Icmt cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Icmt
Entrez Gene ID	57295
Full Name	isoprenylcysteine carboxyl methyltransferase
Synonyms	1700008E11Rik,C80758,Gm13095,HSTE14,PPMT,STE14,pcCMT
General protein information	Preferred Names isoprenylcysteine carboxyl methyltransferase Names protein-S-isoprenylcysteine O-methyltransferase prenylated protein carboxyl methyltransferase prenylcysteine carboxyl methyltransferase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4\|4 E2

mRNA and Protein(s)

mRNA	Protein	Name
NM_133788.2	NP_598549.1	protein-S-isoprenylcysteine O-methyltransferase

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Icmt	NP_579844.2
Xenopus tropicalis (tropical clawed frog)	icmt	XP_002933887.1
Magnaporthe oryzae (rice blast fungus)	MGG_05331	XP_003712895.1
Homo sapiens (human)	ICMT	NP_036537.1
Gallus gallus (chicken)	ICMT	NP_001006298.1
Danio rerio (zebrafish)	icmt	NP_001017618.1
Caenorhabditis elegans (roundworm)	M01E11.1	NP_491635.1
Kluyveromyces lactis	KLLA0A02167g	XP_451096.1
Mus musculus (house mouse)	Icmt	NP_598549.1
Caenorhabditis elegans (roundworm)	F21F3.3	NP_491473.1
Eremothecium gossypii	AGOS_AAR122C	NP_982664.1
Arabidopsis thaliana (thale cress)	ATSTE14B	NP_568191.1
Oryza sativa (rice)	Os04g0602900	NP_001053777.1
Pan troglodytes (chimpanzee)	ICMT	XP_525166.2
Canis lupus familiaris (dog)	ICMT	XP_546748.1
Drosophila melanogaster (fruit fly)	ste14	NP_001261793.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001310	XP_321837.3
Saccharomyces cerevisiae (baker's yeast)	STE14	NP_010698.1
Schizosaccharomyces pombe (fission yeast)	mam4	NP_593263.1
Neurospora crassa	NCU00034	XP_957021.1
Arabidopsis thaliana (thale cress)	STE14A	NP_197723.1

Gene Ontology
Bibliography

Related articles in PubMed

Deficiency of Isoprenylcysteine Carboxyl Methyltransferase (ICMT) Leads to Progressive Loss of Photoreceptor Function.
Christiansen JR, Pendse ND, Kolandaivelu S, Bergo MO, Young SG, Ramamurthy V
The Journal of neuroscience : the official journal of the Society for Neuroscience36(18)5107-14(2016 05)

Inhibition of isoprenylcysteine carboxylmethyltransferase augments BCR-ABL1 tyrosine kinase inhibition-induced apoptosis in chronic myeloid leukemia.
Sun WT, Xiang W, Ng BL, Asari K, Bunte RM, Casey PJ, Wang M, Chuah C
Experimental hematology44(3)189-93.e2(2016 Mar)

Isoprenylcysteine carboxylmethyltransferase deficiency exacerbates KRAS-driven pancreatic neoplasia via Notch suppression.
Court H, Amoyel M, Hackman M, Lee KE, Xu R, Miller G, Bar-Sagi D, Bach EA, Berg? MO, Philips MR
The Journal of clinical investigation123(11)4681-94(2013 Nov)

Targeting isoprenylcysteine methylation ameliorates disease in a mouse model of progeria.
Ibrahim MX, Sayin VI, Akula MK, Liu M, Fong LG, Young SG, Bergo MO
Science (New York, N.Y.)340(6138)1330-3(2013 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

simultaneous inhibition of BCR-ABL1 and Icmt may represent a potential therapeutic strategy for CML.
Title: Inhibition of isoprenylcysteine carboxylmethyltransferase augments BCR-ABL1 tyrosine kinase inhibition-induced apoptosis in chronic myeloid leukemia.

ICMT deficiency precisely phenocopied Notch1 deficiency in the Pdx1-Cre;LSL-KrasG12D model by exacerbating pancreatic intraepithelial neoplasias, promoting facial papillomas, and derepressing Wnt signaling.
Title: Isoprenylcysteine carboxylmethyltransferase deficiency exacerbates KRAS-driven pancreatic neoplasia via Notch suppression.

inactivating Icmt reduced splenomegaly, the number of immature myeloid cells in peripheral blood, tissue infiltration by myeloid cells, reduced lung tumor development and myeloproliferation phenotypes in a mouse model of K-RAS-induced cancer.
Title: Inactivating Icmt ameliorates K-RAS-induced myeloproliferative disease.

findings suggest that inhibition of isoprenylcysteine-O-carboxyl methyltransferase (ICMT) causes endothelial cell apoptosis by attenuation of Ras GTPase methylation and activation and its downstream antiapoptotic signaling pathway.
Title: Isoprenylcysteine carboxyl methyltransferase activity modulates endothelial cell apoptosis.

The following Icmt gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Icmt cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu14097	NM_133788.2 Latest version!	Mus musculus isoprenylcysteine carboxyl methyltransferase (Icmt), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Icmt ( NM_133788.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_598549.1 Mus musculus isoprenylcysteine carboxyl methyltransferase (Icmt), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu14097
Clone ID Related Accession (Same CDS sequence)	NM_133788.2
Accession Version	NM_133788.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 855bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Mus musculus(house mouse)
Product	protein-S-isoprenylcysteine O-methyltransferase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL611985.22, AF209926.1 and BG404528.1. On Feb 24, 2011 this sequence version replaced NM_133788.1. Transcript Variant: This variant (1) encodes a functional protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF209926.1, BC110695.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGGCT GCGCGGCGCG GGTTCCGCCG GGCTCCGAGG CGCGCCTCAG CCTCGCTACA 
TTCCTCCTGG GCGCCTCGGT GCTCGCTCTG CCGCTGCTCA CGCGCGCCGG CCTGCAGGGC 
CGCACCGGGC TGGCGCTCTA CGTGGCCGGA CTCAACGCGC TGCTGCTGCT ACTCTACCGG 
CCGCCGCGCT ACCAGATAGC CATCAGAGCT TGTTTCCTTG GCTTTGTGTT TGGCTGTGGT 
GTGCTGCTAA GTTTCAGCCA GTCCTCTTGG AATCACTTTG GCTGGTACGT GTGCTCGCTG 
TCTCTGTTCC ACTATTCTGA GTACTTAGTG ACAGCTGTCA ATAACCCCAA AAGTCTGTCC 
CTGGACTCCT TCCTCCTGAA TCACAGCCTG GAGTACACGG TAGCTGCCCT GTCTTCTTGG 
ATAGAGTTCA CGCTCGAGAA CATCTTCTGG CCAGAACTGA AGCAGATTAC CTGGCTGAGT 
GCCACCGGGC TCCTGATGGT GGTCTTCGGA GAATGCCTGA GGAAAGCTGC CATGTTTACG 
GCTGGCTCCA ACTTCAACCA TGTGGTGCAG AGTGAAAAGT CAGACACCCA CACTCTGGTG 
ACAAGTGGGG TGTATGCTTG GTGCCGGCAC CCCTCCTATG TGGGCTGGTT TTACTGGAGT 
ATCGGAACCC AGGTGATGCT GTGTAATCCC ATCTGTGGCG TGGTCTATGC CCTGACGGTG 
TGGCGCTTCT TCCGAGATCG CACAGAAGAG GAGGAGATCT CACTGATTCA CTTTTTTGGG 
GAAGAGTACT TGGATTATAA AAAGAGGGTG CCCACAGGCC TGCCTTTCAT AAAGGGGGTC 
AAGGTGGAGC TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_598549.1
CDS	161..1015
Translation	MAGCAARVPPGSEARLSLATFLLGASVLALPLLTRAGLQGRTGLALYVAGLNALLLLLYRPPRYQIAIRACFLGFVFGCGVLLSFSQSSWNHFGWYVCSLSLFHYSEYLVTAVNNPKSLSLDSFLLNHSLEYTVAALSSWIEFTLENIFWPELKQITWLSATGLLMVVFGECLRKAAMFTAGSNFNHVVQSEKSDTHTLVTSGVYAWCRHPSYVGWFYWSIGTQVMLCNPICGVVYALTVWRFFRDRTEEEEISLIHFFGEEYLDYKKRVPTGLPFIKGVKVEL

Target ORF information:

RefSeq Version	NM_133788.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus isoprenylcysteine carboxyl methyltransferase (Icmt), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133788.2

ORF Insert Sequence:

ATGGCGGGCT GCGCGGCGCG GGTTCCGCCG GGCTCCGAGG CGCGCCTCAG CCTCGCTACA 
TTCCTCCTGG GCGCCTCGGT GCTCGCTCTG CCGCTGCTCA CGCGCGCCGG CCTGCAGGGC 
CGCACCGGGC TGGCGCTCTA CGTGGCCGGA CTCAACGCGC TGCTGCTGCT ACTCTACCGG 
CCGCCGCGCT ACCAGATAGC CATCAGAGCT TGTTTCCTTG GCTTTGTGTT TGGCTGTGGT 
GTGCTGCTAA GTTTCAGCCA GTCCTCTTGG AATCACTTTG GCTGGTACGT GTGCTCGCTG 
TCTCTGTTCC ACTATTCTGA GTACTTAGTG ACAGCTGTCA ATAACCCCAA AAGTCTGTCC 
CTGGACTCCT TCCTCCTGAA TCACAGCCTG GAGTACACGG TAGCTGCCCT GTCTTCTTGG 
ATAGAGTTCA CGCTCGAGAA CATCTTCTGG CCAGAACTGA AGCAGATTAC CTGGCTGAGT 
GCCACCGGGC TCCTGATGGT GGTCTTCGGA GAATGCCTGA GGAAAGCTGC CATGTTTACG 
GCTGGCTCCA ACTTCAACCA TGTGGTGCAG AGTGAAAAGT CAGACACCCA CACTCTGGTG 
ACAAGTGGGG TGTATGCTTG GTGCCGGCAC CCCTCCTATG TGGGCTGGTT TTACTGGAGT 
ATCGGAACCC AGGTGATGCT GTGTAATCCC ATCTGTGGCG TGGTCTATGC CCTGACGGTG 
TGGCGCTTCT TCCGAGATCG CACAGAAGAG GAGGAGATCT CACTGATTCA CTTTTTTGGG 
GAAGAGTACT TGGATTATAA AAAGAGGGTG CCCACAGGCC TGCCTTTCAT AAAGGGGGTC 
AAGGTGGAGC TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Deficiency of Isoprenylcysteine Carboxyl Methyltransferase (ICMT) Leads to Progressive Loss of Photoreceptor Function.
The Journal of neuroscience : the official journal of the Society for Neuroscience36(18)5107-14(2016 05)
Christiansen JR,Pendse ND,Kolandaivelu S,Bergo MO,Young SG,Ramamurthy V

Inhibition of isoprenylcysteine carboxylmethyltransferase augments BCR-ABL1 tyrosine kinase inhibition-induced apoptosis in chronic myeloid leukemia.
Experimental hematology44(3)189-93.e2(2016 Mar)
Sun WT,Xiang W,Ng BL,Asari K,Bunte RM,Casey PJ,Wang M,Chuah C

Isoprenylcysteine carboxylmethyltransferase deficiency exacerbates KRAS-driven pancreatic neoplasia via Notch suppression.
The Journal of clinical investigation123(11)4681-94(2013 Nov)
Court H,Amoyel M,Hackman M,Lee KE,Xu R,Miller G,Bar-Sagi D,Bach EA,Berg? MO,Philips MR

Targeting isoprenylcysteine methylation ameliorates disease in a mouse model of progeria.
Science (New York, N.Y.)340(6138)1330-3(2013 Jun)
Ibrahim MX,Sayin VI,Akula MK,Liu M,Fong LG,Young SG,Bergo MO

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Icmt cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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