Acsl5 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Acsl5
Entrez Gene ID	433256
Full Name	acyl-CoA synthetase long-chain family member 5
Synonyms	1700030F05Rik,ACS2,ACS5,Facl5
General protein information	Preferred Names acyl-CoA synthetase long-chain family member 5 Names long-chain-fatty-acid--CoA ligase 5 LACS 5 fatty acid Coenzyme A ligase, long chain 5 long-chain acyl-CoA synthetase 5
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 19 Map Location: 19\|19 D2

mRNA and Protein(s)

mRNA	Protein	Name
NM_027976.2	NP_082252.1	long-chain-fatty-acid--CoA ligase 5
XM_006527178.1	XP_006527241.1	long-chain-fatty-acid--CoA ligase 5 isoform X1
XM_006527178.2	XP_006527241.1	long-chain-fatty-acid--CoA ligase 5 isoform X1
XM_006527178.3	XP_006527241.1	long-chain-fatty-acid--CoA ligase 5 isoform X1

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG3961	NP_730369.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011603	XP_320900.3
Arabidopsis thaliana (thale cress)	LACS6	NP_566265.1
Oryza sativa (rice)	Os11g0147000	NP_001065739.1
Oryza sativa (rice)	Os12g0143900	NP_001066139.1
Canis lupus familiaris (dog)	ACSL5	XP_005637786.1
Mus musculus (house mouse)	Acsl5	NP_082252.1
Homo sapiens (human)	ACSL5	NP_057318.2
Bos taurus (cattle)	ACSL5	NP_001069118.1
Danio rerio (zebrafish)	acsl5	NP_001004599.1
Caenorhabditis elegans (roundworm)	acs-13	NP_490744.1
Pan troglodytes (chimpanzee)	ACSL5	XP_001146649.1
Rattus norvegicus (Norway rat)	Acsl5	NP_446059.1
Gallus gallus (chicken)	ACSL5	NP_001026408.1
Xenopus tropicalis (tropical clawed frog)	acsl5	NP_001011069.1

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Acyl CoA synthetase 5 (ACSL5) ablation in mice increases energy expenditure and insulin sensitivity and delays fat absorption.
Bowman TA, O'Keeffe KR, D'Aquila T, Yan QW, Griffin JD, Killion EA, Salter DM, Mashek DG, Buhman KK, Greenberg AS
Molecular metabolism5(3)210-20(2016 Mar)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

Targeting of Acyl-CoA synthetase 5 decreases jejunal fatty acid activation with no effect on dietary long-chain fatty acid absorption.
Meller N, Morgan ME, Wong WP, Altemus JB, Sehayek E
Lipids in health and disease1288(2013 Jun)

The Sj?gren-Larsson syndrome gene encodes a hexadecenal dehydrogenase of the sphingosine 1-phosphate degradation pathway.
Nakahara K, Ohkuni A, Kitamura T, Abe K, Naganuma T, Ohno Y, Zoeller RA, Kihara A
Molecular cell46(4)461-71(2012 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Acsl5 is a major activator of dietary long chain fatty acids , yet in Acsl5 KO mice residual ACS activity is sufficient for maintaining a normal long chain fatty acids absorption.
Title: Targeting of Acyl-CoA synthetase 5 decreases jejunal fatty acid activation with no effect on dietary long-chain fatty acid absorption.

Acyl-CoA synthetase 2 has a role in fatty acid internalization and neurite outgrowth
Title: Acyl-CoA synthetase 2 overexpression enhances fatty acid internalization and neurite outgrowth.

The following Acsl5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Acsl5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu04882	NM_027976.2 Latest version!	Mus musculus acyl-CoA synthetase long-chain family member 5 (Acsl5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
OMu04882	XM_006527178.3 Latest version!	Mus musculus acyl-CoA synthetase long-chain family member 5 (Acsl5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OMu04882	XM_006527178.1	Mus musculus acyl-CoA synthetase long-chain family member 5 (Acsl5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
OMu04882	XM_006527178.2	Mus musculus acyl-CoA synthetase long-chain family member 5 (Acsl5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Acsl5 ( NM_027976.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_082252.1 Mus musculus acyl-CoA synthetase long-chain family member 5 (Acsl5), mRNA.

Price & Availability↑

CloneID	OMu04882
Clone ID Related Accession (Same CDS sequence)	NM_027976.2 , XM_006527178.1 , XM_006527178.2 , XM_006527178.3
Accession Version	NM_027976.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2052bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	long-chain-fatty-acid--CoA ligase 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC133100.5, BC031544.1 and AI838021.1. On Sep 21, 2005 this sequence version replaced NM_027976.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK034942.1, SRR1660813.226872.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849377 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTTTTTA TTTTTAACTT CTTGTTTTCC CCACTTCCAA CTCCGGCCCT GATCTGCCTC 
CTGACGTTTG GAACGGCCAT CTTCCTGTGG CTGATCAACA GACCTCAGCC AGTTTTACCT 
CTCATTGACT TGGACAACCA GTCTGTGGGG ATTGAGGGAG GAGCACGGAG GGGCGCTTTC 
CAGAAGAACA ATGACCTAAT CCTTTATTAC TTCTCAGACG CCAAGACGTT GTATGAAAAT 
TTCCAAAGAG GACTTGCTGT GTCTGACAAT GGGCCTTGCT TGGGATACAG AAAGCCAAAC 
CAGCCCTACA AATGGATCTC CTACAAACAG GTGTCTGATC GAGCAGAGTA CCTGGGCTCC 
TGTCTTTTGC ATAAAGGATA TAAGTCATCA CAGGACCAAT TTGTTGGCAT CTTTGCTCAA 
AATAGGCCAG AGTGGGTCAT CTCTGAGTTA GCCTGTTACA CATATTCCAT GGTAGCTGTC 
CCCCTGTATG ACACGCTGGG TACAGAAGCC ATCATCTTTG TCATCAACAG GGCTGATATC 
CCTGTGGTCA TCTGTGATAC ACCCCAAAAG GCAACAATGC TAGTAGAAAA TGTGGAAAAG 
GGCCTCACAC CAGGCCTGAA GACGATCATC CTCATGGACC CTTTTGATGA CGACCTGATG 
AAAAGAGGAG AAAAGTGTGG AGTTGAGATG TTATCCCTGC ATGATGCGGA GAATATAGGC 
AAAGAGAACT TCAAAAAACC AGTGCCTCCG AAGCCAGAGG ATCTGAGCGT CATCTGTTTC 
ACCAGTGGAA CTACAGGTGA CCCCAAAGGA GCTATGCTAA CCCATGAAAA TGTTGTTTCA 
AACATGGCTG CTTTCCTCAA ATTTCTGGAG CCTATCTTCC AGCCCACCTC TGATGATGTG 
ACCATATCCT ACCTTCCCTT GGCTCATATG TTTGAGAGGC TTGTCCAGGG TATCTTATTT 
TCCTGTGGAG GCAAAATTGG GTTCTTCCAA GGAGATATCC GGTTGCTACC TGATGACATG 
AAGGCTTTGA AACCCACAGT GTTTCCTACA GTGCCTCGAC TCCTTAACAG GGTCTATGAT 
AAGGTACAAA ATGAAGCCAA GACACCTTTG AAGAAGTTCT TGTTGAACTT GGCTATCATC 
AGTAAATTCA ATGAAGTGAA AAATGGCATC ATTCGGCGGG ACAGTTTGTG GGACAAGCTC 
GTGTTTTCAA AGATTCAAGG AAGCCTGGGG GGGAAGGTTC GCCTCATGAT CACTGGAGCC 
GCCCCCATCT CCACTCCAGT CTTGACATTC TTCAGGGCTG CAATGGGATG TTGGGTGTTT 
GAAGCTTATG GCCAAACAGA ATGCACAGGT GGATGTTCAA TTACATCACC TGGGGACTGG 
ACAGCAGGTC ATGTTGGGAC TCCAGTGGCT TGCAATTTTG TAAAGCTGGA AGATGTGGCT 
GACATGAACT ACTTTTCAGT AAACAACGAA GGCGAGATCT GCATCAAAGG TAACAATGTG 
TTCAAAGGCT ACCTAAAGGA CCCAGAGAAA ACACAGGAAG TACTGGACAA GGATGGATGG 
CTTCACACTG GGGACATTGG TCGCTGGCTT CCAAATGGAA CTCTGAAAAT TGTTGACCGG 
AAGAAGAATA TTTTCAAGTT GGCACAAGGA GAATACATTG CCCCAGAGAA GATTGAAAAT 
GTTTATTCCA GGAGCAGACC AGTATTGCAA GTTTTTGTCC ATGGGGAGAG CTTACGGTCA 
TTCCTGATCG GAGTGGTGGT TCCCGACCCA GATTCACTTC CCTCATTTGC AGCCAAGATC 
GGGGTAAAGG GATCATTTGA AGAACTGTGC AAAAACCAAT GTGTAAAGGA AGCCATTTTA 
GAAGACTTGC AGAAAATTGG GAAAGAGGGT GGCCTGAAAT CCTTTGAGCA GGTCAAAAGT 
ATCTTTGTGC ATCCAGAGCC CTTCACTATT GAAAACGGAC TTCTGACACC GACACTGAAA 
GCCAAACGAG TAGAGCTTGC CAAGTTCTTC CAGACACAAA TCAAGAGCCT CTATGAGAGC 
ATCGAGGAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_082252.1
CDS	828..2879
Translation	MLFIFNFLFSPLPTPALICLLTFGTAIFLWLINRPQPVLPLIDLDNQSVGIEGGARRGAFQKNNDLILYYFSDAKTLYENFQRGLAVSDNGPCLGYRKPNQPYKWISYKQVSDRAEYLGSCLLHKGYKSSQDQFVGIFAQNRPEWVISELACYTYSMVAVPLYDTLGTEAIIFVINRADIPVVICDTPQKATMLVENVEKGLTPGLKTIILMDPFDDDLMKRGEKCGVEMLSLHDAENIGKENFKKPVPPKPEDLSVICFTSGTTGDPKGAMLTHENVVSNMAAFLKFLEPIFQPTSDDVTISYLPLAHMFERLVQGILFSCGGKIGFFQGDIRLLPDDMKALKPTVFPTVPRLLNRVYDKVQNEAKTPLKKFLLNLAIISKFNEVKNGIIRRDSLWDKLVFSKIQGSLGGKVRLMITGAAPISTPVLTFFRAAMGCWVFEAYGQTECTGGCSITSPGDWTAGHVGTPVACNFVKLEDVADMNYFSVNNEGEICIKGNNVFKGYLKDPEKTQEVLDKDGWLHTGDIGRWLPNGTLKIVDRKKNIFKLAQGEYIAPEKIENVYSRSRPVLQVFVHGESLRSFLIGVVVPDPDSLPSFAAKIGVKGSFEELCKNQCVKEAILEDLQKIGKEGGLKSFEQVKSIFVHPEPFTIENGLLTPTLKAKRVELAKFFQTQIKSLYESIEE

Target ORF information:

RefSeq Version	NM_027976.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus acyl-CoA synthetase long-chain family member 5 (Acsl5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_027976.2

ORF Insert Sequence:

ATGCTTTTTA TTTTTAACTT CTTGTTTTCC CCACTTCCAA CTCCGGCCCT GATCTGCCTC 
CTGACGTTTG GAACGGCCAT CTTCCTGTGG CTGATCAACA GACCTCAGCC AGTTTTACCT 
CTCATTGACT TGGACAACCA GTCTGTGGGG ATTGAGGGAG GAGCACGGAG GGGCGCTTTC 
CAGAAGAACA ATGACCTAAT CCTTTATTAC TTCTCAGACG CCAAGACGTT GTATGAAAAT 
TTCCAAAGAG GACTTGCTGT GTCTGACAAT GGGCCTTGCT TGGGATACAG AAAGCCAAAC 
CAGCCCTACA AATGGATCTC CTACAAACAG GTGTCTGATC GAGCAGAGTA CCTGGGCTCC 
TGTCTTTTGC ATAAAGGATA TAAGTCATCA CAGGACCAAT TTGTTGGCAT CTTTGCTCAA 
AATAGGCCAG AGTGGGTCAT CTCTGAGTTA GCCTGTTACA CATATTCCAT GGTAGCTGTC 
CCCCTGTATG ACACGCTGGG TACAGAAGCC ATCATCTTTG TCATCAACAG GGCTGATATC 
CCTGTGGTCA TCTGTGATAC ACCCCAAAAG GCAACAATGC TAGTAGAAAA TGTGGAAAAG 
GGCCTCACAC CAGGCCTGAA GACGATCATC CTCATGGACC CTTTTGATGA CGACCTGATG 
AAAAGAGGAG AAAAGTGTGG AGTTGAGATG TTATCCCTGC ATGATGCGGA GAATATAGGC 
AAAGAGAACT TCAAAAAACC AGTGCCTCCG AAGCCAGAGG ATCTGAGCGT CATCTGTTTC 
ACCAGTGGAA CTACAGGTGA CCCCAAAGGA GCTATGCTAA CCCATGAAAA TGTTGTTTCA 
AACATGGCTG CTTTCCTCAA ATTTCTGGAG CCTATCTTCC AGCCCACCTC TGATGATGTG 
ACCATATCCT ACCTTCCCTT GGCTCATATG TTTGAGAGGC TTGTCCAGGG TATCTTATTT 
TCCTGTGGAG GCAAAATTGG GTTCTTCCAA GGAGATATCC GGTTGCTACC TGATGACATG 
AAGGCTTTGA AACCCACAGT GTTTCCTACA GTGCCTCGAC TCCTTAACAG GGTCTATGAT 
AAGGTACAAA ATGAAGCCAA GACACCTTTG AAGAAGTTCT TGTTGAACTT GGCTATCATC 
AGTAAATTCA ATGAAGTGAA AAATGGCATC ATTCGGCGGG ACAGTTTGTG GGACAAGCTC 
GTGTTTTCAA AGATTCAAGG AAGCCTGGGG GGGAAGGTTC GCCTCATGAT CACTGGAGCC 
GCCCCCATCT CCACTCCAGT CTTGACATTC TTCAGGGCTG CAATGGGATG TTGGGTGTTT 
GAAGCTTATG GCCAAACAGA ATGCACAGGT GGATGTTCAA TTACATCACC TGGGGACTGG 
ACAGCAGGTC ATGTTGGGAC TCCAGTGGCT TGCAATTTTG TAAAGCTGGA AGATGTGGCT 
GACATGAACT ACTTTTCAGT AAACAACGAA GGCGAGATCT GCATCAAAGG TAACAATGTG 
TTCAAAGGCT ACCTAAAGGA CCCAGAGAAA ACACAGGAAG TACTGGACAA GGATGGATGG 
CTTCACACTG GGGACATTGG TCGCTGGCTT CCAAATGGAA CTCTGAAAAT TGTTGACCGG 
AAGAAGAATA TTTTCAAGTT GGCACAAGGA GAATACATTG CCCCAGAGAA GATTGAAAAT 
GTTTATTCCA GGAGCAGACC AGTATTGCAA GTTTTTGTCC ATGGGGAGAG CTTACGGTCA 
TTCCTGATCG GAGTGGTGGT TCCCGACCCA GATTCACTTC CCTCATTTGC AGCCAAGATC 
GGGGTAAAGG GATCATTTGA AGAACTGTGC AAAAACCAAT GTGTAAAGGA AGCCATTTTA 
GAAGACTTGC AGAAAATTGG GAAAGAGGGT GGCCTGAAAT CCTTTGAGCA GGTCAAAAGT 
ATCTTTGTGC ATCCAGAGCC CTTCACTATT GAAAACGGAC TTCTGACACC GACACTGAAA 
GCCAAACGAG TAGAGCTTGC CAAGTTCTTC CAGACACAAA TCAAGAGCCT CTATGAGAGC 
ATCGAGGAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Acsl5 ( XM_006527178.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006527241.1 Mus musculus acyl-CoA synthetase long-chain family member 5 (Acsl5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu04882
Clone ID Related Accession (Same CDS sequence)	NM_027976.2 , XM_006527178.1 , XM_006527178.2 , XM_006527178.3
Accession Version	XM_006527178.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2052bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	long-chain-fatty-acid--CoA ligase 5 isoform X1
Comment

ATGCTTTTTA TTTTTAACTT CTTGTTTTCC CCACTTCCAA CTCCGGCCCT GATCTGCCTC 
CTGACGTTTG GAACGGCCAT CTTCCTGTGG CTGATCAACA GACCTCAGCC AGTTTTACCT 
CTCATTGACT TGGACAACCA GTCTGTGGGG ATTGAGGGAG GAGCACGGAG GGGCGCTTTC 
CAGAAGAACA ATGACCTAAT CCTTTATTAC TTCTCAGACG CCAAGACGTT GTATGAAAAT 
TTCCAAAGAG GACTTGCTGT GTCTGACAAT GGGCCTTGCT TGGGATACAG AAAGCCAAAC 
CAGCCCTACA AATGGATCTC CTACAAACAG GTGTCTGATC GAGCAGAGTA CCTGGGCTCC 
TGTCTTTTGC ATAAAGGATA TAAGTCATCA CAGGACCAAT TTGTTGGCAT CTTTGCTCAA 
AATAGGCCAG AGTGGGTCAT CTCTGAGTTA GCCTGTTACA CATATTCCAT GGTAGCTGTC 
CCCCTGTATG ACACGCTGGG TACAGAAGCC ATCATCTTTG TCATCAACAG GGCTGATATC 
CCTGTGGTCA TCTGTGATAC ACCCCAAAAG GCAACAATGC TAGTAGAAAA TGTGGAAAAG 
GGCCTCACAC CAGGCCTGAA GACGATCATC CTCATGGACC CTTTTGATGA CGACCTGATG 
AAAAGAGGAG AAAAGTGTGG AGTTGAGATG TTATCCCTGC ATGATGCGGA GAATATAGGC 
AAAGAGAACT TCAAAAAACC AGTGCCTCCG AAGCCAGAGG ATCTGAGCGT CATCTGTTTC 
ACCAGTGGAA CTACAGGTGA CCCCAAAGGA GCTATGCTAA CCCATGAAAA TGTTGTTTCA 
AACATGGCTG CTTTCCTCAA ATTTCTGGAG CCTATCTTCC AGCCCACCTC TGATGATGTG 
ACCATATCCT ACCTTCCCTT GGCTCATATG TTTGAGAGGC TTGTCCAGGG TATCTTATTT 
TCCTGTGGAG GCAAAATTGG GTTCTTCCAA GGAGATATCC GGTTGCTACC TGATGACATG 
AAGGCTTTGA AACCCACAGT GTTTCCTACA GTGCCTCGAC TCCTTAACAG GGTCTATGAT 
AAGGTACAAA ATGAAGCCAA GACACCTTTG AAGAAGTTCT TGTTGAACTT GGCTATCATC 
AGTAAATTCA ATGAAGTGAA AAATGGCATC ATTCGGCGGG ACAGTTTGTG GGACAAGCTC 
GTGTTTTCAA AGATTCAAGG AAGCCTGGGG GGGAAGGTTC GCCTCATGAT CACTGGAGCC 
GCCCCCATCT CCACTCCAGT CTTGACATTC TTCAGGGCTG CAATGGGATG TTGGGTGTTT 
GAAGCTTATG GCCAAACAGA ATGCACAGGT GGATGTTCAA TTACATCACC TGGGGACTGG 
ACAGCAGGTC ATGTTGGGAC TCCAGTGGCT TGCAATTTTG TAAAGCTGGA AGATGTGGCT 
GACATGAACT ACTTTTCAGT AAACAACGAA GGCGAGATCT GCATCAAAGG TAACAATGTG 
TTCAAAGGCT ACCTAAAGGA CCCAGAGAAA ACACAGGAAG TACTGGACAA GGATGGATGG 
CTTCACACTG GGGACATTGG TCGCTGGCTT CCAAATGGAA CTCTGAAAAT TGTTGACCGG 
AAGAAGAATA TTTTCAAGTT GGCACAAGGA GAATACATTG CCCCAGAGAA GATTGAAAAT 
GTTTATTCCA GGAGCAGACC AGTATTGCAA GTTTTTGTCC ATGGGGAGAG CTTACGGTCA 
TTCCTGATCG GAGTGGTGGT TCCCGACCCA GATTCACTTC CCTCATTTGC AGCCAAGATC 
GGGGTAAAGG GATCATTTGA AGAACTGTGC AAAAACCAAT GTGTAAAGGA AGCCATTTTA 
GAAGACTTGC AGAAAATTGG GAAAGAGGGT GGCCTGAAAT CCTTTGAGCA GGTCAAAAGT 
ATCTTTGTGC ATCCAGAGCC CTTCACTATT GAAAACGGAC TTCTGACACC GACACTGAAA 
GCCAAACGAG TAGAGCTTGC CAAGTTCTTC CAGACACAAA TCAAGAGCCT CTATGAGAGC 
ATCGAGGAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_006527241.1

Translation

MLFIFNFLFSPLPTPALICLLTFGTAIFLWLINRPQPVLPLIDLDNQSVGIEGGARRGAFQKNNDLILYYFSDAKTLYENFQRGLAVSDNGPCLGYRKPNQPYKWISYKQVSDRAEYLGSCLLHKGYKSSQDQFVGIFAQNRPEWVISELACYTYSMVAVPLYDTLGTEAIIFVINRADIPVVICDTPQKATMLVENVEKGLTPGLKTIILMDPFDDDLMKRGEKCGVEMLSLHDAENIGKENFKKPVPPKPEDLSVICFTSGTTGDPKGAMLTHENVVSNMAAFLKFLEPIFQPTSDDVTISYLPLAHMFERLVQGILFSCGGKIGFFQGDIRLLPDDMKALKPTVFPTVPRLLNRVYDKVQNEAKTPLKKFLLNLAIISKFNEVKNGIIRRDSLWDKLVFSKIQGSLGGKVRLMITGAAPISTPVLTFFRAAMGCWVFEAYGQTECTGGCSITSPGDWTAGHVGTPVACNFVKLEDVADMNYFSVNNEGEICIKGNNVFKGYLKDPEKTQEVLDKDGWLHTGDIGRWLPNGTLKIVDRKKNIFKLAQGEYIAPEKIENVYSRSRPVLQVFVHGESLRSFLIGVVVPDPDSLPSFAAKIGVKGSFEELCKNQCVKEAILEDLQKIGKEGGLKSFEQVKSIFVHPEPFTIENGLLTPTLKAKRVELAKFFQTQIKSLYESIEE

Target ORF information:

RefSeq Version	XM_006527178.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus acyl-CoA synthetase long-chain family member 5 (Acsl5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006527178.1

ORF Insert Sequence:

ATGCTTTTTA TTTTTAACTT CTTGTTTTCC CCACTTCCAA CTCCGGCCCT GATCTGCCTC 
CTGACGTTTG GAACGGCCAT CTTCCTGTGG CTGATCAACA GACCTCAGCC AGTTTTACCT 
CTCATTGACT TGGACAACCA GTCTGTGGGG ATTGAGGGAG GAGCACGGAG GGGCGCTTTC 
CAGAAGAACA ATGACCTAAT CCTTTATTAC TTCTCAGACG CCAAGACGTT GTATGAAAAT 
TTCCAAAGAG GACTTGCTGT GTCTGACAAT GGGCCTTGCT TGGGATACAG AAAGCCAAAC 
CAGCCCTACA AATGGATCTC CTACAAACAG GTGTCTGATC GAGCAGAGTA CCTGGGCTCC 
TGTCTTTTGC ATAAAGGATA TAAGTCATCA CAGGACCAAT TTGTTGGCAT CTTTGCTCAA 
AATAGGCCAG AGTGGGTCAT CTCTGAGTTA GCCTGTTACA CATATTCCAT GGTAGCTGTC 
CCCCTGTATG ACACGCTGGG TACAGAAGCC ATCATCTTTG TCATCAACAG GGCTGATATC 
CCTGTGGTCA TCTGTGATAC ACCCCAAAAG GCAACAATGC TAGTAGAAAA TGTGGAAAAG 
GGCCTCACAC CAGGCCTGAA GACGATCATC CTCATGGACC CTTTTGATGA CGACCTGATG 
AAAAGAGGAG AAAAGTGTGG AGTTGAGATG TTATCCCTGC ATGATGCGGA GAATATAGGC 
AAAGAGAACT TCAAAAAACC AGTGCCTCCG AAGCCAGAGG ATCTGAGCGT CATCTGTTTC 
ACCAGTGGAA CTACAGGTGA CCCCAAAGGA GCTATGCTAA CCCATGAAAA TGTTGTTTCA 
AACATGGCTG CTTTCCTCAA ATTTCTGGAG CCTATCTTCC AGCCCACCTC TGATGATGTG 
ACCATATCCT ACCTTCCCTT GGCTCATATG TTTGAGAGGC TTGTCCAGGG TATCTTATTT 
TCCTGTGGAG GCAAAATTGG GTTCTTCCAA GGAGATATCC GGTTGCTACC TGATGACATG 
AAGGCTTTGA AACCCACAGT GTTTCCTACA GTGCCTCGAC TCCTTAACAG GGTCTATGAT 
AAGGTACAAA ATGAAGCCAA GACACCTTTG AAGAAGTTCT TGTTGAACTT GGCTATCATC 
AGTAAATTCA ATGAAGTGAA AAATGGCATC ATTCGGCGGG ACAGTTTGTG GGACAAGCTC 
GTGTTTTCAA AGATTCAAGG AAGCCTGGGG GGGAAGGTTC GCCTCATGAT CACTGGAGCC 
GCCCCCATCT CCACTCCAGT CTTGACATTC TTCAGGGCTG CAATGGGATG TTGGGTGTTT 
GAAGCTTATG GCCAAACAGA ATGCACAGGT GGATGTTCAA TTACATCACC TGGGGACTGG 
ACAGCAGGTC ATGTTGGGAC TCCAGTGGCT TGCAATTTTG TAAAGCTGGA AGATGTGGCT 
GACATGAACT ACTTTTCAGT AAACAACGAA GGCGAGATCT GCATCAAAGG TAACAATGTG 
TTCAAAGGCT ACCTAAAGGA CCCAGAGAAA ACACAGGAAG TACTGGACAA GGATGGATGG 
CTTCACACTG GGGACATTGG TCGCTGGCTT CCAAATGGAA CTCTGAAAAT TGTTGACCGG 
AAGAAGAATA TTTTCAAGTT GGCACAAGGA GAATACATTG CCCCAGAGAA GATTGAAAAT 
GTTTATTCCA GGAGCAGACC AGTATTGCAA GTTTTTGTCC ATGGGGAGAG CTTACGGTCA 
TTCCTGATCG GAGTGGTGGT TCCCGACCCA GATTCACTTC CCTCATTTGC AGCCAAGATC 
GGGGTAAAGG GATCATTTGA AGAACTGTGC AAAAACCAAT GTGTAAAGGA AGCCATTTTA 
GAAGACTTGC AGAAAATTGG GAAAGAGGGT GGCCTGAAAT CCTTTGAGCA GGTCAAAAGT 
ATCTTTGTGC ATCCAGAGCC CTTCACTATT GAAAACGGAC TTCTGACACC GACACTGAAA 
GCCAAACGAG TAGAGCTTGC CAAGTTCTTC CAGACACAAA TCAAGAGCCT CTATGAGAGC 
ATCGAGGAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Acsl5 ( XM_006527178.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006527241.1 Mus musculus acyl-CoA synthetase long-chain family member 5 (Acsl5), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu04882
Clone ID Related Accession (Same CDS sequence)	NM_027976.2 , XM_006527178.1 , XM_006527178.2 , XM_006527178.3
Accession Version	XM_006527178.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2052bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	long-chain-fatty-acid--CoA ligase 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006527178.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTTTTA TTTTTAACTT CTTGTTTTCC CCACTTCCAA CTCCGGCCCT GATCTGCCTC 
CTGACGTTTG GAACGGCCAT CTTCCTGTGG CTGATCAACA GACCTCAGCC AGTTTTACCT 
CTCATTGACT TGGACAACCA GTCTGTGGGG ATTGAGGGAG GAGCACGGAG GGGCGCTTTC 
CAGAAGAACA ATGACCTAAT CCTTTATTAC TTCTCAGACG CCAAGACGTT GTATGAAAAT 
TTCCAAAGAG GACTTGCTGT GTCTGACAAT GGGCCTTGCT TGGGATACAG AAAGCCAAAC 
CAGCCCTACA AATGGATCTC CTACAAACAG GTGTCTGATC GAGCAGAGTA CCTGGGCTCC 
TGTCTTTTGC ATAAAGGATA TAAGTCATCA CAGGACCAAT TTGTTGGCAT CTTTGCTCAA 
AATAGGCCAG AGTGGGTCAT CTCTGAGTTA GCCTGTTACA CATATTCCAT GGTAGCTGTC 
CCCCTGTATG ACACGCTGGG TACAGAAGCC ATCATCTTTG TCATCAACAG GGCTGATATC 
CCTGTGGTCA TCTGTGATAC ACCCCAAAAG GCAACAATGC TAGTAGAAAA TGTGGAAAAG 
GGCCTCACAC CAGGCCTGAA GACGATCATC CTCATGGACC CTTTTGATGA CGACCTGATG 
AAAAGAGGAG AAAAGTGTGG AGTTGAGATG TTATCCCTGC ATGATGCGGA GAATATAGGC 
AAAGAGAACT TCAAAAAACC AGTGCCTCCG AAGCCAGAGG ATCTGAGCGT CATCTGTTTC 
ACCAGTGGAA CTACAGGTGA CCCCAAAGGA GCTATGCTAA CCCATGAAAA TGTTGTTTCA 
AACATGGCTG CTTTCCTCAA ATTTCTGGAG CCTATCTTCC AGCCCACCTC TGATGATGTG 
ACCATATCCT ACCTTCCCTT GGCTCATATG TTTGAGAGGC TTGTCCAGGG TATCTTATTT 
TCCTGTGGAG GCAAAATTGG GTTCTTCCAA GGAGATATCC GGTTGCTACC TGATGACATG 
AAGGCTTTGA AACCCACAGT GTTTCCTACA GTGCCTCGAC TCCTTAACAG GGTCTATGAT 
AAGGTACAAA ATGAAGCCAA GACACCTTTG AAGAAGTTCT TGTTGAACTT GGCTATCATC 
AGTAAATTCA ATGAAGTGAA AAATGGCATC ATTCGGCGGG ACAGTTTGTG GGACAAGCTC 
GTGTTTTCAA AGATTCAAGG AAGCCTGGGG GGGAAGGTTC GCCTCATGAT CACTGGAGCC 
GCCCCCATCT CCACTCCAGT CTTGACATTC TTCAGGGCTG CAATGGGATG TTGGGTGTTT 
GAAGCTTATG GCCAAACAGA ATGCACAGGT GGATGTTCAA TTACATCACC TGGGGACTGG 
ACAGCAGGTC ATGTTGGGAC TCCAGTGGCT TGCAATTTTG TAAAGCTGGA AGATGTGGCT 
GACATGAACT ACTTTTCAGT AAACAACGAA GGCGAGATCT GCATCAAAGG TAACAATGTG 
TTCAAAGGCT ACCTAAAGGA CCCAGAGAAA ACACAGGAAG TACTGGACAA GGATGGATGG 
CTTCACACTG GGGACATTGG TCGCTGGCTT CCAAATGGAA CTCTGAAAAT TGTTGACCGG 
AAGAAGAATA TTTTCAAGTT GGCACAAGGA GAATACATTG CCCCAGAGAA GATTGAAAAT 
GTTTATTCCA GGAGCAGACC AGTATTGCAA GTTTTTGTCC ATGGGGAGAG CTTACGGTCA 
TTCCTGATCG GAGTGGTGGT TCCCGACCCA GATTCACTTC CCTCATTTGC AGCCAAGATC 
GGGGTAAAGG GATCATTTGA AGAACTGTGC AAAAACCAAT GTGTAAAGGA AGCCATTTTA 
GAAGACTTGC AGAAAATTGG GAAAGAGGGT GGCCTGAAAT CCTTTGAGCA GGTCAAAAGT 
ATCTTTGTGC ATCCAGAGCC CTTCACTATT GAAAACGGAC TTCTGACACC GACACTGAAA 
GCCAAACGAG TAGAGCTTGC CAAGTTCTTC CAGACACAAA TCAAGAGCCT CTATGAGAGC 
ATCGAGGAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006527241.1
CDS	220..2271
Translation	MLFIFNFLFSPLPTPALICLLTFGTAIFLWLINRPQPVLPLIDLDNQSVGIEGGARRGAFQKNNDLILYYFSDAKTLYENFQRGLAVSDNGPCLGYRKPNQPYKWISYKQVSDRAEYLGSCLLHKGYKSSQDQFVGIFAQNRPEWVISELACYTYSMVAVPLYDTLGTEAIIFVINRADIPVVICDTPQKATMLVENVEKGLTPGLKTIILMDPFDDDLMKRGEKCGVEMLSLHDAENIGKENFKKPVPPKPEDLSVICFTSGTTGDPKGAMLTHENVVSNMAAFLKFLEPIFQPTSDDVTISYLPLAHMFERLVQGILFSCGGKIGFFQGDIRLLPDDMKALKPTVFPTVPRLLNRVYDKVQNEAKTPLKKFLLNLAIISKFNEVKNGIIRRDSLWDKLVFSKIQGSLGGKVRLMITGAAPISTPVLTFFRAAMGCWVFEAYGQTECTGGCSITSPGDWTAGHVGTPVACNFVKLEDVADMNYFSVNNEGEICIKGNNVFKGYLKDPEKTQEVLDKDGWLHTGDIGRWLPNGTLKIVDRKKNIFKLAQGEYIAPEKIENVYSRSRPVLQVFVHGESLRSFLIGVVVPDPDSLPSFAAKIGVKGSFEELCKNQCVKEAILEDLQKIGKEGGLKSFEQVKSIFVHPEPFTIENGLLTPTLKAKRVELAKFFQTQIKSLYESIEE

Target ORF information:

RefSeq Version	XM_006527178.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus acyl-CoA synthetase long-chain family member 5 (Acsl5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006527178.2

ORF Insert Sequence:

ATGCTTTTTA TTTTTAACTT CTTGTTTTCC CCACTTCCAA CTCCGGCCCT GATCTGCCTC 
CTGACGTTTG GAACGGCCAT CTTCCTGTGG CTGATCAACA GACCTCAGCC AGTTTTACCT 
CTCATTGACT TGGACAACCA GTCTGTGGGG ATTGAGGGAG GAGCACGGAG GGGCGCTTTC 
CAGAAGAACA ATGACCTAAT CCTTTATTAC TTCTCAGACG CCAAGACGTT GTATGAAAAT 
TTCCAAAGAG GACTTGCTGT GTCTGACAAT GGGCCTTGCT TGGGATACAG AAAGCCAAAC 
CAGCCCTACA AATGGATCTC CTACAAACAG GTGTCTGATC GAGCAGAGTA CCTGGGCTCC 
TGTCTTTTGC ATAAAGGATA TAAGTCATCA CAGGACCAAT TTGTTGGCAT CTTTGCTCAA 
AATAGGCCAG AGTGGGTCAT CTCTGAGTTA GCCTGTTACA CATATTCCAT GGTAGCTGTC 
CCCCTGTATG ACACGCTGGG TACAGAAGCC ATCATCTTTG TCATCAACAG GGCTGATATC 
CCTGTGGTCA TCTGTGATAC ACCCCAAAAG GCAACAATGC TAGTAGAAAA TGTGGAAAAG 
GGCCTCACAC CAGGCCTGAA GACGATCATC CTCATGGACC CTTTTGATGA CGACCTGATG 
AAAAGAGGAG AAAAGTGTGG AGTTGAGATG TTATCCCTGC ATGATGCGGA GAATATAGGC 
AAAGAGAACT TCAAAAAACC AGTGCCTCCG AAGCCAGAGG ATCTGAGCGT CATCTGTTTC 
ACCAGTGGAA CTACAGGTGA CCCCAAAGGA GCTATGCTAA CCCATGAAAA TGTTGTTTCA 
AACATGGCTG CTTTCCTCAA ATTTCTGGAG CCTATCTTCC AGCCCACCTC TGATGATGTG 
ACCATATCCT ACCTTCCCTT GGCTCATATG TTTGAGAGGC TTGTCCAGGG TATCTTATTT 
TCCTGTGGAG GCAAAATTGG GTTCTTCCAA GGAGATATCC GGTTGCTACC TGATGACATG 
AAGGCTTTGA AACCCACAGT GTTTCCTACA GTGCCTCGAC TCCTTAACAG GGTCTATGAT 
AAGGTACAAA ATGAAGCCAA GACACCTTTG AAGAAGTTCT TGTTGAACTT GGCTATCATC 
AGTAAATTCA ATGAAGTGAA AAATGGCATC ATTCGGCGGG ACAGTTTGTG GGACAAGCTC 
GTGTTTTCAA AGATTCAAGG AAGCCTGGGG GGGAAGGTTC GCCTCATGAT CACTGGAGCC 
GCCCCCATCT CCACTCCAGT CTTGACATTC TTCAGGGCTG CAATGGGATG TTGGGTGTTT 
GAAGCTTATG GCCAAACAGA ATGCACAGGT GGATGTTCAA TTACATCACC TGGGGACTGG 
ACAGCAGGTC ATGTTGGGAC TCCAGTGGCT TGCAATTTTG TAAAGCTGGA AGATGTGGCT 
GACATGAACT ACTTTTCAGT AAACAACGAA GGCGAGATCT GCATCAAAGG TAACAATGTG 
TTCAAAGGCT ACCTAAAGGA CCCAGAGAAA ACACAGGAAG TACTGGACAA GGATGGATGG 
CTTCACACTG GGGACATTGG TCGCTGGCTT CCAAATGGAA CTCTGAAAAT TGTTGACCGG 
AAGAAGAATA TTTTCAAGTT GGCACAAGGA GAATACATTG CCCCAGAGAA GATTGAAAAT 
GTTTATTCCA GGAGCAGACC AGTATTGCAA GTTTTTGTCC ATGGGGAGAG CTTACGGTCA 
TTCCTGATCG GAGTGGTGGT TCCCGACCCA GATTCACTTC CCTCATTTGC AGCCAAGATC 
GGGGTAAAGG GATCATTTGA AGAACTGTGC AAAAACCAAT GTGTAAAGGA AGCCATTTTA 
GAAGACTTGC AGAAAATTGG GAAAGAGGGT GGCCTGAAAT CCTTTGAGCA GGTCAAAAGT 
ATCTTTGTGC ATCCAGAGCC CTTCACTATT GAAAACGGAC TTCTGACACC GACACTGAAA 
GCCAAACGAG TAGAGCTTGC CAAGTTCTTC CAGACACAAA TCAAGAGCCT CTATGAGAGC 
ATCGAGGAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Acsl5 ( XM_006527178.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006527241.1 Mus musculus acyl-CoA synthetase long-chain family member 5 (Acsl5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu04882
Clone ID Related Accession (Same CDS sequence)	NM_027976.2 , XM_006527178.1 , XM_006527178.2 , XM_006527178.3
Accession Version	XM_006527178.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2052bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	long-chain-fatty-acid--CoA ligase 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006527178.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTTTTA TTTTTAACTT CTTGTTTTCC CCACTTCCAA CTCCGGCCCT GATCTGCCTC 
CTGACGTTTG GAACGGCCAT CTTCCTGTGG CTGATCAACA GACCTCAGCC AGTTTTACCT 
CTCATTGACT TGGACAACCA GTCTGTGGGG ATTGAGGGAG GAGCACGGAG GGGCGCTTTC 
CAGAAGAACA ATGACCTAAT CCTTTATTAC TTCTCAGACG CCAAGACGTT GTATGAAAAT 
TTCCAAAGAG GACTTGCTGT GTCTGACAAT GGGCCTTGCT TGGGATACAG AAAGCCAAAC 
CAGCCCTACA AATGGATCTC CTACAAACAG GTGTCTGATC GAGCAGAGTA CCTGGGCTCC 
TGTCTTTTGC ATAAAGGATA TAAGTCATCA CAGGACCAAT TTGTTGGCAT CTTTGCTCAA 
AATAGGCCAG AGTGGGTCAT CTCTGAGTTA GCCTGTTACA CATATTCCAT GGTAGCTGTC 
CCCCTGTATG ACACGCTGGG TACAGAAGCC ATCATCTTTG TCATCAACAG GGCTGATATC 
CCTGTGGTCA TCTGTGATAC ACCCCAAAAG GCAACAATGC TAGTAGAAAA TGTGGAAAAG 
GGCCTCACAC CAGGCCTGAA GACGATCATC CTCATGGACC CTTTTGATGA CGACCTGATG 
AAAAGAGGAG AAAAGTGTGG AGTTGAGATG TTATCCCTGC ATGATGCGGA GAATATAGGC 
AAAGAGAACT TCAAAAAACC AGTGCCTCCG AAGCCAGAGG ATCTGAGCGT CATCTGTTTC 
ACCAGTGGAA CTACAGGTGA CCCCAAAGGA GCTATGCTAA CCCATGAAAA TGTTGTTTCA 
AACATGGCTG CTTTCCTCAA ATTTCTGGAG CCTATCTTCC AGCCCACCTC TGATGATGTG 
ACCATATCCT ACCTTCCCTT GGCTCATATG TTTGAGAGGC TTGTCCAGGG TATCTTATTT 
TCCTGTGGAG GCAAAATTGG GTTCTTCCAA GGAGATATCC GGTTGCTACC TGATGACATG 
AAGGCTTTGA AACCCACAGT GTTTCCTACA GTGCCTCGAC TCCTTAACAG GGTCTATGAT 
AAGGTACAAA ATGAAGCCAA GACACCTTTG AAGAAGTTCT TGTTGAACTT GGCTATCATC 
AGTAAATTCA ATGAAGTGAA AAATGGCATC ATTCGGCGGG ACAGTTTGTG GGACAAGCTC 
GTGTTTTCAA AGATTCAAGG AAGCCTGGGG GGGAAGGTTC GCCTCATGAT CACTGGAGCC 
GCCCCCATCT CCACTCCAGT CTTGACATTC TTCAGGGCTG CAATGGGATG TTGGGTGTTT 
GAAGCTTATG GCCAAACAGA ATGCACAGGT GGATGTTCAA TTACATCACC TGGGGACTGG 
ACAGCAGGTC ATGTTGGGAC TCCAGTGGCT TGCAATTTTG TAAAGCTGGA AGATGTGGCT 
GACATGAACT ACTTTTCAGT AAACAACGAA GGCGAGATCT GCATCAAAGG TAACAATGTG 
TTCAAAGGCT ACCTAAAGGA CCCAGAGAAA ACACAGGAAG TACTGGACAA GGATGGATGG 
CTTCACACTG GGGACATTGG TCGCTGGCTT CCAAATGGAA CTCTGAAAAT TGTTGACCGG 
AAGAAGAATA TTTTCAAGTT GGCACAAGGA GAATACATTG CCCCAGAGAA GATTGAAAAT 
GTTTATTCCA GGAGCAGACC AGTATTGCAA GTTTTTGTCC ATGGGGAGAG CTTACGGTCA 
TTCCTGATCG GAGTGGTGGT TCCCGACCCA GATTCACTTC CCTCATTTGC AGCCAAGATC 
GGGGTAAAGG GATCATTTGA AGAACTGTGC AAAAACCAAT GTGTAAAGGA AGCCATTTTA 
GAAGACTTGC AGAAAATTGG GAAAGAGGGT GGCCTGAAAT CCTTTGAGCA GGTCAAAAGT 
ATCTTTGTGC ATCCAGAGCC CTTCACTATT GAAAACGGAC TTCTGACACC GACACTGAAA 
GCCAAACGAG TAGAGCTTGC CAAGTTCTTC CAGACACAAA TCAAGAGCCT CTATGAGAGC 
ATCGAGGAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006527241.1
CDS	246..2297
Translation	MLFIFNFLFSPLPTPALICLLTFGTAIFLWLINRPQPVLPLIDLDNQSVGIEGGARRGAFQKNNDLILYYFSDAKTLYENFQRGLAVSDNGPCLGYRKPNQPYKWISYKQVSDRAEYLGSCLLHKGYKSSQDQFVGIFAQNRPEWVISELACYTYSMVAVPLYDTLGTEAIIFVINRADIPVVICDTPQKATMLVENVEKGLTPGLKTIILMDPFDDDLMKRGEKCGVEMLSLHDAENIGKENFKKPVPPKPEDLSVICFTSGTTGDPKGAMLTHENVVSNMAAFLKFLEPIFQPTSDDVTISYLPLAHMFERLVQGILFSCGGKIGFFQGDIRLLPDDMKALKPTVFPTVPRLLNRVYDKVQNEAKTPLKKFLLNLAIISKFNEVKNGIIRRDSLWDKLVFSKIQGSLGGKVRLMITGAAPISTPVLTFFRAAMGCWVFEAYGQTECTGGCSITSPGDWTAGHVGTPVACNFVKLEDVADMNYFSVNNEGEICIKGNNVFKGYLKDPEKTQEVLDKDGWLHTGDIGRWLPNGTLKIVDRKKNIFKLAQGEYIAPEKIENVYSRSRPVLQVFVHGESLRSFLIGVVVPDPDSLPSFAAKIGVKGSFEELCKNQCVKEAILEDLQKIGKEGGLKSFEQVKSIFVHPEPFTIENGLLTPTLKAKRVELAKFFQTQIKSLYESIEE

Target ORF information:

RefSeq Version	XM_006527178.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus acyl-CoA synthetase long-chain family member 5 (Acsl5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006527178.3

ORF Insert Sequence:

ATGCTTTTTA TTTTTAACTT CTTGTTTTCC CCACTTCCAA CTCCGGCCCT GATCTGCCTC 
CTGACGTTTG GAACGGCCAT CTTCCTGTGG CTGATCAACA GACCTCAGCC AGTTTTACCT 
CTCATTGACT TGGACAACCA GTCTGTGGGG ATTGAGGGAG GAGCACGGAG GGGCGCTTTC 
CAGAAGAACA ATGACCTAAT CCTTTATTAC TTCTCAGACG CCAAGACGTT GTATGAAAAT 
TTCCAAAGAG GACTTGCTGT GTCTGACAAT GGGCCTTGCT TGGGATACAG AAAGCCAAAC 
CAGCCCTACA AATGGATCTC CTACAAACAG GTGTCTGATC GAGCAGAGTA CCTGGGCTCC 
TGTCTTTTGC ATAAAGGATA TAAGTCATCA CAGGACCAAT TTGTTGGCAT CTTTGCTCAA 
AATAGGCCAG AGTGGGTCAT CTCTGAGTTA GCCTGTTACA CATATTCCAT GGTAGCTGTC 
CCCCTGTATG ACACGCTGGG TACAGAAGCC ATCATCTTTG TCATCAACAG GGCTGATATC 
CCTGTGGTCA TCTGTGATAC ACCCCAAAAG GCAACAATGC TAGTAGAAAA TGTGGAAAAG 
GGCCTCACAC CAGGCCTGAA GACGATCATC CTCATGGACC CTTTTGATGA CGACCTGATG 
AAAAGAGGAG AAAAGTGTGG AGTTGAGATG TTATCCCTGC ATGATGCGGA GAATATAGGC 
AAAGAGAACT TCAAAAAACC AGTGCCTCCG AAGCCAGAGG ATCTGAGCGT CATCTGTTTC 
ACCAGTGGAA CTACAGGTGA CCCCAAAGGA GCTATGCTAA CCCATGAAAA TGTTGTTTCA 
AACATGGCTG CTTTCCTCAA ATTTCTGGAG CCTATCTTCC AGCCCACCTC TGATGATGTG 
ACCATATCCT ACCTTCCCTT GGCTCATATG TTTGAGAGGC TTGTCCAGGG TATCTTATTT 
TCCTGTGGAG GCAAAATTGG GTTCTTCCAA GGAGATATCC GGTTGCTACC TGATGACATG 
AAGGCTTTGA AACCCACAGT GTTTCCTACA GTGCCTCGAC TCCTTAACAG GGTCTATGAT 
AAGGTACAAA ATGAAGCCAA GACACCTTTG AAGAAGTTCT TGTTGAACTT GGCTATCATC 
AGTAAATTCA ATGAAGTGAA AAATGGCATC ATTCGGCGGG ACAGTTTGTG GGACAAGCTC 
GTGTTTTCAA AGATTCAAGG AAGCCTGGGG GGGAAGGTTC GCCTCATGAT CACTGGAGCC 
GCCCCCATCT CCACTCCAGT CTTGACATTC TTCAGGGCTG CAATGGGATG TTGGGTGTTT 
GAAGCTTATG GCCAAACAGA ATGCACAGGT GGATGTTCAA TTACATCACC TGGGGACTGG 
ACAGCAGGTC ATGTTGGGAC TCCAGTGGCT TGCAATTTTG TAAAGCTGGA AGATGTGGCT 
GACATGAACT ACTTTTCAGT AAACAACGAA GGCGAGATCT GCATCAAAGG TAACAATGTG 
TTCAAAGGCT ACCTAAAGGA CCCAGAGAAA ACACAGGAAG TACTGGACAA GGATGGATGG 
CTTCACACTG GGGACATTGG TCGCTGGCTT CCAAATGGAA CTCTGAAAAT TGTTGACCGG 
AAGAAGAATA TTTTCAAGTT GGCACAAGGA GAATACATTG CCCCAGAGAA GATTGAAAAT 
GTTTATTCCA GGAGCAGACC AGTATTGCAA GTTTTTGTCC ATGGGGAGAG CTTACGGTCA 
TTCCTGATCG GAGTGGTGGT TCCCGACCCA GATTCACTTC CCTCATTTGC AGCCAAGATC 
GGGGTAAAGG GATCATTTGA AGAACTGTGC AAAAACCAAT GTGTAAAGGA AGCCATTTTA 
GAAGACTTGC AGAAAATTGG GAAAGAGGGT GGCCTGAAAT CCTTTGAGCA GGTCAAAAGT 
ATCTTTGTGC ATCCAGAGCC CTTCACTATT GAAAACGGAC TTCTGACACC GACACTGAAA 
GCCAAACGAG TAGAGCTTGC CAAGTTCTTC CAGACACAAA TCAAGAGCCT CTATGAGAGC 
ATCGAGGAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Acyl CoA synthetase 5 (ACSL5) ablation in mice increases energy expenditure and insulin sensitivity and delays fat absorption.
Molecular metabolism5(3)210-20(2016 Mar)
Bowman TA,O'Keeffe KR,D'Aquila T,Yan QW,Griffin JD,Killion EA,Salter DM,Mashek DG,Buhman KK,Greenberg AS

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

Targeting of Acyl-CoA synthetase 5 decreases jejunal fatty acid activation with no effect on dietary long-chain fatty acid absorption.
Lipids in health and disease1288(2013 Jun)
Meller N,Morgan ME,Wong WP,Altemus JB,Sehayek E

The Sj?gren-Larsson syndrome gene encodes a hexadecenal dehydrogenase of the sphingosine 1-phosphate degradation pathway.
Molecular cell46(4)461-71(2012 May)
Nakahara K,Ohkuni A,Kitamura T,Abe K,Naganuma T,Ohno Y,Zoeller RA,Kihara A

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Acsl5 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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