Adgre4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Adgre4
Entrez Gene ID	52614
Full Name	adhesion G protein-coupled receptor E4
Synonyms	D17Ertd479e,Egf-tm7,Emr4,Fire,Gpr127
General protein information	Preferred Names adhesion G protein-coupled receptor E4 Names adhesion G protein-coupled receptor E4 EGF-like module containing, mucin-like, hormone receptor-like sequence 4 EGF-like module receptor 4 EGF-like module-containing mucin-like hormone receptor-like 4 F4/80-like-receptor seven-span membrane protein FIRE
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17 C-D\|17 28.98 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_139138.3	NP_631877.2	adhesion G protein-coupled receptor E4 precursor

Pathways from BioSystems

Homologies

	LOC100424416	XP_002801089.1
Mus musculus (house mouse)	Emr4	NP_631877.2
Canis lupus familiaris (dog)	EMR4	NP_001033754.2
Rattus norvegicus (Norway rat)	Emr4	NP_001007559.1
	LOC719845	XP_001114593.1
Bos taurus (cattle)	LOC518134	XP_002688945.2

Gene Ontology
Bibliography

Related articles in PubMed

International Union of Basic and Clinical Pharmacology. XCIV. Adhesion G protein-coupled receptors.
Hamann J, Aust G, Ara? D, Engel FB, Formstone C, Fredriksson R, Hall RA, Harty BL, Kirchhoff C, Knapp B, Krishnan A, Liebscher I, Lin HH, Martinelli DC, Monk KR, Peeters MC, Piao X, Pr?mel S, Sch?neberg T, Schwartz TW, Singer K, Stacey M, Ushkaryov YA, Vallon M, Wolfrum U, Wright MW, Xu L, Langenhan T, Schi?th HB
Pharmacological reviews67(2)338-67(2015)

Gene structure and transcript analysis of the human and mouse EGF-TM7 molecule, FIRE.
Caminschi I, Vandenabeele S, Sofi M, McKnight AJ, Ward N, Brodnicki TC, Toy T, Lahoud M, Maraskovsky E, Shortman K, Wright MD
DNA sequence : the journal of DNA sequencing and mapping17(1)8-14(2006 Feb)

The human and mouse repertoire of the adhesion family of G-protein-coupled receptors.
Bjarnad?ttir TK, Fredriksson R, H?glund PJ, Gloriam DE, Lagerstr?m MC, Schi?th HB
Genomics84(1)23-33(2004 Jul)

The EGF-TM7 family: a postgenomic view.
Kwakkenbos MJ, Kop EN, Stacey M, Matmati M, Gordon S, Lin HH, Hamann J
Immunogenetics55(10)655-66(2004 Jan)

EMR4, a novel epidermal growth factor (EGF)-TM7 molecule up-regulated in activated mouse macrophages, binds to a putative cellular ligand on B lymphoma cell line A20.
Stacey M, Chang GW, Sanos SL, Chittenden LR, Stubbs L, Gordon S, Lin HH
The Journal of biological chemistry277(32)29283-93(2002 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Two alternatively spliced human FIRE transcripts were identified, neither are predicted to express the TM7 cassette. Thus, if the human FIRE exists, it is likely to be expressed as a soluble secreted molecule.
Title: Gene structure and transcript analysis of the human and mouse EGF-TM7 molecule, FIRE.

activated in mucosal [and cutaneous]wounds
Title: Different mechanisms of syndecan-1 activation through a fibroblast-growth-factor-inducible response element (FiRE) in mucosal and cutaneous wounds.

the first EGF-TM7 receptor known to mediate the cellular interaction between myeloid cells and B cells
Title: EMR4, a novel epidermal growth factor (EGF)-TM7 molecule up-regulated in activated mouse macrophages, binds to a putative cellular ligand on B lymphoma cell line A20.

The following Adgre4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Adgre4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu10938	NM_139138.3 Latest version!	Mus musculus adhesion G protein-coupled receptor E4 (Adgre4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Adgre4 ( NM_139138.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_631877.2 Mus musculus adhesion G protein-coupled receptor E4 (Adgre4), mRNA.

Price & Availability↑

CloneID	OMu10938
Clone ID Related Accession (Same CDS sequence)	NM_139138.3
Accession Version	NM_139138.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	adhesion G protein-coupled receptor E4 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK036459.1, AF396935.1, AK037483.1 and CT573084.4. On Mar 26, 2009 this sequence version replaced NM_139138.2. Sequence Note: This RefSeq is annotated with the 5'-most in-frame translational start codon. This start codon has a weak Kozak signal and appears to be mouse-specific. Two other possible start codons exist, and their use would reduce the length of the protein N-terminus by 2 aa and 8 aa, respectively. Due to leaky scanning by ribosomes, it is possible that all three start codons are used. PMID:11564768 reports the use of the first start codon, whereas PMID:11564768 reports the use of the third start codon, which has a strong Kozak signal. ##Evidence-Data-START## Transcript exon combination :: AK037483.1, AK036459.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849387, SAMN00849388 [ECO:0000348] ##Evidence-Data-END##

ATGTTGATGG GAGCAACTAG AGATATGGGA AGCAGGTGCC TTCTGCATGC CTCAGTTCCT 
GGAATGCTGC TTATCTGGTC AATATTACAA ATGATGAATA TTTCAGCTTC CTGTCCCCAG 
TGCAATGAAA ATGCCAGCTG CTTCAACAGC ACCCACTGTG TTTGTAAAGA AGGATTCTGG 
ACGGGCTCTG AGAATAGAAG AATTATTGAG CCCCATGAGA AATGTCAAGA TATTAATGAG 
TGTCTACTGA AAGAATTGGT ATGCAAGGAT GTGTCGTACT GCAGAAATAA AATTGGGACT 
TACATATGCA GCTGTGTAGT AAAATATCCT TTGTTCAACT GGGTAGCTGG CATTATTAAT 
ATTGATCACC CTGATTGTTA TGTGAACAAG AGCAAGAATA CAGGATCAAA AACACATACT 
TTGGGAGTAC TGAGTGAATT TAAATCCAAA GAGGAGGTTG CAAAAGGAGC TACCAAGTTA 
CTTCGCAAAG TGGAACATCA CATCTTGAAT GAAAACTCAG ATATACCAAA AAAGGATGAA 
AATCCTTTAT TGGATATAGT GTATGAAACT AAGAGGTGCA AGACGATGAC TCTTCTAGAA 
GCTGGCAACA ACACAATGAA GGTTGACTGC ACTAGTGGTT TCAAAGAGCA CAACAGTGGA 
GGTGAAACTG CAGTGGCTTT CATTGCATAT AAGTCTCTTG GGAATCTTCT AAATGGTTCC 
TTTTTTAGTA ATGAAGAAGG GTTTCAGGAA GTGACACTGA ACTCTCACAT CGTTAGTGGA 
GCCATTCGCT CAGAGGTCAA ACCTGTCCTC TCTGAACCTG TACTCCTGAC TTTACAAAAT 
ATTCAGCCCA TTGACTCAAG AGCAGAACAT CTCTGTGTCC ATTGGGAAGG ATCAGAGGAA 
GGGGGGAGCT GGTCTACCAA AGGATGCTCT CACGTGTACA CCAATAATTC CTACACCATT 
TGCAAGTGTT TCCACCTGTC CAGCTTTGCT GTGCTCATGG CTCTACCCCA TGAGGAGGAT 
GGTGTGCTTT CTGCACTCTC TGTGATCACC TATGTGGGAC TGAGTCTTTC TCTCTTGTGC 
CTATTTCTGG CGGCCATCAC TTTTCTCCTG TGCCGACCCA TTCAGAATAC CAGCACGACA 
CTCCACCTGC AGCTCTCCAT CTGCCTTTTC CTGGCTGACC TCCTCTTCCT CACAGGCATC 
AACAGAACTA AGCCTAAGGT GCTGTGCTCC ATCATAGCGG GGATGTTGCA CTACCTCTAC 
TTGGCTTCCT TCATGTGGAT GTTTCTGGAA GGGCTACATC TTTTTCTCAC TGTGAGCAAT 
CTCAAAGTGG CCAACTACAG CAACTCAGGC AGATTCAAGA AGAGGTTCAT GTATCCTGTA 
GGATATGGGC TTCCTGCTTT TATTGTTGCT GTATCTGCAA TAGCTGGCCA CAAGAATTAT 
GGAACACACA ACCACTGCTG GCTCAGCCTT CATCGAGGAT TCATCTGGAG CTTCTTGGGG 
CCAGCGGCAG CCATTATCTT GATAAACCTG GTGTTCTACT TTCTAATAAT ATGGATTTTG 
AGAAGCAAAC TTTCTTCTCT CAATAAAGAA GTTTCTACAC TTCAAGACAC AAAGGTTATG 
ACATTTAAAG CCATTGTCCA GTTATTTGTG TTGGGATGTT CTTGGGGCAT TGGCTTGTTT 
ATTTTCATTG AAGTTGGGAA GACAGTGAGA CTGATCGTTG CCTATCTGTT CACCATCATC 
AATGTCCTGC AGGGTGTTTT GATATTTATG GTACATTGTC TGCTTAATCG CCAGGTGCGG 
ATGGAATATA AGAAGTGGTT TCATAGACTG CGGAAGGAAG TTGAAAGTGA AAGCACTGAA 
GTGTCTCATT CTACTACTCA CACAAAAATG GGTCTTTCTC TGAACCTGGA AAATTTCTGC 
CCAACAGGAA ACCTCCATGA TCCTTCTGAC TCCATCCTTC CAAGTACTGA AGTAGCAGGT 
GTATATCTAA GCACACCCAG GTCTCACATG GGTGCTGAGG ATGTGAACTC AGGTACTCAC 
GCTTACTGGA GCAGAACTAT TAGTGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_631877.2
CDS	146..2215
Translation	MLMGATRDMGSRCLLHASVPGMLLIWSILQMMNISASCPQCNENASCFNSTHCVCKEGFWTGSENRRIIEPHEKCQDINECLLKELVCKDVSYCRNKIGTYICSCVVKYPLFNWVAGIINIDHPDCYVNKSKNTGSKTHTLGVLSEFKSKEEVAKGATKLLRKVEHHILNENSDIPKKDENPLLDIVYETKRCKTMTLLEAGNNTMKVDCTSGFKEHNSGGETAVAFIAYKSLGNLLNGSFFSNEEGFQEVTLNSHIVSGAIRSEVKPVLSEPVLLTLQNIQPIDSRAEHLCVHWEGSEEGGSWSTKGCSHVYTNNSYTICKCFHLSSFAVLMALPHEEDGVLSALSVITYVGLSLSLLCLFLAAITFLLCRPIQNTSTTLHLQLSICLFLADLLFLTGINRTKPKVLCSIIAGMLHYLYLASFMWMFLEGLHLFLTVSNLKVANYSNSGRFKKRFMYPVGYGLPAFIVAVSAIAGHKNYGTHNHCWLSLHRGFIWSFLGPAAAIILINLVFYFLIIWILRSKLSSLNKEVSTLQDTKVMTFKAIVQLFVLGCSWGIGLFIFIEVGKTVRLIVAYLFTIINVLQGVLIFMVHCLLNRQVRMEYKKWFHRLRKEVESESTEVSHSTTHTKMGLSLNLENFCPTGNLHDPSDSILPSTEVAGVYLSTPRSHMGAEDVNSGTHAYWSRTISD

Target ORF information:

RefSeq Version	NM_139138.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus adhesion G protein-coupled receptor E4 (Adgre4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_139138.3

ORF Insert Sequence:

ATGTTGATGG GAGCAACTAG AGATATGGGA AGCAGGTGCC TTCTGCATGC CTCAGTTCCT 
GGAATGCTGC TTATCTGGTC AATATTACAA ATGATGAATA TTTCAGCTTC CTGTCCCCAG 
TGCAATGAAA ATGCCAGCTG CTTCAACAGC ACCCACTGTG TTTGTAAAGA AGGATTCTGG 
ACGGGCTCTG AGAATAGAAG AATTATTGAG CCCCATGAGA AATGTCAAGA TATTAATGAG 
TGTCTACTGA AAGAATTGGT ATGCAAGGAT GTGTCGTACT GCAGAAATAA AATTGGGACT 
TACATATGCA GCTGTGTAGT AAAATATCCT TTGTTCAACT GGGTAGCTGG CATTATTAAT 
ATTGATCACC CTGATTGTTA TGTGAACAAG AGCAAGAATA CAGGATCAAA AACACATACT 
TTGGGAGTAC TGAGTGAATT TAAATCCAAA GAGGAGGTTG CAAAAGGAGC TACCAAGTTA 
CTTCGCAAAG TGGAACATCA CATCTTGAAT GAAAACTCAG ATATACCAAA AAAGGATGAA 
AATCCTTTAT TGGATATAGT GTATGAAACT AAGAGGTGCA AGACGATGAC TCTTCTAGAA 
GCTGGCAACA ACACAATGAA GGTTGACTGC ACTAGTGGTT TCAAAGAGCA CAACAGTGGA 
GGTGAAACTG CAGTGGCTTT CATTGCATAT AAGTCTCTTG GGAATCTTCT AAATGGTTCC 
TTTTTTAGTA ATGAAGAAGG GTTTCAGGAA GTGACACTGA ACTCTCACAT CGTTAGTGGA 
GCCATTCGCT CAGAGGTCAA ACCTGTCCTC TCTGAACCTG TACTCCTGAC TTTACAAAAT 
ATTCAGCCCA TTGACTCAAG AGCAGAACAT CTCTGTGTCC ATTGGGAAGG ATCAGAGGAA 
GGGGGGAGCT GGTCTACCAA AGGATGCTCT CACGTGTACA CCAATAATTC CTACACCATT 
TGCAAGTGTT TCCACCTGTC CAGCTTTGCT GTGCTCATGG CTCTACCCCA TGAGGAGGAT 
GGTGTGCTTT CTGCACTCTC TGTGATCACC TATGTGGGAC TGAGTCTTTC TCTCTTGTGC 
CTATTTCTGG CGGCCATCAC TTTTCTCCTG TGCCGACCCA TTCAGAATAC CAGCACGACA 
CTCCACCTGC AGCTCTCCAT CTGCCTTTTC CTGGCTGACC TCCTCTTCCT CACAGGCATC 
AACAGAACTA AGCCTAAGGT GCTGTGCTCC ATCATAGCGG GGATGTTGCA CTACCTCTAC 
TTGGCTTCCT TCATGTGGAT GTTTCTGGAA GGGCTACATC TTTTTCTCAC TGTGAGCAAT 
CTCAAAGTGG CCAACTACAG CAACTCAGGC AGATTCAAGA AGAGGTTCAT GTATCCTGTA 
GGATATGGGC TTCCTGCTTT TATTGTTGCT GTATCTGCAA TAGCTGGCCA CAAGAATTAT 
GGAACACACA ACCACTGCTG GCTCAGCCTT CATCGAGGAT TCATCTGGAG CTTCTTGGGG 
CCAGCGGCAG CCATTATCTT GATAAACCTG GTGTTCTACT TTCTAATAAT ATGGATTTTG 
AGAAGCAAAC TTTCTTCTCT CAATAAAGAA GTTTCTACAC TTCAAGACAC AAAGGTTATG 
ACATTTAAAG CCATTGTCCA GTTATTTGTG TTGGGATGTT CTTGGGGCAT TGGCTTGTTT 
ATTTTCATTG AAGTTGGGAA GACAGTGAGA CTGATCGTTG CCTATCTGTT CACCATCATC 
AATGTCCTGC AGGGTGTTTT GATATTTATG GTACATTGTC TGCTTAATCG CCAGGTGCGG 
ATGGAATATA AGAAGTGGTT TCATAGACTG CGGAAGGAAG TTGAAAGTGA AAGCACTGAA 
GTGTCTCATT CTACTACTCA CACAAAAATG GGTCTTTCTC TGAACCTGGA AAATTTCTGC 
CCAACAGGAA ACCTCCATGA TCCTTCTGAC TCCATCCTTC CAAGTACTGA AGTAGCAGGT 
GTATATCTAA GCACACCCAG GTCTCACATG GGTGCTGAGG ATGTGAACTC AGGTACTCAC 
GCTTACTGGA GCAGAACTAT TAGTGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

International Union of Basic and Clinical Pharmacology. XCIV. Adhesion G protein-coupled receptors.
Pharmacological reviews67(2)338-67(2015)
Hamann J,Aust G,Ara? D,Engel FB,Formstone C,Fredriksson R,Hall RA,Harty BL,Kirchhoff C,Knapp B,Krishnan A,Liebscher I,Lin HH,Martinelli DC,Monk KR,Peeters MC,Piao X,Pr?mel S,Sch?neberg T,Schwartz TW,Singer K,Stacey M,Ushkaryov YA,Vallon M,Wolfrum U,Wright MW,Xu L,Langenhan T,Schi?th HB

Gene structure and transcript analysis of the human and mouse EGF-TM7 molecule, FIRE.
DNA sequence : the journal of DNA sequencing and mapping17(1)8-14(2006 Feb)
Caminschi I,Vandenabeele S,Sofi M,McKnight AJ,Ward N,Brodnicki TC,Toy T,Lahoud M,Maraskovsky E,Shortman K,Wright MD

The human and mouse repertoire of the adhesion family of G-protein-coupled receptors.
Genomics84(1)23-33(2004 Jul)
Bjarnad?ttir TK,Fredriksson R,H?glund PJ,Gloriam DE,Lagerstr?m MC,Schi?th HB

The EGF-TM7 family: a postgenomic view.
Immunogenetics55(10)655-66(2004 Jan)
Kwakkenbos MJ,Kop EN,Stacey M,Matmati M,Gordon S,Lin HH,Hamann J

EMR4, a novel epidermal growth factor (EGF)-TM7 molecule up-regulated in activated mouse macrophages, binds to a putative cellular ligand on B lymphoma cell line A20.
The Journal of biological chemistry277(32)29283-93(2002 Aug)
Stacey M,Chang GW,Sanos SL,Chittenden LR,Stubbs L,Gordon S,Lin HH