Sphk2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sphk2
Entrez Gene ID	56632
Full Name	sphingosine kinase 2
Synonyms	C76851
General protein information	Preferred Names sphingosine kinase 2 Names sphingosine kinase 2 SK 2 SPK 2 Spk2, Sk2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 B3
Summary	This gene encodes a kinase that phosphorylates sphingosine into sphingosine-1-phosphate, which is involved in cell differentiation, motility, and apoptosis. The encoded protein plays a role in maintaining cellular levels of sphingosine-1-phosphate. The gene product also enhances apoptosis in different cell types and suppresses cellular proliferation. In mast cells, the encoded protein is necessary for influx of calcium, protein kinase C activation, and cytokine production and degranulation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001172561.1	NP_001166032.1	sphingosine kinase 2
XM_006541012.3	XP_006541075.1	sphingosine kinase 2 isoform X1
NM_020011.5	NP_064395.2	sphingosine kinase 2
XM_006541012.2	XP_006541075.1	sphingosine kinase 2 isoform X1
XM_006541013.2	XP_006541076.1	sphingosine kinase 2 isoform X1
XM_006541013.3	XP_006541076.1	sphingosine kinase 2 isoform X1
NM_203280.3	NP_975009.1	sphingosine kinase 2
XM_006541012.4	XP_006541075.1	sphingosine kinase 2 isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	SPHK2	XP_002695244.2
Homo sapiens (human)	SPHK2	NP_064511.2
Pan troglodytes (chimpanzee)	SPHK2	XP_001171404.2
Danio rerio (zebrafish)	sphk2	XP_003200262.2
	SPHK2	XP_001113908.1
Mus musculus (house mouse)	Sphk2	NP_064395.2
Rattus norvegicus (Norway rat)	Sphk2	NP_001012066.1
Drosophila melanogaster (fruit fly)	Sk1	NP_572717.1
Caenorhabditis elegans (roundworm)	sphk-1	NP_001022018.1
Xenopus tropicalis (tropical clawed frog)	sphk2	XP_002938325.1
Canis lupus familiaris (dog)	SPHK2	XP_005616402.1

Gene Ontology

The following Sphk2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sphk2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu16495	NM_020011.5 Latest version!	Mus musculus sphingosine kinase 2 (Sphk2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OMu16495	NM_001172561.1 Latest version!	Mus musculus sphingosine kinase 2 (Sphk2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OMu16495	NM_203280.3 Latest version!	Mus musculus sphingosine kinase 2 (Sphk2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OMu16495	XM_006541012.4 Latest version!	Mus musculus sphingosine kinase 2 (Sphk2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu16495	XM_006541013.3 Latest version!	Mus musculus sphingosine kinase 2 (Sphk2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
View Old Accession Versions >>
OMu16495	XM_006541012.2	Mus musculus sphingosine kinase 2 (Sphk2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OMu16495	XM_006541013.2	Mus musculus sphingosine kinase 2 (Sphk2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OMu16495	XM_006541012.3	Mus musculus sphingosine kinase 2 (Sphk2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sphk2 ( NM_001172561.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001166032.1 Mus musculus sphingosine kinase 2 (Sphk2), transcript variant 3, mRNA.

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CloneID	OMu16495
Clone ID Related Accession (Same CDS sequence)	NM_001172561.1 , XM_006541012.3 , NM_020011.5 , XM_006541012.2 , XM_006541013.2 , XM_006541013.3 , NM_203280.3 , XM_006541012.4
Accession Version	NM_001172561.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1854bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-04
Organism	Mus musculus(house mouse)
Product	sphingosine kinase 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC167242.4. Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK146414.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCCCAC CACCACTACT GCCAGTGGCT GCCAGCACTC CAATCCTGCA CGGCGAGTTT 
GGTTCCTACC CGGCCAACGG CCCACGGTTT GCCCTCACCC TCACAACACA AGCCCTACAC 
ATACAGCGAC TACGCCCAAA GCCAGAAGCC CGGCCCCGAG ATGGTCTAGT CTCTCTGGAT 
GAGGTCTCGG GCTGTGGCAC CCTGCAGAGC CGTAGCCCCG AGGACACTGC AGCCTACTTC 
TGCATCTACA CCTACCCACG TGGCCGTCGA GGGGGCCGGC GCAGAGCTAC GCGGACCTTC 
CGGGCGGATG GGGCCACCAC TTATGAGGAG AATCGTGCAG AGGCCCAGCG CTGGGCCACT 
GCCCTCACGT GTCTCCTCCG AGGAGTGCCT CTGTCAGGGG ACCAGGAAAT CACCCCTGAA 
TTGCTGCCCC GGAAGCCCAG GCTGCTCATA TTGGTCAATC CCTTTGGGGG GCGGGGCCTG 
GCCTGGCAGC GCTGTATGGA CCACGTGGTG CCAATGATCT CTGAAGCTGG GCTGTCCTTC 
AACCTCATAC AGACAGAACG ACAGAACCAT GCCCGTGAGC TGGTGCAGGG GTTAAGCCTG 
AGTGAGTGGG AAGGCATTGT CACTGTGTCT GGAGACGGGC TGCTTTACGA GGTGCTGAAT 
GGGCTCCTTG ATCGGCCAGA CTGGGAGGAT GCCGTGCGGA TGCCCATTGG TGTCCTCCCC 
TGTGGATCGG GCAATGCGCT AGCTGGGGCG GTGAACCATC ATGGCGGGTT TGAGCAGGTT 
GTCGGTGTTG ACCTGTTGCT CAACTGCTCG CTTCTTCTCT GCCGTGGTGG CAGCCATCCT 
CTGGACCTGC TCTCTGTGAC GCTAGCCTCG GGATCCCGCT GTTTTTCCTT CCTGTCAGTG 
GCCTGGGGAT TCTTGTCAGA TGTGGACATT CACAGTGAGC GCTTCAGGGC CCTGGGCAGC 
GCTCGATTCA CACTGGGTGC AGTGCTAGGC CTGGCCTCGT TGCATACCTA CCGTGGACGC 
CTCTCCTACC TCCCCGCTAC CACAGAACCA GCCTTGCCCA TCCCAGGCCA CAGTCTGCCT 
CGAGCCAAGT CAGAACTAGT CTTGGCTCCA GCCCCAGCCC CCGCCGCCAC CCACTCGCCT 
CTACATCGAT CTGTGTCTGA CCTGCCCCTG CCCCTTCCCC AGCCTGCCTT GGTCTCCCCT 
GGCTCCCCTG AGCCCCTGCC TGACCTGTCC CTCAATGGTG GTGGTCCAGA GCTGACTGGA 
GACTGGGGAG GAGCTGGGGA TGCACCTCTG TCCCCAGACC CACTGCTGCC TTCATCCCCC 
AACGCTCTCA AAACAGCTCA GCTTTCACCC ATCGCTGAAG GGCCCCCAGA AATGCCAGCA 
TCTTCGGGGT TCCTGCCTCC CACCCACAGT GCCCCAGAAG CCTCTACCTG GGGCCCAGTG 
GACCACCTCC TCCCTCCCCT GGGCTCTCCA CTGCCCCAAG ACTGGGTGAC AATAGAGGGG 
GAGTTTGTAC TCATGTTGGG CATCTTGCCG AGCCACCTCT GCGCAGACCT GATGGCAGCC 
CCACATGCAC GCTTTGATGA TGGCGTTGTG CACCTGTGTT GGGTGCGGAG CGGCATCTCA 
CGGGCTGCAC TTCTACGCAT TTTGCTGGCC ATGGAGCATG GAAACCACTT CAGCCTGGGC 
TGCCCCCATC TGGGCTATGC TGCAGCACGT GCCTTCCGCC TTGAACCACT CACGCCTCGT 
GGCCTGCTCA CTGTAGATGG GGAGTTAGTG GAGTATGGGC CAATACAGGC GCAGGTGCAC 
CCAGGTCTCG CCACGCTGCT CACTGGGCCT GCAGGTCAAA AGCCACAAGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001166032.1
CDS	366..2219
Translation	MAPPPLLPVAASTPILHGEFGSYPANGPRFALTLTTQALHIQRLRPKPEARPRDGLVSLDEVSGCGTLQSRSPEDTAAYFCIYTYPRGRRGGRRRATRTFRADGATTYEENRAEAQRWATALTCLLRGVPLSGDQEITPELLPRKPRLLILVNPFGGRGLAWQRCMDHVVPMISEAGLSFNLIQTERQNHARELVQGLSLSEWEGIVTVSGDGLLYEVLNGLLDRPDWEDAVRMPIGVLPCGSGNALAGAVNHHGGFEQVVGVDLLLNCSLLLCRGGSHPLDLLSVTLASGSRCFSFLSVAWGFLSDVDIHSERFRALGSARFTLGAVLGLASLHTYRGRLSYLPATTEPALPIPGHSLPRAKSELVLAPAPAPAATHSPLHRSVSDLPLPLPQPALVSPGSPEPLPDLSLNGGGPELTGDWGGAGDAPLSPDPLLPSSPNALKTAQLSPIAEGPPEMPASSGFLPPTHSAPEASTWGPVDHLLPPLGSPLPQDWVTIEGEFVLMLGILPSHLCADLMAAPHARFDDGVVHLCWVRSGISRAALLRILLAMEHGNHFSLGCPHLGYAAARAFRLEPLTPRGLLTVDGELVEYGPIQAQVHPGLATLLTGPAGQKPQA

Target ORF information:

RefSeq Version	NM_001172561.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sphingosine kinase 2 (Sphk2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001172561.1

ORF Insert Sequence:

ATGGCCCCAC CACCACTACT GCCAGTGGCT GCCAGCACTC CAATCCTGCA CGGCGAGTTT 
GGTTCCTACC CGGCCAACGG CCCACGGTTT GCCCTCACCC TCACAACACA AGCCCTACAC 
ATACAGCGAC TACGCCCAAA GCCAGAAGCC CGGCCCCGAG ATGGTCTAGT CTCTCTGGAT 
GAGGTCTCGG GCTGTGGCAC CCTGCAGAGC CGTAGCCCCG AGGACACTGC AGCCTACTTC 
TGCATCTACA CCTACCCACG TGGCCGTCGA GGGGGCCGGC GCAGAGCTAC GCGGACCTTC 
CGGGCGGATG GGGCCACCAC TTATGAGGAG AATCGTGCAG AGGCCCAGCG CTGGGCCACT 
GCCCTCACGT GTCTCCTCCG AGGAGTGCCT CTGTCAGGGG ACCAGGAAAT CACCCCTGAA 
TTGCTGCCCC GGAAGCCCAG GCTGCTCATA TTGGTCAATC CCTTTGGGGG GCGGGGCCTG 
GCCTGGCAGC GCTGTATGGA CCACGTGGTG CCAATGATCT CTGAAGCTGG GCTGTCCTTC 
AACCTCATAC AGACAGAACG ACAGAACCAT GCCCGTGAGC TGGTGCAGGG GTTAAGCCTG 
AGTGAGTGGG AAGGCATTGT CACTGTGTCT GGAGACGGGC TGCTTTACGA GGTGCTGAAT 
GGGCTCCTTG ATCGGCCAGA CTGGGAGGAT GCCGTGCGGA TGCCCATTGG TGTCCTCCCC 
TGTGGATCGG GCAATGCGCT AGCTGGGGCG GTGAACCATC ATGGCGGGTT TGAGCAGGTT 
GTCGGTGTTG ACCTGTTGCT CAACTGCTCG CTTCTTCTCT GCCGTGGTGG CAGCCATCCT 
CTGGACCTGC TCTCTGTGAC GCTAGCCTCG GGATCCCGCT GTTTTTCCTT CCTGTCAGTG 
GCCTGGGGAT TCTTGTCAGA TGTGGACATT CACAGTGAGC GCTTCAGGGC CCTGGGCAGC 
GCTCGATTCA CACTGGGTGC AGTGCTAGGC CTGGCCTCGT TGCATACCTA CCGTGGACGC 
CTCTCCTACC TCCCCGCTAC CACAGAACCA GCCTTGCCCA TCCCAGGCCA CAGTCTGCCT 
CGAGCCAAGT CAGAACTAGT CTTGGCTCCA GCCCCAGCCC CCGCCGCCAC CCACTCGCCT 
CTACATCGAT CTGTGTCTGA CCTGCCCCTG CCCCTTCCCC AGCCTGCCTT GGTCTCCCCT 
GGCTCCCCTG AGCCCCTGCC TGACCTGTCC CTCAATGGTG GTGGTCCAGA GCTGACTGGA 
GACTGGGGAG GAGCTGGGGA TGCACCTCTG TCCCCAGACC CACTGCTGCC TTCATCCCCC 
AACGCTCTCA AAACAGCTCA GCTTTCACCC ATCGCTGAAG GGCCCCCAGA AATGCCAGCA 
TCTTCGGGGT TCCTGCCTCC CACCCACAGT GCCCCAGAAG CCTCTACCTG GGGCCCAGTG 
GACCACCTCC TCCCTCCCCT GGGCTCTCCA CTGCCCCAAG ACTGGGTGAC AATAGAGGGG 
GAGTTTGTAC TCATGTTGGG CATCTTGCCG AGCCACCTCT GCGCAGACCT GATGGCAGCC 
CCACATGCAC GCTTTGATGA TGGCGTTGTG CACCTGTGTT GGGTGCGGAG CGGCATCTCA 
CGGGCTGCAC TTCTACGCAT TTTGCTGGCC ATGGAGCATG GAAACCACTT CAGCCTGGGC 
TGCCCCCATC TGGGCTATGC TGCAGCACGT GCCTTCCGCC TTGAACCACT CACGCCTCGT 
GGCCTGCTCA CTGTAGATGG GGAGTTAGTG GAGTATGGGC CAATACAGGC GCAGGTGCAC 
CCAGGTCTCG CCACGCTGCT CACTGGGCCT GCAGGTCAAA AGCCACAAGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sphk2 ( NM_020011.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_064395.2 Mus musculus sphingosine kinase 2 (Sphk2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu16495
Clone ID Related Accession (Same CDS sequence)	NM_001172561.1 , XM_006541012.3 , NM_020011.5 , XM_006541012.2 , XM_006541013.2 , XM_006541013.3 , NM_203280.3 , XM_006541012.4
Accession Version	NM_020011.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1854bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-04
Organism	Mus musculus(house mouse)
Product	sphingosine kinase 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK004951.1 and AC167242.4. On Feb 20, 2010 this sequence version replaced NM_020011.4. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK004951.1, BC092084.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCCCAC CACCACTACT GCCAGTGGCT GCCAGCACTC CAATCCTGCA CGGCGAGTTT 
GGTTCCTACC CGGCCAACGG CCCACGGTTT GCCCTCACCC TCACAACACA AGCCCTACAC 
ATACAGCGAC TACGCCCAAA GCCAGAAGCC CGGCCCCGAG ATGGTCTAGT CTCTCTGGAT 
GAGGTCTCGG GCTGTGGCAC CCTGCAGAGC CGTAGCCCCG AGGACACTGC AGCCTACTTC 
TGCATCTACA CCTACCCACG TGGCCGTCGA GGGGGCCGGC GCAGAGCTAC GCGGACCTTC 
CGGGCGGATG GGGCCACCAC TTATGAGGAG AATCGTGCAG AGGCCCAGCG CTGGGCCACT 
GCCCTCACGT GTCTCCTCCG AGGAGTGCCT CTGTCAGGGG ACCAGGAAAT CACCCCTGAA 
TTGCTGCCCC GGAAGCCCAG GCTGCTCATA TTGGTCAATC CCTTTGGGGG GCGGGGCCTG 
GCCTGGCAGC GCTGTATGGA CCACGTGGTG CCAATGATCT CTGAAGCTGG GCTGTCCTTC 
AACCTCATAC AGACAGAACG ACAGAACCAT GCCCGTGAGC TGGTGCAGGG GTTAAGCCTG 
AGTGAGTGGG AAGGCATTGT CACTGTGTCT GGAGACGGGC TGCTTTACGA GGTGCTGAAT 
GGGCTCCTTG ATCGGCCAGA CTGGGAGGAT GCCGTGCGGA TGCCCATTGG TGTCCTCCCC 
TGTGGATCGG GCAATGCGCT AGCTGGGGCG GTGAACCATC ATGGCGGGTT TGAGCAGGTT 
GTCGGTGTTG ACCTGTTGCT CAACTGCTCG CTTCTTCTCT GCCGTGGTGG CAGCCATCCT 
CTGGACCTGC TCTCTGTGAC GCTAGCCTCG GGATCCCGCT GTTTTTCCTT CCTGTCAGTG 
GCCTGGGGAT TCTTGTCAGA TGTGGACATT CACAGTGAGC GCTTCAGGGC CCTGGGCAGC 
GCTCGATTCA CACTGGGTGC AGTGCTAGGC CTGGCCTCGT TGCATACCTA CCGTGGACGC 
CTCTCCTACC TCCCCGCTAC CACAGAACCA GCCTTGCCCA TCCCAGGCCA CAGTCTGCCT 
CGAGCCAAGT CAGAACTAGT CTTGGCTCCA GCCCCAGCCC CCGCCGCCAC CCACTCGCCT 
CTACATCGAT CTGTGTCTGA CCTGCCCCTG CCCCTTCCCC AGCCTGCCTT GGTCTCCCCT 
GGCTCCCCTG AGCCCCTGCC TGACCTGTCC CTCAATGGTG GTGGTCCAGA GCTGACTGGA 
GACTGGGGAG GAGCTGGGGA TGCACCTCTG TCCCCAGACC CACTGCTGCC TTCATCCCCC 
AACGCTCTCA AAACAGCTCA GCTTTCACCC ATCGCTGAAG GGCCCCCAGA AATGCCAGCA 
TCTTCGGGGT TCCTGCCTCC CACCCACAGT GCCCCAGAAG CCTCTACCTG GGGCCCAGTG 
GACCACCTCC TCCCTCCCCT GGGCTCTCCA CTGCCCCAAG ACTGGGTGAC AATAGAGGGG 
GAGTTTGTAC TCATGTTGGG CATCTTGCCG AGCCACCTCT GCGCAGACCT GATGGCAGCC 
CCACATGCAC GCTTTGATGA TGGCGTTGTG CACCTGTGTT GGGTGCGGAG CGGCATCTCA 
CGGGCTGCAC TTCTACGCAT TTTGCTGGCC ATGGAGCATG GAAACCACTT CAGCCTGGGC 
TGCCCCCATC TGGGCTATGC TGCAGCACGT GCCTTCCGCC TTGAACCACT CACGCCTCGT 
GGCCTGCTCA CTGTAGATGG GGAGTTAGTG GAGTATGGGC CAATACAGGC GCAGGTGCAC 
CCAGGTCTCG CCACGCTGCT CACTGGGCCT GCAGGTCAAA AGCCACAAGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_064395.2
CDS	675..2528
Translation	MAPPPLLPVAASTPILHGEFGSYPANGPRFALTLTTQALHIQRLRPKPEARPRDGLVSLDEVSGCGTLQSRSPEDTAAYFCIYTYPRGRRGGRRRATRTFRADGATTYEENRAEAQRWATALTCLLRGVPLSGDQEITPELLPRKPRLLILVNPFGGRGLAWQRCMDHVVPMISEAGLSFNLIQTERQNHARELVQGLSLSEWEGIVTVSGDGLLYEVLNGLLDRPDWEDAVRMPIGVLPCGSGNALAGAVNHHGGFEQVVGVDLLLNCSLLLCRGGSHPLDLLSVTLASGSRCFSFLSVAWGFLSDVDIHSERFRALGSARFTLGAVLGLASLHTYRGRLSYLPATTEPALPIPGHSLPRAKSELVLAPAPAPAATHSPLHRSVSDLPLPLPQPALVSPGSPEPLPDLSLNGGGPELTGDWGGAGDAPLSPDPLLPSSPNALKTAQLSPIAEGPPEMPASSGFLPPTHSAPEASTWGPVDHLLPPLGSPLPQDWVTIEGEFVLMLGILPSHLCADLMAAPHARFDDGVVHLCWVRSGISRAALLRILLAMEHGNHFSLGCPHLGYAAARAFRLEPLTPRGLLTVDGELVEYGPIQAQVHPGLATLLTGPAGQKPQA

Target ORF information:

RefSeq Version	NM_020011.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus sphingosine kinase 2 (Sphk2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020011.5

ORF Insert Sequence:

ATGGCCCCAC CACCACTACT GCCAGTGGCT GCCAGCACTC CAATCCTGCA CGGCGAGTTT 
GGTTCCTACC CGGCCAACGG CCCACGGTTT GCCCTCACCC TCACAACACA AGCCCTACAC 
ATACAGCGAC TACGCCCAAA GCCAGAAGCC CGGCCCCGAG ATGGTCTAGT CTCTCTGGAT 
GAGGTCTCGG GCTGTGGCAC CCTGCAGAGC CGTAGCCCCG AGGACACTGC AGCCTACTTC 
TGCATCTACA CCTACCCACG TGGCCGTCGA GGGGGCCGGC GCAGAGCTAC GCGGACCTTC 
CGGGCGGATG GGGCCACCAC TTATGAGGAG AATCGTGCAG AGGCCCAGCG CTGGGCCACT 
GCCCTCACGT GTCTCCTCCG AGGAGTGCCT CTGTCAGGGG ACCAGGAAAT CACCCCTGAA 
TTGCTGCCCC GGAAGCCCAG GCTGCTCATA TTGGTCAATC CCTTTGGGGG GCGGGGCCTG 
GCCTGGCAGC GCTGTATGGA CCACGTGGTG CCAATGATCT CTGAAGCTGG GCTGTCCTTC 
AACCTCATAC AGACAGAACG ACAGAACCAT GCCCGTGAGC TGGTGCAGGG GTTAAGCCTG 
AGTGAGTGGG AAGGCATTGT CACTGTGTCT GGAGACGGGC TGCTTTACGA GGTGCTGAAT 
GGGCTCCTTG ATCGGCCAGA CTGGGAGGAT GCCGTGCGGA TGCCCATTGG TGTCCTCCCC 
TGTGGATCGG GCAATGCGCT AGCTGGGGCG GTGAACCATC ATGGCGGGTT TGAGCAGGTT 
GTCGGTGTTG ACCTGTTGCT CAACTGCTCG CTTCTTCTCT GCCGTGGTGG CAGCCATCCT 
CTGGACCTGC TCTCTGTGAC GCTAGCCTCG GGATCCCGCT GTTTTTCCTT CCTGTCAGTG 
GCCTGGGGAT TCTTGTCAGA TGTGGACATT CACAGTGAGC GCTTCAGGGC CCTGGGCAGC 
GCTCGATTCA CACTGGGTGC AGTGCTAGGC CTGGCCTCGT TGCATACCTA CCGTGGACGC 
CTCTCCTACC TCCCCGCTAC CACAGAACCA GCCTTGCCCA TCCCAGGCCA CAGTCTGCCT 
CGAGCCAAGT CAGAACTAGT CTTGGCTCCA GCCCCAGCCC CCGCCGCCAC CCACTCGCCT 
CTACATCGAT CTGTGTCTGA CCTGCCCCTG CCCCTTCCCC AGCCTGCCTT GGTCTCCCCT 
GGCTCCCCTG AGCCCCTGCC TGACCTGTCC CTCAATGGTG GTGGTCCAGA GCTGACTGGA 
GACTGGGGAG GAGCTGGGGA TGCACCTCTG TCCCCAGACC CACTGCTGCC TTCATCCCCC 
AACGCTCTCA AAACAGCTCA GCTTTCACCC ATCGCTGAAG GGCCCCCAGA AATGCCAGCA 
TCTTCGGGGT TCCTGCCTCC CACCCACAGT GCCCCAGAAG CCTCTACCTG GGGCCCAGTG 
GACCACCTCC TCCCTCCCCT GGGCTCTCCA CTGCCCCAAG ACTGGGTGAC AATAGAGGGG 
GAGTTTGTAC TCATGTTGGG CATCTTGCCG AGCCACCTCT GCGCAGACCT GATGGCAGCC 
CCACATGCAC GCTTTGATGA TGGCGTTGTG CACCTGTGTT GGGTGCGGAG CGGCATCTCA 
CGGGCTGCAC TTCTACGCAT TTTGCTGGCC ATGGAGCATG GAAACCACTT CAGCCTGGGC 
TGCCCCCATC TGGGCTATGC TGCAGCACGT GCCTTCCGCC TTGAACCACT CACGCCTCGT 
GGCCTGCTCA CTGTAGATGG GGAGTTAGTG GAGTATGGGC CAATACAGGC GCAGGTGCAC 
CCAGGTCTCG CCACGCTGCT CACTGGGCCT GCAGGTCAAA AGCCACAAGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sphk2 ( NM_203280.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_975009.1 Mus musculus sphingosine kinase 2 (Sphk2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu16495
Clone ID Related Accession (Same CDS sequence)	NM_001172561.1 , XM_006541012.3 , NM_020011.5 , XM_006541012.2 , XM_006541013.2 , XM_006541013.3 , NM_203280.3 , XM_006541012.4
Accession Version	NM_203280.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1854bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-04
Organism	Mus musculus(house mouse)
Product	sphingosine kinase 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY256088.1, AK151990.1 and AC167242.4. On Feb 20, 2010 this sequence version replaced NM_203280.2. Transcript Variant: This variant (1) encodes the same protein as variants 2 and 3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF245448.1, BC053737.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCCCAC CACCACTACT GCCAGTGGCT GCCAGCACTC CAATCCTGCA CGGCGAGTTT 
GGTTCCTACC CGGCCAACGG CCCACGGTTT GCCCTCACCC TCACAACACA AGCCCTACAC 
ATACAGCGAC TACGCCCAAA GCCAGAAGCC CGGCCCCGAG ATGGTCTAGT CTCTCTGGAT 
GAGGTCTCGG GCTGTGGCAC CCTGCAGAGC CGTAGCCCCG AGGACACTGC AGCCTACTTC 
TGCATCTACA CCTACCCACG TGGCCGTCGA GGGGGCCGGC GCAGAGCTAC GCGGACCTTC 
CGGGCGGATG GGGCCACCAC TTATGAGGAG AATCGTGCAG AGGCCCAGCG CTGGGCCACT 
GCCCTCACGT GTCTCCTCCG AGGAGTGCCT CTGTCAGGGG ACCAGGAAAT CACCCCTGAA 
TTGCTGCCCC GGAAGCCCAG GCTGCTCATA TTGGTCAATC CCTTTGGGGG GCGGGGCCTG 
GCCTGGCAGC GCTGTATGGA CCACGTGGTG CCAATGATCT CTGAAGCTGG GCTGTCCTTC 
AACCTCATAC AGACAGAACG ACAGAACCAT GCCCGTGAGC TGGTGCAGGG GTTAAGCCTG 
AGTGAGTGGG AAGGCATTGT CACTGTGTCT GGAGACGGGC TGCTTTACGA GGTGCTGAAT 
GGGCTCCTTG ATCGGCCAGA CTGGGAGGAT GCCGTGCGGA TGCCCATTGG TGTCCTCCCC 
TGTGGATCGG GCAATGCGCT AGCTGGGGCG GTGAACCATC ATGGCGGGTT TGAGCAGGTT 
GTCGGTGTTG ACCTGTTGCT CAACTGCTCG CTTCTTCTCT GCCGTGGTGG CAGCCATCCT 
CTGGACCTGC TCTCTGTGAC GCTAGCCTCG GGATCCCGCT GTTTTTCCTT CCTGTCAGTG 
GCCTGGGGAT TCTTGTCAGA TGTGGACATT CACAGTGAGC GCTTCAGGGC CCTGGGCAGC 
GCTCGATTCA CACTGGGTGC AGTGCTAGGC CTGGCCTCGT TGCATACCTA CCGTGGACGC 
CTCTCCTACC TCCCCGCTAC CACAGAACCA GCCTTGCCCA TCCCAGGCCA CAGTCTGCCT 
CGAGCCAAGT CAGAACTAGT CTTGGCTCCA GCCCCAGCCC CCGCCGCCAC CCACTCGCCT 
CTACATCGAT CTGTGTCTGA CCTGCCCCTG CCCCTTCCCC AGCCTGCCTT GGTCTCCCCT 
GGCTCCCCTG AGCCCCTGCC TGACCTGTCC CTCAATGGTG GTGGTCCAGA GCTGACTGGA 
GACTGGGGAG GAGCTGGGGA TGCACCTCTG TCCCCAGACC CACTGCTGCC TTCATCCCCC 
AACGCTCTCA AAACAGCTCA GCTTTCACCC ATCGCTGAAG GGCCCCCAGA AATGCCAGCA 
TCTTCGGGGT TCCTGCCTCC CACCCACAGT GCCCCAGAAG CCTCTACCTG GGGCCCAGTG 
GACCACCTCC TCCCTCCCCT GGGCTCTCCA CTGCCCCAAG ACTGGGTGAC AATAGAGGGG 
GAGTTTGTAC TCATGTTGGG CATCTTGCCG AGCCACCTCT GCGCAGACCT GATGGCAGCC 
CCACATGCAC GCTTTGATGA TGGCGTTGTG CACCTGTGTT GGGTGCGGAG CGGCATCTCA 
CGGGCTGCAC TTCTACGCAT TTTGCTGGCC ATGGAGCATG GAAACCACTT CAGCCTGGGC 
TGCCCCCATC TGGGCTATGC TGCAGCACGT GCCTTCCGCC TTGAACCACT CACGCCTCGT 
GGCCTGCTCA CTGTAGATGG GGAGTTAGTG GAGTATGGGC CAATACAGGC GCAGGTGCAC 
CCAGGTCTCG CCACGCTGCT CACTGGGCCT GCAGGTCAAA AGCCACAAGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_975009.1
CDS	437..2290
Translation	MAPPPLLPVAASTPILHGEFGSYPANGPRFALTLTTQALHIQRLRPKPEARPRDGLVSLDEVSGCGTLQSRSPEDTAAYFCIYTYPRGRRGGRRRATRTFRADGATTYEENRAEAQRWATALTCLLRGVPLSGDQEITPELLPRKPRLLILVNPFGGRGLAWQRCMDHVVPMISEAGLSFNLIQTERQNHARELVQGLSLSEWEGIVTVSGDGLLYEVLNGLLDRPDWEDAVRMPIGVLPCGSGNALAGAVNHHGGFEQVVGVDLLLNCSLLLCRGGSHPLDLLSVTLASGSRCFSFLSVAWGFLSDVDIHSERFRALGSARFTLGAVLGLASLHTYRGRLSYLPATTEPALPIPGHSLPRAKSELVLAPAPAPAATHSPLHRSVSDLPLPLPQPALVSPGSPEPLPDLSLNGGGPELTGDWGGAGDAPLSPDPLLPSSPNALKTAQLSPIAEGPPEMPASSGFLPPTHSAPEASTWGPVDHLLPPLGSPLPQDWVTIEGEFVLMLGILPSHLCADLMAAPHARFDDGVVHLCWVRSGISRAALLRILLAMEHGNHFSLGCPHLGYAAARAFRLEPLTPRGLLTVDGELVEYGPIQAQVHPGLATLLTGPAGQKPQA

Target ORF information:

RefSeq Version	NM_203280.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus sphingosine kinase 2 (Sphk2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_203280.3

ORF Insert Sequence:

ATGGCCCCAC CACCACTACT GCCAGTGGCT GCCAGCACTC CAATCCTGCA CGGCGAGTTT 
GGTTCCTACC CGGCCAACGG CCCACGGTTT GCCCTCACCC TCACAACACA AGCCCTACAC 
ATACAGCGAC TACGCCCAAA GCCAGAAGCC CGGCCCCGAG ATGGTCTAGT CTCTCTGGAT 
GAGGTCTCGG GCTGTGGCAC CCTGCAGAGC CGTAGCCCCG AGGACACTGC AGCCTACTTC 
TGCATCTACA CCTACCCACG TGGCCGTCGA GGGGGCCGGC GCAGAGCTAC GCGGACCTTC 
CGGGCGGATG GGGCCACCAC TTATGAGGAG AATCGTGCAG AGGCCCAGCG CTGGGCCACT 
GCCCTCACGT GTCTCCTCCG AGGAGTGCCT CTGTCAGGGG ACCAGGAAAT CACCCCTGAA 
TTGCTGCCCC GGAAGCCCAG GCTGCTCATA TTGGTCAATC CCTTTGGGGG GCGGGGCCTG 
GCCTGGCAGC GCTGTATGGA CCACGTGGTG CCAATGATCT CTGAAGCTGG GCTGTCCTTC 
AACCTCATAC AGACAGAACG ACAGAACCAT GCCCGTGAGC TGGTGCAGGG GTTAAGCCTG 
AGTGAGTGGG AAGGCATTGT CACTGTGTCT GGAGACGGGC TGCTTTACGA GGTGCTGAAT 
GGGCTCCTTG ATCGGCCAGA CTGGGAGGAT GCCGTGCGGA TGCCCATTGG TGTCCTCCCC 
TGTGGATCGG GCAATGCGCT AGCTGGGGCG GTGAACCATC ATGGCGGGTT TGAGCAGGTT 
GTCGGTGTTG ACCTGTTGCT CAACTGCTCG CTTCTTCTCT GCCGTGGTGG CAGCCATCCT 
CTGGACCTGC TCTCTGTGAC GCTAGCCTCG GGATCCCGCT GTTTTTCCTT CCTGTCAGTG 
GCCTGGGGAT TCTTGTCAGA TGTGGACATT CACAGTGAGC GCTTCAGGGC CCTGGGCAGC 
GCTCGATTCA CACTGGGTGC AGTGCTAGGC CTGGCCTCGT TGCATACCTA CCGTGGACGC 
CTCTCCTACC TCCCCGCTAC CACAGAACCA GCCTTGCCCA TCCCAGGCCA CAGTCTGCCT 
CGAGCCAAGT CAGAACTAGT CTTGGCTCCA GCCCCAGCCC CCGCCGCCAC CCACTCGCCT 
CTACATCGAT CTGTGTCTGA CCTGCCCCTG CCCCTTCCCC AGCCTGCCTT GGTCTCCCCT 
GGCTCCCCTG AGCCCCTGCC TGACCTGTCC CTCAATGGTG GTGGTCCAGA GCTGACTGGA 
GACTGGGGAG GAGCTGGGGA TGCACCTCTG TCCCCAGACC CACTGCTGCC TTCATCCCCC 
AACGCTCTCA AAACAGCTCA GCTTTCACCC ATCGCTGAAG GGCCCCCAGA AATGCCAGCA 
TCTTCGGGGT TCCTGCCTCC CACCCACAGT GCCCCAGAAG CCTCTACCTG GGGCCCAGTG 
GACCACCTCC TCCCTCCCCT GGGCTCTCCA CTGCCCCAAG ACTGGGTGAC AATAGAGGGG 
GAGTTTGTAC TCATGTTGGG CATCTTGCCG AGCCACCTCT GCGCAGACCT GATGGCAGCC 
CCACATGCAC GCTTTGATGA TGGCGTTGTG CACCTGTGTT GGGTGCGGAG CGGCATCTCA 
CGGGCTGCAC TTCTACGCAT TTTGCTGGCC ATGGAGCATG GAAACCACTT CAGCCTGGGC 
TGCCCCCATC TGGGCTATGC TGCAGCACGT GCCTTCCGCC TTGAACCACT CACGCCTCGT 
GGCCTGCTCA CTGTAGATGG GGAGTTAGTG GAGTATGGGC CAATACAGGC GCAGGTGCAC 
CCAGGTCTCG CCACGCTGCT CACTGGGCCT GCAGGTCAAA AGCCACAAGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sphk2 ( XM_006541012.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006541075.1 Mus musculus sphingosine kinase 2 (Sphk2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu16495
Clone ID Related Accession (Same CDS sequence)	NM_001172561.1 , XM_006541012.3 , NM_020011.5 , XM_006541012.2 , XM_006541013.2 , XM_006541013.3 , NM_203280.3 , XM_006541012.4
Accession Version	XM_006541012.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1854bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	sphingosine kinase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006541012.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCAC CACCACTACT GCCAGTGGCT GCCAGCACTC CAATCCTGCA CGGCGAGTTT 
GGTTCCTACC CGGCCAACGG CCCACGGTTT GCCCTCACCC TCACAACACA AGCCCTACAC 
ATACAGCGAC TACGCCCAAA GCCAGAAGCC CGGCCCCGAG ATGGTCTAGT CTCTCTGGAT 
GAGGTCTCGG GCTGTGGCAC CCTGCAGAGC CGTAGCCCCG AGGACACTGC AGCCTACTTC 
TGCATCTACA CCTACCCACG TGGCCGTCGA GGGGGCCGGC GCAGAGCTAC GCGGACCTTC 
CGGGCGGATG GGGCCACCAC TTATGAGGAG AATCGTGCAG AGGCCCAGCG CTGGGCCACT 
GCCCTCACGT GTCTCCTCCG AGGAGTGCCT CTGTCAGGGG ACCAGGAAAT CACCCCTGAA 
TTGCTGCCCC GGAAGCCCAG GCTGCTCATA TTGGTCAATC CCTTTGGGGG GCGGGGCCTG 
GCCTGGCAGC GCTGTATGGA CCACGTGGTG CCAATGATCT CTGAAGCTGG GCTGTCCTTC 
AACCTCATAC AGACAGAACG ACAGAACCAT GCCCGTGAGC TGGTGCAGGG GTTAAGCCTG 
AGTGAGTGGG AAGGCATTGT CACTGTGTCT GGAGACGGGC TGCTTTACGA GGTGCTGAAT 
GGGCTCCTTG ATCGGCCAGA CTGGGAGGAT GCCGTGCGGA TGCCCATTGG TGTCCTCCCC 
TGTGGATCGG GCAATGCGCT AGCTGGGGCG GTGAACCATC ATGGCGGGTT TGAGCAGGTT 
GTCGGTGTTG ACCTGTTGCT CAACTGCTCG CTTCTTCTCT GCCGTGGTGG CAGCCATCCT 
CTGGACCTGC TCTCTGTGAC GCTAGCCTCG GGATCCCGCT GTTTTTCCTT CCTGTCAGTG 
GCCTGGGGAT TCTTGTCAGA TGTGGACATT CACAGTGAGC GCTTCAGGGC CCTGGGCAGC 
GCTCGATTCA CACTGGGTGC AGTGCTAGGC CTGGCCTCGT TGCATACCTA CCGTGGACGC 
CTCTCCTACC TCCCCGCTAC CACAGAACCA GCCTTGCCCA TCCCAGGCCA CAGTCTGCCT 
CGAGCCAAGT CAGAACTAGT CTTGGCTCCA GCCCCAGCCC CCGCCGCCAC CCACTCGCCT 
CTACATCGAT CTGTGTCTGA CCTGCCCCTG CCCCTTCCCC AGCCTGCCTT GGTCTCCCCT 
GGCTCCCCTG AGCCCCTGCC TGACCTGTCC CTCAATGGTG GTGGTCCAGA GCTGACTGGA 
GACTGGGGAG GAGCTGGGGA TGCACCTCTG TCCCCAGACC CACTGCTGCC TTCATCCCCC 
AACGCTCTCA AAACAGCTCA GCTTTCACCC ATCGCTGAAG GGCCCCCAGA AATGCCAGCA 
TCTTCGGGGT TCCTGCCTCC CACCCACAGT GCCCCAGAAG CCTCTACCTG GGGCCCAGTG 
GACCACCTCC TCCCTCCCCT GGGCTCTCCA CTGCCCCAAG ACTGGGTGAC AATAGAGGGG 
GAGTTTGTAC TCATGTTGGG CATCTTGCCG AGCCACCTCT GCGCAGACCT GATGGCAGCC 
CCACATGCAC GCTTTGATGA TGGCGTTGTG CACCTGTGTT GGGTGCGGAG CGGCATCTCA 
CGGGCTGCAC TTCTACGCAT TTTGCTGGCC ATGGAGCATG GAAACCACTT CAGCCTGGGC 
TGCCCCCATC TGGGCTATGC TGCAGCACGT GCCTTCCGCC TTGAACCACT CACGCCTCGT 
GGCCTGCTCA CTGTAGATGG GGAGTTAGTG GAGTATGGGC CAATACAGGC GCAGGTGCAC 
CCAGGTCTCG CCACGCTGCT CACTGGGCCT GCAGGTCAAA AGCCACAAGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006541075.1
CDS	129..1982
Translation	MAPPPLLPVAASTPILHGEFGSYPANGPRFALTLTTQALHIQRLRPKPEARPRDGLVSLDEVSGCGTLQSRSPEDTAAYFCIYTYPRGRRGGRRRATRTFRADGATTYEENRAEAQRWATALTCLLRGVPLSGDQEITPELLPRKPRLLILVNPFGGRGLAWQRCMDHVVPMISEAGLSFNLIQTERQNHARELVQGLSLSEWEGIVTVSGDGLLYEVLNGLLDRPDWEDAVRMPIGVLPCGSGNALAGAVNHHGGFEQVVGVDLLLNCSLLLCRGGSHPLDLLSVTLASGSRCFSFLSVAWGFLSDVDIHSERFRALGSARFTLGAVLGLASLHTYRGRLSYLPATTEPALPIPGHSLPRAKSELVLAPAPAPAATHSPLHRSVSDLPLPLPQPALVSPGSPEPLPDLSLNGGGPELTGDWGGAGDAPLSPDPLLPSSPNALKTAQLSPIAEGPPEMPASSGFLPPTHSAPEASTWGPVDHLLPPLGSPLPQDWVTIEGEFVLMLGILPSHLCADLMAAPHARFDDGVVHLCWVRSGISRAALLRILLAMEHGNHFSLGCPHLGYAAARAFRLEPLTPRGLLTVDGELVEYGPIQAQVHPGLATLLTGPAGQKPQA

Target ORF information:

RefSeq Version	XM_006541012.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus sphingosine kinase 2 (Sphk2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006541012.3

ORF Insert Sequence:

ATGGCCCCAC CACCACTACT GCCAGTGGCT GCCAGCACTC CAATCCTGCA CGGCGAGTTT 
GGTTCCTACC CGGCCAACGG CCCACGGTTT GCCCTCACCC TCACAACACA AGCCCTACAC 
ATACAGCGAC TACGCCCAAA GCCAGAAGCC CGGCCCCGAG ATGGTCTAGT CTCTCTGGAT 
GAGGTCTCGG GCTGTGGCAC CCTGCAGAGC CGTAGCCCCG AGGACACTGC AGCCTACTTC 
TGCATCTACA CCTACCCACG TGGCCGTCGA GGGGGCCGGC GCAGAGCTAC GCGGACCTTC 
CGGGCGGATG GGGCCACCAC TTATGAGGAG AATCGTGCAG AGGCCCAGCG CTGGGCCACT 
GCCCTCACGT GTCTCCTCCG AGGAGTGCCT CTGTCAGGGG ACCAGGAAAT CACCCCTGAA 
TTGCTGCCCC GGAAGCCCAG GCTGCTCATA TTGGTCAATC CCTTTGGGGG GCGGGGCCTG 
GCCTGGCAGC GCTGTATGGA CCACGTGGTG CCAATGATCT CTGAAGCTGG GCTGTCCTTC 
AACCTCATAC AGACAGAACG ACAGAACCAT GCCCGTGAGC TGGTGCAGGG GTTAAGCCTG 
AGTGAGTGGG AAGGCATTGT CACTGTGTCT GGAGACGGGC TGCTTTACGA GGTGCTGAAT 
GGGCTCCTTG ATCGGCCAGA CTGGGAGGAT GCCGTGCGGA TGCCCATTGG TGTCCTCCCC 
TGTGGATCGG GCAATGCGCT AGCTGGGGCG GTGAACCATC ATGGCGGGTT TGAGCAGGTT 
GTCGGTGTTG ACCTGTTGCT CAACTGCTCG CTTCTTCTCT GCCGTGGTGG CAGCCATCCT 
CTGGACCTGC TCTCTGTGAC GCTAGCCTCG GGATCCCGCT GTTTTTCCTT CCTGTCAGTG 
GCCTGGGGAT TCTTGTCAGA TGTGGACATT CACAGTGAGC GCTTCAGGGC CCTGGGCAGC 
GCTCGATTCA CACTGGGTGC AGTGCTAGGC CTGGCCTCGT TGCATACCTA CCGTGGACGC 
CTCTCCTACC TCCCCGCTAC CACAGAACCA GCCTTGCCCA TCCCAGGCCA CAGTCTGCCT 
CGAGCCAAGT CAGAACTAGT CTTGGCTCCA GCCCCAGCCC CCGCCGCCAC CCACTCGCCT 
CTACATCGAT CTGTGTCTGA CCTGCCCCTG CCCCTTCCCC AGCCTGCCTT GGTCTCCCCT 
GGCTCCCCTG AGCCCCTGCC TGACCTGTCC CTCAATGGTG GTGGTCCAGA GCTGACTGGA 
GACTGGGGAG GAGCTGGGGA TGCACCTCTG TCCCCAGACC CACTGCTGCC TTCATCCCCC 
AACGCTCTCA AAACAGCTCA GCTTTCACCC ATCGCTGAAG GGCCCCCAGA AATGCCAGCA 
TCTTCGGGGT TCCTGCCTCC CACCCACAGT GCCCCAGAAG CCTCTACCTG GGGCCCAGTG 
GACCACCTCC TCCCTCCCCT GGGCTCTCCA CTGCCCCAAG ACTGGGTGAC AATAGAGGGG 
GAGTTTGTAC TCATGTTGGG CATCTTGCCG AGCCACCTCT GCGCAGACCT GATGGCAGCC 
CCACATGCAC GCTTTGATGA TGGCGTTGTG CACCTGTGTT GGGTGCGGAG CGGCATCTCA 
CGGGCTGCAC TTCTACGCAT TTTGCTGGCC ATGGAGCATG GAAACCACTT CAGCCTGGGC 
TGCCCCCATC TGGGCTATGC TGCAGCACGT GCCTTCCGCC TTGAACCACT CACGCCTCGT 
GGCCTGCTCA CTGTAGATGG GGAGTTAGTG GAGTATGGGC CAATACAGGC GCAGGTGCAC 
CCAGGTCTCG CCACGCTGCT CACTGGGCCT GCAGGTCAAA AGCCACAAGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sphk2 ( XM_006541012.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006541075.1 Mus musculus sphingosine kinase 2 (Sphk2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu16495
Clone ID Related Accession (Same CDS sequence)	NM_001172561.1 , XM_006541012.3 , NM_020011.5 , XM_006541012.2 , XM_006541013.2 , XM_006541013.3 , NM_203280.3 , XM_006541012.4
Accession Version	XM_006541012.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1854bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	sphingosine kinase 2 isoform X1
Comment

ATGGCCCCAC CACCACTACT GCCAGTGGCT GCCAGCACTC CAATCCTGCA CGGCGAGTTT 
GGTTCCTACC CGGCCAACGG CCCACGGTTT GCCCTCACCC TCACAACACA AGCCCTACAC 
ATACAGCGAC TACGCCCAAA GCCAGAAGCC CGGCCCCGAG ATGGTCTAGT CTCTCTGGAT 
GAGGTCTCGG GCTGTGGCAC CCTGCAGAGC CGTAGCCCCG AGGACACTGC AGCCTACTTC 
TGCATCTACA CCTACCCACG TGGCCGTCGA GGGGGCCGGC GCAGAGCTAC GCGGACCTTC 
CGGGCGGATG GGGCCACCAC TTATGAGGAG AATCGTGCAG AGGCCCAGCG CTGGGCCACT 
GCCCTCACGT GTCTCCTCCG AGGAGTGCCT CTGTCAGGGG ACCAGGAAAT CACCCCTGAA 
TTGCTGCCCC GGAAGCCCAG GCTGCTCATA TTGGTCAATC CCTTTGGGGG GCGGGGCCTG 
GCCTGGCAGC GCTGTATGGA CCACGTGGTG CCAATGATCT CTGAAGCTGG GCTGTCCTTC 
AACCTCATAC AGACAGAACG ACAGAACCAT GCCCGTGAGC TGGTGCAGGG GTTAAGCCTG 
AGTGAGTGGG AAGGCATTGT CACTGTGTCT GGAGACGGGC TGCTTTACGA GGTGCTGAAT 
GGGCTCCTTG ATCGGCCAGA CTGGGAGGAT GCCGTGCGGA TGCCCATTGG TGTCCTCCCC 
TGTGGATCGG GCAATGCGCT AGCTGGGGCG GTGAACCATC ATGGCGGGTT TGAGCAGGTT 
GTCGGTGTTG ACCTGTTGCT CAACTGCTCG CTTCTTCTCT GCCGTGGTGG CAGCCATCCT 
CTGGACCTGC TCTCTGTGAC GCTAGCCTCG GGATCCCGCT GTTTTTCCTT CCTGTCAGTG 
GCCTGGGGAT TCTTGTCAGA TGTGGACATT CACAGTGAGC GCTTCAGGGC CCTGGGCAGC 
GCTCGATTCA CACTGGGTGC AGTGCTAGGC CTGGCCTCGT TGCATACCTA CCGTGGACGC 
CTCTCCTACC TCCCCGCTAC CACAGAACCA GCCTTGCCCA TCCCAGGCCA CAGTCTGCCT 
CGAGCCAAGT CAGAACTAGT CTTGGCTCCA GCCCCAGCCC CCGCCGCCAC CCACTCGCCT 
CTACATCGAT CTGTGTCTGA CCTGCCCCTG CCCCTTCCCC AGCCTGCCTT GGTCTCCCCT 
GGCTCCCCTG AGCCCCTGCC TGACCTGTCC CTCAATGGTG GTGGTCCAGA GCTGACTGGA 
GACTGGGGAG GAGCTGGGGA TGCACCTCTG TCCCCAGACC CACTGCTGCC TTCATCCCCC 
AACGCTCTCA AAACAGCTCA GCTTTCACCC ATCGCTGAAG GGCCCCCAGA AATGCCAGCA 
TCTTCGGGGT TCCTGCCTCC CACCCACAGT GCCCCAGAAG CCTCTACCTG GGGCCCAGTG 
GACCACCTCC TCCCTCCCCT GGGCTCTCCA CTGCCCCAAG ACTGGGTGAC AATAGAGGGG 
GAGTTTGTAC TCATGTTGGG CATCTTGCCG AGCCACCTCT GCGCAGACCT GATGGCAGCC 
CCACATGCAC GCTTTGATGA TGGCGTTGTG CACCTGTGTT GGGTGCGGAG CGGCATCTCA 
CGGGCTGCAC TTCTACGCAT TTTGCTGGCC ATGGAGCATG GAAACCACTT CAGCCTGGGC 
TGCCCCCATC TGGGCTATGC TGCAGCACGT GCCTTCCGCC TTGAACCACT CACGCCTCGT 
GGCCTGCTCA CTGTAGATGG GGAGTTAGTG GAGTATGGGC CAATACAGGC GCAGGTGCAC 
CCAGGTCTCG CCACGCTGCT CACTGGGCCT GCAGGTCAAA AGCCACAAGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_006541075.1

Translation

MAPPPLLPVAASTPILHGEFGSYPANGPRFALTLTTQALHIQRLRPKPEARPRDGLVSLDEVSGCGTLQSRSPEDTAAYFCIYTYPRGRRGGRRRATRTFRADGATTYEENRAEAQRWATALTCLLRGVPLSGDQEITPELLPRKPRLLILVNPFGGRGLAWQRCMDHVVPMISEAGLSFNLIQTERQNHARELVQGLSLSEWEGIVTVSGDGLLYEVLNGLLDRPDWEDAVRMPIGVLPCGSGNALAGAVNHHGGFEQVVGVDLLLNCSLLLCRGGSHPLDLLSVTLASGSRCFSFLSVAWGFLSDVDIHSERFRALGSARFTLGAVLGLASLHTYRGRLSYLPATTEPALPIPGHSLPRAKSELVLAPAPAPAATHSPLHRSVSDLPLPLPQPALVSPGSPEPLPDLSLNGGGPELTGDWGGAGDAPLSPDPLLPSSPNALKTAQLSPIAEGPPEMPASSGFLPPTHSAPEASTWGPVDHLLPPLGSPLPQDWVTIEGEFVLMLGILPSHLCADLMAAPHARFDDGVVHLCWVRSGISRAALLRILLAMEHGNHFSLGCPHLGYAAARAFRLEPLTPRGLLTVDGELVEYGPIQAQVHPGLATLLTGPAGQKPQA

Target ORF information:

RefSeq Version	XM_006541012.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus sphingosine kinase 2 (Sphk2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006541012.2

ORF Insert Sequence:

ATGGCCCCAC CACCACTACT GCCAGTGGCT GCCAGCACTC CAATCCTGCA CGGCGAGTTT 
GGTTCCTACC CGGCCAACGG CCCACGGTTT GCCCTCACCC TCACAACACA AGCCCTACAC 
ATACAGCGAC TACGCCCAAA GCCAGAAGCC CGGCCCCGAG ATGGTCTAGT CTCTCTGGAT 
GAGGTCTCGG GCTGTGGCAC CCTGCAGAGC CGTAGCCCCG AGGACACTGC AGCCTACTTC 
TGCATCTACA CCTACCCACG TGGCCGTCGA GGGGGCCGGC GCAGAGCTAC GCGGACCTTC 
CGGGCGGATG GGGCCACCAC TTATGAGGAG AATCGTGCAG AGGCCCAGCG CTGGGCCACT 
GCCCTCACGT GTCTCCTCCG AGGAGTGCCT CTGTCAGGGG ACCAGGAAAT CACCCCTGAA 
TTGCTGCCCC GGAAGCCCAG GCTGCTCATA TTGGTCAATC CCTTTGGGGG GCGGGGCCTG 
GCCTGGCAGC GCTGTATGGA CCACGTGGTG CCAATGATCT CTGAAGCTGG GCTGTCCTTC 
AACCTCATAC AGACAGAACG ACAGAACCAT GCCCGTGAGC TGGTGCAGGG GTTAAGCCTG 
AGTGAGTGGG AAGGCATTGT CACTGTGTCT GGAGACGGGC TGCTTTACGA GGTGCTGAAT 
GGGCTCCTTG ATCGGCCAGA CTGGGAGGAT GCCGTGCGGA TGCCCATTGG TGTCCTCCCC 
TGTGGATCGG GCAATGCGCT AGCTGGGGCG GTGAACCATC ATGGCGGGTT TGAGCAGGTT 
GTCGGTGTTG ACCTGTTGCT CAACTGCTCG CTTCTTCTCT GCCGTGGTGG CAGCCATCCT 
CTGGACCTGC TCTCTGTGAC GCTAGCCTCG GGATCCCGCT GTTTTTCCTT CCTGTCAGTG 
GCCTGGGGAT TCTTGTCAGA TGTGGACATT CACAGTGAGC GCTTCAGGGC CCTGGGCAGC 
GCTCGATTCA CACTGGGTGC AGTGCTAGGC CTGGCCTCGT TGCATACCTA CCGTGGACGC 
CTCTCCTACC TCCCCGCTAC CACAGAACCA GCCTTGCCCA TCCCAGGCCA CAGTCTGCCT 
CGAGCCAAGT CAGAACTAGT CTTGGCTCCA GCCCCAGCCC CCGCCGCCAC CCACTCGCCT 
CTACATCGAT CTGTGTCTGA CCTGCCCCTG CCCCTTCCCC AGCCTGCCTT GGTCTCCCCT 
GGCTCCCCTG AGCCCCTGCC TGACCTGTCC CTCAATGGTG GTGGTCCAGA GCTGACTGGA 
GACTGGGGAG GAGCTGGGGA TGCACCTCTG TCCCCAGACC CACTGCTGCC TTCATCCCCC 
AACGCTCTCA AAACAGCTCA GCTTTCACCC ATCGCTGAAG GGCCCCCAGA AATGCCAGCA 
TCTTCGGGGT TCCTGCCTCC CACCCACAGT GCCCCAGAAG CCTCTACCTG GGGCCCAGTG 
GACCACCTCC TCCCTCCCCT GGGCTCTCCA CTGCCCCAAG ACTGGGTGAC AATAGAGGGG 
GAGTTTGTAC TCATGTTGGG CATCTTGCCG AGCCACCTCT GCGCAGACCT GATGGCAGCC 
CCACATGCAC GCTTTGATGA TGGCGTTGTG CACCTGTGTT GGGTGCGGAG CGGCATCTCA 
CGGGCTGCAC TTCTACGCAT TTTGCTGGCC ATGGAGCATG GAAACCACTT CAGCCTGGGC 
TGCCCCCATC TGGGCTATGC TGCAGCACGT GCCTTCCGCC TTGAACCACT CACGCCTCGT 
GGCCTGCTCA CTGTAGATGG GGAGTTAGTG GAGTATGGGC CAATACAGGC GCAGGTGCAC 
CCAGGTCTCG CCACGCTGCT CACTGGGCCT GCAGGTCAAA AGCCACAAGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sphk2 ( XM_006541013.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006541076.1 Mus musculus sphingosine kinase 2 (Sphk2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu16495
Clone ID Related Accession (Same CDS sequence)	NM_001172561.1 , XM_006541012.3 , NM_020011.5 , XM_006541012.2 , XM_006541013.2 , XM_006541013.3 , NM_203280.3 , XM_006541012.4
Accession Version	XM_006541013.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1854bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	sphingosine kinase 2 isoform X1
Comment

ATGGCCCCAC CACCACTACT GCCAGTGGCT GCCAGCACTC CAATCCTGCA CGGCGAGTTT 
GGTTCCTACC CGGCCAACGG CCCACGGTTT GCCCTCACCC TCACAACACA AGCCCTACAC 
ATACAGCGAC TACGCCCAAA GCCAGAAGCC CGGCCCCGAG ATGGTCTAGT CTCTCTGGAT 
GAGGTCTCGG GCTGTGGCAC CCTGCAGAGC CGTAGCCCCG AGGACACTGC AGCCTACTTC 
TGCATCTACA CCTACCCACG TGGCCGTCGA GGGGGCCGGC GCAGAGCTAC GCGGACCTTC 
CGGGCGGATG GGGCCACCAC TTATGAGGAG AATCGTGCAG AGGCCCAGCG CTGGGCCACT 
GCCCTCACGT GTCTCCTCCG AGGAGTGCCT CTGTCAGGGG ACCAGGAAAT CACCCCTGAA 
TTGCTGCCCC GGAAGCCCAG GCTGCTCATA TTGGTCAATC CCTTTGGGGG GCGGGGCCTG 
GCCTGGCAGC GCTGTATGGA CCACGTGGTG CCAATGATCT CTGAAGCTGG GCTGTCCTTC 
AACCTCATAC AGACAGAACG ACAGAACCAT GCCCGTGAGC TGGTGCAGGG GTTAAGCCTG 
AGTGAGTGGG AAGGCATTGT CACTGTGTCT GGAGACGGGC TGCTTTACGA GGTGCTGAAT 
GGGCTCCTTG ATCGGCCAGA CTGGGAGGAT GCCGTGCGGA TGCCCATTGG TGTCCTCCCC 
TGTGGATCGG GCAATGCGCT AGCTGGGGCG GTGAACCATC ATGGCGGGTT TGAGCAGGTT 
GTCGGTGTTG ACCTGTTGCT CAACTGCTCG CTTCTTCTCT GCCGTGGTGG CAGCCATCCT 
CTGGACCTGC TCTCTGTGAC GCTAGCCTCG GGATCCCGCT GTTTTTCCTT CCTGTCAGTG 
GCCTGGGGAT TCTTGTCAGA TGTGGACATT CACAGTGAGC GCTTCAGGGC CCTGGGCAGC 
GCTCGATTCA CACTGGGTGC AGTGCTAGGC CTGGCCTCGT TGCATACCTA CCGTGGACGC 
CTCTCCTACC TCCCCGCTAC CACAGAACCA GCCTTGCCCA TCCCAGGCCA CAGTCTGCCT 
CGAGCCAAGT CAGAACTAGT CTTGGCTCCA GCCCCAGCCC CCGCCGCCAC CCACTCGCCT 
CTACATCGAT CTGTGTCTGA CCTGCCCCTG CCCCTTCCCC AGCCTGCCTT GGTCTCCCCT 
GGCTCCCCTG AGCCCCTGCC TGACCTGTCC CTCAATGGTG GTGGTCCAGA GCTGACTGGA 
GACTGGGGAG GAGCTGGGGA TGCACCTCTG TCCCCAGACC CACTGCTGCC TTCATCCCCC 
AACGCTCTCA AAACAGCTCA GCTTTCACCC ATCGCTGAAG GGCCCCCAGA AATGCCAGCA 
TCTTCGGGGT TCCTGCCTCC CACCCACAGT GCCCCAGAAG CCTCTACCTG GGGCCCAGTG 
GACCACCTCC TCCCTCCCCT GGGCTCTCCA CTGCCCCAAG ACTGGGTGAC AATAGAGGGG 
GAGTTTGTAC TCATGTTGGG CATCTTGCCG AGCCACCTCT GCGCAGACCT GATGGCAGCC 
CCACATGCAC GCTTTGATGA TGGCGTTGTG CACCTGTGTT GGGTGCGGAG CGGCATCTCA 
CGGGCTGCAC TTCTACGCAT TTTGCTGGCC ATGGAGCATG GAAACCACTT CAGCCTGGGC 
TGCCCCCATC TGGGCTATGC TGCAGCACGT GCCTTCCGCC TTGAACCACT CACGCCTCGT 
GGCCTGCTCA CTGTAGATGG GGAGTTAGTG GAGTATGGGC CAATACAGGC GCAGGTGCAC 
CCAGGTCTCG CCACGCTGCT CACTGGGCCT GCAGGTCAAA AGCCACAAGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_006541076.1

Translation

MAPPPLLPVAASTPILHGEFGSYPANGPRFALTLTTQALHIQRLRPKPEARPRDGLVSLDEVSGCGTLQSRSPEDTAAYFCIYTYPRGRRGGRRRATRTFRADGATTYEENRAEAQRWATALTCLLRGVPLSGDQEITPELLPRKPRLLILVNPFGGRGLAWQRCMDHVVPMISEAGLSFNLIQTERQNHARELVQGLSLSEWEGIVTVSGDGLLYEVLNGLLDRPDWEDAVRMPIGVLPCGSGNALAGAVNHHGGFEQVVGVDLLLNCSLLLCRGGSHPLDLLSVTLASGSRCFSFLSVAWGFLSDVDIHSERFRALGSARFTLGAVLGLASLHTYRGRLSYLPATTEPALPIPGHSLPRAKSELVLAPAPAPAATHSPLHRSVSDLPLPLPQPALVSPGSPEPLPDLSLNGGGPELTGDWGGAGDAPLSPDPLLPSSPNALKTAQLSPIAEGPPEMPASSGFLPPTHSAPEASTWGPVDHLLPPLGSPLPQDWVTIEGEFVLMLGILPSHLCADLMAAPHARFDDGVVHLCWVRSGISRAALLRILLAMEHGNHFSLGCPHLGYAAARAFRLEPLTPRGLLTVDGELVEYGPIQAQVHPGLATLLTGPAGQKPQA

Target ORF information:

RefSeq Version	XM_006541013.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus sphingosine kinase 2 (Sphk2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006541013.2

ORF Insert Sequence:

ATGGCCCCAC CACCACTACT GCCAGTGGCT GCCAGCACTC CAATCCTGCA CGGCGAGTTT 
GGTTCCTACC CGGCCAACGG CCCACGGTTT GCCCTCACCC TCACAACACA AGCCCTACAC 
ATACAGCGAC TACGCCCAAA GCCAGAAGCC CGGCCCCGAG ATGGTCTAGT CTCTCTGGAT 
GAGGTCTCGG GCTGTGGCAC CCTGCAGAGC CGTAGCCCCG AGGACACTGC AGCCTACTTC 
TGCATCTACA CCTACCCACG TGGCCGTCGA GGGGGCCGGC GCAGAGCTAC GCGGACCTTC 
CGGGCGGATG GGGCCACCAC TTATGAGGAG AATCGTGCAG AGGCCCAGCG CTGGGCCACT 
GCCCTCACGT GTCTCCTCCG AGGAGTGCCT CTGTCAGGGG ACCAGGAAAT CACCCCTGAA 
TTGCTGCCCC GGAAGCCCAG GCTGCTCATA TTGGTCAATC CCTTTGGGGG GCGGGGCCTG 
GCCTGGCAGC GCTGTATGGA CCACGTGGTG CCAATGATCT CTGAAGCTGG GCTGTCCTTC 
AACCTCATAC AGACAGAACG ACAGAACCAT GCCCGTGAGC TGGTGCAGGG GTTAAGCCTG 
AGTGAGTGGG AAGGCATTGT CACTGTGTCT GGAGACGGGC TGCTTTACGA GGTGCTGAAT 
GGGCTCCTTG ATCGGCCAGA CTGGGAGGAT GCCGTGCGGA TGCCCATTGG TGTCCTCCCC 
TGTGGATCGG GCAATGCGCT AGCTGGGGCG GTGAACCATC ATGGCGGGTT TGAGCAGGTT 
GTCGGTGTTG ACCTGTTGCT CAACTGCTCG CTTCTTCTCT GCCGTGGTGG CAGCCATCCT 
CTGGACCTGC TCTCTGTGAC GCTAGCCTCG GGATCCCGCT GTTTTTCCTT CCTGTCAGTG 
GCCTGGGGAT TCTTGTCAGA TGTGGACATT CACAGTGAGC GCTTCAGGGC CCTGGGCAGC 
GCTCGATTCA CACTGGGTGC AGTGCTAGGC CTGGCCTCGT TGCATACCTA CCGTGGACGC 
CTCTCCTACC TCCCCGCTAC CACAGAACCA GCCTTGCCCA TCCCAGGCCA CAGTCTGCCT 
CGAGCCAAGT CAGAACTAGT CTTGGCTCCA GCCCCAGCCC CCGCCGCCAC CCACTCGCCT 
CTACATCGAT CTGTGTCTGA CCTGCCCCTG CCCCTTCCCC AGCCTGCCTT GGTCTCCCCT 
GGCTCCCCTG AGCCCCTGCC TGACCTGTCC CTCAATGGTG GTGGTCCAGA GCTGACTGGA 
GACTGGGGAG GAGCTGGGGA TGCACCTCTG TCCCCAGACC CACTGCTGCC TTCATCCCCC 
AACGCTCTCA AAACAGCTCA GCTTTCACCC ATCGCTGAAG GGCCCCCAGA AATGCCAGCA 
TCTTCGGGGT TCCTGCCTCC CACCCACAGT GCCCCAGAAG CCTCTACCTG GGGCCCAGTG 
GACCACCTCC TCCCTCCCCT GGGCTCTCCA CTGCCCCAAG ACTGGGTGAC AATAGAGGGG 
GAGTTTGTAC TCATGTTGGG CATCTTGCCG AGCCACCTCT GCGCAGACCT GATGGCAGCC 
CCACATGCAC GCTTTGATGA TGGCGTTGTG CACCTGTGTT GGGTGCGGAG CGGCATCTCA 
CGGGCTGCAC TTCTACGCAT TTTGCTGGCC ATGGAGCATG GAAACCACTT CAGCCTGGGC 
TGCCCCCATC TGGGCTATGC TGCAGCACGT GCCTTCCGCC TTGAACCACT CACGCCTCGT 
GGCCTGCTCA CTGTAGATGG GGAGTTAGTG GAGTATGGGC CAATACAGGC GCAGGTGCAC 
CCAGGTCTCG CCACGCTGCT CACTGGGCCT GCAGGTCAAA AGCCACAAGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sphk2 ( XM_006541013.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006541076.1 Mus musculus sphingosine kinase 2 (Sphk2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu16495
Clone ID Related Accession (Same CDS sequence)	NM_001172561.1 , XM_006541012.3 , NM_020011.5 , XM_006541012.2 , XM_006541013.2 , XM_006541013.3 , NM_203280.3 , XM_006541012.4
Accession Version	XM_006541013.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1854bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	sphingosine kinase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006541013.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCAC CACCACTACT GCCAGTGGCT GCCAGCACTC CAATCCTGCA CGGCGAGTTT 
GGTTCCTACC CGGCCAACGG CCCACGGTTT GCCCTCACCC TCACAACACA AGCCCTACAC 
ATACAGCGAC TACGCCCAAA GCCAGAAGCC CGGCCCCGAG ATGGTCTAGT CTCTCTGGAT 
GAGGTCTCGG GCTGTGGCAC CCTGCAGAGC CGTAGCCCCG AGGACACTGC AGCCTACTTC 
TGCATCTACA CCTACCCACG TGGCCGTCGA GGGGGCCGGC GCAGAGCTAC GCGGACCTTC 
CGGGCGGATG GGGCCACCAC TTATGAGGAG AATCGTGCAG AGGCCCAGCG CTGGGCCACT 
GCCCTCACGT GTCTCCTCCG AGGAGTGCCT CTGTCAGGGG ACCAGGAAAT CACCCCTGAA 
TTGCTGCCCC GGAAGCCCAG GCTGCTCATA TTGGTCAATC CCTTTGGGGG GCGGGGCCTG 
GCCTGGCAGC GCTGTATGGA CCACGTGGTG CCAATGATCT CTGAAGCTGG GCTGTCCTTC 
AACCTCATAC AGACAGAACG ACAGAACCAT GCCCGTGAGC TGGTGCAGGG GTTAAGCCTG 
AGTGAGTGGG AAGGCATTGT CACTGTGTCT GGAGACGGGC TGCTTTACGA GGTGCTGAAT 
GGGCTCCTTG ATCGGCCAGA CTGGGAGGAT GCCGTGCGGA TGCCCATTGG TGTCCTCCCC 
TGTGGATCGG GCAATGCGCT AGCTGGGGCG GTGAACCATC ATGGCGGGTT TGAGCAGGTT 
GTCGGTGTTG ACCTGTTGCT CAACTGCTCG CTTCTTCTCT GCCGTGGTGG CAGCCATCCT 
CTGGACCTGC TCTCTGTGAC GCTAGCCTCG GGATCCCGCT GTTTTTCCTT CCTGTCAGTG 
GCCTGGGGAT TCTTGTCAGA TGTGGACATT CACAGTGAGC GCTTCAGGGC CCTGGGCAGC 
GCTCGATTCA CACTGGGTGC AGTGCTAGGC CTGGCCTCGT TGCATACCTA CCGTGGACGC 
CTCTCCTACC TCCCCGCTAC CACAGAACCA GCCTTGCCCA TCCCAGGCCA CAGTCTGCCT 
CGAGCCAAGT CAGAACTAGT CTTGGCTCCA GCCCCAGCCC CCGCCGCCAC CCACTCGCCT 
CTACATCGAT CTGTGTCTGA CCTGCCCCTG CCCCTTCCCC AGCCTGCCTT GGTCTCCCCT 
GGCTCCCCTG AGCCCCTGCC TGACCTGTCC CTCAATGGTG GTGGTCCAGA GCTGACTGGA 
GACTGGGGAG GAGCTGGGGA TGCACCTCTG TCCCCAGACC CACTGCTGCC TTCATCCCCC 
AACGCTCTCA AAACAGCTCA GCTTTCACCC ATCGCTGAAG GGCCCCCAGA AATGCCAGCA 
TCTTCGGGGT TCCTGCCTCC CACCCACAGT GCCCCAGAAG CCTCTACCTG GGGCCCAGTG 
GACCACCTCC TCCCTCCCCT GGGCTCTCCA CTGCCCCAAG ACTGGGTGAC AATAGAGGGG 
GAGTTTGTAC TCATGTTGGG CATCTTGCCG AGCCACCTCT GCGCAGACCT GATGGCAGCC 
CCACATGCAC GCTTTGATGA TGGCGTTGTG CACCTGTGTT GGGTGCGGAG CGGCATCTCA 
CGGGCTGCAC TTCTACGCAT TTTGCTGGCC ATGGAGCATG GAAACCACTT CAGCCTGGGC 
TGCCCCCATC TGGGCTATGC TGCAGCACGT GCCTTCCGCC TTGAACCACT CACGCCTCGT 
GGCCTGCTCA CTGTAGATGG GGAGTTAGTG GAGTATGGGC CAATACAGGC GCAGGTGCAC 
CCAGGTCTCG CCACGCTGCT CACTGGGCCT GCAGGTCAAA AGCCACAAGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006541076.1
CDS	88..1941
Translation	MAPPPLLPVAASTPILHGEFGSYPANGPRFALTLTTQALHIQRLRPKPEARPRDGLVSLDEVSGCGTLQSRSPEDTAAYFCIYTYPRGRRGGRRRATRTFRADGATTYEENRAEAQRWATALTCLLRGVPLSGDQEITPELLPRKPRLLILVNPFGGRGLAWQRCMDHVVPMISEAGLSFNLIQTERQNHARELVQGLSLSEWEGIVTVSGDGLLYEVLNGLLDRPDWEDAVRMPIGVLPCGSGNALAGAVNHHGGFEQVVGVDLLLNCSLLLCRGGSHPLDLLSVTLASGSRCFSFLSVAWGFLSDVDIHSERFRALGSARFTLGAVLGLASLHTYRGRLSYLPATTEPALPIPGHSLPRAKSELVLAPAPAPAATHSPLHRSVSDLPLPLPQPALVSPGSPEPLPDLSLNGGGPELTGDWGGAGDAPLSPDPLLPSSPNALKTAQLSPIAEGPPEMPASSGFLPPTHSAPEASTWGPVDHLLPPLGSPLPQDWVTIEGEFVLMLGILPSHLCADLMAAPHARFDDGVVHLCWVRSGISRAALLRILLAMEHGNHFSLGCPHLGYAAARAFRLEPLTPRGLLTVDGELVEYGPIQAQVHPGLATLLTGPAGQKPQA

Target ORF information:

RefSeq Version	XM_006541013.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus sphingosine kinase 2 (Sphk2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006541013.3

ORF Insert Sequence:

ATGGCCCCAC CACCACTACT GCCAGTGGCT GCCAGCACTC CAATCCTGCA CGGCGAGTTT 
GGTTCCTACC CGGCCAACGG CCCACGGTTT GCCCTCACCC TCACAACACA AGCCCTACAC 
ATACAGCGAC TACGCCCAAA GCCAGAAGCC CGGCCCCGAG ATGGTCTAGT CTCTCTGGAT 
GAGGTCTCGG GCTGTGGCAC CCTGCAGAGC CGTAGCCCCG AGGACACTGC AGCCTACTTC 
TGCATCTACA CCTACCCACG TGGCCGTCGA GGGGGCCGGC GCAGAGCTAC GCGGACCTTC 
CGGGCGGATG GGGCCACCAC TTATGAGGAG AATCGTGCAG AGGCCCAGCG CTGGGCCACT 
GCCCTCACGT GTCTCCTCCG AGGAGTGCCT CTGTCAGGGG ACCAGGAAAT CACCCCTGAA 
TTGCTGCCCC GGAAGCCCAG GCTGCTCATA TTGGTCAATC CCTTTGGGGG GCGGGGCCTG 
GCCTGGCAGC GCTGTATGGA CCACGTGGTG CCAATGATCT CTGAAGCTGG GCTGTCCTTC 
AACCTCATAC AGACAGAACG ACAGAACCAT GCCCGTGAGC TGGTGCAGGG GTTAAGCCTG 
AGTGAGTGGG AAGGCATTGT CACTGTGTCT GGAGACGGGC TGCTTTACGA GGTGCTGAAT 
GGGCTCCTTG ATCGGCCAGA CTGGGAGGAT GCCGTGCGGA TGCCCATTGG TGTCCTCCCC 
TGTGGATCGG GCAATGCGCT AGCTGGGGCG GTGAACCATC ATGGCGGGTT TGAGCAGGTT 
GTCGGTGTTG ACCTGTTGCT CAACTGCTCG CTTCTTCTCT GCCGTGGTGG CAGCCATCCT 
CTGGACCTGC TCTCTGTGAC GCTAGCCTCG GGATCCCGCT GTTTTTCCTT CCTGTCAGTG 
GCCTGGGGAT TCTTGTCAGA TGTGGACATT CACAGTGAGC GCTTCAGGGC CCTGGGCAGC 
GCTCGATTCA CACTGGGTGC AGTGCTAGGC CTGGCCTCGT TGCATACCTA CCGTGGACGC 
CTCTCCTACC TCCCCGCTAC CACAGAACCA GCCTTGCCCA TCCCAGGCCA CAGTCTGCCT 
CGAGCCAAGT CAGAACTAGT CTTGGCTCCA GCCCCAGCCC CCGCCGCCAC CCACTCGCCT 
CTACATCGAT CTGTGTCTGA CCTGCCCCTG CCCCTTCCCC AGCCTGCCTT GGTCTCCCCT 
GGCTCCCCTG AGCCCCTGCC TGACCTGTCC CTCAATGGTG GTGGTCCAGA GCTGACTGGA 
GACTGGGGAG GAGCTGGGGA TGCACCTCTG TCCCCAGACC CACTGCTGCC TTCATCCCCC 
AACGCTCTCA AAACAGCTCA GCTTTCACCC ATCGCTGAAG GGCCCCCAGA AATGCCAGCA 
TCTTCGGGGT TCCTGCCTCC CACCCACAGT GCCCCAGAAG CCTCTACCTG GGGCCCAGTG 
GACCACCTCC TCCCTCCCCT GGGCTCTCCA CTGCCCCAAG ACTGGGTGAC AATAGAGGGG 
GAGTTTGTAC TCATGTTGGG CATCTTGCCG AGCCACCTCT GCGCAGACCT GATGGCAGCC 
CCACATGCAC GCTTTGATGA TGGCGTTGTG CACCTGTGTT GGGTGCGGAG CGGCATCTCA 
CGGGCTGCAC TTCTACGCAT TTTGCTGGCC ATGGAGCATG GAAACCACTT CAGCCTGGGC 
TGCCCCCATC TGGGCTATGC TGCAGCACGT GCCTTCCGCC TTGAACCACT CACGCCTCGT 
GGCCTGCTCA CTGTAGATGG GGAGTTAGTG GAGTATGGGC CAATACAGGC GCAGGTGCAC 
CCAGGTCTCG CCACGCTGCT CACTGGGCCT GCAGGTCAAA AGCCACAAGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sphk2 ( XM_006541012.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006541075.1 Mus musculus sphingosine kinase 2 (Sphk2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu16495
Clone ID Related Accession (Same CDS sequence)	NM_001172561.1 , XM_006541012.3 , NM_020011.5 , XM_006541012.2 , XM_006541013.2 , XM_006541013.3 , NM_203280.3 , XM_006541012.4
Accession Version	XM_006541012.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1854bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	sphingosine kinase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006541012.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCAC CACCACTACT GCCAGTGGCT GCCAGCACTC CAATCCTGCA CGGCGAGTTT 
GGTTCCTACC CGGCCAACGG CCCACGGTTT GCCCTCACCC TCACAACACA AGCCCTACAC 
ATACAGCGAC TACGCCCAAA GCCAGAAGCC CGGCCCCGAG ATGGTCTAGT CTCTCTGGAT 
GAGGTCTCGG GCTGTGGCAC CCTGCAGAGC CGTAGCCCCG AGGACACTGC AGCCTACTTC 
TGCATCTACA CCTACCCACG TGGCCGTCGA GGGGGCCGGC GCAGAGCTAC GCGGACCTTC 
CGGGCGGATG GGGCCACCAC TTATGAGGAG AATCGTGCAG AGGCCCAGCG CTGGGCCACT 
GCCCTCACGT GTCTCCTCCG AGGAGTGCCT CTGTCAGGGG ACCAGGAAAT CACCCCTGAA 
TTGCTGCCCC GGAAGCCCAG GCTGCTCATA TTGGTCAATC CCTTTGGGGG GCGGGGCCTG 
GCCTGGCAGC GCTGTATGGA CCACGTGGTG CCAATGATCT CTGAAGCTGG GCTGTCCTTC 
AACCTCATAC AGACAGAACG ACAGAACCAT GCCCGTGAGC TGGTGCAGGG GTTAAGCCTG 
AGTGAGTGGG AAGGCATTGT CACTGTGTCT GGAGACGGGC TGCTTTACGA GGTGCTGAAT 
GGGCTCCTTG ATCGGCCAGA CTGGGAGGAT GCCGTGCGGA TGCCCATTGG TGTCCTCCCC 
TGTGGATCGG GCAATGCGCT AGCTGGGGCG GTGAACCATC ATGGCGGGTT TGAGCAGGTT 
GTCGGTGTTG ACCTGTTGCT CAACTGCTCG CTTCTTCTCT GCCGTGGTGG CAGCCATCCT 
CTGGACCTGC TCTCTGTGAC GCTAGCCTCG GGATCCCGCT GTTTTTCCTT CCTGTCAGTG 
GCCTGGGGAT TCTTGTCAGA TGTGGACATT CACAGTGAGC GCTTCAGGGC CCTGGGCAGC 
GCTCGATTCA CACTGGGTGC AGTGCTAGGC CTGGCCTCGT TGCATACCTA CCGTGGACGC 
CTCTCCTACC TCCCCGCTAC CACAGAACCA GCCTTGCCCA TCCCAGGCCA CAGTCTGCCT 
CGAGCCAAGT CAGAACTAGT CTTGGCTCCA GCCCCAGCCC CCGCCGCCAC CCACTCGCCT 
CTACATCGAT CTGTGTCTGA CCTGCCCCTG CCCCTTCCCC AGCCTGCCTT GGTCTCCCCT 
GGCTCCCCTG AGCCCCTGCC TGACCTGTCC CTCAATGGTG GTGGTCCAGA GCTGACTGGA 
GACTGGGGAG GAGCTGGGGA TGCACCTCTG TCCCCAGACC CACTGCTGCC TTCATCCCCC 
AACGCTCTCA AAACAGCTCA GCTTTCACCC ATCGCTGAAG GGCCCCCAGA AATGCCAGCA 
TCTTCGGGGT TCCTGCCTCC CACCCACAGT GCCCCAGAAG CCTCTACCTG GGGCCCAGTG 
GACCACCTCC TCCCTCCCCT GGGCTCTCCA CTGCCCCAAG ACTGGGTGAC AATAGAGGGG 
GAGTTTGTAC TCATGTTGGG CATCTTGCCG AGCCACCTCT GCGCAGACCT GATGGCAGCC 
CCACATGCAC GCTTTGATGA TGGCGTTGTG CACCTGTGTT GGGTGCGGAG CGGCATCTCA 
CGGGCTGCAC TTCTACGCAT TTTGCTGGCC ATGGAGCATG GAAACCACTT CAGCCTGGGC 
TGCCCCCATC TGGGCTATGC TGCAGCACGT GCCTTCCGCC TTGAACCACT CACGCCTCGT 
GGCCTGCTCA CTGTAGATGG GGAGTTAGTG GAGTATGGGC CAATACAGGC GCAGGTGCAC 
CCAGGTCTCG CCACGCTGCT CACTGGGCCT GCAGGTCAAA AGCCACAAGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006541075.1
CDS	126..1979
Translation	MAPPPLLPVAASTPILHGEFGSYPANGPRFALTLTTQALHIQRLRPKPEARPRDGLVSLDEVSGCGTLQSRSPEDTAAYFCIYTYPRGRRGGRRRATRTFRADGATTYEENRAEAQRWATALTCLLRGVPLSGDQEITPELLPRKPRLLILVNPFGGRGLAWQRCMDHVVPMISEAGLSFNLIQTERQNHARELVQGLSLSEWEGIVTVSGDGLLYEVLNGLLDRPDWEDAVRMPIGVLPCGSGNALAGAVNHHGGFEQVVGVDLLLNCSLLLCRGGSHPLDLLSVTLASGSRCFSFLSVAWGFLSDVDIHSERFRALGSARFTLGAVLGLASLHTYRGRLSYLPATTEPALPIPGHSLPRAKSELVLAPAPAPAATHSPLHRSVSDLPLPLPQPALVSPGSPEPLPDLSLNGGGPELTGDWGGAGDAPLSPDPLLPSSPNALKTAQLSPIAEGPPEMPASSGFLPPTHSAPEASTWGPVDHLLPPLGSPLPQDWVTIEGEFVLMLGILPSHLCADLMAAPHARFDDGVVHLCWVRSGISRAALLRILLAMEHGNHFSLGCPHLGYAAARAFRLEPLTPRGLLTVDGELVEYGPIQAQVHPGLATLLTGPAGQKPQA

Target ORF information:

RefSeq Version	XM_006541012.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus sphingosine kinase 2 (Sphk2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006541012.4

ORF Insert Sequence:

ATGGCCCCAC CACCACTACT GCCAGTGGCT GCCAGCACTC CAATCCTGCA CGGCGAGTTT 
GGTTCCTACC CGGCCAACGG CCCACGGTTT GCCCTCACCC TCACAACACA AGCCCTACAC 
ATACAGCGAC TACGCCCAAA GCCAGAAGCC CGGCCCCGAG ATGGTCTAGT CTCTCTGGAT 
GAGGTCTCGG GCTGTGGCAC CCTGCAGAGC CGTAGCCCCG AGGACACTGC AGCCTACTTC 
TGCATCTACA CCTACCCACG TGGCCGTCGA GGGGGCCGGC GCAGAGCTAC GCGGACCTTC 
CGGGCGGATG GGGCCACCAC TTATGAGGAG AATCGTGCAG AGGCCCAGCG CTGGGCCACT 
GCCCTCACGT GTCTCCTCCG AGGAGTGCCT CTGTCAGGGG ACCAGGAAAT CACCCCTGAA 
TTGCTGCCCC GGAAGCCCAG GCTGCTCATA TTGGTCAATC CCTTTGGGGG GCGGGGCCTG 
GCCTGGCAGC GCTGTATGGA CCACGTGGTG CCAATGATCT CTGAAGCTGG GCTGTCCTTC 
AACCTCATAC AGACAGAACG ACAGAACCAT GCCCGTGAGC TGGTGCAGGG GTTAAGCCTG 
AGTGAGTGGG AAGGCATTGT CACTGTGTCT GGAGACGGGC TGCTTTACGA GGTGCTGAAT 
GGGCTCCTTG ATCGGCCAGA CTGGGAGGAT GCCGTGCGGA TGCCCATTGG TGTCCTCCCC 
TGTGGATCGG GCAATGCGCT AGCTGGGGCG GTGAACCATC ATGGCGGGTT TGAGCAGGTT 
GTCGGTGTTG ACCTGTTGCT CAACTGCTCG CTTCTTCTCT GCCGTGGTGG CAGCCATCCT 
CTGGACCTGC TCTCTGTGAC GCTAGCCTCG GGATCCCGCT GTTTTTCCTT CCTGTCAGTG 
GCCTGGGGAT TCTTGTCAGA TGTGGACATT CACAGTGAGC GCTTCAGGGC CCTGGGCAGC 
GCTCGATTCA CACTGGGTGC AGTGCTAGGC CTGGCCTCGT TGCATACCTA CCGTGGACGC 
CTCTCCTACC TCCCCGCTAC CACAGAACCA GCCTTGCCCA TCCCAGGCCA CAGTCTGCCT 
CGAGCCAAGT CAGAACTAGT CTTGGCTCCA GCCCCAGCCC CCGCCGCCAC CCACTCGCCT 
CTACATCGAT CTGTGTCTGA CCTGCCCCTG CCCCTTCCCC AGCCTGCCTT GGTCTCCCCT 
GGCTCCCCTG AGCCCCTGCC TGACCTGTCC CTCAATGGTG GTGGTCCAGA GCTGACTGGA 
GACTGGGGAG GAGCTGGGGA TGCACCTCTG TCCCCAGACC CACTGCTGCC TTCATCCCCC 
AACGCTCTCA AAACAGCTCA GCTTTCACCC ATCGCTGAAG GGCCCCCAGA AATGCCAGCA 
TCTTCGGGGT TCCTGCCTCC CACCCACAGT GCCCCAGAAG CCTCTACCTG GGGCCCAGTG 
GACCACCTCC TCCCTCCCCT GGGCTCTCCA CTGCCCCAAG ACTGGGTGAC AATAGAGGGG 
GAGTTTGTAC TCATGTTGGG CATCTTGCCG AGCCACCTCT GCGCAGACCT GATGGCAGCC 
CCACATGCAC GCTTTGATGA TGGCGTTGTG CACCTGTGTT GGGTGCGGAG CGGCATCTCA 
CGGGCTGCAC TTCTACGCAT TTTGCTGGCC ATGGAGCATG GAAACCACTT CAGCCTGGGC 
TGCCCCCATC TGGGCTATGC TGCAGCACGT GCCTTCCGCC TTGAACCACT CACGCCTCGT 
GGCCTGCTCA CTGTAGATGG GGAGTTAGTG GAGTATGGGC CAATACAGGC GCAGGTGCAC 
CCAGGTCTCG CCACGCTGCT CACTGGGCCT GCAGGTCAAA AGCCACAAGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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Sphk2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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電話

FAX

オンラインフォーム

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