UBE2J1 cDNA ORF clone, Mus musculus(House mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	UBE2J1
Entrez Gene ID	56228
Full Name	ubiquitin-conjugating enzyme E2J 1
Synonyms	0710008M05Rik,1110030I22Rik,NCUBE-1,Ncube,Ncube1,Ubc6p
General protein information	Preferred Names ubiquitin-conjugating enzyme E2J 1 Names ubiquitin-conjugating enzyme E2 J1 E2 ubiquitin-conjugating enzyme J1 non-canonical ubiquitin-conjugating enzyme 1 ubiquitin-conjugating enzyme E2, J1
Gene Type	protein-coding
Organism	Mus musculus(House mouse)
Genome	Chromosome: 4 Map Location: 4\|4 A5

mRNA and Protein(s)

mRNA	Protein	Name
XM_006538107.2	XP_006538170.1	ubiquitin-conjugating enzyme E2 J1 isoform X1
NM_001355494.1	NP_001342423.1	ubiquitin-conjugating enzyme E2 J1 isoform 2
NM_019586.4	NP_062532.2	ubiquitin-conjugating enzyme E2 J1 isoform 1
NM_019586.3	NP_062532.2	ubiquitin-conjugating enzyme E2 J1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	UBE2J1	XP_518636.2
	UBE2J1	XP_001096217.1
Bos taurus (cattle)	UBE2J1	NP_001193115.1
Danio rerio (zebrafish)	ube2j1	NP_999932.1
Xenopus tropicalis (tropical clawed frog)	ube2j1	NP_001011167.1
Homo sapiens (human)	UBE2J1	NP_057105.2
Mus musculus (house mouse)	Ube2j1	NP_062532.2
Gallus gallus (chicken)	UBE2J1	NP_990094.1
Canis lupus familiaris (dog)	UBE2J1	XP_532230.4
Rattus norvegicus (Norway rat)	Ube2j1	NP_001100112.1
Caenorhabditis elegans (roundworm)	ubc-6	NP_001040755.1

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Posttranscriptional Regulation of Glycoprotein Quality Control in the Endoplasmic Reticulum Is Controlled by the E2?Ub-Conjugating Enzyme UBC6e.
Hagiwara M, Ling J, Koenig PA, Ploegh HL
Molecular cell63(5)753-67(2016 Sep)

The E2 ubiquitin-conjugating enzyme UBE2J1 is required for spermiogenesis in mice.
Koenig PA, Nicholls PK, Schmidt FI, Hagiwara M, Maruyama T, Frydman GH, Watson N, Page DC, Ploegh HL
The Journal of biological chemistry289(50)34490-502(2014 Dec)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

Endoplasmic reticulum-associated ubiquitin-conjugating enzyme Ube2j1 is a novel substrate of MK2 (MAPKAP kinase-2) involved in MK2-mediated TNF? production.
Menon MB, Tiedje C, Lafera J, Ronkina N, Konen T, Kotlyarov A, Gaestel M
The Biochemical journal456(2)163-72(2013 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

UBE2J1 is required for spermiogenesis in mice
Title: The E2 ubiquitin-conjugating enzyme UBE2J1 is required for spermiogenesis in mice.

Ube2j1 is phosphorylated in response to lipopolysaccharides in macrophages and contributes to MK2-dependent TNFalpha biosynthesis by an unknown mechanism.
Title: Endoplasmic reticulum-associated ubiquitin-conjugating enzyme Ube2j1 is a novel substrate of MK2 (MAPKAP kinase-2) involved in MK2-mediated TNF? production.

The following UBE2J1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UBE2J1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu11226	NM_019586.4 Latest version!	Mus musculus ubiquitin-conjugating enzyme E2J 1 (Ube2j1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu33060	NM_001355494.1 Latest version!	Mus musculus ubiquitin-conjugating enzyme E2J 1 (Ube2j1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu33060	XM_006538107.2 Latest version!	Mus musculus ubiquitin-conjugating enzyme E2J 1 (Ube2j1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
View Old Accession Versions >>
OMu11226	NM_019586.3	Mus musculus ubiquitin-conjugating enzyme E2J 1 (Ube2j1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

UBE2J1 ( NM_019586.4 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_062532.2 Mus musculus ubiquitin-conjugating enzyme E2J 1 (Ube2j1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu11226
Clone ID Related Accession (Same CDS sequence)	NM_019586.4 , NM_019586.3
Accession Version	NM_019586.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 957bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Mus musculus(house mouse)
Product	ubiquitin-conjugating enzyme E2 J1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL772288.12, BY707310.1, AK149985.1, AL670464.12, AW457749.1, BY416821.1 and CJ279553.1. On Sep 20, 2017 this sequence version replaced NM_019586.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR1660813.126656.1, SRR1660815.21969.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGACCC GCTACAACCT GAAGAGCCCG GCCGTTAAGC GATTGATGAA AGAAGCTGCA 
GAATTGAAAG ATCCAACAGA TCATTACCAC GCTCAGCCTC TGGAGGATAA CCTTTTTGAA 
TGGCACTTCA CAGTTAGAGG GCCCCCAGAT TCCGATTTTG ATGGAGGAGT TTACCATGGA 
CGCATAGTAC TGCCACCAGA GTACCCCATG AAACCACCAA GCATTATCCT GCTAACAGCT 
AATGGACGAT TTGAAGTAGG CAAGAAGATC TGTTTAAGCA TCTCAGGACA TCATCCTGAA 
ACCTGGCAGC CTTCATGGAG CATAAGGACA GCCTTGCTGG CCATCATCGG GTTTATGCCG 
ACTAAAGGAG AGGGGGCCAT AGGTTCTCTA GATTACACAC CCGAGGAGAG AAGAGCGCTT 
GCCAAAAAAT CGCAAGATTT CTGTTGTGAA GGCTGTGGCT CTGCCATGAA GGATGTCCTG 
TTGCCATTAA AATCTGGAAG TGGTTCAAGC CAGGCTGACC AAGAAGCCAA AGAACTGGCC 
AGGCAGATTA GTTTTAAGGC CGAAGTCAAT TCATCTGGAA AGACTATCGC TGAATCAGAC 
TTAAACCAGT GTTTCTCACT AAATGACTCA CAAGACGATT TACCTACAAC ATTCCAGGGT 
GCTACAGCAA GCACATCGTA TGGCGCCCAG AATCCATCAG GAGCACCCTT GCCACAACCT 
ACCCAGCCAG CTCCTAAGAA TACCTCCATG AGCCCTCGGC AGCGCAGAGC CCAGCAACAG 
AGTCAGAGAA GGCCATCCAC TTCCCCCGAT GTGCTCCAAG GCCAGCCACC AAGAGCCCAC 
CACACAGAGC ACGGCGGGTC CGCCATGCTG ATCATCATCC TGACTTTAGC ATTGGCGGCT 
CTTATATTCC GACGAATATA TCTGGCCAAT GAGTACATAT TTGACTTTGA GTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062532.2
CDS	207..1163
Translation	METRYNLKSPAVKRLMKEAAELKDPTDHYHAQPLEDNLFEWHFTVRGPPDSDFDGGVYHGRIVLPPEYPMKPPSIILLTANGRFEVGKKICLSISGHHPETWQPSWSIRTALLAIIGFMPTKGEGAIGSLDYTPEERRALAKKSQDFCCEGCGSAMKDVLLPLKSGSGSSQADQEAKELARQISFKAEVNSSGKTIAESDLNQCFSLNDSQDDLPTTFQGATASTSYGAQNPSGAPLPQPTQPAPKNTSMSPRQRRAQQQSQRRPSTSPDVLQGQPPRAHHTEHGGSAMLIIILTLALAALIFRRIYLANEYIFDFEL

Target ORF information:

RefSeq Version	NM_019586.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin-conjugating enzyme E2J 1 (Ube2j1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019586.4

ORF Insert Sequence:

ATGGAGACCC GCTACAACCT GAAGAGCCCG GCCGTTAAGC GATTGATGAA AGAAGCTGCA 
GAATTGAAAG ATCCAACAGA TCATTACCAC GCTCAGCCTC TGGAGGATAA CCTTTTTGAA 
TGGCACTTCA CAGTTAGAGG GCCCCCAGAT TCCGATTTTG ATGGAGGAGT TTACCATGGA 
CGCATAGTAC TGCCACCAGA GTACCCCATG AAACCACCAA GCATTATCCT GCTAACAGCT 
AATGGACGAT TTGAAGTAGG CAAGAAGATC TGTTTAAGCA TCTCAGGACA TCATCCTGAA 
ACCTGGCAGC CTTCATGGAG CATAAGGACA GCCTTGCTGG CCATCATCGG GTTTATGCCG 
ACTAAAGGAG AGGGGGCCAT AGGTTCTCTA GATTACACAC CCGAGGAGAG AAGAGCGCTT 
GCCAAAAAAT CGCAAGATTT CTGTTGTGAA GGCTGTGGCT CTGCCATGAA GGATGTCCTG 
TTGCCATTAA AATCTGGAAG TGGTTCAAGC CAGGCTGACC AAGAAGCCAA AGAACTGGCC 
AGGCAGATTA GTTTTAAGGC CGAAGTCAAT TCATCTGGAA AGACTATCGC TGAATCAGAC 
TTAAACCAGT GTTTCTCACT AAATGACTCA CAAGACGATT TACCTACAAC ATTCCAGGGT 
GCTACAGCAA GCACATCGTA TGGCGCCCAG AATCCATCAG GAGCACCCTT GCCACAACCT 
ACCCAGCCAG CTCCTAAGAA TACCTCCATG AGCCCTCGGC AGCGCAGAGC CCAGCAACAG 
AGTCAGAGAA GGCCATCCAC TTCCCCCGAT GTGCTCCAAG GCCAGCCACC AAGAGCCCAC 
CACACAGAGC ACGGCGGGTC CGCCATGCTG ATCATCATCC TGACTTTAGC ATTGGCGGCT 
CTTATATTCC GACGAATATA TCTGGCCAAT GAGTACATAT TTGACTTTGA GTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE2J1 ( NM_019586.3 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_062532.2 Mus musculus ubiquitin-conjugating enzyme E2J 1 (Ube2j1), mRNA.

Price & Availability↑

CloneID	OMu11226
Clone ID Related Accession (Same CDS sequence)	NM_019586.4 , NM_019586.3
Accession Version	NM_019586.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 957bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-27
Organism	Mus musculus(House mouse)
Product	ubiquitin-conjugating enzyme E2 J1
Comment	VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY707310.1, AK149985.1 and AL670464.12. On Jan 5, 2006 this sequence version replaced NM_019586.2. ##Evidence-Data-START## Transcript exon combination :: SRR1660813.126656.1, SRR1660815.21969.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGAGACCC GCTACAACCT GAAGAGCCCG GCCGTTAAGC GATTGATGAA AGAAGCTGCA 
GAATTGAAAG ATCCAACAGA TCATTACCAC GCTCAGCCTC TGGAGGATAA CCTTTTTGAA 
TGGCACTTCA CAGTTAGAGG GCCCCCAGAT TCCGATTTTG ATGGAGGAGT TTACCATGGA 
CGCATAGTAC TGCCACCAGA GTACCCCATG AAACCACCAA GCATTATCCT GCTAACAGCT 
AATGGACGAT TTGAAGTAGG CAAGAAGATC TGTTTAAGCA TCTCAGGACA TCATCCTGAA 
ACCTGGCAGC CTTCATGGAG CATAAGGACA GCCTTGCTGG CCATCATCGG GTTTATGCCG 
ACTAAAGGAG AGGGGGCCAT AGGTTCTCTA GATTACACAC CCGAGGAGAG AAGAGCGCTT 
GCCAAAAAAT CGCAAGATTT CTGTTGTGAA GGCTGTGGCT CTGCCATGAA GGATGTCCTG 
TTGCCATTAA AATCTGGAAG TGGTTCAAGC CAGGCTGACC AAGAAGCCAA AGAACTGGCC 
AGGCAGATTA GTTTTAAGGC CGAAGTCAAT TCATCTGGAA AGACTATCGC TGAATCAGAC 
TTAAACCAGT GTTTCTCACT AAATGACTCA CAAGACGATT TACCTACAAC ATTCCAGGGT 
GCTACAGCAA GCACATCGTA TGGCGCCCAG AATCCATCAG GAGCACCCTT GCCACAACCT 
ACCCAGCCAG CTCCTAAGAA TACCTCCATG AGCCCTCGGC AGCGCAGAGC CCAGCAACAG 
AGTCAGAGAA GGCCATCCAC TTCCCCCGAT GTGCTCCAAG GCCAGCCACC AAGAGCCCAC 
CACACAGAGC ACGGCGGGTC CGCCATGCTG ATCATCATCC TGACTTTAGC ATTGGCGGCT 
CTTATATTCC GACGAATATA TCTGGCCAAT GAGTACATAT TTGACTTTGA GTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062532.2
CDS	162..1118
Translation	METRYNLKSPAVKRLMKEAAELKDPTDHYHAQPLEDNLFEWHFTVRGPPDSDFDGGVYHGRIVLPPEYPMKPPSIILLTANGRFEVGKKICLSISGHHPETWQPSWSIRTALLAIIGFMPTKGEGAIGSLDYTPEERRALAKKSQDFCCEGCGSAMKDVLLPLKSGSGSSQADQEAKELARQISFKAEVNSSGKTIAESDLNQCFSLNDSQDDLPTTFQGATASTSYGAQNPSGAPLPQPTQPAPKNTSMSPRQRRAQQQSQRRPSTSPDVLQGQPPRAHHTEHGGSAMLIIILTLALAALIFRRIYLANEYIFDFEL

Target ORF information:

RefSeq Version	NM_019586.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus ubiquitin-conjugating enzyme E2J 1 (Ube2j1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019586.3

ORF Insert Sequence:

ATGGAGACCC GCTACAACCT GAAGAGCCCG GCCGTTAAGC GATTGATGAA AGAAGCTGCA 
GAATTGAAAG ATCCAACAGA TCATTACCAC GCTCAGCCTC TGGAGGATAA CCTTTTTGAA 
TGGCACTTCA CAGTTAGAGG GCCCCCAGAT TCCGATTTTG ATGGAGGAGT TTACCATGGA 
CGCATAGTAC TGCCACCAGA GTACCCCATG AAACCACCAA GCATTATCCT GCTAACAGCT 
AATGGACGAT TTGAAGTAGG CAAGAAGATC TGTTTAAGCA TCTCAGGACA TCATCCTGAA 
ACCTGGCAGC CTTCATGGAG CATAAGGACA GCCTTGCTGG CCATCATCGG GTTTATGCCG 
ACTAAAGGAG AGGGGGCCAT AGGTTCTCTA GATTACACAC CCGAGGAGAG AAGAGCGCTT 
GCCAAAAAAT CGCAAGATTT CTGTTGTGAA GGCTGTGGCT CTGCCATGAA GGATGTCCTG 
TTGCCATTAA AATCTGGAAG TGGTTCAAGC CAGGCTGACC AAGAAGCCAA AGAACTGGCC 
AGGCAGATTA GTTTTAAGGC CGAAGTCAAT TCATCTGGAA AGACTATCGC TGAATCAGAC 
TTAAACCAGT GTTTCTCACT AAATGACTCA CAAGACGATT TACCTACAAC ATTCCAGGGT 
GCTACAGCAA GCACATCGTA TGGCGCCCAG AATCCATCAG GAGCACCCTT GCCACAACCT 
ACCCAGCCAG CTCCTAAGAA TACCTCCATG AGCCCTCGGC AGCGCAGAGC CCAGCAACAG 
AGTCAGAGAA GGCCATCCAC TTCCCCCGAT GTGCTCCAAG GCCAGCCACC AAGAGCCCAC 
CACACAGAGC ACGGCGGGTC CGCCATGCTG ATCATCATCC TGACTTTAGC ATTGGCGGCT 
CTTATATTCC GACGAATATA TCTGGCCAAT GAGTACATAT TTGACTTTGA GTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE2J1 ( NM_001355494.1 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_001342423.1 Mus musculus ubiquitin-conjugating enzyme E2J 1 (Ube2j1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu33060
Clone ID Related Accession (Same CDS sequence)	XM_006538107.2 , NM_001355494.1
Accession Version	NM_001355494.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 837bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-29
Organism	Mus musculus(house mouse)
Product	ubiquitin-conjugating enzyme E2 J1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL772288.12, BY707310.1, AK149985.1, DN175696.1, AL670464.12, AW457749.1, BY416821.1 and CJ279553.1. On Sep 20, 2017 this sequence version replaced XM_006538107.2. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' coding region. The encoded isoform (2) has the same N- and C-termini, but is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1660813.124080.1, SRR1660813.50449.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGACCC GCTACAACCT GAAGAGCCCG GCCGTTAAGC GATTGATGAA AGAAGCTGCA 
GAATTGAAAG ATCCAACAGA TCATTACCAC GCTCAGCCTC TGGAGGATAA CCTTTTTGAA 
TGGCACTTCA CAGTTAGAGG GCCCCCAGAT TCCGATTTTG ATGGAGGAGT TTACCATGGA 
CGCATAGTAC TGCCACCAGA GTACCCCATG AAACCACCAA GCATTATCCT GCTAACAGCT 
AATGGACGAT TTGAAGTAGG CAAGAAGATC TGTTTAAGCA TCTCAGGACA TCATCCTGAA 
ACCTGGCAGC CTTCATGGAG CATAAGGACA GCCTTGCTGG CCATCATCGG GTTTATGCCG 
ACTAAAGGAG AGGGGGCCAT AGGTTCTCTA GATTACACAC CCGAGGAGAG AAGAGCGCTT 
GCCAAAAAAT CGCAAGATTT CTGTTGTGAA GGCTGTGGCT CTGCCATGAA GGATGTCCTG 
TTGCCATTAA AATCTGGAAG TGGTTCAAGC CAGGCTGACC AAGAAGCCAA AGAACTGGCC 
AGGCAGATTA GTTTTAAGTA TGGCGCCCAG AATCCATCAG GAGCACCCTT GCCACAACCT 
ACCCAGCCAG CTCCTAAGAA TACCTCCATG AGCCCTCGGC AGCGCAGAGC CCAGCAACAG 
AGTCAGAGAA GGCCATCCAC TTCCCCCGAT GTGCTCCAAG GCCAGCCACC AAGAGCCCAC 
CACACAGAGC ACGGCGGGTC CGCCATGCTG ATCATCATCC TGACTTTAGC ATTGGCGGCT 
CTTATATTCC GACGAATATA TCTGGCCAAT GAGTACATAT TTGACTTTGA GTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001342423.1
CDS	207..1043
Translation	METRYNLKSPAVKRLMKEAAELKDPTDHYHAQPLEDNLFEWHFTVRGPPDSDFDGGVYHGRIVLPPEYPMKPPSIILLTANGRFEVGKKICLSISGHHPETWQPSWSIRTALLAIIGFMPTKGEGAIGSLDYTPEERRALAKKSQDFCCEGCGSAMKDVLLPLKSGSGSSQADQEAKELARQISFKYGAQNPSGAPLPQPTQPAPKNTSMSPRQRRAQQQSQRRPSTSPDVLQGQPPRAHHTEHGGSAMLIIILTLALAALIFRRIYLANEYIFDFEL

Target ORF information:

RefSeq Version	NM_001355494.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin-conjugating enzyme E2J 1 (Ube2j1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001355494.1

ORF Insert Sequence:

ATGGAGACCC GCTACAACCT GAAGAGCCCG GCCGTTAAGC GATTGATGAA AGAAGCTGCA 
GAATTGAAAG ATCCAACAGA TCATTACCAC GCTCAGCCTC TGGAGGATAA CCTTTTTGAA 
TGGCACTTCA CAGTTAGAGG GCCCCCAGAT TCCGATTTTG ATGGAGGAGT TTACCATGGA 
CGCATAGTAC TGCCACCAGA GTACCCCATG AAACCACCAA GCATTATCCT GCTAACAGCT 
AATGGACGAT TTGAAGTAGG CAAGAAGATC TGTTTAAGCA TCTCAGGACA TCATCCTGAA 
ACCTGGCAGC CTTCATGGAG CATAAGGACA GCCTTGCTGG CCATCATCGG GTTTATGCCG 
ACTAAAGGAG AGGGGGCCAT AGGTTCTCTA GATTACACAC CCGAGGAGAG AAGAGCGCTT 
GCCAAAAAAT CGCAAGATTT CTGTTGTGAA GGCTGTGGCT CTGCCATGAA GGATGTCCTG 
TTGCCATTAA AATCTGGAAG TGGTTCAAGC CAGGCTGACC AAGAAGCCAA AGAACTGGCC 
AGGCAGATTA GTTTTAAGTA TGGCGCCCAG AATCCATCAG GAGCACCCTT GCCACAACCT 
ACCCAGCCAG CTCCTAAGAA TACCTCCATG AGCCCTCGGC AGCGCAGAGC CCAGCAACAG 
AGTCAGAGAA GGCCATCCAC TTCCCCCGAT GTGCTCCAAG GCCAGCCACC AAGAGCCCAC 
CACACAGAGC ACGGCGGGTC CGCCATGCTG ATCATCATCC TGACTTTAGC ATTGGCGGCT 
CTTATATTCC GACGAATATA TCTGGCCAAT GAGTACATAT TTGACTTTGA GTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE2J1 ( XM_006538107.2 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_006538170.1 Mus musculus ubiquitin-conjugating enzyme E2J 1 (Ube2j1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu33060
Clone ID Related Accession (Same CDS sequence)	XM_006538107.2 , NM_001355494.1
Accession Version	XM_006538107.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 837bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	ubiquitin-conjugating enzyme E2 J1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006538107.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACCC GCTACAACCT GAAGAGCCCG GCCGTTAAGC GATTGATGAA AGAAGCTGCA 
GAATTGAAAG ATCCAACAGA TCATTACCAC GCTCAGCCTC TGGAGGATAA CCTTTTTGAA 
TGGCACTTCA CAGTTAGAGG GCCCCCAGAT TCCGATTTTG ATGGAGGAGT TTACCATGGA 
CGCATAGTAC TGCCACCAGA GTACCCCATG AAACCACCAA GCATTATCCT GCTAACAGCT 
AATGGACGAT TTGAAGTAGG CAAGAAGATC TGTTTAAGCA TCTCAGGACA TCATCCTGAA 
ACCTGGCAGC CTTCATGGAG CATAAGGACA GCCTTGCTGG CCATCATCGG GTTTATGCCG 
ACTAAAGGAG AGGGGGCCAT AGGTTCTCTA GATTACACAC CCGAGGAGAG AAGAGCGCTT 
GCCAAAAAAT CGCAAGATTT CTGTTGTGAA GGCTGTGGCT CTGCCATGAA GGATGTCCTG 
TTGCCATTAA AATCTGGAAG TGGTTCAAGC CAGGCTGACC AAGAAGCCAA AGAACTGGCC 
AGGCAGATTA GTTTTAAGTA TGGCGCCCAG AATCCATCAG GAGCACCCTT GCCACAACCT 
ACCCAGCCAG CTCCTAAGAA TACCTCCATG AGCCCTCGGC AGCGCAGAGC CCAGCAACAG 
AGTCAGAGAA GGCCATCCAC TTCCCCCGAT GTGCTCCAAG GCCAGCCACC AAGAGCCCAC 
CACACAGAGC ACGGCGGGTC CGCCATGCTG ATCATCATCC TGACTTTAGC ATTGGCGGCT 
CTTATATTCC GACGAATATA TCTGGCCAAT GAGTACATAT TTGACTTTGA GTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006538170.1
CDS	200..1036
Translation	METRYNLKSPAVKRLMKEAAELKDPTDHYHAQPLEDNLFEWHFTVRGPPDSDFDGGVYHGRIVLPPEYPMKPPSIILLTANGRFEVGKKICLSISGHHPETWQPSWSIRTALLAIIGFMPTKGEGAIGSLDYTPEERRALAKKSQDFCCEGCGSAMKDVLLPLKSGSGSSQADQEAKELARQISFKYGAQNPSGAPLPQPTQPAPKNTSMSPRQRRAQQQSQRRPSTSPDVLQGQPPRAHHTEHGGSAMLIIILTLALAALIFRRIYLANEYIFDFEL

Target ORF information:

RefSeq Version	XM_006538107.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus ubiquitin-conjugating enzyme E2J 1 (Ube2j1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006538107.2

ORF Insert Sequence:

ATGGAGACCC GCTACAACCT GAAGAGCCCG GCCGTTAAGC GATTGATGAA AGAAGCTGCA 
GAATTGAAAG ATCCAACAGA TCATTACCAC GCTCAGCCTC TGGAGGATAA CCTTTTTGAA 
TGGCACTTCA CAGTTAGAGG GCCCCCAGAT TCCGATTTTG ATGGAGGAGT TTACCATGGA 
CGCATAGTAC TGCCACCAGA GTACCCCATG AAACCACCAA GCATTATCCT GCTAACAGCT 
AATGGACGAT TTGAAGTAGG CAAGAAGATC TGTTTAAGCA TCTCAGGACA TCATCCTGAA 
ACCTGGCAGC CTTCATGGAG CATAAGGACA GCCTTGCTGG CCATCATCGG GTTTATGCCG 
ACTAAAGGAG AGGGGGCCAT AGGTTCTCTA GATTACACAC CCGAGGAGAG AAGAGCGCTT 
GCCAAAAAAT CGCAAGATTT CTGTTGTGAA GGCTGTGGCT CTGCCATGAA GGATGTCCTG 
TTGCCATTAA AATCTGGAAG TGGTTCAAGC CAGGCTGACC AAGAAGCCAA AGAACTGGCC 
AGGCAGATTA GTTTTAAGTA TGGCGCCCAG AATCCATCAG GAGCACCCTT GCCACAACCT 
ACCCAGCCAG CTCCTAAGAA TACCTCCATG AGCCCTCGGC AGCGCAGAGC CCAGCAACAG 
AGTCAGAGAA GGCCATCCAC TTCCCCCGAT GTGCTCCAAG GCCAGCCACC AAGAGCCCAC 
CACACAGAGC ACGGCGGGTC CGCCATGCTG ATCATCATCC TGACTTTAGC ATTGGCGGCT 
CTTATATTCC GACGAATATA TCTGGCCAAT GAGTACATAT TTGACTTTGA GTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Posttranscriptional Regulation of Glycoprotein Quality Control in the Endoplasmic Reticulum Is Controlled by the E2?Ub-Conjugating Enzyme UBC6e.
Molecular cell63(5)753-67(2016 Sep)
Hagiwara M,Ling J,Koenig PA,Ploegh HL

The E2 ubiquitin-conjugating enzyme UBE2J1 is required for spermiogenesis in mice.
The Journal of biological chemistry289(50)34490-502(2014 Dec)
Koenig PA,Nicholls PK,Schmidt FI,Hagiwara M,Maruyama T,Frydman GH,Watson N,Page DC,Ploegh HL

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

Endoplasmic reticulum-associated ubiquitin-conjugating enzyme Ube2j1 is a novel substrate of MK2 (MAPKAP kinase-2) involved in MK2-mediated TNF? production.
The Biochemical journal456(2)163-72(2013 Dec)
Menon MB,Tiedje C,Lafera J,Ronkina N,Konen T,Kotlyarov A,Gaestel M

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UBE2J1 cDNA ORF clone, Mus musculus(House mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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