Dnaaf5 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dnaaf5
Entrez Gene ID	433956
Full Name	dynein, axonemal assembly factor 5
Synonyms	BC053401,C76907,Heatr2
General protein information	Preferred Names dynein, axonemal assembly factor 5 Names dynein assembly factor 5, axonemal HEAT repeat containing 2 HEAT repeat-containing protein 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 G2

mRNA and Protein(s)

mRNA	Protein	Name
NM_001363485.1	NP_001350414.1	dynein assembly factor 5, axonemal isoform 2
NM_001081265.1	NP_001074734.1	dynein assembly factor 5, axonemal
NM_001081265.2	NP_001074734.1	dynein assembly factor 5, axonemal isoform 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	HEATR2	NP_060272.3
Bos taurus (cattle)	HEATR2	XP_003587898.1
Danio rerio (zebrafish)	LOC100333660	XP_002667597.3
Canis lupus familiaris (dog)	HEATR2	XP_548719.2
	HEATR2	XP_001097048.1
Mus musculus (house mouse)	Heatr2	NP_001074734.1
Gallus gallus (chicken)	HEATR2	XP_414755.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002100	XP_320940.5
Xenopus tropicalis (tropical clawed frog)	heatr2	XP_004918031.1
Rattus norvegicus (Norway rat)	Heatr2	NP_001128329.1
Drosophila melanogaster (fruit fly)	CG31320	NP_731905.1

Gene Ontology
Bibliography

Related articles in PubMed

HEATR2 plays a conserved role in assembly of the ciliary motile apparatus.
Diggle CP, Moore DJ, Mali G, zur Lage P, Ait-Lounis A, Schmidts M, Shoemark A, Garcia Munoz A, Halachev MR, Gautier P, Yeyati PL, Bonthron DT, Carr IM, Hayward B, Markham AF, Hope JE, von Kriegsheim A, Mitchison HM, Jackson IJ, Durand B, Reith W, Sheridan E, Jarman AP, Mill P
PLoS genetics10(9)e1004577(2014 Sep)

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE
Nucleic acids research31(1)278-81(2003 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dnaaf5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dnaaf5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu84255	NM_001363485.1 Latest version!	Mus musculus dynein, axonemal assembly factor 5 (Dnaaf5), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu01987	NM_001081265.2 Latest version!	Mus musculus dynein, axonemal assembly factor 5 (Dnaaf5), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$496.30 $709.00
View Old Accession Versions >>
OMu01987	NM_001081265.1	Mus musculus dynein, axonemal assembly factor 5 (Dnaaf5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$496.30 $709.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dnaaf5 ( NM_001363485.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001350414.1 Mus musculus dynein, axonemal assembly factor 5 (Dnaaf5), transcript variant 2, mRNA.

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CloneID	OMu84255
Clone ID Related Accession (Same CDS sequence)	NM_001363485.1
Accession Version	NM_001363485.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1677bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Mus musculus(house mouse)
Product	dynein assembly factor 5, axonemal isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC158066.1, BG228650.1 and CK627764.1. ##Evidence-Data-START## Transcript exon combination :: BC158066.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCTGAGA TCCGGCAGAC AGCCACCAGC CTATGGGAGA AGGTCGGGCT GCAGTGGCAG 
CAGGAGAACG AGGCAGATCT AAAGGACAAG CTGGACTTCG CTTCACCTCC TCCTCCCAAC 
TACCCTGAGC ACGAGAGCCG CCCTGGCCTG GGCTGCCGAG AGCTGGTCTT CAGGAACCTC 
TCCAAGGTGC TTCCTGCCAT CTGTCATGAT ATCACCGACT GGGTGGTGGG GACGCGGGTG 
AAGGCAGCAC AGCTGCTGCC GGTGCTGCTG CTGCACGCAG AGGACCACAT CACCCAGCAC 
CTGGAGATCG TCCTCAGAAC CCTGCATCAG GCCTGCACCG ATGAAGAGAA GGCTGTGGTC 
GGCAGTTGCA TAAGAGCCGC AGAGCTCATT GGCACCTTTG TCAGCCCAGA AGTATTTCTG 
AAACTGATCT TAGCCATGCT GAAGAAAGCA CCCTCTGCCT CTGGCCTCTT GATCCTCGCT 
TCTGTCATCC GGGGCTGCCC CCGGAATGCC CTCCAGCCAC ACGTCACAGT CATAGCCACA 
GAGCTGGCCC AGGAGCACAT CTGCCAGGGG TCTGAGAATA ATCTCTACCT GGAGCATCTG 
CTGCTGTGTG TGCAGGCCCT GCTGTCTGTA TGTCAGGAAG ACTGTCGAGC TGCCAGCCTG 
CAGTTCCTGG AGGTGCTGGT GACAATAATG GCAGTCTCAG ATGCCGTGGG TCTTGAGAAG 
AAGGCCCAGA AGACCATGGA CACACTGGCT GAGGTGGAGG ATATCCCCAG CAGTCAGGAC 
CTCTACCGCA AGCATGTGGG TGCACTCCTG GAGCGGCTGA CAGCCTCGCA TGGCGAGTGG 
GCAGTGCACT CTGTGCAGTT GCTGAAGTTC ACTGTGCTCC TCACCCAGGC AGGCCCGGCT 
GTGGGAGAAG CGCTGCAGCA TGTGATCCCC ACCCTCAGGG CCTGTCTCCA GCCCTCCACA 
GACCCACACA TGCGCCTGAA GCTCTTCTCT ATCCTGTCCA TGATGCTGCT GAGGCCTAAG 
GACACTGTCG ATTCTCAGGG ACAGTTCCGT GGCTATTTGG ATATGGTAAT AAATGACATC 
CTAGCCCCCA ACCTGCAGTG GCATGCAGGG AGGACAGCTG CAGCCATCCG GACGGCTGCT 
ATATCCTGCC TGTGGGCACT CATCAGCAGT GACATCTTAT CGGCCAAGCA GGTGCAAGAG 
GCACAAGAGA CCCTGATGCC ACAAGTGCTG GCTACGCTGG AGGACGACTC CCAGACCACA 
CGCCTGATGT CCTGCCGCAT CATCAACATG TTCCTCAAGA ATTCAGGGGA CACCATGGAG 
CCAGAGAAAT TCCTCAAGGT CTATCCTGAG CTCTTGAAGC GCCTAGATGA TGTCTCCAAT 
GATGTGAGGA TGGCAGCTGC TTCTGCCTTG CTCACCTGGC TCAAGTGCAT CGAGAGCTTG 
GATGGGAAGT CCGCCTATCA GAGCAGCGTT CAGTTCCTGT ACCGGGAGCT GCTGGTCCAC 
CTGGATGACC CAGAGAGTGC CATTCAAGAC ACCGTCCTAG AAGTCCTCAA GGAGGGCAGC 
GTCCTGTTCC CAGATGTGCT GGTGCGGGAG ACGGAGGCTG TCGTCCACAA ACACCGCTCA 
GCTACCTACT GCGAGCAGCT GCTGCAGCAC ATGCAGACCA TGGCCGCTGC TCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001350414.1
CDS	362..2038
Translation	MPEIRQTATSLWEKVGLQWQQENEADLKDKLDFASPPPPNYPEHESRPGLGCRELVFRNLSKVLPAICHDITDWVVGTRVKAAQLLPVLLLHAEDHITQHLEIVLRTLHQACTDEEKAVVGSCIRAAELIGTFVSPEVFLKLILAMLKKAPSASGLLILASVIRGCPRNALQPHVTVIATELAQEHICQGSENNLYLEHLLLCVQALLSVCQEDCRAASLQFLEVLVTIMAVSDAVGLEKKAQKTMDTLAEVEDIPSSQDLYRKHVGALLERLTASHGEWAVHSVQLLKFTVLLTQAGPAVGEALQHVIPTLRACLQPSTDPHMRLKLFSILSMMLLRPKDTVDSQGQFRGYLDMVINDILAPNLQWHAGRTAAAIRTAAISCLWALISSDILSAKQVQEAQETLMPQVLATLEDDSQTTRLMSCRIINMFLKNSGDTMEPEKFLKVYPELLKRLDDVSNDVRMAAASALLTWLKCIESLDGKSAYQSSVQFLYRELLVHLDDPESAIQDTVLEVLKEGSVLFPDVLVRETEAVVHKHRSATYCEQLLQHMQTMAAAR

Target ORF information:

RefSeq Version	NM_001363485.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus dynein, axonemal assembly factor 5 (Dnaaf5), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001363485.1

ORF Insert Sequence:

ATGCCTGAGA TCCGGCAGAC AGCCACCAGC CTATGGGAGA AGGTCGGGCT GCAGTGGCAG 
CAGGAGAACG AGGCAGATCT AAAGGACAAG CTGGACTTCG CTTCACCTCC TCCTCCCAAC 
TACCCTGAGC ACGAGAGCCG CCCTGGCCTG GGCTGCCGAG AGCTGGTCTT CAGGAACCTC 
TCCAAGGTGC TTCCTGCCAT CTGTCATGAT ATCACCGACT GGGTGGTGGG GACGCGGGTG 
AAGGCAGCAC AGCTGCTGCC GGTGCTGCTG CTGCACGCAG AGGACCACAT CACCCAGCAC 
CTGGAGATCG TCCTCAGAAC CCTGCATCAG GCCTGCACCG ATGAAGAGAA GGCTGTGGTC 
GGCAGTTGCA TAAGAGCCGC AGAGCTCATT GGCACCTTTG TCAGCCCAGA AGTATTTCTG 
AAACTGATCT TAGCCATGCT GAAGAAAGCA CCCTCTGCCT CTGGCCTCTT GATCCTCGCT 
TCTGTCATCC GGGGCTGCCC CCGGAATGCC CTCCAGCCAC ACGTCACAGT CATAGCCACA 
GAGCTGGCCC AGGAGCACAT CTGCCAGGGG TCTGAGAATA ATCTCTACCT GGAGCATCTG 
CTGCTGTGTG TGCAGGCCCT GCTGTCTGTA TGTCAGGAAG ACTGTCGAGC TGCCAGCCTG 
CAGTTCCTGG AGGTGCTGGT GACAATAATG GCAGTCTCAG ATGCCGTGGG TCTTGAGAAG 
AAGGCCCAGA AGACCATGGA CACACTGGCT GAGGTGGAGG ATATCCCCAG CAGTCAGGAC 
CTCTACCGCA AGCATGTGGG TGCACTCCTG GAGCGGCTGA CAGCCTCGCA TGGCGAGTGG 
GCAGTGCACT CTGTGCAGTT GCTGAAGTTC ACTGTGCTCC TCACCCAGGC AGGCCCGGCT 
GTGGGAGAAG CGCTGCAGCA TGTGATCCCC ACCCTCAGGG CCTGTCTCCA GCCCTCCACA 
GACCCACACA TGCGCCTGAA GCTCTTCTCT ATCCTGTCCA TGATGCTGCT GAGGCCTAAG 
GACACTGTCG ATTCTCAGGG ACAGTTCCGT GGCTATTTGG ATATGGTAAT AAATGACATC 
CTAGCCCCCA ACCTGCAGTG GCATGCAGGG AGGACAGCTG CAGCCATCCG GACGGCTGCT 
ATATCCTGCC TGTGGGCACT CATCAGCAGT GACATCTTAT CGGCCAAGCA GGTGCAAGAG 
GCACAAGAGA CCCTGATGCC ACAAGTGCTG GCTACGCTGG AGGACGACTC CCAGACCACA 
CGCCTGATGT CCTGCCGCAT CATCAACATG TTCCTCAAGA ATTCAGGGGA CACCATGGAG 
CCAGAGAAAT TCCTCAAGGT CTATCCTGAG CTCTTGAAGC GCCTAGATGA TGTCTCCAAT 
GATGTGAGGA TGGCAGCTGC TTCTGCCTTG CTCACCTGGC TCAAGTGCAT CGAGAGCTTG 
GATGGGAAGT CCGCCTATCA GAGCAGCGTT CAGTTCCTGT ACCGGGAGCT GCTGGTCCAC 
CTGGATGACC CAGAGAGTGC CATTCAAGAC ACCGTCCTAG AAGTCCTCAA GGAGGGCAGC 
GTCCTGTTCC CAGATGTGCT GGTGCGGGAG ACGGAGGCTG TCGTCCACAA ACACCGCTCA 
GCTACCTACT GCGAGCAGCT GCTGCAGCAC ATGCAGACCA TGGCCGCTGC TCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dnaaf5 ( NM_001081265.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001074734.1 Mus musculus dynein, axonemal assembly factor 5 (Dnaaf5), mRNA.

Price & Availability↑

CloneID	OMu01987
Clone ID Related Accession (Same CDS sequence)	NM_001081265.1 , NM_001081265.2
Accession Version	NM_001081265.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2562bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-19
Organism	Mus musculus(house mouse)
Product	dynein assembly factor 5, axonemal
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC125065.3. On or before Mar 24, 2007 this sequence version replaced NM_001004181.1, XM_620267.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC147492.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGCGC CGGCGGAGGC GGAGGTGGCC GCGGCCCCGG GCTTAACCGA GGCGGCTGAA 
GCCGCGGAAC TGACCCGCGC GCTAAGCCGC CTGCTACCGG GGCTGGAGAC GGAAAGTAAG 
CTGGGGCGGC GGCGCGCTCT GGAGGCTCTG GAGCAGGTAC TGGAAGAGGC GGTGCGGCCC 
GGCGCGGATT CCGCGGCCTT CCAGGGCCCC TGGGCCCGTC TGCTGCTGCC GCGCCTGCTG 
CGGCTTCTAA GCGATCCGGC CGAGGGCTGC CGCGCGCTGG CCGCTCACCT GCTCGACCTT 
GGCCTGCGCC GCGCCGCGCG GCCCCGCGAC GCTCTGCCGC GTCTGCTGCC GGCGCTCAGC 
GCACGCCTGG CCCGACCTGA ACTCGCGCGG CCGCCGCCGG AGCCCTGCGA GGAGCTGCGT 
CTCGCCCTCG TGCAGCTGCT CCACCTCGCC GTAGACCTGG GCGGCGCCGC GCTCGCGCCC 
CACCTGGACG ACGCCGTGCG CGCCTTGCGG GCCGCGCTGC TCGACCCCTT CGCTGCGGTG 
CGCCGGGAGG GCTGCGAGTG CGCAGCCGCT CTGGCCCGTG CCACTCCGGA GCACTTCCAC 
ATGCAATCGG AGTCCTTGAT AGGCCCCCTG ATGCAGACCA TCTCCCACCA GCACTGGAAG 
GTCCGGGTAG CCGTTATCGA AGCCACGGGC ACTGTGATCC AGTTTGGCAG TGGGAACTCG 
GTGGATGATG TCCTTTCCCA CTTTGCTCAA AGACTCTTCG ATGACGTCCC TCAGGTCCGG 
CAGGCAGTGA CCTCGGTGGT CGGGGGATGG CTGCTGAATC TCCGGGACCG CTACTCCTTC 
CTACACAAGC TCACCCCACT GCTGCTCAGC AGCTTCAGTG ATGAAATGCC TGAGATCCGG 
CAGACAGCCA CCAGCCTATG GGAGAAGGTC GGGCTGCAGT GGCAGCAGGA GAACGAGGCA 
GATCTAAAGG ACAAGCTGGA CTTCGCTTCA CCTCCTCCTC CCAACTACCC TGAGCACGAG 
AGCCGCCCTG GCCTGGGCTG CCGAGAGCTG GTCTTCAGGA ACCTCTCCAA GGTGCTTCCT 
GCCATCTGTC ATGATATCAC CGACTGGGTG GTGGGGACGC GGGTGAAGGC AGCACAGCTG 
CTGCCGGTGC TGCTGCTGCA CGCAGAGGAC CACATCACCC AGCACCTGGA GATCGTCCTC 
AGAACCCTGC ATCAGGCCTG CACCGATGAA GAGAAGGCTG TGGTCGGCAG TTGCATAAGA 
GCCGCAGAGC TCATTGGCAC CTTTGTCAGC CCAGAAGTAT TTCTGAAACT GATCTTAGCC 
ATGCTGAAGA AAGCACCCTC TGCCTCTGGC CTCTTGATCC TCGCTTCTGT CATCCGGGGC 
TGCCCCCGGA ATGCCCTCCA GCCACACGTC ACAGTCATAG CCACAGAGCT GGCCCAGGAG 
CACATCTGCC AGGGGTCTGA GAATAATCTC TACCTGGAGC ATCTGCTGCT GTGTGTGCAG 
GCCCTGCTGT CTGTATGTCA GGAAGACTGT CGAGCTGCCA GCCTGCAGTT CCTGGAGGTG 
CTGGTGACAA TAATGGCAGT CTCAGATGCC GTGGGTCTTG AGAAGAAGGC CCAGAAGACC 
ATGGACACAC TGGCTGAGGT GGAGGATATC CCCAGCAGTC AGGACCTCTA CCGCAAGCAT 
GTGGGTGCAC TCCTGGAGCG GCTGACAGCC TCGCATGGCG AGTGGGCAGT GCACTCTGTG 
CAGTTGCTGA AGTTCACTGT GCTCCTCACC CAGGCAGGCC CGGCTGTGGG AGAAGCGCTG 
CAGCATGTGA TCCCCACCCT CAGGGCCTGT CTCCAGCCCT CCACAGACCC ACACATGCGC 
CTGAAGCTCT TCTCTATCCT GTCCATGATG CTGCTGAGGC CTAAGGACAC TGTCGATTCT 
CAGGGACAGT TCCGTGGCTA TTTGGATATG GTAATAAATG ACATCCTAGC CCCCAACCTG 
CAGTGGCATG CAGGGAGGAC AGCTGCAGCC ATCCGGACGG CTGCTATATC CTGCCTGTGG 
GCACTCATCA GCAGTGACAT CTTATCGGCC AAGCAGGTGC AAGAGGCACA AGAGACCCTG 
ATGCCACAAG TGCTGGCTAC GCTGGAGGAC GACTCCCAGA CCACACGCCT GATGTCCTGC 
CGCATCATCA ACATGTTCCT CAAGAATTCA GGGGACACCA TGGAGCCAGA GAAATTCCTC 
AAGGTCTATC CTGAGCTCTT GAAGCGCCTA GATGATGTCT CCAATGATGT GAGGATGGCA 
GCTGCTTCTG CCTTGCTCAC CTGGCTCAAG TGCATCGAGA GCTTGGATGG GAAGTCCGCC 
TATCAGAGCA GCGTTCAGTT CCTGTACCGG GAGCTGCTGG TCCACCTGGA TGACCCAGAG 
AGTGCCATTC AAGACACCGT CCTAGAAGTC CTCAAGGAGG GCAGCGTCCT GTTCCCAGAT 
GTGCTGGTGC GGGAGACGGA GGCTGTCGTC CACAAACACC GCTCAGCTAC CTACTGCGAG 
CAGCTGCTGC AGCACATGCA GACCATGGCC GCTGCTCGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001074734.1
CDS	25..2586
Translation	MAAPAEAEVAAAPGLTEAAEAAELTRALSRLLPGLETESKLGRRRALEALEQVLEEAVRPGADSAAFQGPWARLLLPRLLRLLSDPAEGCRALAAHLLDLGLRRAARPRDALPRLLPALSARLARPELARPPPEPCEELRLALVQLLHLAVDLGGAALAPHLDDAVRALRAALLDPFAAVRREGCECAAALARATPEHFHMQSESLIGPLMQTISHQHWKVRVAVIEATGTVIQFGSGNSVDDVLSHFAQRLFDDVPQVRQAVTSVVGGWLLNLRDRYSFLHKLTPLLLSSFSDEMPEIRQTATSLWEKVGLQWQQENEADLKDKLDFASPPPPNYPEHESRPGLGCRELVFRNLSKVLPAICHDITDWVVGTRVKAAQLLPVLLLHAEDHITQHLEIVLRTLHQACTDEEKAVVGSCIRAAELIGTFVSPEVFLKLILAMLKKAPSASGLLILASVIRGCPRNALQPHVTVIATELAQEHICQGSENNLYLEHLLLCVQALLSVCQEDCRAASLQFLEVLVTIMAVSDAVGLEKKAQKTMDTLAEVEDIPSSQDLYRKHVGALLERLTASHGEWAVHSVQLLKFTVLLTQAGPAVGEALQHVIPTLRACLQPSTDPHMRLKLFSILSMMLLRPKDTVDSQGQFRGYLDMVINDILAPNLQWHAGRTAAAIRTAAISCLWALISSDILSAKQVQEAQETLMPQVLATLEDDSQTTRLMSCRIINMFLKNSGDTMEPEKFLKVYPELLKRLDDVSNDVRMAAASALLTWLKCIESLDGKSAYQSSVQFLYRELLVHLDDPESAIQDTVLEVLKEGSVLFPDVLVRETEAVVHKHRSATYCEQLLQHMQTMAAAR

Target ORF information:

RefSeq Version	NM_001081265.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus dynein, axonemal assembly factor 5 (Dnaaf5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001081265.1

ORF Insert Sequence:

ATGGCGGCGC CGGCGGAGGC GGAGGTGGCC GCGGCCCCGG GCTTAACCGA GGCGGCTGAA 
GCCGCGGAAC TGACCCGCGC GCTAAGCCGC CTGCTACCGG GGCTGGAGAC GGAAAGTAAG 
CTGGGGCGGC GGCGCGCTCT GGAGGCTCTG GAGCAGGTAC TGGAAGAGGC GGTGCGGCCC 
GGCGCGGATT CCGCGGCCTT CCAGGGCCCC TGGGCCCGTC TGCTGCTGCC GCGCCTGCTG 
CGGCTTCTAA GCGATCCGGC CGAGGGCTGC CGCGCGCTGG CCGCTCACCT GCTCGACCTT 
GGCCTGCGCC GCGCCGCGCG GCCCCGCGAC GCTCTGCCGC GTCTGCTGCC GGCGCTCAGC 
GCACGCCTGG CCCGACCTGA ACTCGCGCGG CCGCCGCCGG AGCCCTGCGA GGAGCTGCGT 
CTCGCCCTCG TGCAGCTGCT CCACCTCGCC GTAGACCTGG GCGGCGCCGC GCTCGCGCCC 
CACCTGGACG ACGCCGTGCG CGCCTTGCGG GCCGCGCTGC TCGACCCCTT CGCTGCGGTG 
CGCCGGGAGG GCTGCGAGTG CGCAGCCGCT CTGGCCCGTG CCACTCCGGA GCACTTCCAC 
ATGCAATCGG AGTCCTTGAT AGGCCCCCTG ATGCAGACCA TCTCCCACCA GCACTGGAAG 
GTCCGGGTAG CCGTTATCGA AGCCACGGGC ACTGTGATCC AGTTTGGCAG TGGGAACTCG 
GTGGATGATG TCCTTTCCCA CTTTGCTCAA AGACTCTTCG ATGACGTCCC TCAGGTCCGG 
CAGGCAGTGA CCTCGGTGGT CGGGGGATGG CTGCTGAATC TCCGGGACCG CTACTCCTTC 
CTACACAAGC TCACCCCACT GCTGCTCAGC AGCTTCAGTG ATGAAATGCC TGAGATCCGG 
CAGACAGCCA CCAGCCTATG GGAGAAGGTC GGGCTGCAGT GGCAGCAGGA GAACGAGGCA 
GATCTAAAGG ACAAGCTGGA CTTCGCTTCA CCTCCTCCTC CCAACTACCC TGAGCACGAG 
AGCCGCCCTG GCCTGGGCTG CCGAGAGCTG GTCTTCAGGA ACCTCTCCAA GGTGCTTCCT 
GCCATCTGTC ATGATATCAC CGACTGGGTG GTGGGGACGC GGGTGAAGGC AGCACAGCTG 
CTGCCGGTGC TGCTGCTGCA CGCAGAGGAC CACATCACCC AGCACCTGGA GATCGTCCTC 
AGAACCCTGC ATCAGGCCTG CACCGATGAA GAGAAGGCTG TGGTCGGCAG TTGCATAAGA 
GCCGCAGAGC TCATTGGCAC CTTTGTCAGC CCAGAAGTAT TTCTGAAACT GATCTTAGCC 
ATGCTGAAGA AAGCACCCTC TGCCTCTGGC CTCTTGATCC TCGCTTCTGT CATCCGGGGC 
TGCCCCCGGA ATGCCCTCCA GCCACACGTC ACAGTCATAG CCACAGAGCT GGCCCAGGAG 
CACATCTGCC AGGGGTCTGA GAATAATCTC TACCTGGAGC ATCTGCTGCT GTGTGTGCAG 
GCCCTGCTGT CTGTATGTCA GGAAGACTGT CGAGCTGCCA GCCTGCAGTT CCTGGAGGTG 
CTGGTGACAA TAATGGCAGT CTCAGATGCC GTGGGTCTTG AGAAGAAGGC CCAGAAGACC 
ATGGACACAC TGGCTGAGGT GGAGGATATC CCCAGCAGTC AGGACCTCTA CCGCAAGCAT 
GTGGGTGCAC TCCTGGAGCG GCTGACAGCC TCGCATGGCG AGTGGGCAGT GCACTCTGTG 
CAGTTGCTGA AGTTCACTGT GCTCCTCACC CAGGCAGGCC CGGCTGTGGG AGAAGCGCTG 
CAGCATGTGA TCCCCACCCT CAGGGCCTGT CTCCAGCCCT CCACAGACCC ACACATGCGC 
CTGAAGCTCT TCTCTATCCT GTCCATGATG CTGCTGAGGC CTAAGGACAC TGTCGATTCT 
CAGGGACAGT TCCGTGGCTA TTTGGATATG GTAATAAATG ACATCCTAGC CCCCAACCTG 
CAGTGGCATG CAGGGAGGAC AGCTGCAGCC ATCCGGACGG CTGCTATATC CTGCCTGTGG 
GCACTCATCA GCAGTGACAT CTTATCGGCC AAGCAGGTGC AAGAGGCACA AGAGACCCTG 
ATGCCACAAG TGCTGGCTAC GCTGGAGGAC GACTCCCAGA CCACACGCCT GATGTCCTGC 
CGCATCATCA ACATGTTCCT CAAGAATTCA GGGGACACCA TGGAGCCAGA GAAATTCCTC 
AAGGTCTATC CTGAGCTCTT GAAGCGCCTA GATGATGTCT CCAATGATGT GAGGATGGCA 
GCTGCTTCTG CCTTGCTCAC CTGGCTCAAG TGCATCGAGA GCTTGGATGG GAAGTCCGCC 
TATCAGAGCA GCGTTCAGTT CCTGTACCGG GAGCTGCTGG TCCACCTGGA TGACCCAGAG 
AGTGCCATTC AAGACACCGT CCTAGAAGTC CTCAAGGAGG GCAGCGTCCT GTTCCCAGAT 
GTGCTGGTGC GGGAGACGGA GGCTGTCGTC CACAAACACC GCTCAGCTAC CTACTGCGAG 
CAGCTGCTGC AGCACATGCA GACCATGGCC GCTGCTCGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dnaaf5 ( NM_001081265.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001074734.1 Mus musculus dynein, axonemal assembly factor 5 (Dnaaf5), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu01987
Clone ID Related Accession (Same CDS sequence)	NM_001081265.1 , NM_001081265.2
Accession Version	NM_001081265.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2562bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Mus musculus(house mouse)
Product	dynein assembly factor 5, axonemal isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC125065.3. On May 19, 2018 this sequence version replaced NM_001081265.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC147492.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGC CGGCGGAGGC GGAGGTGGCC GCGGCCCCGG GCTTAACCGA GGCGGCTGAA 
GCCGCGGAAC TGACCCGCGC GCTAAGCCGC CTGCTACCGG GGCTGGAGAC GGAAAGTAAG 
CTGGGGCGGC GGCGCGCTCT GGAGGCTCTG GAGCAGGTAC TGGAAGAGGC GGTGCGGCCC 
GGCGCGGATT CCGCGGCCTT CCAGGGCCCC TGGGCCCGTC TGCTGCTGCC GCGCCTGCTG 
CGGCTTCTAA GCGATCCGGC CGAGGGCTGC CGCGCGCTGG CCGCTCACCT GCTCGACCTT 
GGCCTGCGCC GCGCCGCGCG GCCCCGCGAC GCTCTGCCGC GTCTGCTGCC GGCGCTCAGC 
GCACGCCTGG CCCGACCTGA ACTCGCGCGG CCGCCGCCGG AGCCCTGCGA GGAGCTGCGT 
CTCGCCCTCG TGCAGCTGCT CCACCTCGCC GTAGACCTGG GCGGCGCCGC GCTCGCGCCC 
CACCTGGACG ACGCCGTGCG CGCCTTGCGG GCCGCGCTGC TCGACCCCTT CGCTGCGGTG 
CGCCGGGAGG GCTGCGAGTG CGCAGCCGCT CTGGCCCGTG CCACTCCGGA GCACTTCCAC 
ATGCAATCGG AGTCCTTGAT AGGCCCCCTG ATGCAGACCA TCTCCCACCA GCACTGGAAG 
GTCCGGGTAG CCGTTATCGA AGCCACGGGC ACTGTGATCC AGTTTGGCAG TGGGAACTCG 
GTGGATGATG TCCTTTCCCA CTTTGCTCAA AGACTCTTCG ATGACGTCCC TCAGGTCCGG 
CAGGCAGTGA CCTCGGTGGT CGGGGGATGG CTGCTGAATC TCCGGGACCG CTACTCCTTC 
CTACACAAGC TCACCCCACT GCTGCTCAGC AGCTTCAGTG ATGAAATGCC TGAGATCCGG 
CAGACAGCCA CCAGCCTATG GGAGAAGGTC GGGCTGCAGT GGCAGCAGGA GAACGAGGCA 
GATCTAAAGG ACAAGCTGGA CTTCGCTTCA CCTCCTCCTC CCAACTACCC TGAGCACGAG 
AGCCGCCCTG GCCTGGGCTG CCGAGAGCTG GTCTTCAGGA ACCTCTCCAA GGTGCTTCCT 
GCCATCTGTC ATGATATCAC CGACTGGGTG GTGGGGACGC GGGTGAAGGC AGCACAGCTG 
CTGCCGGTGC TGCTGCTGCA CGCAGAGGAC CACATCACCC AGCACCTGGA GATCGTCCTC 
AGAACCCTGC ATCAGGCCTG CACCGATGAA GAGAAGGCTG TGGTCGGCAG TTGCATAAGA 
GCCGCAGAGC TCATTGGCAC CTTTGTCAGC CCAGAAGTAT TTCTGAAACT GATCTTAGCC 
ATGCTGAAGA AAGCACCCTC TGCCTCTGGC CTCTTGATCC TCGCTTCTGT CATCCGGGGC 
TGCCCCCGGA ATGCCCTCCA GCCACACGTC ACAGTCATAG CCACAGAGCT GGCCCAGGAG 
CACATCTGCC AGGGGTCTGA GAATAATCTC TACCTGGAGC ATCTGCTGCT GTGTGTGCAG 
GCCCTGCTGT CTGTATGTCA GGAAGACTGT CGAGCTGCCA GCCTGCAGTT CCTGGAGGTG 
CTGGTGACAA TAATGGCAGT CTCAGATGCC GTGGGTCTTG AGAAGAAGGC CCAGAAGACC 
ATGGACACAC TGGCTGAGGT GGAGGATATC CCCAGCAGTC AGGACCTCTA CCGCAAGCAT 
GTGGGTGCAC TCCTGGAGCG GCTGACAGCC TCGCATGGCG AGTGGGCAGT GCACTCTGTG 
CAGTTGCTGA AGTTCACTGT GCTCCTCACC CAGGCAGGCC CGGCTGTGGG AGAAGCGCTG 
CAGCATGTGA TCCCCACCCT CAGGGCCTGT CTCCAGCCCT CCACAGACCC ACACATGCGC 
CTGAAGCTCT TCTCTATCCT GTCCATGATG CTGCTGAGGC CTAAGGACAC TGTCGATTCT 
CAGGGACAGT TCCGTGGCTA TTTGGATATG GTAATAAATG ACATCCTAGC CCCCAACCTG 
CAGTGGCATG CAGGGAGGAC AGCTGCAGCC ATCCGGACGG CTGCTATATC CTGCCTGTGG 
GCACTCATCA GCAGTGACAT CTTATCGGCC AAGCAGGTGC AAGAGGCACA AGAGACCCTG 
ATGCCACAAG TGCTGGCTAC GCTGGAGGAC GACTCCCAGA CCACACGCCT GATGTCCTGC 
CGCATCATCA ACATGTTCCT CAAGAATTCA GGGGACACCA TGGAGCCAGA GAAATTCCTC 
AAGGTCTATC CTGAGCTCTT GAAGCGCCTA GATGATGTCT CCAATGATGT GAGGATGGCA 
GCTGCTTCTG CCTTGCTCAC CTGGCTCAAG TGCATCGAGA GCTTGGATGG GAAGTCCGCC 
TATCAGAGCA GCGTTCAGTT CCTGTACCGG GAGCTGCTGG TCCACCTGGA TGACCCAGAG 
AGTGCCATTC AAGACACCGT CCTAGAAGTC CTCAAGGAGG GCAGCGTCCT GTTCCCAGAT 
GTGCTGGTGC GGGAGACGGA GGCTGTCGTC CACAAACACC GCTCAGCTAC CTACTGCGAG 
CAGCTGCTGC AGCACATGCA GACCATGGCC GCTGCTCGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001074734.1
CDS	13..2574
Translation	MAAPAEAEVAAAPGLTEAAEAAELTRALSRLLPGLETESKLGRRRALEALEQVLEEAVRPGADSAAFQGPWARLLLPRLLRLLSDPAEGCRALAAHLLDLGLRRAARPRDALPRLLPALSARLARPELARPPPEPCEELRLALVQLLHLAVDLGGAALAPHLDDAVRALRAALLDPFAAVRREGCECAAALARATPEHFHMQSESLIGPLMQTISHQHWKVRVAVIEATGTVIQFGSGNSVDDVLSHFAQRLFDDVPQVRQAVTSVVGGWLLNLRDRYSFLHKLTPLLLSSFSDEMPEIRQTATSLWEKVGLQWQQENEADLKDKLDFASPPPPNYPEHESRPGLGCRELVFRNLSKVLPAICHDITDWVVGTRVKAAQLLPVLLLHAEDHITQHLEIVLRTLHQACTDEEKAVVGSCIRAAELIGTFVSPEVFLKLILAMLKKAPSASGLLILASVIRGCPRNALQPHVTVIATELAQEHICQGSENNLYLEHLLLCVQALLSVCQEDCRAASLQFLEVLVTIMAVSDAVGLEKKAQKTMDTLAEVEDIPSSQDLYRKHVGALLERLTASHGEWAVHSVQLLKFTVLLTQAGPAVGEALQHVIPTLRACLQPSTDPHMRLKLFSILSMMLLRPKDTVDSQGQFRGYLDMVINDILAPNLQWHAGRTAAAIRTAAISCLWALISSDILSAKQVQEAQETLMPQVLATLEDDSQTTRLMSCRIINMFLKNSGDTMEPEKFLKVYPELLKRLDDVSNDVRMAAASALLTWLKCIESLDGKSAYQSSVQFLYRELLVHLDDPESAIQDTVLEVLKEGSVLFPDVLVRETEAVVHKHRSATYCEQLLQHMQTMAAAR

Target ORF information:

RefSeq Version	NM_001081265.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus dynein, axonemal assembly factor 5 (Dnaaf5), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001081265.2

ORF Insert Sequence:

ATGGCGGCGC CGGCGGAGGC GGAGGTGGCC GCGGCCCCGG GCTTAACCGA GGCGGCTGAA 
GCCGCGGAAC TGACCCGCGC GCTAAGCCGC CTGCTACCGG GGCTGGAGAC GGAAAGTAAG 
CTGGGGCGGC GGCGCGCTCT GGAGGCTCTG GAGCAGGTAC TGGAAGAGGC GGTGCGGCCC 
GGCGCGGATT CCGCGGCCTT CCAGGGCCCC TGGGCCCGTC TGCTGCTGCC GCGCCTGCTG 
CGGCTTCTAA GCGATCCGGC CGAGGGCTGC CGCGCGCTGG CCGCTCACCT GCTCGACCTT 
GGCCTGCGCC GCGCCGCGCG GCCCCGCGAC GCTCTGCCGC GTCTGCTGCC GGCGCTCAGC 
GCACGCCTGG CCCGACCTGA ACTCGCGCGG CCGCCGCCGG AGCCCTGCGA GGAGCTGCGT 
CTCGCCCTCG TGCAGCTGCT CCACCTCGCC GTAGACCTGG GCGGCGCCGC GCTCGCGCCC 
CACCTGGACG ACGCCGTGCG CGCCTTGCGG GCCGCGCTGC TCGACCCCTT CGCTGCGGTG 
CGCCGGGAGG GCTGCGAGTG CGCAGCCGCT CTGGCCCGTG CCACTCCGGA GCACTTCCAC 
ATGCAATCGG AGTCCTTGAT AGGCCCCCTG ATGCAGACCA TCTCCCACCA GCACTGGAAG 
GTCCGGGTAG CCGTTATCGA AGCCACGGGC ACTGTGATCC AGTTTGGCAG TGGGAACTCG 
GTGGATGATG TCCTTTCCCA CTTTGCTCAA AGACTCTTCG ATGACGTCCC TCAGGTCCGG 
CAGGCAGTGA CCTCGGTGGT CGGGGGATGG CTGCTGAATC TCCGGGACCG CTACTCCTTC 
CTACACAAGC TCACCCCACT GCTGCTCAGC AGCTTCAGTG ATGAAATGCC TGAGATCCGG 
CAGACAGCCA CCAGCCTATG GGAGAAGGTC GGGCTGCAGT GGCAGCAGGA GAACGAGGCA 
GATCTAAAGG ACAAGCTGGA CTTCGCTTCA CCTCCTCCTC CCAACTACCC TGAGCACGAG 
AGCCGCCCTG GCCTGGGCTG CCGAGAGCTG GTCTTCAGGA ACCTCTCCAA GGTGCTTCCT 
GCCATCTGTC ATGATATCAC CGACTGGGTG GTGGGGACGC GGGTGAAGGC AGCACAGCTG 
CTGCCGGTGC TGCTGCTGCA CGCAGAGGAC CACATCACCC AGCACCTGGA GATCGTCCTC 
AGAACCCTGC ATCAGGCCTG CACCGATGAA GAGAAGGCTG TGGTCGGCAG TTGCATAAGA 
GCCGCAGAGC TCATTGGCAC CTTTGTCAGC CCAGAAGTAT TTCTGAAACT GATCTTAGCC 
ATGCTGAAGA AAGCACCCTC TGCCTCTGGC CTCTTGATCC TCGCTTCTGT CATCCGGGGC 
TGCCCCCGGA ATGCCCTCCA GCCACACGTC ACAGTCATAG CCACAGAGCT GGCCCAGGAG 
CACATCTGCC AGGGGTCTGA GAATAATCTC TACCTGGAGC ATCTGCTGCT GTGTGTGCAG 
GCCCTGCTGT CTGTATGTCA GGAAGACTGT CGAGCTGCCA GCCTGCAGTT CCTGGAGGTG 
CTGGTGACAA TAATGGCAGT CTCAGATGCC GTGGGTCTTG AGAAGAAGGC CCAGAAGACC 
ATGGACACAC TGGCTGAGGT GGAGGATATC CCCAGCAGTC AGGACCTCTA CCGCAAGCAT 
GTGGGTGCAC TCCTGGAGCG GCTGACAGCC TCGCATGGCG AGTGGGCAGT GCACTCTGTG 
CAGTTGCTGA AGTTCACTGT GCTCCTCACC CAGGCAGGCC CGGCTGTGGG AGAAGCGCTG 
CAGCATGTGA TCCCCACCCT CAGGGCCTGT CTCCAGCCCT CCACAGACCC ACACATGCGC 
CTGAAGCTCT TCTCTATCCT GTCCATGATG CTGCTGAGGC CTAAGGACAC TGTCGATTCT 
CAGGGACAGT TCCGTGGCTA TTTGGATATG GTAATAAATG ACATCCTAGC CCCCAACCTG 
CAGTGGCATG CAGGGAGGAC AGCTGCAGCC ATCCGGACGG CTGCTATATC CTGCCTGTGG 
GCACTCATCA GCAGTGACAT CTTATCGGCC AAGCAGGTGC AAGAGGCACA AGAGACCCTG 
ATGCCACAAG TGCTGGCTAC GCTGGAGGAC GACTCCCAGA CCACACGCCT GATGTCCTGC 
CGCATCATCA ACATGTTCCT CAAGAATTCA GGGGACACCA TGGAGCCAGA GAAATTCCTC 
AAGGTCTATC CTGAGCTCTT GAAGCGCCTA GATGATGTCT CCAATGATGT GAGGATGGCA 
GCTGCTTCTG CCTTGCTCAC CTGGCTCAAG TGCATCGAGA GCTTGGATGG GAAGTCCGCC 
TATCAGAGCA GCGTTCAGTT CCTGTACCGG GAGCTGCTGG TCCACCTGGA TGACCCAGAG 
AGTGCCATTC AAGACACCGT CCTAGAAGTC CTCAAGGAGG GCAGCGTCCT GTTCCCAGAT 
GTGCTGGTGC GGGAGACGGA GGCTGTCGTC CACAAACACC GCTCAGCTAC CTACTGCGAG 
CAGCTGCTGC AGCACATGCA GACCATGGCC GCTGCTCGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

HEATR2 plays a conserved role in assembly of the ciliary motile apparatus.
PLoS genetics10(9)e1004577(2014 Sep)
Diggle CP,Moore DJ,Mali G,zur Lage P,Ait-Lounis A,Schmidts M,Shoemark A,Garcia Munoz A,Halachev MR,Gautier P,Yeyati PL,Bonthron DT,Carr IM,Hayward B,Markham AF,Hope JE,von Kriegsheim A,Mitchison HM,Jackson IJ,Durand B,Reith W,Sheridan E,Jarman AP,Mill P

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Nucleic acids research31(1)278-81(2003 Jan)
Stryke D,Kawamoto M,Huang CC,Johns SJ,King LA,Harper CA,Meng EC,Lee RE,Yee A,L'Italien L,Chuang PT,Young SG,Skarnes WC,Babbitt PC,Ferrin TE

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Dnaaf5 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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