Tdp2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tdp2
Entrez Gene ID	56196
Full Name	tyrosyl-DNA phosphodiesterase 2
Synonyms	D13Ertd656e,Ttrap
General protein information	Preferred Names tyrosyl-DNA phosphodiesterase 2 Names tyrosyl-DNA phosphodiesterase 2 5'-Tyr-DNA phosphodiesterase 5'-tyrosyl-DNA phosphodiesterase TRAF and TNF receptor-associated protein tyr-DNA phosphodiesterase 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 13 Map Location: 13 A3.1\|13 10.7 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_019551.2	NP_062424.1	tyrosyl-DNA phosphodiesterase 2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	TDP2	XP_005640219.1
Mus musculus (house mouse)	Tdp2	NP_062424.1
Gallus gallus (chicken)	TDP2	NP_001263313.1
	TDP2	XP_001105245.2
Danio rerio (zebrafish)	tdp2b	NP_001073171.1
Xenopus tropicalis (tropical clawed frog)	tdp2	NP_001016944.1
Bos taurus (cattle)	TDP2	NP_001098811.1
Rattus norvegicus (Norway rat)	Tdp2	NP_001030119.1
Homo sapiens (human)	TDP2	NP_057698.2
Pan troglodytes (chimpanzee)	TDP2	XP_518271.3
Caenorhabditis elegans (roundworm)	CELE_Y63D3A.4	NP_493461.1

Gene Ontology
Bibliography

Related articles in PubMed

VPg unlinkase/TDP2 in cardiovirus infected cells: Re-localization and proteolytic cleavage.
Maciejewski S, Ullmer W, Semler BL
Virology516139-146(2018 Jan)

Mre11 Is Essential for the Removal of Lethal Topoisomerase 2 Covalent Cleavage Complexes.
Hoa NN, Shimizu T, Zhou ZW, Wang ZQ, Deshpande RA, Paull TT, Akter S, Tsuda M, Furuta R, Tsutsui K, Takeda S, Sasanuma H
Molecular cell64(3)580-592(2016 Nov)

Reversal of DNA damage induced Topoisomerase 2 DNA-protein crosslinks by Tdp2.
Schellenberg MJ, Perera L, Strom CN, Waters CA, Monian B, Appel CD, Vilas CK, Williams JG, Ramsden DA, Williams RS
Nucleic acids research44(8)3829-44(2016 05)

Divergent Requirement for a DNA Repair Enzyme during Enterovirus Infections.
Maciejewski S, Nguyen JH, G?mez-Herreros F, Cort?s-Ledesma F, Caldecott KW, Semler BL
mBio7(1)e01931-15(2015 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The results collectively provide a structural mechanism for Tdp2 engagement of heterogeneous DNA damage that causes Top2 poisoning.
Title: Reversal of DNA damage induced Topoisomerase 2 DNA-protein crosslinks by Tdp2.

data show that TDP2 activity potentiates enterovirus infections and, in the case of coxsackievirus B3, is a required host function following virus infection of mouse cells
Title: Divergent Requirement for a DNA Repair Enzyme during Enterovirus Infections.

our data reveal TDP2-mediated error-free NHEJ as an efficient and accurate mechanism to repair TOP2-induced DSBs
Title: TDP2-dependent non-homologous end-joining protects against topoisomerase II-induced DNA breaks and genome instability in cells and in vivo.

Evidence implicates Tdp2 in the repair of Top1-mediated DNA damage in cultured cells and in vivo, in the absence of Tdp1.
Title: TDP2 promotes repair of topoisomerase I-mediated DNA damage in the absence of TDP1.

the protein associates with TGF-beta receptors and components of the TRAF6-TAK1 signaling module
Title: TTRAP is a novel component of the non-canonical TRAF6-TAK1 TGF-? signaling pathway.

The following Tdp2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tdp2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu11199	NM_019551.2 Latest version!	Mus musculus tyrosyl-DNA phosphodiesterase 2 (Tdp2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tdp2 ( NM_019551.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_062424.1 Mus musculus tyrosyl-DNA phosphodiesterase 2 (Tdp2), mRNA.

Price & Availability↑

CloneID	OMu11199
Clone ID Related Accession (Same CDS sequence)	NM_019551.2
Accession Version	NM_019551.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1113bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Mus musculus(house mouse)
Product	tyrosyl-DNA phosphodiesterase 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY719852.1, BC132511.1 and AL589699.4. On Dec 16, 2009 this sequence version replaced NM_019551.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ251328.1, BC132511.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGTCTG GCAGCAGTTC CGATGCGGCG GAGCCCGCAG GGCCGGCAGG GCGGGCGGCG 
TCGGCGCCCG AAGCAGCACA GGCGGAGGAG GACCGGGTGA AGAGGCGGCG GCTTCAGTGC 
CTGGGCTTTG CGTTGGTGGG GGGATGCGAC CCCACGATGG TCCCCAGCGT CCTGCGGGAG 
AACGACTGGC AGACGCAGAA AGCCCTGAGC GCCTACTTCG AGCTGCCAGA GAACGACCAA 
GGGTGGCCGC GCCAGCCTCC CACGTCCTTC AAGTCCGAGG CCTATGTTGA TCTAACCAAC 
GAGGATGCAA ATGATACAAC CATTTTAGAA GCCAGTCCAT CTGGAACTCC TCTAGAAGAT 
AGCAGCACTA TTTCTTTCAT TACCTGGAAT ATTGATGGAT TAGATGGATG CAATCTGCCC 
GAGAGGGCTC GAGGGGTGTG TTCCTGCCTA GCTTTGTATA GTCCAGATGT GGTATTTCTA 
CAGGAAGTTA TCCCCCCATA CTGTGCCTAC CTAAAGAAGA GAGCAGCCAG TTACACAATT 
ATTACAGGTA ATGAAGAAGG ATATTTCACA GCTATACTAT TGAAGAAAGG AAGAGTGAAA 
TTTAAAAGTC AGGAGATTAT TCCTTTTCCA AATACCAAAA TGATGAGAAA CCTGCTATGC 
GTAAATGTGA GTTTGGGTGG AAATGAATTT TGCCTTATGA CATCCCATTT GGAGAGCACC 
AGAGAACATT CTGCGGAACG AATAAGACAA TTAAAAACTG TTCTTGGAAA AATGCAAGAG 
GCTCCAGATT CAACCACGGT TATATTTGCA GGAGATACAA ATTTAAGAGA TCAAGAAGTT 
ATCAAATGTG GTGGTTTACC TGACAACGTT TTTGATGCCT GGGAATTTTT AGGCAAACCT 
AAACATTGCC AGTATACATG GGATACGAAA GCAAATAACA ACCTCAGGAT CCCTGCTGCT 
TATAAGCATC GTTTTGATCG AATATTTTTC AGAGCAGAAG AGGGGCACCT TATTCCTCAA 
AGTTTAGACC TTGTTGGGTT GGAAAAACTG GACTGTGGTA GATTTCCGAG TGATCACTGG 
GGGCTCTTGT GCACCTTGAA TGTAGTATTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062424.1
CDS	74..1186
Translation	MASGSSSDAAEPAGPAGRAASAPEAAQAEEDRVKRRRLQCLGFALVGGCDPTMVPSVLRENDWQTQKALSAYFELPENDQGWPRQPPTSFKSEAYVDLTNEDANDTTILEASPSGTPLEDSSTISFITWNIDGLDGCNLPERARGVCSCLALYSPDVVFLQEVIPPYCAYLKKRAASYTIITGNEEGYFTAILLKKGRVKFKSQEIIPFPNTKMMRNLLCVNVSLGGNEFCLMTSHLESTREHSAERIRQLKTVLGKMQEAPDSTTVIFAGDTNLRDQEVIKCGGLPDNVFDAWEFLGKPKHCQYTWDTKANNNLRIPAAYKHRFDRIFFRAEEGHLIPQSLDLVGLEKLDCGRFPSDHWGLLCTLNVVL

Target ORF information:

RefSeq Version	NM_019551.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus tyrosyl-DNA phosphodiesterase 2 (Tdp2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019551.2

ORF Insert Sequence:

ATGGCGTCTG GCAGCAGTTC CGATGCGGCG GAGCCCGCAG GGCCGGCAGG GCGGGCGGCG 
TCGGCGCCCG AAGCAGCACA GGCGGAGGAG GACCGGGTGA AGAGGCGGCG GCTTCAGTGC 
CTGGGCTTTG CGTTGGTGGG GGGATGCGAC CCCACGATGG TCCCCAGCGT CCTGCGGGAG 
AACGACTGGC AGACGCAGAA AGCCCTGAGC GCCTACTTCG AGCTGCCAGA GAACGACCAA 
GGGTGGCCGC GCCAGCCTCC CACGTCCTTC AAGTCCGAGG CCTATGTTGA TCTAACCAAC 
GAGGATGCAA ATGATACAAC CATTTTAGAA GCCAGTCCAT CTGGAACTCC TCTAGAAGAT 
AGCAGCACTA TTTCTTTCAT TACCTGGAAT ATTGATGGAT TAGATGGATG CAATCTGCCC 
GAGAGGGCTC GAGGGGTGTG TTCCTGCCTA GCTTTGTATA GTCCAGATGT GGTATTTCTA 
CAGGAAGTTA TCCCCCCATA CTGTGCCTAC CTAAAGAAGA GAGCAGCCAG TTACACAATT 
ATTACAGGTA ATGAAGAAGG ATATTTCACA GCTATACTAT TGAAGAAAGG AAGAGTGAAA 
TTTAAAAGTC AGGAGATTAT TCCTTTTCCA AATACCAAAA TGATGAGAAA CCTGCTATGC 
GTAAATGTGA GTTTGGGTGG AAATGAATTT TGCCTTATGA CATCCCATTT GGAGAGCACC 
AGAGAACATT CTGCGGAACG AATAAGACAA TTAAAAACTG TTCTTGGAAA AATGCAAGAG 
GCTCCAGATT CAACCACGGT TATATTTGCA GGAGATACAA ATTTAAGAGA TCAAGAAGTT 
ATCAAATGTG GTGGTTTACC TGACAACGTT TTTGATGCCT GGGAATTTTT AGGCAAACCT 
AAACATTGCC AGTATACATG GGATACGAAA GCAAATAACA ACCTCAGGAT CCCTGCTGCT 
TATAAGCATC GTTTTGATCG AATATTTTTC AGAGCAGAAG AGGGGCACCT TATTCCTCAA 
AGTTTAGACC TTGTTGGGTT GGAAAAACTG GACTGTGGTA GATTTCCGAG TGATCACTGG 
GGGCTCTTGT GCACCTTGAA TGTAGTATTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

VPg unlinkase/TDP2 in cardiovirus infected cells: Re-localization and proteolytic cleavage.
Virology516139-146(2018 Jan)
Maciejewski S,Ullmer W,Semler BL

Mre11 Is Essential for the Removal of Lethal Topoisomerase 2 Covalent Cleavage Complexes.
Molecular cell64(3)580-592(2016 Nov)
Hoa NN,Shimizu T,Zhou ZW,Wang ZQ,Deshpande RA,Paull TT,Akter S,Tsuda M,Furuta R,Tsutsui K,Takeda S,Sasanuma H

Reversal of DNA damage induced Topoisomerase 2 DNA-protein crosslinks by Tdp2.
Nucleic acids research44(8)3829-44(2016 05)
Schellenberg MJ,Perera L,Strom CN,Waters CA,Monian B,Appel CD,Vilas CK,Williams JG,Ramsden DA,Williams RS

Divergent Requirement for a DNA Repair Enzyme during Enterovirus Infections.
mBio7(1)e01931-15(2015 Dec)
Maciejewski S,Nguyen JH,G?mez-Herreros F,Cort?s-Ledesma F,Caldecott KW,Semler BL

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Tdp2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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