Lamtor3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Lamtor3
Entrez Gene ID	56692
Full Name	late endosomal/lysosomal adaptor, MAPK and MTOR activator 3
Synonyms	AW556229,Map2k1ip1,Mapbp,Mapksp1,Mp1
General protein information	Preferred Names late endosomal/lysosomal adaptor, MAPK and MTOR activator 3 Names ragulator complex protein LAMTOR3 MAPK scaffold protein 1 MEK binding partner 1 late endosomal/lysosomal adaptor and MAPK and MTOR activator 3 mitogen activated protein, binding protein mitogen-activated protein kinase kinase 1-interacting protein 1 mitogen-activated protein kinase scaffold protein 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 3 Map Location: 3\|3 G3

mRNA and Protein(s)

mRNA	Protein	Name
NM_019920.2	NP_064304.1	ragulator complex protein LAMTOR3

Pathways from BioSystems

Homologies

Homo sapiens (human)	LAMTOR3	NP_068805.1
Canis lupus familiaris (dog)	LAMTOR3	XP_862361.1
Rattus norvegicus (Norway rat)	Lamtor3	NP_001008376.1
Xenopus tropicalis (tropical clawed frog)	lamtor3	NP_001135566.1
Pan troglodytes (chimpanzee)	LAMTOR3	XP_517362.2
Bos taurus (cattle)	LAMTOR3	NP_001069450.1
Mus musculus (house mouse)	Lamtor3	NP_064304.1
Danio rerio (zebrafish)	lamtor3	NP_001018410.1
	MAPKSP1	XP_001107957.1
Gallus gallus (chicken)	LAMTOR3	NP_001006559.1
Drosophila melanogaster (fruit fly)	CG5110	NP_001246062.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000997	XP_309192.2

Gene Ontology
Bibliography

Related articles in PubMed

The late endosomal p14-MP1 (LAMTOR2/3) complex regulates focal adhesion dynamics during cell migration.
Schiefermeier N, Scheffler JM, de Araujo ME, Stasyk T, Yordanov T, Ebner HL, Offterdinger M, Munck S, Hess MW, Wickstr?m SA, Lange A, Wunderlich W, F?ssler R, Teis D, Huber LA
The Journal of cell biology205(4)525-40(2014 May)

Targeted extracellular signal-regulated kinase activation mediated by Leishmania amazonensis requires MP1 scaffold.
Boggiatto PM, Martinez PA, Pullikuth A, Jones DE, Bellaire B, Catling A, Petersen C
Microbes and infection16(4)328-36(2014 Apr)

Stability of the endosomal scaffold protein LAMTOR3 depends on heterodimer assembly and proteasomal degradation.
de Ara?jo ME, Stasyk T, Taub N, Ebner HL, F?rst B, Filipek P, Weys SR, Hess MW, Lindner H, Kremser L, Huber LA
The Journal of biological chemistry288(25)18228-42(2013 Jun)

A genome-wide RNAi screen in mouse embryonic stem cells identifies Mp1 as a key mediator of differentiation.
Westerman BA, Braat AK, Taub N, Potman M, Vissers JH, Blom M, Verhoeven E, Stoop H, Gillis A, Velds A, Nijkamp W, Beijersbergen R, Huber LA, Looijenga LH, van Lohuizen M
The Journal of experimental medicine208(13)2675-89(2011 Dec)

The novel lipid raft adaptor p18 controls endosome dynamics by anchoring the MEK-ERK pathway to late endosomes.
Nada S, Hondo A, Kasai A, Koike M, Saito K, Uchiyama Y, Okada M
The EMBO journal28(5)477-89(2009 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We show an essential function for late endosomes carrying the p14-MP1 (LAMTOR2/3) complex in focal adhesion dynamics.
Title: The late endosomal p14-MP1 (LAMTOR2/3) complex regulates focal adhesion dynamics during cell migration.

This novel mechanism for localization of intracellular Leishmania amazonensis-mediated ERK1/2 phosphorylation required the endosomal scaffold protein MP1 and localized to Leishmania amazonensis parasitophorous vacuoles.
Title: Targeted extracellular signal-regulated kinase activation mediated by Leishmania amazonensis requires MP1 scaffold.

Data indicate that LAMTOR3 protein is stabilized by associating with LAMTOR2.
Title: Stability of the endosomal scaffold protein LAMTOR3 depends on heterodimer assembly and proteasomal degradation.

A genome-wide RNAi screen in mouse embryonic stem cells identifies Mp1 as a key mediator of differentiation.
Title: A genome-wide RNAi screen in mouse embryonic stem cells identifies Mp1 as a key mediator of differentiation.

MP1 and the MP1*P14 complex have the potential to differentially modulate activating and inhibiting signals in the Raf-MKK1/2-ERK1/2 pathway.
Title: Regulation of protein phosphorylation within the MKK1-ERK2 complex by MP1 and the MP1*P14 heterodimer.

The following Lamtor3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Lamtor3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu11804	NM_019920.2 Latest version!	Mus musculus late endosomal/lysosomal adaptor, MAPK and MTOR activator 3 (Lamtor3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Lamtor3 ( NM_019920.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_064304.1 Mus musculus late endosomal/lysosomal adaptor, MAPK and MTOR activator 3 (Lamtor3), mRNA.

Price & Availability↑

CloneID	OMu11804
Clone ID Related Accession (Same CDS sequence)	NM_019920.2
Accession Version	NM_019920.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 375bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	ragulator complex protein LAMTOR3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC146607.2. On Nov 17, 2006 this sequence version replaced NM_019920.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF082526.1, AK151432.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGATG ATCTAAAGCG ATTCCTGTAC AAAAAGTTGC CAAGTGTTGA AGGGCTCCAT 
GCAATTGTTG TGTCAGATAG AGACGGGGTG CCGGTTATTA AAGTGGCTAA CGACAGTGCC 
CCAGAGCACG CCCTGAGACC TGGCTTCCTA TCCACGTTTG CCCTCGCAAC AGACCAAGGC 
AGCAAACTCG GACTTTCAAA AAACAAAAGC ATCATCTGCT ACTATAACAC CTACCAGGTG 
GTTCAGTTCA ATCGTTTGCC TCTGGTGGTG AGTTTCATAG CCAGCAGCAG TGCCAACACA 
GGACTAATTG TCAGCCTAGA AAAGGAGCTA GCTCCGTTAT TTGAAGAACT GATAAAAGTT 
GTGGAAGTGT CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_064304.1
CDS	147..521
Translation	MADDLKRFLYKKLPSVEGLHAIVVSDRDGVPVIKVANDSAPEHALRPGFLSTFALATDQGSKLGLSKNKSIICYYNTYQVVQFNRLPLVVSFIASSSANTGLIVSLEKELAPLFEELIKVVEVS

Target ORF information:

RefSeq Version	NM_019920.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus late endosomal/lysosomal adaptor, MAPK and MTOR activator 3 (Lamtor3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019920.2

ORF Insert Sequence:

ATGGCGGATG ATCTAAAGCG ATTCCTGTAC AAAAAGTTGC CAAGTGTTGA AGGGCTCCAT 
GCAATTGTTG TGTCAGATAG AGACGGGGTG CCGGTTATTA AAGTGGCTAA CGACAGTGCC 
CCAGAGCACG CCCTGAGACC TGGCTTCCTA TCCACGTTTG CCCTCGCAAC AGACCAAGGC 
AGCAAACTCG GACTTTCAAA AAACAAAAGC ATCATCTGCT ACTATAACAC CTACCAGGTG 
GTTCAGTTCA ATCGTTTGCC TCTGGTGGTG AGTTTCATAG CCAGCAGCAG TGCCAACACA 
GGACTAATTG TCAGCCTAGA AAAGGAGCTA GCTCCGTTAT TTGAAGAACT GATAAAAGTT 
GTGGAAGTGT CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The late endosomal p14-MP1 (LAMTOR2/3) complex regulates focal adhesion dynamics during cell migration.
The Journal of cell biology205(4)525-40(2014 May)
Schiefermeier N,Scheffler JM,de Araujo ME,Stasyk T,Yordanov T,Ebner HL,Offterdinger M,Munck S,Hess MW,Wickstr?m SA,Lange A,Wunderlich W,F?ssler R,Teis D,Huber LA

Targeted extracellular signal-regulated kinase activation mediated by Leishmania amazonensis requires MP1 scaffold.
Microbes and infection16(4)328-36(2014 Apr)
Boggiatto PM,Martinez PA,Pullikuth A,Jones DE,Bellaire B,Catling A,Petersen C

Stability of the endosomal scaffold protein LAMTOR3 depends on heterodimer assembly and proteasomal degradation.
The Journal of biological chemistry288(25)18228-42(2013 Jun)
de Ara?jo ME,Stasyk T,Taub N,Ebner HL,F?rst B,Filipek P,Weys SR,Hess MW,Lindner H,Kremser L,Huber LA

A genome-wide RNAi screen in mouse embryonic stem cells identifies Mp1 as a key mediator of differentiation.
The Journal of experimental medicine208(13)2675-89(2011 Dec)
Westerman BA,Braat AK,Taub N,Potman M,Vissers JH,Blom M,Verhoeven E,Stoop H,Gillis A,Velds A,Nijkamp W,Beijersbergen R,Huber LA,Looijenga LH,van Lohuizen M

The novel lipid raft adaptor p18 controls endosome dynamics by anchoring the MEK-ERK pathway to late endosomes.
The EMBO journal28(5)477-89(2009 Mar)
Nada S,Hondo A,Kasai A,Koike M,Saito K,Uchiyama Y,Okada M