Lrrc8b cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Lrrc8b
Entrez Gene ID	433926
Full Name	leucine rich repeat containing 8 family, member B
Synonyms	R75581,Ta-lrrp,mKIAA0231
General protein information	Preferred Names leucine rich repeat containing 8 family, member B Names volume-regulated anion channel subunit LRRC8B T-cell activation leucine repeat-rich protein leucine-rich repeat-containing protein 8B
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 E5

mRNA and Protein(s)

mRNA	Protein	Name
XM_030254651.1	XP_030110511.1	volume-regulated anion channel subunit LRRC8B isoform X1
XM_030254653.1	XP_030110513.1	volume-regulated anion channel subunit LRRC8B isoform X1
XM_011249508.2	XP_011247810.1	volume-regulated anion channel subunit LRRC8B isoform X1
NM_001033550.3	NP_001028722.1	volume-regulated anion channel subunit LRRC8B
XM_030254652.1	XP_030110512.1	volume-regulated anion channel subunit LRRC8B isoform X1
NM_001033550.2	NP_001028722.1	volume-regulated anion channel subunit LRRC8B
XM_011249508.3	XP_011247810.1	volume-regulated anion channel subunit LRRC8B isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	LRRC8B	NP_056165.1
Rattus norvegicus (Norway rat)	Lrrc8b	NP_001100674.1
Gallus gallus (chicken)	LRRC8B	XP_004936694.1
	LRRC8B	XP_002801670.1
Pan troglodytes (chimpanzee)	LRRC8B	XP_001150409.1
Bos taurus (cattle)	LRRC8B	XP_005204398.1
Mus musculus (house mouse)	Lrrc8b	NP_001028722.1
Canis lupus familiaris (dog)	LRRC8B	XP_005622010.1
Xenopus tropicalis (tropical clawed frog)	lrrc8b	XP_002941172.1

Gene Ontology
Bibliography

Related articles in PubMed

Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium.
Gaudet P, Livstone MS, Lewis SE, Thomas PD
Briefings in bioinformatics12(5)449-62(2011 Sep)

A high-resolution anatomical atlas of the transcriptome in the mouse embryo.
Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, Magen A, Canidio E, Pagani M, Peluso I, Lin-Marq N, Koch M, Bilio M, Cantiello I, Verde R, De Masi C, Bianchi SA, Cicchini J, Perroud E, Mehmeti S, Dagand E, Schrinner S, N?rnberger A, Schmidt K, Metz K, Zwingmann C, Brieske N, Springer C, Hernandez AM, Herzog S, Grabbe F, Sieverding C, Fischer B, Schrader K, Brockmeyer M, Dettmer S, Helbig C, Alunni V, Battaini MA, Mura C, Henrichsen CN, Garcia-Lopez R, Echevarria D, Puelles E, Garcia-Calero E, Kruse S, Uhr M, Kauck C, Feng G, Milyaev N, Ong CK, Kumar L, Lam M, Semple CA, Gyenesei A, Mundlos S, Radelof U, Lehrach H, Sarmientos P, Reymond A, Davidson DR, Doll? P, Antonarakis SE, Yaspo ML, Martinez S, Baldock RA, Eichele G, Ballabio A
PLoS biology9(1)e1000582(2011 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Lrrc8b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Lrrc8b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu19951	NM_001033550.3 Latest version!	Mus musculus leucine rich repeat containing 8 family, member B (Lrrc8b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu19951	XM_030254653.1 Latest version!	Mus musculus leucine rich repeat containing 8 family, member B (Lrrc8b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu19951	XM_030254652.1 Latest version!	Mus musculus leucine rich repeat containing 8 family, member B (Lrrc8b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu19951	XM_030254651.1 Latest version!	Mus musculus leucine rich repeat containing 8 family, member B (Lrrc8b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu19951	XM_011249508.3 Latest version!	Mus musculus leucine rich repeat containing 8 family, member B (Lrrc8b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OMu19951	NM_001033550.2	Mus musculus leucine rich repeat containing 8 family, member B (Lrrc8b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu19951	XM_011249508.2	Mus musculus leucine rich repeat containing 8 family, member B (Lrrc8b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Lrrc8b ( NM_001033550.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001028722.1 Mus musculus leucine rich repeat containing 8 family, member B (Lrrc8b), mRNA.

Price & Availability↑

CloneID	OMu19951
Clone ID Related Accession (Same CDS sequence)	XM_030254651.1 , XM_030254653.1 , XM_011249508.2 , NM_001033550.3 , XM_030254652.1 , NM_001033550.2 , XM_011249508.3
Accession Version	NM_001033550.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2412bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Mus musculus(house mouse)
Product	volume-regulated anion channel subunit LRRC8B
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK083378.1 and AC138265.9. On Feb 13, 2018 this sequence version replaced NM_001033550.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK083378.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849377 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATTACAC TAACAGAGTT AAAATGCTTA GCGGACGCCC AGTCATCCTA CCACATACTG 
AAGCCGTGGT GGGATGTCTT CTGGTATTAC ATCACCCTCA TCATGCTGTT GGTGGCTGTG 
CTGGCCGGAG CCCTGCAGCT CACCCAGAGC AGGGTTCTCT GCTGTCTTCC CTGCAAGGTG 
GAATTTGACA ATCAGTGCGC TGTGCCTTGG GACCTCCTGA AAGGCAGCGA GAACGCATCT 
TCTAACTCGG GCCTGCTGCT CCCGCTGCCA CTCCGGATCC AGAACGACCT CCACCGGCAG 
CAGTACTCCT ACATCGATGC TGTCTGCTAC GAGAAACAGC TCCATTGGTT CGCCAAGTTT 
TTCCCCTATC TGGTGCTTCT GCACACCCTC ATCTTCGCAG CCTGCAGCAA CTTCTGGCTT 
CACTACCCCA GCACCAGCTC GAGGCTGGAA CACTTTGTGT CCATCCTCCA CAAGTGCTTC 
GACTCTCCGT GGACCACACG CGCCCTGTCA GAGACAGTGG CGGAACAGTC AGTGAGGCCC 
CTGAAGCTCT CCAAGTCCAA AACCTTGCTC TCAACCTCAG GGGGCTCCGC CGACATCGAC 
GCCAGCAAGC AGTCATTGCC CTACCCACAG CCAGGCTTGG AGTCGCCTGG CATAGAAAGC 
CCAACTTCTA GCGTCCTGGA CAAGAAGGAG GGCGAACAAG CCAAGGCCAT CTTTGAAAAA 
GTGAAGAGGT TCCGCCTGCA CGTGGAGCAG AGGGATATCA TCTACCGGGT GTACCTGAAG 
CAGATAATCG TTAAAGTCAT CCTGTTTGTC CTTATTATAA CCTACGTCCC CTACTTTCTC 
AGCTACATAA CTCTTGAAAT CGACTGTTCG ATCGACGTGC AGGCATTTAC AGGCTATAAG 
CGCTACCAGT GTGTCTACTC CTTGGCAGAA ATCTTCAAGG TCCTAGCTTC CTTTTACGTC 
ATTCTGGTGA TGCTTTACGG CCTCACGTCC TCCTACAGCC TGTGGTGGAT GCTGAGGAGT 
TCCTTGAAGC AGTACTCCTT TGAGGCTCTC AGGGAGAAAA GCAACTACAG CGACATCCCC 
GATGTCAAGA ACGACTTTGC CTTCATCCTC CACCTGGCCG ACCAGTACGA CCCCCTGTAC 
TCTAAGCGCT TCTCCATCTT CCTGTCGGAG GTCAGCGAGA ACAAACTGAA ACAGATCAAC 
CTCAACAACG AGTGGACCGT GGAGAGACTG AAGAGCAAGC TCGTGAAAAA CTCGCAGGAC 
AAGGTCGAGC TGCATCTCTT CATGCTCAAC GGTCTCCCGG ATAACGTCTT TGAGCTGACG 
GAGATGGAAG TGCTGAGCCT GGAGCTGATC CCGGAAGTCA AGCTGCCGGC TGCCGTCGCT 
CAGCTCGTCA ACCTCAGGGA GCTCCACGTG TACCATTCGT CCCTGGTGGT CGACCACCCC 
GCCCTGGCCT TTCTAGAGGA GAACTTAAGA ATCCTCCGCC TGAAATTTAC CGAGATGGGG 
AAGATCCCAC GCTGGGTGTT CCACCTGAAG AACCTCAAGG AGCTGTACCT GTCGGGCTGC 
GTTCTCCCGG AGCAGCTGAG CAGCTTGCAC CTGGAGGGCT TCCAGGACTT GAAGAACCTG 
AGGACCCTCT ACTTGAAGAG CAGCCTGTCG CGGATCCCTC AGGTGGTGAC AGACCTGCTG 
CCCTCCCTGC AGAAGCTGTC CCTTGATAAT GAGGGAAGCA AACTGGTTGT GCTGAACAAC 
CTGAAGAAGA TGGTCAATCT GAAAAGCCTG GAGCTGCTCA GCTGCGACCT GGAGCGCATC 
CCCCACTCCA TTTTCAGCCT GAACAACCTG CACGAGCTGG ACCTGAAGGA GAACAACCTC 
AAGACCGTAG AGGAGATCAT CAGCTTCCAG CACCTCCCGA GCCTCTCCTG CCTGAAGCTC 
TGGCACAATA ACATCGCTTA CATCCCCGCG CAGATCGGAG CGCTGTCTAA CCTGGAGCAG 
CTCTTCCTGG GTCACAACAA CATCGAGAGT CTCCCTCTGC AGCTTTTTCT ATGCACCAAA 
CTACACTACT TGGATCTAAG CTATAACCAT CTGACCTTCA TTCCCGAGGA AATCCAGTAC 
CTGACCAATC TGCAGTACTT TGCTGTGACC AACAATAACA TTGAGATGCT ACCAGACGGG 
CTGTTCCAAT GCAAGAAGCT TCAGTGTCTA CTCCTGGGAA GAAATAGCCT AACAGATTTG 
TCCCCTCTGG TGGGGGAGCT GTCAAACCTC ACCCACCTGG AACTCACTGG TAACTACCTG 
GAGACACTGC CTGTGGAGCT GGAAGGGTGT CAGTCTCTGA AACGGAGCTG TCTGATTGTC 
GAGGACAGTT TGCTCAATTC CCTCCCGCTT CCCGTGGCAG AACGCTTGCA GACCTGCTTA 
GATAAATGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001028722.1
CDS	316..2727
Translation	MITLTELKCLADAQSSYHILKPWWDVFWYYITLIMLLVAVLAGALQLTQSRVLCCLPCKVEFDNQCAVPWDLLKGSENASSNSGLLLPLPLRIQNDLHRQQYSYIDAVCYEKQLHWFAKFFPYLVLLHTLIFAACSNFWLHYPSTSSRLEHFVSILHKCFDSPWTTRALSETVAEQSVRPLKLSKSKTLLSTSGGSADIDASKQSLPYPQPGLESPGIESPTSSVLDKKEGEQAKAIFEKVKRFRLHVEQRDIIYRVYLKQIIVKVILFVLIITYVPYFLSYITLEIDCSIDVQAFTGYKRYQCVYSLAEIFKVLASFYVILVMLYGLTSSYSLWWMLRSSLKQYSFEALREKSNYSDIPDVKNDFAFILHLADQYDPLYSKRFSIFLSEVSENKLKQINLNNEWTVERLKSKLVKNSQDKVELHLFMLNGLPDNVFELTEMEVLSLELIPEVKLPAAVAQLVNLRELHVYHSSLVVDHPALAFLEENLRILRLKFTEMGKIPRWVFHLKNLKELYLSGCVLPEQLSSLHLEGFQDLKNLRTLYLKSSLSRIPQVVTDLLPSLQKLSLDNEGSKLVVLNNLKKMVNLKSLELLSCDLERIPHSIFSLNNLHELDLKENNLKTVEEIISFQHLPSLSCLKLWHNNIAYIPAQIGALSNLEQLFLGHNNIESLPLQLFLCTKLHYLDLSYNHLTFIPEEIQYLTNLQYFAVTNNNIEMLPDGLFQCKKLQCLLLGRNSLTDLSPLVGELSNLTHLELTGNYLETLPVELEGCQSLKRSCLIVEDSLLNSLPLPVAERLQTCLDKC

Target ORF information:

RefSeq Version	NM_001033550.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus leucine rich repeat containing 8 family, member B (Lrrc8b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001033550.3

ORF Insert Sequence:

ATGATTACAC TAACAGAGTT AAAATGCTTA GCGGACGCCC AGTCATCCTA CCACATACTG 
AAGCCGTGGT GGGATGTCTT CTGGTATTAC ATCACCCTCA TCATGCTGTT GGTGGCTGTG 
CTGGCCGGAG CCCTGCAGCT CACCCAGAGC AGGGTTCTCT GCTGTCTTCC CTGCAAGGTG 
GAATTTGACA ATCAGTGCGC TGTGCCTTGG GACCTCCTGA AAGGCAGCGA GAACGCATCT 
TCTAACTCGG GCCTGCTGCT CCCGCTGCCA CTCCGGATCC AGAACGACCT CCACCGGCAG 
CAGTACTCCT ACATCGATGC TGTCTGCTAC GAGAAACAGC TCCATTGGTT CGCCAAGTTT 
TTCCCCTATC TGGTGCTTCT GCACACCCTC ATCTTCGCAG CCTGCAGCAA CTTCTGGCTT 
CACTACCCCA GCACCAGCTC GAGGCTGGAA CACTTTGTGT CCATCCTCCA CAAGTGCTTC 
GACTCTCCGT GGACCACACG CGCCCTGTCA GAGACAGTGG CGGAACAGTC AGTGAGGCCC 
CTGAAGCTCT CCAAGTCCAA AACCTTGCTC TCAACCTCAG GGGGCTCCGC CGACATCGAC 
GCCAGCAAGC AGTCATTGCC CTACCCACAG CCAGGCTTGG AGTCGCCTGG CATAGAAAGC 
CCAACTTCTA GCGTCCTGGA CAAGAAGGAG GGCGAACAAG CCAAGGCCAT CTTTGAAAAA 
GTGAAGAGGT TCCGCCTGCA CGTGGAGCAG AGGGATATCA TCTACCGGGT GTACCTGAAG 
CAGATAATCG TTAAAGTCAT CCTGTTTGTC CTTATTATAA CCTACGTCCC CTACTTTCTC 
AGCTACATAA CTCTTGAAAT CGACTGTTCG ATCGACGTGC AGGCATTTAC AGGCTATAAG 
CGCTACCAGT GTGTCTACTC CTTGGCAGAA ATCTTCAAGG TCCTAGCTTC CTTTTACGTC 
ATTCTGGTGA TGCTTTACGG CCTCACGTCC TCCTACAGCC TGTGGTGGAT GCTGAGGAGT 
TCCTTGAAGC AGTACTCCTT TGAGGCTCTC AGGGAGAAAA GCAACTACAG CGACATCCCC 
GATGTCAAGA ACGACTTTGC CTTCATCCTC CACCTGGCCG ACCAGTACGA CCCCCTGTAC 
TCTAAGCGCT TCTCCATCTT CCTGTCGGAG GTCAGCGAGA ACAAACTGAA ACAGATCAAC 
CTCAACAACG AGTGGACCGT GGAGAGACTG AAGAGCAAGC TCGTGAAAAA CTCGCAGGAC 
AAGGTCGAGC TGCATCTCTT CATGCTCAAC GGTCTCCCGG ATAACGTCTT TGAGCTGACG 
GAGATGGAAG TGCTGAGCCT GGAGCTGATC CCGGAAGTCA AGCTGCCGGC TGCCGTCGCT 
CAGCTCGTCA ACCTCAGGGA GCTCCACGTG TACCATTCGT CCCTGGTGGT CGACCACCCC 
GCCCTGGCCT TTCTAGAGGA GAACTTAAGA ATCCTCCGCC TGAAATTTAC CGAGATGGGG 
AAGATCCCAC GCTGGGTGTT CCACCTGAAG AACCTCAAGG AGCTGTACCT GTCGGGCTGC 
GTTCTCCCGG AGCAGCTGAG CAGCTTGCAC CTGGAGGGCT TCCAGGACTT GAAGAACCTG 
AGGACCCTCT ACTTGAAGAG CAGCCTGTCG CGGATCCCTC AGGTGGTGAC AGACCTGCTG 
CCCTCCCTGC AGAAGCTGTC CCTTGATAAT GAGGGAAGCA AACTGGTTGT GCTGAACAAC 
CTGAAGAAGA TGGTCAATCT GAAAAGCCTG GAGCTGCTCA GCTGCGACCT GGAGCGCATC 
CCCCACTCCA TTTTCAGCCT GAACAACCTG CACGAGCTGG ACCTGAAGGA GAACAACCTC 
AAGACCGTAG AGGAGATCAT CAGCTTCCAG CACCTCCCGA GCCTCTCCTG CCTGAAGCTC 
TGGCACAATA ACATCGCTTA CATCCCCGCG CAGATCGGAG CGCTGTCTAA CCTGGAGCAG 
CTCTTCCTGG GTCACAACAA CATCGAGAGT CTCCCTCTGC AGCTTTTTCT ATGCACCAAA 
CTACACTACT TGGATCTAAG CTATAACCAT CTGACCTTCA TTCCCGAGGA AATCCAGTAC 
CTGACCAATC TGCAGTACTT TGCTGTGACC AACAATAACA TTGAGATGCT ACCAGACGGG 
CTGTTCCAAT GCAAGAAGCT TCAGTGTCTA CTCCTGGGAA GAAATAGCCT AACAGATTTG 
TCCCCTCTGG TGGGGGAGCT GTCAAACCTC ACCCACCTGG AACTCACTGG TAACTACCTG 
GAGACACTGC CTGTGGAGCT GGAAGGGTGT CAGTCTCTGA AACGGAGCTG TCTGATTGTC 
GAGGACAGTT TGCTCAATTC CCTCCCGCTT CCCGTGGCAG AACGCTTGCA GACCTGCTTA 
GATAAATGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lrrc8b ( NM_001033550.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001028722.1 Mus musculus leucine rich repeat containing 8 family, member B (Lrrc8b), mRNA.

Price & Availability↑

CloneID	OMu19951
Clone ID Related Accession (Same CDS sequence)	XM_030254651.1 , XM_030254653.1 , XM_011249508.2 , NM_001033550.3 , XM_030254652.1 , NM_001033550.2 , XM_011249508.3
Accession Version	NM_001033550.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2412bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-28
Organism	Mus musculus(house mouse)
Product	volume-regulated anion channel subunit LRRC8B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AK083378.1. On Apr 10, 2007 this sequence version replaced NM_001033550.1. ##Evidence-Data-START## Transcript exon combination :: AK083378.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849377 [ECO:0000348] ##Evidence-Data-END##

ATGATTACAC TAACAGAGTT AAAATGCTTA GCGGACGCCC AGTCATCCTA CCACATACTG 
AAGCCGTGGT GGGATGTCTT CTGGTATTAC ATCACCCTCA TCATGCTGTT GGTGGCTGTG 
CTGGCCGGAG CCCTGCAGCT CACCCAGAGC AGGGTTCTCT GCTGTCTTCC CTGCAAGGTG 
GAATTTGACA ATCAGTGCGC TGTGCCTTGG GACCTCCTGA AAGGCAGCGA GAACGCATCT 
TCTAACTCGG GCCTGCTGCT CCCGCTGCCA CTCCGGATCC AGAACGACCT CCACCGGCAG 
CAGTACTCCT ACATCGATGC TGTCTGCTAC GAGAAACAGC TCCATTGGTT CGCCAAGTTT 
TTCCCCTATC TGGTGCTTCT GCACACCCTC ATCTTCGCAG CCTGCAGCAA CTTCTGGCTT 
CACTACCCCA GCACCAGCTC GAGGCTGGAA CACTTTGTGT CCATCCTCCA CAAGTGCTTC 
GACTCTCCGT GGACCACACG CGCCCTGTCA GAGACAGTGG CGGAACAGTC AGTGAGGCCC 
CTGAAGCTCT CCAAGTCCAA AACCTTGCTC TCAACCTCAG GGGGCTCCGC CGACATCGAC 
GCCAGCAAGC AGTCATTGCC CTACCCACAG CCAGGCTTGG AGTCGCCTGG CATAGAAAGC 
CCAACTTCTA GCGTCCTGGA CAAGAAGGAG GGCGAACAAG CCAAGGCCAT CTTTGAAAAA 
GTGAAGAGGT TCCGCCTGCA CGTGGAGCAG AGGGATATCA TCTACCGGGT GTACCTGAAG 
CAGATAATCG TTAAAGTCAT CCTGTTTGTC CTTATTATAA CCTACGTCCC CTACTTTCTC 
AGCTACATAA CTCTTGAAAT CGACTGTTCG ATCGACGTGC AGGCATTTAC AGGCTATAAG 
CGCTACCAGT GTGTCTACTC CTTGGCAGAA ATCTTCAAGG TCCTAGCTTC CTTTTACGTC 
ATTCTGGTGA TGCTTTACGG CCTCACGTCC TCCTACAGCC TGTGGTGGAT GCTGAGGAGT 
TCCTTGAAGC AGTACTCCTT TGAGGCTCTC AGGGAGAAAA GCAACTACAG CGACATCCCC 
GATGTCAAGA ACGACTTTGC CTTCATCCTC CACCTGGCCG ACCAGTACGA CCCCCTGTAC 
TCTAAGCGCT TCTCCATCTT CCTGTCGGAG GTCAGCGAGA ACAAACTGAA ACAGATCAAC 
CTCAACAACG AGTGGACCGT GGAGAGACTG AAGAGCAAGC TCGTGAAAAA CTCGCAGGAC 
AAGGTCGAGC TGCATCTCTT CATGCTCAAC GGTCTCCCGG ATAACGTCTT TGAGCTGACG 
GAGATGGAAG TGCTGAGCCT GGAGCTGATC CCGGAAGTCA AGCTGCCGGC TGCCGTCGCT 
CAGCTCGTCA ACCTCAGGGA GCTCCACGTG TACCATTCGT CCCTGGTGGT CGACCACCCC 
GCCCTGGCCT TTCTAGAGGA GAACTTAAGA ATCCTCCGCC TGAAATTTAC CGAGATGGGG 
AAGATCCCAC GCTGGGTGTT CCACCTGAAG AACCTCAAGG AGCTGTACCT GTCGGGCTGC 
GTTCTCCCGG AGCAGCTGAG CAGCTTGCAC CTGGAGGGCT TCCAGGACTT GAAGAACCTG 
AGGACCCTCT ACTTGAAGAG CAGCCTGTCG CGGATCCCTC AGGTGGTGAC AGACCTGCTG 
CCCTCCCTGC AGAAGCTGTC CCTTGATAAT GAGGGAAGCA AACTGGTTGT GCTGAACAAC 
CTGAAGAAGA TGGTCAATCT GAAAAGCCTG GAGCTGCTCA GCTGCGACCT GGAGCGCATC 
CCCCACTCCA TTTTCAGCCT GAACAACCTG CACGAGCTGG ACCTGAAGGA GAACAACCTC 
AAGACCGTAG AGGAGATCAT CAGCTTCCAG CACCTCCCGA GCCTCTCCTG CCTGAAGCTC 
TGGCACAATA ACATCGCTTA CATCCCCGCG CAGATCGGAG CGCTGTCTAA CCTGGAGCAG 
CTCTTCCTGG GTCACAACAA CATCGAGAGT CTCCCTCTGC AGCTTTTTCT ATGCACCAAA 
CTACACTACT TGGATCTAAG CTATAACCAT CTGACCTTCA TTCCCGAGGA AATCCAGTAC 
CTGACCAATC TGCAGTACTT TGCTGTGACC AACAATAACA TTGAGATGCT ACCAGACGGG 
CTGTTCCAAT GCAAGAAGCT TCAGTGTCTA CTCCTGGGAA GAAATAGCCT AACAGATTTG 
TCCCCTCTGG TGGGGGAGCT GTCAAACCTC ACCCACCTGG AACTCACTGG TAACTACCTG 
GAGACACTGC CTGTGGAGCT GGAAGGGTGT CAGTCTCTGA AACGGAGCTG TCTGATTGTC 
GAGGACAGTT TGCTCAATTC CCTCCCGCTT CCCGTGGCAG AACGCTTGCA GACCTGCTTA 
GATAAATGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001028722.1
CDS	316..2727
Translation	MITLTELKCLADAQSSYHILKPWWDVFWYYITLIMLLVAVLAGALQLTQSRVLCCLPCKVEFDNQCAVPWDLLKGSENASSNSGLLLPLPLRIQNDLHRQQYSYIDAVCYEKQLHWFAKFFPYLVLLHTLIFAACSNFWLHYPSTSSRLEHFVSILHKCFDSPWTTRALSETVAEQSVRPLKLSKSKTLLSTSGGSADIDASKQSLPYPQPGLESPGIESPTSSVLDKKEGEQAKAIFEKVKRFRLHVEQRDIIYRVYLKQIIVKVILFVLIITYVPYFLSYITLEIDCSIDVQAFTGYKRYQCVYSLAEIFKVLASFYVILVMLYGLTSSYSLWWMLRSSLKQYSFEALREKSNYSDIPDVKNDFAFILHLADQYDPLYSKRFSIFLSEVSENKLKQINLNNEWTVERLKSKLVKNSQDKVELHLFMLNGLPDNVFELTEMEVLSLELIPEVKLPAAVAQLVNLRELHVYHSSLVVDHPALAFLEENLRILRLKFTEMGKIPRWVFHLKNLKELYLSGCVLPEQLSSLHLEGFQDLKNLRTLYLKSSLSRIPQVVTDLLPSLQKLSLDNEGSKLVVLNNLKKMVNLKSLELLSCDLERIPHSIFSLNNLHELDLKENNLKTVEEIISFQHLPSLSCLKLWHNNIAYIPAQIGALSNLEQLFLGHNNIESLPLQLFLCTKLHYLDLSYNHLTFIPEEIQYLTNLQYFAVTNNNIEMLPDGLFQCKKLQCLLLGRNSLTDLSPLVGELSNLTHLELTGNYLETLPVELEGCQSLKRSCLIVEDSLLNSLPLPVAERLQTCLDKC

Target ORF information:

RefSeq Version	NM_001033550.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus leucine rich repeat containing 8 family, member B (Lrrc8b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001033550.2

ORF Insert Sequence:

ATGATTACAC TAACAGAGTT AAAATGCTTA GCGGACGCCC AGTCATCCTA CCACATACTG 
AAGCCGTGGT GGGATGTCTT CTGGTATTAC ATCACCCTCA TCATGCTGTT GGTGGCTGTG 
CTGGCCGGAG CCCTGCAGCT CACCCAGAGC AGGGTTCTCT GCTGTCTTCC CTGCAAGGTG 
GAATTTGACA ATCAGTGCGC TGTGCCTTGG GACCTCCTGA AAGGCAGCGA GAACGCATCT 
TCTAACTCGG GCCTGCTGCT CCCGCTGCCA CTCCGGATCC AGAACGACCT CCACCGGCAG 
CAGTACTCCT ACATCGATGC TGTCTGCTAC GAGAAACAGC TCCATTGGTT CGCCAAGTTT 
TTCCCCTATC TGGTGCTTCT GCACACCCTC ATCTTCGCAG CCTGCAGCAA CTTCTGGCTT 
CACTACCCCA GCACCAGCTC GAGGCTGGAA CACTTTGTGT CCATCCTCCA CAAGTGCTTC 
GACTCTCCGT GGACCACACG CGCCCTGTCA GAGACAGTGG CGGAACAGTC AGTGAGGCCC 
CTGAAGCTCT CCAAGTCCAA AACCTTGCTC TCAACCTCAG GGGGCTCCGC CGACATCGAC 
GCCAGCAAGC AGTCATTGCC CTACCCACAG CCAGGCTTGG AGTCGCCTGG CATAGAAAGC 
CCAACTTCTA GCGTCCTGGA CAAGAAGGAG GGCGAACAAG CCAAGGCCAT CTTTGAAAAA 
GTGAAGAGGT TCCGCCTGCA CGTGGAGCAG AGGGATATCA TCTACCGGGT GTACCTGAAG 
CAGATAATCG TTAAAGTCAT CCTGTTTGTC CTTATTATAA CCTACGTCCC CTACTTTCTC 
AGCTACATAA CTCTTGAAAT CGACTGTTCG ATCGACGTGC AGGCATTTAC AGGCTATAAG 
CGCTACCAGT GTGTCTACTC CTTGGCAGAA ATCTTCAAGG TCCTAGCTTC CTTTTACGTC 
ATTCTGGTGA TGCTTTACGG CCTCACGTCC TCCTACAGCC TGTGGTGGAT GCTGAGGAGT 
TCCTTGAAGC AGTACTCCTT TGAGGCTCTC AGGGAGAAAA GCAACTACAG CGACATCCCC 
GATGTCAAGA ACGACTTTGC CTTCATCCTC CACCTGGCCG ACCAGTACGA CCCCCTGTAC 
TCTAAGCGCT TCTCCATCTT CCTGTCGGAG GTCAGCGAGA ACAAACTGAA ACAGATCAAC 
CTCAACAACG AGTGGACCGT GGAGAGACTG AAGAGCAAGC TCGTGAAAAA CTCGCAGGAC 
AAGGTCGAGC TGCATCTCTT CATGCTCAAC GGTCTCCCGG ATAACGTCTT TGAGCTGACG 
GAGATGGAAG TGCTGAGCCT GGAGCTGATC CCGGAAGTCA AGCTGCCGGC TGCCGTCGCT 
CAGCTCGTCA ACCTCAGGGA GCTCCACGTG TACCATTCGT CCCTGGTGGT CGACCACCCC 
GCCCTGGCCT TTCTAGAGGA GAACTTAAGA ATCCTCCGCC TGAAATTTAC CGAGATGGGG 
AAGATCCCAC GCTGGGTGTT CCACCTGAAG AACCTCAAGG AGCTGTACCT GTCGGGCTGC 
GTTCTCCCGG AGCAGCTGAG CAGCTTGCAC CTGGAGGGCT TCCAGGACTT GAAGAACCTG 
AGGACCCTCT ACTTGAAGAG CAGCCTGTCG CGGATCCCTC AGGTGGTGAC AGACCTGCTG 
CCCTCCCTGC AGAAGCTGTC CCTTGATAAT GAGGGAAGCA AACTGGTTGT GCTGAACAAC 
CTGAAGAAGA TGGTCAATCT GAAAAGCCTG GAGCTGCTCA GCTGCGACCT GGAGCGCATC 
CCCCACTCCA TTTTCAGCCT GAACAACCTG CACGAGCTGG ACCTGAAGGA GAACAACCTC 
AAGACCGTAG AGGAGATCAT CAGCTTCCAG CACCTCCCGA GCCTCTCCTG CCTGAAGCTC 
TGGCACAATA ACATCGCTTA CATCCCCGCG CAGATCGGAG CGCTGTCTAA CCTGGAGCAG 
CTCTTCCTGG GTCACAACAA CATCGAGAGT CTCCCTCTGC AGCTTTTTCT ATGCACCAAA 
CTACACTACT TGGATCTAAG CTATAACCAT CTGACCTTCA TTCCCGAGGA AATCCAGTAC 
CTGACCAATC TGCAGTACTT TGCTGTGACC AACAATAACA TTGAGATGCT ACCAGACGGG 
CTGTTCCAAT GCAAGAAGCT TCAGTGTCTA CTCCTGGGAA GAAATAGCCT AACAGATTTG 
TCCCCTCTGG TGGGGGAGCT GTCAAACCTC ACCCACCTGG AACTCACTGG TAACTACCTG 
GAGACACTGC CTGTGGAGCT GGAAGGGTGT CAGTCTCTGA AACGGAGCTG TCTGATTGTC 
GAGGACAGTT TGCTCAATTC CCTCCCGCTT CCCGTGGCAG AACGCTTGCA GACCTGCTTA 
GATAAATGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lrrc8b ( XM_011249508.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011247810.1 Mus musculus leucine rich repeat containing 8 family, member B (Lrrc8b), transcript variant X1, mRNA.

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CloneID	OMu19951
Clone ID Related Accession (Same CDS sequence)	XM_030254651.1 , XM_030254653.1 , XM_011249508.2 , NM_001033550.3 , XM_030254652.1 , NM_001033550.2 , XM_011249508.3
Accession Version	XM_011249508.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2412bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	volume-regulated anion channel subunit LRRC8B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011249508.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTACAC TAACAGAGTT AAAATGCTTA GCGGACGCCC AGTCATCCTA CCACATACTG 
AAGCCGTGGT GGGATGTCTT CTGGTATTAC ATCACCCTCA TCATGCTGTT GGTGGCTGTG 
CTGGCCGGAG CCCTGCAGCT CACCCAGAGC AGGGTTCTCT GCTGTCTTCC CTGCAAGGTG 
GAATTTGACA ATCAGTGCGC TGTGCCTTGG GACCTCCTGA AAGGCAGCGA GAACGCATCT 
TCTAACTCGG GCCTGCTGCT CCCGCTGCCA CTCCGGATCC AGAACGACCT CCACCGGCAG 
CAGTACTCCT ACATCGATGC TGTCTGCTAC GAGAAACAGC TCCATTGGTT CGCCAAGTTT 
TTCCCCTATC TGGTGCTTCT GCACACCCTC ATCTTCGCAG CCTGCAGCAA CTTCTGGCTT 
CACTACCCCA GCACCAGCTC GAGGCTGGAA CACTTTGTGT CCATCCTCCA CAAGTGCTTC 
GACTCTCCGT GGACCACACG CGCCCTGTCA GAGACAGTGG CGGAACAGTC AGTGAGGCCC 
CTGAAGCTCT CCAAGTCCAA AACCTTGCTC TCAACCTCAG GGGGCTCCGC CGACATCGAC 
GCCAGCAAGC AGTCATTGCC CTACCCACAG CCAGGCTTGG AGTCGCCTGG CATAGAAAGC 
CCAACTTCTA GCGTCCTGGA CAAGAAGGAG GGCGAACAAG CCAAGGCCAT CTTTGAAAAA 
GTGAAGAGGT TCCGCCTGCA CGTGGAGCAG AGGGATATCA TCTACCGGGT GTACCTGAAG 
CAGATAATCG TTAAAGTCAT CCTGTTTGTC CTTATTATAA CCTACGTCCC CTACTTTCTC 
AGCTACATAA CTCTTGAAAT CGACTGTTCG ATCGACGTGC AGGCATTTAC AGGCTATAAG 
CGCTACCAGT GTGTCTACTC CTTGGCAGAA ATCTTCAAGG TCCTAGCTTC CTTTTACGTC 
ATTCTGGTGA TGCTTTACGG CCTCACGTCC TCCTACAGCC TGTGGTGGAT GCTGAGGAGT 
TCCTTGAAGC AGTACTCCTT TGAGGCTCTC AGGGAGAAAA GCAACTACAG CGACATCCCC 
GATGTCAAGA ACGACTTTGC CTTCATCCTC CACCTGGCCG ACCAGTACGA CCCCCTGTAC 
TCTAAGCGCT TCTCCATCTT CCTGTCGGAG GTCAGCGAGA ACAAACTGAA ACAGATCAAC 
CTCAACAACG AGTGGACCGT GGAGAGACTG AAGAGCAAGC TCGTGAAAAA CTCGCAGGAC 
AAGGTCGAGC TGCATCTCTT CATGCTCAAC GGTCTCCCGG ATAACGTCTT TGAGCTGACG 
GAGATGGAAG TGCTGAGCCT GGAGCTGATC CCGGAAGTCA AGCTGCCGGC TGCCGTCGCT 
CAGCTCGTCA ACCTCAGGGA GCTCCACGTG TACCATTCGT CCCTGGTGGT CGACCACCCC 
GCCCTGGCCT TTCTAGAGGA GAACTTAAGA ATCCTCCGCC TGAAATTTAC CGAGATGGGG 
AAGATCCCAC GCTGGGTGTT CCACCTGAAG AACCTCAAGG AGCTGTACCT GTCGGGCTGC 
GTTCTCCCGG AGCAGCTGAG CAGCTTGCAC CTGGAGGGCT TCCAGGACTT GAAGAACCTG 
AGGACCCTCT ACTTGAAGAG CAGCCTGTCG CGGATCCCTC AGGTGGTGAC AGACCTGCTG 
CCCTCCCTGC AGAAGCTGTC CCTTGATAAT GAGGGAAGCA AACTGGTTGT GCTGAACAAC 
CTGAAGAAGA TGGTCAATCT GAAAAGCCTG GAGCTGCTCA GCTGCGACCT GGAGCGCATC 
CCCCACTCCA TTTTCAGCCT GAACAACCTG CACGAGCTGG ACCTGAAGGA GAACAACCTC 
AAGACCGTAG AGGAGATCAT CAGCTTCCAG CACCTCCCGA GCCTCTCCTG CCTGAAGCTC 
TGGCACAATA ACATCGCTTA CATCCCCGCG CAGATCGGAG CGCTGTCTAA CCTGGAGCAG 
CTCTTCCTGG GTCACAACAA CATCGAGAGT CTCCCTCTGC AGCTTTTTCT ATGCACCAAA 
CTACACTACT TGGATCTAAG CTATAACCAT CTGACCTTCA TTCCCGAGGA AATCCAGTAC 
CTGACCAATC TGCAGTACTT TGCTGTGACC AACAATAACA TTGAGATGCT ACCAGACGGG 
CTGTTCCAAT GCAAGAAGCT TCAGTGTCTA CTCCTGGGAA GAAATAGCCT AACAGATTTG 
TCCCCTCTGG TGGGGGAGCT GTCAAACCTC ACCCACCTGG AACTCACTGG TAACTACCTG 
GAGACACTGC CTGTGGAGCT GGAAGGGTGT CAGTCTCTGA AACGGAGCTG TCTGATTGTC 
GAGGACAGTT TGCTCAATTC CCTCCCGCTT CCCGTGGCAG AACGCTTGCA GACCTGCTTA 
GATAAATGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011247810.1
CDS	1015..3426
Translation	MITLTELKCLADAQSSYHILKPWWDVFWYYITLIMLLVAVLAGALQLTQSRVLCCLPCKVEFDNQCAVPWDLLKGSENASSNSGLLLPLPLRIQNDLHRQQYSYIDAVCYEKQLHWFAKFFPYLVLLHTLIFAACSNFWLHYPSTSSRLEHFVSILHKCFDSPWTTRALSETVAEQSVRPLKLSKSKTLLSTSGGSADIDASKQSLPYPQPGLESPGIESPTSSVLDKKEGEQAKAIFEKVKRFRLHVEQRDIIYRVYLKQIIVKVILFVLIITYVPYFLSYITLEIDCSIDVQAFTGYKRYQCVYSLAEIFKVLASFYVILVMLYGLTSSYSLWWMLRSSLKQYSFEALREKSNYSDIPDVKNDFAFILHLADQYDPLYSKRFSIFLSEVSENKLKQINLNNEWTVERLKSKLVKNSQDKVELHLFMLNGLPDNVFELTEMEVLSLELIPEVKLPAAVAQLVNLRELHVYHSSLVVDHPALAFLEENLRILRLKFTEMGKIPRWVFHLKNLKELYLSGCVLPEQLSSLHLEGFQDLKNLRTLYLKSSLSRIPQVVTDLLPSLQKLSLDNEGSKLVVLNNLKKMVNLKSLELLSCDLERIPHSIFSLNNLHELDLKENNLKTVEEIISFQHLPSLSCLKLWHNNIAYIPAQIGALSNLEQLFLGHNNIESLPLQLFLCTKLHYLDLSYNHLTFIPEEIQYLTNLQYFAVTNNNIEMLPDGLFQCKKLQCLLLGRNSLTDLSPLVGELSNLTHLELTGNYLETLPVELEGCQSLKRSCLIVEDSLLNSLPLPVAERLQTCLDKC

Target ORF information:

RefSeq Version	XM_011249508.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus leucine rich repeat containing 8 family, member B (Lrrc8b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011249508.2

ORF Insert Sequence:

ATGATTACAC TAACAGAGTT AAAATGCTTA GCGGACGCCC AGTCATCCTA CCACATACTG 
AAGCCGTGGT GGGATGTCTT CTGGTATTAC ATCACCCTCA TCATGCTGTT GGTGGCTGTG 
CTGGCCGGAG CCCTGCAGCT CACCCAGAGC AGGGTTCTCT GCTGTCTTCC CTGCAAGGTG 
GAATTTGACA ATCAGTGCGC TGTGCCTTGG GACCTCCTGA AAGGCAGCGA GAACGCATCT 
TCTAACTCGG GCCTGCTGCT CCCGCTGCCA CTCCGGATCC AGAACGACCT CCACCGGCAG 
CAGTACTCCT ACATCGATGC TGTCTGCTAC GAGAAACAGC TCCATTGGTT CGCCAAGTTT 
TTCCCCTATC TGGTGCTTCT GCACACCCTC ATCTTCGCAG CCTGCAGCAA CTTCTGGCTT 
CACTACCCCA GCACCAGCTC GAGGCTGGAA CACTTTGTGT CCATCCTCCA CAAGTGCTTC 
GACTCTCCGT GGACCACACG CGCCCTGTCA GAGACAGTGG CGGAACAGTC AGTGAGGCCC 
CTGAAGCTCT CCAAGTCCAA AACCTTGCTC TCAACCTCAG GGGGCTCCGC CGACATCGAC 
GCCAGCAAGC AGTCATTGCC CTACCCACAG CCAGGCTTGG AGTCGCCTGG CATAGAAAGC 
CCAACTTCTA GCGTCCTGGA CAAGAAGGAG GGCGAACAAG CCAAGGCCAT CTTTGAAAAA 
GTGAAGAGGT TCCGCCTGCA CGTGGAGCAG AGGGATATCA TCTACCGGGT GTACCTGAAG 
CAGATAATCG TTAAAGTCAT CCTGTTTGTC CTTATTATAA CCTACGTCCC CTACTTTCTC 
AGCTACATAA CTCTTGAAAT CGACTGTTCG ATCGACGTGC AGGCATTTAC AGGCTATAAG 
CGCTACCAGT GTGTCTACTC CTTGGCAGAA ATCTTCAAGG TCCTAGCTTC CTTTTACGTC 
ATTCTGGTGA TGCTTTACGG CCTCACGTCC TCCTACAGCC TGTGGTGGAT GCTGAGGAGT 
TCCTTGAAGC AGTACTCCTT TGAGGCTCTC AGGGAGAAAA GCAACTACAG CGACATCCCC 
GATGTCAAGA ACGACTTTGC CTTCATCCTC CACCTGGCCG ACCAGTACGA CCCCCTGTAC 
TCTAAGCGCT TCTCCATCTT CCTGTCGGAG GTCAGCGAGA ACAAACTGAA ACAGATCAAC 
CTCAACAACG AGTGGACCGT GGAGAGACTG AAGAGCAAGC TCGTGAAAAA CTCGCAGGAC 
AAGGTCGAGC TGCATCTCTT CATGCTCAAC GGTCTCCCGG ATAACGTCTT TGAGCTGACG 
GAGATGGAAG TGCTGAGCCT GGAGCTGATC CCGGAAGTCA AGCTGCCGGC TGCCGTCGCT 
CAGCTCGTCA ACCTCAGGGA GCTCCACGTG TACCATTCGT CCCTGGTGGT CGACCACCCC 
GCCCTGGCCT TTCTAGAGGA GAACTTAAGA ATCCTCCGCC TGAAATTTAC CGAGATGGGG 
AAGATCCCAC GCTGGGTGTT CCACCTGAAG AACCTCAAGG AGCTGTACCT GTCGGGCTGC 
GTTCTCCCGG AGCAGCTGAG CAGCTTGCAC CTGGAGGGCT TCCAGGACTT GAAGAACCTG 
AGGACCCTCT ACTTGAAGAG CAGCCTGTCG CGGATCCCTC AGGTGGTGAC AGACCTGCTG 
CCCTCCCTGC AGAAGCTGTC CCTTGATAAT GAGGGAAGCA AACTGGTTGT GCTGAACAAC 
CTGAAGAAGA TGGTCAATCT GAAAAGCCTG GAGCTGCTCA GCTGCGACCT GGAGCGCATC 
CCCCACTCCA TTTTCAGCCT GAACAACCTG CACGAGCTGG ACCTGAAGGA GAACAACCTC 
AAGACCGTAG AGGAGATCAT CAGCTTCCAG CACCTCCCGA GCCTCTCCTG CCTGAAGCTC 
TGGCACAATA ACATCGCTTA CATCCCCGCG CAGATCGGAG CGCTGTCTAA CCTGGAGCAG 
CTCTTCCTGG GTCACAACAA CATCGAGAGT CTCCCTCTGC AGCTTTTTCT ATGCACCAAA 
CTACACTACT TGGATCTAAG CTATAACCAT CTGACCTTCA TTCCCGAGGA AATCCAGTAC 
CTGACCAATC TGCAGTACTT TGCTGTGACC AACAATAACA TTGAGATGCT ACCAGACGGG 
CTGTTCCAAT GCAAGAAGCT TCAGTGTCTA CTCCTGGGAA GAAATAGCCT AACAGATTTG 
TCCCCTCTGG TGGGGGAGCT GTCAAACCTC ACCCACCTGG AACTCACTGG TAACTACCTG 
GAGACACTGC CTGTGGAGCT GGAAGGGTGT CAGTCTCTGA AACGGAGCTG TCTGATTGTC 
GAGGACAGTT TGCTCAATTC CCTCCCGCTT CCCGTGGCAG AACGCTTGCA GACCTGCTTA 
GATAAATGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lrrc8b ( XM_030254651.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030110511.1 Mus musculus leucine rich repeat containing 8 family, member B (Lrrc8b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu19951
Clone ID Related Accession (Same CDS sequence)	XM_030254651.1 , XM_030254653.1 , XM_011249508.2 , NM_001033550.3 , XM_030254652.1 , NM_001033550.2 , XM_011249508.3
Accession Version	XM_030254651.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2412bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	volume-regulated anion channel subunit LRRC8B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTACAC TAACAGAGTT AAAATGCTTA GCGGACGCCC AGTCATCCTA CCACATACTG 
AAGCCGTGGT GGGATGTCTT CTGGTATTAC ATCACCCTCA TCATGCTGTT GGTGGCTGTG 
CTGGCCGGAG CCCTGCAGCT CACCCAGAGC AGGGTTCTCT GCTGTCTTCC CTGCAAGGTG 
GAATTTGACA ATCAGTGCGC TGTGCCTTGG GACCTCCTGA AAGGCAGCGA GAACGCATCT 
TCTAACTCGG GCCTGCTGCT CCCGCTGCCA CTCCGGATCC AGAACGACCT CCACCGGCAG 
CAGTACTCCT ACATCGATGC TGTCTGCTAC GAGAAACAGC TCCATTGGTT CGCCAAGTTT 
TTCCCCTATC TGGTGCTTCT GCACACCCTC ATCTTCGCAG CCTGCAGCAA CTTCTGGCTT 
CACTACCCCA GCACCAGCTC GAGGCTGGAA CACTTTGTGT CCATCCTCCA CAAGTGCTTC 
GACTCTCCGT GGACCACACG CGCCCTGTCA GAGACAGTGG CGGAACAGTC AGTGAGGCCC 
CTGAAGCTCT CCAAGTCCAA AACCTTGCTC TCAACCTCAG GGGGCTCCGC CGACATCGAC 
GCCAGCAAGC AGTCATTGCC CTACCCACAG CCAGGCTTGG AGTCGCCTGG CATAGAAAGC 
CCAACTTCTA GCGTCCTGGA CAAGAAGGAG GGCGAACAAG CCAAGGCCAT CTTTGAAAAA 
GTGAAGAGGT TCCGCCTGCA CGTGGAGCAG AGGGATATCA TCTACCGGGT GTACCTGAAG 
CAGATAATCG TTAAAGTCAT CCTGTTTGTC CTTATTATAA CCTACGTCCC CTACTTTCTC 
AGCTACATAA CTCTTGAAAT CGACTGTTCG ATCGACGTGC AGGCATTTAC AGGCTATAAG 
CGCTACCAGT GTGTCTACTC CTTGGCAGAA ATCTTCAAGG TCCTAGCTTC CTTTTACGTC 
ATTCTGGTGA TGCTTTACGG CCTCACGTCC TCCTACAGCC TGTGGTGGAT GCTGAGGAGT 
TCCTTGAAGC AGTACTCCTT TGAGGCTCTC AGGGAGAAAA GCAACTACAG CGACATCCCC 
GATGTCAAGA ACGACTTTGC CTTCATCCTC CACCTGGCCG ACCAGTACGA CCCCCTGTAC 
TCTAAGCGCT TCTCCATCTT CCTGTCGGAG GTCAGCGAGA ACAAACTGAA ACAGATCAAC 
CTCAACAACG AGTGGACCGT GGAGAGACTG AAGAGCAAGC TCGTGAAAAA CTCGCAGGAC 
AAGGTCGAGC TGCATCTCTT CATGCTCAAC GGTCTCCCGG ATAACGTCTT TGAGCTGACG 
GAGATGGAAG TGCTGAGCCT GGAGCTGATC CCGGAAGTCA AGCTGCCGGC TGCCGTCGCT 
CAGCTCGTCA ACCTCAGGGA GCTCCACGTG TACCATTCGT CCCTGGTGGT CGACCACCCC 
GCCCTGGCCT TTCTAGAGGA GAACTTAAGA ATCCTCCGCC TGAAATTTAC CGAGATGGGG 
AAGATCCCAC GCTGGGTGTT CCACCTGAAG AACCTCAAGG AGCTGTACCT GTCGGGCTGC 
GTTCTCCCGG AGCAGCTGAG CAGCTTGCAC CTGGAGGGCT TCCAGGACTT GAAGAACCTG 
AGGACCCTCT ACTTGAAGAG CAGCCTGTCG CGGATCCCTC AGGTGGTGAC AGACCTGCTG 
CCCTCCCTGC AGAAGCTGTC CCTTGATAAT GAGGGAAGCA AACTGGTTGT GCTGAACAAC 
CTGAAGAAGA TGGTCAATCT GAAAAGCCTG GAGCTGCTCA GCTGCGACCT GGAGCGCATC 
CCCCACTCCA TTTTCAGCCT GAACAACCTG CACGAGCTGG ACCTGAAGGA GAACAACCTC 
AAGACCGTAG AGGAGATCAT CAGCTTCCAG CACCTCCCGA GCCTCTCCTG CCTGAAGCTC 
TGGCACAATA ACATCGCTTA CATCCCCGCG CAGATCGGAG CGCTGTCTAA CCTGGAGCAG 
CTCTTCCTGG GTCACAACAA CATCGAGAGT CTCCCTCTGC AGCTTTTTCT ATGCACCAAA 
CTACACTACT TGGATCTAAG CTATAACCAT CTGACCTTCA TTCCCGAGGA AATCCAGTAC 
CTGACCAATC TGCAGTACTT TGCTGTGACC AACAATAACA TTGAGATGCT ACCAGACGGG 
CTGTTCCAAT GCAAGAAGCT TCAGTGTCTA CTCCTGGGAA GAAATAGCCT AACAGATTTG 
TCCCCTCTGG TGGGGGAGCT GTCAAACCTC ACCCACCTGG AACTCACTGG TAACTACCTG 
GAGACACTGC CTGTGGAGCT GGAAGGGTGT CAGTCTCTGA AACGGAGCTG TCTGATTGTC 
GAGGACAGTT TGCTCAATTC CCTCCCGCTT CCCGTGGCAG AACGCTTGCA GACCTGCTTA 
GATAAATGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030110511.1
CDS	445..2856
Translation	MITLTELKCLADAQSSYHILKPWWDVFWYYITLIMLLVAVLAGALQLTQSRVLCCLPCKVEFDNQCAVPWDLLKGSENASSNSGLLLPLPLRIQNDLHRQQYSYIDAVCYEKQLHWFAKFFPYLVLLHTLIFAACSNFWLHYPSTSSRLEHFVSILHKCFDSPWTTRALSETVAEQSVRPLKLSKSKTLLSTSGGSADIDASKQSLPYPQPGLESPGIESPTSSVLDKKEGEQAKAIFEKVKRFRLHVEQRDIIYRVYLKQIIVKVILFVLIITYVPYFLSYITLEIDCSIDVQAFTGYKRYQCVYSLAEIFKVLASFYVILVMLYGLTSSYSLWWMLRSSLKQYSFEALREKSNYSDIPDVKNDFAFILHLADQYDPLYSKRFSIFLSEVSENKLKQINLNNEWTVERLKSKLVKNSQDKVELHLFMLNGLPDNVFELTEMEVLSLELIPEVKLPAAVAQLVNLRELHVYHSSLVVDHPALAFLEENLRILRLKFTEMGKIPRWVFHLKNLKELYLSGCVLPEQLSSLHLEGFQDLKNLRTLYLKSSLSRIPQVVTDLLPSLQKLSLDNEGSKLVVLNNLKKMVNLKSLELLSCDLERIPHSIFSLNNLHELDLKENNLKTVEEIISFQHLPSLSCLKLWHNNIAYIPAQIGALSNLEQLFLGHNNIESLPLQLFLCTKLHYLDLSYNHLTFIPEEIQYLTNLQYFAVTNNNIEMLPDGLFQCKKLQCLLLGRNSLTDLSPLVGELSNLTHLELTGNYLETLPVELEGCQSLKRSCLIVEDSLLNSLPLPVAERLQTCLDKC

Target ORF information:

RefSeq Version	XM_030254651.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus leucine rich repeat containing 8 family, member B (Lrrc8b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030254651.1

ORF Insert Sequence:

ATGATTACAC TAACAGAGTT AAAATGCTTA GCGGACGCCC AGTCATCCTA CCACATACTG 
AAGCCGTGGT GGGATGTCTT CTGGTATTAC ATCACCCTCA TCATGCTGTT GGTGGCTGTG 
CTGGCCGGAG CCCTGCAGCT CACCCAGAGC AGGGTTCTCT GCTGTCTTCC CTGCAAGGTG 
GAATTTGACA ATCAGTGCGC TGTGCCTTGG GACCTCCTGA AAGGCAGCGA GAACGCATCT 
TCTAACTCGG GCCTGCTGCT CCCGCTGCCA CTCCGGATCC AGAACGACCT CCACCGGCAG 
CAGTACTCCT ACATCGATGC TGTCTGCTAC GAGAAACAGC TCCATTGGTT CGCCAAGTTT 
TTCCCCTATC TGGTGCTTCT GCACACCCTC ATCTTCGCAG CCTGCAGCAA CTTCTGGCTT 
CACTACCCCA GCACCAGCTC GAGGCTGGAA CACTTTGTGT CCATCCTCCA CAAGTGCTTC 
GACTCTCCGT GGACCACACG CGCCCTGTCA GAGACAGTGG CGGAACAGTC AGTGAGGCCC 
CTGAAGCTCT CCAAGTCCAA AACCTTGCTC TCAACCTCAG GGGGCTCCGC CGACATCGAC 
GCCAGCAAGC AGTCATTGCC CTACCCACAG CCAGGCTTGG AGTCGCCTGG CATAGAAAGC 
CCAACTTCTA GCGTCCTGGA CAAGAAGGAG GGCGAACAAG CCAAGGCCAT CTTTGAAAAA 
GTGAAGAGGT TCCGCCTGCA CGTGGAGCAG AGGGATATCA TCTACCGGGT GTACCTGAAG 
CAGATAATCG TTAAAGTCAT CCTGTTTGTC CTTATTATAA CCTACGTCCC CTACTTTCTC 
AGCTACATAA CTCTTGAAAT CGACTGTTCG ATCGACGTGC AGGCATTTAC AGGCTATAAG 
CGCTACCAGT GTGTCTACTC CTTGGCAGAA ATCTTCAAGG TCCTAGCTTC CTTTTACGTC 
ATTCTGGTGA TGCTTTACGG CCTCACGTCC TCCTACAGCC TGTGGTGGAT GCTGAGGAGT 
TCCTTGAAGC AGTACTCCTT TGAGGCTCTC AGGGAGAAAA GCAACTACAG CGACATCCCC 
GATGTCAAGA ACGACTTTGC CTTCATCCTC CACCTGGCCG ACCAGTACGA CCCCCTGTAC 
TCTAAGCGCT TCTCCATCTT CCTGTCGGAG GTCAGCGAGA ACAAACTGAA ACAGATCAAC 
CTCAACAACG AGTGGACCGT GGAGAGACTG AAGAGCAAGC TCGTGAAAAA CTCGCAGGAC 
AAGGTCGAGC TGCATCTCTT CATGCTCAAC GGTCTCCCGG ATAACGTCTT TGAGCTGACG 
GAGATGGAAG TGCTGAGCCT GGAGCTGATC CCGGAAGTCA AGCTGCCGGC TGCCGTCGCT 
CAGCTCGTCA ACCTCAGGGA GCTCCACGTG TACCATTCGT CCCTGGTGGT CGACCACCCC 
GCCCTGGCCT TTCTAGAGGA GAACTTAAGA ATCCTCCGCC TGAAATTTAC CGAGATGGGG 
AAGATCCCAC GCTGGGTGTT CCACCTGAAG AACCTCAAGG AGCTGTACCT GTCGGGCTGC 
GTTCTCCCGG AGCAGCTGAG CAGCTTGCAC CTGGAGGGCT TCCAGGACTT GAAGAACCTG 
AGGACCCTCT ACTTGAAGAG CAGCCTGTCG CGGATCCCTC AGGTGGTGAC AGACCTGCTG 
CCCTCCCTGC AGAAGCTGTC CCTTGATAAT GAGGGAAGCA AACTGGTTGT GCTGAACAAC 
CTGAAGAAGA TGGTCAATCT GAAAAGCCTG GAGCTGCTCA GCTGCGACCT GGAGCGCATC 
CCCCACTCCA TTTTCAGCCT GAACAACCTG CACGAGCTGG ACCTGAAGGA GAACAACCTC 
AAGACCGTAG AGGAGATCAT CAGCTTCCAG CACCTCCCGA GCCTCTCCTG CCTGAAGCTC 
TGGCACAATA ACATCGCTTA CATCCCCGCG CAGATCGGAG CGCTGTCTAA CCTGGAGCAG 
CTCTTCCTGG GTCACAACAA CATCGAGAGT CTCCCTCTGC AGCTTTTTCT ATGCACCAAA 
CTACACTACT TGGATCTAAG CTATAACCAT CTGACCTTCA TTCCCGAGGA AATCCAGTAC 
CTGACCAATC TGCAGTACTT TGCTGTGACC AACAATAACA TTGAGATGCT ACCAGACGGG 
CTGTTCCAAT GCAAGAAGCT TCAGTGTCTA CTCCTGGGAA GAAATAGCCT AACAGATTTG 
TCCCCTCTGG TGGGGGAGCT GTCAAACCTC ACCCACCTGG AACTCACTGG TAACTACCTG 
GAGACACTGC CTGTGGAGCT GGAAGGGTGT CAGTCTCTGA AACGGAGCTG TCTGATTGTC 
GAGGACAGTT TGCTCAATTC CCTCCCGCTT CCCGTGGCAG AACGCTTGCA GACCTGCTTA 
GATAAATGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lrrc8b ( XM_030254653.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030110513.1 Mus musculus leucine rich repeat containing 8 family, member B (Lrrc8b), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu19951
Clone ID Related Accession (Same CDS sequence)	XM_030254651.1 , XM_030254653.1 , XM_011249508.2 , NM_001033550.3 , XM_030254652.1 , NM_001033550.2 , XM_011249508.3
Accession Version	XM_030254653.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2412bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	volume-regulated anion channel subunit LRRC8B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTACAC TAACAGAGTT AAAATGCTTA GCGGACGCCC AGTCATCCTA CCACATACTG 
AAGCCGTGGT GGGATGTCTT CTGGTATTAC ATCACCCTCA TCATGCTGTT GGTGGCTGTG 
CTGGCCGGAG CCCTGCAGCT CACCCAGAGC AGGGTTCTCT GCTGTCTTCC CTGCAAGGTG 
GAATTTGACA ATCAGTGCGC TGTGCCTTGG GACCTCCTGA AAGGCAGCGA GAACGCATCT 
TCTAACTCGG GCCTGCTGCT CCCGCTGCCA CTCCGGATCC AGAACGACCT CCACCGGCAG 
CAGTACTCCT ACATCGATGC TGTCTGCTAC GAGAAACAGC TCCATTGGTT CGCCAAGTTT 
TTCCCCTATC TGGTGCTTCT GCACACCCTC ATCTTCGCAG CCTGCAGCAA CTTCTGGCTT 
CACTACCCCA GCACCAGCTC GAGGCTGGAA CACTTTGTGT CCATCCTCCA CAAGTGCTTC 
GACTCTCCGT GGACCACACG CGCCCTGTCA GAGACAGTGG CGGAACAGTC AGTGAGGCCC 
CTGAAGCTCT CCAAGTCCAA AACCTTGCTC TCAACCTCAG GGGGCTCCGC CGACATCGAC 
GCCAGCAAGC AGTCATTGCC CTACCCACAG CCAGGCTTGG AGTCGCCTGG CATAGAAAGC 
CCAACTTCTA GCGTCCTGGA CAAGAAGGAG GGCGAACAAG CCAAGGCCAT CTTTGAAAAA 
GTGAAGAGGT TCCGCCTGCA CGTGGAGCAG AGGGATATCA TCTACCGGGT GTACCTGAAG 
CAGATAATCG TTAAAGTCAT CCTGTTTGTC CTTATTATAA CCTACGTCCC CTACTTTCTC 
AGCTACATAA CTCTTGAAAT CGACTGTTCG ATCGACGTGC AGGCATTTAC AGGCTATAAG 
CGCTACCAGT GTGTCTACTC CTTGGCAGAA ATCTTCAAGG TCCTAGCTTC CTTTTACGTC 
ATTCTGGTGA TGCTTTACGG CCTCACGTCC TCCTACAGCC TGTGGTGGAT GCTGAGGAGT 
TCCTTGAAGC AGTACTCCTT TGAGGCTCTC AGGGAGAAAA GCAACTACAG CGACATCCCC 
GATGTCAAGA ACGACTTTGC CTTCATCCTC CACCTGGCCG ACCAGTACGA CCCCCTGTAC 
TCTAAGCGCT TCTCCATCTT CCTGTCGGAG GTCAGCGAGA ACAAACTGAA ACAGATCAAC 
CTCAACAACG AGTGGACCGT GGAGAGACTG AAGAGCAAGC TCGTGAAAAA CTCGCAGGAC 
AAGGTCGAGC TGCATCTCTT CATGCTCAAC GGTCTCCCGG ATAACGTCTT TGAGCTGACG 
GAGATGGAAG TGCTGAGCCT GGAGCTGATC CCGGAAGTCA AGCTGCCGGC TGCCGTCGCT 
CAGCTCGTCA ACCTCAGGGA GCTCCACGTG TACCATTCGT CCCTGGTGGT CGACCACCCC 
GCCCTGGCCT TTCTAGAGGA GAACTTAAGA ATCCTCCGCC TGAAATTTAC CGAGATGGGG 
AAGATCCCAC GCTGGGTGTT CCACCTGAAG AACCTCAAGG AGCTGTACCT GTCGGGCTGC 
GTTCTCCCGG AGCAGCTGAG CAGCTTGCAC CTGGAGGGCT TCCAGGACTT GAAGAACCTG 
AGGACCCTCT ACTTGAAGAG CAGCCTGTCG CGGATCCCTC AGGTGGTGAC AGACCTGCTG 
CCCTCCCTGC AGAAGCTGTC CCTTGATAAT GAGGGAAGCA AACTGGTTGT GCTGAACAAC 
CTGAAGAAGA TGGTCAATCT GAAAAGCCTG GAGCTGCTCA GCTGCGACCT GGAGCGCATC 
CCCCACTCCA TTTTCAGCCT GAACAACCTG CACGAGCTGG ACCTGAAGGA GAACAACCTC 
AAGACCGTAG AGGAGATCAT CAGCTTCCAG CACCTCCCGA GCCTCTCCTG CCTGAAGCTC 
TGGCACAATA ACATCGCTTA CATCCCCGCG CAGATCGGAG CGCTGTCTAA CCTGGAGCAG 
CTCTTCCTGG GTCACAACAA CATCGAGAGT CTCCCTCTGC AGCTTTTTCT ATGCACCAAA 
CTACACTACT TGGATCTAAG CTATAACCAT CTGACCTTCA TTCCCGAGGA AATCCAGTAC 
CTGACCAATC TGCAGTACTT TGCTGTGACC AACAATAACA TTGAGATGCT ACCAGACGGG 
CTGTTCCAAT GCAAGAAGCT TCAGTGTCTA CTCCTGGGAA GAAATAGCCT AACAGATTTG 
TCCCCTCTGG TGGGGGAGCT GTCAAACCTC ACCCACCTGG AACTCACTGG TAACTACCTG 
GAGACACTGC CTGTGGAGCT GGAAGGGTGT CAGTCTCTGA AACGGAGCTG TCTGATTGTC 
GAGGACAGTT TGCTCAATTC CCTCCCGCTT CCCGTGGCAG AACGCTTGCA GACCTGCTTA 
GATAAATGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030110513.1
CDS	288..2699
Translation	MITLTELKCLADAQSSYHILKPWWDVFWYYITLIMLLVAVLAGALQLTQSRVLCCLPCKVEFDNQCAVPWDLLKGSENASSNSGLLLPLPLRIQNDLHRQQYSYIDAVCYEKQLHWFAKFFPYLVLLHTLIFAACSNFWLHYPSTSSRLEHFVSILHKCFDSPWTTRALSETVAEQSVRPLKLSKSKTLLSTSGGSADIDASKQSLPYPQPGLESPGIESPTSSVLDKKEGEQAKAIFEKVKRFRLHVEQRDIIYRVYLKQIIVKVILFVLIITYVPYFLSYITLEIDCSIDVQAFTGYKRYQCVYSLAEIFKVLASFYVILVMLYGLTSSYSLWWMLRSSLKQYSFEALREKSNYSDIPDVKNDFAFILHLADQYDPLYSKRFSIFLSEVSENKLKQINLNNEWTVERLKSKLVKNSQDKVELHLFMLNGLPDNVFELTEMEVLSLELIPEVKLPAAVAQLVNLRELHVYHSSLVVDHPALAFLEENLRILRLKFTEMGKIPRWVFHLKNLKELYLSGCVLPEQLSSLHLEGFQDLKNLRTLYLKSSLSRIPQVVTDLLPSLQKLSLDNEGSKLVVLNNLKKMVNLKSLELLSCDLERIPHSIFSLNNLHELDLKENNLKTVEEIISFQHLPSLSCLKLWHNNIAYIPAQIGALSNLEQLFLGHNNIESLPLQLFLCTKLHYLDLSYNHLTFIPEEIQYLTNLQYFAVTNNNIEMLPDGLFQCKKLQCLLLGRNSLTDLSPLVGELSNLTHLELTGNYLETLPVELEGCQSLKRSCLIVEDSLLNSLPLPVAERLQTCLDKC

Target ORF information:

RefSeq Version	XM_030254653.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus leucine rich repeat containing 8 family, member B (Lrrc8b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030254653.1

ORF Insert Sequence:

ATGATTACAC TAACAGAGTT AAAATGCTTA GCGGACGCCC AGTCATCCTA CCACATACTG 
AAGCCGTGGT GGGATGTCTT CTGGTATTAC ATCACCCTCA TCATGCTGTT GGTGGCTGTG 
CTGGCCGGAG CCCTGCAGCT CACCCAGAGC AGGGTTCTCT GCTGTCTTCC CTGCAAGGTG 
GAATTTGACA ATCAGTGCGC TGTGCCTTGG GACCTCCTGA AAGGCAGCGA GAACGCATCT 
TCTAACTCGG GCCTGCTGCT CCCGCTGCCA CTCCGGATCC AGAACGACCT CCACCGGCAG 
CAGTACTCCT ACATCGATGC TGTCTGCTAC GAGAAACAGC TCCATTGGTT CGCCAAGTTT 
TTCCCCTATC TGGTGCTTCT GCACACCCTC ATCTTCGCAG CCTGCAGCAA CTTCTGGCTT 
CACTACCCCA GCACCAGCTC GAGGCTGGAA CACTTTGTGT CCATCCTCCA CAAGTGCTTC 
GACTCTCCGT GGACCACACG CGCCCTGTCA GAGACAGTGG CGGAACAGTC AGTGAGGCCC 
CTGAAGCTCT CCAAGTCCAA AACCTTGCTC TCAACCTCAG GGGGCTCCGC CGACATCGAC 
GCCAGCAAGC AGTCATTGCC CTACCCACAG CCAGGCTTGG AGTCGCCTGG CATAGAAAGC 
CCAACTTCTA GCGTCCTGGA CAAGAAGGAG GGCGAACAAG CCAAGGCCAT CTTTGAAAAA 
GTGAAGAGGT TCCGCCTGCA CGTGGAGCAG AGGGATATCA TCTACCGGGT GTACCTGAAG 
CAGATAATCG TTAAAGTCAT CCTGTTTGTC CTTATTATAA CCTACGTCCC CTACTTTCTC 
AGCTACATAA CTCTTGAAAT CGACTGTTCG ATCGACGTGC AGGCATTTAC AGGCTATAAG 
CGCTACCAGT GTGTCTACTC CTTGGCAGAA ATCTTCAAGG TCCTAGCTTC CTTTTACGTC 
ATTCTGGTGA TGCTTTACGG CCTCACGTCC TCCTACAGCC TGTGGTGGAT GCTGAGGAGT 
TCCTTGAAGC AGTACTCCTT TGAGGCTCTC AGGGAGAAAA GCAACTACAG CGACATCCCC 
GATGTCAAGA ACGACTTTGC CTTCATCCTC CACCTGGCCG ACCAGTACGA CCCCCTGTAC 
TCTAAGCGCT TCTCCATCTT CCTGTCGGAG GTCAGCGAGA ACAAACTGAA ACAGATCAAC 
CTCAACAACG AGTGGACCGT GGAGAGACTG AAGAGCAAGC TCGTGAAAAA CTCGCAGGAC 
AAGGTCGAGC TGCATCTCTT CATGCTCAAC GGTCTCCCGG ATAACGTCTT TGAGCTGACG 
GAGATGGAAG TGCTGAGCCT GGAGCTGATC CCGGAAGTCA AGCTGCCGGC TGCCGTCGCT 
CAGCTCGTCA ACCTCAGGGA GCTCCACGTG TACCATTCGT CCCTGGTGGT CGACCACCCC 
GCCCTGGCCT TTCTAGAGGA GAACTTAAGA ATCCTCCGCC TGAAATTTAC CGAGATGGGG 
AAGATCCCAC GCTGGGTGTT CCACCTGAAG AACCTCAAGG AGCTGTACCT GTCGGGCTGC 
GTTCTCCCGG AGCAGCTGAG CAGCTTGCAC CTGGAGGGCT TCCAGGACTT GAAGAACCTG 
AGGACCCTCT ACTTGAAGAG CAGCCTGTCG CGGATCCCTC AGGTGGTGAC AGACCTGCTG 
CCCTCCCTGC AGAAGCTGTC CCTTGATAAT GAGGGAAGCA AACTGGTTGT GCTGAACAAC 
CTGAAGAAGA TGGTCAATCT GAAAAGCCTG GAGCTGCTCA GCTGCGACCT GGAGCGCATC 
CCCCACTCCA TTTTCAGCCT GAACAACCTG CACGAGCTGG ACCTGAAGGA GAACAACCTC 
AAGACCGTAG AGGAGATCAT CAGCTTCCAG CACCTCCCGA GCCTCTCCTG CCTGAAGCTC 
TGGCACAATA ACATCGCTTA CATCCCCGCG CAGATCGGAG CGCTGTCTAA CCTGGAGCAG 
CTCTTCCTGG GTCACAACAA CATCGAGAGT CTCCCTCTGC AGCTTTTTCT ATGCACCAAA 
CTACACTACT TGGATCTAAG CTATAACCAT CTGACCTTCA TTCCCGAGGA AATCCAGTAC 
CTGACCAATC TGCAGTACTT TGCTGTGACC AACAATAACA TTGAGATGCT ACCAGACGGG 
CTGTTCCAAT GCAAGAAGCT TCAGTGTCTA CTCCTGGGAA GAAATAGCCT AACAGATTTG 
TCCCCTCTGG TGGGGGAGCT GTCAAACCTC ACCCACCTGG AACTCACTGG TAACTACCTG 
GAGACACTGC CTGTGGAGCT GGAAGGGTGT CAGTCTCTGA AACGGAGCTG TCTGATTGTC 
GAGGACAGTT TGCTCAATTC CCTCCCGCTT CCCGTGGCAG AACGCTTGCA GACCTGCTTA 
GATAAATGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lrrc8b ( XM_030254652.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030110512.1 Mus musculus leucine rich repeat containing 8 family, member B (Lrrc8b), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu19951
Clone ID Related Accession (Same CDS sequence)	XM_030254651.1 , XM_030254653.1 , XM_011249508.2 , NM_001033550.3 , XM_030254652.1 , NM_001033550.2 , XM_011249508.3
Accession Version	XM_030254652.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2412bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	volume-regulated anion channel subunit LRRC8B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTACAC TAACAGAGTT AAAATGCTTA GCGGACGCCC AGTCATCCTA CCACATACTG 
AAGCCGTGGT GGGATGTCTT CTGGTATTAC ATCACCCTCA TCATGCTGTT GGTGGCTGTG 
CTGGCCGGAG CCCTGCAGCT CACCCAGAGC AGGGTTCTCT GCTGTCTTCC CTGCAAGGTG 
GAATTTGACA ATCAGTGCGC TGTGCCTTGG GACCTCCTGA AAGGCAGCGA GAACGCATCT 
TCTAACTCGG GCCTGCTGCT CCCGCTGCCA CTCCGGATCC AGAACGACCT CCACCGGCAG 
CAGTACTCCT ACATCGATGC TGTCTGCTAC GAGAAACAGC TCCATTGGTT CGCCAAGTTT 
TTCCCCTATC TGGTGCTTCT GCACACCCTC ATCTTCGCAG CCTGCAGCAA CTTCTGGCTT 
CACTACCCCA GCACCAGCTC GAGGCTGGAA CACTTTGTGT CCATCCTCCA CAAGTGCTTC 
GACTCTCCGT GGACCACACG CGCCCTGTCA GAGACAGTGG CGGAACAGTC AGTGAGGCCC 
CTGAAGCTCT CCAAGTCCAA AACCTTGCTC TCAACCTCAG GGGGCTCCGC CGACATCGAC 
GCCAGCAAGC AGTCATTGCC CTACCCACAG CCAGGCTTGG AGTCGCCTGG CATAGAAAGC 
CCAACTTCTA GCGTCCTGGA CAAGAAGGAG GGCGAACAAG CCAAGGCCAT CTTTGAAAAA 
GTGAAGAGGT TCCGCCTGCA CGTGGAGCAG AGGGATATCA TCTACCGGGT GTACCTGAAG 
CAGATAATCG TTAAAGTCAT CCTGTTTGTC CTTATTATAA CCTACGTCCC CTACTTTCTC 
AGCTACATAA CTCTTGAAAT CGACTGTTCG ATCGACGTGC AGGCATTTAC AGGCTATAAG 
CGCTACCAGT GTGTCTACTC CTTGGCAGAA ATCTTCAAGG TCCTAGCTTC CTTTTACGTC 
ATTCTGGTGA TGCTTTACGG CCTCACGTCC TCCTACAGCC TGTGGTGGAT GCTGAGGAGT 
TCCTTGAAGC AGTACTCCTT TGAGGCTCTC AGGGAGAAAA GCAACTACAG CGACATCCCC 
GATGTCAAGA ACGACTTTGC CTTCATCCTC CACCTGGCCG ACCAGTACGA CCCCCTGTAC 
TCTAAGCGCT TCTCCATCTT CCTGTCGGAG GTCAGCGAGA ACAAACTGAA ACAGATCAAC 
CTCAACAACG AGTGGACCGT GGAGAGACTG AAGAGCAAGC TCGTGAAAAA CTCGCAGGAC 
AAGGTCGAGC TGCATCTCTT CATGCTCAAC GGTCTCCCGG ATAACGTCTT TGAGCTGACG 
GAGATGGAAG TGCTGAGCCT GGAGCTGATC CCGGAAGTCA AGCTGCCGGC TGCCGTCGCT 
CAGCTCGTCA ACCTCAGGGA GCTCCACGTG TACCATTCGT CCCTGGTGGT CGACCACCCC 
GCCCTGGCCT TTCTAGAGGA GAACTTAAGA ATCCTCCGCC TGAAATTTAC CGAGATGGGG 
AAGATCCCAC GCTGGGTGTT CCACCTGAAG AACCTCAAGG AGCTGTACCT GTCGGGCTGC 
GTTCTCCCGG AGCAGCTGAG CAGCTTGCAC CTGGAGGGCT TCCAGGACTT GAAGAACCTG 
AGGACCCTCT ACTTGAAGAG CAGCCTGTCG CGGATCCCTC AGGTGGTGAC AGACCTGCTG 
CCCTCCCTGC AGAAGCTGTC CCTTGATAAT GAGGGAAGCA AACTGGTTGT GCTGAACAAC 
CTGAAGAAGA TGGTCAATCT GAAAAGCCTG GAGCTGCTCA GCTGCGACCT GGAGCGCATC 
CCCCACTCCA TTTTCAGCCT GAACAACCTG CACGAGCTGG ACCTGAAGGA GAACAACCTC 
AAGACCGTAG AGGAGATCAT CAGCTTCCAG CACCTCCCGA GCCTCTCCTG CCTGAAGCTC 
TGGCACAATA ACATCGCTTA CATCCCCGCG CAGATCGGAG CGCTGTCTAA CCTGGAGCAG 
CTCTTCCTGG GTCACAACAA CATCGAGAGT CTCCCTCTGC AGCTTTTTCT ATGCACCAAA 
CTACACTACT TGGATCTAAG CTATAACCAT CTGACCTTCA TTCCCGAGGA AATCCAGTAC 
CTGACCAATC TGCAGTACTT TGCTGTGACC AACAATAACA TTGAGATGCT ACCAGACGGG 
CTGTTCCAAT GCAAGAAGCT TCAGTGTCTA CTCCTGGGAA GAAATAGCCT AACAGATTTG 
TCCCCTCTGG TGGGGGAGCT GTCAAACCTC ACCCACCTGG AACTCACTGG TAACTACCTG 
GAGACACTGC CTGTGGAGCT GGAAGGGTGT CAGTCTCTGA AACGGAGCTG TCTGATTGTC 
GAGGACAGTT TGCTCAATTC CCTCCCGCTT CCCGTGGCAG AACGCTTGCA GACCTGCTTA 
GATAAATGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030110512.1
CDS	280..2691
Translation	MITLTELKCLADAQSSYHILKPWWDVFWYYITLIMLLVAVLAGALQLTQSRVLCCLPCKVEFDNQCAVPWDLLKGSENASSNSGLLLPLPLRIQNDLHRQQYSYIDAVCYEKQLHWFAKFFPYLVLLHTLIFAACSNFWLHYPSTSSRLEHFVSILHKCFDSPWTTRALSETVAEQSVRPLKLSKSKTLLSTSGGSADIDASKQSLPYPQPGLESPGIESPTSSVLDKKEGEQAKAIFEKVKRFRLHVEQRDIIYRVYLKQIIVKVILFVLIITYVPYFLSYITLEIDCSIDVQAFTGYKRYQCVYSLAEIFKVLASFYVILVMLYGLTSSYSLWWMLRSSLKQYSFEALREKSNYSDIPDVKNDFAFILHLADQYDPLYSKRFSIFLSEVSENKLKQINLNNEWTVERLKSKLVKNSQDKVELHLFMLNGLPDNVFELTEMEVLSLELIPEVKLPAAVAQLVNLRELHVYHSSLVVDHPALAFLEENLRILRLKFTEMGKIPRWVFHLKNLKELYLSGCVLPEQLSSLHLEGFQDLKNLRTLYLKSSLSRIPQVVTDLLPSLQKLSLDNEGSKLVVLNNLKKMVNLKSLELLSCDLERIPHSIFSLNNLHELDLKENNLKTVEEIISFQHLPSLSCLKLWHNNIAYIPAQIGALSNLEQLFLGHNNIESLPLQLFLCTKLHYLDLSYNHLTFIPEEIQYLTNLQYFAVTNNNIEMLPDGLFQCKKLQCLLLGRNSLTDLSPLVGELSNLTHLELTGNYLETLPVELEGCQSLKRSCLIVEDSLLNSLPLPVAERLQTCLDKC

Target ORF information:

RefSeq Version	XM_030254652.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus leucine rich repeat containing 8 family, member B (Lrrc8b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030254652.1

ORF Insert Sequence:

ATGATTACAC TAACAGAGTT AAAATGCTTA GCGGACGCCC AGTCATCCTA CCACATACTG 
AAGCCGTGGT GGGATGTCTT CTGGTATTAC ATCACCCTCA TCATGCTGTT GGTGGCTGTG 
CTGGCCGGAG CCCTGCAGCT CACCCAGAGC AGGGTTCTCT GCTGTCTTCC CTGCAAGGTG 
GAATTTGACA ATCAGTGCGC TGTGCCTTGG GACCTCCTGA AAGGCAGCGA GAACGCATCT 
TCTAACTCGG GCCTGCTGCT CCCGCTGCCA CTCCGGATCC AGAACGACCT CCACCGGCAG 
CAGTACTCCT ACATCGATGC TGTCTGCTAC GAGAAACAGC TCCATTGGTT CGCCAAGTTT 
TTCCCCTATC TGGTGCTTCT GCACACCCTC ATCTTCGCAG CCTGCAGCAA CTTCTGGCTT 
CACTACCCCA GCACCAGCTC GAGGCTGGAA CACTTTGTGT CCATCCTCCA CAAGTGCTTC 
GACTCTCCGT GGACCACACG CGCCCTGTCA GAGACAGTGG CGGAACAGTC AGTGAGGCCC 
CTGAAGCTCT CCAAGTCCAA AACCTTGCTC TCAACCTCAG GGGGCTCCGC CGACATCGAC 
GCCAGCAAGC AGTCATTGCC CTACCCACAG CCAGGCTTGG AGTCGCCTGG CATAGAAAGC 
CCAACTTCTA GCGTCCTGGA CAAGAAGGAG GGCGAACAAG CCAAGGCCAT CTTTGAAAAA 
GTGAAGAGGT TCCGCCTGCA CGTGGAGCAG AGGGATATCA TCTACCGGGT GTACCTGAAG 
CAGATAATCG TTAAAGTCAT CCTGTTTGTC CTTATTATAA CCTACGTCCC CTACTTTCTC 
AGCTACATAA CTCTTGAAAT CGACTGTTCG ATCGACGTGC AGGCATTTAC AGGCTATAAG 
CGCTACCAGT GTGTCTACTC CTTGGCAGAA ATCTTCAAGG TCCTAGCTTC CTTTTACGTC 
ATTCTGGTGA TGCTTTACGG CCTCACGTCC TCCTACAGCC TGTGGTGGAT GCTGAGGAGT 
TCCTTGAAGC AGTACTCCTT TGAGGCTCTC AGGGAGAAAA GCAACTACAG CGACATCCCC 
GATGTCAAGA ACGACTTTGC CTTCATCCTC CACCTGGCCG ACCAGTACGA CCCCCTGTAC 
TCTAAGCGCT TCTCCATCTT CCTGTCGGAG GTCAGCGAGA ACAAACTGAA ACAGATCAAC 
CTCAACAACG AGTGGACCGT GGAGAGACTG AAGAGCAAGC TCGTGAAAAA CTCGCAGGAC 
AAGGTCGAGC TGCATCTCTT CATGCTCAAC GGTCTCCCGG ATAACGTCTT TGAGCTGACG 
GAGATGGAAG TGCTGAGCCT GGAGCTGATC CCGGAAGTCA AGCTGCCGGC TGCCGTCGCT 
CAGCTCGTCA ACCTCAGGGA GCTCCACGTG TACCATTCGT CCCTGGTGGT CGACCACCCC 
GCCCTGGCCT TTCTAGAGGA GAACTTAAGA ATCCTCCGCC TGAAATTTAC CGAGATGGGG 
AAGATCCCAC GCTGGGTGTT CCACCTGAAG AACCTCAAGG AGCTGTACCT GTCGGGCTGC 
GTTCTCCCGG AGCAGCTGAG CAGCTTGCAC CTGGAGGGCT TCCAGGACTT GAAGAACCTG 
AGGACCCTCT ACTTGAAGAG CAGCCTGTCG CGGATCCCTC AGGTGGTGAC AGACCTGCTG 
CCCTCCCTGC AGAAGCTGTC CCTTGATAAT GAGGGAAGCA AACTGGTTGT GCTGAACAAC 
CTGAAGAAGA TGGTCAATCT GAAAAGCCTG GAGCTGCTCA GCTGCGACCT GGAGCGCATC 
CCCCACTCCA TTTTCAGCCT GAACAACCTG CACGAGCTGG ACCTGAAGGA GAACAACCTC 
AAGACCGTAG AGGAGATCAT CAGCTTCCAG CACCTCCCGA GCCTCTCCTG CCTGAAGCTC 
TGGCACAATA ACATCGCTTA CATCCCCGCG CAGATCGGAG CGCTGTCTAA CCTGGAGCAG 
CTCTTCCTGG GTCACAACAA CATCGAGAGT CTCCCTCTGC AGCTTTTTCT ATGCACCAAA 
CTACACTACT TGGATCTAAG CTATAACCAT CTGACCTTCA TTCCCGAGGA AATCCAGTAC 
CTGACCAATC TGCAGTACTT TGCTGTGACC AACAATAACA TTGAGATGCT ACCAGACGGG 
CTGTTCCAAT GCAAGAAGCT TCAGTGTCTA CTCCTGGGAA GAAATAGCCT AACAGATTTG 
TCCCCTCTGG TGGGGGAGCT GTCAAACCTC ACCCACCTGG AACTCACTGG TAACTACCTG 
GAGACACTGC CTGTGGAGCT GGAAGGGTGT CAGTCTCTGA AACGGAGCTG TCTGATTGTC 
GAGGACAGTT TGCTCAATTC CCTCCCGCTT CCCGTGGCAG AACGCTTGCA GACCTGCTTA 
GATAAATGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lrrc8b ( XM_011249508.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011247810.1 Mus musculus leucine rich repeat containing 8 family, member B (Lrrc8b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu19951
Clone ID Related Accession (Same CDS sequence)	XM_030254651.1 , XM_030254653.1 , XM_011249508.2 , NM_001033550.3 , XM_030254652.1 , NM_001033550.2 , XM_011249508.3
Accession Version	XM_011249508.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2412bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	volume-regulated anion channel subunit LRRC8B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011249508.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTACAC TAACAGAGTT AAAATGCTTA GCGGACGCCC AGTCATCCTA CCACATACTG 
AAGCCGTGGT GGGATGTCTT CTGGTATTAC ATCACCCTCA TCATGCTGTT GGTGGCTGTG 
CTGGCCGGAG CCCTGCAGCT CACCCAGAGC AGGGTTCTCT GCTGTCTTCC CTGCAAGGTG 
GAATTTGACA ATCAGTGCGC TGTGCCTTGG GACCTCCTGA AAGGCAGCGA GAACGCATCT 
TCTAACTCGG GCCTGCTGCT CCCGCTGCCA CTCCGGATCC AGAACGACCT CCACCGGCAG 
CAGTACTCCT ACATCGATGC TGTCTGCTAC GAGAAACAGC TCCATTGGTT CGCCAAGTTT 
TTCCCCTATC TGGTGCTTCT GCACACCCTC ATCTTCGCAG CCTGCAGCAA CTTCTGGCTT 
CACTACCCCA GCACCAGCTC GAGGCTGGAA CACTTTGTGT CCATCCTCCA CAAGTGCTTC 
GACTCTCCGT GGACCACACG CGCCCTGTCA GAGACAGTGG CGGAACAGTC AGTGAGGCCC 
CTGAAGCTCT CCAAGTCCAA AACCTTGCTC TCAACCTCAG GGGGCTCCGC CGACATCGAC 
GCCAGCAAGC AGTCATTGCC CTACCCACAG CCAGGCTTGG AGTCGCCTGG CATAGAAAGC 
CCAACTTCTA GCGTCCTGGA CAAGAAGGAG GGCGAACAAG CCAAGGCCAT CTTTGAAAAA 
GTGAAGAGGT TCCGCCTGCA CGTGGAGCAG AGGGATATCA TCTACCGGGT GTACCTGAAG 
CAGATAATCG TTAAAGTCAT CCTGTTTGTC CTTATTATAA CCTACGTCCC CTACTTTCTC 
AGCTACATAA CTCTTGAAAT CGACTGTTCG ATCGACGTGC AGGCATTTAC AGGCTATAAG 
CGCTACCAGT GTGTCTACTC CTTGGCAGAA ATCTTCAAGG TCCTAGCTTC CTTTTACGTC 
ATTCTGGTGA TGCTTTACGG CCTCACGTCC TCCTACAGCC TGTGGTGGAT GCTGAGGAGT 
TCCTTGAAGC AGTACTCCTT TGAGGCTCTC AGGGAGAAAA GCAACTACAG CGACATCCCC 
GATGTCAAGA ACGACTTTGC CTTCATCCTC CACCTGGCCG ACCAGTACGA CCCCCTGTAC 
TCTAAGCGCT TCTCCATCTT CCTGTCGGAG GTCAGCGAGA ACAAACTGAA ACAGATCAAC 
CTCAACAACG AGTGGACCGT GGAGAGACTG AAGAGCAAGC TCGTGAAAAA CTCGCAGGAC 
AAGGTCGAGC TGCATCTCTT CATGCTCAAC GGTCTCCCGG ATAACGTCTT TGAGCTGACG 
GAGATGGAAG TGCTGAGCCT GGAGCTGATC CCGGAAGTCA AGCTGCCGGC TGCCGTCGCT 
CAGCTCGTCA ACCTCAGGGA GCTCCACGTG TACCATTCGT CCCTGGTGGT CGACCACCCC 
GCCCTGGCCT TTCTAGAGGA GAACTTAAGA ATCCTCCGCC TGAAATTTAC CGAGATGGGG 
AAGATCCCAC GCTGGGTGTT CCACCTGAAG AACCTCAAGG AGCTGTACCT GTCGGGCTGC 
GTTCTCCCGG AGCAGCTGAG CAGCTTGCAC CTGGAGGGCT TCCAGGACTT GAAGAACCTG 
AGGACCCTCT ACTTGAAGAG CAGCCTGTCG CGGATCCCTC AGGTGGTGAC AGACCTGCTG 
CCCTCCCTGC AGAAGCTGTC CCTTGATAAT GAGGGAAGCA AACTGGTTGT GCTGAACAAC 
CTGAAGAAGA TGGTCAATCT GAAAAGCCTG GAGCTGCTCA GCTGCGACCT GGAGCGCATC 
CCCCACTCCA TTTTCAGCCT GAACAACCTG CACGAGCTGG ACCTGAAGGA GAACAACCTC 
AAGACCGTAG AGGAGATCAT CAGCTTCCAG CACCTCCCGA GCCTCTCCTG CCTGAAGCTC 
TGGCACAATA ACATCGCTTA CATCCCCGCG CAGATCGGAG CGCTGTCTAA CCTGGAGCAG 
CTCTTCCTGG GTCACAACAA CATCGAGAGT CTCCCTCTGC AGCTTTTTCT ATGCACCAAA 
CTACACTACT TGGATCTAAG CTATAACCAT CTGACCTTCA TTCCCGAGGA AATCCAGTAC 
CTGACCAATC TGCAGTACTT TGCTGTGACC AACAATAACA TTGAGATGCT ACCAGACGGG 
CTGTTCCAAT GCAAGAAGCT TCAGTGTCTA CTCCTGGGAA GAAATAGCCT AACAGATTTG 
TCCCCTCTGG TGGGGGAGCT GTCAAACCTC ACCCACCTGG AACTCACTGG TAACTACCTG 
GAGACACTGC CTGTGGAGCT GGAAGGGTGT CAGTCTCTGA AACGGAGCTG TCTGATTGTC 
GAGGACAGTT TGCTCAATTC CCTCCCGCTT CCCGTGGCAG AACGCTTGCA GACCTGCTTA 
GATAAATGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011247810.1
CDS	1046..3457
Translation	MITLTELKCLADAQSSYHILKPWWDVFWYYITLIMLLVAVLAGALQLTQSRVLCCLPCKVEFDNQCAVPWDLLKGSENASSNSGLLLPLPLRIQNDLHRQQYSYIDAVCYEKQLHWFAKFFPYLVLLHTLIFAACSNFWLHYPSTSSRLEHFVSILHKCFDSPWTTRALSETVAEQSVRPLKLSKSKTLLSTSGGSADIDASKQSLPYPQPGLESPGIESPTSSVLDKKEGEQAKAIFEKVKRFRLHVEQRDIIYRVYLKQIIVKVILFVLIITYVPYFLSYITLEIDCSIDVQAFTGYKRYQCVYSLAEIFKVLASFYVILVMLYGLTSSYSLWWMLRSSLKQYSFEALREKSNYSDIPDVKNDFAFILHLADQYDPLYSKRFSIFLSEVSENKLKQINLNNEWTVERLKSKLVKNSQDKVELHLFMLNGLPDNVFELTEMEVLSLELIPEVKLPAAVAQLVNLRELHVYHSSLVVDHPALAFLEENLRILRLKFTEMGKIPRWVFHLKNLKELYLSGCVLPEQLSSLHLEGFQDLKNLRTLYLKSSLSRIPQVVTDLLPSLQKLSLDNEGSKLVVLNNLKKMVNLKSLELLSCDLERIPHSIFSLNNLHELDLKENNLKTVEEIISFQHLPSLSCLKLWHNNIAYIPAQIGALSNLEQLFLGHNNIESLPLQLFLCTKLHYLDLSYNHLTFIPEEIQYLTNLQYFAVTNNNIEMLPDGLFQCKKLQCLLLGRNSLTDLSPLVGELSNLTHLELTGNYLETLPVELEGCQSLKRSCLIVEDSLLNSLPLPVAERLQTCLDKC

Target ORF information:

RefSeq Version	XM_011249508.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus leucine rich repeat containing 8 family, member B (Lrrc8b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011249508.3

ORF Insert Sequence:

ATGATTACAC TAACAGAGTT AAAATGCTTA GCGGACGCCC AGTCATCCTA CCACATACTG 
AAGCCGTGGT GGGATGTCTT CTGGTATTAC ATCACCCTCA TCATGCTGTT GGTGGCTGTG 
CTGGCCGGAG CCCTGCAGCT CACCCAGAGC AGGGTTCTCT GCTGTCTTCC CTGCAAGGTG 
GAATTTGACA ATCAGTGCGC TGTGCCTTGG GACCTCCTGA AAGGCAGCGA GAACGCATCT 
TCTAACTCGG GCCTGCTGCT CCCGCTGCCA CTCCGGATCC AGAACGACCT CCACCGGCAG 
CAGTACTCCT ACATCGATGC TGTCTGCTAC GAGAAACAGC TCCATTGGTT CGCCAAGTTT 
TTCCCCTATC TGGTGCTTCT GCACACCCTC ATCTTCGCAG CCTGCAGCAA CTTCTGGCTT 
CACTACCCCA GCACCAGCTC GAGGCTGGAA CACTTTGTGT CCATCCTCCA CAAGTGCTTC 
GACTCTCCGT GGACCACACG CGCCCTGTCA GAGACAGTGG CGGAACAGTC AGTGAGGCCC 
CTGAAGCTCT CCAAGTCCAA AACCTTGCTC TCAACCTCAG GGGGCTCCGC CGACATCGAC 
GCCAGCAAGC AGTCATTGCC CTACCCACAG CCAGGCTTGG AGTCGCCTGG CATAGAAAGC 
CCAACTTCTA GCGTCCTGGA CAAGAAGGAG GGCGAACAAG CCAAGGCCAT CTTTGAAAAA 
GTGAAGAGGT TCCGCCTGCA CGTGGAGCAG AGGGATATCA TCTACCGGGT GTACCTGAAG 
CAGATAATCG TTAAAGTCAT CCTGTTTGTC CTTATTATAA CCTACGTCCC CTACTTTCTC 
AGCTACATAA CTCTTGAAAT CGACTGTTCG ATCGACGTGC AGGCATTTAC AGGCTATAAG 
CGCTACCAGT GTGTCTACTC CTTGGCAGAA ATCTTCAAGG TCCTAGCTTC CTTTTACGTC 
ATTCTGGTGA TGCTTTACGG CCTCACGTCC TCCTACAGCC TGTGGTGGAT GCTGAGGAGT 
TCCTTGAAGC AGTACTCCTT TGAGGCTCTC AGGGAGAAAA GCAACTACAG CGACATCCCC 
GATGTCAAGA ACGACTTTGC CTTCATCCTC CACCTGGCCG ACCAGTACGA CCCCCTGTAC 
TCTAAGCGCT TCTCCATCTT CCTGTCGGAG GTCAGCGAGA ACAAACTGAA ACAGATCAAC 
CTCAACAACG AGTGGACCGT GGAGAGACTG AAGAGCAAGC TCGTGAAAAA CTCGCAGGAC 
AAGGTCGAGC TGCATCTCTT CATGCTCAAC GGTCTCCCGG ATAACGTCTT TGAGCTGACG 
GAGATGGAAG TGCTGAGCCT GGAGCTGATC CCGGAAGTCA AGCTGCCGGC TGCCGTCGCT 
CAGCTCGTCA ACCTCAGGGA GCTCCACGTG TACCATTCGT CCCTGGTGGT CGACCACCCC 
GCCCTGGCCT TTCTAGAGGA GAACTTAAGA ATCCTCCGCC TGAAATTTAC CGAGATGGGG 
AAGATCCCAC GCTGGGTGTT CCACCTGAAG AACCTCAAGG AGCTGTACCT GTCGGGCTGC 
GTTCTCCCGG AGCAGCTGAG CAGCTTGCAC CTGGAGGGCT TCCAGGACTT GAAGAACCTG 
AGGACCCTCT ACTTGAAGAG CAGCCTGTCG CGGATCCCTC AGGTGGTGAC AGACCTGCTG 
CCCTCCCTGC AGAAGCTGTC CCTTGATAAT GAGGGAAGCA AACTGGTTGT GCTGAACAAC 
CTGAAGAAGA TGGTCAATCT GAAAAGCCTG GAGCTGCTCA GCTGCGACCT GGAGCGCATC 
CCCCACTCCA TTTTCAGCCT GAACAACCTG CACGAGCTGG ACCTGAAGGA GAACAACCTC 
AAGACCGTAG AGGAGATCAT CAGCTTCCAG CACCTCCCGA GCCTCTCCTG CCTGAAGCTC 
TGGCACAATA ACATCGCTTA CATCCCCGCG CAGATCGGAG CGCTGTCTAA CCTGGAGCAG 
CTCTTCCTGG GTCACAACAA CATCGAGAGT CTCCCTCTGC AGCTTTTTCT ATGCACCAAA 
CTACACTACT TGGATCTAAG CTATAACCAT CTGACCTTCA TTCCCGAGGA AATCCAGTAC 
CTGACCAATC TGCAGTACTT TGCTGTGACC AACAATAACA TTGAGATGCT ACCAGACGGG 
CTGTTCCAAT GCAAGAAGCT TCAGTGTCTA CTCCTGGGAA GAAATAGCCT AACAGATTTG 
TCCCCTCTGG TGGGGGAGCT GTCAAACCTC ACCCACCTGG AACTCACTGG TAACTACCTG 
GAGACACTGC CTGTGGAGCT GGAAGGGTGT CAGTCTCTGA AACGGAGCTG TCTGATTGTC 
GAGGACAGTT TGCTCAATTC CCTCCCGCTT CCCGTGGCAG AACGCTTGCA GACCTGCTTA 
GATAAATGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium.
Briefings in bioinformatics12(5)449-62(2011 Sep)
Gaudet P,Livstone MS,Lewis SE,Thomas PD

A high-resolution anatomical atlas of the transcriptome in the mouse embryo.
PLoS biology9(1)e1000582(2011 Jan)
Diez-Roux G,Banfi S,Sultan M,Geffers L,Anand S,Rozado D,Magen A,Canidio E,Pagani M,Peluso I,Lin-Marq N,Koch M,Bilio M,Cantiello I,Verde R,De Masi C,Bianchi SA,Cicchini J,Perroud E,Mehmeti S,Dagand E,Schrinner S,N?rnberger A,Schmidt K,Metz K,Zwingmann C,Brieske N,Springer C,Hernandez AM,Herzog S,Grabbe F,Sieverding C,Fischer B,Schrader K,Brockmeyer M,Dettmer S,Helbig C,Alunni V,Battaini MA,Mura C,Henrichsen CN,Garcia-Lopez R,Echevarria D,Puelles E,Garcia-Calero E,Kruse S,Uhr M,Kauck C,Feng G,Milyaev N,Ong CK,Kumar L,Lam M,Semple CA,Gyenesei A,Mundlos S,Radelof U,Lehrach H,Sarmientos P,Reymond A,Davidson DR,Doll? P,Antonarakis SE,Yaspo ML,Martinez S,Baldock RA,Eichele G,Ballabio A

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Lrrc8b cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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