Cd320 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cd320
Entrez Gene ID	54219
Full Name	CD320 antigen
Synonyms	425O18-1,8D6,D17Ertd716e,NG29,TCblR,VLDL
General protein information	Preferred Names CD320 antigen Names CD320 antigen putative VLDL lipoprotein receptor transcobalamin receptor
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17 B1\|17 17.98 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001305169.1	NP_001292098.1	CD320 antigen isoform 2 precursor
NM_019421.3	NP_062294.3	CD320 antigen isoform 1 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	CD320	NP_057663.1
	LOC711130	XP_001099884.2
Canis lupus familiaris (dog)	CD320	XP_003639780.1
Bos taurus (cattle)	CD320	NP_001160030.1
Mus musculus (house mouse)	Cd320	NP_062294.3
Rattus norvegicus (Norway rat)	Cd320	NP_001014223.1

Gene Ontology
Bibliography

Related articles in PubMed

Maternofetal transport of vitamin B
Arora K, Sequeira JM, Quadros EV
FASEB journal : official publication of the Federation of American Societies for Experimental Biology31(7)3098-3106(2017 Jul)

Behavioral alterations are associated with vitamin B12 deficiency in the transcobalamin receptor/CD320 KO mouse.
Arora K, Sequeira JM, Hern?ndez AI, Alarcon JM, Quadros EV
PloS one12(5)e0177156(2017)

A new plasmid-based microRNA inhibitor system that inhibits microRNA families in transgenic mice and cells: a potential new therapeutic reagent.
Cao H, Yu W, Li X, Wang J, Gao S, Holton NE, Eliason S, Sharp T, Amendt BA
Gene therapy23(6)527-42(2016 06)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study evaluated the role of transcobalamin (TC), the transcobalamin receptor (CD320) , and megalin in various tissues during pregnancy.
Title: Maternofetal transport of vitamin B

CD320 role in the hippocampal long-term potentiation and the hippocampal pyramidal neurons.
Title: Behavioral alterations are associated with vitamin B12 deficiency in the transcobalamin receptor/CD320 KO mouse.

Severe cobalamin depletion in the central nervous system (CNS) after birth in TCblR knockouts indicates that TCblR is the only receptor responsible for cobalamin uptake in the CNS
Title: The transcobalamin receptor knockout mouse: a model for vitamin B12 deficiency in the central nervous system.

In vivo, endogenous PCSK9 regulates VLDLR protein levels and triglyceride accumulation in adipose tissue.
Title: Circulating proprotein convertase subtilisin/kexin 9 (PCSK9) regulates VLDLR protein and triglyceride accumulation in visceral adipose tissue.

Data show that thrombospondin-1 (THBS-1) is a novel physiological ligand for ApoER2 and VLDLR, and that lack of THBS-1 results in anatomical abnormalities of the RMS and leads to a reduction of postnatal neuronal precursors entering the OB.
Title: Thrombospondin-1 binds to ApoER2 and VLDL receptor and functions in postnatal neuronal migration.

The following Cd320 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cd320 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu10876	NM_019421.3 Latest version!	Mus musculus CD320 antigen (Cd320), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu69531	NM_001305169.1 Latest version!	Mus musculus CD320 antigen (Cd320), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cd320 ( NM_019421.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_062294.3 Mus musculus CD320 antigen (Cd320), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu10876
Clone ID Related Accession (Same CDS sequence)	NM_019421.3
Accession Version	NM_019421.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 783bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	CD320 antigen isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK078151.1 and AK140204.1. On Feb 25, 2006 this sequence version replaced NM_019421.2. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK140204.1, AK088189.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCGCGGG GCGGAGCTGG GCGGGCAGTG GCCCTGGGCC TGGTGCTGCG GCTCCTCTTT 
GGTCTCAGAA CAGGCCTAGA GGCCGCTCCG GCTCCGGCCC ATACCCGGGT CCAAGTCTCC 
GGCTCTAGAG CTGACTCCTG CCCGACAGAC ACCTTCCAGT GTCTTACCAG TGGCTATTGT 
GTGCCCCTTT CCTGGCGCTG TGATGGGGAC CAGGACTGCT CTGATGGCAG TGACGAGGAA 
GACTGTAGGA TTGAGTCATG TGCTCAGAAT GGGCAGTGCC AACCACAGTC TGCCCTTCCT 
TGCTCCTGTG ACAACATCAG TGGTTGCTCT GATGTCTCTG ACAAGAACCT CAACTGCAGC 
CGCCCACCCT GTCAGGAGAG TGAGCTGCAT TGCATACTGG ATGATGTCTG TATTCCACAC 
ACGTGGCGCT GTGATGGCCA CCCAGACTGT CTTGACTCCA GTGATGAGCT CAGCTGTGAC 
ACTGACACAG AAATTGATAA GATATTCCAG GAGGAGAATG CCACAACTAC AAGGATTTCT 
ACGACCATGG AGAATGAGAC CTCTTTCAGG AATGTAACAT TTACCTCTGC TGGGGACTCA 
TCCCGAAACC CAAGTGCCTA TGGGGTTATT GCAGCTGCTG GGGTGTTGAG TGCCATCCTG 
GTTTCGGCCA CCCTTCTCAT ATTGCTACGC CTCCGAGGTC AGGGTTATCT GCCCCCACCA 
GGGCTGTTGG TGGCTGTGAA GGAGTCGCTG CTGCTGTCAG AAAGGAAAAC CTCTCTGATC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062294.3
CDS	81..863
Translation	MARGGAGRAVALGLVLRLLFGLRTGLEAAPAPAHTRVQVSGSRADSCPTDTFQCLTSGYCVPLSWRCDGDQDCSDGSDEEDCRIESCAQNGQCQPQSALPCSCDNISGCSDVSDKNLNCSRPPCQESELHCILDDVCIPHTWRCDGHPDCLDSSDELSCDTDTEIDKIFQEENATTTRISTTMENETSFRNVTFTSAGDSSRNPSAYGVIAAAGVLSAILVSATLLILLRLRGQGYLPPPGLLVAVKESLLLSERKTSLI

Target ORF information:

RefSeq Version	NM_019421.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus CD320 antigen (Cd320), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019421.3

ORF Insert Sequence:

ATGGCGCGGG GCGGAGCTGG GCGGGCAGTG GCCCTGGGCC TGGTGCTGCG GCTCCTCTTT 
GGTCTCAGAA CAGGCCTAGA GGCCGCTCCG GCTCCGGCCC ATACCCGGGT CCAAGTCTCC 
GGCTCTAGAG CTGACTCCTG CCCGACAGAC ACCTTCCAGT GTCTTACCAG TGGCTATTGT 
GTGCCCCTTT CCTGGCGCTG TGATGGGGAC CAGGACTGCT CTGATGGCAG TGACGAGGAA 
GACTGTAGGA TTGAGTCATG TGCTCAGAAT GGGCAGTGCC AACCACAGTC TGCCCTTCCT 
TGCTCCTGTG ACAACATCAG TGGTTGCTCT GATGTCTCTG ACAAGAACCT CAACTGCAGC 
CGCCCACCCT GTCAGGAGAG TGAGCTGCAT TGCATACTGG ATGATGTCTG TATTCCACAC 
ACGTGGCGCT GTGATGGCCA CCCAGACTGT CTTGACTCCA GTGATGAGCT CAGCTGTGAC 
ACTGACACAG AAATTGATAA GATATTCCAG GAGGAGAATG CCACAACTAC AAGGATTTCT 
ACGACCATGG AGAATGAGAC CTCTTTCAGG AATGTAACAT TTACCTCTGC TGGGGACTCA 
TCCCGAAACC CAAGTGCCTA TGGGGTTATT GCAGCTGCTG GGGTGTTGAG TGCCATCCTG 
GTTTCGGCCA CCCTTCTCAT ATTGCTACGC CTCCGAGGTC AGGGTTATCT GCCCCCACCA 
GGGCTGTTGG TGGCTGTGAA GGAGTCGCTG CTGCTGTCAG AAAGGAAAAC CTCTCTGATC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cd320 ( NM_001305169.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001292098.1 Mus musculus CD320 antigen (Cd320), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu69531
Clone ID Related Accession (Same CDS sequence)	NM_001305169.1
Accession Version	NM_001305169.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 741bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	CD320 antigen isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK131655.1, AK140204.1 and AK078151.1. On or before Feb 26, 2015 this sequence version replaced XM_011251321.1, XM_006525416.2, XM_011241722.1, XM_006524631.2. Transcript Variant: This variant (2) lacks a 5' exon, uses an alternate 5' terminal exon, and initiates translation at an alternate downstream start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK131655.1, KJ663715.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGTGGGCTG GAAGGCTTTT GTTTCTGATT TTGACCTCTC ACCACGCAGG GCTGGTCACC 
CGGTACCTTC TTGCAGCAGG CTCTAGAGCT GACTCCTGCC CGACAGACAC CTTCCAGTGT 
CTTACCAGTG GCTATTGTGT GCCCCTTTCC TGGCGCTGTG ATGGGGACCA GGACTGCTCT 
GATGGCAGTG ACGAGGAAGA CTGTAGGATT GAGTCATGTG CTCAGAATGG GCAGTGCCAA 
CCACAGTCTG CCCTTCCTTG CTCCTGTGAC AACATCAGTG GTTGCTCTGA TGTCTCTGAC 
AAGAACCTCA ACTGCAGCCG CCCACCCTGT CAGGAGAGTG AGCTGCATTG CATACTGGAT 
GATGTCTGTA TTCCACACAC GTGGCGCTGT GATGGCCACC CAGACTGTCT TGACTCCAGT 
GATGAGCTCA GCTGTGACAC TGACACAGAA ATTGATAAGA TATTCCAGGA GGAGAATGCC 
ACAACTACAA GGATTTCTAC GACCATGGAG AATGAGACCT CTTTCAGGAA TGTAACATTT 
ACCTCTGCTG GGGACTCATC CCGAAACCCA AGTGCCTATG GGGTTATTGC AGCTGCTGGG 
GTGTTGAGTG CCATCCTGGT TTCGGCCACC CTTCTCATAT TGCTACGCCT CCGAGGTCAG 
GGTTATCTGC CCCCACCAGG GCTGTTGGTG GCTGTGAAGG AGTCGCTGCT GCTGTCAGAA 
AGGAAAACCT CTCTGATCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001292098.1
CDS	128..868
Translation	MWAGRLLFLILTSHHAGLVTRYLLAAGSRADSCPTDTFQCLTSGYCVPLSWRCDGDQDCSDGSDEEDCRIESCAQNGQCQPQSALPCSCDNISGCSDVSDKNLNCSRPPCQESELHCILDDVCIPHTWRCDGHPDCLDSSDELSCDTDTEIDKIFQEENATTTRISTTMENETSFRNVTFTSAGDSSRNPSAYGVIAAAGVLSAILVSATLLILLRLRGQGYLPPPGLLVAVKESLLLSERKTSLI

Target ORF information:

RefSeq Version	NM_001305169.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus CD320 antigen (Cd320), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001305169.1

ORF Insert Sequence:

ATGTGGGCTG GAAGGCTTTT GTTTCTGATT TTGACCTCTC ACCACGCAGG GCTGGTCACC 
CGGTACCTTC TTGCAGCAGG CTCTAGAGCT GACTCCTGCC CGACAGACAC CTTCCAGTGT 
CTTACCAGTG GCTATTGTGT GCCCCTTTCC TGGCGCTGTG ATGGGGACCA GGACTGCTCT 
GATGGCAGTG ACGAGGAAGA CTGTAGGATT GAGTCATGTG CTCAGAATGG GCAGTGCCAA 
CCACAGTCTG CCCTTCCTTG CTCCTGTGAC AACATCAGTG GTTGCTCTGA TGTCTCTGAC 
AAGAACCTCA ACTGCAGCCG CCCACCCTGT CAGGAGAGTG AGCTGCATTG CATACTGGAT 
GATGTCTGTA TTCCACACAC GTGGCGCTGT GATGGCCACC CAGACTGTCT TGACTCCAGT 
GATGAGCTCA GCTGTGACAC TGACACAGAA ATTGATAAGA TATTCCAGGA GGAGAATGCC 
ACAACTACAA GGATTTCTAC GACCATGGAG AATGAGACCT CTTTCAGGAA TGTAACATTT 
ACCTCTGCTG GGGACTCATC CCGAAACCCA AGTGCCTATG GGGTTATTGC AGCTGCTGGG 
GTGTTGAGTG CCATCCTGGT TTCGGCCACC CTTCTCATAT TGCTACGCCT CCGAGGTCAG 
GGTTATCTGC CCCCACCAGG GCTGTTGGTG GCTGTGAAGG AGTCGCTGCT GCTGTCAGAA 
AGGAAAACCT CTCTGATCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Maternofetal transport of vitamin B
FASEB journal : official publication of the Federation of American Societies for Experimental Biology31(7)3098-3106(2017 Jul)
Arora K,Sequeira JM,Quadros EV

Behavioral alterations are associated with vitamin B12 deficiency in the transcobalamin receptor/CD320 KO mouse.
PloS one12(5)e0177156(2017)
Arora K,Sequeira JM,Hern?ndez AI,Alarcon JM,Quadros EV

A new plasmid-based microRNA inhibitor system that inhibits microRNA families in transgenic mice and cells: a potential new therapeutic reagent.
Gene therapy23(6)527-42(2016 06)
Cao H,Yu W,Li X,Wang J,Gao S,Holton NE,Eliason S,Sharp T,Amendt BA