Dnaaf5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dnaaf5
Entrez Gene ID	304332
Full Name	dynein, axonemal, assembly factor 5
Synonyms	Heatr2
General protein information	Preferred Names dynein, axonemal, assembly factor 5 Names dynein assembly factor 5, axonemal HEAT repeat containing 2 HEAT repeat-containing protein 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 12 Map Location: 12q11

mRNA and Protein(s)

mRNA	Protein	Name
NM_001134857.1	NP_001128329.1	dynein assembly factor 5, axonemal

Pathways from BioSystems

Homologies

Homo sapiens (human)	HEATR2	NP_060272.3
Bos taurus (cattle)	HEATR2	XP_003587898.1
Danio rerio (zebrafish)	LOC100333660	XP_002667597.3
Canis lupus familiaris (dog)	HEATR2	XP_548719.2
	HEATR2	XP_001097048.1
Mus musculus (house mouse)	Heatr2	NP_001074734.1
Gallus gallus (chicken)	HEATR2	XP_414755.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002100	XP_320940.5
Xenopus tropicalis (tropical clawed frog)	heatr2	XP_004918031.1
Rattus norvegicus (Norway rat)	Heatr2	NP_001128329.1
Drosophila melanogaster (fruit fly)	CG31320	NP_731905.1

Gene Ontology
Bibliography

Related articles in PubMed

HEATR2 plays a conserved role in assembly of the ciliary motile apparatus.
Diggle CP, Moore DJ, Mali G, zur Lage P, Ait-Lounis A, Schmidts M, Shoemark A, Garcia Munoz A, Halachev MR, Gautier P, Yeyati PL, Bonthron DT, Carr IM, Hayward B, Markham AF, Hope JE, von Kriegsheim A, Mitchison HM, Jackson IJ, Durand B, Reith W, Sheridan E, Jarman AP, Mill P
PLoS genetics10(9)e1004577(2014 Sep)

Whole-exome capture and sequencing identifies HEATR2 mutation as a cause of primary ciliary dyskinesia.
Horani A, Druley TE, Zariwala MA, Patel AC, Levinson BT, Van Arendonk LG, Thornton KC, Giacalone JC, Albee AJ, Wilson KS, Turner EH, Nickerson DA, Shendure J, Bayly PV, Leigh MW, Knowles MR, Brody SL, Dutcher SK, Ferkol TW
American journal of human genetics91(4)685-93(2012 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dnaaf5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dnaaf5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa03679	NM_001134857.1 Latest version!	Rattus norvegicus dynein, axonemal, assembly factor 5 (Dnaaf5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dnaaf5 ( NM_001134857.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001128329.1 Rattus norvegicus dynein, axonemal, assembly factor 5 (Dnaaf5), mRNA.

Price & Availability↑

CloneID	ORa03679
Clone ID Related Accession (Same CDS sequence)	NM_001134857.1
Accession Version	NM_001134857.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2556bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-31
Organism	Rattus norvegicus(Norway rat)
Product	dynein assembly factor 5, axonemal
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC121192.5 and AC127903.3. On Sep 10, 2008 this sequence version replaced XM_001055205.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGCGC CGGCGGAGGC GGAGGTGGCC GCGGGCTTCG CCGAGGCGGA TGAAGCAGCG 
GAACTGACCC GCGCGCTAGG TCGTCTGTTA CCGGGACTGG AGACCGAAAG TAAGCTGGGG 
CGGCGGCGCG CGCTGGAAGC TCTGGAGCAC GCACTGGAAG AGGCGGTGCG GCCCGGAACG 
GATTCCGCTG CCTTCCAGGG CCCATGGGCT CGTCTGCTGC TGCCTCGCCT GCTGCGACTT 
CTGACCGACC CAGCCGAGGG CTGCCGCGCG CTGGCTGCTC ACCTGCTGGA CTTAGGCCTT 
CGCCGTGCCG CGCGGCCCCG CGATGCTCTG CCGCGCCTGT TGCCCGCGCT CACCGCACGC 
CTTGCCCGAC CAGAATCCAC GCGGCCACCG CCTGAACCCT GCGAAGAGCT GCGTCTTGCC 
CTCGTGCAAC TGCTTCACCT GGCCGTGGAC CTGGGCGGCG CCGCGCTCGC GCCCCACCTG 
GACGACGCGG TGCGCGCGTT GCGGGGTGCG CTGCTCGACC CCTTCGCCGC GGTGCGCCGG 
GAGGGTTGCG AGTGCGCAGC CGCTCTGGCG CGTGCCATTC CGGAGCACTT CCACATGCAG 
TCGGAGTCCC TCATAGGCCC CCTGATGCAG ACCATCTCCC ACCAGCACTG GAAGGTCCGG 
GTAGCTGTCA TCGAAGCCAC AGGCACTGTA ATCCAGTTTG GCAATGGGAA TTCGGTGGAT 
GATGTTCTTT CCCACTTTGC TCAAAGACTC TTCGATGATG TCCCTCAGGT CCGGCAGGCA 
GTGACCTCGG TGGTGGGGGG ATGGCTGCTC AATCTCCGTG ACCGATACTC CTTCTTTCAC 
AAACTCACCC CACTGTTACT CAGCAGCTTC AGTGACGAAA TGCCTGAGGT CCGACAGACA 
GCCACCAGCC TCTGGGAGAA GGTCGGGCTG CAGTGGCAGG AGGAAAATGA GGCAGATCTA 
AAGGACAAGC TGGACTTTGC TTCACCTCCT CCTCCTAACT ACCCTGAGCA CGAGAGCCGT 
CCTGGCCTGG GCTGCCGAGA GCTGGTCTTC AGGAACCTCT CCAAGGTGCT TCCTGCCATC 
TGTCACGATA TCACCGACTG GGTGGTGGGG ACCCGGCTGA AGGCAGCGCA GCTGCTGCCG 
GTGCTGCTGC TGCATGCAGA GGACCACATC ACACAGCACC TGGAGATCGT CCTCAGAACC 
CTGCATCAGG CGTGCACCGA TGAGGAGAAG GCTGTGGTCA GCAGCTGCGT GAGAGCCGCA 
GAGCTCATTG GCACCTTCGT CAGCCCAGAA GTATTTCTGA AACTGATCTT AGCCATGCTG 
AAGAAAGCAC CCTCGGCCTC TGGCCTCTTG ATCCTTGCTT CTGTCATCCG AGGCTGTCCC 
CGCAATGCCC TTCAGCCACA CCTCAAGGTC ATAGCCACAG AGCTGGCCCA GGAGCACATC 
TGCCAGGGGT CTGAGAATAA TAGCTACCTG GAGCATCTGC TGCTGTGTGT GCAGACTCTG 
CTGTCTGTGT GTCAGGAAGA CTGCTGTGCT GCCAGCCTGC AGCTCTCGGA GGTGCTGGTG 
ACAATAATGG CAGTCTCAGA TGCCGTGAGT CTTGAGAAGA AGGCTCAGAA GACCATGGAC 
ACCCTGGCTG AGGTGGAGGA TATCCCCAGC AGTCAGGACC TCTACCGCAA GCATCTGGGT 
GCACTACTGG AACGGCTGAC AGCCTCTCAT GGCGAGTGGA CGGTGCACTC TGTGCAGCTG 
CTGAAGTTCA CTGTGCTCCT CACCCAGGCA GGCCCAGCTG TGGGAGAGGC TCTGCAGCAT 
GTGATCCCCA CTCTCAGGGC CTGTCTCCAG CCCACCACAG ACCCACACAT GCGCCTGAAG 
CTCTTCTCTA TCCTGTCTTC ATTGCTGCTG AGGCCTAAGG ACACCGTCGA CTCTCAGGGA 
CAGTTCCATG GCTACTTGGA TATGGTAATA AATGACATCC TAGCCCCCAA CCTACAGTGG 
CATGCAGGCA GGACAGCTGC AGCCATCCGG ACGGCCACCA TATCCTGTCT GTGGGCACTT 
ATCAGCAGTG ACATCTTATC GGCCAAGCAG GTTCAAGAGG CACAGGAGAC CCTGATGCCA 
CAAGTGCTGG CCACGCTGGA GGACGACTCC CAGACCACAC GCCTGATCTC CTGCCGTATC 
ATCAGCAGGT TCCTCAAGAA CTCAGGGGAT ACCATGGAGC CAGAGAAATT TCTCAAGGTC 
TATCCTGAGC TCTTGAAGCG CCTAGATGAT GTCTCCAATG ACGTGAGAAT GGCAGCTGCT 
TCCGCCTTGC TCACCTGGCT GAAGTGCATT GAGAGCGTGG ATGGGAAATC CGCCTATCAG 
AGCAGCGTTC AGTTCCTGTA CAGGGAGCTG CTGGTCCACC TGGATGACCC AGAAAGTGCC 
ATCCAAGACA CTGTGCTAGA AGTCCTCAAG GAGGGCAGCG TCCTGTTTCC AGATGTGCTG 
GTGCGGGAGA CGGAGGCTGT TGTCCACAAA CACCGCTCAG CTACCTACTG CGAGCAGCTG 
CTGCAGCATA TGCAAACCAT GGCTGCCGCT CGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001128329.1
CDS	1..2556
Translation	MAAPAEAEVAAGFAEADEAAELTRALGRLLPGLETESKLGRRRALEALEHALEEAVRPGTDSAAFQGPWARLLLPRLLRLLTDPAEGCRALAAHLLDLGLRRAARPRDALPRLLPALTARLARPESTRPPPEPCEELRLALVQLLHLAVDLGGAALAPHLDDAVRALRGALLDPFAAVRREGCECAAALARAIPEHFHMQSESLIGPLMQTISHQHWKVRVAVIEATGTVIQFGNGNSVDDVLSHFAQRLFDDVPQVRQAVTSVVGGWLLNLRDRYSFFHKLTPLLLSSFSDEMPEVRQTATSLWEKVGLQWQEENEADLKDKLDFASPPPPNYPEHESRPGLGCRELVFRNLSKVLPAICHDITDWVVGTRLKAAQLLPVLLLHAEDHITQHLEIVLRTLHQACTDEEKAVVSSCVRAAELIGTFVSPEVFLKLILAMLKKAPSASGLLILASVIRGCPRNALQPHLKVIATELAQEHICQGSENNSYLEHLLLCVQTLLSVCQEDCCAASLQLSEVLVTIMAVSDAVSLEKKAQKTMDTLAEVEDIPSSQDLYRKHLGALLERLTASHGEWTVHSVQLLKFTVLLTQAGPAVGEALQHVIPTLRACLQPTTDPHMRLKLFSILSSLLLRPKDTVDSQGQFHGYLDMVINDILAPNLQWHAGRTAAAIRTATISCLWALISSDILSAKQVQEAQETLMPQVLATLEDDSQTTRLISCRIISRFLKNSGDTMEPEKFLKVYPELLKRLDDVSNDVRMAAASALLTWLKCIESVDGKSAYQSSVQFLYRELLVHLDDPESAIQDTVLEVLKEGSVLFPDVLVRETEAVVHKHRSATYCEQLLQHMQTMAAAR

Target ORF information:

RefSeq Version	NM_001134857.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus dynein, axonemal, assembly factor 5 (Dnaaf5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001134857.1

ORF Insert Sequence:

ATGGCGGCGC CGGCGGAGGC GGAGGTGGCC GCGGGCTTCG CCGAGGCGGA TGAAGCAGCG 
GAACTGACCC GCGCGCTAGG TCGTCTGTTA CCGGGACTGG AGACCGAAAG TAAGCTGGGG 
CGGCGGCGCG CGCTGGAAGC TCTGGAGCAC GCACTGGAAG AGGCGGTGCG GCCCGGAACG 
GATTCCGCTG CCTTCCAGGG CCCATGGGCT CGTCTGCTGC TGCCTCGCCT GCTGCGACTT 
CTGACCGACC CAGCCGAGGG CTGCCGCGCG CTGGCTGCTC ACCTGCTGGA CTTAGGCCTT 
CGCCGTGCCG CGCGGCCCCG CGATGCTCTG CCGCGCCTGT TGCCCGCGCT CACCGCACGC 
CTTGCCCGAC CAGAATCCAC GCGGCCACCG CCTGAACCCT GCGAAGAGCT GCGTCTTGCC 
CTCGTGCAAC TGCTTCACCT GGCCGTGGAC CTGGGCGGCG CCGCGCTCGC GCCCCACCTG 
GACGACGCGG TGCGCGCGTT GCGGGGTGCG CTGCTCGACC CCTTCGCCGC GGTGCGCCGG 
GAGGGTTGCG AGTGCGCAGC CGCTCTGGCG CGTGCCATTC CGGAGCACTT CCACATGCAG 
TCGGAGTCCC TCATAGGCCC CCTGATGCAG ACCATCTCCC ACCAGCACTG GAAGGTCCGG 
GTAGCTGTCA TCGAAGCCAC AGGCACTGTA ATCCAGTTTG GCAATGGGAA TTCGGTGGAT 
GATGTTCTTT CCCACTTTGC TCAAAGACTC TTCGATGATG TCCCTCAGGT CCGGCAGGCA 
GTGACCTCGG TGGTGGGGGG ATGGCTGCTC AATCTCCGTG ACCGATACTC CTTCTTTCAC 
AAACTCACCC CACTGTTACT CAGCAGCTTC AGTGACGAAA TGCCTGAGGT CCGACAGACA 
GCCACCAGCC TCTGGGAGAA GGTCGGGCTG CAGTGGCAGG AGGAAAATGA GGCAGATCTA 
AAGGACAAGC TGGACTTTGC TTCACCTCCT CCTCCTAACT ACCCTGAGCA CGAGAGCCGT 
CCTGGCCTGG GCTGCCGAGA GCTGGTCTTC AGGAACCTCT CCAAGGTGCT TCCTGCCATC 
TGTCACGATA TCACCGACTG GGTGGTGGGG ACCCGGCTGA AGGCAGCGCA GCTGCTGCCG 
GTGCTGCTGC TGCATGCAGA GGACCACATC ACACAGCACC TGGAGATCGT CCTCAGAACC 
CTGCATCAGG CGTGCACCGA TGAGGAGAAG GCTGTGGTCA GCAGCTGCGT GAGAGCCGCA 
GAGCTCATTG GCACCTTCGT CAGCCCAGAA GTATTTCTGA AACTGATCTT AGCCATGCTG 
AAGAAAGCAC CCTCGGCCTC TGGCCTCTTG ATCCTTGCTT CTGTCATCCG AGGCTGTCCC 
CGCAATGCCC TTCAGCCACA CCTCAAGGTC ATAGCCACAG AGCTGGCCCA GGAGCACATC 
TGCCAGGGGT CTGAGAATAA TAGCTACCTG GAGCATCTGC TGCTGTGTGT GCAGACTCTG 
CTGTCTGTGT GTCAGGAAGA CTGCTGTGCT GCCAGCCTGC AGCTCTCGGA GGTGCTGGTG 
ACAATAATGG CAGTCTCAGA TGCCGTGAGT CTTGAGAAGA AGGCTCAGAA GACCATGGAC 
ACCCTGGCTG AGGTGGAGGA TATCCCCAGC AGTCAGGACC TCTACCGCAA GCATCTGGGT 
GCACTACTGG AACGGCTGAC AGCCTCTCAT GGCGAGTGGA CGGTGCACTC TGTGCAGCTG 
CTGAAGTTCA CTGTGCTCCT CACCCAGGCA GGCCCAGCTG TGGGAGAGGC TCTGCAGCAT 
GTGATCCCCA CTCTCAGGGC CTGTCTCCAG CCCACCACAG ACCCACACAT GCGCCTGAAG 
CTCTTCTCTA TCCTGTCTTC ATTGCTGCTG AGGCCTAAGG ACACCGTCGA CTCTCAGGGA 
CAGTTCCATG GCTACTTGGA TATGGTAATA AATGACATCC TAGCCCCCAA CCTACAGTGG 
CATGCAGGCA GGACAGCTGC AGCCATCCGG ACGGCCACCA TATCCTGTCT GTGGGCACTT 
ATCAGCAGTG ACATCTTATC GGCCAAGCAG GTTCAAGAGG CACAGGAGAC CCTGATGCCA 
CAAGTGCTGG CCACGCTGGA GGACGACTCC CAGACCACAC GCCTGATCTC CTGCCGTATC 
ATCAGCAGGT TCCTCAAGAA CTCAGGGGAT ACCATGGAGC CAGAGAAATT TCTCAAGGTC 
TATCCTGAGC TCTTGAAGCG CCTAGATGAT GTCTCCAATG ACGTGAGAAT GGCAGCTGCT 
TCCGCCTTGC TCACCTGGCT GAAGTGCATT GAGAGCGTGG ATGGGAAATC CGCCTATCAG 
AGCAGCGTTC AGTTCCTGTA CAGGGAGCTG CTGGTCCACC TGGATGACCC AGAAAGTGCC 
ATCCAAGACA CTGTGCTAGA AGTCCTCAAG GAGGGCAGCG TCCTGTTTCC AGATGTGCTG 
GTGCGGGAGA CGGAGGCTGT TGTCCACAAA CACCGCTCAG CTACCTACTG CGAGCAGCTG 
CTGCAGCATA TGCAAACCAT GGCTGCCGCT CGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

HEATR2 plays a conserved role in assembly of the ciliary motile apparatus.
PLoS genetics10(9)e1004577(2014 Sep)
Diggle CP,Moore DJ,Mali G,zur Lage P,Ait-Lounis A,Schmidts M,Shoemark A,Garcia Munoz A,Halachev MR,Gautier P,Yeyati PL,Bonthron DT,Carr IM,Hayward B,Markham AF,Hope JE,von Kriegsheim A,Mitchison HM,Jackson IJ,Durand B,Reith W,Sheridan E,Jarman AP,Mill P

Whole-exome capture and sequencing identifies HEATR2 mutation as a cause of primary ciliary dyskinesia.
American journal of human genetics91(4)685-93(2012 Oct)
Horani A,Druley TE,Zariwala MA,Patel AC,Levinson BT,Van Arendonk LG,Thornton KC,Giacalone JC,Albee AJ,Wilson KS,Turner EH,Nickerson DA,Shendure J,Bayly PV,Leigh MW,Knowles MR,Brody SL,Dutcher SK,Ferkol TW

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Dnaaf5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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