Dnajb8 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dnajb8
Entrez Gene ID	56691
Full Name	DnaJ heat shock protein family (Hsp40) member B8
Synonyms	1700016F14Rik,1700031F10Rik,AI326313,mDj6
General protein information	Preferred Names DnaJ heat shock protein family (Hsp40) member B8 Names dnaJ homolog subfamily B member 8 DnaJ (Hsp40) homolog, subfamily B, member 8 DnaJ homolog 6
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6 D1\|6 39.25 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_019964.1	NP_064348.1	dnaJ homolog subfamily B member 8

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	DNAJB8	XP_526299.1
Bos taurus (cattle)	DNAJB8	XP_002697194.1
Mus musculus (house mouse)	Dnajb8	NP_064348.1
Homo sapiens (human)	DNAJB8	NP_699161.1
	DNAJB8	XP_001097989.2
Canis lupus familiaris (dog)	DNAJB8	XP_851811.1
Rattus norvegicus (Norway rat)	Dnajb8	NP_001102718.1

Gene Ontology
Bibliography

Related articles in PubMed

Dnajb8, a Member of the Heat Shock Protein 40 Family Has a Role in the Tumor Initiation and Resistance to Docetaxel but Is Dispensable for Stress Response.
Yamashita M, Hirohashi Y, Torigoe T, Kusumoto H, Murai A, Imagawa T, Sato N
PloS one11(1)e0146501(2016)

HSP DNAJB8 controls tumor-initiating ability in renal cancer stem-like cells.
Nishizawa S, Hirohashi Y, Torigoe T, Takahashi A, Tamura Y, Mori T, Kanaseki T, Kamiguchi K, Asanuma H, Morita R, Sokolovskaya A, Matsuzaki J, Yamada R, Fujii R, Kampinga HH, Kondo T, Hasegawa T, Hara I, Sato N
Cancer research72(11)2844-54(2012 Jun)

Identifying new candidate genes for hereditary facial paresis on chromosome 3q21-q22 by RNA in situ hybridization in mouse.
van der Zwaag B, Burbach JP, Scharfe C, Oefner PJ, Brunner HG, Padberg GW, van Bokhoven H
Genomics86(1)55-67(2005 Jul)

Multiple, distant Gata2 enhancers specify temporally and tissue-specific patterning in the developing urogenital system.
Khandekar M, Suzuki N, Lewton J, Yamamoto M, Engel JD
Molecular and cellular biology24(23)10263-76(2004 Dec)

Mammalian HSP40/DNAJ homologs: cloning of novel cDNAs and a proposal for their classification and nomenclature.
Ohtsuka K, Hata M
Cell stress & chaperones5(2)98-112(2000 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Dnajb8 has a role in tumor initiation, side population ratio and sphere formation but it is dispensable for stress responses
Title: Dnajb8, a Member of the Heat Shock Protein 40 Family Has a Role in the Tumor Initiation and Resistance to Docetaxel but Is Dispensable for Stress Response.

findings suggest that DNAJB8 plays a role in Cancer stem-like cell (CSC) maintenance and that it offers a candidate for CSC-targeting immunotherapy in renal cell carcinoma
Title: HSP DNAJB8 controls tumor-initiating ability in renal cancer stem-like cells.

The following Dnajb8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dnajb8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu58092	NM_019964.1 Latest version!	Mus musculus DnaJ heat shock protein family (Hsp40) member B8 (Dnajb8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dnajb8 ( NM_019964.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_064348.1 Mus musculus DnaJ heat shock protein family (Hsp40) member B8 (Dnajb8), mRNA.

Price & Availability↑

CloneID	OMu58092
Clone ID Related Accession (Same CDS sequence)	NM_019964.1
Accession Version	NM_019964.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 684bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	dnaJ homolog subfamily B member 8
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB028856.1. On May 4, 2006 this sequence version replaced XM_981913.1. ##Evidence-Data-START## Transcript is intronless :: AB028856.1 [ECO:0000345] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCAACT ACTATGAAGT GCTGGGTGTA CAATCAAGCG CCTCCCCTGA GGACATCAAG 
AAGGCCTATC GAAAGCTGGC TTTGCGTTGG CACCCTGACA AGAACCCCGA CAACAAAGAA 
GAAGCTGAGA AGAAGTTTAA GCAGGTTTCC GAAGCCTACG AAGTCTTGTC AGACTCTAAG 
AAGCGTTCTG TGTACGACCG GGCAGGCTGT GACAGATGGA GAGCAGGTGG CGGTGCCAAT 
GTCCCCCATA GCAGTCCCTT CGGTGCTGGC TATCCCTTCC GAAATCCTGA GGATATTTTC 
CGCGAGTTCT TTGGGGGACT GGACCCTTTT TCCTTTGAAT TCTGGGACAC TCCGTTCAGC 
GGCCGAGGCC GGCCCCATGG TTTACACAGA GTCTTCCCTT CGGGCTTTGG TGAGTTCCCA 
GCCTTCATGG AAGCCTTGTC ATCTTTCAAT ACTCTGGGCC ATGGTGGGGG CAGCCGCTCC 
ACCTTTTCAT CTGCTTCGTT TGGTGGCTCT GGCAGCTCAG GGTTCAAGTC AGTGATGTCA 
TCGACTGAGA TGGTGAACGG CCGTAAGGTG ACCACCAAGC GAATCATTGA GAACGGCCAA 
GAGCGGGTGG AGGTGGAAGA AGATGGACAG CTCCGGTCAG TAACTGTCAA TGGCAAGGAG 
AAGCTCATGA GGGTGGACAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_064348.1
CDS	217..900
Translation	MANYYEVLGVQSSASPEDIKKAYRKLALRWHPDKNPDNKEEAEKKFKQVSEAYEVLSDSKKRSVYDRAGCDRWRAGGGANVPHSSPFGAGYPFRNPEDIFREFFGGLDPFSFEFWDTPFSGRGRPHGLHRVFPSGFGEFPAFMEALSSFNTLGHGGGSRSTFSSASFGGSGSSGFKSVMSSTEMVNGRKVTTKRIIENGQERVEVEEDGQLRSVTVNGKEKLMRVDK

Target ORF information:

RefSeq Version	NM_019964.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus DnaJ heat shock protein family (Hsp40) member B8 (Dnajb8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019964.1

ORF Insert Sequence:

ATGGCCAACT ACTATGAAGT GCTGGGTGTA CAATCAAGCG CCTCCCCTGA GGACATCAAG 
AAGGCCTATC GAAAGCTGGC TTTGCGTTGG CACCCTGACA AGAACCCCGA CAACAAAGAA 
GAAGCTGAGA AGAAGTTTAA GCAGGTTTCC GAAGCCTACG AAGTCTTGTC AGACTCTAAG 
AAGCGTTCTG TGTACGACCG GGCAGGCTGT GACAGATGGA GAGCAGGTGG CGGTGCCAAT 
GTCCCCCATA GCAGTCCCTT CGGTGCTGGC TATCCCTTCC GAAATCCTGA GGATATTTTC 
CGCGAGTTCT TTGGGGGACT GGACCCTTTT TCCTTTGAAT TCTGGGACAC TCCGTTCAGC 
GGCCGAGGCC GGCCCCATGG TTTACACAGA GTCTTCCCTT CGGGCTTTGG TGAGTTCCCA 
GCCTTCATGG AAGCCTTGTC ATCTTTCAAT ACTCTGGGCC ATGGTGGGGG CAGCCGCTCC 
ACCTTTTCAT CTGCTTCGTT TGGTGGCTCT GGCAGCTCAG GGTTCAAGTC AGTGATGTCA 
TCGACTGAGA TGGTGAACGG CCGTAAGGTG ACCACCAAGC GAATCATTGA GAACGGCCAA 
GAGCGGGTGG AGGTGGAAGA AGATGGACAG CTCCGGTCAG TAACTGTCAA TGGCAAGGAG 
AAGCTCATGA GGGTGGACAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Dnajb8, a Member of the Heat Shock Protein 40 Family Has a Role in the Tumor Initiation and Resistance to Docetaxel but Is Dispensable for Stress Response.
PloS one11(1)e0146501(2016)
Yamashita M,Hirohashi Y,Torigoe T,Kusumoto H,Murai A,Imagawa T,Sato N

HSP DNAJB8 controls tumor-initiating ability in renal cancer stem-like cells.
Cancer research72(11)2844-54(2012 Jun)
Nishizawa S,Hirohashi Y,Torigoe T,Takahashi A,Tamura Y,Mori T,Kanaseki T,Kamiguchi K,Asanuma H,Morita R,Sokolovskaya A,Matsuzaki J,Yamada R,Fujii R,Kampinga HH,Kondo T,Hasegawa T,Hara I,Sato N

Identifying new candidate genes for hereditary facial paresis on chromosome 3q21-q22 by RNA in situ hybridization in mouse.
Genomics86(1)55-67(2005 Jul)
van der Zwaag B,Burbach JP,Scharfe C,Oefner PJ,Brunner HG,Padberg GW,van Bokhoven H

Multiple, distant Gata2 enhancers specify temporally and tissue-specific patterning in the developing urogenital system.
Molecular and cellular biology24(23)10263-76(2004 Dec)
Khandekar M,Suzuki N,Lewton J,Yamamoto M,Engel JD

Mammalian HSP40/DNAJ homologs: cloning of novel cDNAs and a proposal for their classification and nomenclature.
Cell stress & chaperones5(2)98-112(2000 Apr)
Ohtsuka K,Hata M