Spast cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Spast
Entrez Gene ID	50850
Full Name	spastin
Synonyms	Spg4,mKIAA1083
General protein information	Preferred Names spastin Names spastin spastic paraplegia 4 homolog
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17\|17 E2

mRNA and Protein(s)

mRNA	Protein	Name
XM_006524610.3	XP_006524673.1	spastin isoform X1
XM_006524610.4	XP_006524673.1	spastin isoform X1
XM_011246530.2	XP_011244832.1	spastin isoform X3
NM_001357738.1	NP_001344667.1	spastin isoform 3
NM_001162870.1	NP_001156342.1	spastin isoform 1
XM_006524611.3	XP_006524674.1	spastin isoform X2
XM_011246530.3	XP_011244832.1	spastin isoform X2
NM_016962.2	NP_058658.2	spastin isoform 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	SPAST	NP_055761.2
Oryza sativa (rice)	Os06g0130000	NP_001056686.1
Xenopus tropicalis (tropical clawed frog)	spast	NP_001016453.1
	SPAST	XP_002799248.1
Canis lupus familiaris (dog)	SPAST	XP_850973.1
Drosophila melanogaster (fruit fly)	spas	NP_651206.3
Pan troglodytes (chimpanzee)	SPAST	XP_001164367.1
Mus musculus (house mouse)	Spast	NP_001156342.1
Rattus norvegicus (Norway rat)	Spast	NP_001102172.2
Danio rerio (zebrafish)	spast	NP_998080.1
Arabidopsis thaliana (thale cress)	AT2G45500	NP_182074.4
Bos taurus (cattle)	SPAST	NP_001075060.1
Gallus gallus (chicken)	SPAST	NP_001026232.1
Anopheles gambiae (African malaria mosquito)	SPAST_ANOGA	XP_312634.5
Caenorhabditis elegans (roundworm)	spas-1	NP_741586.1

Gene Ontology
Bibliography

Related articles in PubMed

Spastin regulates VAMP7-containing vesicles trafficking in cortical neurons.
Plaud C, Joshi V, Marinello M, Pastr? D, Galli T, Curmi PA, Burgo A
Biochimica et biophysica acta1863(6)1666-1677(2017 Jun)

Branch-Specific Microtubule Destabilization Mediates Axon Branch Loss during Neuromuscular Synapse Elimination.
Brill MS, Kleele T, Ruschkies L, Wang M, Marahori NA, Reuter MS, Hausrat TJ, Weigand E, Fisher M, Ahles A, Engelhardt S, Bishop DL, Kneussel M, Misgeld T
Neuron92(4)845-856(2016 Nov)

Amyloid-? oligomers induce synaptic damage via Tau-dependent microtubule severing by TTLL6 and spastin.
Zempel H, Luedtke J, Kumar Y, Biernat J, Dawson H, Mandelkow E, Mandelkow EM
The EMBO journal32(22)2920-37(2013 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Axon branch loss at the developing mouse neuromuscular junction is mediated by branch-specific microtubule severing, mediated by the microtubule-severing enzyme spastin.
Title: Branch-Specific Microtubule Destabilization Mediates Axon Branch Loss during Neuromuscular Synapse Elimination.

Spastin is recruited by microtubules polyglutamylation, induced by Tau missorting triggered translocalization of TTLL6 (Tubulin-Tyrosine-Ligase-Like-6) into dendrites
Title: Amyloid-? oligomers induce synaptic damage via Tau-dependent microtubule severing by TTLL6 and spastin.

early and marked impairment of microtubule dynamics all along the axons of spastin-deficient cortical neurons, which is likely to be responsible for the occurrence of axonal swellings and cargo stalling
Title: Microtubule-targeting drugs rescue axonal swellings in cortical neurons from spastin knockout mice.

we aimed to identify the critical promoter regions of SPG4 gene and effects of Elk1 on SPG4 gene expression
Title: SPG4 gene promoter regulation via Elk1 transcription factor.

These results strongly support a direct role for defective axonal transport in the pathogenesis of HSP because of spastin mutation.
Title: Direct evidence for axonal transport defects in a novel mouse model of mutant spastin-induced hereditary spastic paraplegia (HSP) and human HSP patients.

The following Spast gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Spast cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu69395	XM_011246530.3 Latest version!	Mus musculus spastin (Spast), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu53827	XM_006524610.4 Latest version!	Mus musculus spastin (Spast), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu08507	NM_001162870.1 Latest version!	Mus musculus spastin (Spast), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
OMu10452	NM_016962.2 Latest version!	Mus musculus spastin (Spast), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
OMu53828	NM_001357738.1 Latest version!	Mus musculus spastin (Spast), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
OMu53828	XM_006524611.3 Latest version!	Mus musculus spastin (Spast), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
View Old Accession Versions >>
OMu53827	XM_006524610.3	Mus musculus spastin (Spast), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
OMu69395	XM_011246530.2	Mus musculus spastin (Spast), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Spast ( XM_011246530.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244832.1 Mus musculus spastin (Spast), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu69395
Clone ID Related Accession (Same CDS sequence)	XM_011246530.2 , XM_011246530.3
Accession Version	XM_011246530.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1032bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	spastin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011246530.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTTCTG GAGCAAGACC GGGACCTGGT CCTGCAGCTA CCACACATAA GGGTACTCCA 
AAACCAAATA GAACCAACAA ACCTTCTACT CCCACAACTG CAGTTCGGAA AAAGAAAGAC 
TTGAAAAATT TTAGGAATGT GGACAGCAAT CTTGCTAACC TTATAATGAA TGAAATTGTT 
GACAATGGGA CAGCTGTTAA GTTTGATGAC ATAGCCGGGC AGGAGCTGGC AAAGCAAGCG 
CTGCAGGAGA TTGTCATCCT TCCTTCTCTG CGGCCTGAGT TGTTCACAGG GCTCAGAGCT 
CCTGCTAGAG GCTTGTTACT CTTCGGTCCG CCAGGAAACG GAAAAACAAT GCTGGCTAAA 
GCAGTAGCTG CAGAGTCTAA TGCGACCTTT TTCAACATAA GTGCTGCCAG TTTAACTTCA 
AAATATGTGG GAGAAGGAGA GAAATTGGTG AGAGCTCTCT TTGCTGTGGC TCGAGAACTT 
CAACCATCTA TAATTTTTAT AGATGAAGTT GACAGTCTTT TGTGTGAGAG ACGGGAAGGG 
GAGCACGACG CTAGCAGACG GCTAAAGACG GAATTTTTAA TAGAATTTGA CGGGGTGCAA 
TCTGCTGGAG ATGACAGAGT ACTTGTAATG GGTGCAACTA ACAGGCCCCA AGAGCTTGAT 
GAAGCTGTTC TCAGGCGTTT CATTAAACGG GTATATGTGT CCTTACCAAA TGAGGAGACA 
AGACTCCTTC TGCTTAAAAA CCTGTTGTGT AAACAAGGAA GTCCACTGAC CCAAAAAGAA 
CTCGCACAGC TTGCTAGAAT GACCGATGGA TACTCTGGAA GTGATCTGAC CGCTTTGGCC 
AAGGATGCAG CCCTGGGTCC TATCCGAGAA CTGAAGCCAG AGCAGGTGAA GAATATGTCT 
GCCAGTGAGA TGAGAAATAT TCGATTATCT GACTTCACAG AATCCTTAAA AAAGATAAAA 
CGCAGTGTGA GTCCTCAGAC CTTAGAAGCA TACATACGCT GGAACAAGGA TTTTGGAGAC 
ACCACTGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244832.1
CDS	743..1774
Translation	MVSGARPGPGPAATTHKGTPKPNRTNKPSTPTTAVRKKKDLKNFRNVDSNLANLIMNEIVDNGTAVKFDDIAGQELAKQALQEIVILPSLRPELFTGLRAPARGLLLFGPPGNGKTMLAKAVAAESNATFFNISAASLTSKYVGEGEKLVRALFAVARELQPSIIFIDEVDSLLCERREGEHDASRRLKTEFLIEFDGVQSAGDDRVLVMGATNRPQELDEAVLRRFIKRVYVSLPNEETRLLLLKNLLCKQGSPLTQKELAQLARMTDGYSGSDLTALAKDAALGPIRELKPEQVKNMSASEMRNIRLSDFTESLKKIKRSVSPQTLEAYIRWNKDFGDTTV

Target ORF information:

RefSeq Version	XM_011246530.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus spastin (Spast), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011246530.2

ORF Insert Sequence:

ATGGTTTCTG GAGCAAGACC GGGACCTGGT CCTGCAGCTA CCACACATAA GGGTACTCCA 
AAACCAAATA GAACCAACAA ACCTTCTACT CCCACAACTG CAGTTCGGAA AAAGAAAGAC 
TTGAAAAATT TTAGGAATGT GGACAGCAAT CTTGCTAACC TTATAATGAA TGAAATTGTT 
GACAATGGGA CAGCTGTTAA GTTTGATGAC ATAGCCGGGC AGGAGCTGGC AAAGCAAGCG 
CTGCAGGAGA TTGTCATCCT TCCTTCTCTG CGGCCTGAGT TGTTCACAGG GCTCAGAGCT 
CCTGCTAGAG GCTTGTTACT CTTCGGTCCG CCAGGAAACG GAAAAACAAT GCTGGCTAAA 
GCAGTAGCTG CAGAGTCTAA TGCGACCTTT TTCAACATAA GTGCTGCCAG TTTAACTTCA 
AAATATGTGG GAGAAGGAGA GAAATTGGTG AGAGCTCTCT TTGCTGTGGC TCGAGAACTT 
CAACCATCTA TAATTTTTAT AGATGAAGTT GACAGTCTTT TGTGTGAGAG ACGGGAAGGG 
GAGCACGACG CTAGCAGACG GCTAAAGACG GAATTTTTAA TAGAATTTGA CGGGGTGCAA 
TCTGCTGGAG ATGACAGAGT ACTTGTAATG GGTGCAACTA ACAGGCCCCA AGAGCTTGAT 
GAAGCTGTTC TCAGGCGTTT CATTAAACGG GTATATGTGT CCTTACCAAA TGAGGAGACA 
AGACTCCTTC TGCTTAAAAA CCTGTTGTGT AAACAAGGAA GTCCACTGAC CCAAAAAGAA 
CTCGCACAGC TTGCTAGAAT GACCGATGGA TACTCTGGAA GTGATCTGAC CGCTTTGGCC 
AAGGATGCAG CCCTGGGTCC TATCCGAGAA CTGAAGCCAG AGCAGGTGAA GAATATGTCT 
GCCAGTGAGA TGAGAAATAT TCGATTATCT GACTTCACAG AATCCTTAAA AAAGATAAAA 
CGCAGTGTGA GTCCTCAGAC CTTAGAAGCA TACATACGCT GGAACAAGGA TTTTGGAGAC 
ACCACTGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spast ( XM_011246530.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244832.1 Mus musculus spastin (Spast), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu69395
Clone ID Related Accession (Same CDS sequence)	XM_011246530.2 , XM_011246530.3
Accession Version	XM_011246530.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1032bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	spastin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011246530.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTTCTG GAGCAAGACC GGGACCTGGT CCTGCAGCTA CCACACATAA GGGTACTCCA 
AAACCAAATA GAACCAACAA ACCTTCTACT CCCACAACTG CAGTTCGGAA AAAGAAAGAC 
TTGAAAAATT TTAGGAATGT GGACAGCAAT CTTGCTAACC TTATAATGAA TGAAATTGTT 
GACAATGGGA CAGCTGTTAA GTTTGATGAC ATAGCCGGGC AGGAGCTGGC AAAGCAAGCG 
CTGCAGGAGA TTGTCATCCT TCCTTCTCTG CGGCCTGAGT TGTTCACAGG GCTCAGAGCT 
CCTGCTAGAG GCTTGTTACT CTTCGGTCCG CCAGGAAACG GAAAAACAAT GCTGGCTAAA 
GCAGTAGCTG CAGAGTCTAA TGCGACCTTT TTCAACATAA GTGCTGCCAG TTTAACTTCA 
AAATATGTGG GAGAAGGAGA GAAATTGGTG AGAGCTCTCT TTGCTGTGGC TCGAGAACTT 
CAACCATCTA TAATTTTTAT AGATGAAGTT GACAGTCTTT TGTGTGAGAG ACGGGAAGGG 
GAGCACGACG CTAGCAGACG GCTAAAGACG GAATTTTTAA TAGAATTTGA CGGGGTGCAA 
TCTGCTGGAG ATGACAGAGT ACTTGTAATG GGTGCAACTA ACAGGCCCCA AGAGCTTGAT 
GAAGCTGTTC TCAGGCGTTT CATTAAACGG GTATATGTGT CCTTACCAAA TGAGGAGACA 
AGACTCCTTC TGCTTAAAAA CCTGTTGTGT AAACAAGGAA GTCCACTGAC CCAAAAAGAA 
CTCGCACAGC TTGCTAGAAT GACCGATGGA TACTCTGGAA GTGATCTGAC CGCTTTGGCC 
AAGGATGCAG CCCTGGGTCC TATCCGAGAA CTGAAGCCAG AGCAGGTGAA GAATATGTCT 
GCCAGTGAGA TGAGAAATAT TCGATTATCT GACTTCACAG AATCCTTAAA AAAGATAAAA 
CGCAGTGTGA GTCCTCAGAC CTTAGAAGCA TACATACGCT GGAACAAGGA TTTTGGAGAC 
ACCACTGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244832.1
CDS	1933..2964
Translation	MVSGARPGPGPAATTHKGTPKPNRTNKPSTPTTAVRKKKDLKNFRNVDSNLANLIMNEIVDNGTAVKFDDIAGQELAKQALQEIVILPSLRPELFTGLRAPARGLLLFGPPGNGKTMLAKAVAAESNATFFNISAASLTSKYVGEGEKLVRALFAVARELQPSIIFIDEVDSLLCERREGEHDASRRLKTEFLIEFDGVQSAGDDRVLVMGATNRPQELDEAVLRRFIKRVYVSLPNEETRLLLLKNLLCKQGSPLTQKELAQLARMTDGYSGSDLTALAKDAALGPIRELKPEQVKNMSASEMRNIRLSDFTESLKKIKRSVSPQTLEAYIRWNKDFGDTTV

Target ORF information:

RefSeq Version	XM_011246530.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus spastin (Spast), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011246530.3

ORF Insert Sequence:

ATGGTTTCTG GAGCAAGACC GGGACCTGGT CCTGCAGCTA CCACACATAA GGGTACTCCA 
AAACCAAATA GAACCAACAA ACCTTCTACT CCCACAACTG CAGTTCGGAA AAAGAAAGAC 
TTGAAAAATT TTAGGAATGT GGACAGCAAT CTTGCTAACC TTATAATGAA TGAAATTGTT 
GACAATGGGA CAGCTGTTAA GTTTGATGAC ATAGCCGGGC AGGAGCTGGC AAAGCAAGCG 
CTGCAGGAGA TTGTCATCCT TCCTTCTCTG CGGCCTGAGT TGTTCACAGG GCTCAGAGCT 
CCTGCTAGAG GCTTGTTACT CTTCGGTCCG CCAGGAAACG GAAAAACAAT GCTGGCTAAA 
GCAGTAGCTG CAGAGTCTAA TGCGACCTTT TTCAACATAA GTGCTGCCAG TTTAACTTCA 
AAATATGTGG GAGAAGGAGA GAAATTGGTG AGAGCTCTCT TTGCTGTGGC TCGAGAACTT 
CAACCATCTA TAATTTTTAT AGATGAAGTT GACAGTCTTT TGTGTGAGAG ACGGGAAGGG 
GAGCACGACG CTAGCAGACG GCTAAAGACG GAATTTTTAA TAGAATTTGA CGGGGTGCAA 
TCTGCTGGAG ATGACAGAGT ACTTGTAATG GGTGCAACTA ACAGGCCCCA AGAGCTTGAT 
GAAGCTGTTC TCAGGCGTTT CATTAAACGG GTATATGTGT CCTTACCAAA TGAGGAGACA 
AGACTCCTTC TGCTTAAAAA CCTGTTGTGT AAACAAGGAA GTCCACTGAC CCAAAAAGAA 
CTCGCACAGC TTGCTAGAAT GACCGATGGA TACTCTGGAA GTGATCTGAC CGCTTTGGCC 
AAGGATGCAG CCCTGGGTCC TATCCGAGAA CTGAAGCCAG AGCAGGTGAA GAATATGTCT 
GCCAGTGAGA TGAGAAATAT TCGATTATCT GACTTCACAG AATCCTTAAA AAAGATAAAA 
CGCAGTGTGA GTCCTCAGAC CTTAGAAGCA TACATACGCT GGAACAAGGA TTTTGGAGAC 
ACCACTGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spast ( XM_006524610.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524673.1 Mus musculus spastin (Spast), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu53827
Clone ID Related Accession (Same CDS sequence)	XM_006524610.3 , XM_006524610.4
Accession Version	XM_006524610.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1749bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	spastin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006524610.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTCTC CGGCCGGACG ACGGAAGAAG AAAGGCTCGG GCGGCGCGAG CCCGGCGCCC 
GCCAGGCCTC CGCCCCCCGC CGCGGTCCCC GCCCCTGCCG CCGGCCCGGC CCCTGCGGCC 
GGCTCGCCGC CTAAGCGGAA CCCGTCTTCT TTCTCGTCCC CGCTGGTCGT CGGCTTCGCC 
CTGCTGCGCC TGCTGGCCTG CCACCTGGGG CTCCTCTTCG CGTGGCTCTG CCAGCGCTTC 
TCCCGCGCCC TCATGGCCGC CAAGAGGAGC TCCGGGACCG CGCCGGCGCC CGCCTCGCCC 
TCGCCCCCAG AGCCCGGACC GGGTGGCGAG GCCGAGAGCG TCCGCGTCTT CCACAAGCAG 
GCCTTCGAGT ACATCTCCAT TGCCCTGCGC ATCGACGAGG AAGAGAAAGC AGGACAGAAG 
GAACAAGCTG TGGAATGGTA TAAGAAAGGT ATCGAAGAAC TGGAAAAAGG AATCGCTGTT 
ATAGTTACGG GCCAAGGTGA ACAGTATGAA AGAGCTAGAC GTCTTCAAGC CAAAATGATG 
ACTAATTTAG TTATGGCCAA GGACCGTTTA CAACTTCTAG AAAGTGGAGC AGTTCCGAAG 
AGGAAAGACC CCTTAACACA TGCTAGTAAT TCATTGCCTC GATCAAAAAC TGTCCTGAAA 
AGTGGCTCCG CAGGGCTCTC CGGTCACCAC AGGGCGCCTA GTTGCAGTGG TTTGTCCATG 
GTTTCTGGAG CAAGACCGGG ACCTGGTCCT GCAGCTACCA CACATAAGGG TACTCCAAAA 
CCAAATAGAA CCAACAAACC TTCTACTCCC ACAACTGCAG TTCGGAAAAA GAAAGACTTG 
AAAAATTTTA GGAATGTGGA CAGCAATCTT GCTAACCTTA TAATGAATGA AATTGTTGAC 
AATGGGACAG CTGTTAAGTT TGATGACATA GCCGGGCAGG AGCTGGCAAA GCAAGCGCTG 
CAGGAGATTG TCATCCTTCC TTCTCTGCGG CCTGAGTTGT TCACAGGGCT CAGAGCTCCT 
GCTAGAGGCT TGTTACTCTT CGGTCCGCCA GGAAACGGAA AAACAATGCT GGCTAAAGCA 
GTAGCTGCAG AGTCTAATGC GACCTTTTTC AACATAAGTG CTGCCAGTTT AACTTCAAAA 
TATGTGGGAG AAGGAGAGAA ATTGGTGAGA GCTCTCTTTG CTGTGGCTCG AGAACTTCAA 
CCATCTATAA TTTTTATAGA TGAAGTTGAC AGTCTTTTGT GTGAGAGACG GGAAGGGGAG 
CACGACGCTA GCAGACGGCT AAAGACGGAA TTTTTAATAG AATTTGACGG GGTGCAATCT 
GCTGGAGATG ACAGAGTACT TGTAATGGGT GCAACTAACA GGCCCCAAGA GCTTGATGAA 
GCTGTTCTCA GGCGTTTCAT TAAACGGGTA TATGTGTCCT TACCAAATGA GGAGACAAGA 
CTCCTTCTGC TTAAAAACCT GTTGTGTAAA CAAGGAAGTC CACTGACCCA AAAAGAACTC 
GCACAGCTTG CTAGAATGAC CGATGGATAC TCTGGAAGTG ATCTGACCGC TTTGGCCAAG 
GATGCAGCCC TGGGTCCTAT CCGAGAACTG AAGCCAGAGC AGGTGAAGAA TATGTCTGCC 
AGTGAGATGA GAAATATTCG ATTATCTGAC TTCACAGAAT CCTTAAAAAA GATAAAACGC 
AGTGTGAGTC CTCAGACCTT AGAAGCATAC ATACGCTGGA ACAAGGATTT TGGAGACACC 
ACTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524673.1
CDS	1115..2863
Translation	MSSPAGRRKKKGSGGASPAPARPPPPAAVPAPAAGPAPAAGSPPKRNPSSFSSPLVVGFALLRLLACHLGLLFAWLCQRFSRALMAAKRSSGTAPAPASPSPPEPGPGGEAESVRVFHKQAFEYISIALRIDEEEKAGQKEQAVEWYKKGIEELEKGIAVIVTGQGEQYERARRLQAKMMTNLVMAKDRLQLLESGAVPKRKDPLTHASNSLPRSKTVLKSGSAGLSGHHRAPSCSGLSMVSGARPGPGPAATTHKGTPKPNRTNKPSTPTTAVRKKKDLKNFRNVDSNLANLIMNEIVDNGTAVKFDDIAGQELAKQALQEIVILPSLRPELFTGLRAPARGLLLFGPPGNGKTMLAKAVAAESNATFFNISAASLTSKYVGEGEKLVRALFAVARELQPSIIFIDEVDSLLCERREGEHDASRRLKTEFLIEFDGVQSAGDDRVLVMGATNRPQELDEAVLRRFIKRVYVSLPNEETRLLLLKNLLCKQGSPLTQKELAQLARMTDGYSGSDLTALAKDAALGPIRELKPEQVKNMSASEMRNIRLSDFTESLKKIKRSVSPQTLEAYIRWNKDFGDTTV

Target ORF information:

RefSeq Version	XM_006524610.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus spastin (Spast), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524610.4

ORF Insert Sequence:

ATGAGTTCTC CGGCCGGACG ACGGAAGAAG AAAGGCTCGG GCGGCGCGAG CCCGGCGCCC 
GCCAGGCCTC CGCCCCCCGC CGCGGTCCCC GCCCCTGCCG CCGGCCCGGC CCCTGCGGCC 
GGCTCGCCGC CTAAGCGGAA CCCGTCTTCT TTCTCGTCCC CGCTGGTCGT CGGCTTCGCC 
CTGCTGCGCC TGCTGGCCTG CCACCTGGGG CTCCTCTTCG CGTGGCTCTG CCAGCGCTTC 
TCCCGCGCCC TCATGGCCGC CAAGAGGAGC TCCGGGACCG CGCCGGCGCC CGCCTCGCCC 
TCGCCCCCAG AGCCCGGACC GGGTGGCGAG GCCGAGAGCG TCCGCGTCTT CCACAAGCAG 
GCCTTCGAGT ACATCTCCAT TGCCCTGCGC ATCGACGAGG AAGAGAAAGC AGGACAGAAG 
GAACAAGCTG TGGAATGGTA TAAGAAAGGT ATCGAAGAAC TGGAAAAAGG AATCGCTGTT 
ATAGTTACGG GCCAAGGTGA ACAGTATGAA AGAGCTAGAC GTCTTCAAGC CAAAATGATG 
ACTAATTTAG TTATGGCCAA GGACCGTTTA CAACTTCTAG AAAGTGGAGC AGTTCCGAAG 
AGGAAAGACC CCTTAACACA TGCTAGTAAT TCATTGCCTC GATCAAAAAC TGTCCTGAAA 
AGTGGCTCCG CAGGGCTCTC CGGTCACCAC AGGGCGCCTA GTTGCAGTGG TTTGTCCATG 
GTTTCTGGAG CAAGACCGGG ACCTGGTCCT GCAGCTACCA CACATAAGGG TACTCCAAAA 
CCAAATAGAA CCAACAAACC TTCTACTCCC ACAACTGCAG TTCGGAAAAA GAAAGACTTG 
AAAAATTTTA GGAATGTGGA CAGCAATCTT GCTAACCTTA TAATGAATGA AATTGTTGAC 
AATGGGACAG CTGTTAAGTT TGATGACATA GCCGGGCAGG AGCTGGCAAA GCAAGCGCTG 
CAGGAGATTG TCATCCTTCC TTCTCTGCGG CCTGAGTTGT TCACAGGGCT CAGAGCTCCT 
GCTAGAGGCT TGTTACTCTT CGGTCCGCCA GGAAACGGAA AAACAATGCT GGCTAAAGCA 
GTAGCTGCAG AGTCTAATGC GACCTTTTTC AACATAAGTG CTGCCAGTTT AACTTCAAAA 
TATGTGGGAG AAGGAGAGAA ATTGGTGAGA GCTCTCTTTG CTGTGGCTCG AGAACTTCAA 
CCATCTATAA TTTTTATAGA TGAAGTTGAC AGTCTTTTGT GTGAGAGACG GGAAGGGGAG 
CACGACGCTA GCAGACGGCT AAAGACGGAA TTTTTAATAG AATTTGACGG GGTGCAATCT 
GCTGGAGATG ACAGAGTACT TGTAATGGGT GCAACTAACA GGCCCCAAGA GCTTGATGAA 
GCTGTTCTCA GGCGTTTCAT TAAACGGGTA TATGTGTCCT TACCAAATGA GGAGACAAGA 
CTCCTTCTGC TTAAAAACCT GTTGTGTAAA CAAGGAAGTC CACTGACCCA AAAAGAACTC 
GCACAGCTTG CTAGAATGAC CGATGGATAC TCTGGAAGTG ATCTGACCGC TTTGGCCAAG 
GATGCAGCCC TGGGTCCTAT CCGAGAACTG AAGCCAGAGC AGGTGAAGAA TATGTCTGCC 
AGTGAGATGA GAAATATTCG ATTATCTGAC TTCACAGAAT CCTTAAAAAA GATAAAACGC 
AGTGTGAGTC CTCAGACCTT AGAAGCATAC ATACGCTGGA ACAAGGATTT TGGAGACACC 
ACTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spast ( NM_001162870.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001156342.1 Mus musculus spastin (Spast), transcript variant 1, mRNA.

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CloneID	OMu08507
Clone ID Related Accession (Same CDS sequence)	NM_001162870.1
Accession Version	NM_001162870.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1845bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-21
Organism	Mus musculus(house mouse)
Product	spastin isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CF531661.1, CJ047532.1, AJ246002.1 and AC169506.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK129282.1, AJ246002.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTTCTC CGGCCGGACG ACGGAAGAAG AAAGGCTCGG GCGGCGCGAG CCCGGCGCCC 
GCCAGGCCTC CGCCCCCCGC CGCGGTCCCC GCCCCTGCCG CCGGCCCGGC CCCTGCGGCC 
GGCTCGCCGC CTAAGCGGAA CCCGTCTTCT TTCTCGTCCC CGCTGGTCGT CGGCTTCGCC 
CTGCTGCGCC TGCTGGCCTG CCACCTGGGG CTCCTCTTCG CGTGGCTCTG CCAGCGCTTC 
TCCCGCGCCC TCATGGCCGC CAAGAGGAGC TCCGGGACCG CGCCGGCGCC CGCCTCGCCC 
TCGCCCCCAG AGCCCGGACC GGGTGGCGAG GCCGAGAGCG TCCGCGTCTT CCACAAGCAG 
GCCTTCGAGT ACATCTCCAT TGCCCTGCGC ATCGACGAGG AAGAGAAAGC AGGACAGAAG 
GAACAAGCTG TGGAATGGTA TAAGAAAGGT ATCGAAGAAC TGGAAAAAGG AATCGCTGTT 
ATAGTTACGG GCCAAGGTGA ACAGTATGAA AGAGCTAGAC GTCTTCAAGC CAAAATGATG 
ACTAATTTAG TTATGGCCAA GGACCGTTTA CAACTTCTAG AGAAGCTGCA ACCAGTTTTG 
CAATTTTCCA AGTCACAGAC GGACGTCTAT AACGAGAGTA CTAACCTGAC ATGCCGCAAT 
GGACATCTCC AGTCAGAAAG TGGAGCAGTT CCGAAGAGGA AAGACCCCTT AACACATGCT 
AGTAATTCAT TGCCTCGATC AAAAACTGTC CTGAAAAGTG GCTCCGCAGG GCTCTCCGGT 
CACCACAGGG CGCCTAGTTG CAGTGGTTTG TCCATGGTTT CTGGAGCAAG ACCGGGACCT 
GGTCCTGCAG CTACCACACA TAAGGGTACT CCAAAACCAA ATAGAACCAA CAAACCTTCT 
ACTCCCACAA CTGCAGTTCG GAAAAAGAAA GACTTGAAAA ATTTTAGGAA TGTGGACAGC 
AATCTTGCTA ACCTTATAAT GAATGAAATT GTTGACAATG GGACAGCTGT TAAGTTTGAT 
GACATAGCCG GGCAGGAGCT GGCAAAGCAA GCGCTGCAGG AGATTGTCAT CCTTCCTTCT 
CTGCGGCCTG AGTTGTTCAC AGGGCTCAGA GCTCCTGCTA GAGGCTTGTT ACTCTTCGGT 
CCGCCAGGAA ACGGAAAAAC AATGCTGGCT AAAGCAGTAG CTGCAGAGTC TAATGCGACC 
TTTTTCAACA TAAGTGCTGC CAGTTTAACT TCAAAATATG TGGGAGAAGG AGAGAAATTG 
GTGAGAGCTC TCTTTGCTGT GGCTCGAGAA CTTCAACCAT CTATAATTTT TATAGATGAA 
GTTGACAGTC TTTTGTGTGA GAGACGGGAA GGGGAGCACG ACGCTAGCAG ACGGCTAAAG 
ACGGAATTTT TAATAGAATT TGACGGGGTG CAATCTGCTG GAGATGACAG AGTACTTGTA 
ATGGGTGCAA CTAACAGGCC CCAAGAGCTT GATGAAGCTG TTCTCAGGCG TTTCATTAAA 
CGGGTATATG TGTCCTTACC AAATGAGGAG ACAAGACTCC TTCTGCTTAA AAACCTGTTG 
TGTAAACAAG GAAGTCCACT GACCCAAAAA GAACTCGCAC AGCTTGCTAG AATGACCGAT 
GGATACTCTG GAAGTGATCT GACCGCTTTG GCCAAGGATG CAGCCCTGGG TCCTATCCGA 
GAACTGAAGC CAGAGCAGGT GAAGAATATG TCTGCCAGTG AGATGAGAAA TATTCGATTA 
TCTGACTTCA CAGAATCCTT AAAAAAGATA AAACGCAGTG TGAGTCCTCA GACCTTAGAA 
GCATACATAC GCTGGAACAA GGATTTTGGA GACACCACTG TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001156342.1
CDS	51..1895
Translation	MSSPAGRRKKKGSGGASPAPARPPPPAAVPAPAAGPAPAAGSPPKRNPSSFSSPLVVGFALLRLLACHLGLLFAWLCQRFSRALMAAKRSSGTAPAPASPSPPEPGPGGEAESVRVFHKQAFEYISIALRIDEEEKAGQKEQAVEWYKKGIEELEKGIAVIVTGQGEQYERARRLQAKMMTNLVMAKDRLQLLEKLQPVLQFSKSQTDVYNESTNLTCRNGHLQSESGAVPKRKDPLTHASNSLPRSKTVLKSGSAGLSGHHRAPSCSGLSMVSGARPGPGPAATTHKGTPKPNRTNKPSTPTTAVRKKKDLKNFRNVDSNLANLIMNEIVDNGTAVKFDDIAGQELAKQALQEIVILPSLRPELFTGLRAPARGLLLFGPPGNGKTMLAKAVAAESNATFFNISAASLTSKYVGEGEKLVRALFAVARELQPSIIFIDEVDSLLCERREGEHDASRRLKTEFLIEFDGVQSAGDDRVLVMGATNRPQELDEAVLRRFIKRVYVSLPNEETRLLLLKNLLCKQGSPLTQKELAQLARMTDGYSGSDLTALAKDAALGPIRELKPEQVKNMSASEMRNIRLSDFTESLKKIKRSVSPQTLEAYIRWNKDFGDTTV

Target ORF information:

RefSeq Version	NM_001162870.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus spastin (Spast), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001162870.1

ORF Insert Sequence:

ATGAGTTCTC CGGCCGGACG ACGGAAGAAG AAAGGCTCGG GCGGCGCGAG CCCGGCGCCC 
GCCAGGCCTC CGCCCCCCGC CGCGGTCCCC GCCCCTGCCG CCGGCCCGGC CCCTGCGGCC 
GGCTCGCCGC CTAAGCGGAA CCCGTCTTCT TTCTCGTCCC CGCTGGTCGT CGGCTTCGCC 
CTGCTGCGCC TGCTGGCCTG CCACCTGGGG CTCCTCTTCG CGTGGCTCTG CCAGCGCTTC 
TCCCGCGCCC TCATGGCCGC CAAGAGGAGC TCCGGGACCG CGCCGGCGCC CGCCTCGCCC 
TCGCCCCCAG AGCCCGGACC GGGTGGCGAG GCCGAGAGCG TCCGCGTCTT CCACAAGCAG 
GCCTTCGAGT ACATCTCCAT TGCCCTGCGC ATCGACGAGG AAGAGAAAGC AGGACAGAAG 
GAACAAGCTG TGGAATGGTA TAAGAAAGGT ATCGAAGAAC TGGAAAAAGG AATCGCTGTT 
ATAGTTACGG GCCAAGGTGA ACAGTATGAA AGAGCTAGAC GTCTTCAAGC CAAAATGATG 
ACTAATTTAG TTATGGCCAA GGACCGTTTA CAACTTCTAG AGAAGCTGCA ACCAGTTTTG 
CAATTTTCCA AGTCACAGAC GGACGTCTAT AACGAGAGTA CTAACCTGAC ATGCCGCAAT 
GGACATCTCC AGTCAGAAAG TGGAGCAGTT CCGAAGAGGA AAGACCCCTT AACACATGCT 
AGTAATTCAT TGCCTCGATC AAAAACTGTC CTGAAAAGTG GCTCCGCAGG GCTCTCCGGT 
CACCACAGGG CGCCTAGTTG CAGTGGTTTG TCCATGGTTT CTGGAGCAAG ACCGGGACCT 
GGTCCTGCAG CTACCACACA TAAGGGTACT CCAAAACCAA ATAGAACCAA CAAACCTTCT 
ACTCCCACAA CTGCAGTTCG GAAAAAGAAA GACTTGAAAA ATTTTAGGAA TGTGGACAGC 
AATCTTGCTA ACCTTATAAT GAATGAAATT GTTGACAATG GGACAGCTGT TAAGTTTGAT 
GACATAGCCG GGCAGGAGCT GGCAAAGCAA GCGCTGCAGG AGATTGTCAT CCTTCCTTCT 
CTGCGGCCTG AGTTGTTCAC AGGGCTCAGA GCTCCTGCTA GAGGCTTGTT ACTCTTCGGT 
CCGCCAGGAA ACGGAAAAAC AATGCTGGCT AAAGCAGTAG CTGCAGAGTC TAATGCGACC 
TTTTTCAACA TAAGTGCTGC CAGTTTAACT TCAAAATATG TGGGAGAAGG AGAGAAATTG 
GTGAGAGCTC TCTTTGCTGT GGCTCGAGAA CTTCAACCAT CTATAATTTT TATAGATGAA 
GTTGACAGTC TTTTGTGTGA GAGACGGGAA GGGGAGCACG ACGCTAGCAG ACGGCTAAAG 
ACGGAATTTT TAATAGAATT TGACGGGGTG CAATCTGCTG GAGATGACAG AGTACTTGTA 
ATGGGTGCAA CTAACAGGCC CCAAGAGCTT GATGAAGCTG TTCTCAGGCG TTTCATTAAA 
CGGGTATATG TGTCCTTACC AAATGAGGAG ACAAGACTCC TTCTGCTTAA AAACCTGTTG 
TGTAAACAAG GAAGTCCACT GACCCAAAAA GAACTCGCAC AGCTTGCTAG AATGACCGAT 
GGATACTCTG GAAGTGATCT GACCGCTTTG GCCAAGGATG CAGCCCTGGG TCCTATCCGA 
GAACTGAAGC CAGAGCAGGT GAAGAATATG TCTGCCAGTG AGATGAGAAA TATTCGATTA 
TCTGACTTCA CAGAATCCTT AAAAAAGATA AAACGCAGTG TGAGTCCTCA GACCTTAGAA 
GCATACATAC GCTGGAACAA GGATTTTGGA GACACCACTG TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spast ( NM_016962.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_058658.2 Mus musculus spastin (Spast), transcript variant 2, mRNA.

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CloneID	OMu10452
Clone ID Related Accession (Same CDS sequence)	NM_016962.2
Accession Version	NM_016962.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1842bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Mus musculus(house mouse)
Product	spastin isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CF531661.1, AK007793.1 and AC169506.1. On Jul 2, 2009 this sequence version replaced NM_016962.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region compared to variant 1. This results in a shorter protein (isoform 2) compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC046286.1, SRR1660813.4593.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTTCTC CGGCCGGACG ACGGAAGAAG AAAGGCTCGG GCGGCGCGAG CCCGGCGCCC 
GCCAGGCCTC CGCCCCCCGC CGCGGTCCCC GCCCCTGCCG CCGGCCCGGC CCCTGCGGCC 
GGCTCGCCGC CTAAGCGGAA CCCGTCTTCT TTCTCGTCCC CGCTGGTCGT CGGCTTCGCC 
CTGCTGCGCC TGCTGGCCTG CCACCTGGGG CTCCTCTTCG CGTGGCTCTG CCAGCGCTTC 
TCCCGCGCCC TCATGGCCGC CAAGAGGAGC TCCGGGACCG CGCCGGCGCC CGCCTCGCCC 
TCGCCCCCAG AGCCCGGACC GGGTGGCGAG GCCGAGAGCG TCCGCGTCTT CCACAAGCAG 
GCCTTCGAGT ACATCTCCAT TGCCCTGCGC ATCGACGAGG AAGAGAAAGG ACAGAAGGAA 
CAAGCTGTGG AATGGTATAA GAAAGGTATC GAAGAACTGG AAAAAGGAAT CGCTGTTATA 
GTTACGGGCC AAGGTGAACA GTATGAAAGA GCTAGACGTC TTCAAGCCAA AATGATGACT 
AATTTAGTTA TGGCCAAGGA CCGTTTACAA CTTCTAGAGA AGCTGCAACC AGTTTTGCAA 
TTTTCCAAGT CACAGACGGA CGTCTATAAC GAGAGTACTA ACCTGACATG CCGCAATGGA 
CATCTCCAGT CAGAAAGTGG AGCAGTTCCG AAGAGGAAAG ACCCCTTAAC ACATGCTAGT 
AATTCATTGC CTCGATCAAA AACTGTCCTG AAAAGTGGCT CCGCAGGGCT CTCCGGTCAC 
CACAGGGCGC CTAGTTGCAG TGGTTTGTCC ATGGTTTCTG GAGCAAGACC GGGACCTGGT 
CCTGCAGCTA CCACACATAA GGGTACTCCA AAACCAAATA GAACCAACAA ACCTTCTACT 
CCCACAACTG CAGTTCGGAA AAAGAAAGAC TTGAAAAATT TTAGGAATGT GGACAGCAAT 
CTTGCTAACC TTATAATGAA TGAAATTGTT GACAATGGGA CAGCTGTTAA GTTTGATGAC 
ATAGCCGGGC AGGAGCTGGC AAAGCAAGCG CTGCAGGAGA TTGTCATCCT TCCTTCTCTG 
CGGCCTGAGT TGTTCACAGG GCTCAGAGCT CCTGCTAGAG GCTTGTTACT CTTCGGTCCG 
CCAGGAAACG GAAAAACAAT GCTGGCTAAA GCAGTAGCTG CAGAGTCTAA TGCGACCTTT 
TTCAACATAA GTGCTGCCAG TTTAACTTCA AAATATGTGG GAGAAGGAGA GAAATTGGTG 
AGAGCTCTCT TTGCTGTGGC TCGAGAACTT CAACCATCTA TAATTTTTAT AGATGAAGTT 
GACAGTCTTT TGTGTGAGAG ACGGGAAGGG GAGCACGACG CTAGCAGACG GCTAAAGACG 
GAATTTTTAA TAGAATTTGA CGGGGTGCAA TCTGCTGGAG ATGACAGAGT ACTTGTAATG 
GGTGCAACTA ACAGGCCCCA AGAGCTTGAT GAAGCTGTTC TCAGGCGTTT CATTAAACGG 
GTATATGTGT CCTTACCAAA TGAGGAGACA AGACTCCTTC TGCTTAAAAA CCTGTTGTGT 
AAACAAGGAA GTCCACTGAC CCAAAAAGAA CTCGCACAGC TTGCTAGAAT GACCGATGGA 
TACTCTGGAA GTGATCTGAC CGCTTTGGCC AAGGATGCAG CCCTGGGTCC TATCCGAGAA 
CTGAAGCCAG AGCAGGTGAA GAATATGTCT GCCAGTGAGA TGAGAAATAT TCGATTATCT 
GACTTCACAG AATCCTTAAA AAAGATAAAA CGCAGTGTGA GTCCTCAGAC CTTAGAAGCA 
TACATACGCT GGAACAAGGA TTTTGGAGAC ACCACTGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058658.2
CDS	51..1892
Translation	MSSPAGRRKKKGSGGASPAPARPPPPAAVPAPAAGPAPAAGSPPKRNPSSFSSPLVVGFALLRLLACHLGLLFAWLCQRFSRALMAAKRSSGTAPAPASPSPPEPGPGGEAESVRVFHKQAFEYISIALRIDEEEKGQKEQAVEWYKKGIEELEKGIAVIVTGQGEQYERARRLQAKMMTNLVMAKDRLQLLEKLQPVLQFSKSQTDVYNESTNLTCRNGHLQSESGAVPKRKDPLTHASNSLPRSKTVLKSGSAGLSGHHRAPSCSGLSMVSGARPGPGPAATTHKGTPKPNRTNKPSTPTTAVRKKKDLKNFRNVDSNLANLIMNEIVDNGTAVKFDDIAGQELAKQALQEIVILPSLRPELFTGLRAPARGLLLFGPPGNGKTMLAKAVAAESNATFFNISAASLTSKYVGEGEKLVRALFAVARELQPSIIFIDEVDSLLCERREGEHDASRRLKTEFLIEFDGVQSAGDDRVLVMGATNRPQELDEAVLRRFIKRVYVSLPNEETRLLLLKNLLCKQGSPLTQKELAQLARMTDGYSGSDLTALAKDAALGPIRELKPEQVKNMSASEMRNIRLSDFTESLKKIKRSVSPQTLEAYIRWNKDFGDTTV

Target ORF information:

RefSeq Version	NM_016962.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus spastin (Spast), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016962.2

ORF Insert Sequence:

ATGAGTTCTC CGGCCGGACG ACGGAAGAAG AAAGGCTCGG GCGGCGCGAG CCCGGCGCCC 
GCCAGGCCTC CGCCCCCCGC CGCGGTCCCC GCCCCTGCCG CCGGCCCGGC CCCTGCGGCC 
GGCTCGCCGC CTAAGCGGAA CCCGTCTTCT TTCTCGTCCC CGCTGGTCGT CGGCTTCGCC 
CTGCTGCGCC TGCTGGCCTG CCACCTGGGG CTCCTCTTCG CGTGGCTCTG CCAGCGCTTC 
TCCCGCGCCC TCATGGCCGC CAAGAGGAGC TCCGGGACCG CGCCGGCGCC CGCCTCGCCC 
TCGCCCCCAG AGCCCGGACC GGGTGGCGAG GCCGAGAGCG TCCGCGTCTT CCACAAGCAG 
GCCTTCGAGT ACATCTCCAT TGCCCTGCGC ATCGACGAGG AAGAGAAAGG ACAGAAGGAA 
CAAGCTGTGG AATGGTATAA GAAAGGTATC GAAGAACTGG AAAAAGGAAT CGCTGTTATA 
GTTACGGGCC AAGGTGAACA GTATGAAAGA GCTAGACGTC TTCAAGCCAA AATGATGACT 
AATTTAGTTA TGGCCAAGGA CCGTTTACAA CTTCTAGAGA AGCTGCAACC AGTTTTGCAA 
TTTTCCAAGT CACAGACGGA CGTCTATAAC GAGAGTACTA ACCTGACATG CCGCAATGGA 
CATCTCCAGT CAGAAAGTGG AGCAGTTCCG AAGAGGAAAG ACCCCTTAAC ACATGCTAGT 
AATTCATTGC CTCGATCAAA AACTGTCCTG AAAAGTGGCT CCGCAGGGCT CTCCGGTCAC 
CACAGGGCGC CTAGTTGCAG TGGTTTGTCC ATGGTTTCTG GAGCAAGACC GGGACCTGGT 
CCTGCAGCTA CCACACATAA GGGTACTCCA AAACCAAATA GAACCAACAA ACCTTCTACT 
CCCACAACTG CAGTTCGGAA AAAGAAAGAC TTGAAAAATT TTAGGAATGT GGACAGCAAT 
CTTGCTAACC TTATAATGAA TGAAATTGTT GACAATGGGA CAGCTGTTAA GTTTGATGAC 
ATAGCCGGGC AGGAGCTGGC AAAGCAAGCG CTGCAGGAGA TTGTCATCCT TCCTTCTCTG 
CGGCCTGAGT TGTTCACAGG GCTCAGAGCT CCTGCTAGAG GCTTGTTACT CTTCGGTCCG 
CCAGGAAACG GAAAAACAAT GCTGGCTAAA GCAGTAGCTG CAGAGTCTAA TGCGACCTTT 
TTCAACATAA GTGCTGCCAG TTTAACTTCA AAATATGTGG GAGAAGGAGA GAAATTGGTG 
AGAGCTCTCT TTGCTGTGGC TCGAGAACTT CAACCATCTA TAATTTTTAT AGATGAAGTT 
GACAGTCTTT TGTGTGAGAG ACGGGAAGGG GAGCACGACG CTAGCAGACG GCTAAAGACG 
GAATTTTTAA TAGAATTTGA CGGGGTGCAA TCTGCTGGAG ATGACAGAGT ACTTGTAATG 
GGTGCAACTA ACAGGCCCCA AGAGCTTGAT GAAGCTGTTC TCAGGCGTTT CATTAAACGG 
GTATATGTGT CCTTACCAAA TGAGGAGACA AGACTCCTTC TGCTTAAAAA CCTGTTGTGT 
AAACAAGGAA GTCCACTGAC CCAAAAAGAA CTCGCACAGC TTGCTAGAAT GACCGATGGA 
TACTCTGGAA GTGATCTGAC CGCTTTGGCC AAGGATGCAG CCCTGGGTCC TATCCGAGAA 
CTGAAGCCAG AGCAGGTGAA GAATATGTCT GCCAGTGAGA TGAGAAATAT TCGATTATCT 
GACTTCACAG AATCCTTAAA AAAGATAAAA CGCAGTGTGA GTCCTCAGAC CTTAGAAGCA 
TACATACGCT GGAACAAGGA TTTTGGAGAC ACCACTGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spast ( NM_001357738.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001344667.1 Mus musculus spastin (Spast), transcript variant 3, mRNA.

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CloneID	OMu53828
Clone ID Related Accession (Same CDS sequence)	NM_001357738.1 , XM_006524611.3
Accession Version	NM_001357738.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1746bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Mus musculus(house mouse)
Product	spastin isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC169506.1 and AI666403.1. On Nov 4, 2017 this sequence version replaced XM_006524611.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660813.77921.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTTCTC CGGCCGGACG ACGGAAGAAG AAAGGCTCGG GCGGCGCGAG CCCGGCGCCC 
GCCAGGCCTC CGCCCCCCGC CGCGGTCCCC GCCCCTGCCG CCGGCCCGGC CCCTGCGGCC 
GGCTCGCCGC CTAAGCGGAA CCCGTCTTCT TTCTCGTCCC CGCTGGTCGT CGGCTTCGCC 
CTGCTGCGCC TGCTGGCCTG CCACCTGGGG CTCCTCTTCG CGTGGCTCTG CCAGCGCTTC 
TCCCGCGCCC TCATGGCCGC CAAGAGGAGC TCCGGGACCG CGCCGGCGCC CGCCTCGCCC 
TCGCCCCCAG AGCCCGGACC GGGTGGCGAG GCCGAGAGCG TCCGCGTCTT CCACAAGCAG 
GCCTTCGAGT ACATCTCCAT TGCCCTGCGC ATCGACGAGG AAGAGAAAGG ACAGAAGGAA 
CAAGCTGTGG AATGGTATAA GAAAGGTATC GAAGAACTGG AAAAAGGAAT CGCTGTTATA 
GTTACGGGCC AAGGTGAACA GTATGAAAGA GCTAGACGTC TTCAAGCCAA AATGATGACT 
AATTTAGTTA TGGCCAAGGA CCGTTTACAA CTTCTAGAAA GTGGAGCAGT TCCGAAGAGG 
AAAGACCCCT TAACACATGC TAGTAATTCA TTGCCTCGAT CAAAAACTGT CCTGAAAAGT 
GGCTCCGCAG GGCTCTCCGG TCACCACAGG GCGCCTAGTT GCAGTGGTTT GTCCATGGTT 
TCTGGAGCAA GACCGGGACC TGGTCCTGCA GCTACCACAC ATAAGGGTAC TCCAAAACCA 
AATAGAACCA ACAAACCTTC TACTCCCACA ACTGCAGTTC GGAAAAAGAA AGACTTGAAA 
AATTTTAGGA ATGTGGACAG CAATCTTGCT AACCTTATAA TGAATGAAAT TGTTGACAAT 
GGGACAGCTG TTAAGTTTGA TGACATAGCC GGGCAGGAGC TGGCAAAGCA AGCGCTGCAG 
GAGATTGTCA TCCTTCCTTC TCTGCGGCCT GAGTTGTTCA CAGGGCTCAG AGCTCCTGCT 
AGAGGCTTGT TACTCTTCGG TCCGCCAGGA AACGGAAAAA CAATGCTGGC TAAAGCAGTA 
GCTGCAGAGT CTAATGCGAC CTTTTTCAAC ATAAGTGCTG CCAGTTTAAC TTCAAAATAT 
GTGGGAGAAG GAGAGAAATT GGTGAGAGCT CTCTTTGCTG TGGCTCGAGA ACTTCAACCA 
TCTATAATTT TTATAGATGA AGTTGACAGT CTTTTGTGTG AGAGACGGGA AGGGGAGCAC 
GACGCTAGCA GACGGCTAAA GACGGAATTT TTAATAGAAT TTGACGGGGT GCAATCTGCT 
GGAGATGACA GAGTACTTGT AATGGGTGCA ACTAACAGGC CCCAAGAGCT TGATGAAGCT 
GTTCTCAGGC GTTTCATTAA ACGGGTATAT GTGTCCTTAC CAAATGAGGA GACAAGACTC 
CTTCTGCTTA AAAACCTGTT GTGTAAACAA GGAAGTCCAC TGACCCAAAA AGAACTCGCA 
CAGCTTGCTA GAATGACCGA TGGATACTCT GGAAGTGATC TGACCGCTTT GGCCAAGGAT 
GCAGCCCTGG GTCCTATCCG AGAACTGAAG CCAGAGCAGG TGAAGAATAT GTCTGCCAGT 
GAGATGAGAA ATATTCGATT ATCTGACTTC ACAGAATCCT TAAAAAAGAT AAAACGCAGT 
GTGAGTCCTC AGACCTTAGA AGCATACATA CGCTGGAACA AGGATTTTGG AGACACCACT 
GTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001344667.1
CDS	51..1796
Translation	MSSPAGRRKKKGSGGASPAPARPPPPAAVPAPAAGPAPAAGSPPKRNPSSFSSPLVVGFALLRLLACHLGLLFAWLCQRFSRALMAAKRSSGTAPAPASPSPPEPGPGGEAESVRVFHKQAFEYISIALRIDEEEKGQKEQAVEWYKKGIEELEKGIAVIVTGQGEQYERARRLQAKMMTNLVMAKDRLQLLESGAVPKRKDPLTHASNSLPRSKTVLKSGSAGLSGHHRAPSCSGLSMVSGARPGPGPAATTHKGTPKPNRTNKPSTPTTAVRKKKDLKNFRNVDSNLANLIMNEIVDNGTAVKFDDIAGQELAKQALQEIVILPSLRPELFTGLRAPARGLLLFGPPGNGKTMLAKAVAAESNATFFNISAASLTSKYVGEGEKLVRALFAVARELQPSIIFIDEVDSLLCERREGEHDASRRLKTEFLIEFDGVQSAGDDRVLVMGATNRPQELDEAVLRRFIKRVYVSLPNEETRLLLLKNLLCKQGSPLTQKELAQLARMTDGYSGSDLTALAKDAALGPIRELKPEQVKNMSASEMRNIRLSDFTESLKKIKRSVSPQTLEAYIRWNKDFGDTTV

Target ORF information:

RefSeq Version	NM_001357738.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus spastin (Spast), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001357738.1

ORF Insert Sequence:

ATGAGTTCTC CGGCCGGACG ACGGAAGAAG AAAGGCTCGG GCGGCGCGAG CCCGGCGCCC 
GCCAGGCCTC CGCCCCCCGC CGCGGTCCCC GCCCCTGCCG CCGGCCCGGC CCCTGCGGCC 
GGCTCGCCGC CTAAGCGGAA CCCGTCTTCT TTCTCGTCCC CGCTGGTCGT CGGCTTCGCC 
CTGCTGCGCC TGCTGGCCTG CCACCTGGGG CTCCTCTTCG CGTGGCTCTG CCAGCGCTTC 
TCCCGCGCCC TCATGGCCGC CAAGAGGAGC TCCGGGACCG CGCCGGCGCC CGCCTCGCCC 
TCGCCCCCAG AGCCCGGACC GGGTGGCGAG GCCGAGAGCG TCCGCGTCTT CCACAAGCAG 
GCCTTCGAGT ACATCTCCAT TGCCCTGCGC ATCGACGAGG AAGAGAAAGG ACAGAAGGAA 
CAAGCTGTGG AATGGTATAA GAAAGGTATC GAAGAACTGG AAAAAGGAAT CGCTGTTATA 
GTTACGGGCC AAGGTGAACA GTATGAAAGA GCTAGACGTC TTCAAGCCAA AATGATGACT 
AATTTAGTTA TGGCCAAGGA CCGTTTACAA CTTCTAGAAA GTGGAGCAGT TCCGAAGAGG 
AAAGACCCCT TAACACATGC TAGTAATTCA TTGCCTCGAT CAAAAACTGT CCTGAAAAGT 
GGCTCCGCAG GGCTCTCCGG TCACCACAGG GCGCCTAGTT GCAGTGGTTT GTCCATGGTT 
TCTGGAGCAA GACCGGGACC TGGTCCTGCA GCTACCACAC ATAAGGGTAC TCCAAAACCA 
AATAGAACCA ACAAACCTTC TACTCCCACA ACTGCAGTTC GGAAAAAGAA AGACTTGAAA 
AATTTTAGGA ATGTGGACAG CAATCTTGCT AACCTTATAA TGAATGAAAT TGTTGACAAT 
GGGACAGCTG TTAAGTTTGA TGACATAGCC GGGCAGGAGC TGGCAAAGCA AGCGCTGCAG 
GAGATTGTCA TCCTTCCTTC TCTGCGGCCT GAGTTGTTCA CAGGGCTCAG AGCTCCTGCT 
AGAGGCTTGT TACTCTTCGG TCCGCCAGGA AACGGAAAAA CAATGCTGGC TAAAGCAGTA 
GCTGCAGAGT CTAATGCGAC CTTTTTCAAC ATAAGTGCTG CCAGTTTAAC TTCAAAATAT 
GTGGGAGAAG GAGAGAAATT GGTGAGAGCT CTCTTTGCTG TGGCTCGAGA ACTTCAACCA 
TCTATAATTT TTATAGATGA AGTTGACAGT CTTTTGTGTG AGAGACGGGA AGGGGAGCAC 
GACGCTAGCA GACGGCTAAA GACGGAATTT TTAATAGAAT TTGACGGGGT GCAATCTGCT 
GGAGATGACA GAGTACTTGT AATGGGTGCA ACTAACAGGC CCCAAGAGCT TGATGAAGCT 
GTTCTCAGGC GTTTCATTAA ACGGGTATAT GTGTCCTTAC CAAATGAGGA GACAAGACTC 
CTTCTGCTTA AAAACCTGTT GTGTAAACAA GGAAGTCCAC TGACCCAAAA AGAACTCGCA 
CAGCTTGCTA GAATGACCGA TGGATACTCT GGAAGTGATC TGACCGCTTT GGCCAAGGAT 
GCAGCCCTGG GTCCTATCCG AGAACTGAAG CCAGAGCAGG TGAAGAATAT GTCTGCCAGT 
GAGATGAGAA ATATTCGATT ATCTGACTTC ACAGAATCCT TAAAAAAGAT AAAACGCAGT 
GTGAGTCCTC AGACCTTAGA AGCATACATA CGCTGGAACA AGGATTTTGG AGACACCACT 
GTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spast ( XM_006524610.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524673.1 Mus musculus spastin (Spast), transcript variant X1, mRNA.

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CloneID	OMu53827
Clone ID Related Accession (Same CDS sequence)	XM_006524610.3 , XM_006524610.4
Accession Version	XM_006524610.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1749bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	spastin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006524610.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTCTC CGGCCGGACG ACGGAAGAAG AAAGGCTCGG GCGGCGCGAG CCCGGCGCCC 
GCCAGGCCTC CGCCCCCCGC CGCGGTCCCC GCCCCTGCCG CCGGCCCGGC CCCTGCGGCC 
GGCTCGCCGC CTAAGCGGAA CCCGTCTTCT TTCTCGTCCC CGCTGGTCGT CGGCTTCGCC 
CTGCTGCGCC TGCTGGCCTG CCACCTGGGG CTCCTCTTCG CGTGGCTCTG CCAGCGCTTC 
TCCCGCGCCC TCATGGCCGC CAAGAGGAGC TCCGGGACCG CGCCGGCGCC CGCCTCGCCC 
TCGCCCCCAG AGCCCGGACC GGGTGGCGAG GCCGAGAGCG TCCGCGTCTT CCACAAGCAG 
GCCTTCGAGT ACATCTCCAT TGCCCTGCGC ATCGACGAGG AAGAGAAAGC AGGACAGAAG 
GAACAAGCTG TGGAATGGTA TAAGAAAGGT ATCGAAGAAC TGGAAAAAGG AATCGCTGTT 
ATAGTTACGG GCCAAGGTGA ACAGTATGAA AGAGCTAGAC GTCTTCAAGC CAAAATGATG 
ACTAATTTAG TTATGGCCAA GGACCGTTTA CAACTTCTAG AAAGTGGAGC AGTTCCGAAG 
AGGAAAGACC CCTTAACACA TGCTAGTAAT TCATTGCCTC GATCAAAAAC TGTCCTGAAA 
AGTGGCTCCG CAGGGCTCTC CGGTCACCAC AGGGCGCCTA GTTGCAGTGG TTTGTCCATG 
GTTTCTGGAG CAAGACCGGG ACCTGGTCCT GCAGCTACCA CACATAAGGG TACTCCAAAA 
CCAAATAGAA CCAACAAACC TTCTACTCCC ACAACTGCAG TTCGGAAAAA GAAAGACTTG 
AAAAATTTTA GGAATGTGGA CAGCAATCTT GCTAACCTTA TAATGAATGA AATTGTTGAC 
AATGGGACAG CTGTTAAGTT TGATGACATA GCCGGGCAGG AGCTGGCAAA GCAAGCGCTG 
CAGGAGATTG TCATCCTTCC TTCTCTGCGG CCTGAGTTGT TCACAGGGCT CAGAGCTCCT 
GCTAGAGGCT TGTTACTCTT CGGTCCGCCA GGAAACGGAA AAACAATGCT GGCTAAAGCA 
GTAGCTGCAG AGTCTAATGC GACCTTTTTC AACATAAGTG CTGCCAGTTT AACTTCAAAA 
TATGTGGGAG AAGGAGAGAA ATTGGTGAGA GCTCTCTTTG CTGTGGCTCG AGAACTTCAA 
CCATCTATAA TTTTTATAGA TGAAGTTGAC AGTCTTTTGT GTGAGAGACG GGAAGGGGAG 
CACGACGCTA GCAGACGGCT AAAGACGGAA TTTTTAATAG AATTTGACGG GGTGCAATCT 
GCTGGAGATG ACAGAGTACT TGTAATGGGT GCAACTAACA GGCCCCAAGA GCTTGATGAA 
GCTGTTCTCA GGCGTTTCAT TAAACGGGTA TATGTGTCCT TACCAAATGA GGAGACAAGA 
CTCCTTCTGC TTAAAAACCT GTTGTGTAAA CAAGGAAGTC CACTGACCCA AAAAGAACTC 
GCACAGCTTG CTAGAATGAC CGATGGATAC TCTGGAAGTG ATCTGACCGC TTTGGCCAAG 
GATGCAGCCC TGGGTCCTAT CCGAGAACTG AAGCCAGAGC AGGTGAAGAA TATGTCTGCC 
AGTGAGATGA GAAATATTCG ATTATCTGAC TTCACAGAAT CCTTAAAAAA GATAAAACGC 
AGTGTGAGTC CTCAGACCTT AGAAGCATAC ATACGCTGGA ACAAGGATTT TGGAGACACC 
ACTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524673.1
CDS	1581..3329
Translation	MSSPAGRRKKKGSGGASPAPARPPPPAAVPAPAAGPAPAAGSPPKRNPSSFSSPLVVGFALLRLLACHLGLLFAWLCQRFSRALMAAKRSSGTAPAPASPSPPEPGPGGEAESVRVFHKQAFEYISIALRIDEEEKAGQKEQAVEWYKKGIEELEKGIAVIVTGQGEQYERARRLQAKMMTNLVMAKDRLQLLESGAVPKRKDPLTHASNSLPRSKTVLKSGSAGLSGHHRAPSCSGLSMVSGARPGPGPAATTHKGTPKPNRTNKPSTPTTAVRKKKDLKNFRNVDSNLANLIMNEIVDNGTAVKFDDIAGQELAKQALQEIVILPSLRPELFTGLRAPARGLLLFGPPGNGKTMLAKAVAAESNATFFNISAASLTSKYVGEGEKLVRALFAVARELQPSIIFIDEVDSLLCERREGEHDASRRLKTEFLIEFDGVQSAGDDRVLVMGATNRPQELDEAVLRRFIKRVYVSLPNEETRLLLLKNLLCKQGSPLTQKELAQLARMTDGYSGSDLTALAKDAALGPIRELKPEQVKNMSASEMRNIRLSDFTESLKKIKRSVSPQTLEAYIRWNKDFGDTTV

Target ORF information:

RefSeq Version	XM_006524610.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus spastin (Spast), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524610.3

ORF Insert Sequence:

ATGAGTTCTC CGGCCGGACG ACGGAAGAAG AAAGGCTCGG GCGGCGCGAG CCCGGCGCCC 
GCCAGGCCTC CGCCCCCCGC CGCGGTCCCC GCCCCTGCCG CCGGCCCGGC CCCTGCGGCC 
GGCTCGCCGC CTAAGCGGAA CCCGTCTTCT TTCTCGTCCC CGCTGGTCGT CGGCTTCGCC 
CTGCTGCGCC TGCTGGCCTG CCACCTGGGG CTCCTCTTCG CGTGGCTCTG CCAGCGCTTC 
TCCCGCGCCC TCATGGCCGC CAAGAGGAGC TCCGGGACCG CGCCGGCGCC CGCCTCGCCC 
TCGCCCCCAG AGCCCGGACC GGGTGGCGAG GCCGAGAGCG TCCGCGTCTT CCACAAGCAG 
GCCTTCGAGT ACATCTCCAT TGCCCTGCGC ATCGACGAGG AAGAGAAAGC AGGACAGAAG 
GAACAAGCTG TGGAATGGTA TAAGAAAGGT ATCGAAGAAC TGGAAAAAGG AATCGCTGTT 
ATAGTTACGG GCCAAGGTGA ACAGTATGAA AGAGCTAGAC GTCTTCAAGC CAAAATGATG 
ACTAATTTAG TTATGGCCAA GGACCGTTTA CAACTTCTAG AAAGTGGAGC AGTTCCGAAG 
AGGAAAGACC CCTTAACACA TGCTAGTAAT TCATTGCCTC GATCAAAAAC TGTCCTGAAA 
AGTGGCTCCG CAGGGCTCTC CGGTCACCAC AGGGCGCCTA GTTGCAGTGG TTTGTCCATG 
GTTTCTGGAG CAAGACCGGG ACCTGGTCCT GCAGCTACCA CACATAAGGG TACTCCAAAA 
CCAAATAGAA CCAACAAACC TTCTACTCCC ACAACTGCAG TTCGGAAAAA GAAAGACTTG 
AAAAATTTTA GGAATGTGGA CAGCAATCTT GCTAACCTTA TAATGAATGA AATTGTTGAC 
AATGGGACAG CTGTTAAGTT TGATGACATA GCCGGGCAGG AGCTGGCAAA GCAAGCGCTG 
CAGGAGATTG TCATCCTTCC TTCTCTGCGG CCTGAGTTGT TCACAGGGCT CAGAGCTCCT 
GCTAGAGGCT TGTTACTCTT CGGTCCGCCA GGAAACGGAA AAACAATGCT GGCTAAAGCA 
GTAGCTGCAG AGTCTAATGC GACCTTTTTC AACATAAGTG CTGCCAGTTT AACTTCAAAA 
TATGTGGGAG AAGGAGAGAA ATTGGTGAGA GCTCTCTTTG CTGTGGCTCG AGAACTTCAA 
CCATCTATAA TTTTTATAGA TGAAGTTGAC AGTCTTTTGT GTGAGAGACG GGAAGGGGAG 
CACGACGCTA GCAGACGGCT AAAGACGGAA TTTTTAATAG AATTTGACGG GGTGCAATCT 
GCTGGAGATG ACAGAGTACT TGTAATGGGT GCAACTAACA GGCCCCAAGA GCTTGATGAA 
GCTGTTCTCA GGCGTTTCAT TAAACGGGTA TATGTGTCCT TACCAAATGA GGAGACAAGA 
CTCCTTCTGC TTAAAAACCT GTTGTGTAAA CAAGGAAGTC CACTGACCCA AAAAGAACTC 
GCACAGCTTG CTAGAATGAC CGATGGATAC TCTGGAAGTG ATCTGACCGC TTTGGCCAAG 
GATGCAGCCC TGGGTCCTAT CCGAGAACTG AAGCCAGAGC AGGTGAAGAA TATGTCTGCC 
AGTGAGATGA GAAATATTCG ATTATCTGAC TTCACAGAAT CCTTAAAAAA GATAAAACGC 
AGTGTGAGTC CTCAGACCTT AGAAGCATAC ATACGCTGGA ACAAGGATTT TGGAGACACC 
ACTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spast ( XM_006524611.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524674.1 Mus musculus spastin (Spast), transcript variant X2, mRNA.

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CloneID	OMu53828
Clone ID Related Accession (Same CDS sequence)	NM_001357738.1 , XM_006524611.3
Accession Version	XM_006524611.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1746bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	spastin isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006524611.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTCTC CGGCCGGACG ACGGAAGAAG AAAGGCTCGG GCGGCGCGAG CCCGGCGCCC 
GCCAGGCCTC CGCCCCCCGC CGCGGTCCCC GCCCCTGCCG CCGGCCCGGC CCCTGCGGCC 
GGCTCGCCGC CTAAGCGGAA CCCGTCTTCT TTCTCGTCCC CGCTGGTCGT CGGCTTCGCC 
CTGCTGCGCC TGCTGGCCTG CCACCTGGGG CTCCTCTTCG CGTGGCTCTG CCAGCGCTTC 
TCCCGCGCCC TCATGGCCGC CAAGAGGAGC TCCGGGACCG CGCCGGCGCC CGCCTCGCCC 
TCGCCCCCAG AGCCCGGACC GGGTGGCGAG GCCGAGAGCG TCCGCGTCTT CCACAAGCAG 
GCCTTCGAGT ACATCTCCAT TGCCCTGCGC ATCGACGAGG AAGAGAAAGG ACAGAAGGAA 
CAAGCTGTGG AATGGTATAA GAAAGGTATC GAAGAACTGG AAAAAGGAAT CGCTGTTATA 
GTTACGGGCC AAGGTGAACA GTATGAAAGA GCTAGACGTC TTCAAGCCAA AATGATGACT 
AATTTAGTTA TGGCCAAGGA CCGTTTACAA CTTCTAGAAA GTGGAGCAGT TCCGAAGAGG 
AAAGACCCCT TAACACATGC TAGTAATTCA TTGCCTCGAT CAAAAACTGT CCTGAAAAGT 
GGCTCCGCAG GGCTCTCCGG TCACCACAGG GCGCCTAGTT GCAGTGGTTT GTCCATGGTT 
TCTGGAGCAA GACCGGGACC TGGTCCTGCA GCTACCACAC ATAAGGGTAC TCCAAAACCA 
AATAGAACCA ACAAACCTTC TACTCCCACA ACTGCAGTTC GGAAAAAGAA AGACTTGAAA 
AATTTTAGGA ATGTGGACAG CAATCTTGCT AACCTTATAA TGAATGAAAT TGTTGACAAT 
GGGACAGCTG TTAAGTTTGA TGACATAGCC GGGCAGGAGC TGGCAAAGCA AGCGCTGCAG 
GAGATTGTCA TCCTTCCTTC TCTGCGGCCT GAGTTGTTCA CAGGGCTCAG AGCTCCTGCT 
AGAGGCTTGT TACTCTTCGG TCCGCCAGGA AACGGAAAAA CAATGCTGGC TAAAGCAGTA 
GCTGCAGAGT CTAATGCGAC CTTTTTCAAC ATAAGTGCTG CCAGTTTAAC TTCAAAATAT 
GTGGGAGAAG GAGAGAAATT GGTGAGAGCT CTCTTTGCTG TGGCTCGAGA ACTTCAACCA 
TCTATAATTT TTATAGATGA AGTTGACAGT CTTTTGTGTG AGAGACGGGA AGGGGAGCAC 
GACGCTAGCA GACGGCTAAA GACGGAATTT TTAATAGAAT TTGACGGGGT GCAATCTGCT 
GGAGATGACA GAGTACTTGT AATGGGTGCA ACTAACAGGC CCCAAGAGCT TGATGAAGCT 
GTTCTCAGGC GTTTCATTAA ACGGGTATAT GTGTCCTTAC CAAATGAGGA GACAAGACTC 
CTTCTGCTTA AAAACCTGTT GTGTAAACAA GGAAGTCCAC TGACCCAAAA AGAACTCGCA 
CAGCTTGCTA GAATGACCGA TGGATACTCT GGAAGTGATC TGACCGCTTT GGCCAAGGAT 
GCAGCCCTGG GTCCTATCCG AGAACTGAAG CCAGAGCAGG TGAAGAATAT GTCTGCCAGT 
GAGATGAGAA ATATTCGATT ATCTGACTTC ACAGAATCCT TAAAAAAGAT AAAACGCAGT 
GTGAGTCCTC AGACCTTAGA AGCATACATA CGCTGGAACA AGGATTTTGG AGACACCACT 
GTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524674.1
CDS	1580..3325
Translation	MSSPAGRRKKKGSGGASPAPARPPPPAAVPAPAAGPAPAAGSPPKRNPSSFSSPLVVGFALLRLLACHLGLLFAWLCQRFSRALMAAKRSSGTAPAPASPSPPEPGPGGEAESVRVFHKQAFEYISIALRIDEEEKGQKEQAVEWYKKGIEELEKGIAVIVTGQGEQYERARRLQAKMMTNLVMAKDRLQLLESGAVPKRKDPLTHASNSLPRSKTVLKSGSAGLSGHHRAPSCSGLSMVSGARPGPGPAATTHKGTPKPNRTNKPSTPTTAVRKKKDLKNFRNVDSNLANLIMNEIVDNGTAVKFDDIAGQELAKQALQEIVILPSLRPELFTGLRAPARGLLLFGPPGNGKTMLAKAVAAESNATFFNISAASLTSKYVGEGEKLVRALFAVARELQPSIIFIDEVDSLLCERREGEHDASRRLKTEFLIEFDGVQSAGDDRVLVMGATNRPQELDEAVLRRFIKRVYVSLPNEETRLLLLKNLLCKQGSPLTQKELAQLARMTDGYSGSDLTALAKDAALGPIRELKPEQVKNMSASEMRNIRLSDFTESLKKIKRSVSPQTLEAYIRWNKDFGDTTV

Target ORF information:

RefSeq Version	XM_006524611.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus spastin (Spast), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524611.3

ORF Insert Sequence:

ATGAGTTCTC CGGCCGGACG ACGGAAGAAG AAAGGCTCGG GCGGCGCGAG CCCGGCGCCC 
GCCAGGCCTC CGCCCCCCGC CGCGGTCCCC GCCCCTGCCG CCGGCCCGGC CCCTGCGGCC 
GGCTCGCCGC CTAAGCGGAA CCCGTCTTCT TTCTCGTCCC CGCTGGTCGT CGGCTTCGCC 
CTGCTGCGCC TGCTGGCCTG CCACCTGGGG CTCCTCTTCG CGTGGCTCTG CCAGCGCTTC 
TCCCGCGCCC TCATGGCCGC CAAGAGGAGC TCCGGGACCG CGCCGGCGCC CGCCTCGCCC 
TCGCCCCCAG AGCCCGGACC GGGTGGCGAG GCCGAGAGCG TCCGCGTCTT CCACAAGCAG 
GCCTTCGAGT ACATCTCCAT TGCCCTGCGC ATCGACGAGG AAGAGAAAGG ACAGAAGGAA 
CAAGCTGTGG AATGGTATAA GAAAGGTATC GAAGAACTGG AAAAAGGAAT CGCTGTTATA 
GTTACGGGCC AAGGTGAACA GTATGAAAGA GCTAGACGTC TTCAAGCCAA AATGATGACT 
AATTTAGTTA TGGCCAAGGA CCGTTTACAA CTTCTAGAAA GTGGAGCAGT TCCGAAGAGG 
AAAGACCCCT TAACACATGC TAGTAATTCA TTGCCTCGAT CAAAAACTGT CCTGAAAAGT 
GGCTCCGCAG GGCTCTCCGG TCACCACAGG GCGCCTAGTT GCAGTGGTTT GTCCATGGTT 
TCTGGAGCAA GACCGGGACC TGGTCCTGCA GCTACCACAC ATAAGGGTAC TCCAAAACCA 
AATAGAACCA ACAAACCTTC TACTCCCACA ACTGCAGTTC GGAAAAAGAA AGACTTGAAA 
AATTTTAGGA ATGTGGACAG CAATCTTGCT AACCTTATAA TGAATGAAAT TGTTGACAAT 
GGGACAGCTG TTAAGTTTGA TGACATAGCC GGGCAGGAGC TGGCAAAGCA AGCGCTGCAG 
GAGATTGTCA TCCTTCCTTC TCTGCGGCCT GAGTTGTTCA CAGGGCTCAG AGCTCCTGCT 
AGAGGCTTGT TACTCTTCGG TCCGCCAGGA AACGGAAAAA CAATGCTGGC TAAAGCAGTA 
GCTGCAGAGT CTAATGCGAC CTTTTTCAAC ATAAGTGCTG CCAGTTTAAC TTCAAAATAT 
GTGGGAGAAG GAGAGAAATT GGTGAGAGCT CTCTTTGCTG TGGCTCGAGA ACTTCAACCA 
TCTATAATTT TTATAGATGA AGTTGACAGT CTTTTGTGTG AGAGACGGGA AGGGGAGCAC 
GACGCTAGCA GACGGCTAAA GACGGAATTT TTAATAGAAT TTGACGGGGT GCAATCTGCT 
GGAGATGACA GAGTACTTGT AATGGGTGCA ACTAACAGGC CCCAAGAGCT TGATGAAGCT 
GTTCTCAGGC GTTTCATTAA ACGGGTATAT GTGTCCTTAC CAAATGAGGA GACAAGACTC 
CTTCTGCTTA AAAACCTGTT GTGTAAACAA GGAAGTCCAC TGACCCAAAA AGAACTCGCA 
CAGCTTGCTA GAATGACCGA TGGATACTCT GGAAGTGATC TGACCGCTTT GGCCAAGGAT 
GCAGCCCTGG GTCCTATCCG AGAACTGAAG CCAGAGCAGG TGAAGAATAT GTCTGCCAGT 
GAGATGAGAA ATATTCGATT ATCTGACTTC ACAGAATCCT TAAAAAAGAT AAAACGCAGT 
GTGAGTCCTC AGACCTTAGA AGCATACATA CGCTGGAACA AGGATTTTGG AGACACCACT 
GTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Spastin regulates VAMP7-containing vesicles trafficking in cortical neurons.
Biochimica et biophysica acta1863(6)1666-1677(2017 Jun)
Plaud C,Joshi V,Marinello M,Pastr? D,Galli T,Curmi PA,Burgo A

Branch-Specific Microtubule Destabilization Mediates Axon Branch Loss during Neuromuscular Synapse Elimination.
Neuron92(4)845-856(2016 Nov)
Brill MS,Kleele T,Ruschkies L,Wang M,Marahori NA,Reuter MS,Hausrat TJ,Weigand E,Fisher M,Ahles A,Engelhardt S,Bishop DL,Kneussel M,Misgeld T

Amyloid-? oligomers induce synaptic damage via Tau-dependent microtubule severing by TTLL6 and spastin.
The EMBO journal32(22)2920-37(2013 Nov)
Zempel H,Luedtke J,Kumar Y,Biernat J,Dawson H,Mandelkow E,Mandelkow EM

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Spast cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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