682(2017)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Peri-implantation lethality in mice carrying megabase-scale deletion on 5qc3.3 is caused by Exoc1 null mutation.
Mizuno S, Takami K, Daitoku Y, Tanimoto Y, Dinh TT, Mizuno-Iijima S, Hasegawa Y, Takahashi S, Sugiyama F, Yagami K
Scientific reports513632(2015 Sep)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Srd5a3 mutation is lethal in mouse and results in an activation of the unfolded protein response pathway.
Title: SRD5A3 is required for converting polyprenol to dolichol and is mutated in a congenital glycosylation disorder.

The following Srd5a3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Srd5a3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu12068	NM_020611.4 Latest version!	Mus musculus steroid 5 alpha-reductase 3 (Srd5a3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu35629	XM_006535162.1 Latest version!	Mus musculus steroid 5 alpha-reductase 3 (Srd5a3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Srd5a3 ( NM_020611.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_065636.2 Mus musculus steroid 5 alpha-reductase 3 (Srd5a3), mRNA.

Price & Availability↑

CloneID	OMu12068
Clone ID Related Accession (Same CDS sequence)	NM_020611.4
Accession Version	NM_020611.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	polyprenol reductase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AI553072.1, AK075635.1 and AK164282.1. On Mar 23, 2011 this sequence version replaced NM_020611.3. ##Evidence-Data-START## Transcript exon combination :: AK075635.1, AK162527.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGGGT GGGCCGGGTT TGAGCTCTCG GCCCTGAACC CCCTGCGGAC GCTGTGGCTG 
GCGCTGGCCG CCGCCTTCCT GTTCGCGCTG CTGCTGCAGC TGGCGCCCGC CAGGCTGCTG 
CCGAGCTGCG CGCTCTTCCA GGACCTGCTC CGCTACGGGA AGACCAAGCA GTCCGGCTCG 
CGGCGCCCCG CCGTCTGCAG GGCCTTCGAT GTCCCCAAGA GGTACTTTTC TCACTTCTAC 
GTCATCTCAG TTGTGTGGAA TGGCTCCCTG CTCTGGCTAC TTTCTCAGTC GTTGTTCCTG 
GGAGCACCTT TTCCAAACTG GCTTAGTGCT CTGCTCAGAA CTCTTGGGGC CACACAGTTC 
CAAGCCCTGG AGATGGAGTC CAAGGCTTCT CGGATGCCAG CGGCTGAGCT GGCTCTGTCT 
GCCTTCTTGG TCTTGGTGTT CCTCTGGGTC CACAGCCTTC GGAGACTCTT TGAGTGCTTC 
TACGTCAGTG TCTTCTCTAA TGCAGCCATT CACGTTGTGC AGTACTGTTT CGGGCTTGTC 
TACTATGTGC TTGTTGGCCT GACTGTACTG AGCCAAGTGC CCATGGATGA TAAGAATGTG 
TATGTTCTGG GGAAGAATCT ACTGATACAA GCCCGGTGGT TCCACATCCT GGGCATGGTG 
ATGTTCTTCT GGTCATCCGC CCATCAGTAT AAATGCCACG TCATCCTCAG CAACCTCAGG 
AGAAACAAAA AAGGTGTGGT CATCCACTGC CAGCACCGGA TCCCCTTTGG AGACTGGTTC 
GAGTACGTGT CTTCAGCTAA CTACCTAGCA GAGCTGATGA TCTACATCTC CATGGCTGTC 
ACCTTTGGGC TCCACAACTT AACCTGGTGG CTGGTGGTGA CGTATGTCTT CTCCAGCCAA 
GCCTTGTCCG CATTCTTCAA CCACAAGTTC TACAGAAGCA CATTTGTCTC CTACCCAAAG 
CATAGGAAAG CGTTCCTCCC ATTTTTGTTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065636.2
CDS	56..1048
Translation	MAGWAGFELSALNPLRTLWLALAAAFLFALLLQLAPARLLPSCALFQDLLRYGKTKQSGSRRPAVCRAFDVPKRYFSHFYVISVVWNGSLLWLLSQSLFLGAPFPNWLSALLRTLGATQFQALEMESKASRMPAAELALSAFLVLVFLWVHSLRRLFECFYVSVFSNAAIHVVQYCFGLVYYVLVGLTVLSQVPMDDKNVYVLGKNLLIQARWFHILGMVMFFWSSAHQYKCHVILSNLRRNKKGVVIHCQHRIPFGDWFEYVSSANYLAELMIYISMAVTFGLHNLTWWLVVTYVFSSQALSAFFNHKFYRSTFVSYPKHRKAFLPFLF

Target ORF information:

RefSeq Version	NM_020611.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus steroid 5 alpha-reductase 3 (Srd5a3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020611.4

ORF Insert Sequence:

ATGGCTGGGT GGGCCGGGTT TGAGCTCTCG GCCCTGAACC CCCTGCGGAC GCTGTGGCTG 
GCGCTGGCCG CCGCCTTCCT GTTCGCGCTG CTGCTGCAGC TGGCGCCCGC CAGGCTGCTG 
CCGAGCTGCG CGCTCTTCCA GGACCTGCTC CGCTACGGGA AGACCAAGCA GTCCGGCTCG 
CGGCGCCCCG CCGTCTGCAG GGCCTTCGAT GTCCCCAAGA GGTACTTTTC TCACTTCTAC 
GTCATCTCAG TTGTGTGGAA TGGCTCCCTG CTCTGGCTAC TTTCTCAGTC GTTGTTCCTG 
GGAGCACCTT TTCCAAACTG GCTTAGTGCT CTGCTCAGAA CTCTTGGGGC CACACAGTTC 
CAAGCCCTGG AGATGGAGTC CAAGGCTTCT CGGATGCCAG CGGCTGAGCT GGCTCTGTCT 
GCCTTCTTGG TCTTGGTGTT CCTCTGGGTC CACAGCCTTC GGAGACTCTT TGAGTGCTTC 
TACGTCAGTG TCTTCTCTAA TGCAGCCATT CACGTTGTGC AGTACTGTTT CGGGCTTGTC 
TACTATGTGC TTGTTGGCCT GACTGTACTG AGCCAAGTGC CCATGGATGA TAAGAATGTG 
TATGTTCTGG GGAAGAATCT ACTGATACAA GCCCGGTGGT TCCACATCCT GGGCATGGTG 
ATGTTCTTCT GGTCATCCGC CCATCAGTAT AAATGCCACG TCATCCTCAG CAACCTCAGG 
AGAAACAAAA AAGGTGTGGT CATCCACTGC CAGCACCGGA TCCCCTTTGG AGACTGGTTC 
GAGTACGTGT CTTCAGCTAA CTACCTAGCA GAGCTGATGA TCTACATCTC CATGGCTGTC 
ACCTTTGGGC TCCACAACTT AACCTGGTGG CTGGTGGTGA CGTATGTCTT CTCCAGCCAA 
GCCTTGTCCG CATTCTTCAA CCACAAGTTC TACAGAAGCA CATTTGTCTC CTACCCAAAG 
CATAGGAAAG CGTTCCTCCC ATTTTTGTTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Srd5a3 ( XM_006535162.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006535225.1 Mus musculus steroid 5 alpha-reductase 3 (Srd5a3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu35629
Clone ID Related Accession (Same CDS sequence)	XM_006535162.1
Accession Version	XM_006535162.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 792bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	polyprenol reductase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCCG CTGAGAATAT GTACTTTTCT CACTTCTACG TCATCTCAGT TGTGTGGAAT 
GGCTCCCTGC TCTGGCTACT TTCTCAGTCG TTGTTCCTGG GAGCACCTTT TCCAAACTGG 
CTTAGTGCTC TGCTCAGAAC TCTTGGGGCC ACACAGTTCC AAGCCCTGGA GATGGAGTCC 
AAGGCTTCTC GGATGCCAGC GGCTGAGCTG GCTCTGTCTG CCTTCTTGGT CTTGGTGTTC 
CTCTGGGTCC ACAGCCTTCG GAGACTCTTT GAGTGCTTCT ACGTCAGTGT CTTCTCTAAT 
GCAGCCATTC ACGTTGTGCA GTACTGTTTC GGGCTTGTCT ACTATGTGCT TGTTGGCCTG 
ACTGTACTGA GCCAAGTGCC CATGGATGAT AAGAATGTGT ATGTTCTGGG GAAGAATCTA 
CTGATACAAG CCCGGTGGTT CCACATCCTG GGCATGGTGA TGTTCTTCTG GTCATCCGCC 
CATCAGTATA AATGCCACGT CATCCTCAGC AACCTCAGGA GAAACAAAAA AGGTGTGGTC 
ATCCACTGCC AGCACCGGAT CCCCTTTGGA GACTGGTTCG AGTACGTGTC TTCAGCTAAC 
TACCTAGCAG AGCTGATGAT CTACATCTCC ATGGCTGTCA CCTTTGGGCT CCACAACTTA 
ACCTGGTGGC TGGTGGTGAC GTATGTCTTC TCCAGCCAAG CCTTGTCCGC ATTCTTCAAC 
CACAAGTTCT ACAGAAGCAC ATTTGTCTCC TACCCAAAGC ATAGGAAAGC GTTCCTCCCA 
TTTTTGTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006535225.1
CDS	188..979
Translation	MDSAENMYFSHFYVISVVWNGSLLWLLSQSLFLGAPFPNWLSALLRTLGATQFQALEMESKASRMPAAELALSAFLVLVFLWVHSLRRLFECFYVSVFSNAAIHVVQYCFGLVYYVLVGLTVLSQVPMDDKNVYVLGKNLLIQARWFHILGMVMFFWSSAHQYKCHVILSNLRRNKKGVVIHCQHRIPFGDWFEYVSSANYLAELMIYISMAVTFGLHNLTWWLVVTYVFSSQALSAFFNHKFYRSTFVSYPKHRKAFLPFLF

Target ORF information:

RefSeq Version	XM_006535162.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus steroid 5 alpha-reductase 3 (Srd5a3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006535162.1

ORF Insert Sequence:

ATGGACTCCG CTGAGAATAT GTACTTTTCT CACTTCTACG TCATCTCAGT TGTGTGGAAT 
GGCTCCCTGC TCTGGCTACT TTCTCAGTCG TTGTTCCTGG GAGCACCTTT TCCAAACTGG 
CTTAGTGCTC TGCTCAGAAC TCTTGGGGCC ACACAGTTCC AAGCCCTGGA GATGGAGTCC 
AAGGCTTCTC GGATGCCAGC GGCTGAGCTG GCTCTGTCTG CCTTCTTGGT CTTGGTGTTC 
CTCTGGGTCC ACAGCCTTCG GAGACTCTTT GAGTGCTTCT ACGTCAGTGT CTTCTCTAAT 
GCAGCCATTC ACGTTGTGCA GTACTGTTTC GGGCTTGTCT ACTATGTGCT TGTTGGCCTG 
ACTGTACTGA GCCAAGTGCC CATGGATGAT AAGAATGTGT ATGTTCTGGG GAAGAATCTA 
CTGATACAAG CCCGGTGGTT CCACATCCTG GGCATGGTGA TGTTCTTCTG GTCATCCGCC 
CATCAGTATA AATGCCACGT CATCCTCAGC AACCTCAGGA GAAACAAAAA AGGTGTGGTC 
ATCCACTGCC AGCACCGGAT CCCCTTTGGA GACTGGTTCG AGTACGTGTC TTCAGCTAAC 
TACCTAGCAG AGCTGATGAT CTACATCTCC ATGGCTGTCA CCTTTGGGCT CCACAACTTA 
ACCTGGTGGC TGGTGGTGAC GTATGTCTTC TCCAGCCAAG CCTTGTCCGC ATTCTTCAAC 
CACAAGTTCT ACAGAAGCAC ATTTGTCTCC TACCCAAAGC ATAGGAAAGC GTTCCTCCCA 
TTTTTGTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Systematic screening for skin, hair, and nail abnormalities in a large-scale knockout mouse program.
PloS one12(7)e0180682(2017)
Sundberg JP,Dadras SS,Silva KA,Kennedy VE,Garland G,Murray SA,Sundberg BA,Schofield PN,Pratt CH

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Peri-implantation lethality in mice carrying megabase-scale deletion on 5qc3.3 is caused by Exoc1 null mutation.
Scientific reports513632(2015 Sep)
Mizuno S,Takami K,Daitoku Y,Tanimoto Y,Dinh TT,Mizuno-Iijima S,Hasegawa Y,Takahashi S,Sugiyama F,Yagami K

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

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Srd5a3 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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