Brd4 gene cDNA ORF clone, Mus musculus(house mouse)

mRNA	Protein	Name
XM_006524683.2	XP_006524746.1	bromodomain-containing protein 4 isoform X1
XM_006524683.3	XP_006524746.1	bromodomain-containing protein 4 isoform X1
XM_030249927.1	XP_030105787.1	bromodomain-containing protein 4 isoform X2
XM_030249928.1	XP_030105788.1	bromodomain-containing protein 4 isoform X4
XM_006524684.2	XP_006524747.1	bromodomain-containing protein 4 isoform X1
XM_006524690.2	XP_006524753.1	bromodomain-containing protein 4 isoform X3
XM_011246543.2	XP_011244845.1	bromodomain-containing protein 4 isoform X5
XM_030249922.1	XP_030105782.1	bromodomain-containing protein 4 isoform X1
XM_006524686.2	XP_006524749.1	bromodomain-containing protein 4 isoform X1
XM_030249925.1	XP_030105785.1	bromodomain-containing protein 4 isoform X2
XM_030249929.1	XP_030105789.1	bromodomain-containing protein 4 isoform X5
XM_011246543.3	XP_011244845.1	bromodomain-containing protein 4 isoform X5
NM_198094.2	NP_932762.2	bromodomain-containing protein 4 isoform 2
XM_006524687.2	XP_006524750.1	bromodomain-containing protein 4 isoform X1
XM_006524689.2	XP_006524752.1	bromodomain-containing protein 4 isoform X2
XM_006524691.1	XP_006524754.1	bromodomain-containing protein 4 isoform X4
XM_006524693.2	XP_006524756.1	bromodomain-containing protein 4 isoform X6
XM_006524690.3	XP_006524753.1	bromodomain-containing protein 4 isoform X3
XM_006524691.2	XP_006524754.1	bromodomain-containing protein 4 isoform X4
XM_006524693.3	XP_006524756.1	bromodomain-containing protein 4 isoform X6
XM_030249932.1	XP_030105792.1	bromodomain-containing protein 4 isoform X6
NM_020508.4	NP_065254.3	bromodomain-containing protein 4 isoform 1
XM_006524688.1	XP_006524751.1	bromodomain-containing protein 4 isoform X2
XM_006524695.3	XP_006524758.1	bromodomain-containing protein 4 isoform X7
XM_006524685.2	XP_006524748.1	bromodomain-containing protein 4 isoform X1
XM_030249923.1	XP_030105783.1	bromodomain-containing protein 4 isoform X1
XM_006524688.2	XP_006524751.1	bromodomain-containing protein 4 isoform X2
XM_030249926.1	XP_030105786.1	bromodomain-containing protein 4 isoform X2
XM_030249930.1	XP_030105790.1	bromodomain-containing protein 4 isoform X6
XM_006524695.4	XP_006524758.1	bromodomain-containing protein 4 isoform X7
NM_001286630.1	NP_001273559.1	bromodomain-containing protein 4 isoform 3
XM_006524685.1	XP_006524748.1	bromodomain-containing protein 4 isoform X1
XM_006524686.1	XP_006524749.1	bromodomain-containing protein 4 isoform X1
XM_017317588.1	XP_017173077.1	bromodomain-containing protein 4 isoform X5
XM_006524684.3	XP_006524747.1	bromodomain-containing protein 4 isoform X1
XM_006524689.3	XP_006524752.1	bromodomain-containing protein 4 isoform X2
XM_030249924.1	XP_030105784.1	bromodomain-containing protein 4 isoform X2
XM_017317588.2	XP_017173077.1	bromodomain-containing protein 4 isoform X5
XM_030249931.1	XP_030105791.1	bromodomain-containing protein 4 isoform X6

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu15435	NM_198094.2 Latest version!	Mus musculus bromodomain containing 4 (Brd4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OMu53867	XM_006524695.4 Latest version!	Mus musculus bromodomain containing 4 (Brd4), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu17143	NM_001286630.1 Latest version!	Mus musculus bromodomain containing 4 (Brd4), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OMu16383	XM_030249927.1 Latest version!	Mus musculus bromodomain containing 4 (Brd4), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu16383	XM_030249926.1 Latest version!	Mus musculus bromodomain containing 4 (Brd4), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu16383	XM_006524689.3 Latest version!	Mus musculus bromodomain containing 4 (Brd4), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu16383	XM_006524688.2 Latest version!	Mus musculus bromodomain containing 4 (Brd4), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu16383	XM_030249925.1 Latest version!	Mus musculus bromodomain containing 4 (Brd4), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu16383	XM_030249924.1 Latest version!	Mus musculus bromodomain containing 4 (Brd4), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu17143	XM_006524687.2 Latest version!	Mus musculus bromodomain containing 4 (Brd4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OMu17143	XM_006524686.2 Latest version!	Mus musculus bromodomain containing 4 (Brd4), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu17143	XM_030249923.1 Latest version!	Mus musculus bromodomain containing 4 (Brd4), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu17143	XM_006524685.2 Latest version!	Mus musculus bromodomain containing 4 (Brd4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu17143	XM_006524684.3 Latest version!	Mus musculus bromodomain containing 4 (Brd4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu17143	XM_030249922.1 Latest version!	Mus musculus bromodomain containing 4 (Brd4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu17143	XM_006524683.3 Latest version!	Mus musculus bromodomain containing 4 (Brd4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu53863	XM_006524690.3 Latest version!	Mus musculus bromodomain containing 4 (Brd4), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu53864	XM_006524691.2 Latest version!	Mus musculus bromodomain containing 4 (Brd4), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu53864	XM_030249928.1 Latest version!	Mus musculus bromodomain containing 4 (Brd4), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu53865	XM_017317588.2 Latest version!	Mus musculus bromodomain containing 4 (Brd4), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu53865	XM_011246543.3 Latest version!	Mus musculus bromodomain containing 4 (Brd4), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu53865	XM_030249929.1 Latest version!	Mus musculus bromodomain containing 4 (Brd4), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu53866	XM_030249930.1 Latest version!	Mus musculus bromodomain containing 4 (Brd4), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu53866	XM_030249932.1 Latest version!	Mus musculus bromodomain containing 4 (Brd4), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu53866	XM_030249931.1 Latest version!	Mus musculus bromodomain containing 4 (Brd4), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu53866	XM_006524693.3 Latest version!	Mus musculus bromodomain containing 4 (Brd4), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu16383	NM_020508.4 Latest version!	Mus musculus bromodomain containing 4 (Brd4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
View Old Accession Versions >>
OMu17143	XM_006524683.2	Mus musculus bromodomain containing 4 (Brd4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OMu53865	XM_011246543.2	Mus musculus bromodomain containing 4 (Brd4), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OMu53863	XM_006524690.2	Mus musculus bromodomain containing 4 (Brd4), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OMu53864	XM_006524691.1	Mus musculus bromodomain containing 4 (Brd4), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OMu53866	XM_006524693.2	Mus musculus bromodomain containing 4 (Brd4), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OMu16383	XM_006524688.1	Mus musculus bromodomain containing 4 (Brd4), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OMu53867	XM_006524695.3	Mus musculus bromodomain containing 4 (Brd4), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu17143	XM_006524685.1	Mus musculus bromodomain containing 4 (Brd4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OMu17143	XM_006524686.1	Mus musculus bromodomain containing 4 (Brd4), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OMu17143	XM_006524684.2	Mus musculus bromodomain containing 4 (Brd4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OMu16383	XM_006524689.2	Mus musculus bromodomain containing 4 (Brd4), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OMu53865	XM_017317588.1	Mus musculus bromodomain containing 4 (Brd4), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
Hide Old Accession Versions >>

Brd4 ( NM_198094.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_932762.2 Mus musculus bromodomain containing 4 (Brd4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu15435
Clone ID Related Accession (Same CDS sequence)	NM_198094.2
Accession Version	NM_198094.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2172bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	bromodomain-containing protein 4 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY779550.1, AF461396.1 and BC138834.1. On Apr 2, 2009 this sequence version replaced NM_198094.1. Transcript Variant: This variant (2) has two alternate splice sites, one in the 5' UTR and one in the 5' coding region, and also lacks several exons but includes an alternate 3' terminal exon, compared to variant 3. The resulting isoform (2) lacks an internal aa, and has a shorter and distinct C-terminus, compared to isoform 3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC138834.1, AF461396.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGCC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGACAGACA 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCCGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAAAAA 
ATCAATGAAC TGCCTACAGA AGAAACTGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGAGGGAGGA AAGAAACAGG GGCAGCAAAG CCTGGTGTAT CCACGGTACC AAACACAACT 
CAAGCATCAA CTTCTCCGCA GACCCAGACG CCTCAGCAGA ACCCTCCTCC ACCTGTGCAG 
GCCACAACTC ACCCCTTTCC TGCTGTCACC CCAGACCTCA TTGCCCAGCC TCCTGTCATG 
ACAATGGTGC CCCCTCAGCC ACTTCAGACT CCTTCACCGG TACCCCCCCA GCCACCACCC 
CCACCTGCTC CAGTTCCACA GCCTGTGCAG AGTCACCCGC CCATCATTGC GACCACCCCC 
CAGCCTGTGA AGACAAAGAA AGGGGTGAAG AGGAAAGCAG ATACCACCAC CCCTACCACC 
ATCGACCCCA TTCATGAGCC ACCCTCACTG GCCCCAGAGC CCAAGACCGC CAAGCTGGGT 
CCTCGGCGGG AGAGCAGCAG ACCTGTGAAG CCTCCAAAGA AGGATGTACC GGACTCACAG 
CAGCACCCAG GGCCAGAGAA GAGCAGCAAG ATCTCTGAGC AGCTAAAGTG CTGCAGTGGC 
ATCCTCAAGG AGATGTTTGC CAAGAAACAT GCTGCCTATG CCTGGCCTTT CTACAAGCCT 
GTGGATGTGG AGGCACTGGG TCTGCACGAC TACTGTGACA TCATCAAACA TCCCATGGAC 
ATGAGCACAA TCAAGTCTAA ACTAGAGTCC CGAGAGTACA GAGATGCCCA GGAATTTGGT 
GCTGATGTCC GATTGATGTT CTCCAACTGC TACAAGTACA ACCCCCCTGA CCATGAAGTG 
GTAGCCATGG CTCGAAAACT CCAGGATGTG TTTGAAATGC GCTTTGCCAA GATGCCTGAT 
GAGCCTGAAG AGCCAGTTGT TACAGTGTCC TCTCCTGCAG TGCCACCCCC TACAAAGGTG 
GTAGCCCCAC CCTCATCTAG TGACAGCAGC AGCGACAGTT CTTCCGACAG CGACAGTTCC 
ACTGACGACT CTGAGGAAGA GCGAGCCCAG CGGCTGGCTG AACTCCAGGA ACAGCTCAAG 
GCCGTGCATG AGCAGCTTGC AGCCCTCTCA CAGCCCCAGC AGAACAAACC AAAGAAAAAG 
GAGAAGGACA AGAAGGAAAA GAAAAAGGAA AAGCACAAAA AGAAAGAAGA AGTGGAGGAA 
AATAAAAAAA GCAAAACCAA GGAACTTCCT CCCAAAAAGA CAAAGAAAAA TAACAGCAGC 
AACAGCAATG TGAGCAAGAA GGAACCAGTA CCCACGAAGA CCAAGCCGCC TCCCACATAT 
GAATCAGAAG AGGAGGATAA GTGTAAGCCC ATGTCTTATG AGGAGAAGCG GCAGCTAAGT 
CTAGATATCA ACAAACTTCC TGGTGAGAAG CTAGGCCGTG TAGTACACAT AATTCAGTCA 
AGGGAACCAT CACTTAAAAA CTCCAACCCC GATGAGATTG AGATTGACTT TGAGACCCTG 
AAGCCATCTA CACTACGAGA GTTGGAGCGA TATGTCACCT CCTGTTTGCG GAAGAAAAGG 
AAACCTCAAG CTGAAAAAGT TGACGTGATT GCTGGTTCTT CCAAGATGAA GGGATTCTCA 
TCCTCTGAGT CGGAGAGCAC CAGCGAATCC AGCTCCTCTG ACAGTGAAGA CTCTGAAACA 
GGTCCTGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_932762.2
CDS	256..2427
Translation	MSTESGPGTRLRNLPVMGDGLETSQMSTTQAQAQPQPANAASTNPPPPETSNPNKPKRQTNQLQYLLRVVLKTLWKHQFAWPFQQPVDAVKLNLPDYYKIIKTPMDMGTIKKRLENNYYWNAQECIQDFNTMFTNCYIYNKPGDDIVLMAEALEKLFLQKINELPTEETEIMIVQAKGRGRGRKETGAAKPGVSTVPNTTQASTSPQTQTPQQNPPPPVQATTHPFPAVTPDLIAQPPVMTMVPPQPLQTPSPVPPQPPPPPAPVPQPVQSHPPIIATTPQPVKTKKGVKRKADTTTPTTIDPIHEPPSLAPEPKTAKLGPRRESSRPVKPPKKDVPDSQQHPGPEKSSKISEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYCDIIKHPMDMSTIKSKLESREYRDAQEFGADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPEEPVVTVSSPAVPPPTKVVAPPSSSDSSSDSSSDSDSSTDDSEEERAQRLAELQEQLKAVHEQLAALSQPQQNKPKKKEKDKKEKKKEKHKKKEEVEENKKSKTKELPPKKTKKNNSSNSNVSKKEPVPTKTKPPPTYESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVDVIAGSSKMKGFSSSESESTSESSSSDSEDSETGPA

Target ORF information:

RefSeq Version	NM_198094.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus bromodomain containing 4 (Brd4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_198094.2

ORF Insert Sequence:

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGCC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGACAGACA 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCCGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAAAAA 
ATCAATGAAC TGCCTACAGA AGAAACTGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGAGGGAGGA AAGAAACAGG GGCAGCAAAG CCTGGTGTAT CCACGGTACC AAACACAACT 
CAAGCATCAA CTTCTCCGCA GACCCAGACG CCTCAGCAGA ACCCTCCTCC ACCTGTGCAG 
GCCACAACTC ACCCCTTTCC TGCTGTCACC CCAGACCTCA TTGCCCAGCC TCCTGTCATG 
ACAATGGTGC CCCCTCAGCC ACTTCAGACT CCTTCACCGG TACCCCCCCA GCCACCACCC 
CCACCTGCTC CAGTTCCACA GCCTGTGCAG AGTCACCCGC CCATCATTGC GACCACCCCC 
CAGCCTGTGA AGACAAAGAA AGGGGTGAAG AGGAAAGCAG ATACCACCAC CCCTACCACC 
ATCGACCCCA TTCATGAGCC ACCCTCACTG GCCCCAGAGC CCAAGACCGC CAAGCTGGGT 
CCTCGGCGGG AGAGCAGCAG ACCTGTGAAG CCTCCAAAGA AGGATGTACC GGACTCACAG 
CAGCACCCAG GGCCAGAGAA GAGCAGCAAG ATCTCTGAGC AGCTAAAGTG CTGCAGTGGC 
ATCCTCAAGG AGATGTTTGC CAAGAAACAT GCTGCCTATG CCTGGCCTTT CTACAAGCCT 
GTGGATGTGG AGGCACTGGG TCTGCACGAC TACTGTGACA TCATCAAACA TCCCATGGAC 
ATGAGCACAA TCAAGTCTAA ACTAGAGTCC CGAGAGTACA GAGATGCCCA GGAATTTGGT 
GCTGATGTCC GATTGATGTT CTCCAACTGC TACAAGTACA ACCCCCCTGA CCATGAAGTG 
GTAGCCATGG CTCGAAAACT CCAGGATGTG TTTGAAATGC GCTTTGCCAA GATGCCTGAT 
GAGCCTGAAG AGCCAGTTGT TACAGTGTCC TCTCCTGCAG TGCCACCCCC TACAAAGGTG 
GTAGCCCCAC CCTCATCTAG TGACAGCAGC AGCGACAGTT CTTCCGACAG CGACAGTTCC 
ACTGACGACT CTGAGGAAGA GCGAGCCCAG CGGCTGGCTG AACTCCAGGA ACAGCTCAAG 
GCCGTGCATG AGCAGCTTGC AGCCCTCTCA CAGCCCCAGC AGAACAAACC AAAGAAAAAG 
GAGAAGGACA AGAAGGAAAA GAAAAAGGAA AAGCACAAAA AGAAAGAAGA AGTGGAGGAA 
AATAAAAAAA GCAAAACCAA GGAACTTCCT CCCAAAAAGA CAAAGAAAAA TAACAGCAGC 
AACAGCAATG TGAGCAAGAA GGAACCAGTA CCCACGAAGA CCAAGCCGCC TCCCACATAT 
GAATCAGAAG AGGAGGATAA GTGTAAGCCC ATGTCTTATG AGGAGAAGCG GCAGCTAAGT 
CTAGATATCA ACAAACTTCC TGGTGAGAAG CTAGGCCGTG TAGTACACAT AATTCAGTCA 
AGGGAACCAT CACTTAAAAA CTCCAACCCC GATGAGATTG AGATTGACTT TGAGACCCTG 
AAGCCATCTA CACTACGAGA GTTGGAGCGA TATGTCACCT CCTGTTTGCG GAAGAAAAGG 
AAACCTCAAG CTGAAAAAGT TGACGTGATT GCTGGTTCTT CCAAGATGAA GGGATTCTCA 
TCCTCTGAGT CGGAGAGCAC CAGCGAATCC AGCTCCTCTG ACAGTGAAGA CTCTGAAACA 
GGTCCTGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brd4 ( XM_006524695.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524758.1 Mus musculus bromodomain containing 4 (Brd4), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OMu53867
Clone ID Related Accession (Same CDS sequence)	XM_006524695.3 , XM_006524695.4
Accession Version	XM_006524695.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	bromodomain-containing protein 4 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006524695.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGCC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGACAGACA 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCCGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAAAAA 
ATCAATGAAC TGCCTACAGA AGAAACTGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGAGGGAGGA AAGAAACAGG TAGGGCAGCA AAGCCTGGTG TATCCACGGT ACCAAACACA 
ACTCAAGCAT CAACTTCTCC GCAGACCCAG ACGCCTCAGC AGAACCCTCC TCCACCTGTG 
CAGGCCACAA CTCACCCCTT TCCTGCTGTC ACCCCAGACC TCATTGCCCA GCCTCCTGTC 
ATGACAATGG TGCCCCCTCA GCCACTTCAG ACTCCTTCAC CGGTACCCCC CCAGCCACCA 
CCCCCACCTG CTCCAGTTCC ACAGCCTGTG CAGAGTCACC CGCCCATCAT TGCGACCACC 
CCCCAGCCTG TGAAGACAAA GAAAGGGGTG AAGAGGAAAG CAGATACCAC CACCCCTACC 
ACCATCGACC CCATTCATGA GCCACCCTCA CTGGCCCCAG AGCCCAAGAC CGCCAAGCTG 
GGTCCTCGGC GGGAGAGCAG CAGACCTGTG AAGCCTCCAA AGAAGGATGT ACCGGACTCA 
CAGCAGCACC CAGGGCCAGA GAAGAGCAGC AAGATCTCTG AGCAGCTAAA GTGCTGCAGT 
GGCATCCTCA AGGAGATGTT TGCCAAGAAA CATGCTGCCT ATGCCTGGCC TTTCTACAAG 
CCTGTGGATG TGGAGGCACT GGGTCTGCAC GACTACTGTG ACATCATCAA ACATCCCATG 
GACATGAGCA CAATCAAGTC TAAACTAGAG TCCCGAGAGT ACAGAGATGC CCAGGAATTT 
GGTGCTGATG TCCGATTGAT GTTCTCCAAC TGCTACAAGT ACAACCCCCC TGACCATGAA 
GTGGTAGCCA TGGCTCGAAA ACTCCAGGAT GTGTTTGAAA TGCGCTTTGC CAAGATGCCT 
GATGAGCCTG AAGAGCCAGT TGTTACAGTG TCCTCTCCTG CAGTGCCACC CCCTACAAAG 
GTGGTAGCCC CACCCTCATC TAGTGACAGC AGCAGCGACA GTTCTTCCGA CAGCGACAGT 
TCCACTGACG ACTCTGAGGA AGAGCGAGCC CAGCGGCTGG CTGAACTCCA GGAACAGCTC 
AAGGCCGTGC ATGAGCAGCT TGCAGCCCTC TCACAGCCCC AGCAGAACAA ACCAAAGAAA 
AAGGAGAAGG ACAAGAAGGA AAAGAAAAAG GAAAAGCACA AAAAGAAAGA AGAAGTGGAG 
GAAAATAAAA AAAGCAAAAC CAAGGAACTT CCTCCCAAAA AGACAAAGAA AAATAACAGC 
AGCAACAGCA ATGTGAGCAA GAAGGAACCA GTACCCACGA AGACCAAGCC GCCTCCCACA 
TATGAATCAG AAGAGGAGGA TAAGTGTAAG CCCATGTCTT ATGAGGAGAA GCGGCAGCTA 
AGTCTAGATA TCAACAAACT TCCTGGTGAG AAGCTAGGCC GTGTAGTACA CATAATTCAG 
TCAAGGGAAC CATCACTTAA AAACTCCAAC CCCGATGAGA TTGAGATTGA CTTTGAGACC 
CTGAAGCCAT CTACACTACG AGAGTTGGAG CGATATGTCA CCTCCTGTTT GCGGAAGAAA 
AGGAAACCTC AAGCTGAAAA AGTTGACGTG ATTGCTGGTT CTTCCAAGAT GAAGGGATTC 
TCATCCTCTG AGTCGGAGAG CACCAGCGAA TCCAGCTCCT CTGACAGTGA AGACTCTGAA 
ACAGGTCCTG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524758.1
CDS	109..2283
Translation	MSTESGPGTRLRNLPVMGDGLETSQMSTTQAQAQPQPANAASTNPPPPETSNPNKPKRQTNQLQYLLRVVLKTLWKHQFAWPFQQPVDAVKLNLPDYYKIIKTPMDMGTIKKRLENNYYWNAQECIQDFNTMFTNCYIYNKPGDDIVLMAEALEKLFLQKINELPTEETEIMIVQAKGRGRGRKETGRAAKPGVSTVPNTTQASTSPQTQTPQQNPPPPVQATTHPFPAVTPDLIAQPPVMTMVPPQPLQTPSPVPPQPPPPPAPVPQPVQSHPPIIATTPQPVKTKKGVKRKADTTTPTTIDPIHEPPSLAPEPKTAKLGPRRESSRPVKPPKKDVPDSQQHPGPEKSSKISEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYCDIIKHPMDMSTIKSKLESREYRDAQEFGADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPEEPVVTVSSPAVPPPTKVVAPPSSSDSSSDSSSDSDSSTDDSEEERAQRLAELQEQLKAVHEQLAALSQPQQNKPKKKEKDKKEKKKEKHKKKEEVEENKKSKTKELPPKKTKKNNSSNSNVSKKEPVPTKTKPPPTYESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVDVIAGSSKMKGFSSSESESTSESSSSDSEDSETGPA

Target ORF information:

RefSeq Version	XM_006524695.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus bromodomain containing 4 (Brd4), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524695.3

ORF Insert Sequence:

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGCC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGACAGACA 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCCGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAAAAA 
ATCAATGAAC TGCCTACAGA AGAAACTGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGAGGGAGGA AAGAAACAGG TAGGGCAGCA AAGCCTGGTG TATCCACGGT ACCAAACACA 
ACTCAAGCAT CAACTTCTCC GCAGACCCAG ACGCCTCAGC AGAACCCTCC TCCACCTGTG 
CAGGCCACAA CTCACCCCTT TCCTGCTGTC ACCCCAGACC TCATTGCCCA GCCTCCTGTC 
ATGACAATGG TGCCCCCTCA GCCACTTCAG ACTCCTTCAC CGGTACCCCC CCAGCCACCA 
CCCCCACCTG CTCCAGTTCC ACAGCCTGTG CAGAGTCACC CGCCCATCAT TGCGACCACC 
CCCCAGCCTG TGAAGACAAA GAAAGGGGTG AAGAGGAAAG CAGATACCAC CACCCCTACC 
ACCATCGACC CCATTCATGA GCCACCCTCA CTGGCCCCAG AGCCCAAGAC CGCCAAGCTG 
GGTCCTCGGC GGGAGAGCAG CAGACCTGTG AAGCCTCCAA AGAAGGATGT ACCGGACTCA 
CAGCAGCACC CAGGGCCAGA GAAGAGCAGC AAGATCTCTG AGCAGCTAAA GTGCTGCAGT 
GGCATCCTCA AGGAGATGTT TGCCAAGAAA CATGCTGCCT ATGCCTGGCC TTTCTACAAG 
CCTGTGGATG TGGAGGCACT GGGTCTGCAC GACTACTGTG ACATCATCAA ACATCCCATG 
GACATGAGCA CAATCAAGTC TAAACTAGAG TCCCGAGAGT ACAGAGATGC CCAGGAATTT 
GGTGCTGATG TCCGATTGAT GTTCTCCAAC TGCTACAAGT ACAACCCCCC TGACCATGAA 
GTGGTAGCCA TGGCTCGAAA ACTCCAGGAT GTGTTTGAAA TGCGCTTTGC CAAGATGCCT 
GATGAGCCTG AAGAGCCAGT TGTTACAGTG TCCTCTCCTG CAGTGCCACC CCCTACAAAG 
GTGGTAGCCC CACCCTCATC TAGTGACAGC AGCAGCGACA GTTCTTCCGA CAGCGACAGT 
TCCACTGACG ACTCTGAGGA AGAGCGAGCC CAGCGGCTGG CTGAACTCCA GGAACAGCTC 
AAGGCCGTGC ATGAGCAGCT TGCAGCCCTC TCACAGCCCC AGCAGAACAA ACCAAAGAAA 
AAGGAGAAGG ACAAGAAGGA AAAGAAAAAG GAAAAGCACA AAAAGAAAGA AGAAGTGGAG 
GAAAATAAAA AAAGCAAAAC CAAGGAACTT CCTCCCAAAA AGACAAAGAA AAATAACAGC 
AGCAACAGCA ATGTGAGCAA GAAGGAACCA GTACCCACGA AGACCAAGCC GCCTCCCACA 
TATGAATCAG AAGAGGAGGA TAAGTGTAAG CCCATGTCTT ATGAGGAGAA GCGGCAGCTA 
AGTCTAGATA TCAACAAACT TCCTGGTGAG AAGCTAGGCC GTGTAGTACA CATAATTCAG 
TCAAGGGAAC CATCACTTAA AAACTCCAAC CCCGATGAGA TTGAGATTGA CTTTGAGACC 
CTGAAGCCAT CTACACTACG AGAGTTGGAG CGATATGTCA CCTCCTGTTT GCGGAAGAAA 
AGGAAACCTC AAGCTGAAAA AGTTGACGTG ATTGCTGGTT CTTCCAAGAT GAAGGGATTC 
TCATCCTCTG AGTCGGAGAG CACCAGCGAA TCCAGCTCCT CTGACAGTGA AGACTCTGAA 
ACAGGTCCTG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brd4 ( XM_006524695.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524758.1 Mus musculus bromodomain containing 4 (Brd4), transcript variant X23, mRNA.

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CloneID	OMu53867
Clone ID Related Accession (Same CDS sequence)	XM_006524695.3 , XM_006524695.4
Accession Version	XM_006524695.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	bromodomain-containing protein 4 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006524695.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGCC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGACAGACA 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCCGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAAAAA 
ATCAATGAAC TGCCTACAGA AGAAACTGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGAGGGAGGA AAGAAACAGG TAGGGCAGCA AAGCCTGGTG TATCCACGGT ACCAAACACA 
ACTCAAGCAT CAACTTCTCC GCAGACCCAG ACGCCTCAGC AGAACCCTCC TCCACCTGTG 
CAGGCCACAA CTCACCCCTT TCCTGCTGTC ACCCCAGACC TCATTGCCCA GCCTCCTGTC 
ATGACAATGG TGCCCCCTCA GCCACTTCAG ACTCCTTCAC CGGTACCCCC CCAGCCACCA 
CCCCCACCTG CTCCAGTTCC ACAGCCTGTG CAGAGTCACC CGCCCATCAT TGCGACCACC 
CCCCAGCCTG TGAAGACAAA GAAAGGGGTG AAGAGGAAAG CAGATACCAC CACCCCTACC 
ACCATCGACC CCATTCATGA GCCACCCTCA CTGGCCCCAG AGCCCAAGAC CGCCAAGCTG 
GGTCCTCGGC GGGAGAGCAG CAGACCTGTG AAGCCTCCAA AGAAGGATGT ACCGGACTCA 
CAGCAGCACC CAGGGCCAGA GAAGAGCAGC AAGATCTCTG AGCAGCTAAA GTGCTGCAGT 
GGCATCCTCA AGGAGATGTT TGCCAAGAAA CATGCTGCCT ATGCCTGGCC TTTCTACAAG 
CCTGTGGATG TGGAGGCACT GGGTCTGCAC GACTACTGTG ACATCATCAA ACATCCCATG 
GACATGAGCA CAATCAAGTC TAAACTAGAG TCCCGAGAGT ACAGAGATGC CCAGGAATTT 
GGTGCTGATG TCCGATTGAT GTTCTCCAAC TGCTACAAGT ACAACCCCCC TGACCATGAA 
GTGGTAGCCA TGGCTCGAAA ACTCCAGGAT GTGTTTGAAA TGCGCTTTGC CAAGATGCCT 
GATGAGCCTG AAGAGCCAGT TGTTACAGTG TCCTCTCCTG CAGTGCCACC CCCTACAAAG 
GTGGTAGCCC CACCCTCATC TAGTGACAGC AGCAGCGACA GTTCTTCCGA CAGCGACAGT 
TCCACTGACG ACTCTGAGGA AGAGCGAGCC CAGCGGCTGG CTGAACTCCA GGAACAGCTC 
AAGGCCGTGC ATGAGCAGCT TGCAGCCCTC TCACAGCCCC AGCAGAACAA ACCAAAGAAA 
AAGGAGAAGG ACAAGAAGGA AAAGAAAAAG GAAAAGCACA AAAAGAAAGA AGAAGTGGAG 
GAAAATAAAA AAAGCAAAAC CAAGGAACTT CCTCCCAAAA AGACAAAGAA AAATAACAGC 
AGCAACAGCA ATGTGAGCAA GAAGGAACCA GTACCCACGA AGACCAAGCC GCCTCCCACA 
TATGAATCAG AAGAGGAGGA TAAGTGTAAG CCCATGTCTT ATGAGGAGAA GCGGCAGCTA 
AGTCTAGATA TCAACAAACT TCCTGGTGAG AAGCTAGGCC GTGTAGTACA CATAATTCAG 
TCAAGGGAAC CATCACTTAA AAACTCCAAC CCCGATGAGA TTGAGATTGA CTTTGAGACC 
CTGAAGCCAT CTACACTACG AGAGTTGGAG CGATATGTCA CCTCCTGTTT GCGGAAGAAA 
AGGAAACCTC AAGCTGAAAA AGTTGACGTG ATTGCTGGTT CTTCCAAGAT GAAGGGATTC 
TCATCCTCTG AGTCGGAGAG CACCAGCGAA TCCAGCTCCT CTGACAGTGA AGACTCTGAA 
ACAGGTCCTG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524758.1
CDS	261..2435
Translation	MSTESGPGTRLRNLPVMGDGLETSQMSTTQAQAQPQPANAASTNPPPPETSNPNKPKRQTNQLQYLLRVVLKTLWKHQFAWPFQQPVDAVKLNLPDYYKIIKTPMDMGTIKKRLENNYYWNAQECIQDFNTMFTNCYIYNKPGDDIVLMAEALEKLFLQKINELPTEETEIMIVQAKGRGRGRKETGRAAKPGVSTVPNTTQASTSPQTQTPQQNPPPPVQATTHPFPAVTPDLIAQPPVMTMVPPQPLQTPSPVPPQPPPPPAPVPQPVQSHPPIIATTPQPVKTKKGVKRKADTTTPTTIDPIHEPPSLAPEPKTAKLGPRRESSRPVKPPKKDVPDSQQHPGPEKSSKISEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYCDIIKHPMDMSTIKSKLESREYRDAQEFGADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPEEPVVTVSSPAVPPPTKVVAPPSSSDSSSDSSSDSDSSTDDSEEERAQRLAELQEQLKAVHEQLAALSQPQQNKPKKKEKDKKEKKKEKHKKKEEVEENKKSKTKELPPKKTKKNNSSNSNVSKKEPVPTKTKPPPTYESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVDVIAGSSKMKGFSSSESESTSESSSSDSEDSETGPA

Target ORF information:

RefSeq Version	XM_006524695.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus bromodomain containing 4 (Brd4), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524695.4

ORF Insert Sequence:

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGCC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGACAGACA 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCCGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAAAAA 
ATCAATGAAC TGCCTACAGA AGAAACTGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGAGGGAGGA AAGAAACAGG TAGGGCAGCA AAGCCTGGTG TATCCACGGT ACCAAACACA 
ACTCAAGCAT CAACTTCTCC GCAGACCCAG ACGCCTCAGC AGAACCCTCC TCCACCTGTG 
CAGGCCACAA CTCACCCCTT TCCTGCTGTC ACCCCAGACC TCATTGCCCA GCCTCCTGTC 
ATGACAATGG TGCCCCCTCA GCCACTTCAG ACTCCTTCAC CGGTACCCCC CCAGCCACCA 
CCCCCACCTG CTCCAGTTCC ACAGCCTGTG CAGAGTCACC CGCCCATCAT TGCGACCACC 
CCCCAGCCTG TGAAGACAAA GAAAGGGGTG AAGAGGAAAG CAGATACCAC CACCCCTACC 
ACCATCGACC CCATTCATGA GCCACCCTCA CTGGCCCCAG AGCCCAAGAC CGCCAAGCTG 
GGTCCTCGGC GGGAGAGCAG CAGACCTGTG AAGCCTCCAA AGAAGGATGT ACCGGACTCA 
CAGCAGCACC CAGGGCCAGA GAAGAGCAGC AAGATCTCTG AGCAGCTAAA GTGCTGCAGT 
GGCATCCTCA AGGAGATGTT TGCCAAGAAA CATGCTGCCT ATGCCTGGCC TTTCTACAAG 
CCTGTGGATG TGGAGGCACT GGGTCTGCAC GACTACTGTG ACATCATCAA ACATCCCATG 
GACATGAGCA CAATCAAGTC TAAACTAGAG TCCCGAGAGT ACAGAGATGC CCAGGAATTT 
GGTGCTGATG TCCGATTGAT GTTCTCCAAC TGCTACAAGT ACAACCCCCC TGACCATGAA 
GTGGTAGCCA TGGCTCGAAA ACTCCAGGAT GTGTTTGAAA TGCGCTTTGC CAAGATGCCT 
GATGAGCCTG AAGAGCCAGT TGTTACAGTG TCCTCTCCTG CAGTGCCACC CCCTACAAAG 
GTGGTAGCCC CACCCTCATC TAGTGACAGC AGCAGCGACA GTTCTTCCGA CAGCGACAGT 
TCCACTGACG ACTCTGAGGA AGAGCGAGCC CAGCGGCTGG CTGAACTCCA GGAACAGCTC 
AAGGCCGTGC ATGAGCAGCT TGCAGCCCTC TCACAGCCCC AGCAGAACAA ACCAAAGAAA 
AAGGAGAAGG ACAAGAAGGA AAAGAAAAAG GAAAAGCACA AAAAGAAAGA AGAAGTGGAG 
GAAAATAAAA AAAGCAAAAC CAAGGAACTT CCTCCCAAAA AGACAAAGAA AAATAACAGC 
AGCAACAGCA ATGTGAGCAA GAAGGAACCA GTACCCACGA AGACCAAGCC GCCTCCCACA 
TATGAATCAG AAGAGGAGGA TAAGTGTAAG CCCATGTCTT ATGAGGAGAA GCGGCAGCTA 
AGTCTAGATA TCAACAAACT TCCTGGTGAG AAGCTAGGCC GTGTAGTACA CATAATTCAG 
TCAAGGGAAC CATCACTTAA AAACTCCAAC CCCGATGAGA TTGAGATTGA CTTTGAGACC 
CTGAAGCCAT CTACACTACG AGAGTTGGAG CGATATGTCA CCTCCTGTTT GCGGAAGAAA 
AGGAAACCTC AAGCTGAAAA AGTTGACGTG ATTGCTGGTT CTTCCAAGAT GAAGGGATTC 
TCATCCTCTG AGTCGGAGAG CACCAGCGAA TCCAGCTCCT CTGACAGTGA AGACTCTGAA 
ACAGGTCCTG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brd4 ( NM_001286630.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001273559.1 Mus musculus bromodomain containing 4 (Brd4), transcript variant 3, mRNA.

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CloneID	OMu17143
Clone ID Related Accession (Same CDS sequence)	XM_006524683.2 , XM_006524683.3 , XM_006524684.2 , XM_030249922.1 , XM_006524686.2 , XM_006524687.2 , XM_006524685.2 , XM_030249923.1 , NM_001286630.1 , XM_006524685.1 , XM_006524686.1 , XM_006524684.3
Accession Version	NM_001286630.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4206bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	bromodomain-containing protein 4 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY779550.1, AK147545.1 and CJ192204.1. Transcript Variant: This variant (3) encodes the longest isoform (3). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK147545.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGCC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGACAGACA 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCCGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAAAAA 
ATCAATGAAC TGCCTACAGA AGAAACTGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGAGGGAGGA AAGAAACAGG TAGGGCAGCA AAGCCTGGTG TATCCACGGT ACCAAACACA 
ACTCAAGCAT CAACTTCTCC GCAGACCCAG ACGCCTCAGC AGAACCCTCC TCCACCTGTG 
CAGGCCACAA CTCACCCCTT TCCTGCTGTC ACCCCAGACC TCATTGCCCA GCCTCCTGTC 
ATGACAATGG TGCCCCCTCA GCCACTTCAG ACTCCTTCAC CGGTACCCCC CCAGCCACCA 
CCCCCACCTG CTCCAGTTCC ACAGCCTGTG CAGAGTCACC CGCCCATCAT TGCGACCACC 
CCCCAGCCTG TGAAGACAAA GAAAGGGGTG AAGAGGAAAG CAGATACCAC CACCCCTACC 
ACCATCGACC CCATTCATGA GCCACCCTCA CTGGCCCCAG AGCCCAAGAC CGCCAAGCTG 
GGTCCTCGGC GGGAGAGCAG CAGACCTGTG AAGCCTCCAA AGAAGGATGT ACCGGACTCA 
CAGCAGCACC CAGGGCCAGA GAAGAGCAGC AAGATCTCTG AGCAGCTAAA GTGCTGCAGT 
GGCATCCTCA AGGAGATGTT TGCCAAGAAA CATGCTGCCT ATGCCTGGCC TTTCTACAAG 
CCTGTGGATG TGGAGGCACT GGGTCTGCAC GACTACTGTG ACATCATCAA ACATCCCATG 
GACATGAGCA CAATCAAGTC TAAACTAGAG TCCCGAGAGT ACAGAGATGC CCAGGAATTT 
GGTGCTGATG TCCGATTGAT GTTCTCCAAC TGCTACAAGT ACAACCCCCC TGACCATGAA 
GTGGTAGCCA TGGCTCGAAA ACTCCAGGAT GTGTTTGAAA TGCGCTTTGC CAAGATGCCT 
GATGAGCCTG AAGAGCCAGT TGTTACAGTG TCCTCTCCTG CAGTGCCACC CCCTACAAAG 
GTGGTAGCCC CACCCTCATC TAGTGACAGC AGCAGCGACA GTTCTTCCGA CAGCGACAGT 
TCCACTGACG ACTCTGAGGA AGAGCGAGCC CAGCGGCTGG CTGAACTCCA GGAACAGCTC 
AAGGCCGTGC ATGAGCAGCT TGCAGCCCTC TCACAGCCCC AGCAGAACAA ACCAAAGAAA 
AAGGAGAAGG ACAAGAAGGA AAAGAAAAAG GAAAAGCACA AAAAGAAAGA AGAAGTGGAG 
GAAAATAAAA AAAGCAAAAC CAAGGAACTT CCTCCCAAAA AGACAAAGAA AAATAACAGC 
AGCAACAGCA ATGTGAGCAA GAAGGAACCA GTACCCACGA AGACCAAGCC GCCTCCCACA 
TATGAATCAG AAGAGGAGGA TAAGTGTAAG CCCATGTCTT ATGAGGAGAA GCGGCAGCTA 
AGTCTAGATA TCAACAAACT TCCTGGTGAG AAGCTAGGCC GTGTAGTACA CATAATTCAG 
TCAAGGGAAC CATCACTTAA AAACTCCAAC CCCGATGAGA TTGAGATTGA CTTTGAGACC 
CTGAAGCCAT CTACACTACG AGAGTTGGAG CGATATGTCA CCTCCTGTTT GCGGAAGAAA 
AGGAAACCTC AAGCTGAAAA AGTTGACGTG ATTGCTGGTT CTTCCAAGAT GAAGGGATTC 
TCATCCTCTG AGTCGGAGAG CACCAGCGAA TCCAGCTCCT CTGACAGTGA AGACTCTGAA 
ACAGAGATGG CTCCCAAGTC AAAAAAGAAG GGGCACACTG GGAGGGACCA GAAAAAGCAT 
CATCACCATC ACCATCCACA GATGCAGCCA GCCCCAGCTC CTGTGCCCCA GCAGCCGCCC 
CCACCTCCAC AGCAGCCTCC TCCACCCCCA CCTCCGCAGC AGCAGCAGCA GCAACCTCCA 
CCCCCACCAC CTCCGCCCTC CATGCCACAG CAGACTGCCC CAGCGATGAA GTCCTCGCCC 
CCACCCTTCA TCACTGCCCA GGTCCCCGTC CTGGAACCAC AGCTGCCAGG CAGTGTCTTT 
GACCCTATTG GCCACTTCAC CCAGCCCATC TTGCACCTGC CGCAGCCGGA GCTGCCTCCT 
CACCTGCCCC AGCCACCTGA GCACAGCACT CCACCCCATC TCAACCAGCA TGCTGTGGTC 
TCTCCTCCAG CTTTGCACAA TGCGCTGCCC CAACAGCCAT CTCGGCCCAG TAACCGAGCT 
GCTGCTCTGC CCCCAAAGCC TACCCGACCC CCAGCTGTGT CCCCTGCCCT GGCCCAGCCC 
CCCCTGCTCC CACAACCACC AATGGCTCAG CCCCCCCAAG TGCTGCTGGA GGATGAAGAG 
CCACCTGCCC CACCCCTCAC CTCCATGCAG ATGCAGTTGT ACCTACAGCA GCTGCAGAAG 
GTGCAGCCTC CCACACCACT ACTCCCTTCC GTGAAGGTGC AGTCCCAACC CCCACCGCCT 
TTGCCGCCTC CACCCCACCC TTCTGTGCAG CAGCAGCAGC TCCAGCCACA GCCACCGCCA 
CCCCCACCTC CTCAGCCACA GCCACCACCC CAGCAACAAC ACCAGCCTCC ACCACGACCA 
GTTCACTTGC CATCCATGCC CTTTTCTGCT CATATTCAGC AGCCCCCACC ACCCCCAGGA 
CAGCAACCTA CTCACCCACC CCCAGGACAG CAGCCCCCAC CACCACAGCC TGCCAAGCCC 
CAGCAAGTCA TCCAGCATCA CCCTTCCCCC CGGCACCACA AGTCAGACCC CTACTCAGCT 
GGTCATCTTC GTGAGGCTCC CTCTCCGCTT ATGATACATT CCCCTCAGAT GCCACAGTTC 
CAGAGCCTGA CCCATCAGTC TCCTCCCCAG CAAAACGTCC AGCCTAAGAA GCAGGTAAAG 
GGCAGGGCTG AGCCACAGCC ACCAGGGCCA GTCATGGGCC AAGGCCAGGG ATGCCCACCT 
GCCTCACCGG CTGCCGTGCC TATGCTGTCC CAGGAGCTGC GACCACCTTC AGTCGTCCAG 
CCCCAGCCCC TGGTGGTAGT AAAGGAGGAG AAGATTCACT CACCAATCAT TCGCAGCGAG 
CCTTTCAGCA CCTCACTTCG ACCAGAGCCC CCCAAGCACC CAGAGAACAT CAAGGCCCCA 
GTCCACCTGC CCCAGCGGCC TGAGATGAAG CCTGTAGACA TAGGGAGGCC TGTGATCCGG 
CCTCCAGAGC AGAGTGCACC CCCACCAGGG GCCCCTGACA AGGACAAACA GAAGCAGGAG 
CCAAAAACAC CAGTGGCACC CAAAAAGGAC CTGAAAATTA AGAACATGGG CTCCTGGGCC 
AGCCTGGTAC AGAAGCATCC GACCACCCCA TCCTCCACAG CCAAGTCCTC AAGTGACAGC 
TTTGAGCATT TCCGCCGTGC TGCTCGGGAG AAGGAGGAGA GGGAAAAGGC CCTGAAGGCT 
CAGGCTGAGC ATGCAGAGAA GGAGAAGGAG CGGCTGAGGC AGGAGCGCAT GAGGAGCCGA 
GAGGATGAAG ATGCGCTGGA ACAGGCACGT CGAGCCCATG AGGAGGCACG ACGGCGCCAG 
GAGCAGCAGC AGCAGCAGCA GCAGCAGAGA CAGGAGCAGC AGCAGCAGCA GCAGCAGGCA 
GCTGCTGTGG CCGCTGCCTC TGCCCCCCAG GCCCAGAGCT CCCAGCCCCA GTCTATGCTG 
GACCAGCAGA GGGAGTTGGC CCGGAAACGA GAGCAGGAGC GGAGGCGCAG GGAGGCAATG 
GCAGCTACAA TTGACATGAA TTTCCAGAGT GATCTTTTGT CAATATTTGA AGAAAATCTT 
TTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001273559.1
CDS	310..4515
Translation	MSTESGPGTRLRNLPVMGDGLETSQMSTTQAQAQPQPANAASTNPPPPETSNPNKPKRQTNQLQYLLRVVLKTLWKHQFAWPFQQPVDAVKLNLPDYYKIIKTPMDMGTIKKRLENNYYWNAQECIQDFNTMFTNCYIYNKPGDDIVLMAEALEKLFLQKINELPTEETEIMIVQAKGRGRGRKETGRAAKPGVSTVPNTTQASTSPQTQTPQQNPPPPVQATTHPFPAVTPDLIAQPPVMTMVPPQPLQTPSPVPPQPPPPPAPVPQPVQSHPPIIATTPQPVKTKKGVKRKADTTTPTTIDPIHEPPSLAPEPKTAKLGPRRESSRPVKPPKKDVPDSQQHPGPEKSSKISEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYCDIIKHPMDMSTIKSKLESREYRDAQEFGADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPEEPVVTVSSPAVPPPTKVVAPPSSSDSSSDSSSDSDSSTDDSEEERAQRLAELQEQLKAVHEQLAALSQPQQNKPKKKEKDKKEKKKEKHKKKEEVEENKKSKTKELPPKKTKKNNSSNSNVSKKEPVPTKTKPPPTYESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVDVIAGSSKMKGFSSSESESTSESSSSDSEDSETEMAPKSKKKGHTGRDQKKHHHHHHPQMQPAPAPVPQQPPPPPQQPPPPPPPQQQQQQPPPPPPPPSMPQQTAPAMKSSPPPFITAQVPVLEPQLPGSVFDPIGHFTQPILHLPQPELPPHLPQPPEHSTPPHLNQHAVVSPPALHNALPQQPSRPSNRAAALPPKPTRPPAVSPALAQPPLLPQPPMAQPPQVLLEDEEPPAPPLTSMQMQLYLQQLQKVQPPTPLLPSVKVQSQPPPPLPPPPHPSVQQQQLQPQPPPPPPPQPQPPPQQQHQPPPRPVHLPSMPFSAHIQQPPPPPGQQPTHPPPGQQPPPPQPAKPQQVIQHHPSPRHHKSDPYSAGHLREAPSPLMIHSPQMPQFQSLTHQSPPQQNVQPKKQVKGRAEPQPPGPVMGQGQGCPPASPAAVPMLSQELRPPSVVQPQPLVVVKEEKIHSPIIRSEPFSTSLRPEPPKHPENIKAPVHLPQRPEMKPVDIGRPVIRPPEQSAPPPGAPDKDKQKQEPKTPVAPKKDLKIKNMGSWASLVQKHPTTPSSTAKSSSDSFEHFRRAAREKEEREKALKAQAEHAEKEKERLRQERMRSREDEDALEQARRAHEEARRRQEQQQQQQQQRQEQQQQQQQAAAVAAASAPQAQSSQPQSMLDQQRELARKREQERRRREAMAATIDMNFQSDLLSIFEENLF

Target ORF information:

RefSeq Version	NM_001286630.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus bromodomain containing 4 (Brd4), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001286630.1

ORF Insert Sequence:

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGCC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGACAGACA 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCCGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAAAAA 
ATCAATGAAC TGCCTACAGA AGAAACTGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGAGGGAGGA AAGAAACAGG TAGGGCAGCA AAGCCTGGTG TATCCACGGT ACCAAACACA 
ACTCAAGCAT CAACTTCTCC GCAGACCCAG ACGCCTCAGC AGAACCCTCC TCCACCTGTG 
CAGGCCACAA CTCACCCCTT TCCTGCTGTC ACCCCAGACC TCATTGCCCA GCCTCCTGTC 
ATGACAATGG TGCCCCCTCA GCCACTTCAG ACTCCTTCAC CGGTACCCCC CCAGCCACCA 
CCCCCACCTG CTCCAGTTCC ACAGCCTGTG CAGAGTCACC CGCCCATCAT TGCGACCACC 
CCCCAGCCTG TGAAGACAAA GAAAGGGGTG AAGAGGAAAG CAGATACCAC CACCCCTACC 
ACCATCGACC CCATTCATGA GCCACCCTCA CTGGCCCCAG AGCCCAAGAC CGCCAAGCTG 
GGTCCTCGGC GGGAGAGCAG CAGACCTGTG AAGCCTCCAA AGAAGGATGT ACCGGACTCA 
CAGCAGCACC CAGGGCCAGA GAAGAGCAGC AAGATCTCTG AGCAGCTAAA GTGCTGCAGT 
GGCATCCTCA AGGAGATGTT TGCCAAGAAA CATGCTGCCT ATGCCTGGCC TTTCTACAAG 
CCTGTGGATG TGGAGGCACT GGGTCTGCAC GACTACTGTG ACATCATCAA ACATCCCATG 
GACATGAGCA CAATCAAGTC TAAACTAGAG TCCCGAGAGT ACAGAGATGC CCAGGAATTT 
GGTGCTGATG TCCGATTGAT GTTCTCCAAC TGCTACAAGT ACAACCCCCC TGACCATGAA 
GTGGTAGCCA TGGCTCGAAA ACTCCAGGAT GTGTTTGAAA TGCGCTTTGC CAAGATGCCT 
GATGAGCCTG AAGAGCCAGT TGTTACAGTG TCCTCTCCTG CAGTGCCACC CCCTACAAAG 
GTGGTAGCCC CACCCTCATC TAGTGACAGC AGCAGCGACA GTTCTTCCGA CAGCGACAGT 
TCCACTGACG ACTCTGAGGA AGAGCGAGCC CAGCGGCTGG CTGAACTCCA GGAACAGCTC 
AAGGCCGTGC ATGAGCAGCT TGCAGCCCTC TCACAGCCCC AGCAGAACAA ACCAAAGAAA 
AAGGAGAAGG ACAAGAAGGA AAAGAAAAAG GAAAAGCACA AAAAGAAAGA AGAAGTGGAG 
GAAAATAAAA AAAGCAAAAC CAAGGAACTT CCTCCCAAAA AGACAAAGAA AAATAACAGC 
AGCAACAGCA ATGTGAGCAA GAAGGAACCA GTACCCACGA AGACCAAGCC GCCTCCCACA 
TATGAATCAG AAGAGGAGGA TAAGTGTAAG CCCATGTCTT ATGAGGAGAA GCGGCAGCTA 
AGTCTAGATA TCAACAAACT TCCTGGTGAG AAGCTAGGCC GTGTAGTACA CATAATTCAG 
TCAAGGGAAC CATCACTTAA AAACTCCAAC CCCGATGAGA TTGAGATTGA CTTTGAGACC 
CTGAAGCCAT CTACACTACG AGAGTTGGAG CGATATGTCA CCTCCTGTTT GCGGAAGAAA 
AGGAAACCTC AAGCTGAAAA AGTTGACGTG ATTGCTGGTT CTTCCAAGAT GAAGGGATTC 
TCATCCTCTG AGTCGGAGAG CACCAGCGAA TCCAGCTCCT CTGACAGTGA AGACTCTGAA 
ACAGAGATGG CTCCCAAGTC AAAAAAGAAG GGGCACACTG GGAGGGACCA GAAAAAGCAT 
CATCACCATC ACCATCCACA GATGCAGCCA GCCCCAGCTC CTGTGCCCCA GCAGCCGCCC 
CCACCTCCAC AGCAGCCTCC TCCACCCCCA CCTCCGCAGC AGCAGCAGCA GCAACCTCCA 
CCCCCACCAC CTCCGCCCTC CATGCCACAG CAGACTGCCC CAGCGATGAA GTCCTCGCCC 
CCACCCTTCA TCACTGCCCA GGTCCCCGTC CTGGAACCAC AGCTGCCAGG CAGTGTCTTT 
GACCCTATTG GCCACTTCAC CCAGCCCATC TTGCACCTGC CGCAGCCGGA GCTGCCTCCT 
CACCTGCCCC AGCCACCTGA GCACAGCACT CCACCCCATC TCAACCAGCA TGCTGTGGTC 
TCTCCTCCAG CTTTGCACAA TGCGCTGCCC CAACAGCCAT CTCGGCCCAG TAACCGAGCT 
GCTGCTCTGC CCCCAAAGCC TACCCGACCC CCAGCTGTGT CCCCTGCCCT GGCCCAGCCC 
CCCCTGCTCC CACAACCACC AATGGCTCAG CCCCCCCAAG TGCTGCTGGA GGATGAAGAG 
CCACCTGCCC CACCCCTCAC CTCCATGCAG ATGCAGTTGT ACCTACAGCA GCTGCAGAAG 
GTGCAGCCTC CCACACCACT ACTCCCTTCC GTGAAGGTGC AGTCCCAACC CCCACCGCCT 
TTGCCGCCTC CACCCCACCC TTCTGTGCAG CAGCAGCAGC TCCAGCCACA GCCACCGCCA 
CCCCCACCTC CTCAGCCACA GCCACCACCC CAGCAACAAC ACCAGCCTCC ACCACGACCA 
GTTCACTTGC CATCCATGCC CTTTTCTGCT CATATTCAGC AGCCCCCACC ACCCCCAGGA 
CAGCAACCTA CTCACCCACC CCCAGGACAG CAGCCCCCAC CACCACAGCC TGCCAAGCCC 
CAGCAAGTCA TCCAGCATCA CCCTTCCCCC CGGCACCACA AGTCAGACCC CTACTCAGCT 
GGTCATCTTC GTGAGGCTCC CTCTCCGCTT ATGATACATT CCCCTCAGAT GCCACAGTTC 
CAGAGCCTGA CCCATCAGTC TCCTCCCCAG CAAAACGTCC AGCCTAAGAA GCAGGTAAAG 
GGCAGGGCTG AGCCACAGCC ACCAGGGCCA GTCATGGGCC AAGGCCAGGG ATGCCCACCT 
GCCTCACCGG CTGCCGTGCC TATGCTGTCC CAGGAGCTGC GACCACCTTC AGTCGTCCAG 
CCCCAGCCCC TGGTGGTAGT AAAGGAGGAG AAGATTCACT CACCAATCAT TCGCAGCGAG 
CCTTTCAGCA CCTCACTTCG ACCAGAGCCC CCCAAGCACC CAGAGAACAT CAAGGCCCCA 
GTCCACCTGC CCCAGCGGCC TGAGATGAAG CCTGTAGACA TAGGGAGGCC TGTGATCCGG 
CCTCCAGAGC AGAGTGCACC CCCACCAGGG GCCCCTGACA AGGACAAACA GAAGCAGGAG 
CCAAAAACAC CAGTGGCACC CAAAAAGGAC CTGAAAATTA AGAACATGGG CTCCTGGGCC 
AGCCTGGTAC AGAAGCATCC GACCACCCCA TCCTCCACAG CCAAGTCCTC AAGTGACAGC 
TTTGAGCATT TCCGCCGTGC TGCTCGGGAG AAGGAGGAGA GGGAAAAGGC CCTGAAGGCT 
CAGGCTGAGC ATGCAGAGAA GGAGAAGGAG CGGCTGAGGC AGGAGCGCAT GAGGAGCCGA 
GAGGATGAAG ATGCGCTGGA ACAGGCACGT CGAGCCCATG AGGAGGCACG ACGGCGCCAG 
GAGCAGCAGC AGCAGCAGCA GCAGCAGAGA CAGGAGCAGC AGCAGCAGCA GCAGCAGGCA 
GCTGCTGTGG CCGCTGCCTC TGCCCCCCAG GCCCAGAGCT CCCAGCCCCA GTCTATGCTG 
GACCAGCAGA GGGAGTTGGC CCGGAAACGA GAGCAGGAGC GGAGGCGCAG GGAGGCAATG 
GCAGCTACAA TTGACATGAA TTTCCAGAGT GATCTTTTGT CAATATTTGA AGAAAATCTT 
TTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brd4 ( XM_030249927.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105787.1 Mus musculus bromodomain containing 4 (Brd4), transcript variant X12, mRNA.

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CloneID	OMu16383
Clone ID Related Accession (Same CDS sequence)	XM_030249927.1 , XM_030249925.1 , XM_006524689.2 , NM_020508.4 , XM_006524688.1 , XM_006524688.2 , XM_030249926.1 , XM_006524689.3 , XM_030249924.1
Accession Version	XM_030249927.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4203bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	bromodomain-containing protein 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGCC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGACAGACA 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCCGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAAAAA 
ATCAATGAAC TGCCTACAGA AGAAACTGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGAGGGAGGA AAGAAACAGG GGCAGCAAAG CCTGGTGTAT CCACGGTACC AAACACAACT 
CAAGCATCAA CTTCTCCGCA GACCCAGACG CCTCAGCAGA ACCCTCCTCC ACCTGTGCAG 
GCCACAACTC ACCCCTTTCC TGCTGTCACC CCAGACCTCA TTGCCCAGCC TCCTGTCATG 
ACAATGGTGC CCCCTCAGCC ACTTCAGACT CCTTCACCGG TACCCCCCCA GCCACCACCC 
CCACCTGCTC CAGTTCCACA GCCTGTGCAG AGTCACCCGC CCATCATTGC GACCACCCCC 
CAGCCTGTGA AGACAAAGAA AGGGGTGAAG AGGAAAGCAG ATACCACCAC CCCTACCACC 
ATCGACCCCA TTCATGAGCC ACCCTCACTG GCCCCAGAGC CCAAGACCGC CAAGCTGGGT 
CCTCGGCGGG AGAGCAGCAG ACCTGTGAAG CCTCCAAAGA AGGATGTACC GGACTCACAG 
CAGCACCCAG GGCCAGAGAA GAGCAGCAAG ATCTCTGAGC AGCTAAAGTG CTGCAGTGGC 
ATCCTCAAGG AGATGTTTGC CAAGAAACAT GCTGCCTATG CCTGGCCTTT CTACAAGCCT 
GTGGATGTGG AGGCACTGGG TCTGCACGAC TACTGTGACA TCATCAAACA TCCCATGGAC 
ATGAGCACAA TCAAGTCTAA ACTAGAGTCC CGAGAGTACA GAGATGCCCA GGAATTTGGT 
GCTGATGTCC GATTGATGTT CTCCAACTGC TACAAGTACA ACCCCCCTGA CCATGAAGTG 
GTAGCCATGG CTCGAAAACT CCAGGATGTG TTTGAAATGC GCTTTGCCAA GATGCCTGAT 
GAGCCTGAAG AGCCAGTTGT TACAGTGTCC TCTCCTGCAG TGCCACCCCC TACAAAGGTG 
GTAGCCCCAC CCTCATCTAG TGACAGCAGC AGCGACAGTT CTTCCGACAG CGACAGTTCC 
ACTGACGACT CTGAGGAAGA GCGAGCCCAG CGGCTGGCTG AACTCCAGGA ACAGCTCAAG 
GCCGTGCATG AGCAGCTTGC AGCCCTCTCA CAGCCCCAGC AGAACAAACC AAAGAAAAAG 
GAGAAGGACA AGAAGGAAAA GAAAAAGGAA AAGCACAAAA AGAAAGAAGA AGTGGAGGAA 
AATAAAAAAA GCAAAACCAA GGAACTTCCT CCCAAAAAGA CAAAGAAAAA TAACAGCAGC 
AACAGCAATG TGAGCAAGAA GGAACCAGTA CCCACGAAGA CCAAGCCGCC TCCCACATAT 
GAATCAGAAG AGGAGGATAA GTGTAAGCCC ATGTCTTATG AGGAGAAGCG GCAGCTAAGT 
CTAGATATCA ACAAACTTCC TGGTGAGAAG CTAGGCCGTG TAGTACACAT AATTCAGTCA 
AGGGAACCAT CACTTAAAAA CTCCAACCCC GATGAGATTG AGATTGACTT TGAGACCCTG 
AAGCCATCTA CACTACGAGA GTTGGAGCGA TATGTCACCT CCTGTTTGCG GAAGAAAAGG 
AAACCTCAAG CTGAAAAAGT TGACGTGATT GCTGGTTCTT CCAAGATGAA GGGATTCTCA 
TCCTCTGAGT CGGAGAGCAC CAGCGAATCC AGCTCCTCTG ACAGTGAAGA CTCTGAAACA 
GAGATGGCTC CCAAGTCAAA AAAGAAGGGG CACACTGGGA GGGACCAGAA AAAGCATCAT 
CACCATCACC ATCCACAGAT GCAGCCAGCC CCAGCTCCTG TGCCCCAGCA GCCGCCCCCA 
CCTCCACAGC AGCCTCCTCC ACCCCCACCT CCGCAGCAGC AGCAGCAGCA ACCTCCACCC 
CCACCACCTC CGCCCTCCAT GCCACAGCAG ACTGCCCCAG CGATGAAGTC CTCGCCCCCA 
CCCTTCATCA CTGCCCAGGT CCCCGTCCTG GAACCACAGC TGCCAGGCAG TGTCTTTGAC 
CCTATTGGCC ACTTCACCCA GCCCATCTTG CACCTGCCGC AGCCGGAGCT GCCTCCTCAC 
CTGCCCCAGC CACCTGAGCA CAGCACTCCA CCCCATCTCA ACCAGCATGC TGTGGTCTCT 
CCTCCAGCTT TGCACAATGC GCTGCCCCAA CAGCCATCTC GGCCCAGTAA CCGAGCTGCT 
GCTCTGCCCC CAAAGCCTAC CCGACCCCCA GCTGTGTCCC CTGCCCTGGC CCAGCCCCCC 
CTGCTCCCAC AACCACCAAT GGCTCAGCCC CCCCAAGTGC TGCTGGAGGA TGAAGAGCCA 
CCTGCCCCAC CCCTCACCTC CATGCAGATG CAGTTGTACC TACAGCAGCT GCAGAAGGTG 
CAGCCTCCCA CACCACTACT CCCTTCCGTG AAGGTGCAGT CCCAACCCCC ACCGCCTTTG 
CCGCCTCCAC CCCACCCTTC TGTGCAGCAG CAGCAGCTCC AGCCACAGCC ACCGCCACCC 
CCACCTCCTC AGCCACAGCC ACCACCCCAG CAACAACACC AGCCTCCACC ACGACCAGTT 
CACTTGCCAT CCATGCCCTT TTCTGCTCAT ATTCAGCAGC CCCCACCACC CCCAGGACAG 
CAACCTACTC ACCCACCCCC AGGACAGCAG CCCCCACCAC CACAGCCTGC CAAGCCCCAG 
CAAGTCATCC AGCATCACCC TTCCCCCCGG CACCACAAGT CAGACCCCTA CTCAGCTGGT 
CATCTTCGTG AGGCTCCCTC TCCGCTTATG ATACATTCCC CTCAGATGCC ACAGTTCCAG 
AGCCTGACCC ATCAGTCTCC TCCCCAGCAA AACGTCCAGC CTAAGAAGCA GGTAAAGGGC 
AGGGCTGAGC CACAGCCACC AGGGCCAGTC ATGGGCCAAG GCCAGGGATG CCCACCTGCC 
TCACCGGCTG CCGTGCCTAT GCTGTCCCAG GAGCTGCGAC CACCTTCAGT CGTCCAGCCC 
CAGCCCCTGG TGGTAGTAAA GGAGGAGAAG ATTCACTCAC CAATCATTCG CAGCGAGCCT 
TTCAGCACCT CACTTCGACC AGAGCCCCCC AAGCACCCAG AGAACATCAA GGCCCCAGTC 
CACCTGCCCC AGCGGCCTGA GATGAAGCCT GTAGACATAG GGAGGCCTGT GATCCGGCCT 
CCAGAGCAGA GTGCACCCCC ACCAGGGGCC CCTGACAAGG ACAAACAGAA GCAGGAGCCA 
AAAACACCAG TGGCACCCAA AAAGGACCTG AAAATTAAGA ACATGGGCTC CTGGGCCAGC 
CTGGTACAGA AGCATCCGAC CACCCCATCC TCCACAGCCA AGTCCTCAAG TGACAGCTTT 
GAGCATTTCC GCCGTGCTGC TCGGGAGAAG GAGGAGAGGG AAAAGGCCCT GAAGGCTCAG 
GCTGAGCATG CAGAGAAGGA GAAGGAGCGG CTGAGGCAGG AGCGCATGAG GAGCCGAGAG 
GATGAAGATG CGCTGGAACA GGCACGTCGA GCCCATGAGG AGGCACGACG GCGCCAGGAG 
CAGCAGCAGC AGCAGCAGCA GCAGAGACAG GAGCAGCAGC AGCAGCAGCA GCAGGCAGCT 
GCTGTGGCCG CTGCCTCTGC CCCCCAGGCC CAGAGCTCCC AGCCCCAGTC TATGCTGGAC 
CAGCAGAGGG AGTTGGCCCG GAAACGAGAG CAGGAGCGGA GGCGCAGGGA GGCAATGGCA 
GCTACAATTG ACATGAATTT CCAGAGTGAT CTTTTGTCAA TATTTGAAGA AAATCTTTTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105787.1
CDS	136..4338
Translation	MSTESGPGTRLRNLPVMGDGLETSQMSTTQAQAQPQPANAASTNPPPPETSNPNKPKRQTNQLQYLLRVVLKTLWKHQFAWPFQQPVDAVKLNLPDYYKIIKTPMDMGTIKKRLENNYYWNAQECIQDFNTMFTNCYIYNKPGDDIVLMAEALEKLFLQKINELPTEETEIMIVQAKGRGRGRKETGAAKPGVSTVPNTTQASTSPQTQTPQQNPPPPVQATTHPFPAVTPDLIAQPPVMTMVPPQPLQTPSPVPPQPPPPPAPVPQPVQSHPPIIATTPQPVKTKKGVKRKADTTTPTTIDPIHEPPSLAPEPKTAKLGPRRESSRPVKPPKKDVPDSQQHPGPEKSSKISEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYCDIIKHPMDMSTIKSKLESREYRDAQEFGADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPEEPVVTVSSPAVPPPTKVVAPPSSSDSSSDSSSDSDSSTDDSEEERAQRLAELQEQLKAVHEQLAALSQPQQNKPKKKEKDKKEKKKEKHKKKEEVEENKKSKTKELPPKKTKKNNSSNSNVSKKEPVPTKTKPPPTYESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVDVIAGSSKMKGFSSSESESTSESSSSDSEDSETEMAPKSKKKGHTGRDQKKHHHHHHPQMQPAPAPVPQQPPPPPQQPPPPPPPQQQQQQPPPPPPPPSMPQQTAPAMKSSPPPFITAQVPVLEPQLPGSVFDPIGHFTQPILHLPQPELPPHLPQPPEHSTPPHLNQHAVVSPPALHNALPQQPSRPSNRAAALPPKPTRPPAVSPALAQPPLLPQPPMAQPPQVLLEDEEPPAPPLTSMQMQLYLQQLQKVQPPTPLLPSVKVQSQPPPPLPPPPHPSVQQQQLQPQPPPPPPPQPQPPPQQQHQPPPRPVHLPSMPFSAHIQQPPPPPGQQPTHPPPGQQPPPPQPAKPQQVIQHHPSPRHHKSDPYSAGHLREAPSPLMIHSPQMPQFQSLTHQSPPQQNVQPKKQVKGRAEPQPPGPVMGQGQGCPPASPAAVPMLSQELRPPSVVQPQPLVVVKEEKIHSPIIRSEPFSTSLRPEPPKHPENIKAPVHLPQRPEMKPVDIGRPVIRPPEQSAPPPGAPDKDKQKQEPKTPVAPKKDLKIKNMGSWASLVQKHPTTPSSTAKSSSDSFEHFRRAAREKEEREKALKAQAEHAEKEKERLRQERMRSREDEDALEQARRAHEEARRRQEQQQQQQQQRQEQQQQQQQAAAVAAASAPQAQSSQPQSMLDQQRELARKREQERRRREAMAATIDMNFQSDLLSIFEENLF

Target ORF information:

RefSeq Version	XM_030249927.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus bromodomain containing 4 (Brd4), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249927.1

ORF Insert Sequence:

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGCC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGACAGACA 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCCGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAAAAA 
ATCAATGAAC TGCCTACAGA AGAAACTGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGAGGGAGGA AAGAAACAGG GGCAGCAAAG CCTGGTGTAT CCACGGTACC AAACACAACT 
CAAGCATCAA CTTCTCCGCA GACCCAGACG CCTCAGCAGA ACCCTCCTCC ACCTGTGCAG 
GCCACAACTC ACCCCTTTCC TGCTGTCACC CCAGACCTCA TTGCCCAGCC TCCTGTCATG 
ACAATGGTGC CCCCTCAGCC ACTTCAGACT CCTTCACCGG TACCCCCCCA GCCACCACCC 
CCACCTGCTC CAGTTCCACA GCCTGTGCAG AGTCACCCGC CCATCATTGC GACCACCCCC 
CAGCCTGTGA AGACAAAGAA AGGGGTGAAG AGGAAAGCAG ATACCACCAC CCCTACCACC 
ATCGACCCCA TTCATGAGCC ACCCTCACTG GCCCCAGAGC CCAAGACCGC CAAGCTGGGT 
CCTCGGCGGG AGAGCAGCAG ACCTGTGAAG CCTCCAAAGA AGGATGTACC GGACTCACAG 
CAGCACCCAG GGCCAGAGAA GAGCAGCAAG ATCTCTGAGC AGCTAAAGTG CTGCAGTGGC 
ATCCTCAAGG AGATGTTTGC CAAGAAACAT GCTGCCTATG CCTGGCCTTT CTACAAGCCT 
GTGGATGTGG AGGCACTGGG TCTGCACGAC TACTGTGACA TCATCAAACA TCCCATGGAC 
ATGAGCACAA TCAAGTCTAA ACTAGAGTCC CGAGAGTACA GAGATGCCCA GGAATTTGGT 
GCTGATGTCC GATTGATGTT CTCCAACTGC TACAAGTACA ACCCCCCTGA CCATGAAGTG 
GTAGCCATGG CTCGAAAACT CCAGGATGTG TTTGAAATGC GCTTTGCCAA GATGCCTGAT 
GAGCCTGAAG AGCCAGTTGT TACAGTGTCC TCTCCTGCAG TGCCACCCCC TACAAAGGTG 
GTAGCCCCAC CCTCATCTAG TGACAGCAGC AGCGACAGTT CTTCCGACAG CGACAGTTCC 
ACTGACGACT CTGAGGAAGA GCGAGCCCAG CGGCTGGCTG AACTCCAGGA ACAGCTCAAG 
GCCGTGCATG AGCAGCTTGC AGCCCTCTCA CAGCCCCAGC AGAACAAACC AAAGAAAAAG 
GAGAAGGACA AGAAGGAAAA GAAAAAGGAA AAGCACAAAA AGAAAGAAGA AGTGGAGGAA 
AATAAAAAAA GCAAAACCAA GGAACTTCCT CCCAAAAAGA CAAAGAAAAA TAACAGCAGC 
AACAGCAATG TGAGCAAGAA GGAACCAGTA CCCACGAAGA CCAAGCCGCC TCCCACATAT 
GAATCAGAAG AGGAGGATAA GTGTAAGCCC ATGTCTTATG AGGAGAAGCG GCAGCTAAGT 
CTAGATATCA ACAAACTTCC TGGTGAGAAG CTAGGCCGTG TAGTACACAT AATTCAGTCA 
AGGGAACCAT CACTTAAAAA CTCCAACCCC GATGAGATTG AGATTGACTT TGAGACCCTG 
AAGCCATCTA CACTACGAGA GTTGGAGCGA TATGTCACCT CCTGTTTGCG GAAGAAAAGG 
AAACCTCAAG CTGAAAAAGT TGACGTGATT GCTGGTTCTT CCAAGATGAA GGGATTCTCA 
TCCTCTGAGT CGGAGAGCAC CAGCGAATCC AGCTCCTCTG ACAGTGAAGA CTCTGAAACA 
GAGATGGCTC CCAAGTCAAA AAAGAAGGGG CACACTGGGA GGGACCAGAA AAAGCATCAT 
CACCATCACC ATCCACAGAT GCAGCCAGCC CCAGCTCCTG TGCCCCAGCA GCCGCCCCCA 
CCTCCACAGC AGCCTCCTCC ACCCCCACCT CCGCAGCAGC AGCAGCAGCA ACCTCCACCC 
CCACCACCTC CGCCCTCCAT GCCACAGCAG ACTGCCCCAG CGATGAAGTC CTCGCCCCCA 
CCCTTCATCA CTGCCCAGGT CCCCGTCCTG GAACCACAGC TGCCAGGCAG TGTCTTTGAC 
CCTATTGGCC ACTTCACCCA GCCCATCTTG CACCTGCCGC AGCCGGAGCT GCCTCCTCAC 
CTGCCCCAGC CACCTGAGCA CAGCACTCCA CCCCATCTCA ACCAGCATGC TGTGGTCTCT 
CCTCCAGCTT TGCACAATGC GCTGCCCCAA CAGCCATCTC GGCCCAGTAA CCGAGCTGCT 
GCTCTGCCCC CAAAGCCTAC CCGACCCCCA GCTGTGTCCC CTGCCCTGGC CCAGCCCCCC 
CTGCTCCCAC AACCACCAAT GGCTCAGCCC CCCCAAGTGC TGCTGGAGGA TGAAGAGCCA 
CCTGCCCCAC CCCTCACCTC CATGCAGATG CAGTTGTACC TACAGCAGCT GCAGAAGGTG 
CAGCCTCCCA CACCACTACT CCCTTCCGTG AAGGTGCAGT CCCAACCCCC ACCGCCTTTG 
CCGCCTCCAC CCCACCCTTC TGTGCAGCAG CAGCAGCTCC AGCCACAGCC ACCGCCACCC 
CCACCTCCTC AGCCACAGCC ACCACCCCAG CAACAACACC AGCCTCCACC ACGACCAGTT 
CACTTGCCAT CCATGCCCTT TTCTGCTCAT ATTCAGCAGC CCCCACCACC CCCAGGACAG 
CAACCTACTC ACCCACCCCC AGGACAGCAG CCCCCACCAC CACAGCCTGC CAAGCCCCAG 
CAAGTCATCC AGCATCACCC TTCCCCCCGG CACCACAAGT CAGACCCCTA CTCAGCTGGT 
CATCTTCGTG AGGCTCCCTC TCCGCTTATG ATACATTCCC CTCAGATGCC ACAGTTCCAG 
AGCCTGACCC ATCAGTCTCC TCCCCAGCAA AACGTCCAGC CTAAGAAGCA GGTAAAGGGC 
AGGGCTGAGC CACAGCCACC AGGGCCAGTC ATGGGCCAAG GCCAGGGATG CCCACCTGCC 
TCACCGGCTG CCGTGCCTAT GCTGTCCCAG GAGCTGCGAC CACCTTCAGT CGTCCAGCCC 
CAGCCCCTGG TGGTAGTAAA GGAGGAGAAG ATTCACTCAC CAATCATTCG CAGCGAGCCT 
TTCAGCACCT CACTTCGACC AGAGCCCCCC AAGCACCCAG AGAACATCAA GGCCCCAGTC 
CACCTGCCCC AGCGGCCTGA GATGAAGCCT GTAGACATAG GGAGGCCTGT GATCCGGCCT 
CCAGAGCAGA GTGCACCCCC ACCAGGGGCC CCTGACAAGG ACAAACAGAA GCAGGAGCCA 
AAAACACCAG TGGCACCCAA AAAGGACCTG AAAATTAAGA ACATGGGCTC CTGGGCCAGC 
CTGGTACAGA AGCATCCGAC CACCCCATCC TCCACAGCCA AGTCCTCAAG TGACAGCTTT 
GAGCATTTCC GCCGTGCTGC TCGGGAGAAG GAGGAGAGGG AAAAGGCCCT GAAGGCTCAG 
GCTGAGCATG CAGAGAAGGA GAAGGAGCGG CTGAGGCAGG AGCGCATGAG GAGCCGAGAG 
GATGAAGATG CGCTGGAACA GGCACGTCGA GCCCATGAGG AGGCACGACG GCGCCAGGAG 
CAGCAGCAGC AGCAGCAGCA GCAGAGACAG GAGCAGCAGC AGCAGCAGCA GCAGGCAGCT 
GCTGTGGCCG CTGCCTCTGC CCCCCAGGCC CAGAGCTCCC AGCCCCAGTC TATGCTGGAC 
CAGCAGAGGG AGTTGGCCCG GAAACGAGAG CAGGAGCGGA GGCGCAGGGA GGCAATGGCA 
GCTACAATTG ACATGAATTT CCAGAGTGAT CTTTTGTCAA TATTTGAAGA AAATCTTTTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brd4 ( XM_030249925.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105785.1 Mus musculus bromodomain containing 4 (Brd4), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OMu16383
Clone ID Related Accession (Same CDS sequence)	XM_030249927.1 , XM_030249925.1 , XM_006524689.2 , NM_020508.4 , XM_006524688.1 , XM_006524688.2 , XM_030249926.1 , XM_006524689.3 , XM_030249924.1
Accession Version	XM_030249925.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4203bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	bromodomain-containing protein 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGCC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGACAGACA 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCCGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAAAAA 
ATCAATGAAC TGCCTACAGA AGAAACTGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGAGGGAGGA AAGAAACAGG GGCAGCAAAG CCTGGTGTAT CCACGGTACC AAACACAACT 
CAAGCATCAA CTTCTCCGCA GACCCAGACG CCTCAGCAGA ACCCTCCTCC ACCTGTGCAG 
GCCACAACTC ACCCCTTTCC TGCTGTCACC CCAGACCTCA TTGCCCAGCC TCCTGTCATG 
ACAATGGTGC CCCCTCAGCC ACTTCAGACT CCTTCACCGG TACCCCCCCA GCCACCACCC 
CCACCTGCTC CAGTTCCACA GCCTGTGCAG AGTCACCCGC CCATCATTGC GACCACCCCC 
CAGCCTGTGA AGACAAAGAA AGGGGTGAAG AGGAAAGCAG ATACCACCAC CCCTACCACC 
ATCGACCCCA TTCATGAGCC ACCCTCACTG GCCCCAGAGC CCAAGACCGC CAAGCTGGGT 
CCTCGGCGGG AGAGCAGCAG ACCTGTGAAG CCTCCAAAGA AGGATGTACC GGACTCACAG 
CAGCACCCAG GGCCAGAGAA GAGCAGCAAG ATCTCTGAGC AGCTAAAGTG CTGCAGTGGC 
ATCCTCAAGG AGATGTTTGC CAAGAAACAT GCTGCCTATG CCTGGCCTTT CTACAAGCCT 
GTGGATGTGG AGGCACTGGG TCTGCACGAC TACTGTGACA TCATCAAACA TCCCATGGAC 
ATGAGCACAA TCAAGTCTAA ACTAGAGTCC CGAGAGTACA GAGATGCCCA GGAATTTGGT 
GCTGATGTCC GATTGATGTT CTCCAACTGC TACAAGTACA ACCCCCCTGA CCATGAAGTG 
GTAGCCATGG CTCGAAAACT CCAGGATGTG TTTGAAATGC GCTTTGCCAA GATGCCTGAT 
GAGCCTGAAG AGCCAGTTGT TACAGTGTCC TCTCCTGCAG TGCCACCCCC TACAAAGGTG 
GTAGCCCCAC CCTCATCTAG TGACAGCAGC AGCGACAGTT CTTCCGACAG CGACAGTTCC 
ACTGACGACT CTGAGGAAGA GCGAGCCCAG CGGCTGGCTG AACTCCAGGA ACAGCTCAAG 
GCCGTGCATG AGCAGCTTGC AGCCCTCTCA CAGCCCCAGC AGAACAAACC AAAGAAAAAG 
GAGAAGGACA AGAAGGAAAA GAAAAAGGAA AAGCACAAAA AGAAAGAAGA AGTGGAGGAA 
AATAAAAAAA GCAAAACCAA GGAACTTCCT CCCAAAAAGA CAAAGAAAAA TAACAGCAGC 
AACAGCAATG TGAGCAAGAA GGAACCAGTA CCCACGAAGA CCAAGCCGCC TCCCACATAT 
GAATCAGAAG AGGAGGATAA GTGTAAGCCC ATGTCTTATG AGGAGAAGCG GCAGCTAAGT 
CTAGATATCA ACAAACTTCC TGGTGAGAAG CTAGGCCGTG TAGTACACAT AATTCAGTCA 
AGGGAACCAT CACTTAAAAA CTCCAACCCC GATGAGATTG AGATTGACTT TGAGACCCTG 
AAGCCATCTA CACTACGAGA GTTGGAGCGA TATGTCACCT CCTGTTTGCG GAAGAAAAGG 
AAACCTCAAG CTGAAAAAGT TGACGTGATT GCTGGTTCTT CCAAGATGAA GGGATTCTCA 
TCCTCTGAGT CGGAGAGCAC CAGCGAATCC AGCTCCTCTG ACAGTGAAGA CTCTGAAACA 
GAGATGGCTC CCAAGTCAAA AAAGAAGGGG CACACTGGGA GGGACCAGAA AAAGCATCAT 
CACCATCACC ATCCACAGAT GCAGCCAGCC CCAGCTCCTG TGCCCCAGCA GCCGCCCCCA 
CCTCCACAGC AGCCTCCTCC ACCCCCACCT CCGCAGCAGC AGCAGCAGCA ACCTCCACCC 
CCACCACCTC CGCCCTCCAT GCCACAGCAG ACTGCCCCAG CGATGAAGTC CTCGCCCCCA 
CCCTTCATCA CTGCCCAGGT CCCCGTCCTG GAACCACAGC TGCCAGGCAG TGTCTTTGAC 
CCTATTGGCC ACTTCACCCA GCCCATCTTG CACCTGCCGC AGCCGGAGCT GCCTCCTCAC 
CTGCCCCAGC CACCTGAGCA CAGCACTCCA CCCCATCTCA ACCAGCATGC TGTGGTCTCT 
CCTCCAGCTT TGCACAATGC GCTGCCCCAA CAGCCATCTC GGCCCAGTAA CCGAGCTGCT 
GCTCTGCCCC CAAAGCCTAC CCGACCCCCA GCTGTGTCCC CTGCCCTGGC CCAGCCCCCC 
CTGCTCCCAC AACCACCAAT GGCTCAGCCC CCCCAAGTGC TGCTGGAGGA TGAAGAGCCA 
CCTGCCCCAC CCCTCACCTC CATGCAGATG CAGTTGTACC TACAGCAGCT GCAGAAGGTG 
CAGCCTCCCA CACCACTACT CCCTTCCGTG AAGGTGCAGT CCCAACCCCC ACCGCCTTTG 
CCGCCTCCAC CCCACCCTTC TGTGCAGCAG CAGCAGCTCC AGCCACAGCC ACCGCCACCC 
CCACCTCCTC AGCCACAGCC ACCACCCCAG CAACAACACC AGCCTCCACC ACGACCAGTT 
CACTTGCCAT CCATGCCCTT TTCTGCTCAT ATTCAGCAGC CCCCACCACC CCCAGGACAG 
CAACCTACTC ACCCACCCCC AGGACAGCAG CCCCCACCAC CACAGCCTGC CAAGCCCCAG 
CAAGTCATCC AGCATCACCC TTCCCCCCGG CACCACAAGT CAGACCCCTA CTCAGCTGGT 
CATCTTCGTG AGGCTCCCTC TCCGCTTATG ATACATTCCC CTCAGATGCC ACAGTTCCAG 
AGCCTGACCC ATCAGTCTCC TCCCCAGCAA AACGTCCAGC CTAAGAAGCA GGTAAAGGGC 
AGGGCTGAGC CACAGCCACC AGGGCCAGTC ATGGGCCAAG GCCAGGGATG CCCACCTGCC 
TCACCGGCTG CCGTGCCTAT GCTGTCCCAG GAGCTGCGAC CACCTTCAGT CGTCCAGCCC 
CAGCCCCTGG TGGTAGTAAA GGAGGAGAAG ATTCACTCAC CAATCATTCG CAGCGAGCCT 
TTCAGCACCT CACTTCGACC AGAGCCCCCC AAGCACCCAG AGAACATCAA GGCCCCAGTC 
CACCTGCCCC AGCGGCCTGA GATGAAGCCT GTAGACATAG GGAGGCCTGT GATCCGGCCT 
CCAGAGCAGA GTGCACCCCC ACCAGGGGCC CCTGACAAGG ACAAACAGAA GCAGGAGCCA 
AAAACACCAG TGGCACCCAA AAAGGACCTG AAAATTAAGA ACATGGGCTC CTGGGCCAGC 
CTGGTACAGA AGCATCCGAC CACCCCATCC TCCACAGCCA AGTCCTCAAG TGACAGCTTT 
GAGCATTTCC GCCGTGCTGC TCGGGAGAAG GAGGAGAGGG AAAAGGCCCT GAAGGCTCAG 
GCTGAGCATG CAGAGAAGGA GAAGGAGCGG CTGAGGCAGG AGCGCATGAG GAGCCGAGAG 
GATGAAGATG CGCTGGAACA GGCACGTCGA GCCCATGAGG AGGCACGACG GCGCCAGGAG 
CAGCAGCAGC AGCAGCAGCA GCAGAGACAG GAGCAGCAGC AGCAGCAGCA GCAGGCAGCT 
GCTGTGGCCG CTGCCTCTGC CCCCCAGGCC CAGAGCTCCC AGCCCCAGTC TATGCTGGAC 
CAGCAGAGGG AGTTGGCCCG GAAACGAGAG CAGGAGCGGA GGCGCAGGGA GGCAATGGCA 
GCTACAATTG ACATGAATTT CCAGAGTGAT CTTTTGTCAA TATTTGAAGA AAATCTTTTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105785.1
CDS	478..4680
Translation	MSTESGPGTRLRNLPVMGDGLETSQMSTTQAQAQPQPANAASTNPPPPETSNPNKPKRQTNQLQYLLRVVLKTLWKHQFAWPFQQPVDAVKLNLPDYYKIIKTPMDMGTIKKRLENNYYWNAQECIQDFNTMFTNCYIYNKPGDDIVLMAEALEKLFLQKINELPTEETEIMIVQAKGRGRGRKETGAAKPGVSTVPNTTQASTSPQTQTPQQNPPPPVQATTHPFPAVTPDLIAQPPVMTMVPPQPLQTPSPVPPQPPPPPAPVPQPVQSHPPIIATTPQPVKTKKGVKRKADTTTPTTIDPIHEPPSLAPEPKTAKLGPRRESSRPVKPPKKDVPDSQQHPGPEKSSKISEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYCDIIKHPMDMSTIKSKLESREYRDAQEFGADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPEEPVVTVSSPAVPPPTKVVAPPSSSDSSSDSSSDSDSSTDDSEEERAQRLAELQEQLKAVHEQLAALSQPQQNKPKKKEKDKKEKKKEKHKKKEEVEENKKSKTKELPPKKTKKNNSSNSNVSKKEPVPTKTKPPPTYESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVDVIAGSSKMKGFSSSESESTSESSSSDSEDSETEMAPKSKKKGHTGRDQKKHHHHHHPQMQPAPAPVPQQPPPPPQQPPPPPPPQQQQQQPPPPPPPPSMPQQTAPAMKSSPPPFITAQVPVLEPQLPGSVFDPIGHFTQPILHLPQPELPPHLPQPPEHSTPPHLNQHAVVSPPALHNALPQQPSRPSNRAAALPPKPTRPPAVSPALAQPPLLPQPPMAQPPQVLLEDEEPPAPPLTSMQMQLYLQQLQKVQPPTPLLPSVKVQSQPPPPLPPPPHPSVQQQQLQPQPPPPPPPQPQPPPQQQHQPPPRPVHLPSMPFSAHIQQPPPPPGQQPTHPPPGQQPPPPQPAKPQQVIQHHPSPRHHKSDPYSAGHLREAPSPLMIHSPQMPQFQSLTHQSPPQQNVQPKKQVKGRAEPQPPGPVMGQGQGCPPASPAAVPMLSQELRPPSVVQPQPLVVVKEEKIHSPIIRSEPFSTSLRPEPPKHPENIKAPVHLPQRPEMKPVDIGRPVIRPPEQSAPPPGAPDKDKQKQEPKTPVAPKKDLKIKNMGSWASLVQKHPTTPSSTAKSSSDSFEHFRRAAREKEEREKALKAQAEHAEKEKERLRQERMRSREDEDALEQARRAHEEARRRQEQQQQQQQQRQEQQQQQQQAAAVAAASAPQAQSSQPQSMLDQQRELARKREQERRRREAMAATIDMNFQSDLLSIFEENLF

Target ORF information:

RefSeq Version	XM_030249925.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus bromodomain containing 4 (Brd4), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249925.1

ORF Insert Sequence:

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGCC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGACAGACA 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCCGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAAAAA 
ATCAATGAAC TGCCTACAGA AGAAACTGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGAGGGAGGA AAGAAACAGG GGCAGCAAAG CCTGGTGTAT CCACGGTACC AAACACAACT 
CAAGCATCAA CTTCTCCGCA GACCCAGACG CCTCAGCAGA ACCCTCCTCC ACCTGTGCAG 
GCCACAACTC ACCCCTTTCC TGCTGTCACC CCAGACCTCA TTGCCCAGCC TCCTGTCATG 
ACAATGGTGC CCCCTCAGCC ACTTCAGACT CCTTCACCGG TACCCCCCCA GCCACCACCC 
CCACCTGCTC CAGTTCCACA GCCTGTGCAG AGTCACCCGC CCATCATTGC GACCACCCCC 
CAGCCTGTGA AGACAAAGAA AGGGGTGAAG AGGAAAGCAG ATACCACCAC CCCTACCACC 
ATCGACCCCA TTCATGAGCC ACCCTCACTG GCCCCAGAGC CCAAGACCGC CAAGCTGGGT 
CCTCGGCGGG AGAGCAGCAG ACCTGTGAAG CCTCCAAAGA AGGATGTACC GGACTCACAG 
CAGCACCCAG GGCCAGAGAA GAGCAGCAAG ATCTCTGAGC AGCTAAAGTG CTGCAGTGGC 
ATCCTCAAGG AGATGTTTGC CAAGAAACAT GCTGCCTATG CCTGGCCTTT CTACAAGCCT 
GTGGATGTGG AGGCACTGGG TCTGCACGAC TACTGTGACA TCATCAAACA TCCCATGGAC 
ATGAGCACAA TCAAGTCTAA ACTAGAGTCC CGAGAGTACA GAGATGCCCA GGAATTTGGT 
GCTGATGTCC GATTGATGTT CTCCAACTGC TACAAGTACA ACCCCCCTGA CCATGAAGTG 
GTAGCCATGG CTCGAAAACT CCAGGATGTG TTTGAAATGC GCTTTGCCAA GATGCCTGAT 
GAGCCTGAAG AGCCAGTTGT TACAGTGTCC TCTCCTGCAG TGCCACCCCC TACAAAGGTG 
GTAGCCCCAC CCTCATCTAG TGACAGCAGC AGCGACAGTT CTTCCGACAG CGACAGTTCC 
ACTGACGACT CTGAGGAAGA GCGAGCCCAG CGGCTGGCTG AACTCCAGGA ACAGCTCAAG 
GCCGTGCATG AGCAGCTTGC AGCCCTCTCA CAGCCCCAGC AGAACAAACC AAAGAAAAAG 
GAGAAGGACA AGAAGGAAAA GAAAAAGGAA AAGCACAAAA AGAAAGAAGA AGTGGAGGAA 
AATAAAAAAA GCAAAACCAA GGAACTTCCT CCCAAAAAGA CAAAGAAAAA TAACAGCAGC 
AACAGCAATG TGAGCAAGAA GGAACCAGTA CCCACGAAGA CCAAGCCGCC TCCCACATAT 
GAATCAGAAG AGGAGGATAA GTGTAAGCCC ATGTCTTATG AGGAGAAGCG GCAGCTAAGT 
CTAGATATCA ACAAACTTCC TGGTGAGAAG CTAGGCCGTG TAGTACACAT AATTCAGTCA 
AGGGAACCAT CACTTAAAAA CTCCAACCCC GATGAGATTG AGATTGACTT TGAGACCCTG 
AAGCCATCTA CACTACGAGA GTTGGAGCGA TATGTCACCT CCTGTTTGCG GAAGAAAAGG 
AAACCTCAAG CTGAAAAAGT TGACGTGATT GCTGGTTCTT CCAAGATGAA GGGATTCTCA 
TCCTCTGAGT CGGAGAGCAC CAGCGAATCC AGCTCCTCTG ACAGTGAAGA CTCTGAAACA 
GAGATGGCTC CCAAGTCAAA AAAGAAGGGG CACACTGGGA GGGACCAGAA AAAGCATCAT 
CACCATCACC ATCCACAGAT GCAGCCAGCC CCAGCTCCTG TGCCCCAGCA GCCGCCCCCA 
CCTCCACAGC AGCCTCCTCC ACCCCCACCT CCGCAGCAGC AGCAGCAGCA ACCTCCACCC 
CCACCACCTC CGCCCTCCAT GCCACAGCAG ACTGCCCCAG CGATGAAGTC CTCGCCCCCA 
CCCTTCATCA CTGCCCAGGT CCCCGTCCTG GAACCACAGC TGCCAGGCAG TGTCTTTGAC 
CCTATTGGCC ACTTCACCCA GCCCATCTTG CACCTGCCGC AGCCGGAGCT GCCTCCTCAC 
CTGCCCCAGC CACCTGAGCA CAGCACTCCA CCCCATCTCA ACCAGCATGC TGTGGTCTCT 
CCTCCAGCTT TGCACAATGC GCTGCCCCAA CAGCCATCTC GGCCCAGTAA CCGAGCTGCT 
GCTCTGCCCC CAAAGCCTAC CCGACCCCCA GCTGTGTCCC CTGCCCTGGC CCAGCCCCCC 
CTGCTCCCAC AACCACCAAT GGCTCAGCCC CCCCAAGTGC TGCTGGAGGA TGAAGAGCCA 
CCTGCCCCAC CCCTCACCTC CATGCAGATG CAGTTGTACC TACAGCAGCT GCAGAAGGTG 
CAGCCTCCCA CACCACTACT CCCTTCCGTG AAGGTGCAGT CCCAACCCCC ACCGCCTTTG 
CCGCCTCCAC CCCACCCTTC TGTGCAGCAG CAGCAGCTCC AGCCACAGCC ACCGCCACCC 
CCACCTCCTC AGCCACAGCC ACCACCCCAG CAACAACACC AGCCTCCACC ACGACCAGTT 
CACTTGCCAT CCATGCCCTT TTCTGCTCAT ATTCAGCAGC CCCCACCACC CCCAGGACAG 
CAACCTACTC ACCCACCCCC AGGACAGCAG CCCCCACCAC CACAGCCTGC CAAGCCCCAG 
CAAGTCATCC AGCATCACCC TTCCCCCCGG CACCACAAGT CAGACCCCTA CTCAGCTGGT 
CATCTTCGTG AGGCTCCCTC TCCGCTTATG ATACATTCCC CTCAGATGCC ACAGTTCCAG 
AGCCTGACCC ATCAGTCTCC TCCCCAGCAA AACGTCCAGC CTAAGAAGCA GGTAAAGGGC 
AGGGCTGAGC CACAGCCACC AGGGCCAGTC ATGGGCCAAG GCCAGGGATG CCCACCTGCC 
TCACCGGCTG CCGTGCCTAT GCTGTCCCAG GAGCTGCGAC CACCTTCAGT CGTCCAGCCC 
CAGCCCCTGG TGGTAGTAAA GGAGGAGAAG ATTCACTCAC CAATCATTCG CAGCGAGCCT 
TTCAGCACCT CACTTCGACC AGAGCCCCCC AAGCACCCAG AGAACATCAA GGCCCCAGTC 
CACCTGCCCC AGCGGCCTGA GATGAAGCCT GTAGACATAG GGAGGCCTGT GATCCGGCCT 
CCAGAGCAGA GTGCACCCCC ACCAGGGGCC CCTGACAAGG ACAAACAGAA GCAGGAGCCA 
AAAACACCAG TGGCACCCAA AAAGGACCTG AAAATTAAGA ACATGGGCTC CTGGGCCAGC 
CTGGTACAGA AGCATCCGAC CACCCCATCC TCCACAGCCA AGTCCTCAAG TGACAGCTTT 
GAGCATTTCC GCCGTGCTGC TCGGGAGAAG GAGGAGAGGG AAAAGGCCCT GAAGGCTCAG 
GCTGAGCATG CAGAGAAGGA GAAGGAGCGG CTGAGGCAGG AGCGCATGAG GAGCCGAGAG 
GATGAAGATG CGCTGGAACA GGCACGTCGA GCCCATGAGG AGGCACGACG GCGCCAGGAG 
CAGCAGCAGC AGCAGCAGCA GCAGAGACAG GAGCAGCAGC AGCAGCAGCA GCAGGCAGCT 
GCTGTGGCCG CTGCCTCTGC CCCCCAGGCC CAGAGCTCCC AGCCCCAGTC TATGCTGGAC 
CAGCAGAGGG AGTTGGCCCG GAAACGAGAG CAGGAGCGGA GGCGCAGGGA GGCAATGGCA 
GCTACAATTG ACATGAATTT CCAGAGTGAT CTTTTGTCAA TATTTGAAGA AAATCTTTTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brd4 ( XM_006524688.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524751.1 Mus musculus bromodomain containing 4 (Brd4), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OMu16383
Clone ID Related Accession (Same CDS sequence)	XM_030249927.1 , XM_030249925.1 , XM_006524689.2 , NM_020508.4 , XM_006524688.1 , XM_006524688.2 , XM_030249926.1 , XM_006524689.3 , XM_030249924.1
Accession Version	XM_006524688.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4203bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	bromodomain-containing protein 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006524688.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGCC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGACAGACA 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCCGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAAAAA 
ATCAATGAAC TGCCTACAGA AGAAACTGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGAGGGAGGA AAGAAACAGG GGCAGCAAAG CCTGGTGTAT CCACGGTACC AAACACAACT 
CAAGCATCAA CTTCTCCGCA GACCCAGACG CCTCAGCAGA ACCCTCCTCC ACCTGTGCAG 
GCCACAACTC ACCCCTTTCC TGCTGTCACC CCAGACCTCA TTGCCCAGCC TCCTGTCATG 
ACAATGGTGC CCCCTCAGCC ACTTCAGACT CCTTCACCGG TACCCCCCCA GCCACCACCC 
CCACCTGCTC CAGTTCCACA GCCTGTGCAG AGTCACCCGC CCATCATTGC GACCACCCCC 
CAGCCTGTGA AGACAAAGAA AGGGGTGAAG AGGAAAGCAG ATACCACCAC CCCTACCACC 
ATCGACCCCA TTCATGAGCC ACCCTCACTG GCCCCAGAGC CCAAGACCGC CAAGCTGGGT 
CCTCGGCGGG AGAGCAGCAG ACCTGTGAAG CCTCCAAAGA AGGATGTACC GGACTCACAG 
CAGCACCCAG GGCCAGAGAA GAGCAGCAAG ATCTCTGAGC AGCTAAAGTG CTGCAGTGGC 
ATCCTCAAGG AGATGTTTGC CAAGAAACAT GCTGCCTATG CCTGGCCTTT CTACAAGCCT 
GTGGATGTGG AGGCACTGGG TCTGCACGAC TACTGTGACA TCATCAAACA TCCCATGGAC 
ATGAGCACAA TCAAGTCTAA ACTAGAGTCC CGAGAGTACA GAGATGCCCA GGAATTTGGT 
GCTGATGTCC GATTGATGTT CTCCAACTGC TACAAGTACA ACCCCCCTGA CCATGAAGTG 
GTAGCCATGG CTCGAAAACT CCAGGATGTG TTTGAAATGC GCTTTGCCAA GATGCCTGAT 
GAGCCTGAAG AGCCAGTTGT TACAGTGTCC TCTCCTGCAG TGCCACCCCC TACAAAGGTG 
GTAGCCCCAC CCTCATCTAG TGACAGCAGC AGCGACAGTT CTTCCGACAG CGACAGTTCC 
ACTGACGACT CTGAGGAAGA GCGAGCCCAG CGGCTGGCTG AACTCCAGGA ACAGCTCAAG 
GCCGTGCATG AGCAGCTTGC AGCCCTCTCA CAGCCCCAGC AGAACAAACC AAAGAAAAAG 
GAGAAGGACA AGAAGGAAAA GAAAAAGGAA AAGCACAAAA AGAAAGAAGA AGTGGAGGAA 
AATAAAAAAA GCAAAACCAA GGAACTTCCT CCCAAAAAGA CAAAGAAAAA TAACAGCAGC 
AACAGCAATG TGAGCAAGAA GGAACCAGTA CCCACGAAGA CCAAGCCGCC TCCCACATAT 
GAATCAGAAG AGGAGGATAA GTGTAAGCCC ATGTCTTATG AGGAGAAGCG GCAGCTAAGT 
CTAGATATCA ACAAACTTCC TGGTGAGAAG CTAGGCCGTG TAGTACACAT AATTCAGTCA 
AGGGAACCAT CACTTAAAAA CTCCAACCCC GATGAGATTG AGATTGACTT TGAGACCCTG 
AAGCCATCTA CACTACGAGA GTTGGAGCGA TATGTCACCT CCTGTTTGCG GAAGAAAAGG 
AAACCTCAAG CTGAAAAAGT TGACGTGATT GCTGGTTCTT CCAAGATGAA GGGATTCTCA 
TCCTCTGAGT CGGAGAGCAC CAGCGAATCC AGCTCCTCTG ACAGTGAAGA CTCTGAAACA 
GAGATGGCTC CCAAGTCAAA AAAGAAGGGG CACACTGGGA GGGACCAGAA AAAGCATCAT 
CACCATCACC ATCCACAGAT GCAGCCAGCC CCAGCTCCTG TGCCCCAGCA GCCGCCCCCA 
CCTCCACAGC AGCCTCCTCC ACCCCCACCT CCGCAGCAGC AGCAGCAGCA ACCTCCACCC 
CCACCACCTC CGCCCTCCAT GCCACAGCAG ACTGCCCCAG CGATGAAGTC CTCGCCCCCA 
CCCTTCATCA CTGCCCAGGT CCCCGTCCTG GAACCACAGC TGCCAGGCAG TGTCTTTGAC 
CCTATTGGCC ACTTCACCCA GCCCATCTTG CACCTGCCGC AGCCGGAGCT GCCTCCTCAC 
CTGCCCCAGC CACCTGAGCA CAGCACTCCA CCCCATCTCA ACCAGCATGC TGTGGTCTCT 
CCTCCAGCTT TGCACAATGC GCTGCCCCAA CAGCCATCTC GGCCCAGTAA CCGAGCTGCT 
GCTCTGCCCC CAAAGCCTAC CCGACCCCCA GCTGTGTCCC CTGCCCTGGC CCAGCCCCCC 
CTGCTCCCAC AACCACCAAT GGCTCAGCCC CCCCAAGTGC TGCTGGAGGA TGAAGAGCCA 
CCTGCCCCAC CCCTCACCTC CATGCAGATG CAGTTGTACC TACAGCAGCT GCAGAAGGTG 
CAGCCTCCCA CACCACTACT CCCTTCCGTG AAGGTGCAGT CCCAACCCCC ACCGCCTTTG 
CCGCCTCCAC CCCACCCTTC TGTGCAGCAG CAGCAGCTCC AGCCACAGCC ACCGCCACCC 
CCACCTCCTC AGCCACAGCC ACCACCCCAG CAACAACACC AGCCTCCACC ACGACCAGTT 
CACTTGCCAT CCATGCCCTT TTCTGCTCAT ATTCAGCAGC CCCCACCACC CCCAGGACAG 
CAACCTACTC ACCCACCCCC AGGACAGCAG CCCCCACCAC CACAGCCTGC CAAGCCCCAG 
CAAGTCATCC AGCATCACCC TTCCCCCCGG CACCACAAGT CAGACCCCTA CTCAGCTGGT 
CATCTTCGTG AGGCTCCCTC TCCGCTTATG ATACATTCCC CTCAGATGCC ACAGTTCCAG 
AGCCTGACCC ATCAGTCTCC TCCCCAGCAA AACGTCCAGC CTAAGAAGCA GGTAAAGGGC 
AGGGCTGAGC CACAGCCACC AGGGCCAGTC ATGGGCCAAG GCCAGGGATG CCCACCTGCC 
TCACCGGCTG CCGTGCCTAT GCTGTCCCAG GAGCTGCGAC CACCTTCAGT CGTCCAGCCC 
CAGCCCCTGG TGGTAGTAAA GGAGGAGAAG ATTCACTCAC CAATCATTCG CAGCGAGCCT 
TTCAGCACCT CACTTCGACC AGAGCCCCCC AAGCACCCAG AGAACATCAA GGCCCCAGTC 
CACCTGCCCC AGCGGCCTGA GATGAAGCCT GTAGACATAG GGAGGCCTGT GATCCGGCCT 
CCAGAGCAGA GTGCACCCCC ACCAGGGGCC CCTGACAAGG ACAAACAGAA GCAGGAGCCA 
AAAACACCAG TGGCACCCAA AAAGGACCTG AAAATTAAGA ACATGGGCTC CTGGGCCAGC 
CTGGTACAGA AGCATCCGAC CACCCCATCC TCCACAGCCA AGTCCTCAAG TGACAGCTTT 
GAGCATTTCC GCCGTGCTGC TCGGGAGAAG GAGGAGAGGG AAAAGGCCCT GAAGGCTCAG 
GCTGAGCATG CAGAGAAGGA GAAGGAGCGG CTGAGGCAGG AGCGCATGAG GAGCCGAGAG 
GATGAAGATG CGCTGGAACA GGCACGTCGA GCCCATGAGG AGGCACGACG GCGCCAGGAG 
CAGCAGCAGC AGCAGCAGCA GCAGAGACAG GAGCAGCAGC AGCAGCAGCA GCAGGCAGCT 
GCTGTGGCCG CTGCCTCTGC CCCCCAGGCC CAGAGCTCCC AGCCCCAGTC TATGCTGGAC 
CAGCAGAGGG AGTTGGCCCG GAAACGAGAG CAGGAGCGGA GGCGCAGGGA GGCAATGGCA 
GCTACAATTG ACATGAATTT CCAGAGTGAT CTTTTGTCAA TATTTGAAGA AAATCTTTTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524751.1
CDS	261..4463
Translation	MSTESGPGTRLRNLPVMGDGLETSQMSTTQAQAQPQPANAASTNPPPPETSNPNKPKRQTNQLQYLLRVVLKTLWKHQFAWPFQQPVDAVKLNLPDYYKIIKTPMDMGTIKKRLENNYYWNAQECIQDFNTMFTNCYIYNKPGDDIVLMAEALEKLFLQKINELPTEETEIMIVQAKGRGRGRKETGAAKPGVSTVPNTTQASTSPQTQTPQQNPPPPVQATTHPFPAVTPDLIAQPPVMTMVPPQPLQTPSPVPPQPPPPPAPVPQPVQSHPPIIATTPQPVKTKKGVKRKADTTTPTTIDPIHEPPSLAPEPKTAKLGPRRESSRPVKPPKKDVPDSQQHPGPEKSSKISEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYCDIIKHPMDMSTIKSKLESREYRDAQEFGADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPEEPVVTVSSPAVPPPTKVVAPPSSSDSSSDSSSDSDSSTDDSEEERAQRLAELQEQLKAVHEQLAALSQPQQNKPKKKEKDKKEKKKEKHKKKEEVEENKKSKTKELPPKKTKKNNSSNSNVSKKEPVPTKTKPPPTYESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVDVIAGSSKMKGFSSSESESTSESSSSDSEDSETEMAPKSKKKGHTGRDQKKHHHHHHPQMQPAPAPVPQQPPPPPQQPPPPPPPQQQQQQPPPPPPPPSMPQQTAPAMKSSPPPFITAQVPVLEPQLPGSVFDPIGHFTQPILHLPQPELPPHLPQPPEHSTPPHLNQHAVVSPPALHNALPQQPSRPSNRAAALPPKPTRPPAVSPALAQPPLLPQPPMAQPPQVLLEDEEPPAPPLTSMQMQLYLQQLQKVQPPTPLLPSVKVQSQPPPPLPPPPHPSVQQQQLQPQPPPPPPPQPQPPPQQQHQPPPRPVHLPSMPFSAHIQQPPPPPGQQPTHPPPGQQPPPPQPAKPQQVIQHHPSPRHHKSDPYSAGHLREAPSPLMIHSPQMPQFQSLTHQSPPQQNVQPKKQVKGRAEPQPPGPVMGQGQGCPPASPAAVPMLSQELRPPSVVQPQPLVVVKEEKIHSPIIRSEPFSTSLRPEPPKHPENIKAPVHLPQRPEMKPVDIGRPVIRPPEQSAPPPGAPDKDKQKQEPKTPVAPKKDLKIKNMGSWASLVQKHPTTPSSTAKSSSDSFEHFRRAAREKEEREKALKAQAEHAEKEKERLRQERMRSREDEDALEQARRAHEEARRRQEQQQQQQQQRQEQQQQQQQAAAVAAASAPQAQSSQPQSMLDQQRELARKREQERRRREAMAATIDMNFQSDLLSIFEENLF

Target ORF information:

RefSeq Version	XM_006524688.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus bromodomain containing 4 (Brd4), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524688.2

ORF Insert Sequence:

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGCC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGACAGACA 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCCGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAAAAA 
ATCAATGAAC TGCCTACAGA AGAAACTGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGAGGGAGGA AAGAAACAGG GGCAGCAAAG CCTGGTGTAT CCACGGTACC AAACACAACT 
CAAGCATCAA CTTCTCCGCA GACCCAGACG CCTCAGCAGA ACCCTCCTCC ACCTGTGCAG 
GCCACAACTC ACCCCTTTCC TGCTGTCACC CCAGACCTCA TTGCCCAGCC TCCTGTCATG 
ACAATGGTGC CCCCTCAGCC ACTTCAGACT CCTTCACCGG TACCCCCCCA GCCACCACCC 
CCACCTGCTC CAGTTCCACA GCCTGTGCAG AGTCACCCGC CCATCATTGC GACCACCCCC 
CAGCCTGTGA AGACAAAGAA AGGGGTGAAG AGGAAAGCAG ATACCACCAC CCCTACCACC 
ATCGACCCCA TTCATGAGCC ACCCTCACTG GCCCCAGAGC CCAAGACCGC CAAGCTGGGT 
CCTCGGCGGG AGAGCAGCAG ACCTGTGAAG CCTCCAAAGA AGGATGTACC GGACTCACAG 
CAGCACCCAG GGCCAGAGAA GAGCAGCAAG ATCTCTGAGC AGCTAAAGTG CTGCAGTGGC 
ATCCTCAAGG AGATGTTTGC CAAGAAACAT GCTGCCTATG CCTGGCCTTT CTACAAGCCT 
GTGGATGTGG AGGCACTGGG TCTGCACGAC TACTGTGACA TCATCAAACA TCCCATGGAC 
ATGAGCACAA TCAAGTCTAA ACTAGAGTCC CGAGAGTACA GAGATGCCCA GGAATTTGGT 
GCTGATGTCC GATTGATGTT CTCCAACTGC TACAAGTACA ACCCCCCTGA CCATGAAGTG 
GTAGCCATGG CTCGAAAACT CCAGGATGTG TTTGAAATGC GCTTTGCCAA GATGCCTGAT 
GAGCCTGAAG AGCCAGTTGT TACAGTGTCC TCTCCTGCAG TGCCACCCCC TACAAAGGTG 
GTAGCCCCAC CCTCATCTAG TGACAGCAGC AGCGACAGTT CTTCCGACAG CGACAGTTCC 
ACTGACGACT CTGAGGAAGA GCGAGCCCAG CGGCTGGCTG AACTCCAGGA ACAGCTCAAG 
GCCGTGCATG AGCAGCTTGC AGCCCTCTCA CAGCCCCAGC AGAACAAACC AAAGAAAAAG 
GAGAAGGACA AGAAGGAAAA GAAAAAGGAA AAGCACAAAA AGAAAGAAGA AGTGGAGGAA 
AATAAAAAAA GCAAAACCAA GGAACTTCCT CCCAAAAAGA CAAAGAAAAA TAACAGCAGC 
AACAGCAATG TGAGCAAGAA GGAACCAGTA CCCACGAAGA CCAAGCCGCC TCCCACATAT 
GAATCAGAAG AGGAGGATAA GTGTAAGCCC ATGTCTTATG AGGAGAAGCG GCAGCTAAGT 
CTAGATATCA ACAAACTTCC TGGTGAGAAG CTAGGCCGTG TAGTACACAT AATTCAGTCA 
AGGGAACCAT CACTTAAAAA CTCCAACCCC GATGAGATTG AGATTGACTT TGAGACCCTG 
AAGCCATCTA CACTACGAGA GTTGGAGCGA TATGTCACCT CCTGTTTGCG GAAGAAAAGG 
AAACCTCAAG CTGAAAAAGT TGACGTGATT GCTGGTTCTT CCAAGATGAA GGGATTCTCA 
TCCTCTGAGT CGGAGAGCAC CAGCGAATCC AGCTCCTCTG ACAGTGAAGA CTCTGAAACA 
GAGATGGCTC CCAAGTCAAA AAAGAAGGGG CACACTGGGA GGGACCAGAA AAAGCATCAT 
CACCATCACC ATCCACAGAT GCAGCCAGCC CCAGCTCCTG TGCCCCAGCA GCCGCCCCCA 
CCTCCACAGC AGCCTCCTCC ACCCCCACCT CCGCAGCAGC AGCAGCAGCA ACCTCCACCC 
CCACCACCTC CGCCCTCCAT GCCACAGCAG ACTGCCCCAG CGATGAAGTC CTCGCCCCCA 
CCCTTCATCA CTGCCCAGGT CCCCGTCCTG GAACCACAGC TGCCAGGCAG TGTCTTTGAC 
CCTATTGGCC ACTTCACCCA GCCCATCTTG CACCTGCCGC AGCCGGAGCT GCCTCCTCAC 
CTGCCCCAGC CACCTGAGCA CAGCACTCCA CCCCATCTCA ACCAGCATGC TGTGGTCTCT 
CCTCCAGCTT TGCACAATGC GCTGCCCCAA CAGCCATCTC GGCCCAGTAA CCGAGCTGCT 
GCTCTGCCCC CAAAGCCTAC CCGACCCCCA GCTGTGTCCC CTGCCCTGGC CCAGCCCCCC 
CTGCTCCCAC AACCACCAAT GGCTCAGCCC CCCCAAGTGC TGCTGGAGGA TGAAGAGCCA 
CCTGCCCCAC CCCTCACCTC CATGCAGATG CAGTTGTACC TACAGCAGCT GCAGAAGGTG 
CAGCCTCCCA CACCACTACT CCCTTCCGTG AAGGTGCAGT CCCAACCCCC ACCGCCTTTG 
CCGCCTCCAC CCCACCCTTC TGTGCAGCAG CAGCAGCTCC AGCCACAGCC ACCGCCACCC 
CCACCTCCTC AGCCACAGCC ACCACCCCAG CAACAACACC AGCCTCCACC ACGACCAGTT 
CACTTGCCAT CCATGCCCTT TTCTGCTCAT ATTCAGCAGC CCCCACCACC CCCAGGACAG 
CAACCTACTC ACCCACCCCC AGGACAGCAG CCCCCACCAC CACAGCCTGC CAAGCCCCAG 
CAAGTCATCC AGCATCACCC TTCCCCCCGG CACCACAAGT CAGACCCCTA CTCAGCTGGT 
CATCTTCGTG AGGCTCCCTC TCCGCTTATG ATACATTCCC CTCAGATGCC ACAGTTCCAG 
AGCCTGACCC ATCAGTCTCC TCCCCAGCAA AACGTCCAGC CTAAGAAGCA GGTAAAGGGC 
AGGGCTGAGC CACAGCCACC AGGGCCAGTC ATGGGCCAAG GCCAGGGATG CCCACCTGCC 
TCACCGGCTG CCGTGCCTAT GCTGTCCCAG GAGCTGCGAC CACCTTCAGT CGTCCAGCCC 
CAGCCCCTGG TGGTAGTAAA GGAGGAGAAG ATTCACTCAC CAATCATTCG CAGCGAGCCT 
TTCAGCACCT CACTTCGACC AGAGCCCCCC AAGCACCCAG AGAACATCAA GGCCCCAGTC 
CACCTGCCCC AGCGGCCTGA GATGAAGCCT GTAGACATAG GGAGGCCTGT GATCCGGCCT 
CCAGAGCAGA GTGCACCCCC ACCAGGGGCC CCTGACAAGG ACAAACAGAA GCAGGAGCCA 
AAAACACCAG TGGCACCCAA AAAGGACCTG AAAATTAAGA ACATGGGCTC CTGGGCCAGC 
CTGGTACAGA AGCATCCGAC CACCCCATCC TCCACAGCCA AGTCCTCAAG TGACAGCTTT 
GAGCATTTCC GCCGTGCTGC TCGGGAGAAG GAGGAGAGGG AAAAGGCCCT GAAGGCTCAG 
GCTGAGCATG CAGAGAAGGA GAAGGAGCGG CTGAGGCAGG AGCGCATGAG GAGCCGAGAG 
GATGAAGATG CGCTGGAACA GGCACGTCGA GCCCATGAGG AGGCACGACG GCGCCAGGAG 
CAGCAGCAGC AGCAGCAGCA GCAGAGACAG GAGCAGCAGC AGCAGCAGCA GCAGGCAGCT 
GCTGTGGCCG CTGCCTCTGC CCCCCAGGCC CAGAGCTCCC AGCCCCAGTC TATGCTGGAC 
CAGCAGAGGG AGTTGGCCCG GAAACGAGAG CAGGAGCGGA GGCGCAGGGA GGCAATGGCA 
GCTACAATTG ACATGAATTT CCAGAGTGAT CTTTTGTCAA TATTTGAAGA AAATCTTTTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brd4 ( XM_030249926.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105786.1 Mus musculus bromodomain containing 4 (Brd4), transcript variant X11, mRNA.

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CloneID	OMu16383
Clone ID Related Accession (Same CDS sequence)	XM_030249927.1 , XM_030249925.1 , XM_006524689.2 , NM_020508.4 , XM_006524688.1 , XM_006524688.2 , XM_030249926.1 , XM_006524689.3 , XM_030249924.1
Accession Version	XM_030249926.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4203bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	bromodomain-containing protein 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGCC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGACAGACA 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCCGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAAAAA 
ATCAATGAAC TGCCTACAGA AGAAACTGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGAGGGAGGA AAGAAACAGG GGCAGCAAAG CCTGGTGTAT CCACGGTACC AAACACAACT 
CAAGCATCAA CTTCTCCGCA GACCCAGACG CCTCAGCAGA ACCCTCCTCC ACCTGTGCAG 
GCCACAACTC ACCCCTTTCC TGCTGTCACC CCAGACCTCA TTGCCCAGCC TCCTGTCATG 
ACAATGGTGC CCCCTCAGCC ACTTCAGACT CCTTCACCGG TACCCCCCCA GCCACCACCC 
CCACCTGCTC CAGTTCCACA GCCTGTGCAG AGTCACCCGC CCATCATTGC GACCACCCCC 
CAGCCTGTGA AGACAAAGAA AGGGGTGAAG AGGAAAGCAG ATACCACCAC CCCTACCACC 
ATCGACCCCA TTCATGAGCC ACCCTCACTG GCCCCAGAGC CCAAGACCGC CAAGCTGGGT 
CCTCGGCGGG AGAGCAGCAG ACCTGTGAAG CCTCCAAAGA AGGATGTACC GGACTCACAG 
CAGCACCCAG GGCCAGAGAA GAGCAGCAAG ATCTCTGAGC AGCTAAAGTG CTGCAGTGGC 
ATCCTCAAGG AGATGTTTGC CAAGAAACAT GCTGCCTATG CCTGGCCTTT CTACAAGCCT 
GTGGATGTGG AGGCACTGGG TCTGCACGAC TACTGTGACA TCATCAAACA TCCCATGGAC 
ATGAGCACAA TCAAGTCTAA ACTAGAGTCC CGAGAGTACA GAGATGCCCA GGAATTTGGT 
GCTGATGTCC GATTGATGTT CTCCAACTGC TACAAGTACA ACCCCCCTGA CCATGAAGTG 
GTAGCCATGG CTCGAAAACT CCAGGATGTG TTTGAAATGC GCTTTGCCAA GATGCCTGAT 
GAGCCTGAAG AGCCAGTTGT TACAGTGTCC TCTCCTGCAG TGCCACCCCC TACAAAGGTG 
GTAGCCCCAC CCTCATCTAG TGACAGCAGC AGCGACAGTT CTTCCGACAG CGACAGTTCC 
ACTGACGACT CTGAGGAAGA GCGAGCCCAG CGGCTGGCTG AACTCCAGGA ACAGCTCAAG 
GCCGTGCATG AGCAGCTTGC AGCCCTCTCA CAGCCCCAGC AGAACAAACC AAAGAAAAAG 
GAGAAGGACA AGAAGGAAAA GAAAAAGGAA AAGCACAAAA AGAAAGAAGA AGTGGAGGAA 
AATAAAAAAA GCAAAACCAA GGAACTTCCT CCCAAAAAGA CAAAGAAAAA TAACAGCAGC 
AACAGCAATG TGAGCAAGAA GGAACCAGTA CCCACGAAGA CCAAGCCGCC TCCCACATAT 
GAATCAGAAG AGGAGGATAA GTGTAAGCCC ATGTCTTATG AGGAGAAGCG GCAGCTAAGT 
CTAGATATCA ACAAACTTCC TGGTGAGAAG CTAGGCCGTG TAGTACACAT AATTCAGTCA 
AGGGAACCAT CACTTAAAAA CTCCAACCCC GATGAGATTG AGATTGACTT TGAGACCCTG 
AAGCCATCTA CACTACGAGA GTTGGAGCGA TATGTCACCT CCTGTTTGCG GAAGAAAAGG 
AAACCTCAAG CTGAAAAAGT TGACGTGATT GCTGGTTCTT CCAAGATGAA GGGATTCTCA 
TCCTCTGAGT CGGAGAGCAC CAGCGAATCC AGCTCCTCTG ACAGTGAAGA CTCTGAAACA 
GAGATGGCTC CCAAGTCAAA AAAGAAGGGG CACACTGGGA GGGACCAGAA AAAGCATCAT 
CACCATCACC ATCCACAGAT GCAGCCAGCC CCAGCTCCTG TGCCCCAGCA GCCGCCCCCA 
CCTCCACAGC AGCCTCCTCC ACCCCCACCT CCGCAGCAGC AGCAGCAGCA ACCTCCACCC 
CCACCACCTC CGCCCTCCAT GCCACAGCAG ACTGCCCCAG CGATGAAGTC CTCGCCCCCA 
CCCTTCATCA CTGCCCAGGT CCCCGTCCTG GAACCACAGC TGCCAGGCAG TGTCTTTGAC 
CCTATTGGCC ACTTCACCCA GCCCATCTTG CACCTGCCGC AGCCGGAGCT GCCTCCTCAC 
CTGCCCCAGC CACCTGAGCA CAGCACTCCA CCCCATCTCA ACCAGCATGC TGTGGTCTCT 
CCTCCAGCTT TGCACAATGC GCTGCCCCAA CAGCCATCTC GGCCCAGTAA CCGAGCTGCT 
GCTCTGCCCC CAAAGCCTAC CCGACCCCCA GCTGTGTCCC CTGCCCTGGC CCAGCCCCCC 
CTGCTCCCAC AACCACCAAT GGCTCAGCCC CCCCAAGTGC TGCTGGAGGA TGAAGAGCCA 
CCTGCCCCAC CCCTCACCTC CATGCAGATG CAGTTGTACC TACAGCAGCT GCAGAAGGTG 
CAGCCTCCCA CACCACTACT CCCTTCCGTG AAGGTGCAGT CCCAACCCCC ACCGCCTTTG 
CCGCCTCCAC CCCACCCTTC TGTGCAGCAG CAGCAGCTCC AGCCACAGCC ACCGCCACCC 
CCACCTCCTC AGCCACAGCC ACCACCCCAG CAACAACACC AGCCTCCACC ACGACCAGTT 
CACTTGCCAT CCATGCCCTT TTCTGCTCAT ATTCAGCAGC CCCCACCACC CCCAGGACAG 
CAACCTACTC ACCCACCCCC AGGACAGCAG CCCCCACCAC CACAGCCTGC CAAGCCCCAG 
CAAGTCATCC AGCATCACCC TTCCCCCCGG CACCACAAGT CAGACCCCTA CTCAGCTGGT 
CATCTTCGTG AGGCTCCCTC TCCGCTTATG ATACATTCCC CTCAGATGCC ACAGTTCCAG 
AGCCTGACCC ATCAGTCTCC TCCCCAGCAA AACGTCCAGC CTAAGAAGCA GGTAAAGGGC 
AGGGCTGAGC CACAGCCACC AGGGCCAGTC ATGGGCCAAG GCCAGGGATG CCCACCTGCC 
TCACCGGCTG CCGTGCCTAT GCTGTCCCAG GAGCTGCGAC CACCTTCAGT CGTCCAGCCC 
CAGCCCCTGG TGGTAGTAAA GGAGGAGAAG ATTCACTCAC CAATCATTCG CAGCGAGCCT 
TTCAGCACCT CACTTCGACC AGAGCCCCCC AAGCACCCAG AGAACATCAA GGCCCCAGTC 
CACCTGCCCC AGCGGCCTGA GATGAAGCCT GTAGACATAG GGAGGCCTGT GATCCGGCCT 
CCAGAGCAGA GTGCACCCCC ACCAGGGGCC CCTGACAAGG ACAAACAGAA GCAGGAGCCA 
AAAACACCAG TGGCACCCAA AAAGGACCTG AAAATTAAGA ACATGGGCTC CTGGGCCAGC 
CTGGTACAGA AGCATCCGAC CACCCCATCC TCCACAGCCA AGTCCTCAAG TGACAGCTTT 
GAGCATTTCC GCCGTGCTGC TCGGGAGAAG GAGGAGAGGG AAAAGGCCCT GAAGGCTCAG 
GCTGAGCATG CAGAGAAGGA GAAGGAGCGG CTGAGGCAGG AGCGCATGAG GAGCCGAGAG 
GATGAAGATG CGCTGGAACA GGCACGTCGA GCCCATGAGG AGGCACGACG GCGCCAGGAG 
CAGCAGCAGC AGCAGCAGCA GCAGAGACAG GAGCAGCAGC AGCAGCAGCA GCAGGCAGCT 
GCTGTGGCCG CTGCCTCTGC CCCCCAGGCC CAGAGCTCCC AGCCCCAGTC TATGCTGGAC 
CAGCAGAGGG AGTTGGCCCG GAAACGAGAG CAGGAGCGGA GGCGCAGGGA GGCAATGGCA 
GCTACAATTG ACATGAATTT CCAGAGTGAT CTTTTGTCAA TATTTGAAGA AAATCTTTTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105786.1
CDS	457..4659
Translation	MSTESGPGTRLRNLPVMGDGLETSQMSTTQAQAQPQPANAASTNPPPPETSNPNKPKRQTNQLQYLLRVVLKTLWKHQFAWPFQQPVDAVKLNLPDYYKIIKTPMDMGTIKKRLENNYYWNAQECIQDFNTMFTNCYIYNKPGDDIVLMAEALEKLFLQKINELPTEETEIMIVQAKGRGRGRKETGAAKPGVSTVPNTTQASTSPQTQTPQQNPPPPVQATTHPFPAVTPDLIAQPPVMTMVPPQPLQTPSPVPPQPPPPPAPVPQPVQSHPPIIATTPQPVKTKKGVKRKADTTTPTTIDPIHEPPSLAPEPKTAKLGPRRESSRPVKPPKKDVPDSQQHPGPEKSSKISEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYCDIIKHPMDMSTIKSKLESREYRDAQEFGADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPEEPVVTVSSPAVPPPTKVVAPPSSSDSSSDSSSDSDSSTDDSEEERAQRLAELQEQLKAVHEQLAALSQPQQNKPKKKEKDKKEKKKEKHKKKEEVEENKKSKTKELPPKKTKKNNSSNSNVSKKEPVPTKTKPPPTYESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVDVIAGSSKMKGFSSSESESTSESSSSDSEDSETEMAPKSKKKGHTGRDQKKHHHHHHPQMQPAPAPVPQQPPPPPQQPPPPPPPQQQQQQPPPPPPPPSMPQQTAPAMKSSPPPFITAQVPVLEPQLPGSVFDPIGHFTQPILHLPQPELPPHLPQPPEHSTPPHLNQHAVVSPPALHNALPQQPSRPSNRAAALPPKPTRPPAVSPALAQPPLLPQPPMAQPPQVLLEDEEPPAPPLTSMQMQLYLQQLQKVQPPTPLLPSVKVQSQPPPPLPPPPHPSVQQQQLQPQPPPPPPPQPQPPPQQQHQPPPRPVHLPSMPFSAHIQQPPPPPGQQPTHPPPGQQPPPPQPAKPQQVIQHHPSPRHHKSDPYSAGHLREAPSPLMIHSPQMPQFQSLTHQSPPQQNVQPKKQVKGRAEPQPPGPVMGQGQGCPPASPAAVPMLSQELRPPSVVQPQPLVVVKEEKIHSPIIRSEPFSTSLRPEPPKHPENIKAPVHLPQRPEMKPVDIGRPVIRPPEQSAPPPGAPDKDKQKQEPKTPVAPKKDLKIKNMGSWASLVQKHPTTPSSTAKSSSDSFEHFRRAAREKEEREKALKAQAEHAEKEKERLRQERMRSREDEDALEQARRAHEEARRRQEQQQQQQQQRQEQQQQQQQAAAVAAASAPQAQSSQPQSMLDQQRELARKREQERRRREAMAATIDMNFQSDLLSIFEENLF

Target ORF information:

RefSeq Version	XM_030249926.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus bromodomain containing 4 (Brd4), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249926.1

ORF Insert Sequence:

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGCC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGACAGACA 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCCGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAAAAA 
ATCAATGAAC TGCCTACAGA AGAAACTGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGAGGGAGGA AAGAAACAGG GGCAGCAAAG CCTGGTGTAT CCACGGTACC AAACACAACT 
CAAGCATCAA CTTCTCCGCA GACCCAGACG CCTCAGCAGA ACCCTCCTCC ACCTGTGCAG 
GCCACAACTC ACCCCTTTCC TGCTGTCACC CCAGACCTCA TTGCCCAGCC TCCTGTCATG 
ACAATGGTGC CCCCTCAGCC ACTTCAGACT CCTTCACCGG TACCCCCCCA GCCACCACCC 
CCACCTGCTC CAGTTCCACA GCCTGTGCAG AGTCACCCGC CCATCATTGC GACCACCCCC 
CAGCCTGTGA AGACAAAGAA AGGGGTGAAG AGGAAAGCAG ATACCACCAC CCCTACCACC 
ATCGACCCCA TTCATGAGCC ACCCTCACTG GCCCCAGAGC CCAAGACCGC CAAGCTGGGT 
CCTCGGCGGG AGAGCAGCAG ACCTGTGAAG CCTCCAAAGA AGGATGTACC GGACTCACAG 
CAGCACCCAG GGCCAGAGAA GAGCAGCAAG ATCTCTGAGC AGCTAAAGTG CTGCAGTGGC 
ATCCTCAAGG AGATGTTTGC CAAGAAACAT GCTGCCTATG CCTGGCCTTT CTACAAGCCT 
GTGGATGTGG AGGCACTGGG TCTGCACGAC TACTGTGACA TCATCAAACA TCCCATGGAC 
ATGAGCACAA TCAAGTCTAA ACTAGAGTCC CGAGAGTACA GAGATGCCCA GGAATTTGGT 
GCTGATGTCC GATTGATGTT CTCCAACTGC TACAAGTACA ACCCCCCTGA CCATGAAGTG 
GTAGCCATGG CTCGAAAACT CCAGGATGTG TTTGAAATGC GCTTTGCCAA GATGCCTGAT 
GAGCCTGAAG AGCCAGTTGT TACAGTGTCC TCTCCTGCAG TGCCACCCCC TACAAAGGTG 
GTAGCCCCAC CCTCATCTAG TGACAGCAGC AGCGACAGTT CTTCCGACAG CGACAGTTCC 
ACTGACGACT CTGAGGAAGA GCGAGCCCAG CGGCTGGCTG AACTCCAGGA ACAGCTCAAG 
GCCGTGCATG AGCAGCTTGC AGCCCTCTCA CAGCCCCAGC AGAACAAACC AAAGAAAAAG 
GAGAAGGACA AGAAGGAAAA GAAAAAGGAA AAGCACAAAA AGAAAGAAGA AGTGGAGGAA 
AATAAAAAAA GCAAAACCAA GGAACTTCCT CCCAAAAAGA CAAAGAAAAA TAACAGCAGC 
AACAGCAATG TGAGCAAGAA GGAACCAGTA CCCACGAAGA CCAAGCCGCC TCCCACATAT 
GAATCAGAAG AGGAGGATAA GTGTAAGCCC ATGTCTTATG AGGAGAAGCG GCAGCTAAGT 
CTAGATATCA ACAAACTTCC TGGTGAGAAG CTAGGCCGTG TAGTACACAT AATTCAGTCA 
AGGGAACCAT CACTTAAAAA CTCCAACCCC GATGAGATTG AGATTGACTT TGAGACCCTG 
AAGCCATCTA CACTACGAGA GTTGGAGCGA TATGTCACCT CCTGTTTGCG GAAGAAAAGG 
AAACCTCAAG CTGAAAAAGT TGACGTGATT GCTGGTTCTT CCAAGATGAA GGGATTCTCA 
TCCTCTGAGT CGGAGAGCAC CAGCGAATCC AGCTCCTCTG ACAGTGAAGA CTCTGAAACA 
GAGATGGCTC CCAAGTCAAA AAAGAAGGGG CACACTGGGA GGGACCAGAA AAAGCATCAT 
CACCATCACC ATCCACAGAT GCAGCCAGCC CCAGCTCCTG TGCCCCAGCA GCCGCCCCCA 
CCTCCACAGC AGCCTCCTCC ACCCCCACCT CCGCAGCAGC AGCAGCAGCA ACCTCCACCC 
CCACCACCTC CGCCCTCCAT GCCACAGCAG ACTGCCCCAG CGATGAAGTC CTCGCCCCCA 
CCCTTCATCA CTGCCCAGGT CCCCGTCCTG GAACCACAGC TGCCAGGCAG TGTCTTTGAC 
CCTATTGGCC ACTTCACCCA GCCCATCTTG CACCTGCCGC AGCCGGAGCT GCCTCCTCAC 
CTGCCCCAGC CACCTGAGCA CAGCACTCCA CCCCATCTCA ACCAGCATGC TGTGGTCTCT 
CCTCCAGCTT TGCACAATGC GCTGCCCCAA CAGCCATCTC GGCCCAGTAA CCGAGCTGCT 
GCTCTGCCCC CAAAGCCTAC CCGACCCCCA GCTGTGTCCC CTGCCCTGGC CCAGCCCCCC 
CTGCTCCCAC AACCACCAAT GGCTCAGCCC CCCCAAGTGC TGCTGGAGGA TGAAGAGCCA 
CCTGCCCCAC CCCTCACCTC CATGCAGATG CAGTTGTACC TACAGCAGCT GCAGAAGGTG 
CAGCCTCCCA CACCACTACT CCCTTCCGTG AAGGTGCAGT CCCAACCCCC ACCGCCTTTG 
CCGCCTCCAC CCCACCCTTC TGTGCAGCAG CAGCAGCTCC AGCCACAGCC ACCGCCACCC 
CCACCTCCTC AGCCACAGCC ACCACCCCAG CAACAACACC AGCCTCCACC ACGACCAGTT 
CACTTGCCAT CCATGCCCTT TTCTGCTCAT ATTCAGCAGC CCCCACCACC CCCAGGACAG 
CAACCTACTC ACCCACCCCC AGGACAGCAG CCCCCACCAC CACAGCCTGC CAAGCCCCAG 
CAAGTCATCC AGCATCACCC TTCCCCCCGG CACCACAAGT CAGACCCCTA CTCAGCTGGT 
CATCTTCGTG AGGCTCCCTC TCCGCTTATG ATACATTCCC CTCAGATGCC ACAGTTCCAG 
AGCCTGACCC ATCAGTCTCC TCCCCAGCAA AACGTCCAGC CTAAGAAGCA GGTAAAGGGC 
AGGGCTGAGC CACAGCCACC AGGGCCAGTC ATGGGCCAAG GCCAGGGATG CCCACCTGCC 
TCACCGGCTG CCGTGCCTAT GCTGTCCCAG GAGCTGCGAC CACCTTCAGT CGTCCAGCCC 
CAGCCCCTGG TGGTAGTAAA GGAGGAGAAG ATTCACTCAC CAATCATTCG CAGCGAGCCT 
TTCAGCACCT CACTTCGACC AGAGCCCCCC AAGCACCCAG AGAACATCAA GGCCCCAGTC 
CACCTGCCCC AGCGGCCTGA GATGAAGCCT GTAGACATAG GGAGGCCTGT GATCCGGCCT 
CCAGAGCAGA GTGCACCCCC ACCAGGGGCC CCTGACAAGG ACAAACAGAA GCAGGAGCCA 
AAAACACCAG TGGCACCCAA AAAGGACCTG AAAATTAAGA ACATGGGCTC CTGGGCCAGC 
CTGGTACAGA AGCATCCGAC CACCCCATCC TCCACAGCCA AGTCCTCAAG TGACAGCTTT 
GAGCATTTCC GCCGTGCTGC TCGGGAGAAG GAGGAGAGGG AAAAGGCCCT GAAGGCTCAG 
GCTGAGCATG CAGAGAAGGA GAAGGAGCGG CTGAGGCAGG AGCGCATGAG GAGCCGAGAG 
GATGAAGATG CGCTGGAACA GGCACGTCGA GCCCATGAGG AGGCACGACG GCGCCAGGAG 
CAGCAGCAGC AGCAGCAGCA GCAGAGACAG GAGCAGCAGC AGCAGCAGCA GCAGGCAGCT 
GCTGTGGCCG CTGCCTCTGC CCCCCAGGCC CAGAGCTCCC AGCCCCAGTC TATGCTGGAC 
CAGCAGAGGG AGTTGGCCCG GAAACGAGAG CAGGAGCGGA GGCGCAGGGA GGCAATGGCA 
GCTACAATTG ACATGAATTT CCAGAGTGAT CTTTTGTCAA TATTTGAAGA AAATCTTTTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brd4 ( XM_006524689.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524752.1 Mus musculus bromodomain containing 4 (Brd4), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OMu16383
Clone ID Related Accession (Same CDS sequence)	XM_030249927.1 , XM_030249925.1 , XM_006524689.2 , NM_020508.4 , XM_006524688.1 , XM_006524688.2 , XM_030249926.1 , XM_006524689.3 , XM_030249924.1
Accession Version	XM_006524689.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4203bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	bromodomain-containing protein 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006524689.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGCC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGACAGACA 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCCGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAAAAA 
ATCAATGAAC TGCCTACAGA AGAAACTGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGAGGGAGGA AAGAAACAGG GGCAGCAAAG CCTGGTGTAT CCACGGTACC AAACACAACT 
CAAGCATCAA CTTCTCCGCA GACCCAGACG CCTCAGCAGA ACCCTCCTCC ACCTGTGCAG 
GCCACAACTC ACCCCTTTCC TGCTGTCACC CCAGACCTCA TTGCCCAGCC TCCTGTCATG 
ACAATGGTGC CCCCTCAGCC ACTTCAGACT CCTTCACCGG TACCCCCCCA GCCACCACCC 
CCACCTGCTC CAGTTCCACA GCCTGTGCAG AGTCACCCGC CCATCATTGC GACCACCCCC 
CAGCCTGTGA AGACAAAGAA AGGGGTGAAG AGGAAAGCAG ATACCACCAC CCCTACCACC 
ATCGACCCCA TTCATGAGCC ACCCTCACTG GCCCCAGAGC CCAAGACCGC CAAGCTGGGT 
CCTCGGCGGG AGAGCAGCAG ACCTGTGAAG CCTCCAAAGA AGGATGTACC GGACTCACAG 
CAGCACCCAG GGCCAGAGAA GAGCAGCAAG ATCTCTGAGC AGCTAAAGTG CTGCAGTGGC 
ATCCTCAAGG AGATGTTTGC CAAGAAACAT GCTGCCTATG CCTGGCCTTT CTACAAGCCT 
GTGGATGTGG AGGCACTGGG TCTGCACGAC TACTGTGACA TCATCAAACA TCCCATGGAC 
ATGAGCACAA TCAAGTCTAA ACTAGAGTCC CGAGAGTACA GAGATGCCCA GGAATTTGGT 
GCTGATGTCC GATTGATGTT CTCCAACTGC TACAAGTACA ACCCCCCTGA CCATGAAGTG 
GTAGCCATGG CTCGAAAACT CCAGGATGTG TTTGAAATGC GCTTTGCCAA GATGCCTGAT 
GAGCCTGAAG AGCCAGTTGT TACAGTGTCC TCTCCTGCAG TGCCACCCCC TACAAAGGTG 
GTAGCCCCAC CCTCATCTAG TGACAGCAGC AGCGACAGTT CTTCCGACAG CGACAGTTCC 
ACTGACGACT CTGAGGAAGA GCGAGCCCAG CGGCTGGCTG AACTCCAGGA ACAGCTCAAG 
GCCGTGCATG AGCAGCTTGC AGCCCTCTCA CAGCCCCAGC AGAACAAACC AAAGAAAAAG 
GAGAAGGACA AGAAGGAAAA GAAAAAGGAA AAGCACAAAA AGAAAGAAGA AGTGGAGGAA 
AATAAAAAAA GCAAAACCAA GGAACTTCCT CCCAAAAAGA CAAAGAAAAA TAACAGCAGC 
AACAGCAATG TGAGCAAGAA GGAACCAGTA CCCACGAAGA CCAAGCCGCC TCCCACATAT 
GAATCAGAAG AGGAGGATAA GTGTAAGCCC ATGTCTTATG AGGAGAAGCG GCAGCTAAGT 
CTAGATATCA ACAAACTTCC TGGTGAGAAG CTAGGCCGTG TAGTACACAT AATTCAGTCA 
AGGGAACCAT CACTTAAAAA CTCCAACCCC GATGAGATTG AGATTGACTT TGAGACCCTG 
AAGCCATCTA CACTACGAGA GTTGGAGCGA TATGTCACCT CCTGTTTGCG GAAGAAAAGG 
AAACCTCAAG CTGAAAAAGT TGACGTGATT GCTGGTTCTT CCAAGATGAA GGGATTCTCA 
TCCTCTGAGT CGGAGAGCAC CAGCGAATCC AGCTCCTCTG ACAGTGAAGA CTCTGAAACA 
GAGATGGCTC CCAAGTCAAA AAAGAAGGGG CACACTGGGA GGGACCAGAA AAAGCATCAT 
CACCATCACC ATCCACAGAT GCAGCCAGCC CCAGCTCCTG TGCCCCAGCA GCCGCCCCCA 
CCTCCACAGC AGCCTCCTCC ACCCCCACCT CCGCAGCAGC AGCAGCAGCA ACCTCCACCC 
CCACCACCTC CGCCCTCCAT GCCACAGCAG ACTGCCCCAG CGATGAAGTC CTCGCCCCCA 
CCCTTCATCA CTGCCCAGGT CCCCGTCCTG GAACCACAGC TGCCAGGCAG TGTCTTTGAC 
CCTATTGGCC ACTTCACCCA GCCCATCTTG CACCTGCCGC AGCCGGAGCT GCCTCCTCAC 
CTGCCCCAGC CACCTGAGCA CAGCACTCCA CCCCATCTCA ACCAGCATGC TGTGGTCTCT 
CCTCCAGCTT TGCACAATGC GCTGCCCCAA CAGCCATCTC GGCCCAGTAA CCGAGCTGCT 
GCTCTGCCCC CAAAGCCTAC CCGACCCCCA GCTGTGTCCC CTGCCCTGGC CCAGCCCCCC 
CTGCTCCCAC AACCACCAAT GGCTCAGCCC CCCCAAGTGC TGCTGGAGGA TGAAGAGCCA 
CCTGCCCCAC CCCTCACCTC CATGCAGATG CAGTTGTACC TACAGCAGCT GCAGAAGGTG 
CAGCCTCCCA CACCACTACT CCCTTCCGTG AAGGTGCAGT CCCAACCCCC ACCGCCTTTG 
CCGCCTCCAC CCCACCCTTC TGTGCAGCAG CAGCAGCTCC AGCCACAGCC ACCGCCACCC 
CCACCTCCTC AGCCACAGCC ACCACCCCAG CAACAACACC AGCCTCCACC ACGACCAGTT 
CACTTGCCAT CCATGCCCTT TTCTGCTCAT ATTCAGCAGC CCCCACCACC CCCAGGACAG 
CAACCTACTC ACCCACCCCC AGGACAGCAG CCCCCACCAC CACAGCCTGC CAAGCCCCAG 
CAAGTCATCC AGCATCACCC TTCCCCCCGG CACCACAAGT CAGACCCCTA CTCAGCTGGT 
CATCTTCGTG AGGCTCCCTC TCCGCTTATG ATACATTCCC CTCAGATGCC ACAGTTCCAG 
AGCCTGACCC ATCAGTCTCC TCCCCAGCAA AACGTCCAGC CTAAGAAGCA GGTAAAGGGC 
AGGGCTGAGC CACAGCCACC AGGGCCAGTC ATGGGCCAAG GCCAGGGATG CCCACCTGCC 
TCACCGGCTG CCGTGCCTAT GCTGTCCCAG GAGCTGCGAC CACCTTCAGT CGTCCAGCCC 
CAGCCCCTGG TGGTAGTAAA GGAGGAGAAG ATTCACTCAC CAATCATTCG CAGCGAGCCT 
TTCAGCACCT CACTTCGACC AGAGCCCCCC AAGCACCCAG AGAACATCAA GGCCCCAGTC 
CACCTGCCCC AGCGGCCTGA GATGAAGCCT GTAGACATAG GGAGGCCTGT GATCCGGCCT 
CCAGAGCAGA GTGCACCCCC ACCAGGGGCC CCTGACAAGG ACAAACAGAA GCAGGAGCCA 
AAAACACCAG TGGCACCCAA AAAGGACCTG AAAATTAAGA ACATGGGCTC CTGGGCCAGC 
CTGGTACAGA AGCATCCGAC CACCCCATCC TCCACAGCCA AGTCCTCAAG TGACAGCTTT 
GAGCATTTCC GCCGTGCTGC TCGGGAGAAG GAGGAGAGGG AAAAGGCCCT GAAGGCTCAG 
GCTGAGCATG CAGAGAAGGA GAAGGAGCGG CTGAGGCAGG AGCGCATGAG GAGCCGAGAG 
GATGAAGATG CGCTGGAACA GGCACGTCGA GCCCATGAGG AGGCACGACG GCGCCAGGAG 
CAGCAGCAGC AGCAGCAGCA GCAGAGACAG GAGCAGCAGC AGCAGCAGCA GCAGGCAGCT 
GCTGTGGCCG CTGCCTCTGC CCCCCAGGCC CAGAGCTCCC AGCCCCAGTC TATGCTGGAC 
CAGCAGAGGG AGTTGGCCCG GAAACGAGAG CAGGAGCGGA GGCGCAGGGA GGCAATGGCA 
GCTACAATTG ACATGAATTT CCAGAGTGAT CTTTTGTCAA TATTTGAAGA AAATCTTTTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524752.1
CDS	421..4623
Translation	MSTESGPGTRLRNLPVMGDGLETSQMSTTQAQAQPQPANAASTNPPPPETSNPNKPKRQTNQLQYLLRVVLKTLWKHQFAWPFQQPVDAVKLNLPDYYKIIKTPMDMGTIKKRLENNYYWNAQECIQDFNTMFTNCYIYNKPGDDIVLMAEALEKLFLQKINELPTEETEIMIVQAKGRGRGRKETGAAKPGVSTVPNTTQASTSPQTQTPQQNPPPPVQATTHPFPAVTPDLIAQPPVMTMVPPQPLQTPSPVPPQPPPPPAPVPQPVQSHPPIIATTPQPVKTKKGVKRKADTTTPTTIDPIHEPPSLAPEPKTAKLGPRRESSRPVKPPKKDVPDSQQHPGPEKSSKISEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYCDIIKHPMDMSTIKSKLESREYRDAQEFGADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPEEPVVTVSSPAVPPPTKVVAPPSSSDSSSDSSSDSDSSTDDSEEERAQRLAELQEQLKAVHEQLAALSQPQQNKPKKKEKDKKEKKKEKHKKKEEVEENKKSKTKELPPKKTKKNNSSNSNVSKKEPVPTKTKPPPTYESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVDVIAGSSKMKGFSSSESESTSESSSSDSEDSETEMAPKSKKKGHTGRDQKKHHHHHHPQMQPAPAPVPQQPPPPPQQPPPPPPPQQQQQQPPPPPPPPSMPQQTAPAMKSSPPPFITAQVPVLEPQLPGSVFDPIGHFTQPILHLPQPELPPHLPQPPEHSTPPHLNQHAVVSPPALHNALPQQPSRPSNRAAALPPKPTRPPAVSPALAQPPLLPQPPMAQPPQVLLEDEEPPAPPLTSMQMQLYLQQLQKVQPPTPLLPSVKVQSQPPPPLPPPPHPSVQQQQLQPQPPPPPPPQPQPPPQQQHQPPPRPVHLPSMPFSAHIQQPPPPPGQQPTHPPPGQQPPPPQPAKPQQVIQHHPSPRHHKSDPYSAGHLREAPSPLMIHSPQMPQFQSLTHQSPPQQNVQPKKQVKGRAEPQPPGPVMGQGQGCPPASPAAVPMLSQELRPPSVVQPQPLVVVKEEKIHSPIIRSEPFSTSLRPEPPKHPENIKAPVHLPQRPEMKPVDIGRPVIRPPEQSAPPPGAPDKDKQKQEPKTPVAPKKDLKIKNMGSWASLVQKHPTTPSSTAKSSSDSFEHFRRAAREKEEREKALKAQAEHAEKEKERLRQERMRSREDEDALEQARRAHEEARRRQEQQQQQQQQRQEQQQQQQQAAAVAAASAPQAQSSQPQSMLDQQRELARKREQERRRREAMAATIDMNFQSDLLSIFEENLF

Target ORF information:

RefSeq Version	XM_006524689.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus bromodomain containing 4 (Brd4), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524689.3

ORF Insert Sequence:

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGCC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGACAGACA 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCCGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAAAAA 
ATCAATGAAC TGCCTACAGA AGAAACTGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGAGGGAGGA AAGAAACAGG GGCAGCAAAG CCTGGTGTAT CCACGGTACC AAACACAACT 
CAAGCATCAA CTTCTCCGCA GACCCAGACG CCTCAGCAGA ACCCTCCTCC ACCTGTGCAG 
GCCACAACTC ACCCCTTTCC TGCTGTCACC CCAGACCTCA TTGCCCAGCC TCCTGTCATG 
ACAATGGTGC CCCCTCAGCC ACTTCAGACT CCTTCACCGG TACCCCCCCA GCCACCACCC 
CCACCTGCTC CAGTTCCACA GCCTGTGCAG AGTCACCCGC CCATCATTGC GACCACCCCC 
CAGCCTGTGA AGACAAAGAA AGGGGTGAAG AGGAAAGCAG ATACCACCAC CCCTACCACC 
ATCGACCCCA TTCATGAGCC ACCCTCACTG GCCCCAGAGC CCAAGACCGC CAAGCTGGGT 
CCTCGGCGGG AGAGCAGCAG ACCTGTGAAG CCTCCAAAGA AGGATGTACC GGACTCACAG 
CAGCACCCAG GGCCAGAGAA GAGCAGCAAG ATCTCTGAGC AGCTAAAGTG CTGCAGTGGC 
ATCCTCAAGG AGATGTTTGC CAAGAAACAT GCTGCCTATG CCTGGCCTTT CTACAAGCCT 
GTGGATGTGG AGGCACTGGG TCTGCACGAC TACTGTGACA TCATCAAACA TCCCATGGAC 
ATGAGCACAA TCAAGTCTAA ACTAGAGTCC CGAGAGTACA GAGATGCCCA GGAATTTGGT 
GCTGATGTCC GATTGATGTT CTCCAACTGC TACAAGTACA ACCCCCCTGA CCATGAAGTG 
GTAGCCATGG CTCGAAAACT CCAGGATGTG TTTGAAATGC GCTTTGCCAA GATGCCTGAT 
GAGCCTGAAG AGCCAGTTGT TACAGTGTCC TCTCCTGCAG TGCCACCCCC TACAAAGGTG 
GTAGCCCCAC CCTCATCTAG TGACAGCAGC AGCGACAGTT CTTCCGACAG CGACAGTTCC 
ACTGACGACT CTGAGGAAGA GCGAGCCCAG CGGCTGGCTG AACTCCAGGA ACAGCTCAAG 
GCCGTGCATG AGCAGCTTGC AGCCCTCTCA CAGCCCCAGC AGAACAAACC AAAGAAAAAG 
GAGAAGGACA AGAAGGAAAA GAAAAAGGAA AAGCACAAAA AGAAAGAAGA AGTGGAGGAA 
AATAAAAAAA GCAAAACCAA GGAACTTCCT CCCAAAAAGA CAAAGAAAAA TAACAGCAGC 
AACAGCAATG TGAGCAAGAA GGAACCAGTA CCCACGAAGA CCAAGCCGCC TCCCACATAT 
GAATCAGAAG AGGAGGATAA GTGTAAGCCC ATGTCTTATG AGGAGAAGCG GCAGCTAAGT 
CTAGATATCA ACAAACTTCC TGGTGAGAAG CTAGGCCGTG TAGTACACAT AATTCAGTCA 
AGGGAACCAT CACTTAAAAA CTCCAACCCC GATGAGATTG AGATTGACTT TGAGACCCTG 
AAGCCATCTA CACTACGAGA GTTGGAGCGA TATGTCACCT CCTGTTTGCG GAAGAAAAGG 
AAACCTCAAG CTGAAAAAGT TGACGTGATT GCTGGTTCTT CCAAGATGAA GGGATTCTCA 
TCCTCTGAGT CGGAGAGCAC CAGCGAATCC AGCTCCTCTG ACAGTGAAGA CTCTGAAACA 
GAGATGGCTC CCAAGTCAAA AAAGAAGGGG CACACTGGGA GGGACCAGAA AAAGCATCAT 
CACCATCACC ATCCACAGAT GCAGCCAGCC CCAGCTCCTG TGCCCCAGCA GCCGCCCCCA 
CCTCCACAGC AGCCTCCTCC ACCCCCACCT CCGCAGCAGC AGCAGCAGCA ACCTCCACCC 
CCACCACCTC CGCCCTCCAT GCCACAGCAG ACTGCCCCAG CGATGAAGTC CTCGCCCCCA 
CCCTTCATCA CTGCCCAGGT CCCCGTCCTG GAACCACAGC TGCCAGGCAG TGTCTTTGAC 
CCTATTGGCC ACTTCACCCA GCCCATCTTG CACCTGCCGC AGCCGGAGCT GCCTCCTCAC 
CTGCCCCAGC CACCTGAGCA CAGCACTCCA CCCCATCTCA ACCAGCATGC TGTGGTCTCT 
CCTCCAGCTT TGCACAATGC GCTGCCCCAA CAGCCATCTC GGCCCAGTAA CCGAGCTGCT 
GCTCTGCCCC CAAAGCCTAC CCGACCCCCA GCTGTGTCCC CTGCCCTGGC CCAGCCCCCC 
CTGCTCCCAC AACCACCAAT GGCTCAGCCC CCCCAAGTGC TGCTGGAGGA TGAAGAGCCA 
CCTGCCCCAC CCCTCACCTC CATGCAGATG CAGTTGTACC TACAGCAGCT GCAGAAGGTG 
CAGCCTCCCA CACCACTACT CCCTTCCGTG AAGGTGCAGT CCCAACCCCC ACCGCCTTTG 
CCGCCTCCAC CCCACCCTTC TGTGCAGCAG CAGCAGCTCC AGCCACAGCC ACCGCCACCC 
CCACCTCCTC AGCCACAGCC ACCACCCCAG CAACAACACC AGCCTCCACC ACGACCAGTT 
CACTTGCCAT CCATGCCCTT TTCTGCTCAT ATTCAGCAGC CCCCACCACC CCCAGGACAG 
CAACCTACTC ACCCACCCCC AGGACAGCAG CCCCCACCAC CACAGCCTGC CAAGCCCCAG 
CAAGTCATCC AGCATCACCC TTCCCCCCGG CACCACAAGT CAGACCCCTA CTCAGCTGGT 
CATCTTCGTG AGGCTCCCTC TCCGCTTATG ATACATTCCC CTCAGATGCC ACAGTTCCAG 
AGCCTGACCC ATCAGTCTCC TCCCCAGCAA AACGTCCAGC CTAAGAAGCA GGTAAAGGGC 
AGGGCTGAGC CACAGCCACC AGGGCCAGTC ATGGGCCAAG GCCAGGGATG CCCACCTGCC 
TCACCGGCTG CCGTGCCTAT GCTGTCCCAG GAGCTGCGAC CACCTTCAGT CGTCCAGCCC 
CAGCCCCTGG TGGTAGTAAA GGAGGAGAAG ATTCACTCAC CAATCATTCG CAGCGAGCCT 
TTCAGCACCT CACTTCGACC AGAGCCCCCC AAGCACCCAG AGAACATCAA GGCCCCAGTC 
CACCTGCCCC AGCGGCCTGA GATGAAGCCT GTAGACATAG GGAGGCCTGT GATCCGGCCT 
CCAGAGCAGA GTGCACCCCC ACCAGGGGCC CCTGACAAGG ACAAACAGAA GCAGGAGCCA 
AAAACACCAG TGGCACCCAA AAAGGACCTG AAAATTAAGA ACATGGGCTC CTGGGCCAGC 
CTGGTACAGA AGCATCCGAC CACCCCATCC TCCACAGCCA AGTCCTCAAG TGACAGCTTT 
GAGCATTTCC GCCGTGCTGC TCGGGAGAAG GAGGAGAGGG AAAAGGCCCT GAAGGCTCAG 
GCTGAGCATG CAGAGAAGGA GAAGGAGCGG CTGAGGCAGG AGCGCATGAG GAGCCGAGAG 
GATGAAGATG CGCTGGAACA GGCACGTCGA GCCCATGAGG AGGCACGACG GCGCCAGGAG 
CAGCAGCAGC AGCAGCAGCA GCAGAGACAG GAGCAGCAGC AGCAGCAGCA GCAGGCAGCT 
GCTGTGGCCG CTGCCTCTGC CCCCCAGGCC CAGAGCTCCC AGCCCCAGTC TATGCTGGAC 
CAGCAGAGGG AGTTGGCCCG GAAACGAGAG CAGGAGCGGA GGCGCAGGGA GGCAATGGCA 
GCTACAATTG ACATGAATTT CCAGAGTGAT CTTTTGTCAA TATTTGAAGA AAATCTTTTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brd4 ( XM_030249924.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105784.1 Mus musculus bromodomain containing 4 (Brd4), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OMu16383
Clone ID Related Accession (Same CDS sequence)	XM_030249927.1 , XM_030249925.1 , XM_006524689.2 , NM_020508.4 , XM_006524688.1 , XM_006524688.2 , XM_030249926.1 , XM_006524689.3 , XM_030249924.1
Accession Version	XM_030249924.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4203bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	bromodomain-containing protein 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGCC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGACAGACA 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCCGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAAAAA 
ATCAATGAAC TGCCTACAGA AGAAACTGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGAGGGAGGA AAGAAACAGG GGCAGCAAAG CCTGGTGTAT CCACGGTACC AAACACAACT 
CAAGCATCAA CTTCTCCGCA GACCCAGACG CCTCAGCAGA ACCCTCCTCC ACCTGTGCAG 
GCCACAACTC ACCCCTTTCC TGCTGTCACC CCAGACCTCA TTGCCCAGCC TCCTGTCATG 
ACAATGGTGC CCCCTCAGCC ACTTCAGACT CCTTCACCGG TACCCCCCCA GCCACCACCC 
CCACCTGCTC CAGTTCCACA GCCTGTGCAG AGTCACCCGC CCATCATTGC GACCACCCCC 
CAGCCTGTGA AGACAAAGAA AGGGGTGAAG AGGAAAGCAG ATACCACCAC CCCTACCACC 
ATCGACCCCA TTCATGAGCC ACCCTCACTG GCCCCAGAGC CCAAGACCGC CAAGCTGGGT 
CCTCGGCGGG AGAGCAGCAG ACCTGTGAAG CCTCCAAAGA AGGATGTACC GGACTCACAG 
CAGCACCCAG GGCCAGAGAA GAGCAGCAAG ATCTCTGAGC AGCTAAAGTG CTGCAGTGGC 
ATCCTCAAGG AGATGTTTGC CAAGAAACAT GCTGCCTATG CCTGGCCTTT CTACAAGCCT 
GTGGATGTGG AGGCACTGGG TCTGCACGAC TACTGTGACA TCATCAAACA TCCCATGGAC 
ATGAGCACAA TCAAGTCTAA ACTAGAGTCC CGAGAGTACA GAGATGCCCA GGAATTTGGT 
GCTGATGTCC GATTGATGTT CTCCAACTGC TACAAGTACA ACCCCCCTGA CCATGAAGTG 
GTAGCCATGG CTCGAAAACT CCAGGATGTG TTTGAAATGC GCTTTGCCAA GATGCCTGAT 
GAGCCTGAAG AGCCAGTTGT TACAGTGTCC TCTCCTGCAG TGCCACCCCC TACAAAGGTG 
GTAGCCCCAC CCTCATCTAG TGACAGCAGC AGCGACAGTT CTTCCGACAG CGACAGTTCC 
ACTGACGACT CTGAGGAAGA GCGAGCCCAG CGGCTGGCTG AACTCCAGGA ACAGCTCAAG 
GCCGTGCATG AGCAGCTTGC AGCCCTCTCA CAGCCCCAGC AGAACAAACC AAAGAAAAAG 
GAGAAGGACA AGAAGGAAAA GAAAAAGGAA AAGCACAAAA AGAAAGAAGA AGTGGAGGAA 
AATAAAAAAA GCAAAACCAA GGAACTTCCT CCCAAAAAGA CAAAGAAAAA TAACAGCAGC 
AACAGCAATG TGAGCAAGAA GGAACCAGTA CCCACGAAGA CCAAGCCGCC TCCCACATAT 
GAATCAGAAG AGGAGGATAA GTGTAAGCCC ATGTCTTATG AGGAGAAGCG GCAGCTAAGT 
CTAGATATCA ACAAACTTCC TGGTGAGAAG CTAGGCCGTG TAGTACACAT AATTCAGTCA 
AGGGAACCAT CACTTAAAAA CTCCAACCCC GATGAGATTG AGATTGACTT TGAGACCCTG 
AAGCCATCTA CACTACGAGA GTTGGAGCGA TATGTCACCT CCTGTTTGCG GAAGAAAAGG 
AAACCTCAAG CTGAAAAAGT TGACGTGATT GCTGGTTCTT CCAAGATGAA GGGATTCTCA 
TCCTCTGAGT CGGAGAGCAC CAGCGAATCC AGCTCCTCTG ACAGTGAAGA CTCTGAAACA 
GAGATGGCTC CCAAGTCAAA AAAGAAGGGG CACACTGGGA GGGACCAGAA AAAGCATCAT 
CACCATCACC ATCCACAGAT GCAGCCAGCC CCAGCTCCTG TGCCCCAGCA GCCGCCCCCA 
CCTCCACAGC AGCCTCCTCC ACCCCCACCT CCGCAGCAGC AGCAGCAGCA ACCTCCACCC 
CCACCACCTC CGCCCTCCAT GCCACAGCAG ACTGCCCCAG CGATGAAGTC CTCGCCCCCA 
CCCTTCATCA CTGCCCAGGT CCCCGTCCTG GAACCACAGC TGCCAGGCAG TGTCTTTGAC 
CCTATTGGCC ACTTCACCCA GCCCATCTTG CACCTGCCGC AGCCGGAGCT GCCTCCTCAC 
CTGCCCCAGC CACCTGAGCA CAGCACTCCA CCCCATCTCA ACCAGCATGC TGTGGTCTCT 
CCTCCAGCTT TGCACAATGC GCTGCCCCAA CAGCCATCTC GGCCCAGTAA CCGAGCTGCT 
GCTCTGCCCC CAAAGCCTAC CCGACCCCCA GCTGTGTCCC CTGCCCTGGC CCAGCCCCCC 
CTGCTCCCAC AACCACCAAT GGCTCAGCCC CCCCAAGTGC TGCTGGAGGA TGAAGAGCCA 
CCTGCCCCAC CCCTCACCTC CATGCAGATG CAGTTGTACC TACAGCAGCT GCAGAAGGTG 
CAGCCTCCCA CACCACTACT CCCTTCCGTG AAGGTGCAGT CCCAACCCCC ACCGCCTTTG 
CCGCCTCCAC CCCACCCTTC TGTGCAGCAG CAGCAGCTCC AGCCACAGCC ACCGCCACCC 
CCACCTCCTC AGCCACAGCC ACCACCCCAG CAACAACACC AGCCTCCACC ACGACCAGTT 
CACTTGCCAT CCATGCCCTT TTCTGCTCAT ATTCAGCAGC CCCCACCACC CCCAGGACAG 
CAACCTACTC ACCCACCCCC AGGACAGCAG CCCCCACCAC CACAGCCTGC CAAGCCCCAG 
CAAGTCATCC AGCATCACCC TTCCCCCCGG CACCACAAGT CAGACCCCTA CTCAGCTGGT 
CATCTTCGTG AGGCTCCCTC TCCGCTTATG ATACATTCCC CTCAGATGCC ACAGTTCCAG 
AGCCTGACCC ATCAGTCTCC TCCCCAGCAA AACGTCCAGC CTAAGAAGCA GGTAAAGGGC 
AGGGCTGAGC CACAGCCACC AGGGCCAGTC ATGGGCCAAG GCCAGGGATG CCCACCTGCC 
TCACCGGCTG CCGTGCCTAT GCTGTCCCAG GAGCTGCGAC CACCTTCAGT CGTCCAGCCC 
CAGCCCCTGG TGGTAGTAAA GGAGGAGAAG ATTCACTCAC CAATCATTCG CAGCGAGCCT 
TTCAGCACCT CACTTCGACC AGAGCCCCCC AAGCACCCAG AGAACATCAA GGCCCCAGTC 
CACCTGCCCC AGCGGCCTGA GATGAAGCCT GTAGACATAG GGAGGCCTGT GATCCGGCCT 
CCAGAGCAGA GTGCACCCCC ACCAGGGGCC CCTGACAAGG ACAAACAGAA GCAGGAGCCA 
AAAACACCAG TGGCACCCAA AAAGGACCTG AAAATTAAGA ACATGGGCTC CTGGGCCAGC 
CTGGTACAGA AGCATCCGAC CACCCCATCC TCCACAGCCA AGTCCTCAAG TGACAGCTTT 
GAGCATTTCC GCCGTGCTGC TCGGGAGAAG GAGGAGAGGG AAAAGGCCCT GAAGGCTCAG 
GCTGAGCATG CAGAGAAGGA GAAGGAGCGG CTGAGGCAGG AGCGCATGAG GAGCCGAGAG 
GATGAAGATG CGCTGGAACA GGCACGTCGA GCCCATGAGG AGGCACGACG GCGCCAGGAG 
CAGCAGCAGC AGCAGCAGCA GCAGAGACAG GAGCAGCAGC AGCAGCAGCA GCAGGCAGCT 
GCTGTGGCCG CTGCCTCTGC CCCCCAGGCC CAGAGCTCCC AGCCCCAGTC TATGCTGGAC 
CAGCAGAGGG AGTTGGCCCG GAAACGAGAG CAGGAGCGGA GGCGCAGGGA GGCAATGGCA 
GCTACAATTG ACATGAATTT CCAGAGTGAT CTTTTGTCAA TATTTGAAGA AAATCTTTTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105784.1
CDS	407..4609
Translation	MSTESGPGTRLRNLPVMGDGLETSQMSTTQAQAQPQPANAASTNPPPPETSNPNKPKRQTNQLQYLLRVVLKTLWKHQFAWPFQQPVDAVKLNLPDYYKIIKTPMDMGTIKKRLENNYYWNAQECIQDFNTMFTNCYIYNKPGDDIVLMAEALEKLFLQKINELPTEETEIMIVQAKGRGRGRKETGAAKPGVSTVPNTTQASTSPQTQTPQQNPPPPVQATTHPFPAVTPDLIAQPPVMTMVPPQPLQTPSPVPPQPPPPPAPVPQPVQSHPPIIATTPQPVKTKKGVKRKADTTTPTTIDPIHEPPSLAPEPKTAKLGPRRESSRPVKPPKKDVPDSQQHPGPEKSSKISEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYCDIIKHPMDMSTIKSKLESREYRDAQEFGADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPEEPVVTVSSPAVPPPTKVVAPPSSSDSSSDSSSDSDSSTDDSEEERAQRLAELQEQLKAVHEQLAALSQPQQNKPKKKEKDKKEKKKEKHKKKEEVEENKKSKTKELPPKKTKKNNSSNSNVSKKEPVPTKTKPPPTYESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVDVIAGSSKMKGFSSSESESTSESSSSDSEDSETEMAPKSKKKGHTGRDQKKHHHHHHPQMQPAPAPVPQQPPPPPQQPPPPPPPQQQQQQPPPPPPPPSMPQQTAPAMKSSPPPFITAQVPVLEPQLPGSVFDPIGHFTQPILHLPQPELPPHLPQPPEHSTPPHLNQHAVVSPPALHNALPQQPSRPSNRAAALPPKPTRPPAVSPALAQPPLLPQPPMAQPPQVLLEDEEPPAPPLTSMQMQLYLQQLQKVQPPTPLLPSVKVQSQPPPPLPPPPHPSVQQQQLQPQPPPPPPPQPQPPPQQQHQPPPRPVHLPSMPFSAHIQQPPPPPGQQPTHPPPGQQPPPPQPAKPQQVIQHHPSPRHHKSDPYSAGHLREAPSPLMIHSPQMPQFQSLTHQSPPQQNVQPKKQVKGRAEPQPPGPVMGQGQGCPPASPAAVPMLSQELRPPSVVQPQPLVVVKEEKIHSPIIRSEPFSTSLRPEPPKHPENIKAPVHLPQRPEMKPVDIGRPVIRPPEQSAPPPGAPDKDKQKQEPKTPVAPKKDLKIKNMGSWASLVQKHPTTPSSTAKSSSDSFEHFRRAAREKEEREKALKAQAEHAEKEKERLRQERMRSREDEDALEQARRAHEEARRRQEQQQQQQQQRQEQQQQQQQAAAVAAASAPQAQSSQPQSMLDQQRELARKREQERRRREAMAATIDMNFQSDLLSIFEENLF

Target ORF information:

RefSeq Version	XM_030249924.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus bromodomain containing 4 (Brd4), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249924.1

ORF Insert Sequence:

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGCC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGACAGACA 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCCGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAAAAA 
ATCAATGAAC TGCCTACAGA AGAAACTGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGAGGGAGGA AAGAAACAGG GGCAGCAAAG CCTGGTGTAT CCACGGTACC AAACACAACT 
CAAGCATCAA CTTCTCCGCA GACCCAGACG CCTCAGCAGA ACCCTCCTCC ACCTGTGCAG 
GCCACAACTC ACCCCTTTCC TGCTGTCACC CCAGACCTCA TTGCCCAGCC TCCTGTCATG 
ACAATGGTGC CCCCTCAGCC ACTTCAGACT CCTTCACCGG TACCCCCCCA GCCACCACCC 
CCACCTGCTC CAGTTCCACA GCCTGTGCAG AGTCACCCGC CCATCATTGC GACCACCCCC 
CAGCCTGTGA AGACAAAGAA AGGGGTGAAG AGGAAAGCAG ATACCACCAC CCCTACCACC 
ATCGACCCCA TTCATGAGCC ACCCTCACTG GCCCCAGAGC CCAAGACCGC CAAGCTGGGT 
CCTCGGCGGG AGAGCAGCAG ACCTGTGAAG CCTCCAAAGA AGGATGTACC GGACTCACAG 
CAGCACCCAG GGCCAGAGAA GAGCAGCAAG ATCTCTGAGC AGCTAAAGTG CTGCAGTGGC 
ATCCTCAAGG AGATGTTTGC CAAGAAACAT GCTGCCTATG CCTGGCCTTT CTACAAGCCT 
GTGGATGTGG AGGCACTGGG TCTGCACGAC TACTGTGACA TCATCAAACA TCCCATGGAC 
ATGAGCACAA TCAAGTCTAA ACTAGAGTCC CGAGAGTACA GAGATGCCCA GGAATTTGGT 
GCTGATGTCC GATTGATGTT CTCCAACTGC TACAAGTACA ACCCCCCTGA CCATGAAGTG 
GTAGCCATGG CTCGAAAACT CCAGGATGTG TTTGAAATGC GCTTTGCCAA GATGCCTGAT 
GAGCCTGAAG AGCCAGTTGT TACAGTGTCC TCTCCTGCAG TGCCACCCCC TACAAAGGTG 
GTAGCCCCAC CCTCATCTAG TGACAGCAGC AGCGACAGTT CTTCCGACAG CGACAGTTCC 
ACTGACGACT CTGAGGAAGA GCGAGCCCAG CGGCTGGCTG AACTCCAGGA ACAGCTCAAG 
GCCGTGCATG AGCAGCTTGC AGCCCTCTCA CAGCCCCAGC AGAACAAACC AAAGAAAAAG 
GAGAAGGACA AGAAGGAAAA GAAAAAGGAA AAGCACAAAA AGAAAGAAGA AGTGGAGGAA 
AATAAAAAAA GCAAAACCAA GGAACTTCCT CCCAAAAAGA CAAAGAAAAA TAACAGCAGC 
AACAGCAATG TGAGCAAGAA GGAACCAGTA CCCACGAAGA CCAAGCCGCC TCCCACATAT 
GAATCAGAAG AGGAGGATAA GTGTAAGCCC ATGTCTTATG AGGAGAAGCG GCAGCTAAGT 
CTAGATATCA ACAAACTTCC TGGTGAGAAG CTAGGCCGTG TAGTACACAT AATTCAGTCA 
AGGGAACCAT CACTTAAAAA CTCCAACCCC GATGAGATTG AGATTGACTT TGAGACCCTG 
AAGCCATCTA CACTACGAGA GTTGGAGCGA TATGTCACCT CCTGTTTGCG GAAGAAAAGG 
AAACCTCAAG CTGAAAAAGT TGACGTGATT GCTGGTTCTT CCAAGATGAA GGGATTCTCA 
TCCTCTGAGT CGGAGAGCAC CAGCGAATCC AGCTCCTCTG ACAGTGAAGA CTCTGAAACA 
GAGATGGCTC CCAAGTCAAA AAAGAAGGGG CACACTGGGA GGGACCAGAA AAAGCATCAT 
CACCATCACC ATCCACAGAT GCAGCCAGCC CCAGCTCCTG TGCCCCAGCA GCCGCCCCCA 
CCTCCACAGC AGCCTCCTCC ACCCCCACCT CCGCAGCAGC AGCAGCAGCA ACCTCCACCC 
CCACCACCTC CGCCCTCCAT GCCACAGCAG ACTGCCCCAG CGATGAAGTC CTCGCCCCCA 
CCCTTCATCA CTGCCCAGGT CCCCGTCCTG GAACCACAGC TGCCAGGCAG TGTCTTTGAC 
CCTATTGGCC ACTTCACCCA GCCCATCTTG CACCTGCCGC AGCCGGAGCT GCCTCCTCAC 
CTGCCCCAGC CACCTGAGCA CAGCACTCCA CCCCATCTCA ACCAGCATGC TGTGGTCTCT 
CCTCCAGCTT TGCACAATGC GCTGCCCCAA CAGCCATCTC GGCCCAGTAA CCGAGCTGCT 
GCTCTGCCCC CAAAGCCTAC CCGACCCCCA GCTGTGTCCC CTGCCCTGGC CCAGCCCCCC 
CTGCTCCCAC AACCACCAAT GGCTCAGCCC CCCCAAGTGC TGCTGGAGGA TGAAGAGCCA 
CCTGCCCCAC CCCTCACCTC CATGCAGATG CAGTTGTACC TACAGCAGCT GCAGAAGGTG 
CAGCCTCCCA CACCACTACT CCCTTCCGTG AAGGTGCAGT CCCAACCCCC ACCGCCTTTG 
CCGCCTCCAC CCCACCCTTC TGTGCAGCAG CAGCAGCTCC AGCCACAGCC ACCGCCACCC 
CCACCTCCTC AGCCACAGCC ACCACCCCAG CAACAACACC AGCCTCCACC ACGACCAGTT 
CACTTGCCAT CCATGCCCTT TTCTGCTCAT ATTCAGCAGC CCCCACCACC CCCAGGACAG 
CAACCTACTC ACCCACCCCC AGGACAGCAG CCCCCACCAC CACAGCCTGC CAAGCCCCAG 
CAAGTCATCC AGCATCACCC TTCCCCCCGG CACCACAAGT CAGACCCCTA CTCAGCTGGT 
CATCTTCGTG AGGCTCCCTC TCCGCTTATG ATACATTCCC CTCAGATGCC ACAGTTCCAG 
AGCCTGACCC ATCAGTCTCC TCCCCAGCAA AACGTCCAGC CTAAGAAGCA GGTAAAGGGC 
AGGGCTGAGC CACAGCCACC AGGGCCAGTC ATGGGCCAAG GCCAGGGATG CCCACCTGCC 
TCACCGGCTG CCGTGCCTAT GCTGTCCCAG GAGCTGCGAC CACCTTCAGT CGTCCAGCCC 
CAGCCCCTGG TGGTAGTAAA GGAGGAGAAG ATTCACTCAC CAATCATTCG CAGCGAGCCT 
TTCAGCACCT CACTTCGACC AGAGCCCCCC AAGCACCCAG AGAACATCAA GGCCCCAGTC 
CACCTGCCCC AGCGGCCTGA GATGAAGCCT GTAGACATAG GGAGGCCTGT GATCCGGCCT 
CCAGAGCAGA GTGCACCCCC ACCAGGGGCC CCTGACAAGG ACAAACAGAA GCAGGAGCCA 
AAAACACCAG TGGCACCCAA AAAGGACCTG AAAATTAAGA ACATGGGCTC CTGGGCCAGC 
CTGGTACAGA AGCATCCGAC CACCCCATCC TCCACAGCCA AGTCCTCAAG TGACAGCTTT 
GAGCATTTCC GCCGTGCTGC TCGGGAGAAG GAGGAGAGGG AAAAGGCCCT GAAGGCTCAG 
GCTGAGCATG CAGAGAAGGA GAAGGAGCGG CTGAGGCAGG AGCGCATGAG GAGCCGAGAG 
GATGAAGATG CGCTGGAACA GGCACGTCGA GCCCATGAGG AGGCACGACG GCGCCAGGAG 
CAGCAGCAGC AGCAGCAGCA GCAGAGACAG GAGCAGCAGC AGCAGCAGCA GCAGGCAGCT 
GCTGTGGCCG CTGCCTCTGC CCCCCAGGCC CAGAGCTCCC AGCCCCAGTC TATGCTGGAC 
CAGCAGAGGG AGTTGGCCCG GAAACGAGAG CAGGAGCGGA GGCGCAGGGA GGCAATGGCA 
GCTACAATTG ACATGAATTT CCAGAGTGAT CTTTTGTCAA TATTTGAAGA AAATCTTTTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brd4 ( XM_006524683.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524746.1 Mus musculus bromodomain containing 4 (Brd4), transcript variant X1, mRNA.

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CloneID	OMu17143
Clone ID Related Accession (Same CDS sequence)	XM_006524683.2 , XM_006524683.3 , XM_006524684.2 , XM_030249922.1 , XM_006524686.2 , XM_006524687.2 , XM_006524685.2 , XM_030249923.1 , NM_001286630.1 , XM_006524685.1 , XM_006524686.1 , XM_006524684.3
Accession Version	XM_006524683.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4206bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	bromodomain-containing protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006524683.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGCC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGACAGACA 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCCGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAAAAA 
ATCAATGAAC TGCCTACAGA AGAAACTGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGAGGGAGGA AAGAAACAGG TAGGGCAGCA AAGCCTGGTG TATCCACGGT ACCAAACACA 
ACTCAAGCAT CAACTTCTCC GCAGACCCAG ACGCCTCAGC AGAACCCTCC TCCACCTGTG 
CAGGCCACAA CTCACCCCTT TCCTGCTGTC ACCCCAGACC TCATTGCCCA GCCTCCTGTC 
ATGACAATGG TGCCCCCTCA GCCACTTCAG ACTCCTTCAC CGGTACCCCC CCAGCCACCA 
CCCCCACCTG CTCCAGTTCC ACAGCCTGTG CAGAGTCACC CGCCCATCAT TGCGACCACC 
CCCCAGCCTG TGAAGACAAA GAAAGGGGTG AAGAGGAAAG CAGATACCAC CACCCCTACC 
ACCATCGACC CCATTCATGA GCCACCCTCA CTGGCCCCAG AGCCCAAGAC CGCCAAGCTG 
GGTCCTCGGC GGGAGAGCAG CAGACCTGTG AAGCCTCCAA AGAAGGATGT ACCGGACTCA 
CAGCAGCACC CAGGGCCAGA GAAGAGCAGC AAGATCTCTG AGCAGCTAAA GTGCTGCAGT 
GGCATCCTCA AGGAGATGTT TGCCAAGAAA CATGCTGCCT ATGCCTGGCC TTTCTACAAG 
CCTGTGGATG TGGAGGCACT GGGTCTGCAC GACTACTGTG ACATCATCAA ACATCCCATG 
GACATGAGCA CAATCAAGTC TAAACTAGAG TCCCGAGAGT ACAGAGATGC CCAGGAATTT 
GGTGCTGATG TCCGATTGAT GTTCTCCAAC TGCTACAAGT ACAACCCCCC TGACCATGAA 
GTGGTAGCCA TGGCTCGAAA ACTCCAGGAT GTGTTTGAAA TGCGCTTTGC CAAGATGCCT 
GATGAGCCTG AAGAGCCAGT TGTTACAGTG TCCTCTCCTG CAGTGCCACC CCCTACAAAG 
GTGGTAGCCC CACCCTCATC TAGTGACAGC AGCAGCGACA GTTCTTCCGA CAGCGACAGT 
TCCACTGACG ACTCTGAGGA AGAGCGAGCC CAGCGGCTGG CTGAACTCCA GGAACAGCTC 
AAGGCCGTGC ATGAGCAGCT TGCAGCCCTC TCACAGCCCC AGCAGAACAA ACCAAAGAAA 
AAGGAGAAGG ACAAGAAGGA AAAGAAAAAG GAAAAGCACA AAAAGAAAGA AGAAGTGGAG 
GAAAATAAAA AAAGCAAAAC CAAGGAACTT CCTCCCAAAA AGACAAAGAA AAATAACAGC 
AGCAACAGCA ATGTGAGCAA GAAGGAACCA GTACCCACGA AGACCAAGCC GCCTCCCACA 
TATGAATCAG AAGAGGAGGA TAAGTGTAAG CCCATGTCTT ATGAGGAGAA GCGGCAGCTA 
AGTCTAGATA TCAACAAACT TCCTGGTGAG AAGCTAGGCC GTGTAGTACA CATAATTCAG 
TCAAGGGAAC CATCACTTAA AAACTCCAAC CCCGATGAGA TTGAGATTGA CTTTGAGACC 
CTGAAGCCAT CTACACTACG AGAGTTGGAG CGATATGTCA CCTCCTGTTT GCGGAAGAAA 
AGGAAACCTC AAGCTGAAAA AGTTGACGTG ATTGCTGGTT CTTCCAAGAT GAAGGGATTC 
TCATCCTCTG AGTCGGAGAG CACCAGCGAA TCCAGCTCCT CTGACAGTGA AGACTCTGAA 
ACAGAGATGG CTCCCAAGTC AAAAAAGAAG GGGCACACTG GGAGGGACCA GAAAAAGCAT 
CATCACCATC ACCATCCACA GATGCAGCCA GCCCCAGCTC CTGTGCCCCA GCAGCCGCCC 
CCACCTCCAC AGCAGCCTCC TCCACCCCCA CCTCCGCAGC AGCAGCAGCA GCAACCTCCA 
CCCCCACCAC CTCCGCCCTC CATGCCACAG CAGACTGCCC CAGCGATGAA GTCCTCGCCC 
CCACCCTTCA TCACTGCCCA GGTCCCCGTC CTGGAACCAC AGCTGCCAGG CAGTGTCTTT 
GACCCTATTG GCCACTTCAC CCAGCCCATC TTGCACCTGC CGCAGCCGGA GCTGCCTCCT 
CACCTGCCCC AGCCACCTGA GCACAGCACT CCACCCCATC TCAACCAGCA TGCTGTGGTC 
TCTCCTCCAG CTTTGCACAA TGCGCTGCCC CAACAGCCAT CTCGGCCCAG TAACCGAGCT 
GCTGCTCTGC CCCCAAAGCC TACCCGACCC CCAGCTGTGT CCCCTGCCCT GGCCCAGCCC 
CCCCTGCTCC CACAACCACC AATGGCTCAG CCCCCCCAAG TGCTGCTGGA GGATGAAGAG 
CCACCTGCCC CACCCCTCAC CTCCATGCAG ATGCAGTTGT ACCTACAGCA GCTGCAGAAG 
GTGCAGCCTC CCACACCACT ACTCCCTTCC GTGAAGGTGC AGTCCCAACC CCCACCGCCT 
TTGCCGCCTC CACCCCACCC TTCTGTGCAG CAGCAGCAGC TCCAGCCACA GCCACCGCCA 
CCCCCACCTC CTCAGCCACA GCCACCACCC CAGCAACAAC ACCAGCCTCC ACCACGACCA 
GTTCACTTGC CATCCATGCC CTTTTCTGCT CATATTCAGC AGCCCCCACC ACCCCCAGGA 
CAGCAACCTA CTCACCCACC CCCAGGACAG CAGCCCCCAC CACCACAGCC TGCCAAGCCC 
CAGCAAGTCA TCCAGCATCA CCCTTCCCCC CGGCACCACA AGTCAGACCC CTACTCAGCT 
GGTCATCTTC GTGAGGCTCC CTCTCCGCTT ATGATACATT CCCCTCAGAT GCCACAGTTC 
CAGAGCCTGA CCCATCAGTC TCCTCCCCAG CAAAACGTCC AGCCTAAGAA GCAGGTAAAG 
GGCAGGGCTG AGCCACAGCC ACCAGGGCCA GTCATGGGCC AAGGCCAGGG ATGCCCACCT 
GCCTCACCGG CTGCCGTGCC TATGCTGTCC CAGGAGCTGC GACCACCTTC AGTCGTCCAG 
CCCCAGCCCC TGGTGGTAGT AAAGGAGGAG AAGATTCACT CACCAATCAT TCGCAGCGAG 
CCTTTCAGCA CCTCACTTCG ACCAGAGCCC CCCAAGCACC CAGAGAACAT CAAGGCCCCA 
GTCCACCTGC CCCAGCGGCC TGAGATGAAG CCTGTAGACA TAGGGAGGCC TGTGATCCGG 
CCTCCAGAGC AGAGTGCACC CCCACCAGGG GCCCCTGACA AGGACAAACA GAAGCAGGAG 
CCAAAAACAC CAGTGGCACC CAAAAAGGAC CTGAAAATTA AGAACATGGG CTCCTGGGCC 
AGCCTGGTAC AGAAGCATCC GACCACCCCA TCCTCCACAG CCAAGTCCTC AAGTGACAGC 
TTTGAGCATT TCCGCCGTGC TGCTCGGGAG AAGGAGGAGA GGGAAAAGGC CCTGAAGGCT 
CAGGCTGAGC ATGCAGAGAA GGAGAAGGAG CGGCTGAGGC AGGAGCGCAT GAGGAGCCGA 
GAGGATGAAG ATGCGCTGGA ACAGGCACGT CGAGCCCATG AGGAGGCACG ACGGCGCCAG 
GAGCAGCAGC AGCAGCAGCA GCAGCAGAGA CAGGAGCAGC AGCAGCAGCA GCAGCAGGCA 
GCTGCTGTGG CCGCTGCCTC TGCCCCCCAG GCCCAGAGCT CCCAGCCCCA GTCTATGCTG 
GACCAGCAGA GGGAGTTGGC CCGGAAACGA GAGCAGGAGC GGAGGCGCAG GGAGGCAATG 
GCAGCTACAA TTGACATGAA TTTCCAGAGT GATCTTTTGT CAATATTTGA AGAAAATCTT 
TTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524746.1
CDS	109..4314
Translation	MSTESGPGTRLRNLPVMGDGLETSQMSTTQAQAQPQPANAASTNPPPPETSNPNKPKRQTNQLQYLLRVVLKTLWKHQFAWPFQQPVDAVKLNLPDYYKIIKTPMDMGTIKKRLENNYYWNAQECIQDFNTMFTNCYIYNKPGDDIVLMAEALEKLFLQKINELPTEETEIMIVQAKGRGRGRKETGRAAKPGVSTVPNTTQASTSPQTQTPQQNPPPPVQATTHPFPAVTPDLIAQPPVMTMVPPQPLQTPSPVPPQPPPPPAPVPQPVQSHPPIIATTPQPVKTKKGVKRKADTTTPTTIDPIHEPPSLAPEPKTAKLGPRRESSRPVKPPKKDVPDSQQHPGPEKSSKISEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYCDIIKHPMDMSTIKSKLESREYRDAQEFGADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPEEPVVTVSSPAVPPPTKVVAPPSSSDSSSDSSSDSDSSTDDSEEERAQRLAELQEQLKAVHEQLAALSQPQQNKPKKKEKDKKEKKKEKHKKKEEVEENKKSKTKELPPKKTKKNNSSNSNVSKKEPVPTKTKPPPTYESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVDVIAGSSKMKGFSSSESESTSESSSSDSEDSETEMAPKSKKKGHTGRDQKKHHHHHHPQMQPAPAPVPQQPPPPPQQPPPPPPPQQQQQQPPPPPPPPSMPQQTAPAMKSSPPPFITAQVPVLEPQLPGSVFDPIGHFTQPILHLPQPELPPHLPQPPEHSTPPHLNQHAVVSPPALHNALPQQPSRPSNRAAALPPKPTRPPAVSPALAQPPLLPQPPMAQPPQVLLEDEEPPAPPLTSMQMQLYLQQLQKVQPPTPLLPSVKVQSQPPPPLPPPPHPSVQQQQLQPQPPPPPPPQPQPPPQQQHQPPPRPVHLPSMPFSAHIQQPPPPPGQQPTHPPPGQQPPPPQPAKPQQVIQHHPSPRHHKSDPYSAGHLREAPSPLMIHSPQMPQFQSLTHQSPPQQNVQPKKQVKGRAEPQPPGPVMGQGQGCPPASPAAVPMLSQELRPPSVVQPQPLVVVKEEKIHSPIIRSEPFSTSLRPEPPKHPENIKAPVHLPQRPEMKPVDIGRPVIRPPEQSAPPPGAPDKDKQKQEPKTPVAPKKDLKIKNMGSWASLVQKHPTTPSSTAKSSSDSFEHFRRAAREKEEREKALKAQAEHAEKEKERLRQERMRSREDEDALEQARRAHEEARRRQEQQQQQQQQRQEQQQQQQQAAAVAAASAPQAQSSQPQSMLDQQRELARKREQERRRREAMAATIDMNFQSDLLSIFEENLF

Target ORF information:

RefSeq Version	XM_006524683.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus bromodomain containing 4 (Brd4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524683.2

ORF Insert Sequence:

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGCC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGACAGACA 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCCGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAAAAA 
ATCAATGAAC TGCCTACAGA AGAAACTGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGAGGGAGGA AAGAAACAGG TAGGGCAGCA AAGCCTGGTG TATCCACGGT ACCAAACACA 
ACTCAAGCAT CAACTTCTCC GCAGACCCAG ACGCCTCAGC AGAACCCTCC TCCACCTGTG 
CAGGCCACAA CTCACCCCTT TCCTGCTGTC ACCCCAGACC TCATTGCCCA GCCTCCTGTC 
ATGACAATGG TGCCCCCTCA GCCACTTCAG ACTCCTTCAC CGGTACCCCC CCAGCCACCA 
CCCCCACCTG CTCCAGTTCC ACAGCCTGTG CAGAGTCACC CGCCCATCAT TGCGACCACC 
CCCCAGCCTG TGAAGACAAA GAAAGGGGTG AAGAGGAAAG CAGATACCAC CACCCCTACC 
ACCATCGACC CCATTCATGA GCCACCCTCA CTGGCCCCAG AGCCCAAGAC CGCCAAGCTG 
GGTCCTCGGC GGGAGAGCAG CAGACCTGTG AAGCCTCCAA AGAAGGATGT ACCGGACTCA 
CAGCAGCACC CAGGGCCAGA GAAGAGCAGC AAGATCTCTG AGCAGCTAAA GTGCTGCAGT 
GGCATCCTCA AGGAGATGTT TGCCAAGAAA CATGCTGCCT ATGCCTGGCC TTTCTACAAG 
CCTGTGGATG TGGAGGCACT GGGTCTGCAC GACTACTGTG ACATCATCAA ACATCCCATG 
GACATGAGCA CAATCAAGTC TAAACTAGAG TCCCGAGAGT ACAGAGATGC CCAGGAATTT 
GGTGCTGATG TCCGATTGAT GTTCTCCAAC TGCTACAAGT ACAACCCCCC TGACCATGAA 
GTGGTAGCCA TGGCTCGAAA ACTCCAGGAT GTGTTTGAAA TGCGCTTTGC CAAGATGCCT 
GATGAGCCTG AAGAGCCAGT TGTTACAGTG TCCTCTCCTG CAGTGCCACC CCCTACAAAG 
GTGGTAGCCC CACCCTCATC TAGTGACAGC AGCAGCGACA GTTCTTCCGA CAGCGACAGT 
TCCACTGACG ACTCTGAGGA AGAGCGAGCC CAGCGGCTGG CTGAACTCCA GGAACAGCTC 
AAGGCCGTGC ATGAGCAGCT TGCAGCCCTC TCACAGCCCC AGCAGAACAA ACCAAAGAAA 
AAGGAGAAGG ACAAGAAGGA AAAGAAAAAG GAAAAGCACA AAAAGAAAGA AGAAGTGGAG 
GAAAATAAAA AAAGCAAAAC CAAGGAACTT CCTCCCAAAA AGACAAAGAA AAATAACAGC 
AGCAACAGCA ATGTGAGCAA GAAGGAACCA GTACCCACGA AGACCAAGCC GCCTCCCACA 
TATGAATCAG AAGAGGAGGA TAAGTGTAAG CCCATGTCTT ATGAGGAGAA GCGGCAGCTA 
AGTCTAGATA TCAACAAACT TCCTGGTGAG AAGCTAGGCC GTGTAGTACA CATAATTCAG 
TCAAGGGAAC CATCACTTAA AAACTCCAAC CCCGATGAGA TTGAGATTGA CTTTGAGACC 
CTGAAGCCAT CTACACTACG AGAGTTGGAG CGATATGTCA CCTCCTGTTT GCGGAAGAAA 
AGGAAACCTC AAGCTGAAAA AGTTGACGTG ATTGCTGGTT CTTCCAAGAT GAAGGGATTC 
TCATCCTCTG AGTCGGAGAG CACCAGCGAA TCCAGCTCCT CTGACAGTGA AGACTCTGAA 
ACAGAGATGG CTCCCAAGTC AAAAAAGAAG GGGCACACTG GGAGGGACCA GAAAAAGCAT 
CATCACCATC ACCATCCACA GATGCAGCCA GCCCCAGCTC CTGTGCCCCA GCAGCCGCCC 
CCACCTCCAC AGCAGCCTCC TCCACCCCCA CCTCCGCAGC AGCAGCAGCA GCAACCTCCA 
CCCCCACCAC CTCCGCCCTC CATGCCACAG CAGACTGCCC CAGCGATGAA GTCCTCGCCC 
CCACCCTTCA TCACTGCCCA GGTCCCCGTC CTGGAACCAC AGCTGCCAGG CAGTGTCTTT 
GACCCTATTG GCCACTTCAC CCAGCCCATC TTGCACCTGC CGCAGCCGGA GCTGCCTCCT 
CACCTGCCCC AGCCACCTGA GCACAGCACT CCACCCCATC TCAACCAGCA TGCTGTGGTC 
TCTCCTCCAG CTTTGCACAA TGCGCTGCCC CAACAGCCAT CTCGGCCCAG TAACCGAGCT 
GCTGCTCTGC CCCCAAAGCC TACCCGACCC CCAGCTGTGT CCCCTGCCCT GGCCCAGCCC 
CCCCTGCTCC CACAACCACC AATGGCTCAG CCCCCCCAAG TGCTGCTGGA GGATGAAGAG 
CCACCTGCCC CACCCCTCAC CTCCATGCAG ATGCAGTTGT ACCTACAGCA GCTGCAGAAG 
GTGCAGCCTC CCACACCACT ACTCCCTTCC GTGAAGGTGC AGTCCCAACC CCCACCGCCT 
TTGCCGCCTC CACCCCACCC TTCTGTGCAG CAGCAGCAGC TCCAGCCACA GCCACCGCCA 
CCCCCACCTC CTCAGCCACA GCCACCACCC CAGCAACAAC ACCAGCCTCC ACCACGACCA 
GTTCACTTGC CATCCATGCC CTTTTCTGCT CATATTCAGC AGCCCCCACC ACCCCCAGGA 
CAGCAACCTA CTCACCCACC CCCAGGACAG CAGCCCCCAC CACCACAGCC TGCCAAGCCC 
CAGCAAGTCA TCCAGCATCA CCCTTCCCCC CGGCACCACA AGTCAGACCC CTACTCAGCT 
GGTCATCTTC GTGAGGCTCC CTCTCCGCTT ATGATACATT CCCCTCAGAT GCCACAGTTC 
CAGAGCCTGA CCCATCAGTC TCCTCCCCAG CAAAACGTCC AGCCTAAGAA GCAGGTAAAG 
GGCAGGGCTG AGCCACAGCC ACCAGGGCCA GTCATGGGCC AAGGCCAGGG ATGCCCACCT 
GCCTCACCGG CTGCCGTGCC TATGCTGTCC CAGGAGCTGC GACCACCTTC AGTCGTCCAG 
CCCCAGCCCC TGGTGGTAGT AAAGGAGGAG AAGATTCACT CACCAATCAT TCGCAGCGAG 
CCTTTCAGCA CCTCACTTCG ACCAGAGCCC CCCAAGCACC CAGAGAACAT CAAGGCCCCA 
GTCCACCTGC CCCAGCGGCC TGAGATGAAG CCTGTAGACA TAGGGAGGCC TGTGATCCGG 
CCTCCAGAGC AGAGTGCACC CCCACCAGGG GCCCCTGACA AGGACAAACA GAAGCAGGAG 
CCAAAAACAC CAGTGGCACC CAAAAAGGAC CTGAAAATTA AGAACATGGG CTCCTGGGCC 
AGCCTGGTAC AGAAGCATCC GACCACCCCA TCCTCCACAG CCAAGTCCTC AAGTGACAGC 
TTTGAGCATT TCCGCCGTGC TGCTCGGGAG AAGGAGGAGA GGGAAAAGGC CCTGAAGGCT 
CAGGCTGAGC ATGCAGAGAA GGAGAAGGAG CGGCTGAGGC AGGAGCGCAT GAGGAGCCGA 
GAGGATGAAG ATGCGCTGGA ACAGGCACGT CGAGCCCATG AGGAGGCACG ACGGCGCCAG 
GAGCAGCAGC AGCAGCAGCA GCAGCAGAGA CAGGAGCAGC AGCAGCAGCA GCAGCAGGCA 
GCTGCTGTGG CCGCTGCCTC TGCCCCCCAG GCCCAGAGCT CCCAGCCCCA GTCTATGCTG 
GACCAGCAGA GGGAGTTGGC CCGGAAACGA GAGCAGGAGC GGAGGCGCAG GGAGGCAATG 
GCAGCTACAA TTGACATGAA TTTCCAGAGT GATCTTTTGT CAATATTTGA AGAAAATCTT 
TTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brd4 ( XM_006524683.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524746.1 Mus musculus bromodomain containing 4 (Brd4), transcript variant X1, mRNA.

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CloneID	OMu17143
Clone ID Related Accession (Same CDS sequence)	XM_006524683.2 , XM_006524683.3 , XM_006524684.2 , XM_030249922.1 , XM_006524686.2 , XM_006524687.2 , XM_006524685.2 , XM_030249923.1 , NM_001286630.1 , XM_006524685.1 , XM_006524686.1 , XM_006524684.3
Accession Version	XM_006524683.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4206bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	bromodomain-containing protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006524683.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGCC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGACAGACA 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCCGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAAAAA 
ATCAATGAAC TGCCTACAGA AGAAACTGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGAGGGAGGA AAGAAACAGG TAGGGCAGCA AAGCCTGGTG TATCCACGGT ACCAAACACA 
ACTCAAGCAT CAACTTCTCC GCAGACCCAG ACGCCTCAGC AGAACCCTCC TCCACCTGTG 
CAGGCCACAA CTCACCCCTT TCCTGCTGTC ACCCCAGACC TCATTGCCCA GCCTCCTGTC 
ATGACAATGG TGCCCCCTCA GCCACTTCAG ACTCCTTCAC CGGTACCCCC CCAGCCACCA 
CCCCCACCTG CTCCAGTTCC ACAGCCTGTG CAGAGTCACC CGCCCATCAT TGCGACCACC 
CCCCAGCCTG TGAAGACAAA GAAAGGGGTG AAGAGGAAAG CAGATACCAC CACCCCTACC 
ACCATCGACC CCATTCATGA GCCACCCTCA CTGGCCCCAG AGCCCAAGAC CGCCAAGCTG 
GGTCCTCGGC GGGAGAGCAG CAGACCTGTG AAGCCTCCAA AGAAGGATGT ACCGGACTCA 
CAGCAGCACC CAGGGCCAGA GAAGAGCAGC AAGATCTCTG AGCAGCTAAA GTGCTGCAGT 
GGCATCCTCA AGGAGATGTT TGCCAAGAAA CATGCTGCCT ATGCCTGGCC TTTCTACAAG 
CCTGTGGATG TGGAGGCACT GGGTCTGCAC GACTACTGTG ACATCATCAA ACATCCCATG 
GACATGAGCA CAATCAAGTC TAAACTAGAG TCCCGAGAGT ACAGAGATGC CCAGGAATTT 
GGTGCTGATG TCCGATTGAT GTTCTCCAAC TGCTACAAGT ACAACCCCCC TGACCATGAA 
GTGGTAGCCA TGGCTCGAAA ACTCCAGGAT GTGTTTGAAA TGCGCTTTGC CAAGATGCCT 
GATGAGCCTG AAGAGCCAGT TGTTACAGTG TCCTCTCCTG CAGTGCCACC CCCTACAAAG 
GTGGTAGCCC CACCCTCATC TAGTGACAGC AGCAGCGACA GTTCTTCCGA CAGCGACAGT 
TCCACTGACG ACTCTGAGGA AGAGCGAGCC CAGCGGCTGG CTGAACTCCA GGAACAGCTC 
AAGGCCGTGC ATGAGCAGCT TGCAGCCCTC TCACAGCCCC AGCAGAACAA ACCAAAGAAA 
AAGGAGAAGG ACAAGAAGGA AAAGAAAAAG GAAAAGCACA AAAAGAAAGA AGAAGTGGAG 
GAAAATAAAA AAAGCAAAAC CAAGGAACTT CCTCCCAAAA AGACAAAGAA AAATAACAGC 
AGCAACAGCA ATGTGAGCAA GAAGGAACCA GTACCCACGA AGACCAAGCC GCCTCCCACA 
TATGAATCAG AAGAGGAGGA TAAGTGTAAG CCCATGTCTT ATGAGGAGAA GCGGCAGCTA 
AGTCTAGATA TCAACAAACT TCCTGGTGAG AAGCTAGGCC GTGTAGTACA CATAATTCAG 
TCAAGGGAAC CATCACTTAA AAACTCCAAC CCCGATGAGA TTGAGATTGA CTTTGAGACC 
CTGAAGCCAT CTACACTACG AGAGTTGGAG CGATATGTCA CCTCCTGTTT GCGGAAGAAA 
AGGAAACCTC AAGCTGAAAA AGTTGACGTG ATTGCTGGTT CTTCCAAGAT GAAGGGATTC 
TCATCCTCTG AGTCGGAGAG CACCAGCGAA TCCAGCTCCT CTGACAGTGA AGACTCTGAA 
ACAGAGATGG CTCCCAAGTC AAAAAAGAAG GGGCACACTG GGAGGGACCA GAAAAAGCAT 
CATCACCATC ACCATCCACA GATGCAGCCA GCCCCAGCTC CTGTGCCCCA GCAGCCGCCC 
CCACCTCCAC AGCAGCCTCC TCCACCCCCA CCTCCGCAGC AGCAGCAGCA GCAACCTCCA 
CCCCCACCAC CTCCGCCCTC CATGCCACAG CAGACTGCCC CAGCGATGAA GTCCTCGCCC 
CCACCCTTCA TCACTGCCCA GGTCCCCGTC CTGGAACCAC AGCTGCCAGG CAGTGTCTTT 
GACCCTATTG GCCACTTCAC CCAGCCCATC TTGCACCTGC CGCAGCCGGA GCTGCCTCCT 
CACCTGCCCC AGCCACCTGA GCACAGCACT CCACCCCATC TCAACCAGCA TGCTGTGGTC 
TCTCCTCCAG CTTTGCACAA TGCGCTGCCC CAACAGCCAT CTCGGCCCAG TAACCGAGCT 
GCTGCTCTGC CCCCAAAGCC TACCCGACCC CCAGCTGTGT CCCCTGCCCT GGCCCAGCCC 
CCCCTGCTCC CACAACCACC AATGGCTCAG CCCCCCCAAG TGCTGCTGGA GGATGAAGAG 
CCACCTGCCC CACCCCTCAC CTCCATGCAG ATGCAGTTGT ACCTACAGCA GCTGCAGAAG 
GTGCAGCCTC CCACACCACT ACTCCCTTCC GTGAAGGTGC AGTCCCAACC CCCACCGCCT 
TTGCCGCCTC CACCCCACCC TTCTGTGCAG CAGCAGCAGC TCCAGCCACA GCCACCGCCA 
CCCCCACCTC CTCAGCCACA GCCACCACCC CAGCAACAAC ACCAGCCTCC ACCACGACCA 
GTTCACTTGC CATCCATGCC CTTTTCTGCT CATATTCAGC AGCCCCCACC ACCCCCAGGA 
CAGCAACCTA CTCACCCACC CCCAGGACAG CAGCCCCCAC CACCACAGCC TGCCAAGCCC 
CAGCAAGTCA TCCAGCATCA CCCTTCCCCC CGGCACCACA AGTCAGACCC CTACTCAGCT 
GGTCATCTTC GTGAGGCTCC CTCTCCGCTT ATGATACATT CCCCTCAGAT GCCACAGTTC 
CAGAGCCTGA CCCATCAGTC TCCTCCCCAG CAAAACGTCC AGCCTAAGAA GCAGGTAAAG 
GGCAGGGCTG AGCCACAGCC ACCAGGGCCA GTCATGGGCC AAGGCCAGGG ATGCCCACCT 
GCCTCACCGG CTGCCGTGCC TATGCTGTCC CAGGAGCTGC GACCACCTTC AGTCGTCCAG 
CCCCAGCCCC TGGTGGTAGT AAAGGAGGAG AAGATTCACT CACCAATCAT TCGCAGCGAG 
CCTTTCAGCA CCTCACTTCG ACCAGAGCCC CCCAAGCACC CAGAGAACAT CAAGGCCCCA 
GTCCACCTGC CCCAGCGGCC TGAGATGAAG CCTGTAGACA TAGGGAGGCC TGTGATCCGG 
CCTCCAGAGC AGAGTGCACC CCCACCAGGG GCCCCTGACA AGGACAAACA GAAGCAGGAG 
CCAAAAACAC CAGTGGCACC CAAAAAGGAC CTGAAAATTA AGAACATGGG CTCCTGGGCC 
AGCCTGGTAC AGAAGCATCC GACCACCCCA TCCTCCACAG CCAAGTCCTC AAGTGACAGC 
TTTGAGCATT TCCGCCGTGC TGCTCGGGAG AAGGAGGAGA GGGAAAAGGC CCTGAAGGCT 
CAGGCTGAGC ATGCAGAGAA GGAGAAGGAG CGGCTGAGGC AGGAGCGCAT GAGGAGCCGA 
GAGGATGAAG ATGCGCTGGA ACAGGCACGT CGAGCCCATG AGGAGGCACG ACGGCGCCAG 
GAGCAGCAGC AGCAGCAGCA GCAGCAGAGA CAGGAGCAGC AGCAGCAGCA GCAGCAGGCA 
GCTGCTGTGG CCGCTGCCTC TGCCCCCCAG GCCCAGAGCT CCCAGCCCCA GTCTATGCTG 
GACCAGCAGA GGGAGTTGGC CCGGAAACGA GAGCAGGAGC GGAGGCGCAG GGAGGCAATG 
GCAGCTACAA TTGACATGAA TTTCCAGAGT GATCTTTTGT CAATATTTGA AGAAAATCTT 
TTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524746.1
CDS	261..4466
Translation	MSTESGPGTRLRNLPVMGDGLETSQMSTTQAQAQPQPANAASTNPPPPETSNPNKPKRQTNQLQYLLRVVLKTLWKHQFAWPFQQPVDAVKLNLPDYYKIIKTPMDMGTIKKRLENNYYWNAQECIQDFNTMFTNCYIYNKPGDDIVLMAEALEKLFLQKINELPTEETEIMIVQAKGRGRGRKETGRAAKPGVSTVPNTTQASTSPQTQTPQQNPPPPVQATTHPFPAVTPDLIAQPPVMTMVPPQPLQTPSPVPPQPPPPPAPVPQPVQSHPPIIATTPQPVKTKKGVKRKADTTTPTTIDPIHEPPSLAPEPKTAKLGPRRESSRPVKPPKKDVPDSQQHPGPEKSSKISEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYCDIIKHPMDMSTIKSKLESREYRDAQEFGADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPEEPVVTVSSPAVPPPTKVVAPPSSSDSSSDSSSDSDSSTDDSEEERAQRLAELQEQLKAVHEQLAALSQPQQNKPKKKEKDKKEKKKEKHKKKEEVEENKKSKTKELPPKKTKKNNSSNSNVSKKEPVPTKTKPPPTYESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVDVIAGSSKMKGFSSSESESTSESSSSDSEDSETEMAPKSKKKGHTGRDQKKHHHHHHPQMQPAPAPVPQQPPPPPQQPPPPPPPQQQQQQPPPPPPPPSMPQQTAPAMKSSPPPFITAQVPVLEPQLPGSVFDPIGHFTQPILHLPQPELPPHLPQPPEHSTPPHLNQHAVVSPPALHNALPQQPSRPSNRAAALPPKPTRPPAVSPALAQPPLLPQPPMAQPPQVLLEDEEPPAPPLTSMQMQLYLQQLQKVQPPTPLLPSVKVQSQPPPPLPPPPHPSVQQQQLQPQPPPPPPPQPQPPPQQQHQPPPRPVHLPSMPFSAHIQQPPPPPGQQPTHPPPGQQPPPPQPAKPQQVIQHHPSPRHHKSDPYSAGHLREAPSPLMIHSPQMPQFQSLTHQSPPQQNVQPKKQVKGRAEPQPPGPVMGQGQGCPPASPAAVPMLSQELRPPSVVQPQPLVVVKEEKIHSPIIRSEPFSTSLRPEPPKHPENIKAPVHLPQRPEMKPVDIGRPVIRPPEQSAPPPGAPDKDKQKQEPKTPVAPKKDLKIKNMGSWASLVQKHPTTPSSTAKSSSDSFEHFRRAAREKEEREKALKAQAEHAEKEKERLRQERMRSREDEDALEQARRAHEEARRRQEQQQQQQQQRQEQQQQQQQAAAVAAASAPQAQSSQPQSMLDQQRELARKREQERRRREAMAATIDMNFQSDLLSIFEENLF

Target ORF information:

RefSeq Version	XM_006524683.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus bromodomain containing 4 (Brd4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524683.3

ORF Insert Sequence:

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGCC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGACAGACA 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCCGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAAAAA 
ATCAATGAAC TGCCTACAGA AGAAACTGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGAGGGAGGA AAGAAACAGG TAGGGCAGCA AAGCCTGGTG TATCCACGGT ACCAAACACA 
ACTCAAGCAT CAACTTCTCC GCAGACCCAG ACGCCTCAGC AGAACCCTCC TCCACCTGTG 
CAGGCCACAA CTCACCCCTT TCCTGCTGTC ACCCCAGACC TCATTGCCCA GCCTCCTGTC 
ATGACAATGG TGCCCCCTCA GCCACTTCAG ACTCCTTCAC CGGTACCCCC CCAGCCACCA 
CCCCCACCTG CTCCAGTTCC ACAGCCTGTG CAGAGTCACC CGCCCATCAT TGCGACCACC 
CCCCAGCCTG TGAAGACAAA GAAAGGGGTG AAGAGGAAAG CAGATACCAC CACCCCTACC 
ACCATCGACC CCATTCATGA GCCACCCTCA CTGGCCCCAG AGCCCAAGAC CGCCAAGCTG 
GGTCCTCGGC GGGAGAGCAG CAGACCTGTG AAGCCTCCAA AGAAGGATGT ACCGGACTCA 
CAGCAGCACC CAGGGCCAGA GAAGAGCAGC AAGATCTCTG AGCAGCTAAA GTGCTGCAGT 
GGCATCCTCA AGGAGATGTT TGCCAAGAAA CATGCTGCCT ATGCCTGGCC TTTCTACAAG 
CCTGTGGATG TGGAGGCACT GGGTCTGCAC GACTACTGTG ACATCATCAA ACATCCCATG 
GACATGAGCA CAATCAAGTC TAAACTAGAG TCCCGAGAGT ACAGAGATGC CCAGGAATTT 
GGTGCTGATG TCCGATTGAT GTTCTCCAAC TGCTACAAGT ACAACCCCCC TGACCATGAA 
GTGGTAGCCA TGGCTCGAAA ACTCCAGGAT GTGTTTGAAA TGCGCTTTGC CAAGATGCCT 
GATGAGCCTG AAGAGCCAGT TGTTACAGTG TCCTCTCCTG CAGTGCCACC CCCTACAAAG 
GTGGTAGCCC CACCCTCATC TAGTGACAGC AGCAGCGACA GTTCTTCCGA CAGCGACAGT 
TCCACTGACG ACTCTGAGGA AGAGCGAGCC CAGCGGCTGG CTGAACTCCA GGAACAGCTC 
AAGGCCGTGC ATGAGCAGCT TGCAGCCCTC TCACAGCCCC AGCAGAACAA ACCAAAGAAA 
AAGGAGAAGG ACAAGAAGGA AAAGAAAAAG GAAAAGCACA AAAAGAAAGA AGAAGTGGAG 
GAAAATAAAA AAAGCAAAAC CAAGGAACTT CCTCCCAAAA AGACAAAGAA AAATAACAGC 
AGCAACAGCA ATGTGAGCAA GAAGGAACCA GTACCCACGA AGACCAAGCC GCCTCCCACA 
TATGAATCAG AAGAGGAGGA TAAGTGTAAG CCCATGTCTT ATGAGGAGAA GCGGCAGCTA 
AGTCTAGATA TCAACAAACT TCCTGGTGAG AAGCTAGGCC GTGTAGTACA CATAATTCAG 
TCAAGGGAAC CATCACTTAA AAACTCCAAC CCCGATGAGA TTGAGATTGA CTTTGAGACC 
CTGAAGCCAT CTACACTACG AGAGTTGGAG CGATATGTCA CCTCCTGTTT GCGGAAGAAA 
AGGAAACCTC AAGCTGAAAA AGTTGACGTG ATTGCTGGTT CTTCCAAGAT GAAGGGATTC 
TCATCCTCTG AGTCGGAGAG CACCAGCGAA TCCAGCTCCT CTGACAGTGA AGACTCTGAA 
ACAGAGATGG CTCCCAAGTC AAAAAAGAAG GGGCACACTG GGAGGGACCA GAAAAAGCAT 
CATCACCATC ACCATCCACA GATGCAGCCA GCCCCAGCTC CTGTGCCCCA GCAGCCGCCC 
CCACCTCCAC AGCAGCCTCC TCCACCCCCA CCTCCGCAGC AGCAGCAGCA GCAACCTCCA 
CCCCCACCAC CTCCGCCCTC CATGCCACAG CAGACTGCCC CAGCGATGAA GTCCTCGCCC 
CCACCCTTCA TCACTGCCCA GGTCCCCGTC CTGGAACCAC AGCTGCCAGG CAGTGTCTTT 
GACCCTATTG GCCACTTCAC CCAGCCCATC TTGCACCTGC CGCAGCCGGA GCTGCCTCCT 
CACCTGCCCC AGCCACCTGA GCACAGCACT CCACCCCATC TCAACCAGCA TGCTGTGGTC 
TCTCCTCCAG CTTTGCACAA TGCGCTGCCC CAACAGCCAT CTCGGCCCAG TAACCGAGCT 
GCTGCTCTGC CCCCAAAGCC TACCCGACCC CCAGCTGTGT CCCCTGCCCT GGCCCAGCCC 
CCCCTGCTCC CACAACCACC AATGGCTCAG CCCCCCCAAG TGCTGCTGGA GGATGAAGAG 
CCACCTGCCC CACCCCTCAC CTCCATGCAG ATGCAGTTGT ACCTACAGCA GCTGCAGAAG 
GTGCAGCCTC CCACACCACT ACTCCCTTCC GTGAAGGTGC AGTCCCAACC CCCACCGCCT 
TTGCCGCCTC CACCCCACCC TTCTGTGCAG CAGCAGCAGC TCCAGCCACA GCCACCGCCA 
CCCCCACCTC CTCAGCCACA GCCACCACCC CAGCAACAAC ACCAGCCTCC ACCACGACCA 
GTTCACTTGC CATCCATGCC CTTTTCTGCT CATATTCAGC AGCCCCCACC ACCCCCAGGA 
CAGCAACCTA CTCACCCACC CCCAGGACAG CAGCCCCCAC CACCACAGCC TGCCAAGCCC 
CAGCAAGTCA TCCAGCATCA CCCTTCCCCC CGGCACCACA AGTCAGACCC CTACTCAGCT 
GGTCATCTTC GTGAGGCTCC CTCTCCGCTT ATGATACATT CCCCTCAGAT GCCACAGTTC 
CAGAGCCTGA CCCATCAGTC TCCTCCCCAG CAAAACGTCC AGCCTAAGAA GCAGGTAAAG 
GGCAGGGCTG AGCCACAGCC ACCAGGGCCA GTCATGGGCC AAGGCCAGGG ATGCCCACCT 
GCCTCACCGG CTGCCGTGCC TATGCTGTCC CAGGAGCTGC GACCACCTTC AGTCGTCCAG 
CCCCAGCCCC TGGTGGTAGT AAAGGAGGAG AAGATTCACT CACCAATCAT TCGCAGCGAG 
CCTTTCAGCA CCTCACTTCG ACCAGAGCCC CCCAAGCACC CAGAGAACAT CAAGGCCCCA 
GTCCACCTGC CCCAGCGGCC TGAGATGAAG CCTGTAGACA TAGGGAGGCC TGTGATCCGG 
CCTCCAGAGC AGAGTGCACC CCCACCAGGG GCCCCTGACA AGGACAAACA GAAGCAGGAG 
CCAAAAACAC CAGTGGCACC CAAAAAGGAC CTGAAAATTA AGAACATGGG CTCCTGGGCC 
AGCCTGGTAC AGAAGCATCC GACCACCCCA TCCTCCACAG CCAAGTCCTC AAGTGACAGC 
TTTGAGCATT TCCGCCGTGC TGCTCGGGAG AAGGAGGAGA GGGAAAAGGC CCTGAAGGCT 
CAGGCTGAGC ATGCAGAGAA GGAGAAGGAG CGGCTGAGGC AGGAGCGCAT GAGGAGCCGA 
GAGGATGAAG ATGCGCTGGA ACAGGCACGT CGAGCCCATG AGGAGGCACG ACGGCGCCAG 
GAGCAGCAGC AGCAGCAGCA GCAGCAGAGA CAGGAGCAGC AGCAGCAGCA GCAGCAGGCA 
GCTGCTGTGG CCGCTGCCTC TGCCCCCCAG GCCCAGAGCT CCCAGCCCCA GTCTATGCTG 
GACCAGCAGA GGGAGTTGGC CCGGAAACGA GAGCAGGAGC GGAGGCGCAG GGAGGCAATG 
GCAGCTACAA TTGACATGAA TTTCCAGAGT GATCTTTTGT CAATATTTGA AGAAAATCTT 
TTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brd4 ( XM_006524684.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524747.1 Mus musculus bromodomain containing 4 (Brd4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu17143
Clone ID Related Accession (Same CDS sequence)	XM_006524683.2 , XM_006524683.3 , XM_006524684.2 , XM_030249922.1 , XM_006524686.2 , XM_006524687.2 , XM_006524685.2 , XM_030249923.1 , NM_001286630.1 , XM_006524685.1 , XM_006524686.1 , XM_006524684.3
Accession Version	XM_006524684.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4206bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	bromodomain-containing protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006524684.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGCC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGACAGACA 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCCGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAAAAA 
ATCAATGAAC TGCCTACAGA AGAAACTGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGAGGGAGGA AAGAAACAGG TAGGGCAGCA AAGCCTGGTG TATCCACGGT ACCAAACACA 
ACTCAAGCAT CAACTTCTCC GCAGACCCAG ACGCCTCAGC AGAACCCTCC TCCACCTGTG 
CAGGCCACAA CTCACCCCTT TCCTGCTGTC ACCCCAGACC TCATTGCCCA GCCTCCTGTC 
ATGACAATGG TGCCCCCTCA GCCACTTCAG ACTCCTTCAC CGGTACCCCC CCAGCCACCA 
CCCCCACCTG CTCCAGTTCC ACAGCCTGTG CAGAGTCACC CGCCCATCAT TGCGACCACC 
CCCCAGCCTG TGAAGACAAA GAAAGGGGTG AAGAGGAAAG CAGATACCAC CACCCCTACC 
ACCATCGACC CCATTCATGA GCCACCCTCA CTGGCCCCAG AGCCCAAGAC CGCCAAGCTG 
GGTCCTCGGC GGGAGAGCAG CAGACCTGTG AAGCCTCCAA AGAAGGATGT ACCGGACTCA 
CAGCAGCACC CAGGGCCAGA GAAGAGCAGC AAGATCTCTG AGCAGCTAAA GTGCTGCAGT 
GGCATCCTCA AGGAGATGTT TGCCAAGAAA CATGCTGCCT ATGCCTGGCC TTTCTACAAG 
CCTGTGGATG TGGAGGCACT GGGTCTGCAC GACTACTGTG ACATCATCAA ACATCCCATG 
GACATGAGCA CAATCAAGTC TAAACTAGAG TCCCGAGAGT ACAGAGATGC CCAGGAATTT 
GGTGCTGATG TCCGATTGAT GTTCTCCAAC TGCTACAAGT ACAACCCCCC TGACCATGAA 
GTGGTAGCCA TGGCTCGAAA ACTCCAGGAT GTGTTTGAAA TGCGCTTTGC CAAGATGCCT 
GATGAGCCTG AAGAGCCAGT TGTTACAGTG TCCTCTCCTG CAGTGCCACC CCCTACAAAG 
GTGGTAGCCC CACCCTCATC TAGTGACAGC AGCAGCGACA GTTCTTCCGA CAGCGACAGT 
TCCACTGACG ACTCTGAGGA AGAGCGAGCC CAGCGGCTGG CTGAACTCCA GGAACAGCTC 
AAGGCCGTGC ATGAGCAGCT TGCAGCCCTC TCACAGCCCC AGCAGAACAA ACCAAAGAAA 
AAGGAGAAGG ACAAGAAGGA AAAGAAAAAG GAAAAGCACA AAAAGAAAGA AGAAGTGGAG 
GAAAATAAAA AAAGCAAAAC CAAGGAACTT CCTCCCAAAA AGACAAAGAA AAATAACAGC 
AGCAACAGCA ATGTGAGCAA GAAGGAACCA GTACCCACGA AGACCAAGCC GCCTCCCACA 
TATGAATCAG AAGAGGAGGA TAAGTGTAAG CCCATGTCTT ATGAGGAGAA GCGGCAGCTA 
AGTCTAGATA TCAACAAACT TCCTGGTGAG AAGCTAGGCC GTGTAGTACA CATAATTCAG 
TCAAGGGAAC CATCACTTAA AAACTCCAAC CCCGATGAGA TTGAGATTGA CTTTGAGACC 
CTGAAGCCAT CTACACTACG AGAGTTGGAG CGATATGTCA CCTCCTGTTT GCGGAAGAAA 
AGGAAACCTC AAGCTGAAAA AGTTGACGTG ATTGCTGGTT CTTCCAAGAT GAAGGGATTC 
TCATCCTCTG AGTCGGAGAG CACCAGCGAA TCCAGCTCCT CTGACAGTGA AGACTCTGAA 
ACAGAGATGG CTCCCAAGTC AAAAAAGAAG GGGCACACTG GGAGGGACCA GAAAAAGCAT 
CATCACCATC ACCATCCACA GATGCAGCCA GCCCCAGCTC CTGTGCCCCA GCAGCCGCCC 
CCACCTCCAC AGCAGCCTCC TCCACCCCCA CCTCCGCAGC AGCAGCAGCA GCAACCTCCA 
CCCCCACCAC CTCCGCCCTC CATGCCACAG CAGACTGCCC CAGCGATGAA GTCCTCGCCC 
CCACCCTTCA TCACTGCCCA GGTCCCCGTC CTGGAACCAC AGCTGCCAGG CAGTGTCTTT 
GACCCTATTG GCCACTTCAC CCAGCCCATC TTGCACCTGC CGCAGCCGGA GCTGCCTCCT 
CACCTGCCCC AGCCACCTGA GCACAGCACT CCACCCCATC TCAACCAGCA TGCTGTGGTC 
TCTCCTCCAG CTTTGCACAA TGCGCTGCCC CAACAGCCAT CTCGGCCCAG TAACCGAGCT 
GCTGCTCTGC CCCCAAAGCC TACCCGACCC CCAGCTGTGT CCCCTGCCCT GGCCCAGCCC 
CCCCTGCTCC CACAACCACC AATGGCTCAG CCCCCCCAAG TGCTGCTGGA GGATGAAGAG 
CCACCTGCCC CACCCCTCAC CTCCATGCAG ATGCAGTTGT ACCTACAGCA GCTGCAGAAG 
GTGCAGCCTC CCACACCACT ACTCCCTTCC GTGAAGGTGC AGTCCCAACC CCCACCGCCT 
TTGCCGCCTC CACCCCACCC TTCTGTGCAG CAGCAGCAGC TCCAGCCACA GCCACCGCCA 
CCCCCACCTC CTCAGCCACA GCCACCACCC CAGCAACAAC ACCAGCCTCC ACCACGACCA 
GTTCACTTGC CATCCATGCC CTTTTCTGCT CATATTCAGC AGCCCCCACC ACCCCCAGGA 
CAGCAACCTA CTCACCCACC CCCAGGACAG CAGCCCCCAC CACCACAGCC TGCCAAGCCC 
CAGCAAGTCA TCCAGCATCA CCCTTCCCCC CGGCACCACA AGTCAGACCC CTACTCAGCT 
GGTCATCTTC GTGAGGCTCC CTCTCCGCTT ATGATACATT CCCCTCAGAT GCCACAGTTC 
CAGAGCCTGA CCCATCAGTC TCCTCCCCAG CAAAACGTCC AGCCTAAGAA GCAGGTAAAG 
GGCAGGGCTG AGCCACAGCC ACCAGGGCCA GTCATGGGCC AAGGCCAGGG ATGCCCACCT 
GCCTCACCGG CTGCCGTGCC TATGCTGTCC CAGGAGCTGC GACCACCTTC AGTCGTCCAG 
CCCCAGCCCC TGGTGGTAGT AAAGGAGGAG AAGATTCACT CACCAATCAT TCGCAGCGAG 
CCTTTCAGCA CCTCACTTCG ACCAGAGCCC CCCAAGCACC CAGAGAACAT CAAGGCCCCA 
GTCCACCTGC CCCAGCGGCC TGAGATGAAG CCTGTAGACA TAGGGAGGCC TGTGATCCGG 
CCTCCAGAGC AGAGTGCACC CCCACCAGGG GCCCCTGACA AGGACAAACA GAAGCAGGAG 
CCAAAAACAC CAGTGGCACC CAAAAAGGAC CTGAAAATTA AGAACATGGG CTCCTGGGCC 
AGCCTGGTAC AGAAGCATCC GACCACCCCA TCCTCCACAG CCAAGTCCTC AAGTGACAGC 
TTTGAGCATT TCCGCCGTGC TGCTCGGGAG AAGGAGGAGA GGGAAAAGGC CCTGAAGGCT 
CAGGCTGAGC ATGCAGAGAA GGAGAAGGAG CGGCTGAGGC AGGAGCGCAT GAGGAGCCGA 
GAGGATGAAG ATGCGCTGGA ACAGGCACGT CGAGCCCATG AGGAGGCACG ACGGCGCCAG 
GAGCAGCAGC AGCAGCAGCA GCAGCAGAGA CAGGAGCAGC AGCAGCAGCA GCAGCAGGCA 
GCTGCTGTGG CCGCTGCCTC TGCCCCCCAG GCCCAGAGCT CCCAGCCCCA GTCTATGCTG 
GACCAGCAGA GGGAGTTGGC CCGGAAACGA GAGCAGGAGC GGAGGCGCAG GGAGGCAATG 
GCAGCTACAA TTGACATGAA TTTCCAGAGT GATCTTTTGT CAATATTTGA AGAAAATCTT 
TTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524747.1
CDS	264..4469
Translation	MSTESGPGTRLRNLPVMGDGLETSQMSTTQAQAQPQPANAASTNPPPPETSNPNKPKRQTNQLQYLLRVVLKTLWKHQFAWPFQQPVDAVKLNLPDYYKIIKTPMDMGTIKKRLENNYYWNAQECIQDFNTMFTNCYIYNKPGDDIVLMAEALEKLFLQKINELPTEETEIMIVQAKGRGRGRKETGRAAKPGVSTVPNTTQASTSPQTQTPQQNPPPPVQATTHPFPAVTPDLIAQPPVMTMVPPQPLQTPSPVPPQPPPPPAPVPQPVQSHPPIIATTPQPVKTKKGVKRKADTTTPTTIDPIHEPPSLAPEPKTAKLGPRRESSRPVKPPKKDVPDSQQHPGPEKSSKISEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYCDIIKHPMDMSTIKSKLESREYRDAQEFGADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPEEPVVTVSSPAVPPPTKVVAPPSSSDSSSDSSSDSDSSTDDSEEERAQRLAELQEQLKAVHEQLAALSQPQQNKPKKKEKDKKEKKKEKHKKKEEVEENKKSKTKELPPKKTKKNNSSNSNVSKKEPVPTKTKPPPTYESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVDVIAGSSKMKGFSSSESESTSESSSSDSEDSETEMAPKSKKKGHTGRDQKKHHHHHHPQMQPAPAPVPQQPPPPPQQPPPPPPPQQQQQQPPPPPPPPSMPQQTAPAMKSSPPPFITAQVPVLEPQLPGSVFDPIGHFTQPILHLPQPELPPHLPQPPEHSTPPHLNQHAVVSPPALHNALPQQPSRPSNRAAALPPKPTRPPAVSPALAQPPLLPQPPMAQPPQVLLEDEEPPAPPLTSMQMQLYLQQLQKVQPPTPLLPSVKVQSQPPPPLPPPPHPSVQQQQLQPQPPPPPPPQPQPPPQQQHQPPPRPVHLPSMPFSAHIQQPPPPPGQQPTHPPPGQQPPPPQPAKPQQVIQHHPSPRHHKSDPYSAGHLREAPSPLMIHSPQMPQFQSLTHQSPPQQNVQPKKQVKGRAEPQPPGPVMGQGQGCPPASPAAVPMLSQELRPPSVVQPQPLVVVKEEKIHSPIIRSEPFSTSLRPEPPKHPENIKAPVHLPQRPEMKPVDIGRPVIRPPEQSAPPPGAPDKDKQKQEPKTPVAPKKDLKIKNMGSWASLVQKHPTTPSSTAKSSSDSFEHFRRAAREKEEREKALKAQAEHAEKEKERLRQERMRSREDEDALEQARRAHEEARRRQEQQQQQQQQRQEQQQQQQQAAAVAAASAPQAQSSQPQSMLDQQRELARKREQERRRREAMAATIDMNFQSDLLSIFEENLF

Target ORF information:

RefSeq Version	XM_006524684.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus bromodomain containing 4 (Brd4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524684.2

ORF Insert Sequence:

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGCC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGACAGACA 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCCGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAAAAA 
ATCAATGAAC TGCCTACAGA AGAAACTGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGAGGGAGGA AAGAAACAGG TAGGGCAGCA AAGCCTGGTG TATCCACGGT ACCAAACACA 
ACTCAAGCAT CAACTTCTCC GCAGACCCAG ACGCCTCAGC AGAACCCTCC TCCACCTGTG 
CAGGCCACAA CTCACCCCTT TCCTGCTGTC ACCCCAGACC TCATTGCCCA GCCTCCTGTC 
ATGACAATGG TGCCCCCTCA GCCACTTCAG ACTCCTTCAC CGGTACCCCC CCAGCCACCA 
CCCCCACCTG CTCCAGTTCC ACAGCCTGTG CAGAGTCACC CGCCCATCAT TGCGACCACC 
CCCCAGCCTG TGAAGACAAA GAAAGGGGTG AAGAGGAAAG CAGATACCAC CACCCCTACC 
ACCATCGACC CCATTCATGA GCCACCCTCA CTGGCCCCAG AGCCCAAGAC CGCCAAGCTG 
GGTCCTCGGC GGGAGAGCAG CAGACCTGTG AAGCCTCCAA AGAAGGATGT ACCGGACTCA 
CAGCAGCACC CAGGGCCAGA GAAGAGCAGC AAGATCTCTG AGCAGCTAAA GTGCTGCAGT 
GGCATCCTCA AGGAGATGTT TGCCAAGAAA CATGCTGCCT ATGCCTGGCC TTTCTACAAG 
CCTGTGGATG TGGAGGCACT GGGTCTGCAC GACTACTGTG ACATCATCAA ACATCCCATG 
GACATGAGCA CAATCAAGTC TAAACTAGAG TCCCGAGAGT ACAGAGATGC CCAGGAATTT 
GGTGCTGATG TCCGATTGAT GTTCTCCAAC TGCTACAAGT ACAACCCCCC TGACCATGAA 
GTGGTAGCCA TGGCTCGAAA ACTCCAGGAT GTGTTTGAAA TGCGCTTTGC CAAGATGCCT 
GATGAGCCTG AAGAGCCAGT TGTTACAGTG TCCTCTCCTG CAGTGCCACC CCCTACAAAG 
GTGGTAGCCC CACCCTCATC TAGTGACAGC AGCAGCGACA GTTCTTCCGA CAGCGACAGT 
TCCACTGACG ACTCTGAGGA AGAGCGAGCC CAGCGGCTGG CTGAACTCCA GGAACAGCTC 
AAGGCCGTGC ATGAGCAGCT TGCAGCCCTC TCACAGCCCC AGCAGAACAA ACCAAAGAAA 
AAGGAGAAGG ACAAGAAGGA AAAGAAAAAG GAAAAGCACA AAAAGAAAGA AGAAGTGGAG 
GAAAATAAAA AAAGCAAAAC CAAGGAACTT CCTCCCAAAA AGACAAAGAA AAATAACAGC 
AGCAACAGCA ATGTGAGCAA GAAGGAACCA GTACCCACGA AGACCAAGCC GCCTCCCACA 
TATGAATCAG AAGAGGAGGA TAAGTGTAAG CCCATGTCTT ATGAGGAGAA GCGGCAGCTA 
AGTCTAGATA TCAACAAACT TCCTGGTGAG AAGCTAGGCC GTGTAGTACA CATAATTCAG 
TCAAGGGAAC CATCACTTAA AAACTCCAAC CCCGATGAGA TTGAGATTGA CTTTGAGACC 
CTGAAGCCAT CTACACTACG AGAGTTGGAG CGATATGTCA CCTCCTGTTT GCGGAAGAAA 
AGGAAACCTC AAGCTGAAAA AGTTGACGTG ATTGCTGGTT CTTCCAAGAT GAAGGGATTC 
TCATCCTCTG AGTCGGAGAG CACCAGCGAA TCCAGCTCCT CTGACAGTGA AGACTCTGAA 
ACAGAGATGG CTCCCAAGTC AAAAAAGAAG GGGCACACTG GGAGGGACCA GAAAAAGCAT 
CATCACCATC ACCATCCACA GATGCAGCCA GCCCCAGCTC CTGTGCCCCA GCAGCCGCCC 
CCACCTCCAC AGCAGCCTCC TCCACCCCCA CCTCCGCAGC AGCAGCAGCA GCAACCTCCA 
CCCCCACCAC CTCCGCCCTC CATGCCACAG CAGACTGCCC CAGCGATGAA GTCCTCGCCC 
CCACCCTTCA TCACTGCCCA GGTCCCCGTC CTGGAACCAC AGCTGCCAGG CAGTGTCTTT 
GACCCTATTG GCCACTTCAC CCAGCCCATC TTGCACCTGC CGCAGCCGGA GCTGCCTCCT 
CACCTGCCCC AGCCACCTGA GCACAGCACT CCACCCCATC TCAACCAGCA TGCTGTGGTC 
TCTCCTCCAG CTTTGCACAA TGCGCTGCCC CAACAGCCAT CTCGGCCCAG TAACCGAGCT 
GCTGCTCTGC CCCCAAAGCC TACCCGACCC CCAGCTGTGT CCCCTGCCCT GGCCCAGCCC 
CCCCTGCTCC CACAACCACC AATGGCTCAG CCCCCCCAAG TGCTGCTGGA GGATGAAGAG 
CCACCTGCCC CACCCCTCAC CTCCATGCAG ATGCAGTTGT ACCTACAGCA GCTGCAGAAG 
GTGCAGCCTC CCACACCACT ACTCCCTTCC GTGAAGGTGC AGTCCCAACC CCCACCGCCT 
TTGCCGCCTC CACCCCACCC TTCTGTGCAG CAGCAGCAGC TCCAGCCACA GCCACCGCCA 
CCCCCACCTC CTCAGCCACA GCCACCACCC CAGCAACAAC ACCAGCCTCC ACCACGACCA 
GTTCACTTGC CATCCATGCC CTTTTCTGCT CATATTCAGC AGCCCCCACC ACCCCCAGGA 
CAGCAACCTA CTCACCCACC CCCAGGACAG CAGCCCCCAC CACCACAGCC TGCCAAGCCC 
CAGCAAGTCA TCCAGCATCA CCCTTCCCCC CGGCACCACA AGTCAGACCC CTACTCAGCT 
GGTCATCTTC GTGAGGCTCC CTCTCCGCTT ATGATACATT CCCCTCAGAT GCCACAGTTC 
CAGAGCCTGA CCCATCAGTC TCCTCCCCAG CAAAACGTCC AGCCTAAGAA GCAGGTAAAG 
GGCAGGGCTG AGCCACAGCC ACCAGGGCCA GTCATGGGCC AAGGCCAGGG ATGCCCACCT 
GCCTCACCGG CTGCCGTGCC TATGCTGTCC CAGGAGCTGC GACCACCTTC AGTCGTCCAG 
CCCCAGCCCC TGGTGGTAGT AAAGGAGGAG AAGATTCACT CACCAATCAT TCGCAGCGAG 
CCTTTCAGCA CCTCACTTCG ACCAGAGCCC CCCAAGCACC CAGAGAACAT CAAGGCCCCA 
GTCCACCTGC CCCAGCGGCC TGAGATGAAG CCTGTAGACA TAGGGAGGCC TGTGATCCGG 
CCTCCAGAGC AGAGTGCACC CCCACCAGGG GCCCCTGACA AGGACAAACA GAAGCAGGAG 
CCAAAAACAC CAGTGGCACC CAAAAAGGAC CTGAAAATTA AGAACATGGG CTCCTGGGCC 
AGCCTGGTAC AGAAGCATCC GACCACCCCA TCCTCCACAG CCAAGTCCTC AAGTGACAGC 
TTTGAGCATT TCCGCCGTGC TGCTCGGGAG AAGGAGGAGA GGGAAAAGGC CCTGAAGGCT 
CAGGCTGAGC ATGCAGAGAA GGAGAAGGAG CGGCTGAGGC AGGAGCGCAT GAGGAGCCGA 
GAGGATGAAG ATGCGCTGGA ACAGGCACGT CGAGCCCATG AGGAGGCACG ACGGCGCCAG 
GAGCAGCAGC AGCAGCAGCA GCAGCAGAGA CAGGAGCAGC AGCAGCAGCA GCAGCAGGCA 
GCTGCTGTGG CCGCTGCCTC TGCCCCCCAG GCCCAGAGCT CCCAGCCCCA GTCTATGCTG 
GACCAGCAGA GGGAGTTGGC CCGGAAACGA GAGCAGGAGC GGAGGCGCAG GGAGGCAATG 
GCAGCTACAA TTGACATGAA TTTCCAGAGT GATCTTTTGT CAATATTTGA AGAAAATCTT 
TTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brd4 ( XM_030249922.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105782.1 Mus musculus bromodomain containing 4 (Brd4), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu17143
Clone ID Related Accession (Same CDS sequence)	XM_006524683.2 , XM_006524683.3 , XM_006524684.2 , XM_030249922.1 , XM_006524686.2 , XM_006524687.2 , XM_006524685.2 , XM_030249923.1 , NM_001286630.1 , XM_006524685.1 , XM_006524686.1 , XM_006524684.3
Accession Version	XM_030249922.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4206bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	bromodomain-containing protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGCC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGACAGACA 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCCGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAAAAA 
ATCAATGAAC TGCCTACAGA AGAAACTGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGAGGGAGGA AAGAAACAGG TAGGGCAGCA AAGCCTGGTG TATCCACGGT ACCAAACACA 
ACTCAAGCAT CAACTTCTCC GCAGACCCAG ACGCCTCAGC AGAACCCTCC TCCACCTGTG 
CAGGCCACAA CTCACCCCTT TCCTGCTGTC ACCCCAGACC TCATTGCCCA GCCTCCTGTC 
ATGACAATGG TGCCCCCTCA GCCACTTCAG ACTCCTTCAC CGGTACCCCC CCAGCCACCA 
CCCCCACCTG CTCCAGTTCC ACAGCCTGTG CAGAGTCACC CGCCCATCAT TGCGACCACC 
CCCCAGCCTG TGAAGACAAA GAAAGGGGTG AAGAGGAAAG CAGATACCAC CACCCCTACC 
ACCATCGACC CCATTCATGA GCCACCCTCA CTGGCCCCAG AGCCCAAGAC CGCCAAGCTG 
GGTCCTCGGC GGGAGAGCAG CAGACCTGTG AAGCCTCCAA AGAAGGATGT ACCGGACTCA 
CAGCAGCACC CAGGGCCAGA GAAGAGCAGC AAGATCTCTG AGCAGCTAAA GTGCTGCAGT 
GGCATCCTCA AGGAGATGTT TGCCAAGAAA CATGCTGCCT ATGCCTGGCC TTTCTACAAG 
CCTGTGGATG TGGAGGCACT GGGTCTGCAC GACTACTGTG ACATCATCAA ACATCCCATG 
GACATGAGCA CAATCAAGTC TAAACTAGAG TCCCGAGAGT ACAGAGATGC CCAGGAATTT 
GGTGCTGATG TCCGATTGAT GTTCTCCAAC TGCTACAAGT ACAACCCCCC TGACCATGAA 
GTGGTAGCCA TGGCTCGAAA ACTCCAGGAT GTGTTTGAAA TGCGCTTTGC CAAGATGCCT 
GATGAGCCTG AAGAGCCAGT TGTTACAGTG TCCTCTCCTG CAGTGCCACC CCCTACAAAG 
GTGGTAGCCC CACCCTCATC TAGTGACAGC AGCAGCGACA GTTCTTCCGA CAGCGACAGT 
TCCACTGACG ACTCTGAGGA AGAGCGAGCC CAGCGGCTGG CTGAACTCCA GGAACAGCTC 
AAGGCCGTGC ATGAGCAGCT TGCAGCCCTC TCACAGCCCC AGCAGAACAA ACCAAAGAAA 
AAGGAGAAGG ACAAGAAGGA AAAGAAAAAG GAAAAGCACA AAAAGAAAGA AGAAGTGGAG 
GAAAATAAAA AAAGCAAAAC CAAGGAACTT CCTCCCAAAA AGACAAAGAA AAATAACAGC 
AGCAACAGCA ATGTGAGCAA GAAGGAACCA GTACCCACGA AGACCAAGCC GCCTCCCACA 
TATGAATCAG AAGAGGAGGA TAAGTGTAAG CCCATGTCTT ATGAGGAGAA GCGGCAGCTA 
AGTCTAGATA TCAACAAACT TCCTGGTGAG AAGCTAGGCC GTGTAGTACA CATAATTCAG 
TCAAGGGAAC CATCACTTAA AAACTCCAAC CCCGATGAGA TTGAGATTGA CTTTGAGACC 
CTGAAGCCAT CTACACTACG AGAGTTGGAG CGATATGTCA CCTCCTGTTT GCGGAAGAAA 
AGGAAACCTC AAGCTGAAAA AGTTGACGTG ATTGCTGGTT CTTCCAAGAT GAAGGGATTC 
TCATCCTCTG AGTCGGAGAG CACCAGCGAA TCCAGCTCCT CTGACAGTGA AGACTCTGAA 
ACAGAGATGG CTCCCAAGTC AAAAAAGAAG GGGCACACTG GGAGGGACCA GAAAAAGCAT 
CATCACCATC ACCATCCACA GATGCAGCCA GCCCCAGCTC CTGTGCCCCA GCAGCCGCCC 
CCACCTCCAC AGCAGCCTCC TCCACCCCCA CCTCCGCAGC AGCAGCAGCA GCAACCTCCA 
CCCCCACCAC CTCCGCCCTC CATGCCACAG CAGACTGCCC CAGCGATGAA GTCCTCGCCC 
CCACCCTTCA TCACTGCCCA GGTCCCCGTC CTGGAACCAC AGCTGCCAGG CAGTGTCTTT 
GACCCTATTG GCCACTTCAC CCAGCCCATC TTGCACCTGC CGCAGCCGGA GCTGCCTCCT 
CACCTGCCCC AGCCACCTGA GCACAGCACT CCACCCCATC TCAACCAGCA TGCTGTGGTC 
TCTCCTCCAG CTTTGCACAA TGCGCTGCCC CAACAGCCAT CTCGGCCCAG TAACCGAGCT 
GCTGCTCTGC CCCCAAAGCC TACCCGACCC CCAGCTGTGT CCCCTGCCCT GGCCCAGCCC 
CCCCTGCTCC CACAACCACC AATGGCTCAG CCCCCCCAAG TGCTGCTGGA GGATGAAGAG 
CCACCTGCCC CACCCCTCAC CTCCATGCAG ATGCAGTTGT ACCTACAGCA GCTGCAGAAG 
GTGCAGCCTC CCACACCACT ACTCCCTTCC GTGAAGGTGC AGTCCCAACC CCCACCGCCT 
TTGCCGCCTC CACCCCACCC TTCTGTGCAG CAGCAGCAGC TCCAGCCACA GCCACCGCCA 
CCCCCACCTC CTCAGCCACA GCCACCACCC CAGCAACAAC ACCAGCCTCC ACCACGACCA 
GTTCACTTGC CATCCATGCC CTTTTCTGCT CATATTCAGC AGCCCCCACC ACCCCCAGGA 
CAGCAACCTA CTCACCCACC CCCAGGACAG CAGCCCCCAC CACCACAGCC TGCCAAGCCC 
CAGCAAGTCA TCCAGCATCA CCCTTCCCCC CGGCACCACA AGTCAGACCC CTACTCAGCT 
GGTCATCTTC GTGAGGCTCC CTCTCCGCTT ATGATACATT CCCCTCAGAT GCCACAGTTC 
CAGAGCCTGA CCCATCAGTC TCCTCCCCAG CAAAACGTCC AGCCTAAGAA GCAGGTAAAG 
GGCAGGGCTG AGCCACAGCC ACCAGGGCCA GTCATGGGCC AAGGCCAGGG ATGCCCACCT 
GCCTCACCGG CTGCCGTGCC TATGCTGTCC CAGGAGCTGC GACCACCTTC AGTCGTCCAG 
CCCCAGCCCC TGGTGGTAGT AAAGGAGGAG AAGATTCACT CACCAATCAT TCGCAGCGAG 
CCTTTCAGCA CCTCACTTCG ACCAGAGCCC CCCAAGCACC CAGAGAACAT CAAGGCCCCA 
GTCCACCTGC CCCAGCGGCC TGAGATGAAG CCTGTAGACA TAGGGAGGCC TGTGATCCGG 
CCTCCAGAGC AGAGTGCACC CCCACCAGGG GCCCCTGACA AGGACAAACA GAAGCAGGAG 
CCAAAAACAC CAGTGGCACC CAAAAAGGAC CTGAAAATTA AGAACATGGG CTCCTGGGCC 
AGCCTGGTAC AGAAGCATCC GACCACCCCA TCCTCCACAG CCAAGTCCTC AAGTGACAGC 
TTTGAGCATT TCCGCCGTGC TGCTCGGGAG AAGGAGGAGA GGGAAAAGGC CCTGAAGGCT 
CAGGCTGAGC ATGCAGAGAA GGAGAAGGAG CGGCTGAGGC AGGAGCGCAT GAGGAGCCGA 
GAGGATGAAG ATGCGCTGGA ACAGGCACGT CGAGCCCATG AGGAGGCACG ACGGCGCCAG 
GAGCAGCAGC AGCAGCAGCA GCAGCAGAGA CAGGAGCAGC AGCAGCAGCA GCAGCAGGCA 
GCTGCTGTGG CCGCTGCCTC TGCCCCCCAG GCCCAGAGCT CCCAGCCCCA GTCTATGCTG 
GACCAGCAGA GGGAGTTGGC CCGGAAACGA GAGCAGGAGC GGAGGCGCAG GGAGGCAATG 
GCAGCTACAA TTGACATGAA TTTCCAGAGT GATCTTTTGT CAATATTTGA AGAAAATCTT 
TTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105782.1
CDS	407..4612
Translation	MSTESGPGTRLRNLPVMGDGLETSQMSTTQAQAQPQPANAASTNPPPPETSNPNKPKRQTNQLQYLLRVVLKTLWKHQFAWPFQQPVDAVKLNLPDYYKIIKTPMDMGTIKKRLENNYYWNAQECIQDFNTMFTNCYIYNKPGDDIVLMAEALEKLFLQKINELPTEETEIMIVQAKGRGRGRKETGRAAKPGVSTVPNTTQASTSPQTQTPQQNPPPPVQATTHPFPAVTPDLIAQPPVMTMVPPQPLQTPSPVPPQPPPPPAPVPQPVQSHPPIIATTPQPVKTKKGVKRKADTTTPTTIDPIHEPPSLAPEPKTAKLGPRRESSRPVKPPKKDVPDSQQHPGPEKSSKISEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYCDIIKHPMDMSTIKSKLESREYRDAQEFGADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPEEPVVTVSSPAVPPPTKVVAPPSSSDSSSDSSSDSDSSTDDSEEERAQRLAELQEQLKAVHEQLAALSQPQQNKPKKKEKDKKEKKKEKHKKKEEVEENKKSKTKELPPKKTKKNNSSNSNVSKKEPVPTKTKPPPTYESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVDVIAGSSKMKGFSSSESESTSESSSSDSEDSETEMAPKSKKKGHTGRDQKKHHHHHHPQMQPAPAPVPQQPPPPPQQPPPPPPPQQQQQQPPPPPPPPSMPQQTAPAMKSSPPPFITAQVPVLEPQLPGSVFDPIGHFTQPILHLPQPELPPHLPQPPEHSTPPHLNQHAVVSPPALHNALPQQPSRPSNRAAALPPKPTRPPAVSPALAQPPLLPQPPMAQPPQVLLEDEEPPAPPLTSMQMQLYLQQLQKVQPPTPLLPSVKVQSQPPPPLPPPPHPSVQQQQLQPQPPPPPPPQPQPPPQQQHQPPPRPVHLPSMPFSAHIQQPPPPPGQQPTHPPPGQQPPPPQPAKPQQVIQHHPSPRHHKSDPYSAGHLREAPSPLMIHSPQMPQFQSLTHQSPPQQNVQPKKQVKGRAEPQPPGPVMGQGQGCPPASPAAVPMLSQELRPPSVVQPQPLVVVKEEKIHSPIIRSEPFSTSLRPEPPKHPENIKAPVHLPQRPEMKPVDIGRPVIRPPEQSAPPPGAPDKDKQKQEPKTPVAPKKDLKIKNMGSWASLVQKHPTTPSSTAKSSSDSFEHFRRAAREKEEREKALKAQAEHAEKEKERLRQERMRSREDEDALEQARRAHEEARRRQEQQQQQQQQRQEQQQQQQQAAAVAAASAPQAQSSQPQSMLDQQRELARKREQERRRREAMAATIDMNFQSDLLSIFEENLF

Target ORF information:

RefSeq Version	XM_030249922.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus bromodomain containing 4 (Brd4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249922.1

ORF Insert Sequence:

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGCC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGACAGACA 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCCGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAAAAA 
ATCAATGAAC TGCCTACAGA AGAAACTGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGAGGGAGGA AAGAAACAGG TAGGGCAGCA AAGCCTGGTG TATCCACGGT ACCAAACACA 
ACTCAAGCAT CAACTTCTCC GCAGACCCAG ACGCCTCAGC AGAACCCTCC TCCACCTGTG 
CAGGCCACAA CTCACCCCTT TCCTGCTGTC ACCCCAGACC TCATTGCCCA GCCTCCTGTC 
ATGACAATGG TGCCCCCTCA GCCACTTCAG ACTCCTTCAC CGGTACCCCC CCAGCCACCA 
CCCCCACCTG CTCCAGTTCC ACAGCCTGTG CAGAGTCACC CGCCCATCAT TGCGACCACC 
CCCCAGCCTG TGAAGACAAA GAAAGGGGTG AAGAGGAAAG CAGATACCAC CACCCCTACC 
ACCATCGACC CCATTCATGA GCCACCCTCA CTGGCCCCAG AGCCCAAGAC CGCCAAGCTG 
GGTCCTCGGC GGGAGAGCAG CAGACCTGTG AAGCCTCCAA AGAAGGATGT ACCGGACTCA 
CAGCAGCACC CAGGGCCAGA GAAGAGCAGC AAGATCTCTG AGCAGCTAAA GTGCTGCAGT 
GGCATCCTCA AGGAGATGTT TGCCAAGAAA CATGCTGCCT ATGCCTGGCC TTTCTACAAG 
CCTGTGGATG TGGAGGCACT GGGTCTGCAC GACTACTGTG ACATCATCAA ACATCCCATG 
GACATGAGCA CAATCAAGTC TAAACTAGAG TCCCGAGAGT ACAGAGATGC CCAGGAATTT 
GGTGCTGATG TCCGATTGAT GTTCTCCAAC TGCTACAAGT ACAACCCCCC TGACCATGAA 
GTGGTAGCCA TGGCTCGAAA ACTCCAGGAT GTGTTTGAAA TGCGCTTTGC CAAGATGCCT 
GATGAGCCTG AAGAGCCAGT TGTTACAGTG TCCTCTCCTG CAGTGCCACC CCCTACAAAG 
GTGGTAGCCC CACCCTCATC TAGTGACAGC AGCAGCGACA GTTCTTCCGA CAGCGACAGT 
TCCACTGACG ACTCTGAGGA AGAGCGAGCC CAGCGGCTGG CTGAACTCCA GGAACAGCTC 
AAGGCCGTGC ATGAGCAGCT TGCAGCCCTC TCACAGCCCC AGCAGAACAA ACCAAAGAAA 
AAGGAGAAGG ACAAGAAGGA AAAGAAAAAG GAAAAGCACA AAAAGAAAGA AGAAGTGGAG 
GAAAATAAAA AAAGCAAAAC CAAGGAACTT CCTCCCAAAA AGACAAAGAA AAATAACAGC 
AGCAACAGCA ATGTGAGCAA GAAGGAACCA GTACCCACGA AGACCAAGCC GCCTCCCACA 
TATGAATCAG AAGAGGAGGA TAAGTGTAAG CCCATGTCTT ATGAGGAGAA GCGGCAGCTA 
AGTCTAGATA TCAACAAACT TCCTGGTGAG AAGCTAGGCC GTGTAGTACA CATAATTCAG 
TCAAGGGAAC CATCACTTAA AAACTCCAAC CCCGATGAGA TTGAGATTGA CTTTGAGACC 
CTGAAGCCAT CTACACTACG AGAGTTGGAG CGATATGTCA CCTCCTGTTT GCGGAAGAAA 
AGGAAACCTC AAGCTGAAAA AGTTGACGTG ATTGCTGGTT CTTCCAAGAT GAAGGGATTC 
TCATCCTCTG AGTCGGAGAG CACCAGCGAA TCCAGCTCCT CTGACAGTGA AGACTCTGAA 
ACAGAGATGG CTCCCAAGTC AAAAAAGAAG GGGCACACTG GGAGGGACCA GAAAAAGCAT 
CATCACCATC ACCATCCACA GATGCAGCCA GCCCCAGCTC CTGTGCCCCA GCAGCCGCCC 
CCACCTCCAC AGCAGCCTCC TCCACCCCCA CCTCCGCAGC AGCAGCAGCA GCAACCTCCA 
CCCCCACCAC CTCCGCCCTC CATGCCACAG CAGACTGCCC CAGCGATGAA GTCCTCGCCC 
CCACCCTTCA TCACTGCCCA GGTCCCCGTC CTGGAACCAC AGCTGCCAGG CAGTGTCTTT 
GACCCTATTG GCCACTTCAC CCAGCCCATC TTGCACCTGC CGCAGCCGGA GCTGCCTCCT 
CACCTGCCCC AGCCACCTGA GCACAGCACT CCACCCCATC TCAACCAGCA TGCTGTGGTC 
TCTCCTCCAG CTTTGCACAA TGCGCTGCCC CAACAGCCAT CTCGGCCCAG TAACCGAGCT 
GCTGCTCTGC CCCCAAAGCC TACCCGACCC CCAGCTGTGT CCCCTGCCCT GGCCCAGCCC 
CCCCTGCTCC CACAACCACC AATGGCTCAG CCCCCCCAAG TGCTGCTGGA GGATGAAGAG 
CCACCTGCCC CACCCCTCAC CTCCATGCAG ATGCAGTTGT ACCTACAGCA GCTGCAGAAG 
GTGCAGCCTC CCACACCACT ACTCCCTTCC GTGAAGGTGC AGTCCCAACC CCCACCGCCT 
TTGCCGCCTC CACCCCACCC TTCTGTGCAG CAGCAGCAGC TCCAGCCACA GCCACCGCCA 
CCCCCACCTC CTCAGCCACA GCCACCACCC CAGCAACAAC ACCAGCCTCC ACCACGACCA 
GTTCACTTGC CATCCATGCC CTTTTCTGCT CATATTCAGC AGCCCCCACC ACCCCCAGGA 
CAGCAACCTA CTCACCCACC CCCAGGACAG CAGCCCCCAC CACCACAGCC TGCCAAGCCC 
CAGCAAGTCA TCCAGCATCA CCCTTCCCCC CGGCACCACA AGTCAGACCC CTACTCAGCT 
GGTCATCTTC GTGAGGCTCC CTCTCCGCTT ATGATACATT CCCCTCAGAT GCCACAGTTC 
CAGAGCCTGA CCCATCAGTC TCCTCCCCAG CAAAACGTCC AGCCTAAGAA GCAGGTAAAG 
GGCAGGGCTG AGCCACAGCC ACCAGGGCCA GTCATGGGCC AAGGCCAGGG ATGCCCACCT 
GCCTCACCGG CTGCCGTGCC TATGCTGTCC CAGGAGCTGC GACCACCTTC AGTCGTCCAG 
CCCCAGCCCC TGGTGGTAGT AAAGGAGGAG AAGATTCACT CACCAATCAT TCGCAGCGAG 
CCTTTCAGCA CCTCACTTCG ACCAGAGCCC CCCAAGCACC CAGAGAACAT CAAGGCCCCA 
GTCCACCTGC CCCAGCGGCC TGAGATGAAG CCTGTAGACA TAGGGAGGCC TGTGATCCGG 
CCTCCAGAGC AGAGTGCACC CCCACCAGGG GCCCCTGACA AGGACAAACA GAAGCAGGAG 
CCAAAAACAC CAGTGGCACC CAAAAAGGAC CTGAAAATTA AGAACATGGG CTCCTGGGCC 
AGCCTGGTAC AGAAGCATCC GACCACCCCA TCCTCCACAG CCAAGTCCTC AAGTGACAGC 
TTTGAGCATT TCCGCCGTGC TGCTCGGGAG AAGGAGGAGA GGGAAAAGGC CCTGAAGGCT 
CAGGCTGAGC ATGCAGAGAA GGAGAAGGAG CGGCTGAGGC AGGAGCGCAT GAGGAGCCGA 
GAGGATGAAG ATGCGCTGGA ACAGGCACGT CGAGCCCATG AGGAGGCACG ACGGCGCCAG 
GAGCAGCAGC AGCAGCAGCA GCAGCAGAGA CAGGAGCAGC AGCAGCAGCA GCAGCAGGCA 
GCTGCTGTGG CCGCTGCCTC TGCCCCCCAG GCCCAGAGCT CCCAGCCCCA GTCTATGCTG 
GACCAGCAGA GGGAGTTGGC CCGGAAACGA GAGCAGGAGC GGAGGCGCAG GGAGGCAATG 
GCAGCTACAA TTGACATGAA TTTCCAGAGT GATCTTTTGT CAATATTTGA AGAAAATCTT 
TTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brd4 ( XM_006524686.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524749.1 Mus musculus bromodomain containing 4 (Brd4), transcript variant X6, mRNA.

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CloneID	OMu17143
Clone ID Related Accession (Same CDS sequence)	XM_006524683.2 , XM_006524683.3 , XM_006524684.2 , XM_030249922.1 , XM_006524686.2 , XM_006524687.2 , XM_006524685.2 , XM_030249923.1 , NM_001286630.1 , XM_006524685.1 , XM_006524686.1 , XM_006524684.3
Accession Version	XM_006524686.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4206bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	bromodomain-containing protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006524686.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGCC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGACAGACA 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCCGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAAAAA 
ATCAATGAAC TGCCTACAGA AGAAACTGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGAGGGAGGA AAGAAACAGG TAGGGCAGCA AAGCCTGGTG TATCCACGGT ACCAAACACA 
ACTCAAGCAT CAACTTCTCC GCAGACCCAG ACGCCTCAGC AGAACCCTCC TCCACCTGTG 
CAGGCCACAA CTCACCCCTT TCCTGCTGTC ACCCCAGACC TCATTGCCCA GCCTCCTGTC 
ATGACAATGG TGCCCCCTCA GCCACTTCAG ACTCCTTCAC CGGTACCCCC CCAGCCACCA 
CCCCCACCTG CTCCAGTTCC ACAGCCTGTG CAGAGTCACC CGCCCATCAT TGCGACCACC 
CCCCAGCCTG TGAAGACAAA GAAAGGGGTG AAGAGGAAAG CAGATACCAC CACCCCTACC 
ACCATCGACC CCATTCATGA GCCACCCTCA CTGGCCCCAG AGCCCAAGAC CGCCAAGCTG 
GGTCCTCGGC GGGAGAGCAG CAGACCTGTG AAGCCTCCAA AGAAGGATGT ACCGGACTCA 
CAGCAGCACC CAGGGCCAGA GAAGAGCAGC AAGATCTCTG AGCAGCTAAA GTGCTGCAGT 
GGCATCCTCA AGGAGATGTT TGCCAAGAAA CATGCTGCCT ATGCCTGGCC TTTCTACAAG 
CCTGTGGATG TGGAGGCACT GGGTCTGCAC GACTACTGTG ACATCATCAA ACATCCCATG 
GACATGAGCA CAATCAAGTC TAAACTAGAG TCCCGAGAGT ACAGAGATGC CCAGGAATTT 
GGTGCTGATG TCCGATTGAT GTTCTCCAAC TGCTACAAGT ACAACCCCCC TGACCATGAA 
GTGGTAGCCA TGGCTCGAAA ACTCCAGGAT GTGTTTGAAA TGCGCTTTGC CAAGATGCCT 
GATGAGCCTG AAGAGCCAGT TGTTACAGTG TCCTCTCCTG CAGTGCCACC CCCTACAAAG 
GTGGTAGCCC CACCCTCATC TAGTGACAGC AGCAGCGACA GTTCTTCCGA CAGCGACAGT 
TCCACTGACG ACTCTGAGGA AGAGCGAGCC CAGCGGCTGG CTGAACTCCA GGAACAGCTC 
AAGGCCGTGC ATGAGCAGCT TGCAGCCCTC TCACAGCCCC AGCAGAACAA ACCAAAGAAA 
AAGGAGAAGG ACAAGAAGGA AAAGAAAAAG GAAAAGCACA AAAAGAAAGA AGAAGTGGAG 
GAAAATAAAA AAAGCAAAAC CAAGGAACTT CCTCCCAAAA AGACAAAGAA AAATAACAGC 
AGCAACAGCA ATGTGAGCAA GAAGGAACCA GTACCCACGA AGACCAAGCC GCCTCCCACA 
TATGAATCAG AAGAGGAGGA TAAGTGTAAG CCCATGTCTT ATGAGGAGAA GCGGCAGCTA 
AGTCTAGATA TCAACAAACT TCCTGGTGAG AAGCTAGGCC GTGTAGTACA CATAATTCAG 
TCAAGGGAAC CATCACTTAA AAACTCCAAC CCCGATGAGA TTGAGATTGA CTTTGAGACC 
CTGAAGCCAT CTACACTACG AGAGTTGGAG CGATATGTCA CCTCCTGTTT GCGGAAGAAA 
AGGAAACCTC AAGCTGAAAA AGTTGACGTG ATTGCTGGTT CTTCCAAGAT GAAGGGATTC 
TCATCCTCTG AGTCGGAGAG CACCAGCGAA TCCAGCTCCT CTGACAGTGA AGACTCTGAA 
ACAGAGATGG CTCCCAAGTC AAAAAAGAAG GGGCACACTG GGAGGGACCA GAAAAAGCAT 
CATCACCATC ACCATCCACA GATGCAGCCA GCCCCAGCTC CTGTGCCCCA GCAGCCGCCC 
CCACCTCCAC AGCAGCCTCC TCCACCCCCA CCTCCGCAGC AGCAGCAGCA GCAACCTCCA 
CCCCCACCAC CTCCGCCCTC CATGCCACAG CAGACTGCCC CAGCGATGAA GTCCTCGCCC 
CCACCCTTCA TCACTGCCCA GGTCCCCGTC CTGGAACCAC AGCTGCCAGG CAGTGTCTTT 
GACCCTATTG GCCACTTCAC CCAGCCCATC TTGCACCTGC CGCAGCCGGA GCTGCCTCCT 
CACCTGCCCC AGCCACCTGA GCACAGCACT CCACCCCATC TCAACCAGCA TGCTGTGGTC 
TCTCCTCCAG CTTTGCACAA TGCGCTGCCC CAACAGCCAT CTCGGCCCAG TAACCGAGCT 
GCTGCTCTGC CCCCAAAGCC TACCCGACCC CCAGCTGTGT CCCCTGCCCT GGCCCAGCCC 
CCCCTGCTCC CACAACCACC AATGGCTCAG CCCCCCCAAG TGCTGCTGGA GGATGAAGAG 
CCACCTGCCC CACCCCTCAC CTCCATGCAG ATGCAGTTGT ACCTACAGCA GCTGCAGAAG 
GTGCAGCCTC CCACACCACT ACTCCCTTCC GTGAAGGTGC AGTCCCAACC CCCACCGCCT 
TTGCCGCCTC CACCCCACCC TTCTGTGCAG CAGCAGCAGC TCCAGCCACA GCCACCGCCA 
CCCCCACCTC CTCAGCCACA GCCACCACCC CAGCAACAAC ACCAGCCTCC ACCACGACCA 
GTTCACTTGC CATCCATGCC CTTTTCTGCT CATATTCAGC AGCCCCCACC ACCCCCAGGA 
CAGCAACCTA CTCACCCACC CCCAGGACAG CAGCCCCCAC CACCACAGCC TGCCAAGCCC 
CAGCAAGTCA TCCAGCATCA CCCTTCCCCC CGGCACCACA AGTCAGACCC CTACTCAGCT 
GGTCATCTTC GTGAGGCTCC CTCTCCGCTT ATGATACATT CCCCTCAGAT GCCACAGTTC 
CAGAGCCTGA CCCATCAGTC TCCTCCCCAG CAAAACGTCC AGCCTAAGAA GCAGGTAAAG 
GGCAGGGCTG AGCCACAGCC ACCAGGGCCA GTCATGGGCC AAGGCCAGGG ATGCCCACCT 
GCCTCACCGG CTGCCGTGCC TATGCTGTCC CAGGAGCTGC GACCACCTTC AGTCGTCCAG 
CCCCAGCCCC TGGTGGTAGT AAAGGAGGAG AAGATTCACT CACCAATCAT TCGCAGCGAG 
CCTTTCAGCA CCTCACTTCG ACCAGAGCCC CCCAAGCACC CAGAGAACAT CAAGGCCCCA 
GTCCACCTGC CCCAGCGGCC TGAGATGAAG CCTGTAGACA TAGGGAGGCC TGTGATCCGG 
CCTCCAGAGC AGAGTGCACC CCCACCAGGG GCCCCTGACA AGGACAAACA GAAGCAGGAG 
CCAAAAACAC CAGTGGCACC CAAAAAGGAC CTGAAAATTA AGAACATGGG CTCCTGGGCC 
AGCCTGGTAC AGAAGCATCC GACCACCCCA TCCTCCACAG CCAAGTCCTC AAGTGACAGC 
TTTGAGCATT TCCGCCGTGC TGCTCGGGAG AAGGAGGAGA GGGAAAAGGC CCTGAAGGCT 
CAGGCTGAGC ATGCAGAGAA GGAGAAGGAG CGGCTGAGGC AGGAGCGCAT GAGGAGCCGA 
GAGGATGAAG ATGCGCTGGA ACAGGCACGT CGAGCCCATG AGGAGGCACG ACGGCGCCAG 
GAGCAGCAGC AGCAGCAGCA GCAGCAGAGA CAGGAGCAGC AGCAGCAGCA GCAGCAGGCA 
GCTGCTGTGG CCGCTGCCTC TGCCCCCCAG GCCCAGAGCT CCCAGCCCCA GTCTATGCTG 
GACCAGCAGA GGGAGTTGGC CCGGAAACGA GAGCAGGAGC GGAGGCGCAG GGAGGCAATG 
GCAGCTACAA TTGACATGAA TTTCCAGAGT GATCTTTTGT CAATATTTGA AGAAAATCTT 
TTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524749.1
CDS	136..4341
Translation	MSTESGPGTRLRNLPVMGDGLETSQMSTTQAQAQPQPANAASTNPPPPETSNPNKPKRQTNQLQYLLRVVLKTLWKHQFAWPFQQPVDAVKLNLPDYYKIIKTPMDMGTIKKRLENNYYWNAQECIQDFNTMFTNCYIYNKPGDDIVLMAEALEKLFLQKINELPTEETEIMIVQAKGRGRGRKETGRAAKPGVSTVPNTTQASTSPQTQTPQQNPPPPVQATTHPFPAVTPDLIAQPPVMTMVPPQPLQTPSPVPPQPPPPPAPVPQPVQSHPPIIATTPQPVKTKKGVKRKADTTTPTTIDPIHEPPSLAPEPKTAKLGPRRESSRPVKPPKKDVPDSQQHPGPEKSSKISEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYCDIIKHPMDMSTIKSKLESREYRDAQEFGADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPEEPVVTVSSPAVPPPTKVVAPPSSSDSSSDSSSDSDSSTDDSEEERAQRLAELQEQLKAVHEQLAALSQPQQNKPKKKEKDKKEKKKEKHKKKEEVEENKKSKTKELPPKKTKKNNSSNSNVSKKEPVPTKTKPPPTYESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVDVIAGSSKMKGFSSSESESTSESSSSDSEDSETEMAPKSKKKGHTGRDQKKHHHHHHPQMQPAPAPVPQQPPPPPQQPPPPPPPQQQQQQPPPPPPPPSMPQQTAPAMKSSPPPFITAQVPVLEPQLPGSVFDPIGHFTQPILHLPQPELPPHLPQPPEHSTPPHLNQHAVVSPPALHNALPQQPSRPSNRAAALPPKPTRPPAVSPALAQPPLLPQPPMAQPPQVLLEDEEPPAPPLTSMQMQLYLQQLQKVQPPTPLLPSVKVQSQPPPPLPPPPHPSVQQQQLQPQPPPPPPPQPQPPPQQQHQPPPRPVHLPSMPFSAHIQQPPPPPGQQPTHPPPGQQPPPPQPAKPQQVIQHHPSPRHHKSDPYSAGHLREAPSPLMIHSPQMPQFQSLTHQSPPQQNVQPKKQVKGRAEPQPPGPVMGQGQGCPPASPAAVPMLSQELRPPSVVQPQPLVVVKEEKIHSPIIRSEPFSTSLRPEPPKHPENIKAPVHLPQRPEMKPVDIGRPVIRPPEQSAPPPGAPDKDKQKQEPKTPVAPKKDLKIKNMGSWASLVQKHPTTPSSTAKSSSDSFEHFRRAAREKEEREKALKAQAEHAEKEKERLRQERMRSREDEDALEQARRAHEEARRRQEQQQQQQQQRQEQQQQQQQAAAVAAASAPQAQSSQPQSMLDQQRELARKREQERRRREAMAATIDMNFQSDLLSIFEENLF

Target ORF information:

RefSeq Version	XM_006524686.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus bromodomain containing 4 (Brd4), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524686.2

ORF Insert Sequence:

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGCC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGACAGACA 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCCGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAAAAA 
ATCAATGAAC TGCCTACAGA AGAAACTGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGAGGGAGGA AAGAAACAGG TAGGGCAGCA AAGCCTGGTG TATCCACGGT ACCAAACACA 
ACTCAAGCAT CAACTTCTCC GCAGACCCAG ACGCCTCAGC AGAACCCTCC TCCACCTGTG 
CAGGCCACAA CTCACCCCTT TCCTGCTGTC ACCCCAGACC TCATTGCCCA GCCTCCTGTC 
ATGACAATGG TGCCCCCTCA GCCACTTCAG ACTCCTTCAC CGGTACCCCC CCAGCCACCA 
CCCCCACCTG CTCCAGTTCC ACAGCCTGTG CAGAGTCACC CGCCCATCAT TGCGACCACC 
CCCCAGCCTG TGAAGACAAA GAAAGGGGTG AAGAGGAAAG CAGATACCAC CACCCCTACC 
ACCATCGACC CCATTCATGA GCCACCCTCA CTGGCCCCAG AGCCCAAGAC CGCCAAGCTG 
GGTCCTCGGC GGGAGAGCAG CAGACCTGTG AAGCCTCCAA AGAAGGATGT ACCGGACTCA 
CAGCAGCACC CAGGGCCAGA GAAGAGCAGC AAGATCTCTG AGCAGCTAAA GTGCTGCAGT 
GGCATCCTCA AGGAGATGTT TGCCAAGAAA CATGCTGCCT ATGCCTGGCC TTTCTACAAG 
CCTGTGGATG TGGAGGCACT GGGTCTGCAC GACTACTGTG ACATCATCAA ACATCCCATG 
GACATGAGCA CAATCAAGTC TAAACTAGAG TCCCGAGAGT ACAGAGATGC CCAGGAATTT 
GGTGCTGATG TCCGATTGAT GTTCTCCAAC TGCTACAAGT ACAACCCCCC TGACCATGAA 
GTGGTAGCCA TGGCTCGAAA ACTCCAGGAT GTGTTTGAAA TGCGCTTTGC CAAGATGCCT 
GATGAGCCTG AAGAGCCAGT TGTTACAGTG TCCTCTCCTG CAGTGCCACC CCCTACAAAG 
GTGGTAGCCC CACCCTCATC TAGTGACAGC AGCAGCGACA GTTCTTCCGA CAGCGACAGT 
TCCACTGACG ACTCTGAGGA AGAGCGAGCC CAGCGGCTGG CTGAACTCCA GGAACAGCTC 
AAGGCCGTGC ATGAGCAGCT TGCAGCCCTC TCACAGCCCC AGCAGAACAA ACCAAAGAAA 
AAGGAGAAGG ACAAGAAGGA AAAGAAAAAG GAAAAGCACA AAAAGAAAGA AGAAGTGGAG 
GAAAATAAAA AAAGCAAAAC CAAGGAACTT CCTCCCAAAA AGACAAAGAA AAATAACAGC 
AGCAACAGCA ATGTGAGCAA GAAGGAACCA GTACCCACGA AGACCAAGCC GCCTCCCACA 
TATGAATCAG AAGAGGAGGA TAAGTGTAAG CCCATGTCTT ATGAGGAGAA GCGGCAGCTA 
AGTCTAGATA TCAACAAACT TCCTGGTGAG AAGCTAGGCC GTGTAGTACA CATAATTCAG 
TCAAGGGAAC CATCACTTAA AAACTCCAAC CCCGATGAGA TTGAGATTGA CTTTGAGACC 
CTGAAGCCAT CTACACTACG AGAGTTGGAG CGATATGTCA CCTCCTGTTT GCGGAAGAAA 
AGGAAACCTC AAGCTGAAAA AGTTGACGTG ATTGCTGGTT CTTCCAAGAT GAAGGGATTC 
TCATCCTCTG AGTCGGAGAG CACCAGCGAA TCCAGCTCCT CTGACAGTGA AGACTCTGAA 
ACAGAGATGG CTCCCAAGTC AAAAAAGAAG GGGCACACTG GGAGGGACCA GAAAAAGCAT 
CATCACCATC ACCATCCACA GATGCAGCCA GCCCCAGCTC CTGTGCCCCA GCAGCCGCCC 
CCACCTCCAC AGCAGCCTCC TCCACCCCCA CCTCCGCAGC AGCAGCAGCA GCAACCTCCA 
CCCCCACCAC CTCCGCCCTC CATGCCACAG CAGACTGCCC CAGCGATGAA GTCCTCGCCC 
CCACCCTTCA TCACTGCCCA GGTCCCCGTC CTGGAACCAC AGCTGCCAGG CAGTGTCTTT 
GACCCTATTG GCCACTTCAC CCAGCCCATC TTGCACCTGC CGCAGCCGGA GCTGCCTCCT 
CACCTGCCCC AGCCACCTGA GCACAGCACT CCACCCCATC TCAACCAGCA TGCTGTGGTC 
TCTCCTCCAG CTTTGCACAA TGCGCTGCCC CAACAGCCAT CTCGGCCCAG TAACCGAGCT 
GCTGCTCTGC CCCCAAAGCC TACCCGACCC CCAGCTGTGT CCCCTGCCCT GGCCCAGCCC 
CCCCTGCTCC CACAACCACC AATGGCTCAG CCCCCCCAAG TGCTGCTGGA GGATGAAGAG 
CCACCTGCCC CACCCCTCAC CTCCATGCAG ATGCAGTTGT ACCTACAGCA GCTGCAGAAG 
GTGCAGCCTC CCACACCACT ACTCCCTTCC GTGAAGGTGC AGTCCCAACC CCCACCGCCT 
TTGCCGCCTC CACCCCACCC TTCTGTGCAG CAGCAGCAGC TCCAGCCACA GCCACCGCCA 
CCCCCACCTC CTCAGCCACA GCCACCACCC CAGCAACAAC ACCAGCCTCC ACCACGACCA 
GTTCACTTGC CATCCATGCC CTTTTCTGCT CATATTCAGC AGCCCCCACC ACCCCCAGGA 
CAGCAACCTA CTCACCCACC CCCAGGACAG CAGCCCCCAC CACCACAGCC TGCCAAGCCC 
CAGCAAGTCA TCCAGCATCA CCCTTCCCCC CGGCACCACA AGTCAGACCC CTACTCAGCT 
GGTCATCTTC GTGAGGCTCC CTCTCCGCTT ATGATACATT CCCCTCAGAT GCCACAGTTC 
CAGAGCCTGA CCCATCAGTC TCCTCCCCAG CAAAACGTCC AGCCTAAGAA GCAGGTAAAG 
GGCAGGGCTG AGCCACAGCC ACCAGGGCCA GTCATGGGCC AAGGCCAGGG ATGCCCACCT 
GCCTCACCGG CTGCCGTGCC TATGCTGTCC CAGGAGCTGC GACCACCTTC AGTCGTCCAG 
CCCCAGCCCC TGGTGGTAGT AAAGGAGGAG AAGATTCACT CACCAATCAT TCGCAGCGAG 
CCTTTCAGCA CCTCACTTCG ACCAGAGCCC CCCAAGCACC CAGAGAACAT CAAGGCCCCA 
GTCCACCTGC CCCAGCGGCC TGAGATGAAG CCTGTAGACA TAGGGAGGCC TGTGATCCGG 
CCTCCAGAGC AGAGTGCACC CCCACCAGGG GCCCCTGACA AGGACAAACA GAAGCAGGAG 
CCAAAAACAC CAGTGGCACC CAAAAAGGAC CTGAAAATTA AGAACATGGG CTCCTGGGCC 
AGCCTGGTAC AGAAGCATCC GACCACCCCA TCCTCCACAG CCAAGTCCTC AAGTGACAGC 
TTTGAGCATT TCCGCCGTGC TGCTCGGGAG AAGGAGGAGA GGGAAAAGGC CCTGAAGGCT 
CAGGCTGAGC ATGCAGAGAA GGAGAAGGAG CGGCTGAGGC AGGAGCGCAT GAGGAGCCGA 
GAGGATGAAG ATGCGCTGGA ACAGGCACGT CGAGCCCATG AGGAGGCACG ACGGCGCCAG 
GAGCAGCAGC AGCAGCAGCA GCAGCAGAGA CAGGAGCAGC AGCAGCAGCA GCAGCAGGCA 
GCTGCTGTGG CCGCTGCCTC TGCCCCCCAG GCCCAGAGCT CCCAGCCCCA GTCTATGCTG 
GACCAGCAGA GGGAGTTGGC CCGGAAACGA GAGCAGGAGC GGAGGCGCAG GGAGGCAATG 
GCAGCTACAA TTGACATGAA TTTCCAGAGT GATCTTTTGT CAATATTTGA AGAAAATCTT 
TTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brd4 ( XM_006524687.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524750.1 Mus musculus bromodomain containing 4 (Brd4), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu17143
Clone ID Related Accession (Same CDS sequence)	XM_006524683.2 , XM_006524683.3 , XM_006524684.2 , XM_030249922.1 , XM_006524686.2 , XM_006524687.2 , XM_006524685.2 , XM_030249923.1 , NM_001286630.1 , XM_006524685.1 , XM_006524686.1 , XM_006524684.3
Accession Version	XM_006524687.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4206bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	bromodomain-containing protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006524687.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGCC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGACAGACA 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCCGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAAAAA 
ATCAATGAAC TGCCTACAGA AGAAACTGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGAGGGAGGA AAGAAACAGG TAGGGCAGCA AAGCCTGGTG TATCCACGGT ACCAAACACA 
ACTCAAGCAT CAACTTCTCC GCAGACCCAG ACGCCTCAGC AGAACCCTCC TCCACCTGTG 
CAGGCCACAA CTCACCCCTT TCCTGCTGTC ACCCCAGACC TCATTGCCCA GCCTCCTGTC 
ATGACAATGG TGCCCCCTCA GCCACTTCAG ACTCCTTCAC CGGTACCCCC CCAGCCACCA 
CCCCCACCTG CTCCAGTTCC ACAGCCTGTG CAGAGTCACC CGCCCATCAT TGCGACCACC 
CCCCAGCCTG TGAAGACAAA GAAAGGGGTG AAGAGGAAAG CAGATACCAC CACCCCTACC 
ACCATCGACC CCATTCATGA GCCACCCTCA CTGGCCCCAG AGCCCAAGAC CGCCAAGCTG 
GGTCCTCGGC GGGAGAGCAG CAGACCTGTG AAGCCTCCAA AGAAGGATGT ACCGGACTCA 
CAGCAGCACC CAGGGCCAGA GAAGAGCAGC AAGATCTCTG AGCAGCTAAA GTGCTGCAGT 
GGCATCCTCA AGGAGATGTT TGCCAAGAAA CATGCTGCCT ATGCCTGGCC TTTCTACAAG 
CCTGTGGATG TGGAGGCACT GGGTCTGCAC GACTACTGTG ACATCATCAA ACATCCCATG 
GACATGAGCA CAATCAAGTC TAAACTAGAG TCCCGAGAGT ACAGAGATGC CCAGGAATTT 
GGTGCTGATG TCCGATTGAT GTTCTCCAAC TGCTACAAGT ACAACCCCCC TGACCATGAA 
GTGGTAGCCA TGGCTCGAAA ACTCCAGGAT GTGTTTGAAA TGCGCTTTGC CAAGATGCCT 
GATGAGCCTG AAGAGCCAGT TGTTACAGTG TCCTCTCCTG CAGTGCCACC CCCTACAAAG 
GTGGTAGCCC CACCCTCATC TAGTGACAGC AGCAGCGACA GTTCTTCCGA CAGCGACAGT 
TCCACTGACG ACTCTGAGGA AGAGCGAGCC CAGCGGCTGG CTGAACTCCA GGAACAGCTC 
AAGGCCGTGC ATGAGCAGCT TGCAGCCCTC TCACAGCCCC AGCAGAACAA ACCAAAGAAA 
AAGGAGAAGG ACAAGAAGGA AAAGAAAAAG GAAAAGCACA AAAAGAAAGA AGAAGTGGAG 
GAAAATAAAA AAAGCAAAAC CAAGGAACTT CCTCCCAAAA AGACAAAGAA AAATAACAGC 
AGCAACAGCA ATGTGAGCAA GAAGGAACCA GTACCCACGA AGACCAAGCC GCCTCCCACA 
TATGAATCAG AAGAGGAGGA TAAGTGTAAG CCCATGTCTT ATGAGGAGAA GCGGCAGCTA 
AGTCTAGATA TCAACAAACT TCCTGGTGAG AAGCTAGGCC GTGTAGTACA CATAATTCAG 
TCAAGGGAAC CATCACTTAA AAACTCCAAC CCCGATGAGA TTGAGATTGA CTTTGAGACC 
CTGAAGCCAT CTACACTACG AGAGTTGGAG CGATATGTCA CCTCCTGTTT GCGGAAGAAA 
AGGAAACCTC AAGCTGAAAA AGTTGACGTG ATTGCTGGTT CTTCCAAGAT GAAGGGATTC 
TCATCCTCTG AGTCGGAGAG CACCAGCGAA TCCAGCTCCT CTGACAGTGA AGACTCTGAA 
ACAGAGATGG CTCCCAAGTC AAAAAAGAAG GGGCACACTG GGAGGGACCA GAAAAAGCAT 
CATCACCATC ACCATCCACA GATGCAGCCA GCCCCAGCTC CTGTGCCCCA GCAGCCGCCC 
CCACCTCCAC AGCAGCCTCC TCCACCCCCA CCTCCGCAGC AGCAGCAGCA GCAACCTCCA 
CCCCCACCAC CTCCGCCCTC CATGCCACAG CAGACTGCCC CAGCGATGAA GTCCTCGCCC 
CCACCCTTCA TCACTGCCCA GGTCCCCGTC CTGGAACCAC AGCTGCCAGG CAGTGTCTTT 
GACCCTATTG GCCACTTCAC CCAGCCCATC TTGCACCTGC CGCAGCCGGA GCTGCCTCCT 
CACCTGCCCC AGCCACCTGA GCACAGCACT CCACCCCATC TCAACCAGCA TGCTGTGGTC 
TCTCCTCCAG CTTTGCACAA TGCGCTGCCC CAACAGCCAT CTCGGCCCAG TAACCGAGCT 
GCTGCTCTGC CCCCAAAGCC TACCCGACCC CCAGCTGTGT CCCCTGCCCT GGCCCAGCCC 
CCCCTGCTCC CACAACCACC AATGGCTCAG CCCCCCCAAG TGCTGCTGGA GGATGAAGAG 
CCACCTGCCC CACCCCTCAC CTCCATGCAG ATGCAGTTGT ACCTACAGCA GCTGCAGAAG 
GTGCAGCCTC CCACACCACT ACTCCCTTCC GTGAAGGTGC AGTCCCAACC CCCACCGCCT 
TTGCCGCCTC CACCCCACCC TTCTGTGCAG CAGCAGCAGC TCCAGCCACA GCCACCGCCA 
CCCCCACCTC CTCAGCCACA GCCACCACCC CAGCAACAAC ACCAGCCTCC ACCACGACCA 
GTTCACTTGC CATCCATGCC CTTTTCTGCT CATATTCAGC AGCCCCCACC ACCCCCAGGA 
CAGCAACCTA CTCACCCACC CCCAGGACAG CAGCCCCCAC CACCACAGCC TGCCAAGCCC 
CAGCAAGTCA TCCAGCATCA CCCTTCCCCC CGGCACCACA AGTCAGACCC CTACTCAGCT 
GGTCATCTTC GTGAGGCTCC CTCTCCGCTT ATGATACATT CCCCTCAGAT GCCACAGTTC 
CAGAGCCTGA CCCATCAGTC TCCTCCCCAG CAAAACGTCC AGCCTAAGAA GCAGGTAAAG 
GGCAGGGCTG AGCCACAGCC ACCAGGGCCA GTCATGGGCC AAGGCCAGGG ATGCCCACCT 
GCCTCACCGG CTGCCGTGCC TATGCTGTCC CAGGAGCTGC GACCACCTTC AGTCGTCCAG 
CCCCAGCCCC TGGTGGTAGT AAAGGAGGAG AAGATTCACT CACCAATCAT TCGCAGCGAG 
CCTTTCAGCA CCTCACTTCG ACCAGAGCCC CCCAAGCACC CAGAGAACAT CAAGGCCCCA 
GTCCACCTGC CCCAGCGGCC TGAGATGAAG CCTGTAGACA TAGGGAGGCC TGTGATCCGG 
CCTCCAGAGC AGAGTGCACC CCCACCAGGG GCCCCTGACA AGGACAAACA GAAGCAGGAG 
CCAAAAACAC CAGTGGCACC CAAAAAGGAC CTGAAAATTA AGAACATGGG CTCCTGGGCC 
AGCCTGGTAC AGAAGCATCC GACCACCCCA TCCTCCACAG CCAAGTCCTC AAGTGACAGC 
TTTGAGCATT TCCGCCGTGC TGCTCGGGAG AAGGAGGAGA GGGAAAAGGC CCTGAAGGCT 
CAGGCTGAGC ATGCAGAGAA GGAGAAGGAG CGGCTGAGGC AGGAGCGCAT GAGGAGCCGA 
GAGGATGAAG ATGCGCTGGA ACAGGCACGT CGAGCCCATG AGGAGGCACG ACGGCGCCAG 
GAGCAGCAGC AGCAGCAGCA GCAGCAGAGA CAGGAGCAGC AGCAGCAGCA GCAGCAGGCA 
GCTGCTGTGG CCGCTGCCTC TGCCCCCCAG GCCCAGAGCT CCCAGCCCCA GTCTATGCTG 
GACCAGCAGA GGGAGTTGGC CCGGAAACGA GAGCAGGAGC GGAGGCGCAG GGAGGCAATG 
GCAGCTACAA TTGACATGAA TTTCCAGAGT GATCTTTTGT CAATATTTGA AGAAAATCTT 
TTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524750.1
CDS	124..4329
Translation	MSTESGPGTRLRNLPVMGDGLETSQMSTTQAQAQPQPANAASTNPPPPETSNPNKPKRQTNQLQYLLRVVLKTLWKHQFAWPFQQPVDAVKLNLPDYYKIIKTPMDMGTIKKRLENNYYWNAQECIQDFNTMFTNCYIYNKPGDDIVLMAEALEKLFLQKINELPTEETEIMIVQAKGRGRGRKETGRAAKPGVSTVPNTTQASTSPQTQTPQQNPPPPVQATTHPFPAVTPDLIAQPPVMTMVPPQPLQTPSPVPPQPPPPPAPVPQPVQSHPPIIATTPQPVKTKKGVKRKADTTTPTTIDPIHEPPSLAPEPKTAKLGPRRESSRPVKPPKKDVPDSQQHPGPEKSSKISEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYCDIIKHPMDMSTIKSKLESREYRDAQEFGADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPEEPVVTVSSPAVPPPTKVVAPPSSSDSSSDSSSDSDSSTDDSEEERAQRLAELQEQLKAVHEQLAALSQPQQNKPKKKEKDKKEKKKEKHKKKEEVEENKKSKTKELPPKKTKKNNSSNSNVSKKEPVPTKTKPPPTYESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVDVIAGSSKMKGFSSSESESTSESSSSDSEDSETEMAPKSKKKGHTGRDQKKHHHHHHPQMQPAPAPVPQQPPPPPQQPPPPPPPQQQQQQPPPPPPPPSMPQQTAPAMKSSPPPFITAQVPVLEPQLPGSVFDPIGHFTQPILHLPQPELPPHLPQPPEHSTPPHLNQHAVVSPPALHNALPQQPSRPSNRAAALPPKPTRPPAVSPALAQPPLLPQPPMAQPPQVLLEDEEPPAPPLTSMQMQLYLQQLQKVQPPTPLLPSVKVQSQPPPPLPPPPHPSVQQQQLQPQPPPPPPPQPQPPPQQQHQPPPRPVHLPSMPFSAHIQQPPPPPGQQPTHPPPGQQPPPPQPAKPQQVIQHHPSPRHHKSDPYSAGHLREAPSPLMIHSPQMPQFQSLTHQSPPQQNVQPKKQVKGRAEPQPPGPVMGQGQGCPPASPAAVPMLSQELRPPSVVQPQPLVVVKEEKIHSPIIRSEPFSTSLRPEPPKHPENIKAPVHLPQRPEMKPVDIGRPVIRPPEQSAPPPGAPDKDKQKQEPKTPVAPKKDLKIKNMGSWASLVQKHPTTPSSTAKSSSDSFEHFRRAAREKEEREKALKAQAEHAEKEKERLRQERMRSREDEDALEQARRAHEEARRRQEQQQQQQQQRQEQQQQQQQAAAVAAASAPQAQSSQPQSMLDQQRELARKREQERRRREAMAATIDMNFQSDLLSIFEENLF

Target ORF information:

RefSeq Version	XM_006524687.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus bromodomain containing 4 (Brd4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524687.2

ORF Insert Sequence:

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGCC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGACAGACA 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCCGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAAAAA 
ATCAATGAAC TGCCTACAGA AGAAACTGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGAGGGAGGA AAGAAACAGG TAGGGCAGCA AAGCCTGGTG TATCCACGGT ACCAAACACA 
ACTCAAGCAT CAACTTCTCC GCAGACCCAG ACGCCTCAGC AGAACCCTCC TCCACCTGTG 
CAGGCCACAA CTCACCCCTT TCCTGCTGTC ACCCCAGACC TCATTGCCCA GCCTCCTGTC 
ATGACAATGG TGCCCCCTCA GCCACTTCAG ACTCCTTCAC CGGTACCCCC CCAGCCACCA 
CCCCCACCTG CTCCAGTTCC ACAGCCTGTG CAGAGTCACC CGCCCATCAT TGCGACCACC 
CCCCAGCCTG TGAAGACAAA GAAAGGGGTG AAGAGGAAAG CAGATACCAC CACCCCTACC 
ACCATCGACC CCATTCATGA GCCACCCTCA CTGGCCCCAG AGCCCAAGAC CGCCAAGCTG 
GGTCCTCGGC GGGAGAGCAG CAGACCTGTG AAGCCTCCAA AGAAGGATGT ACCGGACTCA 
CAGCAGCACC CAGGGCCAGA GAAGAGCAGC AAGATCTCTG AGCAGCTAAA GTGCTGCAGT 
GGCATCCTCA AGGAGATGTT TGCCAAGAAA CATGCTGCCT ATGCCTGGCC TTTCTACAAG 
CCTGTGGATG TGGAGGCACT GGGTCTGCAC GACTACTGTG ACATCATCAA ACATCCCATG 
GACATGAGCA CAATCAAGTC TAAACTAGAG TCCCGAGAGT ACAGAGATGC CCAGGAATTT 
GGTGCTGATG TCCGATTGAT GTTCTCCAAC TGCTACAAGT ACAACCCCCC TGACCATGAA 
GTGGTAGCCA TGGCTCGAAA ACTCCAGGAT GTGTTTGAAA TGCGCTTTGC CAAGATGCCT 
GATGAGCCTG AAGAGCCAGT TGTTACAGTG TCCTCTCCTG CAGTGCCACC CCCTACAAAG 
GTGGTAGCCC CACCCTCATC TAGTGACAGC AGCAGCGACA GTTCTTCCGA CAGCGACAGT 
TCCACTGACG ACTCTGAGGA AGAGCGAGCC CAGCGGCTGG CTGAACTCCA GGAACAGCTC 
AAGGCCGTGC ATGAGCAGCT TGCAGCCCTC TCACAGCCCC AGCAGAACAA ACCAAAGAAA 
AAGGAGAAGG ACAAGAAGGA AAAGAAAAAG GAAAAGCACA AAAAGAAAGA AGAAGTGGAG 
GAAAATAAAA AAAGCAAAAC CAAGGAACTT CCTCCCAAAA AGACAAAGAA AAATAACAGC 
AGCAACAGCA ATGTGAGCAA GAAGGAACCA GTACCCACGA AGACCAAGCC GCCTCCCACA 
TATGAATCAG AAGAGGAGGA TAAGTGTAAG CCCATGTCTT ATGAGGAGAA GCGGCAGCTA 
AGTCTAGATA TCAACAAACT TCCTGGTGAG AAGCTAGGCC GTGTAGTACA CATAATTCAG 
TCAAGGGAAC CATCACTTAA AAACTCCAAC CCCGATGAGA TTGAGATTGA CTTTGAGACC 
CTGAAGCCAT CTACACTACG AGAGTTGGAG CGATATGTCA CCTCCTGTTT GCGGAAGAAA 
AGGAAACCTC AAGCTGAAAA AGTTGACGTG ATTGCTGGTT CTTCCAAGAT GAAGGGATTC 
TCATCCTCTG AGTCGGAGAG CACCAGCGAA TCCAGCTCCT CTGACAGTGA AGACTCTGAA 
ACAGAGATGG CTCCCAAGTC AAAAAAGAAG GGGCACACTG GGAGGGACCA GAAAAAGCAT 
CATCACCATC ACCATCCACA GATGCAGCCA GCCCCAGCTC CTGTGCCCCA GCAGCCGCCC 
CCACCTCCAC AGCAGCCTCC TCCACCCCCA CCTCCGCAGC AGCAGCAGCA GCAACCTCCA 
CCCCCACCAC CTCCGCCCTC CATGCCACAG CAGACTGCCC CAGCGATGAA GTCCTCGCCC 
CCACCCTTCA TCACTGCCCA GGTCCCCGTC CTGGAACCAC AGCTGCCAGG CAGTGTCTTT 
GACCCTATTG GCCACTTCAC CCAGCCCATC TTGCACCTGC CGCAGCCGGA GCTGCCTCCT 
CACCTGCCCC AGCCACCTGA GCACAGCACT CCACCCCATC TCAACCAGCA TGCTGTGGTC 
TCTCCTCCAG CTTTGCACAA TGCGCTGCCC CAACAGCCAT CTCGGCCCAG TAACCGAGCT 
GCTGCTCTGC CCCCAAAGCC TACCCGACCC CCAGCTGTGT CCCCTGCCCT GGCCCAGCCC 
CCCCTGCTCC CACAACCACC AATGGCTCAG CCCCCCCAAG TGCTGCTGGA GGATGAAGAG 
CCACCTGCCC CACCCCTCAC CTCCATGCAG ATGCAGTTGT ACCTACAGCA GCTGCAGAAG 
GTGCAGCCTC CCACACCACT ACTCCCTTCC GTGAAGGTGC AGTCCCAACC CCCACCGCCT 
TTGCCGCCTC CACCCCACCC TTCTGTGCAG CAGCAGCAGC TCCAGCCACA GCCACCGCCA 
CCCCCACCTC CTCAGCCACA GCCACCACCC CAGCAACAAC ACCAGCCTCC ACCACGACCA 
GTTCACTTGC CATCCATGCC CTTTTCTGCT CATATTCAGC AGCCCCCACC ACCCCCAGGA 
CAGCAACCTA CTCACCCACC CCCAGGACAG CAGCCCCCAC CACCACAGCC TGCCAAGCCC 
CAGCAAGTCA TCCAGCATCA CCCTTCCCCC CGGCACCACA AGTCAGACCC CTACTCAGCT 
GGTCATCTTC GTGAGGCTCC CTCTCCGCTT ATGATACATT CCCCTCAGAT GCCACAGTTC 
CAGAGCCTGA CCCATCAGTC TCCTCCCCAG CAAAACGTCC AGCCTAAGAA GCAGGTAAAG 
GGCAGGGCTG AGCCACAGCC ACCAGGGCCA GTCATGGGCC AAGGCCAGGG ATGCCCACCT 
GCCTCACCGG CTGCCGTGCC TATGCTGTCC CAGGAGCTGC GACCACCTTC AGTCGTCCAG 
CCCCAGCCCC TGGTGGTAGT AAAGGAGGAG AAGATTCACT CACCAATCAT TCGCAGCGAG 
CCTTTCAGCA CCTCACTTCG ACCAGAGCCC CCCAAGCACC CAGAGAACAT CAAGGCCCCA 
GTCCACCTGC CCCAGCGGCC TGAGATGAAG CCTGTAGACA TAGGGAGGCC TGTGATCCGG 
CCTCCAGAGC AGAGTGCACC CCCACCAGGG GCCCCTGACA AGGACAAACA GAAGCAGGAG 
CCAAAAACAC CAGTGGCACC CAAAAAGGAC CTGAAAATTA AGAACATGGG CTCCTGGGCC 
AGCCTGGTAC AGAAGCATCC GACCACCCCA TCCTCCACAG CCAAGTCCTC AAGTGACAGC 
TTTGAGCATT TCCGCCGTGC TGCTCGGGAG AAGGAGGAGA GGGAAAAGGC CCTGAAGGCT 
CAGGCTGAGC ATGCAGAGAA GGAGAAGGAG CGGCTGAGGC AGGAGCGCAT GAGGAGCCGA 
GAGGATGAAG ATGCGCTGGA ACAGGCACGT CGAGCCCATG AGGAGGCACG ACGGCGCCAG 
GAGCAGCAGC AGCAGCAGCA GCAGCAGAGA CAGGAGCAGC AGCAGCAGCA GCAGCAGGCA 
GCTGCTGTGG CCGCTGCCTC TGCCCCCCAG GCCCAGAGCT CCCAGCCCCA GTCTATGCTG 
GACCAGCAGA GGGAGTTGGC CCGGAAACGA GAGCAGGAGC GGAGGCGCAG GGAGGCAATG 
GCAGCTACAA TTGACATGAA TTTCCAGAGT GATCTTTTGT CAATATTTGA AGAAAATCTT 
TTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brd4 ( XM_006524685.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524748.1 Mus musculus bromodomain containing 4 (Brd4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu17143
Clone ID Related Accession (Same CDS sequence)	XM_006524683.2 , XM_006524683.3 , XM_006524684.2 , XM_030249922.1 , XM_006524686.2 , XM_006524687.2 , XM_006524685.2 , XM_030249923.1 , NM_001286630.1 , XM_006524685.1 , XM_006524686.1 , XM_006524684.3
Accession Version	XM_006524685.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4206bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	bromodomain-containing protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006524685.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGCC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGACAGACA 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCCGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAAAAA 
ATCAATGAAC TGCCTACAGA AGAAACTGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGAGGGAGGA AAGAAACAGG TAGGGCAGCA AAGCCTGGTG TATCCACGGT ACCAAACACA 
ACTCAAGCAT CAACTTCTCC GCAGACCCAG ACGCCTCAGC AGAACCCTCC TCCACCTGTG 
CAGGCCACAA CTCACCCCTT TCCTGCTGTC ACCCCAGACC TCATTGCCCA GCCTCCTGTC 
ATGACAATGG TGCCCCCTCA GCCACTTCAG ACTCCTTCAC CGGTACCCCC CCAGCCACCA 
CCCCCACCTG CTCCAGTTCC ACAGCCTGTG CAGAGTCACC CGCCCATCAT TGCGACCACC 
CCCCAGCCTG TGAAGACAAA GAAAGGGGTG AAGAGGAAAG CAGATACCAC CACCCCTACC 
ACCATCGACC CCATTCATGA GCCACCCTCA CTGGCCCCAG AGCCCAAGAC CGCCAAGCTG 
GGTCCTCGGC GGGAGAGCAG CAGACCTGTG AAGCCTCCAA AGAAGGATGT ACCGGACTCA 
CAGCAGCACC CAGGGCCAGA GAAGAGCAGC AAGATCTCTG AGCAGCTAAA GTGCTGCAGT 
GGCATCCTCA AGGAGATGTT TGCCAAGAAA CATGCTGCCT ATGCCTGGCC TTTCTACAAG 
CCTGTGGATG TGGAGGCACT GGGTCTGCAC GACTACTGTG ACATCATCAA ACATCCCATG 
GACATGAGCA CAATCAAGTC TAAACTAGAG TCCCGAGAGT ACAGAGATGC CCAGGAATTT 
GGTGCTGATG TCCGATTGAT GTTCTCCAAC TGCTACAAGT ACAACCCCCC TGACCATGAA 
GTGGTAGCCA TGGCTCGAAA ACTCCAGGAT GTGTTTGAAA TGCGCTTTGC CAAGATGCCT 
GATGAGCCTG AAGAGCCAGT TGTTACAGTG TCCTCTCCTG CAGTGCCACC CCCTACAAAG 
GTGGTAGCCC CACCCTCATC TAGTGACAGC AGCAGCGACA GTTCTTCCGA CAGCGACAGT 
TCCACTGACG ACTCTGAGGA AGAGCGAGCC CAGCGGCTGG CTGAACTCCA GGAACAGCTC 
AAGGCCGTGC ATGAGCAGCT TGCAGCCCTC TCACAGCCCC AGCAGAACAA ACCAAAGAAA 
AAGGAGAAGG ACAAGAAGGA AAAGAAAAAG GAAAAGCACA AAAAGAAAGA AGAAGTGGAG 
GAAAATAAAA AAAGCAAAAC CAAGGAACTT CCTCCCAAAA AGACAAAGAA AAATAACAGC 
AGCAACAGCA ATGTGAGCAA GAAGGAACCA GTACCCACGA AGACCAAGCC GCCTCCCACA 
TATGAATCAG AAGAGGAGGA TAAGTGTAAG CCCATGTCTT ATGAGGAGAA GCGGCAGCTA 
AGTCTAGATA TCAACAAACT TCCTGGTGAG AAGCTAGGCC GTGTAGTACA CATAATTCAG 
TCAAGGGAAC CATCACTTAA AAACTCCAAC CCCGATGAGA TTGAGATTGA CTTTGAGACC 
CTGAAGCCAT CTACACTACG AGAGTTGGAG CGATATGTCA CCTCCTGTTT GCGGAAGAAA 
AGGAAACCTC AAGCTGAAAA AGTTGACGTG ATTGCTGGTT CTTCCAAGAT GAAGGGATTC 
TCATCCTCTG AGTCGGAGAG CACCAGCGAA TCCAGCTCCT CTGACAGTGA AGACTCTGAA 
ACAGAGATGG CTCCCAAGTC AAAAAAGAAG GGGCACACTG GGAGGGACCA GAAAAAGCAT 
CATCACCATC ACCATCCACA GATGCAGCCA GCCCCAGCTC CTGTGCCCCA GCAGCCGCCC 
CCACCTCCAC AGCAGCCTCC TCCACCCCCA CCTCCGCAGC AGCAGCAGCA GCAACCTCCA 
CCCCCACCAC CTCCGCCCTC CATGCCACAG CAGACTGCCC CAGCGATGAA GTCCTCGCCC 
CCACCCTTCA TCACTGCCCA GGTCCCCGTC CTGGAACCAC AGCTGCCAGG CAGTGTCTTT 
GACCCTATTG GCCACTTCAC CCAGCCCATC TTGCACCTGC CGCAGCCGGA GCTGCCTCCT 
CACCTGCCCC AGCCACCTGA GCACAGCACT CCACCCCATC TCAACCAGCA TGCTGTGGTC 
TCTCCTCCAG CTTTGCACAA TGCGCTGCCC CAACAGCCAT CTCGGCCCAG TAACCGAGCT 
GCTGCTCTGC CCCCAAAGCC TACCCGACCC CCAGCTGTGT CCCCTGCCCT GGCCCAGCCC 
CCCCTGCTCC CACAACCACC AATGGCTCAG CCCCCCCAAG TGCTGCTGGA GGATGAAGAG 
CCACCTGCCC CACCCCTCAC CTCCATGCAG ATGCAGTTGT ACCTACAGCA GCTGCAGAAG 
GTGCAGCCTC CCACACCACT ACTCCCTTCC GTGAAGGTGC AGTCCCAACC CCCACCGCCT 
TTGCCGCCTC CACCCCACCC TTCTGTGCAG CAGCAGCAGC TCCAGCCACA GCCACCGCCA 
CCCCCACCTC CTCAGCCACA GCCACCACCC CAGCAACAAC ACCAGCCTCC ACCACGACCA 
GTTCACTTGC CATCCATGCC CTTTTCTGCT CATATTCAGC AGCCCCCACC ACCCCCAGGA 
CAGCAACCTA CTCACCCACC CCCAGGACAG CAGCCCCCAC CACCACAGCC TGCCAAGCCC 
CAGCAAGTCA TCCAGCATCA CCCTTCCCCC CGGCACCACA AGTCAGACCC CTACTCAGCT 
GGTCATCTTC GTGAGGCTCC CTCTCCGCTT ATGATACATT CCCCTCAGAT GCCACAGTTC 
CAGAGCCTGA CCCATCAGTC TCCTCCCCAG CAAAACGTCC AGCCTAAGAA GCAGGTAAAG 
GGCAGGGCTG AGCCACAGCC ACCAGGGCCA GTCATGGGCC AAGGCCAGGG ATGCCCACCT 
GCCTCACCGG CTGCCGTGCC TATGCTGTCC CAGGAGCTGC GACCACCTTC AGTCGTCCAG 
CCCCAGCCCC TGGTGGTAGT AAAGGAGGAG AAGATTCACT CACCAATCAT TCGCAGCGAG 
CCTTTCAGCA CCTCACTTCG ACCAGAGCCC CCCAAGCACC CAGAGAACAT CAAGGCCCCA 
GTCCACCTGC CCCAGCGGCC TGAGATGAAG CCTGTAGACA TAGGGAGGCC TGTGATCCGG 
CCTCCAGAGC AGAGTGCACC CCCACCAGGG GCCCCTGACA AGGACAAACA GAAGCAGGAG 
CCAAAAACAC CAGTGGCACC CAAAAAGGAC CTGAAAATTA AGAACATGGG CTCCTGGGCC 
AGCCTGGTAC AGAAGCATCC GACCACCCCA TCCTCCACAG CCAAGTCCTC AAGTGACAGC 
TTTGAGCATT TCCGCCGTGC TGCTCGGGAG AAGGAGGAGA GGGAAAAGGC CCTGAAGGCT 
CAGGCTGAGC ATGCAGAGAA GGAGAAGGAG CGGCTGAGGC AGGAGCGCAT GAGGAGCCGA 
GAGGATGAAG ATGCGCTGGA ACAGGCACGT CGAGCCCATG AGGAGGCACG ACGGCGCCAG 
GAGCAGCAGC AGCAGCAGCA GCAGCAGAGA CAGGAGCAGC AGCAGCAGCA GCAGCAGGCA 
GCTGCTGTGG CCGCTGCCTC TGCCCCCCAG GCCCAGAGCT CCCAGCCCCA GTCTATGCTG 
GACCAGCAGA GGGAGTTGGC CCGGAAACGA GAGCAGGAGC GGAGGCGCAG GGAGGCAATG 
GCAGCTACAA TTGACATGAA TTTCCAGAGT GATCTTTTGT CAATATTTGA AGAAAATCTT 
TTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524748.1
CDS	276..4481
Translation	MSTESGPGTRLRNLPVMGDGLETSQMSTTQAQAQPQPANAASTNPPPPETSNPNKPKRQTNQLQYLLRVVLKTLWKHQFAWPFQQPVDAVKLNLPDYYKIIKTPMDMGTIKKRLENNYYWNAQECIQDFNTMFTNCYIYNKPGDDIVLMAEALEKLFLQKINELPTEETEIMIVQAKGRGRGRKETGRAAKPGVSTVPNTTQASTSPQTQTPQQNPPPPVQATTHPFPAVTPDLIAQPPVMTMVPPQPLQTPSPVPPQPPPPPAPVPQPVQSHPPIIATTPQPVKTKKGVKRKADTTTPTTIDPIHEPPSLAPEPKTAKLGPRRESSRPVKPPKKDVPDSQQHPGPEKSSKISEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYCDIIKHPMDMSTIKSKLESREYRDAQEFGADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPEEPVVTVSSPAVPPPTKVVAPPSSSDSSSDSSSDSDSSTDDSEEERAQRLAELQEQLKAVHEQLAALSQPQQNKPKKKEKDKKEKKKEKHKKKEEVEENKKSKTKELPPKKTKKNNSSNSNVSKKEPVPTKTKPPPTYESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVDVIAGSSKMKGFSSSESESTSESSSSDSEDSETEMAPKSKKKGHTGRDQKKHHHHHHPQMQPAPAPVPQQPPPPPQQPPPPPPPQQQQQQPPPPPPPPSMPQQTAPAMKSSPPPFITAQVPVLEPQLPGSVFDPIGHFTQPILHLPQPELPPHLPQPPEHSTPPHLNQHAVVSPPALHNALPQQPSRPSNRAAALPPKPTRPPAVSPALAQPPLLPQPPMAQPPQVLLEDEEPPAPPLTSMQMQLYLQQLQKVQPPTPLLPSVKVQSQPPPPLPPPPHPSVQQQQLQPQPPPPPPPQPQPPPQQQHQPPPRPVHLPSMPFSAHIQQPPPPPGQQPTHPPPGQQPPPPQPAKPQQVIQHHPSPRHHKSDPYSAGHLREAPSPLMIHSPQMPQFQSLTHQSPPQQNVQPKKQVKGRAEPQPPGPVMGQGQGCPPASPAAVPMLSQELRPPSVVQPQPLVVVKEEKIHSPIIRSEPFSTSLRPEPPKHPENIKAPVHLPQRPEMKPVDIGRPVIRPPEQSAPPPGAPDKDKQKQEPKTPVAPKKDLKIKNMGSWASLVQKHPTTPSSTAKSSSDSFEHFRRAAREKEEREKALKAQAEHAEKEKERLRQERMRSREDEDALEQARRAHEEARRRQEQQQQQQQQRQEQQQQQQQAAAVAAASAPQAQSSQPQSMLDQQRELARKREQERRRREAMAATIDMNFQSDLLSIFEENLF

Target ORF information:

RefSeq Version	XM_006524685.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus bromodomain containing 4 (Brd4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524685.2

ORF Insert Sequence:

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGCC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGACAGACA 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCCGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAAAAA 
ATCAATGAAC TGCCTACAGA AGAAACTGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGAGGGAGGA AAGAAACAGG TAGGGCAGCA AAGCCTGGTG TATCCACGGT ACCAAACACA 
ACTCAAGCAT CAACTTCTCC GCAGACCCAG ACGCCTCAGC AGAACCCTCC TCCACCTGTG 
CAGGCCACAA CTCACCCCTT TCCTGCTGTC ACCCCAGACC TCATTGCCCA GCCTCCTGTC 
ATGACAATGG TGCCCCCTCA GCCACTTCAG ACTCCTTCAC CGGTACCCCC CCAGCCACCA 
CCCCCACCTG CTCCAGTTCC ACAGCCTGTG CAGAGTCACC CGCCCATCAT TGCGACCACC 
CCCCAGCCTG TGAAGACAAA GAAAGGGGTG AAGAGGAAAG CAGATACCAC CACCCCTACC 
ACCATCGACC CCATTCATGA GCCACCCTCA CTGGCCCCAG AGCCCAAGAC CGCCAAGCTG 
GGTCCTCGGC GGGAGAGCAG CAGACCTGTG AAGCCTCCAA AGAAGGATGT ACCGGACTCA 
CAGCAGCACC CAGGGCCAGA GAAGAGCAGC AAGATCTCTG AGCAGCTAAA GTGCTGCAGT 
GGCATCCTCA AGGAGATGTT TGCCAAGAAA CATGCTGCCT ATGCCTGGCC TTTCTACAAG 
CCTGTGGATG TGGAGGCACT GGGTCTGCAC GACTACTGTG ACATCATCAA ACATCCCATG 
GACATGAGCA CAATCAAGTC TAAACTAGAG TCCCGAGAGT ACAGAGATGC CCAGGAATTT 
GGTGCTGATG TCCGATTGAT GTTCTCCAAC TGCTACAAGT ACAACCCCCC TGACCATGAA 
GTGGTAGCCA TGGCTCGAAA ACTCCAGGAT GTGTTTGAAA TGCGCTTTGC CAAGATGCCT 
GATGAGCCTG AAGAGCCAGT TGTTACAGTG TCCTCTCCTG CAGTGCCACC CCCTACAAAG 
GTGGTAGCCC CACCCTCATC TAGTGACAGC AGCAGCGACA GTTCTTCCGA CAGCGACAGT 
TCCACTGACG ACTCTGAGGA AGAGCGAGCC CAGCGGCTGG CTGAACTCCA GGAACAGCTC 
AAGGCCGTGC ATGAGCAGCT TGCAGCCCTC TCACAGCCCC AGCAGAACAA ACCAAAGAAA 
AAGGAGAAGG ACAAGAAGGA AAAGAAAAAG GAAAAGCACA AAAAGAAAGA AGAAGTGGAG 
GAAAATAAAA AAAGCAAAAC CAAGGAACTT CCTCCCAAAA AGACAAAGAA AAATAACAGC 
AGCAACAGCA ATGTGAGCAA GAAGGAACCA GTACCCACGA AGACCAAGCC GCCTCCCACA 
TATGAATCAG AAGAGGAGGA TAAGTGTAAG CCCATGTCTT ATGAGGAGAA GCGGCAGCTA 
AGTCTAGATA TCAACAAACT TCCTGGTGAG AAGCTAGGCC GTGTAGTACA CATAATTCAG 
TCAAGGGAAC CATCACTTAA AAACTCCAAC CCCGATGAGA TTGAGATTGA CTTTGAGACC 
CTGAAGCCAT CTACACTACG AGAGTTGGAG CGATATGTCA CCTCCTGTTT GCGGAAGAAA 
AGGAAACCTC AAGCTGAAAA AGTTGACGTG ATTGCTGGTT CTTCCAAGAT GAAGGGATTC 
TCATCCTCTG AGTCGGAGAG CACCAGCGAA TCCAGCTCCT CTGACAGTGA AGACTCTGAA 
ACAGAGATGG CTCCCAAGTC AAAAAAGAAG GGGCACACTG GGAGGGACCA GAAAAAGCAT 
CATCACCATC ACCATCCACA GATGCAGCCA GCCCCAGCTC CTGTGCCCCA GCAGCCGCCC 
CCACCTCCAC AGCAGCCTCC TCCACCCCCA CCTCCGCAGC AGCAGCAGCA GCAACCTCCA 
CCCCCACCAC CTCCGCCCTC CATGCCACAG CAGACTGCCC CAGCGATGAA GTCCTCGCCC 
CCACCCTTCA TCACTGCCCA GGTCCCCGTC CTGGAACCAC AGCTGCCAGG CAGTGTCTTT 
GACCCTATTG GCCACTTCAC CCAGCCCATC TTGCACCTGC CGCAGCCGGA GCTGCCTCCT 
CACCTGCCCC AGCCACCTGA GCACAGCACT CCACCCCATC TCAACCAGCA TGCTGTGGTC 
TCTCCTCCAG CTTTGCACAA TGCGCTGCCC CAACAGCCAT CTCGGCCCAG TAACCGAGCT 
GCTGCTCTGC CCCCAAAGCC TACCCGACCC CCAGCTGTGT CCCCTGCCCT GGCCCAGCCC 
CCCCTGCTCC CACAACCACC AATGGCTCAG CCCCCCCAAG TGCTGCTGGA GGATGAAGAG 
CCACCTGCCC CACCCCTCAC CTCCATGCAG ATGCAGTTGT ACCTACAGCA GCTGCAGAAG 
GTGCAGCCTC CCACACCACT ACTCCCTTCC GTGAAGGTGC AGTCCCAACC CCCACCGCCT 
TTGCCGCCTC CACCCCACCC TTCTGTGCAG CAGCAGCAGC TCCAGCCACA GCCACCGCCA 
CCCCCACCTC CTCAGCCACA GCCACCACCC CAGCAACAAC ACCAGCCTCC ACCACGACCA 
GTTCACTTGC CATCCATGCC CTTTTCTGCT CATATTCAGC AGCCCCCACC ACCCCCAGGA 
CAGCAACCTA CTCACCCACC CCCAGGACAG CAGCCCCCAC CACCACAGCC TGCCAAGCCC 
CAGCAAGTCA TCCAGCATCA CCCTTCCCCC CGGCACCACA AGTCAGACCC CTACTCAGCT 
GGTCATCTTC GTGAGGCTCC CTCTCCGCTT ATGATACATT CCCCTCAGAT GCCACAGTTC 
CAGAGCCTGA CCCATCAGTC TCCTCCCCAG CAAAACGTCC AGCCTAAGAA GCAGGTAAAG 
GGCAGGGCTG AGCCACAGCC ACCAGGGCCA GTCATGGGCC AAGGCCAGGG ATGCCCACCT 
GCCTCACCGG CTGCCGTGCC TATGCTGTCC CAGGAGCTGC GACCACCTTC AGTCGTCCAG 
CCCCAGCCCC TGGTGGTAGT AAAGGAGGAG AAGATTCACT CACCAATCAT TCGCAGCGAG 
CCTTTCAGCA CCTCACTTCG ACCAGAGCCC CCCAAGCACC CAGAGAACAT CAAGGCCCCA 
GTCCACCTGC CCCAGCGGCC TGAGATGAAG CCTGTAGACA TAGGGAGGCC TGTGATCCGG 
CCTCCAGAGC AGAGTGCACC CCCACCAGGG GCCCCTGACA AGGACAAACA GAAGCAGGAG 
CCAAAAACAC CAGTGGCACC CAAAAAGGAC CTGAAAATTA AGAACATGGG CTCCTGGGCC 
AGCCTGGTAC AGAAGCATCC GACCACCCCA TCCTCCACAG CCAAGTCCTC AAGTGACAGC 
TTTGAGCATT TCCGCCGTGC TGCTCGGGAG AAGGAGGAGA GGGAAAAGGC CCTGAAGGCT 
CAGGCTGAGC ATGCAGAGAA GGAGAAGGAG CGGCTGAGGC AGGAGCGCAT GAGGAGCCGA 
GAGGATGAAG ATGCGCTGGA ACAGGCACGT CGAGCCCATG AGGAGGCACG ACGGCGCCAG 
GAGCAGCAGC AGCAGCAGCA GCAGCAGAGA CAGGAGCAGC AGCAGCAGCA GCAGCAGGCA 
GCTGCTGTGG CCGCTGCCTC TGCCCCCCAG GCCCAGAGCT CCCAGCCCCA GTCTATGCTG 
GACCAGCAGA GGGAGTTGGC CCGGAAACGA GAGCAGGAGC GGAGGCGCAG GGAGGCAATG 
GCAGCTACAA TTGACATGAA TTTCCAGAGT GATCTTTTGT CAATATTTGA AGAAAATCTT 
TTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brd4 ( XM_030249923.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105783.1 Mus musculus bromodomain containing 4 (Brd4), transcript variant X5, mRNA.

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CloneID	OMu17143
Clone ID Related Accession (Same CDS sequence)	XM_006524683.2 , XM_006524683.3 , XM_006524684.2 , XM_030249922.1 , XM_006524686.2 , XM_006524687.2 , XM_006524685.2 , XM_030249923.1 , NM_001286630.1 , XM_006524685.1 , XM_006524686.1 , XM_006524684.3
Accession Version	XM_030249923.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4206bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	bromodomain-containing protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGCC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGACAGACA 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCCGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAAAAA 
ATCAATGAAC TGCCTACAGA AGAAACTGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGAGGGAGGA AAGAAACAGG TAGGGCAGCA AAGCCTGGTG TATCCACGGT ACCAAACACA 
ACTCAAGCAT CAACTTCTCC GCAGACCCAG ACGCCTCAGC AGAACCCTCC TCCACCTGTG 
CAGGCCACAA CTCACCCCTT TCCTGCTGTC ACCCCAGACC TCATTGCCCA GCCTCCTGTC 
ATGACAATGG TGCCCCCTCA GCCACTTCAG ACTCCTTCAC CGGTACCCCC CCAGCCACCA 
CCCCCACCTG CTCCAGTTCC ACAGCCTGTG CAGAGTCACC CGCCCATCAT TGCGACCACC 
CCCCAGCCTG TGAAGACAAA GAAAGGGGTG AAGAGGAAAG CAGATACCAC CACCCCTACC 
ACCATCGACC CCATTCATGA GCCACCCTCA CTGGCCCCAG AGCCCAAGAC CGCCAAGCTG 
GGTCCTCGGC GGGAGAGCAG CAGACCTGTG AAGCCTCCAA AGAAGGATGT ACCGGACTCA 
CAGCAGCACC CAGGGCCAGA GAAGAGCAGC AAGATCTCTG AGCAGCTAAA GTGCTGCAGT 
GGCATCCTCA AGGAGATGTT TGCCAAGAAA CATGCTGCCT ATGCCTGGCC TTTCTACAAG 
CCTGTGGATG TGGAGGCACT GGGTCTGCAC GACTACTGTG ACATCATCAA ACATCCCATG 
GACATGAGCA CAATCAAGTC TAAACTAGAG TCCCGAGAGT ACAGAGATGC CCAGGAATTT 
GGTGCTGATG TCCGATTGAT GTTCTCCAAC TGCTACAAGT ACAACCCCCC TGACCATGAA 
GTGGTAGCCA TGGCTCGAAA ACTCCAGGAT GTGTTTGAAA TGCGCTTTGC CAAGATGCCT 
GATGAGCCTG AAGAGCCAGT TGTTACAGTG TCCTCTCCTG CAGTGCCACC CCCTACAAAG 
GTGGTAGCCC CACCCTCATC TAGTGACAGC AGCAGCGACA GTTCTTCCGA CAGCGACAGT 
TCCACTGACG ACTCTGAGGA AGAGCGAGCC CAGCGGCTGG CTGAACTCCA GGAACAGCTC 
AAGGCCGTGC ATGAGCAGCT TGCAGCCCTC TCACAGCCCC AGCAGAACAA ACCAAAGAAA 
AAGGAGAAGG ACAAGAAGGA AAAGAAAAAG GAAAAGCACA AAAAGAAAGA AGAAGTGGAG 
GAAAATAAAA AAAGCAAAAC CAAGGAACTT CCTCCCAAAA AGACAAAGAA AAATAACAGC 
AGCAACAGCA ATGTGAGCAA GAAGGAACCA GTACCCACGA AGACCAAGCC GCCTCCCACA 
TATGAATCAG AAGAGGAGGA TAAGTGTAAG CCCATGTCTT ATGAGGAGAA GCGGCAGCTA 
AGTCTAGATA TCAACAAACT TCCTGGTGAG AAGCTAGGCC GTGTAGTACA CATAATTCAG 
TCAAGGGAAC CATCACTTAA AAACTCCAAC CCCGATGAGA TTGAGATTGA CTTTGAGACC 
CTGAAGCCAT CTACACTACG AGAGTTGGAG CGATATGTCA CCTCCTGTTT GCGGAAGAAA 
AGGAAACCTC AAGCTGAAAA AGTTGACGTG ATTGCTGGTT CTTCCAAGAT GAAGGGATTC 
TCATCCTCTG AGTCGGAGAG CACCAGCGAA TCCAGCTCCT CTGACAGTGA AGACTCTGAA 
ACAGAGATGG CTCCCAAGTC AAAAAAGAAG GGGCACACTG GGAGGGACCA GAAAAAGCAT 
CATCACCATC ACCATCCACA GATGCAGCCA GCCCCAGCTC CTGTGCCCCA GCAGCCGCCC 
CCACCTCCAC AGCAGCCTCC TCCACCCCCA CCTCCGCAGC AGCAGCAGCA GCAACCTCCA 
CCCCCACCAC CTCCGCCCTC CATGCCACAG CAGACTGCCC CAGCGATGAA GTCCTCGCCC 
CCACCCTTCA TCACTGCCCA GGTCCCCGTC CTGGAACCAC AGCTGCCAGG CAGTGTCTTT 
GACCCTATTG GCCACTTCAC CCAGCCCATC TTGCACCTGC CGCAGCCGGA GCTGCCTCCT 
CACCTGCCCC AGCCACCTGA GCACAGCACT CCACCCCATC TCAACCAGCA TGCTGTGGTC 
TCTCCTCCAG CTTTGCACAA TGCGCTGCCC CAACAGCCAT CTCGGCCCAG TAACCGAGCT 
GCTGCTCTGC CCCCAAAGCC TACCCGACCC CCAGCTGTGT CCCCTGCCCT GGCCCAGCCC 
CCCCTGCTCC CACAACCACC AATGGCTCAG CCCCCCCAAG TGCTGCTGGA GGATGAAGAG 
CCACCTGCCC CACCCCTCAC CTCCATGCAG ATGCAGTTGT ACCTACAGCA GCTGCAGAAG 
GTGCAGCCTC CCACACCACT ACTCCCTTCC GTGAAGGTGC AGTCCCAACC CCCACCGCCT 
TTGCCGCCTC CACCCCACCC TTCTGTGCAG CAGCAGCAGC TCCAGCCACA GCCACCGCCA 
CCCCCACCTC CTCAGCCACA GCCACCACCC CAGCAACAAC ACCAGCCTCC ACCACGACCA 
GTTCACTTGC CATCCATGCC CTTTTCTGCT CATATTCAGC AGCCCCCACC ACCCCCAGGA 
CAGCAACCTA CTCACCCACC CCCAGGACAG CAGCCCCCAC CACCACAGCC TGCCAAGCCC 
CAGCAAGTCA TCCAGCATCA CCCTTCCCCC CGGCACCACA AGTCAGACCC CTACTCAGCT 
GGTCATCTTC GTGAGGCTCC CTCTCCGCTT ATGATACATT CCCCTCAGAT GCCACAGTTC 
CAGAGCCTGA CCCATCAGTC TCCTCCCCAG CAAAACGTCC AGCCTAAGAA GCAGGTAAAG 
GGCAGGGCTG AGCCACAGCC ACCAGGGCCA GTCATGGGCC AAGGCCAGGG ATGCCCACCT 
GCCTCACCGG CTGCCGTGCC TATGCTGTCC CAGGAGCTGC GACCACCTTC AGTCGTCCAG 
CCCCAGCCCC TGGTGGTAGT AAAGGAGGAG AAGATTCACT CACCAATCAT TCGCAGCGAG 
CCTTTCAGCA CCTCACTTCG ACCAGAGCCC CCCAAGCACC CAGAGAACAT CAAGGCCCCA 
GTCCACCTGC CCCAGCGGCC TGAGATGAAG CCTGTAGACA TAGGGAGGCC TGTGATCCGG 
CCTCCAGAGC AGAGTGCACC CCCACCAGGG GCCCCTGACA AGGACAAACA GAAGCAGGAG 
CCAAAAACAC CAGTGGCACC CAAAAAGGAC CTGAAAATTA AGAACATGGG CTCCTGGGCC 
AGCCTGGTAC AGAAGCATCC GACCACCCCA TCCTCCACAG CCAAGTCCTC AAGTGACAGC 
TTTGAGCATT TCCGCCGTGC TGCTCGGGAG AAGGAGGAGA GGGAAAAGGC CCTGAAGGCT 
CAGGCTGAGC ATGCAGAGAA GGAGAAGGAG CGGCTGAGGC AGGAGCGCAT GAGGAGCCGA 
GAGGATGAAG ATGCGCTGGA ACAGGCACGT CGAGCCCATG AGGAGGCACG ACGGCGCCAG 
GAGCAGCAGC AGCAGCAGCA GCAGCAGAGA CAGGAGCAGC AGCAGCAGCA GCAGCAGGCA 
GCTGCTGTGG CCGCTGCCTC TGCCCCCCAG GCCCAGAGCT CCCAGCCCCA GTCTATGCTG 
GACCAGCAGA GGGAGTTGGC CCGGAAACGA GAGCAGGAGC GGAGGCGCAG GGAGGCAATG 
GCAGCTACAA TTGACATGAA TTTCCAGAGT GATCTTTTGT CAATATTTGA AGAAAATCTT 
TTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105783.1
CDS	478..4683
Translation	MSTESGPGTRLRNLPVMGDGLETSQMSTTQAQAQPQPANAASTNPPPPETSNPNKPKRQTNQLQYLLRVVLKTLWKHQFAWPFQQPVDAVKLNLPDYYKIIKTPMDMGTIKKRLENNYYWNAQECIQDFNTMFTNCYIYNKPGDDIVLMAEALEKLFLQKINELPTEETEIMIVQAKGRGRGRKETGRAAKPGVSTVPNTTQASTSPQTQTPQQNPPPPVQATTHPFPAVTPDLIAQPPVMTMVPPQPLQTPSPVPPQPPPPPAPVPQPVQSHPPIIATTPQPVKTKKGVKRKADTTTPTTIDPIHEPPSLAPEPKTAKLGPRRESSRPVKPPKKDVPDSQQHPGPEKSSKISEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYCDIIKHPMDMSTIKSKLESREYRDAQEFGADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPEEPVVTVSSPAVPPPTKVVAPPSSSDSSSDSSSDSDSSTDDSEEERAQRLAELQEQLKAVHEQLAALSQPQQNKPKKKEKDKKEKKKEKHKKKEEVEENKKSKTKELPPKKTKKNNSSNSNVSKKEPVPTKTKPPPTYESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVDVIAGSSKMKGFSSSESESTSESSSSDSEDSETEMAPKSKKKGHTGRDQKKHHHHHHPQMQPAPAPVPQQPPPPPQQPPPPPPPQQQQQQPPPPPPPPSMPQQTAPAMKSSPPPFITAQVPVLEPQLPGSVFDPIGHFTQPILHLPQPELPPHLPQPPEHSTPPHLNQHAVVSPPALHNALPQQPSRPSNRAAALPPKPTRPPAVSPALAQPPLLPQPPMAQPPQVLLEDEEPPAPPLTSMQMQLYLQQLQKVQPPTPLLPSVKVQSQPPPPLPPPPHPSVQQQQLQPQPPPPPPPQPQPPPQQQHQPPPRPVHLPSMPFSAHIQQPPPPPGQQPTHPPPGQQPPPPQPAKPQQVIQHHPSPRHHKSDPYSAGHLREAPSPLMIHSPQMPQFQSLTHQSPPQQNVQPKKQVKGRAEPQPPGPVMGQGQGCPPASPAAVPMLSQELRPPSVVQPQPLVVVKEEKIHSPIIRSEPFSTSLRPEPPKHPENIKAPVHLPQRPEMKPVDIGRPVIRPPEQSAPPPGAPDKDKQKQEPKTPVAPKKDLKIKNMGSWASLVQKHPTTPSSTAKSSSDSFEHFRRAAREKEEREKALKAQAEHAEKEKERLRQERMRSREDEDALEQARRAHEEARRRQEQQQQQQQQRQEQQQQQQQAAAVAAASAPQAQSSQPQSMLDQQRELARKREQERRRREAMAATIDMNFQSDLLSIFEENLF

Target ORF information:

RefSeq Version	XM_030249923.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus bromodomain containing 4 (Brd4), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249923.1

ORF Insert Sequence:

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGCC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGACAGACA 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCCGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAAAAA 
ATCAATGAAC TGCCTACAGA AGAAACTGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGAGGGAGGA AAGAAACAGG TAGGGCAGCA AAGCCTGGTG TATCCACGGT ACCAAACACA 
ACTCAAGCAT CAACTTCTCC GCAGACCCAG ACGCCTCAGC AGAACCCTCC TCCACCTGTG 
CAGGCCACAA CTCACCCCTT TCCTGCTGTC ACCCCAGACC TCATTGCCCA GCCTCCTGTC 
ATGACAATGG TGCCCCCTCA GCCACTTCAG ACTCCTTCAC CGGTACCCCC CCAGCCACCA 
CCCCCACCTG CTCCAGTTCC ACAGCCTGTG CAGAGTCACC CGCCCATCAT TGCGACCACC 
CCCCAGCCTG TGAAGACAAA GAAAGGGGTG AAGAGGAAAG CAGATACCAC CACCCCTACC 
ACCATCGACC CCATTCATGA GCCACCCTCA CTGGCCCCAG AGCCCAAGAC CGCCAAGCTG 
GGTCCTCGGC GGGAGAGCAG CAGACCTGTG AAGCCTCCAA AGAAGGATGT ACCGGACTCA 
CAGCAGCACC CAGGGCCAGA GAAGAGCAGC AAGATCTCTG AGCAGCTAAA GTGCTGCAGT 
GGCATCCTCA AGGAGATGTT TGCCAAGAAA CATGCTGCCT ATGCCTGGCC TTTCTACAAG 
CCTGTGGATG TGGAGGCACT GGGTCTGCAC GACTACTGTG ACATCATCAA ACATCCCATG 
GACATGAGCA CAATCAAGTC TAAACTAGAG TCCCGAGAGT ACAGAGATGC CCAGGAATTT 
GGTGCTGATG TCCGATTGAT GTTCTCCAAC TGCTACAAGT ACAACCCCCC TGACCATGAA 
GTGGTAGCCA TGGCTCGAAA ACTCCAGGAT GTGTTTGAAA TGCGCTTTGC CAAGATGCCT 
GATGAGCCTG AAGAGCCAGT TGTTACAGTG TCCTCTCCTG CAGTGCCACC CCCTACAAAG 
GTGGTAGCCC CACCCTCATC TAGTGACAGC AGCAGCGACA GTTCTTCCGA CAGCGACAGT 
TCCACTGACG ACTCTGAGGA AGAGCGAGCC CAGCGGCTGG CTGAACTCCA GGAACAGCTC 
AAGGCCGTGC ATGAGCAGCT TGCAGCCCTC TCACAGCCCC AGCAGAACAA ACCAAAGAAA 
AAGGAGAAGG ACAAGAAGGA AAAGAAAAAG GAAAAGCACA AAAAGAAAGA AGAAGTGGAG 
GAAAATAAAA AAAGCAAAAC CAAGGAACTT CCTCCCAAAA AGACAAAGAA AAATAACAGC 
AGCAACAGCA ATGTGAGCAA GAAGGAACCA GTACCCACGA AGACCAAGCC GCCTCCCACA 
TATGAATCAG AAGAGGAGGA TAAGTGTAAG CCCATGTCTT ATGAGGAGAA GCGGCAGCTA 
AGTCTAGATA TCAACAAACT TCCTGGTGAG AAGCTAGGCC GTGTAGTACA CATAATTCAG 
TCAAGGGAAC CATCACTTAA AAACTCCAAC CCCGATGAGA TTGAGATTGA CTTTGAGACC 
CTGAAGCCAT CTACACTACG AGAGTTGGAG CGATATGTCA CCTCCTGTTT GCGGAAGAAA 
AGGAAACCTC AAGCTGAAAA AGTTGACGTG ATTGCTGGTT CTTCCAAGAT GAAGGGATTC 
TCATCCTCTG AGTCGGAGAG CACCAGCGAA TCCAGCTCCT CTGACAGTGA AGACTCTGAA 
ACAGAGATGG CTCCCAAGTC AAAAAAGAAG GGGCACACTG GGAGGGACCA GAAAAAGCAT 
CATCACCATC ACCATCCACA GATGCAGCCA GCCCCAGCTC CTGTGCCCCA GCAGCCGCCC 
CCACCTCCAC AGCAGCCTCC TCCACCCCCA CCTCCGCAGC AGCAGCAGCA GCAACCTCCA 
CCCCCACCAC CTCCGCCCTC CATGCCACAG CAGACTGCCC CAGCGATGAA GTCCTCGCCC 
CCACCCTTCA TCACTGCCCA GGTCCCCGTC CTGGAACCAC AGCTGCCAGG CAGTGTCTTT 
GACCCTATTG GCCACTTCAC CCAGCCCATC TTGCACCTGC CGCAGCCGGA GCTGCCTCCT 
CACCTGCCCC AGCCACCTGA GCACAGCACT CCACCCCATC TCAACCAGCA TGCTGTGGTC 
TCTCCTCCAG CTTTGCACAA TGCGCTGCCC CAACAGCCAT CTCGGCCCAG TAACCGAGCT 
GCTGCTCTGC CCCCAAAGCC TACCCGACCC CCAGCTGTGT CCCCTGCCCT GGCCCAGCCC 
CCCCTGCTCC CACAACCACC AATGGCTCAG CCCCCCCAAG TGCTGCTGGA GGATGAAGAG 
CCACCTGCCC CACCCCTCAC CTCCATGCAG ATGCAGTTGT ACCTACAGCA GCTGCAGAAG 
GTGCAGCCTC CCACACCACT ACTCCCTTCC GTGAAGGTGC AGTCCCAACC CCCACCGCCT 
TTGCCGCCTC CACCCCACCC TTCTGTGCAG CAGCAGCAGC TCCAGCCACA GCCACCGCCA 
CCCCCACCTC CTCAGCCACA GCCACCACCC CAGCAACAAC ACCAGCCTCC ACCACGACCA 
GTTCACTTGC CATCCATGCC CTTTTCTGCT CATATTCAGC AGCCCCCACC ACCCCCAGGA 
CAGCAACCTA CTCACCCACC CCCAGGACAG CAGCCCCCAC CACCACAGCC TGCCAAGCCC 
CAGCAAGTCA TCCAGCATCA CCCTTCCCCC CGGCACCACA AGTCAGACCC CTACTCAGCT 
GGTCATCTTC GTGAGGCTCC CTCTCCGCTT ATGATACATT CCCCTCAGAT GCCACAGTTC 
CAGAGCCTGA CCCATCAGTC TCCTCCCCAG CAAAACGTCC AGCCTAAGAA GCAGGTAAAG 
GGCAGGGCTG AGCCACAGCC ACCAGGGCCA GTCATGGGCC AAGGCCAGGG ATGCCCACCT 
GCCTCACCGG CTGCCGTGCC TATGCTGTCC CAGGAGCTGC GACCACCTTC AGTCGTCCAG 
CCCCAGCCCC TGGTGGTAGT AAAGGAGGAG AAGATTCACT CACCAATCAT TCGCAGCGAG 
CCTTTCAGCA CCTCACTTCG ACCAGAGCCC CCCAAGCACC CAGAGAACAT CAAGGCCCCA 
GTCCACCTGC CCCAGCGGCC TGAGATGAAG CCTGTAGACA TAGGGAGGCC TGTGATCCGG 
CCTCCAGAGC AGAGTGCACC CCCACCAGGG GCCCCTGACA AGGACAAACA GAAGCAGGAG 
CCAAAAACAC CAGTGGCACC CAAAAAGGAC CTGAAAATTA AGAACATGGG CTCCTGGGCC 
AGCCTGGTAC AGAAGCATCC GACCACCCCA TCCTCCACAG CCAAGTCCTC AAGTGACAGC 
TTTGAGCATT TCCGCCGTGC TGCTCGGGAG AAGGAGGAGA GGGAAAAGGC CCTGAAGGCT 
CAGGCTGAGC ATGCAGAGAA GGAGAAGGAG CGGCTGAGGC AGGAGCGCAT GAGGAGCCGA 
GAGGATGAAG ATGCGCTGGA ACAGGCACGT CGAGCCCATG AGGAGGCACG ACGGCGCCAG 
GAGCAGCAGC AGCAGCAGCA GCAGCAGAGA CAGGAGCAGC AGCAGCAGCA GCAGCAGGCA 
GCTGCTGTGG CCGCTGCCTC TGCCCCCCAG GCCCAGAGCT CCCAGCCCCA GTCTATGCTG 
GACCAGCAGA GGGAGTTGGC CCGGAAACGA GAGCAGGAGC GGAGGCGCAG GGAGGCAATG 
GCAGCTACAA TTGACATGAA TTTCCAGAGT GATCTTTTGT CAATATTTGA AGAAAATCTT 
TTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brd4 ( XM_006524685.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524748.1 Mus musculus bromodomain containing 4 (Brd4), transcript variant X3, mRNA.

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CloneID	OMu17143
Clone ID Related Accession (Same CDS sequence)	XM_006524683.2 , XM_006524683.3 , XM_006524684.2 , XM_030249922.1 , XM_006524686.2 , XM_006524687.2 , XM_006524685.2 , XM_030249923.1 , NM_001286630.1 , XM_006524685.1 , XM_006524686.1 , XM_006524684.3
Accession Version	XM_006524685.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4206bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	bromodomain-containing protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGCC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGACAGACA 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCCGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAAAAA 
ATCAATGAAC TGCCTACAGA AGAAACTGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGAGGGAGGA AAGAAACAGG TAGGGCAGCA AAGCCTGGTG TATCCACGGT ACCAAACACA 
ACTCAAGCAT CAACTTCTCC GCAGACCCAG ACGCCTCAGC AGAACCCTCC TCCACCTGTG 
CAGGCCACAA CTCACCCCTT TCCTGCTGTC ACCCCAGACC TCATTGCCCA GCCTCCTGTC 
ATGACAATGG TGCCCCCTCA GCCACTTCAG ACTCCTTCAC CGGTACCCCC CCAGCCACCA 
CCCCCACCTG CTCCAGTTCC ACAGCCTGTG CAGAGTCACC CGCCCATCAT TGCGACCACC 
CCCCAGCCTG TGAAGACAAA GAAAGGGGTG AAGAGGAAAG CAGATACCAC CACCCCTACC 
ACCATCGACC CCATTCATGA GCCACCCTCA CTGGCCCCAG AGCCCAAGAC CGCCAAGCTG 
GGTCCTCGGC GGGAGAGCAG CAGACCTGTG AAGCCTCCAA AGAAGGATGT ACCGGACTCA 
CAGCAGCACC CAGGGCCAGA GAAGAGCAGC AAGATCTCTG AGCAGCTAAA GTGCTGCAGT 
GGCATCCTCA AGGAGATGTT TGCCAAGAAA CATGCTGCCT ATGCCTGGCC TTTCTACAAG 
CCTGTGGATG TGGAGGCACT GGGTCTGCAC GACTACTGTG ACATCATCAA ACATCCCATG 
GACATGAGCA CAATCAAGTC TAAACTAGAG TCCCGAGAGT ACAGAGATGC CCAGGAATTT 
GGTGCTGATG TCCGATTGAT GTTCTCCAAC TGCTACAAGT ACAACCCCCC TGACCATGAA 
GTGGTAGCCA TGGCTCGAAA ACTCCAGGAT GTGTTTGAAA TGCGCTTTGC CAAGATGCCT 
GATGAGCCTG AAGAGCCAGT TGTTACAGTG TCCTCTCCTG CAGTGCCACC CCCTACAAAG 
GTGGTAGCCC CACCCTCATC TAGTGACAGC AGCAGCGACA GTTCTTCCGA CAGCGACAGT 
TCCACTGACG ACTCTGAGGA AGAGCGAGCC CAGCGGCTGG CTGAACTCCA GGAACAGCTC 
AAGGCCGTGC ATGAGCAGCT TGCAGCCCTC TCACAGCCCC AGCAGAACAA ACCAAAGAAA 
AAGGAGAAGG ACAAGAAGGA AAAGAAAAAG GAAAAGCACA AAAAGAAAGA AGAAGTGGAG 
GAAAATAAAA AAAGCAAAAC CAAGGAACTT CCTCCCAAAA AGACAAAGAA AAATAACAGC 
AGCAACAGCA ATGTGAGCAA GAAGGAACCA GTACCCACGA AGACCAAGCC GCCTCCCACA 
TATGAATCAG AAGAGGAGGA TAAGTGTAAG CCCATGTCTT ATGAGGAGAA GCGGCAGCTA 
AGTCTAGATA TCAACAAACT TCCTGGTGAG AAGCTAGGCC GTGTAGTACA CATAATTCAG 
TCAAGGGAAC CATCACTTAA AAACTCCAAC CCCGATGAGA TTGAGATTGA CTTTGAGACC 
CTGAAGCCAT CTACACTACG AGAGTTGGAG CGATATGTCA CCTCCTGTTT GCGGAAGAAA 
AGGAAACCTC AAGCTGAAAA AGTTGACGTG ATTGCTGGTT CTTCCAAGAT GAAGGGATTC 
TCATCCTCTG AGTCGGAGAG CACCAGCGAA TCCAGCTCCT CTGACAGTGA AGACTCTGAA 
ACAGAGATGG CTCCCAAGTC AAAAAAGAAG GGGCACACTG GGAGGGACCA GAAAAAGCAT 
CATCACCATC ACCATCCACA GATGCAGCCA GCCCCAGCTC CTGTGCCCCA GCAGCCGCCC 
CCACCTCCAC AGCAGCCTCC TCCACCCCCA CCTCCGCAGC AGCAGCAGCA GCAACCTCCA 
CCCCCACCAC CTCCGCCCTC CATGCCACAG CAGACTGCCC CAGCGATGAA GTCCTCGCCC 
CCACCCTTCA TCACTGCCCA GGTCCCCGTC CTGGAACCAC AGCTGCCAGG CAGTGTCTTT 
GACCCTATTG GCCACTTCAC CCAGCCCATC TTGCACCTGC CGCAGCCGGA GCTGCCTCCT 
CACCTGCCCC AGCCACCTGA GCACAGCACT CCACCCCATC TCAACCAGCA TGCTGTGGTC 
TCTCCTCCAG CTTTGCACAA TGCGCTGCCC CAACAGCCAT CTCGGCCCAG TAACCGAGCT 
GCTGCTCTGC CCCCAAAGCC TACCCGACCC CCAGCTGTGT CCCCTGCCCT GGCCCAGCCC 
CCCCTGCTCC CACAACCACC AATGGCTCAG CCCCCCCAAG TGCTGCTGGA GGATGAAGAG 
CCACCTGCCC CACCCCTCAC CTCCATGCAG ATGCAGTTGT ACCTACAGCA GCTGCAGAAG 
GTGCAGCCTC CCACACCACT ACTCCCTTCC GTGAAGGTGC AGTCCCAACC CCCACCGCCT 
TTGCCGCCTC CACCCCACCC TTCTGTGCAG CAGCAGCAGC TCCAGCCACA GCCACCGCCA 
CCCCCACCTC CTCAGCCACA GCCACCACCC CAGCAACAAC ACCAGCCTCC ACCACGACCA 
GTTCACTTGC CATCCATGCC CTTTTCTGCT CATATTCAGC AGCCCCCACC ACCCCCAGGA 
CAGCAACCTA CTCACCCACC CCCAGGACAG CAGCCCCCAC CACCACAGCC TGCCAAGCCC 
CAGCAAGTCA TCCAGCATCA CCCTTCCCCC CGGCACCACA AGTCAGACCC CTACTCAGCT 
GGTCATCTTC GTGAGGCTCC CTCTCCGCTT ATGATACATT CCCCTCAGAT GCCACAGTTC 
CAGAGCCTGA CCCATCAGTC TCCTCCCCAG CAAAACGTCC AGCCTAAGAA GCAGGTAAAG 
GGCAGGGCTG AGCCACAGCC ACCAGGGCCA GTCATGGGCC AAGGCCAGGG ATGCCCACCT 
GCCTCACCGG CTGCCGTGCC TATGCTGTCC CAGGAGCTGC GACCACCTTC AGTCGTCCAG 
CCCCAGCCCC TGGTGGTAGT AAAGGAGGAG AAGATTCACT CACCAATCAT TCGCAGCGAG 
CCTTTCAGCA CCTCACTTCG ACCAGAGCCC CCCAAGCACC CAGAGAACAT CAAGGCCCCA 
GTCCACCTGC CCCAGCGGCC TGAGATGAAG CCTGTAGACA TAGGGAGGCC TGTGATCCGG 
CCTCCAGAGC AGAGTGCACC CCCACCAGGG GCCCCTGACA AGGACAAACA GAAGCAGGAG 
CCAAAAACAC CAGTGGCACC CAAAAAGGAC CTGAAAATTA AGAACATGGG CTCCTGGGCC 
AGCCTGGTAC AGAAGCATCC GACCACCCCA TCCTCCACAG CCAAGTCCTC AAGTGACAGC 
TTTGAGCATT TCCGCCGTGC TGCTCGGGAG AAGGAGGAGA GGGAAAAGGC CCTGAAGGCT 
CAGGCTGAGC ATGCAGAGAA GGAGAAGGAG CGGCTGAGGC AGGAGCGCAT GAGGAGCCGA 
GAGGATGAAG ATGCGCTGGA ACAGGCACGT CGAGCCCATG AGGAGGCACG ACGGCGCCAG 
GAGCAGCAGC AGCAGCAGCA GCAGCAGAGA CAGGAGCAGC AGCAGCAGCA GCAGCAGGCA 
GCTGCTGTGG CCGCTGCCTC TGCCCCCCAG GCCCAGAGCT CCCAGCCCCA GTCTATGCTG 
GACCAGCAGA GGGAGTTGGC CCGGAAACGA GAGCAGGAGC GGAGGCGCAG GGAGGCAATG 
GCAGCTACAA TTGACATGAA TTTCCAGAGT GATCTTTTGT CAATATTTGA AGAAAATCTT 
TTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524748.1
CDS	275..4480
Translation	MSTESGPGTRLRNLPVMGDGLETSQMSTTQAQAQPQPANAASTNPPPPETSNPNKPKRQTNQLQYLLRVVLKTLWKHQFAWPFQQPVDAVKLNLPDYYKIIKTPMDMGTIKKRLENNYYWNAQECIQDFNTMFTNCYIYNKPGDDIVLMAEALEKLFLQKINELPTEETEIMIVQAKGRGRGRKETGRAAKPGVSTVPNTTQASTSPQTQTPQQNPPPPVQATTHPFPAVTPDLIAQPPVMTMVPPQPLQTPSPVPPQPPPPPAPVPQPVQSHPPIIATTPQPVKTKKGVKRKADTTTPTTIDPIHEPPSLAPEPKTAKLGPRRESSRPVKPPKKDVPDSQQHPGPEKSSKISEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYCDIIKHPMDMSTIKSKLESREYRDAQEFGADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPEEPVVTVSSPAVPPPTKVVAPPSSSDSSSDSSSDSDSSTDDSEEERAQRLAELQEQLKAVHEQLAALSQPQQNKPKKKEKDKKEKKKEKHKKKEEVEENKKSKTKELPPKKTKKNNSSNSNVSKKEPVPTKTKPPPTYESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVDVIAGSSKMKGFSSSESESTSESSSSDSEDSETEMAPKSKKKGHTGRDQKKHHHHHHPQMQPAPAPVPQQPPPPPQQPPPPPPPQQQQQQPPPPPPPPSMPQQTAPAMKSSPPPFITAQVPVLEPQLPGSVFDPIGHFTQPILHLPQPELPPHLPQPPEHSTPPHLNQHAVVSPPALHNALPQQPSRPSNRAAALPPKPTRPPAVSPALAQPPLLPQPPMAQPPQVLLEDEEPPAPPLTSMQMQLYLQQLQKVQPPTPLLPSVKVQSQPPPPLPPPPHPSVQQQQLQPQPPPPPPPQPQPPPQQQHQPPPRPVHLPSMPFSAHIQQPPPPPGQQPTHPPPGQQPPPPQPAKPQQVIQHHPSPRHHKSDPYSAGHLREAPSPLMIHSPQMPQFQSLTHQSPPQQNVQPKKQVKGRAEPQPPGPVMGQGQGCPPASPAAVPMLSQELRPPSVVQPQPLVVVKEEKIHSPIIRSEPFSTSLRPEPPKHPENIKAPVHLPQRPEMKPVDIGRPVIRPPEQSAPPPGAPDKDKQKQEPKTPVAPKKDLKIKNMGSWASLVQKHPTTPSSTAKSSSDSFEHFRRAAREKEEREKALKAQAEHAEKEKERLRQERMRSREDEDALEQARRAHEEARRRQEQQQQQQQQRQEQQQQQQQAAAVAAASAPQAQSSQPQSMLDQQRELARKREQERRRREAMAATIDMNFQSDLLSIFEENLF

Target ORF information:

RefSeq Version	XM_006524685.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus bromodomain containing 4 (Brd4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524685.1

ORF Insert Sequence:

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGCC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGACAGACA 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCCGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAAAAA 
ATCAATGAAC TGCCTACAGA AGAAACTGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGAGGGAGGA AAGAAACAGG TAGGGCAGCA AAGCCTGGTG TATCCACGGT ACCAAACACA 
ACTCAAGCAT CAACTTCTCC GCAGACCCAG ACGCCTCAGC AGAACCCTCC TCCACCTGTG 
CAGGCCACAA CTCACCCCTT TCCTGCTGTC ACCCCAGACC TCATTGCCCA GCCTCCTGTC 
ATGACAATGG TGCCCCCTCA GCCACTTCAG ACTCCTTCAC CGGTACCCCC CCAGCCACCA 
CCCCCACCTG CTCCAGTTCC ACAGCCTGTG CAGAGTCACC CGCCCATCAT TGCGACCACC 
CCCCAGCCTG TGAAGACAAA GAAAGGGGTG AAGAGGAAAG CAGATACCAC CACCCCTACC 
ACCATCGACC CCATTCATGA GCCACCCTCA CTGGCCCCAG AGCCCAAGAC CGCCAAGCTG 
GGTCCTCGGC GGGAGAGCAG CAGACCTGTG AAGCCTCCAA AGAAGGATGT ACCGGACTCA 
CAGCAGCACC CAGGGCCAGA GAAGAGCAGC AAGATCTCTG AGCAGCTAAA GTGCTGCAGT 
GGCATCCTCA AGGAGATGTT TGCCAAGAAA CATGCTGCCT ATGCCTGGCC TTTCTACAAG 
CCTGTGGATG TGGAGGCACT GGGTCTGCAC GACTACTGTG ACATCATCAA ACATCCCATG 
GACATGAGCA CAATCAAGTC TAAACTAGAG TCCCGAGAGT ACAGAGATGC CCAGGAATTT 
GGTGCTGATG TCCGATTGAT GTTCTCCAAC TGCTACAAGT ACAACCCCCC TGACCATGAA 
GTGGTAGCCA TGGCTCGAAA ACTCCAGGAT GTGTTTGAAA TGCGCTTTGC CAAGATGCCT 
GATGAGCCTG AAGAGCCAGT TGTTACAGTG TCCTCTCCTG CAGTGCCACC CCCTACAAAG 
GTGGTAGCCC CACCCTCATC TAGTGACAGC AGCAGCGACA GTTCTTCCGA CAGCGACAGT 
TCCACTGACG ACTCTGAGGA AGAGCGAGCC CAGCGGCTGG CTGAACTCCA GGAACAGCTC 
AAGGCCGTGC ATGAGCAGCT TGCAGCCCTC TCACAGCCCC AGCAGAACAA ACCAAAGAAA 
AAGGAGAAGG ACAAGAAGGA AAAGAAAAAG GAAAAGCACA AAAAGAAAGA AGAAGTGGAG 
GAAAATAAAA AAAGCAAAAC CAAGGAACTT CCTCCCAAAA AGACAAAGAA AAATAACAGC 
AGCAACAGCA ATGTGAGCAA GAAGGAACCA GTACCCACGA AGACCAAGCC GCCTCCCACA 
TATGAATCAG AAGAGGAGGA TAAGTGTAAG CCCATGTCTT ATGAGGAGAA GCGGCAGCTA 
AGTCTAGATA TCAACAAACT TCCTGGTGAG AAGCTAGGCC GTGTAGTACA CATAATTCAG 
TCAAGGGAAC CATCACTTAA AAACTCCAAC CCCGATGAGA TTGAGATTGA CTTTGAGACC 
CTGAAGCCAT CTACACTACG AGAGTTGGAG CGATATGTCA CCTCCTGTTT GCGGAAGAAA 
AGGAAACCTC AAGCTGAAAA AGTTGACGTG ATTGCTGGTT CTTCCAAGAT GAAGGGATTC 
TCATCCTCTG AGTCGGAGAG CACCAGCGAA TCCAGCTCCT CTGACAGTGA AGACTCTGAA 
ACAGAGATGG CTCCCAAGTC AAAAAAGAAG GGGCACACTG GGAGGGACCA GAAAAAGCAT 
CATCACCATC ACCATCCACA GATGCAGCCA GCCCCAGCTC CTGTGCCCCA GCAGCCGCCC 
CCACCTCCAC AGCAGCCTCC TCCACCCCCA CCTCCGCAGC AGCAGCAGCA GCAACCTCCA 
CCCCCACCAC CTCCGCCCTC CATGCCACAG CAGACTGCCC CAGCGATGAA GTCCTCGCCC 
CCACCCTTCA TCACTGCCCA GGTCCCCGTC CTGGAACCAC AGCTGCCAGG CAGTGTCTTT 
GACCCTATTG GCCACTTCAC CCAGCCCATC TTGCACCTGC CGCAGCCGGA GCTGCCTCCT 
CACCTGCCCC AGCCACCTGA GCACAGCACT CCACCCCATC TCAACCAGCA TGCTGTGGTC 
TCTCCTCCAG CTTTGCACAA TGCGCTGCCC CAACAGCCAT CTCGGCCCAG TAACCGAGCT 
GCTGCTCTGC CCCCAAAGCC TACCCGACCC CCAGCTGTGT CCCCTGCCCT GGCCCAGCCC 
CCCCTGCTCC CACAACCACC AATGGCTCAG CCCCCCCAAG TGCTGCTGGA GGATGAAGAG 
CCACCTGCCC CACCCCTCAC CTCCATGCAG ATGCAGTTGT ACCTACAGCA GCTGCAGAAG 
GTGCAGCCTC CCACACCACT ACTCCCTTCC GTGAAGGTGC AGTCCCAACC CCCACCGCCT 
TTGCCGCCTC CACCCCACCC TTCTGTGCAG CAGCAGCAGC TCCAGCCACA GCCACCGCCA 
CCCCCACCTC CTCAGCCACA GCCACCACCC CAGCAACAAC ACCAGCCTCC ACCACGACCA 
GTTCACTTGC CATCCATGCC CTTTTCTGCT CATATTCAGC AGCCCCCACC ACCCCCAGGA 
CAGCAACCTA CTCACCCACC CCCAGGACAG CAGCCCCCAC CACCACAGCC TGCCAAGCCC 
CAGCAAGTCA TCCAGCATCA CCCTTCCCCC CGGCACCACA AGTCAGACCC CTACTCAGCT 
GGTCATCTTC GTGAGGCTCC CTCTCCGCTT ATGATACATT CCCCTCAGAT GCCACAGTTC 
CAGAGCCTGA CCCATCAGTC TCCTCCCCAG CAAAACGTCC AGCCTAAGAA GCAGGTAAAG 
GGCAGGGCTG AGCCACAGCC ACCAGGGCCA GTCATGGGCC AAGGCCAGGG ATGCCCACCT 
GCCTCACCGG CTGCCGTGCC TATGCTGTCC CAGGAGCTGC GACCACCTTC AGTCGTCCAG 
CCCCAGCCCC TGGTGGTAGT AAAGGAGGAG AAGATTCACT CACCAATCAT TCGCAGCGAG 
CCTTTCAGCA CCTCACTTCG ACCAGAGCCC CCCAAGCACC CAGAGAACAT CAAGGCCCCA 
GTCCACCTGC CCCAGCGGCC TGAGATGAAG CCTGTAGACA TAGGGAGGCC TGTGATCCGG 
CCTCCAGAGC AGAGTGCACC CCCACCAGGG GCCCCTGACA AGGACAAACA GAAGCAGGAG 
CCAAAAACAC CAGTGGCACC CAAAAAGGAC CTGAAAATTA AGAACATGGG CTCCTGGGCC 
AGCCTGGTAC AGAAGCATCC GACCACCCCA TCCTCCACAG CCAAGTCCTC AAGTGACAGC 
TTTGAGCATT TCCGCCGTGC TGCTCGGGAG AAGGAGGAGA GGGAAAAGGC CCTGAAGGCT 
CAGGCTGAGC ATGCAGAGAA GGAGAAGGAG CGGCTGAGGC AGGAGCGCAT GAGGAGCCGA 
GAGGATGAAG ATGCGCTGGA ACAGGCACGT CGAGCCCATG AGGAGGCACG ACGGCGCCAG 
GAGCAGCAGC AGCAGCAGCA GCAGCAGAGA CAGGAGCAGC AGCAGCAGCA GCAGCAGGCA 
GCTGCTGTGG CCGCTGCCTC TGCCCCCCAG GCCCAGAGCT CCCAGCCCCA GTCTATGCTG 
GACCAGCAGA GGGAGTTGGC CCGGAAACGA GAGCAGGAGC GGAGGCGCAG GGAGGCAATG 
GCAGCTACAA TTGACATGAA TTTCCAGAGT GATCTTTTGT CAATATTTGA AGAAAATCTT 
TTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brd4 ( XM_006524686.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524749.1 Mus musculus bromodomain containing 4 (Brd4), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMu17143
Clone ID Related Accession (Same CDS sequence)	XM_006524683.2 , XM_006524683.3 , XM_006524684.2 , XM_030249922.1 , XM_006524686.2 , XM_006524687.2 , XM_006524685.2 , XM_030249923.1 , NM_001286630.1 , XM_006524685.1 , XM_006524686.1 , XM_006524684.3
Accession Version	XM_006524686.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4206bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	bromodomain-containing protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGCC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGACAGACA 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCCGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAAAAA 
ATCAATGAAC TGCCTACAGA AGAAACTGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGAGGGAGGA AAGAAACAGG TAGGGCAGCA AAGCCTGGTG TATCCACGGT ACCAAACACA 
ACTCAAGCAT CAACTTCTCC GCAGACCCAG ACGCCTCAGC AGAACCCTCC TCCACCTGTG 
CAGGCCACAA CTCACCCCTT TCCTGCTGTC ACCCCAGACC TCATTGCCCA GCCTCCTGTC 
ATGACAATGG TGCCCCCTCA GCCACTTCAG ACTCCTTCAC CGGTACCCCC CCAGCCACCA 
CCCCCACCTG CTCCAGTTCC ACAGCCTGTG CAGAGTCACC CGCCCATCAT TGCGACCACC 
CCCCAGCCTG TGAAGACAAA GAAAGGGGTG AAGAGGAAAG CAGATACCAC CACCCCTACC 
ACCATCGACC CCATTCATGA GCCACCCTCA CTGGCCCCAG AGCCCAAGAC CGCCAAGCTG 
GGTCCTCGGC GGGAGAGCAG CAGACCTGTG AAGCCTCCAA AGAAGGATGT ACCGGACTCA 
CAGCAGCACC CAGGGCCAGA GAAGAGCAGC AAGATCTCTG AGCAGCTAAA GTGCTGCAGT 
GGCATCCTCA AGGAGATGTT TGCCAAGAAA CATGCTGCCT ATGCCTGGCC TTTCTACAAG 
CCTGTGGATG TGGAGGCACT GGGTCTGCAC GACTACTGTG ACATCATCAA ACATCCCATG 
GACATGAGCA CAATCAAGTC TAAACTAGAG TCCCGAGAGT ACAGAGATGC CCAGGAATTT 
GGTGCTGATG TCCGATTGAT GTTCTCCAAC TGCTACAAGT ACAACCCCCC TGACCATGAA 
GTGGTAGCCA TGGCTCGAAA ACTCCAGGAT GTGTTTGAAA TGCGCTTTGC CAAGATGCCT 
GATGAGCCTG AAGAGCCAGT TGTTACAGTG TCCTCTCCTG CAGTGCCACC CCCTACAAAG 
GTGGTAGCCC CACCCTCATC TAGTGACAGC AGCAGCGACA GTTCTTCCGA CAGCGACAGT 
TCCACTGACG ACTCTGAGGA AGAGCGAGCC CAGCGGCTGG CTGAACTCCA GGAACAGCTC 
AAGGCCGTGC ATGAGCAGCT TGCAGCCCTC TCACAGCCCC AGCAGAACAA ACCAAAGAAA 
AAGGAGAAGG ACAAGAAGGA AAAGAAAAAG GAAAAGCACA AAAAGAAAGA AGAAGTGGAG 
GAAAATAAAA AAAGCAAAAC CAAGGAACTT CCTCCCAAAA AGACAAAGAA AAATAACAGC 
AGCAACAGCA ATGTGAGCAA GAAGGAACCA GTACCCACGA AGACCAAGCC GCCTCCCACA 
TATGAATCAG AAGAGGAGGA TAAGTGTAAG CCCATGTCTT ATGAGGAGAA GCGGCAGCTA 
AGTCTAGATA TCAACAAACT TCCTGGTGAG AAGCTAGGCC GTGTAGTACA CATAATTCAG 
TCAAGGGAAC CATCACTTAA AAACTCCAAC CCCGATGAGA TTGAGATTGA CTTTGAGACC 
CTGAAGCCAT CTACACTACG AGAGTTGGAG CGATATGTCA CCTCCTGTTT GCGGAAGAAA 
AGGAAACCTC AAGCTGAAAA AGTTGACGTG ATTGCTGGTT CTTCCAAGAT GAAGGGATTC 
TCATCCTCTG AGTCGGAGAG CACCAGCGAA TCCAGCTCCT CTGACAGTGA AGACTCTGAA 
ACAGAGATGG CTCCCAAGTC AAAAAAGAAG GGGCACACTG GGAGGGACCA GAAAAAGCAT 
CATCACCATC ACCATCCACA GATGCAGCCA GCCCCAGCTC CTGTGCCCCA GCAGCCGCCC 
CCACCTCCAC AGCAGCCTCC TCCACCCCCA CCTCCGCAGC AGCAGCAGCA GCAACCTCCA 
CCCCCACCAC CTCCGCCCTC CATGCCACAG CAGACTGCCC CAGCGATGAA GTCCTCGCCC 
CCACCCTTCA TCACTGCCCA GGTCCCCGTC CTGGAACCAC AGCTGCCAGG CAGTGTCTTT 
GACCCTATTG GCCACTTCAC CCAGCCCATC TTGCACCTGC CGCAGCCGGA GCTGCCTCCT 
CACCTGCCCC AGCCACCTGA GCACAGCACT CCACCCCATC TCAACCAGCA TGCTGTGGTC 
TCTCCTCCAG CTTTGCACAA TGCGCTGCCC CAACAGCCAT CTCGGCCCAG TAACCGAGCT 
GCTGCTCTGC CCCCAAAGCC TACCCGACCC CCAGCTGTGT CCCCTGCCCT GGCCCAGCCC 
CCCCTGCTCC CACAACCACC AATGGCTCAG CCCCCCCAAG TGCTGCTGGA GGATGAAGAG 
CCACCTGCCC CACCCCTCAC CTCCATGCAG ATGCAGTTGT ACCTACAGCA GCTGCAGAAG 
GTGCAGCCTC CCACACCACT ACTCCCTTCC GTGAAGGTGC AGTCCCAACC CCCACCGCCT 
TTGCCGCCTC CACCCCACCC TTCTGTGCAG CAGCAGCAGC TCCAGCCACA GCCACCGCCA 
CCCCCACCTC CTCAGCCACA GCCACCACCC CAGCAACAAC ACCAGCCTCC ACCACGACCA 
GTTCACTTGC CATCCATGCC CTTTTCTGCT CATATTCAGC AGCCCCCACC ACCCCCAGGA 
CAGCAACCTA CTCACCCACC CCCAGGACAG CAGCCCCCAC CACCACAGCC TGCCAAGCCC 
CAGCAAGTCA TCCAGCATCA CCCTTCCCCC CGGCACCACA AGTCAGACCC CTACTCAGCT 
GGTCATCTTC GTGAGGCTCC CTCTCCGCTT ATGATACATT CCCCTCAGAT GCCACAGTTC 
CAGAGCCTGA CCCATCAGTC TCCTCCCCAG CAAAACGTCC AGCCTAAGAA GCAGGTAAAG 
GGCAGGGCTG AGCCACAGCC ACCAGGGCCA GTCATGGGCC AAGGCCAGGG ATGCCCACCT 
GCCTCACCGG CTGCCGTGCC TATGCTGTCC CAGGAGCTGC GACCACCTTC AGTCGTCCAG 
CCCCAGCCCC TGGTGGTAGT AAAGGAGGAG AAGATTCACT CACCAATCAT TCGCAGCGAG 
CCTTTCAGCA CCTCACTTCG ACCAGAGCCC CCCAAGCACC CAGAGAACAT CAAGGCCCCA 
GTCCACCTGC CCCAGCGGCC TGAGATGAAG CCTGTAGACA TAGGGAGGCC TGTGATCCGG 
CCTCCAGAGC AGAGTGCACC CCCACCAGGG GCCCCTGACA AGGACAAACA GAAGCAGGAG 
CCAAAAACAC CAGTGGCACC CAAAAAGGAC CTGAAAATTA AGAACATGGG CTCCTGGGCC 
AGCCTGGTAC AGAAGCATCC GACCACCCCA TCCTCCACAG CCAAGTCCTC AAGTGACAGC 
TTTGAGCATT TCCGCCGTGC TGCTCGGGAG AAGGAGGAGA GGGAAAAGGC CCTGAAGGCT 
CAGGCTGAGC ATGCAGAGAA GGAGAAGGAG CGGCTGAGGC AGGAGCGCAT GAGGAGCCGA 
GAGGATGAAG ATGCGCTGGA ACAGGCACGT CGAGCCCATG AGGAGGCACG ACGGCGCCAG 
GAGCAGCAGC AGCAGCAGCA GCAGCAGAGA CAGGAGCAGC AGCAGCAGCA GCAGCAGGCA 
GCTGCTGTGG CCGCTGCCTC TGCCCCCCAG GCCCAGAGCT CCCAGCCCCA GTCTATGCTG 
GACCAGCAGA GGGAGTTGGC CCGGAAACGA GAGCAGGAGC GGAGGCGCAG GGAGGCAATG 
GCAGCTACAA TTGACATGAA TTTCCAGAGT GATCTTTTGT CAATATTTGA AGAAAATCTT 
TTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524749.1
CDS	115..4320
Translation	MSTESGPGTRLRNLPVMGDGLETSQMSTTQAQAQPQPANAASTNPPPPETSNPNKPKRQTNQLQYLLRVVLKTLWKHQFAWPFQQPVDAVKLNLPDYYKIIKTPMDMGTIKKRLENNYYWNAQECIQDFNTMFTNCYIYNKPGDDIVLMAEALEKLFLQKINELPTEETEIMIVQAKGRGRGRKETGRAAKPGVSTVPNTTQASTSPQTQTPQQNPPPPVQATTHPFPAVTPDLIAQPPVMTMVPPQPLQTPSPVPPQPPPPPAPVPQPVQSHPPIIATTPQPVKTKKGVKRKADTTTPTTIDPIHEPPSLAPEPKTAKLGPRRESSRPVKPPKKDVPDSQQHPGPEKSSKISEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYCDIIKHPMDMSTIKSKLESREYRDAQEFGADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPEEPVVTVSSPAVPPPTKVVAPPSSSDSSSDSSSDSDSSTDDSEEERAQRLAELQEQLKAVHEQLAALSQPQQNKPKKKEKDKKEKKKEKHKKKEEVEENKKSKTKELPPKKTKKNNSSNSNVSKKEPVPTKTKPPPTYESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVDVIAGSSKMKGFSSSESESTSESSSSDSEDSETEMAPKSKKKGHTGRDQKKHHHHHHPQMQPAPAPVPQQPPPPPQQPPPPPPPQQQQQQPPPPPPPPSMPQQTAPAMKSSPPPFITAQVPVLEPQLPGSVFDPIGHFTQPILHLPQPELPPHLPQPPEHSTPPHLNQHAVVSPPALHNALPQQPSRPSNRAAALPPKPTRPPAVSPALAQPPLLPQPPMAQPPQVLLEDEEPPAPPLTSMQMQLYLQQLQKVQPPTPLLPSVKVQSQPPPPLPPPPHPSVQQQQLQPQPPPPPPPQPQPPPQQQHQPPPRPVHLPSMPFSAHIQQPPPPPGQQPTHPPPGQQPPPPQPAKPQQVIQHHPSPRHHKSDPYSAGHLREAPSPLMIHSPQMPQFQSLTHQSPPQQNVQPKKQVKGRAEPQPPGPVMGQGQGCPPASPAAVPMLSQELRPPSVVQPQPLVVVKEEKIHSPIIRSEPFSTSLRPEPPKHPENIKAPVHLPQRPEMKPVDIGRPVIRPPEQSAPPPGAPDKDKQKQEPKTPVAPKKDLKIKNMGSWASLVQKHPTTPSSTAKSSSDSFEHFRRAAREKEEREKALKAQAEHAEKEKERLRQERMRSREDEDALEQARRAHEEARRRQEQQQQQQQQRQEQQQQQQQAAAVAAASAPQAQSSQPQSMLDQQRELARKREQERRRREAMAATIDMNFQSDLLSIFEENLF

Target ORF information:

RefSeq Version	XM_006524686.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus bromodomain containing 4 (Brd4), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524686.1

ORF Insert Sequence:

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGCC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGACAGACA 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCCGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAAAAA 
ATCAATGAAC TGCCTACAGA AGAAACTGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGAGGGAGGA AAGAAACAGG TAGGGCAGCA AAGCCTGGTG TATCCACGGT ACCAAACACA 
ACTCAAGCAT CAACTTCTCC GCAGACCCAG ACGCCTCAGC AGAACCCTCC TCCACCTGTG 
CAGGCCACAA CTCACCCCTT TCCTGCTGTC ACCCCAGACC TCATTGCCCA GCCTCCTGTC 
ATGACAATGG TGCCCCCTCA GCCACTTCAG ACTCCTTCAC CGGTACCCCC CCAGCCACCA 
CCCCCACCTG CTCCAGTTCC ACAGCCTGTG CAGAGTCACC CGCCCATCAT TGCGACCACC 
CCCCAGCCTG TGAAGACAAA GAAAGGGGTG AAGAGGAAAG CAGATACCAC CACCCCTACC 
ACCATCGACC CCATTCATGA GCCACCCTCA CTGGCCCCAG AGCCCAAGAC CGCCAAGCTG 
GGTCCTCGGC GGGAGAGCAG CAGACCTGTG AAGCCTCCAA AGAAGGATGT ACCGGACTCA 
CAGCAGCACC CAGGGCCAGA GAAGAGCAGC AAGATCTCTG AGCAGCTAAA GTGCTGCAGT 
GGCATCCTCA AGGAGATGTT TGCCAAGAAA CATGCTGCCT ATGCCTGGCC TTTCTACAAG 
CCTGTGGATG TGGAGGCACT GGGTCTGCAC GACTACTGTG ACATCATCAA ACATCCCATG 
GACATGAGCA CAATCAAGTC TAAACTAGAG TCCCGAGAGT ACAGAGATGC CCAGGAATTT 
GGTGCTGATG TCCGATTGAT GTTCTCCAAC TGCTACAAGT ACAACCCCCC TGACCATGAA 
GTGGTAGCCA TGGCTCGAAA ACTCCAGGAT GTGTTTGAAA TGCGCTTTGC CAAGATGCCT 
GATGAGCCTG AAGAGCCAGT TGTTACAGTG TCCTCTCCTG CAGTGCCACC CCCTACAAAG 
GTGGTAGCCC CACCCTCATC TAGTGACAGC AGCAGCGACA GTTCTTCCGA CAGCGACAGT 
TCCACTGACG ACTCTGAGGA AGAGCGAGCC CAGCGGCTGG CTGAACTCCA GGAACAGCTC 
AAGGCCGTGC ATGAGCAGCT TGCAGCCCTC TCACAGCCCC AGCAGAACAA ACCAAAGAAA 
AAGGAGAAGG ACAAGAAGGA AAAGAAAAAG GAAAAGCACA AAAAGAAAGA AGAAGTGGAG 
GAAAATAAAA AAAGCAAAAC CAAGGAACTT CCTCCCAAAA AGACAAAGAA AAATAACAGC 
AGCAACAGCA ATGTGAGCAA GAAGGAACCA GTACCCACGA AGACCAAGCC GCCTCCCACA 
TATGAATCAG AAGAGGAGGA TAAGTGTAAG CCCATGTCTT ATGAGGAGAA GCGGCAGCTA 
AGTCTAGATA TCAACAAACT TCCTGGTGAG AAGCTAGGCC GTGTAGTACA CATAATTCAG 
TCAAGGGAAC CATCACTTAA AAACTCCAAC CCCGATGAGA TTGAGATTGA CTTTGAGACC 
CTGAAGCCAT CTACACTACG AGAGTTGGAG CGATATGTCA CCTCCTGTTT GCGGAAGAAA 
AGGAAACCTC AAGCTGAAAA AGTTGACGTG ATTGCTGGTT CTTCCAAGAT GAAGGGATTC 
TCATCCTCTG AGTCGGAGAG CACCAGCGAA TCCAGCTCCT CTGACAGTGA AGACTCTGAA 
ACAGAGATGG CTCCCAAGTC AAAAAAGAAG GGGCACACTG GGAGGGACCA GAAAAAGCAT 
CATCACCATC ACCATCCACA GATGCAGCCA GCCCCAGCTC CTGTGCCCCA GCAGCCGCCC 
CCACCTCCAC AGCAGCCTCC TCCACCCCCA CCTCCGCAGC AGCAGCAGCA GCAACCTCCA 
CCCCCACCAC CTCCGCCCTC CATGCCACAG CAGACTGCCC CAGCGATGAA GTCCTCGCCC 
CCACCCTTCA TCACTGCCCA GGTCCCCGTC CTGGAACCAC AGCTGCCAGG CAGTGTCTTT 
GACCCTATTG GCCACTTCAC CCAGCCCATC TTGCACCTGC CGCAGCCGGA GCTGCCTCCT 
CACCTGCCCC AGCCACCTGA GCACAGCACT CCACCCCATC TCAACCAGCA TGCTGTGGTC 
TCTCCTCCAG CTTTGCACAA TGCGCTGCCC CAACAGCCAT CTCGGCCCAG TAACCGAGCT 
GCTGCTCTGC CCCCAAAGCC TACCCGACCC CCAGCTGTGT CCCCTGCCCT GGCCCAGCCC 
CCCCTGCTCC CACAACCACC AATGGCTCAG CCCCCCCAAG TGCTGCTGGA GGATGAAGAG 
CCACCTGCCC CACCCCTCAC CTCCATGCAG ATGCAGTTGT ACCTACAGCA GCTGCAGAAG 
GTGCAGCCTC CCACACCACT ACTCCCTTCC GTGAAGGTGC AGTCCCAACC CCCACCGCCT 
TTGCCGCCTC CACCCCACCC TTCTGTGCAG CAGCAGCAGC TCCAGCCACA GCCACCGCCA 
CCCCCACCTC CTCAGCCACA GCCACCACCC CAGCAACAAC ACCAGCCTCC ACCACGACCA 
GTTCACTTGC CATCCATGCC CTTTTCTGCT CATATTCAGC AGCCCCCACC ACCCCCAGGA 
CAGCAACCTA CTCACCCACC CCCAGGACAG CAGCCCCCAC CACCACAGCC TGCCAAGCCC 
CAGCAAGTCA TCCAGCATCA CCCTTCCCCC CGGCACCACA AGTCAGACCC CTACTCAGCT 
GGTCATCTTC GTGAGGCTCC CTCTCCGCTT ATGATACATT CCCCTCAGAT GCCACAGTTC 
CAGAGCCTGA CCCATCAGTC TCCTCCCCAG CAAAACGTCC AGCCTAAGAA GCAGGTAAAG 
GGCAGGGCTG AGCCACAGCC ACCAGGGCCA GTCATGGGCC AAGGCCAGGG ATGCCCACCT 
GCCTCACCGG CTGCCGTGCC TATGCTGTCC CAGGAGCTGC GACCACCTTC AGTCGTCCAG 
CCCCAGCCCC TGGTGGTAGT AAAGGAGGAG AAGATTCACT CACCAATCAT TCGCAGCGAG 
CCTTTCAGCA CCTCACTTCG ACCAGAGCCC CCCAAGCACC CAGAGAACAT CAAGGCCCCA 
GTCCACCTGC CCCAGCGGCC TGAGATGAAG CCTGTAGACA TAGGGAGGCC TGTGATCCGG 
CCTCCAGAGC AGAGTGCACC CCCACCAGGG GCCCCTGACA AGGACAAACA GAAGCAGGAG 
CCAAAAACAC CAGTGGCACC CAAAAAGGAC CTGAAAATTA AGAACATGGG CTCCTGGGCC 
AGCCTGGTAC AGAAGCATCC GACCACCCCA TCCTCCACAG CCAAGTCCTC AAGTGACAGC 
TTTGAGCATT TCCGCCGTGC TGCTCGGGAG AAGGAGGAGA GGGAAAAGGC CCTGAAGGCT 
CAGGCTGAGC ATGCAGAGAA GGAGAAGGAG CGGCTGAGGC AGGAGCGCAT GAGGAGCCGA 
GAGGATGAAG ATGCGCTGGA ACAGGCACGT CGAGCCCATG AGGAGGCACG ACGGCGCCAG 
GAGCAGCAGC AGCAGCAGCA GCAGCAGAGA CAGGAGCAGC AGCAGCAGCA GCAGCAGGCA 
GCTGCTGTGG CCGCTGCCTC TGCCCCCCAG GCCCAGAGCT CCCAGCCCCA GTCTATGCTG 
GACCAGCAGA GGGAGTTGGC CCGGAAACGA GAGCAGGAGC GGAGGCGCAG GGAGGCAATG 
GCAGCTACAA TTGACATGAA TTTCCAGAGT GATCTTTTGT CAATATTTGA AGAAAATCTT 
TTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brd4 ( XM_006524684.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524747.1 Mus musculus bromodomain containing 4 (Brd4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu17143
Clone ID Related Accession (Same CDS sequence)	XM_006524683.2 , XM_006524683.3 , XM_006524684.2 , XM_030249922.1 , XM_006524686.2 , XM_006524687.2 , XM_006524685.2 , XM_030249923.1 , NM_001286630.1 , XM_006524685.1 , XM_006524686.1 , XM_006524684.3
Accession Version	XM_006524684.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4206bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	bromodomain-containing protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006524684.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGCC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGACAGACA 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCCGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAAAAA 
ATCAATGAAC TGCCTACAGA AGAAACTGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGAGGGAGGA AAGAAACAGG TAGGGCAGCA AAGCCTGGTG TATCCACGGT ACCAAACACA 
ACTCAAGCAT CAACTTCTCC GCAGACCCAG ACGCCTCAGC AGAACCCTCC TCCACCTGTG 
CAGGCCACAA CTCACCCCTT TCCTGCTGTC ACCCCAGACC TCATTGCCCA GCCTCCTGTC 
ATGACAATGG TGCCCCCTCA GCCACTTCAG ACTCCTTCAC CGGTACCCCC CCAGCCACCA 
CCCCCACCTG CTCCAGTTCC ACAGCCTGTG CAGAGTCACC CGCCCATCAT TGCGACCACC 
CCCCAGCCTG TGAAGACAAA GAAAGGGGTG AAGAGGAAAG CAGATACCAC CACCCCTACC 
ACCATCGACC CCATTCATGA GCCACCCTCA CTGGCCCCAG AGCCCAAGAC CGCCAAGCTG 
GGTCCTCGGC GGGAGAGCAG CAGACCTGTG AAGCCTCCAA AGAAGGATGT ACCGGACTCA 
CAGCAGCACC CAGGGCCAGA GAAGAGCAGC AAGATCTCTG AGCAGCTAAA GTGCTGCAGT 
GGCATCCTCA AGGAGATGTT TGCCAAGAAA CATGCTGCCT ATGCCTGGCC TTTCTACAAG 
CCTGTGGATG TGGAGGCACT GGGTCTGCAC GACTACTGTG ACATCATCAA ACATCCCATG 
GACATGAGCA CAATCAAGTC TAAACTAGAG TCCCGAGAGT ACAGAGATGC CCAGGAATTT 
GGTGCTGATG TCCGATTGAT GTTCTCCAAC TGCTACAAGT ACAACCCCCC TGACCATGAA 
GTGGTAGCCA TGGCTCGAAA ACTCCAGGAT GTGTTTGAAA TGCGCTTTGC CAAGATGCCT 
GATGAGCCTG AAGAGCCAGT TGTTACAGTG TCCTCTCCTG CAGTGCCACC CCCTACAAAG 
GTGGTAGCCC CACCCTCATC TAGTGACAGC AGCAGCGACA GTTCTTCCGA CAGCGACAGT 
TCCACTGACG ACTCTGAGGA AGAGCGAGCC CAGCGGCTGG CTGAACTCCA GGAACAGCTC 
AAGGCCGTGC ATGAGCAGCT TGCAGCCCTC TCACAGCCCC AGCAGAACAA ACCAAAGAAA 
AAGGAGAAGG ACAAGAAGGA AAAGAAAAAG GAAAAGCACA AAAAGAAAGA AGAAGTGGAG 
GAAAATAAAA AAAGCAAAAC CAAGGAACTT CCTCCCAAAA AGACAAAGAA AAATAACAGC 
AGCAACAGCA ATGTGAGCAA GAAGGAACCA GTACCCACGA AGACCAAGCC GCCTCCCACA 
TATGAATCAG AAGAGGAGGA TAAGTGTAAG CCCATGTCTT ATGAGGAGAA GCGGCAGCTA 
AGTCTAGATA TCAACAAACT TCCTGGTGAG AAGCTAGGCC GTGTAGTACA CATAATTCAG 
TCAAGGGAAC CATCACTTAA AAACTCCAAC CCCGATGAGA TTGAGATTGA CTTTGAGACC 
CTGAAGCCAT CTACACTACG AGAGTTGGAG CGATATGTCA CCTCCTGTTT GCGGAAGAAA 
AGGAAACCTC AAGCTGAAAA AGTTGACGTG ATTGCTGGTT CTTCCAAGAT GAAGGGATTC 
TCATCCTCTG AGTCGGAGAG CACCAGCGAA TCCAGCTCCT CTGACAGTGA AGACTCTGAA 
ACAGAGATGG CTCCCAAGTC AAAAAAGAAG GGGCACACTG GGAGGGACCA GAAAAAGCAT 
CATCACCATC ACCATCCACA GATGCAGCCA GCCCCAGCTC CTGTGCCCCA GCAGCCGCCC 
CCACCTCCAC AGCAGCCTCC TCCACCCCCA CCTCCGCAGC AGCAGCAGCA GCAACCTCCA 
CCCCCACCAC CTCCGCCCTC CATGCCACAG CAGACTGCCC CAGCGATGAA GTCCTCGCCC 
CCACCCTTCA TCACTGCCCA GGTCCCCGTC CTGGAACCAC AGCTGCCAGG CAGTGTCTTT 
GACCCTATTG GCCACTTCAC CCAGCCCATC TTGCACCTGC CGCAGCCGGA GCTGCCTCCT 
CACCTGCCCC AGCCACCTGA GCACAGCACT CCACCCCATC TCAACCAGCA TGCTGTGGTC 
TCTCCTCCAG CTTTGCACAA TGCGCTGCCC CAACAGCCAT CTCGGCCCAG TAACCGAGCT 
GCTGCTCTGC CCCCAAAGCC TACCCGACCC CCAGCTGTGT CCCCTGCCCT GGCCCAGCCC 
CCCCTGCTCC CACAACCACC AATGGCTCAG CCCCCCCAAG TGCTGCTGGA GGATGAAGAG 
CCACCTGCCC CACCCCTCAC CTCCATGCAG ATGCAGTTGT ACCTACAGCA GCTGCAGAAG 
GTGCAGCCTC CCACACCACT ACTCCCTTCC GTGAAGGTGC AGTCCCAACC CCCACCGCCT 
TTGCCGCCTC CACCCCACCC TTCTGTGCAG CAGCAGCAGC TCCAGCCACA GCCACCGCCA 
CCCCCACCTC CTCAGCCACA GCCACCACCC CAGCAACAAC ACCAGCCTCC ACCACGACCA 
GTTCACTTGC CATCCATGCC CTTTTCTGCT CATATTCAGC AGCCCCCACC ACCCCCAGGA 
CAGCAACCTA CTCACCCACC CCCAGGACAG CAGCCCCCAC CACCACAGCC TGCCAAGCCC 
CAGCAAGTCA TCCAGCATCA CCCTTCCCCC CGGCACCACA AGTCAGACCC CTACTCAGCT 
GGTCATCTTC GTGAGGCTCC CTCTCCGCTT ATGATACATT CCCCTCAGAT GCCACAGTTC 
CAGAGCCTGA CCCATCAGTC TCCTCCCCAG CAAAACGTCC AGCCTAAGAA GCAGGTAAAG 
GGCAGGGCTG AGCCACAGCC ACCAGGGCCA GTCATGGGCC AAGGCCAGGG ATGCCCACCT 
GCCTCACCGG CTGCCGTGCC TATGCTGTCC CAGGAGCTGC GACCACCTTC AGTCGTCCAG 
CCCCAGCCCC TGGTGGTAGT AAAGGAGGAG AAGATTCACT CACCAATCAT TCGCAGCGAG 
CCTTTCAGCA CCTCACTTCG ACCAGAGCCC CCCAAGCACC CAGAGAACAT CAAGGCCCCA 
GTCCACCTGC CCCAGCGGCC TGAGATGAAG CCTGTAGACA TAGGGAGGCC TGTGATCCGG 
CCTCCAGAGC AGAGTGCACC CCCACCAGGG GCCCCTGACA AGGACAAACA GAAGCAGGAG 
CCAAAAACAC CAGTGGCACC CAAAAAGGAC CTGAAAATTA AGAACATGGG CTCCTGGGCC 
AGCCTGGTAC AGAAGCATCC GACCACCCCA TCCTCCACAG CCAAGTCCTC AAGTGACAGC 
TTTGAGCATT TCCGCCGTGC TGCTCGGGAG AAGGAGGAGA GGGAAAAGGC CCTGAAGGCT 
CAGGCTGAGC ATGCAGAGAA GGAGAAGGAG CGGCTGAGGC AGGAGCGCAT GAGGAGCCGA 
GAGGATGAAG ATGCGCTGGA ACAGGCACGT CGAGCCCATG AGGAGGCACG ACGGCGCCAG 
GAGCAGCAGC AGCAGCAGCA GCAGCAGAGA CAGGAGCAGC AGCAGCAGCA GCAGCAGGCA 
GCTGCTGTGG CCGCTGCCTC TGCCCCCCAG GCCCAGAGCT CCCAGCCCCA GTCTATGCTG 
GACCAGCAGA GGGAGTTGGC CCGGAAACGA GAGCAGGAGC GGAGGCGCAG GGAGGCAATG 
GCAGCTACAA TTGACATGAA TTTCCAGAGT GATCTTTTGT CAATATTTGA AGAAAATCTT 
TTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524747.1
CDS	349..4554
Translation	MSTESGPGTRLRNLPVMGDGLETSQMSTTQAQAQPQPANAASTNPPPPETSNPNKPKRQTNQLQYLLRVVLKTLWKHQFAWPFQQPVDAVKLNLPDYYKIIKTPMDMGTIKKRLENNYYWNAQECIQDFNTMFTNCYIYNKPGDDIVLMAEALEKLFLQKINELPTEETEIMIVQAKGRGRGRKETGRAAKPGVSTVPNTTQASTSPQTQTPQQNPPPPVQATTHPFPAVTPDLIAQPPVMTMVPPQPLQTPSPVPPQPPPPPAPVPQPVQSHPPIIATTPQPVKTKKGVKRKADTTTPTTIDPIHEPPSLAPEPKTAKLGPRRESSRPVKPPKKDVPDSQQHPGPEKSSKISEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYCDIIKHPMDMSTIKSKLESREYRDAQEFGADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPEEPVVTVSSPAVPPPTKVVAPPSSSDSSSDSSSDSDSSTDDSEEERAQRLAELQEQLKAVHEQLAALSQPQQNKPKKKEKDKKEKKKEKHKKKEEVEENKKSKTKELPPKKTKKNNSSNSNVSKKEPVPTKTKPPPTYESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVDVIAGSSKMKGFSSSESESTSESSSSDSEDSETEMAPKSKKKGHTGRDQKKHHHHHHPQMQPAPAPVPQQPPPPPQQPPPPPPPQQQQQQPPPPPPPPSMPQQTAPAMKSSPPPFITAQVPVLEPQLPGSVFDPIGHFTQPILHLPQPELPPHLPQPPEHSTPPHLNQHAVVSPPALHNALPQQPSRPSNRAAALPPKPTRPPAVSPALAQPPLLPQPPMAQPPQVLLEDEEPPAPPLTSMQMQLYLQQLQKVQPPTPLLPSVKVQSQPPPPLPPPPHPSVQQQQLQPQPPPPPPPQPQPPPQQQHQPPPRPVHLPSMPFSAHIQQPPPPPGQQPTHPPPGQQPPPPQPAKPQQVIQHHPSPRHHKSDPYSAGHLREAPSPLMIHSPQMPQFQSLTHQSPPQQNVQPKKQVKGRAEPQPPGPVMGQGQGCPPASPAAVPMLSQELRPPSVVQPQPLVVVKEEKIHSPIIRSEPFSTSLRPEPPKHPENIKAPVHLPQRPEMKPVDIGRPVIRPPEQSAPPPGAPDKDKQKQEPKTPVAPKKDLKIKNMGSWASLVQKHPTTPSSTAKSSSDSFEHFRRAAREKEEREKALKAQAEHAEKEKERLRQERMRSREDEDALEQARRAHEEARRRQEQQQQQQQQRQEQQQQQQQAAAVAAASAPQAQSSQPQSMLDQQRELARKREQERRRREAMAATIDMNFQSDLLSIFEENLF

Target ORF information:

RefSeq Version	XM_006524684.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus bromodomain containing 4 (Brd4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524684.3

ORF Insert Sequence:

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGCC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGACAGACA 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCCGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAAAAA 
ATCAATGAAC TGCCTACAGA AGAAACTGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGAGGGAGGA AAGAAACAGG TAGGGCAGCA AAGCCTGGTG TATCCACGGT ACCAAACACA 
ACTCAAGCAT CAACTTCTCC GCAGACCCAG ACGCCTCAGC AGAACCCTCC TCCACCTGTG 
CAGGCCACAA CTCACCCCTT TCCTGCTGTC ACCCCAGACC TCATTGCCCA GCCTCCTGTC 
ATGACAATGG TGCCCCCTCA GCCACTTCAG ACTCCTTCAC CGGTACCCCC CCAGCCACCA 
CCCCCACCTG CTCCAGTTCC ACAGCCTGTG CAGAGTCACC CGCCCATCAT TGCGACCACC 
CCCCAGCCTG TGAAGACAAA GAAAGGGGTG AAGAGGAAAG CAGATACCAC CACCCCTACC 
ACCATCGACC CCATTCATGA GCCACCCTCA CTGGCCCCAG AGCCCAAGAC CGCCAAGCTG 
GGTCCTCGGC GGGAGAGCAG CAGACCTGTG AAGCCTCCAA AGAAGGATGT ACCGGACTCA 
CAGCAGCACC CAGGGCCAGA GAAGAGCAGC AAGATCTCTG AGCAGCTAAA GTGCTGCAGT 
GGCATCCTCA AGGAGATGTT TGCCAAGAAA CATGCTGCCT ATGCCTGGCC TTTCTACAAG 
CCTGTGGATG TGGAGGCACT GGGTCTGCAC GACTACTGTG ACATCATCAA ACATCCCATG 
GACATGAGCA CAATCAAGTC TAAACTAGAG TCCCGAGAGT ACAGAGATGC CCAGGAATTT 
GGTGCTGATG TCCGATTGAT GTTCTCCAAC TGCTACAAGT ACAACCCCCC TGACCATGAA 
GTGGTAGCCA TGGCTCGAAA ACTCCAGGAT GTGTTTGAAA TGCGCTTTGC CAAGATGCCT 
GATGAGCCTG AAGAGCCAGT TGTTACAGTG TCCTCTCCTG CAGTGCCACC CCCTACAAAG 
GTGGTAGCCC CACCCTCATC TAGTGACAGC AGCAGCGACA GTTCTTCCGA CAGCGACAGT 
TCCACTGACG ACTCTGAGGA AGAGCGAGCC CAGCGGCTGG CTGAACTCCA GGAACAGCTC 
AAGGCCGTGC ATGAGCAGCT TGCAGCCCTC TCACAGCCCC AGCAGAACAA ACCAAAGAAA 
AAGGAGAAGG ACAAGAAGGA AAAGAAAAAG GAAAAGCACA AAAAGAAAGA AGAAGTGGAG 
GAAAATAAAA AAAGCAAAAC CAAGGAACTT CCTCCCAAAA AGACAAAGAA AAATAACAGC 
AGCAACAGCA ATGTGAGCAA GAAGGAACCA GTACCCACGA AGACCAAGCC GCCTCCCACA 
TATGAATCAG AAGAGGAGGA TAAGTGTAAG CCCATGTCTT ATGAGGAGAA GCGGCAGCTA 
AGTCTAGATA TCAACAAACT TCCTGGTGAG AAGCTAGGCC GTGTAGTACA CATAATTCAG 
TCAAGGGAAC CATCACTTAA AAACTCCAAC CCCGATGAGA TTGAGATTGA CTTTGAGACC 
CTGAAGCCAT CTACACTACG AGAGTTGGAG CGATATGTCA CCTCCTGTTT GCGGAAGAAA 
AGGAAACCTC AAGCTGAAAA AGTTGACGTG ATTGCTGGTT CTTCCAAGAT GAAGGGATTC 
TCATCCTCTG AGTCGGAGAG CACCAGCGAA TCCAGCTCCT CTGACAGTGA AGACTCTGAA 
ACAGAGATGG CTCCCAAGTC AAAAAAGAAG GGGCACACTG GGAGGGACCA GAAAAAGCAT 
CATCACCATC ACCATCCACA GATGCAGCCA GCCCCAGCTC CTGTGCCCCA GCAGCCGCCC 
CCACCTCCAC AGCAGCCTCC TCCACCCCCA CCTCCGCAGC AGCAGCAGCA GCAACCTCCA 
CCCCCACCAC CTCCGCCCTC CATGCCACAG CAGACTGCCC CAGCGATGAA GTCCTCGCCC 
CCACCCTTCA TCACTGCCCA GGTCCCCGTC CTGGAACCAC AGCTGCCAGG CAGTGTCTTT 
GACCCTATTG GCCACTTCAC CCAGCCCATC TTGCACCTGC CGCAGCCGGA GCTGCCTCCT 
CACCTGCCCC AGCCACCTGA GCACAGCACT CCACCCCATC TCAACCAGCA TGCTGTGGTC 
TCTCCTCCAG CTTTGCACAA TGCGCTGCCC CAACAGCCAT CTCGGCCCAG TAACCGAGCT 
GCTGCTCTGC CCCCAAAGCC TACCCGACCC CCAGCTGTGT CCCCTGCCCT GGCCCAGCCC 
CCCCTGCTCC CACAACCACC AATGGCTCAG CCCCCCCAAG TGCTGCTGGA GGATGAAGAG 
CCACCTGCCC CACCCCTCAC CTCCATGCAG ATGCAGTTGT ACCTACAGCA GCTGCAGAAG 
GTGCAGCCTC CCACACCACT ACTCCCTTCC GTGAAGGTGC AGTCCCAACC CCCACCGCCT 
TTGCCGCCTC CACCCCACCC TTCTGTGCAG CAGCAGCAGC TCCAGCCACA GCCACCGCCA 
CCCCCACCTC CTCAGCCACA GCCACCACCC CAGCAACAAC ACCAGCCTCC ACCACGACCA 
GTTCACTTGC CATCCATGCC CTTTTCTGCT CATATTCAGC AGCCCCCACC ACCCCCAGGA 
CAGCAACCTA CTCACCCACC CCCAGGACAG CAGCCCCCAC CACCACAGCC TGCCAAGCCC 
CAGCAAGTCA TCCAGCATCA CCCTTCCCCC CGGCACCACA AGTCAGACCC CTACTCAGCT 
GGTCATCTTC GTGAGGCTCC CTCTCCGCTT ATGATACATT CCCCTCAGAT GCCACAGTTC 
CAGAGCCTGA CCCATCAGTC TCCTCCCCAG CAAAACGTCC AGCCTAAGAA GCAGGTAAAG 
GGCAGGGCTG AGCCACAGCC ACCAGGGCCA GTCATGGGCC AAGGCCAGGG ATGCCCACCT 
GCCTCACCGG CTGCCGTGCC TATGCTGTCC CAGGAGCTGC GACCACCTTC AGTCGTCCAG 
CCCCAGCCCC TGGTGGTAGT AAAGGAGGAG AAGATTCACT CACCAATCAT TCGCAGCGAG 
CCTTTCAGCA CCTCACTTCG ACCAGAGCCC CCCAAGCACC CAGAGAACAT CAAGGCCCCA 
GTCCACCTGC CCCAGCGGCC TGAGATGAAG CCTGTAGACA TAGGGAGGCC TGTGATCCGG 
CCTCCAGAGC AGAGTGCACC CCCACCAGGG GCCCCTGACA AGGACAAACA GAAGCAGGAG 
CCAAAAACAC CAGTGGCACC CAAAAAGGAC CTGAAAATTA AGAACATGGG CTCCTGGGCC 
AGCCTGGTAC AGAAGCATCC GACCACCCCA TCCTCCACAG CCAAGTCCTC AAGTGACAGC 
TTTGAGCATT TCCGCCGTGC TGCTCGGGAG AAGGAGGAGA GGGAAAAGGC CCTGAAGGCT 
CAGGCTGAGC ATGCAGAGAA GGAGAAGGAG CGGCTGAGGC AGGAGCGCAT GAGGAGCCGA 
GAGGATGAAG ATGCGCTGGA ACAGGCACGT CGAGCCCATG AGGAGGCACG ACGGCGCCAG 
GAGCAGCAGC AGCAGCAGCA GCAGCAGAGA CAGGAGCAGC AGCAGCAGCA GCAGCAGGCA 
GCTGCTGTGG CCGCTGCCTC TGCCCCCCAG GCCCAGAGCT CCCAGCCCCA GTCTATGCTG 
GACCAGCAGA GGGAGTTGGC CCGGAAACGA GAGCAGGAGC GGAGGCGCAG GGAGGCAATG 
GCAGCTACAA TTGACATGAA TTTCCAGAGT GATCTTTTGT CAATATTTGA AGAAAATCTT 
TTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brd4 ( XM_006524690.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524753.1 Mus musculus bromodomain containing 4 (Brd4), transcript variant X13, mRNA.

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CloneID	OMu53863
Clone ID Related Accession (Same CDS sequence)	XM_006524690.2 , XM_006524690.3
Accession Version	XM_006524690.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4107bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	bromodomain-containing protein 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006524690.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGCC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGACAGACA 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCCGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAAAAA 
ATCAATGAAC TGCCTACAGA AGAAACTGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGAGGGAGGA AAGAAACAGG TAGGGCAGCA AAGCCTGGTG TATCCACGGT ACCAAACACA 
ACTCAAGCAT CAACTTCTCC GCAGACCCAG ACGCCTCAGC AGAACCCTCC TCCACCTGTG 
CAGGCCACAA CTCACCCCTT TCCTGCTGTC ACCCCAGACC TCATTGCCCA GCCTCCTGTC 
ATGACAATGG TGCCCCCTCA GCCACTTCAG ACTCCTTCAC CGGTACCCCC CCAGCCACCA 
CCCCCACCTG CTCCAGTTCC ACAGCCTGTG CAGAGTCACC CGCCCATCAT TGCGACCACC 
CCCCAGCCTG TGAAGACAAA GAAAGGGGTG AAGAGGAAAG CAGATACCAC CACCCCTACC 
ACCATCGACC CCATTCATGA GCCACCCTCA CTGGCCCCAG AGCCCAAGAC CGCCAAGCTG 
GGTCCTCGGC GGGAGAGCAG CAGACCTGTG AAGCCTCCAA AGAAGGATGT ACCGGACTCA 
CAGCAGCACC CAGGGCCAGA GAAGAGCAGC AAGATCTCTG AGCAGCTAAA GTGCTGCAGT 
GGCATCCTCA AGGAGATGTT TGCCAAGAAA CATGCTGCCT ATGCCTGGCC TTTCTACAAG 
CCTGTGGATG TGGAGGCACT GGGTCTGCAC GACTACTGTG ACATCATCAA ACATCCCATG 
GACATGAGCA CAATCAAGTC TAAACTAGAG TCCCGAGAGT ACAGAGATGC CCAGGAATTT 
GGTGCTGATG TCCGATTGAT GTTCTCCAAC TGCTACAAGT ACAACCCCCC TGACCATGAA 
GTGGTAGCCA TGGCTCGAAA ACTCCAGGAT GTGTTTGAAA TGCGCTTTGC CAAGATGCCT 
GATGAGCCTG AAGAGCCAGT TGTTACAGTG TCCTCTCCTG CAGTGCCACC CCCTACAAAG 
GTGGTAGCCC CACCCTCATC TAGTGACAGC AGCAGCGACA GTTCTTCCGA CAGCGACAGT 
TCCACTGACG ACTCTGAGGA AGAGCGAGCC CAGCGGCTGG CTGAACTCCA GGAACAGCTC 
AAGGCCGTGC ATGAGCAGCT TGCAGCCCTC TCACAGCCCC AGCAGAACAA ACCAAAGAAA 
AAGGAGAAGG ACAAGAAGGA AAAGAAAAAG GAAAAGCACA AAAAGAAAGA AGAAGTGGAG 
GAAAATAAAA AAAGCAAAAC CAAGGAACTT CCTCCCAAAA AGACAAAGAA AAATAACAGC 
AGCAACAGCA ATGTGAGCAA GAAGGAACCA GTACCCACGA AGACCAAGCC GCCTCCCACA 
TATGAATCAG AAGAGGAGGA TAAGTGTAAG CCCATGTCTT ATGAGGAGAA GCGGCAGCTA 
AGTCTAGATA TCAACAAACT TCCTGGTGAG AAGCTAGGCC GTGTAGTACA CATAATTCAG 
TCAAGGGAAC CATCACTTAA AAACTCCAAC CCCGATGAGA TTGAGATTGA CTTTGAGACC 
CTGAAGCCAT CTACACTACG AGAGTTGGAG CGATATGTCA CCTCCTGTTT GCGGAAGAAA 
AGGAAACCTC AAGCTGAAAA AGTTGACGTG ATTGCTGGTT CTTCCAAGAT GAAGGGATTC 
TCATCCTCTG AGTCGGAGAG CACCAGCGAA TCCAGCTCCT CTGACAGTGA AGACTCTGAA 
ACAGAGATGG CTCCCAAGTC AAAAAAGAAG GGGCACACTG GGAGGGACCA GAAAAAGCAT 
CATCACCATC ACCATCCACA GATGCAGCCA GCCCCAGCTC CTGTGCCCCA GCAGCCGCCC 
CCACCTCCAC AGCAGCCTCC TCCACCCCCA CCTCCGCAGC AGCAGCAGCA GCAACCTCCA 
CCCCCACCAC CTCCGCCCTC CATGCCACAG CAGACTGCCC CAGCGATGAA GTCCTCGCCC 
CCACCCTTCA TCACTGCCCA GGTCCCCGTC CTGGAACCAC AGCTGCCAGG CAGTGTCTTT 
GACCCTATTG GCCACTTCAC CCAGCCCATC TTGCACCTGC CGCAGCCGGA GCTGCCTCCT 
CACCTGCCCC AGCCACCTGA GCACAGCACT CCACCCCATC TCAACCAGCA TGCTGTGGTC 
TCTCCTCCAG CTTTGCACAA TGCGCTGCCC CAACAGCCAT CTCGGCCCAG TAACCGAGCT 
GCTGCTCTGC CCCCAAAGCC TACCCGACCC CCAGCTGTGT CCCCTGCCCT GGCCCAGCCC 
CCCCTGCTCC CACAACCACC AATGGCTCAG CCCCCCCAAG TGCTGCTGGA GGATGAAGAG 
CCACCTGCCC CACCCCTCAC CTCCATGCAG ATGCAGTTGT ACCTACAGCA GCTGCAGAAG 
GTGCAGCCTC CCACACCACT ACTCCCTTCC GTGAAGGTGC AGTCCCAACC CCCACCGCCT 
TTGCCGCCTC CACCCCACCC TTCTGTGCAG CAGCAGCAGC TCCAGCCACA GCCACCGCCA 
CCCCCACCTC CTCAGCCACA GCCACCACCC CAGCAACAAC ACCAGCCTCC ACCACGACCA 
GTTCACTTGC CATCCATGCC CTTTTCTGCT CATATTCAGC AGCCCCCACC ACCCCCAGGA 
CAGCAACCTA CTCACCCACC CCCAGGACAG CAGCCCCCAC CACCACAGCC TGCCAAGCCC 
CAGCAAGTCA TCCAGCATCA CCCTTCCCCC CGGCACCACA AGTCAGACCC CTACTCAGCT 
GGTCATCTTC GTGAGGCTCC CTCTCCGCTT ATGATACATT CCCCTCAGAT GCCACAGTTC 
CAGAGCCTGA CCCATCAGTC TCCTCCCCAG CAAAACGTCC AGCCTAAGAA GCAGGAGCTG 
CGACCACCTT CAGTCGTCCA GCCCCAGCCC CTGGTGGTAG TAAAGGAGGA GAAGATTCAC 
TCACCAATCA TTCGCAGCGA GCCTTTCAGC ACCTCACTTC GACCAGAGCC CCCCAAGCAC 
CCAGAGAACA TCAAGGCCCC AGTCCACCTG CCCCAGCGGC CTGAGATGAA GCCTGTAGAC 
ATAGGGAGGC CTGTGATCCG GCCTCCAGAG CAGAGTGCAC CCCCACCAGG GGCCCCTGAC 
AAGGACAAAC AGAAGCAGGA GCCAAAAACA CCAGTGGCAC CCAAAAAGGA CCTGAAAATT 
AAGAACATGG GCTCCTGGGC CAGCCTGGTA CAGAAGCATC CGACCACCCC ATCCTCCACA 
GCCAAGTCCT CAAGTGACAG CTTTGAGCAT TTCCGCCGTG CTGCTCGGGA GAAGGAGGAG 
AGGGAAAAGG CCCTGAAGGC TCAGGCTGAG CATGCAGAGA AGGAGAAGGA GCGGCTGAGG 
CAGGAGCGCA TGAGGAGCCG AGAGGATGAA GATGCGCTGG AACAGGCACG TCGAGCCCAT 
GAGGAGGCAC GACGGCGCCA GGAGCAGCAG CAGCAGCAGC AGCAGCAGAG ACAGGAGCAG 
CAGCAGCAGC AGCAGCAGGC AGCTGCTGTG GCCGCTGCCT CTGCCCCCCA GGCCCAGAGC 
TCCCAGCCCC AGTCTATGCT GGACCAGCAG AGGGAGTTGG CCCGGAAACG AGAGCAGGAG 
CGGAGGCGCA GGGAGGCAAT GGCAGCTACA ATTGACATGA ATTTCCAGAG TGATCTTTTG 
TCAATATTTG AAGAAAATCT TTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524753.1
CDS	261..4367
Translation	MSTESGPGTRLRNLPVMGDGLETSQMSTTQAQAQPQPANAASTNPPPPETSNPNKPKRQTNQLQYLLRVVLKTLWKHQFAWPFQQPVDAVKLNLPDYYKIIKTPMDMGTIKKRLENNYYWNAQECIQDFNTMFTNCYIYNKPGDDIVLMAEALEKLFLQKINELPTEETEIMIVQAKGRGRGRKETGRAAKPGVSTVPNTTQASTSPQTQTPQQNPPPPVQATTHPFPAVTPDLIAQPPVMTMVPPQPLQTPSPVPPQPPPPPAPVPQPVQSHPPIIATTPQPVKTKKGVKRKADTTTPTTIDPIHEPPSLAPEPKTAKLGPRRESSRPVKPPKKDVPDSQQHPGPEKSSKISEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYCDIIKHPMDMSTIKSKLESREYRDAQEFGADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPEEPVVTVSSPAVPPPTKVVAPPSSSDSSSDSSSDSDSSTDDSEEERAQRLAELQEQLKAVHEQLAALSQPQQNKPKKKEKDKKEKKKEKHKKKEEVEENKKSKTKELPPKKTKKNNSSNSNVSKKEPVPTKTKPPPTYESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVDVIAGSSKMKGFSSSESESTSESSSSDSEDSETEMAPKSKKKGHTGRDQKKHHHHHHPQMQPAPAPVPQQPPPPPQQPPPPPPPQQQQQQPPPPPPPPSMPQQTAPAMKSSPPPFITAQVPVLEPQLPGSVFDPIGHFTQPILHLPQPELPPHLPQPPEHSTPPHLNQHAVVSPPALHNALPQQPSRPSNRAAALPPKPTRPPAVSPALAQPPLLPQPPMAQPPQVLLEDEEPPAPPLTSMQMQLYLQQLQKVQPPTPLLPSVKVQSQPPPPLPPPPHPSVQQQQLQPQPPPPPPPQPQPPPQQQHQPPPRPVHLPSMPFSAHIQQPPPPPGQQPTHPPPGQQPPPPQPAKPQQVIQHHPSPRHHKSDPYSAGHLREAPSPLMIHSPQMPQFQSLTHQSPPQQNVQPKKQELRPPSVVQPQPLVVVKEEKIHSPIIRSEPFSTSLRPEPPKHPENIKAPVHLPQRPEMKPVDIGRPVIRPPEQSAPPPGAPDKDKQKQEPKTPVAPKKDLKIKNMGSWASLVQKHPTTPSSTAKSSSDSFEHFRRAAREKEEREKALKAQAEHAEKEKERLRQERMRSREDEDALEQARRAHEEARRRQEQQQQQQQQRQEQQQQQQQAAAVAAASAPQAQSSQPQSMLDQQRELARKREQERRRREAMAATIDMNFQSDLLSIFEENLF

Target ORF information:

RefSeq Version	XM_006524690.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus bromodomain containing 4 (Brd4), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524690.3

ORF Insert Sequence:

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGCC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGACAGACA 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCCGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAAAAA 
ATCAATGAAC TGCCTACAGA AGAAACTGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGAGGGAGGA AAGAAACAGG TAGGGCAGCA AAGCCTGGTG TATCCACGGT ACCAAACACA 
ACTCAAGCAT CAACTTCTCC GCAGACCCAG ACGCCTCAGC AGAACCCTCC TCCACCTGTG 
CAGGCCACAA CTCACCCCTT TCCTGCTGTC ACCCCAGACC TCATTGCCCA GCCTCCTGTC 
ATGACAATGG TGCCCCCTCA GCCACTTCAG ACTCCTTCAC CGGTACCCCC CCAGCCACCA 
CCCCCACCTG CTCCAGTTCC ACAGCCTGTG CAGAGTCACC CGCCCATCAT TGCGACCACC 
CCCCAGCCTG TGAAGACAAA GAAAGGGGTG AAGAGGAAAG CAGATACCAC CACCCCTACC 
ACCATCGACC CCATTCATGA GCCACCCTCA CTGGCCCCAG AGCCCAAGAC CGCCAAGCTG 
GGTCCTCGGC GGGAGAGCAG CAGACCTGTG AAGCCTCCAA AGAAGGATGT ACCGGACTCA 
CAGCAGCACC CAGGGCCAGA GAAGAGCAGC AAGATCTCTG AGCAGCTAAA GTGCTGCAGT 
GGCATCCTCA AGGAGATGTT TGCCAAGAAA CATGCTGCCT ATGCCTGGCC TTTCTACAAG 
CCTGTGGATG TGGAGGCACT GGGTCTGCAC GACTACTGTG ACATCATCAA ACATCCCATG 
GACATGAGCA CAATCAAGTC TAAACTAGAG TCCCGAGAGT ACAGAGATGC CCAGGAATTT 
GGTGCTGATG TCCGATTGAT GTTCTCCAAC TGCTACAAGT ACAACCCCCC TGACCATGAA 
GTGGTAGCCA TGGCTCGAAA ACTCCAGGAT GTGTTTGAAA TGCGCTTTGC CAAGATGCCT 
GATGAGCCTG AAGAGCCAGT TGTTACAGTG TCCTCTCCTG CAGTGCCACC CCCTACAAAG 
GTGGTAGCCC CACCCTCATC TAGTGACAGC AGCAGCGACA GTTCTTCCGA CAGCGACAGT 
TCCACTGACG ACTCTGAGGA AGAGCGAGCC CAGCGGCTGG CTGAACTCCA GGAACAGCTC 
AAGGCCGTGC ATGAGCAGCT TGCAGCCCTC TCACAGCCCC AGCAGAACAA ACCAAAGAAA 
AAGGAGAAGG ACAAGAAGGA AAAGAAAAAG GAAAAGCACA AAAAGAAAGA AGAAGTGGAG 
GAAAATAAAA AAAGCAAAAC CAAGGAACTT CCTCCCAAAA AGACAAAGAA AAATAACAGC 
AGCAACAGCA ATGTGAGCAA GAAGGAACCA GTACCCACGA AGACCAAGCC GCCTCCCACA 
TATGAATCAG AAGAGGAGGA TAAGTGTAAG CCCATGTCTT ATGAGGAGAA GCGGCAGCTA 
AGTCTAGATA TCAACAAACT TCCTGGTGAG AAGCTAGGCC GTGTAGTACA CATAATTCAG 
TCAAGGGAAC CATCACTTAA AAACTCCAAC CCCGATGAGA TTGAGATTGA CTTTGAGACC 
CTGAAGCCAT CTACACTACG AGAGTTGGAG CGATATGTCA CCTCCTGTTT GCGGAAGAAA 
AGGAAACCTC AAGCTGAAAA AGTTGACGTG ATTGCTGGTT CTTCCAAGAT GAAGGGATTC 
TCATCCTCTG AGTCGGAGAG CACCAGCGAA TCCAGCTCCT CTGACAGTGA AGACTCTGAA 
ACAGAGATGG CTCCCAAGTC AAAAAAGAAG GGGCACACTG GGAGGGACCA GAAAAAGCAT 
CATCACCATC ACCATCCACA GATGCAGCCA GCCCCAGCTC CTGTGCCCCA GCAGCCGCCC 
CCACCTCCAC AGCAGCCTCC TCCACCCCCA CCTCCGCAGC AGCAGCAGCA GCAACCTCCA 
CCCCCACCAC CTCCGCCCTC CATGCCACAG CAGACTGCCC CAGCGATGAA GTCCTCGCCC 
CCACCCTTCA TCACTGCCCA GGTCCCCGTC CTGGAACCAC AGCTGCCAGG CAGTGTCTTT 
GACCCTATTG GCCACTTCAC CCAGCCCATC TTGCACCTGC CGCAGCCGGA GCTGCCTCCT 
CACCTGCCCC AGCCACCTGA GCACAGCACT CCACCCCATC TCAACCAGCA TGCTGTGGTC 
TCTCCTCCAG CTTTGCACAA TGCGCTGCCC CAACAGCCAT CTCGGCCCAG TAACCGAGCT 
GCTGCTCTGC CCCCAAAGCC TACCCGACCC CCAGCTGTGT CCCCTGCCCT GGCCCAGCCC 
CCCCTGCTCC CACAACCACC AATGGCTCAG CCCCCCCAAG TGCTGCTGGA GGATGAAGAG 
CCACCTGCCC CACCCCTCAC CTCCATGCAG ATGCAGTTGT ACCTACAGCA GCTGCAGAAG 
GTGCAGCCTC CCACACCACT ACTCCCTTCC GTGAAGGTGC AGTCCCAACC CCCACCGCCT 
TTGCCGCCTC CACCCCACCC TTCTGTGCAG CAGCAGCAGC TCCAGCCACA GCCACCGCCA 
CCCCCACCTC CTCAGCCACA GCCACCACCC CAGCAACAAC ACCAGCCTCC ACCACGACCA 
GTTCACTTGC CATCCATGCC CTTTTCTGCT CATATTCAGC AGCCCCCACC ACCCCCAGGA 
CAGCAACCTA CTCACCCACC CCCAGGACAG CAGCCCCCAC CACCACAGCC TGCCAAGCCC 
CAGCAAGTCA TCCAGCATCA CCCTTCCCCC CGGCACCACA AGTCAGACCC CTACTCAGCT 
GGTCATCTTC GTGAGGCTCC CTCTCCGCTT ATGATACATT CCCCTCAGAT GCCACAGTTC 
CAGAGCCTGA CCCATCAGTC TCCTCCCCAG CAAAACGTCC AGCCTAAGAA GCAGGAGCTG 
CGACCACCTT CAGTCGTCCA GCCCCAGCCC CTGGTGGTAG TAAAGGAGGA GAAGATTCAC 
TCACCAATCA TTCGCAGCGA GCCTTTCAGC ACCTCACTTC GACCAGAGCC CCCCAAGCAC 
CCAGAGAACA TCAAGGCCCC AGTCCACCTG CCCCAGCGGC CTGAGATGAA GCCTGTAGAC 
ATAGGGAGGC CTGTGATCCG GCCTCCAGAG CAGAGTGCAC CCCCACCAGG GGCCCCTGAC 
AAGGACAAAC AGAAGCAGGA GCCAAAAACA CCAGTGGCAC CCAAAAAGGA CCTGAAAATT 
AAGAACATGG GCTCCTGGGC CAGCCTGGTA CAGAAGCATC CGACCACCCC ATCCTCCACA 
GCCAAGTCCT CAAGTGACAG CTTTGAGCAT TTCCGCCGTG CTGCTCGGGA GAAGGAGGAG 
AGGGAAAAGG CCCTGAAGGC TCAGGCTGAG CATGCAGAGA AGGAGAAGGA GCGGCTGAGG 
CAGGAGCGCA TGAGGAGCCG AGAGGATGAA GATGCGCTGG AACAGGCACG TCGAGCCCAT 
GAGGAGGCAC GACGGCGCCA GGAGCAGCAG CAGCAGCAGC AGCAGCAGAG ACAGGAGCAG 
CAGCAGCAGC AGCAGCAGGC AGCTGCTGTG GCCGCTGCCT CTGCCCCCCA GGCCCAGAGC 
TCCCAGCCCC AGTCTATGCT GGACCAGCAG AGGGAGTTGG CCCGGAAACG AGAGCAGGAG 
CGGAGGCGCA GGGAGGCAAT GGCAGCTACA ATTGACATGA ATTTCCAGAG TGATCTTTTG 
TCAATATTTG AAGAAAATCT TTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brd4 ( XM_030249928.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105788.1 Mus musculus bromodomain containing 4 (Brd4), transcript variant X15, mRNA.

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CloneID	OMu53864
Clone ID Related Accession (Same CDS sequence)	XM_030249928.1 , XM_006524691.1 , XM_006524691.2
Accession Version	XM_030249928.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4104bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	bromodomain-containing protein 4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGCC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGACAGACA 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCCGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAAAAA 
ATCAATGAAC TGCCTACAGA AGAAACTGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGAGGGAGGA AAGAAACAGG GGCAGCAAAG CCTGGTGTAT CCACGGTACC AAACACAACT 
CAAGCATCAA CTTCTCCGCA GACCCAGACG CCTCAGCAGA ACCCTCCTCC ACCTGTGCAG 
GCCACAACTC ACCCCTTTCC TGCTGTCACC CCAGACCTCA TTGCCCAGCC TCCTGTCATG 
ACAATGGTGC CCCCTCAGCC ACTTCAGACT CCTTCACCGG TACCCCCCCA GCCACCACCC 
CCACCTGCTC CAGTTCCACA GCCTGTGCAG AGTCACCCGC CCATCATTGC GACCACCCCC 
CAGCCTGTGA AGACAAAGAA AGGGGTGAAG AGGAAAGCAG ATACCACCAC CCCTACCACC 
ATCGACCCCA TTCATGAGCC ACCCTCACTG GCCCCAGAGC CCAAGACCGC CAAGCTGGGT 
CCTCGGCGGG AGAGCAGCAG ACCTGTGAAG CCTCCAAAGA AGGATGTACC GGACTCACAG 
CAGCACCCAG GGCCAGAGAA GAGCAGCAAG ATCTCTGAGC AGCTAAAGTG CTGCAGTGGC 
ATCCTCAAGG AGATGTTTGC CAAGAAACAT GCTGCCTATG CCTGGCCTTT CTACAAGCCT 
GTGGATGTGG AGGCACTGGG TCTGCACGAC TACTGTGACA TCATCAAACA TCCCATGGAC 
ATGAGCACAA TCAAGTCTAA ACTAGAGTCC CGAGAGTACA GAGATGCCCA GGAATTTGGT 
GCTGATGTCC GATTGATGTT CTCCAACTGC TACAAGTACA ACCCCCCTGA CCATGAAGTG 
GTAGCCATGG CTCGAAAACT CCAGGATGTG TTTGAAATGC GCTTTGCCAA GATGCCTGAT 
GAGCCTGAAG AGCCAGTTGT TACAGTGTCC TCTCCTGCAG TGCCACCCCC TACAAAGGTG 
GTAGCCCCAC CCTCATCTAG TGACAGCAGC AGCGACAGTT CTTCCGACAG CGACAGTTCC 
ACTGACGACT CTGAGGAAGA GCGAGCCCAG CGGCTGGCTG AACTCCAGGA ACAGCTCAAG 
GCCGTGCATG AGCAGCTTGC AGCCCTCTCA CAGCCCCAGC AGAACAAACC AAAGAAAAAG 
GAGAAGGACA AGAAGGAAAA GAAAAAGGAA AAGCACAAAA AGAAAGAAGA AGTGGAGGAA 
AATAAAAAAA GCAAAACCAA GGAACTTCCT CCCAAAAAGA CAAAGAAAAA TAACAGCAGC 
AACAGCAATG TGAGCAAGAA GGAACCAGTA CCCACGAAGA CCAAGCCGCC TCCCACATAT 
GAATCAGAAG AGGAGGATAA GTGTAAGCCC ATGTCTTATG AGGAGAAGCG GCAGCTAAGT 
CTAGATATCA ACAAACTTCC TGGTGAGAAG CTAGGCCGTG TAGTACACAT AATTCAGTCA 
AGGGAACCAT CACTTAAAAA CTCCAACCCC GATGAGATTG AGATTGACTT TGAGACCCTG 
AAGCCATCTA CACTACGAGA GTTGGAGCGA TATGTCACCT CCTGTTTGCG GAAGAAAAGG 
AAACCTCAAG CTGAAAAAGT TGACGTGATT GCTGGTTCTT CCAAGATGAA GGGATTCTCA 
TCCTCTGAGT CGGAGAGCAC CAGCGAATCC AGCTCCTCTG ACAGTGAAGA CTCTGAAACA 
GAGATGGCTC CCAAGTCAAA AAAGAAGGGG CACACTGGGA GGGACCAGAA AAAGCATCAT 
CACCATCACC ATCCACAGAT GCAGCCAGCC CCAGCTCCTG TGCCCCAGCA GCCGCCCCCA 
CCTCCACAGC AGCCTCCTCC ACCCCCACCT CCGCAGCAGC AGCAGCAGCA ACCTCCACCC 
CCACCACCTC CGCCCTCCAT GCCACAGCAG ACTGCCCCAG CGATGAAGTC CTCGCCCCCA 
CCCTTCATCA CTGCCCAGGT CCCCGTCCTG GAACCACAGC TGCCAGGCAG TGTCTTTGAC 
CCTATTGGCC ACTTCACCCA GCCCATCTTG CACCTGCCGC AGCCGGAGCT GCCTCCTCAC 
CTGCCCCAGC CACCTGAGCA CAGCACTCCA CCCCATCTCA ACCAGCATGC TGTGGTCTCT 
CCTCCAGCTT TGCACAATGC GCTGCCCCAA CAGCCATCTC GGCCCAGTAA CCGAGCTGCT 
GCTCTGCCCC CAAAGCCTAC CCGACCCCCA GCTGTGTCCC CTGCCCTGGC CCAGCCCCCC 
CTGCTCCCAC AACCACCAAT GGCTCAGCCC CCCCAAGTGC TGCTGGAGGA TGAAGAGCCA 
CCTGCCCCAC CCCTCACCTC CATGCAGATG CAGTTGTACC TACAGCAGCT GCAGAAGGTG 
CAGCCTCCCA CACCACTACT CCCTTCCGTG AAGGTGCAGT CCCAACCCCC ACCGCCTTTG 
CCGCCTCCAC CCCACCCTTC TGTGCAGCAG CAGCAGCTCC AGCCACAGCC ACCGCCACCC 
CCACCTCCTC AGCCACAGCC ACCACCCCAG CAACAACACC AGCCTCCACC ACGACCAGTT 
CACTTGCCAT CCATGCCCTT TTCTGCTCAT ATTCAGCAGC CCCCACCACC CCCAGGACAG 
CAACCTACTC ACCCACCCCC AGGACAGCAG CCCCCACCAC CACAGCCTGC CAAGCCCCAG 
CAAGTCATCC AGCATCACCC TTCCCCCCGG CACCACAAGT CAGACCCCTA CTCAGCTGGT 
CATCTTCGTG AGGCTCCCTC TCCGCTTATG ATACATTCCC CTCAGATGCC ACAGTTCCAG 
AGCCTGACCC ATCAGTCTCC TCCCCAGCAA AACGTCCAGC CTAAGAAGCA GGAGCTGCGA 
CCACCTTCAG TCGTCCAGCC CCAGCCCCTG GTGGTAGTAA AGGAGGAGAA GATTCACTCA 
CCAATCATTC GCAGCGAGCC TTTCAGCACC TCACTTCGAC CAGAGCCCCC CAAGCACCCA 
GAGAACATCA AGGCCCCAGT CCACCTGCCC CAGCGGCCTG AGATGAAGCC TGTAGACATA 
GGGAGGCCTG TGATCCGGCC TCCAGAGCAG AGTGCACCCC CACCAGGGGC CCCTGACAAG 
GACAAACAGA AGCAGGAGCC AAAAACACCA GTGGCACCCA AAAAGGACCT GAAAATTAAG 
AACATGGGCT CCTGGGCCAG CCTGGTACAG AAGCATCCGA CCACCCCATC CTCCACAGCC 
AAGTCCTCAA GTGACAGCTT TGAGCATTTC CGCCGTGCTG CTCGGGAGAA GGAGGAGAGG 
GAAAAGGCCC TGAAGGCTCA GGCTGAGCAT GCAGAGAAGG AGAAGGAGCG GCTGAGGCAG 
GAGCGCATGA GGAGCCGAGA GGATGAAGAT GCGCTGGAAC AGGCACGTCG AGCCCATGAG 
GAGGCACGAC GGCGCCAGGA GCAGCAGCAG CAGCAGCAGC AGCAGAGACA GGAGCAGCAG 
CAGCAGCAGC AGCAGGCAGC TGCTGTGGCC GCTGCCTCTG CCCCCCAGGC CCAGAGCTCC 
CAGCCCCAGT CTATGCTGGA CCAGCAGAGG GAGTTGGCCC GGAAACGAGA GCAGGAGCGG 
AGGCGCAGGG AGGCAATGGC AGCTACAATT GACATGAATT TCCAGAGTGA TCTTTTGTCA 
ATATTTGAAG AAAATCTTTT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105788.1
CDS	478..4581
Translation	MSTESGPGTRLRNLPVMGDGLETSQMSTTQAQAQPQPANAASTNPPPPETSNPNKPKRQTNQLQYLLRVVLKTLWKHQFAWPFQQPVDAVKLNLPDYYKIIKTPMDMGTIKKRLENNYYWNAQECIQDFNTMFTNCYIYNKPGDDIVLMAEALEKLFLQKINELPTEETEIMIVQAKGRGRGRKETGAAKPGVSTVPNTTQASTSPQTQTPQQNPPPPVQATTHPFPAVTPDLIAQPPVMTMVPPQPLQTPSPVPPQPPPPPAPVPQPVQSHPPIIATTPQPVKTKKGVKRKADTTTPTTIDPIHEPPSLAPEPKTAKLGPRRESSRPVKPPKKDVPDSQQHPGPEKSSKISEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYCDIIKHPMDMSTIKSKLESREYRDAQEFGADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPEEPVVTVSSPAVPPPTKVVAPPSSSDSSSDSSSDSDSSTDDSEEERAQRLAELQEQLKAVHEQLAALSQPQQNKPKKKEKDKKEKKKEKHKKKEEVEENKKSKTKELPPKKTKKNNSSNSNVSKKEPVPTKTKPPPTYESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVDVIAGSSKMKGFSSSESESTSESSSSDSEDSETEMAPKSKKKGHTGRDQKKHHHHHHPQMQPAPAPVPQQPPPPPQQPPPPPPPQQQQQQPPPPPPPPSMPQQTAPAMKSSPPPFITAQVPVLEPQLPGSVFDPIGHFTQPILHLPQPELPPHLPQPPEHSTPPHLNQHAVVSPPALHNALPQQPSRPSNRAAALPPKPTRPPAVSPALAQPPLLPQPPMAQPPQVLLEDEEPPAPPLTSMQMQLYLQQLQKVQPPTPLLPSVKVQSQPPPPLPPPPHPSVQQQQLQPQPPPPPPPQPQPPPQQQHQPPPRPVHLPSMPFSAHIQQPPPPPGQQPTHPPPGQQPPPPQPAKPQQVIQHHPSPRHHKSDPYSAGHLREAPSPLMIHSPQMPQFQSLTHQSPPQQNVQPKKQELRPPSVVQPQPLVVVKEEKIHSPIIRSEPFSTSLRPEPPKHPENIKAPVHLPQRPEMKPVDIGRPVIRPPEQSAPPPGAPDKDKQKQEPKTPVAPKKDLKIKNMGSWASLVQKHPTTPSSTAKSSSDSFEHFRRAAREKEEREKALKAQAEHAEKEKERLRQERMRSREDEDALEQARRAHEEARRRQEQQQQQQQQRQEQQQQQQQAAAVAAASAPQAQSSQPQSMLDQQRELARKREQERRRREAMAATIDMNFQSDLLSIFEENLF

Target ORF information:

RefSeq Version	XM_030249928.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus bromodomain containing 4 (Brd4), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249928.1

ORF Insert Sequence:

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGCC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGACAGACA 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCCGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAAAAA 
ATCAATGAAC TGCCTACAGA AGAAACTGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGAGGGAGGA AAGAAACAGG GGCAGCAAAG CCTGGTGTAT CCACGGTACC AAACACAACT 
CAAGCATCAA CTTCTCCGCA GACCCAGACG CCTCAGCAGA ACCCTCCTCC ACCTGTGCAG 
GCCACAACTC ACCCCTTTCC TGCTGTCACC CCAGACCTCA TTGCCCAGCC TCCTGTCATG 
ACAATGGTGC CCCCTCAGCC ACTTCAGACT CCTTCACCGG TACCCCCCCA GCCACCACCC 
CCACCTGCTC CAGTTCCACA GCCTGTGCAG AGTCACCCGC CCATCATTGC GACCACCCCC 
CAGCCTGTGA AGACAAAGAA AGGGGTGAAG AGGAAAGCAG ATACCACCAC CCCTACCACC 
ATCGACCCCA TTCATGAGCC ACCCTCACTG GCCCCAGAGC CCAAGACCGC CAAGCTGGGT 
CCTCGGCGGG AGAGCAGCAG ACCTGTGAAG CCTCCAAAGA AGGATGTACC GGACTCACAG 
CAGCACCCAG GGCCAGAGAA GAGCAGCAAG ATCTCTGAGC AGCTAAAGTG CTGCAGTGGC 
ATCCTCAAGG AGATGTTTGC CAAGAAACAT GCTGCCTATG CCTGGCCTTT CTACAAGCCT 
GTGGATGTGG AGGCACTGGG TCTGCACGAC TACTGTGACA TCATCAAACA TCCCATGGAC 
ATGAGCACAA TCAAGTCTAA ACTAGAGTCC CGAGAGTACA GAGATGCCCA GGAATTTGGT 
GCTGATGTCC GATTGATGTT CTCCAACTGC TACAAGTACA ACCCCCCTGA CCATGAAGTG 
GTAGCCATGG CTCGAAAACT CCAGGATGTG TTTGAAATGC GCTTTGCCAA GATGCCTGAT 
GAGCCTGAAG AGCCAGTTGT TACAGTGTCC TCTCCTGCAG TGCCACCCCC TACAAAGGTG 
GTAGCCCCAC CCTCATCTAG TGACAGCAGC AGCGACAGTT CTTCCGACAG CGACAGTTCC 
ACTGACGACT CTGAGGAAGA GCGAGCCCAG CGGCTGGCTG AACTCCAGGA ACAGCTCAAG 
GCCGTGCATG AGCAGCTTGC AGCCCTCTCA CAGCCCCAGC AGAACAAACC AAAGAAAAAG 
GAGAAGGACA AGAAGGAAAA GAAAAAGGAA AAGCACAAAA AGAAAGAAGA AGTGGAGGAA 
AATAAAAAAA GCAAAACCAA GGAACTTCCT CCCAAAAAGA CAAAGAAAAA TAACAGCAGC 
AACAGCAATG TGAGCAAGAA GGAACCAGTA CCCACGAAGA CCAAGCCGCC TCCCACATAT 
GAATCAGAAG AGGAGGATAA GTGTAAGCCC ATGTCTTATG AGGAGAAGCG GCAGCTAAGT 
CTAGATATCA ACAAACTTCC TGGTGAGAAG CTAGGCCGTG TAGTACACAT AATTCAGTCA 
AGGGAACCAT CACTTAAAAA CTCCAACCCC GATGAGATTG AGATTGACTT TGAGACCCTG 
AAGCCATCTA CACTACGAGA GTTGGAGCGA TATGTCACCT CCTGTTTGCG GAAGAAAAGG 
AAACCTCAAG CTGAAAAAGT TGACGTGATT GCTGGTTCTT CCAAGATGAA GGGATTCTCA 
TCCTCTGAGT CGGAGAGCAC CAGCGAATCC AGCTCCTCTG ACAGTGAAGA CTCTGAAACA 
GAGATGGCTC CCAAGTCAAA AAAGAAGGGG CACACTGGGA GGGACCAGAA AAAGCATCAT 
CACCATCACC ATCCACAGAT GCAGCCAGCC CCAGCTCCTG TGCCCCAGCA GCCGCCCCCA 
CCTCCACAGC AGCCTCCTCC ACCCCCACCT CCGCAGCAGC AGCAGCAGCA ACCTCCACCC 
CCACCACCTC CGCCCTCCAT GCCACAGCAG ACTGCCCCAG CGATGAAGTC CTCGCCCCCA 
CCCTTCATCA CTGCCCAGGT CCCCGTCCTG GAACCACAGC TGCCAGGCAG TGTCTTTGAC 
CCTATTGGCC ACTTCACCCA GCCCATCTTG CACCTGCCGC AGCCGGAGCT GCCTCCTCAC 
CTGCCCCAGC CACCTGAGCA CAGCACTCCA CCCCATCTCA ACCAGCATGC TGTGGTCTCT 
CCTCCAGCTT TGCACAATGC GCTGCCCCAA CAGCCATCTC GGCCCAGTAA CCGAGCTGCT 
GCTCTGCCCC CAAAGCCTAC CCGACCCCCA GCTGTGTCCC CTGCCCTGGC CCAGCCCCCC 
CTGCTCCCAC AACCACCAAT GGCTCAGCCC CCCCAAGTGC TGCTGGAGGA TGAAGAGCCA 
CCTGCCCCAC CCCTCACCTC CATGCAGATG CAGTTGTACC TACAGCAGCT GCAGAAGGTG 
CAGCCTCCCA CACCACTACT CCCTTCCGTG AAGGTGCAGT CCCAACCCCC ACCGCCTTTG 
CCGCCTCCAC CCCACCCTTC TGTGCAGCAG CAGCAGCTCC AGCCACAGCC ACCGCCACCC 
CCACCTCCTC AGCCACAGCC ACCACCCCAG CAACAACACC AGCCTCCACC ACGACCAGTT 
CACTTGCCAT CCATGCCCTT TTCTGCTCAT ATTCAGCAGC CCCCACCACC CCCAGGACAG 
CAACCTACTC ACCCACCCCC AGGACAGCAG CCCCCACCAC CACAGCCTGC CAAGCCCCAG 
CAAGTCATCC AGCATCACCC TTCCCCCCGG CACCACAAGT CAGACCCCTA CTCAGCTGGT 
CATCTTCGTG AGGCTCCCTC TCCGCTTATG ATACATTCCC CTCAGATGCC ACAGTTCCAG 
AGCCTGACCC ATCAGTCTCC TCCCCAGCAA AACGTCCAGC CTAAGAAGCA GGAGCTGCGA 
CCACCTTCAG TCGTCCAGCC CCAGCCCCTG GTGGTAGTAA AGGAGGAGAA GATTCACTCA 
CCAATCATTC GCAGCGAGCC TTTCAGCACC TCACTTCGAC CAGAGCCCCC CAAGCACCCA 
GAGAACATCA AGGCCCCAGT CCACCTGCCC CAGCGGCCTG AGATGAAGCC TGTAGACATA 
GGGAGGCCTG TGATCCGGCC TCCAGAGCAG AGTGCACCCC CACCAGGGGC CCCTGACAAG 
GACAAACAGA AGCAGGAGCC AAAAACACCA GTGGCACCCA AAAAGGACCT GAAAATTAAG 
AACATGGGCT CCTGGGCCAG CCTGGTACAG AAGCATCCGA CCACCCCATC CTCCACAGCC 
AAGTCCTCAA GTGACAGCTT TGAGCATTTC CGCCGTGCTG CTCGGGAGAA GGAGGAGAGG 
GAAAAGGCCC TGAAGGCTCA GGCTGAGCAT GCAGAGAAGG AGAAGGAGCG GCTGAGGCAG 
GAGCGCATGA GGAGCCGAGA GGATGAAGAT GCGCTGGAAC AGGCACGTCG AGCCCATGAG 
GAGGCACGAC GGCGCCAGGA GCAGCAGCAG CAGCAGCAGC AGCAGAGACA GGAGCAGCAG 
CAGCAGCAGC AGCAGGCAGC TGCTGTGGCC GCTGCCTCTG CCCCCCAGGC CCAGAGCTCC 
CAGCCCCAGT CTATGCTGGA CCAGCAGAGG GAGTTGGCCC GGAAACGAGA GCAGGAGCGG 
AGGCGCAGGG AGGCAATGGC AGCTACAATT GACATGAATT TCCAGAGTGA TCTTTTGTCA 
ATATTTGAAG AAAATCTTTT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brd4 ( XM_006524691.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524754.1 Mus musculus bromodomain containing 4 (Brd4), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OMu53864
Clone ID Related Accession (Same CDS sequence)	XM_030249928.1 , XM_006524691.1 , XM_006524691.2
Accession Version	XM_006524691.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4104bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	bromodomain-containing protein 4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006524691.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGCC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGACAGACA 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCCGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAAAAA 
ATCAATGAAC TGCCTACAGA AGAAACTGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGAGGGAGGA AAGAAACAGG GGCAGCAAAG CCTGGTGTAT CCACGGTACC AAACACAACT 
CAAGCATCAA CTTCTCCGCA GACCCAGACG CCTCAGCAGA ACCCTCCTCC ACCTGTGCAG 
GCCACAACTC ACCCCTTTCC TGCTGTCACC CCAGACCTCA TTGCCCAGCC TCCTGTCATG 
ACAATGGTGC CCCCTCAGCC ACTTCAGACT CCTTCACCGG TACCCCCCCA GCCACCACCC 
CCACCTGCTC CAGTTCCACA GCCTGTGCAG AGTCACCCGC CCATCATTGC GACCACCCCC 
CAGCCTGTGA AGACAAAGAA AGGGGTGAAG AGGAAAGCAG ATACCACCAC CCCTACCACC 
ATCGACCCCA TTCATGAGCC ACCCTCACTG GCCCCAGAGC CCAAGACCGC CAAGCTGGGT 
CCTCGGCGGG AGAGCAGCAG ACCTGTGAAG CCTCCAAAGA AGGATGTACC GGACTCACAG 
CAGCACCCAG GGCCAGAGAA GAGCAGCAAG ATCTCTGAGC AGCTAAAGTG CTGCAGTGGC 
ATCCTCAAGG AGATGTTTGC CAAGAAACAT GCTGCCTATG CCTGGCCTTT CTACAAGCCT 
GTGGATGTGG AGGCACTGGG TCTGCACGAC TACTGTGACA TCATCAAACA TCCCATGGAC 
ATGAGCACAA TCAAGTCTAA ACTAGAGTCC CGAGAGTACA GAGATGCCCA GGAATTTGGT 
GCTGATGTCC GATTGATGTT CTCCAACTGC TACAAGTACA ACCCCCCTGA CCATGAAGTG 
GTAGCCATGG CTCGAAAACT CCAGGATGTG TTTGAAATGC GCTTTGCCAA GATGCCTGAT 
GAGCCTGAAG AGCCAGTTGT TACAGTGTCC TCTCCTGCAG TGCCACCCCC TACAAAGGTG 
GTAGCCCCAC CCTCATCTAG TGACAGCAGC AGCGACAGTT CTTCCGACAG CGACAGTTCC 
ACTGACGACT CTGAGGAAGA GCGAGCCCAG CGGCTGGCTG AACTCCAGGA ACAGCTCAAG 
GCCGTGCATG AGCAGCTTGC AGCCCTCTCA CAGCCCCAGC AGAACAAACC AAAGAAAAAG 
GAGAAGGACA AGAAGGAAAA GAAAAAGGAA AAGCACAAAA AGAAAGAAGA AGTGGAGGAA 
AATAAAAAAA GCAAAACCAA GGAACTTCCT CCCAAAAAGA CAAAGAAAAA TAACAGCAGC 
AACAGCAATG TGAGCAAGAA GGAACCAGTA CCCACGAAGA CCAAGCCGCC TCCCACATAT 
GAATCAGAAG AGGAGGATAA GTGTAAGCCC ATGTCTTATG AGGAGAAGCG GCAGCTAAGT 
CTAGATATCA ACAAACTTCC TGGTGAGAAG CTAGGCCGTG TAGTACACAT AATTCAGTCA 
AGGGAACCAT CACTTAAAAA CTCCAACCCC GATGAGATTG AGATTGACTT TGAGACCCTG 
AAGCCATCTA CACTACGAGA GTTGGAGCGA TATGTCACCT CCTGTTTGCG GAAGAAAAGG 
AAACCTCAAG CTGAAAAAGT TGACGTGATT GCTGGTTCTT CCAAGATGAA GGGATTCTCA 
TCCTCTGAGT CGGAGAGCAC CAGCGAATCC AGCTCCTCTG ACAGTGAAGA CTCTGAAACA 
GAGATGGCTC CCAAGTCAAA AAAGAAGGGG CACACTGGGA GGGACCAGAA AAAGCATCAT 
CACCATCACC ATCCACAGAT GCAGCCAGCC CCAGCTCCTG TGCCCCAGCA GCCGCCCCCA 
CCTCCACAGC AGCCTCCTCC ACCCCCACCT CCGCAGCAGC AGCAGCAGCA ACCTCCACCC 
CCACCACCTC CGCCCTCCAT GCCACAGCAG ACTGCCCCAG CGATGAAGTC CTCGCCCCCA 
CCCTTCATCA CTGCCCAGGT CCCCGTCCTG GAACCACAGC TGCCAGGCAG TGTCTTTGAC 
CCTATTGGCC ACTTCACCCA GCCCATCTTG CACCTGCCGC AGCCGGAGCT GCCTCCTCAC 
CTGCCCCAGC CACCTGAGCA CAGCACTCCA CCCCATCTCA ACCAGCATGC TGTGGTCTCT 
CCTCCAGCTT TGCACAATGC GCTGCCCCAA CAGCCATCTC GGCCCAGTAA CCGAGCTGCT 
GCTCTGCCCC CAAAGCCTAC CCGACCCCCA GCTGTGTCCC CTGCCCTGGC CCAGCCCCCC 
CTGCTCCCAC AACCACCAAT GGCTCAGCCC CCCCAAGTGC TGCTGGAGGA TGAAGAGCCA 
CCTGCCCCAC CCCTCACCTC CATGCAGATG CAGTTGTACC TACAGCAGCT GCAGAAGGTG 
CAGCCTCCCA CACCACTACT CCCTTCCGTG AAGGTGCAGT CCCAACCCCC ACCGCCTTTG 
CCGCCTCCAC CCCACCCTTC TGTGCAGCAG CAGCAGCTCC AGCCACAGCC ACCGCCACCC 
CCACCTCCTC AGCCACAGCC ACCACCCCAG CAACAACACC AGCCTCCACC ACGACCAGTT 
CACTTGCCAT CCATGCCCTT TTCTGCTCAT ATTCAGCAGC CCCCACCACC CCCAGGACAG 
CAACCTACTC ACCCACCCCC AGGACAGCAG CCCCCACCAC CACAGCCTGC CAAGCCCCAG 
CAAGTCATCC AGCATCACCC TTCCCCCCGG CACCACAAGT CAGACCCCTA CTCAGCTGGT 
CATCTTCGTG AGGCTCCCTC TCCGCTTATG ATACATTCCC CTCAGATGCC ACAGTTCCAG 
AGCCTGACCC ATCAGTCTCC TCCCCAGCAA AACGTCCAGC CTAAGAAGCA GGAGCTGCGA 
CCACCTTCAG TCGTCCAGCC CCAGCCCCTG GTGGTAGTAA AGGAGGAGAA GATTCACTCA 
CCAATCATTC GCAGCGAGCC TTTCAGCACC TCACTTCGAC CAGAGCCCCC CAAGCACCCA 
GAGAACATCA AGGCCCCAGT CCACCTGCCC CAGCGGCCTG AGATGAAGCC TGTAGACATA 
GGGAGGCCTG TGATCCGGCC TCCAGAGCAG AGTGCACCCC CACCAGGGGC CCCTGACAAG 
GACAAACAGA AGCAGGAGCC AAAAACACCA GTGGCACCCA AAAAGGACCT GAAAATTAAG 
AACATGGGCT CCTGGGCCAG CCTGGTACAG AAGCATCCGA CCACCCCATC CTCCACAGCC 
AAGTCCTCAA GTGACAGCTT TGAGCATTTC CGCCGTGCTG CTCGGGAGAA GGAGGAGAGG 
GAAAAGGCCC TGAAGGCTCA GGCTGAGCAT GCAGAGAAGG AGAAGGAGCG GCTGAGGCAG 
GAGCGCATGA GGAGCCGAGA GGATGAAGAT GCGCTGGAAC AGGCACGTCG AGCCCATGAG 
GAGGCACGAC GGCGCCAGGA GCAGCAGCAG CAGCAGCAGC AGCAGAGACA GGAGCAGCAG 
CAGCAGCAGC AGCAGGCAGC TGCTGTGGCC GCTGCCTCTG CCCCCCAGGC CCAGAGCTCC 
CAGCCCCAGT CTATGCTGGA CCAGCAGAGG GAGTTGGCCC GGAAACGAGA GCAGGAGCGG 
AGGCGCAGGG AGGCAATGGC AGCTACAATT GACATGAATT TCCAGAGTGA TCTTTTGTCA 
ATATTTGAAG AAAATCTTTT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524754.1
CDS	261..4364
Translation	MSTESGPGTRLRNLPVMGDGLETSQMSTTQAQAQPQPANAASTNPPPPETSNPNKPKRQTNQLQYLLRVVLKTLWKHQFAWPFQQPVDAVKLNLPDYYKIIKTPMDMGTIKKRLENNYYWNAQECIQDFNTMFTNCYIYNKPGDDIVLMAEALEKLFLQKINELPTEETEIMIVQAKGRGRGRKETGAAKPGVSTVPNTTQASTSPQTQTPQQNPPPPVQATTHPFPAVTPDLIAQPPVMTMVPPQPLQTPSPVPPQPPPPPAPVPQPVQSHPPIIATTPQPVKTKKGVKRKADTTTPTTIDPIHEPPSLAPEPKTAKLGPRRESSRPVKPPKKDVPDSQQHPGPEKSSKISEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYCDIIKHPMDMSTIKSKLESREYRDAQEFGADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPEEPVVTVSSPAVPPPTKVVAPPSSSDSSSDSSSDSDSSTDDSEEERAQRLAELQEQLKAVHEQLAALSQPQQNKPKKKEKDKKEKKKEKHKKKEEVEENKKSKTKELPPKKTKKNNSSNSNVSKKEPVPTKTKPPPTYESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVDVIAGSSKMKGFSSSESESTSESSSSDSEDSETEMAPKSKKKGHTGRDQKKHHHHHHPQMQPAPAPVPQQPPPPPQQPPPPPPPQQQQQQPPPPPPPPSMPQQTAPAMKSSPPPFITAQVPVLEPQLPGSVFDPIGHFTQPILHLPQPELPPHLPQPPEHSTPPHLNQHAVVSPPALHNALPQQPSRPSNRAAALPPKPTRPPAVSPALAQPPLLPQPPMAQPPQVLLEDEEPPAPPLTSMQMQLYLQQLQKVQPPTPLLPSVKVQSQPPPPLPPPPHPSVQQQQLQPQPPPPPPPQPQPPPQQQHQPPPRPVHLPSMPFSAHIQQPPPPPGQQPTHPPPGQQPPPPQPAKPQQVIQHHPSPRHHKSDPYSAGHLREAPSPLMIHSPQMPQFQSLTHQSPPQQNVQPKKQELRPPSVVQPQPLVVVKEEKIHSPIIRSEPFSTSLRPEPPKHPENIKAPVHLPQRPEMKPVDIGRPVIRPPEQSAPPPGAPDKDKQKQEPKTPVAPKKDLKIKNMGSWASLVQKHPTTPSSTAKSSSDSFEHFRRAAREKEEREKALKAQAEHAEKEKERLRQERMRSREDEDALEQARRAHEEARRRQEQQQQQQQQRQEQQQQQQQAAAVAAASAPQAQSSQPQSMLDQQRELARKREQERRRREAMAATIDMNFQSDLLSIFEENLF

Target ORF information:

RefSeq Version	XM_006524691.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus bromodomain containing 4 (Brd4), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524691.2

ORF Insert Sequence:

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGCC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGACAGACA 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCCGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAAAAA 
ATCAATGAAC TGCCTACAGA AGAAACTGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGAGGGAGGA AAGAAACAGG GGCAGCAAAG CCTGGTGTAT CCACGGTACC AAACACAACT 
CAAGCATCAA CTTCTCCGCA GACCCAGACG CCTCAGCAGA ACCCTCCTCC ACCTGTGCAG 
GCCACAACTC ACCCCTTTCC TGCTGTCACC CCAGACCTCA TTGCCCAGCC TCCTGTCATG 
ACAATGGTGC CCCCTCAGCC ACTTCAGACT CCTTCACCGG TACCCCCCCA GCCACCACCC 
CCACCTGCTC CAGTTCCACA GCCTGTGCAG AGTCACCCGC CCATCATTGC GACCACCCCC 
CAGCCTGTGA AGACAAAGAA AGGGGTGAAG AGGAAAGCAG ATACCACCAC CCCTACCACC 
ATCGACCCCA TTCATGAGCC ACCCTCACTG GCCCCAGAGC CCAAGACCGC CAAGCTGGGT 
CCTCGGCGGG AGAGCAGCAG ACCTGTGAAG CCTCCAAAGA AGGATGTACC GGACTCACAG 
CAGCACCCAG GGCCAGAGAA GAGCAGCAAG ATCTCTGAGC AGCTAAAGTG CTGCAGTGGC 
ATCCTCAAGG AGATGTTTGC CAAGAAACAT GCTGCCTATG CCTGGCCTTT CTACAAGCCT 
GTGGATGTGG AGGCACTGGG TCTGCACGAC TACTGTGACA TCATCAAACA TCCCATGGAC 
ATGAGCACAA TCAAGTCTAA ACTAGAGTCC CGAGAGTACA GAGATGCCCA GGAATTTGGT 
GCTGATGTCC GATTGATGTT CTCCAACTGC TACAAGTACA ACCCCCCTGA CCATGAAGTG 
GTAGCCATGG CTCGAAAACT CCAGGATGTG TTTGAAATGC GCTTTGCCAA GATGCCTGAT 
GAGCCTGAAG AGCCAGTTGT TACAGTGTCC TCTCCTGCAG TGCCACCCCC TACAAAGGTG 
GTAGCCCCAC CCTCATCTAG TGACAGCAGC AGCGACAGTT CTTCCGACAG CGACAGTTCC 
ACTGACGACT CTGAGGAAGA GCGAGCCCAG CGGCTGGCTG AACTCCAGGA ACAGCTCAAG 
GCCGTGCATG AGCAGCTTGC AGCCCTCTCA CAGCCCCAGC AGAACAAACC AAAGAAAAAG 
GAGAAGGACA AGAAGGAAAA GAAAAAGGAA AAGCACAAAA AGAAAGAAGA AGTGGAGGAA 
AATAAAAAAA GCAAAACCAA GGAACTTCCT CCCAAAAAGA CAAAGAAAAA TAACAGCAGC 
AACAGCAATG TGAGCAAGAA GGAACCAGTA CCCACGAAGA CCAAGCCGCC TCCCACATAT 
GAATCAGAAG AGGAGGATAA GTGTAAGCCC ATGTCTTATG AGGAGAAGCG GCAGCTAAGT 
CTAGATATCA ACAAACTTCC TGGTGAGAAG CTAGGCCGTG TAGTACACAT AATTCAGTCA 
AGGGAACCAT CACTTAAAAA CTCCAACCCC GATGAGATTG AGATTGACTT TGAGACCCTG 
AAGCCATCTA CACTACGAGA GTTGGAGCGA TATGTCACCT CCTGTTTGCG GAAGAAAAGG 
AAACCTCAAG CTGAAAAAGT TGACGTGATT GCTGGTTCTT CCAAGATGAA GGGATTCTCA 
TCCTCTGAGT CGGAGAGCAC CAGCGAATCC AGCTCCTCTG ACAGTGAAGA CTCTGAAACA 
GAGATGGCTC CCAAGTCAAA AAAGAAGGGG CACACTGGGA GGGACCAGAA AAAGCATCAT 
CACCATCACC ATCCACAGAT GCAGCCAGCC CCAGCTCCTG TGCCCCAGCA GCCGCCCCCA 
CCTCCACAGC AGCCTCCTCC ACCCCCACCT CCGCAGCAGC AGCAGCAGCA ACCTCCACCC 
CCACCACCTC CGCCCTCCAT GCCACAGCAG ACTGCCCCAG CGATGAAGTC CTCGCCCCCA 
CCCTTCATCA CTGCCCAGGT CCCCGTCCTG GAACCACAGC TGCCAGGCAG TGTCTTTGAC 
CCTATTGGCC ACTTCACCCA GCCCATCTTG CACCTGCCGC AGCCGGAGCT GCCTCCTCAC 
CTGCCCCAGC CACCTGAGCA CAGCACTCCA CCCCATCTCA ACCAGCATGC TGTGGTCTCT 
CCTCCAGCTT TGCACAATGC GCTGCCCCAA CAGCCATCTC GGCCCAGTAA CCGAGCTGCT 
GCTCTGCCCC CAAAGCCTAC CCGACCCCCA GCTGTGTCCC CTGCCCTGGC CCAGCCCCCC 
CTGCTCCCAC AACCACCAAT GGCTCAGCCC CCCCAAGTGC TGCTGGAGGA TGAAGAGCCA 
CCTGCCCCAC CCCTCACCTC CATGCAGATG CAGTTGTACC TACAGCAGCT GCAGAAGGTG 
CAGCCTCCCA CACCACTACT CCCTTCCGTG AAGGTGCAGT CCCAACCCCC ACCGCCTTTG 
CCGCCTCCAC CCCACCCTTC TGTGCAGCAG CAGCAGCTCC AGCCACAGCC ACCGCCACCC 
CCACCTCCTC AGCCACAGCC ACCACCCCAG CAACAACACC AGCCTCCACC ACGACCAGTT 
CACTTGCCAT CCATGCCCTT TTCTGCTCAT ATTCAGCAGC CCCCACCACC CCCAGGACAG 
CAACCTACTC ACCCACCCCC AGGACAGCAG CCCCCACCAC CACAGCCTGC CAAGCCCCAG 
CAAGTCATCC AGCATCACCC TTCCCCCCGG CACCACAAGT CAGACCCCTA CTCAGCTGGT 
CATCTTCGTG AGGCTCCCTC TCCGCTTATG ATACATTCCC CTCAGATGCC ACAGTTCCAG 
AGCCTGACCC ATCAGTCTCC TCCCCAGCAA AACGTCCAGC CTAAGAAGCA GGAGCTGCGA 
CCACCTTCAG TCGTCCAGCC CCAGCCCCTG GTGGTAGTAA AGGAGGAGAA GATTCACTCA 
CCAATCATTC GCAGCGAGCC TTTCAGCACC TCACTTCGAC CAGAGCCCCC CAAGCACCCA 
GAGAACATCA AGGCCCCAGT CCACCTGCCC CAGCGGCCTG AGATGAAGCC TGTAGACATA 
GGGAGGCCTG TGATCCGGCC TCCAGAGCAG AGTGCACCCC CACCAGGGGC CCCTGACAAG 
GACAAACAGA AGCAGGAGCC AAAAACACCA GTGGCACCCA AAAAGGACCT GAAAATTAAG 
AACATGGGCT CCTGGGCCAG CCTGGTACAG AAGCATCCGA CCACCCCATC CTCCACAGCC 
AAGTCCTCAA GTGACAGCTT TGAGCATTTC CGCCGTGCTG CTCGGGAGAA GGAGGAGAGG 
GAAAAGGCCC TGAAGGCTCA GGCTGAGCAT GCAGAGAAGG AGAAGGAGCG GCTGAGGCAG 
GAGCGCATGA GGAGCCGAGA GGATGAAGAT GCGCTGGAAC AGGCACGTCG AGCCCATGAG 
GAGGCACGAC GGCGCCAGGA GCAGCAGCAG CAGCAGCAGC AGCAGAGACA GGAGCAGCAG 
CAGCAGCAGC AGCAGGCAGC TGCTGTGGCC GCTGCCTCTG CCCCCCAGGC CCAGAGCTCC 
CAGCCCCAGT CTATGCTGGA CCAGCAGAGG GAGTTGGCCC GGAAACGAGA GCAGGAGCGG 
AGGCGCAGGG AGGCAATGGC AGCTACAATT GACATGAATT TCCAGAGTGA TCTTTTGTCA 
ATATTTGAAG AAAATCTTTT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brd4 ( XM_030249929.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105789.1 Mus musculus bromodomain containing 4 (Brd4), transcript variant X17, mRNA.

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CloneID	OMu53865
Clone ID Related Accession (Same CDS sequence)	XM_011246543.2 , XM_030249929.1 , XM_011246543.3 , XM_017317588.1 , XM_017317588.2
Accession Version	XM_030249929.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3762bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	bromodomain-containing protein 4 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAAG CTCTGGAGAA GCTCTTCTTG CAAAAAATCA ATGAACTGCC TACAGAAGAA 
ACTGAGATCA TGATAGTCCA GGCAAAAGGA AGAGGACGAG GGAGGAAAGA AACAGGTAGG 
GCAGCAAAGC CTGGTGTATC CACGGTACCA AACACAACTC AAGCATCAAC TTCTCCGCAG 
ACCCAGACGC CTCAGCAGAA CCCTCCTCCA CCTGTGCAGG CCACAACTCA CCCCTTTCCT 
GCTGTCACCC CAGACCTCAT TGCCCAGCCT CCTGTCATGA CAATGGTGCC CCCTCAGCCA 
CTTCAGACTC CTTCACCGGT ACCCCCCCAG CCACCACCCC CACCTGCTCC AGTTCCACAG 
CCTGTGCAGA GTCACCCGCC CATCATTGCG ACCACCCCCC AGCCTGTGAA GACAAAGAAA 
GGGGTGAAGA GGAAAGCAGA TACCACCACC CCTACCACCA TCGACCCCAT TCATGAGCCA 
CCCTCACTGG CCCCAGAGCC CAAGACCGCC AAGCTGGGTC CTCGGCGGGA GAGCAGCAGA 
CCTGTGAAGC CTCCAAAGAA GGATGTACCG GACTCACAGC AGCACCCAGG GCCAGAGAAG 
AGCAGCAAGA TCTCTGAGCA GCTAAAGTGC TGCAGTGGCA TCCTCAAGGA GATGTTTGCC 
AAGAAACATG CTGCCTATGC CTGGCCTTTC TACAAGCCTG TGGATGTGGA GGCACTGGGT 
CTGCACGACT ACTGTGACAT CATCAAACAT CCCATGGACA TGAGCACAAT CAAGTCTAAA 
CTAGAGTCCC GAGAGTACAG AGATGCCCAG GAATTTGGTG CTGATGTCCG ATTGATGTTC 
TCCAACTGCT ACAAGTACAA CCCCCCTGAC CATGAAGTGG TAGCCATGGC TCGAAAACTC 
CAGGATGTGT TTGAAATGCG CTTTGCCAAG ATGCCTGATG AGCCTGAAGA GCCAGTTGTT 
ACAGTGTCCT CTCCTGCAGT GCCACCCCCT ACAAAGGTGG TAGCCCCACC CTCATCTAGT 
GACAGCAGCA GCGACAGTTC TTCCGACAGC GACAGTTCCA CTGACGACTC TGAGGAAGAG 
CGAGCCCAGC GGCTGGCTGA ACTCCAGGAA CAGCTCAAGG CCGTGCATGA GCAGCTTGCA 
GCCCTCTCAC AGCCCCAGCA GAACAAACCA AAGAAAAAGG AGAAGGACAA GAAGGAAAAG 
AAAAAGGAAA AGCACAAAAA GAAAGAAGAA GTGGAGGAAA ATAAAAAAAG CAAAACCAAG 
GAACTTCCTC CCAAAAAGAC AAAGAAAAAT AACAGCAGCA ACAGCAATGT GAGCAAGAAG 
GAACCAGTAC CCACGAAGAC CAAGCCGCCT CCCACATATG AATCAGAAGA GGAGGATAAG 
TGTAAGCCCA TGTCTTATGA GGAGAAGCGG CAGCTAAGTC TAGATATCAA CAAACTTCCT 
GGTGAGAAGC TAGGCCGTGT AGTACACATA ATTCAGTCAA GGGAACCATC ACTTAAAAAC 
TCCAACCCCG ATGAGATTGA GATTGACTTT GAGACCCTGA AGCCATCTAC ACTACGAGAG 
TTGGAGCGAT ATGTCACCTC CTGTTTGCGG AAGAAAAGGA AACCTCAAGC TGAAAAAGTT 
GACGTGATTG CTGGTTCTTC CAAGATGAAG GGATTCTCAT CCTCTGAGTC GGAGAGCACC 
AGCGAATCCA GCTCCTCTGA CAGTGAAGAC TCTGAAACAG AGATGGCTCC CAAGTCAAAA 
AAGAAGGGGC ACACTGGGAG GGACCAGAAA AAGCATCATC ACCATCACCA TCCACAGATG 
CAGCCAGCCC CAGCTCCTGT GCCCCAGCAG CCGCCCCCAC CTCCACAGCA GCCTCCTCCA 
CCCCCACCTC CGCAGCAGCA GCAGCAGCAA CCTCCACCCC CACCACCTCC GCCCTCCATG 
CCACAGCAGA CTGCCCCAGC GATGAAGTCC TCGCCCCCAC CCTTCATCAC TGCCCAGGTC 
CCCGTCCTGG AACCACAGCT GCCAGGCAGT GTCTTTGACC CTATTGGCCA CTTCACCCAG 
CCCATCTTGC ACCTGCCGCA GCCGGAGCTG CCTCCTCACC TGCCCCAGCC ACCTGAGCAC 
AGCACTCCAC CCCATCTCAA CCAGCATGCT GTGGTCTCTC CTCCAGCTTT GCACAATGCG 
CTGCCCCAAC AGCCATCTCG GCCCAGTAAC CGAGCTGCTG CTCTGCCCCC AAAGCCTACC 
CGACCCCCAG CTGTGTCCCC TGCCCTGGCC CAGCCCCCCC TGCTCCCACA ACCACCAATG 
GCTCAGCCCC CCCAAGTGCT GCTGGAGGAT GAAGAGCCAC CTGCCCCACC CCTCACCTCC 
ATGCAGATGC AGTTGTACCT ACAGCAGCTG CAGAAGGTGC AGCCTCCCAC ACCACTACTC 
CCTTCCGTGA AGGTGCAGTC CCAACCCCCA CCGCCTTTGC CGCCTCCACC CCACCCTTCT 
GTGCAGCAGC AGCAGCTCCA GCCACAGCCA CCGCCACCCC CACCTCCTCA GCCACAGCCA 
CCACCCCAGC AACAACACCA GCCTCCACCA CGACCAGTTC ACTTGCCATC CATGCCCTTT 
TCTGCTCATA TTCAGCAGCC CCCACCACCC CCAGGACAGC AACCTACTCA CCCACCCCCA 
GGACAGCAGC CCCCACCACC ACAGCCTGCC AAGCCCCAGC AAGTCATCCA GCATCACCCT 
TCCCCCCGGC ACCACAAGTC AGACCCCTAC TCAGCTGGTC ATCTTCGTGA GGCTCCCTCT 
CCGCTTATGA TACATTCCCC TCAGATGCCA CAGTTCCAGA GCCTGACCCA TCAGTCTCCT 
CCCCAGCAAA ACGTCCAGCC TAAGAAGCAG GTAAAGGGCA GGGCTGAGCC ACAGCCACCA 
GGGCCAGTCA TGGGCCAAGG CCAGGGATGC CCACCTGCCT CACCGGCTGC CGTGCCTATG 
CTGTCCCAGG AGCTGCGACC ACCTTCAGTC GTCCAGCCCC AGCCCCTGGT GGTAGTAAAG 
GAGGAGAAGA TTCACTCACC AATCATTCGC AGCGAGCCTT TCAGCACCTC ACTTCGACCA 
GAGCCCCCCA AGCACCCAGA GAACATCAAG GCCCCAGTCC ACCTGCCCCA GCGGCCTGAG 
ATGAAGCCTG TAGACATAGG GAGGCCTGTG ATCCGGCCTC CAGAGCAGAG TGCACCCCCA 
CCAGGGGCCC CTGACAAGGA CAAACAGAAG CAGGAGCCAA AAACACCAGT GGCACCCAAA 
AAGGACCTGA AAATTAAGAA CATGGGCTCC TGGGCCAGCC TGGTACAGAA GCATCCGACC 
ACCCCATCCT CCACAGCCAA GTCCTCAAGT GACAGCTTTG AGCATTTCCG CCGTGCTGCT 
CGGGAGAAGG AGGAGAGGGA AAAGGCCCTG AAGGCTCAGG CTGAGCATGC AGAGAAGGAG 
AAGGAGCGGC TGAGGCAGGA GCGCATGAGG AGCCGAGAGG ATGAAGATGC GCTGGAACAG 
GCACGTCGAG CCCATGAGGA GGCACGACGG CGCCAGGAGC AGCAGCAGCA GCAGCAGCAG 
CAGAGACAGG AGCAGCAGCA GCAGCAGCAG CAGGCAGCTG CTGTGGCCGC TGCCTCTGCC 
CCCCAGGCCC AGAGCTCCCA GCCCCAGTCT ATGCTGGACC AGCAGAGGGA GTTGGCCCGG 
AAACGAGAGC AGGAGCGGAG GCGCAGGGAG GCAATGGCAG CTACAATTGA CATGAATTTC 
CAGAGTGATC TTTTGTCAAT ATTTGAAGAA AATCTTTTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105789.1
CDS	628..4389
Translation	MAEALEKLFLQKINELPTEETEIMIVQAKGRGRGRKETGRAAKPGVSTVPNTTQASTSPQTQTPQQNPPPPVQATTHPFPAVTPDLIAQPPVMTMVPPQPLQTPSPVPPQPPPPPAPVPQPVQSHPPIIATTPQPVKTKKGVKRKADTTTPTTIDPIHEPPSLAPEPKTAKLGPRRESSRPVKPPKKDVPDSQQHPGPEKSSKISEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYCDIIKHPMDMSTIKSKLESREYRDAQEFGADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPEEPVVTVSSPAVPPPTKVVAPPSSSDSSSDSSSDSDSSTDDSEEERAQRLAELQEQLKAVHEQLAALSQPQQNKPKKKEKDKKEKKKEKHKKKEEVEENKKSKTKELPPKKTKKNNSSNSNVSKKEPVPTKTKPPPTYESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVDVIAGSSKMKGFSSSESESTSESSSSDSEDSETEMAPKSKKKGHTGRDQKKHHHHHHPQMQPAPAPVPQQPPPPPQQPPPPPPPQQQQQQPPPPPPPPSMPQQTAPAMKSSPPPFITAQVPVLEPQLPGSVFDPIGHFTQPILHLPQPELPPHLPQPPEHSTPPHLNQHAVVSPPALHNALPQQPSRPSNRAAALPPKPTRPPAVSPALAQPPLLPQPPMAQPPQVLLEDEEPPAPPLTSMQMQLYLQQLQKVQPPTPLLPSVKVQSQPPPPLPPPPHPSVQQQQLQPQPPPPPPPQPQPPPQQQHQPPPRPVHLPSMPFSAHIQQPPPPPGQQPTHPPPGQQPPPPQPAKPQQVIQHHPSPRHHKSDPYSAGHLREAPSPLMIHSPQMPQFQSLTHQSPPQQNVQPKKQVKGRAEPQPPGPVMGQGQGCPPASPAAVPMLSQELRPPSVVQPQPLVVVKEEKIHSPIIRSEPFSTSLRPEPPKHPENIKAPVHLPQRPEMKPVDIGRPVIRPPEQSAPPPGAPDKDKQKQEPKTPVAPKKDLKIKNMGSWASLVQKHPTTPSSTAKSSSDSFEHFRRAAREKEEREKALKAQAEHAEKEKERLRQERMRSREDEDALEQARRAHEEARRRQEQQQQQQQQRQEQQQQQQQAAAVAAASAPQAQSSQPQSMLDQQRELARKREQERRRREAMAATIDMNFQSDLLSIFEENLF

Target ORF information:

RefSeq Version	XM_030249929.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus bromodomain containing 4 (Brd4), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249929.1

ORF Insert Sequence:

ATGGCAGAAG CTCTGGAGAA GCTCTTCTTG CAAAAAATCA ATGAACTGCC TACAGAAGAA 
ACTGAGATCA TGATAGTCCA GGCAAAAGGA AGAGGACGAG GGAGGAAAGA AACAGGTAGG 
GCAGCAAAGC CTGGTGTATC CACGGTACCA AACACAACTC AAGCATCAAC TTCTCCGCAG 
ACCCAGACGC CTCAGCAGAA CCCTCCTCCA CCTGTGCAGG CCACAACTCA CCCCTTTCCT 
GCTGTCACCC CAGACCTCAT TGCCCAGCCT CCTGTCATGA CAATGGTGCC CCCTCAGCCA 
CTTCAGACTC CTTCACCGGT ACCCCCCCAG CCACCACCCC CACCTGCTCC AGTTCCACAG 
CCTGTGCAGA GTCACCCGCC CATCATTGCG ACCACCCCCC AGCCTGTGAA GACAAAGAAA 
GGGGTGAAGA GGAAAGCAGA TACCACCACC CCTACCACCA TCGACCCCAT TCATGAGCCA 
CCCTCACTGG CCCCAGAGCC CAAGACCGCC AAGCTGGGTC CTCGGCGGGA GAGCAGCAGA 
CCTGTGAAGC CTCCAAAGAA GGATGTACCG GACTCACAGC AGCACCCAGG GCCAGAGAAG 
AGCAGCAAGA TCTCTGAGCA GCTAAAGTGC TGCAGTGGCA TCCTCAAGGA GATGTTTGCC 
AAGAAACATG CTGCCTATGC CTGGCCTTTC TACAAGCCTG TGGATGTGGA GGCACTGGGT 
CTGCACGACT ACTGTGACAT CATCAAACAT CCCATGGACA TGAGCACAAT CAAGTCTAAA 
CTAGAGTCCC GAGAGTACAG AGATGCCCAG GAATTTGGTG CTGATGTCCG ATTGATGTTC 
TCCAACTGCT ACAAGTACAA CCCCCCTGAC CATGAAGTGG TAGCCATGGC TCGAAAACTC 
CAGGATGTGT TTGAAATGCG CTTTGCCAAG ATGCCTGATG AGCCTGAAGA GCCAGTTGTT 
ACAGTGTCCT CTCCTGCAGT GCCACCCCCT ACAAAGGTGG TAGCCCCACC CTCATCTAGT 
GACAGCAGCA GCGACAGTTC TTCCGACAGC GACAGTTCCA CTGACGACTC TGAGGAAGAG 
CGAGCCCAGC GGCTGGCTGA ACTCCAGGAA CAGCTCAAGG CCGTGCATGA GCAGCTTGCA 
GCCCTCTCAC AGCCCCAGCA GAACAAACCA AAGAAAAAGG AGAAGGACAA GAAGGAAAAG 
AAAAAGGAAA AGCACAAAAA GAAAGAAGAA GTGGAGGAAA ATAAAAAAAG CAAAACCAAG 
GAACTTCCTC CCAAAAAGAC AAAGAAAAAT AACAGCAGCA ACAGCAATGT GAGCAAGAAG 
GAACCAGTAC CCACGAAGAC CAAGCCGCCT CCCACATATG AATCAGAAGA GGAGGATAAG 
TGTAAGCCCA TGTCTTATGA GGAGAAGCGG CAGCTAAGTC TAGATATCAA CAAACTTCCT 
GGTGAGAAGC TAGGCCGTGT AGTACACATA ATTCAGTCAA GGGAACCATC ACTTAAAAAC 
TCCAACCCCG ATGAGATTGA GATTGACTTT GAGACCCTGA AGCCATCTAC ACTACGAGAG 
TTGGAGCGAT ATGTCACCTC CTGTTTGCGG AAGAAAAGGA AACCTCAAGC TGAAAAAGTT 
GACGTGATTG CTGGTTCTTC CAAGATGAAG GGATTCTCAT CCTCTGAGTC GGAGAGCACC 
AGCGAATCCA GCTCCTCTGA CAGTGAAGAC TCTGAAACAG AGATGGCTCC CAAGTCAAAA 
AAGAAGGGGC ACACTGGGAG GGACCAGAAA AAGCATCATC ACCATCACCA TCCACAGATG 
CAGCCAGCCC CAGCTCCTGT GCCCCAGCAG CCGCCCCCAC CTCCACAGCA GCCTCCTCCA 
CCCCCACCTC CGCAGCAGCA GCAGCAGCAA CCTCCACCCC CACCACCTCC GCCCTCCATG 
CCACAGCAGA CTGCCCCAGC GATGAAGTCC TCGCCCCCAC CCTTCATCAC TGCCCAGGTC 
CCCGTCCTGG AACCACAGCT GCCAGGCAGT GTCTTTGACC CTATTGGCCA CTTCACCCAG 
CCCATCTTGC ACCTGCCGCA GCCGGAGCTG CCTCCTCACC TGCCCCAGCC ACCTGAGCAC 
AGCACTCCAC CCCATCTCAA CCAGCATGCT GTGGTCTCTC CTCCAGCTTT GCACAATGCG 
CTGCCCCAAC AGCCATCTCG GCCCAGTAAC CGAGCTGCTG CTCTGCCCCC AAAGCCTACC 
CGACCCCCAG CTGTGTCCCC TGCCCTGGCC CAGCCCCCCC TGCTCCCACA ACCACCAATG 
GCTCAGCCCC CCCAAGTGCT GCTGGAGGAT GAAGAGCCAC CTGCCCCACC CCTCACCTCC 
ATGCAGATGC AGTTGTACCT ACAGCAGCTG CAGAAGGTGC AGCCTCCCAC ACCACTACTC 
CCTTCCGTGA AGGTGCAGTC CCAACCCCCA CCGCCTTTGC CGCCTCCACC CCACCCTTCT 
GTGCAGCAGC AGCAGCTCCA GCCACAGCCA CCGCCACCCC CACCTCCTCA GCCACAGCCA 
CCACCCCAGC AACAACACCA GCCTCCACCA CGACCAGTTC ACTTGCCATC CATGCCCTTT 
TCTGCTCATA TTCAGCAGCC CCCACCACCC CCAGGACAGC AACCTACTCA CCCACCCCCA 
GGACAGCAGC CCCCACCACC ACAGCCTGCC AAGCCCCAGC AAGTCATCCA GCATCACCCT 
TCCCCCCGGC ACCACAAGTC AGACCCCTAC TCAGCTGGTC ATCTTCGTGA GGCTCCCTCT 
CCGCTTATGA TACATTCCCC TCAGATGCCA CAGTTCCAGA GCCTGACCCA TCAGTCTCCT 
CCCCAGCAAA ACGTCCAGCC TAAGAAGCAG GTAAAGGGCA GGGCTGAGCC ACAGCCACCA 
GGGCCAGTCA TGGGCCAAGG CCAGGGATGC CCACCTGCCT CACCGGCTGC CGTGCCTATG 
CTGTCCCAGG AGCTGCGACC ACCTTCAGTC GTCCAGCCCC AGCCCCTGGT GGTAGTAAAG 
GAGGAGAAGA TTCACTCACC AATCATTCGC AGCGAGCCTT TCAGCACCTC ACTTCGACCA 
GAGCCCCCCA AGCACCCAGA GAACATCAAG GCCCCAGTCC ACCTGCCCCA GCGGCCTGAG 
ATGAAGCCTG TAGACATAGG GAGGCCTGTG ATCCGGCCTC CAGAGCAGAG TGCACCCCCA 
CCAGGGGCCC CTGACAAGGA CAAACAGAAG CAGGAGCCAA AAACACCAGT GGCACCCAAA 
AAGGACCTGA AAATTAAGAA CATGGGCTCC TGGGCCAGCC TGGTACAGAA GCATCCGACC 
ACCCCATCCT CCACAGCCAA GTCCTCAAGT GACAGCTTTG AGCATTTCCG CCGTGCTGCT 
CGGGAGAAGG AGGAGAGGGA AAAGGCCCTG AAGGCTCAGG CTGAGCATGC AGAGAAGGAG 
AAGGAGCGGC TGAGGCAGGA GCGCATGAGG AGCCGAGAGG ATGAAGATGC GCTGGAACAG 
GCACGTCGAG CCCATGAGGA GGCACGACGG CGCCAGGAGC AGCAGCAGCA GCAGCAGCAG 
CAGAGACAGG AGCAGCAGCA GCAGCAGCAG CAGGCAGCTG CTGTGGCCGC TGCCTCTGCC 
CCCCAGGCCC AGAGCTCCCA GCCCCAGTCT ATGCTGGACC AGCAGAGGGA GTTGGCCCGG 
AAACGAGAGC AGGAGCGGAG GCGCAGGGAG GCAATGGCAG CTACAATTGA CATGAATTTC 
CAGAGTGATC TTTTGTCAAT ATTTGAAGAA AATCTTTTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brd4 ( XM_011246543.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244845.1 Mus musculus bromodomain containing 4 (Brd4), transcript variant X18, mRNA.

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CloneID	OMu53865
Clone ID Related Accession (Same CDS sequence)	XM_011246543.2 , XM_030249929.1 , XM_011246543.3 , XM_017317588.1 , XM_017317588.2
Accession Version	XM_011246543.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3762bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	bromodomain-containing protein 4 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011246543.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAAG CTCTGGAGAA GCTCTTCTTG CAAAAAATCA ATGAACTGCC TACAGAAGAA 
ACTGAGATCA TGATAGTCCA GGCAAAAGGA AGAGGACGAG GGAGGAAAGA AACAGGTAGG 
GCAGCAAAGC CTGGTGTATC CACGGTACCA AACACAACTC AAGCATCAAC TTCTCCGCAG 
ACCCAGACGC CTCAGCAGAA CCCTCCTCCA CCTGTGCAGG CCACAACTCA CCCCTTTCCT 
GCTGTCACCC CAGACCTCAT TGCCCAGCCT CCTGTCATGA CAATGGTGCC CCCTCAGCCA 
CTTCAGACTC CTTCACCGGT ACCCCCCCAG CCACCACCCC CACCTGCTCC AGTTCCACAG 
CCTGTGCAGA GTCACCCGCC CATCATTGCG ACCACCCCCC AGCCTGTGAA GACAAAGAAA 
GGGGTGAAGA GGAAAGCAGA TACCACCACC CCTACCACCA TCGACCCCAT TCATGAGCCA 
CCCTCACTGG CCCCAGAGCC CAAGACCGCC AAGCTGGGTC CTCGGCGGGA GAGCAGCAGA 
CCTGTGAAGC CTCCAAAGAA GGATGTACCG GACTCACAGC AGCACCCAGG GCCAGAGAAG 
AGCAGCAAGA TCTCTGAGCA GCTAAAGTGC TGCAGTGGCA TCCTCAAGGA GATGTTTGCC 
AAGAAACATG CTGCCTATGC CTGGCCTTTC TACAAGCCTG TGGATGTGGA GGCACTGGGT 
CTGCACGACT ACTGTGACAT CATCAAACAT CCCATGGACA TGAGCACAAT CAAGTCTAAA 
CTAGAGTCCC GAGAGTACAG AGATGCCCAG GAATTTGGTG CTGATGTCCG ATTGATGTTC 
TCCAACTGCT ACAAGTACAA CCCCCCTGAC CATGAAGTGG TAGCCATGGC TCGAAAACTC 
CAGGATGTGT TTGAAATGCG CTTTGCCAAG ATGCCTGATG AGCCTGAAGA GCCAGTTGTT 
ACAGTGTCCT CTCCTGCAGT GCCACCCCCT ACAAAGGTGG TAGCCCCACC CTCATCTAGT 
GACAGCAGCA GCGACAGTTC TTCCGACAGC GACAGTTCCA CTGACGACTC TGAGGAAGAG 
CGAGCCCAGC GGCTGGCTGA ACTCCAGGAA CAGCTCAAGG CCGTGCATGA GCAGCTTGCA 
GCCCTCTCAC AGCCCCAGCA GAACAAACCA AAGAAAAAGG AGAAGGACAA GAAGGAAAAG 
AAAAAGGAAA AGCACAAAAA GAAAGAAGAA GTGGAGGAAA ATAAAAAAAG CAAAACCAAG 
GAACTTCCTC CCAAAAAGAC AAAGAAAAAT AACAGCAGCA ACAGCAATGT GAGCAAGAAG 
GAACCAGTAC CCACGAAGAC CAAGCCGCCT CCCACATATG AATCAGAAGA GGAGGATAAG 
TGTAAGCCCA TGTCTTATGA GGAGAAGCGG CAGCTAAGTC TAGATATCAA CAAACTTCCT 
GGTGAGAAGC TAGGCCGTGT AGTACACATA ATTCAGTCAA GGGAACCATC ACTTAAAAAC 
TCCAACCCCG ATGAGATTGA GATTGACTTT GAGACCCTGA AGCCATCTAC ACTACGAGAG 
TTGGAGCGAT ATGTCACCTC CTGTTTGCGG AAGAAAAGGA AACCTCAAGC TGAAAAAGTT 
GACGTGATTG CTGGTTCTTC CAAGATGAAG GGATTCTCAT CCTCTGAGTC GGAGAGCACC 
AGCGAATCCA GCTCCTCTGA CAGTGAAGAC TCTGAAACAG AGATGGCTCC CAAGTCAAAA 
AAGAAGGGGC ACACTGGGAG GGACCAGAAA AAGCATCATC ACCATCACCA TCCACAGATG 
CAGCCAGCCC CAGCTCCTGT GCCCCAGCAG CCGCCCCCAC CTCCACAGCA GCCTCCTCCA 
CCCCCACCTC CGCAGCAGCA GCAGCAGCAA CCTCCACCCC CACCACCTCC GCCCTCCATG 
CCACAGCAGA CTGCCCCAGC GATGAAGTCC TCGCCCCCAC CCTTCATCAC TGCCCAGGTC 
CCCGTCCTGG AACCACAGCT GCCAGGCAGT GTCTTTGACC CTATTGGCCA CTTCACCCAG 
CCCATCTTGC ACCTGCCGCA GCCGGAGCTG CCTCCTCACC TGCCCCAGCC ACCTGAGCAC 
AGCACTCCAC CCCATCTCAA CCAGCATGCT GTGGTCTCTC CTCCAGCTTT GCACAATGCG 
CTGCCCCAAC AGCCATCTCG GCCCAGTAAC CGAGCTGCTG CTCTGCCCCC AAAGCCTACC 
CGACCCCCAG CTGTGTCCCC TGCCCTGGCC CAGCCCCCCC TGCTCCCACA ACCACCAATG 
GCTCAGCCCC CCCAAGTGCT GCTGGAGGAT GAAGAGCCAC CTGCCCCACC CCTCACCTCC 
ATGCAGATGC AGTTGTACCT ACAGCAGCTG CAGAAGGTGC AGCCTCCCAC ACCACTACTC 
CCTTCCGTGA AGGTGCAGTC CCAACCCCCA CCGCCTTTGC CGCCTCCACC CCACCCTTCT 
GTGCAGCAGC AGCAGCTCCA GCCACAGCCA CCGCCACCCC CACCTCCTCA GCCACAGCCA 
CCACCCCAGC AACAACACCA GCCTCCACCA CGACCAGTTC ACTTGCCATC CATGCCCTTT 
TCTGCTCATA TTCAGCAGCC CCCACCACCC CCAGGACAGC AACCTACTCA CCCACCCCCA 
GGACAGCAGC CCCCACCACC ACAGCCTGCC AAGCCCCAGC AAGTCATCCA GCATCACCCT 
TCCCCCCGGC ACCACAAGTC AGACCCCTAC TCAGCTGGTC ATCTTCGTGA GGCTCCCTCT 
CCGCTTATGA TACATTCCCC TCAGATGCCA CAGTTCCAGA GCCTGACCCA TCAGTCTCCT 
CCCCAGCAAA ACGTCCAGCC TAAGAAGCAG GTAAAGGGCA GGGCTGAGCC ACAGCCACCA 
GGGCCAGTCA TGGGCCAAGG CCAGGGATGC CCACCTGCCT CACCGGCTGC CGTGCCTATG 
CTGTCCCAGG AGCTGCGACC ACCTTCAGTC GTCCAGCCCC AGCCCCTGGT GGTAGTAAAG 
GAGGAGAAGA TTCACTCACC AATCATTCGC AGCGAGCCTT TCAGCACCTC ACTTCGACCA 
GAGCCCCCCA AGCACCCAGA GAACATCAAG GCCCCAGTCC ACCTGCCCCA GCGGCCTGAG 
ATGAAGCCTG TAGACATAGG GAGGCCTGTG ATCCGGCCTC CAGAGCAGAG TGCACCCCCA 
CCAGGGGCCC CTGACAAGGA CAAACAGAAG CAGGAGCCAA AAACACCAGT GGCACCCAAA 
AAGGACCTGA AAATTAAGAA CATGGGCTCC TGGGCCAGCC TGGTACAGAA GCATCCGACC 
ACCCCATCCT CCACAGCCAA GTCCTCAAGT GACAGCTTTG AGCATTTCCG CCGTGCTGCT 
CGGGAGAAGG AGGAGAGGGA AAAGGCCCTG AAGGCTCAGG CTGAGCATGC AGAGAAGGAG 
AAGGAGCGGC TGAGGCAGGA GCGCATGAGG AGCCGAGAGG ATGAAGATGC GCTGGAACAG 
GCACGTCGAG CCCATGAGGA GGCACGACGG CGCCAGGAGC AGCAGCAGCA GCAGCAGCAG 
CAGAGACAGG AGCAGCAGCA GCAGCAGCAG CAGGCAGCTG CTGTGGCCGC TGCCTCTGCC 
CCCCAGGCCC AGAGCTCCCA GCCCCAGTCT ATGCTGGACC AGCAGAGGGA GTTGGCCCGG 
AAACGAGAGC AGGAGCGGAG GCGCAGGGAG GCAATGGCAG CTACAATTGA CATGAATTTC 
CAGAGTGATC TTTTGTCAAT ATTTGAAGAA AATCTTTTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244845.1
CDS	223..3984
Translation	MAEALEKLFLQKINELPTEETEIMIVQAKGRGRGRKETGRAAKPGVSTVPNTTQASTSPQTQTPQQNPPPPVQATTHPFPAVTPDLIAQPPVMTMVPPQPLQTPSPVPPQPPPPPAPVPQPVQSHPPIIATTPQPVKTKKGVKRKADTTTPTTIDPIHEPPSLAPEPKTAKLGPRRESSRPVKPPKKDVPDSQQHPGPEKSSKISEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYCDIIKHPMDMSTIKSKLESREYRDAQEFGADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPEEPVVTVSSPAVPPPTKVVAPPSSSDSSSDSSSDSDSSTDDSEEERAQRLAELQEQLKAVHEQLAALSQPQQNKPKKKEKDKKEKKKEKHKKKEEVEENKKSKTKELPPKKTKKNNSSNSNVSKKEPVPTKTKPPPTYESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVDVIAGSSKMKGFSSSESESTSESSSSDSEDSETEMAPKSKKKGHTGRDQKKHHHHHHPQMQPAPAPVPQQPPPPPQQPPPPPPPQQQQQQPPPPPPPPSMPQQTAPAMKSSPPPFITAQVPVLEPQLPGSVFDPIGHFTQPILHLPQPELPPHLPQPPEHSTPPHLNQHAVVSPPALHNALPQQPSRPSNRAAALPPKPTRPPAVSPALAQPPLLPQPPMAQPPQVLLEDEEPPAPPLTSMQMQLYLQQLQKVQPPTPLLPSVKVQSQPPPPLPPPPHPSVQQQQLQPQPPPPPPPQPQPPPQQQHQPPPRPVHLPSMPFSAHIQQPPPPPGQQPTHPPPGQQPPPPQPAKPQQVIQHHPSPRHHKSDPYSAGHLREAPSPLMIHSPQMPQFQSLTHQSPPQQNVQPKKQVKGRAEPQPPGPVMGQGQGCPPASPAAVPMLSQELRPPSVVQPQPLVVVKEEKIHSPIIRSEPFSTSLRPEPPKHPENIKAPVHLPQRPEMKPVDIGRPVIRPPEQSAPPPGAPDKDKQKQEPKTPVAPKKDLKIKNMGSWASLVQKHPTTPSSTAKSSSDSFEHFRRAAREKEEREKALKAQAEHAEKEKERLRQERMRSREDEDALEQARRAHEEARRRQEQQQQQQQQRQEQQQQQQQAAAVAAASAPQAQSSQPQSMLDQQRELARKREQERRRREAMAATIDMNFQSDLLSIFEENLF

Target ORF information:

RefSeq Version	XM_011246543.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus bromodomain containing 4 (Brd4), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011246543.3

ORF Insert Sequence:

ATGGCAGAAG CTCTGGAGAA GCTCTTCTTG CAAAAAATCA ATGAACTGCC TACAGAAGAA 
ACTGAGATCA TGATAGTCCA GGCAAAAGGA AGAGGACGAG GGAGGAAAGA AACAGGTAGG 
GCAGCAAAGC CTGGTGTATC CACGGTACCA AACACAACTC AAGCATCAAC TTCTCCGCAG 
ACCCAGACGC CTCAGCAGAA CCCTCCTCCA CCTGTGCAGG CCACAACTCA CCCCTTTCCT 
GCTGTCACCC CAGACCTCAT TGCCCAGCCT CCTGTCATGA CAATGGTGCC CCCTCAGCCA 
CTTCAGACTC CTTCACCGGT ACCCCCCCAG CCACCACCCC CACCTGCTCC AGTTCCACAG 
CCTGTGCAGA GTCACCCGCC CATCATTGCG ACCACCCCCC AGCCTGTGAA GACAAAGAAA 
GGGGTGAAGA GGAAAGCAGA TACCACCACC CCTACCACCA TCGACCCCAT TCATGAGCCA 
CCCTCACTGG CCCCAGAGCC CAAGACCGCC AAGCTGGGTC CTCGGCGGGA GAGCAGCAGA 
CCTGTGAAGC CTCCAAAGAA GGATGTACCG GACTCACAGC AGCACCCAGG GCCAGAGAAG 
AGCAGCAAGA TCTCTGAGCA GCTAAAGTGC TGCAGTGGCA TCCTCAAGGA GATGTTTGCC 
AAGAAACATG CTGCCTATGC CTGGCCTTTC TACAAGCCTG TGGATGTGGA GGCACTGGGT 
CTGCACGACT ACTGTGACAT CATCAAACAT CCCATGGACA TGAGCACAAT CAAGTCTAAA 
CTAGAGTCCC GAGAGTACAG AGATGCCCAG GAATTTGGTG CTGATGTCCG ATTGATGTTC 
TCCAACTGCT ACAAGTACAA CCCCCCTGAC CATGAAGTGG TAGCCATGGC TCGAAAACTC 
CAGGATGTGT TTGAAATGCG CTTTGCCAAG ATGCCTGATG AGCCTGAAGA GCCAGTTGTT 
ACAGTGTCCT CTCCTGCAGT GCCACCCCCT ACAAAGGTGG TAGCCCCACC CTCATCTAGT 
GACAGCAGCA GCGACAGTTC TTCCGACAGC GACAGTTCCA CTGACGACTC TGAGGAAGAG 
CGAGCCCAGC GGCTGGCTGA ACTCCAGGAA CAGCTCAAGG CCGTGCATGA GCAGCTTGCA 
GCCCTCTCAC AGCCCCAGCA GAACAAACCA AAGAAAAAGG AGAAGGACAA GAAGGAAAAG 
AAAAAGGAAA AGCACAAAAA GAAAGAAGAA GTGGAGGAAA ATAAAAAAAG CAAAACCAAG 
GAACTTCCTC CCAAAAAGAC AAAGAAAAAT AACAGCAGCA ACAGCAATGT GAGCAAGAAG 
GAACCAGTAC CCACGAAGAC CAAGCCGCCT CCCACATATG AATCAGAAGA GGAGGATAAG 
TGTAAGCCCA TGTCTTATGA GGAGAAGCGG CAGCTAAGTC TAGATATCAA CAAACTTCCT 
GGTGAGAAGC TAGGCCGTGT AGTACACATA ATTCAGTCAA GGGAACCATC ACTTAAAAAC 
TCCAACCCCG ATGAGATTGA GATTGACTTT GAGACCCTGA AGCCATCTAC ACTACGAGAG 
TTGGAGCGAT ATGTCACCTC CTGTTTGCGG AAGAAAAGGA AACCTCAAGC TGAAAAAGTT 
GACGTGATTG CTGGTTCTTC CAAGATGAAG GGATTCTCAT CCTCTGAGTC GGAGAGCACC 
AGCGAATCCA GCTCCTCTGA CAGTGAAGAC TCTGAAACAG AGATGGCTCC CAAGTCAAAA 
AAGAAGGGGC ACACTGGGAG GGACCAGAAA AAGCATCATC ACCATCACCA TCCACAGATG 
CAGCCAGCCC CAGCTCCTGT GCCCCAGCAG CCGCCCCCAC CTCCACAGCA GCCTCCTCCA 
CCCCCACCTC CGCAGCAGCA GCAGCAGCAA CCTCCACCCC CACCACCTCC GCCCTCCATG 
CCACAGCAGA CTGCCCCAGC GATGAAGTCC TCGCCCCCAC CCTTCATCAC TGCCCAGGTC 
CCCGTCCTGG AACCACAGCT GCCAGGCAGT GTCTTTGACC CTATTGGCCA CTTCACCCAG 
CCCATCTTGC ACCTGCCGCA GCCGGAGCTG CCTCCTCACC TGCCCCAGCC ACCTGAGCAC 
AGCACTCCAC CCCATCTCAA CCAGCATGCT GTGGTCTCTC CTCCAGCTTT GCACAATGCG 
CTGCCCCAAC AGCCATCTCG GCCCAGTAAC CGAGCTGCTG CTCTGCCCCC AAAGCCTACC 
CGACCCCCAG CTGTGTCCCC TGCCCTGGCC CAGCCCCCCC TGCTCCCACA ACCACCAATG 
GCTCAGCCCC CCCAAGTGCT GCTGGAGGAT GAAGAGCCAC CTGCCCCACC CCTCACCTCC 
ATGCAGATGC AGTTGTACCT ACAGCAGCTG CAGAAGGTGC AGCCTCCCAC ACCACTACTC 
CCTTCCGTGA AGGTGCAGTC CCAACCCCCA CCGCCTTTGC CGCCTCCACC CCACCCTTCT 
GTGCAGCAGC AGCAGCTCCA GCCACAGCCA CCGCCACCCC CACCTCCTCA GCCACAGCCA 
CCACCCCAGC AACAACACCA GCCTCCACCA CGACCAGTTC ACTTGCCATC CATGCCCTTT 
TCTGCTCATA TTCAGCAGCC CCCACCACCC CCAGGACAGC AACCTACTCA CCCACCCCCA 
GGACAGCAGC CCCCACCACC ACAGCCTGCC AAGCCCCAGC AAGTCATCCA GCATCACCCT 
TCCCCCCGGC ACCACAAGTC AGACCCCTAC TCAGCTGGTC ATCTTCGTGA GGCTCCCTCT 
CCGCTTATGA TACATTCCCC TCAGATGCCA CAGTTCCAGA GCCTGACCCA TCAGTCTCCT 
CCCCAGCAAA ACGTCCAGCC TAAGAAGCAG GTAAAGGGCA GGGCTGAGCC ACAGCCACCA 
GGGCCAGTCA TGGGCCAAGG CCAGGGATGC CCACCTGCCT CACCGGCTGC CGTGCCTATG 
CTGTCCCAGG AGCTGCGACC ACCTTCAGTC GTCCAGCCCC AGCCCCTGGT GGTAGTAAAG 
GAGGAGAAGA TTCACTCACC AATCATTCGC AGCGAGCCTT TCAGCACCTC ACTTCGACCA 
GAGCCCCCCA AGCACCCAGA GAACATCAAG GCCCCAGTCC ACCTGCCCCA GCGGCCTGAG 
ATGAAGCCTG TAGACATAGG GAGGCCTGTG ATCCGGCCTC CAGAGCAGAG TGCACCCCCA 
CCAGGGGCCC CTGACAAGGA CAAACAGAAG CAGGAGCCAA AAACACCAGT GGCACCCAAA 
AAGGACCTGA AAATTAAGAA CATGGGCTCC TGGGCCAGCC TGGTACAGAA GCATCCGACC 
ACCCCATCCT CCACAGCCAA GTCCTCAAGT GACAGCTTTG AGCATTTCCG CCGTGCTGCT 
CGGGAGAAGG AGGAGAGGGA AAAGGCCCTG AAGGCTCAGG CTGAGCATGC AGAGAAGGAG 
AAGGAGCGGC TGAGGCAGGA GCGCATGAGG AGCCGAGAGG ATGAAGATGC GCTGGAACAG 
GCACGTCGAG CCCATGAGGA GGCACGACGG CGCCAGGAGC AGCAGCAGCA GCAGCAGCAG 
CAGAGACAGG AGCAGCAGCA GCAGCAGCAG CAGGCAGCTG CTGTGGCCGC TGCCTCTGCC 
CCCCAGGCCC AGAGCTCCCA GCCCCAGTCT ATGCTGGACC AGCAGAGGGA GTTGGCCCGG 
AAACGAGAGC AGGAGCGGAG GCGCAGGGAG GCAATGGCAG CTACAATTGA CATGAATTTC 
CAGAGTGATC TTTTGTCAAT ATTTGAAGAA AATCTTTTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brd4 ( XM_017317588.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017173077.1 Mus musculus bromodomain containing 4 (Brd4), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OMu53865
Clone ID Related Accession (Same CDS sequence)	XM_011246543.2 , XM_030249929.1 , XM_011246543.3 , XM_017317588.1 , XM_017317588.2
Accession Version	XM_017317588.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3762bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	bromodomain-containing protein 4 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017317588.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAAG CTCTGGAGAA GCTCTTCTTG CAAAAAATCA ATGAACTGCC TACAGAAGAA 
ACTGAGATCA TGATAGTCCA GGCAAAAGGA AGAGGACGAG GGAGGAAAGA AACAGGTAGG 
GCAGCAAAGC CTGGTGTATC CACGGTACCA AACACAACTC AAGCATCAAC TTCTCCGCAG 
ACCCAGACGC CTCAGCAGAA CCCTCCTCCA CCTGTGCAGG CCACAACTCA CCCCTTTCCT 
GCTGTCACCC CAGACCTCAT TGCCCAGCCT CCTGTCATGA CAATGGTGCC CCCTCAGCCA 
CTTCAGACTC CTTCACCGGT ACCCCCCCAG CCACCACCCC CACCTGCTCC AGTTCCACAG 
CCTGTGCAGA GTCACCCGCC CATCATTGCG ACCACCCCCC AGCCTGTGAA GACAAAGAAA 
GGGGTGAAGA GGAAAGCAGA TACCACCACC CCTACCACCA TCGACCCCAT TCATGAGCCA 
CCCTCACTGG CCCCAGAGCC CAAGACCGCC AAGCTGGGTC CTCGGCGGGA GAGCAGCAGA 
CCTGTGAAGC CTCCAAAGAA GGATGTACCG GACTCACAGC AGCACCCAGG GCCAGAGAAG 
AGCAGCAAGA TCTCTGAGCA GCTAAAGTGC TGCAGTGGCA TCCTCAAGGA GATGTTTGCC 
AAGAAACATG CTGCCTATGC CTGGCCTTTC TACAAGCCTG TGGATGTGGA GGCACTGGGT 
CTGCACGACT ACTGTGACAT CATCAAACAT CCCATGGACA TGAGCACAAT CAAGTCTAAA 
CTAGAGTCCC GAGAGTACAG AGATGCCCAG GAATTTGGTG CTGATGTCCG ATTGATGTTC 
TCCAACTGCT ACAAGTACAA CCCCCCTGAC CATGAAGTGG TAGCCATGGC TCGAAAACTC 
CAGGATGTGT TTGAAATGCG CTTTGCCAAG ATGCCTGATG AGCCTGAAGA GCCAGTTGTT 
ACAGTGTCCT CTCCTGCAGT GCCACCCCCT ACAAAGGTGG TAGCCCCACC CTCATCTAGT 
GACAGCAGCA GCGACAGTTC TTCCGACAGC GACAGTTCCA CTGACGACTC TGAGGAAGAG 
CGAGCCCAGC GGCTGGCTGA ACTCCAGGAA CAGCTCAAGG CCGTGCATGA GCAGCTTGCA 
GCCCTCTCAC AGCCCCAGCA GAACAAACCA AAGAAAAAGG AGAAGGACAA GAAGGAAAAG 
AAAAAGGAAA AGCACAAAAA GAAAGAAGAA GTGGAGGAAA ATAAAAAAAG CAAAACCAAG 
GAACTTCCTC CCAAAAAGAC AAAGAAAAAT AACAGCAGCA ACAGCAATGT GAGCAAGAAG 
GAACCAGTAC CCACGAAGAC CAAGCCGCCT CCCACATATG AATCAGAAGA GGAGGATAAG 
TGTAAGCCCA TGTCTTATGA GGAGAAGCGG CAGCTAAGTC TAGATATCAA CAAACTTCCT 
GGTGAGAAGC TAGGCCGTGT AGTACACATA ATTCAGTCAA GGGAACCATC ACTTAAAAAC 
TCCAACCCCG ATGAGATTGA GATTGACTTT GAGACCCTGA AGCCATCTAC ACTACGAGAG 
TTGGAGCGAT ATGTCACCTC CTGTTTGCGG AAGAAAAGGA AACCTCAAGC TGAAAAAGTT 
GACGTGATTG CTGGTTCTTC CAAGATGAAG GGATTCTCAT CCTCTGAGTC GGAGAGCACC 
AGCGAATCCA GCTCCTCTGA CAGTGAAGAC TCTGAAACAG AGATGGCTCC CAAGTCAAAA 
AAGAAGGGGC ACACTGGGAG GGACCAGAAA AAGCATCATC ACCATCACCA TCCACAGATG 
CAGCCAGCCC CAGCTCCTGT GCCCCAGCAG CCGCCCCCAC CTCCACAGCA GCCTCCTCCA 
CCCCCACCTC CGCAGCAGCA GCAGCAGCAA CCTCCACCCC CACCACCTCC GCCCTCCATG 
CCACAGCAGA CTGCCCCAGC GATGAAGTCC TCGCCCCCAC CCTTCATCAC TGCCCAGGTC 
CCCGTCCTGG AACCACAGCT GCCAGGCAGT GTCTTTGACC CTATTGGCCA CTTCACCCAG 
CCCATCTTGC ACCTGCCGCA GCCGGAGCTG CCTCCTCACC TGCCCCAGCC ACCTGAGCAC 
AGCACTCCAC CCCATCTCAA CCAGCATGCT GTGGTCTCTC CTCCAGCTTT GCACAATGCG 
CTGCCCCAAC AGCCATCTCG GCCCAGTAAC CGAGCTGCTG CTCTGCCCCC AAAGCCTACC 
CGACCCCCAG CTGTGTCCCC TGCCCTGGCC CAGCCCCCCC TGCTCCCACA ACCACCAATG 
GCTCAGCCCC CCCAAGTGCT GCTGGAGGAT GAAGAGCCAC CTGCCCCACC CCTCACCTCC 
ATGCAGATGC AGTTGTACCT ACAGCAGCTG CAGAAGGTGC AGCCTCCCAC ACCACTACTC 
CCTTCCGTGA AGGTGCAGTC CCAACCCCCA CCGCCTTTGC CGCCTCCACC CCACCCTTCT 
GTGCAGCAGC AGCAGCTCCA GCCACAGCCA CCGCCACCCC CACCTCCTCA GCCACAGCCA 
CCACCCCAGC AACAACACCA GCCTCCACCA CGACCAGTTC ACTTGCCATC CATGCCCTTT 
TCTGCTCATA TTCAGCAGCC CCCACCACCC CCAGGACAGC AACCTACTCA CCCACCCCCA 
GGACAGCAGC CCCCACCACC ACAGCCTGCC AAGCCCCAGC AAGTCATCCA GCATCACCCT 
TCCCCCCGGC ACCACAAGTC AGACCCCTAC TCAGCTGGTC ATCTTCGTGA GGCTCCCTCT 
CCGCTTATGA TACATTCCCC TCAGATGCCA CAGTTCCAGA GCCTGACCCA TCAGTCTCCT 
CCCCAGCAAA ACGTCCAGCC TAAGAAGCAG GTAAAGGGCA GGGCTGAGCC ACAGCCACCA 
GGGCCAGTCA TGGGCCAAGG CCAGGGATGC CCACCTGCCT CACCGGCTGC CGTGCCTATG 
CTGTCCCAGG AGCTGCGACC ACCTTCAGTC GTCCAGCCCC AGCCCCTGGT GGTAGTAAAG 
GAGGAGAAGA TTCACTCACC AATCATTCGC AGCGAGCCTT TCAGCACCTC ACTTCGACCA 
GAGCCCCCCA AGCACCCAGA GAACATCAAG GCCCCAGTCC ACCTGCCCCA GCGGCCTGAG 
ATGAAGCCTG TAGACATAGG GAGGCCTGTG ATCCGGCCTC CAGAGCAGAG TGCACCCCCA 
CCAGGGGCCC CTGACAAGGA CAAACAGAAG CAGGAGCCAA AAACACCAGT GGCACCCAAA 
AAGGACCTGA AAATTAAGAA CATGGGCTCC TGGGCCAGCC TGGTACAGAA GCATCCGACC 
ACCCCATCCT CCACAGCCAA GTCCTCAAGT GACAGCTTTG AGCATTTCCG CCGTGCTGCT 
CGGGAGAAGG AGGAGAGGGA AAAGGCCCTG AAGGCTCAGG CTGAGCATGC AGAGAAGGAG 
AAGGAGCGGC TGAGGCAGGA GCGCATGAGG AGCCGAGAGG ATGAAGATGC GCTGGAACAG 
GCACGTCGAG CCCATGAGGA GGCACGACGG CGCCAGGAGC AGCAGCAGCA GCAGCAGCAG 
CAGAGACAGG AGCAGCAGCA GCAGCAGCAG CAGGCAGCTG CTGTGGCCGC TGCCTCTGCC 
CCCCAGGCCC AGAGCTCCCA GCCCCAGTCT ATGCTGGACC AGCAGAGGGA GTTGGCCCGG 
AAACGAGAGC AGGAGCGGAG GCGCAGGGAG GCAATGGCAG CTACAATTGA CATGAATTTC 
CAGAGTGATC TTTTGTCAAT ATTTGAAGAA AATCTTTTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017173077.1
CDS	795..4556
Translation	MAEALEKLFLQKINELPTEETEIMIVQAKGRGRGRKETGRAAKPGVSTVPNTTQASTSPQTQTPQQNPPPPVQATTHPFPAVTPDLIAQPPVMTMVPPQPLQTPSPVPPQPPPPPAPVPQPVQSHPPIIATTPQPVKTKKGVKRKADTTTPTTIDPIHEPPSLAPEPKTAKLGPRRESSRPVKPPKKDVPDSQQHPGPEKSSKISEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYCDIIKHPMDMSTIKSKLESREYRDAQEFGADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPEEPVVTVSSPAVPPPTKVVAPPSSSDSSSDSSSDSDSSTDDSEEERAQRLAELQEQLKAVHEQLAALSQPQQNKPKKKEKDKKEKKKEKHKKKEEVEENKKSKTKELPPKKTKKNNSSNSNVSKKEPVPTKTKPPPTYESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVDVIAGSSKMKGFSSSESESTSESSSSDSEDSETEMAPKSKKKGHTGRDQKKHHHHHHPQMQPAPAPVPQQPPPPPQQPPPPPPPQQQQQQPPPPPPPPSMPQQTAPAMKSSPPPFITAQVPVLEPQLPGSVFDPIGHFTQPILHLPQPELPPHLPQPPEHSTPPHLNQHAVVSPPALHNALPQQPSRPSNRAAALPPKPTRPPAVSPALAQPPLLPQPPMAQPPQVLLEDEEPPAPPLTSMQMQLYLQQLQKVQPPTPLLPSVKVQSQPPPPLPPPPHPSVQQQQLQPQPPPPPPPQPQPPPQQQHQPPPRPVHLPSMPFSAHIQQPPPPPGQQPTHPPPGQQPPPPQPAKPQQVIQHHPSPRHHKSDPYSAGHLREAPSPLMIHSPQMPQFQSLTHQSPPQQNVQPKKQVKGRAEPQPPGPVMGQGQGCPPASPAAVPMLSQELRPPSVVQPQPLVVVKEEKIHSPIIRSEPFSTSLRPEPPKHPENIKAPVHLPQRPEMKPVDIGRPVIRPPEQSAPPPGAPDKDKQKQEPKTPVAPKKDLKIKNMGSWASLVQKHPTTPSSTAKSSSDSFEHFRRAAREKEEREKALKAQAEHAEKEKERLRQERMRSREDEDALEQARRAHEEARRRQEQQQQQQQQRQEQQQQQQQAAAVAAASAPQAQSSQPQSMLDQQRELARKREQERRRREAMAATIDMNFQSDLLSIFEENLF

Target ORF information:

RefSeq Version	XM_017317588.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus bromodomain containing 4 (Brd4), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317588.2

ORF Insert Sequence:

ATGGCAGAAG CTCTGGAGAA GCTCTTCTTG CAAAAAATCA ATGAACTGCC TACAGAAGAA 
ACTGAGATCA TGATAGTCCA GGCAAAAGGA AGAGGACGAG GGAGGAAAGA AACAGGTAGG 
GCAGCAAAGC CTGGTGTATC CACGGTACCA AACACAACTC AAGCATCAAC TTCTCCGCAG 
ACCCAGACGC CTCAGCAGAA CCCTCCTCCA CCTGTGCAGG CCACAACTCA CCCCTTTCCT 
GCTGTCACCC CAGACCTCAT TGCCCAGCCT CCTGTCATGA CAATGGTGCC CCCTCAGCCA 
CTTCAGACTC CTTCACCGGT ACCCCCCCAG CCACCACCCC CACCTGCTCC AGTTCCACAG 
CCTGTGCAGA GTCACCCGCC CATCATTGCG ACCACCCCCC AGCCTGTGAA GACAAAGAAA 
GGGGTGAAGA GGAAAGCAGA TACCACCACC CCTACCACCA TCGACCCCAT TCATGAGCCA 
CCCTCACTGG CCCCAGAGCC CAAGACCGCC AAGCTGGGTC CTCGGCGGGA GAGCAGCAGA 
CCTGTGAAGC CTCCAAAGAA GGATGTACCG GACTCACAGC AGCACCCAGG GCCAGAGAAG 
AGCAGCAAGA TCTCTGAGCA GCTAAAGTGC TGCAGTGGCA TCCTCAAGGA GATGTTTGCC 
AAGAAACATG CTGCCTATGC CTGGCCTTTC TACAAGCCTG TGGATGTGGA GGCACTGGGT 
CTGCACGACT ACTGTGACAT CATCAAACAT CCCATGGACA TGAGCACAAT CAAGTCTAAA 
CTAGAGTCCC GAGAGTACAG AGATGCCCAG GAATTTGGTG CTGATGTCCG ATTGATGTTC 
TCCAACTGCT ACAAGTACAA CCCCCCTGAC CATGAAGTGG TAGCCATGGC TCGAAAACTC 
CAGGATGTGT TTGAAATGCG CTTTGCCAAG ATGCCTGATG AGCCTGAAGA GCCAGTTGTT 
ACAGTGTCCT CTCCTGCAGT GCCACCCCCT ACAAAGGTGG TAGCCCCACC CTCATCTAGT 
GACAGCAGCA GCGACAGTTC TTCCGACAGC GACAGTTCCA CTGACGACTC TGAGGAAGAG 
CGAGCCCAGC GGCTGGCTGA ACTCCAGGAA CAGCTCAAGG CCGTGCATGA GCAGCTTGCA 
GCCCTCTCAC AGCCCCAGCA GAACAAACCA AAGAAAAAGG AGAAGGACAA GAAGGAAAAG 
AAAAAGGAAA AGCACAAAAA GAAAGAAGAA GTGGAGGAAA ATAAAAAAAG CAAAACCAAG 
GAACTTCCTC CCAAAAAGAC AAAGAAAAAT AACAGCAGCA ACAGCAATGT GAGCAAGAAG 
GAACCAGTAC CCACGAAGAC CAAGCCGCCT CCCACATATG AATCAGAAGA GGAGGATAAG 
TGTAAGCCCA TGTCTTATGA GGAGAAGCGG CAGCTAAGTC TAGATATCAA CAAACTTCCT 
GGTGAGAAGC TAGGCCGTGT AGTACACATA ATTCAGTCAA GGGAACCATC ACTTAAAAAC 
TCCAACCCCG ATGAGATTGA GATTGACTTT GAGACCCTGA AGCCATCTAC ACTACGAGAG 
TTGGAGCGAT ATGTCACCTC CTGTTTGCGG AAGAAAAGGA AACCTCAAGC TGAAAAAGTT 
GACGTGATTG CTGGTTCTTC CAAGATGAAG GGATTCTCAT CCTCTGAGTC GGAGAGCACC 
AGCGAATCCA GCTCCTCTGA CAGTGAAGAC TCTGAAACAG AGATGGCTCC CAAGTCAAAA 
AAGAAGGGGC ACACTGGGAG GGACCAGAAA AAGCATCATC ACCATCACCA TCCACAGATG 
CAGCCAGCCC CAGCTCCTGT GCCCCAGCAG CCGCCCCCAC CTCCACAGCA GCCTCCTCCA 
CCCCCACCTC CGCAGCAGCA GCAGCAGCAA CCTCCACCCC CACCACCTCC GCCCTCCATG 
CCACAGCAGA CTGCCCCAGC GATGAAGTCC TCGCCCCCAC CCTTCATCAC TGCCCAGGTC 
CCCGTCCTGG AACCACAGCT GCCAGGCAGT GTCTTTGACC CTATTGGCCA CTTCACCCAG 
CCCATCTTGC ACCTGCCGCA GCCGGAGCTG CCTCCTCACC TGCCCCAGCC ACCTGAGCAC 
AGCACTCCAC CCCATCTCAA CCAGCATGCT GTGGTCTCTC CTCCAGCTTT GCACAATGCG 
CTGCCCCAAC AGCCATCTCG GCCCAGTAAC CGAGCTGCTG CTCTGCCCCC AAAGCCTACC 
CGACCCCCAG CTGTGTCCCC TGCCCTGGCC CAGCCCCCCC TGCTCCCACA ACCACCAATG 
GCTCAGCCCC CCCAAGTGCT GCTGGAGGAT GAAGAGCCAC CTGCCCCACC CCTCACCTCC 
ATGCAGATGC AGTTGTACCT ACAGCAGCTG CAGAAGGTGC AGCCTCCCAC ACCACTACTC 
CCTTCCGTGA AGGTGCAGTC CCAACCCCCA CCGCCTTTGC CGCCTCCACC CCACCCTTCT 
GTGCAGCAGC AGCAGCTCCA GCCACAGCCA CCGCCACCCC CACCTCCTCA GCCACAGCCA 
CCACCCCAGC AACAACACCA GCCTCCACCA CGACCAGTTC ACTTGCCATC CATGCCCTTT 
TCTGCTCATA TTCAGCAGCC CCCACCACCC CCAGGACAGC AACCTACTCA CCCACCCCCA 
GGACAGCAGC CCCCACCACC ACAGCCTGCC AAGCCCCAGC AAGTCATCCA GCATCACCCT 
TCCCCCCGGC ACCACAAGTC AGACCCCTAC TCAGCTGGTC ATCTTCGTGA GGCTCCCTCT 
CCGCTTATGA TACATTCCCC TCAGATGCCA CAGTTCCAGA GCCTGACCCA TCAGTCTCCT 
CCCCAGCAAA ACGTCCAGCC TAAGAAGCAG GTAAAGGGCA GGGCTGAGCC ACAGCCACCA 
GGGCCAGTCA TGGGCCAAGG CCAGGGATGC CCACCTGCCT CACCGGCTGC CGTGCCTATG 
CTGTCCCAGG AGCTGCGACC ACCTTCAGTC GTCCAGCCCC AGCCCCTGGT GGTAGTAAAG 
GAGGAGAAGA TTCACTCACC AATCATTCGC AGCGAGCCTT TCAGCACCTC ACTTCGACCA 
GAGCCCCCCA AGCACCCAGA GAACATCAAG GCCCCAGTCC ACCTGCCCCA GCGGCCTGAG 
ATGAAGCCTG TAGACATAGG GAGGCCTGTG ATCCGGCCTC CAGAGCAGAG TGCACCCCCA 
CCAGGGGCCC CTGACAAGGA CAAACAGAAG CAGGAGCCAA AAACACCAGT GGCACCCAAA 
AAGGACCTGA AAATTAAGAA CATGGGCTCC TGGGCCAGCC TGGTACAGAA GCATCCGACC 
ACCCCATCCT CCACAGCCAA GTCCTCAAGT GACAGCTTTG AGCATTTCCG CCGTGCTGCT 
CGGGAGAAGG AGGAGAGGGA AAAGGCCCTG AAGGCTCAGG CTGAGCATGC AGAGAAGGAG 
AAGGAGCGGC TGAGGCAGGA GCGCATGAGG AGCCGAGAGG ATGAAGATGC GCTGGAACAG 
GCACGTCGAG CCCATGAGGA GGCACGACGG CGCCAGGAGC AGCAGCAGCA GCAGCAGCAG 
CAGAGACAGG AGCAGCAGCA GCAGCAGCAG CAGGCAGCTG CTGTGGCCGC TGCCTCTGCC 
CCCCAGGCCC AGAGCTCCCA GCCCCAGTCT ATGCTGGACC AGCAGAGGGA GTTGGCCCGG 
AAACGAGAGC AGGAGCGGAG GCGCAGGGAG GCAATGGCAG CTACAATTGA CATGAATTTC 
CAGAGTGATC TTTTGTCAAT ATTTGAAGAA AATCTTTTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brd4 ( XM_006524693.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524756.1 Mus musculus bromodomain containing 4 (Brd4), transcript variant X20, mRNA.

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CloneID	OMu53866
Clone ID Related Accession (Same CDS sequence)	XM_006524693.2 , XM_006524693.3 , XM_030249932.1 , XM_030249930.1 , XM_030249931.1
Accession Version	XM_006524693.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3759bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	bromodomain-containing protein 4 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006524693.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAAG CTCTGGAGAA GCTCTTCTTG CAAAAAATCA ATGAACTGCC TACAGAAGAA 
ACTGAGATCA TGATAGTCCA GGCAAAAGGA AGAGGACGAG GGAGGAAAGA AACAGGGGCA 
GCAAAGCCTG GTGTATCCAC GGTACCAAAC ACAACTCAAG CATCAACTTC TCCGCAGACC 
CAGACGCCTC AGCAGAACCC TCCTCCACCT GTGCAGGCCA CAACTCACCC CTTTCCTGCT 
GTCACCCCAG ACCTCATTGC CCAGCCTCCT GTCATGACAA TGGTGCCCCC TCAGCCACTT 
CAGACTCCTT CACCGGTACC CCCCCAGCCA CCACCCCCAC CTGCTCCAGT TCCACAGCCT 
GTGCAGAGTC ACCCGCCCAT CATTGCGACC ACCCCCCAGC CTGTGAAGAC AAAGAAAGGG 
GTGAAGAGGA AAGCAGATAC CACCACCCCT ACCACCATCG ACCCCATTCA TGAGCCACCC 
TCACTGGCCC CAGAGCCCAA GACCGCCAAG CTGGGTCCTC GGCGGGAGAG CAGCAGACCT 
GTGAAGCCTC CAAAGAAGGA TGTACCGGAC TCACAGCAGC ACCCAGGGCC AGAGAAGAGC 
AGCAAGATCT CTGAGCAGCT AAAGTGCTGC AGTGGCATCC TCAAGGAGAT GTTTGCCAAG 
AAACATGCTG CCTATGCCTG GCCTTTCTAC AAGCCTGTGG ATGTGGAGGC ACTGGGTCTG 
CACGACTACT GTGACATCAT CAAACATCCC ATGGACATGA GCACAATCAA GTCTAAACTA 
GAGTCCCGAG AGTACAGAGA TGCCCAGGAA TTTGGTGCTG ATGTCCGATT GATGTTCTCC 
AACTGCTACA AGTACAACCC CCCTGACCAT GAAGTGGTAG CCATGGCTCG AAAACTCCAG 
GATGTGTTTG AAATGCGCTT TGCCAAGATG CCTGATGAGC CTGAAGAGCC AGTTGTTACA 
GTGTCCTCTC CTGCAGTGCC ACCCCCTACA AAGGTGGTAG CCCCACCCTC ATCTAGTGAC 
AGCAGCAGCG ACAGTTCTTC CGACAGCGAC AGTTCCACTG ACGACTCTGA GGAAGAGCGA 
GCCCAGCGGC TGGCTGAACT CCAGGAACAG CTCAAGGCCG TGCATGAGCA GCTTGCAGCC 
CTCTCACAGC CCCAGCAGAA CAAACCAAAG AAAAAGGAGA AGGACAAGAA GGAAAAGAAA 
AAGGAAAAGC ACAAAAAGAA AGAAGAAGTG GAGGAAAATA AAAAAAGCAA AACCAAGGAA 
CTTCCTCCCA AAAAGACAAA GAAAAATAAC AGCAGCAACA GCAATGTGAG CAAGAAGGAA 
CCAGTACCCA CGAAGACCAA GCCGCCTCCC ACATATGAAT CAGAAGAGGA GGATAAGTGT 
AAGCCCATGT CTTATGAGGA GAAGCGGCAG CTAAGTCTAG ATATCAACAA ACTTCCTGGT 
GAGAAGCTAG GCCGTGTAGT ACACATAATT CAGTCAAGGG AACCATCACT TAAAAACTCC 
AACCCCGATG AGATTGAGAT TGACTTTGAG ACCCTGAAGC CATCTACACT ACGAGAGTTG 
GAGCGATATG TCACCTCCTG TTTGCGGAAG AAAAGGAAAC CTCAAGCTGA AAAAGTTGAC 
GTGATTGCTG GTTCTTCCAA GATGAAGGGA TTCTCATCCT CTGAGTCGGA GAGCACCAGC 
GAATCCAGCT CCTCTGACAG TGAAGACTCT GAAACAGAGA TGGCTCCCAA GTCAAAAAAG 
AAGGGGCACA CTGGGAGGGA CCAGAAAAAG CATCATCACC ATCACCATCC ACAGATGCAG 
CCAGCCCCAG CTCCTGTGCC CCAGCAGCCG CCCCCACCTC CACAGCAGCC TCCTCCACCC 
CCACCTCCGC AGCAGCAGCA GCAGCAACCT CCACCCCCAC CACCTCCGCC CTCCATGCCA 
CAGCAGACTG CCCCAGCGAT GAAGTCCTCG CCCCCACCCT TCATCACTGC CCAGGTCCCC 
GTCCTGGAAC CACAGCTGCC AGGCAGTGTC TTTGACCCTA TTGGCCACTT CACCCAGCCC 
ATCTTGCACC TGCCGCAGCC GGAGCTGCCT CCTCACCTGC CCCAGCCACC TGAGCACAGC 
ACTCCACCCC ATCTCAACCA GCATGCTGTG GTCTCTCCTC CAGCTTTGCA CAATGCGCTG 
CCCCAACAGC CATCTCGGCC CAGTAACCGA GCTGCTGCTC TGCCCCCAAA GCCTACCCGA 
CCCCCAGCTG TGTCCCCTGC CCTGGCCCAG CCCCCCCTGC TCCCACAACC ACCAATGGCT 
CAGCCCCCCC AAGTGCTGCT GGAGGATGAA GAGCCACCTG CCCCACCCCT CACCTCCATG 
CAGATGCAGT TGTACCTACA GCAGCTGCAG AAGGTGCAGC CTCCCACACC ACTACTCCCT 
TCCGTGAAGG TGCAGTCCCA ACCCCCACCG CCTTTGCCGC CTCCACCCCA CCCTTCTGTG 
CAGCAGCAGC AGCTCCAGCC ACAGCCACCG CCACCCCCAC CTCCTCAGCC ACAGCCACCA 
CCCCAGCAAC AACACCAGCC TCCACCACGA CCAGTTCACT TGCCATCCAT GCCCTTTTCT 
GCTCATATTC AGCAGCCCCC ACCACCCCCA GGACAGCAAC CTACTCACCC ACCCCCAGGA 
CAGCAGCCCC CACCACCACA GCCTGCCAAG CCCCAGCAAG TCATCCAGCA TCACCCTTCC 
CCCCGGCACC ACAAGTCAGA CCCCTACTCA GCTGGTCATC TTCGTGAGGC TCCCTCTCCG 
CTTATGATAC ATTCCCCTCA GATGCCACAG TTCCAGAGCC TGACCCATCA GTCTCCTCCC 
CAGCAAAACG TCCAGCCTAA GAAGCAGGTA AAGGGCAGGG CTGAGCCACA GCCACCAGGG 
CCAGTCATGG GCCAAGGCCA GGGATGCCCA CCTGCCTCAC CGGCTGCCGT GCCTATGCTG 
TCCCAGGAGC TGCGACCACC TTCAGTCGTC CAGCCCCAGC CCCTGGTGGT AGTAAAGGAG 
GAGAAGATTC ACTCACCAAT CATTCGCAGC GAGCCTTTCA GCACCTCACT TCGACCAGAG 
CCCCCCAAGC ACCCAGAGAA CATCAAGGCC CCAGTCCACC TGCCCCAGCG GCCTGAGATG 
AAGCCTGTAG ACATAGGGAG GCCTGTGATC CGGCCTCCAG AGCAGAGTGC ACCCCCACCA 
GGGGCCCCTG ACAAGGACAA ACAGAAGCAG GAGCCAAAAA CACCAGTGGC ACCCAAAAAG 
GACCTGAAAA TTAAGAACAT GGGCTCCTGG GCCAGCCTGG TACAGAAGCA TCCGACCACC 
CCATCCTCCA CAGCCAAGTC CTCAAGTGAC AGCTTTGAGC ATTTCCGCCG TGCTGCTCGG 
GAGAAGGAGG AGAGGGAAAA GGCCCTGAAG GCTCAGGCTG AGCATGCAGA GAAGGAGAAG 
GAGCGGCTGA GGCAGGAGCG CATGAGGAGC CGAGAGGATG AAGATGCGCT GGAACAGGCA 
CGTCGAGCCC ATGAGGAGGC ACGACGGCGC CAGGAGCAGC AGCAGCAGCA GCAGCAGCAG 
AGACAGGAGC AGCAGCAGCA GCAGCAGCAG GCAGCTGCTG TGGCCGCTGC CTCTGCCCCC 
CAGGCCCAGA GCTCCCAGCC CCAGTCTATG CTGGACCAGC AGAGGGAGTT GGCCCGGAAA 
CGAGAGCAGG AGCGGAGGCG CAGGGAGGCA ATGGCAGCTA CAATTGACAT GAATTTCCAG 
AGTGATCTTT TGTCAATATT TGAAGAAAAT CTTTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524756.1
CDS	836..4594
Translation	MAEALEKLFLQKINELPTEETEIMIVQAKGRGRGRKETGAAKPGVSTVPNTTQASTSPQTQTPQQNPPPPVQATTHPFPAVTPDLIAQPPVMTMVPPQPLQTPSPVPPQPPPPPAPVPQPVQSHPPIIATTPQPVKTKKGVKRKADTTTPTTIDPIHEPPSLAPEPKTAKLGPRRESSRPVKPPKKDVPDSQQHPGPEKSSKISEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYCDIIKHPMDMSTIKSKLESREYRDAQEFGADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPEEPVVTVSSPAVPPPTKVVAPPSSSDSSSDSSSDSDSSTDDSEEERAQRLAELQEQLKAVHEQLAALSQPQQNKPKKKEKDKKEKKKEKHKKKEEVEENKKSKTKELPPKKTKKNNSSNSNVSKKEPVPTKTKPPPTYESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVDVIAGSSKMKGFSSSESESTSESSSSDSEDSETEMAPKSKKKGHTGRDQKKHHHHHHPQMQPAPAPVPQQPPPPPQQPPPPPPPQQQQQQPPPPPPPPSMPQQTAPAMKSSPPPFITAQVPVLEPQLPGSVFDPIGHFTQPILHLPQPELPPHLPQPPEHSTPPHLNQHAVVSPPALHNALPQQPSRPSNRAAALPPKPTRPPAVSPALAQPPLLPQPPMAQPPQVLLEDEEPPAPPLTSMQMQLYLQQLQKVQPPTPLLPSVKVQSQPPPPLPPPPHPSVQQQQLQPQPPPPPPPQPQPPPQQQHQPPPRPVHLPSMPFSAHIQQPPPPPGQQPTHPPPGQQPPPPQPAKPQQVIQHHPSPRHHKSDPYSAGHLREAPSPLMIHSPQMPQFQSLTHQSPPQQNVQPKKQVKGRAEPQPPGPVMGQGQGCPPASPAAVPMLSQELRPPSVVQPQPLVVVKEEKIHSPIIRSEPFSTSLRPEPPKHPENIKAPVHLPQRPEMKPVDIGRPVIRPPEQSAPPPGAPDKDKQKQEPKTPVAPKKDLKIKNMGSWASLVQKHPTTPSSTAKSSSDSFEHFRRAAREKEEREKALKAQAEHAEKEKERLRQERMRSREDEDALEQARRAHEEARRRQEQQQQQQQQRQEQQQQQQQAAAVAAASAPQAQSSQPQSMLDQQRELARKREQERRRREAMAATIDMNFQSDLLSIFEENLF

Target ORF information:

RefSeq Version	XM_006524693.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus bromodomain containing 4 (Brd4), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524693.3

ORF Insert Sequence:

ATGGCAGAAG CTCTGGAGAA GCTCTTCTTG CAAAAAATCA ATGAACTGCC TACAGAAGAA 
ACTGAGATCA TGATAGTCCA GGCAAAAGGA AGAGGACGAG GGAGGAAAGA AACAGGGGCA 
GCAAAGCCTG GTGTATCCAC GGTACCAAAC ACAACTCAAG CATCAACTTC TCCGCAGACC 
CAGACGCCTC AGCAGAACCC TCCTCCACCT GTGCAGGCCA CAACTCACCC CTTTCCTGCT 
GTCACCCCAG ACCTCATTGC CCAGCCTCCT GTCATGACAA TGGTGCCCCC TCAGCCACTT 
CAGACTCCTT CACCGGTACC CCCCCAGCCA CCACCCCCAC CTGCTCCAGT TCCACAGCCT 
GTGCAGAGTC ACCCGCCCAT CATTGCGACC ACCCCCCAGC CTGTGAAGAC AAAGAAAGGG 
GTGAAGAGGA AAGCAGATAC CACCACCCCT ACCACCATCG ACCCCATTCA TGAGCCACCC 
TCACTGGCCC CAGAGCCCAA GACCGCCAAG CTGGGTCCTC GGCGGGAGAG CAGCAGACCT 
GTGAAGCCTC CAAAGAAGGA TGTACCGGAC TCACAGCAGC ACCCAGGGCC AGAGAAGAGC 
AGCAAGATCT CTGAGCAGCT AAAGTGCTGC AGTGGCATCC TCAAGGAGAT GTTTGCCAAG 
AAACATGCTG CCTATGCCTG GCCTTTCTAC AAGCCTGTGG ATGTGGAGGC ACTGGGTCTG 
CACGACTACT GTGACATCAT CAAACATCCC ATGGACATGA GCACAATCAA GTCTAAACTA 
GAGTCCCGAG AGTACAGAGA TGCCCAGGAA TTTGGTGCTG ATGTCCGATT GATGTTCTCC 
AACTGCTACA AGTACAACCC CCCTGACCAT GAAGTGGTAG CCATGGCTCG AAAACTCCAG 
GATGTGTTTG AAATGCGCTT TGCCAAGATG CCTGATGAGC CTGAAGAGCC AGTTGTTACA 
GTGTCCTCTC CTGCAGTGCC ACCCCCTACA AAGGTGGTAG CCCCACCCTC ATCTAGTGAC 
AGCAGCAGCG ACAGTTCTTC CGACAGCGAC AGTTCCACTG ACGACTCTGA GGAAGAGCGA 
GCCCAGCGGC TGGCTGAACT CCAGGAACAG CTCAAGGCCG TGCATGAGCA GCTTGCAGCC 
CTCTCACAGC CCCAGCAGAA CAAACCAAAG AAAAAGGAGA AGGACAAGAA GGAAAAGAAA 
AAGGAAAAGC ACAAAAAGAA AGAAGAAGTG GAGGAAAATA AAAAAAGCAA AACCAAGGAA 
CTTCCTCCCA AAAAGACAAA GAAAAATAAC AGCAGCAACA GCAATGTGAG CAAGAAGGAA 
CCAGTACCCA CGAAGACCAA GCCGCCTCCC ACATATGAAT CAGAAGAGGA GGATAAGTGT 
AAGCCCATGT CTTATGAGGA GAAGCGGCAG CTAAGTCTAG ATATCAACAA ACTTCCTGGT 
GAGAAGCTAG GCCGTGTAGT ACACATAATT CAGTCAAGGG AACCATCACT TAAAAACTCC 
AACCCCGATG AGATTGAGAT TGACTTTGAG ACCCTGAAGC CATCTACACT ACGAGAGTTG 
GAGCGATATG TCACCTCCTG TTTGCGGAAG AAAAGGAAAC CTCAAGCTGA AAAAGTTGAC 
GTGATTGCTG GTTCTTCCAA GATGAAGGGA TTCTCATCCT CTGAGTCGGA GAGCACCAGC 
GAATCCAGCT CCTCTGACAG TGAAGACTCT GAAACAGAGA TGGCTCCCAA GTCAAAAAAG 
AAGGGGCACA CTGGGAGGGA CCAGAAAAAG CATCATCACC ATCACCATCC ACAGATGCAG 
CCAGCCCCAG CTCCTGTGCC CCAGCAGCCG CCCCCACCTC CACAGCAGCC TCCTCCACCC 
CCACCTCCGC AGCAGCAGCA GCAGCAACCT CCACCCCCAC CACCTCCGCC CTCCATGCCA 
CAGCAGACTG CCCCAGCGAT GAAGTCCTCG CCCCCACCCT TCATCACTGC CCAGGTCCCC 
GTCCTGGAAC CACAGCTGCC AGGCAGTGTC TTTGACCCTA TTGGCCACTT CACCCAGCCC 
ATCTTGCACC TGCCGCAGCC GGAGCTGCCT CCTCACCTGC CCCAGCCACC TGAGCACAGC 
ACTCCACCCC ATCTCAACCA GCATGCTGTG GTCTCTCCTC CAGCTTTGCA CAATGCGCTG 
CCCCAACAGC CATCTCGGCC CAGTAACCGA GCTGCTGCTC TGCCCCCAAA GCCTACCCGA 
CCCCCAGCTG TGTCCCCTGC CCTGGCCCAG CCCCCCCTGC TCCCACAACC ACCAATGGCT 
CAGCCCCCCC AAGTGCTGCT GGAGGATGAA GAGCCACCTG CCCCACCCCT CACCTCCATG 
CAGATGCAGT TGTACCTACA GCAGCTGCAG AAGGTGCAGC CTCCCACACC ACTACTCCCT 
TCCGTGAAGG TGCAGTCCCA ACCCCCACCG CCTTTGCCGC CTCCACCCCA CCCTTCTGTG 
CAGCAGCAGC AGCTCCAGCC ACAGCCACCG CCACCCCCAC CTCCTCAGCC ACAGCCACCA 
CCCCAGCAAC AACACCAGCC TCCACCACGA CCAGTTCACT TGCCATCCAT GCCCTTTTCT 
GCTCATATTC AGCAGCCCCC ACCACCCCCA GGACAGCAAC CTACTCACCC ACCCCCAGGA 
CAGCAGCCCC CACCACCACA GCCTGCCAAG CCCCAGCAAG TCATCCAGCA TCACCCTTCC 
CCCCGGCACC ACAAGTCAGA CCCCTACTCA GCTGGTCATC TTCGTGAGGC TCCCTCTCCG 
CTTATGATAC ATTCCCCTCA GATGCCACAG TTCCAGAGCC TGACCCATCA GTCTCCTCCC 
CAGCAAAACG TCCAGCCTAA GAAGCAGGTA AAGGGCAGGG CTGAGCCACA GCCACCAGGG 
CCAGTCATGG GCCAAGGCCA GGGATGCCCA CCTGCCTCAC CGGCTGCCGT GCCTATGCTG 
TCCCAGGAGC TGCGACCACC TTCAGTCGTC CAGCCCCAGC CCCTGGTGGT AGTAAAGGAG 
GAGAAGATTC ACTCACCAAT CATTCGCAGC GAGCCTTTCA GCACCTCACT TCGACCAGAG 
CCCCCCAAGC ACCCAGAGAA CATCAAGGCC CCAGTCCACC TGCCCCAGCG GCCTGAGATG 
AAGCCTGTAG ACATAGGGAG GCCTGTGATC CGGCCTCCAG AGCAGAGTGC ACCCCCACCA 
GGGGCCCCTG ACAAGGACAA ACAGAAGCAG GAGCCAAAAA CACCAGTGGC ACCCAAAAAG 
GACCTGAAAA TTAAGAACAT GGGCTCCTGG GCCAGCCTGG TACAGAAGCA TCCGACCACC 
CCATCCTCCA CAGCCAAGTC CTCAAGTGAC AGCTTTGAGC ATTTCCGCCG TGCTGCTCGG 
GAGAAGGAGG AGAGGGAAAA GGCCCTGAAG GCTCAGGCTG AGCATGCAGA GAAGGAGAAG 
GAGCGGCTGA GGCAGGAGCG CATGAGGAGC CGAGAGGATG AAGATGCGCT GGAACAGGCA 
CGTCGAGCCC ATGAGGAGGC ACGACGGCGC CAGGAGCAGC AGCAGCAGCA GCAGCAGCAG 
AGACAGGAGC AGCAGCAGCA GCAGCAGCAG GCAGCTGCTG TGGCCGCTGC CTCTGCCCCC 
CAGGCCCAGA GCTCCCAGCC CCAGTCTATG CTGGACCAGC AGAGGGAGTT GGCCCGGAAA 
CGAGAGCAGG AGCGGAGGCG CAGGGAGGCA ATGGCAGCTA CAATTGACAT GAATTTCCAG 
AGTGATCTTT TGTCAATATT TGAAGAAAAT CTTTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brd4 ( XM_030249932.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105792.1 Mus musculus bromodomain containing 4 (Brd4), transcript variant X22, mRNA.

Price & Availability↑

CloneID	OMu53866
Clone ID Related Accession (Same CDS sequence)	XM_006524693.2 , XM_006524693.3 , XM_030249932.1 , XM_030249930.1 , XM_030249931.1
Accession Version	XM_030249932.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3759bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	bromodomain-containing protein 4 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAAG CTCTGGAGAA GCTCTTCTTG CAAAAAATCA ATGAACTGCC TACAGAAGAA 
ACTGAGATCA TGATAGTCCA GGCAAAAGGA AGAGGACGAG GGAGGAAAGA AACAGGGGCA 
GCAAAGCCTG GTGTATCCAC GGTACCAAAC ACAACTCAAG CATCAACTTC TCCGCAGACC 
CAGACGCCTC AGCAGAACCC TCCTCCACCT GTGCAGGCCA CAACTCACCC CTTTCCTGCT 
GTCACCCCAG ACCTCATTGC CCAGCCTCCT GTCATGACAA TGGTGCCCCC TCAGCCACTT 
CAGACTCCTT CACCGGTACC CCCCCAGCCA CCACCCCCAC CTGCTCCAGT TCCACAGCCT 
GTGCAGAGTC ACCCGCCCAT CATTGCGACC ACCCCCCAGC CTGTGAAGAC AAAGAAAGGG 
GTGAAGAGGA AAGCAGATAC CACCACCCCT ACCACCATCG ACCCCATTCA TGAGCCACCC 
TCACTGGCCC CAGAGCCCAA GACCGCCAAG CTGGGTCCTC GGCGGGAGAG CAGCAGACCT 
GTGAAGCCTC CAAAGAAGGA TGTACCGGAC TCACAGCAGC ACCCAGGGCC AGAGAAGAGC 
AGCAAGATCT CTGAGCAGCT AAAGTGCTGC AGTGGCATCC TCAAGGAGAT GTTTGCCAAG 
AAACATGCTG CCTATGCCTG GCCTTTCTAC AAGCCTGTGG ATGTGGAGGC ACTGGGTCTG 
CACGACTACT GTGACATCAT CAAACATCCC ATGGACATGA GCACAATCAA GTCTAAACTA 
GAGTCCCGAG AGTACAGAGA TGCCCAGGAA TTTGGTGCTG ATGTCCGATT GATGTTCTCC 
AACTGCTACA AGTACAACCC CCCTGACCAT GAAGTGGTAG CCATGGCTCG AAAACTCCAG 
GATGTGTTTG AAATGCGCTT TGCCAAGATG CCTGATGAGC CTGAAGAGCC AGTTGTTACA 
GTGTCCTCTC CTGCAGTGCC ACCCCCTACA AAGGTGGTAG CCCCACCCTC ATCTAGTGAC 
AGCAGCAGCG ACAGTTCTTC CGACAGCGAC AGTTCCACTG ACGACTCTGA GGAAGAGCGA 
GCCCAGCGGC TGGCTGAACT CCAGGAACAG CTCAAGGCCG TGCATGAGCA GCTTGCAGCC 
CTCTCACAGC CCCAGCAGAA CAAACCAAAG AAAAAGGAGA AGGACAAGAA GGAAAAGAAA 
AAGGAAAAGC ACAAAAAGAA AGAAGAAGTG GAGGAAAATA AAAAAAGCAA AACCAAGGAA 
CTTCCTCCCA AAAAGACAAA GAAAAATAAC AGCAGCAACA GCAATGTGAG CAAGAAGGAA 
CCAGTACCCA CGAAGACCAA GCCGCCTCCC ACATATGAAT CAGAAGAGGA GGATAAGTGT 
AAGCCCATGT CTTATGAGGA GAAGCGGCAG CTAAGTCTAG ATATCAACAA ACTTCCTGGT 
GAGAAGCTAG GCCGTGTAGT ACACATAATT CAGTCAAGGG AACCATCACT TAAAAACTCC 
AACCCCGATG AGATTGAGAT TGACTTTGAG ACCCTGAAGC CATCTACACT ACGAGAGTTG 
GAGCGATATG TCACCTCCTG TTTGCGGAAG AAAAGGAAAC CTCAAGCTGA AAAAGTTGAC 
GTGATTGCTG GTTCTTCCAA GATGAAGGGA TTCTCATCCT CTGAGTCGGA GAGCACCAGC 
GAATCCAGCT CCTCTGACAG TGAAGACTCT GAAACAGAGA TGGCTCCCAA GTCAAAAAAG 
AAGGGGCACA CTGGGAGGGA CCAGAAAAAG CATCATCACC ATCACCATCC ACAGATGCAG 
CCAGCCCCAG CTCCTGTGCC CCAGCAGCCG CCCCCACCTC CACAGCAGCC TCCTCCACCC 
CCACCTCCGC AGCAGCAGCA GCAGCAACCT CCACCCCCAC CACCTCCGCC CTCCATGCCA 
CAGCAGACTG CCCCAGCGAT GAAGTCCTCG CCCCCACCCT TCATCACTGC CCAGGTCCCC 
GTCCTGGAAC CACAGCTGCC AGGCAGTGTC TTTGACCCTA TTGGCCACTT CACCCAGCCC 
ATCTTGCACC TGCCGCAGCC GGAGCTGCCT CCTCACCTGC CCCAGCCACC TGAGCACAGC 
ACTCCACCCC ATCTCAACCA GCATGCTGTG GTCTCTCCTC CAGCTTTGCA CAATGCGCTG 
CCCCAACAGC CATCTCGGCC CAGTAACCGA GCTGCTGCTC TGCCCCCAAA GCCTACCCGA 
CCCCCAGCTG TGTCCCCTGC CCTGGCCCAG CCCCCCCTGC TCCCACAACC ACCAATGGCT 
CAGCCCCCCC AAGTGCTGCT GGAGGATGAA GAGCCACCTG CCCCACCCCT CACCTCCATG 
CAGATGCAGT TGTACCTACA GCAGCTGCAG AAGGTGCAGC CTCCCACACC ACTACTCCCT 
TCCGTGAAGG TGCAGTCCCA ACCCCCACCG CCTTTGCCGC CTCCACCCCA CCCTTCTGTG 
CAGCAGCAGC AGCTCCAGCC ACAGCCACCG CCACCCCCAC CTCCTCAGCC ACAGCCACCA 
CCCCAGCAAC AACACCAGCC TCCACCACGA CCAGTTCACT TGCCATCCAT GCCCTTTTCT 
GCTCATATTC AGCAGCCCCC ACCACCCCCA GGACAGCAAC CTACTCACCC ACCCCCAGGA 
CAGCAGCCCC CACCACCACA GCCTGCCAAG CCCCAGCAAG TCATCCAGCA TCACCCTTCC 
CCCCGGCACC ACAAGTCAGA CCCCTACTCA GCTGGTCATC TTCGTGAGGC TCCCTCTCCG 
CTTATGATAC ATTCCCCTCA GATGCCACAG TTCCAGAGCC TGACCCATCA GTCTCCTCCC 
CAGCAAAACG TCCAGCCTAA GAAGCAGGTA AAGGGCAGGG CTGAGCCACA GCCACCAGGG 
CCAGTCATGG GCCAAGGCCA GGGATGCCCA CCTGCCTCAC CGGCTGCCGT GCCTATGCTG 
TCCCAGGAGC TGCGACCACC TTCAGTCGTC CAGCCCCAGC CCCTGGTGGT AGTAAAGGAG 
GAGAAGATTC ACTCACCAAT CATTCGCAGC GAGCCTTTCA GCACCTCACT TCGACCAGAG 
CCCCCCAAGC ACCCAGAGAA CATCAAGGCC CCAGTCCACC TGCCCCAGCG GCCTGAGATG 
AAGCCTGTAG ACATAGGGAG GCCTGTGATC CGGCCTCCAG AGCAGAGTGC ACCCCCACCA 
GGGGCCCCTG ACAAGGACAA ACAGAAGCAG GAGCCAAAAA CACCAGTGGC ACCCAAAAAG 
GACCTGAAAA TTAAGAACAT GGGCTCCTGG GCCAGCCTGG TACAGAAGCA TCCGACCACC 
CCATCCTCCA CAGCCAAGTC CTCAAGTGAC AGCTTTGAGC ATTTCCGCCG TGCTGCTCGG 
GAGAAGGAGG AGAGGGAAAA GGCCCTGAAG GCTCAGGCTG AGCATGCAGA GAAGGAGAAG 
GAGCGGCTGA GGCAGGAGCG CATGAGGAGC CGAGAGGATG AAGATGCGCT GGAACAGGCA 
CGTCGAGCCC ATGAGGAGGC ACGACGGCGC CAGGAGCAGC AGCAGCAGCA GCAGCAGCAG 
AGACAGGAGC AGCAGCAGCA GCAGCAGCAG GCAGCTGCTG TGGCCGCTGC CTCTGCCCCC 
CAGGCCCAGA GCTCCCAGCC CCAGTCTATG CTGGACCAGC AGAGGGAGTT GGCCCGGAAA 
CGAGAGCAGG AGCGGAGGCG CAGGGAGGCA ATGGCAGCTA CAATTGACAT GAATTTCCAG 
AGTGATCTTT TGTCAATATT TGAAGAAAAT CTTTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105792.1
CDS	795..4553
Translation	MAEALEKLFLQKINELPTEETEIMIVQAKGRGRGRKETGAAKPGVSTVPNTTQASTSPQTQTPQQNPPPPVQATTHPFPAVTPDLIAQPPVMTMVPPQPLQTPSPVPPQPPPPPAPVPQPVQSHPPIIATTPQPVKTKKGVKRKADTTTPTTIDPIHEPPSLAPEPKTAKLGPRRESSRPVKPPKKDVPDSQQHPGPEKSSKISEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYCDIIKHPMDMSTIKSKLESREYRDAQEFGADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPEEPVVTVSSPAVPPPTKVVAPPSSSDSSSDSSSDSDSSTDDSEEERAQRLAELQEQLKAVHEQLAALSQPQQNKPKKKEKDKKEKKKEKHKKKEEVEENKKSKTKELPPKKTKKNNSSNSNVSKKEPVPTKTKPPPTYESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVDVIAGSSKMKGFSSSESESTSESSSSDSEDSETEMAPKSKKKGHTGRDQKKHHHHHHPQMQPAPAPVPQQPPPPPQQPPPPPPPQQQQQQPPPPPPPPSMPQQTAPAMKSSPPPFITAQVPVLEPQLPGSVFDPIGHFTQPILHLPQPELPPHLPQPPEHSTPPHLNQHAVVSPPALHNALPQQPSRPSNRAAALPPKPTRPPAVSPALAQPPLLPQPPMAQPPQVLLEDEEPPAPPLTSMQMQLYLQQLQKVQPPTPLLPSVKVQSQPPPPLPPPPHPSVQQQQLQPQPPPPPPPQPQPPPQQQHQPPPRPVHLPSMPFSAHIQQPPPPPGQQPTHPPPGQQPPPPQPAKPQQVIQHHPSPRHHKSDPYSAGHLREAPSPLMIHSPQMPQFQSLTHQSPPQQNVQPKKQVKGRAEPQPPGPVMGQGQGCPPASPAAVPMLSQELRPPSVVQPQPLVVVKEEKIHSPIIRSEPFSTSLRPEPPKHPENIKAPVHLPQRPEMKPVDIGRPVIRPPEQSAPPPGAPDKDKQKQEPKTPVAPKKDLKIKNMGSWASLVQKHPTTPSSTAKSSSDSFEHFRRAAREKEEREKALKAQAEHAEKEKERLRQERMRSREDEDALEQARRAHEEARRRQEQQQQQQQQRQEQQQQQQQAAAVAAASAPQAQSSQPQSMLDQQRELARKREQERRRREAMAATIDMNFQSDLLSIFEENLF

Target ORF information:

RefSeq Version	XM_030249932.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus bromodomain containing 4 (Brd4), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249932.1

ORF Insert Sequence:

ATGGCAGAAG CTCTGGAGAA GCTCTTCTTG CAAAAAATCA ATGAACTGCC TACAGAAGAA 
ACTGAGATCA TGATAGTCCA GGCAAAAGGA AGAGGACGAG GGAGGAAAGA AACAGGGGCA 
GCAAAGCCTG GTGTATCCAC GGTACCAAAC ACAACTCAAG CATCAACTTC TCCGCAGACC 
CAGACGCCTC AGCAGAACCC TCCTCCACCT GTGCAGGCCA CAACTCACCC CTTTCCTGCT 
GTCACCCCAG ACCTCATTGC CCAGCCTCCT GTCATGACAA TGGTGCCCCC TCAGCCACTT 
CAGACTCCTT CACCGGTACC CCCCCAGCCA CCACCCCCAC CTGCTCCAGT TCCACAGCCT 
GTGCAGAGTC ACCCGCCCAT CATTGCGACC ACCCCCCAGC CTGTGAAGAC AAAGAAAGGG 
GTGAAGAGGA AAGCAGATAC CACCACCCCT ACCACCATCG ACCCCATTCA TGAGCCACCC 
TCACTGGCCC CAGAGCCCAA GACCGCCAAG CTGGGTCCTC GGCGGGAGAG CAGCAGACCT 
GTGAAGCCTC CAAAGAAGGA TGTACCGGAC TCACAGCAGC ACCCAGGGCC AGAGAAGAGC 
AGCAAGATCT CTGAGCAGCT AAAGTGCTGC AGTGGCATCC TCAAGGAGAT GTTTGCCAAG 
AAACATGCTG CCTATGCCTG GCCTTTCTAC AAGCCTGTGG ATGTGGAGGC ACTGGGTCTG 
CACGACTACT GTGACATCAT CAAACATCCC ATGGACATGA GCACAATCAA GTCTAAACTA 
GAGTCCCGAG AGTACAGAGA TGCCCAGGAA TTTGGTGCTG ATGTCCGATT GATGTTCTCC 
AACTGCTACA AGTACAACCC CCCTGACCAT GAAGTGGTAG CCATGGCTCG AAAACTCCAG 
GATGTGTTTG AAATGCGCTT TGCCAAGATG CCTGATGAGC CTGAAGAGCC AGTTGTTACA 
GTGTCCTCTC CTGCAGTGCC ACCCCCTACA AAGGTGGTAG CCCCACCCTC ATCTAGTGAC 
AGCAGCAGCG ACAGTTCTTC CGACAGCGAC AGTTCCACTG ACGACTCTGA GGAAGAGCGA 
GCCCAGCGGC TGGCTGAACT CCAGGAACAG CTCAAGGCCG TGCATGAGCA GCTTGCAGCC 
CTCTCACAGC CCCAGCAGAA CAAACCAAAG AAAAAGGAGA AGGACAAGAA GGAAAAGAAA 
AAGGAAAAGC ACAAAAAGAA AGAAGAAGTG GAGGAAAATA AAAAAAGCAA AACCAAGGAA 
CTTCCTCCCA AAAAGACAAA GAAAAATAAC AGCAGCAACA GCAATGTGAG CAAGAAGGAA 
CCAGTACCCA CGAAGACCAA GCCGCCTCCC ACATATGAAT CAGAAGAGGA GGATAAGTGT 
AAGCCCATGT CTTATGAGGA GAAGCGGCAG CTAAGTCTAG ATATCAACAA ACTTCCTGGT 
GAGAAGCTAG GCCGTGTAGT ACACATAATT CAGTCAAGGG AACCATCACT TAAAAACTCC 
AACCCCGATG AGATTGAGAT TGACTTTGAG ACCCTGAAGC CATCTACACT ACGAGAGTTG 
GAGCGATATG TCACCTCCTG TTTGCGGAAG AAAAGGAAAC CTCAAGCTGA AAAAGTTGAC 
GTGATTGCTG GTTCTTCCAA GATGAAGGGA TTCTCATCCT CTGAGTCGGA GAGCACCAGC 
GAATCCAGCT CCTCTGACAG TGAAGACTCT GAAACAGAGA TGGCTCCCAA GTCAAAAAAG 
AAGGGGCACA CTGGGAGGGA CCAGAAAAAG CATCATCACC ATCACCATCC ACAGATGCAG 
CCAGCCCCAG CTCCTGTGCC CCAGCAGCCG CCCCCACCTC CACAGCAGCC TCCTCCACCC 
CCACCTCCGC AGCAGCAGCA GCAGCAACCT CCACCCCCAC CACCTCCGCC CTCCATGCCA 
CAGCAGACTG CCCCAGCGAT GAAGTCCTCG CCCCCACCCT TCATCACTGC CCAGGTCCCC 
GTCCTGGAAC CACAGCTGCC AGGCAGTGTC TTTGACCCTA TTGGCCACTT CACCCAGCCC 
ATCTTGCACC TGCCGCAGCC GGAGCTGCCT CCTCACCTGC CCCAGCCACC TGAGCACAGC 
ACTCCACCCC ATCTCAACCA GCATGCTGTG GTCTCTCCTC CAGCTTTGCA CAATGCGCTG 
CCCCAACAGC CATCTCGGCC CAGTAACCGA GCTGCTGCTC TGCCCCCAAA GCCTACCCGA 
CCCCCAGCTG TGTCCCCTGC CCTGGCCCAG CCCCCCCTGC TCCCACAACC ACCAATGGCT 
CAGCCCCCCC AAGTGCTGCT GGAGGATGAA GAGCCACCTG CCCCACCCCT CACCTCCATG 
CAGATGCAGT TGTACCTACA GCAGCTGCAG AAGGTGCAGC CTCCCACACC ACTACTCCCT 
TCCGTGAAGG TGCAGTCCCA ACCCCCACCG CCTTTGCCGC CTCCACCCCA CCCTTCTGTG 
CAGCAGCAGC AGCTCCAGCC ACAGCCACCG CCACCCCCAC CTCCTCAGCC ACAGCCACCA 
CCCCAGCAAC AACACCAGCC TCCACCACGA CCAGTTCACT TGCCATCCAT GCCCTTTTCT 
GCTCATATTC AGCAGCCCCC ACCACCCCCA GGACAGCAAC CTACTCACCC ACCCCCAGGA 
CAGCAGCCCC CACCACCACA GCCTGCCAAG CCCCAGCAAG TCATCCAGCA TCACCCTTCC 
CCCCGGCACC ACAAGTCAGA CCCCTACTCA GCTGGTCATC TTCGTGAGGC TCCCTCTCCG 
CTTATGATAC ATTCCCCTCA GATGCCACAG TTCCAGAGCC TGACCCATCA GTCTCCTCCC 
CAGCAAAACG TCCAGCCTAA GAAGCAGGTA AAGGGCAGGG CTGAGCCACA GCCACCAGGG 
CCAGTCATGG GCCAAGGCCA GGGATGCCCA CCTGCCTCAC CGGCTGCCGT GCCTATGCTG 
TCCCAGGAGC TGCGACCACC TTCAGTCGTC CAGCCCCAGC CCCTGGTGGT AGTAAAGGAG 
GAGAAGATTC ACTCACCAAT CATTCGCAGC GAGCCTTTCA GCACCTCACT TCGACCAGAG 
CCCCCCAAGC ACCCAGAGAA CATCAAGGCC CCAGTCCACC TGCCCCAGCG GCCTGAGATG 
AAGCCTGTAG ACATAGGGAG GCCTGTGATC CGGCCTCCAG AGCAGAGTGC ACCCCCACCA 
GGGGCCCCTG ACAAGGACAA ACAGAAGCAG GAGCCAAAAA CACCAGTGGC ACCCAAAAAG 
GACCTGAAAA TTAAGAACAT GGGCTCCTGG GCCAGCCTGG TACAGAAGCA TCCGACCACC 
CCATCCTCCA CAGCCAAGTC CTCAAGTGAC AGCTTTGAGC ATTTCCGCCG TGCTGCTCGG 
GAGAAGGAGG AGAGGGAAAA GGCCCTGAAG GCTCAGGCTG AGCATGCAGA GAAGGAGAAG 
GAGCGGCTGA GGCAGGAGCG CATGAGGAGC CGAGAGGATG AAGATGCGCT GGAACAGGCA 
CGTCGAGCCC ATGAGGAGGC ACGACGGCGC CAGGAGCAGC AGCAGCAGCA GCAGCAGCAG 
AGACAGGAGC AGCAGCAGCA GCAGCAGCAG GCAGCTGCTG TGGCCGCTGC CTCTGCCCCC 
CAGGCCCAGA GCTCCCAGCC CCAGTCTATG CTGGACCAGC AGAGGGAGTT GGCCCGGAAA 
CGAGAGCAGG AGCGGAGGCG CAGGGAGGCA ATGGCAGCTA CAATTGACAT GAATTTCCAG 
AGTGATCTTT TGTCAATATT TGAAGAAAAT CTTTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brd4 ( XM_030249930.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105790.1 Mus musculus bromodomain containing 4 (Brd4), transcript variant X19, mRNA.

Price & Availability↑

CloneID	OMu53866
Clone ID Related Accession (Same CDS sequence)	XM_006524693.2 , XM_006524693.3 , XM_030249932.1 , XM_030249930.1 , XM_030249931.1
Accession Version	XM_030249930.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3759bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	bromodomain-containing protein 4 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAAG CTCTGGAGAA GCTCTTCTTG CAAAAAATCA ATGAACTGCC TACAGAAGAA 
ACTGAGATCA TGATAGTCCA GGCAAAAGGA AGAGGACGAG GGAGGAAAGA AACAGGGGCA 
GCAAAGCCTG GTGTATCCAC GGTACCAAAC ACAACTCAAG CATCAACTTC TCCGCAGACC 
CAGACGCCTC AGCAGAACCC TCCTCCACCT GTGCAGGCCA CAACTCACCC CTTTCCTGCT 
GTCACCCCAG ACCTCATTGC CCAGCCTCCT GTCATGACAA TGGTGCCCCC TCAGCCACTT 
CAGACTCCTT CACCGGTACC CCCCCAGCCA CCACCCCCAC CTGCTCCAGT TCCACAGCCT 
GTGCAGAGTC ACCCGCCCAT CATTGCGACC ACCCCCCAGC CTGTGAAGAC AAAGAAAGGG 
GTGAAGAGGA AAGCAGATAC CACCACCCCT ACCACCATCG ACCCCATTCA TGAGCCACCC 
TCACTGGCCC CAGAGCCCAA GACCGCCAAG CTGGGTCCTC GGCGGGAGAG CAGCAGACCT 
GTGAAGCCTC CAAAGAAGGA TGTACCGGAC TCACAGCAGC ACCCAGGGCC AGAGAAGAGC 
AGCAAGATCT CTGAGCAGCT AAAGTGCTGC AGTGGCATCC TCAAGGAGAT GTTTGCCAAG 
AAACATGCTG CCTATGCCTG GCCTTTCTAC AAGCCTGTGG ATGTGGAGGC ACTGGGTCTG 
CACGACTACT GTGACATCAT CAAACATCCC ATGGACATGA GCACAATCAA GTCTAAACTA 
GAGTCCCGAG AGTACAGAGA TGCCCAGGAA TTTGGTGCTG ATGTCCGATT GATGTTCTCC 
AACTGCTACA AGTACAACCC CCCTGACCAT GAAGTGGTAG CCATGGCTCG AAAACTCCAG 
GATGTGTTTG AAATGCGCTT TGCCAAGATG CCTGATGAGC CTGAAGAGCC AGTTGTTACA 
GTGTCCTCTC CTGCAGTGCC ACCCCCTACA AAGGTGGTAG CCCCACCCTC ATCTAGTGAC 
AGCAGCAGCG ACAGTTCTTC CGACAGCGAC AGTTCCACTG ACGACTCTGA GGAAGAGCGA 
GCCCAGCGGC TGGCTGAACT CCAGGAACAG CTCAAGGCCG TGCATGAGCA GCTTGCAGCC 
CTCTCACAGC CCCAGCAGAA CAAACCAAAG AAAAAGGAGA AGGACAAGAA GGAAAAGAAA 
AAGGAAAAGC ACAAAAAGAA AGAAGAAGTG GAGGAAAATA AAAAAAGCAA AACCAAGGAA 
CTTCCTCCCA AAAAGACAAA GAAAAATAAC AGCAGCAACA GCAATGTGAG CAAGAAGGAA 
CCAGTACCCA CGAAGACCAA GCCGCCTCCC ACATATGAAT CAGAAGAGGA GGATAAGTGT 
AAGCCCATGT CTTATGAGGA GAAGCGGCAG CTAAGTCTAG ATATCAACAA ACTTCCTGGT 
GAGAAGCTAG GCCGTGTAGT ACACATAATT CAGTCAAGGG AACCATCACT TAAAAACTCC 
AACCCCGATG AGATTGAGAT TGACTTTGAG ACCCTGAAGC CATCTACACT ACGAGAGTTG 
GAGCGATATG TCACCTCCTG TTTGCGGAAG AAAAGGAAAC CTCAAGCTGA AAAAGTTGAC 
GTGATTGCTG GTTCTTCCAA GATGAAGGGA TTCTCATCCT CTGAGTCGGA GAGCACCAGC 
GAATCCAGCT CCTCTGACAG TGAAGACTCT GAAACAGAGA TGGCTCCCAA GTCAAAAAAG 
AAGGGGCACA CTGGGAGGGA CCAGAAAAAG CATCATCACC ATCACCATCC ACAGATGCAG 
CCAGCCCCAG CTCCTGTGCC CCAGCAGCCG CCCCCACCTC CACAGCAGCC TCCTCCACCC 
CCACCTCCGC AGCAGCAGCA GCAGCAACCT CCACCCCCAC CACCTCCGCC CTCCATGCCA 
CAGCAGACTG CCCCAGCGAT GAAGTCCTCG CCCCCACCCT TCATCACTGC CCAGGTCCCC 
GTCCTGGAAC CACAGCTGCC AGGCAGTGTC TTTGACCCTA TTGGCCACTT CACCCAGCCC 
ATCTTGCACC TGCCGCAGCC GGAGCTGCCT CCTCACCTGC CCCAGCCACC TGAGCACAGC 
ACTCCACCCC ATCTCAACCA GCATGCTGTG GTCTCTCCTC CAGCTTTGCA CAATGCGCTG 
CCCCAACAGC CATCTCGGCC CAGTAACCGA GCTGCTGCTC TGCCCCCAAA GCCTACCCGA 
CCCCCAGCTG TGTCCCCTGC CCTGGCCCAG CCCCCCCTGC TCCCACAACC ACCAATGGCT 
CAGCCCCCCC AAGTGCTGCT GGAGGATGAA GAGCCACCTG CCCCACCCCT CACCTCCATG 
CAGATGCAGT TGTACCTACA GCAGCTGCAG AAGGTGCAGC CTCCCACACC ACTACTCCCT 
TCCGTGAAGG TGCAGTCCCA ACCCCCACCG CCTTTGCCGC CTCCACCCCA CCCTTCTGTG 
CAGCAGCAGC AGCTCCAGCC ACAGCCACCG CCACCCCCAC CTCCTCAGCC ACAGCCACCA 
CCCCAGCAAC AACACCAGCC TCCACCACGA CCAGTTCACT TGCCATCCAT GCCCTTTTCT 
GCTCATATTC AGCAGCCCCC ACCACCCCCA GGACAGCAAC CTACTCACCC ACCCCCAGGA 
CAGCAGCCCC CACCACCACA GCCTGCCAAG CCCCAGCAAG TCATCCAGCA TCACCCTTCC 
CCCCGGCACC ACAAGTCAGA CCCCTACTCA GCTGGTCATC TTCGTGAGGC TCCCTCTCCG 
CTTATGATAC ATTCCCCTCA GATGCCACAG TTCCAGAGCC TGACCCATCA GTCTCCTCCC 
CAGCAAAACG TCCAGCCTAA GAAGCAGGTA AAGGGCAGGG CTGAGCCACA GCCACCAGGG 
CCAGTCATGG GCCAAGGCCA GGGATGCCCA CCTGCCTCAC CGGCTGCCGT GCCTATGCTG 
TCCCAGGAGC TGCGACCACC TTCAGTCGTC CAGCCCCAGC CCCTGGTGGT AGTAAAGGAG 
GAGAAGATTC ACTCACCAAT CATTCGCAGC GAGCCTTTCA GCACCTCACT TCGACCAGAG 
CCCCCCAAGC ACCCAGAGAA CATCAAGGCC CCAGTCCACC TGCCCCAGCG GCCTGAGATG 
AAGCCTGTAG ACATAGGGAG GCCTGTGATC CGGCCTCCAG AGCAGAGTGC ACCCCCACCA 
GGGGCCCCTG ACAAGGACAA ACAGAAGCAG GAGCCAAAAA CACCAGTGGC ACCCAAAAAG 
GACCTGAAAA TTAAGAACAT GGGCTCCTGG GCCAGCCTGG TACAGAAGCA TCCGACCACC 
CCATCCTCCA CAGCCAAGTC CTCAAGTGAC AGCTTTGAGC ATTTCCGCCG TGCTGCTCGG 
GAGAAGGAGG AGAGGGAAAA GGCCCTGAAG GCTCAGGCTG AGCATGCAGA GAAGGAGAAG 
GAGCGGCTGA GGCAGGAGCG CATGAGGAGC CGAGAGGATG AAGATGCGCT GGAACAGGCA 
CGTCGAGCCC ATGAGGAGGC ACGACGGCGC CAGGAGCAGC AGCAGCAGCA GCAGCAGCAG 
AGACAGGAGC AGCAGCAGCA GCAGCAGCAG GCAGCTGCTG TGGCCGCTGC CTCTGCCCCC 
CAGGCCCAGA GCTCCCAGCC CCAGTCTATG CTGGACCAGC AGAGGGAGTT GGCCCGGAAA 
CGAGAGCAGG AGCGGAGGCG CAGGGAGGCA ATGGCAGCTA CAATTGACAT GAATTTCCAG 
AGTGATCTTT TGTCAATATT TGAAGAAAAT CTTTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105790.1
CDS	681..4439
Translation	MAEALEKLFLQKINELPTEETEIMIVQAKGRGRGRKETGAAKPGVSTVPNTTQASTSPQTQTPQQNPPPPVQATTHPFPAVTPDLIAQPPVMTMVPPQPLQTPSPVPPQPPPPPAPVPQPVQSHPPIIATTPQPVKTKKGVKRKADTTTPTTIDPIHEPPSLAPEPKTAKLGPRRESSRPVKPPKKDVPDSQQHPGPEKSSKISEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYCDIIKHPMDMSTIKSKLESREYRDAQEFGADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPEEPVVTVSSPAVPPPTKVVAPPSSSDSSSDSSSDSDSSTDDSEEERAQRLAELQEQLKAVHEQLAALSQPQQNKPKKKEKDKKEKKKEKHKKKEEVEENKKSKTKELPPKKTKKNNSSNSNVSKKEPVPTKTKPPPTYESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVDVIAGSSKMKGFSSSESESTSESSSSDSEDSETEMAPKSKKKGHTGRDQKKHHHHHHPQMQPAPAPVPQQPPPPPQQPPPPPPPQQQQQQPPPPPPPPSMPQQTAPAMKSSPPPFITAQVPVLEPQLPGSVFDPIGHFTQPILHLPQPELPPHLPQPPEHSTPPHLNQHAVVSPPALHNALPQQPSRPSNRAAALPPKPTRPPAVSPALAQPPLLPQPPMAQPPQVLLEDEEPPAPPLTSMQMQLYLQQLQKVQPPTPLLPSVKVQSQPPPPLPPPPHPSVQQQQLQPQPPPPPPPQPQPPPQQQHQPPPRPVHLPSMPFSAHIQQPPPPPGQQPTHPPPGQQPPPPQPAKPQQVIQHHPSPRHHKSDPYSAGHLREAPSPLMIHSPQMPQFQSLTHQSPPQQNVQPKKQVKGRAEPQPPGPVMGQGQGCPPASPAAVPMLSQELRPPSVVQPQPLVVVKEEKIHSPIIRSEPFSTSLRPEPPKHPENIKAPVHLPQRPEMKPVDIGRPVIRPPEQSAPPPGAPDKDKQKQEPKTPVAPKKDLKIKNMGSWASLVQKHPTTPSSTAKSSSDSFEHFRRAAREKEEREKALKAQAEHAEKEKERLRQERMRSREDEDALEQARRAHEEARRRQEQQQQQQQQRQEQQQQQQQAAAVAAASAPQAQSSQPQSMLDQQRELARKREQERRRREAMAATIDMNFQSDLLSIFEENLF

Target ORF information:

RefSeq Version	XM_030249930.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus bromodomain containing 4 (Brd4), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249930.1

ORF Insert Sequence:

ATGGCAGAAG CTCTGGAGAA GCTCTTCTTG CAAAAAATCA ATGAACTGCC TACAGAAGAA 
ACTGAGATCA TGATAGTCCA GGCAAAAGGA AGAGGACGAG GGAGGAAAGA AACAGGGGCA 
GCAAAGCCTG GTGTATCCAC GGTACCAAAC ACAACTCAAG CATCAACTTC TCCGCAGACC 
CAGACGCCTC AGCAGAACCC TCCTCCACCT GTGCAGGCCA CAACTCACCC CTTTCCTGCT 
GTCACCCCAG ACCTCATTGC CCAGCCTCCT GTCATGACAA TGGTGCCCCC TCAGCCACTT 
CAGACTCCTT CACCGGTACC CCCCCAGCCA CCACCCCCAC CTGCTCCAGT TCCACAGCCT 
GTGCAGAGTC ACCCGCCCAT CATTGCGACC ACCCCCCAGC CTGTGAAGAC AAAGAAAGGG 
GTGAAGAGGA AAGCAGATAC CACCACCCCT ACCACCATCG ACCCCATTCA TGAGCCACCC 
TCACTGGCCC CAGAGCCCAA GACCGCCAAG CTGGGTCCTC GGCGGGAGAG CAGCAGACCT 
GTGAAGCCTC CAAAGAAGGA TGTACCGGAC TCACAGCAGC ACCCAGGGCC AGAGAAGAGC 
AGCAAGATCT CTGAGCAGCT AAAGTGCTGC AGTGGCATCC TCAAGGAGAT GTTTGCCAAG 
AAACATGCTG CCTATGCCTG GCCTTTCTAC AAGCCTGTGG ATGTGGAGGC ACTGGGTCTG 
CACGACTACT GTGACATCAT CAAACATCCC ATGGACATGA GCACAATCAA GTCTAAACTA 
GAGTCCCGAG AGTACAGAGA TGCCCAGGAA TTTGGTGCTG ATGTCCGATT GATGTTCTCC 
AACTGCTACA AGTACAACCC CCCTGACCAT GAAGTGGTAG CCATGGCTCG AAAACTCCAG 
GATGTGTTTG AAATGCGCTT TGCCAAGATG CCTGATGAGC CTGAAGAGCC AGTTGTTACA 
GTGTCCTCTC CTGCAGTGCC ACCCCCTACA AAGGTGGTAG CCCCACCCTC ATCTAGTGAC 
AGCAGCAGCG ACAGTTCTTC CGACAGCGAC AGTTCCACTG ACGACTCTGA GGAAGAGCGA 
GCCCAGCGGC TGGCTGAACT CCAGGAACAG CTCAAGGCCG TGCATGAGCA GCTTGCAGCC 
CTCTCACAGC CCCAGCAGAA CAAACCAAAG AAAAAGGAGA AGGACAAGAA GGAAAAGAAA 
AAGGAAAAGC ACAAAAAGAA AGAAGAAGTG GAGGAAAATA AAAAAAGCAA AACCAAGGAA 
CTTCCTCCCA AAAAGACAAA GAAAAATAAC AGCAGCAACA GCAATGTGAG CAAGAAGGAA 
CCAGTACCCA CGAAGACCAA GCCGCCTCCC ACATATGAAT CAGAAGAGGA GGATAAGTGT 
AAGCCCATGT CTTATGAGGA GAAGCGGCAG CTAAGTCTAG ATATCAACAA ACTTCCTGGT 
GAGAAGCTAG GCCGTGTAGT ACACATAATT CAGTCAAGGG AACCATCACT TAAAAACTCC 
AACCCCGATG AGATTGAGAT TGACTTTGAG ACCCTGAAGC CATCTACACT ACGAGAGTTG 
GAGCGATATG TCACCTCCTG TTTGCGGAAG AAAAGGAAAC CTCAAGCTGA AAAAGTTGAC 
GTGATTGCTG GTTCTTCCAA GATGAAGGGA TTCTCATCCT CTGAGTCGGA GAGCACCAGC 
GAATCCAGCT CCTCTGACAG TGAAGACTCT GAAACAGAGA TGGCTCCCAA GTCAAAAAAG 
AAGGGGCACA CTGGGAGGGA CCAGAAAAAG CATCATCACC ATCACCATCC ACAGATGCAG 
CCAGCCCCAG CTCCTGTGCC CCAGCAGCCG CCCCCACCTC CACAGCAGCC TCCTCCACCC 
CCACCTCCGC AGCAGCAGCA GCAGCAACCT CCACCCCCAC CACCTCCGCC CTCCATGCCA 
CAGCAGACTG CCCCAGCGAT GAAGTCCTCG CCCCCACCCT TCATCACTGC CCAGGTCCCC 
GTCCTGGAAC CACAGCTGCC AGGCAGTGTC TTTGACCCTA TTGGCCACTT CACCCAGCCC 
ATCTTGCACC TGCCGCAGCC GGAGCTGCCT CCTCACCTGC CCCAGCCACC TGAGCACAGC 
ACTCCACCCC ATCTCAACCA GCATGCTGTG GTCTCTCCTC CAGCTTTGCA CAATGCGCTG 
CCCCAACAGC CATCTCGGCC CAGTAACCGA GCTGCTGCTC TGCCCCCAAA GCCTACCCGA 
CCCCCAGCTG TGTCCCCTGC CCTGGCCCAG CCCCCCCTGC TCCCACAACC ACCAATGGCT 
CAGCCCCCCC AAGTGCTGCT GGAGGATGAA GAGCCACCTG CCCCACCCCT CACCTCCATG 
CAGATGCAGT TGTACCTACA GCAGCTGCAG AAGGTGCAGC CTCCCACACC ACTACTCCCT 
TCCGTGAAGG TGCAGTCCCA ACCCCCACCG CCTTTGCCGC CTCCACCCCA CCCTTCTGTG 
CAGCAGCAGC AGCTCCAGCC ACAGCCACCG CCACCCCCAC CTCCTCAGCC ACAGCCACCA 
CCCCAGCAAC AACACCAGCC TCCACCACGA CCAGTTCACT TGCCATCCAT GCCCTTTTCT 
GCTCATATTC AGCAGCCCCC ACCACCCCCA GGACAGCAAC CTACTCACCC ACCCCCAGGA 
CAGCAGCCCC CACCACCACA GCCTGCCAAG CCCCAGCAAG TCATCCAGCA TCACCCTTCC 
CCCCGGCACC ACAAGTCAGA CCCCTACTCA GCTGGTCATC TTCGTGAGGC TCCCTCTCCG 
CTTATGATAC ATTCCCCTCA GATGCCACAG TTCCAGAGCC TGACCCATCA GTCTCCTCCC 
CAGCAAAACG TCCAGCCTAA GAAGCAGGTA AAGGGCAGGG CTGAGCCACA GCCACCAGGG 
CCAGTCATGG GCCAAGGCCA GGGATGCCCA CCTGCCTCAC CGGCTGCCGT GCCTATGCTG 
TCCCAGGAGC TGCGACCACC TTCAGTCGTC CAGCCCCAGC CCCTGGTGGT AGTAAAGGAG 
GAGAAGATTC ACTCACCAAT CATTCGCAGC GAGCCTTTCA GCACCTCACT TCGACCAGAG 
CCCCCCAAGC ACCCAGAGAA CATCAAGGCC CCAGTCCACC TGCCCCAGCG GCCTGAGATG 
AAGCCTGTAG ACATAGGGAG GCCTGTGATC CGGCCTCCAG AGCAGAGTGC ACCCCCACCA 
GGGGCCCCTG ACAAGGACAA ACAGAAGCAG GAGCCAAAAA CACCAGTGGC ACCCAAAAAG 
GACCTGAAAA TTAAGAACAT GGGCTCCTGG GCCAGCCTGG TACAGAAGCA TCCGACCACC 
CCATCCTCCA CAGCCAAGTC CTCAAGTGAC AGCTTTGAGC ATTTCCGCCG TGCTGCTCGG 
GAGAAGGAGG AGAGGGAAAA GGCCCTGAAG GCTCAGGCTG AGCATGCAGA GAAGGAGAAG 
GAGCGGCTGA GGCAGGAGCG CATGAGGAGC CGAGAGGATG AAGATGCGCT GGAACAGGCA 
CGTCGAGCCC ATGAGGAGGC ACGACGGCGC CAGGAGCAGC AGCAGCAGCA GCAGCAGCAG 
AGACAGGAGC AGCAGCAGCA GCAGCAGCAG GCAGCTGCTG TGGCCGCTGC CTCTGCCCCC 
CAGGCCCAGA GCTCCCAGCC CCAGTCTATG CTGGACCAGC AGAGGGAGTT GGCCCGGAAA 
CGAGAGCAGG AGCGGAGGCG CAGGGAGGCA ATGGCAGCTA CAATTGACAT GAATTTCCAG 
AGTGATCTTT TGTCAATATT TGAAGAAAAT CTTTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brd4 ( XM_030249931.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105791.1 Mus musculus bromodomain containing 4 (Brd4), transcript variant X21, mRNA.

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CloneID	OMu53866
Clone ID Related Accession (Same CDS sequence)	XM_006524693.2 , XM_006524693.3 , XM_030249932.1 , XM_030249930.1 , XM_030249931.1
Accession Version	XM_030249931.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3759bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	bromodomain-containing protein 4 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAAG CTCTGGAGAA GCTCTTCTTG CAAAAAATCA ATGAACTGCC TACAGAAGAA 
ACTGAGATCA TGATAGTCCA GGCAAAAGGA AGAGGACGAG GGAGGAAAGA AACAGGGGCA 
GCAAAGCCTG GTGTATCCAC GGTACCAAAC ACAACTCAAG CATCAACTTC TCCGCAGACC 
CAGACGCCTC AGCAGAACCC TCCTCCACCT GTGCAGGCCA CAACTCACCC CTTTCCTGCT 
GTCACCCCAG ACCTCATTGC CCAGCCTCCT GTCATGACAA TGGTGCCCCC TCAGCCACTT 
CAGACTCCTT CACCGGTACC CCCCCAGCCA CCACCCCCAC CTGCTCCAGT TCCACAGCCT 
GTGCAGAGTC ACCCGCCCAT CATTGCGACC ACCCCCCAGC CTGTGAAGAC AAAGAAAGGG 
GTGAAGAGGA AAGCAGATAC CACCACCCCT ACCACCATCG ACCCCATTCA TGAGCCACCC 
TCACTGGCCC CAGAGCCCAA GACCGCCAAG CTGGGTCCTC GGCGGGAGAG CAGCAGACCT 
GTGAAGCCTC CAAAGAAGGA TGTACCGGAC TCACAGCAGC ACCCAGGGCC AGAGAAGAGC 
AGCAAGATCT CTGAGCAGCT AAAGTGCTGC AGTGGCATCC TCAAGGAGAT GTTTGCCAAG 
AAACATGCTG CCTATGCCTG GCCTTTCTAC AAGCCTGTGG ATGTGGAGGC ACTGGGTCTG 
CACGACTACT GTGACATCAT CAAACATCCC ATGGACATGA GCACAATCAA GTCTAAACTA 
GAGTCCCGAG AGTACAGAGA TGCCCAGGAA TTTGGTGCTG ATGTCCGATT GATGTTCTCC 
AACTGCTACA AGTACAACCC CCCTGACCAT GAAGTGGTAG CCATGGCTCG AAAACTCCAG 
GATGTGTTTG AAATGCGCTT TGCCAAGATG CCTGATGAGC CTGAAGAGCC AGTTGTTACA 
GTGTCCTCTC CTGCAGTGCC ACCCCCTACA AAGGTGGTAG CCCCACCCTC ATCTAGTGAC 
AGCAGCAGCG ACAGTTCTTC CGACAGCGAC AGTTCCACTG ACGACTCTGA GGAAGAGCGA 
GCCCAGCGGC TGGCTGAACT CCAGGAACAG CTCAAGGCCG TGCATGAGCA GCTTGCAGCC 
CTCTCACAGC CCCAGCAGAA CAAACCAAAG AAAAAGGAGA AGGACAAGAA GGAAAAGAAA 
AAGGAAAAGC ACAAAAAGAA AGAAGAAGTG GAGGAAAATA AAAAAAGCAA AACCAAGGAA 
CTTCCTCCCA AAAAGACAAA GAAAAATAAC AGCAGCAACA GCAATGTGAG CAAGAAGGAA 
CCAGTACCCA CGAAGACCAA GCCGCCTCCC ACATATGAAT CAGAAGAGGA GGATAAGTGT 
AAGCCCATGT CTTATGAGGA GAAGCGGCAG CTAAGTCTAG ATATCAACAA ACTTCCTGGT 
GAGAAGCTAG GCCGTGTAGT ACACATAATT CAGTCAAGGG AACCATCACT TAAAAACTCC 
AACCCCGATG AGATTGAGAT TGACTTTGAG ACCCTGAAGC CATCTACACT ACGAGAGTTG 
GAGCGATATG TCACCTCCTG TTTGCGGAAG AAAAGGAAAC CTCAAGCTGA AAAAGTTGAC 
GTGATTGCTG GTTCTTCCAA GATGAAGGGA TTCTCATCCT CTGAGTCGGA GAGCACCAGC 
GAATCCAGCT CCTCTGACAG TGAAGACTCT GAAACAGAGA TGGCTCCCAA GTCAAAAAAG 
AAGGGGCACA CTGGGAGGGA CCAGAAAAAG CATCATCACC ATCACCATCC ACAGATGCAG 
CCAGCCCCAG CTCCTGTGCC CCAGCAGCCG CCCCCACCTC CACAGCAGCC TCCTCCACCC 
CCACCTCCGC AGCAGCAGCA GCAGCAACCT CCACCCCCAC CACCTCCGCC CTCCATGCCA 
CAGCAGACTG CCCCAGCGAT GAAGTCCTCG CCCCCACCCT TCATCACTGC CCAGGTCCCC 
GTCCTGGAAC CACAGCTGCC AGGCAGTGTC TTTGACCCTA TTGGCCACTT CACCCAGCCC 
ATCTTGCACC TGCCGCAGCC GGAGCTGCCT CCTCACCTGC CCCAGCCACC TGAGCACAGC 
ACTCCACCCC ATCTCAACCA GCATGCTGTG GTCTCTCCTC CAGCTTTGCA CAATGCGCTG 
CCCCAACAGC CATCTCGGCC CAGTAACCGA GCTGCTGCTC TGCCCCCAAA GCCTACCCGA 
CCCCCAGCTG TGTCCCCTGC CCTGGCCCAG CCCCCCCTGC TCCCACAACC ACCAATGGCT 
CAGCCCCCCC AAGTGCTGCT GGAGGATGAA GAGCCACCTG CCCCACCCCT CACCTCCATG 
CAGATGCAGT TGTACCTACA GCAGCTGCAG AAGGTGCAGC CTCCCACACC ACTACTCCCT 
TCCGTGAAGG TGCAGTCCCA ACCCCCACCG CCTTTGCCGC CTCCACCCCA CCCTTCTGTG 
CAGCAGCAGC AGCTCCAGCC ACAGCCACCG CCACCCCCAC CTCCTCAGCC ACAGCCACCA 
CCCCAGCAAC AACACCAGCC TCCACCACGA CCAGTTCACT TGCCATCCAT GCCCTTTTCT 
GCTCATATTC AGCAGCCCCC ACCACCCCCA GGACAGCAAC CTACTCACCC ACCCCCAGGA 
CAGCAGCCCC CACCACCACA GCCTGCCAAG CCCCAGCAAG TCATCCAGCA TCACCCTTCC 
CCCCGGCACC ACAAGTCAGA CCCCTACTCA GCTGGTCATC TTCGTGAGGC TCCCTCTCCG 
CTTATGATAC ATTCCCCTCA GATGCCACAG TTCCAGAGCC TGACCCATCA GTCTCCTCCC 
CAGCAAAACG TCCAGCCTAA GAAGCAGGTA AAGGGCAGGG CTGAGCCACA GCCACCAGGG 
CCAGTCATGG GCCAAGGCCA GGGATGCCCA CCTGCCTCAC CGGCTGCCGT GCCTATGCTG 
TCCCAGGAGC TGCGACCACC TTCAGTCGTC CAGCCCCAGC CCCTGGTGGT AGTAAAGGAG 
GAGAAGATTC ACTCACCAAT CATTCGCAGC GAGCCTTTCA GCACCTCACT TCGACCAGAG 
CCCCCCAAGC ACCCAGAGAA CATCAAGGCC CCAGTCCACC TGCCCCAGCG GCCTGAGATG 
AAGCCTGTAG ACATAGGGAG GCCTGTGATC CGGCCTCCAG AGCAGAGTGC ACCCCCACCA 
GGGGCCCCTG ACAAGGACAA ACAGAAGCAG GAGCCAAAAA CACCAGTGGC ACCCAAAAAG 
GACCTGAAAA TTAAGAACAT GGGCTCCTGG GCCAGCCTGG TACAGAAGCA TCCGACCACC 
CCATCCTCCA CAGCCAAGTC CTCAAGTGAC AGCTTTGAGC ATTTCCGCCG TGCTGCTCGG 
GAGAAGGAGG AGAGGGAAAA GGCCCTGAAG GCTCAGGCTG AGCATGCAGA GAAGGAGAAG 
GAGCGGCTGA GGCAGGAGCG CATGAGGAGC CGAGAGGATG AAGATGCGCT GGAACAGGCA 
CGTCGAGCCC ATGAGGAGGC ACGACGGCGC CAGGAGCAGC AGCAGCAGCA GCAGCAGCAG 
AGACAGGAGC AGCAGCAGCA GCAGCAGCAG GCAGCTGCTG TGGCCGCTGC CTCTGCCCCC 
CAGGCCCAGA GCTCCCAGCC CCAGTCTATG CTGGACCAGC AGAGGGAGTT GGCCCGGAAA 
CGAGAGCAGG AGCGGAGGCG CAGGGAGGCA ATGGCAGCTA CAATTGACAT GAATTTCCAG 
AGTGATCTTT TGTCAATATT TGAAGAAAAT CTTTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105791.1
CDS	640..4398
Translation	MAEALEKLFLQKINELPTEETEIMIVQAKGRGRGRKETGAAKPGVSTVPNTTQASTSPQTQTPQQNPPPPVQATTHPFPAVTPDLIAQPPVMTMVPPQPLQTPSPVPPQPPPPPAPVPQPVQSHPPIIATTPQPVKTKKGVKRKADTTTPTTIDPIHEPPSLAPEPKTAKLGPRRESSRPVKPPKKDVPDSQQHPGPEKSSKISEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYCDIIKHPMDMSTIKSKLESREYRDAQEFGADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPEEPVVTVSSPAVPPPTKVVAPPSSSDSSSDSSSDSDSSTDDSEEERAQRLAELQEQLKAVHEQLAALSQPQQNKPKKKEKDKKEKKKEKHKKKEEVEENKKSKTKELPPKKTKKNNSSNSNVSKKEPVPTKTKPPPTYESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVDVIAGSSKMKGFSSSESESTSESSSSDSEDSETEMAPKSKKKGHTGRDQKKHHHHHHPQMQPAPAPVPQQPPPPPQQPPPPPPPQQQQQQPPPPPPPPSMPQQTAPAMKSSPPPFITAQVPVLEPQLPGSVFDPIGHFTQPILHLPQPELPPHLPQPPEHSTPPHLNQHAVVSPPALHNALPQQPSRPSNRAAALPPKPTRPPAVSPALAQPPLLPQPPMAQPPQVLLEDEEPPAPPLTSMQMQLYLQQLQKVQPPTPLLPSVKVQSQPPPPLPPPPHPSVQQQQLQPQPPPPPPPQPQPPPQQQHQPPPRPVHLPSMPFSAHIQQPPPPPGQQPTHPPPGQQPPPPQPAKPQQVIQHHPSPRHHKSDPYSAGHLREAPSPLMIHSPQMPQFQSLTHQSPPQQNVQPKKQVKGRAEPQPPGPVMGQGQGCPPASPAAVPMLSQELRPPSVVQPQPLVVVKEEKIHSPIIRSEPFSTSLRPEPPKHPENIKAPVHLPQRPEMKPVDIGRPVIRPPEQSAPPPGAPDKDKQKQEPKTPVAPKKDLKIKNMGSWASLVQKHPTTPSSTAKSSSDSFEHFRRAAREKEEREKALKAQAEHAEKEKERLRQERMRSREDEDALEQARRAHEEARRRQEQQQQQQQQRQEQQQQQQQAAAVAAASAPQAQSSQPQSMLDQQRELARKREQERRRREAMAATIDMNFQSDLLSIFEENLF

Target ORF information:

RefSeq Version	XM_030249931.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus bromodomain containing 4 (Brd4), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249931.1

ORF Insert Sequence:

ATGGCAGAAG CTCTGGAGAA GCTCTTCTTG CAAAAAATCA ATGAACTGCC TACAGAAGAA 
ACTGAGATCA TGATAGTCCA GGCAAAAGGA AGAGGACGAG GGAGGAAAGA AACAGGGGCA 
GCAAAGCCTG GTGTATCCAC GGTACCAAAC ACAACTCAAG CATCAACTTC TCCGCAGACC 
CAGACGCCTC AGCAGAACCC TCCTCCACCT GTGCAGGCCA CAACTCACCC CTTTCCTGCT 
GTCACCCCAG ACCTCATTGC CCAGCCTCCT GTCATGACAA TGGTGCCCCC TCAGCCACTT 
CAGACTCCTT CACCGGTACC CCCCCAGCCA CCACCCCCAC CTGCTCCAGT TCCACAGCCT 
GTGCAGAGTC ACCCGCCCAT CATTGCGACC ACCCCCCAGC CTGTGAAGAC AAAGAAAGGG 
GTGAAGAGGA AAGCAGATAC CACCACCCCT ACCACCATCG ACCCCATTCA TGAGCCACCC 
TCACTGGCCC CAGAGCCCAA GACCGCCAAG CTGGGTCCTC GGCGGGAGAG CAGCAGACCT 
GTGAAGCCTC CAAAGAAGGA TGTACCGGAC TCACAGCAGC ACCCAGGGCC AGAGAAGAGC 
AGCAAGATCT CTGAGCAGCT AAAGTGCTGC AGTGGCATCC TCAAGGAGAT GTTTGCCAAG 
AAACATGCTG CCTATGCCTG GCCTTTCTAC AAGCCTGTGG ATGTGGAGGC ACTGGGTCTG 
CACGACTACT GTGACATCAT CAAACATCCC ATGGACATGA GCACAATCAA GTCTAAACTA 
GAGTCCCGAG AGTACAGAGA TGCCCAGGAA TTTGGTGCTG ATGTCCGATT GATGTTCTCC 
AACTGCTACA AGTACAACCC CCCTGACCAT GAAGTGGTAG CCATGGCTCG AAAACTCCAG 
GATGTGTTTG AAATGCGCTT TGCCAAGATG CCTGATGAGC CTGAAGAGCC AGTTGTTACA 
GTGTCCTCTC CTGCAGTGCC ACCCCCTACA AAGGTGGTAG CCCCACCCTC ATCTAGTGAC 
AGCAGCAGCG ACAGTTCTTC CGACAGCGAC AGTTCCACTG ACGACTCTGA GGAAGAGCGA 
GCCCAGCGGC TGGCTGAACT CCAGGAACAG CTCAAGGCCG TGCATGAGCA GCTTGCAGCC 
CTCTCACAGC CCCAGCAGAA CAAACCAAAG AAAAAGGAGA AGGACAAGAA GGAAAAGAAA 
AAGGAAAAGC ACAAAAAGAA AGAAGAAGTG GAGGAAAATA AAAAAAGCAA AACCAAGGAA 
CTTCCTCCCA AAAAGACAAA GAAAAATAAC AGCAGCAACA GCAATGTGAG CAAGAAGGAA 
CCAGTACCCA CGAAGACCAA GCCGCCTCCC ACATATGAAT CAGAAGAGGA GGATAAGTGT 
AAGCCCATGT CTTATGAGGA GAAGCGGCAG CTAAGTCTAG ATATCAACAA ACTTCCTGGT 
GAGAAGCTAG GCCGTGTAGT ACACATAATT CAGTCAAGGG AACCATCACT TAAAAACTCC 
AACCCCGATG AGATTGAGAT TGACTTTGAG ACCCTGAAGC CATCTACACT ACGAGAGTTG 
GAGCGATATG TCACCTCCTG TTTGCGGAAG AAAAGGAAAC CTCAAGCTGA AAAAGTTGAC 
GTGATTGCTG GTTCTTCCAA GATGAAGGGA TTCTCATCCT CTGAGTCGGA GAGCACCAGC 
GAATCCAGCT CCTCTGACAG TGAAGACTCT GAAACAGAGA TGGCTCCCAA GTCAAAAAAG 
AAGGGGCACA CTGGGAGGGA CCAGAAAAAG CATCATCACC ATCACCATCC ACAGATGCAG 
CCAGCCCCAG CTCCTGTGCC CCAGCAGCCG CCCCCACCTC CACAGCAGCC TCCTCCACCC 
CCACCTCCGC AGCAGCAGCA GCAGCAACCT CCACCCCCAC CACCTCCGCC CTCCATGCCA 
CAGCAGACTG CCCCAGCGAT GAAGTCCTCG CCCCCACCCT TCATCACTGC CCAGGTCCCC 
GTCCTGGAAC CACAGCTGCC AGGCAGTGTC TTTGACCCTA TTGGCCACTT CACCCAGCCC 
ATCTTGCACC TGCCGCAGCC GGAGCTGCCT CCTCACCTGC CCCAGCCACC TGAGCACAGC 
ACTCCACCCC ATCTCAACCA GCATGCTGTG GTCTCTCCTC CAGCTTTGCA CAATGCGCTG 
CCCCAACAGC CATCTCGGCC CAGTAACCGA GCTGCTGCTC TGCCCCCAAA GCCTACCCGA 
CCCCCAGCTG TGTCCCCTGC CCTGGCCCAG CCCCCCCTGC TCCCACAACC ACCAATGGCT 
CAGCCCCCCC AAGTGCTGCT GGAGGATGAA GAGCCACCTG CCCCACCCCT CACCTCCATG 
CAGATGCAGT TGTACCTACA GCAGCTGCAG AAGGTGCAGC CTCCCACACC ACTACTCCCT 
TCCGTGAAGG TGCAGTCCCA ACCCCCACCG CCTTTGCCGC CTCCACCCCA CCCTTCTGTG 
CAGCAGCAGC AGCTCCAGCC ACAGCCACCG CCACCCCCAC CTCCTCAGCC ACAGCCACCA 
CCCCAGCAAC AACACCAGCC TCCACCACGA CCAGTTCACT TGCCATCCAT GCCCTTTTCT 
GCTCATATTC AGCAGCCCCC ACCACCCCCA GGACAGCAAC CTACTCACCC ACCCCCAGGA 
CAGCAGCCCC CACCACCACA GCCTGCCAAG CCCCAGCAAG TCATCCAGCA TCACCCTTCC 
CCCCGGCACC ACAAGTCAGA CCCCTACTCA GCTGGTCATC TTCGTGAGGC TCCCTCTCCG 
CTTATGATAC ATTCCCCTCA GATGCCACAG TTCCAGAGCC TGACCCATCA GTCTCCTCCC 
CAGCAAAACG TCCAGCCTAA GAAGCAGGTA AAGGGCAGGG CTGAGCCACA GCCACCAGGG 
CCAGTCATGG GCCAAGGCCA GGGATGCCCA CCTGCCTCAC CGGCTGCCGT GCCTATGCTG 
TCCCAGGAGC TGCGACCACC TTCAGTCGTC CAGCCCCAGC CCCTGGTGGT AGTAAAGGAG 
GAGAAGATTC ACTCACCAAT CATTCGCAGC GAGCCTTTCA GCACCTCACT TCGACCAGAG 
CCCCCCAAGC ACCCAGAGAA CATCAAGGCC CCAGTCCACC TGCCCCAGCG GCCTGAGATG 
AAGCCTGTAG ACATAGGGAG GCCTGTGATC CGGCCTCCAG AGCAGAGTGC ACCCCCACCA 
GGGGCCCCTG ACAAGGACAA ACAGAAGCAG GAGCCAAAAA CACCAGTGGC ACCCAAAAAG 
GACCTGAAAA TTAAGAACAT GGGCTCCTGG GCCAGCCTGG TACAGAAGCA TCCGACCACC 
CCATCCTCCA CAGCCAAGTC CTCAAGTGAC AGCTTTGAGC ATTTCCGCCG TGCTGCTCGG 
GAGAAGGAGG AGAGGGAAAA GGCCCTGAAG GCTCAGGCTG AGCATGCAGA GAAGGAGAAG 
GAGCGGCTGA GGCAGGAGCG CATGAGGAGC CGAGAGGATG AAGATGCGCT GGAACAGGCA 
CGTCGAGCCC ATGAGGAGGC ACGACGGCGC CAGGAGCAGC AGCAGCAGCA GCAGCAGCAG 
AGACAGGAGC AGCAGCAGCA GCAGCAGCAG GCAGCTGCTG TGGCCGCTGC CTCTGCCCCC 
CAGGCCCAGA GCTCCCAGCC CCAGTCTATG CTGGACCAGC AGAGGGAGTT GGCCCGGAAA 
CGAGAGCAGG AGCGGAGGCG CAGGGAGGCA ATGGCAGCTA CAATTGACAT GAATTTCCAG 
AGTGATCTTT TGTCAATATT TGAAGAAAAT CTTTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brd4 ( NM_020508.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_065254.3 Mus musculus bromodomain containing 4 (Brd4), transcript variant 1, mRNA.

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CloneID	OMu16383
Clone ID Related Accession (Same CDS sequence)	XM_030249927.1 , XM_030249925.1 , XM_006524689.2 , NM_020508.4 , XM_006524688.1 , XM_006524688.2 , XM_030249926.1 , XM_006524689.3 , XM_030249924.1
Accession Version	NM_020508.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4203bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	bromodomain-containing protein 4 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY779550.1, AK147545.1 and CJ192204.1. On Nov 8, 2013 this sequence version replaced NM_020508.3. Transcript Variant: This variant (1) has two alternate splice sites, one in the 5' UTR and one in the 5' coding region, compared to variant 3. The resulting isoform (1) lacks an internal aa, compared to isoform 3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF461395.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGCC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGACAGACA 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCCGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAAAAA 
ATCAATGAAC TGCCTACAGA AGAAACTGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGAGGGAGGA AAGAAACAGG GGCAGCAAAG CCTGGTGTAT CCACGGTACC AAACACAACT 
CAAGCATCAA CTTCTCCGCA GACCCAGACG CCTCAGCAGA ACCCTCCTCC ACCTGTGCAG 
GCCACAACTC ACCCCTTTCC TGCTGTCACC CCAGACCTCA TTGCCCAGCC TCCTGTCATG 
ACAATGGTGC CCCCTCAGCC ACTTCAGACT CCTTCACCGG TACCCCCCCA GCCACCACCC 
CCACCTGCTC CAGTTCCACA GCCTGTGCAG AGTCACCCGC CCATCATTGC GACCACCCCC 
CAGCCTGTGA AGACAAAGAA AGGGGTGAAG AGGAAAGCAG ATACCACCAC CCCTACCACC 
ATCGACCCCA TTCATGAGCC ACCCTCACTG GCCCCAGAGC CCAAGACCGC CAAGCTGGGT 
CCTCGGCGGG AGAGCAGCAG ACCTGTGAAG CCTCCAAAGA AGGATGTACC GGACTCACAG 
CAGCACCCAG GGCCAGAGAA GAGCAGCAAG ATCTCTGAGC AGCTAAAGTG CTGCAGTGGC 
ATCCTCAAGG AGATGTTTGC CAAGAAACAT GCTGCCTATG CCTGGCCTTT CTACAAGCCT 
GTGGATGTGG AGGCACTGGG TCTGCACGAC TACTGTGACA TCATCAAACA TCCCATGGAC 
ATGAGCACAA TCAAGTCTAA ACTAGAGTCC CGAGAGTACA GAGATGCCCA GGAATTTGGT 
GCTGATGTCC GATTGATGTT CTCCAACTGC TACAAGTACA ACCCCCCTGA CCATGAAGTG 
GTAGCCATGG CTCGAAAACT CCAGGATGTG TTTGAAATGC GCTTTGCCAA GATGCCTGAT 
GAGCCTGAAG AGCCAGTTGT TACAGTGTCC TCTCCTGCAG TGCCACCCCC TACAAAGGTG 
GTAGCCCCAC CCTCATCTAG TGACAGCAGC AGCGACAGTT CTTCCGACAG CGACAGTTCC 
ACTGACGACT CTGAGGAAGA GCGAGCCCAG CGGCTGGCTG AACTCCAGGA ACAGCTCAAG 
GCCGTGCATG AGCAGCTTGC AGCCCTCTCA CAGCCCCAGC AGAACAAACC AAAGAAAAAG 
GAGAAGGACA AGAAGGAAAA GAAAAAGGAA AAGCACAAAA AGAAAGAAGA AGTGGAGGAA 
AATAAAAAAA GCAAAACCAA GGAACTTCCT CCCAAAAAGA CAAAGAAAAA TAACAGCAGC 
AACAGCAATG TGAGCAAGAA GGAACCAGTA CCCACGAAGA CCAAGCCGCC TCCCACATAT 
GAATCAGAAG AGGAGGATAA GTGTAAGCCC ATGTCTTATG AGGAGAAGCG GCAGCTAAGT 
CTAGATATCA ACAAACTTCC TGGTGAGAAG CTAGGCCGTG TAGTACACAT AATTCAGTCA 
AGGGAACCAT CACTTAAAAA CTCCAACCCC GATGAGATTG AGATTGACTT TGAGACCCTG 
AAGCCATCTA CACTACGAGA GTTGGAGCGA TATGTCACCT CCTGTTTGCG GAAGAAAAGG 
AAACCTCAAG CTGAAAAAGT TGACGTGATT GCTGGTTCTT CCAAGATGAA GGGATTCTCA 
TCCTCTGAGT CGGAGAGCAC CAGCGAATCC AGCTCCTCTG ACAGTGAAGA CTCTGAAACA 
GAGATGGCTC CCAAGTCAAA AAAGAAGGGG CACACTGGGA GGGACCAGAA AAAGCATCAT 
CACCATCACC ATCCACAGAT GCAGCCAGCC CCAGCTCCTG TGCCCCAGCA GCCGCCCCCA 
CCTCCACAGC AGCCTCCTCC ACCCCCACCT CCGCAGCAGC AGCAGCAGCA ACCTCCACCC 
CCACCACCTC CGCCCTCCAT GCCACAGCAG ACTGCCCCAG CGATGAAGTC CTCGCCCCCA 
CCCTTCATCA CTGCCCAGGT CCCCGTCCTG GAACCACAGC TGCCAGGCAG TGTCTTTGAC 
CCTATTGGCC ACTTCACCCA GCCCATCTTG CACCTGCCGC AGCCGGAGCT GCCTCCTCAC 
CTGCCCCAGC CACCTGAGCA CAGCACTCCA CCCCATCTCA ACCAGCATGC TGTGGTCTCT 
CCTCCAGCTT TGCACAATGC GCTGCCCCAA CAGCCATCTC GGCCCAGTAA CCGAGCTGCT 
GCTCTGCCCC CAAAGCCTAC CCGACCCCCA GCTGTGTCCC CTGCCCTGGC CCAGCCCCCC 
CTGCTCCCAC AACCACCAAT GGCTCAGCCC CCCCAAGTGC TGCTGGAGGA TGAAGAGCCA 
CCTGCCCCAC CCCTCACCTC CATGCAGATG CAGTTGTACC TACAGCAGCT GCAGAAGGTG 
CAGCCTCCCA CACCACTACT CCCTTCCGTG AAGGTGCAGT CCCAACCCCC ACCGCCTTTG 
CCGCCTCCAC CCCACCCTTC TGTGCAGCAG CAGCAGCTCC AGCCACAGCC ACCGCCACCC 
CCACCTCCTC AGCCACAGCC ACCACCCCAG CAACAACACC AGCCTCCACC ACGACCAGTT 
CACTTGCCAT CCATGCCCTT TTCTGCTCAT ATTCAGCAGC CCCCACCACC CCCAGGACAG 
CAACCTACTC ACCCACCCCC AGGACAGCAG CCCCCACCAC CACAGCCTGC CAAGCCCCAG 
CAAGTCATCC AGCATCACCC TTCCCCCCGG CACCACAAGT CAGACCCCTA CTCAGCTGGT 
CATCTTCGTG AGGCTCCCTC TCCGCTTATG ATACATTCCC CTCAGATGCC ACAGTTCCAG 
AGCCTGACCC ATCAGTCTCC TCCCCAGCAA AACGTCCAGC CTAAGAAGCA GGTAAAGGGC 
AGGGCTGAGC CACAGCCACC AGGGCCAGTC ATGGGCCAAG GCCAGGGATG CCCACCTGCC 
TCACCGGCTG CCGTGCCTAT GCTGTCCCAG GAGCTGCGAC CACCTTCAGT CGTCCAGCCC 
CAGCCCCTGG TGGTAGTAAA GGAGGAGAAG ATTCACTCAC CAATCATTCG CAGCGAGCCT 
TTCAGCACCT CACTTCGACC AGAGCCCCCC AAGCACCCAG AGAACATCAA GGCCCCAGTC 
CACCTGCCCC AGCGGCCTGA GATGAAGCCT GTAGACATAG GGAGGCCTGT GATCCGGCCT 
CCAGAGCAGA GTGCACCCCC ACCAGGGGCC CCTGACAAGG ACAAACAGAA GCAGGAGCCA 
AAAACACCAG TGGCACCCAA AAAGGACCTG AAAATTAAGA ACATGGGCTC CTGGGCCAGC 
CTGGTACAGA AGCATCCGAC CACCCCATCC TCCACAGCCA AGTCCTCAAG TGACAGCTTT 
GAGCATTTCC GCCGTGCTGC TCGGGAGAAG GAGGAGAGGG AAAAGGCCCT GAAGGCTCAG 
GCTGAGCATG CAGAGAAGGA GAAGGAGCGG CTGAGGCAGG AGCGCATGAG GAGCCGAGAG 
GATGAAGATG CGCTGGAACA GGCACGTCGA GCCCATGAGG AGGCACGACG GCGCCAGGAG 
CAGCAGCAGC AGCAGCAGCA GCAGAGACAG GAGCAGCAGC AGCAGCAGCA GCAGGCAGCT 
GCTGTGGCCG CTGCCTCTGC CCCCCAGGCC CAGAGCTCCC AGCCCCAGTC TATGCTGGAC 
CAGCAGAGGG AGTTGGCCCG GAAACGAGAG CAGGAGCGGA GGCGCAGGGA GGCAATGGCA 
GCTACAATTG ACATGAATTT CCAGAGTGAT CTTTTGTCAA TATTTGAAGA AAATCTTTTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065254.3
CDS	266..4468
Translation	MSTESGPGTRLRNLPVMGDGLETSQMSTTQAQAQPQPANAASTNPPPPETSNPNKPKRQTNQLQYLLRVVLKTLWKHQFAWPFQQPVDAVKLNLPDYYKIIKTPMDMGTIKKRLENNYYWNAQECIQDFNTMFTNCYIYNKPGDDIVLMAEALEKLFLQKINELPTEETEIMIVQAKGRGRGRKETGAAKPGVSTVPNTTQASTSPQTQTPQQNPPPPVQATTHPFPAVTPDLIAQPPVMTMVPPQPLQTPSPVPPQPPPPPAPVPQPVQSHPPIIATTPQPVKTKKGVKRKADTTTPTTIDPIHEPPSLAPEPKTAKLGPRRESSRPVKPPKKDVPDSQQHPGPEKSSKISEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYCDIIKHPMDMSTIKSKLESREYRDAQEFGADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPEEPVVTVSSPAVPPPTKVVAPPSSSDSSSDSSSDSDSSTDDSEEERAQRLAELQEQLKAVHEQLAALSQPQQNKPKKKEKDKKEKKKEKHKKKEEVEENKKSKTKELPPKKTKKNNSSNSNVSKKEPVPTKTKPPPTYESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVDVIAGSSKMKGFSSSESESTSESSSSDSEDSETEMAPKSKKKGHTGRDQKKHHHHHHPQMQPAPAPVPQQPPPPPQQPPPPPPPQQQQQQPPPPPPPPSMPQQTAPAMKSSPPPFITAQVPVLEPQLPGSVFDPIGHFTQPILHLPQPELPPHLPQPPEHSTPPHLNQHAVVSPPALHNALPQQPSRPSNRAAALPPKPTRPPAVSPALAQPPLLPQPPMAQPPQVLLEDEEPPAPPLTSMQMQLYLQQLQKVQPPTPLLPSVKVQSQPPPPLPPPPHPSVQQQQLQPQPPPPPPPQPQPPPQQQHQPPPRPVHLPSMPFSAHIQQPPPPPGQQPTHPPPGQQPPPPQPAKPQQVIQHHPSPRHHKSDPYSAGHLREAPSPLMIHSPQMPQFQSLTHQSPPQQNVQPKKQVKGRAEPQPPGPVMGQGQGCPPASPAAVPMLSQELRPPSVVQPQPLVVVKEEKIHSPIIRSEPFSTSLRPEPPKHPENIKAPVHLPQRPEMKPVDIGRPVIRPPEQSAPPPGAPDKDKQKQEPKTPVAPKKDLKIKNMGSWASLVQKHPTTPSSTAKSSSDSFEHFRRAAREKEEREKALKAQAEHAEKEKERLRQERMRSREDEDALEQARRAHEEARRRQEQQQQQQQQRQEQQQQQQQAAAVAAASAPQAQSSQPQSMLDQQRELARKREQERRRREAMAATIDMNFQSDLLSIFEENLF

Target ORF information:

RefSeq Version	NM_020508.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus bromodomain containing 4 (Brd4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020508.4

ORF Insert Sequence:

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGCC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGACAGACA 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCCGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAAAAA 
ATCAATGAAC TGCCTACAGA AGAAACTGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGAGGGAGGA AAGAAACAGG GGCAGCAAAG CCTGGTGTAT CCACGGTACC AAACACAACT 
CAAGCATCAA CTTCTCCGCA GACCCAGACG CCTCAGCAGA ACCCTCCTCC ACCTGTGCAG 
GCCACAACTC ACCCCTTTCC TGCTGTCACC CCAGACCTCA TTGCCCAGCC TCCTGTCATG 
ACAATGGTGC CCCCTCAGCC ACTTCAGACT CCTTCACCGG TACCCCCCCA GCCACCACCC 
CCACCTGCTC CAGTTCCACA GCCTGTGCAG AGTCACCCGC CCATCATTGC GACCACCCCC 
CAGCCTGTGA AGACAAAGAA AGGGGTGAAG AGGAAAGCAG ATACCACCAC CCCTACCACC 
ATCGACCCCA TTCATGAGCC ACCCTCACTG GCCCCAGAGC CCAAGACCGC CAAGCTGGGT 
CCTCGGCGGG AGAGCAGCAG ACCTGTGAAG CCTCCAAAGA AGGATGTACC GGACTCACAG 
CAGCACCCAG GGCCAGAGAA GAGCAGCAAG ATCTCTGAGC AGCTAAAGTG CTGCAGTGGC 
ATCCTCAAGG AGATGTTTGC CAAGAAACAT GCTGCCTATG CCTGGCCTTT CTACAAGCCT 
GTGGATGTGG AGGCACTGGG TCTGCACGAC TACTGTGACA TCATCAAACA TCCCATGGAC 
ATGAGCACAA TCAAGTCTAA ACTAGAGTCC CGAGAGTACA GAGATGCCCA GGAATTTGGT 
GCTGATGTCC GATTGATGTT CTCCAACTGC TACAAGTACA ACCCCCCTGA CCATGAAGTG 
GTAGCCATGG CTCGAAAACT CCAGGATGTG TTTGAAATGC GCTTTGCCAA GATGCCTGAT 
GAGCCTGAAG AGCCAGTTGT TACAGTGTCC TCTCCTGCAG TGCCACCCCC TACAAAGGTG 
GTAGCCCCAC CCTCATCTAG TGACAGCAGC AGCGACAGTT CTTCCGACAG CGACAGTTCC 
ACTGACGACT CTGAGGAAGA GCGAGCCCAG CGGCTGGCTG AACTCCAGGA ACAGCTCAAG 
GCCGTGCATG AGCAGCTTGC AGCCCTCTCA CAGCCCCAGC AGAACAAACC AAAGAAAAAG 
GAGAAGGACA AGAAGGAAAA GAAAAAGGAA AAGCACAAAA AGAAAGAAGA AGTGGAGGAA 
AATAAAAAAA GCAAAACCAA GGAACTTCCT CCCAAAAAGA CAAAGAAAAA TAACAGCAGC 
AACAGCAATG TGAGCAAGAA GGAACCAGTA CCCACGAAGA CCAAGCCGCC TCCCACATAT 
GAATCAGAAG AGGAGGATAA GTGTAAGCCC ATGTCTTATG AGGAGAAGCG GCAGCTAAGT 
CTAGATATCA ACAAACTTCC TGGTGAGAAG CTAGGCCGTG TAGTACACAT AATTCAGTCA 
AGGGAACCAT CACTTAAAAA CTCCAACCCC GATGAGATTG AGATTGACTT TGAGACCCTG 
AAGCCATCTA CACTACGAGA GTTGGAGCGA TATGTCACCT CCTGTTTGCG GAAGAAAAGG 
AAACCTCAAG CTGAAAAAGT TGACGTGATT GCTGGTTCTT CCAAGATGAA GGGATTCTCA 
TCCTCTGAGT CGGAGAGCAC CAGCGAATCC AGCTCCTCTG ACAGTGAAGA CTCTGAAACA 
GAGATGGCTC CCAAGTCAAA AAAGAAGGGG CACACTGGGA GGGACCAGAA AAAGCATCAT 
CACCATCACC ATCCACAGAT GCAGCCAGCC CCAGCTCCTG TGCCCCAGCA GCCGCCCCCA 
CCTCCACAGC AGCCTCCTCC ACCCCCACCT CCGCAGCAGC AGCAGCAGCA ACCTCCACCC 
CCACCACCTC CGCCCTCCAT GCCACAGCAG ACTGCCCCAG CGATGAAGTC CTCGCCCCCA 
CCCTTCATCA CTGCCCAGGT CCCCGTCCTG GAACCACAGC TGCCAGGCAG TGTCTTTGAC 
CCTATTGGCC ACTTCACCCA GCCCATCTTG CACCTGCCGC AGCCGGAGCT GCCTCCTCAC 
CTGCCCCAGC CACCTGAGCA CAGCACTCCA CCCCATCTCA ACCAGCATGC TGTGGTCTCT 
CCTCCAGCTT TGCACAATGC GCTGCCCCAA CAGCCATCTC GGCCCAGTAA CCGAGCTGCT 
GCTCTGCCCC CAAAGCCTAC CCGACCCCCA GCTGTGTCCC CTGCCCTGGC CCAGCCCCCC 
CTGCTCCCAC AACCACCAAT GGCTCAGCCC CCCCAAGTGC TGCTGGAGGA TGAAGAGCCA 
CCTGCCCCAC CCCTCACCTC CATGCAGATG CAGTTGTACC TACAGCAGCT GCAGAAGGTG 
CAGCCTCCCA CACCACTACT CCCTTCCGTG AAGGTGCAGT CCCAACCCCC ACCGCCTTTG 
CCGCCTCCAC CCCACCCTTC TGTGCAGCAG CAGCAGCTCC AGCCACAGCC ACCGCCACCC 
CCACCTCCTC AGCCACAGCC ACCACCCCAG CAACAACACC AGCCTCCACC ACGACCAGTT 
CACTTGCCAT CCATGCCCTT TTCTGCTCAT ATTCAGCAGC CCCCACCACC CCCAGGACAG 
CAACCTACTC ACCCACCCCC AGGACAGCAG CCCCCACCAC CACAGCCTGC CAAGCCCCAG 
CAAGTCATCC AGCATCACCC TTCCCCCCGG CACCACAAGT CAGACCCCTA CTCAGCTGGT 
CATCTTCGTG AGGCTCCCTC TCCGCTTATG ATACATTCCC CTCAGATGCC ACAGTTCCAG 
AGCCTGACCC ATCAGTCTCC TCCCCAGCAA AACGTCCAGC CTAAGAAGCA GGTAAAGGGC 
AGGGCTGAGC CACAGCCACC AGGGCCAGTC ATGGGCCAAG GCCAGGGATG CCCACCTGCC 
TCACCGGCTG CCGTGCCTAT GCTGTCCCAG GAGCTGCGAC CACCTTCAGT CGTCCAGCCC 
CAGCCCCTGG TGGTAGTAAA GGAGGAGAAG ATTCACTCAC CAATCATTCG CAGCGAGCCT 
TTCAGCACCT CACTTCGACC AGAGCCCCCC AAGCACCCAG AGAACATCAA GGCCCCAGTC 
CACCTGCCCC AGCGGCCTGA GATGAAGCCT GTAGACATAG GGAGGCCTGT GATCCGGCCT 
CCAGAGCAGA GTGCACCCCC ACCAGGGGCC CCTGACAAGG ACAAACAGAA GCAGGAGCCA 
AAAACACCAG TGGCACCCAA AAAGGACCTG AAAATTAAGA ACATGGGCTC CTGGGCCAGC 
CTGGTACAGA AGCATCCGAC CACCCCATCC TCCACAGCCA AGTCCTCAAG TGACAGCTTT 
GAGCATTTCC GCCGTGCTGC TCGGGAGAAG GAGGAGAGGG AAAAGGCCCT GAAGGCTCAG 
GCTGAGCATG CAGAGAAGGA GAAGGAGCGG CTGAGGCAGG AGCGCATGAG GAGCCGAGAG 
GATGAAGATG CGCTGGAACA GGCACGTCGA GCCCATGAGG AGGCACGACG GCGCCAGGAG 
CAGCAGCAGC AGCAGCAGCA GCAGAGACAG GAGCAGCAGC AGCAGCAGCA GCAGGCAGCT 
GCTGTGGCCG CTGCCTCTGC CCCCCAGGCC CAGAGCTCCC AGCCCCAGTC TATGCTGGAC 
CAGCAGAGGG AGTTGGCCCG GAAACGAGAG CAGGAGCGGA GGCGCAGGGA GGCAATGGCA 
GCTACAATTG ACATGAATTT CCAGAGTGAT CTTTTGTCAA TATTTGAAGA AAATCTTTTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brd4 ( XM_006524689.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524752.1 Mus musculus bromodomain containing 4 (Brd4), transcript variant X7, mRNA.

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CloneID	OMu16383
Clone ID Related Accession (Same CDS sequence)	XM_030249927.1 , XM_030249925.1 , XM_006524689.2 , NM_020508.4 , XM_006524688.1 , XM_006524688.2 , XM_030249926.1 , XM_006524689.3 , XM_030249924.1
Accession Version	XM_006524689.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4203bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	bromodomain-containing protein 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006524689.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGCC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGACAGACA 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCCGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAAAAA 
ATCAATGAAC TGCCTACAGA AGAAACTGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGAGGGAGGA AAGAAACAGG GGCAGCAAAG CCTGGTGTAT CCACGGTACC AAACACAACT 
CAAGCATCAA CTTCTCCGCA GACCCAGACG CCTCAGCAGA ACCCTCCTCC ACCTGTGCAG 
GCCACAACTC ACCCCTTTCC TGCTGTCACC CCAGACCTCA TTGCCCAGCC TCCTGTCATG 
ACAATGGTGC CCCCTCAGCC ACTTCAGACT CCTTCACCGG TACCCCCCCA GCCACCACCC 
CCACCTGCTC CAGTTCCACA GCCTGTGCAG AGTCACCCGC CCATCATTGC GACCACCCCC 
CAGCCTGTGA AGACAAAGAA AGGGGTGAAG AGGAAAGCAG ATACCACCAC CCCTACCACC 
ATCGACCCCA TTCATGAGCC ACCCTCACTG GCCCCAGAGC CCAAGACCGC CAAGCTGGGT 
CCTCGGCGGG AGAGCAGCAG ACCTGTGAAG CCTCCAAAGA AGGATGTACC GGACTCACAG 
CAGCACCCAG GGCCAGAGAA GAGCAGCAAG ATCTCTGAGC AGCTAAAGTG CTGCAGTGGC 
ATCCTCAAGG AGATGTTTGC CAAGAAACAT GCTGCCTATG CCTGGCCTTT CTACAAGCCT 
GTGGATGTGG AGGCACTGGG TCTGCACGAC TACTGTGACA TCATCAAACA TCCCATGGAC 
ATGAGCACAA TCAAGTCTAA ACTAGAGTCC CGAGAGTACA GAGATGCCCA GGAATTTGGT 
GCTGATGTCC GATTGATGTT CTCCAACTGC TACAAGTACA ACCCCCCTGA CCATGAAGTG 
GTAGCCATGG CTCGAAAACT CCAGGATGTG TTTGAAATGC GCTTTGCCAA GATGCCTGAT 
GAGCCTGAAG AGCCAGTTGT TACAGTGTCC TCTCCTGCAG TGCCACCCCC TACAAAGGTG 
GTAGCCCCAC CCTCATCTAG TGACAGCAGC AGCGACAGTT CTTCCGACAG CGACAGTTCC 
ACTGACGACT CTGAGGAAGA GCGAGCCCAG CGGCTGGCTG AACTCCAGGA ACAGCTCAAG 
GCCGTGCATG AGCAGCTTGC AGCCCTCTCA CAGCCCCAGC AGAACAAACC AAAGAAAAAG 
GAGAAGGACA AGAAGGAAAA GAAAAAGGAA AAGCACAAAA AGAAAGAAGA AGTGGAGGAA 
AATAAAAAAA GCAAAACCAA GGAACTTCCT CCCAAAAAGA CAAAGAAAAA TAACAGCAGC 
AACAGCAATG TGAGCAAGAA GGAACCAGTA CCCACGAAGA CCAAGCCGCC TCCCACATAT 
GAATCAGAAG AGGAGGATAA GTGTAAGCCC ATGTCTTATG AGGAGAAGCG GCAGCTAAGT 
CTAGATATCA ACAAACTTCC TGGTGAGAAG CTAGGCCGTG TAGTACACAT AATTCAGTCA 
AGGGAACCAT CACTTAAAAA CTCCAACCCC GATGAGATTG AGATTGACTT TGAGACCCTG 
AAGCCATCTA CACTACGAGA GTTGGAGCGA TATGTCACCT CCTGTTTGCG GAAGAAAAGG 
AAACCTCAAG CTGAAAAAGT TGACGTGATT GCTGGTTCTT CCAAGATGAA GGGATTCTCA 
TCCTCTGAGT CGGAGAGCAC CAGCGAATCC AGCTCCTCTG ACAGTGAAGA CTCTGAAACA 
GAGATGGCTC CCAAGTCAAA AAAGAAGGGG CACACTGGGA GGGACCAGAA AAAGCATCAT 
CACCATCACC ATCCACAGAT GCAGCCAGCC CCAGCTCCTG TGCCCCAGCA GCCGCCCCCA 
CCTCCACAGC AGCCTCCTCC ACCCCCACCT CCGCAGCAGC AGCAGCAGCA ACCTCCACCC 
CCACCACCTC CGCCCTCCAT GCCACAGCAG ACTGCCCCAG CGATGAAGTC CTCGCCCCCA 
CCCTTCATCA CTGCCCAGGT CCCCGTCCTG GAACCACAGC TGCCAGGCAG TGTCTTTGAC 
CCTATTGGCC ACTTCACCCA GCCCATCTTG CACCTGCCGC AGCCGGAGCT GCCTCCTCAC 
CTGCCCCAGC CACCTGAGCA CAGCACTCCA CCCCATCTCA ACCAGCATGC TGTGGTCTCT 
CCTCCAGCTT TGCACAATGC GCTGCCCCAA CAGCCATCTC GGCCCAGTAA CCGAGCTGCT 
GCTCTGCCCC CAAAGCCTAC CCGACCCCCA GCTGTGTCCC CTGCCCTGGC CCAGCCCCCC 
CTGCTCCCAC AACCACCAAT GGCTCAGCCC CCCCAAGTGC TGCTGGAGGA TGAAGAGCCA 
CCTGCCCCAC CCCTCACCTC CATGCAGATG CAGTTGTACC TACAGCAGCT GCAGAAGGTG 
CAGCCTCCCA CACCACTACT CCCTTCCGTG AAGGTGCAGT CCCAACCCCC ACCGCCTTTG 
CCGCCTCCAC CCCACCCTTC TGTGCAGCAG CAGCAGCTCC AGCCACAGCC ACCGCCACCC 
CCACCTCCTC AGCCACAGCC ACCACCCCAG CAACAACACC AGCCTCCACC ACGACCAGTT 
CACTTGCCAT CCATGCCCTT TTCTGCTCAT ATTCAGCAGC CCCCACCACC CCCAGGACAG 
CAACCTACTC ACCCACCCCC AGGACAGCAG CCCCCACCAC CACAGCCTGC CAAGCCCCAG 
CAAGTCATCC AGCATCACCC TTCCCCCCGG CACCACAAGT CAGACCCCTA CTCAGCTGGT 
CATCTTCGTG AGGCTCCCTC TCCGCTTATG ATACATTCCC CTCAGATGCC ACAGTTCCAG 
AGCCTGACCC ATCAGTCTCC TCCCCAGCAA AACGTCCAGC CTAAGAAGCA GGTAAAGGGC 
AGGGCTGAGC CACAGCCACC AGGGCCAGTC ATGGGCCAAG GCCAGGGATG CCCACCTGCC 
TCACCGGCTG CCGTGCCTAT GCTGTCCCAG GAGCTGCGAC CACCTTCAGT CGTCCAGCCC 
CAGCCCCTGG TGGTAGTAAA GGAGGAGAAG ATTCACTCAC CAATCATTCG CAGCGAGCCT 
TTCAGCACCT CACTTCGACC AGAGCCCCCC AAGCACCCAG AGAACATCAA GGCCCCAGTC 
CACCTGCCCC AGCGGCCTGA GATGAAGCCT GTAGACATAG GGAGGCCTGT GATCCGGCCT 
CCAGAGCAGA GTGCACCCCC ACCAGGGGCC CCTGACAAGG ACAAACAGAA GCAGGAGCCA 
AAAACACCAG TGGCACCCAA AAAGGACCTG AAAATTAAGA ACATGGGCTC CTGGGCCAGC 
CTGGTACAGA AGCATCCGAC CACCCCATCC TCCACAGCCA AGTCCTCAAG TGACAGCTTT 
GAGCATTTCC GCCGTGCTGC TCGGGAGAAG GAGGAGAGGG AAAAGGCCCT GAAGGCTCAG 
GCTGAGCATG CAGAGAAGGA GAAGGAGCGG CTGAGGCAGG AGCGCATGAG GAGCCGAGAG 
GATGAAGATG CGCTGGAACA GGCACGTCGA GCCCATGAGG AGGCACGACG GCGCCAGGAG 
CAGCAGCAGC AGCAGCAGCA GCAGAGACAG GAGCAGCAGC AGCAGCAGCA GCAGGCAGCT 
GCTGTGGCCG CTGCCTCTGC CCCCCAGGCC CAGAGCTCCC AGCCCCAGTC TATGCTGGAC 
CAGCAGAGGG AGTTGGCCCG GAAACGAGAG CAGGAGCGGA GGCGCAGGGA GGCAATGGCA 
GCTACAATTG ACATGAATTT CCAGAGTGAT CTTTTGTCAA TATTTGAAGA AAATCTTTTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524752.1
CDS	264..4466
Translation	MSTESGPGTRLRNLPVMGDGLETSQMSTTQAQAQPQPANAASTNPPPPETSNPNKPKRQTNQLQYLLRVVLKTLWKHQFAWPFQQPVDAVKLNLPDYYKIIKTPMDMGTIKKRLENNYYWNAQECIQDFNTMFTNCYIYNKPGDDIVLMAEALEKLFLQKINELPTEETEIMIVQAKGRGRGRKETGAAKPGVSTVPNTTQASTSPQTQTPQQNPPPPVQATTHPFPAVTPDLIAQPPVMTMVPPQPLQTPSPVPPQPPPPPAPVPQPVQSHPPIIATTPQPVKTKKGVKRKADTTTPTTIDPIHEPPSLAPEPKTAKLGPRRESSRPVKPPKKDVPDSQQHPGPEKSSKISEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYCDIIKHPMDMSTIKSKLESREYRDAQEFGADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPEEPVVTVSSPAVPPPTKVVAPPSSSDSSSDSSSDSDSSTDDSEEERAQRLAELQEQLKAVHEQLAALSQPQQNKPKKKEKDKKEKKKEKHKKKEEVEENKKSKTKELPPKKTKKNNSSNSNVSKKEPVPTKTKPPPTYESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVDVIAGSSKMKGFSSSESESTSESSSSDSEDSETEMAPKSKKKGHTGRDQKKHHHHHHPQMQPAPAPVPQQPPPPPQQPPPPPPPQQQQQQPPPPPPPPSMPQQTAPAMKSSPPPFITAQVPVLEPQLPGSVFDPIGHFTQPILHLPQPELPPHLPQPPEHSTPPHLNQHAVVSPPALHNALPQQPSRPSNRAAALPPKPTRPPAVSPALAQPPLLPQPPMAQPPQVLLEDEEPPAPPLTSMQMQLYLQQLQKVQPPTPLLPSVKVQSQPPPPLPPPPHPSVQQQQLQPQPPPPPPPQPQPPPQQQHQPPPRPVHLPSMPFSAHIQQPPPPPGQQPTHPPPGQQPPPPQPAKPQQVIQHHPSPRHHKSDPYSAGHLREAPSPLMIHSPQMPQFQSLTHQSPPQQNVQPKKQVKGRAEPQPPGPVMGQGQGCPPASPAAVPMLSQELRPPSVVQPQPLVVVKEEKIHSPIIRSEPFSTSLRPEPPKHPENIKAPVHLPQRPEMKPVDIGRPVIRPPEQSAPPPGAPDKDKQKQEPKTPVAPKKDLKIKNMGSWASLVQKHPTTPSSTAKSSSDSFEHFRRAAREKEEREKALKAQAEHAEKEKERLRQERMRSREDEDALEQARRAHEEARRRQEQQQQQQQQRQEQQQQQQQAAAVAAASAPQAQSSQPQSMLDQQRELARKREQERRRREAMAATIDMNFQSDLLSIFEENLF

Target ORF information:

RefSeq Version	XM_006524689.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus bromodomain containing 4 (Brd4), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524689.2

ORF Insert Sequence:

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGCC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGACAGACA 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCCGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAAAAA 
ATCAATGAAC TGCCTACAGA AGAAACTGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGAGGGAGGA AAGAAACAGG GGCAGCAAAG CCTGGTGTAT CCACGGTACC AAACACAACT 
CAAGCATCAA CTTCTCCGCA GACCCAGACG CCTCAGCAGA ACCCTCCTCC ACCTGTGCAG 
GCCACAACTC ACCCCTTTCC TGCTGTCACC CCAGACCTCA TTGCCCAGCC TCCTGTCATG 
ACAATGGTGC CCCCTCAGCC ACTTCAGACT CCTTCACCGG TACCCCCCCA GCCACCACCC 
CCACCTGCTC CAGTTCCACA GCCTGTGCAG AGTCACCCGC CCATCATTGC GACCACCCCC 
CAGCCTGTGA AGACAAAGAA AGGGGTGAAG AGGAAAGCAG ATACCACCAC CCCTACCACC 
ATCGACCCCA TTCATGAGCC ACCCTCACTG GCCCCAGAGC CCAAGACCGC CAAGCTGGGT 
CCTCGGCGGG AGAGCAGCAG ACCTGTGAAG CCTCCAAAGA AGGATGTACC GGACTCACAG 
CAGCACCCAG GGCCAGAGAA GAGCAGCAAG ATCTCTGAGC AGCTAAAGTG CTGCAGTGGC 
ATCCTCAAGG AGATGTTTGC CAAGAAACAT GCTGCCTATG CCTGGCCTTT CTACAAGCCT 
GTGGATGTGG AGGCACTGGG TCTGCACGAC TACTGTGACA TCATCAAACA TCCCATGGAC 
ATGAGCACAA TCAAGTCTAA ACTAGAGTCC CGAGAGTACA GAGATGCCCA GGAATTTGGT 
GCTGATGTCC GATTGATGTT CTCCAACTGC TACAAGTACA ACCCCCCTGA CCATGAAGTG 
GTAGCCATGG CTCGAAAACT CCAGGATGTG TTTGAAATGC GCTTTGCCAA GATGCCTGAT 
GAGCCTGAAG AGCCAGTTGT TACAGTGTCC TCTCCTGCAG TGCCACCCCC TACAAAGGTG 
GTAGCCCCAC CCTCATCTAG TGACAGCAGC AGCGACAGTT CTTCCGACAG CGACAGTTCC 
ACTGACGACT CTGAGGAAGA GCGAGCCCAG CGGCTGGCTG AACTCCAGGA ACAGCTCAAG 
GCCGTGCATG AGCAGCTTGC AGCCCTCTCA CAGCCCCAGC AGAACAAACC AAAGAAAAAG 
GAGAAGGACA AGAAGGAAAA GAAAAAGGAA AAGCACAAAA AGAAAGAAGA AGTGGAGGAA 
AATAAAAAAA GCAAAACCAA GGAACTTCCT CCCAAAAAGA CAAAGAAAAA TAACAGCAGC 
AACAGCAATG TGAGCAAGAA GGAACCAGTA CCCACGAAGA CCAAGCCGCC TCCCACATAT 
GAATCAGAAG AGGAGGATAA GTGTAAGCCC ATGTCTTATG AGGAGAAGCG GCAGCTAAGT 
CTAGATATCA ACAAACTTCC TGGTGAGAAG CTAGGCCGTG TAGTACACAT AATTCAGTCA 
AGGGAACCAT CACTTAAAAA CTCCAACCCC GATGAGATTG AGATTGACTT TGAGACCCTG 
AAGCCATCTA CACTACGAGA GTTGGAGCGA TATGTCACCT CCTGTTTGCG GAAGAAAAGG 
AAACCTCAAG CTGAAAAAGT TGACGTGATT GCTGGTTCTT CCAAGATGAA GGGATTCTCA 
TCCTCTGAGT CGGAGAGCAC CAGCGAATCC AGCTCCTCTG ACAGTGAAGA CTCTGAAACA 
GAGATGGCTC CCAAGTCAAA AAAGAAGGGG CACACTGGGA GGGACCAGAA AAAGCATCAT 
CACCATCACC ATCCACAGAT GCAGCCAGCC CCAGCTCCTG TGCCCCAGCA GCCGCCCCCA 
CCTCCACAGC AGCCTCCTCC ACCCCCACCT CCGCAGCAGC AGCAGCAGCA ACCTCCACCC 
CCACCACCTC CGCCCTCCAT GCCACAGCAG ACTGCCCCAG CGATGAAGTC CTCGCCCCCA 
CCCTTCATCA CTGCCCAGGT CCCCGTCCTG GAACCACAGC TGCCAGGCAG TGTCTTTGAC 
CCTATTGGCC ACTTCACCCA GCCCATCTTG CACCTGCCGC AGCCGGAGCT GCCTCCTCAC 
CTGCCCCAGC CACCTGAGCA CAGCACTCCA CCCCATCTCA ACCAGCATGC TGTGGTCTCT 
CCTCCAGCTT TGCACAATGC GCTGCCCCAA CAGCCATCTC GGCCCAGTAA CCGAGCTGCT 
GCTCTGCCCC CAAAGCCTAC CCGACCCCCA GCTGTGTCCC CTGCCCTGGC CCAGCCCCCC 
CTGCTCCCAC AACCACCAAT GGCTCAGCCC CCCCAAGTGC TGCTGGAGGA TGAAGAGCCA 
CCTGCCCCAC CCCTCACCTC CATGCAGATG CAGTTGTACC TACAGCAGCT GCAGAAGGTG 
CAGCCTCCCA CACCACTACT CCCTTCCGTG AAGGTGCAGT CCCAACCCCC ACCGCCTTTG 
CCGCCTCCAC CCCACCCTTC TGTGCAGCAG CAGCAGCTCC AGCCACAGCC ACCGCCACCC 
CCACCTCCTC AGCCACAGCC ACCACCCCAG CAACAACACC AGCCTCCACC ACGACCAGTT 
CACTTGCCAT CCATGCCCTT TTCTGCTCAT ATTCAGCAGC CCCCACCACC CCCAGGACAG 
CAACCTACTC ACCCACCCCC AGGACAGCAG CCCCCACCAC CACAGCCTGC CAAGCCCCAG 
CAAGTCATCC AGCATCACCC TTCCCCCCGG CACCACAAGT CAGACCCCTA CTCAGCTGGT 
CATCTTCGTG AGGCTCCCTC TCCGCTTATG ATACATTCCC CTCAGATGCC ACAGTTCCAG 
AGCCTGACCC ATCAGTCTCC TCCCCAGCAA AACGTCCAGC CTAAGAAGCA GGTAAAGGGC 
AGGGCTGAGC CACAGCCACC AGGGCCAGTC ATGGGCCAAG GCCAGGGATG CCCACCTGCC 
TCACCGGCTG CCGTGCCTAT GCTGTCCCAG GAGCTGCGAC CACCTTCAGT CGTCCAGCCC 
CAGCCCCTGG TGGTAGTAAA GGAGGAGAAG ATTCACTCAC CAATCATTCG CAGCGAGCCT 
TTCAGCACCT CACTTCGACC AGAGCCCCCC AAGCACCCAG AGAACATCAA GGCCCCAGTC 
CACCTGCCCC AGCGGCCTGA GATGAAGCCT GTAGACATAG GGAGGCCTGT GATCCGGCCT 
CCAGAGCAGA GTGCACCCCC ACCAGGGGCC CCTGACAAGG ACAAACAGAA GCAGGAGCCA 
AAAACACCAG TGGCACCCAA AAAGGACCTG AAAATTAAGA ACATGGGCTC CTGGGCCAGC 
CTGGTACAGA AGCATCCGAC CACCCCATCC TCCACAGCCA AGTCCTCAAG TGACAGCTTT 
GAGCATTTCC GCCGTGCTGC TCGGGAGAAG GAGGAGAGGG AAAAGGCCCT GAAGGCTCAG 
GCTGAGCATG CAGAGAAGGA GAAGGAGCGG CTGAGGCAGG AGCGCATGAG GAGCCGAGAG 
GATGAAGATG CGCTGGAACA GGCACGTCGA GCCCATGAGG AGGCACGACG GCGCCAGGAG 
CAGCAGCAGC AGCAGCAGCA GCAGAGACAG GAGCAGCAGC AGCAGCAGCA GCAGGCAGCT 
GCTGTGGCCG CTGCCTCTGC CCCCCAGGCC CAGAGCTCCC AGCCCCAGTC TATGCTGGAC 
CAGCAGAGGG AGTTGGCCCG GAAACGAGAG CAGGAGCGGA GGCGCAGGGA GGCAATGGCA 
GCTACAATTG ACATGAATTT CCAGAGTGAT CTTTTGTCAA TATTTGAAGA AAATCTTTTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brd4 ( XM_006524688.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524751.1 Mus musculus bromodomain containing 4 (Brd4), transcript variant X6, mRNA.

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CloneID	OMu16383
Clone ID Related Accession (Same CDS sequence)	XM_030249927.1 , XM_030249925.1 , XM_006524689.2 , NM_020508.4 , XM_006524688.1 , XM_006524688.2 , XM_030249926.1 , XM_006524689.3 , XM_030249924.1
Accession Version	XM_006524688.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4203bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	bromodomain-containing protein 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGCC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGACAGACA 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCCGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAAAAA 
ATCAATGAAC TGCCTACAGA AGAAACTGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGAGGGAGGA AAGAAACAGG GGCAGCAAAG CCTGGTGTAT CCACGGTACC AAACACAACT 
CAAGCATCAA CTTCTCCGCA GACCCAGACG CCTCAGCAGA ACCCTCCTCC ACCTGTGCAG 
GCCACAACTC ACCCCTTTCC TGCTGTCACC CCAGACCTCA TTGCCCAGCC TCCTGTCATG 
ACAATGGTGC CCCCTCAGCC ACTTCAGACT CCTTCACCGG TACCCCCCCA GCCACCACCC 
CCACCTGCTC CAGTTCCACA GCCTGTGCAG AGTCACCCGC CCATCATTGC GACCACCCCC 
CAGCCTGTGA AGACAAAGAA AGGGGTGAAG AGGAAAGCAG ATACCACCAC CCCTACCACC 
ATCGACCCCA TTCATGAGCC ACCCTCACTG GCCCCAGAGC CCAAGACCGC CAAGCTGGGT 
CCTCGGCGGG AGAGCAGCAG ACCTGTGAAG CCTCCAAAGA AGGATGTACC GGACTCACAG 
CAGCACCCAG GGCCAGAGAA GAGCAGCAAG ATCTCTGAGC AGCTAAAGTG CTGCAGTGGC 
ATCCTCAAGG AGATGTTTGC CAAGAAACAT GCTGCCTATG CCTGGCCTTT CTACAAGCCT 
GTGGATGTGG AGGCACTGGG TCTGCACGAC TACTGTGACA TCATCAAACA TCCCATGGAC 
ATGAGCACAA TCAAGTCTAA ACTAGAGTCC CGAGAGTACA GAGATGCCCA GGAATTTGGT 
GCTGATGTCC GATTGATGTT CTCCAACTGC TACAAGTACA ACCCCCCTGA CCATGAAGTG 
GTAGCCATGG CTCGAAAACT CCAGGATGTG TTTGAAATGC GCTTTGCCAA GATGCCTGAT 
GAGCCTGAAG AGCCAGTTGT TACAGTGTCC TCTCCTGCAG TGCCACCCCC TACAAAGGTG 
GTAGCCCCAC CCTCATCTAG TGACAGCAGC AGCGACAGTT CTTCCGACAG CGACAGTTCC 
ACTGACGACT CTGAGGAAGA GCGAGCCCAG CGGCTGGCTG AACTCCAGGA ACAGCTCAAG 
GCCGTGCATG AGCAGCTTGC AGCCCTCTCA CAGCCCCAGC AGAACAAACC AAAGAAAAAG 
GAGAAGGACA AGAAGGAAAA GAAAAAGGAA AAGCACAAAA AGAAAGAAGA AGTGGAGGAA 
AATAAAAAAA GCAAAACCAA GGAACTTCCT CCCAAAAAGA CAAAGAAAAA TAACAGCAGC 
AACAGCAATG TGAGCAAGAA GGAACCAGTA CCCACGAAGA CCAAGCCGCC TCCCACATAT 
GAATCAGAAG AGGAGGATAA GTGTAAGCCC ATGTCTTATG AGGAGAAGCG GCAGCTAAGT 
CTAGATATCA ACAAACTTCC TGGTGAGAAG CTAGGCCGTG TAGTACACAT AATTCAGTCA 
AGGGAACCAT CACTTAAAAA CTCCAACCCC GATGAGATTG AGATTGACTT TGAGACCCTG 
AAGCCATCTA CACTACGAGA GTTGGAGCGA TATGTCACCT CCTGTTTGCG GAAGAAAAGG 
AAACCTCAAG CTGAAAAAGT TGACGTGATT GCTGGTTCTT CCAAGATGAA GGGATTCTCA 
TCCTCTGAGT CGGAGAGCAC CAGCGAATCC AGCTCCTCTG ACAGTGAAGA CTCTGAAACA 
GAGATGGCTC CCAAGTCAAA AAAGAAGGGG CACACTGGGA GGGACCAGAA AAAGCATCAT 
CACCATCACC ATCCACAGAT GCAGCCAGCC CCAGCTCCTG TGCCCCAGCA GCCGCCCCCA 
CCTCCACAGC AGCCTCCTCC ACCCCCACCT CCGCAGCAGC AGCAGCAGCA ACCTCCACCC 
CCACCACCTC CGCCCTCCAT GCCACAGCAG ACTGCCCCAG CGATGAAGTC CTCGCCCCCA 
CCCTTCATCA CTGCCCAGGT CCCCGTCCTG GAACCACAGC TGCCAGGCAG TGTCTTTGAC 
CCTATTGGCC ACTTCACCCA GCCCATCTTG CACCTGCCGC AGCCGGAGCT GCCTCCTCAC 
CTGCCCCAGC CACCTGAGCA CAGCACTCCA CCCCATCTCA ACCAGCATGC TGTGGTCTCT 
CCTCCAGCTT TGCACAATGC GCTGCCCCAA CAGCCATCTC GGCCCAGTAA CCGAGCTGCT 
GCTCTGCCCC CAAAGCCTAC CCGACCCCCA GCTGTGTCCC CTGCCCTGGC CCAGCCCCCC 
CTGCTCCCAC AACCACCAAT GGCTCAGCCC CCCCAAGTGC TGCTGGAGGA TGAAGAGCCA 
CCTGCCCCAC CCCTCACCTC CATGCAGATG CAGTTGTACC TACAGCAGCT GCAGAAGGTG 
CAGCCTCCCA CACCACTACT CCCTTCCGTG AAGGTGCAGT CCCAACCCCC ACCGCCTTTG 
CCGCCTCCAC CCCACCCTTC TGTGCAGCAG CAGCAGCTCC AGCCACAGCC ACCGCCACCC 
CCACCTCCTC AGCCACAGCC ACCACCCCAG CAACAACACC AGCCTCCACC ACGACCAGTT 
CACTTGCCAT CCATGCCCTT TTCTGCTCAT ATTCAGCAGC CCCCACCACC CCCAGGACAG 
CAACCTACTC ACCCACCCCC AGGACAGCAG CCCCCACCAC CACAGCCTGC CAAGCCCCAG 
CAAGTCATCC AGCATCACCC TTCCCCCCGG CACCACAAGT CAGACCCCTA CTCAGCTGGT 
CATCTTCGTG AGGCTCCCTC TCCGCTTATG ATACATTCCC CTCAGATGCC ACAGTTCCAG 
AGCCTGACCC ATCAGTCTCC TCCCCAGCAA AACGTCCAGC CTAAGAAGCA GGTAAAGGGC 
AGGGCTGAGC CACAGCCACC AGGGCCAGTC ATGGGCCAAG GCCAGGGATG CCCACCTGCC 
TCACCGGCTG CCGTGCCTAT GCTGTCCCAG GAGCTGCGAC CACCTTCAGT CGTCCAGCCC 
CAGCCCCTGG TGGTAGTAAA GGAGGAGAAG ATTCACTCAC CAATCATTCG CAGCGAGCCT 
TTCAGCACCT CACTTCGACC AGAGCCCCCC AAGCACCCAG AGAACATCAA GGCCCCAGTC 
CACCTGCCCC AGCGGCCTGA GATGAAGCCT GTAGACATAG GGAGGCCTGT GATCCGGCCT 
CCAGAGCAGA GTGCACCCCC ACCAGGGGCC CCTGACAAGG ACAAACAGAA GCAGGAGCCA 
AAAACACCAG TGGCACCCAA AAAGGACCTG AAAATTAAGA ACATGGGCTC CTGGGCCAGC 
CTGGTACAGA AGCATCCGAC CACCCCATCC TCCACAGCCA AGTCCTCAAG TGACAGCTTT 
GAGCATTTCC GCCGTGCTGC TCGGGAGAAG GAGGAGAGGG AAAAGGCCCT GAAGGCTCAG 
GCTGAGCATG CAGAGAAGGA GAAGGAGCGG CTGAGGCAGG AGCGCATGAG GAGCCGAGAG 
GATGAAGATG CGCTGGAACA GGCACGTCGA GCCCATGAGG AGGCACGACG GCGCCAGGAG 
CAGCAGCAGC AGCAGCAGCA GCAGAGACAG GAGCAGCAGC AGCAGCAGCA GCAGGCAGCT 
GCTGTGGCCG CTGCCTCTGC CCCCCAGGCC CAGAGCTCCC AGCCCCAGTC TATGCTGGAC 
CAGCAGAGGG AGTTGGCCCG GAAACGAGAG CAGGAGCGGA GGCGCAGGGA GGCAATGGCA 
GCTACAATTG ACATGAATTT CCAGAGTGAT CTTTTGTCAA TATTTGAAGA AAATCTTTTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524751.1
CDS	240..4442
Translation	MSTESGPGTRLRNLPVMGDGLETSQMSTTQAQAQPQPANAASTNPPPPETSNPNKPKRQTNQLQYLLRVVLKTLWKHQFAWPFQQPVDAVKLNLPDYYKIIKTPMDMGTIKKRLENNYYWNAQECIQDFNTMFTNCYIYNKPGDDIVLMAEALEKLFLQKINELPTEETEIMIVQAKGRGRGRKETGAAKPGVSTVPNTTQASTSPQTQTPQQNPPPPVQATTHPFPAVTPDLIAQPPVMTMVPPQPLQTPSPVPPQPPPPPAPVPQPVQSHPPIIATTPQPVKTKKGVKRKADTTTPTTIDPIHEPPSLAPEPKTAKLGPRRESSRPVKPPKKDVPDSQQHPGPEKSSKISEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYCDIIKHPMDMSTIKSKLESREYRDAQEFGADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPEEPVVTVSSPAVPPPTKVVAPPSSSDSSSDSSSDSDSSTDDSEEERAQRLAELQEQLKAVHEQLAALSQPQQNKPKKKEKDKKEKKKEKHKKKEEVEENKKSKTKELPPKKTKKNNSSNSNVSKKEPVPTKTKPPPTYESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVDVIAGSSKMKGFSSSESESTSESSSSDSEDSETEMAPKSKKKGHTGRDQKKHHHHHHPQMQPAPAPVPQQPPPPPQQPPPPPPPQQQQQQPPPPPPPPSMPQQTAPAMKSSPPPFITAQVPVLEPQLPGSVFDPIGHFTQPILHLPQPELPPHLPQPPEHSTPPHLNQHAVVSPPALHNALPQQPSRPSNRAAALPPKPTRPPAVSPALAQPPLLPQPPMAQPPQVLLEDEEPPAPPLTSMQMQLYLQQLQKVQPPTPLLPSVKVQSQPPPPLPPPPHPSVQQQQLQPQPPPPPPPQPQPPPQQQHQPPPRPVHLPSMPFSAHIQQPPPPPGQQPTHPPPGQQPPPPQPAKPQQVIQHHPSPRHHKSDPYSAGHLREAPSPLMIHSPQMPQFQSLTHQSPPQQNVQPKKQVKGRAEPQPPGPVMGQGQGCPPASPAAVPMLSQELRPPSVVQPQPLVVVKEEKIHSPIIRSEPFSTSLRPEPPKHPENIKAPVHLPQRPEMKPVDIGRPVIRPPEQSAPPPGAPDKDKQKQEPKTPVAPKKDLKIKNMGSWASLVQKHPTTPSSTAKSSSDSFEHFRRAAREKEEREKALKAQAEHAEKEKERLRQERMRSREDEDALEQARRAHEEARRRQEQQQQQQQQRQEQQQQQQQAAAVAAASAPQAQSSQPQSMLDQQRELARKREQERRRREAMAATIDMNFQSDLLSIFEENLF

Target ORF information:

RefSeq Version	XM_006524688.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus bromodomain containing 4 (Brd4), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524688.1

ORF Insert Sequence:

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGCC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGACAGACA 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCCGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAAAAA 
ATCAATGAAC TGCCTACAGA AGAAACTGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGAGGGAGGA AAGAAACAGG GGCAGCAAAG CCTGGTGTAT CCACGGTACC AAACACAACT 
CAAGCATCAA CTTCTCCGCA GACCCAGACG CCTCAGCAGA ACCCTCCTCC ACCTGTGCAG 
GCCACAACTC ACCCCTTTCC TGCTGTCACC CCAGACCTCA TTGCCCAGCC TCCTGTCATG 
ACAATGGTGC CCCCTCAGCC ACTTCAGACT CCTTCACCGG TACCCCCCCA GCCACCACCC 
CCACCTGCTC CAGTTCCACA GCCTGTGCAG AGTCACCCGC CCATCATTGC GACCACCCCC 
CAGCCTGTGA AGACAAAGAA AGGGGTGAAG AGGAAAGCAG ATACCACCAC CCCTACCACC 
ATCGACCCCA TTCATGAGCC ACCCTCACTG GCCCCAGAGC CCAAGACCGC CAAGCTGGGT 
CCTCGGCGGG AGAGCAGCAG ACCTGTGAAG CCTCCAAAGA AGGATGTACC GGACTCACAG 
CAGCACCCAG GGCCAGAGAA GAGCAGCAAG ATCTCTGAGC AGCTAAAGTG CTGCAGTGGC 
ATCCTCAAGG AGATGTTTGC CAAGAAACAT GCTGCCTATG CCTGGCCTTT CTACAAGCCT 
GTGGATGTGG AGGCACTGGG TCTGCACGAC TACTGTGACA TCATCAAACA TCCCATGGAC 
ATGAGCACAA TCAAGTCTAA ACTAGAGTCC CGAGAGTACA GAGATGCCCA GGAATTTGGT 
GCTGATGTCC GATTGATGTT CTCCAACTGC TACAAGTACA ACCCCCCTGA CCATGAAGTG 
GTAGCCATGG CTCGAAAACT CCAGGATGTG TTTGAAATGC GCTTTGCCAA GATGCCTGAT 
GAGCCTGAAG AGCCAGTTGT TACAGTGTCC TCTCCTGCAG TGCCACCCCC TACAAAGGTG 
GTAGCCCCAC CCTCATCTAG TGACAGCAGC AGCGACAGTT CTTCCGACAG CGACAGTTCC 
ACTGACGACT CTGAGGAAGA GCGAGCCCAG CGGCTGGCTG AACTCCAGGA ACAGCTCAAG 
GCCGTGCATG AGCAGCTTGC AGCCCTCTCA CAGCCCCAGC AGAACAAACC AAAGAAAAAG 
GAGAAGGACA AGAAGGAAAA GAAAAAGGAA AAGCACAAAA AGAAAGAAGA AGTGGAGGAA 
AATAAAAAAA GCAAAACCAA GGAACTTCCT CCCAAAAAGA CAAAGAAAAA TAACAGCAGC 
AACAGCAATG TGAGCAAGAA GGAACCAGTA CCCACGAAGA CCAAGCCGCC TCCCACATAT 
GAATCAGAAG AGGAGGATAA GTGTAAGCCC ATGTCTTATG AGGAGAAGCG GCAGCTAAGT 
CTAGATATCA ACAAACTTCC TGGTGAGAAG CTAGGCCGTG TAGTACACAT AATTCAGTCA 
AGGGAACCAT CACTTAAAAA CTCCAACCCC GATGAGATTG AGATTGACTT TGAGACCCTG 
AAGCCATCTA CACTACGAGA GTTGGAGCGA TATGTCACCT CCTGTTTGCG GAAGAAAAGG 
AAACCTCAAG CTGAAAAAGT TGACGTGATT GCTGGTTCTT CCAAGATGAA GGGATTCTCA 
TCCTCTGAGT CGGAGAGCAC CAGCGAATCC AGCTCCTCTG ACAGTGAAGA CTCTGAAACA 
GAGATGGCTC CCAAGTCAAA AAAGAAGGGG CACACTGGGA GGGACCAGAA AAAGCATCAT 
CACCATCACC ATCCACAGAT GCAGCCAGCC CCAGCTCCTG TGCCCCAGCA GCCGCCCCCA 
CCTCCACAGC AGCCTCCTCC ACCCCCACCT CCGCAGCAGC AGCAGCAGCA ACCTCCACCC 
CCACCACCTC CGCCCTCCAT GCCACAGCAG ACTGCCCCAG CGATGAAGTC CTCGCCCCCA 
CCCTTCATCA CTGCCCAGGT CCCCGTCCTG GAACCACAGC TGCCAGGCAG TGTCTTTGAC 
CCTATTGGCC ACTTCACCCA GCCCATCTTG CACCTGCCGC AGCCGGAGCT GCCTCCTCAC 
CTGCCCCAGC CACCTGAGCA CAGCACTCCA CCCCATCTCA ACCAGCATGC TGTGGTCTCT 
CCTCCAGCTT TGCACAATGC GCTGCCCCAA CAGCCATCTC GGCCCAGTAA CCGAGCTGCT 
GCTCTGCCCC CAAAGCCTAC CCGACCCCCA GCTGTGTCCC CTGCCCTGGC CCAGCCCCCC 
CTGCTCCCAC AACCACCAAT GGCTCAGCCC CCCCAAGTGC TGCTGGAGGA TGAAGAGCCA 
CCTGCCCCAC CCCTCACCTC CATGCAGATG CAGTTGTACC TACAGCAGCT GCAGAAGGTG 
CAGCCTCCCA CACCACTACT CCCTTCCGTG AAGGTGCAGT CCCAACCCCC ACCGCCTTTG 
CCGCCTCCAC CCCACCCTTC TGTGCAGCAG CAGCAGCTCC AGCCACAGCC ACCGCCACCC 
CCACCTCCTC AGCCACAGCC ACCACCCCAG CAACAACACC AGCCTCCACC ACGACCAGTT 
CACTTGCCAT CCATGCCCTT TTCTGCTCAT ATTCAGCAGC CCCCACCACC CCCAGGACAG 
CAACCTACTC ACCCACCCCC AGGACAGCAG CCCCCACCAC CACAGCCTGC CAAGCCCCAG 
CAAGTCATCC AGCATCACCC TTCCCCCCGG CACCACAAGT CAGACCCCTA CTCAGCTGGT 
CATCTTCGTG AGGCTCCCTC TCCGCTTATG ATACATTCCC CTCAGATGCC ACAGTTCCAG 
AGCCTGACCC ATCAGTCTCC TCCCCAGCAA AACGTCCAGC CTAAGAAGCA GGTAAAGGGC 
AGGGCTGAGC CACAGCCACC AGGGCCAGTC ATGGGCCAAG GCCAGGGATG CCCACCTGCC 
TCACCGGCTG CCGTGCCTAT GCTGTCCCAG GAGCTGCGAC CACCTTCAGT CGTCCAGCCC 
CAGCCCCTGG TGGTAGTAAA GGAGGAGAAG ATTCACTCAC CAATCATTCG CAGCGAGCCT 
TTCAGCACCT CACTTCGACC AGAGCCCCCC AAGCACCCAG AGAACATCAA GGCCCCAGTC 
CACCTGCCCC AGCGGCCTGA GATGAAGCCT GTAGACATAG GGAGGCCTGT GATCCGGCCT 
CCAGAGCAGA GTGCACCCCC ACCAGGGGCC CCTGACAAGG ACAAACAGAA GCAGGAGCCA 
AAAACACCAG TGGCACCCAA AAAGGACCTG AAAATTAAGA ACATGGGCTC CTGGGCCAGC 
CTGGTACAGA AGCATCCGAC CACCCCATCC TCCACAGCCA AGTCCTCAAG TGACAGCTTT 
GAGCATTTCC GCCGTGCTGC TCGGGAGAAG GAGGAGAGGG AAAAGGCCCT GAAGGCTCAG 
GCTGAGCATG CAGAGAAGGA GAAGGAGCGG CTGAGGCAGG AGCGCATGAG GAGCCGAGAG 
GATGAAGATG CGCTGGAACA GGCACGTCGA GCCCATGAGG AGGCACGACG GCGCCAGGAG 
CAGCAGCAGC AGCAGCAGCA GCAGAGACAG GAGCAGCAGC AGCAGCAGCA GCAGGCAGCT 
GCTGTGGCCG CTGCCTCTGC CCCCCAGGCC CAGAGCTCCC AGCCCCAGTC TATGCTGGAC 
CAGCAGAGGG AGTTGGCCCG GAAACGAGAG CAGGAGCGGA GGCGCAGGGA GGCAATGGCA 
GCTACAATTG ACATGAATTT CCAGAGTGAT CTTTTGTCAA TATTTGAAGA AAATCTTTTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brd4 ( XM_006524690.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524753.1 Mus musculus bromodomain containing 4 (Brd4), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OMu53863
Clone ID Related Accession (Same CDS sequence)	XM_006524690.2 , XM_006524690.3
Accession Version	XM_006524690.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4107bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	bromodomain-containing protein 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006524690.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGCC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGACAGACA 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCCGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAAAAA 
ATCAATGAAC TGCCTACAGA AGAAACTGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGAGGGAGGA AAGAAACAGG TAGGGCAGCA AAGCCTGGTG TATCCACGGT ACCAAACACA 
ACTCAAGCAT CAACTTCTCC GCAGACCCAG ACGCCTCAGC AGAACCCTCC TCCACCTGTG 
CAGGCCACAA CTCACCCCTT TCCTGCTGTC ACCCCAGACC TCATTGCCCA GCCTCCTGTC 
ATGACAATGG TGCCCCCTCA GCCACTTCAG ACTCCTTCAC CGGTACCCCC CCAGCCACCA 
CCCCCACCTG CTCCAGTTCC ACAGCCTGTG CAGAGTCACC CGCCCATCAT TGCGACCACC 
CCCCAGCCTG TGAAGACAAA GAAAGGGGTG AAGAGGAAAG CAGATACCAC CACCCCTACC 
ACCATCGACC CCATTCATGA GCCACCCTCA CTGGCCCCAG AGCCCAAGAC CGCCAAGCTG 
GGTCCTCGGC GGGAGAGCAG CAGACCTGTG AAGCCTCCAA AGAAGGATGT ACCGGACTCA 
CAGCAGCACC CAGGGCCAGA GAAGAGCAGC AAGATCTCTG AGCAGCTAAA GTGCTGCAGT 
GGCATCCTCA AGGAGATGTT TGCCAAGAAA CATGCTGCCT ATGCCTGGCC TTTCTACAAG 
CCTGTGGATG TGGAGGCACT GGGTCTGCAC GACTACTGTG ACATCATCAA ACATCCCATG 
GACATGAGCA CAATCAAGTC TAAACTAGAG TCCCGAGAGT ACAGAGATGC CCAGGAATTT 
GGTGCTGATG TCCGATTGAT GTTCTCCAAC TGCTACAAGT ACAACCCCCC TGACCATGAA 
GTGGTAGCCA TGGCTCGAAA ACTCCAGGAT GTGTTTGAAA TGCGCTTTGC CAAGATGCCT 
GATGAGCCTG AAGAGCCAGT TGTTACAGTG TCCTCTCCTG CAGTGCCACC CCCTACAAAG 
GTGGTAGCCC CACCCTCATC TAGTGACAGC AGCAGCGACA GTTCTTCCGA CAGCGACAGT 
TCCACTGACG ACTCTGAGGA AGAGCGAGCC CAGCGGCTGG CTGAACTCCA GGAACAGCTC 
AAGGCCGTGC ATGAGCAGCT TGCAGCCCTC TCACAGCCCC AGCAGAACAA ACCAAAGAAA 
AAGGAGAAGG ACAAGAAGGA AAAGAAAAAG GAAAAGCACA AAAAGAAAGA AGAAGTGGAG 
GAAAATAAAA AAAGCAAAAC CAAGGAACTT CCTCCCAAAA AGACAAAGAA AAATAACAGC 
AGCAACAGCA ATGTGAGCAA GAAGGAACCA GTACCCACGA AGACCAAGCC GCCTCCCACA 
TATGAATCAG AAGAGGAGGA TAAGTGTAAG CCCATGTCTT ATGAGGAGAA GCGGCAGCTA 
AGTCTAGATA TCAACAAACT TCCTGGTGAG AAGCTAGGCC GTGTAGTACA CATAATTCAG 
TCAAGGGAAC CATCACTTAA AAACTCCAAC CCCGATGAGA TTGAGATTGA CTTTGAGACC 
CTGAAGCCAT CTACACTACG AGAGTTGGAG CGATATGTCA CCTCCTGTTT GCGGAAGAAA 
AGGAAACCTC AAGCTGAAAA AGTTGACGTG ATTGCTGGTT CTTCCAAGAT GAAGGGATTC 
TCATCCTCTG AGTCGGAGAG CACCAGCGAA TCCAGCTCCT CTGACAGTGA AGACTCTGAA 
ACAGAGATGG CTCCCAAGTC AAAAAAGAAG GGGCACACTG GGAGGGACCA GAAAAAGCAT 
CATCACCATC ACCATCCACA GATGCAGCCA GCCCCAGCTC CTGTGCCCCA GCAGCCGCCC 
CCACCTCCAC AGCAGCCTCC TCCACCCCCA CCTCCGCAGC AGCAGCAGCA GCAACCTCCA 
CCCCCACCAC CTCCGCCCTC CATGCCACAG CAGACTGCCC CAGCGATGAA GTCCTCGCCC 
CCACCCTTCA TCACTGCCCA GGTCCCCGTC CTGGAACCAC AGCTGCCAGG CAGTGTCTTT 
GACCCTATTG GCCACTTCAC CCAGCCCATC TTGCACCTGC CGCAGCCGGA GCTGCCTCCT 
CACCTGCCCC AGCCACCTGA GCACAGCACT CCACCCCATC TCAACCAGCA TGCTGTGGTC 
TCTCCTCCAG CTTTGCACAA TGCGCTGCCC CAACAGCCAT CTCGGCCCAG TAACCGAGCT 
GCTGCTCTGC CCCCAAAGCC TACCCGACCC CCAGCTGTGT CCCCTGCCCT GGCCCAGCCC 
CCCCTGCTCC CACAACCACC AATGGCTCAG CCCCCCCAAG TGCTGCTGGA GGATGAAGAG 
CCACCTGCCC CACCCCTCAC CTCCATGCAG ATGCAGTTGT ACCTACAGCA GCTGCAGAAG 
GTGCAGCCTC CCACACCACT ACTCCCTTCC GTGAAGGTGC AGTCCCAACC CCCACCGCCT 
TTGCCGCCTC CACCCCACCC TTCTGTGCAG CAGCAGCAGC TCCAGCCACA GCCACCGCCA 
CCCCCACCTC CTCAGCCACA GCCACCACCC CAGCAACAAC ACCAGCCTCC ACCACGACCA 
GTTCACTTGC CATCCATGCC CTTTTCTGCT CATATTCAGC AGCCCCCACC ACCCCCAGGA 
CAGCAACCTA CTCACCCACC CCCAGGACAG CAGCCCCCAC CACCACAGCC TGCCAAGCCC 
CAGCAAGTCA TCCAGCATCA CCCTTCCCCC CGGCACCACA AGTCAGACCC CTACTCAGCT 
GGTCATCTTC GTGAGGCTCC CTCTCCGCTT ATGATACATT CCCCTCAGAT GCCACAGTTC 
CAGAGCCTGA CCCATCAGTC TCCTCCCCAG CAAAACGTCC AGCCTAAGAA GCAGGAGCTG 
CGACCACCTT CAGTCGTCCA GCCCCAGCCC CTGGTGGTAG TAAAGGAGGA GAAGATTCAC 
TCACCAATCA TTCGCAGCGA GCCTTTCAGC ACCTCACTTC GACCAGAGCC CCCCAAGCAC 
CCAGAGAACA TCAAGGCCCC AGTCCACCTG CCCCAGCGGC CTGAGATGAA GCCTGTAGAC 
ATAGGGAGGC CTGTGATCCG GCCTCCAGAG CAGAGTGCAC CCCCACCAGG GGCCCCTGAC 
AAGGACAAAC AGAAGCAGGA GCCAAAAACA CCAGTGGCAC CCAAAAAGGA CCTGAAAATT 
AAGAACATGG GCTCCTGGGC CAGCCTGGTA CAGAAGCATC CGACCACCCC ATCCTCCACA 
GCCAAGTCCT CAAGTGACAG CTTTGAGCAT TTCCGCCGTG CTGCTCGGGA GAAGGAGGAG 
AGGGAAAAGG CCCTGAAGGC TCAGGCTGAG CATGCAGAGA AGGAGAAGGA GCGGCTGAGG 
CAGGAGCGCA TGAGGAGCCG AGAGGATGAA GATGCGCTGG AACAGGCACG TCGAGCCCAT 
GAGGAGGCAC GACGGCGCCA GGAGCAGCAG CAGCAGCAGC AGCAGCAGAG ACAGGAGCAG 
CAGCAGCAGC AGCAGCAGGC AGCTGCTGTG GCCGCTGCCT CTGCCCCCCA GGCCCAGAGC 
TCCCAGCCCC AGTCTATGCT GGACCAGCAG AGGGAGTTGG CCCGGAAACG AGAGCAGGAG 
CGGAGGCGCA GGGAGGCAAT GGCAGCTACA ATTGACATGA ATTTCCAGAG TGATCTTTTG 
TCAATATTTG AAGAAAATCT TTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524753.1
CDS	109..4215
Translation	MSTESGPGTRLRNLPVMGDGLETSQMSTTQAQAQPQPANAASTNPPPPETSNPNKPKRQTNQLQYLLRVVLKTLWKHQFAWPFQQPVDAVKLNLPDYYKIIKTPMDMGTIKKRLENNYYWNAQECIQDFNTMFTNCYIYNKPGDDIVLMAEALEKLFLQKINELPTEETEIMIVQAKGRGRGRKETGRAAKPGVSTVPNTTQASTSPQTQTPQQNPPPPVQATTHPFPAVTPDLIAQPPVMTMVPPQPLQTPSPVPPQPPPPPAPVPQPVQSHPPIIATTPQPVKTKKGVKRKADTTTPTTIDPIHEPPSLAPEPKTAKLGPRRESSRPVKPPKKDVPDSQQHPGPEKSSKISEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYCDIIKHPMDMSTIKSKLESREYRDAQEFGADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPEEPVVTVSSPAVPPPTKVVAPPSSSDSSSDSSSDSDSSTDDSEEERAQRLAELQEQLKAVHEQLAALSQPQQNKPKKKEKDKKEKKKEKHKKKEEVEENKKSKTKELPPKKTKKNNSSNSNVSKKEPVPTKTKPPPTYESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVDVIAGSSKMKGFSSSESESTSESSSSDSEDSETEMAPKSKKKGHTGRDQKKHHHHHHPQMQPAPAPVPQQPPPPPQQPPPPPPPQQQQQQPPPPPPPPSMPQQTAPAMKSSPPPFITAQVPVLEPQLPGSVFDPIGHFTQPILHLPQPELPPHLPQPPEHSTPPHLNQHAVVSPPALHNALPQQPSRPSNRAAALPPKPTRPPAVSPALAQPPLLPQPPMAQPPQVLLEDEEPPAPPLTSMQMQLYLQQLQKVQPPTPLLPSVKVQSQPPPPLPPPPHPSVQQQQLQPQPPPPPPPQPQPPPQQQHQPPPRPVHLPSMPFSAHIQQPPPPPGQQPTHPPPGQQPPPPQPAKPQQVIQHHPSPRHHKSDPYSAGHLREAPSPLMIHSPQMPQFQSLTHQSPPQQNVQPKKQELRPPSVVQPQPLVVVKEEKIHSPIIRSEPFSTSLRPEPPKHPENIKAPVHLPQRPEMKPVDIGRPVIRPPEQSAPPPGAPDKDKQKQEPKTPVAPKKDLKIKNMGSWASLVQKHPTTPSSTAKSSSDSFEHFRRAAREKEEREKALKAQAEHAEKEKERLRQERMRSREDEDALEQARRAHEEARRRQEQQQQQQQQRQEQQQQQQQAAAVAAASAPQAQSSQPQSMLDQQRELARKREQERRRREAMAATIDMNFQSDLLSIFEENLF

Target ORF information:

RefSeq Version	XM_006524690.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus bromodomain containing 4 (Brd4), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524690.2

ORF Insert Sequence:

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGCC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGACAGACA 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCCGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAAAAA 
ATCAATGAAC TGCCTACAGA AGAAACTGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGAGGGAGGA AAGAAACAGG TAGGGCAGCA AAGCCTGGTG TATCCACGGT ACCAAACACA 
ACTCAAGCAT CAACTTCTCC GCAGACCCAG ACGCCTCAGC AGAACCCTCC TCCACCTGTG 
CAGGCCACAA CTCACCCCTT TCCTGCTGTC ACCCCAGACC TCATTGCCCA GCCTCCTGTC 
ATGACAATGG TGCCCCCTCA GCCACTTCAG ACTCCTTCAC CGGTACCCCC CCAGCCACCA 
CCCCCACCTG CTCCAGTTCC ACAGCCTGTG CAGAGTCACC CGCCCATCAT TGCGACCACC 
CCCCAGCCTG TGAAGACAAA GAAAGGGGTG AAGAGGAAAG CAGATACCAC CACCCCTACC 
ACCATCGACC CCATTCATGA GCCACCCTCA CTGGCCCCAG AGCCCAAGAC CGCCAAGCTG 
GGTCCTCGGC GGGAGAGCAG CAGACCTGTG AAGCCTCCAA AGAAGGATGT ACCGGACTCA 
CAGCAGCACC CAGGGCCAGA GAAGAGCAGC AAGATCTCTG AGCAGCTAAA GTGCTGCAGT 
GGCATCCTCA AGGAGATGTT TGCCAAGAAA CATGCTGCCT ATGCCTGGCC TTTCTACAAG 
CCTGTGGATG TGGAGGCACT GGGTCTGCAC GACTACTGTG ACATCATCAA ACATCCCATG 
GACATGAGCA CAATCAAGTC TAAACTAGAG TCCCGAGAGT ACAGAGATGC CCAGGAATTT 
GGTGCTGATG TCCGATTGAT GTTCTCCAAC TGCTACAAGT ACAACCCCCC TGACCATGAA 
GTGGTAGCCA TGGCTCGAAA ACTCCAGGAT GTGTTTGAAA TGCGCTTTGC CAAGATGCCT 
GATGAGCCTG AAGAGCCAGT TGTTACAGTG TCCTCTCCTG CAGTGCCACC CCCTACAAAG 
GTGGTAGCCC CACCCTCATC TAGTGACAGC AGCAGCGACA GTTCTTCCGA CAGCGACAGT 
TCCACTGACG ACTCTGAGGA AGAGCGAGCC CAGCGGCTGG CTGAACTCCA GGAACAGCTC 
AAGGCCGTGC ATGAGCAGCT TGCAGCCCTC TCACAGCCCC AGCAGAACAA ACCAAAGAAA 
AAGGAGAAGG ACAAGAAGGA AAAGAAAAAG GAAAAGCACA AAAAGAAAGA AGAAGTGGAG 
GAAAATAAAA AAAGCAAAAC CAAGGAACTT CCTCCCAAAA AGACAAAGAA AAATAACAGC 
AGCAACAGCA ATGTGAGCAA GAAGGAACCA GTACCCACGA AGACCAAGCC GCCTCCCACA 
TATGAATCAG AAGAGGAGGA TAAGTGTAAG CCCATGTCTT ATGAGGAGAA GCGGCAGCTA 
AGTCTAGATA TCAACAAACT TCCTGGTGAG AAGCTAGGCC GTGTAGTACA CATAATTCAG 
TCAAGGGAAC CATCACTTAA AAACTCCAAC CCCGATGAGA TTGAGATTGA CTTTGAGACC 
CTGAAGCCAT CTACACTACG AGAGTTGGAG CGATATGTCA CCTCCTGTTT GCGGAAGAAA 
AGGAAACCTC AAGCTGAAAA AGTTGACGTG ATTGCTGGTT CTTCCAAGAT GAAGGGATTC 
TCATCCTCTG AGTCGGAGAG CACCAGCGAA TCCAGCTCCT CTGACAGTGA AGACTCTGAA 
ACAGAGATGG CTCCCAAGTC AAAAAAGAAG GGGCACACTG GGAGGGACCA GAAAAAGCAT 
CATCACCATC ACCATCCACA GATGCAGCCA GCCCCAGCTC CTGTGCCCCA GCAGCCGCCC 
CCACCTCCAC AGCAGCCTCC TCCACCCCCA CCTCCGCAGC AGCAGCAGCA GCAACCTCCA 
CCCCCACCAC CTCCGCCCTC CATGCCACAG CAGACTGCCC CAGCGATGAA GTCCTCGCCC 
CCACCCTTCA TCACTGCCCA GGTCCCCGTC CTGGAACCAC AGCTGCCAGG CAGTGTCTTT 
GACCCTATTG GCCACTTCAC CCAGCCCATC TTGCACCTGC CGCAGCCGGA GCTGCCTCCT 
CACCTGCCCC AGCCACCTGA GCACAGCACT CCACCCCATC TCAACCAGCA TGCTGTGGTC 
TCTCCTCCAG CTTTGCACAA TGCGCTGCCC CAACAGCCAT CTCGGCCCAG TAACCGAGCT 
GCTGCTCTGC CCCCAAAGCC TACCCGACCC CCAGCTGTGT CCCCTGCCCT GGCCCAGCCC 
CCCCTGCTCC CACAACCACC AATGGCTCAG CCCCCCCAAG TGCTGCTGGA GGATGAAGAG 
CCACCTGCCC CACCCCTCAC CTCCATGCAG ATGCAGTTGT ACCTACAGCA GCTGCAGAAG 
GTGCAGCCTC CCACACCACT ACTCCCTTCC GTGAAGGTGC AGTCCCAACC CCCACCGCCT 
TTGCCGCCTC CACCCCACCC TTCTGTGCAG CAGCAGCAGC TCCAGCCACA GCCACCGCCA 
CCCCCACCTC CTCAGCCACA GCCACCACCC CAGCAACAAC ACCAGCCTCC ACCACGACCA 
GTTCACTTGC CATCCATGCC CTTTTCTGCT CATATTCAGC AGCCCCCACC ACCCCCAGGA 
CAGCAACCTA CTCACCCACC CCCAGGACAG CAGCCCCCAC CACCACAGCC TGCCAAGCCC 
CAGCAAGTCA TCCAGCATCA CCCTTCCCCC CGGCACCACA AGTCAGACCC CTACTCAGCT 
GGTCATCTTC GTGAGGCTCC CTCTCCGCTT ATGATACATT CCCCTCAGAT GCCACAGTTC 
CAGAGCCTGA CCCATCAGTC TCCTCCCCAG CAAAACGTCC AGCCTAAGAA GCAGGAGCTG 
CGACCACCTT CAGTCGTCCA GCCCCAGCCC CTGGTGGTAG TAAAGGAGGA GAAGATTCAC 
TCACCAATCA TTCGCAGCGA GCCTTTCAGC ACCTCACTTC GACCAGAGCC CCCCAAGCAC 
CCAGAGAACA TCAAGGCCCC AGTCCACCTG CCCCAGCGGC CTGAGATGAA GCCTGTAGAC 
ATAGGGAGGC CTGTGATCCG GCCTCCAGAG CAGAGTGCAC CCCCACCAGG GGCCCCTGAC 
AAGGACAAAC AGAAGCAGGA GCCAAAAACA CCAGTGGCAC CCAAAAAGGA CCTGAAAATT 
AAGAACATGG GCTCCTGGGC CAGCCTGGTA CAGAAGCATC CGACCACCCC ATCCTCCACA 
GCCAAGTCCT CAAGTGACAG CTTTGAGCAT TTCCGCCGTG CTGCTCGGGA GAAGGAGGAG 
AGGGAAAAGG CCCTGAAGGC TCAGGCTGAG CATGCAGAGA AGGAGAAGGA GCGGCTGAGG 
CAGGAGCGCA TGAGGAGCCG AGAGGATGAA GATGCGCTGG AACAGGCACG TCGAGCCCAT 
GAGGAGGCAC GACGGCGCCA GGAGCAGCAG CAGCAGCAGC AGCAGCAGAG ACAGGAGCAG 
CAGCAGCAGC AGCAGCAGGC AGCTGCTGTG GCCGCTGCCT CTGCCCCCCA GGCCCAGAGC 
TCCCAGCCCC AGTCTATGCT GGACCAGCAG AGGGAGTTGG CCCGGAAACG AGAGCAGGAG 
CGGAGGCGCA GGGAGGCAAT GGCAGCTACA ATTGACATGA ATTTCCAGAG TGATCTTTTG 
TCAATATTTG AAGAAAATCT TTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brd4 ( XM_006524691.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524754.1 Mus musculus bromodomain containing 4 (Brd4), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OMu53864
Clone ID Related Accession (Same CDS sequence)	XM_030249928.1 , XM_006524691.1 , XM_006524691.2
Accession Version	XM_006524691.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4104bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	bromodomain-containing protein 4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGCC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGACAGACA 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCCGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAAAAA 
ATCAATGAAC TGCCTACAGA AGAAACTGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGAGGGAGGA AAGAAACAGG GGCAGCAAAG CCTGGTGTAT CCACGGTACC AAACACAACT 
CAAGCATCAA CTTCTCCGCA GACCCAGACG CCTCAGCAGA ACCCTCCTCC ACCTGTGCAG 
GCCACAACTC ACCCCTTTCC TGCTGTCACC CCAGACCTCA TTGCCCAGCC TCCTGTCATG 
ACAATGGTGC CCCCTCAGCC ACTTCAGACT CCTTCACCGG TACCCCCCCA GCCACCACCC 
CCACCTGCTC CAGTTCCACA GCCTGTGCAG AGTCACCCGC CCATCATTGC GACCACCCCC 
CAGCCTGTGA AGACAAAGAA AGGGGTGAAG AGGAAAGCAG ATACCACCAC CCCTACCACC 
ATCGACCCCA TTCATGAGCC ACCCTCACTG GCCCCAGAGC CCAAGACCGC CAAGCTGGGT 
CCTCGGCGGG AGAGCAGCAG ACCTGTGAAG CCTCCAAAGA AGGATGTACC GGACTCACAG 
CAGCACCCAG GGCCAGAGAA GAGCAGCAAG ATCTCTGAGC AGCTAAAGTG CTGCAGTGGC 
ATCCTCAAGG AGATGTTTGC CAAGAAACAT GCTGCCTATG CCTGGCCTTT CTACAAGCCT 
GTGGATGTGG AGGCACTGGG TCTGCACGAC TACTGTGACA TCATCAAACA TCCCATGGAC 
ATGAGCACAA TCAAGTCTAA ACTAGAGTCC CGAGAGTACA GAGATGCCCA GGAATTTGGT 
GCTGATGTCC GATTGATGTT CTCCAACTGC TACAAGTACA ACCCCCCTGA CCATGAAGTG 
GTAGCCATGG CTCGAAAACT CCAGGATGTG TTTGAAATGC GCTTTGCCAA GATGCCTGAT 
GAGCCTGAAG AGCCAGTTGT TACAGTGTCC TCTCCTGCAG TGCCACCCCC TACAAAGGTG 
GTAGCCCCAC CCTCATCTAG TGACAGCAGC AGCGACAGTT CTTCCGACAG CGACAGTTCC 
ACTGACGACT CTGAGGAAGA GCGAGCCCAG CGGCTGGCTG AACTCCAGGA ACAGCTCAAG 
GCCGTGCATG AGCAGCTTGC AGCCCTCTCA CAGCCCCAGC AGAACAAACC AAAGAAAAAG 
GAGAAGGACA AGAAGGAAAA GAAAAAGGAA AAGCACAAAA AGAAAGAAGA AGTGGAGGAA 
AATAAAAAAA GCAAAACCAA GGAACTTCCT CCCAAAAAGA CAAAGAAAAA TAACAGCAGC 
AACAGCAATG TGAGCAAGAA GGAACCAGTA CCCACGAAGA CCAAGCCGCC TCCCACATAT 
GAATCAGAAG AGGAGGATAA GTGTAAGCCC ATGTCTTATG AGGAGAAGCG GCAGCTAAGT 
CTAGATATCA ACAAACTTCC TGGTGAGAAG CTAGGCCGTG TAGTACACAT AATTCAGTCA 
AGGGAACCAT CACTTAAAAA CTCCAACCCC GATGAGATTG AGATTGACTT TGAGACCCTG 
AAGCCATCTA CACTACGAGA GTTGGAGCGA TATGTCACCT CCTGTTTGCG GAAGAAAAGG 
AAACCTCAAG CTGAAAAAGT TGACGTGATT GCTGGTTCTT CCAAGATGAA GGGATTCTCA 
TCCTCTGAGT CGGAGAGCAC CAGCGAATCC AGCTCCTCTG ACAGTGAAGA CTCTGAAACA 
GAGATGGCTC CCAAGTCAAA AAAGAAGGGG CACACTGGGA GGGACCAGAA AAAGCATCAT 
CACCATCACC ATCCACAGAT GCAGCCAGCC CCAGCTCCTG TGCCCCAGCA GCCGCCCCCA 
CCTCCACAGC AGCCTCCTCC ACCCCCACCT CCGCAGCAGC AGCAGCAGCA ACCTCCACCC 
CCACCACCTC CGCCCTCCAT GCCACAGCAG ACTGCCCCAG CGATGAAGTC CTCGCCCCCA 
CCCTTCATCA CTGCCCAGGT CCCCGTCCTG GAACCACAGC TGCCAGGCAG TGTCTTTGAC 
CCTATTGGCC ACTTCACCCA GCCCATCTTG CACCTGCCGC AGCCGGAGCT GCCTCCTCAC 
CTGCCCCAGC CACCTGAGCA CAGCACTCCA CCCCATCTCA ACCAGCATGC TGTGGTCTCT 
CCTCCAGCTT TGCACAATGC GCTGCCCCAA CAGCCATCTC GGCCCAGTAA CCGAGCTGCT 
GCTCTGCCCC CAAAGCCTAC CCGACCCCCA GCTGTGTCCC CTGCCCTGGC CCAGCCCCCC 
CTGCTCCCAC AACCACCAAT GGCTCAGCCC CCCCAAGTGC TGCTGGAGGA TGAAGAGCCA 
CCTGCCCCAC CCCTCACCTC CATGCAGATG CAGTTGTACC TACAGCAGCT GCAGAAGGTG 
CAGCCTCCCA CACCACTACT CCCTTCCGTG AAGGTGCAGT CCCAACCCCC ACCGCCTTTG 
CCGCCTCCAC CCCACCCTTC TGTGCAGCAG CAGCAGCTCC AGCCACAGCC ACCGCCACCC 
CCACCTCCTC AGCCACAGCC ACCACCCCAG CAACAACACC AGCCTCCACC ACGACCAGTT 
CACTTGCCAT CCATGCCCTT TTCTGCTCAT ATTCAGCAGC CCCCACCACC CCCAGGACAG 
CAACCTACTC ACCCACCCCC AGGACAGCAG CCCCCACCAC CACAGCCTGC CAAGCCCCAG 
CAAGTCATCC AGCATCACCC TTCCCCCCGG CACCACAAGT CAGACCCCTA CTCAGCTGGT 
CATCTTCGTG AGGCTCCCTC TCCGCTTATG ATACATTCCC CTCAGATGCC ACAGTTCCAG 
AGCCTGACCC ATCAGTCTCC TCCCCAGCAA AACGTCCAGC CTAAGAAGCA GGAGCTGCGA 
CCACCTTCAG TCGTCCAGCC CCAGCCCCTG GTGGTAGTAA AGGAGGAGAA GATTCACTCA 
CCAATCATTC GCAGCGAGCC TTTCAGCACC TCACTTCGAC CAGAGCCCCC CAAGCACCCA 
GAGAACATCA AGGCCCCAGT CCACCTGCCC CAGCGGCCTG AGATGAAGCC TGTAGACATA 
GGGAGGCCTG TGATCCGGCC TCCAGAGCAG AGTGCACCCC CACCAGGGGC CCCTGACAAG 
GACAAACAGA AGCAGGAGCC AAAAACACCA GTGGCACCCA AAAAGGACCT GAAAATTAAG 
AACATGGGCT CCTGGGCCAG CCTGGTACAG AAGCATCCGA CCACCCCATC CTCCACAGCC 
AAGTCCTCAA GTGACAGCTT TGAGCATTTC CGCCGTGCTG CTCGGGAGAA GGAGGAGAGG 
GAAAAGGCCC TGAAGGCTCA GGCTGAGCAT GCAGAGAAGG AGAAGGAGCG GCTGAGGCAG 
GAGCGCATGA GGAGCCGAGA GGATGAAGAT GCGCTGGAAC AGGCACGTCG AGCCCATGAG 
GAGGCACGAC GGCGCCAGGA GCAGCAGCAG CAGCAGCAGC AGCAGAGACA GGAGCAGCAG 
CAGCAGCAGC AGCAGGCAGC TGCTGTGGCC GCTGCCTCTG CCCCCCAGGC CCAGAGCTCC 
CAGCCCCAGT CTATGCTGGA CCAGCAGAGG GAGTTGGCCC GGAAACGAGA GCAGGAGCGG 
AGGCGCAGGG AGGCAATGGC AGCTACAATT GACATGAATT TCCAGAGTGA TCTTTTGTCA 
ATATTTGAAG AAAATCTTTT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524754.1
CDS	240..4343
Translation	MSTESGPGTRLRNLPVMGDGLETSQMSTTQAQAQPQPANAASTNPPPPETSNPNKPKRQTNQLQYLLRVVLKTLWKHQFAWPFQQPVDAVKLNLPDYYKIIKTPMDMGTIKKRLENNYYWNAQECIQDFNTMFTNCYIYNKPGDDIVLMAEALEKLFLQKINELPTEETEIMIVQAKGRGRGRKETGAAKPGVSTVPNTTQASTSPQTQTPQQNPPPPVQATTHPFPAVTPDLIAQPPVMTMVPPQPLQTPSPVPPQPPPPPAPVPQPVQSHPPIIATTPQPVKTKKGVKRKADTTTPTTIDPIHEPPSLAPEPKTAKLGPRRESSRPVKPPKKDVPDSQQHPGPEKSSKISEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYCDIIKHPMDMSTIKSKLESREYRDAQEFGADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPEEPVVTVSSPAVPPPTKVVAPPSSSDSSSDSSSDSDSSTDDSEEERAQRLAELQEQLKAVHEQLAALSQPQQNKPKKKEKDKKEKKKEKHKKKEEVEENKKSKTKELPPKKTKKNNSSNSNVSKKEPVPTKTKPPPTYESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVDVIAGSSKMKGFSSSESESTSESSSSDSEDSETEMAPKSKKKGHTGRDQKKHHHHHHPQMQPAPAPVPQQPPPPPQQPPPPPPPQQQQQQPPPPPPPPSMPQQTAPAMKSSPPPFITAQVPVLEPQLPGSVFDPIGHFTQPILHLPQPELPPHLPQPPEHSTPPHLNQHAVVSPPALHNALPQQPSRPSNRAAALPPKPTRPPAVSPALAQPPLLPQPPMAQPPQVLLEDEEPPAPPLTSMQMQLYLQQLQKVQPPTPLLPSVKVQSQPPPPLPPPPHPSVQQQQLQPQPPPPPPPQPQPPPQQQHQPPPRPVHLPSMPFSAHIQQPPPPPGQQPTHPPPGQQPPPPQPAKPQQVIQHHPSPRHHKSDPYSAGHLREAPSPLMIHSPQMPQFQSLTHQSPPQQNVQPKKQELRPPSVVQPQPLVVVKEEKIHSPIIRSEPFSTSLRPEPPKHPENIKAPVHLPQRPEMKPVDIGRPVIRPPEQSAPPPGAPDKDKQKQEPKTPVAPKKDLKIKNMGSWASLVQKHPTTPSSTAKSSSDSFEHFRRAAREKEEREKALKAQAEHAEKEKERLRQERMRSREDEDALEQARRAHEEARRRQEQQQQQQQQRQEQQQQQQQAAAVAAASAPQAQSSQPQSMLDQQRELARKREQERRRREAMAATIDMNFQSDLLSIFEENLF

Target ORF information:

RefSeq Version	XM_006524691.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus bromodomain containing 4 (Brd4), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524691.1

ORF Insert Sequence:

ATGTCTACGG AGAGCGGCCC TGGGACAAGA TTGAGAAATC TGCCAGTAAT GGGGGATGGA 
CTAGAAACCT CCCAAATGTC TACAACGCAG GCCCAGGCCC AACCCCAGCC AGCAAATGCA 
GCCAGCACCA ATCCTCCACC CCCAGAGACC TCCAACCCTA ACAAGCCCAA GAGACAGACA 
AACCAACTGC AATATCTGCT CAGAGTGGTG CTCAAGACAC TATGGAAACA CCAGTTTGCG 
TGGCCTTTCC AGCAGCCCGT GGATGCCGTC AAGCTGAACC TCCCTGATTA CTATAAGATT 
ATTAAAACAC CCATGGATAT GGGAACAATA AAGAAGCGCT TGGAAAACAA CTATTACTGG 
AATGCTCAGG AATGTATCCA GGACTTCAAC ACTATGTTTA CAAATTGTTA CATCTATAAC 
AAGCCTGGAG ATGACATCGT CTTAATGGCA GAAGCTCTGG AGAAGCTCTT CTTGCAAAAA 
ATCAATGAAC TGCCTACAGA AGAAACTGAG ATCATGATAG TCCAGGCAAA AGGAAGAGGA 
CGAGGGAGGA AAGAAACAGG GGCAGCAAAG CCTGGTGTAT CCACGGTACC AAACACAACT 
CAAGCATCAA CTTCTCCGCA GACCCAGACG CCTCAGCAGA ACCCTCCTCC ACCTGTGCAG 
GCCACAACTC ACCCCTTTCC TGCTGTCACC CCAGACCTCA TTGCCCAGCC TCCTGTCATG 
ACAATGGTGC CCCCTCAGCC ACTTCAGACT CCTTCACCGG TACCCCCCCA GCCACCACCC 
CCACCTGCTC CAGTTCCACA GCCTGTGCAG AGTCACCCGC CCATCATTGC GACCACCCCC 
CAGCCTGTGA AGACAAAGAA AGGGGTGAAG AGGAAAGCAG ATACCACCAC CCCTACCACC 
ATCGACCCCA TTCATGAGCC ACCCTCACTG GCCCCAGAGC CCAAGACCGC CAAGCTGGGT 
CCTCGGCGGG AGAGCAGCAG ACCTGTGAAG CCTCCAAAGA AGGATGTACC GGACTCACAG 
CAGCACCCAG GGCCAGAGAA GAGCAGCAAG ATCTCTGAGC AGCTAAAGTG CTGCAGTGGC 
ATCCTCAAGG AGATGTTTGC CAAGAAACAT GCTGCCTATG CCTGGCCTTT CTACAAGCCT 
GTGGATGTGG AGGCACTGGG TCTGCACGAC TACTGTGACA TCATCAAACA TCCCATGGAC 
ATGAGCACAA TCAAGTCTAA ACTAGAGTCC CGAGAGTACA GAGATGCCCA GGAATTTGGT 
GCTGATGTCC GATTGATGTT CTCCAACTGC TACAAGTACA ACCCCCCTGA CCATGAAGTG 
GTAGCCATGG CTCGAAAACT CCAGGATGTG TTTGAAATGC GCTTTGCCAA GATGCCTGAT 
GAGCCTGAAG AGCCAGTTGT TACAGTGTCC TCTCCTGCAG TGCCACCCCC TACAAAGGTG 
GTAGCCCCAC CCTCATCTAG TGACAGCAGC AGCGACAGTT CTTCCGACAG CGACAGTTCC 
ACTGACGACT CTGAGGAAGA GCGAGCCCAG CGGCTGGCTG AACTCCAGGA ACAGCTCAAG 
GCCGTGCATG AGCAGCTTGC AGCCCTCTCA CAGCCCCAGC AGAACAAACC AAAGAAAAAG 
GAGAAGGACA AGAAGGAAAA GAAAAAGGAA AAGCACAAAA AGAAAGAAGA AGTGGAGGAA 
AATAAAAAAA GCAAAACCAA GGAACTTCCT CCCAAAAAGA CAAAGAAAAA TAACAGCAGC 
AACAGCAATG TGAGCAAGAA GGAACCAGTA CCCACGAAGA CCAAGCCGCC TCCCACATAT 
GAATCAGAAG AGGAGGATAA GTGTAAGCCC ATGTCTTATG AGGAGAAGCG GCAGCTAAGT 
CTAGATATCA ACAAACTTCC TGGTGAGAAG CTAGGCCGTG TAGTACACAT AATTCAGTCA 
AGGGAACCAT CACTTAAAAA CTCCAACCCC GATGAGATTG AGATTGACTT TGAGACCCTG 
AAGCCATCTA CACTACGAGA GTTGGAGCGA TATGTCACCT CCTGTTTGCG GAAGAAAAGG 
AAACCTCAAG CTGAAAAAGT TGACGTGATT GCTGGTTCTT CCAAGATGAA GGGATTCTCA 
TCCTCTGAGT CGGAGAGCAC CAGCGAATCC AGCTCCTCTG ACAGTGAAGA CTCTGAAACA 
GAGATGGCTC CCAAGTCAAA AAAGAAGGGG CACACTGGGA GGGACCAGAA AAAGCATCAT 
CACCATCACC ATCCACAGAT GCAGCCAGCC CCAGCTCCTG TGCCCCAGCA GCCGCCCCCA 
CCTCCACAGC AGCCTCCTCC ACCCCCACCT CCGCAGCAGC AGCAGCAGCA ACCTCCACCC 
CCACCACCTC CGCCCTCCAT GCCACAGCAG ACTGCCCCAG CGATGAAGTC CTCGCCCCCA 
CCCTTCATCA CTGCCCAGGT CCCCGTCCTG GAACCACAGC TGCCAGGCAG TGTCTTTGAC 
CCTATTGGCC ACTTCACCCA GCCCATCTTG CACCTGCCGC AGCCGGAGCT GCCTCCTCAC 
CTGCCCCAGC CACCTGAGCA CAGCACTCCA CCCCATCTCA ACCAGCATGC TGTGGTCTCT 
CCTCCAGCTT TGCACAATGC GCTGCCCCAA CAGCCATCTC GGCCCAGTAA CCGAGCTGCT 
GCTCTGCCCC CAAAGCCTAC CCGACCCCCA GCTGTGTCCC CTGCCCTGGC CCAGCCCCCC 
CTGCTCCCAC AACCACCAAT GGCTCAGCCC CCCCAAGTGC TGCTGGAGGA TGAAGAGCCA 
CCTGCCCCAC CCCTCACCTC CATGCAGATG CAGTTGTACC TACAGCAGCT GCAGAAGGTG 
CAGCCTCCCA CACCACTACT CCCTTCCGTG AAGGTGCAGT CCCAACCCCC ACCGCCTTTG 
CCGCCTCCAC CCCACCCTTC TGTGCAGCAG CAGCAGCTCC AGCCACAGCC ACCGCCACCC 
CCACCTCCTC AGCCACAGCC ACCACCCCAG CAACAACACC AGCCTCCACC ACGACCAGTT 
CACTTGCCAT CCATGCCCTT TTCTGCTCAT ATTCAGCAGC CCCCACCACC CCCAGGACAG 
CAACCTACTC ACCCACCCCC AGGACAGCAG CCCCCACCAC CACAGCCTGC CAAGCCCCAG 
CAAGTCATCC AGCATCACCC TTCCCCCCGG CACCACAAGT CAGACCCCTA CTCAGCTGGT 
CATCTTCGTG AGGCTCCCTC TCCGCTTATG ATACATTCCC CTCAGATGCC ACAGTTCCAG 
AGCCTGACCC ATCAGTCTCC TCCCCAGCAA AACGTCCAGC CTAAGAAGCA GGAGCTGCGA 
CCACCTTCAG TCGTCCAGCC CCAGCCCCTG GTGGTAGTAA AGGAGGAGAA GATTCACTCA 
CCAATCATTC GCAGCGAGCC TTTCAGCACC TCACTTCGAC CAGAGCCCCC CAAGCACCCA 
GAGAACATCA AGGCCCCAGT CCACCTGCCC CAGCGGCCTG AGATGAAGCC TGTAGACATA 
GGGAGGCCTG TGATCCGGCC TCCAGAGCAG AGTGCACCCC CACCAGGGGC CCCTGACAAG 
GACAAACAGA AGCAGGAGCC AAAAACACCA GTGGCACCCA AAAAGGACCT GAAAATTAAG 
AACATGGGCT CCTGGGCCAG CCTGGTACAG AAGCATCCGA CCACCCCATC CTCCACAGCC 
AAGTCCTCAA GTGACAGCTT TGAGCATTTC CGCCGTGCTG CTCGGGAGAA GGAGGAGAGG 
GAAAAGGCCC TGAAGGCTCA GGCTGAGCAT GCAGAGAAGG AGAAGGAGCG GCTGAGGCAG 
GAGCGCATGA GGAGCCGAGA GGATGAAGAT GCGCTGGAAC AGGCACGTCG AGCCCATGAG 
GAGGCACGAC GGCGCCAGGA GCAGCAGCAG CAGCAGCAGC AGCAGAGACA GGAGCAGCAG 
CAGCAGCAGC AGCAGGCAGC TGCTGTGGCC GCTGCCTCTG CCCCCCAGGC CCAGAGCTCC 
CAGCCCCAGT CTATGCTGGA CCAGCAGAGG GAGTTGGCCC GGAAACGAGA GCAGGAGCGG 
AGGCGCAGGG AGGCAATGGC AGCTACAATT GACATGAATT TCCAGAGTGA TCTTTTGTCA 
ATATTTGAAG AAAATCTTTT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brd4 ( XM_011246543.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244845.1 Mus musculus bromodomain containing 4 (Brd4), transcript variant X11, mRNA.

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CloneID	OMu53865
Clone ID Related Accession (Same CDS sequence)	XM_011246543.2 , XM_030249929.1 , XM_011246543.3 , XM_017317588.1 , XM_017317588.2
Accession Version	XM_011246543.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3762bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	bromodomain-containing protein 4 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011246543.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAAG CTCTGGAGAA GCTCTTCTTG CAAAAAATCA ATGAACTGCC TACAGAAGAA 
ACTGAGATCA TGATAGTCCA GGCAAAAGGA AGAGGACGAG GGAGGAAAGA AACAGGTAGG 
GCAGCAAAGC CTGGTGTATC CACGGTACCA AACACAACTC AAGCATCAAC TTCTCCGCAG 
ACCCAGACGC CTCAGCAGAA CCCTCCTCCA CCTGTGCAGG CCACAACTCA CCCCTTTCCT 
GCTGTCACCC CAGACCTCAT TGCCCAGCCT CCTGTCATGA CAATGGTGCC CCCTCAGCCA 
CTTCAGACTC CTTCACCGGT ACCCCCCCAG CCACCACCCC CACCTGCTCC AGTTCCACAG 
CCTGTGCAGA GTCACCCGCC CATCATTGCG ACCACCCCCC AGCCTGTGAA GACAAAGAAA 
GGGGTGAAGA GGAAAGCAGA TACCACCACC CCTACCACCA TCGACCCCAT TCATGAGCCA 
CCCTCACTGG CCCCAGAGCC CAAGACCGCC AAGCTGGGTC CTCGGCGGGA GAGCAGCAGA 
CCTGTGAAGC CTCCAAAGAA GGATGTACCG GACTCACAGC AGCACCCAGG GCCAGAGAAG 
AGCAGCAAGA TCTCTGAGCA GCTAAAGTGC TGCAGTGGCA TCCTCAAGGA GATGTTTGCC 
AAGAAACATG CTGCCTATGC CTGGCCTTTC TACAAGCCTG TGGATGTGGA GGCACTGGGT 
CTGCACGACT ACTGTGACAT CATCAAACAT CCCATGGACA TGAGCACAAT CAAGTCTAAA 
CTAGAGTCCC GAGAGTACAG AGATGCCCAG GAATTTGGTG CTGATGTCCG ATTGATGTTC 
TCCAACTGCT ACAAGTACAA CCCCCCTGAC CATGAAGTGG TAGCCATGGC TCGAAAACTC 
CAGGATGTGT TTGAAATGCG CTTTGCCAAG ATGCCTGATG AGCCTGAAGA GCCAGTTGTT 
ACAGTGTCCT CTCCTGCAGT GCCACCCCCT ACAAAGGTGG TAGCCCCACC CTCATCTAGT 
GACAGCAGCA GCGACAGTTC TTCCGACAGC GACAGTTCCA CTGACGACTC TGAGGAAGAG 
CGAGCCCAGC GGCTGGCTGA ACTCCAGGAA CAGCTCAAGG CCGTGCATGA GCAGCTTGCA 
GCCCTCTCAC AGCCCCAGCA GAACAAACCA AAGAAAAAGG AGAAGGACAA GAAGGAAAAG 
AAAAAGGAAA AGCACAAAAA GAAAGAAGAA GTGGAGGAAA ATAAAAAAAG CAAAACCAAG 
GAACTTCCTC CCAAAAAGAC AAAGAAAAAT AACAGCAGCA ACAGCAATGT GAGCAAGAAG 
GAACCAGTAC CCACGAAGAC CAAGCCGCCT CCCACATATG AATCAGAAGA GGAGGATAAG 
TGTAAGCCCA TGTCTTATGA GGAGAAGCGG CAGCTAAGTC TAGATATCAA CAAACTTCCT 
GGTGAGAAGC TAGGCCGTGT AGTACACATA ATTCAGTCAA GGGAACCATC ACTTAAAAAC 
TCCAACCCCG ATGAGATTGA GATTGACTTT GAGACCCTGA AGCCATCTAC ACTACGAGAG 
TTGGAGCGAT ATGTCACCTC CTGTTTGCGG AAGAAAAGGA AACCTCAAGC TGAAAAAGTT 
GACGTGATTG CTGGTTCTTC CAAGATGAAG GGATTCTCAT CCTCTGAGTC GGAGAGCACC 
AGCGAATCCA GCTCCTCTGA CAGTGAAGAC TCTGAAACAG AGATGGCTCC CAAGTCAAAA 
AAGAAGGGGC ACACTGGGAG GGACCAGAAA AAGCATCATC ACCATCACCA TCCACAGATG 
CAGCCAGCCC CAGCTCCTGT GCCCCAGCAG CCGCCCCCAC CTCCACAGCA GCCTCCTCCA 
CCCCCACCTC CGCAGCAGCA GCAGCAGCAA CCTCCACCCC CACCACCTCC GCCCTCCATG 
CCACAGCAGA CTGCCCCAGC GATGAAGTCC TCGCCCCCAC CCTTCATCAC TGCCCAGGTC 
CCCGTCCTGG AACCACAGCT GCCAGGCAGT GTCTTTGACC CTATTGGCCA CTTCACCCAG 
CCCATCTTGC ACCTGCCGCA GCCGGAGCTG CCTCCTCACC TGCCCCAGCC ACCTGAGCAC 
AGCACTCCAC CCCATCTCAA CCAGCATGCT GTGGTCTCTC CTCCAGCTTT GCACAATGCG 
CTGCCCCAAC AGCCATCTCG GCCCAGTAAC CGAGCTGCTG CTCTGCCCCC AAAGCCTACC 
CGACCCCCAG CTGTGTCCCC TGCCCTGGCC CAGCCCCCCC TGCTCCCACA ACCACCAATG 
GCTCAGCCCC CCCAAGTGCT GCTGGAGGAT GAAGAGCCAC CTGCCCCACC CCTCACCTCC 
ATGCAGATGC AGTTGTACCT ACAGCAGCTG CAGAAGGTGC AGCCTCCCAC ACCACTACTC 
CCTTCCGTGA AGGTGCAGTC CCAACCCCCA CCGCCTTTGC CGCCTCCACC CCACCCTTCT 
GTGCAGCAGC AGCAGCTCCA GCCACAGCCA CCGCCACCCC CACCTCCTCA GCCACAGCCA 
CCACCCCAGC AACAACACCA GCCTCCACCA CGACCAGTTC ACTTGCCATC CATGCCCTTT 
TCTGCTCATA TTCAGCAGCC CCCACCACCC CCAGGACAGC AACCTACTCA CCCACCCCCA 
GGACAGCAGC CCCCACCACC ACAGCCTGCC AAGCCCCAGC AAGTCATCCA GCATCACCCT 
TCCCCCCGGC ACCACAAGTC AGACCCCTAC TCAGCTGGTC ATCTTCGTGA GGCTCCCTCT 
CCGCTTATGA TACATTCCCC TCAGATGCCA CAGTTCCAGA GCCTGACCCA TCAGTCTCCT 
CCCCAGCAAA ACGTCCAGCC TAAGAAGCAG GTAAAGGGCA GGGCTGAGCC ACAGCCACCA 
GGGCCAGTCA TGGGCCAAGG CCAGGGATGC CCACCTGCCT CACCGGCTGC CGTGCCTATG 
CTGTCCCAGG AGCTGCGACC ACCTTCAGTC GTCCAGCCCC AGCCCCTGGT GGTAGTAAAG 
GAGGAGAAGA TTCACTCACC AATCATTCGC AGCGAGCCTT TCAGCACCTC ACTTCGACCA 
GAGCCCCCCA AGCACCCAGA GAACATCAAG GCCCCAGTCC ACCTGCCCCA GCGGCCTGAG 
ATGAAGCCTG TAGACATAGG GAGGCCTGTG ATCCGGCCTC CAGAGCAGAG TGCACCCCCA 
CCAGGGGCCC CTGACAAGGA CAAACAGAAG CAGGAGCCAA AAACACCAGT GGCACCCAAA 
AAGGACCTGA AAATTAAGAA CATGGGCTCC TGGGCCAGCC TGGTACAGAA GCATCCGACC 
ACCCCATCCT CCACAGCCAA GTCCTCAAGT GACAGCTTTG AGCATTTCCG CCGTGCTGCT 
CGGGAGAAGG AGGAGAGGGA AAAGGCCCTG AAGGCTCAGG CTGAGCATGC AGAGAAGGAG 
AAGGAGCGGC TGAGGCAGGA GCGCATGAGG AGCCGAGAGG ATGAAGATGC GCTGGAACAG 
GCACGTCGAG CCCATGAGGA GGCACGACGG CGCCAGGAGC AGCAGCAGCA GCAGCAGCAG 
CAGAGACAGG AGCAGCAGCA GCAGCAGCAG CAGGCAGCTG CTGTGGCCGC TGCCTCTGCC 
CCCCAGGCCC AGAGCTCCCA GCCCCAGTCT ATGCTGGACC AGCAGAGGGA GTTGGCCCGG 
AAACGAGAGC AGGAGCGGAG GCGCAGGGAG GCAATGGCAG CTACAATTGA CATGAATTTC 
CAGAGTGATC TTTTGTCAAT ATTTGAAGAA AATCTTTTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244845.1
CDS	223..3984
Translation	MAEALEKLFLQKINELPTEETEIMIVQAKGRGRGRKETGRAAKPGVSTVPNTTQASTSPQTQTPQQNPPPPVQATTHPFPAVTPDLIAQPPVMTMVPPQPLQTPSPVPPQPPPPPAPVPQPVQSHPPIIATTPQPVKTKKGVKRKADTTTPTTIDPIHEPPSLAPEPKTAKLGPRRESSRPVKPPKKDVPDSQQHPGPEKSSKISEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYCDIIKHPMDMSTIKSKLESREYRDAQEFGADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPEEPVVTVSSPAVPPPTKVVAPPSSSDSSSDSSSDSDSSTDDSEEERAQRLAELQEQLKAVHEQLAALSQPQQNKPKKKEKDKKEKKKEKHKKKEEVEENKKSKTKELPPKKTKKNNSSNSNVSKKEPVPTKTKPPPTYESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVDVIAGSSKMKGFSSSESESTSESSSSDSEDSETEMAPKSKKKGHTGRDQKKHHHHHHPQMQPAPAPVPQQPPPPPQQPPPPPPPQQQQQQPPPPPPPPSMPQQTAPAMKSSPPPFITAQVPVLEPQLPGSVFDPIGHFTQPILHLPQPELPPHLPQPPEHSTPPHLNQHAVVSPPALHNALPQQPSRPSNRAAALPPKPTRPPAVSPALAQPPLLPQPPMAQPPQVLLEDEEPPAPPLTSMQMQLYLQQLQKVQPPTPLLPSVKVQSQPPPPLPPPPHPSVQQQQLQPQPPPPPPPQPQPPPQQQHQPPPRPVHLPSMPFSAHIQQPPPPPGQQPTHPPPGQQPPPPQPAKPQQVIQHHPSPRHHKSDPYSAGHLREAPSPLMIHSPQMPQFQSLTHQSPPQQNVQPKKQVKGRAEPQPPGPVMGQGQGCPPASPAAVPMLSQELRPPSVVQPQPLVVVKEEKIHSPIIRSEPFSTSLRPEPPKHPENIKAPVHLPQRPEMKPVDIGRPVIRPPEQSAPPPGAPDKDKQKQEPKTPVAPKKDLKIKNMGSWASLVQKHPTTPSSTAKSSSDSFEHFRRAAREKEEREKALKAQAEHAEKEKERLRQERMRSREDEDALEQARRAHEEARRRQEQQQQQQQQRQEQQQQQQQAAAVAAASAPQAQSSQPQSMLDQQRELARKREQERRRREAMAATIDMNFQSDLLSIFEENLF

Target ORF information:

RefSeq Version	XM_011246543.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus bromodomain containing 4 (Brd4), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011246543.2

ORF Insert Sequence:

ATGGCAGAAG CTCTGGAGAA GCTCTTCTTG CAAAAAATCA ATGAACTGCC TACAGAAGAA 
ACTGAGATCA TGATAGTCCA GGCAAAAGGA AGAGGACGAG GGAGGAAAGA AACAGGTAGG 
GCAGCAAAGC CTGGTGTATC CACGGTACCA AACACAACTC AAGCATCAAC TTCTCCGCAG 
ACCCAGACGC CTCAGCAGAA CCCTCCTCCA CCTGTGCAGG CCACAACTCA CCCCTTTCCT 
GCTGTCACCC CAGACCTCAT TGCCCAGCCT CCTGTCATGA CAATGGTGCC CCCTCAGCCA 
CTTCAGACTC CTTCACCGGT ACCCCCCCAG CCACCACCCC CACCTGCTCC AGTTCCACAG 
CCTGTGCAGA GTCACCCGCC CATCATTGCG ACCACCCCCC AGCCTGTGAA GACAAAGAAA 
GGGGTGAAGA GGAAAGCAGA TACCACCACC CCTACCACCA TCGACCCCAT TCATGAGCCA 
CCCTCACTGG CCCCAGAGCC CAAGACCGCC AAGCTGGGTC CTCGGCGGGA GAGCAGCAGA 
CCTGTGAAGC CTCCAAAGAA GGATGTACCG GACTCACAGC AGCACCCAGG GCCAGAGAAG 
AGCAGCAAGA TCTCTGAGCA GCTAAAGTGC TGCAGTGGCA TCCTCAAGGA GATGTTTGCC 
AAGAAACATG CTGCCTATGC CTGGCCTTTC TACAAGCCTG TGGATGTGGA GGCACTGGGT 
CTGCACGACT ACTGTGACAT CATCAAACAT CCCATGGACA TGAGCACAAT CAAGTCTAAA 
CTAGAGTCCC GAGAGTACAG AGATGCCCAG GAATTTGGTG CTGATGTCCG ATTGATGTTC 
TCCAACTGCT ACAAGTACAA CCCCCCTGAC CATGAAGTGG TAGCCATGGC TCGAAAACTC 
CAGGATGTGT TTGAAATGCG CTTTGCCAAG ATGCCTGATG AGCCTGAAGA GCCAGTTGTT 
ACAGTGTCCT CTCCTGCAGT GCCACCCCCT ACAAAGGTGG TAGCCCCACC CTCATCTAGT 
GACAGCAGCA GCGACAGTTC TTCCGACAGC GACAGTTCCA CTGACGACTC TGAGGAAGAG 
CGAGCCCAGC GGCTGGCTGA ACTCCAGGAA CAGCTCAAGG CCGTGCATGA GCAGCTTGCA 
GCCCTCTCAC AGCCCCAGCA GAACAAACCA AAGAAAAAGG AGAAGGACAA GAAGGAAAAG 
AAAAAGGAAA AGCACAAAAA GAAAGAAGAA GTGGAGGAAA ATAAAAAAAG CAAAACCAAG 
GAACTTCCTC CCAAAAAGAC AAAGAAAAAT AACAGCAGCA ACAGCAATGT GAGCAAGAAG 
GAACCAGTAC CCACGAAGAC CAAGCCGCCT CCCACATATG AATCAGAAGA GGAGGATAAG 
TGTAAGCCCA TGTCTTATGA GGAGAAGCGG CAGCTAAGTC TAGATATCAA CAAACTTCCT 
GGTGAGAAGC TAGGCCGTGT AGTACACATA ATTCAGTCAA GGGAACCATC ACTTAAAAAC 
TCCAACCCCG ATGAGATTGA GATTGACTTT GAGACCCTGA AGCCATCTAC ACTACGAGAG 
TTGGAGCGAT ATGTCACCTC CTGTTTGCGG AAGAAAAGGA AACCTCAAGC TGAAAAAGTT 
GACGTGATTG CTGGTTCTTC CAAGATGAAG GGATTCTCAT CCTCTGAGTC GGAGAGCACC 
AGCGAATCCA GCTCCTCTGA CAGTGAAGAC TCTGAAACAG AGATGGCTCC CAAGTCAAAA 
AAGAAGGGGC ACACTGGGAG GGACCAGAAA AAGCATCATC ACCATCACCA TCCACAGATG 
CAGCCAGCCC CAGCTCCTGT GCCCCAGCAG CCGCCCCCAC CTCCACAGCA GCCTCCTCCA 
CCCCCACCTC CGCAGCAGCA GCAGCAGCAA CCTCCACCCC CACCACCTCC GCCCTCCATG 
CCACAGCAGA CTGCCCCAGC GATGAAGTCC TCGCCCCCAC CCTTCATCAC TGCCCAGGTC 
CCCGTCCTGG AACCACAGCT GCCAGGCAGT GTCTTTGACC CTATTGGCCA CTTCACCCAG 
CCCATCTTGC ACCTGCCGCA GCCGGAGCTG CCTCCTCACC TGCCCCAGCC ACCTGAGCAC 
AGCACTCCAC CCCATCTCAA CCAGCATGCT GTGGTCTCTC CTCCAGCTTT GCACAATGCG 
CTGCCCCAAC AGCCATCTCG GCCCAGTAAC CGAGCTGCTG CTCTGCCCCC AAAGCCTACC 
CGACCCCCAG CTGTGTCCCC TGCCCTGGCC CAGCCCCCCC TGCTCCCACA ACCACCAATG 
GCTCAGCCCC CCCAAGTGCT GCTGGAGGAT GAAGAGCCAC CTGCCCCACC CCTCACCTCC 
ATGCAGATGC AGTTGTACCT ACAGCAGCTG CAGAAGGTGC AGCCTCCCAC ACCACTACTC 
CCTTCCGTGA AGGTGCAGTC CCAACCCCCA CCGCCTTTGC CGCCTCCACC CCACCCTTCT 
GTGCAGCAGC AGCAGCTCCA GCCACAGCCA CCGCCACCCC CACCTCCTCA GCCACAGCCA 
CCACCCCAGC AACAACACCA GCCTCCACCA CGACCAGTTC ACTTGCCATC CATGCCCTTT 
TCTGCTCATA TTCAGCAGCC CCCACCACCC CCAGGACAGC AACCTACTCA CCCACCCCCA 
GGACAGCAGC CCCCACCACC ACAGCCTGCC AAGCCCCAGC AAGTCATCCA GCATCACCCT 
TCCCCCCGGC ACCACAAGTC AGACCCCTAC TCAGCTGGTC ATCTTCGTGA GGCTCCCTCT 
CCGCTTATGA TACATTCCCC TCAGATGCCA CAGTTCCAGA GCCTGACCCA TCAGTCTCCT 
CCCCAGCAAA ACGTCCAGCC TAAGAAGCAG GTAAAGGGCA GGGCTGAGCC ACAGCCACCA 
GGGCCAGTCA TGGGCCAAGG CCAGGGATGC CCACCTGCCT CACCGGCTGC CGTGCCTATG 
CTGTCCCAGG AGCTGCGACC ACCTTCAGTC GTCCAGCCCC AGCCCCTGGT GGTAGTAAAG 
GAGGAGAAGA TTCACTCACC AATCATTCGC AGCGAGCCTT TCAGCACCTC ACTTCGACCA 
GAGCCCCCCA AGCACCCAGA GAACATCAAG GCCCCAGTCC ACCTGCCCCA GCGGCCTGAG 
ATGAAGCCTG TAGACATAGG GAGGCCTGTG ATCCGGCCTC CAGAGCAGAG TGCACCCCCA 
CCAGGGGCCC CTGACAAGGA CAAACAGAAG CAGGAGCCAA AAACACCAGT GGCACCCAAA 
AAGGACCTGA AAATTAAGAA CATGGGCTCC TGGGCCAGCC TGGTACAGAA GCATCCGACC 
ACCCCATCCT CCACAGCCAA GTCCTCAAGT GACAGCTTTG AGCATTTCCG CCGTGCTGCT 
CGGGAGAAGG AGGAGAGGGA AAAGGCCCTG AAGGCTCAGG CTGAGCATGC AGAGAAGGAG 
AAGGAGCGGC TGAGGCAGGA GCGCATGAGG AGCCGAGAGG ATGAAGATGC GCTGGAACAG 
GCACGTCGAG CCCATGAGGA GGCACGACGG CGCCAGGAGC AGCAGCAGCA GCAGCAGCAG 
CAGAGACAGG AGCAGCAGCA GCAGCAGCAG CAGGCAGCTG CTGTGGCCGC TGCCTCTGCC 
CCCCAGGCCC AGAGCTCCCA GCCCCAGTCT ATGCTGGACC AGCAGAGGGA GTTGGCCCGG 
AAACGAGAGC AGGAGCGGAG GCGCAGGGAG GCAATGGCAG CTACAATTGA CATGAATTTC 
CAGAGTGATC TTTTGTCAAT ATTTGAAGAA AATCTTTTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brd4 ( XM_017317588.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017173077.1 Mus musculus bromodomain containing 4 (Brd4), transcript variant X10, mRNA.

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CloneID	OMu53865
Clone ID Related Accession (Same CDS sequence)	XM_011246543.2 , XM_030249929.1 , XM_011246543.3 , XM_017317588.1 , XM_017317588.2
Accession Version	XM_017317588.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3762bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	bromodomain-containing protein 4 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAAG CTCTGGAGAA GCTCTTCTTG CAAAAAATCA ATGAACTGCC TACAGAAGAA 
ACTGAGATCA TGATAGTCCA GGCAAAAGGA AGAGGACGAG GGAGGAAAGA AACAGGTAGG 
GCAGCAAAGC CTGGTGTATC CACGGTACCA AACACAACTC AAGCATCAAC TTCTCCGCAG 
ACCCAGACGC CTCAGCAGAA CCCTCCTCCA CCTGTGCAGG CCACAACTCA CCCCTTTCCT 
GCTGTCACCC CAGACCTCAT TGCCCAGCCT CCTGTCATGA CAATGGTGCC CCCTCAGCCA 
CTTCAGACTC CTTCACCGGT ACCCCCCCAG CCACCACCCC CACCTGCTCC AGTTCCACAG 
CCTGTGCAGA GTCACCCGCC CATCATTGCG ACCACCCCCC AGCCTGTGAA GACAAAGAAA 
GGGGTGAAGA GGAAAGCAGA TACCACCACC CCTACCACCA TCGACCCCAT TCATGAGCCA 
CCCTCACTGG CCCCAGAGCC CAAGACCGCC AAGCTGGGTC CTCGGCGGGA GAGCAGCAGA 
CCTGTGAAGC CTCCAAAGAA GGATGTACCG GACTCACAGC AGCACCCAGG GCCAGAGAAG 
AGCAGCAAGA TCTCTGAGCA GCTAAAGTGC TGCAGTGGCA TCCTCAAGGA GATGTTTGCC 
AAGAAACATG CTGCCTATGC CTGGCCTTTC TACAAGCCTG TGGATGTGGA GGCACTGGGT 
CTGCACGACT ACTGTGACAT CATCAAACAT CCCATGGACA TGAGCACAAT CAAGTCTAAA 
CTAGAGTCCC GAGAGTACAG AGATGCCCAG GAATTTGGTG CTGATGTCCG ATTGATGTTC 
TCCAACTGCT ACAAGTACAA CCCCCCTGAC CATGAAGTGG TAGCCATGGC TCGAAAACTC 
CAGGATGTGT TTGAAATGCG CTTTGCCAAG ATGCCTGATG AGCCTGAAGA GCCAGTTGTT 
ACAGTGTCCT CTCCTGCAGT GCCACCCCCT ACAAAGGTGG TAGCCCCACC CTCATCTAGT 
GACAGCAGCA GCGACAGTTC TTCCGACAGC GACAGTTCCA CTGACGACTC TGAGGAAGAG 
CGAGCCCAGC GGCTGGCTGA ACTCCAGGAA CAGCTCAAGG CCGTGCATGA GCAGCTTGCA 
GCCCTCTCAC AGCCCCAGCA GAACAAACCA AAGAAAAAGG AGAAGGACAA GAAGGAAAAG 
AAAAAGGAAA AGCACAAAAA GAAAGAAGAA GTGGAGGAAA ATAAAAAAAG CAAAACCAAG 
GAACTTCCTC CCAAAAAGAC AAAGAAAAAT AACAGCAGCA ACAGCAATGT GAGCAAGAAG 
GAACCAGTAC CCACGAAGAC CAAGCCGCCT CCCACATATG AATCAGAAGA GGAGGATAAG 
TGTAAGCCCA TGTCTTATGA GGAGAAGCGG CAGCTAAGTC TAGATATCAA CAAACTTCCT 
GGTGAGAAGC TAGGCCGTGT AGTACACATA ATTCAGTCAA GGGAACCATC ACTTAAAAAC 
TCCAACCCCG ATGAGATTGA GATTGACTTT GAGACCCTGA AGCCATCTAC ACTACGAGAG 
TTGGAGCGAT ATGTCACCTC CTGTTTGCGG AAGAAAAGGA AACCTCAAGC TGAAAAAGTT 
GACGTGATTG CTGGTTCTTC CAAGATGAAG GGATTCTCAT CCTCTGAGTC GGAGAGCACC 
AGCGAATCCA GCTCCTCTGA CAGTGAAGAC TCTGAAACAG AGATGGCTCC CAAGTCAAAA 
AAGAAGGGGC ACACTGGGAG GGACCAGAAA AAGCATCATC ACCATCACCA TCCACAGATG 
CAGCCAGCCC CAGCTCCTGT GCCCCAGCAG CCGCCCCCAC CTCCACAGCA GCCTCCTCCA 
CCCCCACCTC CGCAGCAGCA GCAGCAGCAA CCTCCACCCC CACCACCTCC GCCCTCCATG 
CCACAGCAGA CTGCCCCAGC GATGAAGTCC TCGCCCCCAC CCTTCATCAC TGCCCAGGTC 
CCCGTCCTGG AACCACAGCT GCCAGGCAGT GTCTTTGACC CTATTGGCCA CTTCACCCAG 
CCCATCTTGC ACCTGCCGCA GCCGGAGCTG CCTCCTCACC TGCCCCAGCC ACCTGAGCAC 
AGCACTCCAC CCCATCTCAA CCAGCATGCT GTGGTCTCTC CTCCAGCTTT GCACAATGCG 
CTGCCCCAAC AGCCATCTCG GCCCAGTAAC CGAGCTGCTG CTCTGCCCCC AAAGCCTACC 
CGACCCCCAG CTGTGTCCCC TGCCCTGGCC CAGCCCCCCC TGCTCCCACA ACCACCAATG 
GCTCAGCCCC CCCAAGTGCT GCTGGAGGAT GAAGAGCCAC CTGCCCCACC CCTCACCTCC 
ATGCAGATGC AGTTGTACCT ACAGCAGCTG CAGAAGGTGC AGCCTCCCAC ACCACTACTC 
CCTTCCGTGA AGGTGCAGTC CCAACCCCCA CCGCCTTTGC CGCCTCCACC CCACCCTTCT 
GTGCAGCAGC AGCAGCTCCA GCCACAGCCA CCGCCACCCC CACCTCCTCA GCCACAGCCA 
CCACCCCAGC AACAACACCA GCCTCCACCA CGACCAGTTC ACTTGCCATC CATGCCCTTT 
TCTGCTCATA TTCAGCAGCC CCCACCACCC CCAGGACAGC AACCTACTCA CCCACCCCCA 
GGACAGCAGC CCCCACCACC ACAGCCTGCC AAGCCCCAGC AAGTCATCCA GCATCACCCT 
TCCCCCCGGC ACCACAAGTC AGACCCCTAC TCAGCTGGTC ATCTTCGTGA GGCTCCCTCT 
CCGCTTATGA TACATTCCCC TCAGATGCCA CAGTTCCAGA GCCTGACCCA TCAGTCTCCT 
CCCCAGCAAA ACGTCCAGCC TAAGAAGCAG GTAAAGGGCA GGGCTGAGCC ACAGCCACCA 
GGGCCAGTCA TGGGCCAAGG CCAGGGATGC CCACCTGCCT CACCGGCTGC CGTGCCTATG 
CTGTCCCAGG AGCTGCGACC ACCTTCAGTC GTCCAGCCCC AGCCCCTGGT GGTAGTAAAG 
GAGGAGAAGA TTCACTCACC AATCATTCGC AGCGAGCCTT TCAGCACCTC ACTTCGACCA 
GAGCCCCCCA AGCACCCAGA GAACATCAAG GCCCCAGTCC ACCTGCCCCA GCGGCCTGAG 
ATGAAGCCTG TAGACATAGG GAGGCCTGTG ATCCGGCCTC CAGAGCAGAG TGCACCCCCA 
CCAGGGGCCC CTGACAAGGA CAAACAGAAG CAGGAGCCAA AAACACCAGT GGCACCCAAA 
AAGGACCTGA AAATTAAGAA CATGGGCTCC TGGGCCAGCC TGGTACAGAA GCATCCGACC 
ACCCCATCCT CCACAGCCAA GTCCTCAAGT GACAGCTTTG AGCATTTCCG CCGTGCTGCT 
CGGGAGAAGG AGGAGAGGGA AAAGGCCCTG AAGGCTCAGG CTGAGCATGC AGAGAAGGAG 
AAGGAGCGGC TGAGGCAGGA GCGCATGAGG AGCCGAGAGG ATGAAGATGC GCTGGAACAG 
GCACGTCGAG CCCATGAGGA GGCACGACGG CGCCAGGAGC AGCAGCAGCA GCAGCAGCAG 
CAGAGACAGG AGCAGCAGCA GCAGCAGCAG CAGGCAGCTG CTGTGGCCGC TGCCTCTGCC 
CCCCAGGCCC AGAGCTCCCA GCCCCAGTCT ATGCTGGACC AGCAGAGGGA GTTGGCCCGG 
AAACGAGAGC AGGAGCGGAG GCGCAGGGAG GCAATGGCAG CTACAATTGA CATGAATTTC 
CAGAGTGATC TTTTGTCAAT ATTTGAAGAA AATCTTTTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017173077.1
CDS	795..4556
Translation	MAEALEKLFLQKINELPTEETEIMIVQAKGRGRGRKETGRAAKPGVSTVPNTTQASTSPQTQTPQQNPPPPVQATTHPFPAVTPDLIAQPPVMTMVPPQPLQTPSPVPPQPPPPPAPVPQPVQSHPPIIATTPQPVKTKKGVKRKADTTTPTTIDPIHEPPSLAPEPKTAKLGPRRESSRPVKPPKKDVPDSQQHPGPEKSSKISEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYCDIIKHPMDMSTIKSKLESREYRDAQEFGADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPEEPVVTVSSPAVPPPTKVVAPPSSSDSSSDSSSDSDSSTDDSEEERAQRLAELQEQLKAVHEQLAALSQPQQNKPKKKEKDKKEKKKEKHKKKEEVEENKKSKTKELPPKKTKKNNSSNSNVSKKEPVPTKTKPPPTYESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVDVIAGSSKMKGFSSSESESTSESSSSDSEDSETEMAPKSKKKGHTGRDQKKHHHHHHPQMQPAPAPVPQQPPPPPQQPPPPPPPQQQQQQPPPPPPPPSMPQQTAPAMKSSPPPFITAQVPVLEPQLPGSVFDPIGHFTQPILHLPQPELPPHLPQPPEHSTPPHLNQHAVVSPPALHNALPQQPSRPSNRAAALPPKPTRPPAVSPALAQPPLLPQPPMAQPPQVLLEDEEPPAPPLTSMQMQLYLQQLQKVQPPTPLLPSVKVQSQPPPPLPPPPHPSVQQQQLQPQPPPPPPPQPQPPPQQQHQPPPRPVHLPSMPFSAHIQQPPPPPGQQPTHPPPGQQPPPPQPAKPQQVIQHHPSPRHHKSDPYSAGHLREAPSPLMIHSPQMPQFQSLTHQSPPQQNVQPKKQVKGRAEPQPPGPVMGQGQGCPPASPAAVPMLSQELRPPSVVQPQPLVVVKEEKIHSPIIRSEPFSTSLRPEPPKHPENIKAPVHLPQRPEMKPVDIGRPVIRPPEQSAPPPGAPDKDKQKQEPKTPVAPKKDLKIKNMGSWASLVQKHPTTPSSTAKSSSDSFEHFRRAAREKEEREKALKAQAEHAEKEKERLRQERMRSREDEDALEQARRAHEEARRRQEQQQQQQQQRQEQQQQQQQAAAVAAASAPQAQSSQPQSMLDQQRELARKREQERRRREAMAATIDMNFQSDLLSIFEENLF

Target ORF information:

RefSeq Version	XM_017317588.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus bromodomain containing 4 (Brd4), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317588.1

ORF Insert Sequence:

ATGGCAGAAG CTCTGGAGAA GCTCTTCTTG CAAAAAATCA ATGAACTGCC TACAGAAGAA 
ACTGAGATCA TGATAGTCCA GGCAAAAGGA AGAGGACGAG GGAGGAAAGA AACAGGTAGG 
GCAGCAAAGC CTGGTGTATC CACGGTACCA AACACAACTC AAGCATCAAC TTCTCCGCAG 
ACCCAGACGC CTCAGCAGAA CCCTCCTCCA CCTGTGCAGG CCACAACTCA CCCCTTTCCT 
GCTGTCACCC CAGACCTCAT TGCCCAGCCT CCTGTCATGA CAATGGTGCC CCCTCAGCCA 
CTTCAGACTC CTTCACCGGT ACCCCCCCAG CCACCACCCC CACCTGCTCC AGTTCCACAG 
CCTGTGCAGA GTCACCCGCC CATCATTGCG ACCACCCCCC AGCCTGTGAA GACAAAGAAA 
GGGGTGAAGA GGAAAGCAGA TACCACCACC CCTACCACCA TCGACCCCAT TCATGAGCCA 
CCCTCACTGG CCCCAGAGCC CAAGACCGCC AAGCTGGGTC CTCGGCGGGA GAGCAGCAGA 
CCTGTGAAGC CTCCAAAGAA GGATGTACCG GACTCACAGC AGCACCCAGG GCCAGAGAAG 
AGCAGCAAGA TCTCTGAGCA GCTAAAGTGC TGCAGTGGCA TCCTCAAGGA GATGTTTGCC 
AAGAAACATG CTGCCTATGC CTGGCCTTTC TACAAGCCTG TGGATGTGGA GGCACTGGGT 
CTGCACGACT ACTGTGACAT CATCAAACAT CCCATGGACA TGAGCACAAT CAAGTCTAAA 
CTAGAGTCCC GAGAGTACAG AGATGCCCAG GAATTTGGTG CTGATGTCCG ATTGATGTTC 
TCCAACTGCT ACAAGTACAA CCCCCCTGAC CATGAAGTGG TAGCCATGGC TCGAAAACTC 
CAGGATGTGT TTGAAATGCG CTTTGCCAAG ATGCCTGATG AGCCTGAAGA GCCAGTTGTT 
ACAGTGTCCT CTCCTGCAGT GCCACCCCCT ACAAAGGTGG TAGCCCCACC CTCATCTAGT 
GACAGCAGCA GCGACAGTTC TTCCGACAGC GACAGTTCCA CTGACGACTC TGAGGAAGAG 
CGAGCCCAGC GGCTGGCTGA ACTCCAGGAA CAGCTCAAGG CCGTGCATGA GCAGCTTGCA 
GCCCTCTCAC AGCCCCAGCA GAACAAACCA AAGAAAAAGG AGAAGGACAA GAAGGAAAAG 
AAAAAGGAAA AGCACAAAAA GAAAGAAGAA GTGGAGGAAA ATAAAAAAAG CAAAACCAAG 
GAACTTCCTC CCAAAAAGAC AAAGAAAAAT AACAGCAGCA ACAGCAATGT GAGCAAGAAG 
GAACCAGTAC CCACGAAGAC CAAGCCGCCT CCCACATATG AATCAGAAGA GGAGGATAAG 
TGTAAGCCCA TGTCTTATGA GGAGAAGCGG CAGCTAAGTC TAGATATCAA CAAACTTCCT 
GGTGAGAAGC TAGGCCGTGT AGTACACATA ATTCAGTCAA GGGAACCATC ACTTAAAAAC 
TCCAACCCCG ATGAGATTGA GATTGACTTT GAGACCCTGA AGCCATCTAC ACTACGAGAG 
TTGGAGCGAT ATGTCACCTC CTGTTTGCGG AAGAAAAGGA AACCTCAAGC TGAAAAAGTT 
GACGTGATTG CTGGTTCTTC CAAGATGAAG GGATTCTCAT CCTCTGAGTC GGAGAGCACC 
AGCGAATCCA GCTCCTCTGA CAGTGAAGAC TCTGAAACAG AGATGGCTCC CAAGTCAAAA 
AAGAAGGGGC ACACTGGGAG GGACCAGAAA AAGCATCATC ACCATCACCA TCCACAGATG 
CAGCCAGCCC CAGCTCCTGT GCCCCAGCAG CCGCCCCCAC CTCCACAGCA GCCTCCTCCA 
CCCCCACCTC CGCAGCAGCA GCAGCAGCAA CCTCCACCCC CACCACCTCC GCCCTCCATG 
CCACAGCAGA CTGCCCCAGC GATGAAGTCC TCGCCCCCAC CCTTCATCAC TGCCCAGGTC 
CCCGTCCTGG AACCACAGCT GCCAGGCAGT GTCTTTGACC CTATTGGCCA CTTCACCCAG 
CCCATCTTGC ACCTGCCGCA GCCGGAGCTG CCTCCTCACC TGCCCCAGCC ACCTGAGCAC 
AGCACTCCAC CCCATCTCAA CCAGCATGCT GTGGTCTCTC CTCCAGCTTT GCACAATGCG 
CTGCCCCAAC AGCCATCTCG GCCCAGTAAC CGAGCTGCTG CTCTGCCCCC AAAGCCTACC 
CGACCCCCAG CTGTGTCCCC TGCCCTGGCC CAGCCCCCCC TGCTCCCACA ACCACCAATG 
GCTCAGCCCC CCCAAGTGCT GCTGGAGGAT GAAGAGCCAC CTGCCCCACC CCTCACCTCC 
ATGCAGATGC AGTTGTACCT ACAGCAGCTG CAGAAGGTGC AGCCTCCCAC ACCACTACTC 
CCTTCCGTGA AGGTGCAGTC CCAACCCCCA CCGCCTTTGC CGCCTCCACC CCACCCTTCT 
GTGCAGCAGC AGCAGCTCCA GCCACAGCCA CCGCCACCCC CACCTCCTCA GCCACAGCCA 
CCACCCCAGC AACAACACCA GCCTCCACCA CGACCAGTTC ACTTGCCATC CATGCCCTTT 
TCTGCTCATA TTCAGCAGCC CCCACCACCC CCAGGACAGC AACCTACTCA CCCACCCCCA 
GGACAGCAGC CCCCACCACC ACAGCCTGCC AAGCCCCAGC AAGTCATCCA GCATCACCCT 
TCCCCCCGGC ACCACAAGTC AGACCCCTAC TCAGCTGGTC ATCTTCGTGA GGCTCCCTCT 
CCGCTTATGA TACATTCCCC TCAGATGCCA CAGTTCCAGA GCCTGACCCA TCAGTCTCCT 
CCCCAGCAAA ACGTCCAGCC TAAGAAGCAG GTAAAGGGCA GGGCTGAGCC ACAGCCACCA 
GGGCCAGTCA TGGGCCAAGG CCAGGGATGC CCACCTGCCT CACCGGCTGC CGTGCCTATG 
CTGTCCCAGG AGCTGCGACC ACCTTCAGTC GTCCAGCCCC AGCCCCTGGT GGTAGTAAAG 
GAGGAGAAGA TTCACTCACC AATCATTCGC AGCGAGCCTT TCAGCACCTC ACTTCGACCA 
GAGCCCCCCA AGCACCCAGA GAACATCAAG GCCCCAGTCC ACCTGCCCCA GCGGCCTGAG 
ATGAAGCCTG TAGACATAGG GAGGCCTGTG ATCCGGCCTC CAGAGCAGAG TGCACCCCCA 
CCAGGGGCCC CTGACAAGGA CAAACAGAAG CAGGAGCCAA AAACACCAGT GGCACCCAAA 
AAGGACCTGA AAATTAAGAA CATGGGCTCC TGGGCCAGCC TGGTACAGAA GCATCCGACC 
ACCCCATCCT CCACAGCCAA GTCCTCAAGT GACAGCTTTG AGCATTTCCG CCGTGCTGCT 
CGGGAGAAGG AGGAGAGGGA AAAGGCCCTG AAGGCTCAGG CTGAGCATGC AGAGAAGGAG 
AAGGAGCGGC TGAGGCAGGA GCGCATGAGG AGCCGAGAGG ATGAAGATGC GCTGGAACAG 
GCACGTCGAG CCCATGAGGA GGCACGACGG CGCCAGGAGC AGCAGCAGCA GCAGCAGCAG 
CAGAGACAGG AGCAGCAGCA GCAGCAGCAG CAGGCAGCTG CTGTGGCCGC TGCCTCTGCC 
CCCCAGGCCC AGAGCTCCCA GCCCCAGTCT ATGCTGGACC AGCAGAGGGA GTTGGCCCGG 
AAACGAGAGC AGGAGCGGAG GCGCAGGGAG GCAATGGCAG CTACAATTGA CATGAATTTC 
CAGAGTGATC TTTTGTCAAT ATTTGAAGAA AATCTTTTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brd4 ( XM_006524693.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524756.1 Mus musculus bromodomain containing 4 (Brd4), transcript variant X12, mRNA.

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CloneID	OMu53866
Clone ID Related Accession (Same CDS sequence)	XM_006524693.2 , XM_006524693.3 , XM_030249932.1 , XM_030249930.1 , XM_030249931.1
Accession Version	XM_006524693.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3759bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	bromodomain-containing protein 4 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006524693.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAAG CTCTGGAGAA GCTCTTCTTG CAAAAAATCA ATGAACTGCC TACAGAAGAA 
ACTGAGATCA TGATAGTCCA GGCAAAAGGA AGAGGACGAG GGAGGAAAGA AACAGGGGCA 
GCAAAGCCTG GTGTATCCAC GGTACCAAAC ACAACTCAAG CATCAACTTC TCCGCAGACC 
CAGACGCCTC AGCAGAACCC TCCTCCACCT GTGCAGGCCA CAACTCACCC CTTTCCTGCT 
GTCACCCCAG ACCTCATTGC CCAGCCTCCT GTCATGACAA TGGTGCCCCC TCAGCCACTT 
CAGACTCCTT CACCGGTACC CCCCCAGCCA CCACCCCCAC CTGCTCCAGT TCCACAGCCT 
GTGCAGAGTC ACCCGCCCAT CATTGCGACC ACCCCCCAGC CTGTGAAGAC AAAGAAAGGG 
GTGAAGAGGA AAGCAGATAC CACCACCCCT ACCACCATCG ACCCCATTCA TGAGCCACCC 
TCACTGGCCC CAGAGCCCAA GACCGCCAAG CTGGGTCCTC GGCGGGAGAG CAGCAGACCT 
GTGAAGCCTC CAAAGAAGGA TGTACCGGAC TCACAGCAGC ACCCAGGGCC AGAGAAGAGC 
AGCAAGATCT CTGAGCAGCT AAAGTGCTGC AGTGGCATCC TCAAGGAGAT GTTTGCCAAG 
AAACATGCTG CCTATGCCTG GCCTTTCTAC AAGCCTGTGG ATGTGGAGGC ACTGGGTCTG 
CACGACTACT GTGACATCAT CAAACATCCC ATGGACATGA GCACAATCAA GTCTAAACTA 
GAGTCCCGAG AGTACAGAGA TGCCCAGGAA TTTGGTGCTG ATGTCCGATT GATGTTCTCC 
AACTGCTACA AGTACAACCC CCCTGACCAT GAAGTGGTAG CCATGGCTCG AAAACTCCAG 
GATGTGTTTG AAATGCGCTT TGCCAAGATG CCTGATGAGC CTGAAGAGCC AGTTGTTACA 
GTGTCCTCTC CTGCAGTGCC ACCCCCTACA AAGGTGGTAG CCCCACCCTC ATCTAGTGAC 
AGCAGCAGCG ACAGTTCTTC CGACAGCGAC AGTTCCACTG ACGACTCTGA GGAAGAGCGA 
GCCCAGCGGC TGGCTGAACT CCAGGAACAG CTCAAGGCCG TGCATGAGCA GCTTGCAGCC 
CTCTCACAGC CCCAGCAGAA CAAACCAAAG AAAAAGGAGA AGGACAAGAA GGAAAAGAAA 
AAGGAAAAGC ACAAAAAGAA AGAAGAAGTG GAGGAAAATA AAAAAAGCAA AACCAAGGAA 
CTTCCTCCCA AAAAGACAAA GAAAAATAAC AGCAGCAACA GCAATGTGAG CAAGAAGGAA 
CCAGTACCCA CGAAGACCAA GCCGCCTCCC ACATATGAAT CAGAAGAGGA GGATAAGTGT 
AAGCCCATGT CTTATGAGGA GAAGCGGCAG CTAAGTCTAG ATATCAACAA ACTTCCTGGT 
GAGAAGCTAG GCCGTGTAGT ACACATAATT CAGTCAAGGG AACCATCACT TAAAAACTCC 
AACCCCGATG AGATTGAGAT TGACTTTGAG ACCCTGAAGC CATCTACACT ACGAGAGTTG 
GAGCGATATG TCACCTCCTG TTTGCGGAAG AAAAGGAAAC CTCAAGCTGA AAAAGTTGAC 
GTGATTGCTG GTTCTTCCAA GATGAAGGGA TTCTCATCCT CTGAGTCGGA GAGCACCAGC 
GAATCCAGCT CCTCTGACAG TGAAGACTCT GAAACAGAGA TGGCTCCCAA GTCAAAAAAG 
AAGGGGCACA CTGGGAGGGA CCAGAAAAAG CATCATCACC ATCACCATCC ACAGATGCAG 
CCAGCCCCAG CTCCTGTGCC CCAGCAGCCG CCCCCACCTC CACAGCAGCC TCCTCCACCC 
CCACCTCCGC AGCAGCAGCA GCAGCAACCT CCACCCCCAC CACCTCCGCC CTCCATGCCA 
CAGCAGACTG CCCCAGCGAT GAAGTCCTCG CCCCCACCCT TCATCACTGC CCAGGTCCCC 
GTCCTGGAAC CACAGCTGCC AGGCAGTGTC TTTGACCCTA TTGGCCACTT CACCCAGCCC 
ATCTTGCACC TGCCGCAGCC GGAGCTGCCT CCTCACCTGC CCCAGCCACC TGAGCACAGC 
ACTCCACCCC ATCTCAACCA GCATGCTGTG GTCTCTCCTC CAGCTTTGCA CAATGCGCTG 
CCCCAACAGC CATCTCGGCC CAGTAACCGA GCTGCTGCTC TGCCCCCAAA GCCTACCCGA 
CCCCCAGCTG TGTCCCCTGC CCTGGCCCAG CCCCCCCTGC TCCCACAACC ACCAATGGCT 
CAGCCCCCCC AAGTGCTGCT GGAGGATGAA GAGCCACCTG CCCCACCCCT CACCTCCATG 
CAGATGCAGT TGTACCTACA GCAGCTGCAG AAGGTGCAGC CTCCCACACC ACTACTCCCT 
TCCGTGAAGG TGCAGTCCCA ACCCCCACCG CCTTTGCCGC CTCCACCCCA CCCTTCTGTG 
CAGCAGCAGC AGCTCCAGCC ACAGCCACCG CCACCCCCAC CTCCTCAGCC ACAGCCACCA 
CCCCAGCAAC AACACCAGCC TCCACCACGA CCAGTTCACT TGCCATCCAT GCCCTTTTCT 
GCTCATATTC AGCAGCCCCC ACCACCCCCA GGACAGCAAC CTACTCACCC ACCCCCAGGA 
CAGCAGCCCC CACCACCACA GCCTGCCAAG CCCCAGCAAG TCATCCAGCA TCACCCTTCC 
CCCCGGCACC ACAAGTCAGA CCCCTACTCA GCTGGTCATC TTCGTGAGGC TCCCTCTCCG 
CTTATGATAC ATTCCCCTCA GATGCCACAG TTCCAGAGCC TGACCCATCA GTCTCCTCCC 
CAGCAAAACG TCCAGCCTAA GAAGCAGGTA AAGGGCAGGG CTGAGCCACA GCCACCAGGG 
CCAGTCATGG GCCAAGGCCA GGGATGCCCA CCTGCCTCAC CGGCTGCCGT GCCTATGCTG 
TCCCAGGAGC TGCGACCACC TTCAGTCGTC CAGCCCCAGC CCCTGGTGGT AGTAAAGGAG 
GAGAAGATTC ACTCACCAAT CATTCGCAGC GAGCCTTTCA GCACCTCACT TCGACCAGAG 
CCCCCCAAGC ACCCAGAGAA CATCAAGGCC CCAGTCCACC TGCCCCAGCG GCCTGAGATG 
AAGCCTGTAG ACATAGGGAG GCCTGTGATC CGGCCTCCAG AGCAGAGTGC ACCCCCACCA 
GGGGCCCCTG ACAAGGACAA ACAGAAGCAG GAGCCAAAAA CACCAGTGGC ACCCAAAAAG 
GACCTGAAAA TTAAGAACAT GGGCTCCTGG GCCAGCCTGG TACAGAAGCA TCCGACCACC 
CCATCCTCCA CAGCCAAGTC CTCAAGTGAC AGCTTTGAGC ATTTCCGCCG TGCTGCTCGG 
GAGAAGGAGG AGAGGGAAAA GGCCCTGAAG GCTCAGGCTG AGCATGCAGA GAAGGAGAAG 
GAGCGGCTGA GGCAGGAGCG CATGAGGAGC CGAGAGGATG AAGATGCGCT GGAACAGGCA 
CGTCGAGCCC ATGAGGAGGC ACGACGGCGC CAGGAGCAGC AGCAGCAGCA GCAGCAGCAG 
AGACAGGAGC AGCAGCAGCA GCAGCAGCAG GCAGCTGCTG TGGCCGCTGC CTCTGCCCCC 
CAGGCCCAGA GCTCCCAGCC CCAGTCTATG CTGGACCAGC AGAGGGAGTT GGCCCGGAAA 
CGAGAGCAGG AGCGGAGGCG CAGGGAGGCA ATGGCAGCTA CAATTGACAT GAATTTCCAG 
AGTGATCTTT TGTCAATATT TGAAGAAAAT CTTTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524756.1
CDS	836..4594
Translation	MAEALEKLFLQKINELPTEETEIMIVQAKGRGRGRKETGAAKPGVSTVPNTTQASTSPQTQTPQQNPPPPVQATTHPFPAVTPDLIAQPPVMTMVPPQPLQTPSPVPPQPPPPPAPVPQPVQSHPPIIATTPQPVKTKKGVKRKADTTTPTTIDPIHEPPSLAPEPKTAKLGPRRESSRPVKPPKKDVPDSQQHPGPEKSSKISEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYCDIIKHPMDMSTIKSKLESREYRDAQEFGADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPEEPVVTVSSPAVPPPTKVVAPPSSSDSSSDSSSDSDSSTDDSEEERAQRLAELQEQLKAVHEQLAALSQPQQNKPKKKEKDKKEKKKEKHKKKEEVEENKKSKTKELPPKKTKKNNSSNSNVSKKEPVPTKTKPPPTYESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVDVIAGSSKMKGFSSSESESTSESSSSDSEDSETEMAPKSKKKGHTGRDQKKHHHHHHPQMQPAPAPVPQQPPPPPQQPPPPPPPQQQQQQPPPPPPPPSMPQQTAPAMKSSPPPFITAQVPVLEPQLPGSVFDPIGHFTQPILHLPQPELPPHLPQPPEHSTPPHLNQHAVVSPPALHNALPQQPSRPSNRAAALPPKPTRPPAVSPALAQPPLLPQPPMAQPPQVLLEDEEPPAPPLTSMQMQLYLQQLQKVQPPTPLLPSVKVQSQPPPPLPPPPHPSVQQQQLQPQPPPPPPPQPQPPPQQQHQPPPRPVHLPSMPFSAHIQQPPPPPGQQPTHPPPGQQPPPPQPAKPQQVIQHHPSPRHHKSDPYSAGHLREAPSPLMIHSPQMPQFQSLTHQSPPQQNVQPKKQVKGRAEPQPPGPVMGQGQGCPPASPAAVPMLSQELRPPSVVQPQPLVVVKEEKIHSPIIRSEPFSTSLRPEPPKHPENIKAPVHLPQRPEMKPVDIGRPVIRPPEQSAPPPGAPDKDKQKQEPKTPVAPKKDLKIKNMGSWASLVQKHPTTPSSTAKSSSDSFEHFRRAAREKEEREKALKAQAEHAEKEKERLRQERMRSREDEDALEQARRAHEEARRRQEQQQQQQQQRQEQQQQQQQAAAVAAASAPQAQSSQPQSMLDQQRELARKREQERRRREAMAATIDMNFQSDLLSIFEENLF

Target ORF information:

RefSeq Version	XM_006524693.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus bromodomain containing 4 (Brd4), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524693.2

ORF Insert Sequence:

ATGGCAGAAG CTCTGGAGAA GCTCTTCTTG CAAAAAATCA ATGAACTGCC TACAGAAGAA 
ACTGAGATCA TGATAGTCCA GGCAAAAGGA AGAGGACGAG GGAGGAAAGA AACAGGGGCA 
GCAAAGCCTG GTGTATCCAC GGTACCAAAC ACAACTCAAG CATCAACTTC TCCGCAGACC 
CAGACGCCTC AGCAGAACCC TCCTCCACCT GTGCAGGCCA CAACTCACCC CTTTCCTGCT 
GTCACCCCAG ACCTCATTGC CCAGCCTCCT GTCATGACAA TGGTGCCCCC TCAGCCACTT 
CAGACTCCTT CACCGGTACC CCCCCAGCCA CCACCCCCAC CTGCTCCAGT TCCACAGCCT 
GTGCAGAGTC ACCCGCCCAT CATTGCGACC ACCCCCCAGC CTGTGAAGAC AAAGAAAGGG 
GTGAAGAGGA AAGCAGATAC CACCACCCCT ACCACCATCG ACCCCATTCA TGAGCCACCC 
TCACTGGCCC CAGAGCCCAA GACCGCCAAG CTGGGTCCTC GGCGGGAGAG CAGCAGACCT 
GTGAAGCCTC CAAAGAAGGA TGTACCGGAC TCACAGCAGC ACCCAGGGCC AGAGAAGAGC 
AGCAAGATCT CTGAGCAGCT AAAGTGCTGC AGTGGCATCC TCAAGGAGAT GTTTGCCAAG 
AAACATGCTG CCTATGCCTG GCCTTTCTAC AAGCCTGTGG ATGTGGAGGC ACTGGGTCTG 
CACGACTACT GTGACATCAT CAAACATCCC ATGGACATGA GCACAATCAA GTCTAAACTA 
GAGTCCCGAG AGTACAGAGA TGCCCAGGAA TTTGGTGCTG ATGTCCGATT GATGTTCTCC 
AACTGCTACA AGTACAACCC CCCTGACCAT GAAGTGGTAG CCATGGCTCG AAAACTCCAG 
GATGTGTTTG AAATGCGCTT TGCCAAGATG CCTGATGAGC CTGAAGAGCC AGTTGTTACA 
GTGTCCTCTC CTGCAGTGCC ACCCCCTACA AAGGTGGTAG CCCCACCCTC ATCTAGTGAC 
AGCAGCAGCG ACAGTTCTTC CGACAGCGAC AGTTCCACTG ACGACTCTGA GGAAGAGCGA 
GCCCAGCGGC TGGCTGAACT CCAGGAACAG CTCAAGGCCG TGCATGAGCA GCTTGCAGCC 
CTCTCACAGC CCCAGCAGAA CAAACCAAAG AAAAAGGAGA AGGACAAGAA GGAAAAGAAA 
AAGGAAAAGC ACAAAAAGAA AGAAGAAGTG GAGGAAAATA AAAAAAGCAA AACCAAGGAA 
CTTCCTCCCA AAAAGACAAA GAAAAATAAC AGCAGCAACA GCAATGTGAG CAAGAAGGAA 
CCAGTACCCA CGAAGACCAA GCCGCCTCCC ACATATGAAT CAGAAGAGGA GGATAAGTGT 
AAGCCCATGT CTTATGAGGA GAAGCGGCAG CTAAGTCTAG ATATCAACAA ACTTCCTGGT 
GAGAAGCTAG GCCGTGTAGT ACACATAATT CAGTCAAGGG AACCATCACT TAAAAACTCC 
AACCCCGATG AGATTGAGAT TGACTTTGAG ACCCTGAAGC CATCTACACT ACGAGAGTTG 
GAGCGATATG TCACCTCCTG TTTGCGGAAG AAAAGGAAAC CTCAAGCTGA AAAAGTTGAC 
GTGATTGCTG GTTCTTCCAA GATGAAGGGA TTCTCATCCT CTGAGTCGGA GAGCACCAGC 
GAATCCAGCT CCTCTGACAG TGAAGACTCT GAAACAGAGA TGGCTCCCAA GTCAAAAAAG 
AAGGGGCACA CTGGGAGGGA CCAGAAAAAG CATCATCACC ATCACCATCC ACAGATGCAG 
CCAGCCCCAG CTCCTGTGCC CCAGCAGCCG CCCCCACCTC CACAGCAGCC TCCTCCACCC 
CCACCTCCGC AGCAGCAGCA GCAGCAACCT CCACCCCCAC CACCTCCGCC CTCCATGCCA 
CAGCAGACTG CCCCAGCGAT GAAGTCCTCG CCCCCACCCT TCATCACTGC CCAGGTCCCC 
GTCCTGGAAC CACAGCTGCC AGGCAGTGTC TTTGACCCTA TTGGCCACTT CACCCAGCCC 
ATCTTGCACC TGCCGCAGCC GGAGCTGCCT CCTCACCTGC CCCAGCCACC TGAGCACAGC 
ACTCCACCCC ATCTCAACCA GCATGCTGTG GTCTCTCCTC CAGCTTTGCA CAATGCGCTG 
CCCCAACAGC CATCTCGGCC CAGTAACCGA GCTGCTGCTC TGCCCCCAAA GCCTACCCGA 
CCCCCAGCTG TGTCCCCTGC CCTGGCCCAG CCCCCCCTGC TCCCACAACC ACCAATGGCT 
CAGCCCCCCC AAGTGCTGCT GGAGGATGAA GAGCCACCTG CCCCACCCCT CACCTCCATG 
CAGATGCAGT TGTACCTACA GCAGCTGCAG AAGGTGCAGC CTCCCACACC ACTACTCCCT 
TCCGTGAAGG TGCAGTCCCA ACCCCCACCG CCTTTGCCGC CTCCACCCCA CCCTTCTGTG 
CAGCAGCAGC AGCTCCAGCC ACAGCCACCG CCACCCCCAC CTCCTCAGCC ACAGCCACCA 
CCCCAGCAAC AACACCAGCC TCCACCACGA CCAGTTCACT TGCCATCCAT GCCCTTTTCT 
GCTCATATTC AGCAGCCCCC ACCACCCCCA GGACAGCAAC CTACTCACCC ACCCCCAGGA 
CAGCAGCCCC CACCACCACA GCCTGCCAAG CCCCAGCAAG TCATCCAGCA TCACCCTTCC 
CCCCGGCACC ACAAGTCAGA CCCCTACTCA GCTGGTCATC TTCGTGAGGC TCCCTCTCCG 
CTTATGATAC ATTCCCCTCA GATGCCACAG TTCCAGAGCC TGACCCATCA GTCTCCTCCC 
CAGCAAAACG TCCAGCCTAA GAAGCAGGTA AAGGGCAGGG CTGAGCCACA GCCACCAGGG 
CCAGTCATGG GCCAAGGCCA GGGATGCCCA CCTGCCTCAC CGGCTGCCGT GCCTATGCTG 
TCCCAGGAGC TGCGACCACC TTCAGTCGTC CAGCCCCAGC CCCTGGTGGT AGTAAAGGAG 
GAGAAGATTC ACTCACCAAT CATTCGCAGC GAGCCTTTCA GCACCTCACT TCGACCAGAG 
CCCCCCAAGC ACCCAGAGAA CATCAAGGCC CCAGTCCACC TGCCCCAGCG GCCTGAGATG 
AAGCCTGTAG ACATAGGGAG GCCTGTGATC CGGCCTCCAG AGCAGAGTGC ACCCCCACCA 
GGGGCCCCTG ACAAGGACAA ACAGAAGCAG GAGCCAAAAA CACCAGTGGC ACCCAAAAAG 
GACCTGAAAA TTAAGAACAT GGGCTCCTGG GCCAGCCTGG TACAGAAGCA TCCGACCACC 
CCATCCTCCA CAGCCAAGTC CTCAAGTGAC AGCTTTGAGC ATTTCCGCCG TGCTGCTCGG 
GAGAAGGAGG AGAGGGAAAA GGCCCTGAAG GCTCAGGCTG AGCATGCAGA GAAGGAGAAG 
GAGCGGCTGA GGCAGGAGCG CATGAGGAGC CGAGAGGATG AAGATGCGCT GGAACAGGCA 
CGTCGAGCCC ATGAGGAGGC ACGACGGCGC CAGGAGCAGC AGCAGCAGCA GCAGCAGCAG 
AGACAGGAGC AGCAGCAGCA GCAGCAGCAG GCAGCTGCTG TGGCCGCTGC CTCTGCCCCC 
CAGGCCCAGA GCTCCCAGCC CCAGTCTATG CTGGACCAGC AGAGGGAGTT GGCCCGGAAA 
CGAGAGCAGG AGCGGAGGCG CAGGGAGGCA ATGGCAGCTA CAATTGACAT GAATTTCCAG 
AGTGATCTTT TGTCAATATT TGAAGAAAAT CTTTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Brd4
Entrez Gene ID	57261
Full Name	bromodomain containing 4
Synonyms	Brd5,HUNK1,MCAP,WI-11513
General protein information	Preferred Names bromodomain containing 4 Names bromodomain-containing protein 4 bromodomain-containing 5 fsh-like protein mitotic chromosome-associated protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17 B1\|17 17.39 cM
Summary	This gene was temporarily named bromodomain-containing 5 (Brd5) and was renamed bromodomain-containing 4 (Brd4). [provided by RefSeq, Jul 2008].

Mus musculus (house mouse)	Brd4	NP_065254.3
Xenopus tropicalis (tropical clawed frog)	brd4	NP_001135690.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Brd4 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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