Paf1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Paf1
Entrez Gene ID	54624
Full Name	Paf1, RNA polymerase II complex component
Synonyms	5730511K23Rik,AA675045
General protein information	Preferred Names Paf1, RNA polymerase II complex component Names RNA polymerase II-associated factor 1 homolog Paf1, RNA polymerase II associated factor, homolog
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 B1

mRNA and Protein(s)

mRNA	Protein	Name
NM_019458.3	NP_062331.2	RNA polymerase II-associated factor 1 homolog

Pathways from BioSystems

Homologies

Bos taurus (cattle)	PAF1	NP_001039758.1
Danio rerio (zebrafish)	paf1	NP_001019624.1
Drosophila melanogaster (fruit fly)	atms	NP_649493.1
Homo sapiens (human)	PAF1	NP_061961.2
Mus musculus (house mouse)	Paf1	NP_062331.2
Gallus gallus (chicken)	PAF1	XP_003643545.2
Caenorhabditis elegans (roundworm)	CELE_C55A6.9	NP_505925.1
Xenopus tropicalis (tropical clawed frog)	paf1	NP_989348.1
Canis lupus familiaris (dog)	PAF1	XP_533675.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001302	XP_321844.5
Rattus norvegicus (Norway rat)	Paf1	NP_001020069.1

Gene Ontology
Bibliography

Related articles in PubMed

The interaction of ENL with PAF1 mitigates polycomb silencing and facilitates murine leukemogenesis.
Hetzner K, Garcia-Cuellar MP, B?ttner C, Slany RK
Blood(2017 Dec)

Regulation of transcriptional elongation in pluripotency and cell differentiation by the PHD-finger protein Phf5a.
Strikoudis A, Lazaris C, Trimarchi T, Galvao Neto AL, Yang Y, Ntziachristos P, Rothbart S, Buckley S, Dolgalev I, Stadtfeld M, Strahl BD, Dynlacht BD, Tsirigos A, Aifantis I
Nature cell biology18(11)1127-1138(2016 Nov)

PAF Complex Plays Novel Subunit-Specific Roles in Alternative Cleavage and Polyadenylation.
Yang Y, Li W, Hoque M, Hou L, Shen S, Tian B, Dynlacht BD
PLoS genetics12(1)e1005794(2016 Jan)

CTR9/PAF1c regulates molecular lineage identity, histone H3K36 trimethylation and genomic imprinting during preimplantation development.
Zhang K, Haversat JM, Mager J
Developmental biology383(1)15-27(2013 Nov)

The X-linked intellectual disability protein PHF6 associates with the PAF1 complex and regulates neuronal migration in the mammalian brain.
Zhang C, Mejia LA, Huang J, Valnegri P, Bennett EJ, Anckar J, Jahani-Asl A, Gallardo G, Ikeuchi Y, Yamada T, Rudnicki M, Harper JW, Bonni A
Neuron78(6)986-93(2013 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

link Paf1C with PolII elongation and RNA processing indicates that Paf1C subunits could play roles in controlling transcript length through suppression of PolII accumulation at transcription start site (TSS)-proximal pA sites
Title: PAF Complex Plays Novel Subunit-Specific Roles in Alternative Cleavage and Polyadenylation.

The PAF1 complex is required for mammalian development, likely through regulation of H3K36me3, indicating a functional conservation of the PAF1 complex from yeast to mammals in vivo.
Title: CTR9/PAF1c regulates molecular lineage identity, histone H3K36 trimethylation and genomic imprinting during preimplantation development.

As part of a cell-intrinsic transcriptional pathway, Paf1 regulates neuronal migration in the brain.
Title: The X-linked intellectual disability protein PHF6 associates with the PAF1 complex and regulates neuronal migration in the mammalian brain.

MLL interacts directly with the polymerase associated factor complex (PAFc) via Paf1 and CTR9. PAFc augments MLL and MLL-AF9 mediated transcriptional activation of Hoxa9 and their interaction is essential for leukemogenesis.
Title: The PAF complex synergizes with MLL fusion proteins at HOX loci to promote leukemogenesis.

show that Paf1/PD2 is overexpressed in mouse embryonic stem cells (ESCs) and is involved in the maintenance of mouse ESCs.
Title: RNA polymerase II associated factor 1/PD2 maintains self-renewal by its interaction with Oct3/4 in mouse embryonic stem cells.

The following Paf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Paf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu11009	NM_019458.3 Latest version!	Mus musculus Paf1, RNA polymerase II complex component (Paf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$314.30 $449.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Paf1 ( NM_019458.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_062331.2 Mus musculus Paf1, RNA polymerase II complex component (Paf1), mRNA.

Price & Availability↑

CloneID	OMu11009
Clone ID Related Accession (Same CDS sequence)	NM_019458.3
Accession Version	NM_019458.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1608bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	RNA polymerase II-associated factor 1 homolog
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC149606.6. On Mar 21, 2007 this sequence version replaced NM_019458.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC029843.1, AB041615.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END##

ATGGCGCCCA CCATCCAGAC CCAGGCCCAG AGGGAAGATG GCCACAGGCC CAATTCCCAC 
CGGACCTTGC CTGAGAGGTC TGGAGTAGTC TGCCGAGTCA AGTACTGCAA TAGCCTTCCT 
GACATCCCAT TTGACCCCAA GTTCATCACC TACCCCTTCG ACCAGAACAG GTTTGTTCAG 
TACAAAGCAA CCTCCTTGGA GAAACAGCAC AAACATGACC TCCTGACTGA GCCAGATCTG 
GGGGTCACCA TTGATCTCAT CAACCCTGAC ACCTACCGCA TTGATCCCAA TGTTCTTCTG 
GATCCGGCTG ATGAGAAGCT TCTAGAAGAG GAGATTCAGG CTCCCACCAG CTCTAAGAGA 
TCCCAGCAGC ATGCAAAGGT GGTACCGTGG ATGCGTAAGA CAGAGTATAT CTCCACTGAG 
TTCAACAGAT ATGGCATCTC CAATGAGAAG CCTGAAGTCA AGATTGGGGT TTCTGTGAAA 
CAGCAGTTCA CAGAGGAAGA AATATACAAA GACAGGGACA GCCAAATCAC AGCCATTGAA 
AAGACTTTTG AAGATGCCCA GAAATCGATC TCCCAGCATT ACAGCAAGCC CCGAGTGACA 
CCAGTGGAGG TCATGCCTGT CTTCCCAGAT TTTAAGATGT GGATAAACCC ATGTGCTCAG 
GTCATTTTTG ACTCAGACCC AGCCCCCAAG GACACAAGTG GTGCAGCTGC ATTAGAGATG 
ATGTCTCAGG CCATGATCAG GGGCATGATG GATGAGGAAG GGAACCAGTT TGTGGCTTAT 
TTTCTGCCTG TGGAAGAAAC ACTAAAGAAA CGAAAGCGGG ACCAAGAGGA AGAGATGGAC 
TATGCACCAG ATGATGTGTA TGACTATAAG ATTGCTCGGG AATACAACTG GAATGTGAAG 
AACAAGGCCA GTAAGGGCTA TGAGGAGAAC TATTTCTTCA TCTTCAGAGA GGGTGACGGA 
GTTTACTACA ATGAGCTGGA GACCAGGGTC CGTCTAAGTA AGCGCCGGGC CAAGGCTGGG 
GTTCAGTCAG GTACCAATGC CTTGCTTGTC GTCAAACACC GTGACATGAA TGAGAAGGAA 
TTAGAAGCCC AGGAGGCACG AAAGGCCCAG TTGGAAAACC ACGAACCAGA GGAAGAGGAG 
GAGGAAGAGA TGGAGGCTGA AGAGAAAGAA GCTGGGGGCT CAGATGAGGA ACAGGAGAAG 
GGTAGCAGCA GTGAGAAAGA AGGCAGCGAG GATGAACACT CTGGAAGTGA GAGTGACCGA 
GAAGAGGGTG ACAGAGATGA GGCAAGTGAC AAGAGTGGCA GCGGTGAGGA TGAAAGCAGT 
GAGGATGAAG CACGTGCTGC CCGGGACAAA GAGGAGATTT TTGGTAGTGA TGCTGATTCA 
GAAGATGATG CTGACTCTGA TGATGAGGAC AGAGGGCAGG CCCACAGGGG CAGTGACAAT 
GACTCGGACA GCGGCAGTGA TGGTGGTGGC CAGAGGAGCC GCAGCCAGAG CAGGAGTCGT 
AGCCGGAGTG CCAGCCCCTT CCCCAGTGGC AGTGAGCACT CAGCTCAGGA AGATGGCAGT 
GAAGCAGCAG CCTCTGATTC CAGTGAGGCT GACAGTGACA GTGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062331.2
CDS	213..1820
Translation	MAPTIQTQAQREDGHRPNSHRTLPERSGVVCRVKYCNSLPDIPFDPKFITYPFDQNRFVQYKATSLEKQHKHDLLTEPDLGVTIDLINPDTYRIDPNVLLDPADEKLLEEEIQAPTSSKRSQQHAKVVPWMRKTEYISTEFNRYGISNEKPEVKIGVSVKQQFTEEEIYKDRDSQITAIEKTFEDAQKSISQHYSKPRVTPVEVMPVFPDFKMWINPCAQVIFDSDPAPKDTSGAAALEMMSQAMIRGMMDEEGNQFVAYFLPVEETLKKRKRDQEEEMDYAPDDVYDYKIAREYNWNVKNKASKGYEENYFFIFREGDGVYYNELETRVRLSKRRAKAGVQSGTNALLVVKHRDMNEKELEAQEARKAQLENHEPEEEEEEEMEAEEKEAGGSDEEQEKGSSSEKEGSEDEHSGSESDREEGDRDEASDKSGSGEDESSEDEARAARDKEEIFGSDADSEDDADSDDEDRGQAHRGSDNDSDSGSDGGGQRSRSQSRSRSRSASPFPSGSEHSAQEDGSEAAASDSSEADSDSD

Target ORF information:

RefSeq Version	NM_019458.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Paf1, RNA polymerase II complex component (Paf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019458.3

ORF Insert Sequence:

ATGGCGCCCA CCATCCAGAC CCAGGCCCAG AGGGAAGATG GCCACAGGCC CAATTCCCAC 
CGGACCTTGC CTGAGAGGTC TGGAGTAGTC TGCCGAGTCA AGTACTGCAA TAGCCTTCCT 
GACATCCCAT TTGACCCCAA GTTCATCACC TACCCCTTCG ACCAGAACAG GTTTGTTCAG 
TACAAAGCAA CCTCCTTGGA GAAACAGCAC AAACATGACC TCCTGACTGA GCCAGATCTG 
GGGGTCACCA TTGATCTCAT CAACCCTGAC ACCTACCGCA TTGATCCCAA TGTTCTTCTG 
GATCCGGCTG ATGAGAAGCT TCTAGAAGAG GAGATTCAGG CTCCCACCAG CTCTAAGAGA 
TCCCAGCAGC ATGCAAAGGT GGTACCGTGG ATGCGTAAGA CAGAGTATAT CTCCACTGAG 
TTCAACAGAT ATGGCATCTC CAATGAGAAG CCTGAAGTCA AGATTGGGGT TTCTGTGAAA 
CAGCAGTTCA CAGAGGAAGA AATATACAAA GACAGGGACA GCCAAATCAC AGCCATTGAA 
AAGACTTTTG AAGATGCCCA GAAATCGATC TCCCAGCATT ACAGCAAGCC CCGAGTGACA 
CCAGTGGAGG TCATGCCTGT CTTCCCAGAT TTTAAGATGT GGATAAACCC ATGTGCTCAG 
GTCATTTTTG ACTCAGACCC AGCCCCCAAG GACACAAGTG GTGCAGCTGC ATTAGAGATG 
ATGTCTCAGG CCATGATCAG GGGCATGATG GATGAGGAAG GGAACCAGTT TGTGGCTTAT 
TTTCTGCCTG TGGAAGAAAC ACTAAAGAAA CGAAAGCGGG ACCAAGAGGA AGAGATGGAC 
TATGCACCAG ATGATGTGTA TGACTATAAG ATTGCTCGGG AATACAACTG GAATGTGAAG 
AACAAGGCCA GTAAGGGCTA TGAGGAGAAC TATTTCTTCA TCTTCAGAGA GGGTGACGGA 
GTTTACTACA ATGAGCTGGA GACCAGGGTC CGTCTAAGTA AGCGCCGGGC CAAGGCTGGG 
GTTCAGTCAG GTACCAATGC CTTGCTTGTC GTCAAACACC GTGACATGAA TGAGAAGGAA 
TTAGAAGCCC AGGAGGCACG AAAGGCCCAG TTGGAAAACC ACGAACCAGA GGAAGAGGAG 
GAGGAAGAGA TGGAGGCTGA AGAGAAAGAA GCTGGGGGCT CAGATGAGGA ACAGGAGAAG 
GGTAGCAGCA GTGAGAAAGA AGGCAGCGAG GATGAACACT CTGGAAGTGA GAGTGACCGA 
GAAGAGGGTG ACAGAGATGA GGCAAGTGAC AAGAGTGGCA GCGGTGAGGA TGAAAGCAGT 
GAGGATGAAG CACGTGCTGC CCGGGACAAA GAGGAGATTT TTGGTAGTGA TGCTGATTCA 
GAAGATGATG CTGACTCTGA TGATGAGGAC AGAGGGCAGG CCCACAGGGG CAGTGACAAT 
GACTCGGACA GCGGCAGTGA TGGTGGTGGC CAGAGGAGCC GCAGCCAGAG CAGGAGTCGT 
AGCCGGAGTG CCAGCCCCTT CCCCAGTGGC AGTGAGCACT CAGCTCAGGA AGATGGCAGT 
GAAGCAGCAG CCTCTGATTC CAGTGAGGCT GACAGTGACA GTGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The interaction of ENL with PAF1 mitigates polycomb silencing and facilitates murine leukemogenesis.
Blood(2017 Dec)
Hetzner K,Garcia-Cuellar MP,B?ttner C,Slany RK

Regulation of transcriptional elongation in pluripotency and cell differentiation by the PHD-finger protein Phf5a.
Nature cell biology18(11)1127-1138(2016 Nov)
Strikoudis A,Lazaris C,Trimarchi T,Galvao Neto AL,Yang Y,Ntziachristos P,Rothbart S,Buckley S,Dolgalev I,Stadtfeld M,Strahl BD,Dynlacht BD,Tsirigos A,Aifantis I

PAF Complex Plays Novel Subunit-Specific Roles in Alternative Cleavage and Polyadenylation.
PLoS genetics12(1)e1005794(2016 Jan)
Yang Y,Li W,Hoque M,Hou L,Shen S,Tian B,Dynlacht BD

CTR9/PAF1c regulates molecular lineage identity, histone H3K36 trimethylation and genomic imprinting during preimplantation development.
Developmental biology383(1)15-27(2013 Nov)
Zhang K,Haversat JM,Mager J

The X-linked intellectual disability protein PHF6 associates with the PAF1 complex and regulates neuronal migration in the mammalian brain.
Neuron78(6)986-93(2013 Jun)
Zhang C,Mejia LA,Huang J,Valnegri P,Bennett EJ,Anckar J,Jahani-Asl A,Gallardo G,Ikeuchi Y,Yamada T,Rudnicki M,Harper JW,Bonni A

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Paf1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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