Unc13a gene cDNA ORF clone, Mus musculus(house mouse)

mRNA	Protein	Name
XM_017312879.1	XP_017168368.1	protein unc-13 homolog A isoform X7
XM_017312883.2	XP_017168372.1	protein unc-13 homolog A isoform X12
XM_017312877.1	XP_017168366.1	protein unc-13 homolog A isoform X5
XM_017312883.1	XP_017168372.1	protein unc-13 homolog A isoform X11
XM_017312879.2	XP_017168368.1	protein unc-13 homolog A isoform X8
XM_017312880.2	XP_017168369.1	protein unc-13 homolog A isoform X9
NM_001029873.2	NP_001025044.2	protein unc-13 homolog A
XM_017312875.1	XP_017168364.1	protein unc-13 homolog A isoform X2
XM_006509692.3	XP_006509755.1	protein unc-13 homolog A isoform X3
XM_017312878.1	XP_017168367.1	protein unc-13 homolog A isoform X6
XM_017312882.1	XP_017168371.1	protein unc-13 homolog A isoform X10
XM_017312874.2	XP_017168363.1	protein unc-13 homolog A isoform X1
XM_017312875.2	XP_017168364.1	protein unc-13 homolog A isoform X2
XM_006509693.4	XP_006509756.1	protein unc-13 homolog A isoform X4
XM_030243646.1	XP_030099506.1	protein unc-13 homolog A isoform X5
XM_017312878.2	XP_017168367.1	protein unc-13 homolog A isoform X7
XM_017312881.2	XP_017168370.1	protein unc-13 homolog A isoform X10
XM_017312882.2	XP_017168371.1	protein unc-13 homolog A isoform X11
XM_017312874.1	XP_017168363.1	protein unc-13 homolog A isoform X1
XM_006509693.3	XP_006509756.1	protein unc-13 homolog A isoform X4
XM_017312880.1	XP_017168369.1	protein unc-13 homolog A isoform X8
XM_006509692.4	XP_006509755.1	protein unc-13 homolog A isoform X3
XM_017312877.2	XP_017168366.1	protein unc-13 homolog A isoform X6
XM_017312881.1	XP_017168370.1	protein unc-13 homolog A isoform X9

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu41018	XM_006509692.4 Latest version!	Mus musculus unc-13 homolog A (Unc13a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu41019	XM_006509693.4 Latest version!	Mus musculus unc-13 homolog A (Unc13a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu82525	XM_017312874.2 Latest version!	Mus musculus unc-13 homolog A (Unc13a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu82526	XM_017312875.2 Latest version!	Mus musculus unc-13 homolog A (Unc13a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu82527	XM_017312877.2 Latest version!	Mus musculus unc-13 homolog A (Unc13a), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu82528	XM_017312878.2 Latest version!	Mus musculus unc-13 homolog A (Unc13a), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu82529	XM_017312879.2 Latest version!	Mus musculus unc-13 homolog A (Unc13a), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu82530	XM_017312880.2 Latest version!	Mus musculus unc-13 homolog A (Unc13a), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu82531	XM_017312881.2 Latest version!	Mus musculus unc-13 homolog A (Unc13a), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu82532	XM_017312882.2 Latest version!	Mus musculus unc-13 homolog A (Unc13a), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu82533	XM_017312883.2 Latest version!	Mus musculus unc-13 homolog A (Unc13a), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu89888	XM_030243646.1 Latest version!	Mus musculus unc-13 homolog A (Unc13a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu12297	NM_001029873.2 Latest version!	Mus musculus unc-13 homolog A (Unc13a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
View Old Accession Versions >>
OMu82533	XM_017312883.1	Mus musculus unc-13 homolog A (C. elegans) (Unc13a), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OMu82529	XM_017312879.1	Mus musculus unc-13 homolog A (C. elegans) (Unc13a), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OMu82526	XM_017312875.1	Mus musculus unc-13 homolog A (C. elegans) (Unc13a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OMu82528	XM_017312878.1	Mus musculus unc-13 homolog A (C. elegans) (Unc13a), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OMu82532	XM_017312882.1	Mus musculus unc-13 homolog A (C. elegans) (Unc13a), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OMu82525	XM_017312874.1	Mus musculus unc-13 homolog A (C. elegans) (Unc13a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OMu41019	XM_006509693.3	Mus musculus unc-13 homolog A (C. elegans) (Unc13a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OMu82530	XM_017312880.1	Mus musculus unc-13 homolog A (C. elegans) (Unc13a), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OMu41018	XM_006509692.3	Mus musculus unc-13 homolog A (C. elegans) (Unc13a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OMu82527	XM_017312877.1	Mus musculus unc-13 homolog A (C. elegans) (Unc13a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OMu82531	XM_017312881.1	Mus musculus unc-13 homolog A (C. elegans) (Unc13a), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
Hide Old Accession Versions >>

Unc13a ( XM_006509692.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006509755.1 Mus musculus unc-13 homolog A (Unc13a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu41018
Clone ID Related Accession (Same CDS sequence)	XM_006509692.3 , XM_006509692.4
Accession Version	XM_006509692.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5196bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein unc-13 homolog A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006509692.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTGC TGTGCGTGGG AGTCAAAAAA GCCAAGTTTG ACGGTGCCCA AGAGAAGTTC 
AACACATACG TGACGCTGAA GGTGCAGAAC GTGAAGAGCA CTACCATAGC TGTACGTGGC 
AGCCAGCCCA GCTGGGAGCA GGACTTCATG TTTGAGATCA ACCGCCTGGA TCTAGGCCTG 
ACCGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGTAC TGTGTGGATC 
CCGCTGCGGA CCATCCGCCA GTCCAATGAG GAGGGCCCAG GAGAGTGGCT CACACTGGAC 
TCTCAGGCCA TCATGGCGGA CAGTGAGATC TGTGGGACCA AGGACCCCAC CTTCCATCGC 
ATCCTCCTGG ACGCTCATTT CGAGCTGCCG TTGGACATCC CTGAGGAGGA GGCACGCTAC 
TGGGCCAAGA AGCTGGAGCA GCTGAATGCC ATGCGTGACC AGGATGAGTA CTCCTTCCAG 
GACCAGCAGG ACAAGCCGCT GCCAGTGCCC AGCAGCCAGT GCTGCAACTG GAATTACTTT 
GGCTGGGGAG AACAGAATGA CGACCCCGAC AGTGCGGTGG ATGACCGGGA CAGTGACTAT 
AGGAGTGAGA CCAGCAACAG CATCCCACCG CCCTACTATA CGACTTCGCA GCCCAACGCC 
TCTGTGCACC AGTACTCCGT GCGACCGCCC CCTCTGGGGT CCCGGGAGTC CTACAGTGAC 
TCCATGCACA GCTATGAGGA GTTCTCTGAG CCGCGGGCGC TCAGCCCCAC AGGCAGCAGC 
CGCTATGCTT CTTCCGGGGA GCTGAGCCAG GGAAGCTCAC AGCTGAGCGA GGACTTCGAC 
CCTGATGAGC ACAGCCTGCA GGGCTCGGAG CTGGATGACG AGAGGGACCG CGATTCTTAT 
CACTCCTGTC ACAGCTCCGT GAGTTACCAC AAGGACTCAC CCCGCTGGGA CCAGGACGAT 
GAGGACCTGG AGGACCTGGA GGACCTGGAA GATGAGGAGC TGCCCGAGGA GGAGGAGGAG 
TTGGAGGAGG AGGGGGAGGA GGAATTGGAG GAAGAGGATT TGGAGGAGGA GGAGGAGGTG 
CCTGATGACC TGGCCAGCTA CACCCAGCAG GAGGACACCA CTGTGGCTGA GCCCAAAGAG 
TTCAAGCGGA TCAGCTTCCC AACGGCTGCG CCTCAGAAGG ATGACAAGGT TTCAGCTGTG 
CCTACAGAGG CCCCCGAGGT GGCCAAAGGC ATCCCCAAAG CAGCCACACC CGAGGAGAAG 
GCAGCTGCAG AGCGTGCGCA GGAAGCCGAG CCCCCCAAGT CTGAGGAGAG TTTCAGATCT 
CGAGAGGAGG AAGAGGGCCA GGAAGGGCAG GATGCCATGT CCAGAGCCAA AGCCAACTGG 
TTGCGAGCCT TCAACAAGGT GCGCATGCAG CTGCAGGAGG CCCGAGGAGA AGGGGATATG 
TCTAAGTCTC TATGGTTCAA AGGCGGTCCT GGTGGTGGCC TTATCATCAT TGACAGCATG 
CCAGACATCA GGAAACGGAA GCCCATTCCC CTCGTGAGCG ACCTGGCTAT GTCATTGGTG 
CAGTCTCGGA AGGCAGGCAT CACCTCGGCC TTGGCCTCCA GCACGTTGAA CAATGAAGAG 
CTGAAAAATC ACGTTTACAA GAAGACCCTG CAAGCCTTAA TCTACCCCAT CTCCTGCACC 
ACGCCGCACA ATTTCGAGGT GTGGACGGCC ACCACGCCCA CCTACTGCTA TGAGTGCGAG 
GGGCTGCTGT GGGGCATCGC GCGGCAGGGC ATGCGATGCA CCGAGTGCGG CGTTAAGTGC 
CACGAGAAGT GCCAGGACCT GCTCAATGCG GATTGCCTGC AGCGGGCGGC TGAGAAGAGT 
TCCAAGCATG GCGCTGAAGA CCGCACGCAG AACATCATCA TGGTGCTGAA GGACCGCATG 
AAGATCCGCG AGCGCAACAA GCCTGAGATC TTCGAGCTTA TCCAAGAGAT CTTCGCGGTC 
ACCAAGAGCG CACACACACA GCAGATGAAA GCTGTCAAGC AGAGTGTGCT GGACGGCACA 
TCCAAGTGGT CTGCCAAAAT TAGCATCACA GTGGTCTGTG CCCAGGGCTT GCAGGCCAAG 
GACAAGACAG GATCCAGTGA CCCTTATGTC ACCGTCCAGG TTGGGAAGAC CAAGAAAAGG 
ACAAAAACCA TCTACGGGAA CCTCAACCCA GTGTGGGAAG AGAATTTTCA TTTTGAATGT 
CACAACTCCT CTGACCGGAT CAAGGTTCGT GTTTGGGATG AAGATGACGA CATAAAATCC 
CGTGTGAAAC AAAGGTTTAA GAGGGAGTCT GATGACTTCC TAGGGCAGAC AATCATCGAG 
GTGCGGACGC TTAGCGGCGA GATGGATGTG TGGTACAATC TGGACAAGAG AACGGACAAG 
TCGGCGGTGT CGGGGGCCAT CCGGCTTCAC ATCAGTGTGG AGATCAAAGG GGAGGAGAAG 
GTGGCGCCCT ACCATGTCCA GTACACCTGT CTGCATGAGA ACCTGTTCCA CTTTGTGACG 
GACGTGCAGA ACAATGGTGT GGTGAAGATT CCCGATGCCA AGGGTGACGA CGCCTGGAAG 
GTTTACTACG ATGAGACGGC CCAGGAGATC GTGGATGAGT TTGCCATGCG CTATGGTGTT 
GAGTCCATCT ACCAAGCCAT GACCCACTTT GCCTGCCTCT CCTCCAAGTA TATGTGCCCT 
GGGGTACCCG CTGTCATGAG CACCCTGCTT GCCAACATCA ACGCCTACTA CGCACACACC 
ACCGCCTCCA CCAACGTGTC TGCCTCTGAC CGCTTCGCTG CCTCTAATTT CGGGAAAGAG 
CGCTTTGTGA AACTTCTGGA CCAGCTACAC AATTCTCTGC GGATCGACCT GTCCATGTAC 
CGGAACAACT TCCCAGCCAG CAGCCCTGAG CGGCTGCAGG ATCTCAAGTC CACGGTGGAC 
CTGCTCACCA GCATCACCTT CTTCCGGATG AAGGTTCAGG AACTGCAGAG CCCGCCGCGT 
GCCAGCCAGG TAGTGAAAGA CTGTGTGAAG GCCTGCCTCA ACTCCACCTA TGAGTACATC 
TTCAACAACT GCCATGAGCT CTATGGCCGG GAGTACCAAA CGGATCCGGC CAAGAAGGGG 
GAGGTTCCCC CAGAGGAGCA AGGCCCTAGC ATCAAGAACC TGGATTTCTG GTCCAAGCTG 
ATCACCCTCA TCGTGTCTAT CATCGAGGAG GATAAGAATT CTTACACACC CTGCCTCAAT 
CAGTTTCCCC AGGAGCTCAA TGTGGGGAAG ATCAGTGCTG AGGTGATGTG GAGCCTATTT 
GCCCAAGACA TGAAGTACGC CATGGAGGAA CATGACAAAC ACCGGCTGTG TAAGAGCGCA 
GATTACATGA ACCTGCACTT CAAGGTGAAG TGGCTGTACA ATGAGTACGT GGCCGAGCTG 
CCCACCTTCA AGGACCGTGT GCCTGAGTAC CCTGCGTGGT TTGAGCCCTT CGTCATCCAG 
TGGTTGGATG AGAATGAGGA GGTGTCCCGG GACTTCCTGC ATGGTGCCCT CGAGCGGGAC 
AAGAAGGACG GGTTCCAGCA GACCTCAGAG CACGCCCTGT TCTCTTGCTC AGTGGTGGAC 
GTCTTCTCCC AGCTTAACCA GAGTTTCGAG ATCATCAAGA AGCTGGAGTG TCCTGACCCC 
CAGATCGTGG GCCATTACAT GCGGCGTTTT GCCAAGACCA TTAGCAATGT GCTTCTCCAG 
TATGCAGACA TCGTTTCCAA GGACTTCGCC TCCTACTGCT CCAAGGAGAA GGAGAAAGTG 
CCCTGCATCC TCATGAACAA CACACAGCAA CTACGAGTGC AGCTGGAGAA GATGTTTGAG 
GCGATGGGCG GGAAGGAGCT GGATGCCGAG GCCAGTGGCA CCCTGAAGGA GCTGCAGGTG 
AAACTCAACA ATGTCCTGGA TGAACTCAGC CATGTGTTTG CCACCAGCTT CCAGCCACAC 
ATTGAGGAAT GCGTCAGACA GATGGGTGAC ATCCTGAGCC AGGTGAAGGG CACAGGCAAT 
GTACCTGCCA GTGCCTGCAG CAGCGTGGCC CAGGATGCAG ACAATGTGCT GCAGCCCATC 
ATGGATCTTC TGGACAGCAA CCTCACCCTG TTTGCCAAAA TCTGTGAGAA GACAGTTCTG 
AAGCGGGTGC TGAAGGAGCT CTGGAAGCTG GTGATGAACA CAATGGAGAA GACCATCGTC 
CTGCCACCGC TCACTGACCA GACGATGATT GGCACCCTCT TGAGAAAACA TGGCAAGGGC 
CTAGAAAAGG GCAGGGTGAA ACTGCCAAGC CACTCAGACG GGACACAAAT GATCTTCAAT 
GCTGCCAAGG AGCTGGGCCA GCTGTCCAAA CTTAAGGATC ACATGGTGCG GGAAGAAGCC 
AAGAGCTTGA CCCCGAAGCA GTGTGCTGTT GTTGAACTGG CCCTGGACAC CATCAAGCAA 
TACTTCCACG CGGGGGGCGT TGGCCTCAAG AAGACCTTCC TGGAGAAGAG CCCGGACCTC 
CAGTCCCTGC GCTACGCCCT GTCGCTCTAC ACGCAAGCCA CCGACCTGCT CATCAAAACC 
TTCGTGCAGA CGCAGTCAGC GCAGGTCCAT GGTGGAAAAG GGACTAGGTT TACCCTTAGT 
GAAGACGTTT GTCCTGAGAT GGGCTCTGGT GTGGAGGACC CTGTAGGTGA AGTGTCCGTC 
CACGTGGAGC TGTTCACACA TCCAGGAACT GGGGAACAGA AGGTCACAGT GAAGGTGGTG 
GCCGCCAATG ACCTCAAGTG GCAGACTTCT GGCATCTTCC GCCCGTTCAT CGAGGTCAAC 
ATCGTTGGAC CTCAGCTTAG CGACAAGAAA CGCAAGTTCG CCACCAAATC CAAAAACAAC 
AGCTGGGCGC CCAAGTATAA CGAGAGCTTC CAGTTCTCCC TGAGCGCCGA CGCGGGCCCC 
GAGTGCTACG AGCTGCAGGT GTGCGTGAAG GACTACTGCT TTGCGCGCGA AGACCGCACG 
GTGGGGCTGG CGGTGCTGCA ACTGCGAGAG CTGGCCCAGC GCGGGAGCGC CGCGTGCTGG 
CTTCCACTCG GCCGCCGCAT CCACATGGAC GACACGGGGC TCACAGTGCT GCGCATCCTG 
TCGCAGCGCA GCAACGATGA GGTGGCCAAG GAATTCGTCA AGCTCAAGTC GGACACGCGC 
TCAGCCGAGG AGGGCGGTGC CGCGCCTGCG CCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006509755.1
CDS	127..5322
Translation	MSLLCVGVKKAKFDGAQEKFNTYVTLKVQNVKSTTIAVRGSQPSWEQDFMFEINRLDLGLTVEVWNKGLIWDTMVGTVWIPLRTIRQSNEEGPGEWLTLDSQAIMADSEICGTKDPTFHRILLDAHFELPLDIPEEEARYWAKKLEQLNAMRDQDEYSFQDQQDKPLPVPSSQCCNWNYFGWGEQNDDPDSAVDDRDSDYRSETSNSIPPPYYTTSQPNASVHQYSVRPPPLGSRESYSDSMHSYEEFSEPRALSPTGSSRYASSGELSQGSSQLSEDFDPDEHSLQGSELDDERDRDSYHSCHSSVSYHKDSPRWDQDDEDLEDLEDLEDEELPEEEEELEEEGEEELEEEDLEEEEEVPDDLASYTQQEDTTVAEPKEFKRISFPTAAPQKDDKVSAVPTEAPEVAKGIPKAATPEEKAAAERAQEAEPPKSEESFRSREEEEGQEGQDAMSRAKANWLRAFNKVRMQLQEARGEGDMSKSLWFKGGPGGGLIIIDSMPDIRKRKPIPLVSDLAMSLVQSRKAGITSALASSTLNNEELKNHVYKKTLQALIYPISCTTPHNFEVWTATTPTYCYECEGLLWGIARQGMRCTECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMVLKDRMKIRERNKPEIFELIQEIFAVTKSAHTQQMKAVKQSVLDGTSKWSAKISITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIYGNLNPVWEENFHFECHNSSDRIKVRVWDEDDDIKSRVKQRFKRESDDFLGQTIIEVRTLSGEMDVWYNLDKRTDKSAVSGAIRLHISVEIKGEEKVAPYHVQYTCLHENLFHFVTDVQNNGVVKIPDAKGDDAWKVYYDETAQEIVDEFAMRYGVESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSMYRNNFPASSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHELYGREYQTDPAKKGEVPPEEQGPSIKNLDFWSKLITLIVSIIEEDKNSYTPCLNQFPQELNVGKISAEVMWSLFAQDMKYAMEEHDKHRLCKSADYMNLHFKVKWLYNEYVAELPTFKDRVPEYPAWFEPFVIQWLDENEEVSRDFLHGALERDKKDGFQQTSEHALFSCSVVDVFSQLNQSFEIIKKLECPDPQIVGHYMRRFAKTISNVLLQYADIVSKDFASYCSKEKEKVPCILMNNTQQLRVQLEKMFEAMGGKELDAEASGTLKELQVKLNNVLDELSHVFATSFQPHIEECVRQMGDILSQVKGTGNVPASACSSVAQDADNVLQPIMDLLDSNLTLFAKICEKTVLKRVLKELWKLVMNTMEKTIVLPPLTDQTMIGTLLRKHGKGLEKGRVKLPSHSDGTQMIFNAAKELGQLSKLKDHMVREEAKSLTPKQCAVVELALDTIKQYFHAGGVGLKKTFLEKSPDLQSLRYALSLYTQATDLLIKTFVQTQSAQVHGGKGTRFTLSEDVCPEMGSGVEDPVGEVSVHVELFTHPGTGEQKVTVKVVAANDLKWQTSGIFRPFIEVNIVGPQLSDKKRKFATKSKNNSWAPKYNESFQFSLSADAGPECYELQVCVKDYCFAREDRTVGLAVLQLRELAQRGSAACWLPLGRRIHMDDTGLTVLRILSQRSNDEVAKEFVKLKSDTRSAEEGGAAPAP

Target ORF information:

RefSeq Version	XM_006509692.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus unc-13 homolog A (Unc13a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006509692.4

ORF Insert Sequence:

ATGTCTCTGC TGTGCGTGGG AGTCAAAAAA GCCAAGTTTG ACGGTGCCCA AGAGAAGTTC 
AACACATACG TGACGCTGAA GGTGCAGAAC GTGAAGAGCA CTACCATAGC TGTACGTGGC 
AGCCAGCCCA GCTGGGAGCA GGACTTCATG TTTGAGATCA ACCGCCTGGA TCTAGGCCTG 
ACCGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGTAC TGTGTGGATC 
CCGCTGCGGA CCATCCGCCA GTCCAATGAG GAGGGCCCAG GAGAGTGGCT CACACTGGAC 
TCTCAGGCCA TCATGGCGGA CAGTGAGATC TGTGGGACCA AGGACCCCAC CTTCCATCGC 
ATCCTCCTGG ACGCTCATTT CGAGCTGCCG TTGGACATCC CTGAGGAGGA GGCACGCTAC 
TGGGCCAAGA AGCTGGAGCA GCTGAATGCC ATGCGTGACC AGGATGAGTA CTCCTTCCAG 
GACCAGCAGG ACAAGCCGCT GCCAGTGCCC AGCAGCCAGT GCTGCAACTG GAATTACTTT 
GGCTGGGGAG AACAGAATGA CGACCCCGAC AGTGCGGTGG ATGACCGGGA CAGTGACTAT 
AGGAGTGAGA CCAGCAACAG CATCCCACCG CCCTACTATA CGACTTCGCA GCCCAACGCC 
TCTGTGCACC AGTACTCCGT GCGACCGCCC CCTCTGGGGT CCCGGGAGTC CTACAGTGAC 
TCCATGCACA GCTATGAGGA GTTCTCTGAG CCGCGGGCGC TCAGCCCCAC AGGCAGCAGC 
CGCTATGCTT CTTCCGGGGA GCTGAGCCAG GGAAGCTCAC AGCTGAGCGA GGACTTCGAC 
CCTGATGAGC ACAGCCTGCA GGGCTCGGAG CTGGATGACG AGAGGGACCG CGATTCTTAT 
CACTCCTGTC ACAGCTCCGT GAGTTACCAC AAGGACTCAC CCCGCTGGGA CCAGGACGAT 
GAGGACCTGG AGGACCTGGA GGACCTGGAA GATGAGGAGC TGCCCGAGGA GGAGGAGGAG 
TTGGAGGAGG AGGGGGAGGA GGAATTGGAG GAAGAGGATT TGGAGGAGGA GGAGGAGGTG 
CCTGATGACC TGGCCAGCTA CACCCAGCAG GAGGACACCA CTGTGGCTGA GCCCAAAGAG 
TTCAAGCGGA TCAGCTTCCC AACGGCTGCG CCTCAGAAGG ATGACAAGGT TTCAGCTGTG 
CCTACAGAGG CCCCCGAGGT GGCCAAAGGC ATCCCCAAAG CAGCCACACC CGAGGAGAAG 
GCAGCTGCAG AGCGTGCGCA GGAAGCCGAG CCCCCCAAGT CTGAGGAGAG TTTCAGATCT 
CGAGAGGAGG AAGAGGGCCA GGAAGGGCAG GATGCCATGT CCAGAGCCAA AGCCAACTGG 
TTGCGAGCCT TCAACAAGGT GCGCATGCAG CTGCAGGAGG CCCGAGGAGA AGGGGATATG 
TCTAAGTCTC TATGGTTCAA AGGCGGTCCT GGTGGTGGCC TTATCATCAT TGACAGCATG 
CCAGACATCA GGAAACGGAA GCCCATTCCC CTCGTGAGCG ACCTGGCTAT GTCATTGGTG 
CAGTCTCGGA AGGCAGGCAT CACCTCGGCC TTGGCCTCCA GCACGTTGAA CAATGAAGAG 
CTGAAAAATC ACGTTTACAA GAAGACCCTG CAAGCCTTAA TCTACCCCAT CTCCTGCACC 
ACGCCGCACA ATTTCGAGGT GTGGACGGCC ACCACGCCCA CCTACTGCTA TGAGTGCGAG 
GGGCTGCTGT GGGGCATCGC GCGGCAGGGC ATGCGATGCA CCGAGTGCGG CGTTAAGTGC 
CACGAGAAGT GCCAGGACCT GCTCAATGCG GATTGCCTGC AGCGGGCGGC TGAGAAGAGT 
TCCAAGCATG GCGCTGAAGA CCGCACGCAG AACATCATCA TGGTGCTGAA GGACCGCATG 
AAGATCCGCG AGCGCAACAA GCCTGAGATC TTCGAGCTTA TCCAAGAGAT CTTCGCGGTC 
ACCAAGAGCG CACACACACA GCAGATGAAA GCTGTCAAGC AGAGTGTGCT GGACGGCACA 
TCCAAGTGGT CTGCCAAAAT TAGCATCACA GTGGTCTGTG CCCAGGGCTT GCAGGCCAAG 
GACAAGACAG GATCCAGTGA CCCTTATGTC ACCGTCCAGG TTGGGAAGAC CAAGAAAAGG 
ACAAAAACCA TCTACGGGAA CCTCAACCCA GTGTGGGAAG AGAATTTTCA TTTTGAATGT 
CACAACTCCT CTGACCGGAT CAAGGTTCGT GTTTGGGATG AAGATGACGA CATAAAATCC 
CGTGTGAAAC AAAGGTTTAA GAGGGAGTCT GATGACTTCC TAGGGCAGAC AATCATCGAG 
GTGCGGACGC TTAGCGGCGA GATGGATGTG TGGTACAATC TGGACAAGAG AACGGACAAG 
TCGGCGGTGT CGGGGGCCAT CCGGCTTCAC ATCAGTGTGG AGATCAAAGG GGAGGAGAAG 
GTGGCGCCCT ACCATGTCCA GTACACCTGT CTGCATGAGA ACCTGTTCCA CTTTGTGACG 
GACGTGCAGA ACAATGGTGT GGTGAAGATT CCCGATGCCA AGGGTGACGA CGCCTGGAAG 
GTTTACTACG ATGAGACGGC CCAGGAGATC GTGGATGAGT TTGCCATGCG CTATGGTGTT 
GAGTCCATCT ACCAAGCCAT GACCCACTTT GCCTGCCTCT CCTCCAAGTA TATGTGCCCT 
GGGGTACCCG CTGTCATGAG CACCCTGCTT GCCAACATCA ACGCCTACTA CGCACACACC 
ACCGCCTCCA CCAACGTGTC TGCCTCTGAC CGCTTCGCTG CCTCTAATTT CGGGAAAGAG 
CGCTTTGTGA AACTTCTGGA CCAGCTACAC AATTCTCTGC GGATCGACCT GTCCATGTAC 
CGGAACAACT TCCCAGCCAG CAGCCCTGAG CGGCTGCAGG ATCTCAAGTC CACGGTGGAC 
CTGCTCACCA GCATCACCTT CTTCCGGATG AAGGTTCAGG AACTGCAGAG CCCGCCGCGT 
GCCAGCCAGG TAGTGAAAGA CTGTGTGAAG GCCTGCCTCA ACTCCACCTA TGAGTACATC 
TTCAACAACT GCCATGAGCT CTATGGCCGG GAGTACCAAA CGGATCCGGC CAAGAAGGGG 
GAGGTTCCCC CAGAGGAGCA AGGCCCTAGC ATCAAGAACC TGGATTTCTG GTCCAAGCTG 
ATCACCCTCA TCGTGTCTAT CATCGAGGAG GATAAGAATT CTTACACACC CTGCCTCAAT 
CAGTTTCCCC AGGAGCTCAA TGTGGGGAAG ATCAGTGCTG AGGTGATGTG GAGCCTATTT 
GCCCAAGACA TGAAGTACGC CATGGAGGAA CATGACAAAC ACCGGCTGTG TAAGAGCGCA 
GATTACATGA ACCTGCACTT CAAGGTGAAG TGGCTGTACA ATGAGTACGT GGCCGAGCTG 
CCCACCTTCA AGGACCGTGT GCCTGAGTAC CCTGCGTGGT TTGAGCCCTT CGTCATCCAG 
TGGTTGGATG AGAATGAGGA GGTGTCCCGG GACTTCCTGC ATGGTGCCCT CGAGCGGGAC 
AAGAAGGACG GGTTCCAGCA GACCTCAGAG CACGCCCTGT TCTCTTGCTC AGTGGTGGAC 
GTCTTCTCCC AGCTTAACCA GAGTTTCGAG ATCATCAAGA AGCTGGAGTG TCCTGACCCC 
CAGATCGTGG GCCATTACAT GCGGCGTTTT GCCAAGACCA TTAGCAATGT GCTTCTCCAG 
TATGCAGACA TCGTTTCCAA GGACTTCGCC TCCTACTGCT CCAAGGAGAA GGAGAAAGTG 
CCCTGCATCC TCATGAACAA CACACAGCAA CTACGAGTGC AGCTGGAGAA GATGTTTGAG 
GCGATGGGCG GGAAGGAGCT GGATGCCGAG GCCAGTGGCA CCCTGAAGGA GCTGCAGGTG 
AAACTCAACA ATGTCCTGGA TGAACTCAGC CATGTGTTTG CCACCAGCTT CCAGCCACAC 
ATTGAGGAAT GCGTCAGACA GATGGGTGAC ATCCTGAGCC AGGTGAAGGG CACAGGCAAT 
GTACCTGCCA GTGCCTGCAG CAGCGTGGCC CAGGATGCAG ACAATGTGCT GCAGCCCATC 
ATGGATCTTC TGGACAGCAA CCTCACCCTG TTTGCCAAAA TCTGTGAGAA GACAGTTCTG 
AAGCGGGTGC TGAAGGAGCT CTGGAAGCTG GTGATGAACA CAATGGAGAA GACCATCGTC 
CTGCCACCGC TCACTGACCA GACGATGATT GGCACCCTCT TGAGAAAACA TGGCAAGGGC 
CTAGAAAAGG GCAGGGTGAA ACTGCCAAGC CACTCAGACG GGACACAAAT GATCTTCAAT 
GCTGCCAAGG AGCTGGGCCA GCTGTCCAAA CTTAAGGATC ACATGGTGCG GGAAGAAGCC 
AAGAGCTTGA CCCCGAAGCA GTGTGCTGTT GTTGAACTGG CCCTGGACAC CATCAAGCAA 
TACTTCCACG CGGGGGGCGT TGGCCTCAAG AAGACCTTCC TGGAGAAGAG CCCGGACCTC 
CAGTCCCTGC GCTACGCCCT GTCGCTCTAC ACGCAAGCCA CCGACCTGCT CATCAAAACC 
TTCGTGCAGA CGCAGTCAGC GCAGGTCCAT GGTGGAAAAG GGACTAGGTT TACCCTTAGT 
GAAGACGTTT GTCCTGAGAT GGGCTCTGGT GTGGAGGACC CTGTAGGTGA AGTGTCCGTC 
CACGTGGAGC TGTTCACACA TCCAGGAACT GGGGAACAGA AGGTCACAGT GAAGGTGGTG 
GCCGCCAATG ACCTCAAGTG GCAGACTTCT GGCATCTTCC GCCCGTTCAT CGAGGTCAAC 
ATCGTTGGAC CTCAGCTTAG CGACAAGAAA CGCAAGTTCG CCACCAAATC CAAAAACAAC 
AGCTGGGCGC CCAAGTATAA CGAGAGCTTC CAGTTCTCCC TGAGCGCCGA CGCGGGCCCC 
GAGTGCTACG AGCTGCAGGT GTGCGTGAAG GACTACTGCT TTGCGCGCGA AGACCGCACG 
GTGGGGCTGG CGGTGCTGCA ACTGCGAGAG CTGGCCCAGC GCGGGAGCGC CGCGTGCTGG 
CTTCCACTCG GCCGCCGCAT CCACATGGAC GACACGGGGC TCACAGTGCT GCGCATCCTG 
TCGCAGCGCA GCAACGATGA GGTGGCCAAG GAATTCGTCA AGCTCAAGTC GGACACGCGC 
TCAGCCGAGG AGGGCGGTGC CGCGCCTGCG CCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Unc13a ( XM_006509693.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006509756.1 Mus musculus unc-13 homolog A (Unc13a), transcript variant X4, mRNA.

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CloneID	OMu41019
Clone ID Related Accession (Same CDS sequence)	XM_006509693.4 , XM_006509693.3
Accession Version	XM_006509693.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5181bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein unc-13 homolog A isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006509693.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTGC TGTGCGTGGG AGAGAAGTTC AACACATACG TGACGCTGAA GGTGCAGAAC 
GTGAAGAGCA CTACCATAGC TGTACGTGGC AGCCAGCCCA GCTGGGAGCA GGACTTCATG 
TTTGAGATCA ACCGCCTGGA TCTAGGCCTG ACCGTGGAGG TGTGGAACAA GGGTCTCATC 
TGGGACACGA TGGTGGGTAC TGTGTGGATC CCGCTGCGGA CCATCCGCCA GTCCAATGAG 
GAGGGCCCAG GAGAGTGGCT CACACTGGAC TCTCAGGCCA TCATGGCGGA CAGTGAGATC 
TGTGGGACCA AGGACCCCAC CTTCCATCGC ATCCTCCTGG ACGCTCATTT CGAGCTGCCG 
TTGGACATCC CTGAGGAGGA GGCACGCTAC TGGGCCAAGA AGCTGGAGCA GCTGAATGCC 
ATGCGTGACC AGGATGAGGT GAGTCACCTC CAGTACTCCT TCCAGGACCA GCAGGACAAG 
CCGCTGCCAG TGCCCAGCAG CCAGTGCTGC AACTGGAATT ACTTTGGCTG GGGAGAACAG 
AATGACGACC CCGACAGTGC GGTGGATGAC CGGGACAGTG ACTATAGGAG TGAGACCAGC 
AACAGCATCC CACCGCCCTA CTATACGACT TCGCAGCCCA ACGCCTCTGT GCACCAGTAC 
TCCGTGCGAC CGCCCCCTCT GGGGTCCCGG GAGTCCTACA GTGACTCCAT GCACAGCTAT 
GAGGAGTTCT CTGAGCCGCG GGCGCTCAGC CCCACAGGCA GCAGCCGCTA TGCTTCTTCC 
GGGGAGCTGA GCCAGGGAAG CTCACAGCTG AGCGAGGACT TCGACCCTGA TGAGCACAGC 
CTGCAGGGCT CGGAGCTGGA TGACGAGAGG GACCGCGATT CTTATCACTC CTGTCACAGC 
TCCGTGAGTT ACCACAAGGA CTCACCCCGC TGGGACCAGG ACGATGAGGA CCTGGAGGAC 
CTGGAGGACC TGGAAGATGA GGAGCTGCCC GAGGAGGAGG AGGAGTTGGA GGAGGAGGGG 
GAGGAGGAAT TGGAGGAAGA GGATTTGGAG GAGGAGGAGG AGGTGCCTGA TGACCTGGCC 
AGCTACACCC AGCAGGAGGA CACCACTGTG GCTGAGCCCA AAGAGTTCAA GCGGATCAGC 
TTCCCAACGG CTGCGCCTCA GAAGGATGAC AAGGTTTCAG CTGTGCCTAC AGAGGCCCCC 
GAGGTGGCCA AAGGCATCCC CAAAGCAGCC ACACCCGAGG AGAAGGCAGC TGCAGAGCGT 
GCGCAGGAAG CCGAGCCCCC CAAGTCTGAG GAGAGTTTCA GATCTCGAGA GGAGGAAGAG 
GGCCAGGAAG GGCAGGATGC CATGTCCAGA GCCAAAGCCA ACTGGTTGCG AGCCTTCAAC 
AAGGTGCGCA TGCAGCTGCA GGAGGCCCGA GGAGAAGGGG ATATGTCTAA GTCTCTATGG 
TTCAAAGGCG GTCCTGGTGG TGGCCTTATC ATCATTGACA GCATGCCAGA CATCAGGAAA 
CGGAAGCCCA TTCCCCTCGT GAGCGACCTG GCTATGTCAT TGGTGCAGTC TCGGAAGGCA 
GGCATCACCT CGGCCTTGGC CTCCAGCACG TTGAACAATG AAGAGCTGAA AAATCACGTT 
TACAAGAAGA CCCTGCAAGC CTTAATCTAC CCCATCTCCT GCACCACGCC GCACAATTTC 
GAGGTGTGGA CGGCCACCAC GCCCACCTAC TGCTATGAGT GCGAGGGGCT GCTGTGGGGC 
ATCGCGCGGC AGGGCATGCG ATGCACCGAG TGCGGCGTTA AGTGCCACGA GAAGTGCCAG 
GACCTGCTCA ATGCGGATTG CCTGCAGCGG GCGGCTGAGA AGAGTTCCAA GCATGGCGCT 
GAAGACCGCA CGCAGAACAT CATCATGGTG CTGAAGGACC GCATGAAGAT CCGCGAGCGC 
AACAAGCCTG AGATCTTCGA GCTTATCCAA GAGATCTTCG CGGTCACCAA GAGCGCACAC 
ACACAGCAGA TGAAAGCTGT CAAGCAGAGT GTGCTGGACG GCACATCCAA GTGGTCTGCC 
AAAATTAGCA TCACAGTGGT CTGTGCCCAG GGCTTGCAGG CCAAGGACAA GACAGGATCC 
AGTGACCCTT ATGTCACCGT CCAGGTTGGG AAGACCAAGA AAAGGACAAA AACCATCTAC 
GGGAACCTCA ACCCAGTGTG GGAAGAGAAT TTTCATTTTG AATGTCACAA CTCCTCTGAC 
CGGATCAAGG TTCGTGTTTG GGATGAAGAT GACGACATAA AATCCCGTGT GAAACAAAGG 
TTTAAGAGGG AGTCTGATGA CTTCCTAGGG CAGACAATCA TCGAGGTGCG GACGCTTAGC 
GGCGAGATGG ATGTGTGGTA CAATCTGGAC AAGAGAACGG ACAAGTCGGC GGTGTCGGGG 
GCCATCCGGC TTCACATCAG TGTGGAGATC AAAGGGGAGG AGAAGGTGGC GCCCTACCAT 
GTCCAGTACA CCTGTCTGCA TGAGAACCTG TTCCACTTTG TGACGGACGT GCAGAACAAT 
GGTGTGGTGA AGATTCCCGA TGCCAAGGGT GACGACGCCT GGAAGGTTTA CTACGATGAG 
ACGGCCCAGG AGATCGTGGA TGAGTTTGCC ATGCGCTATG GTGTTGAGTC CATCTACCAA 
GCCATGACCC ACTTTGCCTG CCTCTCCTCC AAGTATATGT GCCCTGGGGT ACCCGCTGTC 
ATGAGCACCC TGCTTGCCAA CATCAACGCC TACTACGCAC ACACCACCGC CTCCACCAAC 
GTGTCTGCCT CTGACCGCTT CGCTGCCTCT AATTTCGGGA AAGAGCGCTT TGTGAAACTT 
CTGGACCAGC TACACAATTC TCTGCGGATC GACCTGTCCA TGTACCGGAA CAACTTCCCA 
GCCAGCAGCC CTGAGCGGCT GCAGGATCTC AAGTCCACGG TGGACCTGCT CACCAGCATC 
ACCTTCTTCC GGATGAAGGT TCAGGAACTG CAGAGCCCGC CGCGTGCCAG CCAGGTAGTG 
AAAGACTGTG TGAAGGCCTG CCTCAACTCC ACCTATGAGT ACATCTTCAA CAACTGCCAT 
GAGCTCTATG GCCGGGAGTA CCAAACGGAT CCGGCCAAGA AGGGGGAGGT TCCCCCAGAG 
GAGCAAGGCC CTAGCATCAA GAACCTGGAT TTCTGGTCCA AGCTGATCAC CCTCATCGTG 
TCTATCATCG AGGAGGATAA GAATTCTTAC ACACCCTGCC TCAATCAGTT TCCCCAGGAG 
CTCAATGTGG GGAAGATCAG TGCTGAGGTG ATGTGGAGCC TATTTGCCCA AGACATGAAG 
TACGCCATGG AGGAACATGA CAAACACCGG CTGTGTAAGA GCGCAGATTA CATGAACCTG 
CACTTCAAGG TGAAGTGGCT GTACAATGAG TACGTGGCCG AGCTGCCCAC CTTCAAGGAC 
CGTGTGCCTG AGTACCCTGC GTGGTTTGAG CCCTTCGTCA TCCAGTGGTT GGATGAGAAT 
GAGGAGGTGT CCCGGGACTT CCTGCATGGT GCCCTCGAGC GGGACAAGAA GGACGGGTTC 
CAGCAGACCT CAGAGCACGC CCTGTTCTCT TGCTCAGTGG TGGACGTCTT CTCCCAGCTT 
AACCAGAGTT TCGAGATCAT CAAGAAGCTG GAGTGTCCTG ACCCCCAGAT CGTGGGCCAT 
TACATGCGGC GTTTTGCCAA GACCATTAGC AATGTGCTTC TCCAGTATGC AGACATCGTT 
TCCAAGGACT TCGCCTCCTA CTGCTCCAAG GAGAAGGAGA AAGTGCCCTG CATCCTCATG 
AACAACACAC AGCAACTACG AGTGCAGCTG GAGAAGATGT TTGAGGCGAT GGGCGGGAAG 
GAGCTGGATG CCGAGGCCAG TGGCACCCTG AAGGAGCTGC AGGTGAAACT CAACAATGTC 
CTGGATGAAC TCAGCCATGT GTTTGCCACC AGCTTCCAGC CACACATTGA GGAATGCGTC 
AGACAGATGG GTGACATCCT GAGCCAGGTG AAGGGCACAG GCAATGTACC TGCCAGTGCC 
TGCAGCAGCG TGGCCCAGGA TGCAGACAAT GTGCTGCAGC CCATCATGGA TCTTCTGGAC 
AGCAACCTCA CCCTGTTTGC CAAAATCTGT GAGAAGACAG TTCTGAAGCG GGTGCTGAAG 
GAGCTCTGGA AGCTGGTGAT GAACACAATG GAGAAGACCA TCGTCCTGCC ACCGCTCACT 
GACCAGACGA TGATTGGCAC CCTCTTGAGA AAACATGGCA AGGGCCTAGA AAAGGGCAGG 
GTGAAACTGC CAAGCCACTC AGACGGGACA CAAATGATCT TCAATGCTGC CAAGGAGCTG 
GGCCAGCTGT CCAAACTTAA GGATCACATG GTGCGGGAAG AAGCCAAGAG CTTGACCCCG 
AAGCAGTGTG CTGTTGTTGA ACTGGCCCTG GACACCATCA AGCAATACTT CCACGCGGGG 
GGCGTTGGCC TCAAGAAGAC CTTCCTGGAG AAGAGCCCGG ACCTCCAGTC CCTGCGCTAC 
GCCCTGTCGC TCTACACGCA AGCCACCGAC CTGCTCATCA AAACCTTCGT GCAGACGCAG 
TCAGCGCAGG TCCATGGTGG AAAAGGGACT AGGTTTACCC TTAGTGAAGA CGTTTGTCCT 
GAGATGGGCT CTGGTGTGGA GGACCCTGTA GGTGAAGTGT CCGTCCACGT GGAGCTGTTC 
ACACATCCAG GAACTGGGGA ACAGAAGGTC ACAGTGAAGG TGGTGGCCGC CAATGACCTC 
AAGTGGCAGA CTTCTGGCAT CTTCCGCCCG TTCATCGAGG TCAACATCGT TGGACCTCAG 
CTTAGCGACA AGAAACGCAA GTTCGCCACC AAATCCAAAA ACAACAGCTG GGCGCCCAAG 
TATAACGAGA GCTTCCAGTT CTCCCTGAGC GCCGACGCGG GCCCCGAGTG CTACGAGCTG 
CAGGTGTGCG TGAAGGACTA CTGCTTTGCG CGCGAAGACC GCACGGTGGG GCTGGCGGTG 
CTGCAACTGC GAGAGCTGGC CCAGCGCGGG AGCGCCGCGT GCTGGCTTCC ACTCGGCCGC 
CGCATCCACA TGGACGACAC GGGGCTCACA GTGCTGCGCA TCCTGTCGCA GCGCAGCAAC 
GATGAGGTGG CCAAGGAATT CGTCAAGCTC AAGTCGGACA CGCGCTCAGC CGAGGAGGGC 
GGTGCCGCGC CTGCGCCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006509756.1
CDS	127..5307
Translation	MSLLCVGEKFNTYVTLKVQNVKSTTIAVRGSQPSWEQDFMFEINRLDLGLTVEVWNKGLIWDTMVGTVWIPLRTIRQSNEEGPGEWLTLDSQAIMADSEICGTKDPTFHRILLDAHFELPLDIPEEEARYWAKKLEQLNAMRDQDEVSHLQYSFQDQQDKPLPVPSSQCCNWNYFGWGEQNDDPDSAVDDRDSDYRSETSNSIPPPYYTTSQPNASVHQYSVRPPPLGSRESYSDSMHSYEEFSEPRALSPTGSSRYASSGELSQGSSQLSEDFDPDEHSLQGSELDDERDRDSYHSCHSSVSYHKDSPRWDQDDEDLEDLEDLEDEELPEEEEELEEEGEEELEEEDLEEEEEVPDDLASYTQQEDTTVAEPKEFKRISFPTAAPQKDDKVSAVPTEAPEVAKGIPKAATPEEKAAAERAQEAEPPKSEESFRSREEEEGQEGQDAMSRAKANWLRAFNKVRMQLQEARGEGDMSKSLWFKGGPGGGLIIIDSMPDIRKRKPIPLVSDLAMSLVQSRKAGITSALASSTLNNEELKNHVYKKTLQALIYPISCTTPHNFEVWTATTPTYCYECEGLLWGIARQGMRCTECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMVLKDRMKIRERNKPEIFELIQEIFAVTKSAHTQQMKAVKQSVLDGTSKWSAKISITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIYGNLNPVWEENFHFECHNSSDRIKVRVWDEDDDIKSRVKQRFKRESDDFLGQTIIEVRTLSGEMDVWYNLDKRTDKSAVSGAIRLHISVEIKGEEKVAPYHVQYTCLHENLFHFVTDVQNNGVVKIPDAKGDDAWKVYYDETAQEIVDEFAMRYGVESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSMYRNNFPASSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHELYGREYQTDPAKKGEVPPEEQGPSIKNLDFWSKLITLIVSIIEEDKNSYTPCLNQFPQELNVGKISAEVMWSLFAQDMKYAMEEHDKHRLCKSADYMNLHFKVKWLYNEYVAELPTFKDRVPEYPAWFEPFVIQWLDENEEVSRDFLHGALERDKKDGFQQTSEHALFSCSVVDVFSQLNQSFEIIKKLECPDPQIVGHYMRRFAKTISNVLLQYADIVSKDFASYCSKEKEKVPCILMNNTQQLRVQLEKMFEAMGGKELDAEASGTLKELQVKLNNVLDELSHVFATSFQPHIEECVRQMGDILSQVKGTGNVPASACSSVAQDADNVLQPIMDLLDSNLTLFAKICEKTVLKRVLKELWKLVMNTMEKTIVLPPLTDQTMIGTLLRKHGKGLEKGRVKLPSHSDGTQMIFNAAKELGQLSKLKDHMVREEAKSLTPKQCAVVELALDTIKQYFHAGGVGLKKTFLEKSPDLQSLRYALSLYTQATDLLIKTFVQTQSAQVHGGKGTRFTLSEDVCPEMGSGVEDPVGEVSVHVELFTHPGTGEQKVTVKVVAANDLKWQTSGIFRPFIEVNIVGPQLSDKKRKFATKSKNNSWAPKYNESFQFSLSADAGPECYELQVCVKDYCFAREDRTVGLAVLQLRELAQRGSAACWLPLGRRIHMDDTGLTVLRILSQRSNDEVAKEFVKLKSDTRSAEEGGAAPAP

Target ORF information:

RefSeq Version	XM_006509693.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus unc-13 homolog A (Unc13a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006509693.4

ORF Insert Sequence:

ATGTCTCTGC TGTGCGTGGG AGAGAAGTTC AACACATACG TGACGCTGAA GGTGCAGAAC 
GTGAAGAGCA CTACCATAGC TGTACGTGGC AGCCAGCCCA GCTGGGAGCA GGACTTCATG 
TTTGAGATCA ACCGCCTGGA TCTAGGCCTG ACCGTGGAGG TGTGGAACAA GGGTCTCATC 
TGGGACACGA TGGTGGGTAC TGTGTGGATC CCGCTGCGGA CCATCCGCCA GTCCAATGAG 
GAGGGCCCAG GAGAGTGGCT CACACTGGAC TCTCAGGCCA TCATGGCGGA CAGTGAGATC 
TGTGGGACCA AGGACCCCAC CTTCCATCGC ATCCTCCTGG ACGCTCATTT CGAGCTGCCG 
TTGGACATCC CTGAGGAGGA GGCACGCTAC TGGGCCAAGA AGCTGGAGCA GCTGAATGCC 
ATGCGTGACC AGGATGAGGT GAGTCACCTC CAGTACTCCT TCCAGGACCA GCAGGACAAG 
CCGCTGCCAG TGCCCAGCAG CCAGTGCTGC AACTGGAATT ACTTTGGCTG GGGAGAACAG 
AATGACGACC CCGACAGTGC GGTGGATGAC CGGGACAGTG ACTATAGGAG TGAGACCAGC 
AACAGCATCC CACCGCCCTA CTATACGACT TCGCAGCCCA ACGCCTCTGT GCACCAGTAC 
TCCGTGCGAC CGCCCCCTCT GGGGTCCCGG GAGTCCTACA GTGACTCCAT GCACAGCTAT 
GAGGAGTTCT CTGAGCCGCG GGCGCTCAGC CCCACAGGCA GCAGCCGCTA TGCTTCTTCC 
GGGGAGCTGA GCCAGGGAAG CTCACAGCTG AGCGAGGACT TCGACCCTGA TGAGCACAGC 
CTGCAGGGCT CGGAGCTGGA TGACGAGAGG GACCGCGATT CTTATCACTC CTGTCACAGC 
TCCGTGAGTT ACCACAAGGA CTCACCCCGC TGGGACCAGG ACGATGAGGA CCTGGAGGAC 
CTGGAGGACC TGGAAGATGA GGAGCTGCCC GAGGAGGAGG AGGAGTTGGA GGAGGAGGGG 
GAGGAGGAAT TGGAGGAAGA GGATTTGGAG GAGGAGGAGG AGGTGCCTGA TGACCTGGCC 
AGCTACACCC AGCAGGAGGA CACCACTGTG GCTGAGCCCA AAGAGTTCAA GCGGATCAGC 
TTCCCAACGG CTGCGCCTCA GAAGGATGAC AAGGTTTCAG CTGTGCCTAC AGAGGCCCCC 
GAGGTGGCCA AAGGCATCCC CAAAGCAGCC ACACCCGAGG AGAAGGCAGC TGCAGAGCGT 
GCGCAGGAAG CCGAGCCCCC CAAGTCTGAG GAGAGTTTCA GATCTCGAGA GGAGGAAGAG 
GGCCAGGAAG GGCAGGATGC CATGTCCAGA GCCAAAGCCA ACTGGTTGCG AGCCTTCAAC 
AAGGTGCGCA TGCAGCTGCA GGAGGCCCGA GGAGAAGGGG ATATGTCTAA GTCTCTATGG 
TTCAAAGGCG GTCCTGGTGG TGGCCTTATC ATCATTGACA GCATGCCAGA CATCAGGAAA 
CGGAAGCCCA TTCCCCTCGT GAGCGACCTG GCTATGTCAT TGGTGCAGTC TCGGAAGGCA 
GGCATCACCT CGGCCTTGGC CTCCAGCACG TTGAACAATG AAGAGCTGAA AAATCACGTT 
TACAAGAAGA CCCTGCAAGC CTTAATCTAC CCCATCTCCT GCACCACGCC GCACAATTTC 
GAGGTGTGGA CGGCCACCAC GCCCACCTAC TGCTATGAGT GCGAGGGGCT GCTGTGGGGC 
ATCGCGCGGC AGGGCATGCG ATGCACCGAG TGCGGCGTTA AGTGCCACGA GAAGTGCCAG 
GACCTGCTCA ATGCGGATTG CCTGCAGCGG GCGGCTGAGA AGAGTTCCAA GCATGGCGCT 
GAAGACCGCA CGCAGAACAT CATCATGGTG CTGAAGGACC GCATGAAGAT CCGCGAGCGC 
AACAAGCCTG AGATCTTCGA GCTTATCCAA GAGATCTTCG CGGTCACCAA GAGCGCACAC 
ACACAGCAGA TGAAAGCTGT CAAGCAGAGT GTGCTGGACG GCACATCCAA GTGGTCTGCC 
AAAATTAGCA TCACAGTGGT CTGTGCCCAG GGCTTGCAGG CCAAGGACAA GACAGGATCC 
AGTGACCCTT ATGTCACCGT CCAGGTTGGG AAGACCAAGA AAAGGACAAA AACCATCTAC 
GGGAACCTCA ACCCAGTGTG GGAAGAGAAT TTTCATTTTG AATGTCACAA CTCCTCTGAC 
CGGATCAAGG TTCGTGTTTG GGATGAAGAT GACGACATAA AATCCCGTGT GAAACAAAGG 
TTTAAGAGGG AGTCTGATGA CTTCCTAGGG CAGACAATCA TCGAGGTGCG GACGCTTAGC 
GGCGAGATGG ATGTGTGGTA CAATCTGGAC AAGAGAACGG ACAAGTCGGC GGTGTCGGGG 
GCCATCCGGC TTCACATCAG TGTGGAGATC AAAGGGGAGG AGAAGGTGGC GCCCTACCAT 
GTCCAGTACA CCTGTCTGCA TGAGAACCTG TTCCACTTTG TGACGGACGT GCAGAACAAT 
GGTGTGGTGA AGATTCCCGA TGCCAAGGGT GACGACGCCT GGAAGGTTTA CTACGATGAG 
ACGGCCCAGG AGATCGTGGA TGAGTTTGCC ATGCGCTATG GTGTTGAGTC CATCTACCAA 
GCCATGACCC ACTTTGCCTG CCTCTCCTCC AAGTATATGT GCCCTGGGGT ACCCGCTGTC 
ATGAGCACCC TGCTTGCCAA CATCAACGCC TACTACGCAC ACACCACCGC CTCCACCAAC 
GTGTCTGCCT CTGACCGCTT CGCTGCCTCT AATTTCGGGA AAGAGCGCTT TGTGAAACTT 
CTGGACCAGC TACACAATTC TCTGCGGATC GACCTGTCCA TGTACCGGAA CAACTTCCCA 
GCCAGCAGCC CTGAGCGGCT GCAGGATCTC AAGTCCACGG TGGACCTGCT CACCAGCATC 
ACCTTCTTCC GGATGAAGGT TCAGGAACTG CAGAGCCCGC CGCGTGCCAG CCAGGTAGTG 
AAAGACTGTG TGAAGGCCTG CCTCAACTCC ACCTATGAGT ACATCTTCAA CAACTGCCAT 
GAGCTCTATG GCCGGGAGTA CCAAACGGAT CCGGCCAAGA AGGGGGAGGT TCCCCCAGAG 
GAGCAAGGCC CTAGCATCAA GAACCTGGAT TTCTGGTCCA AGCTGATCAC CCTCATCGTG 
TCTATCATCG AGGAGGATAA GAATTCTTAC ACACCCTGCC TCAATCAGTT TCCCCAGGAG 
CTCAATGTGG GGAAGATCAG TGCTGAGGTG ATGTGGAGCC TATTTGCCCA AGACATGAAG 
TACGCCATGG AGGAACATGA CAAACACCGG CTGTGTAAGA GCGCAGATTA CATGAACCTG 
CACTTCAAGG TGAAGTGGCT GTACAATGAG TACGTGGCCG AGCTGCCCAC CTTCAAGGAC 
CGTGTGCCTG AGTACCCTGC GTGGTTTGAG CCCTTCGTCA TCCAGTGGTT GGATGAGAAT 
GAGGAGGTGT CCCGGGACTT CCTGCATGGT GCCCTCGAGC GGGACAAGAA GGACGGGTTC 
CAGCAGACCT CAGAGCACGC CCTGTTCTCT TGCTCAGTGG TGGACGTCTT CTCCCAGCTT 
AACCAGAGTT TCGAGATCAT CAAGAAGCTG GAGTGTCCTG ACCCCCAGAT CGTGGGCCAT 
TACATGCGGC GTTTTGCCAA GACCATTAGC AATGTGCTTC TCCAGTATGC AGACATCGTT 
TCCAAGGACT TCGCCTCCTA CTGCTCCAAG GAGAAGGAGA AAGTGCCCTG CATCCTCATG 
AACAACACAC AGCAACTACG AGTGCAGCTG GAGAAGATGT TTGAGGCGAT GGGCGGGAAG 
GAGCTGGATG CCGAGGCCAG TGGCACCCTG AAGGAGCTGC AGGTGAAACT CAACAATGTC 
CTGGATGAAC TCAGCCATGT GTTTGCCACC AGCTTCCAGC CACACATTGA GGAATGCGTC 
AGACAGATGG GTGACATCCT GAGCCAGGTG AAGGGCACAG GCAATGTACC TGCCAGTGCC 
TGCAGCAGCG TGGCCCAGGA TGCAGACAAT GTGCTGCAGC CCATCATGGA TCTTCTGGAC 
AGCAACCTCA CCCTGTTTGC CAAAATCTGT GAGAAGACAG TTCTGAAGCG GGTGCTGAAG 
GAGCTCTGGA AGCTGGTGAT GAACACAATG GAGAAGACCA TCGTCCTGCC ACCGCTCACT 
GACCAGACGA TGATTGGCAC CCTCTTGAGA AAACATGGCA AGGGCCTAGA AAAGGGCAGG 
GTGAAACTGC CAAGCCACTC AGACGGGACA CAAATGATCT TCAATGCTGC CAAGGAGCTG 
GGCCAGCTGT CCAAACTTAA GGATCACATG GTGCGGGAAG AAGCCAAGAG CTTGACCCCG 
AAGCAGTGTG CTGTTGTTGA ACTGGCCCTG GACACCATCA AGCAATACTT CCACGCGGGG 
GGCGTTGGCC TCAAGAAGAC CTTCCTGGAG AAGAGCCCGG ACCTCCAGTC CCTGCGCTAC 
GCCCTGTCGC TCTACACGCA AGCCACCGAC CTGCTCATCA AAACCTTCGT GCAGACGCAG 
TCAGCGCAGG TCCATGGTGG AAAAGGGACT AGGTTTACCC TTAGTGAAGA CGTTTGTCCT 
GAGATGGGCT CTGGTGTGGA GGACCCTGTA GGTGAAGTGT CCGTCCACGT GGAGCTGTTC 
ACACATCCAG GAACTGGGGA ACAGAAGGTC ACAGTGAAGG TGGTGGCCGC CAATGACCTC 
AAGTGGCAGA CTTCTGGCAT CTTCCGCCCG TTCATCGAGG TCAACATCGT TGGACCTCAG 
CTTAGCGACA AGAAACGCAA GTTCGCCACC AAATCCAAAA ACAACAGCTG GGCGCCCAAG 
TATAACGAGA GCTTCCAGTT CTCCCTGAGC GCCGACGCGG GCCCCGAGTG CTACGAGCTG 
CAGGTGTGCG TGAAGGACTA CTGCTTTGCG CGCGAAGACC GCACGGTGGG GCTGGCGGTG 
CTGCAACTGC GAGAGCTGGC CCAGCGCGGG AGCGCCGCGT GCTGGCTTCC ACTCGGCCGC 
CGCATCCACA TGGACGACAC GGGGCTCACA GTGCTGCGCA TCCTGTCGCA GCGCAGCAAC 
GATGAGGTGG CCAAGGAATT CGTCAAGCTC AAGTCGGACA CGCGCTCAGC CGAGGAGGGC 
GGTGCCGCGC CTGCGCCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Unc13a ( XM_017312874.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168363.1 Mus musculus unc-13 homolog A (Unc13a), transcript variant X1, mRNA.

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CloneID	OMu82525
Clone ID Related Accession (Same CDS sequence)	XM_017312874.2 , XM_017312874.1
Accession Version	XM_017312874.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5211bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein unc-13 homolog A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017312874.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTGC TGTGCGTGGG AGTCAAAAAA GCCAAGTTTG ACGGTGCCCA AGAGAAGTTC 
AACACATACG TGACGCTGAA GGTGCAGAAC GTGAAGAGCA CTACCATAGC TGTACGTGGC 
AGCCAGCCCA GCTGGGAGCA GGACTTCATG TTTGAGATCA ACCGCCTGGA TCTAGGCCTG 
ACCGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGTAC TGTGTGGATC 
CCGCTGCGGA CCATCCGCCA GTCCAATGAG GAGGGCCCAG GAGAGTGGCT CACACTGGAC 
TCTCAGGCCA TCATGGCGGA CAGTGAGATC TGTGGGACCA AGGACCCCAC CTTCCATCGC 
ATCCTCCTGG ACGCTCATTT CGAGCTGCCG TTGGACATCC CTGAGGAGGA GGCACGCTAC 
TGGGCCAAGA AGCTGGAGCA GCTGAATGCC ATGCGTGACC AGGATGAGGT GAGTCACCTC 
CAGTACTCCT TCCAGGACCA GCAGGACAAG CCGCTGCCAG TGCCCAGCAG CCAGTGCTGC 
AACTGGAATT ACTTTGGCTG GGGAGAACAG AATGACGACC CCGACAGTGC GGTGGATGAC 
CGGGACAGTG ACTATAGGAG TGAGACCAGC AACAGCATCC CACCGCCCTA CTATACGACT 
TCGCAGCCCA ACGCCTCTGT GCACCAGTAC TCCGTGCGAC CGCCCCCTCT GGGGTCCCGG 
GAGTCCTACA GTGACTCCAT GCACAGCTAT GAGGAGTTCT CTGAGCCGCG GGCGCTCAGC 
CCCACAGGCA GCAGCCGCTA TGCTTCTTCC GGGGAGCTGA GCCAGGGAAG CTCACAGCTG 
AGCGAGGACT TCGACCCTGA TGAGCACAGC CTGCAGGGCT CGGAGCTGGA TGACGAGAGG 
GACCGCGATT CTTATCACTC CTGTCACAGC TCCGTGAGTT ACCACAAGGA CTCACCCCGC 
TGGGACCAGG ACGATGAGGA CCTGGAGGAC CTGGAGGACC TGGAAGATGA GGAGCTGCCC 
GAGGAGGAGG AGGAGTTGGA GGAGGAGGGG GAGGAGGAAT TGGAGGAAGA GGATTTGGAG 
GAGGAGGAGG AGGTGCCTGA TGACCTGGCC AGCTACACCC AGCAGGAGGA CACCACTGTG 
GCTGAGCCCA AAGAGTTCAA GCGGATCAGC TTCCCAACGG CTGCGCCTCA GAAGGATGAC 
AAGGTTTCAG CTGTGCCTAC AGAGGCCCCC GAGGTGGCCA AAGGCATCCC CAAAGCAGCC 
ACACCCGAGG AGAAGGCAGC TGCAGAGCGT GCGCAGGAAG CCGAGCCCCC CAAGTCTGAG 
GAGAGTTTCA GATCTCGAGA GGAGGAAGAG GGCCAGGAAG GGCAGGATGC CATGTCCAGA 
GCCAAAGCCA ACTGGTTGCG AGCCTTCAAC AAGGTGCGCA TGCAGCTGCA GGAGGCCCGA 
GGAGAAGGGG ATATGTCTAA GTCTCTATGG TTCAAAGGCG GTCCTGGTGG TGGCCTTATC 
ATCATTGACA GCATGCCAGA CATCAGGAAA CGGAAGCCCA TTCCCCTCGT GAGCGACCTG 
GCTATGTCAT TGGTGCAGTC TCGGAAGGCA GGCATCACCT CGGCCTTGGC CTCCAGCACG 
TTGAACAATG AAGAGCTGAA AAATCACGTT TACAAGAAGA CCCTGCAAGC CTTAATCTAC 
CCCATCTCCT GCACCACGCC GCACAATTTC GAGGTGTGGA CGGCCACCAC GCCCACCTAC 
TGCTATGAGT GCGAGGGGCT GCTGTGGGGC ATCGCGCGGC AGGGCATGCG ATGCACCGAG 
TGCGGCGTTA AGTGCCACGA GAAGTGCCAG GACCTGCTCA ATGCGGATTG CCTGCAGCGG 
GCGGCTGAGA AGAGTTCCAA GCATGGCGCT GAAGACCGCA CGCAGAACAT CATCATGGTG 
CTGAAGGACC GCATGAAGAT CCGCGAGCGC AACAAGCCTG AGATCTTCGA GCTTATCCAA 
GAGATCTTCG CGGTCACCAA GAGCGCACAC ACACAGCAGA TGAAAGCTGT CAAGCAGAGT 
GTGCTGGACG GCACATCCAA GTGGTCTGCC AAAATTAGCA TCACAGTGGT CTGTGCCCAG 
GGCTTGCAGG CCAAGGACAA GACAGGATCC AGTGACCCTT ATGTCACCGT CCAGGTTGGG 
AAGACCAAGA AAAGGACAAA AACCATCTAC GGGAACCTCA ACCCAGTGTG GGAAGAGAAT 
TTTCATTTTG AATGTCACAA CTCCTCTGAC CGGATCAAGG TTCGTGTTTG GGATGAAGAT 
GACGACATAA AATCCCGTGT GAAACAAAGG TTTAAGAGGG AGTCTGATGA CTTCCTAGGG 
CAGACAATCA TCGAGGTGCG GACGCTTAGC GGCGAGATGG ATGTGTGGTA CAATCTGGAC 
AAGAGAACGG ACAAGTCGGC GGTGTCGGGG GCCATCCGGC TTCACATCAG TGTGGAGATC 
AAAGGGGAGG AGAAGGTGGC GCCCTACCAT GTCCAGTACA CCTGTCTGCA TGAGAACCTG 
TTCCACTTTG TGACGGACGT GCAGAACAAT GGTGTGGTGA AGATTCCCGA TGCCAAGGGT 
GACGACGCCT GGAAGGTTTA CTACGATGAG ACGGCCCAGG AGATCGTGGA TGAGTTTGCC 
ATGCGCTATG GTGTTGAGTC CATCTACCAA GCCATGACCC ACTTTGCCTG CCTCTCCTCC 
AAGTATATGT GCCCTGGGGT ACCCGCTGTC ATGAGCACCC TGCTTGCCAA CATCAACGCC 
TACTACGCAC ACACCACCGC CTCCACCAAC GTGTCTGCCT CTGACCGCTT CGCTGCCTCT 
AATTTCGGGA AAGAGCGCTT TGTGAAACTT CTGGACCAGC TACACAATTC TCTGCGGATC 
GACCTGTCCA TGTACCGGAA CAACTTCCCA GCCAGCAGCC CTGAGCGGCT GCAGGATCTC 
AAGTCCACGG TGGACCTGCT CACCAGCATC ACCTTCTTCC GGATGAAGGT TCAGGAACTG 
CAGAGCCCGC CGCGTGCCAG CCAGGTAGTG AAAGACTGTG TGAAGGCCTG CCTCAACTCC 
ACCTATGAGT ACATCTTCAA CAACTGCCAT GAGCTCTATG GCCGGGAGTA CCAAACGGAT 
CCGGCCAAGA AGGGGGAGGT TCCCCCAGAG GAGCAAGGCC CTAGCATCAA GAACCTGGAT 
TTCTGGTCCA AGCTGATCAC CCTCATCGTG TCTATCATCG AGGAGGATAA GAATTCTTAC 
ACACCCTGCC TCAATCAGTT TCCCCAGGAG CTCAATGTGG GGAAGATCAG TGCTGAGGTG 
ATGTGGAGCC TATTTGCCCA AGACATGAAG TACGCCATGG AGGAACATGA CAAACACCGG 
CTGTGTAAGA GCGCAGATTA CATGAACCTG CACTTCAAGG TGAAGTGGCT GTACAATGAG 
TACGTGGCCG AGCTGCCCAC CTTCAAGGAC CGTGTGCCTG AGTACCCTGC GTGGTTTGAG 
CCCTTCGTCA TCCAGTGGTT GGATGAGAAT GAGGAGGTGT CCCGGGACTT CCTGCATGGT 
GCCCTCGAGC GGGACAAGAA GGACGGGTTC CAGCAGACCT CAGAGCACGC CCTGTTCTCT 
TGCTCAGTGG TGGACGTCTT CTCCCAGCTT AACCAGAGTT TCGAGATCAT CAAGAAGCTG 
GAGTGTCCTG ACCCCCAGAT CGTGGGCCAT TACATGCGGC GTTTTGCCAA GACCATTAGC 
AATGTGCTTC TCCAGTATGC AGACATCGTT TCCAAGGACT TCGCCTCCTA CTGCTCCAAG 
GAGAAGGAGA AAGTGCCCTG CATCCTCATG AACAACACAC AGCAACTACG AGTGCAGCTG 
GAGAAGATGT TTGAGGCGAT GGGCGGGAAG GAGCTGGATG CCGAGGCCAG TGGCACCCTG 
AAGGAGCTGC AGGTGAAACT CAACAATGTC CTGGATGAAC TCAGCCATGT GTTTGCCACC 
AGCTTCCAGC CACACATTGA GGAATGCGTC AGACAGATGG GTGACATCCT GAGCCAGGTG 
AAGGGCACAG GCAATGTACC TGCCAGTGCC TGCAGCAGCG TGGCCCAGGA TGCAGACAAT 
GTGCTGCAGC CCATCATGGA TCTTCTGGAC AGCAACCTCA CCCTGTTTGC CAAAATCTGT 
GAGAAGACAG TTCTGAAGCG GGTGCTGAAG GAGCTCTGGA AGCTGGTGAT GAACACAATG 
GAGAAGACCA TCGTCCTGCC ACCGCTCACT GACCAGACGA TGATTGGCAC CCTCTTGAGA 
AAACATGGCA AGGGCCTAGA AAAGGGCAGG GTGAAACTGC CAAGCCACTC AGACGGGACA 
CAAATGATCT TCAATGCTGC CAAGGAGCTG GGCCAGCTGT CCAAACTTAA GGATCACATG 
GTGCGGGAAG AAGCCAAGAG CTTGACCCCG AAGCAGTGTG CTGTTGTTGA ACTGGCCCTG 
GACACCATCA AGCAATACTT CCACGCGGGG GGCGTTGGCC TCAAGAAGAC CTTCCTGGAG 
AAGAGCCCGG ACCTCCAGTC CCTGCGCTAC GCCCTGTCGC TCTACACGCA AGCCACCGAC 
CTGCTCATCA AAACCTTCGT GCAGACGCAG TCAGCGCAGG TCCATGGTGG AAAAGGGACT 
AGGTTTACCC TTAGTGAAGA CGTTTGTCCT GAGATGGGCT CTGGTGTGGA GGACCCTGTA 
GGTGAAGTGT CCGTCCACGT GGAGCTGTTC ACACATCCAG GAACTGGGGA ACAGAAGGTC 
ACAGTGAAGG TGGTGGCCGC CAATGACCTC AAGTGGCAGA CTTCTGGCAT CTTCCGCCCG 
TTCATCGAGG TCAACATCGT TGGACCTCAG CTTAGCGACA AGAAACGCAA GTTCGCCACC 
AAATCCAAAA ACAACAGCTG GGCGCCCAAG TATAACGAGA GCTTCCAGTT CTCCCTGAGC 
GCCGACGCGG GCCCCGAGTG CTACGAGCTG CAGGTGTGCG TGAAGGACTA CTGCTTTGCG 
CGCGAAGACC GCACGGTGGG GCTGGCGGTG CTGCAACTGC GAGAGCTGGC CCAGCGCGGG 
AGCGCCGCGT GCTGGCTTCC ACTCGGCCGC CGCATCCACA TGGACGACAC GGGGCTCACA 
GTGCTGCGCA TCCTGTCGCA GCGCAGCAAC GATGAGGTGG CCAAGGAATT CGTCAAGCTC 
AAGTCGGACA CGCGCTCAGC CGAGGAGGGC GGTGCCGCGC CTGCGCCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168363.1
CDS	127..5337
Translation	MSLLCVGVKKAKFDGAQEKFNTYVTLKVQNVKSTTIAVRGSQPSWEQDFMFEINRLDLGLTVEVWNKGLIWDTMVGTVWIPLRTIRQSNEEGPGEWLTLDSQAIMADSEICGTKDPTFHRILLDAHFELPLDIPEEEARYWAKKLEQLNAMRDQDEVSHLQYSFQDQQDKPLPVPSSQCCNWNYFGWGEQNDDPDSAVDDRDSDYRSETSNSIPPPYYTTSQPNASVHQYSVRPPPLGSRESYSDSMHSYEEFSEPRALSPTGSSRYASSGELSQGSSQLSEDFDPDEHSLQGSELDDERDRDSYHSCHSSVSYHKDSPRWDQDDEDLEDLEDLEDEELPEEEEELEEEGEEELEEEDLEEEEEVPDDLASYTQQEDTTVAEPKEFKRISFPTAAPQKDDKVSAVPTEAPEVAKGIPKAATPEEKAAAERAQEAEPPKSEESFRSREEEEGQEGQDAMSRAKANWLRAFNKVRMQLQEARGEGDMSKSLWFKGGPGGGLIIIDSMPDIRKRKPIPLVSDLAMSLVQSRKAGITSALASSTLNNEELKNHVYKKTLQALIYPISCTTPHNFEVWTATTPTYCYECEGLLWGIARQGMRCTECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMVLKDRMKIRERNKPEIFELIQEIFAVTKSAHTQQMKAVKQSVLDGTSKWSAKISITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIYGNLNPVWEENFHFECHNSSDRIKVRVWDEDDDIKSRVKQRFKRESDDFLGQTIIEVRTLSGEMDVWYNLDKRTDKSAVSGAIRLHISVEIKGEEKVAPYHVQYTCLHENLFHFVTDVQNNGVVKIPDAKGDDAWKVYYDETAQEIVDEFAMRYGVESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSMYRNNFPASSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHELYGREYQTDPAKKGEVPPEEQGPSIKNLDFWSKLITLIVSIIEEDKNSYTPCLNQFPQELNVGKISAEVMWSLFAQDMKYAMEEHDKHRLCKSADYMNLHFKVKWLYNEYVAELPTFKDRVPEYPAWFEPFVIQWLDENEEVSRDFLHGALERDKKDGFQQTSEHALFSCSVVDVFSQLNQSFEIIKKLECPDPQIVGHYMRRFAKTISNVLLQYADIVSKDFASYCSKEKEKVPCILMNNTQQLRVQLEKMFEAMGGKELDAEASGTLKELQVKLNNVLDELSHVFATSFQPHIEECVRQMGDILSQVKGTGNVPASACSSVAQDADNVLQPIMDLLDSNLTLFAKICEKTVLKRVLKELWKLVMNTMEKTIVLPPLTDQTMIGTLLRKHGKGLEKGRVKLPSHSDGTQMIFNAAKELGQLSKLKDHMVREEAKSLTPKQCAVVELALDTIKQYFHAGGVGLKKTFLEKSPDLQSLRYALSLYTQATDLLIKTFVQTQSAQVHGGKGTRFTLSEDVCPEMGSGVEDPVGEVSVHVELFTHPGTGEQKVTVKVVAANDLKWQTSGIFRPFIEVNIVGPQLSDKKRKFATKSKNNSWAPKYNESFQFSLSADAGPECYELQVCVKDYCFAREDRTVGLAVLQLRELAQRGSAACWLPLGRRIHMDDTGLTVLRILSQRSNDEVAKEFVKLKSDTRSAEEGGAAPAP

Target ORF information:

RefSeq Version	XM_017312874.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus unc-13 homolog A (Unc13a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312874.2

ORF Insert Sequence:

ATGTCTCTGC TGTGCGTGGG AGTCAAAAAA GCCAAGTTTG ACGGTGCCCA AGAGAAGTTC 
AACACATACG TGACGCTGAA GGTGCAGAAC GTGAAGAGCA CTACCATAGC TGTACGTGGC 
AGCCAGCCCA GCTGGGAGCA GGACTTCATG TTTGAGATCA ACCGCCTGGA TCTAGGCCTG 
ACCGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGTAC TGTGTGGATC 
CCGCTGCGGA CCATCCGCCA GTCCAATGAG GAGGGCCCAG GAGAGTGGCT CACACTGGAC 
TCTCAGGCCA TCATGGCGGA CAGTGAGATC TGTGGGACCA AGGACCCCAC CTTCCATCGC 
ATCCTCCTGG ACGCTCATTT CGAGCTGCCG TTGGACATCC CTGAGGAGGA GGCACGCTAC 
TGGGCCAAGA AGCTGGAGCA GCTGAATGCC ATGCGTGACC AGGATGAGGT GAGTCACCTC 
CAGTACTCCT TCCAGGACCA GCAGGACAAG CCGCTGCCAG TGCCCAGCAG CCAGTGCTGC 
AACTGGAATT ACTTTGGCTG GGGAGAACAG AATGACGACC CCGACAGTGC GGTGGATGAC 
CGGGACAGTG ACTATAGGAG TGAGACCAGC AACAGCATCC CACCGCCCTA CTATACGACT 
TCGCAGCCCA ACGCCTCTGT GCACCAGTAC TCCGTGCGAC CGCCCCCTCT GGGGTCCCGG 
GAGTCCTACA GTGACTCCAT GCACAGCTAT GAGGAGTTCT CTGAGCCGCG GGCGCTCAGC 
CCCACAGGCA GCAGCCGCTA TGCTTCTTCC GGGGAGCTGA GCCAGGGAAG CTCACAGCTG 
AGCGAGGACT TCGACCCTGA TGAGCACAGC CTGCAGGGCT CGGAGCTGGA TGACGAGAGG 
GACCGCGATT CTTATCACTC CTGTCACAGC TCCGTGAGTT ACCACAAGGA CTCACCCCGC 
TGGGACCAGG ACGATGAGGA CCTGGAGGAC CTGGAGGACC TGGAAGATGA GGAGCTGCCC 
GAGGAGGAGG AGGAGTTGGA GGAGGAGGGG GAGGAGGAAT TGGAGGAAGA GGATTTGGAG 
GAGGAGGAGG AGGTGCCTGA TGACCTGGCC AGCTACACCC AGCAGGAGGA CACCACTGTG 
GCTGAGCCCA AAGAGTTCAA GCGGATCAGC TTCCCAACGG CTGCGCCTCA GAAGGATGAC 
AAGGTTTCAG CTGTGCCTAC AGAGGCCCCC GAGGTGGCCA AAGGCATCCC CAAAGCAGCC 
ACACCCGAGG AGAAGGCAGC TGCAGAGCGT GCGCAGGAAG CCGAGCCCCC CAAGTCTGAG 
GAGAGTTTCA GATCTCGAGA GGAGGAAGAG GGCCAGGAAG GGCAGGATGC CATGTCCAGA 
GCCAAAGCCA ACTGGTTGCG AGCCTTCAAC AAGGTGCGCA TGCAGCTGCA GGAGGCCCGA 
GGAGAAGGGG ATATGTCTAA GTCTCTATGG TTCAAAGGCG GTCCTGGTGG TGGCCTTATC 
ATCATTGACA GCATGCCAGA CATCAGGAAA CGGAAGCCCA TTCCCCTCGT GAGCGACCTG 
GCTATGTCAT TGGTGCAGTC TCGGAAGGCA GGCATCACCT CGGCCTTGGC CTCCAGCACG 
TTGAACAATG AAGAGCTGAA AAATCACGTT TACAAGAAGA CCCTGCAAGC CTTAATCTAC 
CCCATCTCCT GCACCACGCC GCACAATTTC GAGGTGTGGA CGGCCACCAC GCCCACCTAC 
TGCTATGAGT GCGAGGGGCT GCTGTGGGGC ATCGCGCGGC AGGGCATGCG ATGCACCGAG 
TGCGGCGTTA AGTGCCACGA GAAGTGCCAG GACCTGCTCA ATGCGGATTG CCTGCAGCGG 
GCGGCTGAGA AGAGTTCCAA GCATGGCGCT GAAGACCGCA CGCAGAACAT CATCATGGTG 
CTGAAGGACC GCATGAAGAT CCGCGAGCGC AACAAGCCTG AGATCTTCGA GCTTATCCAA 
GAGATCTTCG CGGTCACCAA GAGCGCACAC ACACAGCAGA TGAAAGCTGT CAAGCAGAGT 
GTGCTGGACG GCACATCCAA GTGGTCTGCC AAAATTAGCA TCACAGTGGT CTGTGCCCAG 
GGCTTGCAGG CCAAGGACAA GACAGGATCC AGTGACCCTT ATGTCACCGT CCAGGTTGGG 
AAGACCAAGA AAAGGACAAA AACCATCTAC GGGAACCTCA ACCCAGTGTG GGAAGAGAAT 
TTTCATTTTG AATGTCACAA CTCCTCTGAC CGGATCAAGG TTCGTGTTTG GGATGAAGAT 
GACGACATAA AATCCCGTGT GAAACAAAGG TTTAAGAGGG AGTCTGATGA CTTCCTAGGG 
CAGACAATCA TCGAGGTGCG GACGCTTAGC GGCGAGATGG ATGTGTGGTA CAATCTGGAC 
AAGAGAACGG ACAAGTCGGC GGTGTCGGGG GCCATCCGGC TTCACATCAG TGTGGAGATC 
AAAGGGGAGG AGAAGGTGGC GCCCTACCAT GTCCAGTACA CCTGTCTGCA TGAGAACCTG 
TTCCACTTTG TGACGGACGT GCAGAACAAT GGTGTGGTGA AGATTCCCGA TGCCAAGGGT 
GACGACGCCT GGAAGGTTTA CTACGATGAG ACGGCCCAGG AGATCGTGGA TGAGTTTGCC 
ATGCGCTATG GTGTTGAGTC CATCTACCAA GCCATGACCC ACTTTGCCTG CCTCTCCTCC 
AAGTATATGT GCCCTGGGGT ACCCGCTGTC ATGAGCACCC TGCTTGCCAA CATCAACGCC 
TACTACGCAC ACACCACCGC CTCCACCAAC GTGTCTGCCT CTGACCGCTT CGCTGCCTCT 
AATTTCGGGA AAGAGCGCTT TGTGAAACTT CTGGACCAGC TACACAATTC TCTGCGGATC 
GACCTGTCCA TGTACCGGAA CAACTTCCCA GCCAGCAGCC CTGAGCGGCT GCAGGATCTC 
AAGTCCACGG TGGACCTGCT CACCAGCATC ACCTTCTTCC GGATGAAGGT TCAGGAACTG 
CAGAGCCCGC CGCGTGCCAG CCAGGTAGTG AAAGACTGTG TGAAGGCCTG CCTCAACTCC 
ACCTATGAGT ACATCTTCAA CAACTGCCAT GAGCTCTATG GCCGGGAGTA CCAAACGGAT 
CCGGCCAAGA AGGGGGAGGT TCCCCCAGAG GAGCAAGGCC CTAGCATCAA GAACCTGGAT 
TTCTGGTCCA AGCTGATCAC CCTCATCGTG TCTATCATCG AGGAGGATAA GAATTCTTAC 
ACACCCTGCC TCAATCAGTT TCCCCAGGAG CTCAATGTGG GGAAGATCAG TGCTGAGGTG 
ATGTGGAGCC TATTTGCCCA AGACATGAAG TACGCCATGG AGGAACATGA CAAACACCGG 
CTGTGTAAGA GCGCAGATTA CATGAACCTG CACTTCAAGG TGAAGTGGCT GTACAATGAG 
TACGTGGCCG AGCTGCCCAC CTTCAAGGAC CGTGTGCCTG AGTACCCTGC GTGGTTTGAG 
CCCTTCGTCA TCCAGTGGTT GGATGAGAAT GAGGAGGTGT CCCGGGACTT CCTGCATGGT 
GCCCTCGAGC GGGACAAGAA GGACGGGTTC CAGCAGACCT CAGAGCACGC CCTGTTCTCT 
TGCTCAGTGG TGGACGTCTT CTCCCAGCTT AACCAGAGTT TCGAGATCAT CAAGAAGCTG 
GAGTGTCCTG ACCCCCAGAT CGTGGGCCAT TACATGCGGC GTTTTGCCAA GACCATTAGC 
AATGTGCTTC TCCAGTATGC AGACATCGTT TCCAAGGACT TCGCCTCCTA CTGCTCCAAG 
GAGAAGGAGA AAGTGCCCTG CATCCTCATG AACAACACAC AGCAACTACG AGTGCAGCTG 
GAGAAGATGT TTGAGGCGAT GGGCGGGAAG GAGCTGGATG CCGAGGCCAG TGGCACCCTG 
AAGGAGCTGC AGGTGAAACT CAACAATGTC CTGGATGAAC TCAGCCATGT GTTTGCCACC 
AGCTTCCAGC CACACATTGA GGAATGCGTC AGACAGATGG GTGACATCCT GAGCCAGGTG 
AAGGGCACAG GCAATGTACC TGCCAGTGCC TGCAGCAGCG TGGCCCAGGA TGCAGACAAT 
GTGCTGCAGC CCATCATGGA TCTTCTGGAC AGCAACCTCA CCCTGTTTGC CAAAATCTGT 
GAGAAGACAG TTCTGAAGCG GGTGCTGAAG GAGCTCTGGA AGCTGGTGAT GAACACAATG 
GAGAAGACCA TCGTCCTGCC ACCGCTCACT GACCAGACGA TGATTGGCAC CCTCTTGAGA 
AAACATGGCA AGGGCCTAGA AAAGGGCAGG GTGAAACTGC CAAGCCACTC AGACGGGACA 
CAAATGATCT TCAATGCTGC CAAGGAGCTG GGCCAGCTGT CCAAACTTAA GGATCACATG 
GTGCGGGAAG AAGCCAAGAG CTTGACCCCG AAGCAGTGTG CTGTTGTTGA ACTGGCCCTG 
GACACCATCA AGCAATACTT CCACGCGGGG GGCGTTGGCC TCAAGAAGAC CTTCCTGGAG 
AAGAGCCCGG ACCTCCAGTC CCTGCGCTAC GCCCTGTCGC TCTACACGCA AGCCACCGAC 
CTGCTCATCA AAACCTTCGT GCAGACGCAG TCAGCGCAGG TCCATGGTGG AAAAGGGACT 
AGGTTTACCC TTAGTGAAGA CGTTTGTCCT GAGATGGGCT CTGGTGTGGA GGACCCTGTA 
GGTGAAGTGT CCGTCCACGT GGAGCTGTTC ACACATCCAG GAACTGGGGA ACAGAAGGTC 
ACAGTGAAGG TGGTGGCCGC CAATGACCTC AAGTGGCAGA CTTCTGGCAT CTTCCGCCCG 
TTCATCGAGG TCAACATCGT TGGACCTCAG CTTAGCGACA AGAAACGCAA GTTCGCCACC 
AAATCCAAAA ACAACAGCTG GGCGCCCAAG TATAACGAGA GCTTCCAGTT CTCCCTGAGC 
GCCGACGCGG GCCCCGAGTG CTACGAGCTG CAGGTGTGCG TGAAGGACTA CTGCTTTGCG 
CGCGAAGACC GCACGGTGGG GCTGGCGGTG CTGCAACTGC GAGAGCTGGC CCAGCGCGGG 
AGCGCCGCGT GCTGGCTTCC ACTCGGCCGC CGCATCCACA TGGACGACAC GGGGCTCACA 
GTGCTGCGCA TCCTGTCGCA GCGCAGCAAC GATGAGGTGG CCAAGGAATT CGTCAAGCTC 
AAGTCGGACA CGCGCTCAGC CGAGGAGGGC GGTGCCGCGC CTGCGCCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Unc13a ( XM_017312875.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168364.1 Mus musculus unc-13 homolog A (Unc13a), transcript variant X2, mRNA.

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CloneID	OMu82526
Clone ID Related Accession (Same CDS sequence)	XM_017312875.1 , XM_017312875.2
Accession Version	XM_017312875.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein unc-13 homolog A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017312875.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTGC TGTGCGTGGG AGTCAAAAAA GCCAAGTTTG ACGGTGCCCA AGAGAAGTTC 
AACACATACG TGACGCTGAA GGTGCAGAAC GTGAAGAGCA CTACCATAGC TGTACGTGGC 
AGCCAGCCCA GCTGGGAGCA GGACTTCATG TTTGAGATCA ACCGCCTGGA TCTAGGCCTG 
ACCGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGTAC TGTGTGGATC 
CCGCTGCGGA CCATCCGCCA GTCCAATGAG GAGGGCCCAG GAGAGTGGCT CACACTGGAC 
TCTCAGGCCA TCATGGCGGA CAGTGAGATC TGTGGGACCA AGGACCCCAC CTTCCATCGC 
ATCCTCCTGG ACGCTCATTT CGAGCTGCCG TTGGACATCC CTGAGGAGGA GGCACGCTAC 
TGGGCCAAGA AGCTGGAGCA GCTGAATGCC ATGCGTGACC AGGATGAGGT GAGTCACCTC 
CAGTACTCCT TCCAGGACCA GCAGGACAAG CCGCTGCCAG TGCCCAGCAG CCAGTGCTGC 
AACTGGAATT ACTTTGGCTG GGGAGAACAG AATGACGACC CCGACAGTGC GGTGGATGAC 
CGGGACAGTG ACTATAGGAG TGAGACCAGC AACAGCATCC CACCGCCCTA CTATACGACT 
TCGCAGCCCA ACGCCTCTGT GCACCAGTAC TCCGTGCGAC CGCCCCCTCT GGGGTCCCGG 
GAGTCCTACA GTGACTCCAT GCACAGCTAT GAGGAGTTCT CTGAGCCGCG GGCGCTCAGC 
CCCACAGGCA GCAGCCGCTA TGCTTCTTCC GGGGAGCTGA GCCAGGGAAG CTCACAGCTG 
AGCGAGGACT TCGACCCTGA TGAGCACAGC CTGCAGGGCT CGGAGCTGGA TGACGAGAGG 
GACCGCGATT CTTATCACTC CTGTCACAGC TCCGTGAGTT ACCACAAGGA CTCACCCCGC 
TGGGACCAGG ACGATGAGGA CCTGGAGGAC CTGGAGGACC TGGAAGATGA GGAGCTGCCC 
GAGGAGGAGG AGGAGTTGGA GGAGGAGGGG GAGGAGGAAT TGGAGGAAGA GGATTTGGAG 
GAGGAGGAGG AGGTGCCTGA TGACCTGGCC AGCTACACCC AGCAGGAGGA CACCACTGTG 
GCTGAGCCCA AAGAGTTCAA GCGGATCAGC TTCCCAACGG CTGCGCCTCA GAAGGATGAC 
AAGGTTTCAG CTGTGCCTAC AGAGGCCCCC GAGGTGGCCA AAGGCATCCC CAAAGCAGCC 
ACACCCGAGG AGAAGGCAGC TGCAGAGCGT GCGCAGGAAG CCGAGCCCCC CAAGTCTGAG 
GAGAGTTTCA GATCTCGAGA GGAGGAAGAG GGCCAGGAAG GGCAGGATGC CATGTCCAGA 
GCCAAAGCCA ACTGGTTGCG AGCCTTCAAC AAGGTGCGCA TGCAGCTGCA GGAGGCCCGA 
GGAGAAGGGG ATATGTCTAA GTCTCTATGG TTCAAAGGCG GTCCTGGTGG TGGCCTTATC 
ATCATTGACA GCATGCCAGA CATCAGGAAA CGGAAGCCCA TTCCCCTCGT GAGCGACCTG 
GCTATGTCAT TGGTGCAGTC TCGGAAGGCA GGCATCACCT CGGCCTTGGC CTCCAGCACG 
TTGAACAATG AAGAGCTGAA AAATCACGTT TACAAGAAGA CCCTGCAAGC CTTAATCTAC 
CCCATCTCCT GCACCACGCC GCACAATTTC GAGGTGTGGA CGGCCACCAC GCCCACCTAC 
TGCTATGAGT GCGAGGGGCT GCTGTGGGGC ATCGCGCGGC AGGGCATGCG ATGCACCGAG 
TGCGGCGTTA AGTGCCACGA GAAGTGCCAG GACCTGCTCA ATGCGGATTG CCTGCAGCGG 
GCGGCTGAGA AGAGTTCCAA GCATGGCGCT GAAGACCGCA CGCAGAACAT CATCATGGTG 
CTGAAGGACC GCATGAAGAT CCGCGAGCGC AACAAGCCTG AGATCTTCGA GCTTATCCAA 
GAGATCTTCG CGGTCACCAA GAGCGCACAC ACACAGCAGA TGAAAGCTGT CAAGCAGAGT 
GTGCTGGACG GCACATCCAA GTGGTCTGCC AAAATTAGCA TCACAGTGGT CTGTGCCCAG 
GGCTTGCAGG CCAAGGACAA GACAGGATCC AGTGACCCTT ATGTCACCGT CCAGGTTGGG 
AAGACCAAGA AAAGGACAAA AACCATCTAC GGGAACCTCA ACCCAGTGTG GGAAGAGAAT 
TTTCATTTTG AATGTCACAA CTCCTCTGAC CGGATCAAGG TTCGTGTTTG GGATGAAGAT 
GACGACATAA AATCCCGTGT GAAACAAAGG TTTAAGAGGG AGTCTGATGA CTTCCTAGGG 
CAGACAATCA TCGAGGTGCG GACGCTTAGC GGCGAGATGG ATGTGTGGTA CAATCTGGAC 
AAGAGAACGG ACAAGTCGGC GGTGTCGGGG GCCATCCGGC TTCACATCAG TGTGGAGATC 
AAAGGGGAGG AGAAGGTGGC GCCCTACCAT GTCCAGTACA CCTGTCTGCA TGAGAACCTG 
TTCCACTTTG TGACGGACGT GCAGAACAAT GGTGTGGTGA AGATTCCCGA TGCCAAGGGT 
GACGACGCCT GGAAGGTTTA CTACGATGAG ACGGCCCAGG AGATCGTGGA TGAGTTTGCC 
ATGCGCTATG GTGTTGAGTC CATCTACCAA GCCATGACCC ACTTTGCCTG CCTCTCCTCC 
AAGTATATGT GCCCTGGGGT ACCCGCTGTC ATGAGCACCC TGCTTGCCAA CATCAACGCC 
TACTACGCAC ACACCACCGC CTCCACCAAC GTGTCTGCCT CTGACCGCTT CGCTGCCTCT 
AATTTCGGGA AAGAGCGCTT TGTGAAACTT CTGGACCAGC TACACAATTC TCTGCGGATC 
GACCTGTCCA TGTACCGGAA CAACTTCCCA GCCAGCAGCC CTGAGCGGCT GCAGGATCTC 
AAGTCCACGG TGGACCTGCT CACCAGCATC ACCTTCTTCC GGATGAAGGT TCAGGAACTG 
CAGAGCCCGC CGCGTGCCAG CCAGGTAGTG AAAGACTGTG TGAAGGCCTG CCTCAACTCC 
ACCTATGAGT ACATCTTCAA CAACTGCCAT GAGCTCTATG GCCGGGAGTA CCAAACGGAT 
CCGGCCAAGA AGGGGGAGGT TCCCCCAGAG GAGCAAGGCC CTAGCATCAA GAACCTGGAT 
TTCTGGTCCA AGCTGATCAC CCTCATCGTG TCTATCATCG AGGAGGATAA GAATTCTTAC 
ACACCCTGCC TCAATCAGTT TCCCCAGGAG CTCAATGTGG GGAAGATCAG TGCTGAGGTG 
ATGTGGAGCC TATTTGCCCA AGACATGAAG TACGCCATGG AGGAACATGA CAAACACCGG 
CTGTGTAAGA GCGCAGATTA CATGAACCTG CACTTCAAGG TGAAGTGGCT GTACAATGAG 
TACGTGGCCG AGCTGCCCAC CTTCAAGGAC CGTGTGCCTG AGTACCCTGC GTGGTTTGAG 
CCCTTCGTCA TCCAGTGGTT GGATGAGAAT GAGGAGGTGT CCCGGGACTT CCTGCATGGT 
GCCCTCGAGC GGGACAAGAA GGACGGGTTC CAGCAGACCT CAGAGCACGC CCTGTTCTCT 
TGCTCAGTGG TGGACGTCTT CTCCCAGCTT AACCAGAGTT TCGAGATCAT CAAGAAGCTG 
GAGTGTCCTG ACCCCCAGAT CGTGGGCCAT TACATGCGGC GTTTTGCCAA GACCATTAGC 
AATGTGCTTC TCCAGTATGC AGACATCGTT TCCAAGGACT TCGCCTCCTA CTGCTCCAAG 
GAGAAGGAGA AACCCTGCAT CCTCATGAAC AACACACAGC AACTACGAGT GCAGCTGGAG 
AAGATGTTTG AGGCGATGGG CGGGAAGGAG CTGGATGCCG AGGCCAGTGG CACCCTGAAG 
GAGCTGCAGG TGAAACTCAA CAATGTCCTG GATGAACTCA GCCATGTGTT TGCCACCAGC 
TTCCAGCCAC ACATTGAGGA ATGCGTCAGA CAGATGGGTG ACATCCTGAG CCAGGTGAAG 
GGCACAGGCA ATGTACCTGC CAGTGCCTGC AGCAGCGTGG CCCAGGATGC AGACAATGTG 
CTGCAGCCCA TCATGGATCT TCTGGACAGC AACCTCACCC TGTTTGCCAA AATCTGTGAG 
AAGACAGTTC TGAAGCGGGT GCTGAAGGAG CTCTGGAAGC TGGTGATGAA CACAATGGAG 
AAGACCATCG TCCTGCCACC GCTCACTGAC CAGACGATGA TTGGCACCCT CTTGAGAAAA 
CATGGCAAGG GCCTAGAAAA GGGCAGGGTG AAACTGCCAA GCCACTCAGA CGGGACACAA 
ATGATCTTCA ATGCTGCCAA GGAGCTGGGC CAGCTGTCCA AACTTAAGGA TCACATGGTG 
CGGGAAGAAG CCAAGAGCTT GACCCCGAAG CAGTGTGCTG TTGTTGAACT GGCCCTGGAC 
ACCATCAAGC AATACTTCCA CGCGGGGGGC GTTGGCCTCA AGAAGACCTT CCTGGAGAAG 
AGCCCGGACC TCCAGTCCCT GCGCTACGCC CTGTCGCTCT ACACGCAAGC CACCGACCTG 
CTCATCAAAA CCTTCGTGCA GACGCAGTCA GCGCAGGTCC ATGGTGGAAA AGGGACTAGG 
TTTACCCTTA GTGAAGACGT TTGTCCTGAG ATGGGCTCTG GTGTGGAGGA CCCTGTAGGT 
GAAGTGTCCG TCCACGTGGA GCTGTTCACA CATCCAGGAA CTGGGGAACA GAAGGTCACA 
GTGAAGGTGG TGGCCGCCAA TGACCTCAAG TGGCAGACTT CTGGCATCTT CCGCCCGTTC 
ATCGAGGTCA ACATCGTTGG ACCTCAGCTT AGCGACAAGA AACGCAAGTT CGCCACCAAA 
TCCAAAAACA ACAGCTGGGC GCCCAAGTAT AACGAGAGCT TCCAGTTCTC CCTGAGCGCC 
GACGCGGGCC CCGAGTGCTA CGAGCTGCAG GTGTGCGTGA AGGACTACTG CTTTGCGCGC 
GAAGACCGCA CGGTGGGGCT GGCGGTGCTG CAACTGCGAG AGCTGGCCCA GCGCGGGAGC 
GCCGCGTGCT GGCTTCCACT CGGCCGCCGC ATCCACATGG ACGACACGGG GCTCACAGTG 
CTGCGCATCC TGTCGCAGCG CAGCAACGAT GAGGTGGCCA AGGAATTCGT CAAGCTCAAG 
TCGGACACGC GCTCAGCCGA GGAGGGCGGT GCCGCGCCTG CGCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168364.1
CDS	127..5334
Translation	MSLLCVGVKKAKFDGAQEKFNTYVTLKVQNVKSTTIAVRGSQPSWEQDFMFEINRLDLGLTVEVWNKGLIWDTMVGTVWIPLRTIRQSNEEGPGEWLTLDSQAIMADSEICGTKDPTFHRILLDAHFELPLDIPEEEARYWAKKLEQLNAMRDQDEVSHLQYSFQDQQDKPLPVPSSQCCNWNYFGWGEQNDDPDSAVDDRDSDYRSETSNSIPPPYYTTSQPNASVHQYSVRPPPLGSRESYSDSMHSYEEFSEPRALSPTGSSRYASSGELSQGSSQLSEDFDPDEHSLQGSELDDERDRDSYHSCHSSVSYHKDSPRWDQDDEDLEDLEDLEDEELPEEEEELEEEGEEELEEEDLEEEEEVPDDLASYTQQEDTTVAEPKEFKRISFPTAAPQKDDKVSAVPTEAPEVAKGIPKAATPEEKAAAERAQEAEPPKSEESFRSREEEEGQEGQDAMSRAKANWLRAFNKVRMQLQEARGEGDMSKSLWFKGGPGGGLIIIDSMPDIRKRKPIPLVSDLAMSLVQSRKAGITSALASSTLNNEELKNHVYKKTLQALIYPISCTTPHNFEVWTATTPTYCYECEGLLWGIARQGMRCTECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMVLKDRMKIRERNKPEIFELIQEIFAVTKSAHTQQMKAVKQSVLDGTSKWSAKISITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIYGNLNPVWEENFHFECHNSSDRIKVRVWDEDDDIKSRVKQRFKRESDDFLGQTIIEVRTLSGEMDVWYNLDKRTDKSAVSGAIRLHISVEIKGEEKVAPYHVQYTCLHENLFHFVTDVQNNGVVKIPDAKGDDAWKVYYDETAQEIVDEFAMRYGVESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSMYRNNFPASSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHELYGREYQTDPAKKGEVPPEEQGPSIKNLDFWSKLITLIVSIIEEDKNSYTPCLNQFPQELNVGKISAEVMWSLFAQDMKYAMEEHDKHRLCKSADYMNLHFKVKWLYNEYVAELPTFKDRVPEYPAWFEPFVIQWLDENEEVSRDFLHGALERDKKDGFQQTSEHALFSCSVVDVFSQLNQSFEIIKKLECPDPQIVGHYMRRFAKTISNVLLQYADIVSKDFASYCSKEKEKPCILMNNTQQLRVQLEKMFEAMGGKELDAEASGTLKELQVKLNNVLDELSHVFATSFQPHIEECVRQMGDILSQVKGTGNVPASACSSVAQDADNVLQPIMDLLDSNLTLFAKICEKTVLKRVLKELWKLVMNTMEKTIVLPPLTDQTMIGTLLRKHGKGLEKGRVKLPSHSDGTQMIFNAAKELGQLSKLKDHMVREEAKSLTPKQCAVVELALDTIKQYFHAGGVGLKKTFLEKSPDLQSLRYALSLYTQATDLLIKTFVQTQSAQVHGGKGTRFTLSEDVCPEMGSGVEDPVGEVSVHVELFTHPGTGEQKVTVKVVAANDLKWQTSGIFRPFIEVNIVGPQLSDKKRKFATKSKNNSWAPKYNESFQFSLSADAGPECYELQVCVKDYCFAREDRTVGLAVLQLRELAQRGSAACWLPLGRRIHMDDTGLTVLRILSQRSNDEVAKEFVKLKSDTRSAEEGGAAPAP

Target ORF information:

RefSeq Version	XM_017312875.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus unc-13 homolog A (Unc13a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312875.2

ORF Insert Sequence:

ATGTCTCTGC TGTGCGTGGG AGTCAAAAAA GCCAAGTTTG ACGGTGCCCA AGAGAAGTTC 
AACACATACG TGACGCTGAA GGTGCAGAAC GTGAAGAGCA CTACCATAGC TGTACGTGGC 
AGCCAGCCCA GCTGGGAGCA GGACTTCATG TTTGAGATCA ACCGCCTGGA TCTAGGCCTG 
ACCGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGTAC TGTGTGGATC 
CCGCTGCGGA CCATCCGCCA GTCCAATGAG GAGGGCCCAG GAGAGTGGCT CACACTGGAC 
TCTCAGGCCA TCATGGCGGA CAGTGAGATC TGTGGGACCA AGGACCCCAC CTTCCATCGC 
ATCCTCCTGG ACGCTCATTT CGAGCTGCCG TTGGACATCC CTGAGGAGGA GGCACGCTAC 
TGGGCCAAGA AGCTGGAGCA GCTGAATGCC ATGCGTGACC AGGATGAGGT GAGTCACCTC 
CAGTACTCCT TCCAGGACCA GCAGGACAAG CCGCTGCCAG TGCCCAGCAG CCAGTGCTGC 
AACTGGAATT ACTTTGGCTG GGGAGAACAG AATGACGACC CCGACAGTGC GGTGGATGAC 
CGGGACAGTG ACTATAGGAG TGAGACCAGC AACAGCATCC CACCGCCCTA CTATACGACT 
TCGCAGCCCA ACGCCTCTGT GCACCAGTAC TCCGTGCGAC CGCCCCCTCT GGGGTCCCGG 
GAGTCCTACA GTGACTCCAT GCACAGCTAT GAGGAGTTCT CTGAGCCGCG GGCGCTCAGC 
CCCACAGGCA GCAGCCGCTA TGCTTCTTCC GGGGAGCTGA GCCAGGGAAG CTCACAGCTG 
AGCGAGGACT TCGACCCTGA TGAGCACAGC CTGCAGGGCT CGGAGCTGGA TGACGAGAGG 
GACCGCGATT CTTATCACTC CTGTCACAGC TCCGTGAGTT ACCACAAGGA CTCACCCCGC 
TGGGACCAGG ACGATGAGGA CCTGGAGGAC CTGGAGGACC TGGAAGATGA GGAGCTGCCC 
GAGGAGGAGG AGGAGTTGGA GGAGGAGGGG GAGGAGGAAT TGGAGGAAGA GGATTTGGAG 
GAGGAGGAGG AGGTGCCTGA TGACCTGGCC AGCTACACCC AGCAGGAGGA CACCACTGTG 
GCTGAGCCCA AAGAGTTCAA GCGGATCAGC TTCCCAACGG CTGCGCCTCA GAAGGATGAC 
AAGGTTTCAG CTGTGCCTAC AGAGGCCCCC GAGGTGGCCA AAGGCATCCC CAAAGCAGCC 
ACACCCGAGG AGAAGGCAGC TGCAGAGCGT GCGCAGGAAG CCGAGCCCCC CAAGTCTGAG 
GAGAGTTTCA GATCTCGAGA GGAGGAAGAG GGCCAGGAAG GGCAGGATGC CATGTCCAGA 
GCCAAAGCCA ACTGGTTGCG AGCCTTCAAC AAGGTGCGCA TGCAGCTGCA GGAGGCCCGA 
GGAGAAGGGG ATATGTCTAA GTCTCTATGG TTCAAAGGCG GTCCTGGTGG TGGCCTTATC 
ATCATTGACA GCATGCCAGA CATCAGGAAA CGGAAGCCCA TTCCCCTCGT GAGCGACCTG 
GCTATGTCAT TGGTGCAGTC TCGGAAGGCA GGCATCACCT CGGCCTTGGC CTCCAGCACG 
TTGAACAATG AAGAGCTGAA AAATCACGTT TACAAGAAGA CCCTGCAAGC CTTAATCTAC 
CCCATCTCCT GCACCACGCC GCACAATTTC GAGGTGTGGA CGGCCACCAC GCCCACCTAC 
TGCTATGAGT GCGAGGGGCT GCTGTGGGGC ATCGCGCGGC AGGGCATGCG ATGCACCGAG 
TGCGGCGTTA AGTGCCACGA GAAGTGCCAG GACCTGCTCA ATGCGGATTG CCTGCAGCGG 
GCGGCTGAGA AGAGTTCCAA GCATGGCGCT GAAGACCGCA CGCAGAACAT CATCATGGTG 
CTGAAGGACC GCATGAAGAT CCGCGAGCGC AACAAGCCTG AGATCTTCGA GCTTATCCAA 
GAGATCTTCG CGGTCACCAA GAGCGCACAC ACACAGCAGA TGAAAGCTGT CAAGCAGAGT 
GTGCTGGACG GCACATCCAA GTGGTCTGCC AAAATTAGCA TCACAGTGGT CTGTGCCCAG 
GGCTTGCAGG CCAAGGACAA GACAGGATCC AGTGACCCTT ATGTCACCGT CCAGGTTGGG 
AAGACCAAGA AAAGGACAAA AACCATCTAC GGGAACCTCA ACCCAGTGTG GGAAGAGAAT 
TTTCATTTTG AATGTCACAA CTCCTCTGAC CGGATCAAGG TTCGTGTTTG GGATGAAGAT 
GACGACATAA AATCCCGTGT GAAACAAAGG TTTAAGAGGG AGTCTGATGA CTTCCTAGGG 
CAGACAATCA TCGAGGTGCG GACGCTTAGC GGCGAGATGG ATGTGTGGTA CAATCTGGAC 
AAGAGAACGG ACAAGTCGGC GGTGTCGGGG GCCATCCGGC TTCACATCAG TGTGGAGATC 
AAAGGGGAGG AGAAGGTGGC GCCCTACCAT GTCCAGTACA CCTGTCTGCA TGAGAACCTG 
TTCCACTTTG TGACGGACGT GCAGAACAAT GGTGTGGTGA AGATTCCCGA TGCCAAGGGT 
GACGACGCCT GGAAGGTTTA CTACGATGAG ACGGCCCAGG AGATCGTGGA TGAGTTTGCC 
ATGCGCTATG GTGTTGAGTC CATCTACCAA GCCATGACCC ACTTTGCCTG CCTCTCCTCC 
AAGTATATGT GCCCTGGGGT ACCCGCTGTC ATGAGCACCC TGCTTGCCAA CATCAACGCC 
TACTACGCAC ACACCACCGC CTCCACCAAC GTGTCTGCCT CTGACCGCTT CGCTGCCTCT 
AATTTCGGGA AAGAGCGCTT TGTGAAACTT CTGGACCAGC TACACAATTC TCTGCGGATC 
GACCTGTCCA TGTACCGGAA CAACTTCCCA GCCAGCAGCC CTGAGCGGCT GCAGGATCTC 
AAGTCCACGG TGGACCTGCT CACCAGCATC ACCTTCTTCC GGATGAAGGT TCAGGAACTG 
CAGAGCCCGC CGCGTGCCAG CCAGGTAGTG AAAGACTGTG TGAAGGCCTG CCTCAACTCC 
ACCTATGAGT ACATCTTCAA CAACTGCCAT GAGCTCTATG GCCGGGAGTA CCAAACGGAT 
CCGGCCAAGA AGGGGGAGGT TCCCCCAGAG GAGCAAGGCC CTAGCATCAA GAACCTGGAT 
TTCTGGTCCA AGCTGATCAC CCTCATCGTG TCTATCATCG AGGAGGATAA GAATTCTTAC 
ACACCCTGCC TCAATCAGTT TCCCCAGGAG CTCAATGTGG GGAAGATCAG TGCTGAGGTG 
ATGTGGAGCC TATTTGCCCA AGACATGAAG TACGCCATGG AGGAACATGA CAAACACCGG 
CTGTGTAAGA GCGCAGATTA CATGAACCTG CACTTCAAGG TGAAGTGGCT GTACAATGAG 
TACGTGGCCG AGCTGCCCAC CTTCAAGGAC CGTGTGCCTG AGTACCCTGC GTGGTTTGAG 
CCCTTCGTCA TCCAGTGGTT GGATGAGAAT GAGGAGGTGT CCCGGGACTT CCTGCATGGT 
GCCCTCGAGC GGGACAAGAA GGACGGGTTC CAGCAGACCT CAGAGCACGC CCTGTTCTCT 
TGCTCAGTGG TGGACGTCTT CTCCCAGCTT AACCAGAGTT TCGAGATCAT CAAGAAGCTG 
GAGTGTCCTG ACCCCCAGAT CGTGGGCCAT TACATGCGGC GTTTTGCCAA GACCATTAGC 
AATGTGCTTC TCCAGTATGC AGACATCGTT TCCAAGGACT TCGCCTCCTA CTGCTCCAAG 
GAGAAGGAGA AACCCTGCAT CCTCATGAAC AACACACAGC AACTACGAGT GCAGCTGGAG 
AAGATGTTTG AGGCGATGGG CGGGAAGGAG CTGGATGCCG AGGCCAGTGG CACCCTGAAG 
GAGCTGCAGG TGAAACTCAA CAATGTCCTG GATGAACTCA GCCATGTGTT TGCCACCAGC 
TTCCAGCCAC ACATTGAGGA ATGCGTCAGA CAGATGGGTG ACATCCTGAG CCAGGTGAAG 
GGCACAGGCA ATGTACCTGC CAGTGCCTGC AGCAGCGTGG CCCAGGATGC AGACAATGTG 
CTGCAGCCCA TCATGGATCT TCTGGACAGC AACCTCACCC TGTTTGCCAA AATCTGTGAG 
AAGACAGTTC TGAAGCGGGT GCTGAAGGAG CTCTGGAAGC TGGTGATGAA CACAATGGAG 
AAGACCATCG TCCTGCCACC GCTCACTGAC CAGACGATGA TTGGCACCCT CTTGAGAAAA 
CATGGCAAGG GCCTAGAAAA GGGCAGGGTG AAACTGCCAA GCCACTCAGA CGGGACACAA 
ATGATCTTCA ATGCTGCCAA GGAGCTGGGC CAGCTGTCCA AACTTAAGGA TCACATGGTG 
CGGGAAGAAG CCAAGAGCTT GACCCCGAAG CAGTGTGCTG TTGTTGAACT GGCCCTGGAC 
ACCATCAAGC AATACTTCCA CGCGGGGGGC GTTGGCCTCA AGAAGACCTT CCTGGAGAAG 
AGCCCGGACC TCCAGTCCCT GCGCTACGCC CTGTCGCTCT ACACGCAAGC CACCGACCTG 
CTCATCAAAA CCTTCGTGCA GACGCAGTCA GCGCAGGTCC ATGGTGGAAA AGGGACTAGG 
TTTACCCTTA GTGAAGACGT TTGTCCTGAG ATGGGCTCTG GTGTGGAGGA CCCTGTAGGT 
GAAGTGTCCG TCCACGTGGA GCTGTTCACA CATCCAGGAA CTGGGGAACA GAAGGTCACA 
GTGAAGGTGG TGGCCGCCAA TGACCTCAAG TGGCAGACTT CTGGCATCTT CCGCCCGTTC 
ATCGAGGTCA ACATCGTTGG ACCTCAGCTT AGCGACAAGA AACGCAAGTT CGCCACCAAA 
TCCAAAAACA ACAGCTGGGC GCCCAAGTAT AACGAGAGCT TCCAGTTCTC CCTGAGCGCC 
GACGCGGGCC CCGAGTGCTA CGAGCTGCAG GTGTGCGTGA AGGACTACTG CTTTGCGCGC 
GAAGACCGCA CGGTGGGGCT GGCGGTGCTG CAACTGCGAG AGCTGGCCCA GCGCGGGAGC 
GCCGCGTGCT GGCTTCCACT CGGCCGCCGC ATCCACATGG ACGACACGGG GCTCACAGTG 
CTGCGCATCC TGTCGCAGCG CAGCAACGAT GAGGTGGCCA AGGAATTCGT CAAGCTCAAG 
TCGGACACGC GCTCAGCCGA GGAGGGCGGT GCCGCGCCTG CGCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Unc13a ( XM_017312877.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168366.1 Mus musculus unc-13 homolog A (Unc13a), transcript variant X6, mRNA.

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CloneID	OMu82527
Clone ID Related Accession (Same CDS sequence)	XM_017312877.1 , XM_017312877.2
Accession Version	XM_017312877.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5154bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein unc-13 homolog A isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017312877.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTGC TGTGCGTGGG AGTCAAAAAA GCCAAGTTTG ACGGTGCCCA AGAGAAGTTC 
AACACATACG TGACGCTGAA GGTGCAGAAC GTGAAGAGCA CTACCATAGC TGTACGTGGC 
AGCCAGCCCA GCTGGGAGCA GGACTTCATG TTTGAGATCA ACCGCCTGGA TCTAGGCCTG 
ACCGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGTAC TGTGTGGATC 
CCGCTGCGGA CCATCCGCCA GTCCAATGAG GAGGGCCCAG GAGAGTGGCT CACACTGGAC 
TCTCAGGCCA TCATGGCGGA CAGTGAGATC TGTGGGACCA AGGACCCCAC CTTCCATCGC 
ATCCTCCTGG ACGCTCATTT CGAGCTGCCG TTGGACATCC CTGAGGAGGA GGCACGCTAC 
TGGGCCAAGA AGCTGGAGCA GCTGAATGCC ATGCGTGACC AGGATGAGGT GAGTCACCTC 
CAGTACTCCT TCCAGGACCA GCAGGACAAG CCGCTGCCAG TGCCCAGCAG CCAGTGCTGC 
AACTGGAATT ACTTTGGCTG GGGAGAACAG AATGACGACC CCGACAGTGC GGTGGATGAC 
CGGGACAGTG ACTATAGGAG TGAGACCAGC AACAGCATCC CACCGCCCTA CTATACGACT 
TCGCAGCCCA ACGCCTCTGT GCACCAGTAC TCCGTGCGAC CGCCCCCTCT GGGGTCCCGG 
GAGTCCTACA GTGACTCCAT GCACAGCTAT GAGGAGTTCT CTGAGCCGCG GGCGCTCAGC 
CCCACAGGCA GCAGCCGCTA TGCTTCTTCC GGGGAGCTGA GCCAGGGAAG CTCACAGCTG 
AGCGAGGACT TCGACCCTGA TGAGCACAGC CTGCAGGGCT CGGAGCTGGA TGACGAGAGG 
GACCGCGATT CTTATCACTC CTGTCACAGC TCCGTGAGTT ACCACAAGGA CTCACCCCGC 
TGGGACCAGG ACGATGAGGA CCTGGAGGAC CTGGAGGACC TGGAAGATGA GGAGCTGCCC 
GAGGAGGAGG AGGAGTTGGA GGAGGAGGGG GAGGAGGAAT TGGAGGAAGA GGATTTGGAG 
GAGGAGGAGG AGGTGCCTGA TGACCTGGCC AGCTACACCC AGCAGGAGGA CACCACTGTG 
GCTGAGCCCA AAGAGTTCAA GCGGATCAGC TTCCCAACGG CTGCGCCTCA GAAGGATGAC 
AAGGTTTCAG CTGTGCCTAC AGAGGCCCCC GAGGTGGCCA AAGGCATCCC CAAAGCAGCC 
ACACCCGAGG AGAAGGCAGC TGCAGAGCGT GCGCAGGAAG CCGAGCCCCC CAAGTCTGAG 
GAGAGTTTCA GATCTCGAGA GGAGGAAGAG GGCCAGGAAG GGCAGGATGC CATGTCCAGA 
GCCAAAGCCA ACTGGTTGCG AGCCTTCAAC AAGGTGCGCA TGCAGCTGCA GGAGGCCCGA 
GGAGAAGGGG ATATGTCTAA GTCTCTATGG TTCAAAGGCG GTCCTGGTGG TGGCCTTATC 
ATCATTGACA GCATGCCAGA CATCAGGAAA CGGAAGCCCA TTCCCCTCGT GAGCGACCTG 
GCTATGTCAT TGGTGCAGTC TCGGAAGGCA GGCATCACCT CGGCCTTGGC CTCCAGCACG 
TTGAACAATG AAGAGCTGAA AAATCACGTT TACAAGAAGA CCCTGCAAGC CTTAATCTAC 
CCCATCTCCT GCACCACGCC GCACAATTTC GAGGTGTGGA CGGCCACCAC GCCCACCTAC 
TGCTATGAGT GCGAGGGGCT GCTGTGGGGC ATCGCGCGGC AGGGCATGCG ATGCACCGAG 
TGCGGCGTTA AGTGCCACGA GAAGTGCCAG GACCTGCTCA ATGCGGATTG CCTGCAGCGG 
GCGGCTGAGA AGAGTTCCAA GCATGGCGCT GAAGACCGCA CGCAGAACAT CATCATGGTG 
CTGAAGGACC GCATGAAGAT CCGCGAGCGC AACAAGCCTG AGATCTTCGA GCTTATCCAA 
GAGATCTTCG CGGTCACCAA GAGCGCACAC ACACAGCAGA TGAAAGCTGT CAAGCAGAGT 
GTGCTGGACG GCACATCCAA GTGGTCTGCC AAAATTAGCA TCACAGTGGT CTGTGCCCAG 
GGCTTGCAGG CCAAGGACAA GACAGGATCC AGTGACCCTT ATGTCACCGT CCAGGTTGGG 
AAGACCAAGA AAAGGACAAA AACCATCTAC GGGAACCTCA ACCCAGTGTG GGAAGAGAAT 
TTTCATTTTG AATGTCACAA CTCCTCTGAC CGGATCAAGG TTCGTGTTTG GGATGAAGAT 
GACGACATAA AATCCCGTGT GAAACAAAGG TTTAAGAGGG AGTCTGATGA CTTCCTAGGG 
CAGACAATCA TCGAGGTGCG GACGCTTAGC GGCGAGATGG ATGTGTGGTA CAATCTGGAC 
AAGAGAACGG ACAAGTCGGC GGTGTCGGGG GCCATCCGGC TTCACATCAG TGTGGAGATC 
AAAGGGGAGG AGAAGGTGGC GCCCTACCAT GTCCAGTACA CCTGTCTGCA TGAGAACCTG 
TTCCACTTTG TGACGGACGT GCAGAACAAT GGTGTGGTGA AGATTCCCGA TGCCAAGGGT 
GACGACGCCT GGAAGGTTTA CTACGATGAG ACGGCCCAGG AGATCGTGGA TGAGTTTGCC 
ATGCGCTATG GTGTTGAGTC CATCTACCAA GCCATGACCC ACTTTGCCTG CCTCTCCTCC 
AAGTATATGT GCCCTGGGGT ACCCGCTGTC ATGAGCACCC TGCTTGCCAA CATCAACGCC 
TACTACGCAC ACACCACCGC CTCCACCAAC GTGTCTGCCT CTGACCGCTT CGCTGCCTCT 
AATTTCGGGA AAGAGCGCTT TGTGAAACTT CTGGACCAGC TACACAATTC TCTGCGGATC 
GACCTGTCCA TGTACCGGAA CAACTTCCCA GCCAGCAGCC CTGAGCGGCT GCAGGATCTC 
AAGTCCACGG TGGACCTGCT CACCAGCATC ACCTTCTTCC GGATGAAGGT TCAGGAACTG 
CAGAGCCCGC CGCGTGCCAG CCAGGTAGTG AAAGACTGTG TGAAGGCCTG CCTCAACTCC 
ACCTATGAGT ACATCTTCAA CAACTGCCAT GAGCTCTATG GCCGGGAGTA CCAAACGGAT 
CCGGCCAAGA AGGGGGAGGT TCCCCCAGAG GAGCAAGGCC CTAGCATCAA GAACCTGGAT 
TTCTGGTCCA AGCTGATCAC CCTCATCGTG TCTATCATCG AGGAGGATAA GAATTCTTAC 
ACACCCTGCC TCAATCAGTT TCCCCAGGAG CTCAATGTGG GGAAGATCAG TGCTGAGGTG 
ATGTGGAGCC TATTTGCCCA AGACATGAAG TACGCCATGG AGGAACATGA CAAACACCGG 
CTGTGTAAGA GCGCAGATTA CATGAACCTG CACTTCAAGG TGAAGTGGCT GTACAATGAG 
TACGTGGCCG AGCTGCCCAC CTTCAAGGAC CGTGTGCCTG AGTACCCTGC GTGGTTTGAG 
CCCTTCGTCA TCCAGTGGTT GGATGAGAAT GAGGAGGTGT CCCGGGACTT CCTGCATGGT 
GCCCTCGAGC GGGACAAGAA GGACGGGTTC CAGCAGACCT CAGAGCACGC CCTGTTCTCT 
TGCTCAGTGG TGGACGTCTT CTCCCAGCTT AACCAGAGTT TCGAGATCAT CAAGAAGCTG 
GAGTGTCCTG ACCCCCAGAT CGTGGGCCAT TACATGCGGC GTTTTGCCAA GACCATTAGC 
AATGTGCTTC TCCAGTATGC AGACATCGTT TCCAAGGACT TCGCCTCCTA CTGCTCCAAG 
GAGAAGGAGA AAGTGCCCTG CATCCTCATG AACAACACAC AGCAACTACG AGTGCAGCTG 
GAGAAGATGT TTGAGGCGAT GGGCGGGAAG GAGCTGGATG CCGAGGCCAG TGGCACCCTG 
AAGGAGCTGC AGGTGAAACT CAACAATGTC CTGGATGAAC TCAGCCATGT GTTTGCCACC 
AGCTTCCAGC CACACATTGA GGAATGCGTC AGACAGATGG GTGACATCCT GAGCCAGGTG 
AAGGGCACAG GCAATGTACC TGCCAGTGCC TGCAGCAGCG TGGCCCAGGA TGCAGACAAT 
GTGCTGCAGC CCATCATGGA TCTTCTGGAC AGCAACCTCA CCCTGTTTGC CAAAATCTGT 
GAGAAGACAG TTCTGAAGCG GGTGCTGAAG GAGCTCTGGA AGCTGGTGAT GAACACAATG 
GAGAAGACCA TCGTCCTGCC ACCGCTCACT GACCAGACGA TGATTGGCAC CCTCTTGAGA 
AAACATGGCA AGGGCCTAGA AAAGGGCAGG GTGAAACTGC CAAGCCACTC AGACGGGACA 
CAAATGATCT TCAATGCTGC CAAGGAGCTG GGCCAGCTGT CCAAACTTAA GGATCACATG 
GTGCGGGAAG AAGCCAAGAG CTTGACCCCG AAGCAGTGTG CTGTTGTTGA ACTGGCCCTG 
GACACCATCA AGCAATACTT CCACGCGGGG GGCGTTGGCC TCAAGAAGAC CTTCCTGGAG 
AAGAGCCCGG ACCTCCAGTC CCTGCGCTAC GCCCTGTCGC TCTACACGCA AGCCACCGAC 
CTGCTCATCA AAACCTTCGT GCAGACGCAG TCAGCGCAGG GCTCTGGTGT GGAGGACCCT 
GTAGGTGAAG TGTCCGTCCA CGTGGAGCTG TTCACACATC CAGGAACTGG GGAACAGAAG 
GTCACAGTGA AGGTGGTGGC CGCCAATGAC CTCAAGTGGC AGACTTCTGG CATCTTCCGC 
CCGTTCATCG AGGTCAACAT CGTTGGACCT CAGCTTAGCG ACAAGAAACG CAAGTTCGCC 
ACCAAATCCA AAAACAACAG CTGGGCGCCC AAGTATAACG AGAGCTTCCA GTTCTCCCTG 
AGCGCCGACG CGGGCCCCGA GTGCTACGAG CTGCAGGTGT GCGTGAAGGA CTACTGCTTT 
GCGCGCGAAG ACCGCACGGT GGGGCTGGCG GTGCTGCAAC TGCGAGAGCT GGCCCAGCGC 
GGGAGCGCCG CGTGCTGGCT TCCACTCGGC CGCCGCATCC ACATGGACGA CACGGGGCTC 
ACAGTGCTGC GCATCCTGTC GCAGCGCAGC AACGATGAGG TGGCCAAGGA ATTCGTCAAG 
CTCAAGTCGG ACACGCGCTC AGCCGAGGAG GGCGGTGCCG CGCCTGCGCC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168366.1
CDS	127..5280
Translation	MSLLCVGVKKAKFDGAQEKFNTYVTLKVQNVKSTTIAVRGSQPSWEQDFMFEINRLDLGLTVEVWNKGLIWDTMVGTVWIPLRTIRQSNEEGPGEWLTLDSQAIMADSEICGTKDPTFHRILLDAHFELPLDIPEEEARYWAKKLEQLNAMRDQDEVSHLQYSFQDQQDKPLPVPSSQCCNWNYFGWGEQNDDPDSAVDDRDSDYRSETSNSIPPPYYTTSQPNASVHQYSVRPPPLGSRESYSDSMHSYEEFSEPRALSPTGSSRYASSGELSQGSSQLSEDFDPDEHSLQGSELDDERDRDSYHSCHSSVSYHKDSPRWDQDDEDLEDLEDLEDEELPEEEEELEEEGEEELEEEDLEEEEEVPDDLASYTQQEDTTVAEPKEFKRISFPTAAPQKDDKVSAVPTEAPEVAKGIPKAATPEEKAAAERAQEAEPPKSEESFRSREEEEGQEGQDAMSRAKANWLRAFNKVRMQLQEARGEGDMSKSLWFKGGPGGGLIIIDSMPDIRKRKPIPLVSDLAMSLVQSRKAGITSALASSTLNNEELKNHVYKKTLQALIYPISCTTPHNFEVWTATTPTYCYECEGLLWGIARQGMRCTECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMVLKDRMKIRERNKPEIFELIQEIFAVTKSAHTQQMKAVKQSVLDGTSKWSAKISITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIYGNLNPVWEENFHFECHNSSDRIKVRVWDEDDDIKSRVKQRFKRESDDFLGQTIIEVRTLSGEMDVWYNLDKRTDKSAVSGAIRLHISVEIKGEEKVAPYHVQYTCLHENLFHFVTDVQNNGVVKIPDAKGDDAWKVYYDETAQEIVDEFAMRYGVESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSMYRNNFPASSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHELYGREYQTDPAKKGEVPPEEQGPSIKNLDFWSKLITLIVSIIEEDKNSYTPCLNQFPQELNVGKISAEVMWSLFAQDMKYAMEEHDKHRLCKSADYMNLHFKVKWLYNEYVAELPTFKDRVPEYPAWFEPFVIQWLDENEEVSRDFLHGALERDKKDGFQQTSEHALFSCSVVDVFSQLNQSFEIIKKLECPDPQIVGHYMRRFAKTISNVLLQYADIVSKDFASYCSKEKEKVPCILMNNTQQLRVQLEKMFEAMGGKELDAEASGTLKELQVKLNNVLDELSHVFATSFQPHIEECVRQMGDILSQVKGTGNVPASACSSVAQDADNVLQPIMDLLDSNLTLFAKICEKTVLKRVLKELWKLVMNTMEKTIVLPPLTDQTMIGTLLRKHGKGLEKGRVKLPSHSDGTQMIFNAAKELGQLSKLKDHMVREEAKSLTPKQCAVVELALDTIKQYFHAGGVGLKKTFLEKSPDLQSLRYALSLYTQATDLLIKTFVQTQSAQGSGVEDPVGEVSVHVELFTHPGTGEQKVTVKVVAANDLKWQTSGIFRPFIEVNIVGPQLSDKKRKFATKSKNNSWAPKYNESFQFSLSADAGPECYELQVCVKDYCFAREDRTVGLAVLQLRELAQRGSAACWLPLGRRIHMDDTGLTVLRILSQRSNDEVAKEFVKLKSDTRSAEEGGAAPAP

Target ORF information:

RefSeq Version	XM_017312877.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus unc-13 homolog A (Unc13a), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312877.2

ORF Insert Sequence:

ATGTCTCTGC TGTGCGTGGG AGTCAAAAAA GCCAAGTTTG ACGGTGCCCA AGAGAAGTTC 
AACACATACG TGACGCTGAA GGTGCAGAAC GTGAAGAGCA CTACCATAGC TGTACGTGGC 
AGCCAGCCCA GCTGGGAGCA GGACTTCATG TTTGAGATCA ACCGCCTGGA TCTAGGCCTG 
ACCGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGTAC TGTGTGGATC 
CCGCTGCGGA CCATCCGCCA GTCCAATGAG GAGGGCCCAG GAGAGTGGCT CACACTGGAC 
TCTCAGGCCA TCATGGCGGA CAGTGAGATC TGTGGGACCA AGGACCCCAC CTTCCATCGC 
ATCCTCCTGG ACGCTCATTT CGAGCTGCCG TTGGACATCC CTGAGGAGGA GGCACGCTAC 
TGGGCCAAGA AGCTGGAGCA GCTGAATGCC ATGCGTGACC AGGATGAGGT GAGTCACCTC 
CAGTACTCCT TCCAGGACCA GCAGGACAAG CCGCTGCCAG TGCCCAGCAG CCAGTGCTGC 
AACTGGAATT ACTTTGGCTG GGGAGAACAG AATGACGACC CCGACAGTGC GGTGGATGAC 
CGGGACAGTG ACTATAGGAG TGAGACCAGC AACAGCATCC CACCGCCCTA CTATACGACT 
TCGCAGCCCA ACGCCTCTGT GCACCAGTAC TCCGTGCGAC CGCCCCCTCT GGGGTCCCGG 
GAGTCCTACA GTGACTCCAT GCACAGCTAT GAGGAGTTCT CTGAGCCGCG GGCGCTCAGC 
CCCACAGGCA GCAGCCGCTA TGCTTCTTCC GGGGAGCTGA GCCAGGGAAG CTCACAGCTG 
AGCGAGGACT TCGACCCTGA TGAGCACAGC CTGCAGGGCT CGGAGCTGGA TGACGAGAGG 
GACCGCGATT CTTATCACTC CTGTCACAGC TCCGTGAGTT ACCACAAGGA CTCACCCCGC 
TGGGACCAGG ACGATGAGGA CCTGGAGGAC CTGGAGGACC TGGAAGATGA GGAGCTGCCC 
GAGGAGGAGG AGGAGTTGGA GGAGGAGGGG GAGGAGGAAT TGGAGGAAGA GGATTTGGAG 
GAGGAGGAGG AGGTGCCTGA TGACCTGGCC AGCTACACCC AGCAGGAGGA CACCACTGTG 
GCTGAGCCCA AAGAGTTCAA GCGGATCAGC TTCCCAACGG CTGCGCCTCA GAAGGATGAC 
AAGGTTTCAG CTGTGCCTAC AGAGGCCCCC GAGGTGGCCA AAGGCATCCC CAAAGCAGCC 
ACACCCGAGG AGAAGGCAGC TGCAGAGCGT GCGCAGGAAG CCGAGCCCCC CAAGTCTGAG 
GAGAGTTTCA GATCTCGAGA GGAGGAAGAG GGCCAGGAAG GGCAGGATGC CATGTCCAGA 
GCCAAAGCCA ACTGGTTGCG AGCCTTCAAC AAGGTGCGCA TGCAGCTGCA GGAGGCCCGA 
GGAGAAGGGG ATATGTCTAA GTCTCTATGG TTCAAAGGCG GTCCTGGTGG TGGCCTTATC 
ATCATTGACA GCATGCCAGA CATCAGGAAA CGGAAGCCCA TTCCCCTCGT GAGCGACCTG 
GCTATGTCAT TGGTGCAGTC TCGGAAGGCA GGCATCACCT CGGCCTTGGC CTCCAGCACG 
TTGAACAATG AAGAGCTGAA AAATCACGTT TACAAGAAGA CCCTGCAAGC CTTAATCTAC 
CCCATCTCCT GCACCACGCC GCACAATTTC GAGGTGTGGA CGGCCACCAC GCCCACCTAC 
TGCTATGAGT GCGAGGGGCT GCTGTGGGGC ATCGCGCGGC AGGGCATGCG ATGCACCGAG 
TGCGGCGTTA AGTGCCACGA GAAGTGCCAG GACCTGCTCA ATGCGGATTG CCTGCAGCGG 
GCGGCTGAGA AGAGTTCCAA GCATGGCGCT GAAGACCGCA CGCAGAACAT CATCATGGTG 
CTGAAGGACC GCATGAAGAT CCGCGAGCGC AACAAGCCTG AGATCTTCGA GCTTATCCAA 
GAGATCTTCG CGGTCACCAA GAGCGCACAC ACACAGCAGA TGAAAGCTGT CAAGCAGAGT 
GTGCTGGACG GCACATCCAA GTGGTCTGCC AAAATTAGCA TCACAGTGGT CTGTGCCCAG 
GGCTTGCAGG CCAAGGACAA GACAGGATCC AGTGACCCTT ATGTCACCGT CCAGGTTGGG 
AAGACCAAGA AAAGGACAAA AACCATCTAC GGGAACCTCA ACCCAGTGTG GGAAGAGAAT 
TTTCATTTTG AATGTCACAA CTCCTCTGAC CGGATCAAGG TTCGTGTTTG GGATGAAGAT 
GACGACATAA AATCCCGTGT GAAACAAAGG TTTAAGAGGG AGTCTGATGA CTTCCTAGGG 
CAGACAATCA TCGAGGTGCG GACGCTTAGC GGCGAGATGG ATGTGTGGTA CAATCTGGAC 
AAGAGAACGG ACAAGTCGGC GGTGTCGGGG GCCATCCGGC TTCACATCAG TGTGGAGATC 
AAAGGGGAGG AGAAGGTGGC GCCCTACCAT GTCCAGTACA CCTGTCTGCA TGAGAACCTG 
TTCCACTTTG TGACGGACGT GCAGAACAAT GGTGTGGTGA AGATTCCCGA TGCCAAGGGT 
GACGACGCCT GGAAGGTTTA CTACGATGAG ACGGCCCAGG AGATCGTGGA TGAGTTTGCC 
ATGCGCTATG GTGTTGAGTC CATCTACCAA GCCATGACCC ACTTTGCCTG CCTCTCCTCC 
AAGTATATGT GCCCTGGGGT ACCCGCTGTC ATGAGCACCC TGCTTGCCAA CATCAACGCC 
TACTACGCAC ACACCACCGC CTCCACCAAC GTGTCTGCCT CTGACCGCTT CGCTGCCTCT 
AATTTCGGGA AAGAGCGCTT TGTGAAACTT CTGGACCAGC TACACAATTC TCTGCGGATC 
GACCTGTCCA TGTACCGGAA CAACTTCCCA GCCAGCAGCC CTGAGCGGCT GCAGGATCTC 
AAGTCCACGG TGGACCTGCT CACCAGCATC ACCTTCTTCC GGATGAAGGT TCAGGAACTG 
CAGAGCCCGC CGCGTGCCAG CCAGGTAGTG AAAGACTGTG TGAAGGCCTG CCTCAACTCC 
ACCTATGAGT ACATCTTCAA CAACTGCCAT GAGCTCTATG GCCGGGAGTA CCAAACGGAT 
CCGGCCAAGA AGGGGGAGGT TCCCCCAGAG GAGCAAGGCC CTAGCATCAA GAACCTGGAT 
TTCTGGTCCA AGCTGATCAC CCTCATCGTG TCTATCATCG AGGAGGATAA GAATTCTTAC 
ACACCCTGCC TCAATCAGTT TCCCCAGGAG CTCAATGTGG GGAAGATCAG TGCTGAGGTG 
ATGTGGAGCC TATTTGCCCA AGACATGAAG TACGCCATGG AGGAACATGA CAAACACCGG 
CTGTGTAAGA GCGCAGATTA CATGAACCTG CACTTCAAGG TGAAGTGGCT GTACAATGAG 
TACGTGGCCG AGCTGCCCAC CTTCAAGGAC CGTGTGCCTG AGTACCCTGC GTGGTTTGAG 
CCCTTCGTCA TCCAGTGGTT GGATGAGAAT GAGGAGGTGT CCCGGGACTT CCTGCATGGT 
GCCCTCGAGC GGGACAAGAA GGACGGGTTC CAGCAGACCT CAGAGCACGC CCTGTTCTCT 
TGCTCAGTGG TGGACGTCTT CTCCCAGCTT AACCAGAGTT TCGAGATCAT CAAGAAGCTG 
GAGTGTCCTG ACCCCCAGAT CGTGGGCCAT TACATGCGGC GTTTTGCCAA GACCATTAGC 
AATGTGCTTC TCCAGTATGC AGACATCGTT TCCAAGGACT TCGCCTCCTA CTGCTCCAAG 
GAGAAGGAGA AAGTGCCCTG CATCCTCATG AACAACACAC AGCAACTACG AGTGCAGCTG 
GAGAAGATGT TTGAGGCGAT GGGCGGGAAG GAGCTGGATG CCGAGGCCAG TGGCACCCTG 
AAGGAGCTGC AGGTGAAACT CAACAATGTC CTGGATGAAC TCAGCCATGT GTTTGCCACC 
AGCTTCCAGC CACACATTGA GGAATGCGTC AGACAGATGG GTGACATCCT GAGCCAGGTG 
AAGGGCACAG GCAATGTACC TGCCAGTGCC TGCAGCAGCG TGGCCCAGGA TGCAGACAAT 
GTGCTGCAGC CCATCATGGA TCTTCTGGAC AGCAACCTCA CCCTGTTTGC CAAAATCTGT 
GAGAAGACAG TTCTGAAGCG GGTGCTGAAG GAGCTCTGGA AGCTGGTGAT GAACACAATG 
GAGAAGACCA TCGTCCTGCC ACCGCTCACT GACCAGACGA TGATTGGCAC CCTCTTGAGA 
AAACATGGCA AGGGCCTAGA AAAGGGCAGG GTGAAACTGC CAAGCCACTC AGACGGGACA 
CAAATGATCT TCAATGCTGC CAAGGAGCTG GGCCAGCTGT CCAAACTTAA GGATCACATG 
GTGCGGGAAG AAGCCAAGAG CTTGACCCCG AAGCAGTGTG CTGTTGTTGA ACTGGCCCTG 
GACACCATCA AGCAATACTT CCACGCGGGG GGCGTTGGCC TCAAGAAGAC CTTCCTGGAG 
AAGAGCCCGG ACCTCCAGTC CCTGCGCTAC GCCCTGTCGC TCTACACGCA AGCCACCGAC 
CTGCTCATCA AAACCTTCGT GCAGACGCAG TCAGCGCAGG GCTCTGGTGT GGAGGACCCT 
GTAGGTGAAG TGTCCGTCCA CGTGGAGCTG TTCACACATC CAGGAACTGG GGAACAGAAG 
GTCACAGTGA AGGTGGTGGC CGCCAATGAC CTCAAGTGGC AGACTTCTGG CATCTTCCGC 
CCGTTCATCG AGGTCAACAT CGTTGGACCT CAGCTTAGCG ACAAGAAACG CAAGTTCGCC 
ACCAAATCCA AAAACAACAG CTGGGCGCCC AAGTATAACG AGAGCTTCCA GTTCTCCCTG 
AGCGCCGACG CGGGCCCCGA GTGCTACGAG CTGCAGGTGT GCGTGAAGGA CTACTGCTTT 
GCGCGCGAAG ACCGCACGGT GGGGCTGGCG GTGCTGCAAC TGCGAGAGCT GGCCCAGCGC 
GGGAGCGCCG CGTGCTGGCT TCCACTCGGC CGCCGCATCC ACATGGACGA CACGGGGCTC 
ACAGTGCTGC GCATCCTGTC GCAGCGCAGC AACGATGAGG TGGCCAAGGA ATTCGTCAAG 
CTCAAGTCGG ACACGCGCTC AGCCGAGGAG GGCGGTGCCG CGCCTGCGCC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Unc13a ( XM_017312878.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168367.1 Mus musculus unc-13 homolog A (Unc13a), transcript variant X7, mRNA.

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CloneID	OMu82528
Clone ID Related Accession (Same CDS sequence)	XM_017312878.1 , XM_017312878.2
Accession Version	XM_017312878.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5142bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein unc-13 homolog A isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017312878.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTGC TGTGCGTGGG AGTCAAAAAA GCCAAGTTTG ACGGTGCCCA AGAGAAGTTC 
AACACATACG TGACGCTGAA GGTGCAGAAC GTGAAGAGCA CTACCATAGC TGTACGTGGC 
AGCCAGCCCA GCTGGGAGCA GGACTTCATG TTTGAGATCA ACCGCCTGGA TCTAGGCCTG 
ACCGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGTAC TGTGTGGATC 
CCGCTGCGGA CCATCCGCCA GTCCAATGAG GAGGGCCCAG GAGAGTGGCT CACACTGGAC 
TCTCAGGCCA TCATGGCGGA CAGTGAGATC TGTGGGACCA AGGACCCCAC CTTCCATCGC 
ATCCTCCTGG ACGCTCATTT CGAGCTGCCG TTGGACATCC CTGAGGAGGA GGCACGCTAC 
TGGGCCAAGA AGCTGGAGCA GCTGAATGCC ATGCGTGACC AGGATGAGGT GAGTCACCTC 
CAGTACTCCT TCCAGGACCA GCAGGACAAG CCGCTGCCAG TGCCCAGCAG CCAGTGCTGC 
AACTGGAATT ACTTTGGCTG GGGAGAACAG AATGACGACC CCGACAGTGC GGTGGATGAC 
CGGGACAGTG ACTATAGGAG TGAGACCAGC AACAGCATCC CACCGCCCTA CTATACGACT 
TCGCAGCCCA ACGCCTCTGT GCACCAGTAC TCCGTGCGAC CGCCCCCTCT GGGGTCCCGG 
GAGTCCTACA GTGACTCCAT GCACAGCTAT GAGGAGTTCT CTGAGCCGCG GGCGCTCAGC 
CCCACAGGCA GCAGCCGCTA TGCTTCTTCC GGGGAGCTGA GCCAGGGAAG CTCACAGCTG 
AGCGAGGACT TCGACCCTGA TGAGCACAGC CTGCAGGGCT CGGAGCTGGA TGACGAGAGG 
GACCGCGATT CTTATCACTC CTGTCACAGC TCCGTGAGTT ACCACAAGGA CTCACCCCGC 
TGGGACCAGG ACGATGAGGA CCTGGAGGAC CTGGAGGACC TGGAAGATGA GGAGCTGCCC 
GAGGAGGAGG AGGAGTTGGA GGAGGAGGGG GAGGAGGAAT TGGAGGAAGA GGATTTGGAG 
GAGGAGGAGG AGGTGCCTGA TGACCTGGCC AGCTACACCC AGCAGGAGGA CACCACTGTG 
GCTGAGCCCA AAGAGTTCAA GCGGATCAGC TTCCCAACGG CTGCGCCTCA GAAGGATGAC 
AAGGTTTCAG CTGTGCCTAC AGAGGCCCCC GAGGTGGCCA AAGGCATCCC CAAAGCAGCC 
ACACCCGAGG AGAAGGCAGC TGCAGAGCGT GCGCAGGAAG CCGAGCCCCC CAAGTCTGAG 
GAGAGTTTCA GATCTCGAGA GGAGGAAGAG GGCCAGGAAG GGCAGGATGC CATGTCCAGA 
GCCAAAGCCA ACTGGTTGCG AGCCTTCAAC AAGGTGCGCA TGCAGCTGCA GGAGGCCCGA 
GGAGAAGGGG ATATGTCTAA GTCTCTATGG TTCAAAGGCG GTCCTGGTGG TGGCCTTATC 
ATCATTGACA GCATGCCAGA CATCAGGAAA CGGAAGCCCA TTCCCCTCGT GAGCGACCTG 
GCTATGTCAT TGGTGCAGTC TCGGAAGGCA GGCATCACCT CGGCCTTGGC CTCCAGCACG 
TTGAACAATG AAGAGCTGAA AAATCACGTT TACAAGAAGA CCCTGCAAGC CTTAATCTAC 
CCCATCTCCT GCACCACGCC GCACAATTTC GAGGTGTGGA CGGCCACCAC GCCCACCTAC 
TGCTATGAGT GCGAGGGGCT GCTGTGGGGC ATCGCGCGGC AGGGCATGCG ATGCACCGAG 
TGCGGCGTTA AGTGCCACGA GAAGTGCCAG GACCTGCTCA ATGCGGATTG CCTGCAGCGG 
GCGGCTGAGA AGAGTTCCAA GCATGGCGCT GAAGACCGCA CGCAGAACAT CATCATGGTG 
CTGAAGGACC GCATGAAGAT CCGCGAGCGC AACAAGCCTG AGATCTTCGA GCTTATCCAA 
GAGATCTTCG CGGTCACCAA GAGCGCACAC ACACAGCAGA TGAAAGCTGT CAAGCAGAGT 
GTGCTGGACG GCACATCCAA GTGGTCTGCC AAAATTAGCA TCACAGTGGT CTGTGCCCAG 
GGCTTGCAGG CCAAGGACAA GACAGGATCC AGTGACCCTT ATGTCACCGT CCAGGTTGGG 
AAGACCAAGA AAAGGACAAA AACCATCTAC GGGAACCTCA ACCCAGTGTG GGAAGAGAAT 
TTTCATTTTG AATGTCACAA CTCCTCTGAC CGGATCAAGG TTCGTGTTTG GGATGAAGAT 
GACGACATAA AATCCCGTGT GAAACAAAGG TTTAAGAGGG AGTCTGATGA CTTCCTAGGG 
CAGACAATCA TCGAGGTGCG GACGCTTAGC GGCGAGATGG ATGTGTGGTA CAATCTGGAC 
AAGAGAACGG ACAAGTCGGC GGTGTCGGGG GCCATCCGGC TTCACATCAG TGTGGAGATC 
AAAGGGGAGG AGAAGGTGGC GCCCTACCAT GTCCAGTACA CCTGTCTGCA TGAGAACCTG 
TTCCACTTTG TGACGGACGT GCAGAACAAT GGTGTGGTGA AGATTCCCGA TGCCAAGGGT 
GACGACGCCT GGAAGGTTTA CTACGATGAG ACGGCCCAGG AGATCGTGGA TGAGTTTGCC 
ATGCGCTATG GTGTTGAGTC CATCTACCAA GCCATGACCC ACTTTGCCTG CCTCTCCTCC 
AAGTATATGT GCCCTGGGGT ACCCGCTGTC ATGAGCACCC TGCTTGCCAA CATCAACGCC 
TACTACGCAC ACACCACCGC CTCCACCAAC GTGTCTGCCT CTGACCGCTT CGCTGCCTCT 
AATTTCGGGA AAGAGCGCTT TGTGAAACTT CTGGACCAGC TACACAATTC TCTGCGGATC 
GACCTGTCCA TGTACCGGAA CAACTTCCCA GCCAGCAGCC CTGAGCGGCT GCAGGATCTC 
AAGTCCACGG TGGACCTGCT CACCAGCATC ACCTTCTTCC GGATGAAGGT TCAGGAACTG 
CAGAGCCCGC CGCGTGCCAG CCAGGTAGTG AAAGACTGTG TGAAGGCCTG CCTCAACTCC 
ACCTATGAGT ACATCTTCAA CAACTGCCAT GAGCTCTATG GCCGGGAGTA CCAAACGGAT 
CCGGCCAAGA AGGGGGAGGT TCCCCCAGAG GAGCAAGGCC CTAGCATCAA GAACCTGGAT 
TTCTGGTCCA AGCTGATCAC CCTCATCGTG TCTATCATCG AGGAGGATAA GAATTCTTAC 
ACACCCTGCC TCAATCAGTT TCCCCAGGAG CTCAATGTGG GGAAGATCAG TGCTGAGGTG 
ATGTGGAGCC TATTTGCCCA AGACATGAAG TACGCCATGG AGGAACATGA CAAACACCGG 
CTGTGTAAGA GCGCAGATTA CATGAACCTG CACTTCAAGG TGAAGTGGCT GTACAATGAG 
TACGTGGCCG AGCTGCCCAC CTTCAAGGAC CGTGTGCCTG AGTACCCTGC GTGGTTTGAG 
CCCTTCGTCA TCCAGTGGTT GGATGAGAAT GAGGAGGTGT CCCGGGACTT CCTGCATGGT 
GCCCTCGAGC GGGACAAGAA GGACGGGTTC CAGCAGACCT CAGAGCACGC CCTGTTCTCT 
TGCTCAGTGG TGGACGTCTT CTCCCAGCTT AACCAGAGTT TCGAGATCAT CAAGAAGCTG 
GAGTGTCCTG ACCCCCAGAT CGTGGGCCAT TACATGCGGC GTTTTGCCAA GACCATTAGC 
AATGTGCTTC TCCAGTATGC AGACATCGTT TCCAAGGACT TCGCCTCCTA CTGCTCCAAG 
GAGAAGGAGA AAGTGCCCTG CATCCTCATG AACAACACAC AGCAACTACG AGTGCAGCTG 
GAGAAGATGT TTGAGGCGAT GGGCGGGAAG GAGCTGGATG CCGAGGCCAG TGGCACCCTG 
AAGGAGCTGC AGGTGAAACT CAACAATGTC CTGGATGAAC TCAGCCATGT GTTTGCCACC 
AGCTTCCAGC CACACATTGA GGAATGCGTC AGACAGATGG GTGACATCCT GAGCCAGGTG 
AAGGGCACAG GCAATGTACC TGCCAGTGCC TGCAGCAGCG TGGCCCAGGA TGCAGACAAT 
GTGCTGCAGC CCATCATGGA TCTTCTGGAC AGCAACCTCA CCCTGTTTGC CAAAATCTGT 
GAGAAGACAG TTCTGAAGCG GGTGCTGAAG GAGCTCTGGA AGCTGGTGAT GAACACAATG 
GAGAAGACCA TCGTCCTGCC ACCGCTCACT GACCAGACGA TGATTGGGAC ACAAATGATC 
TTCAATGCTG CCAAGGAGCT GGGCCAGCTG TCCAAACTTA AGGATCACAT GGTGCGGGAA 
GAAGCCAAGA GCTTGACCCC GAAGCAGTGT GCTGTTGTTG AACTGGCCCT GGACACCATC 
AAGCAATACT TCCACGCGGG GGGCGTTGGC CTCAAGAAGA CCTTCCTGGA GAAGAGCCCG 
GACCTCCAGT CCCTGCGCTA CGCCCTGTCG CTCTACACGC AAGCCACCGA CCTGCTCATC 
AAAACCTTCG TGCAGACGCA GTCAGCGCAG GTCCATGGTG GAAAAGGGAC TAGGTTTACC 
CTTAGTGAAG ACGTTTGTCC TGAGATGGGC TCTGGTGTGG AGGACCCTGT AGGTGAAGTG 
TCCGTCCACG TGGAGCTGTT CACACATCCA GGAACTGGGG AACAGAAGGT CACAGTGAAG 
GTGGTGGCCG CCAATGACCT CAAGTGGCAG ACTTCTGGCA TCTTCCGCCC GTTCATCGAG 
GTCAACATCG TTGGACCTCA GCTTAGCGAC AAGAAACGCA AGTTCGCCAC CAAATCCAAA 
AACAACAGCT GGGCGCCCAA GTATAACGAG AGCTTCCAGT TCTCCCTGAG CGCCGACGCG 
GGCCCCGAGT GCTACGAGCT GCAGGTGTGC GTGAAGGACT ACTGCTTTGC GCGCGAAGAC 
CGCACGGTGG GGCTGGCGGT GCTGCAACTG CGAGAGCTGG CCCAGCGCGG GAGCGCCGCG 
TGCTGGCTTC CACTCGGCCG CCGCATCCAC ATGGACGACA CGGGGCTCAC AGTGCTGCGC 
ATCCTGTCGC AGCGCAGCAA CGATGAGGTG GCCAAGGAAT TCGTCAAGCT CAAGTCGGAC 
ACGCGCTCAG CCGAGGAGGG CGGTGCCGCG CCTGCGCCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168367.1
CDS	127..5268
Translation	MSLLCVGVKKAKFDGAQEKFNTYVTLKVQNVKSTTIAVRGSQPSWEQDFMFEINRLDLGLTVEVWNKGLIWDTMVGTVWIPLRTIRQSNEEGPGEWLTLDSQAIMADSEICGTKDPTFHRILLDAHFELPLDIPEEEARYWAKKLEQLNAMRDQDEVSHLQYSFQDQQDKPLPVPSSQCCNWNYFGWGEQNDDPDSAVDDRDSDYRSETSNSIPPPYYTTSQPNASVHQYSVRPPPLGSRESYSDSMHSYEEFSEPRALSPTGSSRYASSGELSQGSSQLSEDFDPDEHSLQGSELDDERDRDSYHSCHSSVSYHKDSPRWDQDDEDLEDLEDLEDEELPEEEEELEEEGEEELEEEDLEEEEEVPDDLASYTQQEDTTVAEPKEFKRISFPTAAPQKDDKVSAVPTEAPEVAKGIPKAATPEEKAAAERAQEAEPPKSEESFRSREEEEGQEGQDAMSRAKANWLRAFNKVRMQLQEARGEGDMSKSLWFKGGPGGGLIIIDSMPDIRKRKPIPLVSDLAMSLVQSRKAGITSALASSTLNNEELKNHVYKKTLQALIYPISCTTPHNFEVWTATTPTYCYECEGLLWGIARQGMRCTECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMVLKDRMKIRERNKPEIFELIQEIFAVTKSAHTQQMKAVKQSVLDGTSKWSAKISITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIYGNLNPVWEENFHFECHNSSDRIKVRVWDEDDDIKSRVKQRFKRESDDFLGQTIIEVRTLSGEMDVWYNLDKRTDKSAVSGAIRLHISVEIKGEEKVAPYHVQYTCLHENLFHFVTDVQNNGVVKIPDAKGDDAWKVYYDETAQEIVDEFAMRYGVESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSMYRNNFPASSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHELYGREYQTDPAKKGEVPPEEQGPSIKNLDFWSKLITLIVSIIEEDKNSYTPCLNQFPQELNVGKISAEVMWSLFAQDMKYAMEEHDKHRLCKSADYMNLHFKVKWLYNEYVAELPTFKDRVPEYPAWFEPFVIQWLDENEEVSRDFLHGALERDKKDGFQQTSEHALFSCSVVDVFSQLNQSFEIIKKLECPDPQIVGHYMRRFAKTISNVLLQYADIVSKDFASYCSKEKEKVPCILMNNTQQLRVQLEKMFEAMGGKELDAEASGTLKELQVKLNNVLDELSHVFATSFQPHIEECVRQMGDILSQVKGTGNVPASACSSVAQDADNVLQPIMDLLDSNLTLFAKICEKTVLKRVLKELWKLVMNTMEKTIVLPPLTDQTMIGTQMIFNAAKELGQLSKLKDHMVREEAKSLTPKQCAVVELALDTIKQYFHAGGVGLKKTFLEKSPDLQSLRYALSLYTQATDLLIKTFVQTQSAQVHGGKGTRFTLSEDVCPEMGSGVEDPVGEVSVHVELFTHPGTGEQKVTVKVVAANDLKWQTSGIFRPFIEVNIVGPQLSDKKRKFATKSKNNSWAPKYNESFQFSLSADAGPECYELQVCVKDYCFAREDRTVGLAVLQLRELAQRGSAACWLPLGRRIHMDDTGLTVLRILSQRSNDEVAKEFVKLKSDTRSAEEGGAAPAP

Target ORF information:

RefSeq Version	XM_017312878.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus unc-13 homolog A (Unc13a), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312878.2

ORF Insert Sequence:

ATGTCTCTGC TGTGCGTGGG AGTCAAAAAA GCCAAGTTTG ACGGTGCCCA AGAGAAGTTC 
AACACATACG TGACGCTGAA GGTGCAGAAC GTGAAGAGCA CTACCATAGC TGTACGTGGC 
AGCCAGCCCA GCTGGGAGCA GGACTTCATG TTTGAGATCA ACCGCCTGGA TCTAGGCCTG 
ACCGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGTAC TGTGTGGATC 
CCGCTGCGGA CCATCCGCCA GTCCAATGAG GAGGGCCCAG GAGAGTGGCT CACACTGGAC 
TCTCAGGCCA TCATGGCGGA CAGTGAGATC TGTGGGACCA AGGACCCCAC CTTCCATCGC 
ATCCTCCTGG ACGCTCATTT CGAGCTGCCG TTGGACATCC CTGAGGAGGA GGCACGCTAC 
TGGGCCAAGA AGCTGGAGCA GCTGAATGCC ATGCGTGACC AGGATGAGGT GAGTCACCTC 
CAGTACTCCT TCCAGGACCA GCAGGACAAG CCGCTGCCAG TGCCCAGCAG CCAGTGCTGC 
AACTGGAATT ACTTTGGCTG GGGAGAACAG AATGACGACC CCGACAGTGC GGTGGATGAC 
CGGGACAGTG ACTATAGGAG TGAGACCAGC AACAGCATCC CACCGCCCTA CTATACGACT 
TCGCAGCCCA ACGCCTCTGT GCACCAGTAC TCCGTGCGAC CGCCCCCTCT GGGGTCCCGG 
GAGTCCTACA GTGACTCCAT GCACAGCTAT GAGGAGTTCT CTGAGCCGCG GGCGCTCAGC 
CCCACAGGCA GCAGCCGCTA TGCTTCTTCC GGGGAGCTGA GCCAGGGAAG CTCACAGCTG 
AGCGAGGACT TCGACCCTGA TGAGCACAGC CTGCAGGGCT CGGAGCTGGA TGACGAGAGG 
GACCGCGATT CTTATCACTC CTGTCACAGC TCCGTGAGTT ACCACAAGGA CTCACCCCGC 
TGGGACCAGG ACGATGAGGA CCTGGAGGAC CTGGAGGACC TGGAAGATGA GGAGCTGCCC 
GAGGAGGAGG AGGAGTTGGA GGAGGAGGGG GAGGAGGAAT TGGAGGAAGA GGATTTGGAG 
GAGGAGGAGG AGGTGCCTGA TGACCTGGCC AGCTACACCC AGCAGGAGGA CACCACTGTG 
GCTGAGCCCA AAGAGTTCAA GCGGATCAGC TTCCCAACGG CTGCGCCTCA GAAGGATGAC 
AAGGTTTCAG CTGTGCCTAC AGAGGCCCCC GAGGTGGCCA AAGGCATCCC CAAAGCAGCC 
ACACCCGAGG AGAAGGCAGC TGCAGAGCGT GCGCAGGAAG CCGAGCCCCC CAAGTCTGAG 
GAGAGTTTCA GATCTCGAGA GGAGGAAGAG GGCCAGGAAG GGCAGGATGC CATGTCCAGA 
GCCAAAGCCA ACTGGTTGCG AGCCTTCAAC AAGGTGCGCA TGCAGCTGCA GGAGGCCCGA 
GGAGAAGGGG ATATGTCTAA GTCTCTATGG TTCAAAGGCG GTCCTGGTGG TGGCCTTATC 
ATCATTGACA GCATGCCAGA CATCAGGAAA CGGAAGCCCA TTCCCCTCGT GAGCGACCTG 
GCTATGTCAT TGGTGCAGTC TCGGAAGGCA GGCATCACCT CGGCCTTGGC CTCCAGCACG 
TTGAACAATG AAGAGCTGAA AAATCACGTT TACAAGAAGA CCCTGCAAGC CTTAATCTAC 
CCCATCTCCT GCACCACGCC GCACAATTTC GAGGTGTGGA CGGCCACCAC GCCCACCTAC 
TGCTATGAGT GCGAGGGGCT GCTGTGGGGC ATCGCGCGGC AGGGCATGCG ATGCACCGAG 
TGCGGCGTTA AGTGCCACGA GAAGTGCCAG GACCTGCTCA ATGCGGATTG CCTGCAGCGG 
GCGGCTGAGA AGAGTTCCAA GCATGGCGCT GAAGACCGCA CGCAGAACAT CATCATGGTG 
CTGAAGGACC GCATGAAGAT CCGCGAGCGC AACAAGCCTG AGATCTTCGA GCTTATCCAA 
GAGATCTTCG CGGTCACCAA GAGCGCACAC ACACAGCAGA TGAAAGCTGT CAAGCAGAGT 
GTGCTGGACG GCACATCCAA GTGGTCTGCC AAAATTAGCA TCACAGTGGT CTGTGCCCAG 
GGCTTGCAGG CCAAGGACAA GACAGGATCC AGTGACCCTT ATGTCACCGT CCAGGTTGGG 
AAGACCAAGA AAAGGACAAA AACCATCTAC GGGAACCTCA ACCCAGTGTG GGAAGAGAAT 
TTTCATTTTG AATGTCACAA CTCCTCTGAC CGGATCAAGG TTCGTGTTTG GGATGAAGAT 
GACGACATAA AATCCCGTGT GAAACAAAGG TTTAAGAGGG AGTCTGATGA CTTCCTAGGG 
CAGACAATCA TCGAGGTGCG GACGCTTAGC GGCGAGATGG ATGTGTGGTA CAATCTGGAC 
AAGAGAACGG ACAAGTCGGC GGTGTCGGGG GCCATCCGGC TTCACATCAG TGTGGAGATC 
AAAGGGGAGG AGAAGGTGGC GCCCTACCAT GTCCAGTACA CCTGTCTGCA TGAGAACCTG 
TTCCACTTTG TGACGGACGT GCAGAACAAT GGTGTGGTGA AGATTCCCGA TGCCAAGGGT 
GACGACGCCT GGAAGGTTTA CTACGATGAG ACGGCCCAGG AGATCGTGGA TGAGTTTGCC 
ATGCGCTATG GTGTTGAGTC CATCTACCAA GCCATGACCC ACTTTGCCTG CCTCTCCTCC 
AAGTATATGT GCCCTGGGGT ACCCGCTGTC ATGAGCACCC TGCTTGCCAA CATCAACGCC 
TACTACGCAC ACACCACCGC CTCCACCAAC GTGTCTGCCT CTGACCGCTT CGCTGCCTCT 
AATTTCGGGA AAGAGCGCTT TGTGAAACTT CTGGACCAGC TACACAATTC TCTGCGGATC 
GACCTGTCCA TGTACCGGAA CAACTTCCCA GCCAGCAGCC CTGAGCGGCT GCAGGATCTC 
AAGTCCACGG TGGACCTGCT CACCAGCATC ACCTTCTTCC GGATGAAGGT TCAGGAACTG 
CAGAGCCCGC CGCGTGCCAG CCAGGTAGTG AAAGACTGTG TGAAGGCCTG CCTCAACTCC 
ACCTATGAGT ACATCTTCAA CAACTGCCAT GAGCTCTATG GCCGGGAGTA CCAAACGGAT 
CCGGCCAAGA AGGGGGAGGT TCCCCCAGAG GAGCAAGGCC CTAGCATCAA GAACCTGGAT 
TTCTGGTCCA AGCTGATCAC CCTCATCGTG TCTATCATCG AGGAGGATAA GAATTCTTAC 
ACACCCTGCC TCAATCAGTT TCCCCAGGAG CTCAATGTGG GGAAGATCAG TGCTGAGGTG 
ATGTGGAGCC TATTTGCCCA AGACATGAAG TACGCCATGG AGGAACATGA CAAACACCGG 
CTGTGTAAGA GCGCAGATTA CATGAACCTG CACTTCAAGG TGAAGTGGCT GTACAATGAG 
TACGTGGCCG AGCTGCCCAC CTTCAAGGAC CGTGTGCCTG AGTACCCTGC GTGGTTTGAG 
CCCTTCGTCA TCCAGTGGTT GGATGAGAAT GAGGAGGTGT CCCGGGACTT CCTGCATGGT 
GCCCTCGAGC GGGACAAGAA GGACGGGTTC CAGCAGACCT CAGAGCACGC CCTGTTCTCT 
TGCTCAGTGG TGGACGTCTT CTCCCAGCTT AACCAGAGTT TCGAGATCAT CAAGAAGCTG 
GAGTGTCCTG ACCCCCAGAT CGTGGGCCAT TACATGCGGC GTTTTGCCAA GACCATTAGC 
AATGTGCTTC TCCAGTATGC AGACATCGTT TCCAAGGACT TCGCCTCCTA CTGCTCCAAG 
GAGAAGGAGA AAGTGCCCTG CATCCTCATG AACAACACAC AGCAACTACG AGTGCAGCTG 
GAGAAGATGT TTGAGGCGAT GGGCGGGAAG GAGCTGGATG CCGAGGCCAG TGGCACCCTG 
AAGGAGCTGC AGGTGAAACT CAACAATGTC CTGGATGAAC TCAGCCATGT GTTTGCCACC 
AGCTTCCAGC CACACATTGA GGAATGCGTC AGACAGATGG GTGACATCCT GAGCCAGGTG 
AAGGGCACAG GCAATGTACC TGCCAGTGCC TGCAGCAGCG TGGCCCAGGA TGCAGACAAT 
GTGCTGCAGC CCATCATGGA TCTTCTGGAC AGCAACCTCA CCCTGTTTGC CAAAATCTGT 
GAGAAGACAG TTCTGAAGCG GGTGCTGAAG GAGCTCTGGA AGCTGGTGAT GAACACAATG 
GAGAAGACCA TCGTCCTGCC ACCGCTCACT GACCAGACGA TGATTGGGAC ACAAATGATC 
TTCAATGCTG CCAAGGAGCT GGGCCAGCTG TCCAAACTTA AGGATCACAT GGTGCGGGAA 
GAAGCCAAGA GCTTGACCCC GAAGCAGTGT GCTGTTGTTG AACTGGCCCT GGACACCATC 
AAGCAATACT TCCACGCGGG GGGCGTTGGC CTCAAGAAGA CCTTCCTGGA GAAGAGCCCG 
GACCTCCAGT CCCTGCGCTA CGCCCTGTCG CTCTACACGC AAGCCACCGA CCTGCTCATC 
AAAACCTTCG TGCAGACGCA GTCAGCGCAG GTCCATGGTG GAAAAGGGAC TAGGTTTACC 
CTTAGTGAAG ACGTTTGTCC TGAGATGGGC TCTGGTGTGG AGGACCCTGT AGGTGAAGTG 
TCCGTCCACG TGGAGCTGTT CACACATCCA GGAACTGGGG AACAGAAGGT CACAGTGAAG 
GTGGTGGCCG CCAATGACCT CAAGTGGCAG ACTTCTGGCA TCTTCCGCCC GTTCATCGAG 
GTCAACATCG TTGGACCTCA GCTTAGCGAC AAGAAACGCA AGTTCGCCAC CAAATCCAAA 
AACAACAGCT GGGCGCCCAA GTATAACGAG AGCTTCCAGT TCTCCCTGAG CGCCGACGCG 
GGCCCCGAGT GCTACGAGCT GCAGGTGTGC GTGAAGGACT ACTGCTTTGC GCGCGAAGAC 
CGCACGGTGG GGCTGGCGGT GCTGCAACTG CGAGAGCTGG CCCAGCGCGG GAGCGCCGCG 
TGCTGGCTTC CACTCGGCCG CCGCATCCAC ATGGACGACA CGGGGCTCAC AGTGCTGCGC 
ATCCTGTCGC AGCGCAGCAA CGATGAGGTG GCCAAGGAAT TCGTCAAGCT CAAGTCGGAC 
ACGCGCTCAG CCGAGGAGGG CGGTGCCGCG CCTGCGCCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Unc13a ( XM_017312879.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168368.1 Mus musculus unc-13 homolog A (Unc13a), transcript variant X8, mRNA.

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CloneID	OMu82529
Clone ID Related Accession (Same CDS sequence)	XM_017312879.1 , XM_017312879.2
Accession Version	XM_017312879.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5136bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein unc-13 homolog A isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017312879.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTGC TGTGCGTGGG AGTCAAAAAA GCCAAGTTTG ACGGTGCCCA AGAGAAGTTC 
AACACATACG TGACGCTGAA GGTGCAGAAC GTGAAGAGCA CTACCATAGC TGTACGTGGC 
AGCCAGCCCA GCTGGGAGCA GGACTTCATG TTTGAGATCA ACCGCCTGGA TCTAGGCCTG 
ACCGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGTAC TGTGTGGATC 
CCGCTGCGGA CCATCCGCCA GTCCAATGAG GAGGGCCCAG GAGAGTGGCT CACACTGGAC 
TCTCAGGCCA TCATGGCGGA CAGTGAGATC TGTGGGACCA AGGACCCCAC CTTCCATCGC 
ATCCTCCTGG ACGCTCATTT CGAGCTGCCG TTGGACATCC CTGAGGAGGA GGCACGCTAC 
TGGGCCAAGA AGCTGGAGCA GCTGAATGCC ATGCGTGACC AGGATGAGGT GAGTCACCTC 
CAGTACTCCT TCCAGGACCA GCAGGACAAG CCGCTGCCAG TGCCCAGCAG CCAGTGCTGC 
AACTGGAATT ACTTTGGCTG GGGAGAACAG AATGACGACC CCGACAGTGC GGTGGATGAC 
CGGGACAGTG ACTATAGGAG TGAGACCAGC AACAGCATCC CACCGCCCTA CTATACGACT 
TCGCAGCCCA ACGCCTCTGT GCACCAGTAC TCCGTGCGAC CGCCCCCTCT GGGGTCCCGG 
GAGTCCTACA GTGACTCCAT GCACAGCTAT GAGGAGTTCT CTGAGCCGCG GGCGCTCAGC 
CCCACAGGCA GCAGCCGCTA TGCTTCTTCC GGGGAGCTGA GCCAGGGAAG CTCACAGCTG 
AGCGAGGACT TCGACCCTGA TGAGCACAGC CTGCAGGGCT CGGAGCTGGA TGACGAGAGG 
GACCGCGATT CTTATCACTC CTGTCACAGC TCCGTGAGTT ACCACAAGGA CTCACCCCGC 
TGGGACCAGG ACGATGAGGA CCTGGAGGAC CTGGAGGACC TGGAAGATGA GGAGCTGCCC 
GAGGAGGAGG AGGAGTTGGA GGAGGAGGGG GAGGAGGAAT TGGAGGAAGA GGATTTGGAG 
GAGGAGGAGG AGGTGCCTGA TGACCTGGCC AGCTACACCC AGCAGGAGGA CACCACTGTG 
GCTGAGCCCA AAGAGTTCAA GCGGATCAGC TTCCCAACGG CTGCGCCTCA GAAGGATGAC 
AAGGTTTCAG CTGTGCCTAC AGAGGCCCCC GAGGTGGCCA AAGGCATCCC CAAAGCAGCC 
ACACCCGAGG AGAAGGCAGC TGCAGAGCGT GCGCAGGAAG CCGAGCCCCC CAAGTCTGAG 
GAGAGTTTCA GATCTCGAGA GGAGGAAGAG GGCCAGGAAG GGCAGGATGC CATGTCCAGA 
GCCAAAGCCA ACTGGTTGCG AGCCTTCAAC AAGGTGCGCA TGCAGCTGCA GGAGGCCCGA 
GGAGAAGGGG ATATGTCTAA GTCTCTATGG TTCAAAGGCG GTCCTGGTGG TGGCCTTATC 
ATCATTGACA GCATGCCAGA CATCAGGAAA CGGAAGCCCA TTCCCCTCGT GAGCGACCTG 
GCTATGTCAT TGGTGCAGTC TCGGAAGGCA GGCATCACCT CGGCCTTGGC CTCCAGCACG 
TTGAACAATG AAGAGCTGAA AAATCACGTT TACAAGAAGA CCCTGCAAGC CTTAATCTAC 
CCCATCTCCT GCACCACGCC GCACAATTTC GAGGTGTGGA CGGCCACCAC GCCCACCTAC 
TGCTATGAGT GCGAGGGGCT GCTGTGGGGC ATCGCGCGGC AGGGCATGCG ATGCACCGAG 
TGCGGCGTTA AGTGCCACGA GAAGTGCCAG GACCTGCTCA ATGCGGATTG CCTGCAGCGG 
GCGGCTGAGA AGAGTTCCAA GCATGGCGCT GAAGACCGCA CGCAGAACAT CATCATGGTG 
CTGAAGGACC GCATGAAGAT CCGCGAGCGC AACAAGCCTG AGATCTTCGA GCTTATCCAA 
GAGATCTTCG CGGTCACCAA GAGCGCACAC ACACAGCAGA TGAAAGCTGT CAAGCAGAGT 
GTGCTGGACG GCACATCCAA GTGGTCTGCC AAAATTAGCA TCACAGTGGT CTGTGCCCAG 
GGCTTGCAGG CCAAGGACAA GACAGGATCC AGTGACCCTT ATGTCACCGT CCAGGTTGGG 
AAGACCAAGA AAAGGACAAA AACCATCTAC GGGAACCTCA ACCCAGTGTG GGAAGAGAAT 
TTTCATTTTG AATGTCACAA CTCCTCTGAC CGGATCAAGG TTCGTGTTTG GGATGAAGAT 
GACGACATAA AATCCCGTGT GAAACAAAGG TTTAAGAGGG AGTCTGATGA CTTCCTAGGG 
CAGACAATCA TCGAGGTGCG GACGCTTAGC GGCGAGATGG ATGTGTGGTA CAATCTGGAC 
AAGAGAACGG ACAAGTCGGC GGTGTCGGGG GCCATCCGGC TTCACATCAG TGTGGAGATC 
AAAGGGGAGG AGAAGGTGGC GCCCTACCAT GTCCAGTACA CCTGTCTGCA TGAGAACCTG 
TTCCACTTTG TGACGGACGT GCAGAACAAT GGTGTGGTGA AGATTCCCGA TGCCAAGGGT 
GACGACGCCT GGAAGGTTTA CTACGATGAG ACGGCCCAGG AGATCGTGGA TGAGTTTGCC 
ATGCGCTATG GTGTTGAGTC CATCTACCAA GCCATGACCC ACTTTGCCTG CCTCTCCTCC 
AAGTATATGT GCCCTGGGGT ACCCGCTGTC ATGAGCACCC TGCTTGCCAA CATCAACGCC 
TACTACGCAC ACACCACCGC CTCCACCAAC GTGTCTGCCT CTGACCGCTT CGCTGCCTCT 
AATTTCGGGA AAGAGCGCTT TGTGAAACTT CTGGACCAGC TACACAATTC TCTGCGGATC 
GACCTGTCCA TGTACCGGAA CAACTTCCCA GCCAGCAGCC CTGAGCGGCT GCAGGATCTC 
AAGTCCACGG TGGACCTGCT CACCAGCATC ACCTTCTTCC GGATGAAGGT TCAGGAACTG 
CAGAGCCCGC CGCGTGCCAG CCAGGTAGTG AAAGACTGTG TGAAGGCCTG CCTCAACTCC 
ACCTATGAGT ACATCTTCAA CAACTGCCAT GAGCTCTATG GCCGGGAGTA CCAAACGGAT 
CCGGCCAAGA AGGGGGAGGT TCCCCCAGAG GAGCAAGGCC CTAGCATCAA GAACCTGGAT 
TTCTGGTCCA AGCTGATCAC CCTCATCGTG TCTATCATCG AGGAGGATAA GAATTCTTAC 
ACACCCTGCC TCAATCAGTT TCCCCAGGAG CTCAATGTGG GGAAGATCAG TGCTGAGGTG 
ATGTGGAGCC TATTTGCCCA AGACATGAAG TACGCCATGG AGGAACATGA CAAACACCGG 
CTGTGTAAGA GCGCAGATTA CATGAACCTG CACTTCAAGG TGAAGTGGCT GTACAATGAG 
TACGTGGCCG AGCTGCCCAC CTTCAAGGAC CGTGTGCCTG AGTACCCTGC GTGGTTTGAG 
CCCTTCGTCA TCCAGTGGTT GGATGAGAAT GAGGAGGTGT CCCGGGACTT CCTGCATGGT 
GCCCTCGAGC GGGACAAGAA GGACGGGTTC CAGCAGACCT CAGAGCACGC CCTGTTCTCT 
TGCTCAGTGG TGGACGTCTT CTCCCAGCTT AACCAGAGTT TCGAGATCAT CAAGAAGCTG 
GAGTGTCCTG ACCCCCAGAT CGTGGGCCAT TACATGCGGC GTTTTGCCAA GACCATTAGC 
AATGTGCTTC TCCAGTATGC AGACATCGTT TCCAAGGACT TCGCCTCCTA CTGCTCCAAG 
GAGAAGGAGA AAGTGCCCTG CATCCTCATG AACAACACAC AGCAACTACG AGTGCAGCTG 
GAGAAGATGT TTGAGGCGAT GGGCGGGAAG GAGCTGGATG CCGAGGCCAG TGGCACCCTG 
AAGGAGCTGC AGGTGAAACT CAACAATGTC CTGGATGAAC TCAGCCATGT GTTTGCCACC 
AGCTTCCAGC CACACATTGA GGAATGCGTC AGACAGATGG GTGACATCCT GAGCCAGGTG 
AAGGGCACAG GCAATGTACC TGCCAGTGCC TGCAGCAGCG TGGCCCAGGA TGCAGACAAT 
GTGCTGCAGC CCATCATGGA TCTTCTGGAC AGCAACCTCA CCCTGTTTGC CAAAATCTGT 
GAGAAGACAG TTCTGAAGCG GGTGCTGAAG GAGCTCTGGA AGCTGGTGAT GAACACAATG 
GAGAAGACCA TCGTCCTGCC ACCGCTCACT GACCAGACGG GGACACAAAT GATCTTCAAT 
GCTGCCAAGG AGCTGGGCCA GCTGTCCAAA CTTAAGGATC ACATGGTGCG GGAAGAAGCC 
AAGAGCTTGA CCCCGAAGCA GTGTGCTGTT GTTGAACTGG CCCTGGACAC CATCAAGCAA 
TACTTCCACG CGGGGGGCGT TGGCCTCAAG AAGACCTTCC TGGAGAAGAG CCCGGACCTC 
CAGTCCCTGC GCTACGCCCT GTCGCTCTAC ACGCAAGCCA CCGACCTGCT CATCAAAACC 
TTCGTGCAGA CGCAGTCAGC GCAGGTCCAT GGTGGAAAAG GGACTAGGTT TACCCTTAGT 
GAAGACGTTT GTCCTGAGAT GGGCTCTGGT GTGGAGGACC CTGTAGGTGA AGTGTCCGTC 
CACGTGGAGC TGTTCACACA TCCAGGAACT GGGGAACAGA AGGTCACAGT GAAGGTGGTG 
GCCGCCAATG ACCTCAAGTG GCAGACTTCT GGCATCTTCC GCCCGTTCAT CGAGGTCAAC 
ATCGTTGGAC CTCAGCTTAG CGACAAGAAA CGCAAGTTCG CCACCAAATC CAAAAACAAC 
AGCTGGGCGC CCAAGTATAA CGAGAGCTTC CAGTTCTCCC TGAGCGCCGA CGCGGGCCCC 
GAGTGCTACG AGCTGCAGGT GTGCGTGAAG GACTACTGCT TTGCGCGCGA AGACCGCACG 
GTGGGGCTGG CGGTGCTGCA ACTGCGAGAG CTGGCCCAGC GCGGGAGCGC CGCGTGCTGG 
CTTCCACTCG GCCGCCGCAT CCACATGGAC GACACGGGGC TCACAGTGCT GCGCATCCTG 
TCGCAGCGCA GCAACGATGA GGTGGCCAAG GAATTCGTCA AGCTCAAGTC GGACACGCGC 
TCAGCCGAGG AGGGCGGTGC CGCGCCTGCG CCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168368.1
CDS	127..5262
Translation	MSLLCVGVKKAKFDGAQEKFNTYVTLKVQNVKSTTIAVRGSQPSWEQDFMFEINRLDLGLTVEVWNKGLIWDTMVGTVWIPLRTIRQSNEEGPGEWLTLDSQAIMADSEICGTKDPTFHRILLDAHFELPLDIPEEEARYWAKKLEQLNAMRDQDEVSHLQYSFQDQQDKPLPVPSSQCCNWNYFGWGEQNDDPDSAVDDRDSDYRSETSNSIPPPYYTTSQPNASVHQYSVRPPPLGSRESYSDSMHSYEEFSEPRALSPTGSSRYASSGELSQGSSQLSEDFDPDEHSLQGSELDDERDRDSYHSCHSSVSYHKDSPRWDQDDEDLEDLEDLEDEELPEEEEELEEEGEEELEEEDLEEEEEVPDDLASYTQQEDTTVAEPKEFKRISFPTAAPQKDDKVSAVPTEAPEVAKGIPKAATPEEKAAAERAQEAEPPKSEESFRSREEEEGQEGQDAMSRAKANWLRAFNKVRMQLQEARGEGDMSKSLWFKGGPGGGLIIIDSMPDIRKRKPIPLVSDLAMSLVQSRKAGITSALASSTLNNEELKNHVYKKTLQALIYPISCTTPHNFEVWTATTPTYCYECEGLLWGIARQGMRCTECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMVLKDRMKIRERNKPEIFELIQEIFAVTKSAHTQQMKAVKQSVLDGTSKWSAKISITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIYGNLNPVWEENFHFECHNSSDRIKVRVWDEDDDIKSRVKQRFKRESDDFLGQTIIEVRTLSGEMDVWYNLDKRTDKSAVSGAIRLHISVEIKGEEKVAPYHVQYTCLHENLFHFVTDVQNNGVVKIPDAKGDDAWKVYYDETAQEIVDEFAMRYGVESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSMYRNNFPASSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHELYGREYQTDPAKKGEVPPEEQGPSIKNLDFWSKLITLIVSIIEEDKNSYTPCLNQFPQELNVGKISAEVMWSLFAQDMKYAMEEHDKHRLCKSADYMNLHFKVKWLYNEYVAELPTFKDRVPEYPAWFEPFVIQWLDENEEVSRDFLHGALERDKKDGFQQTSEHALFSCSVVDVFSQLNQSFEIIKKLECPDPQIVGHYMRRFAKTISNVLLQYADIVSKDFASYCSKEKEKVPCILMNNTQQLRVQLEKMFEAMGGKELDAEASGTLKELQVKLNNVLDELSHVFATSFQPHIEECVRQMGDILSQVKGTGNVPASACSSVAQDADNVLQPIMDLLDSNLTLFAKICEKTVLKRVLKELWKLVMNTMEKTIVLPPLTDQTGTQMIFNAAKELGQLSKLKDHMVREEAKSLTPKQCAVVELALDTIKQYFHAGGVGLKKTFLEKSPDLQSLRYALSLYTQATDLLIKTFVQTQSAQVHGGKGTRFTLSEDVCPEMGSGVEDPVGEVSVHVELFTHPGTGEQKVTVKVVAANDLKWQTSGIFRPFIEVNIVGPQLSDKKRKFATKSKNNSWAPKYNESFQFSLSADAGPECYELQVCVKDYCFAREDRTVGLAVLQLRELAQRGSAACWLPLGRRIHMDDTGLTVLRILSQRSNDEVAKEFVKLKSDTRSAEEGGAAPAP

Target ORF information:

RefSeq Version	XM_017312879.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus unc-13 homolog A (Unc13a), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312879.2

ORF Insert Sequence:

ATGTCTCTGC TGTGCGTGGG AGTCAAAAAA GCCAAGTTTG ACGGTGCCCA AGAGAAGTTC 
AACACATACG TGACGCTGAA GGTGCAGAAC GTGAAGAGCA CTACCATAGC TGTACGTGGC 
AGCCAGCCCA GCTGGGAGCA GGACTTCATG TTTGAGATCA ACCGCCTGGA TCTAGGCCTG 
ACCGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGTAC TGTGTGGATC 
CCGCTGCGGA CCATCCGCCA GTCCAATGAG GAGGGCCCAG GAGAGTGGCT CACACTGGAC 
TCTCAGGCCA TCATGGCGGA CAGTGAGATC TGTGGGACCA AGGACCCCAC CTTCCATCGC 
ATCCTCCTGG ACGCTCATTT CGAGCTGCCG TTGGACATCC CTGAGGAGGA GGCACGCTAC 
TGGGCCAAGA AGCTGGAGCA GCTGAATGCC ATGCGTGACC AGGATGAGGT GAGTCACCTC 
CAGTACTCCT TCCAGGACCA GCAGGACAAG CCGCTGCCAG TGCCCAGCAG CCAGTGCTGC 
AACTGGAATT ACTTTGGCTG GGGAGAACAG AATGACGACC CCGACAGTGC GGTGGATGAC 
CGGGACAGTG ACTATAGGAG TGAGACCAGC AACAGCATCC CACCGCCCTA CTATACGACT 
TCGCAGCCCA ACGCCTCTGT GCACCAGTAC TCCGTGCGAC CGCCCCCTCT GGGGTCCCGG 
GAGTCCTACA GTGACTCCAT GCACAGCTAT GAGGAGTTCT CTGAGCCGCG GGCGCTCAGC 
CCCACAGGCA GCAGCCGCTA TGCTTCTTCC GGGGAGCTGA GCCAGGGAAG CTCACAGCTG 
AGCGAGGACT TCGACCCTGA TGAGCACAGC CTGCAGGGCT CGGAGCTGGA TGACGAGAGG 
GACCGCGATT CTTATCACTC CTGTCACAGC TCCGTGAGTT ACCACAAGGA CTCACCCCGC 
TGGGACCAGG ACGATGAGGA CCTGGAGGAC CTGGAGGACC TGGAAGATGA GGAGCTGCCC 
GAGGAGGAGG AGGAGTTGGA GGAGGAGGGG GAGGAGGAAT TGGAGGAAGA GGATTTGGAG 
GAGGAGGAGG AGGTGCCTGA TGACCTGGCC AGCTACACCC AGCAGGAGGA CACCACTGTG 
GCTGAGCCCA AAGAGTTCAA GCGGATCAGC TTCCCAACGG CTGCGCCTCA GAAGGATGAC 
AAGGTTTCAG CTGTGCCTAC AGAGGCCCCC GAGGTGGCCA AAGGCATCCC CAAAGCAGCC 
ACACCCGAGG AGAAGGCAGC TGCAGAGCGT GCGCAGGAAG CCGAGCCCCC CAAGTCTGAG 
GAGAGTTTCA GATCTCGAGA GGAGGAAGAG GGCCAGGAAG GGCAGGATGC CATGTCCAGA 
GCCAAAGCCA ACTGGTTGCG AGCCTTCAAC AAGGTGCGCA TGCAGCTGCA GGAGGCCCGA 
GGAGAAGGGG ATATGTCTAA GTCTCTATGG TTCAAAGGCG GTCCTGGTGG TGGCCTTATC 
ATCATTGACA GCATGCCAGA CATCAGGAAA CGGAAGCCCA TTCCCCTCGT GAGCGACCTG 
GCTATGTCAT TGGTGCAGTC TCGGAAGGCA GGCATCACCT CGGCCTTGGC CTCCAGCACG 
TTGAACAATG AAGAGCTGAA AAATCACGTT TACAAGAAGA CCCTGCAAGC CTTAATCTAC 
CCCATCTCCT GCACCACGCC GCACAATTTC GAGGTGTGGA CGGCCACCAC GCCCACCTAC 
TGCTATGAGT GCGAGGGGCT GCTGTGGGGC ATCGCGCGGC AGGGCATGCG ATGCACCGAG 
TGCGGCGTTA AGTGCCACGA GAAGTGCCAG GACCTGCTCA ATGCGGATTG CCTGCAGCGG 
GCGGCTGAGA AGAGTTCCAA GCATGGCGCT GAAGACCGCA CGCAGAACAT CATCATGGTG 
CTGAAGGACC GCATGAAGAT CCGCGAGCGC AACAAGCCTG AGATCTTCGA GCTTATCCAA 
GAGATCTTCG CGGTCACCAA GAGCGCACAC ACACAGCAGA TGAAAGCTGT CAAGCAGAGT 
GTGCTGGACG GCACATCCAA GTGGTCTGCC AAAATTAGCA TCACAGTGGT CTGTGCCCAG 
GGCTTGCAGG CCAAGGACAA GACAGGATCC AGTGACCCTT ATGTCACCGT CCAGGTTGGG 
AAGACCAAGA AAAGGACAAA AACCATCTAC GGGAACCTCA ACCCAGTGTG GGAAGAGAAT 
TTTCATTTTG AATGTCACAA CTCCTCTGAC CGGATCAAGG TTCGTGTTTG GGATGAAGAT 
GACGACATAA AATCCCGTGT GAAACAAAGG TTTAAGAGGG AGTCTGATGA CTTCCTAGGG 
CAGACAATCA TCGAGGTGCG GACGCTTAGC GGCGAGATGG ATGTGTGGTA CAATCTGGAC 
AAGAGAACGG ACAAGTCGGC GGTGTCGGGG GCCATCCGGC TTCACATCAG TGTGGAGATC 
AAAGGGGAGG AGAAGGTGGC GCCCTACCAT GTCCAGTACA CCTGTCTGCA TGAGAACCTG 
TTCCACTTTG TGACGGACGT GCAGAACAAT GGTGTGGTGA AGATTCCCGA TGCCAAGGGT 
GACGACGCCT GGAAGGTTTA CTACGATGAG ACGGCCCAGG AGATCGTGGA TGAGTTTGCC 
ATGCGCTATG GTGTTGAGTC CATCTACCAA GCCATGACCC ACTTTGCCTG CCTCTCCTCC 
AAGTATATGT GCCCTGGGGT ACCCGCTGTC ATGAGCACCC TGCTTGCCAA CATCAACGCC 
TACTACGCAC ACACCACCGC CTCCACCAAC GTGTCTGCCT CTGACCGCTT CGCTGCCTCT 
AATTTCGGGA AAGAGCGCTT TGTGAAACTT CTGGACCAGC TACACAATTC TCTGCGGATC 
GACCTGTCCA TGTACCGGAA CAACTTCCCA GCCAGCAGCC CTGAGCGGCT GCAGGATCTC 
AAGTCCACGG TGGACCTGCT CACCAGCATC ACCTTCTTCC GGATGAAGGT TCAGGAACTG 
CAGAGCCCGC CGCGTGCCAG CCAGGTAGTG AAAGACTGTG TGAAGGCCTG CCTCAACTCC 
ACCTATGAGT ACATCTTCAA CAACTGCCAT GAGCTCTATG GCCGGGAGTA CCAAACGGAT 
CCGGCCAAGA AGGGGGAGGT TCCCCCAGAG GAGCAAGGCC CTAGCATCAA GAACCTGGAT 
TTCTGGTCCA AGCTGATCAC CCTCATCGTG TCTATCATCG AGGAGGATAA GAATTCTTAC 
ACACCCTGCC TCAATCAGTT TCCCCAGGAG CTCAATGTGG GGAAGATCAG TGCTGAGGTG 
ATGTGGAGCC TATTTGCCCA AGACATGAAG TACGCCATGG AGGAACATGA CAAACACCGG 
CTGTGTAAGA GCGCAGATTA CATGAACCTG CACTTCAAGG TGAAGTGGCT GTACAATGAG 
TACGTGGCCG AGCTGCCCAC CTTCAAGGAC CGTGTGCCTG AGTACCCTGC GTGGTTTGAG 
CCCTTCGTCA TCCAGTGGTT GGATGAGAAT GAGGAGGTGT CCCGGGACTT CCTGCATGGT 
GCCCTCGAGC GGGACAAGAA GGACGGGTTC CAGCAGACCT CAGAGCACGC CCTGTTCTCT 
TGCTCAGTGG TGGACGTCTT CTCCCAGCTT AACCAGAGTT TCGAGATCAT CAAGAAGCTG 
GAGTGTCCTG ACCCCCAGAT CGTGGGCCAT TACATGCGGC GTTTTGCCAA GACCATTAGC 
AATGTGCTTC TCCAGTATGC AGACATCGTT TCCAAGGACT TCGCCTCCTA CTGCTCCAAG 
GAGAAGGAGA AAGTGCCCTG CATCCTCATG AACAACACAC AGCAACTACG AGTGCAGCTG 
GAGAAGATGT TTGAGGCGAT GGGCGGGAAG GAGCTGGATG CCGAGGCCAG TGGCACCCTG 
AAGGAGCTGC AGGTGAAACT CAACAATGTC CTGGATGAAC TCAGCCATGT GTTTGCCACC 
AGCTTCCAGC CACACATTGA GGAATGCGTC AGACAGATGG GTGACATCCT GAGCCAGGTG 
AAGGGCACAG GCAATGTACC TGCCAGTGCC TGCAGCAGCG TGGCCCAGGA TGCAGACAAT 
GTGCTGCAGC CCATCATGGA TCTTCTGGAC AGCAACCTCA CCCTGTTTGC CAAAATCTGT 
GAGAAGACAG TTCTGAAGCG GGTGCTGAAG GAGCTCTGGA AGCTGGTGAT GAACACAATG 
GAGAAGACCA TCGTCCTGCC ACCGCTCACT GACCAGACGG GGACACAAAT GATCTTCAAT 
GCTGCCAAGG AGCTGGGCCA GCTGTCCAAA CTTAAGGATC ACATGGTGCG GGAAGAAGCC 
AAGAGCTTGA CCCCGAAGCA GTGTGCTGTT GTTGAACTGG CCCTGGACAC CATCAAGCAA 
TACTTCCACG CGGGGGGCGT TGGCCTCAAG AAGACCTTCC TGGAGAAGAG CCCGGACCTC 
CAGTCCCTGC GCTACGCCCT GTCGCTCTAC ACGCAAGCCA CCGACCTGCT CATCAAAACC 
TTCGTGCAGA CGCAGTCAGC GCAGGTCCAT GGTGGAAAAG GGACTAGGTT TACCCTTAGT 
GAAGACGTTT GTCCTGAGAT GGGCTCTGGT GTGGAGGACC CTGTAGGTGA AGTGTCCGTC 
CACGTGGAGC TGTTCACACA TCCAGGAACT GGGGAACAGA AGGTCACAGT GAAGGTGGTG 
GCCGCCAATG ACCTCAAGTG GCAGACTTCT GGCATCTTCC GCCCGTTCAT CGAGGTCAAC 
ATCGTTGGAC CTCAGCTTAG CGACAAGAAA CGCAAGTTCG CCACCAAATC CAAAAACAAC 
AGCTGGGCGC CCAAGTATAA CGAGAGCTTC CAGTTCTCCC TGAGCGCCGA CGCGGGCCCC 
GAGTGCTACG AGCTGCAGGT GTGCGTGAAG GACTACTGCT TTGCGCGCGA AGACCGCACG 
GTGGGGCTGG CGGTGCTGCA ACTGCGAGAG CTGGCCCAGC GCGGGAGCGC CGCGTGCTGG 
CTTCCACTCG GCCGCCGCAT CCACATGGAC GACACGGGGC TCACAGTGCT GCGCATCCTG 
TCGCAGCGCA GCAACGATGA GGTGGCCAAG GAATTCGTCA AGCTCAAGTC GGACACGCGC 
TCAGCCGAGG AGGGCGGTGC CGCGCCTGCG CCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Unc13a ( XM_017312880.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168369.1 Mus musculus unc-13 homolog A (Unc13a), transcript variant X9, mRNA.

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CloneID	OMu82530
Clone ID Related Accession (Same CDS sequence)	XM_017312880.2 , XM_017312880.1
Accession Version	XM_017312880.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5085bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein unc-13 homolog A isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017312880.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTGC TGTGCGTGGG AGTCAAAAAA GCCAAGTTTG ACGGTGCCCA AGAGAAGTTC 
AACACATACG TGACGCTGAA GGTGCAGAAC GTGAAGAGCA CTACCATAGC TGTACGTGGC 
AGCCAGCCCA GCTGGGAGCA GGACTTCATG TTTGAGATCA ACCGCCTGGA TCTAGGCCTG 
ACCGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGTAC TGTGTGGATC 
CCGCTGCGGA CCATCCGCCA GTCCAATGAG GAGGGCCCAG GAGAGTGGCT CACACTGGAC 
TCTCAGGCCA TCATGGCGGA CAGTGAGATC TGTGGGACCA AGGACCCCAC CTTCCATCGC 
ATCCTCCTGG ACGCTCATTT CGAGCTGCCG TTGGACATCC CTGAGGAGGA GGCACGCTAC 
TGGGCCAAGA AGCTGGAGCA GCTGAATGCC ATGCGTGACC AGGATGAGGT GAGTCACCTC 
CAGTACTCCT TCCAGGACCA GCAGGACAAG CCGCTGCCAG TGCCCAGCAG CCAGTGCTGC 
AACTGGAATT ACTTTGGCTG GGGAGAACAG AATGACGACC CCGACAGTGC GGTGGATGAC 
CGGGACAGTG ACTATAGGAG TGAGACCAGC AACAGCATCC CACCGCCCTA CTATACGACT 
TCGCAGCCCA ACGCCTCTGT GCACCAGTAC TCCGTGCGAC CGCCCCCTCT GGGGTCCCGG 
GAGTCCTACA GTGACTCCAT GCACAGCTAT GAGGAGTTCT CTGAGCCGCG GGCGCTCAGC 
CCCACAGGCA GCAGCCGCTA TGCTTCTTCC GGGGAGCTGA GCCAGGGAAG CTCACAGCTG 
AGCGAGGACT TCGACCCTGA TGAGCACAGC CTGCAGGGCT CGGAGCTGGA TGACGAGAGG 
GACCGCGATT CTTATCACTC CTGTCACAGC TCCGTGAGTT ACCACAAGGA CTCACCCCGC 
TGGGACCAGG ACGATGAGGA CCTGGAGGAC CTGGAGGACC TGGAAGATGA GGAGCTGCCC 
GAGGAGGAGG AGGAGTTGGA GGAGGAGGGG GAGGAGGAAT TGGAGGAAGA GGATTTGGAG 
GAGGAGGAGG AGGTGCCTGA TGACCTGGCC AGCTACACCC AGCAGGAGGA CACCACTGTG 
GCTGAGCCCA AAGAGTTCAA GCGGATCAGC TTCCCAACGG CTGCGCCTCA GAAGGATGAC 
AAGGTTTCAG CTGTGCCTAC AGAGGCCCCC GAGGTGGCCA AAGGCATCCC CAAAGCAGCC 
ACACCCGAGG AGAAGGCAGC TGCAGAGCGT GCGCAGGAAG CCGAGCCCCC CAAGTCTGAG 
GAGAGTTTCA GATCTCGAGA GGAGGAAGAG GGCCAGGAAG GGCAGGATGC CATGTCCAGA 
GCCAAAGCCA ACTGGTTGCG AGCCTTCAAC AAGGTGCGCA TGCAGCTGCA GGAGGCCCGA 
GGAGAAGGGG ATATGTCTAA GTCTCTATGG TTCAAAGGCG GTCCTGGTGG TGGCCTTATC 
ATCATTGACA GCATGCCAGA CATCAGGAAA CGGAAGCCCA TTCCCCTCGT GAGCGACCTG 
GCTATGTCAT TGGTGCAGTC TCGGAAGGCA GGCATCACCT CGGCCTTGGC CTCCAGCACG 
TTGAACAATG AAGAGCTGAA AAATCACGTT TACAAGAAGA CCCTGCAAGC CTTAATCTAC 
CCCATCTCCT GCACCACGCC GCACAATTTC GAGGTGTGGA CGGCCACCAC GCCCACCTAC 
TGCTATGAGT GCGAGGGGCT GCTGTGGGGC ATCGCGCGGC AGGGCATGCG ATGCACCGAG 
TGCGGCGTTA AGTGCCACGA GAAGTGCCAG GACCTGCTCA ATGCGGATTG CCTGCAGCGG 
GCGGCTGAGA AGAGTTCCAA GCATGGCGCT GAAGACCGCA CGCAGAACAT CATCATGGTG 
CTGAAGGACC GCATGAAGAT CCGCGAGCGC AACAAGCCTG AGATCTTCGA GCTTATCCAA 
GAGATCTTCG CGGTCACCAA GAGCGCACAC ACACAGCAGA TGAAAGCTGT CAAGCAGAGT 
GTGCTGGACG GCACATCCAA GTGGTCTGCC AAAATTAGCA TCACAGTGGT CTGTGCCCAG 
GGCTTGCAGG CCAAGGACAA GACAGGATCC AGTGACCCTT ATGTCACCGT CCAGGTTGGG 
AAGACCAAGA AAAGGACAAA AACCATCTAC GGGAACCTCA ACCCAGTGTG GGAAGAGAAT 
TTTCATTTTG AATGTCACAA CTCCTCTGAC CGGATCAAGG TTCGTGTTTG GGATGAAGAT 
GACGACATAA AATCCCGTGT GAAACAAAGG TTTAAGAGGG AGTCTGATGA CTTCCTAGGG 
CAGACAATCA TCGAGGTGCG GACGCTTAGC GGCGAGATGG ATGTGTGGTA CAATCTGGAC 
AAGAGAACGG ACAAGTCGGC GGTGTCGGGG GCCATCCGGC TTCACATCAG TGTGGAGATC 
AAAGGGGAGG AGAAGGTGGC GCCCTACCAT GTCCAGTACA CCTGTCTGCA TGAGAACCTG 
TTCCACTTTG TGACGGACGT GCAGAACAAT GGTGTGGTGA AGATTCCCGA TGCCAAGGGT 
GACGACGCCT GGAAGGTTTA CTACGATGAG ACGGCCCAGG AGATCGTGGA TGAGTTTGCC 
ATGCGCTATG GTGTTGAGTC CATCTACCAA GCCATGACCC ACTTTGCCTG CCTCTCCTCC 
AAGTATATGT GCCCTGGGGT ACCCGCTGTC ATGAGCACCC TGCTTGCCAA CATCAACGCC 
TACTACGCAC ACACCACCGC CTCCACCAAC GTGTCTGCCT CTGACCGCTT CGCTGCCTCT 
AATTTCGGGA AAGAGCGCTT TGTGAAACTT CTGGACCAGC TACACAATTC TCTGCGGATC 
GACCTGTCCA TGTACCGGAA CAACTTCCCA GCCAGCAGCC CTGAGCGGCT GCAGGATCTC 
AAGTCCACGG TGGACCTGCT CACCAGCATC ACCTTCTTCC GGATGAAGGT TCAGGAACTG 
CAGAGCCCGC CGCGTGCCAG CCAGGTAGTG AAAGACTGTG TGAAGGCCTG CCTCAACTCC 
ACCTATGAGT ACATCTTCAA CAACTGCCAT GAGCTCTATG GCCGGGAGTA CCAAACGGAT 
CCGGCCAAGA AGGGGGAGGT TCCCCCAGAG GAGCAAGGCC CTAGCATCAA GAACCTGGAT 
TTCTGGTCCA AGCTGATCAC CCTCATCGTG TCTATCATCG AGGAGGATAA GAATTCTTAC 
ACACCCTGCC TCAATCAGTT TCCCCAGGAG CTCAATGTGG GGAAGATCAG TGCTGAGGTG 
ATGTGGAGCC TATTTGCCCA AGACATGAAG TACGCCATGG AGGAACATGA CAAACACCGG 
CTGTGTAAGA GCGCAGATTA CATGAACCTG CACTTCAAGG TGAAGTGGCT GTACAATGAG 
TACGTGGCCG AGCTGCCCAC CTTCAAGGAC CGTGTGCCTG AGTACCCTGC GTGGTTTGAG 
CCCTTCGTCA TCCAGTGGTT GGATGAGAAT GAGGAGGTGT CCCGGGACTT CCTGCATGGT 
GCCCTCGAGC GGGACAAGAA GGACGGGTTC CAGCAGACCT CAGAGCACGC CCTGTTCTCT 
TGCTCAGTGG TGGACGTCTT CTCCCAGCTT AACCAGAGTT TCGAGATCAT CAAGAAGCTG 
GAGTGTCCTG ACCCCCAGAT CGTGGGCCAT TACATGCGGC GTTTTGCCAA GACCATTAGC 
AATGTGCTTC TCCAGTATGC AGACATCGTT TCCAAGGACT TCGCCTCCTA CTGCTCCAAG 
GAGAAGGAGA AAGTGCCCTG CATCCTCATG AACAACACAC AGCAACTACG AGTGCAGCTG 
GAGAAGATGT TTGAGGCGAT GGGCGGGAAG GAGCTGGATG CCGAGGCCAG TGGCACCCTG 
AAGGAGCTGC AGGTGAAACT CAACAATGTC CTGGATGAAC TCAGCCATGT GTTTGCCACC 
AGCTTCCAGC CACACATTGA GGAATGCGTC AGACAGATGG GTGACATCCT GAGCCAGGTG 
AAGGGCACAG GCAATGTACC TGCCAGTGCC TGCAGCAGCG TGGCCCAGGA TGCAGACAAT 
GTGCTGCAGC CCATCATGGA TCTTCTGGAC AGCAACCTCA CCCTGTTTGC CAAAATCTGT 
GAGAAGACAG TTCTGAAGCG GGTGCTGAAG GAGCTCTGGA AGCTGGTGAT GAACACAATG 
GAGAAGACCA TCGTCCTGCC ACCGCTCACT GACCAGACGA TGATTGGGAC ACAAATGATC 
TTCAATGCTG CCAAGGAGCT GGGCCAGCTG TCCAAACTTA AGGATCACAT GGTGCGGGAA 
GAAGCCAAGA GCTTGACCCC GAAGCAGTGT GCTGTTGTTG AACTGGCCCT GGACACCATC 
AAGCAATACT TCCACGCGGG GGGCGTTGGC CTCAAGAAGA CCTTCCTGGA GAAGAGCCCG 
GACCTCCAGT CCCTGCGCTA CGCCCTGTCG CTCTACACGC AAGCCACCGA CCTGCTCATC 
AAAACCTTCG TGCAGACGCA GTCAGCGCAG GGCTCTGGTG TGGAGGACCC TGTAGGTGAA 
GTGTCCGTCC ACGTGGAGCT GTTCACACAT CCAGGAACTG GGGAACAGAA GGTCACAGTG 
AAGGTGGTGG CCGCCAATGA CCTCAAGTGG CAGACTTCTG GCATCTTCCG CCCGTTCATC 
GAGGTCAACA TCGTTGGACC TCAGCTTAGC GACAAGAAAC GCAAGTTCGC CACCAAATCC 
AAAAACAACA GCTGGGCGCC CAAGTATAAC GAGAGCTTCC AGTTCTCCCT GAGCGCCGAC 
GCGGGCCCCG AGTGCTACGA GCTGCAGGTG TGCGTGAAGG ACTACTGCTT TGCGCGCGAA 
GACCGCACGG TGGGGCTGGC GGTGCTGCAA CTGCGAGAGC TGGCCCAGCG CGGGAGCGCC 
GCGTGCTGGC TTCCACTCGG CCGCCGCATC CACATGGACG ACACGGGGCT CACAGTGCTG 
CGCATCCTGT CGCAGCGCAG CAACGATGAG GTGGCCAAGG AATTCGTCAA GCTCAAGTCG 
GACACGCGCT CAGCCGAGGA GGGCGGTGCC GCGCCTGCGC CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168369.1
CDS	126..5210
Translation	MSLLCVGVKKAKFDGAQEKFNTYVTLKVQNVKSTTIAVRGSQPSWEQDFMFEINRLDLGLTVEVWNKGLIWDTMVGTVWIPLRTIRQSNEEGPGEWLTLDSQAIMADSEICGTKDPTFHRILLDAHFELPLDIPEEEARYWAKKLEQLNAMRDQDEVSHLQYSFQDQQDKPLPVPSSQCCNWNYFGWGEQNDDPDSAVDDRDSDYRSETSNSIPPPYYTTSQPNASVHQYSVRPPPLGSRESYSDSMHSYEEFSEPRALSPTGSSRYASSGELSQGSSQLSEDFDPDEHSLQGSELDDERDRDSYHSCHSSVSYHKDSPRWDQDDEDLEDLEDLEDEELPEEEEELEEEGEEELEEEDLEEEEEVPDDLASYTQQEDTTVAEPKEFKRISFPTAAPQKDDKVSAVPTEAPEVAKGIPKAATPEEKAAAERAQEAEPPKSEESFRSREEEEGQEGQDAMSRAKANWLRAFNKVRMQLQEARGEGDMSKSLWFKGGPGGGLIIIDSMPDIRKRKPIPLVSDLAMSLVQSRKAGITSALASSTLNNEELKNHVYKKTLQALIYPISCTTPHNFEVWTATTPTYCYECEGLLWGIARQGMRCTECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMVLKDRMKIRERNKPEIFELIQEIFAVTKSAHTQQMKAVKQSVLDGTSKWSAKISITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIYGNLNPVWEENFHFECHNSSDRIKVRVWDEDDDIKSRVKQRFKRESDDFLGQTIIEVRTLSGEMDVWYNLDKRTDKSAVSGAIRLHISVEIKGEEKVAPYHVQYTCLHENLFHFVTDVQNNGVVKIPDAKGDDAWKVYYDETAQEIVDEFAMRYGVESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSMYRNNFPASSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHELYGREYQTDPAKKGEVPPEEQGPSIKNLDFWSKLITLIVSIIEEDKNSYTPCLNQFPQELNVGKISAEVMWSLFAQDMKYAMEEHDKHRLCKSADYMNLHFKVKWLYNEYVAELPTFKDRVPEYPAWFEPFVIQWLDENEEVSRDFLHGALERDKKDGFQQTSEHALFSCSVVDVFSQLNQSFEIIKKLECPDPQIVGHYMRRFAKTISNVLLQYADIVSKDFASYCSKEKEKVPCILMNNTQQLRVQLEKMFEAMGGKELDAEASGTLKELQVKLNNVLDELSHVFATSFQPHIEECVRQMGDILSQVKGTGNVPASACSSVAQDADNVLQPIMDLLDSNLTLFAKICEKTVLKRVLKELWKLVMNTMEKTIVLPPLTDQTMIGTQMIFNAAKELGQLSKLKDHMVREEAKSLTPKQCAVVELALDTIKQYFHAGGVGLKKTFLEKSPDLQSLRYALSLYTQATDLLIKTFVQTQSAQGSGVEDPVGEVSVHVELFTHPGTGEQKVTVKVVAANDLKWQTSGIFRPFIEVNIVGPQLSDKKRKFATKSKNNSWAPKYNESFQFSLSADAGPECYELQVCVKDYCFAREDRTVGLAVLQLRELAQRGSAACWLPLGRRIHMDDTGLTVLRILSQRSNDEVAKEFVKLKSDTRSAEEGGAAPAP

Target ORF information:

RefSeq Version	XM_017312880.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus unc-13 homolog A (Unc13a), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312880.2

ORF Insert Sequence:

ATGTCTCTGC TGTGCGTGGG AGTCAAAAAA GCCAAGTTTG ACGGTGCCCA AGAGAAGTTC 
AACACATACG TGACGCTGAA GGTGCAGAAC GTGAAGAGCA CTACCATAGC TGTACGTGGC 
AGCCAGCCCA GCTGGGAGCA GGACTTCATG TTTGAGATCA ACCGCCTGGA TCTAGGCCTG 
ACCGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGTAC TGTGTGGATC 
CCGCTGCGGA CCATCCGCCA GTCCAATGAG GAGGGCCCAG GAGAGTGGCT CACACTGGAC 
TCTCAGGCCA TCATGGCGGA CAGTGAGATC TGTGGGACCA AGGACCCCAC CTTCCATCGC 
ATCCTCCTGG ACGCTCATTT CGAGCTGCCG TTGGACATCC CTGAGGAGGA GGCACGCTAC 
TGGGCCAAGA AGCTGGAGCA GCTGAATGCC ATGCGTGACC AGGATGAGGT GAGTCACCTC 
CAGTACTCCT TCCAGGACCA GCAGGACAAG CCGCTGCCAG TGCCCAGCAG CCAGTGCTGC 
AACTGGAATT ACTTTGGCTG GGGAGAACAG AATGACGACC CCGACAGTGC GGTGGATGAC 
CGGGACAGTG ACTATAGGAG TGAGACCAGC AACAGCATCC CACCGCCCTA CTATACGACT 
TCGCAGCCCA ACGCCTCTGT GCACCAGTAC TCCGTGCGAC CGCCCCCTCT GGGGTCCCGG 
GAGTCCTACA GTGACTCCAT GCACAGCTAT GAGGAGTTCT CTGAGCCGCG GGCGCTCAGC 
CCCACAGGCA GCAGCCGCTA TGCTTCTTCC GGGGAGCTGA GCCAGGGAAG CTCACAGCTG 
AGCGAGGACT TCGACCCTGA TGAGCACAGC CTGCAGGGCT CGGAGCTGGA TGACGAGAGG 
GACCGCGATT CTTATCACTC CTGTCACAGC TCCGTGAGTT ACCACAAGGA CTCACCCCGC 
TGGGACCAGG ACGATGAGGA CCTGGAGGAC CTGGAGGACC TGGAAGATGA GGAGCTGCCC 
GAGGAGGAGG AGGAGTTGGA GGAGGAGGGG GAGGAGGAAT TGGAGGAAGA GGATTTGGAG 
GAGGAGGAGG AGGTGCCTGA TGACCTGGCC AGCTACACCC AGCAGGAGGA CACCACTGTG 
GCTGAGCCCA AAGAGTTCAA GCGGATCAGC TTCCCAACGG CTGCGCCTCA GAAGGATGAC 
AAGGTTTCAG CTGTGCCTAC AGAGGCCCCC GAGGTGGCCA AAGGCATCCC CAAAGCAGCC 
ACACCCGAGG AGAAGGCAGC TGCAGAGCGT GCGCAGGAAG CCGAGCCCCC CAAGTCTGAG 
GAGAGTTTCA GATCTCGAGA GGAGGAAGAG GGCCAGGAAG GGCAGGATGC CATGTCCAGA 
GCCAAAGCCA ACTGGTTGCG AGCCTTCAAC AAGGTGCGCA TGCAGCTGCA GGAGGCCCGA 
GGAGAAGGGG ATATGTCTAA GTCTCTATGG TTCAAAGGCG GTCCTGGTGG TGGCCTTATC 
ATCATTGACA GCATGCCAGA CATCAGGAAA CGGAAGCCCA TTCCCCTCGT GAGCGACCTG 
GCTATGTCAT TGGTGCAGTC TCGGAAGGCA GGCATCACCT CGGCCTTGGC CTCCAGCACG 
TTGAACAATG AAGAGCTGAA AAATCACGTT TACAAGAAGA CCCTGCAAGC CTTAATCTAC 
CCCATCTCCT GCACCACGCC GCACAATTTC GAGGTGTGGA CGGCCACCAC GCCCACCTAC 
TGCTATGAGT GCGAGGGGCT GCTGTGGGGC ATCGCGCGGC AGGGCATGCG ATGCACCGAG 
TGCGGCGTTA AGTGCCACGA GAAGTGCCAG GACCTGCTCA ATGCGGATTG CCTGCAGCGG 
GCGGCTGAGA AGAGTTCCAA GCATGGCGCT GAAGACCGCA CGCAGAACAT CATCATGGTG 
CTGAAGGACC GCATGAAGAT CCGCGAGCGC AACAAGCCTG AGATCTTCGA GCTTATCCAA 
GAGATCTTCG CGGTCACCAA GAGCGCACAC ACACAGCAGA TGAAAGCTGT CAAGCAGAGT 
GTGCTGGACG GCACATCCAA GTGGTCTGCC AAAATTAGCA TCACAGTGGT CTGTGCCCAG 
GGCTTGCAGG CCAAGGACAA GACAGGATCC AGTGACCCTT ATGTCACCGT CCAGGTTGGG 
AAGACCAAGA AAAGGACAAA AACCATCTAC GGGAACCTCA ACCCAGTGTG GGAAGAGAAT 
TTTCATTTTG AATGTCACAA CTCCTCTGAC CGGATCAAGG TTCGTGTTTG GGATGAAGAT 
GACGACATAA AATCCCGTGT GAAACAAAGG TTTAAGAGGG AGTCTGATGA CTTCCTAGGG 
CAGACAATCA TCGAGGTGCG GACGCTTAGC GGCGAGATGG ATGTGTGGTA CAATCTGGAC 
AAGAGAACGG ACAAGTCGGC GGTGTCGGGG GCCATCCGGC TTCACATCAG TGTGGAGATC 
AAAGGGGAGG AGAAGGTGGC GCCCTACCAT GTCCAGTACA CCTGTCTGCA TGAGAACCTG 
TTCCACTTTG TGACGGACGT GCAGAACAAT GGTGTGGTGA AGATTCCCGA TGCCAAGGGT 
GACGACGCCT GGAAGGTTTA CTACGATGAG ACGGCCCAGG AGATCGTGGA TGAGTTTGCC 
ATGCGCTATG GTGTTGAGTC CATCTACCAA GCCATGACCC ACTTTGCCTG CCTCTCCTCC 
AAGTATATGT GCCCTGGGGT ACCCGCTGTC ATGAGCACCC TGCTTGCCAA CATCAACGCC 
TACTACGCAC ACACCACCGC CTCCACCAAC GTGTCTGCCT CTGACCGCTT CGCTGCCTCT 
AATTTCGGGA AAGAGCGCTT TGTGAAACTT CTGGACCAGC TACACAATTC TCTGCGGATC 
GACCTGTCCA TGTACCGGAA CAACTTCCCA GCCAGCAGCC CTGAGCGGCT GCAGGATCTC 
AAGTCCACGG TGGACCTGCT CACCAGCATC ACCTTCTTCC GGATGAAGGT TCAGGAACTG 
CAGAGCCCGC CGCGTGCCAG CCAGGTAGTG AAAGACTGTG TGAAGGCCTG CCTCAACTCC 
ACCTATGAGT ACATCTTCAA CAACTGCCAT GAGCTCTATG GCCGGGAGTA CCAAACGGAT 
CCGGCCAAGA AGGGGGAGGT TCCCCCAGAG GAGCAAGGCC CTAGCATCAA GAACCTGGAT 
TTCTGGTCCA AGCTGATCAC CCTCATCGTG TCTATCATCG AGGAGGATAA GAATTCTTAC 
ACACCCTGCC TCAATCAGTT TCCCCAGGAG CTCAATGTGG GGAAGATCAG TGCTGAGGTG 
ATGTGGAGCC TATTTGCCCA AGACATGAAG TACGCCATGG AGGAACATGA CAAACACCGG 
CTGTGTAAGA GCGCAGATTA CATGAACCTG CACTTCAAGG TGAAGTGGCT GTACAATGAG 
TACGTGGCCG AGCTGCCCAC CTTCAAGGAC CGTGTGCCTG AGTACCCTGC GTGGTTTGAG 
CCCTTCGTCA TCCAGTGGTT GGATGAGAAT GAGGAGGTGT CCCGGGACTT CCTGCATGGT 
GCCCTCGAGC GGGACAAGAA GGACGGGTTC CAGCAGACCT CAGAGCACGC CCTGTTCTCT 
TGCTCAGTGG TGGACGTCTT CTCCCAGCTT AACCAGAGTT TCGAGATCAT CAAGAAGCTG 
GAGTGTCCTG ACCCCCAGAT CGTGGGCCAT TACATGCGGC GTTTTGCCAA GACCATTAGC 
AATGTGCTTC TCCAGTATGC AGACATCGTT TCCAAGGACT TCGCCTCCTA CTGCTCCAAG 
GAGAAGGAGA AAGTGCCCTG CATCCTCATG AACAACACAC AGCAACTACG AGTGCAGCTG 
GAGAAGATGT TTGAGGCGAT GGGCGGGAAG GAGCTGGATG CCGAGGCCAG TGGCACCCTG 
AAGGAGCTGC AGGTGAAACT CAACAATGTC CTGGATGAAC TCAGCCATGT GTTTGCCACC 
AGCTTCCAGC CACACATTGA GGAATGCGTC AGACAGATGG GTGACATCCT GAGCCAGGTG 
AAGGGCACAG GCAATGTACC TGCCAGTGCC TGCAGCAGCG TGGCCCAGGA TGCAGACAAT 
GTGCTGCAGC CCATCATGGA TCTTCTGGAC AGCAACCTCA CCCTGTTTGC CAAAATCTGT 
GAGAAGACAG TTCTGAAGCG GGTGCTGAAG GAGCTCTGGA AGCTGGTGAT GAACACAATG 
GAGAAGACCA TCGTCCTGCC ACCGCTCACT GACCAGACGA TGATTGGGAC ACAAATGATC 
TTCAATGCTG CCAAGGAGCT GGGCCAGCTG TCCAAACTTA AGGATCACAT GGTGCGGGAA 
GAAGCCAAGA GCTTGACCCC GAAGCAGTGT GCTGTTGTTG AACTGGCCCT GGACACCATC 
AAGCAATACT TCCACGCGGG GGGCGTTGGC CTCAAGAAGA CCTTCCTGGA GAAGAGCCCG 
GACCTCCAGT CCCTGCGCTA CGCCCTGTCG CTCTACACGC AAGCCACCGA CCTGCTCATC 
AAAACCTTCG TGCAGACGCA GTCAGCGCAG GGCTCTGGTG TGGAGGACCC TGTAGGTGAA 
GTGTCCGTCC ACGTGGAGCT GTTCACACAT CCAGGAACTG GGGAACAGAA GGTCACAGTG 
AAGGTGGTGG CCGCCAATGA CCTCAAGTGG CAGACTTCTG GCATCTTCCG CCCGTTCATC 
GAGGTCAACA TCGTTGGACC TCAGCTTAGC GACAAGAAAC GCAAGTTCGC CACCAAATCC 
AAAAACAACA GCTGGGCGCC CAAGTATAAC GAGAGCTTCC AGTTCTCCCT GAGCGCCGAC 
GCGGGCCCCG AGTGCTACGA GCTGCAGGTG TGCGTGAAGG ACTACTGCTT TGCGCGCGAA 
GACCGCACGG TGGGGCTGGC GGTGCTGCAA CTGCGAGAGC TGGCCCAGCG CGGGAGCGCC 
GCGTGCTGGC TTCCACTCGG CCGCCGCATC CACATGGACG ACACGGGGCT CACAGTGCTG 
CGCATCCTGT CGCAGCGCAG CAACGATGAG GTGGCCAAGG AATTCGTCAA GCTCAAGTCG 
GACACGCGCT CAGCCGAGGA GGGCGGTGCC GCGCCTGCGC CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Unc13a ( XM_017312881.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168370.1 Mus musculus unc-13 homolog A (Unc13a), transcript variant X10, mRNA.

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CloneID	OMu82531
Clone ID Related Accession (Same CDS sequence)	XM_017312881.2 , XM_017312881.1
Accession Version	XM_017312881.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5079bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein unc-13 homolog A isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017312881.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTGC TGTGCGTGGG AGTCAAAAAA GCCAAGTTTG ACGGTGCCCA AGAGAAGTTC 
AACACATACG TGACGCTGAA GGTGCAGAAC GTGAAGAGCA CTACCATAGC TGTACGTGGC 
AGCCAGCCCA GCTGGGAGCA GGACTTCATG TTTGAGATCA ACCGCCTGGA TCTAGGCCTG 
ACCGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGTAC TGTGTGGATC 
CCGCTGCGGA CCATCCGCCA GTCCAATGAG GAGGGCCCAG GAGAGTGGCT CACACTGGAC 
TCTCAGGCCA TCATGGCGGA CAGTGAGATC TGTGGGACCA AGGACCCCAC CTTCCATCGC 
ATCCTCCTGG ACGCTCATTT CGAGCTGCCG TTGGACATCC CTGAGGAGGA GGCACGCTAC 
TGGGCCAAGA AGCTGGAGCA GCTGAATGCC ATGCGTGACC AGGATGAGGT GAGTCACCTC 
CAGTACTCCT TCCAGGACCA GCAGGACAAG CCGCTGCCAG TGCCCAGCAG CCAGTGCTGC 
AACTGGAATT ACTTTGGCTG GGGAGAACAG AATGACGACC CCGACAGTGC GGTGGATGAC 
CGGGACAGTG ACTATAGGAG TGAGACCAGC AACAGCATCC CACCGCCCTA CTATACGACT 
TCGCAGCCCA ACGCCTCTGT GCACCAGTAC TCCGTGCGAC CGCCCCCTCT GGGGTCCCGG 
GAGTCCTACA GTGACTCCAT GCACAGCTAT GAGGAGTTCT CTGAGCCGCG GGCGCTCAGC 
CCCACAGGCA GCAGCCGCTA TGCTTCTTCC GGGGAGCTGA GCCAGGGAAG CTCACAGCTG 
AGCGAGGACT TCGACCCTGA TGAGCACAGC CTGCAGGGCT CGGAGCTGGA TGACGAGAGG 
GACCGCGATT CTTATCACTC CTGTCACAGC TCCGTGAGTT ACCACAAGGA CTCACCCCGC 
TGGGACCAGG ACGATGAGGA CCTGGAGGAC CTGGAGGACC TGGAAGATGA GGAGCTGCCC 
GAGGAGGAGG AGGAGTTGGA GGAGGAGGGG GAGGAGGAAT TGGAGGAAGA GGATTTGGAG 
GAGGAGGAGG AGGTGCCTGA TGACCTGGCC AGCTACACCC AGCAGGAGGA CACCACTGTG 
GCTGAGCCCA AAGAGTTCAA GCGGATCAGC TTCCCAACGG CTGCGCCTCA GAAGGATGAC 
AAGGTTTCAG CTGTGCCTAC AGAGGCCCCC GAGGTGGCCA AAGGCATCCC CAAAGCAGCC 
ACACCCGAGG AGAAGGCAGC TGCAGAGCGT GCGCAGGAAG CCGAGCCCCC CAAGTCTGAG 
GAGAGTTTCA GATCTCGAGA GGAGGAAGAG GGCCAGGAAG GGCAGGATGC CATGTCCAGA 
GCCAAAGCCA ACTGGTTGCG AGCCTTCAAC AAGGTGCGCA TGCAGCTGCA GGAGGCCCGA 
GGAGAAGGGG ATATGTCTAA GTCTCTATGG TTCAAAGGCG GTCCTGGTGG TGGCCTTATC 
ATCATTGACA GCATGCCAGA CATCAGGAAA CGGAAGCCCA TTCCCCTCGT GAGCGACCTG 
GCTATGTCAT TGGTGCAGTC TCGGAAGGCA GGCATCACCT CGGCCTTGGC CTCCAGCACG 
TTGAACAATG AAGAGCTGAA AAATCACGTT TACAAGAAGA CCCTGCAAGC CTTAATCTAC 
CCCATCTCCT GCACCACGCC GCACAATTTC GAGGTGTGGA CGGCCACCAC GCCCACCTAC 
TGCTATGAGT GCGAGGGGCT GCTGTGGGGC ATCGCGCGGC AGGGCATGCG ATGCACCGAG 
TGCGGCGTTA AGTGCCACGA GAAGTGCCAG GACCTGCTCA ATGCGGATTG CCTGCAGCGG 
GCGGCTGAGA AGAGTTCCAA GCATGGCGCT GAAGACCGCA CGCAGAACAT CATCATGGTG 
CTGAAGGACC GCATGAAGAT CCGCGAGCGC AACAAGCCTG AGATCTTCGA GCTTATCCAA 
GAGATCTTCG CGGTCACCAA GAGCGCACAC ACACAGCAGA TGAAAGCTGT CAAGCAGAGT 
GTGCTGGACG GCACATCCAA GTGGTCTGCC AAAATTAGCA TCACAGTGGT CTGTGCCCAG 
GGCTTGCAGG CCAAGGACAA GACAGGATCC AGTGACCCTT ATGTCACCGT CCAGGTTGGG 
AAGACCAAGA AAAGGACAAA AACCATCTAC GGGAACCTCA ACCCAGTGTG GGAAGAGAAT 
TTTCATTTTG AATGTCACAA CTCCTCTGAC CGGATCAAGG TTCGTGTTTG GGATGAAGAT 
GACGACATAA AATCCCGTGT GAAACAAAGG TTTAAGAGGG AGTCTGATGA CTTCCTAGGG 
CAGACAATCA TCGAGGTGCG GACGCTTAGC GGCGAGATGG ATGTGTGGTA CAATCTGGAC 
AAGAGAACGG ACAAGTCGGC GGTGTCGGGG GCCATCCGGC TTCACATCAG TGTGGAGATC 
AAAGGGGAGG AGAAGGTGGC GCCCTACCAT GTCCAGTACA CCTGTCTGCA TGAGAACCTG 
TTCCACTTTG TGACGGACGT GCAGAACAAT GGTGTGGTGA AGATTCCCGA TGCCAAGGGT 
GACGACGCCT GGAAGGTTTA CTACGATGAG ACGGCCCAGG AGATCGTGGA TGAGTTTGCC 
ATGCGCTATG GTGTTGAGTC CATCTACCAA GCCATGACCC ACTTTGCCTG CCTCTCCTCC 
AAGTATATGT GCCCTGGGGT ACCCGCTGTC ATGAGCACCC TGCTTGCCAA CATCAACGCC 
TACTACGCAC ACACCACCGC CTCCACCAAC GTGTCTGCCT CTGACCGCTT CGCTGCCTCT 
AATTTCGGGA AAGAGCGCTT TGTGAAACTT CTGGACCAGC TACACAATTC TCTGCGGATC 
GACCTGTCCA TGTACCGGAA CAACTTCCCA GCCAGCAGCC CTGAGCGGCT GCAGGATCTC 
AAGTCCACGG TGGACCTGCT CACCAGCATC ACCTTCTTCC GGATGAAGGT TCAGGAACTG 
CAGAGCCCGC CGCGTGCCAG CCAGGTAGTG AAAGACTGTG TGAAGGCCTG CCTCAACTCC 
ACCTATGAGT ACATCTTCAA CAACTGCCAT GAGCTCTATG GCCGGGAGTA CCAAACGGAT 
CCGGCCAAGA AGGGGGAGGT TCCCCCAGAG GAGCAAGGCC CTAGCATCAA GAACCTGGAT 
TTCTGGTCCA AGCTGATCAC CCTCATCGTG TCTATCATCG AGGAGGATAA GAATTCTTAC 
ACACCCTGCC TCAATCAGTT TCCCCAGGAG CTCAATGTGG GGAAGATCAG TGCTGAGGTG 
ATGTGGAGCC TATTTGCCCA AGACATGAAG TACGCCATGG AGGAACATGA CAAACACCGG 
CTGTGTAAGA GCGCAGATTA CATGAACCTG CACTTCAAGG TGAAGTGGCT GTACAATGAG 
TACGTGGCCG AGCTGCCCAC CTTCAAGGAC CGTGTGCCTG AGTACCCTGC GTGGTTTGAG 
CCCTTCGTCA TCCAGTGGTT GGATGAGAAT GAGGAGGTGT CCCGGGACTT CCTGCATGGT 
GCCCTCGAGC GGGACAAGAA GGACGGGTTC CAGCAGACCT CAGAGCACGC CCTGTTCTCT 
TGCTCAGTGG TGGACGTCTT CTCCCAGCTT AACCAGAGTT TCGAGATCAT CAAGAAGCTG 
GAGTGTCCTG ACCCCCAGAT CGTGGGCCAT TACATGCGGC GTTTTGCCAA GACCATTAGC 
AATGTGCTTC TCCAGTATGC AGACATCGTT TCCAAGGACT TCGCCTCCTA CTGCTCCAAG 
GAGAAGGAGA AAGTGCCCTG CATCCTCATG AACAACACAC AGCAACTACG AGTGCAGCTG 
GAGAAGATGT TTGAGGCGAT GGGCGGGAAG GAGCTGGATG CCGAGGCCAG TGGCACCCTG 
AAGGAGCTGC AGGTGAAACT CAACAATGTC CTGGATGAAC TCAGCCATGT GTTTGCCACC 
AGCTTCCAGC CACACATTGA GGAATGCGTC AGACAGATGG GTGACATCCT GAGCCAGGTG 
AAGGGCACAG GCAATGTACC TGCCAGTGCC TGCAGCAGCG TGGCCCAGGA TGCAGACAAT 
GTGCTGCAGC CCATCATGGA TCTTCTGGAC AGCAACCTCA CCCTGTTTGC CAAAATCTGT 
GAGAAGACAG TTCTGAAGCG GGTGCTGAAG GAGCTCTGGA AGCTGGTGAT GAACACAATG 
GAGAAGACCA TCGTCCTGCC ACCGCTCACT GACCAGACGG GGACACAAAT GATCTTCAAT 
GCTGCCAAGG AGCTGGGCCA GCTGTCCAAA CTTAAGGATC ACATGGTGCG GGAAGAAGCC 
AAGAGCTTGA CCCCGAAGCA GTGTGCTGTT GTTGAACTGG CCCTGGACAC CATCAAGCAA 
TACTTCCACG CGGGGGGCGT TGGCCTCAAG AAGACCTTCC TGGAGAAGAG CCCGGACCTC 
CAGTCCCTGC GCTACGCCCT GTCGCTCTAC ACGCAAGCCA CCGACCTGCT CATCAAAACC 
TTCGTGCAGA CGCAGTCAGC GCAGGGCTCT GGTGTGGAGG ACCCTGTAGG TGAAGTGTCC 
GTCCACGTGG AGCTGTTCAC ACATCCAGGA ACTGGGGAAC AGAAGGTCAC AGTGAAGGTG 
GTGGCCGCCA ATGACCTCAA GTGGCAGACT TCTGGCATCT TCCGCCCGTT CATCGAGGTC 
AACATCGTTG GACCTCAGCT TAGCGACAAG AAACGCAAGT TCGCCACCAA ATCCAAAAAC 
AACAGCTGGG CGCCCAAGTA TAACGAGAGC TTCCAGTTCT CCCTGAGCGC CGACGCGGGC 
CCCGAGTGCT ACGAGCTGCA GGTGTGCGTG AAGGACTACT GCTTTGCGCG CGAAGACCGC 
ACGGTGGGGC TGGCGGTGCT GCAACTGCGA GAGCTGGCCC AGCGCGGGAG CGCCGCGTGC 
TGGCTTCCAC TCGGCCGCCG CATCCACATG GACGACACGG GGCTCACAGT GCTGCGCATC 
CTGTCGCAGC GCAGCAACGA TGAGGTGGCC AAGGAATTCG TCAAGCTCAA GTCGGACACG 
CGCTCAGCCG AGGAGGGCGG TGCCGCGCCT GCGCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168370.1
CDS	127..5205
Translation	MSLLCVGVKKAKFDGAQEKFNTYVTLKVQNVKSTTIAVRGSQPSWEQDFMFEINRLDLGLTVEVWNKGLIWDTMVGTVWIPLRTIRQSNEEGPGEWLTLDSQAIMADSEICGTKDPTFHRILLDAHFELPLDIPEEEARYWAKKLEQLNAMRDQDEVSHLQYSFQDQQDKPLPVPSSQCCNWNYFGWGEQNDDPDSAVDDRDSDYRSETSNSIPPPYYTTSQPNASVHQYSVRPPPLGSRESYSDSMHSYEEFSEPRALSPTGSSRYASSGELSQGSSQLSEDFDPDEHSLQGSELDDERDRDSYHSCHSSVSYHKDSPRWDQDDEDLEDLEDLEDEELPEEEEELEEEGEEELEEEDLEEEEEVPDDLASYTQQEDTTVAEPKEFKRISFPTAAPQKDDKVSAVPTEAPEVAKGIPKAATPEEKAAAERAQEAEPPKSEESFRSREEEEGQEGQDAMSRAKANWLRAFNKVRMQLQEARGEGDMSKSLWFKGGPGGGLIIIDSMPDIRKRKPIPLVSDLAMSLVQSRKAGITSALASSTLNNEELKNHVYKKTLQALIYPISCTTPHNFEVWTATTPTYCYECEGLLWGIARQGMRCTECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMVLKDRMKIRERNKPEIFELIQEIFAVTKSAHTQQMKAVKQSVLDGTSKWSAKISITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIYGNLNPVWEENFHFECHNSSDRIKVRVWDEDDDIKSRVKQRFKRESDDFLGQTIIEVRTLSGEMDVWYNLDKRTDKSAVSGAIRLHISVEIKGEEKVAPYHVQYTCLHENLFHFVTDVQNNGVVKIPDAKGDDAWKVYYDETAQEIVDEFAMRYGVESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSMYRNNFPASSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHELYGREYQTDPAKKGEVPPEEQGPSIKNLDFWSKLITLIVSIIEEDKNSYTPCLNQFPQELNVGKISAEVMWSLFAQDMKYAMEEHDKHRLCKSADYMNLHFKVKWLYNEYVAELPTFKDRVPEYPAWFEPFVIQWLDENEEVSRDFLHGALERDKKDGFQQTSEHALFSCSVVDVFSQLNQSFEIIKKLECPDPQIVGHYMRRFAKTISNVLLQYADIVSKDFASYCSKEKEKVPCILMNNTQQLRVQLEKMFEAMGGKELDAEASGTLKELQVKLNNVLDELSHVFATSFQPHIEECVRQMGDILSQVKGTGNVPASACSSVAQDADNVLQPIMDLLDSNLTLFAKICEKTVLKRVLKELWKLVMNTMEKTIVLPPLTDQTGTQMIFNAAKELGQLSKLKDHMVREEAKSLTPKQCAVVELALDTIKQYFHAGGVGLKKTFLEKSPDLQSLRYALSLYTQATDLLIKTFVQTQSAQGSGVEDPVGEVSVHVELFTHPGTGEQKVTVKVVAANDLKWQTSGIFRPFIEVNIVGPQLSDKKRKFATKSKNNSWAPKYNESFQFSLSADAGPECYELQVCVKDYCFAREDRTVGLAVLQLRELAQRGSAACWLPLGRRIHMDDTGLTVLRILSQRSNDEVAKEFVKLKSDTRSAEEGGAAPAP

Target ORF information:

RefSeq Version	XM_017312881.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus unc-13 homolog A (Unc13a), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312881.2

ORF Insert Sequence:

ATGTCTCTGC TGTGCGTGGG AGTCAAAAAA GCCAAGTTTG ACGGTGCCCA AGAGAAGTTC 
AACACATACG TGACGCTGAA GGTGCAGAAC GTGAAGAGCA CTACCATAGC TGTACGTGGC 
AGCCAGCCCA GCTGGGAGCA GGACTTCATG TTTGAGATCA ACCGCCTGGA TCTAGGCCTG 
ACCGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGTAC TGTGTGGATC 
CCGCTGCGGA CCATCCGCCA GTCCAATGAG GAGGGCCCAG GAGAGTGGCT CACACTGGAC 
TCTCAGGCCA TCATGGCGGA CAGTGAGATC TGTGGGACCA AGGACCCCAC CTTCCATCGC 
ATCCTCCTGG ACGCTCATTT CGAGCTGCCG TTGGACATCC CTGAGGAGGA GGCACGCTAC 
TGGGCCAAGA AGCTGGAGCA GCTGAATGCC ATGCGTGACC AGGATGAGGT GAGTCACCTC 
CAGTACTCCT TCCAGGACCA GCAGGACAAG CCGCTGCCAG TGCCCAGCAG CCAGTGCTGC 
AACTGGAATT ACTTTGGCTG GGGAGAACAG AATGACGACC CCGACAGTGC GGTGGATGAC 
CGGGACAGTG ACTATAGGAG TGAGACCAGC AACAGCATCC CACCGCCCTA CTATACGACT 
TCGCAGCCCA ACGCCTCTGT GCACCAGTAC TCCGTGCGAC CGCCCCCTCT GGGGTCCCGG 
GAGTCCTACA GTGACTCCAT GCACAGCTAT GAGGAGTTCT CTGAGCCGCG GGCGCTCAGC 
CCCACAGGCA GCAGCCGCTA TGCTTCTTCC GGGGAGCTGA GCCAGGGAAG CTCACAGCTG 
AGCGAGGACT TCGACCCTGA TGAGCACAGC CTGCAGGGCT CGGAGCTGGA TGACGAGAGG 
GACCGCGATT CTTATCACTC CTGTCACAGC TCCGTGAGTT ACCACAAGGA CTCACCCCGC 
TGGGACCAGG ACGATGAGGA CCTGGAGGAC CTGGAGGACC TGGAAGATGA GGAGCTGCCC 
GAGGAGGAGG AGGAGTTGGA GGAGGAGGGG GAGGAGGAAT TGGAGGAAGA GGATTTGGAG 
GAGGAGGAGG AGGTGCCTGA TGACCTGGCC AGCTACACCC AGCAGGAGGA CACCACTGTG 
GCTGAGCCCA AAGAGTTCAA GCGGATCAGC TTCCCAACGG CTGCGCCTCA GAAGGATGAC 
AAGGTTTCAG CTGTGCCTAC AGAGGCCCCC GAGGTGGCCA AAGGCATCCC CAAAGCAGCC 
ACACCCGAGG AGAAGGCAGC TGCAGAGCGT GCGCAGGAAG CCGAGCCCCC CAAGTCTGAG 
GAGAGTTTCA GATCTCGAGA GGAGGAAGAG GGCCAGGAAG GGCAGGATGC CATGTCCAGA 
GCCAAAGCCA ACTGGTTGCG AGCCTTCAAC AAGGTGCGCA TGCAGCTGCA GGAGGCCCGA 
GGAGAAGGGG ATATGTCTAA GTCTCTATGG TTCAAAGGCG GTCCTGGTGG TGGCCTTATC 
ATCATTGACA GCATGCCAGA CATCAGGAAA CGGAAGCCCA TTCCCCTCGT GAGCGACCTG 
GCTATGTCAT TGGTGCAGTC TCGGAAGGCA GGCATCACCT CGGCCTTGGC CTCCAGCACG 
TTGAACAATG AAGAGCTGAA AAATCACGTT TACAAGAAGA CCCTGCAAGC CTTAATCTAC 
CCCATCTCCT GCACCACGCC GCACAATTTC GAGGTGTGGA CGGCCACCAC GCCCACCTAC 
TGCTATGAGT GCGAGGGGCT GCTGTGGGGC ATCGCGCGGC AGGGCATGCG ATGCACCGAG 
TGCGGCGTTA AGTGCCACGA GAAGTGCCAG GACCTGCTCA ATGCGGATTG CCTGCAGCGG 
GCGGCTGAGA AGAGTTCCAA GCATGGCGCT GAAGACCGCA CGCAGAACAT CATCATGGTG 
CTGAAGGACC GCATGAAGAT CCGCGAGCGC AACAAGCCTG AGATCTTCGA GCTTATCCAA 
GAGATCTTCG CGGTCACCAA GAGCGCACAC ACACAGCAGA TGAAAGCTGT CAAGCAGAGT 
GTGCTGGACG GCACATCCAA GTGGTCTGCC AAAATTAGCA TCACAGTGGT CTGTGCCCAG 
GGCTTGCAGG CCAAGGACAA GACAGGATCC AGTGACCCTT ATGTCACCGT CCAGGTTGGG 
AAGACCAAGA AAAGGACAAA AACCATCTAC GGGAACCTCA ACCCAGTGTG GGAAGAGAAT 
TTTCATTTTG AATGTCACAA CTCCTCTGAC CGGATCAAGG TTCGTGTTTG GGATGAAGAT 
GACGACATAA AATCCCGTGT GAAACAAAGG TTTAAGAGGG AGTCTGATGA CTTCCTAGGG 
CAGACAATCA TCGAGGTGCG GACGCTTAGC GGCGAGATGG ATGTGTGGTA CAATCTGGAC 
AAGAGAACGG ACAAGTCGGC GGTGTCGGGG GCCATCCGGC TTCACATCAG TGTGGAGATC 
AAAGGGGAGG AGAAGGTGGC GCCCTACCAT GTCCAGTACA CCTGTCTGCA TGAGAACCTG 
TTCCACTTTG TGACGGACGT GCAGAACAAT GGTGTGGTGA AGATTCCCGA TGCCAAGGGT 
GACGACGCCT GGAAGGTTTA CTACGATGAG ACGGCCCAGG AGATCGTGGA TGAGTTTGCC 
ATGCGCTATG GTGTTGAGTC CATCTACCAA GCCATGACCC ACTTTGCCTG CCTCTCCTCC 
AAGTATATGT GCCCTGGGGT ACCCGCTGTC ATGAGCACCC TGCTTGCCAA CATCAACGCC 
TACTACGCAC ACACCACCGC CTCCACCAAC GTGTCTGCCT CTGACCGCTT CGCTGCCTCT 
AATTTCGGGA AAGAGCGCTT TGTGAAACTT CTGGACCAGC TACACAATTC TCTGCGGATC 
GACCTGTCCA TGTACCGGAA CAACTTCCCA GCCAGCAGCC CTGAGCGGCT GCAGGATCTC 
AAGTCCACGG TGGACCTGCT CACCAGCATC ACCTTCTTCC GGATGAAGGT TCAGGAACTG 
CAGAGCCCGC CGCGTGCCAG CCAGGTAGTG AAAGACTGTG TGAAGGCCTG CCTCAACTCC 
ACCTATGAGT ACATCTTCAA CAACTGCCAT GAGCTCTATG GCCGGGAGTA CCAAACGGAT 
CCGGCCAAGA AGGGGGAGGT TCCCCCAGAG GAGCAAGGCC CTAGCATCAA GAACCTGGAT 
TTCTGGTCCA AGCTGATCAC CCTCATCGTG TCTATCATCG AGGAGGATAA GAATTCTTAC 
ACACCCTGCC TCAATCAGTT TCCCCAGGAG CTCAATGTGG GGAAGATCAG TGCTGAGGTG 
ATGTGGAGCC TATTTGCCCA AGACATGAAG TACGCCATGG AGGAACATGA CAAACACCGG 
CTGTGTAAGA GCGCAGATTA CATGAACCTG CACTTCAAGG TGAAGTGGCT GTACAATGAG 
TACGTGGCCG AGCTGCCCAC CTTCAAGGAC CGTGTGCCTG AGTACCCTGC GTGGTTTGAG 
CCCTTCGTCA TCCAGTGGTT GGATGAGAAT GAGGAGGTGT CCCGGGACTT CCTGCATGGT 
GCCCTCGAGC GGGACAAGAA GGACGGGTTC CAGCAGACCT CAGAGCACGC CCTGTTCTCT 
TGCTCAGTGG TGGACGTCTT CTCCCAGCTT AACCAGAGTT TCGAGATCAT CAAGAAGCTG 
GAGTGTCCTG ACCCCCAGAT CGTGGGCCAT TACATGCGGC GTTTTGCCAA GACCATTAGC 
AATGTGCTTC TCCAGTATGC AGACATCGTT TCCAAGGACT TCGCCTCCTA CTGCTCCAAG 
GAGAAGGAGA AAGTGCCCTG CATCCTCATG AACAACACAC AGCAACTACG AGTGCAGCTG 
GAGAAGATGT TTGAGGCGAT GGGCGGGAAG GAGCTGGATG CCGAGGCCAG TGGCACCCTG 
AAGGAGCTGC AGGTGAAACT CAACAATGTC CTGGATGAAC TCAGCCATGT GTTTGCCACC 
AGCTTCCAGC CACACATTGA GGAATGCGTC AGACAGATGG GTGACATCCT GAGCCAGGTG 
AAGGGCACAG GCAATGTACC TGCCAGTGCC TGCAGCAGCG TGGCCCAGGA TGCAGACAAT 
GTGCTGCAGC CCATCATGGA TCTTCTGGAC AGCAACCTCA CCCTGTTTGC CAAAATCTGT 
GAGAAGACAG TTCTGAAGCG GGTGCTGAAG GAGCTCTGGA AGCTGGTGAT GAACACAATG 
GAGAAGACCA TCGTCCTGCC ACCGCTCACT GACCAGACGG GGACACAAAT GATCTTCAAT 
GCTGCCAAGG AGCTGGGCCA GCTGTCCAAA CTTAAGGATC ACATGGTGCG GGAAGAAGCC 
AAGAGCTTGA CCCCGAAGCA GTGTGCTGTT GTTGAACTGG CCCTGGACAC CATCAAGCAA 
TACTTCCACG CGGGGGGCGT TGGCCTCAAG AAGACCTTCC TGGAGAAGAG CCCGGACCTC 
CAGTCCCTGC GCTACGCCCT GTCGCTCTAC ACGCAAGCCA CCGACCTGCT CATCAAAACC 
TTCGTGCAGA CGCAGTCAGC GCAGGGCTCT GGTGTGGAGG ACCCTGTAGG TGAAGTGTCC 
GTCCACGTGG AGCTGTTCAC ACATCCAGGA ACTGGGGAAC AGAAGGTCAC AGTGAAGGTG 
GTGGCCGCCA ATGACCTCAA GTGGCAGACT TCTGGCATCT TCCGCCCGTT CATCGAGGTC 
AACATCGTTG GACCTCAGCT TAGCGACAAG AAACGCAAGT TCGCCACCAA ATCCAAAAAC 
AACAGCTGGG CGCCCAAGTA TAACGAGAGC TTCCAGTTCT CCCTGAGCGC CGACGCGGGC 
CCCGAGTGCT ACGAGCTGCA GGTGTGCGTG AAGGACTACT GCTTTGCGCG CGAAGACCGC 
ACGGTGGGGC TGGCGGTGCT GCAACTGCGA GAGCTGGCCC AGCGCGGGAG CGCCGCGTGC 
TGGCTTCCAC TCGGCCGCCG CATCCACATG GACGACACGG GGCTCACAGT GCTGCGCATC 
CTGTCGCAGC GCAGCAACGA TGAGGTGGCC AAGGAATTCG TCAAGCTCAA GTCGGACACG 
CGCTCAGCCG AGGAGGGCGG TGCCGCGCCT GCGCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Unc13a ( XM_017312882.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168371.1 Mus musculus unc-13 homolog A (Unc13a), transcript variant X13, mRNA.

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CloneID	OMu82532
Clone ID Related Accession (Same CDS sequence)	XM_017312882.1 , XM_017312882.2
Accession Version	XM_017312882.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein unc-13 homolog A isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017312882.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTGC TGTGCGTGGG AGTCAAAAAA GCCAAGTTTG ACGGTGCCCA AGAGAAGTTC 
AACACATACG TGACGCTGAA GGTGCAGAAC GTGAAGAGCA CTACCATAGC TGTACGTGGC 
AGCCAGCCCA GCTGGGAGCA GGACTTCATG TTTGAGATCA ACCGCCTGGA TCTAGGCCTG 
ACCGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGTAC TGTGTGGATC 
CCGCTGCGGA CCATCCGCCA GTCCAATGAG GAGGGCCCAG GAGAGTGGCT CACACTGGAC 
TCTCAGGCCA TCATGGCGGA CAGTGAGATC TGTGGGACCA AGGACCCCAC CTTCCATCGC 
ATCCTCCTGG ACGCTCATTT CGAGCTGCCG TTGGACATCC CTGAGGAGGA GGCACGCTAC 
TGGGCCAAGA AGCTGGAGCA GCTGAATGCC ATGCGTGACC AGGATGAGTA CTCCTTCCAG 
GACCAGCAGG ACAAGCCGCT GCCAGTGCCC AGCAGCCAGT GCTGCAACTG GAATTACTTT 
GGCTGGGGAG AACAGAATGA CGACCCCGAC AGTGCGGTGG ATGACCGGGA CAGTGACTAT 
AGGAGTGAGA CCAGCAACAG CATCCCACCG CCCTACTATA CGACTTCGCA GCCCAACGCC 
TCTGTGCACC AGTACTCCGT GCGACCGCCC CCTCTGGGGT CCCGGGAGTC CTACAGTGAC 
TCCATGCACA GCTATGAGGA GTTCTCTGAG CCGCGGGCGC TCAGCCCCAC AGGCAGCAGC 
CGCTATGCTT CTTCCGGGGA GCTGAGCCAG GGAAGCTCAC AGCTGAGCGA GGACTTCGAC 
CCTGATGAGC ACAGCCTGCA GGGCTCGGAG CTGGATGACG AGAGGGACCG CGATTCTTAT 
CACTCCTGTC ACAGCTCCGT GAGTTACCAC AAGGACTCAC CCCGCTGGGA CCAGGACGAT 
GAGGACCTGG AGGACCTGGA GGACCTGGAA GATGAGGAGC TGCCCGAGGA GGAGGAGGAG 
TTGGAGGAGG AGGGGGAGGA GGAATTGGAG GAAGAGGATT TGGAGGAGGA GGAGGAGGTG 
CCTGATGACC TGGCCAGCTA CACCCAGCAG GAGGACACCA CTGTGGCTGA GCCCAAAGAG 
TTCAAGCGGA TCAGCTTCCC AACGGCTGCG CCTCAGAAGG ATGACAAGGT TTCAGCTGTG 
CCTACAGAGG CCCCCGAGGT GGCCAAAGGC ATCCCCAAAG CAGCCACACC CGAGGAGAAG 
GCAGCTGCAG AGCGTGCGCA GGAAGCCGAG CCCCCCAAGT CTGAGGAGAG TTTCAGATCT 
CGAGAGGAGG AAGAGGGCCA GGAAGGGCAG GATGCCATGT CCAGAGCCAA AGCCAACTGG 
TTGCGAGCCT TCAACAAGGT GCGCATGCAG CTGCAGGAGG CCCGAGGAGA AGGGGATATG 
TCTAAGTCTC TATGGTTCAA AGGCGGTCCT GGTGGTGGCC TTATCATCAT TGACAGCATG 
CCAGACATCA GGAAACGGAA GCCCATTCCC CTCGTGAGCG ACCTGGCTAT GTCATTGGTG 
CAGTCTCGGA AGGCAGGCAT CACCTCGGCC TTGGCCTCCA GCACGTTGAA CAATGAAGAG 
CTGAAAAATC ACGTTTACAA GAAGACCCTG CAAGCCTTAA TCTACCCCAT CTCCTGCACC 
ACGCCGCACA ATTTCGAGGT GTGGACGGCC ACCACGCCCA CCTACTGCTA TGAGTGCGAG 
GGGCTGCTGT GGGGCATCGC GCGGCAGGGC ATGCGATGCA CCGAGTGCGG CGTTAAGTGC 
CACGAGAAGT GCCAGGACCT GCTCAATGCG GATTGCCTGC AGCGGGCGGC TGAGAAGAGT 
TCCAAGCATG GCGCTGAAGA CCGCACGCAG AACATCATCA TGGTGCTGAA GGACCGCATG 
AAGATCCGCG AGCGCAACAA GCCTGAGATC TTCGAGCTTA TCCAAGAGAT CTTCGCGGTC 
ACCAAGAGCG CACACACACA GCAGATGAAA GCTGTCAAGC AGAGTGTGCT GGACGGCACA 
TCCAAGTGGT CTGCCAAAAT TAGCATCACA GTGGTCTGTG CCCAGGGCTT GCAGGCCAAG 
GACAAGACAG GATCCAGTGA CCCTTATGTC ACCGTCCAGG TTGGGAAGAC CAAGAAAAGG 
ACAAAAACCA TCTACGGGAA CCTCAACCCA GTGTGGGAAG AGAATTTTCA TTTTGAATGT 
CACAACTCCT CTGACCGGAT CAAGGTTCGT GTTTGGGATG AAGATGACGA CATAAAATCC 
CGTGTGAAAC AAAGGTTTAA GAGGGAGTCT GATGACTTCC TAGGGCAGAC AATCATCGAG 
GTGCGGACGC TTAGCGGCGA GATGGATGTG TGGTACAATC TGGACAAGAG AACGGACAAG 
TCGGCGGTGT CGGGGGCCAT CCGGCTTCAC ATCAGTGTGG AGATCAAAGG GGAGGAGAAG 
GTGGCGCCCT ACCATGTCCA GTACACCTGT CTGCATGAGA ACCTGTTCCA CTTTGTGACG 
GACGTGCAGA ACAATGGTGT GGTGAAGATT CCCGATGCCA AGGGTGACGA CGCCTGGAAG 
GTTTACTACG ATGAGACGGC CCAGGAGATC GTGGATGAGT TTGCCATGCG CTATGGTGTT 
GAGTCCATCT ACCAAGCCAT GACCCACTTT GCCTGCCTCT CCTCCAAGTA TATGTGCCCT 
GGGGTACCCG CTGTCATGAG CACCCTGCTT GCCAACATCA ACGCCTACTA CGCACACACC 
ACCGCCTCCA CCAACGTGTC TGCCTCTGAC CGCTTCGCTG CCTCTAATTT CGGGAAAGAG 
CGCTTTGTGA AACTTCTGGA CCAGCTACAC AATTCTCTGC GGATCGACCT GTCCATGTAC 
CGGAACAACT TCCCAGCCAG CAGCCCTGAG CGGCTGCAGG ATCTCAAGTC CACGGTGGAC 
CTGCTCACCA GCATCACCTT CTTCCGGATG AAGGTTCAGG AACTGCAGAG CCCGCCGCGT 
GCCAGCCAGG TAGTGAAAGA CTGTGTGAAG GCCTGCCTCA ACTCCACCTA TGAGTACATC 
TTCAACAACT GCCATGAGCT CTATGGCCGG GAGTACCAAA CGGATCCGGC CAAGAAGGGG 
GAGGTTCCCC CAGAGGAGCA AGGCCCTAGC ATCAAGAACC TGGATTTCTG GTCCAAGCTG 
ATCACCCTCA TCGTGTCTAT CATCGAGGAG GATAAGAATT CTTACACACC CTGCCTCAAT 
CAGTTTCCCC AGGAGCTCAA TGTGGGGAAG ATCAGTGCTG AGGTGATGTG GAGCCTATTT 
GCCCAAGACA TGAAGTACGC CATGGAGGAA CATGACAAAC ACCGGCTGTG TAAGAGCGCA 
GATTACATGA ACCTGCACTT CAAGGTGAAG TGGCTGTACA ATGAGTACGT GGCCGAGCTG 
CCCACCTTCA AGGACCGTGT GCCTGAGTAC CCTGCGTGGT TTGAGCCCTT CGTCATCCAG 
TGGTTGGATG AGAATGAGGA GGTGTCCCGG GACTTCCTGC ATGGTGCCCT CGAGCGGGAC 
AAGAAGGACG GGTTCCAGCA GACCTCAGAG CACGCCCTGT TCTCTTGCTC AGTGGTGGAC 
GTCTTCTCCC AGCTTAACCA GAGTTTCGAG ATCATCAAGA AGCTGGAGTG TCCTGACCCC 
CAGATCGTGG GCCATTACAT GCGGCGTTTT GCCAAGACCA TTAGCAATGT GCTTCTCCAG 
TATGCAGACA TCGTTTCCAA GGACTTCGCC TCCTACTGCT CCAAGGAGAA GGAGAAAGTG 
CCCTGCATCC TCATGAACAA CACACAGCAA CTACGAGTGC AGCTGGAGAA GATGTTTGAG 
GCGATGGGCG GGAAGGAGCT GGATGCCGAG GCCAGTGGCA CCCTGAAGGA GCTGCAGGTG 
AAACTCAACA ATGTCCTGGA TGAACTCAGC CATGTGTTTG CCACCAGCTT CCAGCCACAC 
ATTGAGGAAT GCGTCAGACA GATGGGTGAC ATCCTGAGCC AGGTGAAGGG CACAGGCAAT 
GTACCTGCCA GTGCCTGCAG CAGCGTGGCC CAGGATGCAG ACAATGTGCT GCAGCCCATC 
ATGGATCTTC TGGACAGCAA CCTCACCCTG TTTGCCAAAA TCTGTGAGAA GACAGTTCTG 
AAGCGGGTGC TGAAGGAGCT CTGGAAGCTG GTGATGAACA CAATGGAGAA GACCATCGTC 
CTGCCACCGC TCACTGACCA GACGGGGACA CAAATGATCT TCAATGCTGC CAAGGAGCTG 
GGCCAGCTGT CCAAACTTAA GGATCACATG GTGCGGGAAG AAGCCAAGAG CTTGACCCCG 
AAGCAGTGTG CTGTTGTTGA ACTGGCCCTG GACACCATCA AGCAATACTT CCACGCGGGG 
GGCGTTGGCC TCAAGAAGAC CTTCCTGGAG AAGAGCCCGG ACCTCCAGTC CCTGCGCTAC 
GCCCTGTCGC TCTACACGCA AGCCACCGAC CTGCTCATCA AAACCTTCGT GCAGACGCAG 
TCAGCGCAGG TCCATGGTGG AAAAGGGACT AGGTTTACCC TTAGTGAAGA CGTTTGTCCT 
GAGATGGGCT CTGGTGTGGA GGACCCTGTA GGTGAAGTGT CCGTCCACGT GGAGCTGTTC 
ACACATCCAG GAACTGGGGA ACAGAAGGTC ACAGTGAAGG TGGTGGCCGC CAATGACCTC 
AAGTGGCAGA CTTCTGGCAT CTTCCGCCCG TTCATCGAGG TCAACATCGT TGGACCTCAG 
CTTAGCGACA AGAAACGCAA GTTCGCCACC AAATCCAAAA ACAACAGCTG GGCGCCCAAG 
TATAACGAGA GCTTCCAGTT CTCCCTGAGC GCCGACGCGG GCCCCGAGTG CTACGAGCTG 
CAGGTGTGCG TGAAGGACTA CTGCTTTGCG CGCGAAGACC GCACGGTGGG GCTGGCGGTG 
CTGCAACTGC GAGAGCTGGC CCAGCGCGGG AGCGCCGCGT GCTGGCTTCC ACTCGGCCGC 
CGCATCCACA TGGACGACAC GGGGCTCACA GTGCTGCGCA TCCTGTCGCA GCGCAGCAAC 
GATGAGGTGG CCAAGGAATT CGTCAAGCTC AAGTCGGACA CGCGCTCAGC CGAGGAGGGC 
GGTGCCGCGC CTGCGCCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168371.1
CDS	127..5247
Translation	MSLLCVGVKKAKFDGAQEKFNTYVTLKVQNVKSTTIAVRGSQPSWEQDFMFEINRLDLGLTVEVWNKGLIWDTMVGTVWIPLRTIRQSNEEGPGEWLTLDSQAIMADSEICGTKDPTFHRILLDAHFELPLDIPEEEARYWAKKLEQLNAMRDQDEYSFQDQQDKPLPVPSSQCCNWNYFGWGEQNDDPDSAVDDRDSDYRSETSNSIPPPYYTTSQPNASVHQYSVRPPPLGSRESYSDSMHSYEEFSEPRALSPTGSSRYASSGELSQGSSQLSEDFDPDEHSLQGSELDDERDRDSYHSCHSSVSYHKDSPRWDQDDEDLEDLEDLEDEELPEEEEELEEEGEEELEEEDLEEEEEVPDDLASYTQQEDTTVAEPKEFKRISFPTAAPQKDDKVSAVPTEAPEVAKGIPKAATPEEKAAAERAQEAEPPKSEESFRSREEEEGQEGQDAMSRAKANWLRAFNKVRMQLQEARGEGDMSKSLWFKGGPGGGLIIIDSMPDIRKRKPIPLVSDLAMSLVQSRKAGITSALASSTLNNEELKNHVYKKTLQALIYPISCTTPHNFEVWTATTPTYCYECEGLLWGIARQGMRCTECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMVLKDRMKIRERNKPEIFELIQEIFAVTKSAHTQQMKAVKQSVLDGTSKWSAKISITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIYGNLNPVWEENFHFECHNSSDRIKVRVWDEDDDIKSRVKQRFKRESDDFLGQTIIEVRTLSGEMDVWYNLDKRTDKSAVSGAIRLHISVEIKGEEKVAPYHVQYTCLHENLFHFVTDVQNNGVVKIPDAKGDDAWKVYYDETAQEIVDEFAMRYGVESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSMYRNNFPASSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHELYGREYQTDPAKKGEVPPEEQGPSIKNLDFWSKLITLIVSIIEEDKNSYTPCLNQFPQELNVGKISAEVMWSLFAQDMKYAMEEHDKHRLCKSADYMNLHFKVKWLYNEYVAELPTFKDRVPEYPAWFEPFVIQWLDENEEVSRDFLHGALERDKKDGFQQTSEHALFSCSVVDVFSQLNQSFEIIKKLECPDPQIVGHYMRRFAKTISNVLLQYADIVSKDFASYCSKEKEKVPCILMNNTQQLRVQLEKMFEAMGGKELDAEASGTLKELQVKLNNVLDELSHVFATSFQPHIEECVRQMGDILSQVKGTGNVPASACSSVAQDADNVLQPIMDLLDSNLTLFAKICEKTVLKRVLKELWKLVMNTMEKTIVLPPLTDQTGTQMIFNAAKELGQLSKLKDHMVREEAKSLTPKQCAVVELALDTIKQYFHAGGVGLKKTFLEKSPDLQSLRYALSLYTQATDLLIKTFVQTQSAQVHGGKGTRFTLSEDVCPEMGSGVEDPVGEVSVHVELFTHPGTGEQKVTVKVVAANDLKWQTSGIFRPFIEVNIVGPQLSDKKRKFATKSKNNSWAPKYNESFQFSLSADAGPECYELQVCVKDYCFAREDRTVGLAVLQLRELAQRGSAACWLPLGRRIHMDDTGLTVLRILSQRSNDEVAKEFVKLKSDTRSAEEGGAAPAP

Target ORF information:

RefSeq Version	XM_017312882.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus unc-13 homolog A (Unc13a), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312882.2

ORF Insert Sequence:

ATGTCTCTGC TGTGCGTGGG AGTCAAAAAA GCCAAGTTTG ACGGTGCCCA AGAGAAGTTC 
AACACATACG TGACGCTGAA GGTGCAGAAC GTGAAGAGCA CTACCATAGC TGTACGTGGC 
AGCCAGCCCA GCTGGGAGCA GGACTTCATG TTTGAGATCA ACCGCCTGGA TCTAGGCCTG 
ACCGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGTAC TGTGTGGATC 
CCGCTGCGGA CCATCCGCCA GTCCAATGAG GAGGGCCCAG GAGAGTGGCT CACACTGGAC 
TCTCAGGCCA TCATGGCGGA CAGTGAGATC TGTGGGACCA AGGACCCCAC CTTCCATCGC 
ATCCTCCTGG ACGCTCATTT CGAGCTGCCG TTGGACATCC CTGAGGAGGA GGCACGCTAC 
TGGGCCAAGA AGCTGGAGCA GCTGAATGCC ATGCGTGACC AGGATGAGTA CTCCTTCCAG 
GACCAGCAGG ACAAGCCGCT GCCAGTGCCC AGCAGCCAGT GCTGCAACTG GAATTACTTT 
GGCTGGGGAG AACAGAATGA CGACCCCGAC AGTGCGGTGG ATGACCGGGA CAGTGACTAT 
AGGAGTGAGA CCAGCAACAG CATCCCACCG CCCTACTATA CGACTTCGCA GCCCAACGCC 
TCTGTGCACC AGTACTCCGT GCGACCGCCC CCTCTGGGGT CCCGGGAGTC CTACAGTGAC 
TCCATGCACA GCTATGAGGA GTTCTCTGAG CCGCGGGCGC TCAGCCCCAC AGGCAGCAGC 
CGCTATGCTT CTTCCGGGGA GCTGAGCCAG GGAAGCTCAC AGCTGAGCGA GGACTTCGAC 
CCTGATGAGC ACAGCCTGCA GGGCTCGGAG CTGGATGACG AGAGGGACCG CGATTCTTAT 
CACTCCTGTC ACAGCTCCGT GAGTTACCAC AAGGACTCAC CCCGCTGGGA CCAGGACGAT 
GAGGACCTGG AGGACCTGGA GGACCTGGAA GATGAGGAGC TGCCCGAGGA GGAGGAGGAG 
TTGGAGGAGG AGGGGGAGGA GGAATTGGAG GAAGAGGATT TGGAGGAGGA GGAGGAGGTG 
CCTGATGACC TGGCCAGCTA CACCCAGCAG GAGGACACCA CTGTGGCTGA GCCCAAAGAG 
TTCAAGCGGA TCAGCTTCCC AACGGCTGCG CCTCAGAAGG ATGACAAGGT TTCAGCTGTG 
CCTACAGAGG CCCCCGAGGT GGCCAAAGGC ATCCCCAAAG CAGCCACACC CGAGGAGAAG 
GCAGCTGCAG AGCGTGCGCA GGAAGCCGAG CCCCCCAAGT CTGAGGAGAG TTTCAGATCT 
CGAGAGGAGG AAGAGGGCCA GGAAGGGCAG GATGCCATGT CCAGAGCCAA AGCCAACTGG 
TTGCGAGCCT TCAACAAGGT GCGCATGCAG CTGCAGGAGG CCCGAGGAGA AGGGGATATG 
TCTAAGTCTC TATGGTTCAA AGGCGGTCCT GGTGGTGGCC TTATCATCAT TGACAGCATG 
CCAGACATCA GGAAACGGAA GCCCATTCCC CTCGTGAGCG ACCTGGCTAT GTCATTGGTG 
CAGTCTCGGA AGGCAGGCAT CACCTCGGCC TTGGCCTCCA GCACGTTGAA CAATGAAGAG 
CTGAAAAATC ACGTTTACAA GAAGACCCTG CAAGCCTTAA TCTACCCCAT CTCCTGCACC 
ACGCCGCACA ATTTCGAGGT GTGGACGGCC ACCACGCCCA CCTACTGCTA TGAGTGCGAG 
GGGCTGCTGT GGGGCATCGC GCGGCAGGGC ATGCGATGCA CCGAGTGCGG CGTTAAGTGC 
CACGAGAAGT GCCAGGACCT GCTCAATGCG GATTGCCTGC AGCGGGCGGC TGAGAAGAGT 
TCCAAGCATG GCGCTGAAGA CCGCACGCAG AACATCATCA TGGTGCTGAA GGACCGCATG 
AAGATCCGCG AGCGCAACAA GCCTGAGATC TTCGAGCTTA TCCAAGAGAT CTTCGCGGTC 
ACCAAGAGCG CACACACACA GCAGATGAAA GCTGTCAAGC AGAGTGTGCT GGACGGCACA 
TCCAAGTGGT CTGCCAAAAT TAGCATCACA GTGGTCTGTG CCCAGGGCTT GCAGGCCAAG 
GACAAGACAG GATCCAGTGA CCCTTATGTC ACCGTCCAGG TTGGGAAGAC CAAGAAAAGG 
ACAAAAACCA TCTACGGGAA CCTCAACCCA GTGTGGGAAG AGAATTTTCA TTTTGAATGT 
CACAACTCCT CTGACCGGAT CAAGGTTCGT GTTTGGGATG AAGATGACGA CATAAAATCC 
CGTGTGAAAC AAAGGTTTAA GAGGGAGTCT GATGACTTCC TAGGGCAGAC AATCATCGAG 
GTGCGGACGC TTAGCGGCGA GATGGATGTG TGGTACAATC TGGACAAGAG AACGGACAAG 
TCGGCGGTGT CGGGGGCCAT CCGGCTTCAC ATCAGTGTGG AGATCAAAGG GGAGGAGAAG 
GTGGCGCCCT ACCATGTCCA GTACACCTGT CTGCATGAGA ACCTGTTCCA CTTTGTGACG 
GACGTGCAGA ACAATGGTGT GGTGAAGATT CCCGATGCCA AGGGTGACGA CGCCTGGAAG 
GTTTACTACG ATGAGACGGC CCAGGAGATC GTGGATGAGT TTGCCATGCG CTATGGTGTT 
GAGTCCATCT ACCAAGCCAT GACCCACTTT GCCTGCCTCT CCTCCAAGTA TATGTGCCCT 
GGGGTACCCG CTGTCATGAG CACCCTGCTT GCCAACATCA ACGCCTACTA CGCACACACC 
ACCGCCTCCA CCAACGTGTC TGCCTCTGAC CGCTTCGCTG CCTCTAATTT CGGGAAAGAG 
CGCTTTGTGA AACTTCTGGA CCAGCTACAC AATTCTCTGC GGATCGACCT GTCCATGTAC 
CGGAACAACT TCCCAGCCAG CAGCCCTGAG CGGCTGCAGG ATCTCAAGTC CACGGTGGAC 
CTGCTCACCA GCATCACCTT CTTCCGGATG AAGGTTCAGG AACTGCAGAG CCCGCCGCGT 
GCCAGCCAGG TAGTGAAAGA CTGTGTGAAG GCCTGCCTCA ACTCCACCTA TGAGTACATC 
TTCAACAACT GCCATGAGCT CTATGGCCGG GAGTACCAAA CGGATCCGGC CAAGAAGGGG 
GAGGTTCCCC CAGAGGAGCA AGGCCCTAGC ATCAAGAACC TGGATTTCTG GTCCAAGCTG 
ATCACCCTCA TCGTGTCTAT CATCGAGGAG GATAAGAATT CTTACACACC CTGCCTCAAT 
CAGTTTCCCC AGGAGCTCAA TGTGGGGAAG ATCAGTGCTG AGGTGATGTG GAGCCTATTT 
GCCCAAGACA TGAAGTACGC CATGGAGGAA CATGACAAAC ACCGGCTGTG TAAGAGCGCA 
GATTACATGA ACCTGCACTT CAAGGTGAAG TGGCTGTACA ATGAGTACGT GGCCGAGCTG 
CCCACCTTCA AGGACCGTGT GCCTGAGTAC CCTGCGTGGT TTGAGCCCTT CGTCATCCAG 
TGGTTGGATG AGAATGAGGA GGTGTCCCGG GACTTCCTGC ATGGTGCCCT CGAGCGGGAC 
AAGAAGGACG GGTTCCAGCA GACCTCAGAG CACGCCCTGT TCTCTTGCTC AGTGGTGGAC 
GTCTTCTCCC AGCTTAACCA GAGTTTCGAG ATCATCAAGA AGCTGGAGTG TCCTGACCCC 
CAGATCGTGG GCCATTACAT GCGGCGTTTT GCCAAGACCA TTAGCAATGT GCTTCTCCAG 
TATGCAGACA TCGTTTCCAA GGACTTCGCC TCCTACTGCT CCAAGGAGAA GGAGAAAGTG 
CCCTGCATCC TCATGAACAA CACACAGCAA CTACGAGTGC AGCTGGAGAA GATGTTTGAG 
GCGATGGGCG GGAAGGAGCT GGATGCCGAG GCCAGTGGCA CCCTGAAGGA GCTGCAGGTG 
AAACTCAACA ATGTCCTGGA TGAACTCAGC CATGTGTTTG CCACCAGCTT CCAGCCACAC 
ATTGAGGAAT GCGTCAGACA GATGGGTGAC ATCCTGAGCC AGGTGAAGGG CACAGGCAAT 
GTACCTGCCA GTGCCTGCAG CAGCGTGGCC CAGGATGCAG ACAATGTGCT GCAGCCCATC 
ATGGATCTTC TGGACAGCAA CCTCACCCTG TTTGCCAAAA TCTGTGAGAA GACAGTTCTG 
AAGCGGGTGC TGAAGGAGCT CTGGAAGCTG GTGATGAACA CAATGGAGAA GACCATCGTC 
CTGCCACCGC TCACTGACCA GACGGGGACA CAAATGATCT TCAATGCTGC CAAGGAGCTG 
GGCCAGCTGT CCAAACTTAA GGATCACATG GTGCGGGAAG AAGCCAAGAG CTTGACCCCG 
AAGCAGTGTG CTGTTGTTGA ACTGGCCCTG GACACCATCA AGCAATACTT CCACGCGGGG 
GGCGTTGGCC TCAAGAAGAC CTTCCTGGAG AAGAGCCCGG ACCTCCAGTC CCTGCGCTAC 
GCCCTGTCGC TCTACACGCA AGCCACCGAC CTGCTCATCA AAACCTTCGT GCAGACGCAG 
TCAGCGCAGG TCCATGGTGG AAAAGGGACT AGGTTTACCC TTAGTGAAGA CGTTTGTCCT 
GAGATGGGCT CTGGTGTGGA GGACCCTGTA GGTGAAGTGT CCGTCCACGT GGAGCTGTTC 
ACACATCCAG GAACTGGGGA ACAGAAGGTC ACAGTGAAGG TGGTGGCCGC CAATGACCTC 
AAGTGGCAGA CTTCTGGCAT CTTCCGCCCG TTCATCGAGG TCAACATCGT TGGACCTCAG 
CTTAGCGACA AGAAACGCAA GTTCGCCACC AAATCCAAAA ACAACAGCTG GGCGCCCAAG 
TATAACGAGA GCTTCCAGTT CTCCCTGAGC GCCGACGCGG GCCCCGAGTG CTACGAGCTG 
CAGGTGTGCG TGAAGGACTA CTGCTTTGCG CGCGAAGACC GCACGGTGGG GCTGGCGGTG 
CTGCAACTGC GAGAGCTGGC CCAGCGCGGG AGCGCCGCGT GCTGGCTTCC ACTCGGCCGC 
CGCATCCACA TGGACGACAC GGGGCTCACA GTGCTGCGCA TCCTGTCGCA GCGCAGCAAC 
GATGAGGTGG CCAAGGAATT CGTCAAGCTC AAGTCGGACA CGCGCTCAGC CGAGGAGGGC 
GGTGCCGCGC CTGCGCCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Unc13a ( XM_017312883.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168372.1 Mus musculus unc-13 homolog A (Unc13a), transcript variant X14, mRNA.

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CloneID	OMu82533
Clone ID Related Accession (Same CDS sequence)	XM_017312883.2 , XM_017312883.1
Accession Version	XM_017312883.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5064bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein unc-13 homolog A isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017312883.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTGC TGTGCGTGGG AGTCAAAAAA GCCAAGTTTG ACGGTGCCCA AGAGAAGTTC 
AACACATACG TGACGCTGAA GGTGCAGAAC GTGAAGAGCA CTACCATAGC TGTACGTGGC 
AGCCAGCCCA GCTGGGAGCA GGACTTCATG TTTGAGATCA ACCGCCTGGA TCTAGGCCTG 
ACCGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGTAC TGTGTGGATC 
CCGCTGCGGA CCATCCGCCA GTCCAATGAG GAGGGCCCAG GAGAGTGGCT CACACTGGAC 
TCTCAGGCCA TCATGGCGGA CAGTGAGATC TGTGGGACCA AGGACCCCAC CTTCCATCGC 
ATCCTCCTGG ACGCTCATTT CGAGCTGCCG TTGGACATCC CTGAGGAGGA GGCACGCTAC 
TGGGCCAAGA AGCTGGAGCA GCTGAATGCC ATGCGTGACC AGGATGAGTA CTCCTTCCAG 
GACCAGCAGG ACAAGCCGCT GCCAGTGCCC AGCAGCCAGT GCTGCAACTG GAATTACTTT 
GGCTGGGGAG AACAGAATGA CGACCCCGAC AGTGCGGTGG ATGACCGGGA CAGTGACTAT 
AGGAGTGAGA CCAGCAACAG CATCCCACCG CCCTACTATA CGACTTCGCA GCCCAACGCC 
TCTGTGCACC AGTACTCCGT GCGACCGCCC CCTCTGGGGT CCCGGGAGTC CTACAGTGAC 
TCCATGCACA GCTATGAGGA GTTCTCTGAG CCGCGGGCGC TCAGCCCCAC AGGCAGCAGC 
CGCTATGCTT CTTCCGGGGA GCTGAGCCAG GGAAGCTCAC AGCTGAGCGA GGACTTCGAC 
CCTGATGAGC ACAGCCTGCA GGGCTCGGAG CTGGATGACG AGAGGGACCG CGATTCTTAT 
CACTCCTGTC ACAGCTCCGT GAGTTACCAC AAGGACTCAC CCCGCTGGGA CCAGGACGAT 
GAGGACCTGG AGGACCTGGA GGACCTGGAA GATGAGGAGC TGCCCGAGGA GGAGGAGGAG 
TTGGAGGAGG AGGGGGAGGA GGAATTGGAG GAAGAGGATT TGGAGGAGGA GGAGGAGGTG 
CCTGATGACC TGGCCAGCTA CACCCAGCAG GAGGACACCA CTGTGGCTGA GCCCAAAGAG 
TTCAAGCGGA TCAGCTTCCC AACGGCTGCG CCTCAGAAGG ATGACAAGGT TTCAGCTGTG 
CCTACAGAGG CCCCCGAGGT GGCCAAAGGC ATCCCCAAAG CAGCCACACC CGAGGAGAAG 
GCAGCTGCAG AGCGTGCGCA GGAAGCCGAG CCCCCCAAGT CTGAGGAGAG TTTCAGATCT 
CGAGAGGAGG AAGAGGGCCA GGAAGGGCAG GATGCCATGT CCAGAGCCAA AGCCAACTGG 
TTGCGAGCCT TCAACAAGGT GCGCATGCAG CTGCAGGAGG CCCGAGGAGA AGGGGATATG 
TCTAAGTCTC TATGGTTCAA AGGCGGTCCT GGTGGTGGCC TTATCATCAT TGACAGCATG 
CCAGACATCA GGAAACGGAA GCCCATTCCC CTCGTGAGCG ACCTGGCTAT GTCATTGGTG 
CAGTCTCGGA AGGCAGGCAT CACCTCGGCC TTGGCCTCCA GCACGTTGAA CAATGAAGAG 
CTGAAAAATC ACGTTTACAA GAAGACCCTG CAAGCCTTAA TCTACCCCAT CTCCTGCACC 
ACGCCGCACA ATTTCGAGGT GTGGACGGCC ACCACGCCCA CCTACTGCTA TGAGTGCGAG 
GGGCTGCTGT GGGGCATCGC GCGGCAGGGC ATGCGATGCA CCGAGTGCGG CGTTAAGTGC 
CACGAGAAGT GCCAGGACCT GCTCAATGCG GATTGCCTGC AGCGGGCGGC TGAGAAGAGT 
TCCAAGCATG GCGCTGAAGA CCGCACGCAG AACATCATCA TGGTGCTGAA GGACCGCATG 
AAGATCCGCG AGCGCAACAA GCCTGAGATC TTCGAGCTTA TCCAAGAGAT CTTCGCGGTC 
ACCAAGAGCG CACACACACA GCAGATGAAA GCTGTCAAGC AGAGTGTGCT GGACGGCACA 
TCCAAGTGGT CTGCCAAAAT TAGCATCACA GTGGTCTGTG CCCAGGGCTT GCAGGCCAAG 
GACAAGACAG GATCCAGTGA CCCTTATGTC ACCGTCCAGG TTGGGAAGAC CAAGAAAAGG 
ACAAAAACCA TCTACGGGAA CCTCAACCCA GTGTGGGAAG AGAATTTTCA TTTTGAATGT 
CACAACTCCT CTGACCGGAT CAAGGTTCGT GTTTGGGATG AAGATGACGA CATAAAATCC 
CGTGTGAAAC AAAGGTTTAA GAGGGAGTCT GATGACTTCC TAGGGCAGAC AATCATCGAG 
GTGCGGACGC TTAGCGGCGA GATGGATGTG TGGTACAATC TGGACAAGAG AACGGACAAG 
TCGGCGGTGT CGGGGGCCAT CCGGCTTCAC ATCAGTGTGG AGATCAAAGG GGAGGAGAAG 
GTGGCGCCCT ACCATGTCCA GTACACCTGT CTGCATGAGA ACCTGTTCCA CTTTGTGACG 
GACGTGCAGA ACAATGGTGT GGTGAAGATT CCCGATGCCA AGGGTGACGA CGCCTGGAAG 
GTTTACTACG ATGAGACGGC CCAGGAGATC GTGGATGAGT TTGCCATGCG CTATGGTGTT 
GAGTCCATCT ACCAAGCCAT GACCCACTTT GCCTGCCTCT CCTCCAAGTA TATGTGCCCT 
GGGGTACCCG CTGTCATGAG CACCCTGCTT GCCAACATCA ACGCCTACTA CGCACACACC 
ACCGCCTCCA CCAACGTGTC TGCCTCTGAC CGCTTCGCTG CCTCTAATTT CGGGAAAGAG 
CGCTTTGTGA AACTTCTGGA CCAGCTACAC AATTCTCTGC GGATCGACCT GTCCATGTAC 
CGGAACAACT TCCCAGCCAG CAGCCCTGAG CGGCTGCAGG ATCTCAAGTC CACGGTGGAC 
CTGCTCACCA GCATCACCTT CTTCCGGATG AAGGTTCAGG AACTGCAGAG CCCGCCGCGT 
GCCAGCCAGG TAGTGAAAGA CTGTGTGAAG GCCTGCCTCA ACTCCACCTA TGAGTACATC 
TTCAACAACT GCCATGAGCT CTATGGCCGG GAGTACCAAA CGGATCCGGC CAAGAAGGGG 
GAGGTTCCCC CAGAGGAGCA AGGCCCTAGC ATCAAGAACC TGGATTTCTG GTCCAAGCTG 
ATCACCCTCA TCGTGTCTAT CATCGAGGAG GATAAGAATT CTTACACACC CTGCCTCAAT 
CAGTTTCCCC AGGAGCTCAA TGTGGGGAAG ATCAGTGCTG AGGTGATGTG GAGCCTATTT 
GCCCAAGACA TGAAGTACGC CATGGAGGAA CATGACAAAC ACCGGCTGTG TAAGAGCGCA 
GATTACATGA ACCTGCACTT CAAGGTGAAG TGGCTGTACA ATGAGTACGT GGCCGAGCTG 
CCCACCTTCA AGGACCGTGT GCCTGAGTAC CCTGCGTGGT TTGAGCCCTT CGTCATCCAG 
TGGTTGGATG AGAATGAGGA GGTGTCCCGG GACTTCCTGC ATGGTGCCCT CGAGCGGGAC 
AAGAAGGACG GGTTCCAGCA GACCTCAGAG CACGCCCTGT TCTCTTGCTC AGTGGTGGAC 
GTCTTCTCCC AGCTTAACCA GAGTTTCGAG ATCATCAAGA AGCTGGAGTG TCCTGACCCC 
CAGATCGTGG GCCATTACAT GCGGCGTTTT GCCAAGACCA TTAGCAATGT GCTTCTCCAG 
TATGCAGACA TCGTTTCCAA GGACTTCGCC TCCTACTGCT CCAAGGAGAA GGAGAAAGTG 
CCCTGCATCC TCATGAACAA CACACAGCAA CTACGAGTGC AGCTGGAGAA GATGTTTGAG 
GCGATGGGCG GGAAGGAGCT GGATGCCGAG GCCAGTGGCA CCCTGAAGGA GCTGCAGGTG 
AAACTCAACA ATGTCCTGGA TGAACTCAGC CATGTGTTTG CCACCAGCTT CCAGCCACAC 
ATTGAGGAAT GCGTCAGACA GATGGGTGAC ATCCTGAGCC AGGTGAAGGG CACAGGCAAT 
GTACCTGCCA GTGCCTGCAG CAGCGTGGCC CAGGATGCAG ACAATGTGCT GCAGCCCATC 
ATGGATCTTC TGGACAGCAA CCTCACCCTG TTTGCCAAAA TCTGTGAGAA GACAGTTCTG 
AAGCGGGTGC TGAAGGAGCT CTGGAAGCTG GTGATGAACA CAATGGAGAA GACCATCGTC 
CTGCCACCGC TCACTGACCA GACGGGGACA CAAATGATCT TCAATGCTGC CAAGGAGCTG 
GGCCAGCTGT CCAAACTTAA GGATCACATG GTGCGGGAAG AAGCCAAGAG CTTGACCCCG 
AAGCAGTGTG CTGTTGTTGA ACTGGCCCTG GACACCATCA AGCAATACTT CCACGCGGGG 
GGCGTTGGCC TCAAGAAGAC CTTCCTGGAG AAGAGCCCGG ACCTCCAGTC CCTGCGCTAC 
GCCCTGTCGC TCTACACGCA AGCCACCGAC CTGCTCATCA AAACCTTCGT GCAGACGCAG 
TCAGCGCAGG GCTCTGGTGT GGAGGACCCT GTAGGTGAAG TGTCCGTCCA CGTGGAGCTG 
TTCACACATC CAGGAACTGG GGAACAGAAG GTCACAGTGA AGGTGGTGGC CGCCAATGAC 
CTCAAGTGGC AGACTTCTGG CATCTTCCGC CCGTTCATCG AGGTCAACAT CGTTGGACCT 
CAGCTTAGCG ACAAGAAACG CAAGTTCGCC ACCAAATCCA AAAACAACAG CTGGGCGCCC 
AAGTATAACG AGAGCTTCCA GTTCTCCCTG AGCGCCGACG CGGGCCCCGA GTGCTACGAG 
CTGCAGGTGT GCGTGAAGGA CTACTGCTTT GCGCGCGAAG ACCGCACGGT GGGGCTGGCG 
GTGCTGCAAC TGCGAGAGCT GGCCCAGCGC GGGAGCGCCG CGTGCTGGCT TCCACTCGGC 
CGCCGCATCC ACATGGACGA CACGGGGCTC ACAGTGCTGC GCATCCTGTC GCAGCGCAGC 
AACGATGAGG TGGCCAAGGA ATTCGTCAAG CTCAAGTCGG ACACGCGCTC AGCCGAGGAG 
GGCGGTGCCG CGCCTGCGCC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168372.1
CDS	126..5189
Translation	MSLLCVGVKKAKFDGAQEKFNTYVTLKVQNVKSTTIAVRGSQPSWEQDFMFEINRLDLGLTVEVWNKGLIWDTMVGTVWIPLRTIRQSNEEGPGEWLTLDSQAIMADSEICGTKDPTFHRILLDAHFELPLDIPEEEARYWAKKLEQLNAMRDQDEYSFQDQQDKPLPVPSSQCCNWNYFGWGEQNDDPDSAVDDRDSDYRSETSNSIPPPYYTTSQPNASVHQYSVRPPPLGSRESYSDSMHSYEEFSEPRALSPTGSSRYASSGELSQGSSQLSEDFDPDEHSLQGSELDDERDRDSYHSCHSSVSYHKDSPRWDQDDEDLEDLEDLEDEELPEEEEELEEEGEEELEEEDLEEEEEVPDDLASYTQQEDTTVAEPKEFKRISFPTAAPQKDDKVSAVPTEAPEVAKGIPKAATPEEKAAAERAQEAEPPKSEESFRSREEEEGQEGQDAMSRAKANWLRAFNKVRMQLQEARGEGDMSKSLWFKGGPGGGLIIIDSMPDIRKRKPIPLVSDLAMSLVQSRKAGITSALASSTLNNEELKNHVYKKTLQALIYPISCTTPHNFEVWTATTPTYCYECEGLLWGIARQGMRCTECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMVLKDRMKIRERNKPEIFELIQEIFAVTKSAHTQQMKAVKQSVLDGTSKWSAKISITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIYGNLNPVWEENFHFECHNSSDRIKVRVWDEDDDIKSRVKQRFKRESDDFLGQTIIEVRTLSGEMDVWYNLDKRTDKSAVSGAIRLHISVEIKGEEKVAPYHVQYTCLHENLFHFVTDVQNNGVVKIPDAKGDDAWKVYYDETAQEIVDEFAMRYGVESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSMYRNNFPASSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHELYGREYQTDPAKKGEVPPEEQGPSIKNLDFWSKLITLIVSIIEEDKNSYTPCLNQFPQELNVGKISAEVMWSLFAQDMKYAMEEHDKHRLCKSADYMNLHFKVKWLYNEYVAELPTFKDRVPEYPAWFEPFVIQWLDENEEVSRDFLHGALERDKKDGFQQTSEHALFSCSVVDVFSQLNQSFEIIKKLECPDPQIVGHYMRRFAKTISNVLLQYADIVSKDFASYCSKEKEKVPCILMNNTQQLRVQLEKMFEAMGGKELDAEASGTLKELQVKLNNVLDELSHVFATSFQPHIEECVRQMGDILSQVKGTGNVPASACSSVAQDADNVLQPIMDLLDSNLTLFAKICEKTVLKRVLKELWKLVMNTMEKTIVLPPLTDQTGTQMIFNAAKELGQLSKLKDHMVREEAKSLTPKQCAVVELALDTIKQYFHAGGVGLKKTFLEKSPDLQSLRYALSLYTQATDLLIKTFVQTQSAQGSGVEDPVGEVSVHVELFTHPGTGEQKVTVKVVAANDLKWQTSGIFRPFIEVNIVGPQLSDKKRKFATKSKNNSWAPKYNESFQFSLSADAGPECYELQVCVKDYCFAREDRTVGLAVLQLRELAQRGSAACWLPLGRRIHMDDTGLTVLRILSQRSNDEVAKEFVKLKSDTRSAEEGGAAPAP

Target ORF information:

RefSeq Version	XM_017312883.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus unc-13 homolog A (Unc13a), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312883.2

ORF Insert Sequence:

ATGTCTCTGC TGTGCGTGGG AGTCAAAAAA GCCAAGTTTG ACGGTGCCCA AGAGAAGTTC 
AACACATACG TGACGCTGAA GGTGCAGAAC GTGAAGAGCA CTACCATAGC TGTACGTGGC 
AGCCAGCCCA GCTGGGAGCA GGACTTCATG TTTGAGATCA ACCGCCTGGA TCTAGGCCTG 
ACCGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGTAC TGTGTGGATC 
CCGCTGCGGA CCATCCGCCA GTCCAATGAG GAGGGCCCAG GAGAGTGGCT CACACTGGAC 
TCTCAGGCCA TCATGGCGGA CAGTGAGATC TGTGGGACCA AGGACCCCAC CTTCCATCGC 
ATCCTCCTGG ACGCTCATTT CGAGCTGCCG TTGGACATCC CTGAGGAGGA GGCACGCTAC 
TGGGCCAAGA AGCTGGAGCA GCTGAATGCC ATGCGTGACC AGGATGAGTA CTCCTTCCAG 
GACCAGCAGG ACAAGCCGCT GCCAGTGCCC AGCAGCCAGT GCTGCAACTG GAATTACTTT 
GGCTGGGGAG AACAGAATGA CGACCCCGAC AGTGCGGTGG ATGACCGGGA CAGTGACTAT 
AGGAGTGAGA CCAGCAACAG CATCCCACCG CCCTACTATA CGACTTCGCA GCCCAACGCC 
TCTGTGCACC AGTACTCCGT GCGACCGCCC CCTCTGGGGT CCCGGGAGTC CTACAGTGAC 
TCCATGCACA GCTATGAGGA GTTCTCTGAG CCGCGGGCGC TCAGCCCCAC AGGCAGCAGC 
CGCTATGCTT CTTCCGGGGA GCTGAGCCAG GGAAGCTCAC AGCTGAGCGA GGACTTCGAC 
CCTGATGAGC ACAGCCTGCA GGGCTCGGAG CTGGATGACG AGAGGGACCG CGATTCTTAT 
CACTCCTGTC ACAGCTCCGT GAGTTACCAC AAGGACTCAC CCCGCTGGGA CCAGGACGAT 
GAGGACCTGG AGGACCTGGA GGACCTGGAA GATGAGGAGC TGCCCGAGGA GGAGGAGGAG 
TTGGAGGAGG AGGGGGAGGA GGAATTGGAG GAAGAGGATT TGGAGGAGGA GGAGGAGGTG 
CCTGATGACC TGGCCAGCTA CACCCAGCAG GAGGACACCA CTGTGGCTGA GCCCAAAGAG 
TTCAAGCGGA TCAGCTTCCC AACGGCTGCG CCTCAGAAGG ATGACAAGGT TTCAGCTGTG 
CCTACAGAGG CCCCCGAGGT GGCCAAAGGC ATCCCCAAAG CAGCCACACC CGAGGAGAAG 
GCAGCTGCAG AGCGTGCGCA GGAAGCCGAG CCCCCCAAGT CTGAGGAGAG TTTCAGATCT 
CGAGAGGAGG AAGAGGGCCA GGAAGGGCAG GATGCCATGT CCAGAGCCAA AGCCAACTGG 
TTGCGAGCCT TCAACAAGGT GCGCATGCAG CTGCAGGAGG CCCGAGGAGA AGGGGATATG 
TCTAAGTCTC TATGGTTCAA AGGCGGTCCT GGTGGTGGCC TTATCATCAT TGACAGCATG 
CCAGACATCA GGAAACGGAA GCCCATTCCC CTCGTGAGCG ACCTGGCTAT GTCATTGGTG 
CAGTCTCGGA AGGCAGGCAT CACCTCGGCC TTGGCCTCCA GCACGTTGAA CAATGAAGAG 
CTGAAAAATC ACGTTTACAA GAAGACCCTG CAAGCCTTAA TCTACCCCAT CTCCTGCACC 
ACGCCGCACA ATTTCGAGGT GTGGACGGCC ACCACGCCCA CCTACTGCTA TGAGTGCGAG 
GGGCTGCTGT GGGGCATCGC GCGGCAGGGC ATGCGATGCA CCGAGTGCGG CGTTAAGTGC 
CACGAGAAGT GCCAGGACCT GCTCAATGCG GATTGCCTGC AGCGGGCGGC TGAGAAGAGT 
TCCAAGCATG GCGCTGAAGA CCGCACGCAG AACATCATCA TGGTGCTGAA GGACCGCATG 
AAGATCCGCG AGCGCAACAA GCCTGAGATC TTCGAGCTTA TCCAAGAGAT CTTCGCGGTC 
ACCAAGAGCG CACACACACA GCAGATGAAA GCTGTCAAGC AGAGTGTGCT GGACGGCACA 
TCCAAGTGGT CTGCCAAAAT TAGCATCACA GTGGTCTGTG CCCAGGGCTT GCAGGCCAAG 
GACAAGACAG GATCCAGTGA CCCTTATGTC ACCGTCCAGG TTGGGAAGAC CAAGAAAAGG 
ACAAAAACCA TCTACGGGAA CCTCAACCCA GTGTGGGAAG AGAATTTTCA TTTTGAATGT 
CACAACTCCT CTGACCGGAT CAAGGTTCGT GTTTGGGATG AAGATGACGA CATAAAATCC 
CGTGTGAAAC AAAGGTTTAA GAGGGAGTCT GATGACTTCC TAGGGCAGAC AATCATCGAG 
GTGCGGACGC TTAGCGGCGA GATGGATGTG TGGTACAATC TGGACAAGAG AACGGACAAG 
TCGGCGGTGT CGGGGGCCAT CCGGCTTCAC ATCAGTGTGG AGATCAAAGG GGAGGAGAAG 
GTGGCGCCCT ACCATGTCCA GTACACCTGT CTGCATGAGA ACCTGTTCCA CTTTGTGACG 
GACGTGCAGA ACAATGGTGT GGTGAAGATT CCCGATGCCA AGGGTGACGA CGCCTGGAAG 
GTTTACTACG ATGAGACGGC CCAGGAGATC GTGGATGAGT TTGCCATGCG CTATGGTGTT 
GAGTCCATCT ACCAAGCCAT GACCCACTTT GCCTGCCTCT CCTCCAAGTA TATGTGCCCT 
GGGGTACCCG CTGTCATGAG CACCCTGCTT GCCAACATCA ACGCCTACTA CGCACACACC 
ACCGCCTCCA CCAACGTGTC TGCCTCTGAC CGCTTCGCTG CCTCTAATTT CGGGAAAGAG 
CGCTTTGTGA AACTTCTGGA CCAGCTACAC AATTCTCTGC GGATCGACCT GTCCATGTAC 
CGGAACAACT TCCCAGCCAG CAGCCCTGAG CGGCTGCAGG ATCTCAAGTC CACGGTGGAC 
CTGCTCACCA GCATCACCTT CTTCCGGATG AAGGTTCAGG AACTGCAGAG CCCGCCGCGT 
GCCAGCCAGG TAGTGAAAGA CTGTGTGAAG GCCTGCCTCA ACTCCACCTA TGAGTACATC 
TTCAACAACT GCCATGAGCT CTATGGCCGG GAGTACCAAA CGGATCCGGC CAAGAAGGGG 
GAGGTTCCCC CAGAGGAGCA AGGCCCTAGC ATCAAGAACC TGGATTTCTG GTCCAAGCTG 
ATCACCCTCA TCGTGTCTAT CATCGAGGAG GATAAGAATT CTTACACACC CTGCCTCAAT 
CAGTTTCCCC AGGAGCTCAA TGTGGGGAAG ATCAGTGCTG AGGTGATGTG GAGCCTATTT 
GCCCAAGACA TGAAGTACGC CATGGAGGAA CATGACAAAC ACCGGCTGTG TAAGAGCGCA 
GATTACATGA ACCTGCACTT CAAGGTGAAG TGGCTGTACA ATGAGTACGT GGCCGAGCTG 
CCCACCTTCA AGGACCGTGT GCCTGAGTAC CCTGCGTGGT TTGAGCCCTT CGTCATCCAG 
TGGTTGGATG AGAATGAGGA GGTGTCCCGG GACTTCCTGC ATGGTGCCCT CGAGCGGGAC 
AAGAAGGACG GGTTCCAGCA GACCTCAGAG CACGCCCTGT TCTCTTGCTC AGTGGTGGAC 
GTCTTCTCCC AGCTTAACCA GAGTTTCGAG ATCATCAAGA AGCTGGAGTG TCCTGACCCC 
CAGATCGTGG GCCATTACAT GCGGCGTTTT GCCAAGACCA TTAGCAATGT GCTTCTCCAG 
TATGCAGACA TCGTTTCCAA GGACTTCGCC TCCTACTGCT CCAAGGAGAA GGAGAAAGTG 
CCCTGCATCC TCATGAACAA CACACAGCAA CTACGAGTGC AGCTGGAGAA GATGTTTGAG 
GCGATGGGCG GGAAGGAGCT GGATGCCGAG GCCAGTGGCA CCCTGAAGGA GCTGCAGGTG 
AAACTCAACA ATGTCCTGGA TGAACTCAGC CATGTGTTTG CCACCAGCTT CCAGCCACAC 
ATTGAGGAAT GCGTCAGACA GATGGGTGAC ATCCTGAGCC AGGTGAAGGG CACAGGCAAT 
GTACCTGCCA GTGCCTGCAG CAGCGTGGCC CAGGATGCAG ACAATGTGCT GCAGCCCATC 
ATGGATCTTC TGGACAGCAA CCTCACCCTG TTTGCCAAAA TCTGTGAGAA GACAGTTCTG 
AAGCGGGTGC TGAAGGAGCT CTGGAAGCTG GTGATGAACA CAATGGAGAA GACCATCGTC 
CTGCCACCGC TCACTGACCA GACGGGGACA CAAATGATCT TCAATGCTGC CAAGGAGCTG 
GGCCAGCTGT CCAAACTTAA GGATCACATG GTGCGGGAAG AAGCCAAGAG CTTGACCCCG 
AAGCAGTGTG CTGTTGTTGA ACTGGCCCTG GACACCATCA AGCAATACTT CCACGCGGGG 
GGCGTTGGCC TCAAGAAGAC CTTCCTGGAG AAGAGCCCGG ACCTCCAGTC CCTGCGCTAC 
GCCCTGTCGC TCTACACGCA AGCCACCGAC CTGCTCATCA AAACCTTCGT GCAGACGCAG 
TCAGCGCAGG GCTCTGGTGT GGAGGACCCT GTAGGTGAAG TGTCCGTCCA CGTGGAGCTG 
TTCACACATC CAGGAACTGG GGAACAGAAG GTCACAGTGA AGGTGGTGGC CGCCAATGAC 
CTCAAGTGGC AGACTTCTGG CATCTTCCGC CCGTTCATCG AGGTCAACAT CGTTGGACCT 
CAGCTTAGCG ACAAGAAACG CAAGTTCGCC ACCAAATCCA AAAACAACAG CTGGGCGCCC 
AAGTATAACG AGAGCTTCCA GTTCTCCCTG AGCGCCGACG CGGGCCCCGA GTGCTACGAG 
CTGCAGGTGT GCGTGAAGGA CTACTGCTTT GCGCGCGAAG ACCGCACGGT GGGGCTGGCG 
GTGCTGCAAC TGCGAGAGCT GGCCCAGCGC GGGAGCGCCG CGTGCTGGCT TCCACTCGGC 
CGCCGCATCC ACATGGACGA CACGGGGCTC ACAGTGCTGC GCATCCTGTC GCAGCGCAGC 
AACGATGAGG TGGCCAAGGA ATTCGTCAAG CTCAAGTCGG ACACGCGCTC AGCCGAGGAG 
GGCGGTGCCG CGCCTGCGCC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Unc13a ( XM_030243646.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030099506.1 Mus musculus unc-13 homolog A (Unc13a), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMu89888
Clone ID Related Accession (Same CDS sequence)	XM_030243646.1
Accession Version	XM_030243646.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5166bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein unc-13 homolog A isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTGC TGTGCGTGGG AGAGAAGTTC AACACATACG TGACGCTGAA GGTGCAGAAC 
GTGAAGAGCA CTACCATAGC TGTACGTGGC AGCCAGCCCA GCTGGGAGCA GGACTTCATG 
TTTGAGATCA ACCGCCTGGA TCTAGGCCTG ACCGTGGAGG TGTGGAACAA GGGTCTCATC 
TGGGACACGA TGGTGGGTAC TGTGTGGATC CCGCTGCGGA CCATCCGCCA GTCCAATGAG 
GAGGGCCCAG GAGAGTGGCT CACACTGGAC TCTCAGGCCA TCATGGCGGA CAGTGAGATC 
TGTGGGACCA AGGACCCCAC CTTCCATCGC ATCCTCCTGG ACGCTCATTT CGAGCTGCCG 
TTGGACATCC CTGAGGAGGA GGCACGCTAC TGGGCCAAGA AGCTGGAGCA GCTGAATGCC 
ATGCGTGACC AGGATGAGTA CTCCTTCCAG GACCAGCAGG ACAAGCCGCT GCCAGTGCCC 
AGCAGCCAGT GCTGCAACTG GAATTACTTT GGCTGGGGAG AACAGAATGA CGACCCCGAC 
AGTGCGGTGG ATGACCGGGA CAGTGACTAT AGGAGTGAGA CCAGCAACAG CATCCCACCG 
CCCTACTATA CGACTTCGCA GCCCAACGCC TCTGTGCACC AGTACTCCGT GCGACCGCCC 
CCTCTGGGGT CCCGGGAGTC CTACAGTGAC TCCATGCACA GCTATGAGGA GTTCTCTGAG 
CCGCGGGCGC TCAGCCCCAC AGGCAGCAGC CGCTATGCTT CTTCCGGGGA GCTGAGCCAG 
GGAAGCTCAC AGCTGAGCGA GGACTTCGAC CCTGATGAGC ACAGCCTGCA GGGCTCGGAG 
CTGGATGACG AGAGGGACCG CGATTCTTAT CACTCCTGTC ACAGCTCCGT GAGTTACCAC 
AAGGACTCAC CCCGCTGGGA CCAGGACGAT GAGGACCTGG AGGACCTGGA GGACCTGGAA 
GATGAGGAGC TGCCCGAGGA GGAGGAGGAG TTGGAGGAGG AGGGGGAGGA GGAATTGGAG 
GAAGAGGATT TGGAGGAGGA GGAGGAGGTG CCTGATGACC TGGCCAGCTA CACCCAGCAG 
GAGGACACCA CTGTGGCTGA GCCCAAAGAG TTCAAGCGGA TCAGCTTCCC AACGGCTGCG 
CCTCAGAAGG ATGACAAGGT TTCAGCTGTG CCTACAGAGG CCCCCGAGGT GGCCAAAGGC 
ATCCCCAAAG CAGCCACACC CGAGGAGAAG GCAGCTGCAG AGCGTGCGCA GGAAGCCGAG 
CCCCCCAAGT CTGAGGAGAG TTTCAGATCT CGAGAGGAGG AAGAGGGCCA GGAAGGGCAG 
GATGCCATGT CCAGAGCCAA AGCCAACTGG TTGCGAGCCT TCAACAAGGT GCGCATGCAG 
CTGCAGGAGG CCCGAGGAGA AGGGGATATG TCTAAGTCTC TATGGTTCAA AGGCGGTCCT 
GGTGGTGGCC TTATCATCAT TGACAGCATG CCAGACATCA GGAAACGGAA GCCCATTCCC 
CTCGTGAGCG ACCTGGCTAT GTCATTGGTG CAGTCTCGGA AGGCAGGCAT CACCTCGGCC 
TTGGCCTCCA GCACGTTGAA CAATGAAGAG CTGAAAAATC ACGTTTACAA GAAGACCCTG 
CAAGCCTTAA TCTACCCCAT CTCCTGCACC ACGCCGCACA ATTTCGAGGT GTGGACGGCC 
ACCACGCCCA CCTACTGCTA TGAGTGCGAG GGGCTGCTGT GGGGCATCGC GCGGCAGGGC 
ATGCGATGCA CCGAGTGCGG CGTTAAGTGC CACGAGAAGT GCCAGGACCT GCTCAATGCG 
GATTGCCTGC AGCGGGCGGC TGAGAAGAGT TCCAAGCATG GCGCTGAAGA CCGCACGCAG 
AACATCATCA TGGTGCTGAA GGACCGCATG AAGATCCGCG AGCGCAACAA GCCTGAGATC 
TTCGAGCTTA TCCAAGAGAT CTTCGCGGTC ACCAAGAGCG CACACACACA GCAGATGAAA 
GCTGTCAAGC AGAGTGTGCT GGACGGCACA TCCAAGTGGT CTGCCAAAAT TAGCATCACA 
GTGGTCTGTG CCCAGGGCTT GCAGGCCAAG GACAAGACAG GATCCAGTGA CCCTTATGTC 
ACCGTCCAGG TTGGGAAGAC CAAGAAAAGG ACAAAAACCA TCTACGGGAA CCTCAACCCA 
GTGTGGGAAG AGAATTTTCA TTTTGAATGT CACAACTCCT CTGACCGGAT CAAGGTTCGT 
GTTTGGGATG AAGATGACGA CATAAAATCC CGTGTGAAAC AAAGGTTTAA GAGGGAGTCT 
GATGACTTCC TAGGGCAGAC AATCATCGAG GTGCGGACGC TTAGCGGCGA GATGGATGTG 
TGGTACAATC TGGACAAGAG AACGGACAAG TCGGCGGTGT CGGGGGCCAT CCGGCTTCAC 
ATCAGTGTGG AGATCAAAGG GGAGGAGAAG GTGGCGCCCT ACCATGTCCA GTACACCTGT 
CTGCATGAGA ACCTGTTCCA CTTTGTGACG GACGTGCAGA ACAATGGTGT GGTGAAGATT 
CCCGATGCCA AGGGTGACGA CGCCTGGAAG GTTTACTACG ATGAGACGGC CCAGGAGATC 
GTGGATGAGT TTGCCATGCG CTATGGTGTT GAGTCCATCT ACCAAGCCAT GACCCACTTT 
GCCTGCCTCT CCTCCAAGTA TATGTGCCCT GGGGTACCCG CTGTCATGAG CACCCTGCTT 
GCCAACATCA ACGCCTACTA CGCACACACC ACCGCCTCCA CCAACGTGTC TGCCTCTGAC 
CGCTTCGCTG CCTCTAATTT CGGGAAAGAG CGCTTTGTGA AACTTCTGGA CCAGCTACAC 
AATTCTCTGC GGATCGACCT GTCCATGTAC CGGAACAACT TCCCAGCCAG CAGCCCTGAG 
CGGCTGCAGG ATCTCAAGTC CACGGTGGAC CTGCTCACCA GCATCACCTT CTTCCGGATG 
AAGGTTCAGG AACTGCAGAG CCCGCCGCGT GCCAGCCAGG TAGTGAAAGA CTGTGTGAAG 
GCCTGCCTCA ACTCCACCTA TGAGTACATC TTCAACAACT GCCATGAGCT CTATGGCCGG 
GAGTACCAAA CGGATCCGGC CAAGAAGGGG GAGGTTCCCC CAGAGGAGCA AGGCCCTAGC 
ATCAAGAACC TGGATTTCTG GTCCAAGCTG ATCACCCTCA TCGTGTCTAT CATCGAGGAG 
GATAAGAATT CTTACACACC CTGCCTCAAT CAGTTTCCCC AGGAGCTCAA TGTGGGGAAG 
ATCAGTGCTG AGGTGATGTG GAGCCTATTT GCCCAAGACA TGAAGTACGC CATGGAGGAA 
CATGACAAAC ACCGGCTGTG TAAGAGCGCA GATTACATGA ACCTGCACTT CAAGGTGAAG 
TGGCTGTACA ATGAGTACGT GGCCGAGCTG CCCACCTTCA AGGACCGTGT GCCTGAGTAC 
CCTGCGTGGT TTGAGCCCTT CGTCATCCAG TGGTTGGATG AGAATGAGGA GGTGTCCCGG 
GACTTCCTGC ATGGTGCCCT CGAGCGGGAC AAGAAGGACG GGTTCCAGCA GACCTCAGAG 
CACGCCCTGT TCTCTTGCTC AGTGGTGGAC GTCTTCTCCC AGCTTAACCA GAGTTTCGAG 
ATCATCAAGA AGCTGGAGTG TCCTGACCCC CAGATCGTGG GCCATTACAT GCGGCGTTTT 
GCCAAGACCA TTAGCAATGT GCTTCTCCAG TATGCAGACA TCGTTTCCAA GGACTTCGCC 
TCCTACTGCT CCAAGGAGAA GGAGAAAGTG CCCTGCATCC TCATGAACAA CACACAGCAA 
CTACGAGTGC AGCTGGAGAA GATGTTTGAG GCGATGGGCG GGAAGGAGCT GGATGCCGAG 
GCCAGTGGCA CCCTGAAGGA GCTGCAGGTG AAACTCAACA ATGTCCTGGA TGAACTCAGC 
CATGTGTTTG CCACCAGCTT CCAGCCACAC ATTGAGGAAT GCGTCAGACA GATGGGTGAC 
ATCCTGAGCC AGGTGAAGGG CACAGGCAAT GTACCTGCCA GTGCCTGCAG CAGCGTGGCC 
CAGGATGCAG ACAATGTGCT GCAGCCCATC ATGGATCTTC TGGACAGCAA CCTCACCCTG 
TTTGCCAAAA TCTGTGAGAA GACAGTTCTG AAGCGGGTGC TGAAGGAGCT CTGGAAGCTG 
GTGATGAACA CAATGGAGAA GACCATCGTC CTGCCACCGC TCACTGACCA GACGATGATT 
GGCACCCTCT TGAGAAAACA TGGCAAGGGC CTAGAAAAGG GCAGGGTGAA ACTGCCAAGC 
CACTCAGACG GGACACAAAT GATCTTCAAT GCTGCCAAGG AGCTGGGCCA GCTGTCCAAA 
CTTAAGGATC ACATGGTGCG GGAAGAAGCC AAGAGCTTGA CCCCGAAGCA GTGTGCTGTT 
GTTGAACTGG CCCTGGACAC CATCAAGCAA TACTTCCACG CGGGGGGCGT TGGCCTCAAG 
AAGACCTTCC TGGAGAAGAG CCCGGACCTC CAGTCCCTGC GCTACGCCCT GTCGCTCTAC 
ACGCAAGCCA CCGACCTGCT CATCAAAACC TTCGTGCAGA CGCAGTCAGC GCAGGTCCAT 
GGTGGAAAAG GGACTAGGTT TACCCTTAGT GAAGACGTTT GTCCTGAGAT GGGCTCTGGT 
GTGGAGGACC CTGTAGGTGA AGTGTCCGTC CACGTGGAGC TGTTCACACA TCCAGGAACT 
GGGGAACAGA AGGTCACAGT GAAGGTGGTG GCCGCCAATG ACCTCAAGTG GCAGACTTCT 
GGCATCTTCC GCCCGTTCAT CGAGGTCAAC ATCGTTGGAC CTCAGCTTAG CGACAAGAAA 
CGCAAGTTCG CCACCAAATC CAAAAACAAC AGCTGGGCGC CCAAGTATAA CGAGAGCTTC 
CAGTTCTCCC TGAGCGCCGA CGCGGGCCCC GAGTGCTACG AGCTGCAGGT GTGCGTGAAG 
GACTACTGCT TTGCGCGCGA AGACCGCACG GTGGGGCTGG CGGTGCTGCA ACTGCGAGAG 
CTGGCCCAGC GCGGGAGCGC CGCGTGCTGG CTTCCACTCG GCCGCCGCAT CCACATGGAC 
GACACGGGGC TCACAGTGCT GCGCATCCTG TCGCAGCGCA GCAACGATGA GGTGGCCAAG 
GAATTCGTCA AGCTCAAGTC GGACACGCGC TCAGCCGAGG AGGGCGGTGC CGCGCCTGCG 
CCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030099506.1
CDS	127..5292
Translation	MSLLCVGEKFNTYVTLKVQNVKSTTIAVRGSQPSWEQDFMFEINRLDLGLTVEVWNKGLIWDTMVGTVWIPLRTIRQSNEEGPGEWLTLDSQAIMADSEICGTKDPTFHRILLDAHFELPLDIPEEEARYWAKKLEQLNAMRDQDEYSFQDQQDKPLPVPSSQCCNWNYFGWGEQNDDPDSAVDDRDSDYRSETSNSIPPPYYTTSQPNASVHQYSVRPPPLGSRESYSDSMHSYEEFSEPRALSPTGSSRYASSGELSQGSSQLSEDFDPDEHSLQGSELDDERDRDSYHSCHSSVSYHKDSPRWDQDDEDLEDLEDLEDEELPEEEEELEEEGEEELEEEDLEEEEEVPDDLASYTQQEDTTVAEPKEFKRISFPTAAPQKDDKVSAVPTEAPEVAKGIPKAATPEEKAAAERAQEAEPPKSEESFRSREEEEGQEGQDAMSRAKANWLRAFNKVRMQLQEARGEGDMSKSLWFKGGPGGGLIIIDSMPDIRKRKPIPLVSDLAMSLVQSRKAGITSALASSTLNNEELKNHVYKKTLQALIYPISCTTPHNFEVWTATTPTYCYECEGLLWGIARQGMRCTECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMVLKDRMKIRERNKPEIFELIQEIFAVTKSAHTQQMKAVKQSVLDGTSKWSAKISITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIYGNLNPVWEENFHFECHNSSDRIKVRVWDEDDDIKSRVKQRFKRESDDFLGQTIIEVRTLSGEMDVWYNLDKRTDKSAVSGAIRLHISVEIKGEEKVAPYHVQYTCLHENLFHFVTDVQNNGVVKIPDAKGDDAWKVYYDETAQEIVDEFAMRYGVESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSMYRNNFPASSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHELYGREYQTDPAKKGEVPPEEQGPSIKNLDFWSKLITLIVSIIEEDKNSYTPCLNQFPQELNVGKISAEVMWSLFAQDMKYAMEEHDKHRLCKSADYMNLHFKVKWLYNEYVAELPTFKDRVPEYPAWFEPFVIQWLDENEEVSRDFLHGALERDKKDGFQQTSEHALFSCSVVDVFSQLNQSFEIIKKLECPDPQIVGHYMRRFAKTISNVLLQYADIVSKDFASYCSKEKEKVPCILMNNTQQLRVQLEKMFEAMGGKELDAEASGTLKELQVKLNNVLDELSHVFATSFQPHIEECVRQMGDILSQVKGTGNVPASACSSVAQDADNVLQPIMDLLDSNLTLFAKICEKTVLKRVLKELWKLVMNTMEKTIVLPPLTDQTMIGTLLRKHGKGLEKGRVKLPSHSDGTQMIFNAAKELGQLSKLKDHMVREEAKSLTPKQCAVVELALDTIKQYFHAGGVGLKKTFLEKSPDLQSLRYALSLYTQATDLLIKTFVQTQSAQVHGGKGTRFTLSEDVCPEMGSGVEDPVGEVSVHVELFTHPGTGEQKVTVKVVAANDLKWQTSGIFRPFIEVNIVGPQLSDKKRKFATKSKNNSWAPKYNESFQFSLSADAGPECYELQVCVKDYCFAREDRTVGLAVLQLRELAQRGSAACWLPLGRRIHMDDTGLTVLRILSQRSNDEVAKEFVKLKSDTRSAEEGGAAPAP

Target ORF information:

RefSeq Version	XM_030243646.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus unc-13 homolog A (Unc13a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030243646.1

ORF Insert Sequence:

ATGTCTCTGC TGTGCGTGGG AGAGAAGTTC AACACATACG TGACGCTGAA GGTGCAGAAC 
GTGAAGAGCA CTACCATAGC TGTACGTGGC AGCCAGCCCA GCTGGGAGCA GGACTTCATG 
TTTGAGATCA ACCGCCTGGA TCTAGGCCTG ACCGTGGAGG TGTGGAACAA GGGTCTCATC 
TGGGACACGA TGGTGGGTAC TGTGTGGATC CCGCTGCGGA CCATCCGCCA GTCCAATGAG 
GAGGGCCCAG GAGAGTGGCT CACACTGGAC TCTCAGGCCA TCATGGCGGA CAGTGAGATC 
TGTGGGACCA AGGACCCCAC CTTCCATCGC ATCCTCCTGG ACGCTCATTT CGAGCTGCCG 
TTGGACATCC CTGAGGAGGA GGCACGCTAC TGGGCCAAGA AGCTGGAGCA GCTGAATGCC 
ATGCGTGACC AGGATGAGTA CTCCTTCCAG GACCAGCAGG ACAAGCCGCT GCCAGTGCCC 
AGCAGCCAGT GCTGCAACTG GAATTACTTT GGCTGGGGAG AACAGAATGA CGACCCCGAC 
AGTGCGGTGG ATGACCGGGA CAGTGACTAT AGGAGTGAGA CCAGCAACAG CATCCCACCG 
CCCTACTATA CGACTTCGCA GCCCAACGCC TCTGTGCACC AGTACTCCGT GCGACCGCCC 
CCTCTGGGGT CCCGGGAGTC CTACAGTGAC TCCATGCACA GCTATGAGGA GTTCTCTGAG 
CCGCGGGCGC TCAGCCCCAC AGGCAGCAGC CGCTATGCTT CTTCCGGGGA GCTGAGCCAG 
GGAAGCTCAC AGCTGAGCGA GGACTTCGAC CCTGATGAGC ACAGCCTGCA GGGCTCGGAG 
CTGGATGACG AGAGGGACCG CGATTCTTAT CACTCCTGTC ACAGCTCCGT GAGTTACCAC 
AAGGACTCAC CCCGCTGGGA CCAGGACGAT GAGGACCTGG AGGACCTGGA GGACCTGGAA 
GATGAGGAGC TGCCCGAGGA GGAGGAGGAG TTGGAGGAGG AGGGGGAGGA GGAATTGGAG 
GAAGAGGATT TGGAGGAGGA GGAGGAGGTG CCTGATGACC TGGCCAGCTA CACCCAGCAG 
GAGGACACCA CTGTGGCTGA GCCCAAAGAG TTCAAGCGGA TCAGCTTCCC AACGGCTGCG 
CCTCAGAAGG ATGACAAGGT TTCAGCTGTG CCTACAGAGG CCCCCGAGGT GGCCAAAGGC 
ATCCCCAAAG CAGCCACACC CGAGGAGAAG GCAGCTGCAG AGCGTGCGCA GGAAGCCGAG 
CCCCCCAAGT CTGAGGAGAG TTTCAGATCT CGAGAGGAGG AAGAGGGCCA GGAAGGGCAG 
GATGCCATGT CCAGAGCCAA AGCCAACTGG TTGCGAGCCT TCAACAAGGT GCGCATGCAG 
CTGCAGGAGG CCCGAGGAGA AGGGGATATG TCTAAGTCTC TATGGTTCAA AGGCGGTCCT 
GGTGGTGGCC TTATCATCAT TGACAGCATG CCAGACATCA GGAAACGGAA GCCCATTCCC 
CTCGTGAGCG ACCTGGCTAT GTCATTGGTG CAGTCTCGGA AGGCAGGCAT CACCTCGGCC 
TTGGCCTCCA GCACGTTGAA CAATGAAGAG CTGAAAAATC ACGTTTACAA GAAGACCCTG 
CAAGCCTTAA TCTACCCCAT CTCCTGCACC ACGCCGCACA ATTTCGAGGT GTGGACGGCC 
ACCACGCCCA CCTACTGCTA TGAGTGCGAG GGGCTGCTGT GGGGCATCGC GCGGCAGGGC 
ATGCGATGCA CCGAGTGCGG CGTTAAGTGC CACGAGAAGT GCCAGGACCT GCTCAATGCG 
GATTGCCTGC AGCGGGCGGC TGAGAAGAGT TCCAAGCATG GCGCTGAAGA CCGCACGCAG 
AACATCATCA TGGTGCTGAA GGACCGCATG AAGATCCGCG AGCGCAACAA GCCTGAGATC 
TTCGAGCTTA TCCAAGAGAT CTTCGCGGTC ACCAAGAGCG CACACACACA GCAGATGAAA 
GCTGTCAAGC AGAGTGTGCT GGACGGCACA TCCAAGTGGT CTGCCAAAAT TAGCATCACA 
GTGGTCTGTG CCCAGGGCTT GCAGGCCAAG GACAAGACAG GATCCAGTGA CCCTTATGTC 
ACCGTCCAGG TTGGGAAGAC CAAGAAAAGG ACAAAAACCA TCTACGGGAA CCTCAACCCA 
GTGTGGGAAG AGAATTTTCA TTTTGAATGT CACAACTCCT CTGACCGGAT CAAGGTTCGT 
GTTTGGGATG AAGATGACGA CATAAAATCC CGTGTGAAAC AAAGGTTTAA GAGGGAGTCT 
GATGACTTCC TAGGGCAGAC AATCATCGAG GTGCGGACGC TTAGCGGCGA GATGGATGTG 
TGGTACAATC TGGACAAGAG AACGGACAAG TCGGCGGTGT CGGGGGCCAT CCGGCTTCAC 
ATCAGTGTGG AGATCAAAGG GGAGGAGAAG GTGGCGCCCT ACCATGTCCA GTACACCTGT 
CTGCATGAGA ACCTGTTCCA CTTTGTGACG GACGTGCAGA ACAATGGTGT GGTGAAGATT 
CCCGATGCCA AGGGTGACGA CGCCTGGAAG GTTTACTACG ATGAGACGGC CCAGGAGATC 
GTGGATGAGT TTGCCATGCG CTATGGTGTT GAGTCCATCT ACCAAGCCAT GACCCACTTT 
GCCTGCCTCT CCTCCAAGTA TATGTGCCCT GGGGTACCCG CTGTCATGAG CACCCTGCTT 
GCCAACATCA ACGCCTACTA CGCACACACC ACCGCCTCCA CCAACGTGTC TGCCTCTGAC 
CGCTTCGCTG CCTCTAATTT CGGGAAAGAG CGCTTTGTGA AACTTCTGGA CCAGCTACAC 
AATTCTCTGC GGATCGACCT GTCCATGTAC CGGAACAACT TCCCAGCCAG CAGCCCTGAG 
CGGCTGCAGG ATCTCAAGTC CACGGTGGAC CTGCTCACCA GCATCACCTT CTTCCGGATG 
AAGGTTCAGG AACTGCAGAG CCCGCCGCGT GCCAGCCAGG TAGTGAAAGA CTGTGTGAAG 
GCCTGCCTCA ACTCCACCTA TGAGTACATC TTCAACAACT GCCATGAGCT CTATGGCCGG 
GAGTACCAAA CGGATCCGGC CAAGAAGGGG GAGGTTCCCC CAGAGGAGCA AGGCCCTAGC 
ATCAAGAACC TGGATTTCTG GTCCAAGCTG ATCACCCTCA TCGTGTCTAT CATCGAGGAG 
GATAAGAATT CTTACACACC CTGCCTCAAT CAGTTTCCCC AGGAGCTCAA TGTGGGGAAG 
ATCAGTGCTG AGGTGATGTG GAGCCTATTT GCCCAAGACA TGAAGTACGC CATGGAGGAA 
CATGACAAAC ACCGGCTGTG TAAGAGCGCA GATTACATGA ACCTGCACTT CAAGGTGAAG 
TGGCTGTACA ATGAGTACGT GGCCGAGCTG CCCACCTTCA AGGACCGTGT GCCTGAGTAC 
CCTGCGTGGT TTGAGCCCTT CGTCATCCAG TGGTTGGATG AGAATGAGGA GGTGTCCCGG 
GACTTCCTGC ATGGTGCCCT CGAGCGGGAC AAGAAGGACG GGTTCCAGCA GACCTCAGAG 
CACGCCCTGT TCTCTTGCTC AGTGGTGGAC GTCTTCTCCC AGCTTAACCA GAGTTTCGAG 
ATCATCAAGA AGCTGGAGTG TCCTGACCCC CAGATCGTGG GCCATTACAT GCGGCGTTTT 
GCCAAGACCA TTAGCAATGT GCTTCTCCAG TATGCAGACA TCGTTTCCAA GGACTTCGCC 
TCCTACTGCT CCAAGGAGAA GGAGAAAGTG CCCTGCATCC TCATGAACAA CACACAGCAA 
CTACGAGTGC AGCTGGAGAA GATGTTTGAG GCGATGGGCG GGAAGGAGCT GGATGCCGAG 
GCCAGTGGCA CCCTGAAGGA GCTGCAGGTG AAACTCAACA ATGTCCTGGA TGAACTCAGC 
CATGTGTTTG CCACCAGCTT CCAGCCACAC ATTGAGGAAT GCGTCAGACA GATGGGTGAC 
ATCCTGAGCC AGGTGAAGGG CACAGGCAAT GTACCTGCCA GTGCCTGCAG CAGCGTGGCC 
CAGGATGCAG ACAATGTGCT GCAGCCCATC ATGGATCTTC TGGACAGCAA CCTCACCCTG 
TTTGCCAAAA TCTGTGAGAA GACAGTTCTG AAGCGGGTGC TGAAGGAGCT CTGGAAGCTG 
GTGATGAACA CAATGGAGAA GACCATCGTC CTGCCACCGC TCACTGACCA GACGATGATT 
GGCACCCTCT TGAGAAAACA TGGCAAGGGC CTAGAAAAGG GCAGGGTGAA ACTGCCAAGC 
CACTCAGACG GGACACAAAT GATCTTCAAT GCTGCCAAGG AGCTGGGCCA GCTGTCCAAA 
CTTAAGGATC ACATGGTGCG GGAAGAAGCC AAGAGCTTGA CCCCGAAGCA GTGTGCTGTT 
GTTGAACTGG CCCTGGACAC CATCAAGCAA TACTTCCACG CGGGGGGCGT TGGCCTCAAG 
AAGACCTTCC TGGAGAAGAG CCCGGACCTC CAGTCCCTGC GCTACGCCCT GTCGCTCTAC 
ACGCAAGCCA CCGACCTGCT CATCAAAACC TTCGTGCAGA CGCAGTCAGC GCAGGTCCAT 
GGTGGAAAAG GGACTAGGTT TACCCTTAGT GAAGACGTTT GTCCTGAGAT GGGCTCTGGT 
GTGGAGGACC CTGTAGGTGA AGTGTCCGTC CACGTGGAGC TGTTCACACA TCCAGGAACT 
GGGGAACAGA AGGTCACAGT GAAGGTGGTG GCCGCCAATG ACCTCAAGTG GCAGACTTCT 
GGCATCTTCC GCCCGTTCAT CGAGGTCAAC ATCGTTGGAC CTCAGCTTAG CGACAAGAAA 
CGCAAGTTCG CCACCAAATC CAAAAACAAC AGCTGGGCGC CCAAGTATAA CGAGAGCTTC 
CAGTTCTCCC TGAGCGCCGA CGCGGGCCCC GAGTGCTACG AGCTGCAGGT GTGCGTGAAG 
GACTACTGCT TTGCGCGCGA AGACCGCACG GTGGGGCTGG CGGTGCTGCA ACTGCGAGAG 
CTGGCCCAGC GCGGGAGCGC CGCGTGCTGG CTTCCACTCG GCCGCCGCAT CCACATGGAC 
GACACGGGGC TCACAGTGCT GCGCATCCTG TCGCAGCGCA GCAACGATGA GGTGGCCAAG 
GAATTCGTCA AGCTCAAGTC GGACACGCGC TCAGCCGAGG AGGGCGGTGC CGCGCCTGCG 
CCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Unc13a ( NM_001029873.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001025044.2 Mus musculus unc-13 homolog A (Unc13a), mRNA.

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CloneID	OMu12297
Clone ID Related Accession (Same CDS sequence)	NM_001029873.2
Accession Version	NM_001029873.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5139bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-28
Organism	Mus musculus(house mouse)
Product	protein unc-13 homolog A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ106055.1, AK147408.1, CB250050.1 and CJ250255.1. On Jan 14, 2010 this sequence version replaced NM_001029873.1. CCDS Note: The coding region has been updated to represent a splice pattern that is more supported by the available transcript and protein data. The update results in a protein that is shorter, changes the splice pattern reflected by the CDS, and changes the sequence of the protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY753536.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTCTGC TGTGCGTGGG AGTCAAAAAA GCCAAGTTTG ACGGTGCCCA AGAGAAGTTC 
AACACATACG TGACGCTGAA GGTGCAGAAC GTGAAGAGCA CTACCATAGC TGTACGTGGC 
AGCCAGCCCA GCTGGGAGCA GGACTTCATG TTTGAGATCA ACCGCCTGGA TCTAGGCCTG 
ACCGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGTAC TGTGTGGATC 
CCGCTGCGGA CCATCCGCCA GTCCAATGAG GAGGGCCCAG GAGAGTGGCT CACACTGGAC 
TCTCAGGCCA TCATGGCGGA CAGTGAGATC TGTGGGACCA AGGACCCCAC CTTCCATCGC 
ATCCTCCTGG ACGCTCATTT CGAGCTGCCG TTGGACATCC CTGAGGAGGA GGCACGCTAC 
TGGGCCAAGA AGCTGGAGCA GCTGAATGCC ATGCGTGACC AGGATGAGTA CTCCTTCCAG 
GACCAGCAGG ACAAGCCGCT GCCAGTGCCC AGCAGCCAGT GCTGCAACTG GAATTACTTT 
GGCTGGGGAG AACAGAATGA CGACCCCGAC AGTGCGGTGG ATGACCGGGA CAGTGACTAT 
AGGAGTGAGA CCAGCAACAG CATCCCACCG CCCTACTATA CGACTTCGCA GCCCAACGCC 
TCTGTGCACC AGTACTCCGT GCGACCGCCC CCTCTGGGGT CCCGGGAGTC CTACAGTGAC 
TCCATGCACA GCTATGAGGA GTTCTCTGAG CCGCGGGCGC TCAGCCCCAC AGGCAGCAGC 
CGCTATGCTT CTTCCGGGGA GCTGAGCCAG GGAAGCTCAC AGCTGAGCGA GGACTTCGAC 
CCTGATGAGC ACAGCCTGCA GGGCTCGGAG CTGGATGACG AGAGGGACCG CGATTCTTAT 
CACTCCTGTC ACAGCTCCGT GAGTTACCAC AAGGACTCAC CCCGCTGGGA CCAGGACGAT 
GAGGACCTGG AGGACCTGGA GGACCTGGAA GATGAGGAGC TGCCCGAGGA GGAGGAGGAG 
TTGGAGGAGG AGGGGGAGGA GGAATTGGAG GAAGAGGATT TGGAGGAGGA GGAGGAGGTG 
CCTGATGACC TGGCCAGCTA CACCCAGCAG GAGGACACCA CTGTGGCTGA GCCCAAAGAG 
TTCAAGCGGA TCAGCTTCCC AACGGCTGCG CCTCAGAAGG ATGACAAGGT TTCAGCTGTG 
CCTACAGAGG CCCCCGAGGT GGCCAAAGGC ATCCCCAAAG CAGCCACACC CGAGGAGAAG 
GCAGCTGCAG AGCGTGCGCA GGAAGCCGAG CCCCCCAAGT CTGAGGAGAG TTTCAGATCT 
CGAGAGGAGG AAGAGGGCCA GGAAGGGCAG GATGCCATGT CCAGAGCCAA AGCCAACTGG 
TTGCGAGCCT TCAACAAGGT GCGCATGCAG CTGCAGGAGG CCCGAGGAGA AGGGGATATG 
TCTAAGTCTC TATGGTTCAA AGGCGGTCCT GGTGGTGGCC TTATCATCAT TGACAGCATG 
CCAGACATCA GGAAACGGAA GCCCATTCCC CTCGTGAGCG ACCTGGCTAT GTCATTGGTG 
CAGTCTCGGA AGGCAGGCAT CACCTCGGCC TTGGCCTCCA GCACGTTGAA CAATGAAGAG 
CTGAAAAATC ACGTTTACAA GAAGACCCTG CAAGCCTTAA TCTACCCCAT CTCCTGCACC 
ACGCCGCACA ATTTCGAGGT GTGGACGGCC ACCACGCCCA CCTACTGCTA TGAGTGCGAG 
GGGCTGCTGT GGGGCATCGC GCGGCAGGGC ATGCGATGCA CCGAGTGCGG CGTTAAGTGC 
CACGAGAAGT GCCAGGACCT GCTCAATGCG GATTGCCTGC AGCGGGCGGC TGAGAAGAGT 
TCCAAGCATG GCGCTGAAGA CCGCACGCAG AACATCATCA TGGTGCTGAA GGACCGCATG 
AAGATCCGCG AGCGCAACAA GCCTGAGATC TTCGAGCTTA TCCAAGAGAT CTTCGCGGTC 
ACCAAGAGCG CACACACACA GCAGATGAAA GCTGTCAAGC AGAGTGTGCT GGACGGCACA 
TCCAAGTGGT CTGCCAAAAT TAGCATCACA GTGGTCTGTG CCCAGGGCTT GCAGGCCAAG 
GACAAGACAG GATCCAGTGA CCCTTATGTC ACCGTCCAGG TTGGGAAGAC CAAGAAAAGG 
ACAAAAACCA TCTACGGGAA CCTCAACCCA GTGTGGGAAG AGAATTTTCA TTTTGAATGT 
CACAACTCCT CTGACCGGAT CAAGGTTCGT GTTTGGGATG AAGATGACGA CATAAAATCC 
CGTGTGAAAC AAAGGTTTAA GAGGGAGTCT GATGACTTCC TAGGGCAGAC AATCATCGAG 
GTGCGGACGC TTAGCGGCGA GATGGATGTG TGGTACAATC TGGACAAGAG AACGGACAAG 
TCGGCGGTGT CGGGGGCCAT CCGGCTTCAC ATCAGTGTGG AGATCAAAGG GGAGGAGAAG 
GTGGCGCCCT ACCATGTCCA GTACACCTGT CTGCATGAGA ACCTGTTCCA CTTTGTGACG 
GACGTGCAGA ACAATGGTGT GGTGAAGATT CCCGATGCCA AGGGTGACGA CGCCTGGAAG 
GTTTACTACG ATGAGACGGC CCAGGAGATC GTGGATGAGT TTGCCATGCG CTATGGTGTT 
GAGTCCATCT ACCAAGCCAT GACCCACTTT GCCTGCCTCT CCTCCAAGTA TATGTGCCCT 
GGGGTACCCG CTGTCATGAG CACCCTGCTT GCCAACATCA ACGCCTACTA CGCACACACC 
ACCGCCTCCA CCAACGTGTC TGCCTCTGAC CGCTTCGCTG CCTCTAATTT CGGGAAAGAG 
CGCTTTGTGA AACTTCTGGA CCAGCTACAC AATTCTCTGC GGATCGACCT GTCCATGTAC 
CGGAACAACT TCCCAGCCAG CAGCCCTGAG CGGCTGCAGG ATCTCAAGTC CACGGTGGAC 
CTGCTCACCA GCATCACCTT CTTCCGGATG AAGGTTCAGG AACTGCAGAG CCCGCCGCGT 
GCCAGCCAGG TAGTGAAAGA CTGTGTGAAG GCCTGCCTCA ACTCCACCTA TGAGTACATC 
TTCAACAACT GCCATGAGCT CTATGGCCGG GAGTACCAAA CGGATCCGGC CAAGAAGGGG 
GAGGTTCCCC CAGAGGAGCA AGGCCCTAGC ATCAAGAACC TGGATTTCTG GTCCAAGCTG 
ATCACCCTCA TCGTGTCTAT CATCGAGGAG GATAAGAATT CTTACACACC CTGCCTCAAT 
CAGTTTCCCC AGGAGCTCAA TGTGGGGAAG ATCAGTGCTG AGGTGATGTG GAGCCTATTT 
GCCCAAGACA TGAAGTACGC CATGGAGGAA CATGACAAAC ACCGGCTGTG TAAGAGCGCA 
GATTACATGA ACCTGCACTT CAAGGTGAAG TGGCTGTACA ATGAGTACGT GGCCGAGCTG 
CCCACCTTCA AGGACCGTGT GCCTGAGTAC CCTGCGTGGT TTGAGCCCTT CGTCATCCAG 
TGGTTGGATG AGAATGAGGA GGTGTCCCGG GACTTCCTGC ATGGTGCCCT CGAGCGGGAC 
AAGAAGGACG GGTTCCAGCA GACCTCAGAG CACGCCCTGT TCTCTTGCTC AGTGGTGGAC 
GTCTTCTCCC AGCTTAACCA GAGTTTCGAG ATCATCAAGA AGCTGGAGTG TCCTGACCCC 
CAGATCGTGG GCCATTACAT GCGGCGTTTT GCCAAGACCA TTAGCAATGT GCTTCTCCAG 
TATGCAGACA TCGTTTCCAA GGACTTCGCC TCCTACTGCT CCAAGGAGAA GGAGAAAGTG 
CCCTGCATCC TCATGAACAA CACACAGCAA CTACGAGTGC AGCTGGAGAA GATGTTTGAG 
GCGATGGGCG GGAAGGAGCT GGATGCCGAG GCCAGTGGCA CCCTGAAGGA GCTGCAGGTG 
AAACTCAACA ATGTCCTGGA TGAACTCAGC CATGTGTTTG CCACCAGCTT CCAGCCACAC 
ATTGAGGAAT GCGTCAGACA GATGGGTGAC ATCCTGAGCC AGGTGAAGGG CACAGGCAAT 
GTACCTGCCA GTGCCTGCAG CAGCGTGGCC CAGGATGCAG ACAATGTGCT GCAGCCCATC 
ATGGATCTTC TGGACAGCAA CCTCACCCTG TTTGCCAAAA TCTGTGAGAA GACAGTTCTG 
AAGCGGGTGC TGAAGGAGCT CTGGAAGCTG GTGATGAACA CAATGGAGAA GACCATCGTC 
CTGCCACCGC TCACTGACCA GACGATGATT GGCACCCTCT TGAGAAAACA TGGCAAGGGC 
CTAGAAAAGG GCAGGGTGAA ACTGCCAAGC CACTCAGACG GGACACAAAT GATCTTCAAT 
GCTGCCAAGG AGCTGGGCCA GCTGTCCAAA CTTAAGGATC ACATGGTGCG GGAAGAAGCC 
AAGAGCTTGA CCCCGAAGCA GTGTGCTGTT GTTGAACTGG CCCTGGACAC CATCAAGCAA 
TACTTCCACG CGGGGGGCGT TGGCCTCAAG AAGACCTTCC TGGAGAAGAG CCCGGACCTC 
CAGTCCCTGC GCTACGCCCT GTCGCTCTAC ACGCAAGCCA CCGACCTGCT CATCAAAACC 
TTCGTGCAGA CGCAGTCAGC GCAGGGCTCT GGTGTGGAGG ACCCTGTAGG TGAAGTGTCC 
GTCCACGTGG AGCTGTTCAC ACATCCAGGA ACTGGGGAAC AGAAGGTCAC AGTGAAGGTG 
GTGGCCGCCA ATGACCTCAA GTGGCAGACT TCTGGCATCT TCCGCCCGTT CATCGAGGTC 
AACATCGTTG GACCTCAGCT TAGCGACAAG AAACGCAAGT TCGCCACCAA ATCCAAAAAC 
AACAGCTGGG CGCCCAAGTA TAACGAGAGC TTCCAGTTCT CCCTGAGCGC CGACGCGGGC 
CCCGAGTGCT ACGAGCTGCA GGTGTGCGTG AAGGACTACT GCTTTGCGCG CGAAGACCGC 
ACGGTGGGGC TGGCGGTGCT GCAACTGCGA GAGCTGGCCC AGCGCGGGAG CGCCGCGTGC 
TGGCTTCCAC TCGGCCGCCG CATCCACATG GACGACACGG GGCTCACAGT GCTGCGCATC 
CTGTCGCAGC GCAGCAACGA TGAGGTGGCC AAGGAATTCG TCAAGCTCAA GTCGGACACG 
CGCTCAGCCG AGGAGGGCGG TGCCGCGCCT GCGCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001025044.2
CDS	111..5249
Translation	MSLLCVGVKKAKFDGAQEKFNTYVTLKVQNVKSTTIAVRGSQPSWEQDFMFEINRLDLGLTVEVWNKGLIWDTMVGTVWIPLRTIRQSNEEGPGEWLTLDSQAIMADSEICGTKDPTFHRILLDAHFELPLDIPEEEARYWAKKLEQLNAMRDQDEYSFQDQQDKPLPVPSSQCCNWNYFGWGEQNDDPDSAVDDRDSDYRSETSNSIPPPYYTTSQPNASVHQYSVRPPPLGSRESYSDSMHSYEEFSEPRALSPTGSSRYASSGELSQGSSQLSEDFDPDEHSLQGSELDDERDRDSYHSCHSSVSYHKDSPRWDQDDEDLEDLEDLEDEELPEEEEELEEEGEEELEEEDLEEEEEVPDDLASYTQQEDTTVAEPKEFKRISFPTAAPQKDDKVSAVPTEAPEVAKGIPKAATPEEKAAAERAQEAEPPKSEESFRSREEEEGQEGQDAMSRAKANWLRAFNKVRMQLQEARGEGDMSKSLWFKGGPGGGLIIIDSMPDIRKRKPIPLVSDLAMSLVQSRKAGITSALASSTLNNEELKNHVYKKTLQALIYPISCTTPHNFEVWTATTPTYCYECEGLLWGIARQGMRCTECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMVLKDRMKIRERNKPEIFELIQEIFAVTKSAHTQQMKAVKQSVLDGTSKWSAKISITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIYGNLNPVWEENFHFECHNSSDRIKVRVWDEDDDIKSRVKQRFKRESDDFLGQTIIEVRTLSGEMDVWYNLDKRTDKSAVSGAIRLHISVEIKGEEKVAPYHVQYTCLHENLFHFVTDVQNNGVVKIPDAKGDDAWKVYYDETAQEIVDEFAMRYGVESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSMYRNNFPASSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHELYGREYQTDPAKKGEVPPEEQGPSIKNLDFWSKLITLIVSIIEEDKNSYTPCLNQFPQELNVGKISAEVMWSLFAQDMKYAMEEHDKHRLCKSADYMNLHFKVKWLYNEYVAELPTFKDRVPEYPAWFEPFVIQWLDENEEVSRDFLHGALERDKKDGFQQTSEHALFSCSVVDVFSQLNQSFEIIKKLECPDPQIVGHYMRRFAKTISNVLLQYADIVSKDFASYCSKEKEKVPCILMNNTQQLRVQLEKMFEAMGGKELDAEASGTLKELQVKLNNVLDELSHVFATSFQPHIEECVRQMGDILSQVKGTGNVPASACSSVAQDADNVLQPIMDLLDSNLTLFAKICEKTVLKRVLKELWKLVMNTMEKTIVLPPLTDQTMIGTLLRKHGKGLEKGRVKLPSHSDGTQMIFNAAKELGQLSKLKDHMVREEAKSLTPKQCAVVELALDTIKQYFHAGGVGLKKTFLEKSPDLQSLRYALSLYTQATDLLIKTFVQTQSAQGSGVEDPVGEVSVHVELFTHPGTGEQKVTVKVVAANDLKWQTSGIFRPFIEVNIVGPQLSDKKRKFATKSKNNSWAPKYNESFQFSLSADAGPECYELQVCVKDYCFAREDRTVGLAVLQLRELAQRGSAACWLPLGRRIHMDDTGLTVLRILSQRSNDEVAKEFVKLKSDTRSAEEGGAAPAP

Target ORF information:

RefSeq Version	NM_001029873.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus unc-13 homolog A (Unc13a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001029873.2

ORF Insert Sequence:

ATGTCTCTGC TGTGCGTGGG AGTCAAAAAA GCCAAGTTTG ACGGTGCCCA AGAGAAGTTC 
AACACATACG TGACGCTGAA GGTGCAGAAC GTGAAGAGCA CTACCATAGC TGTACGTGGC 
AGCCAGCCCA GCTGGGAGCA GGACTTCATG TTTGAGATCA ACCGCCTGGA TCTAGGCCTG 
ACCGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGTAC TGTGTGGATC 
CCGCTGCGGA CCATCCGCCA GTCCAATGAG GAGGGCCCAG GAGAGTGGCT CACACTGGAC 
TCTCAGGCCA TCATGGCGGA CAGTGAGATC TGTGGGACCA AGGACCCCAC CTTCCATCGC 
ATCCTCCTGG ACGCTCATTT CGAGCTGCCG TTGGACATCC CTGAGGAGGA GGCACGCTAC 
TGGGCCAAGA AGCTGGAGCA GCTGAATGCC ATGCGTGACC AGGATGAGTA CTCCTTCCAG 
GACCAGCAGG ACAAGCCGCT GCCAGTGCCC AGCAGCCAGT GCTGCAACTG GAATTACTTT 
GGCTGGGGAG AACAGAATGA CGACCCCGAC AGTGCGGTGG ATGACCGGGA CAGTGACTAT 
AGGAGTGAGA CCAGCAACAG CATCCCACCG CCCTACTATA CGACTTCGCA GCCCAACGCC 
TCTGTGCACC AGTACTCCGT GCGACCGCCC CCTCTGGGGT CCCGGGAGTC CTACAGTGAC 
TCCATGCACA GCTATGAGGA GTTCTCTGAG CCGCGGGCGC TCAGCCCCAC AGGCAGCAGC 
CGCTATGCTT CTTCCGGGGA GCTGAGCCAG GGAAGCTCAC AGCTGAGCGA GGACTTCGAC 
CCTGATGAGC ACAGCCTGCA GGGCTCGGAG CTGGATGACG AGAGGGACCG CGATTCTTAT 
CACTCCTGTC ACAGCTCCGT GAGTTACCAC AAGGACTCAC CCCGCTGGGA CCAGGACGAT 
GAGGACCTGG AGGACCTGGA GGACCTGGAA GATGAGGAGC TGCCCGAGGA GGAGGAGGAG 
TTGGAGGAGG AGGGGGAGGA GGAATTGGAG GAAGAGGATT TGGAGGAGGA GGAGGAGGTG 
CCTGATGACC TGGCCAGCTA CACCCAGCAG GAGGACACCA CTGTGGCTGA GCCCAAAGAG 
TTCAAGCGGA TCAGCTTCCC AACGGCTGCG CCTCAGAAGG ATGACAAGGT TTCAGCTGTG 
CCTACAGAGG CCCCCGAGGT GGCCAAAGGC ATCCCCAAAG CAGCCACACC CGAGGAGAAG 
GCAGCTGCAG AGCGTGCGCA GGAAGCCGAG CCCCCCAAGT CTGAGGAGAG TTTCAGATCT 
CGAGAGGAGG AAGAGGGCCA GGAAGGGCAG GATGCCATGT CCAGAGCCAA AGCCAACTGG 
TTGCGAGCCT TCAACAAGGT GCGCATGCAG CTGCAGGAGG CCCGAGGAGA AGGGGATATG 
TCTAAGTCTC TATGGTTCAA AGGCGGTCCT GGTGGTGGCC TTATCATCAT TGACAGCATG 
CCAGACATCA GGAAACGGAA GCCCATTCCC CTCGTGAGCG ACCTGGCTAT GTCATTGGTG 
CAGTCTCGGA AGGCAGGCAT CACCTCGGCC TTGGCCTCCA GCACGTTGAA CAATGAAGAG 
CTGAAAAATC ACGTTTACAA GAAGACCCTG CAAGCCTTAA TCTACCCCAT CTCCTGCACC 
ACGCCGCACA ATTTCGAGGT GTGGACGGCC ACCACGCCCA CCTACTGCTA TGAGTGCGAG 
GGGCTGCTGT GGGGCATCGC GCGGCAGGGC ATGCGATGCA CCGAGTGCGG CGTTAAGTGC 
CACGAGAAGT GCCAGGACCT GCTCAATGCG GATTGCCTGC AGCGGGCGGC TGAGAAGAGT 
TCCAAGCATG GCGCTGAAGA CCGCACGCAG AACATCATCA TGGTGCTGAA GGACCGCATG 
AAGATCCGCG AGCGCAACAA GCCTGAGATC TTCGAGCTTA TCCAAGAGAT CTTCGCGGTC 
ACCAAGAGCG CACACACACA GCAGATGAAA GCTGTCAAGC AGAGTGTGCT GGACGGCACA 
TCCAAGTGGT CTGCCAAAAT TAGCATCACA GTGGTCTGTG CCCAGGGCTT GCAGGCCAAG 
GACAAGACAG GATCCAGTGA CCCTTATGTC ACCGTCCAGG TTGGGAAGAC CAAGAAAAGG 
ACAAAAACCA TCTACGGGAA CCTCAACCCA GTGTGGGAAG AGAATTTTCA TTTTGAATGT 
CACAACTCCT CTGACCGGAT CAAGGTTCGT GTTTGGGATG AAGATGACGA CATAAAATCC 
CGTGTGAAAC AAAGGTTTAA GAGGGAGTCT GATGACTTCC TAGGGCAGAC AATCATCGAG 
GTGCGGACGC TTAGCGGCGA GATGGATGTG TGGTACAATC TGGACAAGAG AACGGACAAG 
TCGGCGGTGT CGGGGGCCAT CCGGCTTCAC ATCAGTGTGG AGATCAAAGG GGAGGAGAAG 
GTGGCGCCCT ACCATGTCCA GTACACCTGT CTGCATGAGA ACCTGTTCCA CTTTGTGACG 
GACGTGCAGA ACAATGGTGT GGTGAAGATT CCCGATGCCA AGGGTGACGA CGCCTGGAAG 
GTTTACTACG ATGAGACGGC CCAGGAGATC GTGGATGAGT TTGCCATGCG CTATGGTGTT 
GAGTCCATCT ACCAAGCCAT GACCCACTTT GCCTGCCTCT CCTCCAAGTA TATGTGCCCT 
GGGGTACCCG CTGTCATGAG CACCCTGCTT GCCAACATCA ACGCCTACTA CGCACACACC 
ACCGCCTCCA CCAACGTGTC TGCCTCTGAC CGCTTCGCTG CCTCTAATTT CGGGAAAGAG 
CGCTTTGTGA AACTTCTGGA CCAGCTACAC AATTCTCTGC GGATCGACCT GTCCATGTAC 
CGGAACAACT TCCCAGCCAG CAGCCCTGAG CGGCTGCAGG ATCTCAAGTC CACGGTGGAC 
CTGCTCACCA GCATCACCTT CTTCCGGATG AAGGTTCAGG AACTGCAGAG CCCGCCGCGT 
GCCAGCCAGG TAGTGAAAGA CTGTGTGAAG GCCTGCCTCA ACTCCACCTA TGAGTACATC 
TTCAACAACT GCCATGAGCT CTATGGCCGG GAGTACCAAA CGGATCCGGC CAAGAAGGGG 
GAGGTTCCCC CAGAGGAGCA AGGCCCTAGC ATCAAGAACC TGGATTTCTG GTCCAAGCTG 
ATCACCCTCA TCGTGTCTAT CATCGAGGAG GATAAGAATT CTTACACACC CTGCCTCAAT 
CAGTTTCCCC AGGAGCTCAA TGTGGGGAAG ATCAGTGCTG AGGTGATGTG GAGCCTATTT 
GCCCAAGACA TGAAGTACGC CATGGAGGAA CATGACAAAC ACCGGCTGTG TAAGAGCGCA 
GATTACATGA ACCTGCACTT CAAGGTGAAG TGGCTGTACA ATGAGTACGT GGCCGAGCTG 
CCCACCTTCA AGGACCGTGT GCCTGAGTAC CCTGCGTGGT TTGAGCCCTT CGTCATCCAG 
TGGTTGGATG AGAATGAGGA GGTGTCCCGG GACTTCCTGC ATGGTGCCCT CGAGCGGGAC 
AAGAAGGACG GGTTCCAGCA GACCTCAGAG CACGCCCTGT TCTCTTGCTC AGTGGTGGAC 
GTCTTCTCCC AGCTTAACCA GAGTTTCGAG ATCATCAAGA AGCTGGAGTG TCCTGACCCC 
CAGATCGTGG GCCATTACAT GCGGCGTTTT GCCAAGACCA TTAGCAATGT GCTTCTCCAG 
TATGCAGACA TCGTTTCCAA GGACTTCGCC TCCTACTGCT CCAAGGAGAA GGAGAAAGTG 
CCCTGCATCC TCATGAACAA CACACAGCAA CTACGAGTGC AGCTGGAGAA GATGTTTGAG 
GCGATGGGCG GGAAGGAGCT GGATGCCGAG GCCAGTGGCA CCCTGAAGGA GCTGCAGGTG 
AAACTCAACA ATGTCCTGGA TGAACTCAGC CATGTGTTTG CCACCAGCTT CCAGCCACAC 
ATTGAGGAAT GCGTCAGACA GATGGGTGAC ATCCTGAGCC AGGTGAAGGG CACAGGCAAT 
GTACCTGCCA GTGCCTGCAG CAGCGTGGCC CAGGATGCAG ACAATGTGCT GCAGCCCATC 
ATGGATCTTC TGGACAGCAA CCTCACCCTG TTTGCCAAAA TCTGTGAGAA GACAGTTCTG 
AAGCGGGTGC TGAAGGAGCT CTGGAAGCTG GTGATGAACA CAATGGAGAA GACCATCGTC 
CTGCCACCGC TCACTGACCA GACGATGATT GGCACCCTCT TGAGAAAACA TGGCAAGGGC 
CTAGAAAAGG GCAGGGTGAA ACTGCCAAGC CACTCAGACG GGACACAAAT GATCTTCAAT 
GCTGCCAAGG AGCTGGGCCA GCTGTCCAAA CTTAAGGATC ACATGGTGCG GGAAGAAGCC 
AAGAGCTTGA CCCCGAAGCA GTGTGCTGTT GTTGAACTGG CCCTGGACAC CATCAAGCAA 
TACTTCCACG CGGGGGGCGT TGGCCTCAAG AAGACCTTCC TGGAGAAGAG CCCGGACCTC 
CAGTCCCTGC GCTACGCCCT GTCGCTCTAC ACGCAAGCCA CCGACCTGCT CATCAAAACC 
TTCGTGCAGA CGCAGTCAGC GCAGGGCTCT GGTGTGGAGG ACCCTGTAGG TGAAGTGTCC 
GTCCACGTGG AGCTGTTCAC ACATCCAGGA ACTGGGGAAC AGAAGGTCAC AGTGAAGGTG 
GTGGCCGCCA ATGACCTCAA GTGGCAGACT TCTGGCATCT TCCGCCCGTT CATCGAGGTC 
AACATCGTTG GACCTCAGCT TAGCGACAAG AAACGCAAGT TCGCCACCAA ATCCAAAAAC 
AACAGCTGGG CGCCCAAGTA TAACGAGAGC TTCCAGTTCT CCCTGAGCGC CGACGCGGGC 
CCCGAGTGCT ACGAGCTGCA GGTGTGCGTG AAGGACTACT GCTTTGCGCG CGAAGACCGC 
ACGGTGGGGC TGGCGGTGCT GCAACTGCGA GAGCTGGCCC AGCGCGGGAG CGCCGCGTGC 
TGGCTTCCAC TCGGCCGCCG CATCCACATG GACGACACGG GGCTCACAGT GCTGCGCATC 
CTGTCGCAGC GCAGCAACGA TGAGGTGGCC AAGGAATTCG TCAAGCTCAA GTCGGACACG 
CGCTCAGCCG AGGAGGGCGG TGCCGCGCCT GCGCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Unc13a ( XM_006509692.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006509755.1 Mus musculus unc-13 homolog A (C. elegans) (Unc13a), transcript variant X3, mRNA.

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CloneID	OMu41018
Clone ID Related Accession (Same CDS sequence)	XM_006509692.3 , XM_006509692.4
Accession Version	XM_006509692.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5196bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein unc-13 homolog A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006509692.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTGC TGTGCGTGGG AGTCAAAAAA GCCAAGTTTG ACGGTGCCCA AGAGAAGTTC 
AACACATACG TGACGCTGAA GGTGCAGAAC GTGAAGAGCA CTACCATAGC TGTACGTGGC 
AGCCAGCCCA GCTGGGAGCA GGACTTCATG TTTGAGATCA ACCGCCTGGA TCTAGGCCTG 
ACCGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGTAC TGTGTGGATC 
CCGCTGCGGA CCATCCGCCA GTCCAATGAG GAGGGCCCAG GAGAGTGGCT CACACTGGAC 
TCTCAGGCCA TCATGGCGGA CAGTGAGATC TGTGGGACCA AGGACCCCAC CTTCCATCGC 
ATCCTCCTGG ACGCTCATTT CGAGCTGCCG TTGGACATCC CTGAGGAGGA GGCACGCTAC 
TGGGCCAAGA AGCTGGAGCA GCTGAATGCC ATGCGTGACC AGGATGAGTA CTCCTTCCAG 
GACCAGCAGG ACAAGCCGCT GCCAGTGCCC AGCAGCCAGT GCTGCAACTG GAATTACTTT 
GGCTGGGGAG AACAGAATGA CGACCCCGAC AGTGCGGTGG ATGACCGGGA CAGTGACTAT 
AGGAGTGAGA CCAGCAACAG CATCCCACCG CCCTACTATA CGACTTCGCA GCCCAACGCC 
TCTGTGCACC AGTACTCCGT GCGACCGCCC CCTCTGGGGT CCCGGGAGTC CTACAGTGAC 
TCCATGCACA GCTATGAGGA GTTCTCTGAG CCGCGGGCGC TCAGCCCCAC AGGCAGCAGC 
CGCTATGCTT CTTCCGGGGA GCTGAGCCAG GGAAGCTCAC AGCTGAGCGA GGACTTCGAC 
CCTGATGAGC ACAGCCTGCA GGGCTCGGAG CTGGATGACG AGAGGGACCG CGATTCTTAT 
CACTCCTGTC ACAGCTCCGT GAGTTACCAC AAGGACTCAC CCCGCTGGGA CCAGGACGAT 
GAGGACCTGG AGGACCTGGA GGACCTGGAA GATGAGGAGC TGCCCGAGGA GGAGGAGGAG 
TTGGAGGAGG AGGGGGAGGA GGAATTGGAG GAAGAGGATT TGGAGGAGGA GGAGGAGGTG 
CCTGATGACC TGGCCAGCTA CACCCAGCAG GAGGACACCA CTGTGGCTGA GCCCAAAGAG 
TTCAAGCGGA TCAGCTTCCC AACGGCTGCG CCTCAGAAGG ATGACAAGGT TTCAGCTGTG 
CCTACAGAGG CCCCCGAGGT GGCCAAAGGC ATCCCCAAAG CAGCCACACC CGAGGAGAAG 
GCAGCTGCAG AGCGTGCGCA GGAAGCCGAG CCCCCCAAGT CTGAGGAGAG TTTCAGATCT 
CGAGAGGAGG AAGAGGGCCA GGAAGGGCAG GATGCCATGT CCAGAGCCAA AGCCAACTGG 
TTGCGAGCCT TCAACAAGGT GCGCATGCAG CTGCAGGAGG CCCGAGGAGA AGGGGATATG 
TCTAAGTCTC TATGGTTCAA AGGCGGTCCT GGTGGTGGCC TTATCATCAT TGACAGCATG 
CCAGACATCA GGAAACGGAA GCCCATTCCC CTCGTGAGCG ACCTGGCTAT GTCATTGGTG 
CAGTCTCGGA AGGCAGGCAT CACCTCGGCC TTGGCCTCCA GCACGTTGAA CAATGAAGAG 
CTGAAAAATC ACGTTTACAA GAAGACCCTG CAAGCCTTAA TCTACCCCAT CTCCTGCACC 
ACGCCGCACA ATTTCGAGGT GTGGACGGCC ACCACGCCCA CCTACTGCTA TGAGTGCGAG 
GGGCTGCTGT GGGGCATCGC GCGGCAGGGC ATGCGATGCA CCGAGTGCGG CGTTAAGTGC 
CACGAGAAGT GCCAGGACCT GCTCAATGCG GATTGCCTGC AGCGGGCGGC TGAGAAGAGT 
TCCAAGCATG GCGCTGAAGA CCGCACGCAG AACATCATCA TGGTGCTGAA GGACCGCATG 
AAGATCCGCG AGCGCAACAA GCCTGAGATC TTCGAGCTTA TCCAAGAGAT CTTCGCGGTC 
ACCAAGAGCG CACACACACA GCAGATGAAA GCTGTCAAGC AGAGTGTGCT GGACGGCACA 
TCCAAGTGGT CTGCCAAAAT TAGCATCACA GTGGTCTGTG CCCAGGGCTT GCAGGCCAAG 
GACAAGACAG GATCCAGTGA CCCTTATGTC ACCGTCCAGG TTGGGAAGAC CAAGAAAAGG 
ACAAAAACCA TCTACGGGAA CCTCAACCCA GTGTGGGAAG AGAATTTTCA TTTTGAATGT 
CACAACTCCT CTGACCGGAT CAAGGTTCGT GTTTGGGATG AAGATGACGA CATAAAATCC 
CGTGTGAAAC AAAGGTTTAA GAGGGAGTCT GATGACTTCC TAGGGCAGAC AATCATCGAG 
GTGCGGACGC TTAGCGGCGA GATGGATGTG TGGTACAATC TGGACAAGAG AACGGACAAG 
TCGGCGGTGT CGGGGGCCAT CCGGCTTCAC ATCAGTGTGG AGATCAAAGG GGAGGAGAAG 
GTGGCGCCCT ACCATGTCCA GTACACCTGT CTGCATGAGA ACCTGTTCCA CTTTGTGACG 
GACGTGCAGA ACAATGGTGT GGTGAAGATT CCCGATGCCA AGGGTGACGA CGCCTGGAAG 
GTTTACTACG ATGAGACGGC CCAGGAGATC GTGGATGAGT TTGCCATGCG CTATGGTGTT 
GAGTCCATCT ACCAAGCCAT GACCCACTTT GCCTGCCTCT CCTCCAAGTA TATGTGCCCT 
GGGGTACCCG CTGTCATGAG CACCCTGCTT GCCAACATCA ACGCCTACTA CGCACACACC 
ACCGCCTCCA CCAACGTGTC TGCCTCTGAC CGCTTCGCTG CCTCTAATTT CGGGAAAGAG 
CGCTTTGTGA AACTTCTGGA CCAGCTACAC AATTCTCTGC GGATCGACCT GTCCATGTAC 
CGGAACAACT TCCCAGCCAG CAGCCCTGAG CGGCTGCAGG ATCTCAAGTC CACGGTGGAC 
CTGCTCACCA GCATCACCTT CTTCCGGATG AAGGTTCAGG AACTGCAGAG CCCGCCGCGT 
GCCAGCCAGG TAGTGAAAGA CTGTGTGAAG GCCTGCCTCA ACTCCACCTA TGAGTACATC 
TTCAACAACT GCCATGAGCT CTATGGCCGG GAGTACCAAA CGGATCCGGC CAAGAAGGGG 
GAGGTTCCCC CAGAGGAGCA AGGCCCTAGC ATCAAGAACC TGGATTTCTG GTCCAAGCTG 
ATCACCCTCA TCGTGTCTAT CATCGAGGAG GATAAGAATT CTTACACACC CTGCCTCAAT 
CAGTTTCCCC AGGAGCTCAA TGTGGGGAAG ATCAGTGCTG AGGTGATGTG GAGCCTATTT 
GCCCAAGACA TGAAGTACGC CATGGAGGAA CATGACAAAC ACCGGCTGTG TAAGAGCGCA 
GATTACATGA ACCTGCACTT CAAGGTGAAG TGGCTGTACA ATGAGTACGT GGCCGAGCTG 
CCCACCTTCA AGGACCGTGT GCCTGAGTAC CCTGCGTGGT TTGAGCCCTT CGTCATCCAG 
TGGTTGGATG AGAATGAGGA GGTGTCCCGG GACTTCCTGC ATGGTGCCCT CGAGCGGGAC 
AAGAAGGACG GGTTCCAGCA GACCTCAGAG CACGCCCTGT TCTCTTGCTC AGTGGTGGAC 
GTCTTCTCCC AGCTTAACCA GAGTTTCGAG ATCATCAAGA AGCTGGAGTG TCCTGACCCC 
CAGATCGTGG GCCATTACAT GCGGCGTTTT GCCAAGACCA TTAGCAATGT GCTTCTCCAG 
TATGCAGACA TCGTTTCCAA GGACTTCGCC TCCTACTGCT CCAAGGAGAA GGAGAAAGTG 
CCCTGCATCC TCATGAACAA CACACAGCAA CTACGAGTGC AGCTGGAGAA GATGTTTGAG 
GCGATGGGCG GGAAGGAGCT GGATGCCGAG GCCAGTGGCA CCCTGAAGGA GCTGCAGGTG 
AAACTCAACA ATGTCCTGGA TGAACTCAGC CATGTGTTTG CCACCAGCTT CCAGCCACAC 
ATTGAGGAAT GCGTCAGACA GATGGGTGAC ATCCTGAGCC AGGTGAAGGG CACAGGCAAT 
GTACCTGCCA GTGCCTGCAG CAGCGTGGCC CAGGATGCAG ACAATGTGCT GCAGCCCATC 
ATGGATCTTC TGGACAGCAA CCTCACCCTG TTTGCCAAAA TCTGTGAGAA GACAGTTCTG 
AAGCGGGTGC TGAAGGAGCT CTGGAAGCTG GTGATGAACA CAATGGAGAA GACCATCGTC 
CTGCCACCGC TCACTGACCA GACGATGATT GGCACCCTCT TGAGAAAACA TGGCAAGGGC 
CTAGAAAAGG GCAGGGTGAA ACTGCCAAGC CACTCAGACG GGACACAAAT GATCTTCAAT 
GCTGCCAAGG AGCTGGGCCA GCTGTCCAAA CTTAAGGATC ACATGGTGCG GGAAGAAGCC 
AAGAGCTTGA CCCCGAAGCA GTGTGCTGTT GTTGAACTGG CCCTGGACAC CATCAAGCAA 
TACTTCCACG CGGGGGGCGT TGGCCTCAAG AAGACCTTCC TGGAGAAGAG CCCGGACCTC 
CAGTCCCTGC GCTACGCCCT GTCGCTCTAC ACGCAAGCCA CCGACCTGCT CATCAAAACC 
TTCGTGCAGA CGCAGTCAGC GCAGGTCCAT GGTGGAAAAG GGACTAGGTT TACCCTTAGT 
GAAGACGTTT GTCCTGAGAT GGGCTCTGGT GTGGAGGACC CTGTAGGTGA AGTGTCCGTC 
CACGTGGAGC TGTTCACACA TCCAGGAACT GGGGAACAGA AGGTCACAGT GAAGGTGGTG 
GCCGCCAATG ACCTCAAGTG GCAGACTTCT GGCATCTTCC GCCCGTTCAT CGAGGTCAAC 
ATCGTTGGAC CTCAGCTTAG CGACAAGAAA CGCAAGTTCG CCACCAAATC CAAAAACAAC 
AGCTGGGCGC CCAAGTATAA CGAGAGCTTC CAGTTCTCCC TGAGCGCCGA CGCGGGCCCC 
GAGTGCTACG AGCTGCAGGT GTGCGTGAAG GACTACTGCT TTGCGCGCGA AGACCGCACG 
GTGGGGCTGG CGGTGCTGCA ACTGCGAGAG CTGGCCCAGC GCGGGAGCGC CGCGTGCTGG 
CTTCCACTCG GCCGCCGCAT CCACATGGAC GACACGGGGC TCACAGTGCT GCGCATCCTG 
TCGCAGCGCA GCAACGATGA GGTGGCCAAG GAATTCGTCA AGCTCAAGTC GGACACGCGC 
TCAGCCGAGG AGGGCGGTGC CGCGCCTGCG CCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006509755.1
CDS	125..5320
Translation	MSLLCVGVKKAKFDGAQEKFNTYVTLKVQNVKSTTIAVRGSQPSWEQDFMFEINRLDLGLTVEVWNKGLIWDTMVGTVWIPLRTIRQSNEEGPGEWLTLDSQAIMADSEICGTKDPTFHRILLDAHFELPLDIPEEEARYWAKKLEQLNAMRDQDEYSFQDQQDKPLPVPSSQCCNWNYFGWGEQNDDPDSAVDDRDSDYRSETSNSIPPPYYTTSQPNASVHQYSVRPPPLGSRESYSDSMHSYEEFSEPRALSPTGSSRYASSGELSQGSSQLSEDFDPDEHSLQGSELDDERDRDSYHSCHSSVSYHKDSPRWDQDDEDLEDLEDLEDEELPEEEEELEEEGEEELEEEDLEEEEEVPDDLASYTQQEDTTVAEPKEFKRISFPTAAPQKDDKVSAVPTEAPEVAKGIPKAATPEEKAAAERAQEAEPPKSEESFRSREEEEGQEGQDAMSRAKANWLRAFNKVRMQLQEARGEGDMSKSLWFKGGPGGGLIIIDSMPDIRKRKPIPLVSDLAMSLVQSRKAGITSALASSTLNNEELKNHVYKKTLQALIYPISCTTPHNFEVWTATTPTYCYECEGLLWGIARQGMRCTECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMVLKDRMKIRERNKPEIFELIQEIFAVTKSAHTQQMKAVKQSVLDGTSKWSAKISITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIYGNLNPVWEENFHFECHNSSDRIKVRVWDEDDDIKSRVKQRFKRESDDFLGQTIIEVRTLSGEMDVWYNLDKRTDKSAVSGAIRLHISVEIKGEEKVAPYHVQYTCLHENLFHFVTDVQNNGVVKIPDAKGDDAWKVYYDETAQEIVDEFAMRYGVESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSMYRNNFPASSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHELYGREYQTDPAKKGEVPPEEQGPSIKNLDFWSKLITLIVSIIEEDKNSYTPCLNQFPQELNVGKISAEVMWSLFAQDMKYAMEEHDKHRLCKSADYMNLHFKVKWLYNEYVAELPTFKDRVPEYPAWFEPFVIQWLDENEEVSRDFLHGALERDKKDGFQQTSEHALFSCSVVDVFSQLNQSFEIIKKLECPDPQIVGHYMRRFAKTISNVLLQYADIVSKDFASYCSKEKEKVPCILMNNTQQLRVQLEKMFEAMGGKELDAEASGTLKELQVKLNNVLDELSHVFATSFQPHIEECVRQMGDILSQVKGTGNVPASACSSVAQDADNVLQPIMDLLDSNLTLFAKICEKTVLKRVLKELWKLVMNTMEKTIVLPPLTDQTMIGTLLRKHGKGLEKGRVKLPSHSDGTQMIFNAAKELGQLSKLKDHMVREEAKSLTPKQCAVVELALDTIKQYFHAGGVGLKKTFLEKSPDLQSLRYALSLYTQATDLLIKTFVQTQSAQVHGGKGTRFTLSEDVCPEMGSGVEDPVGEVSVHVELFTHPGTGEQKVTVKVVAANDLKWQTSGIFRPFIEVNIVGPQLSDKKRKFATKSKNNSWAPKYNESFQFSLSADAGPECYELQVCVKDYCFAREDRTVGLAVLQLRELAQRGSAACWLPLGRRIHMDDTGLTVLRILSQRSNDEVAKEFVKLKSDTRSAEEGGAAPAP

Target ORF information:

RefSeq Version	XM_006509692.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus unc-13 homolog A (C. elegans) (Unc13a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006509692.3

ORF Insert Sequence:

ATGTCTCTGC TGTGCGTGGG AGTCAAAAAA GCCAAGTTTG ACGGTGCCCA AGAGAAGTTC 
AACACATACG TGACGCTGAA GGTGCAGAAC GTGAAGAGCA CTACCATAGC TGTACGTGGC 
AGCCAGCCCA GCTGGGAGCA GGACTTCATG TTTGAGATCA ACCGCCTGGA TCTAGGCCTG 
ACCGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGTAC TGTGTGGATC 
CCGCTGCGGA CCATCCGCCA GTCCAATGAG GAGGGCCCAG GAGAGTGGCT CACACTGGAC 
TCTCAGGCCA TCATGGCGGA CAGTGAGATC TGTGGGACCA AGGACCCCAC CTTCCATCGC 
ATCCTCCTGG ACGCTCATTT CGAGCTGCCG TTGGACATCC CTGAGGAGGA GGCACGCTAC 
TGGGCCAAGA AGCTGGAGCA GCTGAATGCC ATGCGTGACC AGGATGAGTA CTCCTTCCAG 
GACCAGCAGG ACAAGCCGCT GCCAGTGCCC AGCAGCCAGT GCTGCAACTG GAATTACTTT 
GGCTGGGGAG AACAGAATGA CGACCCCGAC AGTGCGGTGG ATGACCGGGA CAGTGACTAT 
AGGAGTGAGA CCAGCAACAG CATCCCACCG CCCTACTATA CGACTTCGCA GCCCAACGCC 
TCTGTGCACC AGTACTCCGT GCGACCGCCC CCTCTGGGGT CCCGGGAGTC CTACAGTGAC 
TCCATGCACA GCTATGAGGA GTTCTCTGAG CCGCGGGCGC TCAGCCCCAC AGGCAGCAGC 
CGCTATGCTT CTTCCGGGGA GCTGAGCCAG GGAAGCTCAC AGCTGAGCGA GGACTTCGAC 
CCTGATGAGC ACAGCCTGCA GGGCTCGGAG CTGGATGACG AGAGGGACCG CGATTCTTAT 
CACTCCTGTC ACAGCTCCGT GAGTTACCAC AAGGACTCAC CCCGCTGGGA CCAGGACGAT 
GAGGACCTGG AGGACCTGGA GGACCTGGAA GATGAGGAGC TGCCCGAGGA GGAGGAGGAG 
TTGGAGGAGG AGGGGGAGGA GGAATTGGAG GAAGAGGATT TGGAGGAGGA GGAGGAGGTG 
CCTGATGACC TGGCCAGCTA CACCCAGCAG GAGGACACCA CTGTGGCTGA GCCCAAAGAG 
TTCAAGCGGA TCAGCTTCCC AACGGCTGCG CCTCAGAAGG ATGACAAGGT TTCAGCTGTG 
CCTACAGAGG CCCCCGAGGT GGCCAAAGGC ATCCCCAAAG CAGCCACACC CGAGGAGAAG 
GCAGCTGCAG AGCGTGCGCA GGAAGCCGAG CCCCCCAAGT CTGAGGAGAG TTTCAGATCT 
CGAGAGGAGG AAGAGGGCCA GGAAGGGCAG GATGCCATGT CCAGAGCCAA AGCCAACTGG 
TTGCGAGCCT TCAACAAGGT GCGCATGCAG CTGCAGGAGG CCCGAGGAGA AGGGGATATG 
TCTAAGTCTC TATGGTTCAA AGGCGGTCCT GGTGGTGGCC TTATCATCAT TGACAGCATG 
CCAGACATCA GGAAACGGAA GCCCATTCCC CTCGTGAGCG ACCTGGCTAT GTCATTGGTG 
CAGTCTCGGA AGGCAGGCAT CACCTCGGCC TTGGCCTCCA GCACGTTGAA CAATGAAGAG 
CTGAAAAATC ACGTTTACAA GAAGACCCTG CAAGCCTTAA TCTACCCCAT CTCCTGCACC 
ACGCCGCACA ATTTCGAGGT GTGGACGGCC ACCACGCCCA CCTACTGCTA TGAGTGCGAG 
GGGCTGCTGT GGGGCATCGC GCGGCAGGGC ATGCGATGCA CCGAGTGCGG CGTTAAGTGC 
CACGAGAAGT GCCAGGACCT GCTCAATGCG GATTGCCTGC AGCGGGCGGC TGAGAAGAGT 
TCCAAGCATG GCGCTGAAGA CCGCACGCAG AACATCATCA TGGTGCTGAA GGACCGCATG 
AAGATCCGCG AGCGCAACAA GCCTGAGATC TTCGAGCTTA TCCAAGAGAT CTTCGCGGTC 
ACCAAGAGCG CACACACACA GCAGATGAAA GCTGTCAAGC AGAGTGTGCT GGACGGCACA 
TCCAAGTGGT CTGCCAAAAT TAGCATCACA GTGGTCTGTG CCCAGGGCTT GCAGGCCAAG 
GACAAGACAG GATCCAGTGA CCCTTATGTC ACCGTCCAGG TTGGGAAGAC CAAGAAAAGG 
ACAAAAACCA TCTACGGGAA CCTCAACCCA GTGTGGGAAG AGAATTTTCA TTTTGAATGT 
CACAACTCCT CTGACCGGAT CAAGGTTCGT GTTTGGGATG AAGATGACGA CATAAAATCC 
CGTGTGAAAC AAAGGTTTAA GAGGGAGTCT GATGACTTCC TAGGGCAGAC AATCATCGAG 
GTGCGGACGC TTAGCGGCGA GATGGATGTG TGGTACAATC TGGACAAGAG AACGGACAAG 
TCGGCGGTGT CGGGGGCCAT CCGGCTTCAC ATCAGTGTGG AGATCAAAGG GGAGGAGAAG 
GTGGCGCCCT ACCATGTCCA GTACACCTGT CTGCATGAGA ACCTGTTCCA CTTTGTGACG 
GACGTGCAGA ACAATGGTGT GGTGAAGATT CCCGATGCCA AGGGTGACGA CGCCTGGAAG 
GTTTACTACG ATGAGACGGC CCAGGAGATC GTGGATGAGT TTGCCATGCG CTATGGTGTT 
GAGTCCATCT ACCAAGCCAT GACCCACTTT GCCTGCCTCT CCTCCAAGTA TATGTGCCCT 
GGGGTACCCG CTGTCATGAG CACCCTGCTT GCCAACATCA ACGCCTACTA CGCACACACC 
ACCGCCTCCA CCAACGTGTC TGCCTCTGAC CGCTTCGCTG CCTCTAATTT CGGGAAAGAG 
CGCTTTGTGA AACTTCTGGA CCAGCTACAC AATTCTCTGC GGATCGACCT GTCCATGTAC 
CGGAACAACT TCCCAGCCAG CAGCCCTGAG CGGCTGCAGG ATCTCAAGTC CACGGTGGAC 
CTGCTCACCA GCATCACCTT CTTCCGGATG AAGGTTCAGG AACTGCAGAG CCCGCCGCGT 
GCCAGCCAGG TAGTGAAAGA CTGTGTGAAG GCCTGCCTCA ACTCCACCTA TGAGTACATC 
TTCAACAACT GCCATGAGCT CTATGGCCGG GAGTACCAAA CGGATCCGGC CAAGAAGGGG 
GAGGTTCCCC CAGAGGAGCA AGGCCCTAGC ATCAAGAACC TGGATTTCTG GTCCAAGCTG 
ATCACCCTCA TCGTGTCTAT CATCGAGGAG GATAAGAATT CTTACACACC CTGCCTCAAT 
CAGTTTCCCC AGGAGCTCAA TGTGGGGAAG ATCAGTGCTG AGGTGATGTG GAGCCTATTT 
GCCCAAGACA TGAAGTACGC CATGGAGGAA CATGACAAAC ACCGGCTGTG TAAGAGCGCA 
GATTACATGA ACCTGCACTT CAAGGTGAAG TGGCTGTACA ATGAGTACGT GGCCGAGCTG 
CCCACCTTCA AGGACCGTGT GCCTGAGTAC CCTGCGTGGT TTGAGCCCTT CGTCATCCAG 
TGGTTGGATG AGAATGAGGA GGTGTCCCGG GACTTCCTGC ATGGTGCCCT CGAGCGGGAC 
AAGAAGGACG GGTTCCAGCA GACCTCAGAG CACGCCCTGT TCTCTTGCTC AGTGGTGGAC 
GTCTTCTCCC AGCTTAACCA GAGTTTCGAG ATCATCAAGA AGCTGGAGTG TCCTGACCCC 
CAGATCGTGG GCCATTACAT GCGGCGTTTT GCCAAGACCA TTAGCAATGT GCTTCTCCAG 
TATGCAGACA TCGTTTCCAA GGACTTCGCC TCCTACTGCT CCAAGGAGAA GGAGAAAGTG 
CCCTGCATCC TCATGAACAA CACACAGCAA CTACGAGTGC AGCTGGAGAA GATGTTTGAG 
GCGATGGGCG GGAAGGAGCT GGATGCCGAG GCCAGTGGCA CCCTGAAGGA GCTGCAGGTG 
AAACTCAACA ATGTCCTGGA TGAACTCAGC CATGTGTTTG CCACCAGCTT CCAGCCACAC 
ATTGAGGAAT GCGTCAGACA GATGGGTGAC ATCCTGAGCC AGGTGAAGGG CACAGGCAAT 
GTACCTGCCA GTGCCTGCAG CAGCGTGGCC CAGGATGCAG ACAATGTGCT GCAGCCCATC 
ATGGATCTTC TGGACAGCAA CCTCACCCTG TTTGCCAAAA TCTGTGAGAA GACAGTTCTG 
AAGCGGGTGC TGAAGGAGCT CTGGAAGCTG GTGATGAACA CAATGGAGAA GACCATCGTC 
CTGCCACCGC TCACTGACCA GACGATGATT GGCACCCTCT TGAGAAAACA TGGCAAGGGC 
CTAGAAAAGG GCAGGGTGAA ACTGCCAAGC CACTCAGACG GGACACAAAT GATCTTCAAT 
GCTGCCAAGG AGCTGGGCCA GCTGTCCAAA CTTAAGGATC ACATGGTGCG GGAAGAAGCC 
AAGAGCTTGA CCCCGAAGCA GTGTGCTGTT GTTGAACTGG CCCTGGACAC CATCAAGCAA 
TACTTCCACG CGGGGGGCGT TGGCCTCAAG AAGACCTTCC TGGAGAAGAG CCCGGACCTC 
CAGTCCCTGC GCTACGCCCT GTCGCTCTAC ACGCAAGCCA CCGACCTGCT CATCAAAACC 
TTCGTGCAGA CGCAGTCAGC GCAGGTCCAT GGTGGAAAAG GGACTAGGTT TACCCTTAGT 
GAAGACGTTT GTCCTGAGAT GGGCTCTGGT GTGGAGGACC CTGTAGGTGA AGTGTCCGTC 
CACGTGGAGC TGTTCACACA TCCAGGAACT GGGGAACAGA AGGTCACAGT GAAGGTGGTG 
GCCGCCAATG ACCTCAAGTG GCAGACTTCT GGCATCTTCC GCCCGTTCAT CGAGGTCAAC 
ATCGTTGGAC CTCAGCTTAG CGACAAGAAA CGCAAGTTCG CCACCAAATC CAAAAACAAC 
AGCTGGGCGC CCAAGTATAA CGAGAGCTTC CAGTTCTCCC TGAGCGCCGA CGCGGGCCCC 
GAGTGCTACG AGCTGCAGGT GTGCGTGAAG GACTACTGCT TTGCGCGCGA AGACCGCACG 
GTGGGGCTGG CGGTGCTGCA ACTGCGAGAG CTGGCCCAGC GCGGGAGCGC CGCGTGCTGG 
CTTCCACTCG GCCGCCGCAT CCACATGGAC GACACGGGGC TCACAGTGCT GCGCATCCTG 
TCGCAGCGCA GCAACGATGA GGTGGCCAAG GAATTCGTCA AGCTCAAGTC GGACACGCGC 
TCAGCCGAGG AGGGCGGTGC CGCGCCTGCG CCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Unc13a ( XM_006509693.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006509756.1 Mus musculus unc-13 homolog A (C. elegans) (Unc13a), transcript variant X4, mRNA.

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CloneID	OMu41019
Clone ID Related Accession (Same CDS sequence)	XM_006509693.4 , XM_006509693.3
Accession Version	XM_006509693.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5181bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein unc-13 homolog A isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006509693.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTGC TGTGCGTGGG AGAGAAGTTC AACACATACG TGACGCTGAA GGTGCAGAAC 
GTGAAGAGCA CTACCATAGC TGTACGTGGC AGCCAGCCCA GCTGGGAGCA GGACTTCATG 
TTTGAGATCA ACCGCCTGGA TCTAGGCCTG ACCGTGGAGG TGTGGAACAA GGGTCTCATC 
TGGGACACGA TGGTGGGTAC TGTGTGGATC CCGCTGCGGA CCATCCGCCA GTCCAATGAG 
GAGGGCCCAG GAGAGTGGCT CACACTGGAC TCTCAGGCCA TCATGGCGGA CAGTGAGATC 
TGTGGGACCA AGGACCCCAC CTTCCATCGC ATCCTCCTGG ACGCTCATTT CGAGCTGCCG 
TTGGACATCC CTGAGGAGGA GGCACGCTAC TGGGCCAAGA AGCTGGAGCA GCTGAATGCC 
ATGCGTGACC AGGATGAGGT GAGTCACCTC CAGTACTCCT TCCAGGACCA GCAGGACAAG 
CCGCTGCCAG TGCCCAGCAG CCAGTGCTGC AACTGGAATT ACTTTGGCTG GGGAGAACAG 
AATGACGACC CCGACAGTGC GGTGGATGAC CGGGACAGTG ACTATAGGAG TGAGACCAGC 
AACAGCATCC CACCGCCCTA CTATACGACT TCGCAGCCCA ACGCCTCTGT GCACCAGTAC 
TCCGTGCGAC CGCCCCCTCT GGGGTCCCGG GAGTCCTACA GTGACTCCAT GCACAGCTAT 
GAGGAGTTCT CTGAGCCGCG GGCGCTCAGC CCCACAGGCA GCAGCCGCTA TGCTTCTTCC 
GGGGAGCTGA GCCAGGGAAG CTCACAGCTG AGCGAGGACT TCGACCCTGA TGAGCACAGC 
CTGCAGGGCT CGGAGCTGGA TGACGAGAGG GACCGCGATT CTTATCACTC CTGTCACAGC 
TCCGTGAGTT ACCACAAGGA CTCACCCCGC TGGGACCAGG ACGATGAGGA CCTGGAGGAC 
CTGGAGGACC TGGAAGATGA GGAGCTGCCC GAGGAGGAGG AGGAGTTGGA GGAGGAGGGG 
GAGGAGGAAT TGGAGGAAGA GGATTTGGAG GAGGAGGAGG AGGTGCCTGA TGACCTGGCC 
AGCTACACCC AGCAGGAGGA CACCACTGTG GCTGAGCCCA AAGAGTTCAA GCGGATCAGC 
TTCCCAACGG CTGCGCCTCA GAAGGATGAC AAGGTTTCAG CTGTGCCTAC AGAGGCCCCC 
GAGGTGGCCA AAGGCATCCC CAAAGCAGCC ACACCCGAGG AGAAGGCAGC TGCAGAGCGT 
GCGCAGGAAG CCGAGCCCCC CAAGTCTGAG GAGAGTTTCA GATCTCGAGA GGAGGAAGAG 
GGCCAGGAAG GGCAGGATGC CATGTCCAGA GCCAAAGCCA ACTGGTTGCG AGCCTTCAAC 
AAGGTGCGCA TGCAGCTGCA GGAGGCCCGA GGAGAAGGGG ATATGTCTAA GTCTCTATGG 
TTCAAAGGCG GTCCTGGTGG TGGCCTTATC ATCATTGACA GCATGCCAGA CATCAGGAAA 
CGGAAGCCCA TTCCCCTCGT GAGCGACCTG GCTATGTCAT TGGTGCAGTC TCGGAAGGCA 
GGCATCACCT CGGCCTTGGC CTCCAGCACG TTGAACAATG AAGAGCTGAA AAATCACGTT 
TACAAGAAGA CCCTGCAAGC CTTAATCTAC CCCATCTCCT GCACCACGCC GCACAATTTC 
GAGGTGTGGA CGGCCACCAC GCCCACCTAC TGCTATGAGT GCGAGGGGCT GCTGTGGGGC 
ATCGCGCGGC AGGGCATGCG ATGCACCGAG TGCGGCGTTA AGTGCCACGA GAAGTGCCAG 
GACCTGCTCA ATGCGGATTG CCTGCAGCGG GCGGCTGAGA AGAGTTCCAA GCATGGCGCT 
GAAGACCGCA CGCAGAACAT CATCATGGTG CTGAAGGACC GCATGAAGAT CCGCGAGCGC 
AACAAGCCTG AGATCTTCGA GCTTATCCAA GAGATCTTCG CGGTCACCAA GAGCGCACAC 
ACACAGCAGA TGAAAGCTGT CAAGCAGAGT GTGCTGGACG GCACATCCAA GTGGTCTGCC 
AAAATTAGCA TCACAGTGGT CTGTGCCCAG GGCTTGCAGG CCAAGGACAA GACAGGATCC 
AGTGACCCTT ATGTCACCGT CCAGGTTGGG AAGACCAAGA AAAGGACAAA AACCATCTAC 
GGGAACCTCA ACCCAGTGTG GGAAGAGAAT TTTCATTTTG AATGTCACAA CTCCTCTGAC 
CGGATCAAGG TTCGTGTTTG GGATGAAGAT GACGACATAA AATCCCGTGT GAAACAAAGG 
TTTAAGAGGG AGTCTGATGA CTTCCTAGGG CAGACAATCA TCGAGGTGCG GACGCTTAGC 
GGCGAGATGG ATGTGTGGTA CAATCTGGAC AAGAGAACGG ACAAGTCGGC GGTGTCGGGG 
GCCATCCGGC TTCACATCAG TGTGGAGATC AAAGGGGAGG AGAAGGTGGC GCCCTACCAT 
GTCCAGTACA CCTGTCTGCA TGAGAACCTG TTCCACTTTG TGACGGACGT GCAGAACAAT 
GGTGTGGTGA AGATTCCCGA TGCCAAGGGT GACGACGCCT GGAAGGTTTA CTACGATGAG 
ACGGCCCAGG AGATCGTGGA TGAGTTTGCC ATGCGCTATG GTGTTGAGTC CATCTACCAA 
GCCATGACCC ACTTTGCCTG CCTCTCCTCC AAGTATATGT GCCCTGGGGT ACCCGCTGTC 
ATGAGCACCC TGCTTGCCAA CATCAACGCC TACTACGCAC ACACCACCGC CTCCACCAAC 
GTGTCTGCCT CTGACCGCTT CGCTGCCTCT AATTTCGGGA AAGAGCGCTT TGTGAAACTT 
CTGGACCAGC TACACAATTC TCTGCGGATC GACCTGTCCA TGTACCGGAA CAACTTCCCA 
GCCAGCAGCC CTGAGCGGCT GCAGGATCTC AAGTCCACGG TGGACCTGCT CACCAGCATC 
ACCTTCTTCC GGATGAAGGT TCAGGAACTG CAGAGCCCGC CGCGTGCCAG CCAGGTAGTG 
AAAGACTGTG TGAAGGCCTG CCTCAACTCC ACCTATGAGT ACATCTTCAA CAACTGCCAT 
GAGCTCTATG GCCGGGAGTA CCAAACGGAT CCGGCCAAGA AGGGGGAGGT TCCCCCAGAG 
GAGCAAGGCC CTAGCATCAA GAACCTGGAT TTCTGGTCCA AGCTGATCAC CCTCATCGTG 
TCTATCATCG AGGAGGATAA GAATTCTTAC ACACCCTGCC TCAATCAGTT TCCCCAGGAG 
CTCAATGTGG GGAAGATCAG TGCTGAGGTG ATGTGGAGCC TATTTGCCCA AGACATGAAG 
TACGCCATGG AGGAACATGA CAAACACCGG CTGTGTAAGA GCGCAGATTA CATGAACCTG 
CACTTCAAGG TGAAGTGGCT GTACAATGAG TACGTGGCCG AGCTGCCCAC CTTCAAGGAC 
CGTGTGCCTG AGTACCCTGC GTGGTTTGAG CCCTTCGTCA TCCAGTGGTT GGATGAGAAT 
GAGGAGGTGT CCCGGGACTT CCTGCATGGT GCCCTCGAGC GGGACAAGAA GGACGGGTTC 
CAGCAGACCT CAGAGCACGC CCTGTTCTCT TGCTCAGTGG TGGACGTCTT CTCCCAGCTT 
AACCAGAGTT TCGAGATCAT CAAGAAGCTG GAGTGTCCTG ACCCCCAGAT CGTGGGCCAT 
TACATGCGGC GTTTTGCCAA GACCATTAGC AATGTGCTTC TCCAGTATGC AGACATCGTT 
TCCAAGGACT TCGCCTCCTA CTGCTCCAAG GAGAAGGAGA AAGTGCCCTG CATCCTCATG 
AACAACACAC AGCAACTACG AGTGCAGCTG GAGAAGATGT TTGAGGCGAT GGGCGGGAAG 
GAGCTGGATG CCGAGGCCAG TGGCACCCTG AAGGAGCTGC AGGTGAAACT CAACAATGTC 
CTGGATGAAC TCAGCCATGT GTTTGCCACC AGCTTCCAGC CACACATTGA GGAATGCGTC 
AGACAGATGG GTGACATCCT GAGCCAGGTG AAGGGCACAG GCAATGTACC TGCCAGTGCC 
TGCAGCAGCG TGGCCCAGGA TGCAGACAAT GTGCTGCAGC CCATCATGGA TCTTCTGGAC 
AGCAACCTCA CCCTGTTTGC CAAAATCTGT GAGAAGACAG TTCTGAAGCG GGTGCTGAAG 
GAGCTCTGGA AGCTGGTGAT GAACACAATG GAGAAGACCA TCGTCCTGCC ACCGCTCACT 
GACCAGACGA TGATTGGCAC CCTCTTGAGA AAACATGGCA AGGGCCTAGA AAAGGGCAGG 
GTGAAACTGC CAAGCCACTC AGACGGGACA CAAATGATCT TCAATGCTGC CAAGGAGCTG 
GGCCAGCTGT CCAAACTTAA GGATCACATG GTGCGGGAAG AAGCCAAGAG CTTGACCCCG 
AAGCAGTGTG CTGTTGTTGA ACTGGCCCTG GACACCATCA AGCAATACTT CCACGCGGGG 
GGCGTTGGCC TCAAGAAGAC CTTCCTGGAG AAGAGCCCGG ACCTCCAGTC CCTGCGCTAC 
GCCCTGTCGC TCTACACGCA AGCCACCGAC CTGCTCATCA AAACCTTCGT GCAGACGCAG 
TCAGCGCAGG TCCATGGTGG AAAAGGGACT AGGTTTACCC TTAGTGAAGA CGTTTGTCCT 
GAGATGGGCT CTGGTGTGGA GGACCCTGTA GGTGAAGTGT CCGTCCACGT GGAGCTGTTC 
ACACATCCAG GAACTGGGGA ACAGAAGGTC ACAGTGAAGG TGGTGGCCGC CAATGACCTC 
AAGTGGCAGA CTTCTGGCAT CTTCCGCCCG TTCATCGAGG TCAACATCGT TGGACCTCAG 
CTTAGCGACA AGAAACGCAA GTTCGCCACC AAATCCAAAA ACAACAGCTG GGCGCCCAAG 
TATAACGAGA GCTTCCAGTT CTCCCTGAGC GCCGACGCGG GCCCCGAGTG CTACGAGCTG 
CAGGTGTGCG TGAAGGACTA CTGCTTTGCG CGCGAAGACC GCACGGTGGG GCTGGCGGTG 
CTGCAACTGC GAGAGCTGGC CCAGCGCGGG AGCGCCGCGT GCTGGCTTCC ACTCGGCCGC 
CGCATCCACA TGGACGACAC GGGGCTCACA GTGCTGCGCA TCCTGTCGCA GCGCAGCAAC 
GATGAGGTGG CCAAGGAATT CGTCAAGCTC AAGTCGGACA CGCGCTCAGC CGAGGAGGGC 
GGTGCCGCGC CTGCGCCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006509756.1
CDS	126..5306
Translation	MSLLCVGEKFNTYVTLKVQNVKSTTIAVRGSQPSWEQDFMFEINRLDLGLTVEVWNKGLIWDTMVGTVWIPLRTIRQSNEEGPGEWLTLDSQAIMADSEICGTKDPTFHRILLDAHFELPLDIPEEEARYWAKKLEQLNAMRDQDEVSHLQYSFQDQQDKPLPVPSSQCCNWNYFGWGEQNDDPDSAVDDRDSDYRSETSNSIPPPYYTTSQPNASVHQYSVRPPPLGSRESYSDSMHSYEEFSEPRALSPTGSSRYASSGELSQGSSQLSEDFDPDEHSLQGSELDDERDRDSYHSCHSSVSYHKDSPRWDQDDEDLEDLEDLEDEELPEEEEELEEEGEEELEEEDLEEEEEVPDDLASYTQQEDTTVAEPKEFKRISFPTAAPQKDDKVSAVPTEAPEVAKGIPKAATPEEKAAAERAQEAEPPKSEESFRSREEEEGQEGQDAMSRAKANWLRAFNKVRMQLQEARGEGDMSKSLWFKGGPGGGLIIIDSMPDIRKRKPIPLVSDLAMSLVQSRKAGITSALASSTLNNEELKNHVYKKTLQALIYPISCTTPHNFEVWTATTPTYCYECEGLLWGIARQGMRCTECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMVLKDRMKIRERNKPEIFELIQEIFAVTKSAHTQQMKAVKQSVLDGTSKWSAKISITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIYGNLNPVWEENFHFECHNSSDRIKVRVWDEDDDIKSRVKQRFKRESDDFLGQTIIEVRTLSGEMDVWYNLDKRTDKSAVSGAIRLHISVEIKGEEKVAPYHVQYTCLHENLFHFVTDVQNNGVVKIPDAKGDDAWKVYYDETAQEIVDEFAMRYGVESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSMYRNNFPASSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHELYGREYQTDPAKKGEVPPEEQGPSIKNLDFWSKLITLIVSIIEEDKNSYTPCLNQFPQELNVGKISAEVMWSLFAQDMKYAMEEHDKHRLCKSADYMNLHFKVKWLYNEYVAELPTFKDRVPEYPAWFEPFVIQWLDENEEVSRDFLHGALERDKKDGFQQTSEHALFSCSVVDVFSQLNQSFEIIKKLECPDPQIVGHYMRRFAKTISNVLLQYADIVSKDFASYCSKEKEKVPCILMNNTQQLRVQLEKMFEAMGGKELDAEASGTLKELQVKLNNVLDELSHVFATSFQPHIEECVRQMGDILSQVKGTGNVPASACSSVAQDADNVLQPIMDLLDSNLTLFAKICEKTVLKRVLKELWKLVMNTMEKTIVLPPLTDQTMIGTLLRKHGKGLEKGRVKLPSHSDGTQMIFNAAKELGQLSKLKDHMVREEAKSLTPKQCAVVELALDTIKQYFHAGGVGLKKTFLEKSPDLQSLRYALSLYTQATDLLIKTFVQTQSAQVHGGKGTRFTLSEDVCPEMGSGVEDPVGEVSVHVELFTHPGTGEQKVTVKVVAANDLKWQTSGIFRPFIEVNIVGPQLSDKKRKFATKSKNNSWAPKYNESFQFSLSADAGPECYELQVCVKDYCFAREDRTVGLAVLQLRELAQRGSAACWLPLGRRIHMDDTGLTVLRILSQRSNDEVAKEFVKLKSDTRSAEEGGAAPAP

Target ORF information:

RefSeq Version	XM_006509693.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus unc-13 homolog A (C. elegans) (Unc13a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006509693.3

ORF Insert Sequence:

ATGTCTCTGC TGTGCGTGGG AGAGAAGTTC AACACATACG TGACGCTGAA GGTGCAGAAC 
GTGAAGAGCA CTACCATAGC TGTACGTGGC AGCCAGCCCA GCTGGGAGCA GGACTTCATG 
TTTGAGATCA ACCGCCTGGA TCTAGGCCTG ACCGTGGAGG TGTGGAACAA GGGTCTCATC 
TGGGACACGA TGGTGGGTAC TGTGTGGATC CCGCTGCGGA CCATCCGCCA GTCCAATGAG 
GAGGGCCCAG GAGAGTGGCT CACACTGGAC TCTCAGGCCA TCATGGCGGA CAGTGAGATC 
TGTGGGACCA AGGACCCCAC CTTCCATCGC ATCCTCCTGG ACGCTCATTT CGAGCTGCCG 
TTGGACATCC CTGAGGAGGA GGCACGCTAC TGGGCCAAGA AGCTGGAGCA GCTGAATGCC 
ATGCGTGACC AGGATGAGGT GAGTCACCTC CAGTACTCCT TCCAGGACCA GCAGGACAAG 
CCGCTGCCAG TGCCCAGCAG CCAGTGCTGC AACTGGAATT ACTTTGGCTG GGGAGAACAG 
AATGACGACC CCGACAGTGC GGTGGATGAC CGGGACAGTG ACTATAGGAG TGAGACCAGC 
AACAGCATCC CACCGCCCTA CTATACGACT TCGCAGCCCA ACGCCTCTGT GCACCAGTAC 
TCCGTGCGAC CGCCCCCTCT GGGGTCCCGG GAGTCCTACA GTGACTCCAT GCACAGCTAT 
GAGGAGTTCT CTGAGCCGCG GGCGCTCAGC CCCACAGGCA GCAGCCGCTA TGCTTCTTCC 
GGGGAGCTGA GCCAGGGAAG CTCACAGCTG AGCGAGGACT TCGACCCTGA TGAGCACAGC 
CTGCAGGGCT CGGAGCTGGA TGACGAGAGG GACCGCGATT CTTATCACTC CTGTCACAGC 
TCCGTGAGTT ACCACAAGGA CTCACCCCGC TGGGACCAGG ACGATGAGGA CCTGGAGGAC 
CTGGAGGACC TGGAAGATGA GGAGCTGCCC GAGGAGGAGG AGGAGTTGGA GGAGGAGGGG 
GAGGAGGAAT TGGAGGAAGA GGATTTGGAG GAGGAGGAGG AGGTGCCTGA TGACCTGGCC 
AGCTACACCC AGCAGGAGGA CACCACTGTG GCTGAGCCCA AAGAGTTCAA GCGGATCAGC 
TTCCCAACGG CTGCGCCTCA GAAGGATGAC AAGGTTTCAG CTGTGCCTAC AGAGGCCCCC 
GAGGTGGCCA AAGGCATCCC CAAAGCAGCC ACACCCGAGG AGAAGGCAGC TGCAGAGCGT 
GCGCAGGAAG CCGAGCCCCC CAAGTCTGAG GAGAGTTTCA GATCTCGAGA GGAGGAAGAG 
GGCCAGGAAG GGCAGGATGC CATGTCCAGA GCCAAAGCCA ACTGGTTGCG AGCCTTCAAC 
AAGGTGCGCA TGCAGCTGCA GGAGGCCCGA GGAGAAGGGG ATATGTCTAA GTCTCTATGG 
TTCAAAGGCG GTCCTGGTGG TGGCCTTATC ATCATTGACA GCATGCCAGA CATCAGGAAA 
CGGAAGCCCA TTCCCCTCGT GAGCGACCTG GCTATGTCAT TGGTGCAGTC TCGGAAGGCA 
GGCATCACCT CGGCCTTGGC CTCCAGCACG TTGAACAATG AAGAGCTGAA AAATCACGTT 
TACAAGAAGA CCCTGCAAGC CTTAATCTAC CCCATCTCCT GCACCACGCC GCACAATTTC 
GAGGTGTGGA CGGCCACCAC GCCCACCTAC TGCTATGAGT GCGAGGGGCT GCTGTGGGGC 
ATCGCGCGGC AGGGCATGCG ATGCACCGAG TGCGGCGTTA AGTGCCACGA GAAGTGCCAG 
GACCTGCTCA ATGCGGATTG CCTGCAGCGG GCGGCTGAGA AGAGTTCCAA GCATGGCGCT 
GAAGACCGCA CGCAGAACAT CATCATGGTG CTGAAGGACC GCATGAAGAT CCGCGAGCGC 
AACAAGCCTG AGATCTTCGA GCTTATCCAA GAGATCTTCG CGGTCACCAA GAGCGCACAC 
ACACAGCAGA TGAAAGCTGT CAAGCAGAGT GTGCTGGACG GCACATCCAA GTGGTCTGCC 
AAAATTAGCA TCACAGTGGT CTGTGCCCAG GGCTTGCAGG CCAAGGACAA GACAGGATCC 
AGTGACCCTT ATGTCACCGT CCAGGTTGGG AAGACCAAGA AAAGGACAAA AACCATCTAC 
GGGAACCTCA ACCCAGTGTG GGAAGAGAAT TTTCATTTTG AATGTCACAA CTCCTCTGAC 
CGGATCAAGG TTCGTGTTTG GGATGAAGAT GACGACATAA AATCCCGTGT GAAACAAAGG 
TTTAAGAGGG AGTCTGATGA CTTCCTAGGG CAGACAATCA TCGAGGTGCG GACGCTTAGC 
GGCGAGATGG ATGTGTGGTA CAATCTGGAC AAGAGAACGG ACAAGTCGGC GGTGTCGGGG 
GCCATCCGGC TTCACATCAG TGTGGAGATC AAAGGGGAGG AGAAGGTGGC GCCCTACCAT 
GTCCAGTACA CCTGTCTGCA TGAGAACCTG TTCCACTTTG TGACGGACGT GCAGAACAAT 
GGTGTGGTGA AGATTCCCGA TGCCAAGGGT GACGACGCCT GGAAGGTTTA CTACGATGAG 
ACGGCCCAGG AGATCGTGGA TGAGTTTGCC ATGCGCTATG GTGTTGAGTC CATCTACCAA 
GCCATGACCC ACTTTGCCTG CCTCTCCTCC AAGTATATGT GCCCTGGGGT ACCCGCTGTC 
ATGAGCACCC TGCTTGCCAA CATCAACGCC TACTACGCAC ACACCACCGC CTCCACCAAC 
GTGTCTGCCT CTGACCGCTT CGCTGCCTCT AATTTCGGGA AAGAGCGCTT TGTGAAACTT 
CTGGACCAGC TACACAATTC TCTGCGGATC GACCTGTCCA TGTACCGGAA CAACTTCCCA 
GCCAGCAGCC CTGAGCGGCT GCAGGATCTC AAGTCCACGG TGGACCTGCT CACCAGCATC 
ACCTTCTTCC GGATGAAGGT TCAGGAACTG CAGAGCCCGC CGCGTGCCAG CCAGGTAGTG 
AAAGACTGTG TGAAGGCCTG CCTCAACTCC ACCTATGAGT ACATCTTCAA CAACTGCCAT 
GAGCTCTATG GCCGGGAGTA CCAAACGGAT CCGGCCAAGA AGGGGGAGGT TCCCCCAGAG 
GAGCAAGGCC CTAGCATCAA GAACCTGGAT TTCTGGTCCA AGCTGATCAC CCTCATCGTG 
TCTATCATCG AGGAGGATAA GAATTCTTAC ACACCCTGCC TCAATCAGTT TCCCCAGGAG 
CTCAATGTGG GGAAGATCAG TGCTGAGGTG ATGTGGAGCC TATTTGCCCA AGACATGAAG 
TACGCCATGG AGGAACATGA CAAACACCGG CTGTGTAAGA GCGCAGATTA CATGAACCTG 
CACTTCAAGG TGAAGTGGCT GTACAATGAG TACGTGGCCG AGCTGCCCAC CTTCAAGGAC 
CGTGTGCCTG AGTACCCTGC GTGGTTTGAG CCCTTCGTCA TCCAGTGGTT GGATGAGAAT 
GAGGAGGTGT CCCGGGACTT CCTGCATGGT GCCCTCGAGC GGGACAAGAA GGACGGGTTC 
CAGCAGACCT CAGAGCACGC CCTGTTCTCT TGCTCAGTGG TGGACGTCTT CTCCCAGCTT 
AACCAGAGTT TCGAGATCAT CAAGAAGCTG GAGTGTCCTG ACCCCCAGAT CGTGGGCCAT 
TACATGCGGC GTTTTGCCAA GACCATTAGC AATGTGCTTC TCCAGTATGC AGACATCGTT 
TCCAAGGACT TCGCCTCCTA CTGCTCCAAG GAGAAGGAGA AAGTGCCCTG CATCCTCATG 
AACAACACAC AGCAACTACG AGTGCAGCTG GAGAAGATGT TTGAGGCGAT GGGCGGGAAG 
GAGCTGGATG CCGAGGCCAG TGGCACCCTG AAGGAGCTGC AGGTGAAACT CAACAATGTC 
CTGGATGAAC TCAGCCATGT GTTTGCCACC AGCTTCCAGC CACACATTGA GGAATGCGTC 
AGACAGATGG GTGACATCCT GAGCCAGGTG AAGGGCACAG GCAATGTACC TGCCAGTGCC 
TGCAGCAGCG TGGCCCAGGA TGCAGACAAT GTGCTGCAGC CCATCATGGA TCTTCTGGAC 
AGCAACCTCA CCCTGTTTGC CAAAATCTGT GAGAAGACAG TTCTGAAGCG GGTGCTGAAG 
GAGCTCTGGA AGCTGGTGAT GAACACAATG GAGAAGACCA TCGTCCTGCC ACCGCTCACT 
GACCAGACGA TGATTGGCAC CCTCTTGAGA AAACATGGCA AGGGCCTAGA AAAGGGCAGG 
GTGAAACTGC CAAGCCACTC AGACGGGACA CAAATGATCT TCAATGCTGC CAAGGAGCTG 
GGCCAGCTGT CCAAACTTAA GGATCACATG GTGCGGGAAG AAGCCAAGAG CTTGACCCCG 
AAGCAGTGTG CTGTTGTTGA ACTGGCCCTG GACACCATCA AGCAATACTT CCACGCGGGG 
GGCGTTGGCC TCAAGAAGAC CTTCCTGGAG AAGAGCCCGG ACCTCCAGTC CCTGCGCTAC 
GCCCTGTCGC TCTACACGCA AGCCACCGAC CTGCTCATCA AAACCTTCGT GCAGACGCAG 
TCAGCGCAGG TCCATGGTGG AAAAGGGACT AGGTTTACCC TTAGTGAAGA CGTTTGTCCT 
GAGATGGGCT CTGGTGTGGA GGACCCTGTA GGTGAAGTGT CCGTCCACGT GGAGCTGTTC 
ACACATCCAG GAACTGGGGA ACAGAAGGTC ACAGTGAAGG TGGTGGCCGC CAATGACCTC 
AAGTGGCAGA CTTCTGGCAT CTTCCGCCCG TTCATCGAGG TCAACATCGT TGGACCTCAG 
CTTAGCGACA AGAAACGCAA GTTCGCCACC AAATCCAAAA ACAACAGCTG GGCGCCCAAG 
TATAACGAGA GCTTCCAGTT CTCCCTGAGC GCCGACGCGG GCCCCGAGTG CTACGAGCTG 
CAGGTGTGCG TGAAGGACTA CTGCTTTGCG CGCGAAGACC GCACGGTGGG GCTGGCGGTG 
CTGCAACTGC GAGAGCTGGC CCAGCGCGGG AGCGCCGCGT GCTGGCTTCC ACTCGGCCGC 
CGCATCCACA TGGACGACAC GGGGCTCACA GTGCTGCGCA TCCTGTCGCA GCGCAGCAAC 
GATGAGGTGG CCAAGGAATT CGTCAAGCTC AAGTCGGACA CGCGCTCAGC CGAGGAGGGC 
GGTGCCGCGC CTGCGCCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Unc13a ( XM_017312874.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168363.1 Mus musculus unc-13 homolog A (C. elegans) (Unc13a), transcript variant X1, mRNA.

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CloneID	OMu82525
Clone ID Related Accession (Same CDS sequence)	XM_017312874.2 , XM_017312874.1
Accession Version	XM_017312874.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5211bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein unc-13 homolog A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTGC TGTGCGTGGG AGTCAAAAAA GCCAAGTTTG ACGGTGCCCA AGAGAAGTTC 
AACACATACG TGACGCTGAA GGTGCAGAAC GTGAAGAGCA CTACCATAGC TGTACGTGGC 
AGCCAGCCCA GCTGGGAGCA GGACTTCATG TTTGAGATCA ACCGCCTGGA TCTAGGCCTG 
ACCGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGTAC TGTGTGGATC 
CCGCTGCGGA CCATCCGCCA GTCCAATGAG GAGGGCCCAG GAGAGTGGCT CACACTGGAC 
TCTCAGGCCA TCATGGCGGA CAGTGAGATC TGTGGGACCA AGGACCCCAC CTTCCATCGC 
ATCCTCCTGG ACGCTCATTT CGAGCTGCCG TTGGACATCC CTGAGGAGGA GGCACGCTAC 
TGGGCCAAGA AGCTGGAGCA GCTGAATGCC ATGCGTGACC AGGATGAGGT GAGTCACCTC 
CAGTACTCCT TCCAGGACCA GCAGGACAAG CCGCTGCCAG TGCCCAGCAG CCAGTGCTGC 
AACTGGAATT ACTTTGGCTG GGGAGAACAG AATGACGACC CCGACAGTGC GGTGGATGAC 
CGGGACAGTG ACTATAGGAG TGAGACCAGC AACAGCATCC CACCGCCCTA CTATACGACT 
TCGCAGCCCA ACGCCTCTGT GCACCAGTAC TCCGTGCGAC CGCCCCCTCT GGGGTCCCGG 
GAGTCCTACA GTGACTCCAT GCACAGCTAT GAGGAGTTCT CTGAGCCGCG GGCGCTCAGC 
CCCACAGGCA GCAGCCGCTA TGCTTCTTCC GGGGAGCTGA GCCAGGGAAG CTCACAGCTG 
AGCGAGGACT TCGACCCTGA TGAGCACAGC CTGCAGGGCT CGGAGCTGGA TGACGAGAGG 
GACCGCGATT CTTATCACTC CTGTCACAGC TCCGTGAGTT ACCACAAGGA CTCACCCCGC 
TGGGACCAGG ACGATGAGGA CCTGGAGGAC CTGGAGGACC TGGAAGATGA GGAGCTGCCC 
GAGGAGGAGG AGGAGTTGGA GGAGGAGGGG GAGGAGGAAT TGGAGGAAGA GGATTTGGAG 
GAGGAGGAGG AGGTGCCTGA TGACCTGGCC AGCTACACCC AGCAGGAGGA CACCACTGTG 
GCTGAGCCCA AAGAGTTCAA GCGGATCAGC TTCCCAACGG CTGCGCCTCA GAAGGATGAC 
AAGGTTTCAG CTGTGCCTAC AGAGGCCCCC GAGGTGGCCA AAGGCATCCC CAAAGCAGCC 
ACACCCGAGG AGAAGGCAGC TGCAGAGCGT GCGCAGGAAG CCGAGCCCCC CAAGTCTGAG 
GAGAGTTTCA GATCTCGAGA GGAGGAAGAG GGCCAGGAAG GGCAGGATGC CATGTCCAGA 
GCCAAAGCCA ACTGGTTGCG AGCCTTCAAC AAGGTGCGCA TGCAGCTGCA GGAGGCCCGA 
GGAGAAGGGG ATATGTCTAA GTCTCTATGG TTCAAAGGCG GTCCTGGTGG TGGCCTTATC 
ATCATTGACA GCATGCCAGA CATCAGGAAA CGGAAGCCCA TTCCCCTCGT GAGCGACCTG 
GCTATGTCAT TGGTGCAGTC TCGGAAGGCA GGCATCACCT CGGCCTTGGC CTCCAGCACG 
TTGAACAATG AAGAGCTGAA AAATCACGTT TACAAGAAGA CCCTGCAAGC CTTAATCTAC 
CCCATCTCCT GCACCACGCC GCACAATTTC GAGGTGTGGA CGGCCACCAC GCCCACCTAC 
TGCTATGAGT GCGAGGGGCT GCTGTGGGGC ATCGCGCGGC AGGGCATGCG ATGCACCGAG 
TGCGGCGTTA AGTGCCACGA GAAGTGCCAG GACCTGCTCA ATGCGGATTG CCTGCAGCGG 
GCGGCTGAGA AGAGTTCCAA GCATGGCGCT GAAGACCGCA CGCAGAACAT CATCATGGTG 
CTGAAGGACC GCATGAAGAT CCGCGAGCGC AACAAGCCTG AGATCTTCGA GCTTATCCAA 
GAGATCTTCG CGGTCACCAA GAGCGCACAC ACACAGCAGA TGAAAGCTGT CAAGCAGAGT 
GTGCTGGACG GCACATCCAA GTGGTCTGCC AAAATTAGCA TCACAGTGGT CTGTGCCCAG 
GGCTTGCAGG CCAAGGACAA GACAGGATCC AGTGACCCTT ATGTCACCGT CCAGGTTGGG 
AAGACCAAGA AAAGGACAAA AACCATCTAC GGGAACCTCA ACCCAGTGTG GGAAGAGAAT 
TTTCATTTTG AATGTCACAA CTCCTCTGAC CGGATCAAGG TTCGTGTTTG GGATGAAGAT 
GACGACATAA AATCCCGTGT GAAACAAAGG TTTAAGAGGG AGTCTGATGA CTTCCTAGGG 
CAGACAATCA TCGAGGTGCG GACGCTTAGC GGCGAGATGG ATGTGTGGTA CAATCTGGAC 
AAGAGAACGG ACAAGTCGGC GGTGTCGGGG GCCATCCGGC TTCACATCAG TGTGGAGATC 
AAAGGGGAGG AGAAGGTGGC GCCCTACCAT GTCCAGTACA CCTGTCTGCA TGAGAACCTG 
TTCCACTTTG TGACGGACGT GCAGAACAAT GGTGTGGTGA AGATTCCCGA TGCCAAGGGT 
GACGACGCCT GGAAGGTTTA CTACGATGAG ACGGCCCAGG AGATCGTGGA TGAGTTTGCC 
ATGCGCTATG GTGTTGAGTC CATCTACCAA GCCATGACCC ACTTTGCCTG CCTCTCCTCC 
AAGTATATGT GCCCTGGGGT ACCCGCTGTC ATGAGCACCC TGCTTGCCAA CATCAACGCC 
TACTACGCAC ACACCACCGC CTCCACCAAC GTGTCTGCCT CTGACCGCTT CGCTGCCTCT 
AATTTCGGGA AAGAGCGCTT TGTGAAACTT CTGGACCAGC TACACAATTC TCTGCGGATC 
GACCTGTCCA TGTACCGGAA CAACTTCCCA GCCAGCAGCC CTGAGCGGCT GCAGGATCTC 
AAGTCCACGG TGGACCTGCT CACCAGCATC ACCTTCTTCC GGATGAAGGT TCAGGAACTG 
CAGAGCCCGC CGCGTGCCAG CCAGGTAGTG AAAGACTGTG TGAAGGCCTG CCTCAACTCC 
ACCTATGAGT ACATCTTCAA CAACTGCCAT GAGCTCTATG GCCGGGAGTA CCAAACGGAT 
CCGGCCAAGA AGGGGGAGGT TCCCCCAGAG GAGCAAGGCC CTAGCATCAA GAACCTGGAT 
TTCTGGTCCA AGCTGATCAC CCTCATCGTG TCTATCATCG AGGAGGATAA GAATTCTTAC 
ACACCCTGCC TCAATCAGTT TCCCCAGGAG CTCAATGTGG GGAAGATCAG TGCTGAGGTG 
ATGTGGAGCC TATTTGCCCA AGACATGAAG TACGCCATGG AGGAACATGA CAAACACCGG 
CTGTGTAAGA GCGCAGATTA CATGAACCTG CACTTCAAGG TGAAGTGGCT GTACAATGAG 
TACGTGGCCG AGCTGCCCAC CTTCAAGGAC CGTGTGCCTG AGTACCCTGC GTGGTTTGAG 
CCCTTCGTCA TCCAGTGGTT GGATGAGAAT GAGGAGGTGT CCCGGGACTT CCTGCATGGT 
GCCCTCGAGC GGGACAAGAA GGACGGGTTC CAGCAGACCT CAGAGCACGC CCTGTTCTCT 
TGCTCAGTGG TGGACGTCTT CTCCCAGCTT AACCAGAGTT TCGAGATCAT CAAGAAGCTG 
GAGTGTCCTG ACCCCCAGAT CGTGGGCCAT TACATGCGGC GTTTTGCCAA GACCATTAGC 
AATGTGCTTC TCCAGTATGC AGACATCGTT TCCAAGGACT TCGCCTCCTA CTGCTCCAAG 
GAGAAGGAGA AAGTGCCCTG CATCCTCATG AACAACACAC AGCAACTACG AGTGCAGCTG 
GAGAAGATGT TTGAGGCGAT GGGCGGGAAG GAGCTGGATG CCGAGGCCAG TGGCACCCTG 
AAGGAGCTGC AGGTGAAACT CAACAATGTC CTGGATGAAC TCAGCCATGT GTTTGCCACC 
AGCTTCCAGC CACACATTGA GGAATGCGTC AGACAGATGG GTGACATCCT GAGCCAGGTG 
AAGGGCACAG GCAATGTACC TGCCAGTGCC TGCAGCAGCG TGGCCCAGGA TGCAGACAAT 
GTGCTGCAGC CCATCATGGA TCTTCTGGAC AGCAACCTCA CCCTGTTTGC CAAAATCTGT 
GAGAAGACAG TTCTGAAGCG GGTGCTGAAG GAGCTCTGGA AGCTGGTGAT GAACACAATG 
GAGAAGACCA TCGTCCTGCC ACCGCTCACT GACCAGACGA TGATTGGCAC CCTCTTGAGA 
AAACATGGCA AGGGCCTAGA AAAGGGCAGG GTGAAACTGC CAAGCCACTC AGACGGGACA 
CAAATGATCT TCAATGCTGC CAAGGAGCTG GGCCAGCTGT CCAAACTTAA GGATCACATG 
GTGCGGGAAG AAGCCAAGAG CTTGACCCCG AAGCAGTGTG CTGTTGTTGA ACTGGCCCTG 
GACACCATCA AGCAATACTT CCACGCGGGG GGCGTTGGCC TCAAGAAGAC CTTCCTGGAG 
AAGAGCCCGG ACCTCCAGTC CCTGCGCTAC GCCCTGTCGC TCTACACGCA AGCCACCGAC 
CTGCTCATCA AAACCTTCGT GCAGACGCAG TCAGCGCAGG TCCATGGTGG AAAAGGGACT 
AGGTTTACCC TTAGTGAAGA CGTTTGTCCT GAGATGGGCT CTGGTGTGGA GGACCCTGTA 
GGTGAAGTGT CCGTCCACGT GGAGCTGTTC ACACATCCAG GAACTGGGGA ACAGAAGGTC 
ACAGTGAAGG TGGTGGCCGC CAATGACCTC AAGTGGCAGA CTTCTGGCAT CTTCCGCCCG 
TTCATCGAGG TCAACATCGT TGGACCTCAG CTTAGCGACA AGAAACGCAA GTTCGCCACC 
AAATCCAAAA ACAACAGCTG GGCGCCCAAG TATAACGAGA GCTTCCAGTT CTCCCTGAGC 
GCCGACGCGG GCCCCGAGTG CTACGAGCTG CAGGTGTGCG TGAAGGACTA CTGCTTTGCG 
CGCGAAGACC GCACGGTGGG GCTGGCGGTG CTGCAACTGC GAGAGCTGGC CCAGCGCGGG 
AGCGCCGCGT GCTGGCTTCC ACTCGGCCGC CGCATCCACA TGGACGACAC GGGGCTCACA 
GTGCTGCGCA TCCTGTCGCA GCGCAGCAAC GATGAGGTGG CCAAGGAATT CGTCAAGCTC 
AAGTCGGACA CGCGCTCAGC CGAGGAGGGC GGTGCCGCGC CTGCGCCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168363.1
CDS	126..5336
Translation	MSLLCVGVKKAKFDGAQEKFNTYVTLKVQNVKSTTIAVRGSQPSWEQDFMFEINRLDLGLTVEVWNKGLIWDTMVGTVWIPLRTIRQSNEEGPGEWLTLDSQAIMADSEICGTKDPTFHRILLDAHFELPLDIPEEEARYWAKKLEQLNAMRDQDEVSHLQYSFQDQQDKPLPVPSSQCCNWNYFGWGEQNDDPDSAVDDRDSDYRSETSNSIPPPYYTTSQPNASVHQYSVRPPPLGSRESYSDSMHSYEEFSEPRALSPTGSSRYASSGELSQGSSQLSEDFDPDEHSLQGSELDDERDRDSYHSCHSSVSYHKDSPRWDQDDEDLEDLEDLEDEELPEEEEELEEEGEEELEEEDLEEEEEVPDDLASYTQQEDTTVAEPKEFKRISFPTAAPQKDDKVSAVPTEAPEVAKGIPKAATPEEKAAAERAQEAEPPKSEESFRSREEEEGQEGQDAMSRAKANWLRAFNKVRMQLQEARGEGDMSKSLWFKGGPGGGLIIIDSMPDIRKRKPIPLVSDLAMSLVQSRKAGITSALASSTLNNEELKNHVYKKTLQALIYPISCTTPHNFEVWTATTPTYCYECEGLLWGIARQGMRCTECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMVLKDRMKIRERNKPEIFELIQEIFAVTKSAHTQQMKAVKQSVLDGTSKWSAKISITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIYGNLNPVWEENFHFECHNSSDRIKVRVWDEDDDIKSRVKQRFKRESDDFLGQTIIEVRTLSGEMDVWYNLDKRTDKSAVSGAIRLHISVEIKGEEKVAPYHVQYTCLHENLFHFVTDVQNNGVVKIPDAKGDDAWKVYYDETAQEIVDEFAMRYGVESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSMYRNNFPASSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHELYGREYQTDPAKKGEVPPEEQGPSIKNLDFWSKLITLIVSIIEEDKNSYTPCLNQFPQELNVGKISAEVMWSLFAQDMKYAMEEHDKHRLCKSADYMNLHFKVKWLYNEYVAELPTFKDRVPEYPAWFEPFVIQWLDENEEVSRDFLHGALERDKKDGFQQTSEHALFSCSVVDVFSQLNQSFEIIKKLECPDPQIVGHYMRRFAKTISNVLLQYADIVSKDFASYCSKEKEKVPCILMNNTQQLRVQLEKMFEAMGGKELDAEASGTLKELQVKLNNVLDELSHVFATSFQPHIEECVRQMGDILSQVKGTGNVPASACSSVAQDADNVLQPIMDLLDSNLTLFAKICEKTVLKRVLKELWKLVMNTMEKTIVLPPLTDQTMIGTLLRKHGKGLEKGRVKLPSHSDGTQMIFNAAKELGQLSKLKDHMVREEAKSLTPKQCAVVELALDTIKQYFHAGGVGLKKTFLEKSPDLQSLRYALSLYTQATDLLIKTFVQTQSAQVHGGKGTRFTLSEDVCPEMGSGVEDPVGEVSVHVELFTHPGTGEQKVTVKVVAANDLKWQTSGIFRPFIEVNIVGPQLSDKKRKFATKSKNNSWAPKYNESFQFSLSADAGPECYELQVCVKDYCFAREDRTVGLAVLQLRELAQRGSAACWLPLGRRIHMDDTGLTVLRILSQRSNDEVAKEFVKLKSDTRSAEEGGAAPAP

Target ORF information:

RefSeq Version	XM_017312874.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus unc-13 homolog A (C. elegans) (Unc13a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312874.1

ORF Insert Sequence:

ATGTCTCTGC TGTGCGTGGG AGTCAAAAAA GCCAAGTTTG ACGGTGCCCA AGAGAAGTTC 
AACACATACG TGACGCTGAA GGTGCAGAAC GTGAAGAGCA CTACCATAGC TGTACGTGGC 
AGCCAGCCCA GCTGGGAGCA GGACTTCATG TTTGAGATCA ACCGCCTGGA TCTAGGCCTG 
ACCGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGTAC TGTGTGGATC 
CCGCTGCGGA CCATCCGCCA GTCCAATGAG GAGGGCCCAG GAGAGTGGCT CACACTGGAC 
TCTCAGGCCA TCATGGCGGA CAGTGAGATC TGTGGGACCA AGGACCCCAC CTTCCATCGC 
ATCCTCCTGG ACGCTCATTT CGAGCTGCCG TTGGACATCC CTGAGGAGGA GGCACGCTAC 
TGGGCCAAGA AGCTGGAGCA GCTGAATGCC ATGCGTGACC AGGATGAGGT GAGTCACCTC 
CAGTACTCCT TCCAGGACCA GCAGGACAAG CCGCTGCCAG TGCCCAGCAG CCAGTGCTGC 
AACTGGAATT ACTTTGGCTG GGGAGAACAG AATGACGACC CCGACAGTGC GGTGGATGAC 
CGGGACAGTG ACTATAGGAG TGAGACCAGC AACAGCATCC CACCGCCCTA CTATACGACT 
TCGCAGCCCA ACGCCTCTGT GCACCAGTAC TCCGTGCGAC CGCCCCCTCT GGGGTCCCGG 
GAGTCCTACA GTGACTCCAT GCACAGCTAT GAGGAGTTCT CTGAGCCGCG GGCGCTCAGC 
CCCACAGGCA GCAGCCGCTA TGCTTCTTCC GGGGAGCTGA GCCAGGGAAG CTCACAGCTG 
AGCGAGGACT TCGACCCTGA TGAGCACAGC CTGCAGGGCT CGGAGCTGGA TGACGAGAGG 
GACCGCGATT CTTATCACTC CTGTCACAGC TCCGTGAGTT ACCACAAGGA CTCACCCCGC 
TGGGACCAGG ACGATGAGGA CCTGGAGGAC CTGGAGGACC TGGAAGATGA GGAGCTGCCC 
GAGGAGGAGG AGGAGTTGGA GGAGGAGGGG GAGGAGGAAT TGGAGGAAGA GGATTTGGAG 
GAGGAGGAGG AGGTGCCTGA TGACCTGGCC AGCTACACCC AGCAGGAGGA CACCACTGTG 
GCTGAGCCCA AAGAGTTCAA GCGGATCAGC TTCCCAACGG CTGCGCCTCA GAAGGATGAC 
AAGGTTTCAG CTGTGCCTAC AGAGGCCCCC GAGGTGGCCA AAGGCATCCC CAAAGCAGCC 
ACACCCGAGG AGAAGGCAGC TGCAGAGCGT GCGCAGGAAG CCGAGCCCCC CAAGTCTGAG 
GAGAGTTTCA GATCTCGAGA GGAGGAAGAG GGCCAGGAAG GGCAGGATGC CATGTCCAGA 
GCCAAAGCCA ACTGGTTGCG AGCCTTCAAC AAGGTGCGCA TGCAGCTGCA GGAGGCCCGA 
GGAGAAGGGG ATATGTCTAA GTCTCTATGG TTCAAAGGCG GTCCTGGTGG TGGCCTTATC 
ATCATTGACA GCATGCCAGA CATCAGGAAA CGGAAGCCCA TTCCCCTCGT GAGCGACCTG 
GCTATGTCAT TGGTGCAGTC TCGGAAGGCA GGCATCACCT CGGCCTTGGC CTCCAGCACG 
TTGAACAATG AAGAGCTGAA AAATCACGTT TACAAGAAGA CCCTGCAAGC CTTAATCTAC 
CCCATCTCCT GCACCACGCC GCACAATTTC GAGGTGTGGA CGGCCACCAC GCCCACCTAC 
TGCTATGAGT GCGAGGGGCT GCTGTGGGGC ATCGCGCGGC AGGGCATGCG ATGCACCGAG 
TGCGGCGTTA AGTGCCACGA GAAGTGCCAG GACCTGCTCA ATGCGGATTG CCTGCAGCGG 
GCGGCTGAGA AGAGTTCCAA GCATGGCGCT GAAGACCGCA CGCAGAACAT CATCATGGTG 
CTGAAGGACC GCATGAAGAT CCGCGAGCGC AACAAGCCTG AGATCTTCGA GCTTATCCAA 
GAGATCTTCG CGGTCACCAA GAGCGCACAC ACACAGCAGA TGAAAGCTGT CAAGCAGAGT 
GTGCTGGACG GCACATCCAA GTGGTCTGCC AAAATTAGCA TCACAGTGGT CTGTGCCCAG 
GGCTTGCAGG CCAAGGACAA GACAGGATCC AGTGACCCTT ATGTCACCGT CCAGGTTGGG 
AAGACCAAGA AAAGGACAAA AACCATCTAC GGGAACCTCA ACCCAGTGTG GGAAGAGAAT 
TTTCATTTTG AATGTCACAA CTCCTCTGAC CGGATCAAGG TTCGTGTTTG GGATGAAGAT 
GACGACATAA AATCCCGTGT GAAACAAAGG TTTAAGAGGG AGTCTGATGA CTTCCTAGGG 
CAGACAATCA TCGAGGTGCG GACGCTTAGC GGCGAGATGG ATGTGTGGTA CAATCTGGAC 
AAGAGAACGG ACAAGTCGGC GGTGTCGGGG GCCATCCGGC TTCACATCAG TGTGGAGATC 
AAAGGGGAGG AGAAGGTGGC GCCCTACCAT GTCCAGTACA CCTGTCTGCA TGAGAACCTG 
TTCCACTTTG TGACGGACGT GCAGAACAAT GGTGTGGTGA AGATTCCCGA TGCCAAGGGT 
GACGACGCCT GGAAGGTTTA CTACGATGAG ACGGCCCAGG AGATCGTGGA TGAGTTTGCC 
ATGCGCTATG GTGTTGAGTC CATCTACCAA GCCATGACCC ACTTTGCCTG CCTCTCCTCC 
AAGTATATGT GCCCTGGGGT ACCCGCTGTC ATGAGCACCC TGCTTGCCAA CATCAACGCC 
TACTACGCAC ACACCACCGC CTCCACCAAC GTGTCTGCCT CTGACCGCTT CGCTGCCTCT 
AATTTCGGGA AAGAGCGCTT TGTGAAACTT CTGGACCAGC TACACAATTC TCTGCGGATC 
GACCTGTCCA TGTACCGGAA CAACTTCCCA GCCAGCAGCC CTGAGCGGCT GCAGGATCTC 
AAGTCCACGG TGGACCTGCT CACCAGCATC ACCTTCTTCC GGATGAAGGT TCAGGAACTG 
CAGAGCCCGC CGCGTGCCAG CCAGGTAGTG AAAGACTGTG TGAAGGCCTG CCTCAACTCC 
ACCTATGAGT ACATCTTCAA CAACTGCCAT GAGCTCTATG GCCGGGAGTA CCAAACGGAT 
CCGGCCAAGA AGGGGGAGGT TCCCCCAGAG GAGCAAGGCC CTAGCATCAA GAACCTGGAT 
TTCTGGTCCA AGCTGATCAC CCTCATCGTG TCTATCATCG AGGAGGATAA GAATTCTTAC 
ACACCCTGCC TCAATCAGTT TCCCCAGGAG CTCAATGTGG GGAAGATCAG TGCTGAGGTG 
ATGTGGAGCC TATTTGCCCA AGACATGAAG TACGCCATGG AGGAACATGA CAAACACCGG 
CTGTGTAAGA GCGCAGATTA CATGAACCTG CACTTCAAGG TGAAGTGGCT GTACAATGAG 
TACGTGGCCG AGCTGCCCAC CTTCAAGGAC CGTGTGCCTG AGTACCCTGC GTGGTTTGAG 
CCCTTCGTCA TCCAGTGGTT GGATGAGAAT GAGGAGGTGT CCCGGGACTT CCTGCATGGT 
GCCCTCGAGC GGGACAAGAA GGACGGGTTC CAGCAGACCT CAGAGCACGC CCTGTTCTCT 
TGCTCAGTGG TGGACGTCTT CTCCCAGCTT AACCAGAGTT TCGAGATCAT CAAGAAGCTG 
GAGTGTCCTG ACCCCCAGAT CGTGGGCCAT TACATGCGGC GTTTTGCCAA GACCATTAGC 
AATGTGCTTC TCCAGTATGC AGACATCGTT TCCAAGGACT TCGCCTCCTA CTGCTCCAAG 
GAGAAGGAGA AAGTGCCCTG CATCCTCATG AACAACACAC AGCAACTACG AGTGCAGCTG 
GAGAAGATGT TTGAGGCGAT GGGCGGGAAG GAGCTGGATG CCGAGGCCAG TGGCACCCTG 
AAGGAGCTGC AGGTGAAACT CAACAATGTC CTGGATGAAC TCAGCCATGT GTTTGCCACC 
AGCTTCCAGC CACACATTGA GGAATGCGTC AGACAGATGG GTGACATCCT GAGCCAGGTG 
AAGGGCACAG GCAATGTACC TGCCAGTGCC TGCAGCAGCG TGGCCCAGGA TGCAGACAAT 
GTGCTGCAGC CCATCATGGA TCTTCTGGAC AGCAACCTCA CCCTGTTTGC CAAAATCTGT 
GAGAAGACAG TTCTGAAGCG GGTGCTGAAG GAGCTCTGGA AGCTGGTGAT GAACACAATG 
GAGAAGACCA TCGTCCTGCC ACCGCTCACT GACCAGACGA TGATTGGCAC CCTCTTGAGA 
AAACATGGCA AGGGCCTAGA AAAGGGCAGG GTGAAACTGC CAAGCCACTC AGACGGGACA 
CAAATGATCT TCAATGCTGC CAAGGAGCTG GGCCAGCTGT CCAAACTTAA GGATCACATG 
GTGCGGGAAG AAGCCAAGAG CTTGACCCCG AAGCAGTGTG CTGTTGTTGA ACTGGCCCTG 
GACACCATCA AGCAATACTT CCACGCGGGG GGCGTTGGCC TCAAGAAGAC CTTCCTGGAG 
AAGAGCCCGG ACCTCCAGTC CCTGCGCTAC GCCCTGTCGC TCTACACGCA AGCCACCGAC 
CTGCTCATCA AAACCTTCGT GCAGACGCAG TCAGCGCAGG TCCATGGTGG AAAAGGGACT 
AGGTTTACCC TTAGTGAAGA CGTTTGTCCT GAGATGGGCT CTGGTGTGGA GGACCCTGTA 
GGTGAAGTGT CCGTCCACGT GGAGCTGTTC ACACATCCAG GAACTGGGGA ACAGAAGGTC 
ACAGTGAAGG TGGTGGCCGC CAATGACCTC AAGTGGCAGA CTTCTGGCAT CTTCCGCCCG 
TTCATCGAGG TCAACATCGT TGGACCTCAG CTTAGCGACA AGAAACGCAA GTTCGCCACC 
AAATCCAAAA ACAACAGCTG GGCGCCCAAG TATAACGAGA GCTTCCAGTT CTCCCTGAGC 
GCCGACGCGG GCCCCGAGTG CTACGAGCTG CAGGTGTGCG TGAAGGACTA CTGCTTTGCG 
CGCGAAGACC GCACGGTGGG GCTGGCGGTG CTGCAACTGC GAGAGCTGGC CCAGCGCGGG 
AGCGCCGCGT GCTGGCTTCC ACTCGGCCGC CGCATCCACA TGGACGACAC GGGGCTCACA 
GTGCTGCGCA TCCTGTCGCA GCGCAGCAAC GATGAGGTGG CCAAGGAATT CGTCAAGCTC 
AAGTCGGACA CGCGCTCAGC CGAGGAGGGC GGTGCCGCGC CTGCGCCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Unc13a ( XM_017312875.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168364.1 Mus musculus unc-13 homolog A (C. elegans) (Unc13a), transcript variant X2, mRNA.

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CloneID	OMu82526
Clone ID Related Accession (Same CDS sequence)	XM_017312875.1 , XM_017312875.2
Accession Version	XM_017312875.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein unc-13 homolog A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTGC TGTGCGTGGG AGTCAAAAAA GCCAAGTTTG ACGGTGCCCA AGAGAAGTTC 
AACACATACG TGACGCTGAA GGTGCAGAAC GTGAAGAGCA CTACCATAGC TGTACGTGGC 
AGCCAGCCCA GCTGGGAGCA GGACTTCATG TTTGAGATCA ACCGCCTGGA TCTAGGCCTG 
ACCGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGTAC TGTGTGGATC 
CCGCTGCGGA CCATCCGCCA GTCCAATGAG GAGGGCCCAG GAGAGTGGCT CACACTGGAC 
TCTCAGGCCA TCATGGCGGA CAGTGAGATC TGTGGGACCA AGGACCCCAC CTTCCATCGC 
ATCCTCCTGG ACGCTCATTT CGAGCTGCCG TTGGACATCC CTGAGGAGGA GGCACGCTAC 
TGGGCCAAGA AGCTGGAGCA GCTGAATGCC ATGCGTGACC AGGATGAGGT GAGTCACCTC 
CAGTACTCCT TCCAGGACCA GCAGGACAAG CCGCTGCCAG TGCCCAGCAG CCAGTGCTGC 
AACTGGAATT ACTTTGGCTG GGGAGAACAG AATGACGACC CCGACAGTGC GGTGGATGAC 
CGGGACAGTG ACTATAGGAG TGAGACCAGC AACAGCATCC CACCGCCCTA CTATACGACT 
TCGCAGCCCA ACGCCTCTGT GCACCAGTAC TCCGTGCGAC CGCCCCCTCT GGGGTCCCGG 
GAGTCCTACA GTGACTCCAT GCACAGCTAT GAGGAGTTCT CTGAGCCGCG GGCGCTCAGC 
CCCACAGGCA GCAGCCGCTA TGCTTCTTCC GGGGAGCTGA GCCAGGGAAG CTCACAGCTG 
AGCGAGGACT TCGACCCTGA TGAGCACAGC CTGCAGGGCT CGGAGCTGGA TGACGAGAGG 
GACCGCGATT CTTATCACTC CTGTCACAGC TCCGTGAGTT ACCACAAGGA CTCACCCCGC 
TGGGACCAGG ACGATGAGGA CCTGGAGGAC CTGGAGGACC TGGAAGATGA GGAGCTGCCC 
GAGGAGGAGG AGGAGTTGGA GGAGGAGGGG GAGGAGGAAT TGGAGGAAGA GGATTTGGAG 
GAGGAGGAGG AGGTGCCTGA TGACCTGGCC AGCTACACCC AGCAGGAGGA CACCACTGTG 
GCTGAGCCCA AAGAGTTCAA GCGGATCAGC TTCCCAACGG CTGCGCCTCA GAAGGATGAC 
AAGGTTTCAG CTGTGCCTAC AGAGGCCCCC GAGGTGGCCA AAGGCATCCC CAAAGCAGCC 
ACACCCGAGG AGAAGGCAGC TGCAGAGCGT GCGCAGGAAG CCGAGCCCCC CAAGTCTGAG 
GAGAGTTTCA GATCTCGAGA GGAGGAAGAG GGCCAGGAAG GGCAGGATGC CATGTCCAGA 
GCCAAAGCCA ACTGGTTGCG AGCCTTCAAC AAGGTGCGCA TGCAGCTGCA GGAGGCCCGA 
GGAGAAGGGG ATATGTCTAA GTCTCTATGG TTCAAAGGCG GTCCTGGTGG TGGCCTTATC 
ATCATTGACA GCATGCCAGA CATCAGGAAA CGGAAGCCCA TTCCCCTCGT GAGCGACCTG 
GCTATGTCAT TGGTGCAGTC TCGGAAGGCA GGCATCACCT CGGCCTTGGC CTCCAGCACG 
TTGAACAATG AAGAGCTGAA AAATCACGTT TACAAGAAGA CCCTGCAAGC CTTAATCTAC 
CCCATCTCCT GCACCACGCC GCACAATTTC GAGGTGTGGA CGGCCACCAC GCCCACCTAC 
TGCTATGAGT GCGAGGGGCT GCTGTGGGGC ATCGCGCGGC AGGGCATGCG ATGCACCGAG 
TGCGGCGTTA AGTGCCACGA GAAGTGCCAG GACCTGCTCA ATGCGGATTG CCTGCAGCGG 
GCGGCTGAGA AGAGTTCCAA GCATGGCGCT GAAGACCGCA CGCAGAACAT CATCATGGTG 
CTGAAGGACC GCATGAAGAT CCGCGAGCGC AACAAGCCTG AGATCTTCGA GCTTATCCAA 
GAGATCTTCG CGGTCACCAA GAGCGCACAC ACACAGCAGA TGAAAGCTGT CAAGCAGAGT 
GTGCTGGACG GCACATCCAA GTGGTCTGCC AAAATTAGCA TCACAGTGGT CTGTGCCCAG 
GGCTTGCAGG CCAAGGACAA GACAGGATCC AGTGACCCTT ATGTCACCGT CCAGGTTGGG 
AAGACCAAGA AAAGGACAAA AACCATCTAC GGGAACCTCA ACCCAGTGTG GGAAGAGAAT 
TTTCATTTTG AATGTCACAA CTCCTCTGAC CGGATCAAGG TTCGTGTTTG GGATGAAGAT 
GACGACATAA AATCCCGTGT GAAACAAAGG TTTAAGAGGG AGTCTGATGA CTTCCTAGGG 
CAGACAATCA TCGAGGTGCG GACGCTTAGC GGCGAGATGG ATGTGTGGTA CAATCTGGAC 
AAGAGAACGG ACAAGTCGGC GGTGTCGGGG GCCATCCGGC TTCACATCAG TGTGGAGATC 
AAAGGGGAGG AGAAGGTGGC GCCCTACCAT GTCCAGTACA CCTGTCTGCA TGAGAACCTG 
TTCCACTTTG TGACGGACGT GCAGAACAAT GGTGTGGTGA AGATTCCCGA TGCCAAGGGT 
GACGACGCCT GGAAGGTTTA CTACGATGAG ACGGCCCAGG AGATCGTGGA TGAGTTTGCC 
ATGCGCTATG GTGTTGAGTC CATCTACCAA GCCATGACCC ACTTTGCCTG CCTCTCCTCC 
AAGTATATGT GCCCTGGGGT ACCCGCTGTC ATGAGCACCC TGCTTGCCAA CATCAACGCC 
TACTACGCAC ACACCACCGC CTCCACCAAC GTGTCTGCCT CTGACCGCTT CGCTGCCTCT 
AATTTCGGGA AAGAGCGCTT TGTGAAACTT CTGGACCAGC TACACAATTC TCTGCGGATC 
GACCTGTCCA TGTACCGGAA CAACTTCCCA GCCAGCAGCC CTGAGCGGCT GCAGGATCTC 
AAGTCCACGG TGGACCTGCT CACCAGCATC ACCTTCTTCC GGATGAAGGT TCAGGAACTG 
CAGAGCCCGC CGCGTGCCAG CCAGGTAGTG AAAGACTGTG TGAAGGCCTG CCTCAACTCC 
ACCTATGAGT ACATCTTCAA CAACTGCCAT GAGCTCTATG GCCGGGAGTA CCAAACGGAT 
CCGGCCAAGA AGGGGGAGGT TCCCCCAGAG GAGCAAGGCC CTAGCATCAA GAACCTGGAT 
TTCTGGTCCA AGCTGATCAC CCTCATCGTG TCTATCATCG AGGAGGATAA GAATTCTTAC 
ACACCCTGCC TCAATCAGTT TCCCCAGGAG CTCAATGTGG GGAAGATCAG TGCTGAGGTG 
ATGTGGAGCC TATTTGCCCA AGACATGAAG TACGCCATGG AGGAACATGA CAAACACCGG 
CTGTGTAAGA GCGCAGATTA CATGAACCTG CACTTCAAGG TGAAGTGGCT GTACAATGAG 
TACGTGGCCG AGCTGCCCAC CTTCAAGGAC CGTGTGCCTG AGTACCCTGC GTGGTTTGAG 
CCCTTCGTCA TCCAGTGGTT GGATGAGAAT GAGGAGGTGT CCCGGGACTT CCTGCATGGT 
GCCCTCGAGC GGGACAAGAA GGACGGGTTC CAGCAGACCT CAGAGCACGC CCTGTTCTCT 
TGCTCAGTGG TGGACGTCTT CTCCCAGCTT AACCAGAGTT TCGAGATCAT CAAGAAGCTG 
GAGTGTCCTG ACCCCCAGAT CGTGGGCCAT TACATGCGGC GTTTTGCCAA GACCATTAGC 
AATGTGCTTC TCCAGTATGC AGACATCGTT TCCAAGGACT TCGCCTCCTA CTGCTCCAAG 
GAGAAGGAGA AACCCTGCAT CCTCATGAAC AACACACAGC AACTACGAGT GCAGCTGGAG 
AAGATGTTTG AGGCGATGGG CGGGAAGGAG CTGGATGCCG AGGCCAGTGG CACCCTGAAG 
GAGCTGCAGG TGAAACTCAA CAATGTCCTG GATGAACTCA GCCATGTGTT TGCCACCAGC 
TTCCAGCCAC ACATTGAGGA ATGCGTCAGA CAGATGGGTG ACATCCTGAG CCAGGTGAAG 
GGCACAGGCA ATGTACCTGC CAGTGCCTGC AGCAGCGTGG CCCAGGATGC AGACAATGTG 
CTGCAGCCCA TCATGGATCT TCTGGACAGC AACCTCACCC TGTTTGCCAA AATCTGTGAG 
AAGACAGTTC TGAAGCGGGT GCTGAAGGAG CTCTGGAAGC TGGTGATGAA CACAATGGAG 
AAGACCATCG TCCTGCCACC GCTCACTGAC CAGACGATGA TTGGCACCCT CTTGAGAAAA 
CATGGCAAGG GCCTAGAAAA GGGCAGGGTG AAACTGCCAA GCCACTCAGA CGGGACACAA 
ATGATCTTCA ATGCTGCCAA GGAGCTGGGC CAGCTGTCCA AACTTAAGGA TCACATGGTG 
CGGGAAGAAG CCAAGAGCTT GACCCCGAAG CAGTGTGCTG TTGTTGAACT GGCCCTGGAC 
ACCATCAAGC AATACTTCCA CGCGGGGGGC GTTGGCCTCA AGAAGACCTT CCTGGAGAAG 
AGCCCGGACC TCCAGTCCCT GCGCTACGCC CTGTCGCTCT ACACGCAAGC CACCGACCTG 
CTCATCAAAA CCTTCGTGCA GACGCAGTCA GCGCAGGTCC ATGGTGGAAA AGGGACTAGG 
TTTACCCTTA GTGAAGACGT TTGTCCTGAG ATGGGCTCTG GTGTGGAGGA CCCTGTAGGT 
GAAGTGTCCG TCCACGTGGA GCTGTTCACA CATCCAGGAA CTGGGGAACA GAAGGTCACA 
GTGAAGGTGG TGGCCGCCAA TGACCTCAAG TGGCAGACTT CTGGCATCTT CCGCCCGTTC 
ATCGAGGTCA ACATCGTTGG ACCTCAGCTT AGCGACAAGA AACGCAAGTT CGCCACCAAA 
TCCAAAAACA ACAGCTGGGC GCCCAAGTAT AACGAGAGCT TCCAGTTCTC CCTGAGCGCC 
GACGCGGGCC CCGAGTGCTA CGAGCTGCAG GTGTGCGTGA AGGACTACTG CTTTGCGCGC 
GAAGACCGCA CGGTGGGGCT GGCGGTGCTG CAACTGCGAG AGCTGGCCCA GCGCGGGAGC 
GCCGCGTGCT GGCTTCCACT CGGCCGCCGC ATCCACATGG ACGACACGGG GCTCACAGTG 
CTGCGCATCC TGTCGCAGCG CAGCAACGAT GAGGTGGCCA AGGAATTCGT CAAGCTCAAG 
TCGGACACGC GCTCAGCCGA GGAGGGCGGT GCCGCGCCTG CGCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168364.1
CDS	126..5333
Translation	MSLLCVGVKKAKFDGAQEKFNTYVTLKVQNVKSTTIAVRGSQPSWEQDFMFEINRLDLGLTVEVWNKGLIWDTMVGTVWIPLRTIRQSNEEGPGEWLTLDSQAIMADSEICGTKDPTFHRILLDAHFELPLDIPEEEARYWAKKLEQLNAMRDQDEVSHLQYSFQDQQDKPLPVPSSQCCNWNYFGWGEQNDDPDSAVDDRDSDYRSETSNSIPPPYYTTSQPNASVHQYSVRPPPLGSRESYSDSMHSYEEFSEPRALSPTGSSRYASSGELSQGSSQLSEDFDPDEHSLQGSELDDERDRDSYHSCHSSVSYHKDSPRWDQDDEDLEDLEDLEDEELPEEEEELEEEGEEELEEEDLEEEEEVPDDLASYTQQEDTTVAEPKEFKRISFPTAAPQKDDKVSAVPTEAPEVAKGIPKAATPEEKAAAERAQEAEPPKSEESFRSREEEEGQEGQDAMSRAKANWLRAFNKVRMQLQEARGEGDMSKSLWFKGGPGGGLIIIDSMPDIRKRKPIPLVSDLAMSLVQSRKAGITSALASSTLNNEELKNHVYKKTLQALIYPISCTTPHNFEVWTATTPTYCYECEGLLWGIARQGMRCTECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMVLKDRMKIRERNKPEIFELIQEIFAVTKSAHTQQMKAVKQSVLDGTSKWSAKISITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIYGNLNPVWEENFHFECHNSSDRIKVRVWDEDDDIKSRVKQRFKRESDDFLGQTIIEVRTLSGEMDVWYNLDKRTDKSAVSGAIRLHISVEIKGEEKVAPYHVQYTCLHENLFHFVTDVQNNGVVKIPDAKGDDAWKVYYDETAQEIVDEFAMRYGVESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSMYRNNFPASSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHELYGREYQTDPAKKGEVPPEEQGPSIKNLDFWSKLITLIVSIIEEDKNSYTPCLNQFPQELNVGKISAEVMWSLFAQDMKYAMEEHDKHRLCKSADYMNLHFKVKWLYNEYVAELPTFKDRVPEYPAWFEPFVIQWLDENEEVSRDFLHGALERDKKDGFQQTSEHALFSCSVVDVFSQLNQSFEIIKKLECPDPQIVGHYMRRFAKTISNVLLQYADIVSKDFASYCSKEKEKPCILMNNTQQLRVQLEKMFEAMGGKELDAEASGTLKELQVKLNNVLDELSHVFATSFQPHIEECVRQMGDILSQVKGTGNVPASACSSVAQDADNVLQPIMDLLDSNLTLFAKICEKTVLKRVLKELWKLVMNTMEKTIVLPPLTDQTMIGTLLRKHGKGLEKGRVKLPSHSDGTQMIFNAAKELGQLSKLKDHMVREEAKSLTPKQCAVVELALDTIKQYFHAGGVGLKKTFLEKSPDLQSLRYALSLYTQATDLLIKTFVQTQSAQVHGGKGTRFTLSEDVCPEMGSGVEDPVGEVSVHVELFTHPGTGEQKVTVKVVAANDLKWQTSGIFRPFIEVNIVGPQLSDKKRKFATKSKNNSWAPKYNESFQFSLSADAGPECYELQVCVKDYCFAREDRTVGLAVLQLRELAQRGSAACWLPLGRRIHMDDTGLTVLRILSQRSNDEVAKEFVKLKSDTRSAEEGGAAPAP

Target ORF information:

RefSeq Version	XM_017312875.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus unc-13 homolog A (C. elegans) (Unc13a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312875.1

ORF Insert Sequence:

ATGTCTCTGC TGTGCGTGGG AGTCAAAAAA GCCAAGTTTG ACGGTGCCCA AGAGAAGTTC 
AACACATACG TGACGCTGAA GGTGCAGAAC GTGAAGAGCA CTACCATAGC TGTACGTGGC 
AGCCAGCCCA GCTGGGAGCA GGACTTCATG TTTGAGATCA ACCGCCTGGA TCTAGGCCTG 
ACCGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGTAC TGTGTGGATC 
CCGCTGCGGA CCATCCGCCA GTCCAATGAG GAGGGCCCAG GAGAGTGGCT CACACTGGAC 
TCTCAGGCCA TCATGGCGGA CAGTGAGATC TGTGGGACCA AGGACCCCAC CTTCCATCGC 
ATCCTCCTGG ACGCTCATTT CGAGCTGCCG TTGGACATCC CTGAGGAGGA GGCACGCTAC 
TGGGCCAAGA AGCTGGAGCA GCTGAATGCC ATGCGTGACC AGGATGAGGT GAGTCACCTC 
CAGTACTCCT TCCAGGACCA GCAGGACAAG CCGCTGCCAG TGCCCAGCAG CCAGTGCTGC 
AACTGGAATT ACTTTGGCTG GGGAGAACAG AATGACGACC CCGACAGTGC GGTGGATGAC 
CGGGACAGTG ACTATAGGAG TGAGACCAGC AACAGCATCC CACCGCCCTA CTATACGACT 
TCGCAGCCCA ACGCCTCTGT GCACCAGTAC TCCGTGCGAC CGCCCCCTCT GGGGTCCCGG 
GAGTCCTACA GTGACTCCAT GCACAGCTAT GAGGAGTTCT CTGAGCCGCG GGCGCTCAGC 
CCCACAGGCA GCAGCCGCTA TGCTTCTTCC GGGGAGCTGA GCCAGGGAAG CTCACAGCTG 
AGCGAGGACT TCGACCCTGA TGAGCACAGC CTGCAGGGCT CGGAGCTGGA TGACGAGAGG 
GACCGCGATT CTTATCACTC CTGTCACAGC TCCGTGAGTT ACCACAAGGA CTCACCCCGC 
TGGGACCAGG ACGATGAGGA CCTGGAGGAC CTGGAGGACC TGGAAGATGA GGAGCTGCCC 
GAGGAGGAGG AGGAGTTGGA GGAGGAGGGG GAGGAGGAAT TGGAGGAAGA GGATTTGGAG 
GAGGAGGAGG AGGTGCCTGA TGACCTGGCC AGCTACACCC AGCAGGAGGA CACCACTGTG 
GCTGAGCCCA AAGAGTTCAA GCGGATCAGC TTCCCAACGG CTGCGCCTCA GAAGGATGAC 
AAGGTTTCAG CTGTGCCTAC AGAGGCCCCC GAGGTGGCCA AAGGCATCCC CAAAGCAGCC 
ACACCCGAGG AGAAGGCAGC TGCAGAGCGT GCGCAGGAAG CCGAGCCCCC CAAGTCTGAG 
GAGAGTTTCA GATCTCGAGA GGAGGAAGAG GGCCAGGAAG GGCAGGATGC CATGTCCAGA 
GCCAAAGCCA ACTGGTTGCG AGCCTTCAAC AAGGTGCGCA TGCAGCTGCA GGAGGCCCGA 
GGAGAAGGGG ATATGTCTAA GTCTCTATGG TTCAAAGGCG GTCCTGGTGG TGGCCTTATC 
ATCATTGACA GCATGCCAGA CATCAGGAAA CGGAAGCCCA TTCCCCTCGT GAGCGACCTG 
GCTATGTCAT TGGTGCAGTC TCGGAAGGCA GGCATCACCT CGGCCTTGGC CTCCAGCACG 
TTGAACAATG AAGAGCTGAA AAATCACGTT TACAAGAAGA CCCTGCAAGC CTTAATCTAC 
CCCATCTCCT GCACCACGCC GCACAATTTC GAGGTGTGGA CGGCCACCAC GCCCACCTAC 
TGCTATGAGT GCGAGGGGCT GCTGTGGGGC ATCGCGCGGC AGGGCATGCG ATGCACCGAG 
TGCGGCGTTA AGTGCCACGA GAAGTGCCAG GACCTGCTCA ATGCGGATTG CCTGCAGCGG 
GCGGCTGAGA AGAGTTCCAA GCATGGCGCT GAAGACCGCA CGCAGAACAT CATCATGGTG 
CTGAAGGACC GCATGAAGAT CCGCGAGCGC AACAAGCCTG AGATCTTCGA GCTTATCCAA 
GAGATCTTCG CGGTCACCAA GAGCGCACAC ACACAGCAGA TGAAAGCTGT CAAGCAGAGT 
GTGCTGGACG GCACATCCAA GTGGTCTGCC AAAATTAGCA TCACAGTGGT CTGTGCCCAG 
GGCTTGCAGG CCAAGGACAA GACAGGATCC AGTGACCCTT ATGTCACCGT CCAGGTTGGG 
AAGACCAAGA AAAGGACAAA AACCATCTAC GGGAACCTCA ACCCAGTGTG GGAAGAGAAT 
TTTCATTTTG AATGTCACAA CTCCTCTGAC CGGATCAAGG TTCGTGTTTG GGATGAAGAT 
GACGACATAA AATCCCGTGT GAAACAAAGG TTTAAGAGGG AGTCTGATGA CTTCCTAGGG 
CAGACAATCA TCGAGGTGCG GACGCTTAGC GGCGAGATGG ATGTGTGGTA CAATCTGGAC 
AAGAGAACGG ACAAGTCGGC GGTGTCGGGG GCCATCCGGC TTCACATCAG TGTGGAGATC 
AAAGGGGAGG AGAAGGTGGC GCCCTACCAT GTCCAGTACA CCTGTCTGCA TGAGAACCTG 
TTCCACTTTG TGACGGACGT GCAGAACAAT GGTGTGGTGA AGATTCCCGA TGCCAAGGGT 
GACGACGCCT GGAAGGTTTA CTACGATGAG ACGGCCCAGG AGATCGTGGA TGAGTTTGCC 
ATGCGCTATG GTGTTGAGTC CATCTACCAA GCCATGACCC ACTTTGCCTG CCTCTCCTCC 
AAGTATATGT GCCCTGGGGT ACCCGCTGTC ATGAGCACCC TGCTTGCCAA CATCAACGCC 
TACTACGCAC ACACCACCGC CTCCACCAAC GTGTCTGCCT CTGACCGCTT CGCTGCCTCT 
AATTTCGGGA AAGAGCGCTT TGTGAAACTT CTGGACCAGC TACACAATTC TCTGCGGATC 
GACCTGTCCA TGTACCGGAA CAACTTCCCA GCCAGCAGCC CTGAGCGGCT GCAGGATCTC 
AAGTCCACGG TGGACCTGCT CACCAGCATC ACCTTCTTCC GGATGAAGGT TCAGGAACTG 
CAGAGCCCGC CGCGTGCCAG CCAGGTAGTG AAAGACTGTG TGAAGGCCTG CCTCAACTCC 
ACCTATGAGT ACATCTTCAA CAACTGCCAT GAGCTCTATG GCCGGGAGTA CCAAACGGAT 
CCGGCCAAGA AGGGGGAGGT TCCCCCAGAG GAGCAAGGCC CTAGCATCAA GAACCTGGAT 
TTCTGGTCCA AGCTGATCAC CCTCATCGTG TCTATCATCG AGGAGGATAA GAATTCTTAC 
ACACCCTGCC TCAATCAGTT TCCCCAGGAG CTCAATGTGG GGAAGATCAG TGCTGAGGTG 
ATGTGGAGCC TATTTGCCCA AGACATGAAG TACGCCATGG AGGAACATGA CAAACACCGG 
CTGTGTAAGA GCGCAGATTA CATGAACCTG CACTTCAAGG TGAAGTGGCT GTACAATGAG 
TACGTGGCCG AGCTGCCCAC CTTCAAGGAC CGTGTGCCTG AGTACCCTGC GTGGTTTGAG 
CCCTTCGTCA TCCAGTGGTT GGATGAGAAT GAGGAGGTGT CCCGGGACTT CCTGCATGGT 
GCCCTCGAGC GGGACAAGAA GGACGGGTTC CAGCAGACCT CAGAGCACGC CCTGTTCTCT 
TGCTCAGTGG TGGACGTCTT CTCCCAGCTT AACCAGAGTT TCGAGATCAT CAAGAAGCTG 
GAGTGTCCTG ACCCCCAGAT CGTGGGCCAT TACATGCGGC GTTTTGCCAA GACCATTAGC 
AATGTGCTTC TCCAGTATGC AGACATCGTT TCCAAGGACT TCGCCTCCTA CTGCTCCAAG 
GAGAAGGAGA AACCCTGCAT CCTCATGAAC AACACACAGC AACTACGAGT GCAGCTGGAG 
AAGATGTTTG AGGCGATGGG CGGGAAGGAG CTGGATGCCG AGGCCAGTGG CACCCTGAAG 
GAGCTGCAGG TGAAACTCAA CAATGTCCTG GATGAACTCA GCCATGTGTT TGCCACCAGC 
TTCCAGCCAC ACATTGAGGA ATGCGTCAGA CAGATGGGTG ACATCCTGAG CCAGGTGAAG 
GGCACAGGCA ATGTACCTGC CAGTGCCTGC AGCAGCGTGG CCCAGGATGC AGACAATGTG 
CTGCAGCCCA TCATGGATCT TCTGGACAGC AACCTCACCC TGTTTGCCAA AATCTGTGAG 
AAGACAGTTC TGAAGCGGGT GCTGAAGGAG CTCTGGAAGC TGGTGATGAA CACAATGGAG 
AAGACCATCG TCCTGCCACC GCTCACTGAC CAGACGATGA TTGGCACCCT CTTGAGAAAA 
CATGGCAAGG GCCTAGAAAA GGGCAGGGTG AAACTGCCAA GCCACTCAGA CGGGACACAA 
ATGATCTTCA ATGCTGCCAA GGAGCTGGGC CAGCTGTCCA AACTTAAGGA TCACATGGTG 
CGGGAAGAAG CCAAGAGCTT GACCCCGAAG CAGTGTGCTG TTGTTGAACT GGCCCTGGAC 
ACCATCAAGC AATACTTCCA CGCGGGGGGC GTTGGCCTCA AGAAGACCTT CCTGGAGAAG 
AGCCCGGACC TCCAGTCCCT GCGCTACGCC CTGTCGCTCT ACACGCAAGC CACCGACCTG 
CTCATCAAAA CCTTCGTGCA GACGCAGTCA GCGCAGGTCC ATGGTGGAAA AGGGACTAGG 
TTTACCCTTA GTGAAGACGT TTGTCCTGAG ATGGGCTCTG GTGTGGAGGA CCCTGTAGGT 
GAAGTGTCCG TCCACGTGGA GCTGTTCACA CATCCAGGAA CTGGGGAACA GAAGGTCACA 
GTGAAGGTGG TGGCCGCCAA TGACCTCAAG TGGCAGACTT CTGGCATCTT CCGCCCGTTC 
ATCGAGGTCA ACATCGTTGG ACCTCAGCTT AGCGACAAGA AACGCAAGTT CGCCACCAAA 
TCCAAAAACA ACAGCTGGGC GCCCAAGTAT AACGAGAGCT TCCAGTTCTC CCTGAGCGCC 
GACGCGGGCC CCGAGTGCTA CGAGCTGCAG GTGTGCGTGA AGGACTACTG CTTTGCGCGC 
GAAGACCGCA CGGTGGGGCT GGCGGTGCTG CAACTGCGAG AGCTGGCCCA GCGCGGGAGC 
GCCGCGTGCT GGCTTCCACT CGGCCGCCGC ATCCACATGG ACGACACGGG GCTCACAGTG 
CTGCGCATCC TGTCGCAGCG CAGCAACGAT GAGGTGGCCA AGGAATTCGT CAAGCTCAAG 
TCGGACACGC GCTCAGCCGA GGAGGGCGGT GCCGCGCCTG CGCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Unc13a ( XM_017312877.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168366.1 Mus musculus unc-13 homolog A (C. elegans) (Unc13a), transcript variant X5, mRNA.

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CloneID	OMu82527
Clone ID Related Accession (Same CDS sequence)	XM_017312877.1 , XM_017312877.2
Accession Version	XM_017312877.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5154bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein unc-13 homolog A isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTGC TGTGCGTGGG AGTCAAAAAA GCCAAGTTTG ACGGTGCCCA AGAGAAGTTC 
AACACATACG TGACGCTGAA GGTGCAGAAC GTGAAGAGCA CTACCATAGC TGTACGTGGC 
AGCCAGCCCA GCTGGGAGCA GGACTTCATG TTTGAGATCA ACCGCCTGGA TCTAGGCCTG 
ACCGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGTAC TGTGTGGATC 
CCGCTGCGGA CCATCCGCCA GTCCAATGAG GAGGGCCCAG GAGAGTGGCT CACACTGGAC 
TCTCAGGCCA TCATGGCGGA CAGTGAGATC TGTGGGACCA AGGACCCCAC CTTCCATCGC 
ATCCTCCTGG ACGCTCATTT CGAGCTGCCG TTGGACATCC CTGAGGAGGA GGCACGCTAC 
TGGGCCAAGA AGCTGGAGCA GCTGAATGCC ATGCGTGACC AGGATGAGGT GAGTCACCTC 
CAGTACTCCT TCCAGGACCA GCAGGACAAG CCGCTGCCAG TGCCCAGCAG CCAGTGCTGC 
AACTGGAATT ACTTTGGCTG GGGAGAACAG AATGACGACC CCGACAGTGC GGTGGATGAC 
CGGGACAGTG ACTATAGGAG TGAGACCAGC AACAGCATCC CACCGCCCTA CTATACGACT 
TCGCAGCCCA ACGCCTCTGT GCACCAGTAC TCCGTGCGAC CGCCCCCTCT GGGGTCCCGG 
GAGTCCTACA GTGACTCCAT GCACAGCTAT GAGGAGTTCT CTGAGCCGCG GGCGCTCAGC 
CCCACAGGCA GCAGCCGCTA TGCTTCTTCC GGGGAGCTGA GCCAGGGAAG CTCACAGCTG 
AGCGAGGACT TCGACCCTGA TGAGCACAGC CTGCAGGGCT CGGAGCTGGA TGACGAGAGG 
GACCGCGATT CTTATCACTC CTGTCACAGC TCCGTGAGTT ACCACAAGGA CTCACCCCGC 
TGGGACCAGG ACGATGAGGA CCTGGAGGAC CTGGAGGACC TGGAAGATGA GGAGCTGCCC 
GAGGAGGAGG AGGAGTTGGA GGAGGAGGGG GAGGAGGAAT TGGAGGAAGA GGATTTGGAG 
GAGGAGGAGG AGGTGCCTGA TGACCTGGCC AGCTACACCC AGCAGGAGGA CACCACTGTG 
GCTGAGCCCA AAGAGTTCAA GCGGATCAGC TTCCCAACGG CTGCGCCTCA GAAGGATGAC 
AAGGTTTCAG CTGTGCCTAC AGAGGCCCCC GAGGTGGCCA AAGGCATCCC CAAAGCAGCC 
ACACCCGAGG AGAAGGCAGC TGCAGAGCGT GCGCAGGAAG CCGAGCCCCC CAAGTCTGAG 
GAGAGTTTCA GATCTCGAGA GGAGGAAGAG GGCCAGGAAG GGCAGGATGC CATGTCCAGA 
GCCAAAGCCA ACTGGTTGCG AGCCTTCAAC AAGGTGCGCA TGCAGCTGCA GGAGGCCCGA 
GGAGAAGGGG ATATGTCTAA GTCTCTATGG TTCAAAGGCG GTCCTGGTGG TGGCCTTATC 
ATCATTGACA GCATGCCAGA CATCAGGAAA CGGAAGCCCA TTCCCCTCGT GAGCGACCTG 
GCTATGTCAT TGGTGCAGTC TCGGAAGGCA GGCATCACCT CGGCCTTGGC CTCCAGCACG 
TTGAACAATG AAGAGCTGAA AAATCACGTT TACAAGAAGA CCCTGCAAGC CTTAATCTAC 
CCCATCTCCT GCACCACGCC GCACAATTTC GAGGTGTGGA CGGCCACCAC GCCCACCTAC 
TGCTATGAGT GCGAGGGGCT GCTGTGGGGC ATCGCGCGGC AGGGCATGCG ATGCACCGAG 
TGCGGCGTTA AGTGCCACGA GAAGTGCCAG GACCTGCTCA ATGCGGATTG CCTGCAGCGG 
GCGGCTGAGA AGAGTTCCAA GCATGGCGCT GAAGACCGCA CGCAGAACAT CATCATGGTG 
CTGAAGGACC GCATGAAGAT CCGCGAGCGC AACAAGCCTG AGATCTTCGA GCTTATCCAA 
GAGATCTTCG CGGTCACCAA GAGCGCACAC ACACAGCAGA TGAAAGCTGT CAAGCAGAGT 
GTGCTGGACG GCACATCCAA GTGGTCTGCC AAAATTAGCA TCACAGTGGT CTGTGCCCAG 
GGCTTGCAGG CCAAGGACAA GACAGGATCC AGTGACCCTT ATGTCACCGT CCAGGTTGGG 
AAGACCAAGA AAAGGACAAA AACCATCTAC GGGAACCTCA ACCCAGTGTG GGAAGAGAAT 
TTTCATTTTG AATGTCACAA CTCCTCTGAC CGGATCAAGG TTCGTGTTTG GGATGAAGAT 
GACGACATAA AATCCCGTGT GAAACAAAGG TTTAAGAGGG AGTCTGATGA CTTCCTAGGG 
CAGACAATCA TCGAGGTGCG GACGCTTAGC GGCGAGATGG ATGTGTGGTA CAATCTGGAC 
AAGAGAACGG ACAAGTCGGC GGTGTCGGGG GCCATCCGGC TTCACATCAG TGTGGAGATC 
AAAGGGGAGG AGAAGGTGGC GCCCTACCAT GTCCAGTACA CCTGTCTGCA TGAGAACCTG 
TTCCACTTTG TGACGGACGT GCAGAACAAT GGTGTGGTGA AGATTCCCGA TGCCAAGGGT 
GACGACGCCT GGAAGGTTTA CTACGATGAG ACGGCCCAGG AGATCGTGGA TGAGTTTGCC 
ATGCGCTATG GTGTTGAGTC CATCTACCAA GCCATGACCC ACTTTGCCTG CCTCTCCTCC 
AAGTATATGT GCCCTGGGGT ACCCGCTGTC ATGAGCACCC TGCTTGCCAA CATCAACGCC 
TACTACGCAC ACACCACCGC CTCCACCAAC GTGTCTGCCT CTGACCGCTT CGCTGCCTCT 
AATTTCGGGA AAGAGCGCTT TGTGAAACTT CTGGACCAGC TACACAATTC TCTGCGGATC 
GACCTGTCCA TGTACCGGAA CAACTTCCCA GCCAGCAGCC CTGAGCGGCT GCAGGATCTC 
AAGTCCACGG TGGACCTGCT CACCAGCATC ACCTTCTTCC GGATGAAGGT TCAGGAACTG 
CAGAGCCCGC CGCGTGCCAG CCAGGTAGTG AAAGACTGTG TGAAGGCCTG CCTCAACTCC 
ACCTATGAGT ACATCTTCAA CAACTGCCAT GAGCTCTATG GCCGGGAGTA CCAAACGGAT 
CCGGCCAAGA AGGGGGAGGT TCCCCCAGAG GAGCAAGGCC CTAGCATCAA GAACCTGGAT 
TTCTGGTCCA AGCTGATCAC CCTCATCGTG TCTATCATCG AGGAGGATAA GAATTCTTAC 
ACACCCTGCC TCAATCAGTT TCCCCAGGAG CTCAATGTGG GGAAGATCAG TGCTGAGGTG 
ATGTGGAGCC TATTTGCCCA AGACATGAAG TACGCCATGG AGGAACATGA CAAACACCGG 
CTGTGTAAGA GCGCAGATTA CATGAACCTG CACTTCAAGG TGAAGTGGCT GTACAATGAG 
TACGTGGCCG AGCTGCCCAC CTTCAAGGAC CGTGTGCCTG AGTACCCTGC GTGGTTTGAG 
CCCTTCGTCA TCCAGTGGTT GGATGAGAAT GAGGAGGTGT CCCGGGACTT CCTGCATGGT 
GCCCTCGAGC GGGACAAGAA GGACGGGTTC CAGCAGACCT CAGAGCACGC CCTGTTCTCT 
TGCTCAGTGG TGGACGTCTT CTCCCAGCTT AACCAGAGTT TCGAGATCAT CAAGAAGCTG 
GAGTGTCCTG ACCCCCAGAT CGTGGGCCAT TACATGCGGC GTTTTGCCAA GACCATTAGC 
AATGTGCTTC TCCAGTATGC AGACATCGTT TCCAAGGACT TCGCCTCCTA CTGCTCCAAG 
GAGAAGGAGA AAGTGCCCTG CATCCTCATG AACAACACAC AGCAACTACG AGTGCAGCTG 
GAGAAGATGT TTGAGGCGAT GGGCGGGAAG GAGCTGGATG CCGAGGCCAG TGGCACCCTG 
AAGGAGCTGC AGGTGAAACT CAACAATGTC CTGGATGAAC TCAGCCATGT GTTTGCCACC 
AGCTTCCAGC CACACATTGA GGAATGCGTC AGACAGATGG GTGACATCCT GAGCCAGGTG 
AAGGGCACAG GCAATGTACC TGCCAGTGCC TGCAGCAGCG TGGCCCAGGA TGCAGACAAT 
GTGCTGCAGC CCATCATGGA TCTTCTGGAC AGCAACCTCA CCCTGTTTGC CAAAATCTGT 
GAGAAGACAG TTCTGAAGCG GGTGCTGAAG GAGCTCTGGA AGCTGGTGAT GAACACAATG 
GAGAAGACCA TCGTCCTGCC ACCGCTCACT GACCAGACGA TGATTGGCAC CCTCTTGAGA 
AAACATGGCA AGGGCCTAGA AAAGGGCAGG GTGAAACTGC CAAGCCACTC AGACGGGACA 
CAAATGATCT TCAATGCTGC CAAGGAGCTG GGCCAGCTGT CCAAACTTAA GGATCACATG 
GTGCGGGAAG AAGCCAAGAG CTTGACCCCG AAGCAGTGTG CTGTTGTTGA ACTGGCCCTG 
GACACCATCA AGCAATACTT CCACGCGGGG GGCGTTGGCC TCAAGAAGAC CTTCCTGGAG 
AAGAGCCCGG ACCTCCAGTC CCTGCGCTAC GCCCTGTCGC TCTACACGCA AGCCACCGAC 
CTGCTCATCA AAACCTTCGT GCAGACGCAG TCAGCGCAGG GCTCTGGTGT GGAGGACCCT 
GTAGGTGAAG TGTCCGTCCA CGTGGAGCTG TTCACACATC CAGGAACTGG GGAACAGAAG 
GTCACAGTGA AGGTGGTGGC CGCCAATGAC CTCAAGTGGC AGACTTCTGG CATCTTCCGC 
CCGTTCATCG AGGTCAACAT CGTTGGACCT CAGCTTAGCG ACAAGAAACG CAAGTTCGCC 
ACCAAATCCA AAAACAACAG CTGGGCGCCC AAGTATAACG AGAGCTTCCA GTTCTCCCTG 
AGCGCCGACG CGGGCCCCGA GTGCTACGAG CTGCAGGTGT GCGTGAAGGA CTACTGCTTT 
GCGCGCGAAG ACCGCACGGT GGGGCTGGCG GTGCTGCAAC TGCGAGAGCT GGCCCAGCGC 
GGGAGCGCCG CGTGCTGGCT TCCACTCGGC CGCCGCATCC ACATGGACGA CACGGGGCTC 
ACAGTGCTGC GCATCCTGTC GCAGCGCAGC AACGATGAGG TGGCCAAGGA ATTCGTCAAG 
CTCAAGTCGG ACACGCGCTC AGCCGAGGAG GGCGGTGCCG CGCCTGCGCC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168366.1
CDS	125..5278
Translation	MSLLCVGVKKAKFDGAQEKFNTYVTLKVQNVKSTTIAVRGSQPSWEQDFMFEINRLDLGLTVEVWNKGLIWDTMVGTVWIPLRTIRQSNEEGPGEWLTLDSQAIMADSEICGTKDPTFHRILLDAHFELPLDIPEEEARYWAKKLEQLNAMRDQDEVSHLQYSFQDQQDKPLPVPSSQCCNWNYFGWGEQNDDPDSAVDDRDSDYRSETSNSIPPPYYTTSQPNASVHQYSVRPPPLGSRESYSDSMHSYEEFSEPRALSPTGSSRYASSGELSQGSSQLSEDFDPDEHSLQGSELDDERDRDSYHSCHSSVSYHKDSPRWDQDDEDLEDLEDLEDEELPEEEEELEEEGEEELEEEDLEEEEEVPDDLASYTQQEDTTVAEPKEFKRISFPTAAPQKDDKVSAVPTEAPEVAKGIPKAATPEEKAAAERAQEAEPPKSEESFRSREEEEGQEGQDAMSRAKANWLRAFNKVRMQLQEARGEGDMSKSLWFKGGPGGGLIIIDSMPDIRKRKPIPLVSDLAMSLVQSRKAGITSALASSTLNNEELKNHVYKKTLQALIYPISCTTPHNFEVWTATTPTYCYECEGLLWGIARQGMRCTECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMVLKDRMKIRERNKPEIFELIQEIFAVTKSAHTQQMKAVKQSVLDGTSKWSAKISITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIYGNLNPVWEENFHFECHNSSDRIKVRVWDEDDDIKSRVKQRFKRESDDFLGQTIIEVRTLSGEMDVWYNLDKRTDKSAVSGAIRLHISVEIKGEEKVAPYHVQYTCLHENLFHFVTDVQNNGVVKIPDAKGDDAWKVYYDETAQEIVDEFAMRYGVESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSMYRNNFPASSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHELYGREYQTDPAKKGEVPPEEQGPSIKNLDFWSKLITLIVSIIEEDKNSYTPCLNQFPQELNVGKISAEVMWSLFAQDMKYAMEEHDKHRLCKSADYMNLHFKVKWLYNEYVAELPTFKDRVPEYPAWFEPFVIQWLDENEEVSRDFLHGALERDKKDGFQQTSEHALFSCSVVDVFSQLNQSFEIIKKLECPDPQIVGHYMRRFAKTISNVLLQYADIVSKDFASYCSKEKEKVPCILMNNTQQLRVQLEKMFEAMGGKELDAEASGTLKELQVKLNNVLDELSHVFATSFQPHIEECVRQMGDILSQVKGTGNVPASACSSVAQDADNVLQPIMDLLDSNLTLFAKICEKTVLKRVLKELWKLVMNTMEKTIVLPPLTDQTMIGTLLRKHGKGLEKGRVKLPSHSDGTQMIFNAAKELGQLSKLKDHMVREEAKSLTPKQCAVVELALDTIKQYFHAGGVGLKKTFLEKSPDLQSLRYALSLYTQATDLLIKTFVQTQSAQGSGVEDPVGEVSVHVELFTHPGTGEQKVTVKVVAANDLKWQTSGIFRPFIEVNIVGPQLSDKKRKFATKSKNNSWAPKYNESFQFSLSADAGPECYELQVCVKDYCFAREDRTVGLAVLQLRELAQRGSAACWLPLGRRIHMDDTGLTVLRILSQRSNDEVAKEFVKLKSDTRSAEEGGAAPAP

Target ORF information:

RefSeq Version	XM_017312877.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus unc-13 homolog A (C. elegans) (Unc13a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312877.1

ORF Insert Sequence:

ATGTCTCTGC TGTGCGTGGG AGTCAAAAAA GCCAAGTTTG ACGGTGCCCA AGAGAAGTTC 
AACACATACG TGACGCTGAA GGTGCAGAAC GTGAAGAGCA CTACCATAGC TGTACGTGGC 
AGCCAGCCCA GCTGGGAGCA GGACTTCATG TTTGAGATCA ACCGCCTGGA TCTAGGCCTG 
ACCGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGTAC TGTGTGGATC 
CCGCTGCGGA CCATCCGCCA GTCCAATGAG GAGGGCCCAG GAGAGTGGCT CACACTGGAC 
TCTCAGGCCA TCATGGCGGA CAGTGAGATC TGTGGGACCA AGGACCCCAC CTTCCATCGC 
ATCCTCCTGG ACGCTCATTT CGAGCTGCCG TTGGACATCC CTGAGGAGGA GGCACGCTAC 
TGGGCCAAGA AGCTGGAGCA GCTGAATGCC ATGCGTGACC AGGATGAGGT GAGTCACCTC 
CAGTACTCCT TCCAGGACCA GCAGGACAAG CCGCTGCCAG TGCCCAGCAG CCAGTGCTGC 
AACTGGAATT ACTTTGGCTG GGGAGAACAG AATGACGACC CCGACAGTGC GGTGGATGAC 
CGGGACAGTG ACTATAGGAG TGAGACCAGC AACAGCATCC CACCGCCCTA CTATACGACT 
TCGCAGCCCA ACGCCTCTGT GCACCAGTAC TCCGTGCGAC CGCCCCCTCT GGGGTCCCGG 
GAGTCCTACA GTGACTCCAT GCACAGCTAT GAGGAGTTCT CTGAGCCGCG GGCGCTCAGC 
CCCACAGGCA GCAGCCGCTA TGCTTCTTCC GGGGAGCTGA GCCAGGGAAG CTCACAGCTG 
AGCGAGGACT TCGACCCTGA TGAGCACAGC CTGCAGGGCT CGGAGCTGGA TGACGAGAGG 
GACCGCGATT CTTATCACTC CTGTCACAGC TCCGTGAGTT ACCACAAGGA CTCACCCCGC 
TGGGACCAGG ACGATGAGGA CCTGGAGGAC CTGGAGGACC TGGAAGATGA GGAGCTGCCC 
GAGGAGGAGG AGGAGTTGGA GGAGGAGGGG GAGGAGGAAT TGGAGGAAGA GGATTTGGAG 
GAGGAGGAGG AGGTGCCTGA TGACCTGGCC AGCTACACCC AGCAGGAGGA CACCACTGTG 
GCTGAGCCCA AAGAGTTCAA GCGGATCAGC TTCCCAACGG CTGCGCCTCA GAAGGATGAC 
AAGGTTTCAG CTGTGCCTAC AGAGGCCCCC GAGGTGGCCA AAGGCATCCC CAAAGCAGCC 
ACACCCGAGG AGAAGGCAGC TGCAGAGCGT GCGCAGGAAG CCGAGCCCCC CAAGTCTGAG 
GAGAGTTTCA GATCTCGAGA GGAGGAAGAG GGCCAGGAAG GGCAGGATGC CATGTCCAGA 
GCCAAAGCCA ACTGGTTGCG AGCCTTCAAC AAGGTGCGCA TGCAGCTGCA GGAGGCCCGA 
GGAGAAGGGG ATATGTCTAA GTCTCTATGG TTCAAAGGCG GTCCTGGTGG TGGCCTTATC 
ATCATTGACA GCATGCCAGA CATCAGGAAA CGGAAGCCCA TTCCCCTCGT GAGCGACCTG 
GCTATGTCAT TGGTGCAGTC TCGGAAGGCA GGCATCACCT CGGCCTTGGC CTCCAGCACG 
TTGAACAATG AAGAGCTGAA AAATCACGTT TACAAGAAGA CCCTGCAAGC CTTAATCTAC 
CCCATCTCCT GCACCACGCC GCACAATTTC GAGGTGTGGA CGGCCACCAC GCCCACCTAC 
TGCTATGAGT GCGAGGGGCT GCTGTGGGGC ATCGCGCGGC AGGGCATGCG ATGCACCGAG 
TGCGGCGTTA AGTGCCACGA GAAGTGCCAG GACCTGCTCA ATGCGGATTG CCTGCAGCGG 
GCGGCTGAGA AGAGTTCCAA GCATGGCGCT GAAGACCGCA CGCAGAACAT CATCATGGTG 
CTGAAGGACC GCATGAAGAT CCGCGAGCGC AACAAGCCTG AGATCTTCGA GCTTATCCAA 
GAGATCTTCG CGGTCACCAA GAGCGCACAC ACACAGCAGA TGAAAGCTGT CAAGCAGAGT 
GTGCTGGACG GCACATCCAA GTGGTCTGCC AAAATTAGCA TCACAGTGGT CTGTGCCCAG 
GGCTTGCAGG CCAAGGACAA GACAGGATCC AGTGACCCTT ATGTCACCGT CCAGGTTGGG 
AAGACCAAGA AAAGGACAAA AACCATCTAC GGGAACCTCA ACCCAGTGTG GGAAGAGAAT 
TTTCATTTTG AATGTCACAA CTCCTCTGAC CGGATCAAGG TTCGTGTTTG GGATGAAGAT 
GACGACATAA AATCCCGTGT GAAACAAAGG TTTAAGAGGG AGTCTGATGA CTTCCTAGGG 
CAGACAATCA TCGAGGTGCG GACGCTTAGC GGCGAGATGG ATGTGTGGTA CAATCTGGAC 
AAGAGAACGG ACAAGTCGGC GGTGTCGGGG GCCATCCGGC TTCACATCAG TGTGGAGATC 
AAAGGGGAGG AGAAGGTGGC GCCCTACCAT GTCCAGTACA CCTGTCTGCA TGAGAACCTG 
TTCCACTTTG TGACGGACGT GCAGAACAAT GGTGTGGTGA AGATTCCCGA TGCCAAGGGT 
GACGACGCCT GGAAGGTTTA CTACGATGAG ACGGCCCAGG AGATCGTGGA TGAGTTTGCC 
ATGCGCTATG GTGTTGAGTC CATCTACCAA GCCATGACCC ACTTTGCCTG CCTCTCCTCC 
AAGTATATGT GCCCTGGGGT ACCCGCTGTC ATGAGCACCC TGCTTGCCAA CATCAACGCC 
TACTACGCAC ACACCACCGC CTCCACCAAC GTGTCTGCCT CTGACCGCTT CGCTGCCTCT 
AATTTCGGGA AAGAGCGCTT TGTGAAACTT CTGGACCAGC TACACAATTC TCTGCGGATC 
GACCTGTCCA TGTACCGGAA CAACTTCCCA GCCAGCAGCC CTGAGCGGCT GCAGGATCTC 
AAGTCCACGG TGGACCTGCT CACCAGCATC ACCTTCTTCC GGATGAAGGT TCAGGAACTG 
CAGAGCCCGC CGCGTGCCAG CCAGGTAGTG AAAGACTGTG TGAAGGCCTG CCTCAACTCC 
ACCTATGAGT ACATCTTCAA CAACTGCCAT GAGCTCTATG GCCGGGAGTA CCAAACGGAT 
CCGGCCAAGA AGGGGGAGGT TCCCCCAGAG GAGCAAGGCC CTAGCATCAA GAACCTGGAT 
TTCTGGTCCA AGCTGATCAC CCTCATCGTG TCTATCATCG AGGAGGATAA GAATTCTTAC 
ACACCCTGCC TCAATCAGTT TCCCCAGGAG CTCAATGTGG GGAAGATCAG TGCTGAGGTG 
ATGTGGAGCC TATTTGCCCA AGACATGAAG TACGCCATGG AGGAACATGA CAAACACCGG 
CTGTGTAAGA GCGCAGATTA CATGAACCTG CACTTCAAGG TGAAGTGGCT GTACAATGAG 
TACGTGGCCG AGCTGCCCAC CTTCAAGGAC CGTGTGCCTG AGTACCCTGC GTGGTTTGAG 
CCCTTCGTCA TCCAGTGGTT GGATGAGAAT GAGGAGGTGT CCCGGGACTT CCTGCATGGT 
GCCCTCGAGC GGGACAAGAA GGACGGGTTC CAGCAGACCT CAGAGCACGC CCTGTTCTCT 
TGCTCAGTGG TGGACGTCTT CTCCCAGCTT AACCAGAGTT TCGAGATCAT CAAGAAGCTG 
GAGTGTCCTG ACCCCCAGAT CGTGGGCCAT TACATGCGGC GTTTTGCCAA GACCATTAGC 
AATGTGCTTC TCCAGTATGC AGACATCGTT TCCAAGGACT TCGCCTCCTA CTGCTCCAAG 
GAGAAGGAGA AAGTGCCCTG CATCCTCATG AACAACACAC AGCAACTACG AGTGCAGCTG 
GAGAAGATGT TTGAGGCGAT GGGCGGGAAG GAGCTGGATG CCGAGGCCAG TGGCACCCTG 
AAGGAGCTGC AGGTGAAACT CAACAATGTC CTGGATGAAC TCAGCCATGT GTTTGCCACC 
AGCTTCCAGC CACACATTGA GGAATGCGTC AGACAGATGG GTGACATCCT GAGCCAGGTG 
AAGGGCACAG GCAATGTACC TGCCAGTGCC TGCAGCAGCG TGGCCCAGGA TGCAGACAAT 
GTGCTGCAGC CCATCATGGA TCTTCTGGAC AGCAACCTCA CCCTGTTTGC CAAAATCTGT 
GAGAAGACAG TTCTGAAGCG GGTGCTGAAG GAGCTCTGGA AGCTGGTGAT GAACACAATG 
GAGAAGACCA TCGTCCTGCC ACCGCTCACT GACCAGACGA TGATTGGCAC CCTCTTGAGA 
AAACATGGCA AGGGCCTAGA AAAGGGCAGG GTGAAACTGC CAAGCCACTC AGACGGGACA 
CAAATGATCT TCAATGCTGC CAAGGAGCTG GGCCAGCTGT CCAAACTTAA GGATCACATG 
GTGCGGGAAG AAGCCAAGAG CTTGACCCCG AAGCAGTGTG CTGTTGTTGA ACTGGCCCTG 
GACACCATCA AGCAATACTT CCACGCGGGG GGCGTTGGCC TCAAGAAGAC CTTCCTGGAG 
AAGAGCCCGG ACCTCCAGTC CCTGCGCTAC GCCCTGTCGC TCTACACGCA AGCCACCGAC 
CTGCTCATCA AAACCTTCGT GCAGACGCAG TCAGCGCAGG GCTCTGGTGT GGAGGACCCT 
GTAGGTGAAG TGTCCGTCCA CGTGGAGCTG TTCACACATC CAGGAACTGG GGAACAGAAG 
GTCACAGTGA AGGTGGTGGC CGCCAATGAC CTCAAGTGGC AGACTTCTGG CATCTTCCGC 
CCGTTCATCG AGGTCAACAT CGTTGGACCT CAGCTTAGCG ACAAGAAACG CAAGTTCGCC 
ACCAAATCCA AAAACAACAG CTGGGCGCCC AAGTATAACG AGAGCTTCCA GTTCTCCCTG 
AGCGCCGACG CGGGCCCCGA GTGCTACGAG CTGCAGGTGT GCGTGAAGGA CTACTGCTTT 
GCGCGCGAAG ACCGCACGGT GGGGCTGGCG GTGCTGCAAC TGCGAGAGCT GGCCCAGCGC 
GGGAGCGCCG CGTGCTGGCT TCCACTCGGC CGCCGCATCC ACATGGACGA CACGGGGCTC 
ACAGTGCTGC GCATCCTGTC GCAGCGCAGC AACGATGAGG TGGCCAAGGA ATTCGTCAAG 
CTCAAGTCGG ACACGCGCTC AGCCGAGGAG GGCGGTGCCG CGCCTGCGCC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Unc13a ( XM_017312878.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168367.1 Mus musculus unc-13 homolog A (C. elegans) (Unc13a), transcript variant X6, mRNA.

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CloneID	OMu82528
Clone ID Related Accession (Same CDS sequence)	XM_017312878.1 , XM_017312878.2
Accession Version	XM_017312878.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5142bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein unc-13 homolog A isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTGC TGTGCGTGGG AGTCAAAAAA GCCAAGTTTG ACGGTGCCCA AGAGAAGTTC 
AACACATACG TGACGCTGAA GGTGCAGAAC GTGAAGAGCA CTACCATAGC TGTACGTGGC 
AGCCAGCCCA GCTGGGAGCA GGACTTCATG TTTGAGATCA ACCGCCTGGA TCTAGGCCTG 
ACCGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGTAC TGTGTGGATC 
CCGCTGCGGA CCATCCGCCA GTCCAATGAG GAGGGCCCAG GAGAGTGGCT CACACTGGAC 
TCTCAGGCCA TCATGGCGGA CAGTGAGATC TGTGGGACCA AGGACCCCAC CTTCCATCGC 
ATCCTCCTGG ACGCTCATTT CGAGCTGCCG TTGGACATCC CTGAGGAGGA GGCACGCTAC 
TGGGCCAAGA AGCTGGAGCA GCTGAATGCC ATGCGTGACC AGGATGAGGT GAGTCACCTC 
CAGTACTCCT TCCAGGACCA GCAGGACAAG CCGCTGCCAG TGCCCAGCAG CCAGTGCTGC 
AACTGGAATT ACTTTGGCTG GGGAGAACAG AATGACGACC CCGACAGTGC GGTGGATGAC 
CGGGACAGTG ACTATAGGAG TGAGACCAGC AACAGCATCC CACCGCCCTA CTATACGACT 
TCGCAGCCCA ACGCCTCTGT GCACCAGTAC TCCGTGCGAC CGCCCCCTCT GGGGTCCCGG 
GAGTCCTACA GTGACTCCAT GCACAGCTAT GAGGAGTTCT CTGAGCCGCG GGCGCTCAGC 
CCCACAGGCA GCAGCCGCTA TGCTTCTTCC GGGGAGCTGA GCCAGGGAAG CTCACAGCTG 
AGCGAGGACT TCGACCCTGA TGAGCACAGC CTGCAGGGCT CGGAGCTGGA TGACGAGAGG 
GACCGCGATT CTTATCACTC CTGTCACAGC TCCGTGAGTT ACCACAAGGA CTCACCCCGC 
TGGGACCAGG ACGATGAGGA CCTGGAGGAC CTGGAGGACC TGGAAGATGA GGAGCTGCCC 
GAGGAGGAGG AGGAGTTGGA GGAGGAGGGG GAGGAGGAAT TGGAGGAAGA GGATTTGGAG 
GAGGAGGAGG AGGTGCCTGA TGACCTGGCC AGCTACACCC AGCAGGAGGA CACCACTGTG 
GCTGAGCCCA AAGAGTTCAA GCGGATCAGC TTCCCAACGG CTGCGCCTCA GAAGGATGAC 
AAGGTTTCAG CTGTGCCTAC AGAGGCCCCC GAGGTGGCCA AAGGCATCCC CAAAGCAGCC 
ACACCCGAGG AGAAGGCAGC TGCAGAGCGT GCGCAGGAAG CCGAGCCCCC CAAGTCTGAG 
GAGAGTTTCA GATCTCGAGA GGAGGAAGAG GGCCAGGAAG GGCAGGATGC CATGTCCAGA 
GCCAAAGCCA ACTGGTTGCG AGCCTTCAAC AAGGTGCGCA TGCAGCTGCA GGAGGCCCGA 
GGAGAAGGGG ATATGTCTAA GTCTCTATGG TTCAAAGGCG GTCCTGGTGG TGGCCTTATC 
ATCATTGACA GCATGCCAGA CATCAGGAAA CGGAAGCCCA TTCCCCTCGT GAGCGACCTG 
GCTATGTCAT TGGTGCAGTC TCGGAAGGCA GGCATCACCT CGGCCTTGGC CTCCAGCACG 
TTGAACAATG AAGAGCTGAA AAATCACGTT TACAAGAAGA CCCTGCAAGC CTTAATCTAC 
CCCATCTCCT GCACCACGCC GCACAATTTC GAGGTGTGGA CGGCCACCAC GCCCACCTAC 
TGCTATGAGT GCGAGGGGCT GCTGTGGGGC ATCGCGCGGC AGGGCATGCG ATGCACCGAG 
TGCGGCGTTA AGTGCCACGA GAAGTGCCAG GACCTGCTCA ATGCGGATTG CCTGCAGCGG 
GCGGCTGAGA AGAGTTCCAA GCATGGCGCT GAAGACCGCA CGCAGAACAT CATCATGGTG 
CTGAAGGACC GCATGAAGAT CCGCGAGCGC AACAAGCCTG AGATCTTCGA GCTTATCCAA 
GAGATCTTCG CGGTCACCAA GAGCGCACAC ACACAGCAGA TGAAAGCTGT CAAGCAGAGT 
GTGCTGGACG GCACATCCAA GTGGTCTGCC AAAATTAGCA TCACAGTGGT CTGTGCCCAG 
GGCTTGCAGG CCAAGGACAA GACAGGATCC AGTGACCCTT ATGTCACCGT CCAGGTTGGG 
AAGACCAAGA AAAGGACAAA AACCATCTAC GGGAACCTCA ACCCAGTGTG GGAAGAGAAT 
TTTCATTTTG AATGTCACAA CTCCTCTGAC CGGATCAAGG TTCGTGTTTG GGATGAAGAT 
GACGACATAA AATCCCGTGT GAAACAAAGG TTTAAGAGGG AGTCTGATGA CTTCCTAGGG 
CAGACAATCA TCGAGGTGCG GACGCTTAGC GGCGAGATGG ATGTGTGGTA CAATCTGGAC 
AAGAGAACGG ACAAGTCGGC GGTGTCGGGG GCCATCCGGC TTCACATCAG TGTGGAGATC 
AAAGGGGAGG AGAAGGTGGC GCCCTACCAT GTCCAGTACA CCTGTCTGCA TGAGAACCTG 
TTCCACTTTG TGACGGACGT GCAGAACAAT GGTGTGGTGA AGATTCCCGA TGCCAAGGGT 
GACGACGCCT GGAAGGTTTA CTACGATGAG ACGGCCCAGG AGATCGTGGA TGAGTTTGCC 
ATGCGCTATG GTGTTGAGTC CATCTACCAA GCCATGACCC ACTTTGCCTG CCTCTCCTCC 
AAGTATATGT GCCCTGGGGT ACCCGCTGTC ATGAGCACCC TGCTTGCCAA CATCAACGCC 
TACTACGCAC ACACCACCGC CTCCACCAAC GTGTCTGCCT CTGACCGCTT CGCTGCCTCT 
AATTTCGGGA AAGAGCGCTT TGTGAAACTT CTGGACCAGC TACACAATTC TCTGCGGATC 
GACCTGTCCA TGTACCGGAA CAACTTCCCA GCCAGCAGCC CTGAGCGGCT GCAGGATCTC 
AAGTCCACGG TGGACCTGCT CACCAGCATC ACCTTCTTCC GGATGAAGGT TCAGGAACTG 
CAGAGCCCGC CGCGTGCCAG CCAGGTAGTG AAAGACTGTG TGAAGGCCTG CCTCAACTCC 
ACCTATGAGT ACATCTTCAA CAACTGCCAT GAGCTCTATG GCCGGGAGTA CCAAACGGAT 
CCGGCCAAGA AGGGGGAGGT TCCCCCAGAG GAGCAAGGCC CTAGCATCAA GAACCTGGAT 
TTCTGGTCCA AGCTGATCAC CCTCATCGTG TCTATCATCG AGGAGGATAA GAATTCTTAC 
ACACCCTGCC TCAATCAGTT TCCCCAGGAG CTCAATGTGG GGAAGATCAG TGCTGAGGTG 
ATGTGGAGCC TATTTGCCCA AGACATGAAG TACGCCATGG AGGAACATGA CAAACACCGG 
CTGTGTAAGA GCGCAGATTA CATGAACCTG CACTTCAAGG TGAAGTGGCT GTACAATGAG 
TACGTGGCCG AGCTGCCCAC CTTCAAGGAC CGTGTGCCTG AGTACCCTGC GTGGTTTGAG 
CCCTTCGTCA TCCAGTGGTT GGATGAGAAT GAGGAGGTGT CCCGGGACTT CCTGCATGGT 
GCCCTCGAGC GGGACAAGAA GGACGGGTTC CAGCAGACCT CAGAGCACGC CCTGTTCTCT 
TGCTCAGTGG TGGACGTCTT CTCCCAGCTT AACCAGAGTT TCGAGATCAT CAAGAAGCTG 
GAGTGTCCTG ACCCCCAGAT CGTGGGCCAT TACATGCGGC GTTTTGCCAA GACCATTAGC 
AATGTGCTTC TCCAGTATGC AGACATCGTT TCCAAGGACT TCGCCTCCTA CTGCTCCAAG 
GAGAAGGAGA AAGTGCCCTG CATCCTCATG AACAACACAC AGCAACTACG AGTGCAGCTG 
GAGAAGATGT TTGAGGCGAT GGGCGGGAAG GAGCTGGATG CCGAGGCCAG TGGCACCCTG 
AAGGAGCTGC AGGTGAAACT CAACAATGTC CTGGATGAAC TCAGCCATGT GTTTGCCACC 
AGCTTCCAGC CACACATTGA GGAATGCGTC AGACAGATGG GTGACATCCT GAGCCAGGTG 
AAGGGCACAG GCAATGTACC TGCCAGTGCC TGCAGCAGCG TGGCCCAGGA TGCAGACAAT 
GTGCTGCAGC CCATCATGGA TCTTCTGGAC AGCAACCTCA CCCTGTTTGC CAAAATCTGT 
GAGAAGACAG TTCTGAAGCG GGTGCTGAAG GAGCTCTGGA AGCTGGTGAT GAACACAATG 
GAGAAGACCA TCGTCCTGCC ACCGCTCACT GACCAGACGA TGATTGGGAC ACAAATGATC 
TTCAATGCTG CCAAGGAGCT GGGCCAGCTG TCCAAACTTA AGGATCACAT GGTGCGGGAA 
GAAGCCAAGA GCTTGACCCC GAAGCAGTGT GCTGTTGTTG AACTGGCCCT GGACACCATC 
AAGCAATACT TCCACGCGGG GGGCGTTGGC CTCAAGAAGA CCTTCCTGGA GAAGAGCCCG 
GACCTCCAGT CCCTGCGCTA CGCCCTGTCG CTCTACACGC AAGCCACCGA CCTGCTCATC 
AAAACCTTCG TGCAGACGCA GTCAGCGCAG GTCCATGGTG GAAAAGGGAC TAGGTTTACC 
CTTAGTGAAG ACGTTTGTCC TGAGATGGGC TCTGGTGTGG AGGACCCTGT AGGTGAAGTG 
TCCGTCCACG TGGAGCTGTT CACACATCCA GGAACTGGGG AACAGAAGGT CACAGTGAAG 
GTGGTGGCCG CCAATGACCT CAAGTGGCAG ACTTCTGGCA TCTTCCGCCC GTTCATCGAG 
GTCAACATCG TTGGACCTCA GCTTAGCGAC AAGAAACGCA AGTTCGCCAC CAAATCCAAA 
AACAACAGCT GGGCGCCCAA GTATAACGAG AGCTTCCAGT TCTCCCTGAG CGCCGACGCG 
GGCCCCGAGT GCTACGAGCT GCAGGTGTGC GTGAAGGACT ACTGCTTTGC GCGCGAAGAC 
CGCACGGTGG GGCTGGCGGT GCTGCAACTG CGAGAGCTGG CCCAGCGCGG GAGCGCCGCG 
TGCTGGCTTC CACTCGGCCG CCGCATCCAC ATGGACGACA CGGGGCTCAC AGTGCTGCGC 
ATCCTGTCGC AGCGCAGCAA CGATGAGGTG GCCAAGGAAT TCGTCAAGCT CAAGTCGGAC 
ACGCGCTCAG CCGAGGAGGG CGGTGCCGCG CCTGCGCCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168367.1
CDS	125..5266
Translation	MSLLCVGVKKAKFDGAQEKFNTYVTLKVQNVKSTTIAVRGSQPSWEQDFMFEINRLDLGLTVEVWNKGLIWDTMVGTVWIPLRTIRQSNEEGPGEWLTLDSQAIMADSEICGTKDPTFHRILLDAHFELPLDIPEEEARYWAKKLEQLNAMRDQDEVSHLQYSFQDQQDKPLPVPSSQCCNWNYFGWGEQNDDPDSAVDDRDSDYRSETSNSIPPPYYTTSQPNASVHQYSVRPPPLGSRESYSDSMHSYEEFSEPRALSPTGSSRYASSGELSQGSSQLSEDFDPDEHSLQGSELDDERDRDSYHSCHSSVSYHKDSPRWDQDDEDLEDLEDLEDEELPEEEEELEEEGEEELEEEDLEEEEEVPDDLASYTQQEDTTVAEPKEFKRISFPTAAPQKDDKVSAVPTEAPEVAKGIPKAATPEEKAAAERAQEAEPPKSEESFRSREEEEGQEGQDAMSRAKANWLRAFNKVRMQLQEARGEGDMSKSLWFKGGPGGGLIIIDSMPDIRKRKPIPLVSDLAMSLVQSRKAGITSALASSTLNNEELKNHVYKKTLQALIYPISCTTPHNFEVWTATTPTYCYECEGLLWGIARQGMRCTECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMVLKDRMKIRERNKPEIFELIQEIFAVTKSAHTQQMKAVKQSVLDGTSKWSAKISITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIYGNLNPVWEENFHFECHNSSDRIKVRVWDEDDDIKSRVKQRFKRESDDFLGQTIIEVRTLSGEMDVWYNLDKRTDKSAVSGAIRLHISVEIKGEEKVAPYHVQYTCLHENLFHFVTDVQNNGVVKIPDAKGDDAWKVYYDETAQEIVDEFAMRYGVESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSMYRNNFPASSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHELYGREYQTDPAKKGEVPPEEQGPSIKNLDFWSKLITLIVSIIEEDKNSYTPCLNQFPQELNVGKISAEVMWSLFAQDMKYAMEEHDKHRLCKSADYMNLHFKVKWLYNEYVAELPTFKDRVPEYPAWFEPFVIQWLDENEEVSRDFLHGALERDKKDGFQQTSEHALFSCSVVDVFSQLNQSFEIIKKLECPDPQIVGHYMRRFAKTISNVLLQYADIVSKDFASYCSKEKEKVPCILMNNTQQLRVQLEKMFEAMGGKELDAEASGTLKELQVKLNNVLDELSHVFATSFQPHIEECVRQMGDILSQVKGTGNVPASACSSVAQDADNVLQPIMDLLDSNLTLFAKICEKTVLKRVLKELWKLVMNTMEKTIVLPPLTDQTMIGTQMIFNAAKELGQLSKLKDHMVREEAKSLTPKQCAVVELALDTIKQYFHAGGVGLKKTFLEKSPDLQSLRYALSLYTQATDLLIKTFVQTQSAQVHGGKGTRFTLSEDVCPEMGSGVEDPVGEVSVHVELFTHPGTGEQKVTVKVVAANDLKWQTSGIFRPFIEVNIVGPQLSDKKRKFATKSKNNSWAPKYNESFQFSLSADAGPECYELQVCVKDYCFAREDRTVGLAVLQLRELAQRGSAACWLPLGRRIHMDDTGLTVLRILSQRSNDEVAKEFVKLKSDTRSAEEGGAAPAP

Target ORF information:

RefSeq Version	XM_017312878.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus unc-13 homolog A (C. elegans) (Unc13a), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312878.1

ORF Insert Sequence:

ATGTCTCTGC TGTGCGTGGG AGTCAAAAAA GCCAAGTTTG ACGGTGCCCA AGAGAAGTTC 
AACACATACG TGACGCTGAA GGTGCAGAAC GTGAAGAGCA CTACCATAGC TGTACGTGGC 
AGCCAGCCCA GCTGGGAGCA GGACTTCATG TTTGAGATCA ACCGCCTGGA TCTAGGCCTG 
ACCGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGTAC TGTGTGGATC 
CCGCTGCGGA CCATCCGCCA GTCCAATGAG GAGGGCCCAG GAGAGTGGCT CACACTGGAC 
TCTCAGGCCA TCATGGCGGA CAGTGAGATC TGTGGGACCA AGGACCCCAC CTTCCATCGC 
ATCCTCCTGG ACGCTCATTT CGAGCTGCCG TTGGACATCC CTGAGGAGGA GGCACGCTAC 
TGGGCCAAGA AGCTGGAGCA GCTGAATGCC ATGCGTGACC AGGATGAGGT GAGTCACCTC 
CAGTACTCCT TCCAGGACCA GCAGGACAAG CCGCTGCCAG TGCCCAGCAG CCAGTGCTGC 
AACTGGAATT ACTTTGGCTG GGGAGAACAG AATGACGACC CCGACAGTGC GGTGGATGAC 
CGGGACAGTG ACTATAGGAG TGAGACCAGC AACAGCATCC CACCGCCCTA CTATACGACT 
TCGCAGCCCA ACGCCTCTGT GCACCAGTAC TCCGTGCGAC CGCCCCCTCT GGGGTCCCGG 
GAGTCCTACA GTGACTCCAT GCACAGCTAT GAGGAGTTCT CTGAGCCGCG GGCGCTCAGC 
CCCACAGGCA GCAGCCGCTA TGCTTCTTCC GGGGAGCTGA GCCAGGGAAG CTCACAGCTG 
AGCGAGGACT TCGACCCTGA TGAGCACAGC CTGCAGGGCT CGGAGCTGGA TGACGAGAGG 
GACCGCGATT CTTATCACTC CTGTCACAGC TCCGTGAGTT ACCACAAGGA CTCACCCCGC 
TGGGACCAGG ACGATGAGGA CCTGGAGGAC CTGGAGGACC TGGAAGATGA GGAGCTGCCC 
GAGGAGGAGG AGGAGTTGGA GGAGGAGGGG GAGGAGGAAT TGGAGGAAGA GGATTTGGAG 
GAGGAGGAGG AGGTGCCTGA TGACCTGGCC AGCTACACCC AGCAGGAGGA CACCACTGTG 
GCTGAGCCCA AAGAGTTCAA GCGGATCAGC TTCCCAACGG CTGCGCCTCA GAAGGATGAC 
AAGGTTTCAG CTGTGCCTAC AGAGGCCCCC GAGGTGGCCA AAGGCATCCC CAAAGCAGCC 
ACACCCGAGG AGAAGGCAGC TGCAGAGCGT GCGCAGGAAG CCGAGCCCCC CAAGTCTGAG 
GAGAGTTTCA GATCTCGAGA GGAGGAAGAG GGCCAGGAAG GGCAGGATGC CATGTCCAGA 
GCCAAAGCCA ACTGGTTGCG AGCCTTCAAC AAGGTGCGCA TGCAGCTGCA GGAGGCCCGA 
GGAGAAGGGG ATATGTCTAA GTCTCTATGG TTCAAAGGCG GTCCTGGTGG TGGCCTTATC 
ATCATTGACA GCATGCCAGA CATCAGGAAA CGGAAGCCCA TTCCCCTCGT GAGCGACCTG 
GCTATGTCAT TGGTGCAGTC TCGGAAGGCA GGCATCACCT CGGCCTTGGC CTCCAGCACG 
TTGAACAATG AAGAGCTGAA AAATCACGTT TACAAGAAGA CCCTGCAAGC CTTAATCTAC 
CCCATCTCCT GCACCACGCC GCACAATTTC GAGGTGTGGA CGGCCACCAC GCCCACCTAC 
TGCTATGAGT GCGAGGGGCT GCTGTGGGGC ATCGCGCGGC AGGGCATGCG ATGCACCGAG 
TGCGGCGTTA AGTGCCACGA GAAGTGCCAG GACCTGCTCA ATGCGGATTG CCTGCAGCGG 
GCGGCTGAGA AGAGTTCCAA GCATGGCGCT GAAGACCGCA CGCAGAACAT CATCATGGTG 
CTGAAGGACC GCATGAAGAT CCGCGAGCGC AACAAGCCTG AGATCTTCGA GCTTATCCAA 
GAGATCTTCG CGGTCACCAA GAGCGCACAC ACACAGCAGA TGAAAGCTGT CAAGCAGAGT 
GTGCTGGACG GCACATCCAA GTGGTCTGCC AAAATTAGCA TCACAGTGGT CTGTGCCCAG 
GGCTTGCAGG CCAAGGACAA GACAGGATCC AGTGACCCTT ATGTCACCGT CCAGGTTGGG 
AAGACCAAGA AAAGGACAAA AACCATCTAC GGGAACCTCA ACCCAGTGTG GGAAGAGAAT 
TTTCATTTTG AATGTCACAA CTCCTCTGAC CGGATCAAGG TTCGTGTTTG GGATGAAGAT 
GACGACATAA AATCCCGTGT GAAACAAAGG TTTAAGAGGG AGTCTGATGA CTTCCTAGGG 
CAGACAATCA TCGAGGTGCG GACGCTTAGC GGCGAGATGG ATGTGTGGTA CAATCTGGAC 
AAGAGAACGG ACAAGTCGGC GGTGTCGGGG GCCATCCGGC TTCACATCAG TGTGGAGATC 
AAAGGGGAGG AGAAGGTGGC GCCCTACCAT GTCCAGTACA CCTGTCTGCA TGAGAACCTG 
TTCCACTTTG TGACGGACGT GCAGAACAAT GGTGTGGTGA AGATTCCCGA TGCCAAGGGT 
GACGACGCCT GGAAGGTTTA CTACGATGAG ACGGCCCAGG AGATCGTGGA TGAGTTTGCC 
ATGCGCTATG GTGTTGAGTC CATCTACCAA GCCATGACCC ACTTTGCCTG CCTCTCCTCC 
AAGTATATGT GCCCTGGGGT ACCCGCTGTC ATGAGCACCC TGCTTGCCAA CATCAACGCC 
TACTACGCAC ACACCACCGC CTCCACCAAC GTGTCTGCCT CTGACCGCTT CGCTGCCTCT 
AATTTCGGGA AAGAGCGCTT TGTGAAACTT CTGGACCAGC TACACAATTC TCTGCGGATC 
GACCTGTCCA TGTACCGGAA CAACTTCCCA GCCAGCAGCC CTGAGCGGCT GCAGGATCTC 
AAGTCCACGG TGGACCTGCT CACCAGCATC ACCTTCTTCC GGATGAAGGT TCAGGAACTG 
CAGAGCCCGC CGCGTGCCAG CCAGGTAGTG AAAGACTGTG TGAAGGCCTG CCTCAACTCC 
ACCTATGAGT ACATCTTCAA CAACTGCCAT GAGCTCTATG GCCGGGAGTA CCAAACGGAT 
CCGGCCAAGA AGGGGGAGGT TCCCCCAGAG GAGCAAGGCC CTAGCATCAA GAACCTGGAT 
TTCTGGTCCA AGCTGATCAC CCTCATCGTG TCTATCATCG AGGAGGATAA GAATTCTTAC 
ACACCCTGCC TCAATCAGTT TCCCCAGGAG CTCAATGTGG GGAAGATCAG TGCTGAGGTG 
ATGTGGAGCC TATTTGCCCA AGACATGAAG TACGCCATGG AGGAACATGA CAAACACCGG 
CTGTGTAAGA GCGCAGATTA CATGAACCTG CACTTCAAGG TGAAGTGGCT GTACAATGAG 
TACGTGGCCG AGCTGCCCAC CTTCAAGGAC CGTGTGCCTG AGTACCCTGC GTGGTTTGAG 
CCCTTCGTCA TCCAGTGGTT GGATGAGAAT GAGGAGGTGT CCCGGGACTT CCTGCATGGT 
GCCCTCGAGC GGGACAAGAA GGACGGGTTC CAGCAGACCT CAGAGCACGC CCTGTTCTCT 
TGCTCAGTGG TGGACGTCTT CTCCCAGCTT AACCAGAGTT TCGAGATCAT CAAGAAGCTG 
GAGTGTCCTG ACCCCCAGAT CGTGGGCCAT TACATGCGGC GTTTTGCCAA GACCATTAGC 
AATGTGCTTC TCCAGTATGC AGACATCGTT TCCAAGGACT TCGCCTCCTA CTGCTCCAAG 
GAGAAGGAGA AAGTGCCCTG CATCCTCATG AACAACACAC AGCAACTACG AGTGCAGCTG 
GAGAAGATGT TTGAGGCGAT GGGCGGGAAG GAGCTGGATG CCGAGGCCAG TGGCACCCTG 
AAGGAGCTGC AGGTGAAACT CAACAATGTC CTGGATGAAC TCAGCCATGT GTTTGCCACC 
AGCTTCCAGC CACACATTGA GGAATGCGTC AGACAGATGG GTGACATCCT GAGCCAGGTG 
AAGGGCACAG GCAATGTACC TGCCAGTGCC TGCAGCAGCG TGGCCCAGGA TGCAGACAAT 
GTGCTGCAGC CCATCATGGA TCTTCTGGAC AGCAACCTCA CCCTGTTTGC CAAAATCTGT 
GAGAAGACAG TTCTGAAGCG GGTGCTGAAG GAGCTCTGGA AGCTGGTGAT GAACACAATG 
GAGAAGACCA TCGTCCTGCC ACCGCTCACT GACCAGACGA TGATTGGGAC ACAAATGATC 
TTCAATGCTG CCAAGGAGCT GGGCCAGCTG TCCAAACTTA AGGATCACAT GGTGCGGGAA 
GAAGCCAAGA GCTTGACCCC GAAGCAGTGT GCTGTTGTTG AACTGGCCCT GGACACCATC 
AAGCAATACT TCCACGCGGG GGGCGTTGGC CTCAAGAAGA CCTTCCTGGA GAAGAGCCCG 
GACCTCCAGT CCCTGCGCTA CGCCCTGTCG CTCTACACGC AAGCCACCGA CCTGCTCATC 
AAAACCTTCG TGCAGACGCA GTCAGCGCAG GTCCATGGTG GAAAAGGGAC TAGGTTTACC 
CTTAGTGAAG ACGTTTGTCC TGAGATGGGC TCTGGTGTGG AGGACCCTGT AGGTGAAGTG 
TCCGTCCACG TGGAGCTGTT CACACATCCA GGAACTGGGG AACAGAAGGT CACAGTGAAG 
GTGGTGGCCG CCAATGACCT CAAGTGGCAG ACTTCTGGCA TCTTCCGCCC GTTCATCGAG 
GTCAACATCG TTGGACCTCA GCTTAGCGAC AAGAAACGCA AGTTCGCCAC CAAATCCAAA 
AACAACAGCT GGGCGCCCAA GTATAACGAG AGCTTCCAGT TCTCCCTGAG CGCCGACGCG 
GGCCCCGAGT GCTACGAGCT GCAGGTGTGC GTGAAGGACT ACTGCTTTGC GCGCGAAGAC 
CGCACGGTGG GGCTGGCGGT GCTGCAACTG CGAGAGCTGG CCCAGCGCGG GAGCGCCGCG 
TGCTGGCTTC CACTCGGCCG CCGCATCCAC ATGGACGACA CGGGGCTCAC AGTGCTGCGC 
ATCCTGTCGC AGCGCAGCAA CGATGAGGTG GCCAAGGAAT TCGTCAAGCT CAAGTCGGAC 
ACGCGCTCAG CCGAGGAGGG CGGTGCCGCG CCTGCGCCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Unc13a ( XM_017312879.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168368.1 Mus musculus unc-13 homolog A (C. elegans) (Unc13a), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OMu82529
Clone ID Related Accession (Same CDS sequence)	XM_017312879.1 , XM_017312879.2
Accession Version	XM_017312879.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5136bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein unc-13 homolog A isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTGC TGTGCGTGGG AGTCAAAAAA GCCAAGTTTG ACGGTGCCCA AGAGAAGTTC 
AACACATACG TGACGCTGAA GGTGCAGAAC GTGAAGAGCA CTACCATAGC TGTACGTGGC 
AGCCAGCCCA GCTGGGAGCA GGACTTCATG TTTGAGATCA ACCGCCTGGA TCTAGGCCTG 
ACCGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGTAC TGTGTGGATC 
CCGCTGCGGA CCATCCGCCA GTCCAATGAG GAGGGCCCAG GAGAGTGGCT CACACTGGAC 
TCTCAGGCCA TCATGGCGGA CAGTGAGATC TGTGGGACCA AGGACCCCAC CTTCCATCGC 
ATCCTCCTGG ACGCTCATTT CGAGCTGCCG TTGGACATCC CTGAGGAGGA GGCACGCTAC 
TGGGCCAAGA AGCTGGAGCA GCTGAATGCC ATGCGTGACC AGGATGAGGT GAGTCACCTC 
CAGTACTCCT TCCAGGACCA GCAGGACAAG CCGCTGCCAG TGCCCAGCAG CCAGTGCTGC 
AACTGGAATT ACTTTGGCTG GGGAGAACAG AATGACGACC CCGACAGTGC GGTGGATGAC 
CGGGACAGTG ACTATAGGAG TGAGACCAGC AACAGCATCC CACCGCCCTA CTATACGACT 
TCGCAGCCCA ACGCCTCTGT GCACCAGTAC TCCGTGCGAC CGCCCCCTCT GGGGTCCCGG 
GAGTCCTACA GTGACTCCAT GCACAGCTAT GAGGAGTTCT CTGAGCCGCG GGCGCTCAGC 
CCCACAGGCA GCAGCCGCTA TGCTTCTTCC GGGGAGCTGA GCCAGGGAAG CTCACAGCTG 
AGCGAGGACT TCGACCCTGA TGAGCACAGC CTGCAGGGCT CGGAGCTGGA TGACGAGAGG 
GACCGCGATT CTTATCACTC CTGTCACAGC TCCGTGAGTT ACCACAAGGA CTCACCCCGC 
TGGGACCAGG ACGATGAGGA CCTGGAGGAC CTGGAGGACC TGGAAGATGA GGAGCTGCCC 
GAGGAGGAGG AGGAGTTGGA GGAGGAGGGG GAGGAGGAAT TGGAGGAAGA GGATTTGGAG 
GAGGAGGAGG AGGTGCCTGA TGACCTGGCC AGCTACACCC AGCAGGAGGA CACCACTGTG 
GCTGAGCCCA AAGAGTTCAA GCGGATCAGC TTCCCAACGG CTGCGCCTCA GAAGGATGAC 
AAGGTTTCAG CTGTGCCTAC AGAGGCCCCC GAGGTGGCCA AAGGCATCCC CAAAGCAGCC 
ACACCCGAGG AGAAGGCAGC TGCAGAGCGT GCGCAGGAAG CCGAGCCCCC CAAGTCTGAG 
GAGAGTTTCA GATCTCGAGA GGAGGAAGAG GGCCAGGAAG GGCAGGATGC CATGTCCAGA 
GCCAAAGCCA ACTGGTTGCG AGCCTTCAAC AAGGTGCGCA TGCAGCTGCA GGAGGCCCGA 
GGAGAAGGGG ATATGTCTAA GTCTCTATGG TTCAAAGGCG GTCCTGGTGG TGGCCTTATC 
ATCATTGACA GCATGCCAGA CATCAGGAAA CGGAAGCCCA TTCCCCTCGT GAGCGACCTG 
GCTATGTCAT TGGTGCAGTC TCGGAAGGCA GGCATCACCT CGGCCTTGGC CTCCAGCACG 
TTGAACAATG AAGAGCTGAA AAATCACGTT TACAAGAAGA CCCTGCAAGC CTTAATCTAC 
CCCATCTCCT GCACCACGCC GCACAATTTC GAGGTGTGGA CGGCCACCAC GCCCACCTAC 
TGCTATGAGT GCGAGGGGCT GCTGTGGGGC ATCGCGCGGC AGGGCATGCG ATGCACCGAG 
TGCGGCGTTA AGTGCCACGA GAAGTGCCAG GACCTGCTCA ATGCGGATTG CCTGCAGCGG 
GCGGCTGAGA AGAGTTCCAA GCATGGCGCT GAAGACCGCA CGCAGAACAT CATCATGGTG 
CTGAAGGACC GCATGAAGAT CCGCGAGCGC AACAAGCCTG AGATCTTCGA GCTTATCCAA 
GAGATCTTCG CGGTCACCAA GAGCGCACAC ACACAGCAGA TGAAAGCTGT CAAGCAGAGT 
GTGCTGGACG GCACATCCAA GTGGTCTGCC AAAATTAGCA TCACAGTGGT CTGTGCCCAG 
GGCTTGCAGG CCAAGGACAA GACAGGATCC AGTGACCCTT ATGTCACCGT CCAGGTTGGG 
AAGACCAAGA AAAGGACAAA AACCATCTAC GGGAACCTCA ACCCAGTGTG GGAAGAGAAT 
TTTCATTTTG AATGTCACAA CTCCTCTGAC CGGATCAAGG TTCGTGTTTG GGATGAAGAT 
GACGACATAA AATCCCGTGT GAAACAAAGG TTTAAGAGGG AGTCTGATGA CTTCCTAGGG 
CAGACAATCA TCGAGGTGCG GACGCTTAGC GGCGAGATGG ATGTGTGGTA CAATCTGGAC 
AAGAGAACGG ACAAGTCGGC GGTGTCGGGG GCCATCCGGC TTCACATCAG TGTGGAGATC 
AAAGGGGAGG AGAAGGTGGC GCCCTACCAT GTCCAGTACA CCTGTCTGCA TGAGAACCTG 
TTCCACTTTG TGACGGACGT GCAGAACAAT GGTGTGGTGA AGATTCCCGA TGCCAAGGGT 
GACGACGCCT GGAAGGTTTA CTACGATGAG ACGGCCCAGG AGATCGTGGA TGAGTTTGCC 
ATGCGCTATG GTGTTGAGTC CATCTACCAA GCCATGACCC ACTTTGCCTG CCTCTCCTCC 
AAGTATATGT GCCCTGGGGT ACCCGCTGTC ATGAGCACCC TGCTTGCCAA CATCAACGCC 
TACTACGCAC ACACCACCGC CTCCACCAAC GTGTCTGCCT CTGACCGCTT CGCTGCCTCT 
AATTTCGGGA AAGAGCGCTT TGTGAAACTT CTGGACCAGC TACACAATTC TCTGCGGATC 
GACCTGTCCA TGTACCGGAA CAACTTCCCA GCCAGCAGCC CTGAGCGGCT GCAGGATCTC 
AAGTCCACGG TGGACCTGCT CACCAGCATC ACCTTCTTCC GGATGAAGGT TCAGGAACTG 
CAGAGCCCGC CGCGTGCCAG CCAGGTAGTG AAAGACTGTG TGAAGGCCTG CCTCAACTCC 
ACCTATGAGT ACATCTTCAA CAACTGCCAT GAGCTCTATG GCCGGGAGTA CCAAACGGAT 
CCGGCCAAGA AGGGGGAGGT TCCCCCAGAG GAGCAAGGCC CTAGCATCAA GAACCTGGAT 
TTCTGGTCCA AGCTGATCAC CCTCATCGTG TCTATCATCG AGGAGGATAA GAATTCTTAC 
ACACCCTGCC TCAATCAGTT TCCCCAGGAG CTCAATGTGG GGAAGATCAG TGCTGAGGTG 
ATGTGGAGCC TATTTGCCCA AGACATGAAG TACGCCATGG AGGAACATGA CAAACACCGG 
CTGTGTAAGA GCGCAGATTA CATGAACCTG CACTTCAAGG TGAAGTGGCT GTACAATGAG 
TACGTGGCCG AGCTGCCCAC CTTCAAGGAC CGTGTGCCTG AGTACCCTGC GTGGTTTGAG 
CCCTTCGTCA TCCAGTGGTT GGATGAGAAT GAGGAGGTGT CCCGGGACTT CCTGCATGGT 
GCCCTCGAGC GGGACAAGAA GGACGGGTTC CAGCAGACCT CAGAGCACGC CCTGTTCTCT 
TGCTCAGTGG TGGACGTCTT CTCCCAGCTT AACCAGAGTT TCGAGATCAT CAAGAAGCTG 
GAGTGTCCTG ACCCCCAGAT CGTGGGCCAT TACATGCGGC GTTTTGCCAA GACCATTAGC 
AATGTGCTTC TCCAGTATGC AGACATCGTT TCCAAGGACT TCGCCTCCTA CTGCTCCAAG 
GAGAAGGAGA AAGTGCCCTG CATCCTCATG AACAACACAC AGCAACTACG AGTGCAGCTG 
GAGAAGATGT TTGAGGCGAT GGGCGGGAAG GAGCTGGATG CCGAGGCCAG TGGCACCCTG 
AAGGAGCTGC AGGTGAAACT CAACAATGTC CTGGATGAAC TCAGCCATGT GTTTGCCACC 
AGCTTCCAGC CACACATTGA GGAATGCGTC AGACAGATGG GTGACATCCT GAGCCAGGTG 
AAGGGCACAG GCAATGTACC TGCCAGTGCC TGCAGCAGCG TGGCCCAGGA TGCAGACAAT 
GTGCTGCAGC CCATCATGGA TCTTCTGGAC AGCAACCTCA CCCTGTTTGC CAAAATCTGT 
GAGAAGACAG TTCTGAAGCG GGTGCTGAAG GAGCTCTGGA AGCTGGTGAT GAACACAATG 
GAGAAGACCA TCGTCCTGCC ACCGCTCACT GACCAGACGG GGACACAAAT GATCTTCAAT 
GCTGCCAAGG AGCTGGGCCA GCTGTCCAAA CTTAAGGATC ACATGGTGCG GGAAGAAGCC 
AAGAGCTTGA CCCCGAAGCA GTGTGCTGTT GTTGAACTGG CCCTGGACAC CATCAAGCAA 
TACTTCCACG CGGGGGGCGT TGGCCTCAAG AAGACCTTCC TGGAGAAGAG CCCGGACCTC 
CAGTCCCTGC GCTACGCCCT GTCGCTCTAC ACGCAAGCCA CCGACCTGCT CATCAAAACC 
TTCGTGCAGA CGCAGTCAGC GCAGGTCCAT GGTGGAAAAG GGACTAGGTT TACCCTTAGT 
GAAGACGTTT GTCCTGAGAT GGGCTCTGGT GTGGAGGACC CTGTAGGTGA AGTGTCCGTC 
CACGTGGAGC TGTTCACACA TCCAGGAACT GGGGAACAGA AGGTCACAGT GAAGGTGGTG 
GCCGCCAATG ACCTCAAGTG GCAGACTTCT GGCATCTTCC GCCCGTTCAT CGAGGTCAAC 
ATCGTTGGAC CTCAGCTTAG CGACAAGAAA CGCAAGTTCG CCACCAAATC CAAAAACAAC 
AGCTGGGCGC CCAAGTATAA CGAGAGCTTC CAGTTCTCCC TGAGCGCCGA CGCGGGCCCC 
GAGTGCTACG AGCTGCAGGT GTGCGTGAAG GACTACTGCT TTGCGCGCGA AGACCGCACG 
GTGGGGCTGG CGGTGCTGCA ACTGCGAGAG CTGGCCCAGC GCGGGAGCGC CGCGTGCTGG 
CTTCCACTCG GCCGCCGCAT CCACATGGAC GACACGGGGC TCACAGTGCT GCGCATCCTG 
TCGCAGCGCA GCAACGATGA GGTGGCCAAG GAATTCGTCA AGCTCAAGTC GGACACGCGC 
TCAGCCGAGG AGGGCGGTGC CGCGCCTGCG CCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168368.1
CDS	126..5261
Translation	MSLLCVGVKKAKFDGAQEKFNTYVTLKVQNVKSTTIAVRGSQPSWEQDFMFEINRLDLGLTVEVWNKGLIWDTMVGTVWIPLRTIRQSNEEGPGEWLTLDSQAIMADSEICGTKDPTFHRILLDAHFELPLDIPEEEARYWAKKLEQLNAMRDQDEVSHLQYSFQDQQDKPLPVPSSQCCNWNYFGWGEQNDDPDSAVDDRDSDYRSETSNSIPPPYYTTSQPNASVHQYSVRPPPLGSRESYSDSMHSYEEFSEPRALSPTGSSRYASSGELSQGSSQLSEDFDPDEHSLQGSELDDERDRDSYHSCHSSVSYHKDSPRWDQDDEDLEDLEDLEDEELPEEEEELEEEGEEELEEEDLEEEEEVPDDLASYTQQEDTTVAEPKEFKRISFPTAAPQKDDKVSAVPTEAPEVAKGIPKAATPEEKAAAERAQEAEPPKSEESFRSREEEEGQEGQDAMSRAKANWLRAFNKVRMQLQEARGEGDMSKSLWFKGGPGGGLIIIDSMPDIRKRKPIPLVSDLAMSLVQSRKAGITSALASSTLNNEELKNHVYKKTLQALIYPISCTTPHNFEVWTATTPTYCYECEGLLWGIARQGMRCTECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMVLKDRMKIRERNKPEIFELIQEIFAVTKSAHTQQMKAVKQSVLDGTSKWSAKISITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIYGNLNPVWEENFHFECHNSSDRIKVRVWDEDDDIKSRVKQRFKRESDDFLGQTIIEVRTLSGEMDVWYNLDKRTDKSAVSGAIRLHISVEIKGEEKVAPYHVQYTCLHENLFHFVTDVQNNGVVKIPDAKGDDAWKVYYDETAQEIVDEFAMRYGVESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSMYRNNFPASSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHELYGREYQTDPAKKGEVPPEEQGPSIKNLDFWSKLITLIVSIIEEDKNSYTPCLNQFPQELNVGKISAEVMWSLFAQDMKYAMEEHDKHRLCKSADYMNLHFKVKWLYNEYVAELPTFKDRVPEYPAWFEPFVIQWLDENEEVSRDFLHGALERDKKDGFQQTSEHALFSCSVVDVFSQLNQSFEIIKKLECPDPQIVGHYMRRFAKTISNVLLQYADIVSKDFASYCSKEKEKVPCILMNNTQQLRVQLEKMFEAMGGKELDAEASGTLKELQVKLNNVLDELSHVFATSFQPHIEECVRQMGDILSQVKGTGNVPASACSSVAQDADNVLQPIMDLLDSNLTLFAKICEKTVLKRVLKELWKLVMNTMEKTIVLPPLTDQTGTQMIFNAAKELGQLSKLKDHMVREEAKSLTPKQCAVVELALDTIKQYFHAGGVGLKKTFLEKSPDLQSLRYALSLYTQATDLLIKTFVQTQSAQVHGGKGTRFTLSEDVCPEMGSGVEDPVGEVSVHVELFTHPGTGEQKVTVKVVAANDLKWQTSGIFRPFIEVNIVGPQLSDKKRKFATKSKNNSWAPKYNESFQFSLSADAGPECYELQVCVKDYCFAREDRTVGLAVLQLRELAQRGSAACWLPLGRRIHMDDTGLTVLRILSQRSNDEVAKEFVKLKSDTRSAEEGGAAPAP

Target ORF information:

RefSeq Version	XM_017312879.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus unc-13 homolog A (C. elegans) (Unc13a), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312879.1

ORF Insert Sequence:

ATGTCTCTGC TGTGCGTGGG AGTCAAAAAA GCCAAGTTTG ACGGTGCCCA AGAGAAGTTC 
AACACATACG TGACGCTGAA GGTGCAGAAC GTGAAGAGCA CTACCATAGC TGTACGTGGC 
AGCCAGCCCA GCTGGGAGCA GGACTTCATG TTTGAGATCA ACCGCCTGGA TCTAGGCCTG 
ACCGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGTAC TGTGTGGATC 
CCGCTGCGGA CCATCCGCCA GTCCAATGAG GAGGGCCCAG GAGAGTGGCT CACACTGGAC 
TCTCAGGCCA TCATGGCGGA CAGTGAGATC TGTGGGACCA AGGACCCCAC CTTCCATCGC 
ATCCTCCTGG ACGCTCATTT CGAGCTGCCG TTGGACATCC CTGAGGAGGA GGCACGCTAC 
TGGGCCAAGA AGCTGGAGCA GCTGAATGCC ATGCGTGACC AGGATGAGGT GAGTCACCTC 
CAGTACTCCT TCCAGGACCA GCAGGACAAG CCGCTGCCAG TGCCCAGCAG CCAGTGCTGC 
AACTGGAATT ACTTTGGCTG GGGAGAACAG AATGACGACC CCGACAGTGC GGTGGATGAC 
CGGGACAGTG ACTATAGGAG TGAGACCAGC AACAGCATCC CACCGCCCTA CTATACGACT 
TCGCAGCCCA ACGCCTCTGT GCACCAGTAC TCCGTGCGAC CGCCCCCTCT GGGGTCCCGG 
GAGTCCTACA GTGACTCCAT GCACAGCTAT GAGGAGTTCT CTGAGCCGCG GGCGCTCAGC 
CCCACAGGCA GCAGCCGCTA TGCTTCTTCC GGGGAGCTGA GCCAGGGAAG CTCACAGCTG 
AGCGAGGACT TCGACCCTGA TGAGCACAGC CTGCAGGGCT CGGAGCTGGA TGACGAGAGG 
GACCGCGATT CTTATCACTC CTGTCACAGC TCCGTGAGTT ACCACAAGGA CTCACCCCGC 
TGGGACCAGG ACGATGAGGA CCTGGAGGAC CTGGAGGACC TGGAAGATGA GGAGCTGCCC 
GAGGAGGAGG AGGAGTTGGA GGAGGAGGGG GAGGAGGAAT TGGAGGAAGA GGATTTGGAG 
GAGGAGGAGG AGGTGCCTGA TGACCTGGCC AGCTACACCC AGCAGGAGGA CACCACTGTG 
GCTGAGCCCA AAGAGTTCAA GCGGATCAGC TTCCCAACGG CTGCGCCTCA GAAGGATGAC 
AAGGTTTCAG CTGTGCCTAC AGAGGCCCCC GAGGTGGCCA AAGGCATCCC CAAAGCAGCC 
ACACCCGAGG AGAAGGCAGC TGCAGAGCGT GCGCAGGAAG CCGAGCCCCC CAAGTCTGAG 
GAGAGTTTCA GATCTCGAGA GGAGGAAGAG GGCCAGGAAG GGCAGGATGC CATGTCCAGA 
GCCAAAGCCA ACTGGTTGCG AGCCTTCAAC AAGGTGCGCA TGCAGCTGCA GGAGGCCCGA 
GGAGAAGGGG ATATGTCTAA GTCTCTATGG TTCAAAGGCG GTCCTGGTGG TGGCCTTATC 
ATCATTGACA GCATGCCAGA CATCAGGAAA CGGAAGCCCA TTCCCCTCGT GAGCGACCTG 
GCTATGTCAT TGGTGCAGTC TCGGAAGGCA GGCATCACCT CGGCCTTGGC CTCCAGCACG 
TTGAACAATG AAGAGCTGAA AAATCACGTT TACAAGAAGA CCCTGCAAGC CTTAATCTAC 
CCCATCTCCT GCACCACGCC GCACAATTTC GAGGTGTGGA CGGCCACCAC GCCCACCTAC 
TGCTATGAGT GCGAGGGGCT GCTGTGGGGC ATCGCGCGGC AGGGCATGCG ATGCACCGAG 
TGCGGCGTTA AGTGCCACGA GAAGTGCCAG GACCTGCTCA ATGCGGATTG CCTGCAGCGG 
GCGGCTGAGA AGAGTTCCAA GCATGGCGCT GAAGACCGCA CGCAGAACAT CATCATGGTG 
CTGAAGGACC GCATGAAGAT CCGCGAGCGC AACAAGCCTG AGATCTTCGA GCTTATCCAA 
GAGATCTTCG CGGTCACCAA GAGCGCACAC ACACAGCAGA TGAAAGCTGT CAAGCAGAGT 
GTGCTGGACG GCACATCCAA GTGGTCTGCC AAAATTAGCA TCACAGTGGT CTGTGCCCAG 
GGCTTGCAGG CCAAGGACAA GACAGGATCC AGTGACCCTT ATGTCACCGT CCAGGTTGGG 
AAGACCAAGA AAAGGACAAA AACCATCTAC GGGAACCTCA ACCCAGTGTG GGAAGAGAAT 
TTTCATTTTG AATGTCACAA CTCCTCTGAC CGGATCAAGG TTCGTGTTTG GGATGAAGAT 
GACGACATAA AATCCCGTGT GAAACAAAGG TTTAAGAGGG AGTCTGATGA CTTCCTAGGG 
CAGACAATCA TCGAGGTGCG GACGCTTAGC GGCGAGATGG ATGTGTGGTA CAATCTGGAC 
AAGAGAACGG ACAAGTCGGC GGTGTCGGGG GCCATCCGGC TTCACATCAG TGTGGAGATC 
AAAGGGGAGG AGAAGGTGGC GCCCTACCAT GTCCAGTACA CCTGTCTGCA TGAGAACCTG 
TTCCACTTTG TGACGGACGT GCAGAACAAT GGTGTGGTGA AGATTCCCGA TGCCAAGGGT 
GACGACGCCT GGAAGGTTTA CTACGATGAG ACGGCCCAGG AGATCGTGGA TGAGTTTGCC 
ATGCGCTATG GTGTTGAGTC CATCTACCAA GCCATGACCC ACTTTGCCTG CCTCTCCTCC 
AAGTATATGT GCCCTGGGGT ACCCGCTGTC ATGAGCACCC TGCTTGCCAA CATCAACGCC 
TACTACGCAC ACACCACCGC CTCCACCAAC GTGTCTGCCT CTGACCGCTT CGCTGCCTCT 
AATTTCGGGA AAGAGCGCTT TGTGAAACTT CTGGACCAGC TACACAATTC TCTGCGGATC 
GACCTGTCCA TGTACCGGAA CAACTTCCCA GCCAGCAGCC CTGAGCGGCT GCAGGATCTC 
AAGTCCACGG TGGACCTGCT CACCAGCATC ACCTTCTTCC GGATGAAGGT TCAGGAACTG 
CAGAGCCCGC CGCGTGCCAG CCAGGTAGTG AAAGACTGTG TGAAGGCCTG CCTCAACTCC 
ACCTATGAGT ACATCTTCAA CAACTGCCAT GAGCTCTATG GCCGGGAGTA CCAAACGGAT 
CCGGCCAAGA AGGGGGAGGT TCCCCCAGAG GAGCAAGGCC CTAGCATCAA GAACCTGGAT 
TTCTGGTCCA AGCTGATCAC CCTCATCGTG TCTATCATCG AGGAGGATAA GAATTCTTAC 
ACACCCTGCC TCAATCAGTT TCCCCAGGAG CTCAATGTGG GGAAGATCAG TGCTGAGGTG 
ATGTGGAGCC TATTTGCCCA AGACATGAAG TACGCCATGG AGGAACATGA CAAACACCGG 
CTGTGTAAGA GCGCAGATTA CATGAACCTG CACTTCAAGG TGAAGTGGCT GTACAATGAG 
TACGTGGCCG AGCTGCCCAC CTTCAAGGAC CGTGTGCCTG AGTACCCTGC GTGGTTTGAG 
CCCTTCGTCA TCCAGTGGTT GGATGAGAAT GAGGAGGTGT CCCGGGACTT CCTGCATGGT 
GCCCTCGAGC GGGACAAGAA GGACGGGTTC CAGCAGACCT CAGAGCACGC CCTGTTCTCT 
TGCTCAGTGG TGGACGTCTT CTCCCAGCTT AACCAGAGTT TCGAGATCAT CAAGAAGCTG 
GAGTGTCCTG ACCCCCAGAT CGTGGGCCAT TACATGCGGC GTTTTGCCAA GACCATTAGC 
AATGTGCTTC TCCAGTATGC AGACATCGTT TCCAAGGACT TCGCCTCCTA CTGCTCCAAG 
GAGAAGGAGA AAGTGCCCTG CATCCTCATG AACAACACAC AGCAACTACG AGTGCAGCTG 
GAGAAGATGT TTGAGGCGAT GGGCGGGAAG GAGCTGGATG CCGAGGCCAG TGGCACCCTG 
AAGGAGCTGC AGGTGAAACT CAACAATGTC CTGGATGAAC TCAGCCATGT GTTTGCCACC 
AGCTTCCAGC CACACATTGA GGAATGCGTC AGACAGATGG GTGACATCCT GAGCCAGGTG 
AAGGGCACAG GCAATGTACC TGCCAGTGCC TGCAGCAGCG TGGCCCAGGA TGCAGACAAT 
GTGCTGCAGC CCATCATGGA TCTTCTGGAC AGCAACCTCA CCCTGTTTGC CAAAATCTGT 
GAGAAGACAG TTCTGAAGCG GGTGCTGAAG GAGCTCTGGA AGCTGGTGAT GAACACAATG 
GAGAAGACCA TCGTCCTGCC ACCGCTCACT GACCAGACGG GGACACAAAT GATCTTCAAT 
GCTGCCAAGG AGCTGGGCCA GCTGTCCAAA CTTAAGGATC ACATGGTGCG GGAAGAAGCC 
AAGAGCTTGA CCCCGAAGCA GTGTGCTGTT GTTGAACTGG CCCTGGACAC CATCAAGCAA 
TACTTCCACG CGGGGGGCGT TGGCCTCAAG AAGACCTTCC TGGAGAAGAG CCCGGACCTC 
CAGTCCCTGC GCTACGCCCT GTCGCTCTAC ACGCAAGCCA CCGACCTGCT CATCAAAACC 
TTCGTGCAGA CGCAGTCAGC GCAGGTCCAT GGTGGAAAAG GGACTAGGTT TACCCTTAGT 
GAAGACGTTT GTCCTGAGAT GGGCTCTGGT GTGGAGGACC CTGTAGGTGA AGTGTCCGTC 
CACGTGGAGC TGTTCACACA TCCAGGAACT GGGGAACAGA AGGTCACAGT GAAGGTGGTG 
GCCGCCAATG ACCTCAAGTG GCAGACTTCT GGCATCTTCC GCCCGTTCAT CGAGGTCAAC 
ATCGTTGGAC CTCAGCTTAG CGACAAGAAA CGCAAGTTCG CCACCAAATC CAAAAACAAC 
AGCTGGGCGC CCAAGTATAA CGAGAGCTTC CAGTTCTCCC TGAGCGCCGA CGCGGGCCCC 
GAGTGCTACG AGCTGCAGGT GTGCGTGAAG GACTACTGCT TTGCGCGCGA AGACCGCACG 
GTGGGGCTGG CGGTGCTGCA ACTGCGAGAG CTGGCCCAGC GCGGGAGCGC CGCGTGCTGG 
CTTCCACTCG GCCGCCGCAT CCACATGGAC GACACGGGGC TCACAGTGCT GCGCATCCTG 
TCGCAGCGCA GCAACGATGA GGTGGCCAAG GAATTCGTCA AGCTCAAGTC GGACACGCGC 
TCAGCCGAGG AGGGCGGTGC CGCGCCTGCG CCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Unc13a ( XM_017312880.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168369.1 Mus musculus unc-13 homolog A (C. elegans) (Unc13a), transcript variant X8, mRNA.

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CloneID	OMu82530
Clone ID Related Accession (Same CDS sequence)	XM_017312880.2 , XM_017312880.1
Accession Version	XM_017312880.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5085bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein unc-13 homolog A isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTGC TGTGCGTGGG AGTCAAAAAA GCCAAGTTTG ACGGTGCCCA AGAGAAGTTC 
AACACATACG TGACGCTGAA GGTGCAGAAC GTGAAGAGCA CTACCATAGC TGTACGTGGC 
AGCCAGCCCA GCTGGGAGCA GGACTTCATG TTTGAGATCA ACCGCCTGGA TCTAGGCCTG 
ACCGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGTAC TGTGTGGATC 
CCGCTGCGGA CCATCCGCCA GTCCAATGAG GAGGGCCCAG GAGAGTGGCT CACACTGGAC 
TCTCAGGCCA TCATGGCGGA CAGTGAGATC TGTGGGACCA AGGACCCCAC CTTCCATCGC 
ATCCTCCTGG ACGCTCATTT CGAGCTGCCG TTGGACATCC CTGAGGAGGA GGCACGCTAC 
TGGGCCAAGA AGCTGGAGCA GCTGAATGCC ATGCGTGACC AGGATGAGGT GAGTCACCTC 
CAGTACTCCT TCCAGGACCA GCAGGACAAG CCGCTGCCAG TGCCCAGCAG CCAGTGCTGC 
AACTGGAATT ACTTTGGCTG GGGAGAACAG AATGACGACC CCGACAGTGC GGTGGATGAC 
CGGGACAGTG ACTATAGGAG TGAGACCAGC AACAGCATCC CACCGCCCTA CTATACGACT 
TCGCAGCCCA ACGCCTCTGT GCACCAGTAC TCCGTGCGAC CGCCCCCTCT GGGGTCCCGG 
GAGTCCTACA GTGACTCCAT GCACAGCTAT GAGGAGTTCT CTGAGCCGCG GGCGCTCAGC 
CCCACAGGCA GCAGCCGCTA TGCTTCTTCC GGGGAGCTGA GCCAGGGAAG CTCACAGCTG 
AGCGAGGACT TCGACCCTGA TGAGCACAGC CTGCAGGGCT CGGAGCTGGA TGACGAGAGG 
GACCGCGATT CTTATCACTC CTGTCACAGC TCCGTGAGTT ACCACAAGGA CTCACCCCGC 
TGGGACCAGG ACGATGAGGA CCTGGAGGAC CTGGAGGACC TGGAAGATGA GGAGCTGCCC 
GAGGAGGAGG AGGAGTTGGA GGAGGAGGGG GAGGAGGAAT TGGAGGAAGA GGATTTGGAG 
GAGGAGGAGG AGGTGCCTGA TGACCTGGCC AGCTACACCC AGCAGGAGGA CACCACTGTG 
GCTGAGCCCA AAGAGTTCAA GCGGATCAGC TTCCCAACGG CTGCGCCTCA GAAGGATGAC 
AAGGTTTCAG CTGTGCCTAC AGAGGCCCCC GAGGTGGCCA AAGGCATCCC CAAAGCAGCC 
ACACCCGAGG AGAAGGCAGC TGCAGAGCGT GCGCAGGAAG CCGAGCCCCC CAAGTCTGAG 
GAGAGTTTCA GATCTCGAGA GGAGGAAGAG GGCCAGGAAG GGCAGGATGC CATGTCCAGA 
GCCAAAGCCA ACTGGTTGCG AGCCTTCAAC AAGGTGCGCA TGCAGCTGCA GGAGGCCCGA 
GGAGAAGGGG ATATGTCTAA GTCTCTATGG TTCAAAGGCG GTCCTGGTGG TGGCCTTATC 
ATCATTGACA GCATGCCAGA CATCAGGAAA CGGAAGCCCA TTCCCCTCGT GAGCGACCTG 
GCTATGTCAT TGGTGCAGTC TCGGAAGGCA GGCATCACCT CGGCCTTGGC CTCCAGCACG 
TTGAACAATG AAGAGCTGAA AAATCACGTT TACAAGAAGA CCCTGCAAGC CTTAATCTAC 
CCCATCTCCT GCACCACGCC GCACAATTTC GAGGTGTGGA CGGCCACCAC GCCCACCTAC 
TGCTATGAGT GCGAGGGGCT GCTGTGGGGC ATCGCGCGGC AGGGCATGCG ATGCACCGAG 
TGCGGCGTTA AGTGCCACGA GAAGTGCCAG GACCTGCTCA ATGCGGATTG CCTGCAGCGG 
GCGGCTGAGA AGAGTTCCAA GCATGGCGCT GAAGACCGCA CGCAGAACAT CATCATGGTG 
CTGAAGGACC GCATGAAGAT CCGCGAGCGC AACAAGCCTG AGATCTTCGA GCTTATCCAA 
GAGATCTTCG CGGTCACCAA GAGCGCACAC ACACAGCAGA TGAAAGCTGT CAAGCAGAGT 
GTGCTGGACG GCACATCCAA GTGGTCTGCC AAAATTAGCA TCACAGTGGT CTGTGCCCAG 
GGCTTGCAGG CCAAGGACAA GACAGGATCC AGTGACCCTT ATGTCACCGT CCAGGTTGGG 
AAGACCAAGA AAAGGACAAA AACCATCTAC GGGAACCTCA ACCCAGTGTG GGAAGAGAAT 
TTTCATTTTG AATGTCACAA CTCCTCTGAC CGGATCAAGG TTCGTGTTTG GGATGAAGAT 
GACGACATAA AATCCCGTGT GAAACAAAGG TTTAAGAGGG AGTCTGATGA CTTCCTAGGG 
CAGACAATCA TCGAGGTGCG GACGCTTAGC GGCGAGATGG ATGTGTGGTA CAATCTGGAC 
AAGAGAACGG ACAAGTCGGC GGTGTCGGGG GCCATCCGGC TTCACATCAG TGTGGAGATC 
AAAGGGGAGG AGAAGGTGGC GCCCTACCAT GTCCAGTACA CCTGTCTGCA TGAGAACCTG 
TTCCACTTTG TGACGGACGT GCAGAACAAT GGTGTGGTGA AGATTCCCGA TGCCAAGGGT 
GACGACGCCT GGAAGGTTTA CTACGATGAG ACGGCCCAGG AGATCGTGGA TGAGTTTGCC 
ATGCGCTATG GTGTTGAGTC CATCTACCAA GCCATGACCC ACTTTGCCTG CCTCTCCTCC 
AAGTATATGT GCCCTGGGGT ACCCGCTGTC ATGAGCACCC TGCTTGCCAA CATCAACGCC 
TACTACGCAC ACACCACCGC CTCCACCAAC GTGTCTGCCT CTGACCGCTT CGCTGCCTCT 
AATTTCGGGA AAGAGCGCTT TGTGAAACTT CTGGACCAGC TACACAATTC TCTGCGGATC 
GACCTGTCCA TGTACCGGAA CAACTTCCCA GCCAGCAGCC CTGAGCGGCT GCAGGATCTC 
AAGTCCACGG TGGACCTGCT CACCAGCATC ACCTTCTTCC GGATGAAGGT TCAGGAACTG 
CAGAGCCCGC CGCGTGCCAG CCAGGTAGTG AAAGACTGTG TGAAGGCCTG CCTCAACTCC 
ACCTATGAGT ACATCTTCAA CAACTGCCAT GAGCTCTATG GCCGGGAGTA CCAAACGGAT 
CCGGCCAAGA AGGGGGAGGT TCCCCCAGAG GAGCAAGGCC CTAGCATCAA GAACCTGGAT 
TTCTGGTCCA AGCTGATCAC CCTCATCGTG TCTATCATCG AGGAGGATAA GAATTCTTAC 
ACACCCTGCC TCAATCAGTT TCCCCAGGAG CTCAATGTGG GGAAGATCAG TGCTGAGGTG 
ATGTGGAGCC TATTTGCCCA AGACATGAAG TACGCCATGG AGGAACATGA CAAACACCGG 
CTGTGTAAGA GCGCAGATTA CATGAACCTG CACTTCAAGG TGAAGTGGCT GTACAATGAG 
TACGTGGCCG AGCTGCCCAC CTTCAAGGAC CGTGTGCCTG AGTACCCTGC GTGGTTTGAG 
CCCTTCGTCA TCCAGTGGTT GGATGAGAAT GAGGAGGTGT CCCGGGACTT CCTGCATGGT 
GCCCTCGAGC GGGACAAGAA GGACGGGTTC CAGCAGACCT CAGAGCACGC CCTGTTCTCT 
TGCTCAGTGG TGGACGTCTT CTCCCAGCTT AACCAGAGTT TCGAGATCAT CAAGAAGCTG 
GAGTGTCCTG ACCCCCAGAT CGTGGGCCAT TACATGCGGC GTTTTGCCAA GACCATTAGC 
AATGTGCTTC TCCAGTATGC AGACATCGTT TCCAAGGACT TCGCCTCCTA CTGCTCCAAG 
GAGAAGGAGA AAGTGCCCTG CATCCTCATG AACAACACAC AGCAACTACG AGTGCAGCTG 
GAGAAGATGT TTGAGGCGAT GGGCGGGAAG GAGCTGGATG CCGAGGCCAG TGGCACCCTG 
AAGGAGCTGC AGGTGAAACT CAACAATGTC CTGGATGAAC TCAGCCATGT GTTTGCCACC 
AGCTTCCAGC CACACATTGA GGAATGCGTC AGACAGATGG GTGACATCCT GAGCCAGGTG 
AAGGGCACAG GCAATGTACC TGCCAGTGCC TGCAGCAGCG TGGCCCAGGA TGCAGACAAT 
GTGCTGCAGC CCATCATGGA TCTTCTGGAC AGCAACCTCA CCCTGTTTGC CAAAATCTGT 
GAGAAGACAG TTCTGAAGCG GGTGCTGAAG GAGCTCTGGA AGCTGGTGAT GAACACAATG 
GAGAAGACCA TCGTCCTGCC ACCGCTCACT GACCAGACGA TGATTGGGAC ACAAATGATC 
TTCAATGCTG CCAAGGAGCT GGGCCAGCTG TCCAAACTTA AGGATCACAT GGTGCGGGAA 
GAAGCCAAGA GCTTGACCCC GAAGCAGTGT GCTGTTGTTG AACTGGCCCT GGACACCATC 
AAGCAATACT TCCACGCGGG GGGCGTTGGC CTCAAGAAGA CCTTCCTGGA GAAGAGCCCG 
GACCTCCAGT CCCTGCGCTA CGCCCTGTCG CTCTACACGC AAGCCACCGA CCTGCTCATC 
AAAACCTTCG TGCAGACGCA GTCAGCGCAG GGCTCTGGTG TGGAGGACCC TGTAGGTGAA 
GTGTCCGTCC ACGTGGAGCT GTTCACACAT CCAGGAACTG GGGAACAGAA GGTCACAGTG 
AAGGTGGTGG CCGCCAATGA CCTCAAGTGG CAGACTTCTG GCATCTTCCG CCCGTTCATC 
GAGGTCAACA TCGTTGGACC TCAGCTTAGC GACAAGAAAC GCAAGTTCGC CACCAAATCC 
AAAAACAACA GCTGGGCGCC CAAGTATAAC GAGAGCTTCC AGTTCTCCCT GAGCGCCGAC 
GCGGGCCCCG AGTGCTACGA GCTGCAGGTG TGCGTGAAGG ACTACTGCTT TGCGCGCGAA 
GACCGCACGG TGGGGCTGGC GGTGCTGCAA CTGCGAGAGC TGGCCCAGCG CGGGAGCGCC 
GCGTGCTGGC TTCCACTCGG CCGCCGCATC CACATGGACG ACACGGGGCT CACAGTGCTG 
CGCATCCTGT CGCAGCGCAG CAACGATGAG GTGGCCAAGG AATTCGTCAA GCTCAAGTCG 
GACACGCGCT CAGCCGAGGA GGGCGGTGCC GCGCCTGCGC CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168369.1
CDS	125..5209
Translation	MSLLCVGVKKAKFDGAQEKFNTYVTLKVQNVKSTTIAVRGSQPSWEQDFMFEINRLDLGLTVEVWNKGLIWDTMVGTVWIPLRTIRQSNEEGPGEWLTLDSQAIMADSEICGTKDPTFHRILLDAHFELPLDIPEEEARYWAKKLEQLNAMRDQDEVSHLQYSFQDQQDKPLPVPSSQCCNWNYFGWGEQNDDPDSAVDDRDSDYRSETSNSIPPPYYTTSQPNASVHQYSVRPPPLGSRESYSDSMHSYEEFSEPRALSPTGSSRYASSGELSQGSSQLSEDFDPDEHSLQGSELDDERDRDSYHSCHSSVSYHKDSPRWDQDDEDLEDLEDLEDEELPEEEEELEEEGEEELEEEDLEEEEEVPDDLASYTQQEDTTVAEPKEFKRISFPTAAPQKDDKVSAVPTEAPEVAKGIPKAATPEEKAAAERAQEAEPPKSEESFRSREEEEGQEGQDAMSRAKANWLRAFNKVRMQLQEARGEGDMSKSLWFKGGPGGGLIIIDSMPDIRKRKPIPLVSDLAMSLVQSRKAGITSALASSTLNNEELKNHVYKKTLQALIYPISCTTPHNFEVWTATTPTYCYECEGLLWGIARQGMRCTECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMVLKDRMKIRERNKPEIFELIQEIFAVTKSAHTQQMKAVKQSVLDGTSKWSAKISITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIYGNLNPVWEENFHFECHNSSDRIKVRVWDEDDDIKSRVKQRFKRESDDFLGQTIIEVRTLSGEMDVWYNLDKRTDKSAVSGAIRLHISVEIKGEEKVAPYHVQYTCLHENLFHFVTDVQNNGVVKIPDAKGDDAWKVYYDETAQEIVDEFAMRYGVESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSMYRNNFPASSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHELYGREYQTDPAKKGEVPPEEQGPSIKNLDFWSKLITLIVSIIEEDKNSYTPCLNQFPQELNVGKISAEVMWSLFAQDMKYAMEEHDKHRLCKSADYMNLHFKVKWLYNEYVAELPTFKDRVPEYPAWFEPFVIQWLDENEEVSRDFLHGALERDKKDGFQQTSEHALFSCSVVDVFSQLNQSFEIIKKLECPDPQIVGHYMRRFAKTISNVLLQYADIVSKDFASYCSKEKEKVPCILMNNTQQLRVQLEKMFEAMGGKELDAEASGTLKELQVKLNNVLDELSHVFATSFQPHIEECVRQMGDILSQVKGTGNVPASACSSVAQDADNVLQPIMDLLDSNLTLFAKICEKTVLKRVLKELWKLVMNTMEKTIVLPPLTDQTMIGTQMIFNAAKELGQLSKLKDHMVREEAKSLTPKQCAVVELALDTIKQYFHAGGVGLKKTFLEKSPDLQSLRYALSLYTQATDLLIKTFVQTQSAQGSGVEDPVGEVSVHVELFTHPGTGEQKVTVKVVAANDLKWQTSGIFRPFIEVNIVGPQLSDKKRKFATKSKNNSWAPKYNESFQFSLSADAGPECYELQVCVKDYCFAREDRTVGLAVLQLRELAQRGSAACWLPLGRRIHMDDTGLTVLRILSQRSNDEVAKEFVKLKSDTRSAEEGGAAPAP

Target ORF information:

RefSeq Version	XM_017312880.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus unc-13 homolog A (C. elegans) (Unc13a), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312880.1

ORF Insert Sequence:

ATGTCTCTGC TGTGCGTGGG AGTCAAAAAA GCCAAGTTTG ACGGTGCCCA AGAGAAGTTC 
AACACATACG TGACGCTGAA GGTGCAGAAC GTGAAGAGCA CTACCATAGC TGTACGTGGC 
AGCCAGCCCA GCTGGGAGCA GGACTTCATG TTTGAGATCA ACCGCCTGGA TCTAGGCCTG 
ACCGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGTAC TGTGTGGATC 
CCGCTGCGGA CCATCCGCCA GTCCAATGAG GAGGGCCCAG GAGAGTGGCT CACACTGGAC 
TCTCAGGCCA TCATGGCGGA CAGTGAGATC TGTGGGACCA AGGACCCCAC CTTCCATCGC 
ATCCTCCTGG ACGCTCATTT CGAGCTGCCG TTGGACATCC CTGAGGAGGA GGCACGCTAC 
TGGGCCAAGA AGCTGGAGCA GCTGAATGCC ATGCGTGACC AGGATGAGGT GAGTCACCTC 
CAGTACTCCT TCCAGGACCA GCAGGACAAG CCGCTGCCAG TGCCCAGCAG CCAGTGCTGC 
AACTGGAATT ACTTTGGCTG GGGAGAACAG AATGACGACC CCGACAGTGC GGTGGATGAC 
CGGGACAGTG ACTATAGGAG TGAGACCAGC AACAGCATCC CACCGCCCTA CTATACGACT 
TCGCAGCCCA ACGCCTCTGT GCACCAGTAC TCCGTGCGAC CGCCCCCTCT GGGGTCCCGG 
GAGTCCTACA GTGACTCCAT GCACAGCTAT GAGGAGTTCT CTGAGCCGCG GGCGCTCAGC 
CCCACAGGCA GCAGCCGCTA TGCTTCTTCC GGGGAGCTGA GCCAGGGAAG CTCACAGCTG 
AGCGAGGACT TCGACCCTGA TGAGCACAGC CTGCAGGGCT CGGAGCTGGA TGACGAGAGG 
GACCGCGATT CTTATCACTC CTGTCACAGC TCCGTGAGTT ACCACAAGGA CTCACCCCGC 
TGGGACCAGG ACGATGAGGA CCTGGAGGAC CTGGAGGACC TGGAAGATGA GGAGCTGCCC 
GAGGAGGAGG AGGAGTTGGA GGAGGAGGGG GAGGAGGAAT TGGAGGAAGA GGATTTGGAG 
GAGGAGGAGG AGGTGCCTGA TGACCTGGCC AGCTACACCC AGCAGGAGGA CACCACTGTG 
GCTGAGCCCA AAGAGTTCAA GCGGATCAGC TTCCCAACGG CTGCGCCTCA GAAGGATGAC 
AAGGTTTCAG CTGTGCCTAC AGAGGCCCCC GAGGTGGCCA AAGGCATCCC CAAAGCAGCC 
ACACCCGAGG AGAAGGCAGC TGCAGAGCGT GCGCAGGAAG CCGAGCCCCC CAAGTCTGAG 
GAGAGTTTCA GATCTCGAGA GGAGGAAGAG GGCCAGGAAG GGCAGGATGC CATGTCCAGA 
GCCAAAGCCA ACTGGTTGCG AGCCTTCAAC AAGGTGCGCA TGCAGCTGCA GGAGGCCCGA 
GGAGAAGGGG ATATGTCTAA GTCTCTATGG TTCAAAGGCG GTCCTGGTGG TGGCCTTATC 
ATCATTGACA GCATGCCAGA CATCAGGAAA CGGAAGCCCA TTCCCCTCGT GAGCGACCTG 
GCTATGTCAT TGGTGCAGTC TCGGAAGGCA GGCATCACCT CGGCCTTGGC CTCCAGCACG 
TTGAACAATG AAGAGCTGAA AAATCACGTT TACAAGAAGA CCCTGCAAGC CTTAATCTAC 
CCCATCTCCT GCACCACGCC GCACAATTTC GAGGTGTGGA CGGCCACCAC GCCCACCTAC 
TGCTATGAGT GCGAGGGGCT GCTGTGGGGC ATCGCGCGGC AGGGCATGCG ATGCACCGAG 
TGCGGCGTTA AGTGCCACGA GAAGTGCCAG GACCTGCTCA ATGCGGATTG CCTGCAGCGG 
GCGGCTGAGA AGAGTTCCAA GCATGGCGCT GAAGACCGCA CGCAGAACAT CATCATGGTG 
CTGAAGGACC GCATGAAGAT CCGCGAGCGC AACAAGCCTG AGATCTTCGA GCTTATCCAA 
GAGATCTTCG CGGTCACCAA GAGCGCACAC ACACAGCAGA TGAAAGCTGT CAAGCAGAGT 
GTGCTGGACG GCACATCCAA GTGGTCTGCC AAAATTAGCA TCACAGTGGT CTGTGCCCAG 
GGCTTGCAGG CCAAGGACAA GACAGGATCC AGTGACCCTT ATGTCACCGT CCAGGTTGGG 
AAGACCAAGA AAAGGACAAA AACCATCTAC GGGAACCTCA ACCCAGTGTG GGAAGAGAAT 
TTTCATTTTG AATGTCACAA CTCCTCTGAC CGGATCAAGG TTCGTGTTTG GGATGAAGAT 
GACGACATAA AATCCCGTGT GAAACAAAGG TTTAAGAGGG AGTCTGATGA CTTCCTAGGG 
CAGACAATCA TCGAGGTGCG GACGCTTAGC GGCGAGATGG ATGTGTGGTA CAATCTGGAC 
AAGAGAACGG ACAAGTCGGC GGTGTCGGGG GCCATCCGGC TTCACATCAG TGTGGAGATC 
AAAGGGGAGG AGAAGGTGGC GCCCTACCAT GTCCAGTACA CCTGTCTGCA TGAGAACCTG 
TTCCACTTTG TGACGGACGT GCAGAACAAT GGTGTGGTGA AGATTCCCGA TGCCAAGGGT 
GACGACGCCT GGAAGGTTTA CTACGATGAG ACGGCCCAGG AGATCGTGGA TGAGTTTGCC 
ATGCGCTATG GTGTTGAGTC CATCTACCAA GCCATGACCC ACTTTGCCTG CCTCTCCTCC 
AAGTATATGT GCCCTGGGGT ACCCGCTGTC ATGAGCACCC TGCTTGCCAA CATCAACGCC 
TACTACGCAC ACACCACCGC CTCCACCAAC GTGTCTGCCT CTGACCGCTT CGCTGCCTCT 
AATTTCGGGA AAGAGCGCTT TGTGAAACTT CTGGACCAGC TACACAATTC TCTGCGGATC 
GACCTGTCCA TGTACCGGAA CAACTTCCCA GCCAGCAGCC CTGAGCGGCT GCAGGATCTC 
AAGTCCACGG TGGACCTGCT CACCAGCATC ACCTTCTTCC GGATGAAGGT TCAGGAACTG 
CAGAGCCCGC CGCGTGCCAG CCAGGTAGTG AAAGACTGTG TGAAGGCCTG CCTCAACTCC 
ACCTATGAGT ACATCTTCAA CAACTGCCAT GAGCTCTATG GCCGGGAGTA CCAAACGGAT 
CCGGCCAAGA AGGGGGAGGT TCCCCCAGAG GAGCAAGGCC CTAGCATCAA GAACCTGGAT 
TTCTGGTCCA AGCTGATCAC CCTCATCGTG TCTATCATCG AGGAGGATAA GAATTCTTAC 
ACACCCTGCC TCAATCAGTT TCCCCAGGAG CTCAATGTGG GGAAGATCAG TGCTGAGGTG 
ATGTGGAGCC TATTTGCCCA AGACATGAAG TACGCCATGG AGGAACATGA CAAACACCGG 
CTGTGTAAGA GCGCAGATTA CATGAACCTG CACTTCAAGG TGAAGTGGCT GTACAATGAG 
TACGTGGCCG AGCTGCCCAC CTTCAAGGAC CGTGTGCCTG AGTACCCTGC GTGGTTTGAG 
CCCTTCGTCA TCCAGTGGTT GGATGAGAAT GAGGAGGTGT CCCGGGACTT CCTGCATGGT 
GCCCTCGAGC GGGACAAGAA GGACGGGTTC CAGCAGACCT CAGAGCACGC CCTGTTCTCT 
TGCTCAGTGG TGGACGTCTT CTCCCAGCTT AACCAGAGTT TCGAGATCAT CAAGAAGCTG 
GAGTGTCCTG ACCCCCAGAT CGTGGGCCAT TACATGCGGC GTTTTGCCAA GACCATTAGC 
AATGTGCTTC TCCAGTATGC AGACATCGTT TCCAAGGACT TCGCCTCCTA CTGCTCCAAG 
GAGAAGGAGA AAGTGCCCTG CATCCTCATG AACAACACAC AGCAACTACG AGTGCAGCTG 
GAGAAGATGT TTGAGGCGAT GGGCGGGAAG GAGCTGGATG CCGAGGCCAG TGGCACCCTG 
AAGGAGCTGC AGGTGAAACT CAACAATGTC CTGGATGAAC TCAGCCATGT GTTTGCCACC 
AGCTTCCAGC CACACATTGA GGAATGCGTC AGACAGATGG GTGACATCCT GAGCCAGGTG 
AAGGGCACAG GCAATGTACC TGCCAGTGCC TGCAGCAGCG TGGCCCAGGA TGCAGACAAT 
GTGCTGCAGC CCATCATGGA TCTTCTGGAC AGCAACCTCA CCCTGTTTGC CAAAATCTGT 
GAGAAGACAG TTCTGAAGCG GGTGCTGAAG GAGCTCTGGA AGCTGGTGAT GAACACAATG 
GAGAAGACCA TCGTCCTGCC ACCGCTCACT GACCAGACGA TGATTGGGAC ACAAATGATC 
TTCAATGCTG CCAAGGAGCT GGGCCAGCTG TCCAAACTTA AGGATCACAT GGTGCGGGAA 
GAAGCCAAGA GCTTGACCCC GAAGCAGTGT GCTGTTGTTG AACTGGCCCT GGACACCATC 
AAGCAATACT TCCACGCGGG GGGCGTTGGC CTCAAGAAGA CCTTCCTGGA GAAGAGCCCG 
GACCTCCAGT CCCTGCGCTA CGCCCTGTCG CTCTACACGC AAGCCACCGA CCTGCTCATC 
AAAACCTTCG TGCAGACGCA GTCAGCGCAG GGCTCTGGTG TGGAGGACCC TGTAGGTGAA 
GTGTCCGTCC ACGTGGAGCT GTTCACACAT CCAGGAACTG GGGAACAGAA GGTCACAGTG 
AAGGTGGTGG CCGCCAATGA CCTCAAGTGG CAGACTTCTG GCATCTTCCG CCCGTTCATC 
GAGGTCAACA TCGTTGGACC TCAGCTTAGC GACAAGAAAC GCAAGTTCGC CACCAAATCC 
AAAAACAACA GCTGGGCGCC CAAGTATAAC GAGAGCTTCC AGTTCTCCCT GAGCGCCGAC 
GCGGGCCCCG AGTGCTACGA GCTGCAGGTG TGCGTGAAGG ACTACTGCTT TGCGCGCGAA 
GACCGCACGG TGGGGCTGGC GGTGCTGCAA CTGCGAGAGC TGGCCCAGCG CGGGAGCGCC 
GCGTGCTGGC TTCCACTCGG CCGCCGCATC CACATGGACG ACACGGGGCT CACAGTGCTG 
CGCATCCTGT CGCAGCGCAG CAACGATGAG GTGGCCAAGG AATTCGTCAA GCTCAAGTCG 
GACACGCGCT CAGCCGAGGA GGGCGGTGCC GCGCCTGCGC CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Unc13a ( XM_017312881.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168370.1 Mus musculus unc-13 homolog A (C. elegans) (Unc13a), transcript variant X9, mRNA.

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CloneID	OMu82531
Clone ID Related Accession (Same CDS sequence)	XM_017312881.2 , XM_017312881.1
Accession Version	XM_017312881.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5079bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein unc-13 homolog A isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTGC TGTGCGTGGG AGTCAAAAAA GCCAAGTTTG ACGGTGCCCA AGAGAAGTTC 
AACACATACG TGACGCTGAA GGTGCAGAAC GTGAAGAGCA CTACCATAGC TGTACGTGGC 
AGCCAGCCCA GCTGGGAGCA GGACTTCATG TTTGAGATCA ACCGCCTGGA TCTAGGCCTG 
ACCGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGTAC TGTGTGGATC 
CCGCTGCGGA CCATCCGCCA GTCCAATGAG GAGGGCCCAG GAGAGTGGCT CACACTGGAC 
TCTCAGGCCA TCATGGCGGA CAGTGAGATC TGTGGGACCA AGGACCCCAC CTTCCATCGC 
ATCCTCCTGG ACGCTCATTT CGAGCTGCCG TTGGACATCC CTGAGGAGGA GGCACGCTAC 
TGGGCCAAGA AGCTGGAGCA GCTGAATGCC ATGCGTGACC AGGATGAGGT GAGTCACCTC 
CAGTACTCCT TCCAGGACCA GCAGGACAAG CCGCTGCCAG TGCCCAGCAG CCAGTGCTGC 
AACTGGAATT ACTTTGGCTG GGGAGAACAG AATGACGACC CCGACAGTGC GGTGGATGAC 
CGGGACAGTG ACTATAGGAG TGAGACCAGC AACAGCATCC CACCGCCCTA CTATACGACT 
TCGCAGCCCA ACGCCTCTGT GCACCAGTAC TCCGTGCGAC CGCCCCCTCT GGGGTCCCGG 
GAGTCCTACA GTGACTCCAT GCACAGCTAT GAGGAGTTCT CTGAGCCGCG GGCGCTCAGC 
CCCACAGGCA GCAGCCGCTA TGCTTCTTCC GGGGAGCTGA GCCAGGGAAG CTCACAGCTG 
AGCGAGGACT TCGACCCTGA TGAGCACAGC CTGCAGGGCT CGGAGCTGGA TGACGAGAGG 
GACCGCGATT CTTATCACTC CTGTCACAGC TCCGTGAGTT ACCACAAGGA CTCACCCCGC 
TGGGACCAGG ACGATGAGGA CCTGGAGGAC CTGGAGGACC TGGAAGATGA GGAGCTGCCC 
GAGGAGGAGG AGGAGTTGGA GGAGGAGGGG GAGGAGGAAT TGGAGGAAGA GGATTTGGAG 
GAGGAGGAGG AGGTGCCTGA TGACCTGGCC AGCTACACCC AGCAGGAGGA CACCACTGTG 
GCTGAGCCCA AAGAGTTCAA GCGGATCAGC TTCCCAACGG CTGCGCCTCA GAAGGATGAC 
AAGGTTTCAG CTGTGCCTAC AGAGGCCCCC GAGGTGGCCA AAGGCATCCC CAAAGCAGCC 
ACACCCGAGG AGAAGGCAGC TGCAGAGCGT GCGCAGGAAG CCGAGCCCCC CAAGTCTGAG 
GAGAGTTTCA GATCTCGAGA GGAGGAAGAG GGCCAGGAAG GGCAGGATGC CATGTCCAGA 
GCCAAAGCCA ACTGGTTGCG AGCCTTCAAC AAGGTGCGCA TGCAGCTGCA GGAGGCCCGA 
GGAGAAGGGG ATATGTCTAA GTCTCTATGG TTCAAAGGCG GTCCTGGTGG TGGCCTTATC 
ATCATTGACA GCATGCCAGA CATCAGGAAA CGGAAGCCCA TTCCCCTCGT GAGCGACCTG 
GCTATGTCAT TGGTGCAGTC TCGGAAGGCA GGCATCACCT CGGCCTTGGC CTCCAGCACG 
TTGAACAATG AAGAGCTGAA AAATCACGTT TACAAGAAGA CCCTGCAAGC CTTAATCTAC 
CCCATCTCCT GCACCACGCC GCACAATTTC GAGGTGTGGA CGGCCACCAC GCCCACCTAC 
TGCTATGAGT GCGAGGGGCT GCTGTGGGGC ATCGCGCGGC AGGGCATGCG ATGCACCGAG 
TGCGGCGTTA AGTGCCACGA GAAGTGCCAG GACCTGCTCA ATGCGGATTG CCTGCAGCGG 
GCGGCTGAGA AGAGTTCCAA GCATGGCGCT GAAGACCGCA CGCAGAACAT CATCATGGTG 
CTGAAGGACC GCATGAAGAT CCGCGAGCGC AACAAGCCTG AGATCTTCGA GCTTATCCAA 
GAGATCTTCG CGGTCACCAA GAGCGCACAC ACACAGCAGA TGAAAGCTGT CAAGCAGAGT 
GTGCTGGACG GCACATCCAA GTGGTCTGCC AAAATTAGCA TCACAGTGGT CTGTGCCCAG 
GGCTTGCAGG CCAAGGACAA GACAGGATCC AGTGACCCTT ATGTCACCGT CCAGGTTGGG 
AAGACCAAGA AAAGGACAAA AACCATCTAC GGGAACCTCA ACCCAGTGTG GGAAGAGAAT 
TTTCATTTTG AATGTCACAA CTCCTCTGAC CGGATCAAGG TTCGTGTTTG GGATGAAGAT 
GACGACATAA AATCCCGTGT GAAACAAAGG TTTAAGAGGG AGTCTGATGA CTTCCTAGGG 
CAGACAATCA TCGAGGTGCG GACGCTTAGC GGCGAGATGG ATGTGTGGTA CAATCTGGAC 
AAGAGAACGG ACAAGTCGGC GGTGTCGGGG GCCATCCGGC TTCACATCAG TGTGGAGATC 
AAAGGGGAGG AGAAGGTGGC GCCCTACCAT GTCCAGTACA CCTGTCTGCA TGAGAACCTG 
TTCCACTTTG TGACGGACGT GCAGAACAAT GGTGTGGTGA AGATTCCCGA TGCCAAGGGT 
GACGACGCCT GGAAGGTTTA CTACGATGAG ACGGCCCAGG AGATCGTGGA TGAGTTTGCC 
ATGCGCTATG GTGTTGAGTC CATCTACCAA GCCATGACCC ACTTTGCCTG CCTCTCCTCC 
AAGTATATGT GCCCTGGGGT ACCCGCTGTC ATGAGCACCC TGCTTGCCAA CATCAACGCC 
TACTACGCAC ACACCACCGC CTCCACCAAC GTGTCTGCCT CTGACCGCTT CGCTGCCTCT 
AATTTCGGGA AAGAGCGCTT TGTGAAACTT CTGGACCAGC TACACAATTC TCTGCGGATC 
GACCTGTCCA TGTACCGGAA CAACTTCCCA GCCAGCAGCC CTGAGCGGCT GCAGGATCTC 
AAGTCCACGG TGGACCTGCT CACCAGCATC ACCTTCTTCC GGATGAAGGT TCAGGAACTG 
CAGAGCCCGC CGCGTGCCAG CCAGGTAGTG AAAGACTGTG TGAAGGCCTG CCTCAACTCC 
ACCTATGAGT ACATCTTCAA CAACTGCCAT GAGCTCTATG GCCGGGAGTA CCAAACGGAT 
CCGGCCAAGA AGGGGGAGGT TCCCCCAGAG GAGCAAGGCC CTAGCATCAA GAACCTGGAT 
TTCTGGTCCA AGCTGATCAC CCTCATCGTG TCTATCATCG AGGAGGATAA GAATTCTTAC 
ACACCCTGCC TCAATCAGTT TCCCCAGGAG CTCAATGTGG GGAAGATCAG TGCTGAGGTG 
ATGTGGAGCC TATTTGCCCA AGACATGAAG TACGCCATGG AGGAACATGA CAAACACCGG 
CTGTGTAAGA GCGCAGATTA CATGAACCTG CACTTCAAGG TGAAGTGGCT GTACAATGAG 
TACGTGGCCG AGCTGCCCAC CTTCAAGGAC CGTGTGCCTG AGTACCCTGC GTGGTTTGAG 
CCCTTCGTCA TCCAGTGGTT GGATGAGAAT GAGGAGGTGT CCCGGGACTT CCTGCATGGT 
GCCCTCGAGC GGGACAAGAA GGACGGGTTC CAGCAGACCT CAGAGCACGC CCTGTTCTCT 
TGCTCAGTGG TGGACGTCTT CTCCCAGCTT AACCAGAGTT TCGAGATCAT CAAGAAGCTG 
GAGTGTCCTG ACCCCCAGAT CGTGGGCCAT TACATGCGGC GTTTTGCCAA GACCATTAGC 
AATGTGCTTC TCCAGTATGC AGACATCGTT TCCAAGGACT TCGCCTCCTA CTGCTCCAAG 
GAGAAGGAGA AAGTGCCCTG CATCCTCATG AACAACACAC AGCAACTACG AGTGCAGCTG 
GAGAAGATGT TTGAGGCGAT GGGCGGGAAG GAGCTGGATG CCGAGGCCAG TGGCACCCTG 
AAGGAGCTGC AGGTGAAACT CAACAATGTC CTGGATGAAC TCAGCCATGT GTTTGCCACC 
AGCTTCCAGC CACACATTGA GGAATGCGTC AGACAGATGG GTGACATCCT GAGCCAGGTG 
AAGGGCACAG GCAATGTACC TGCCAGTGCC TGCAGCAGCG TGGCCCAGGA TGCAGACAAT 
GTGCTGCAGC CCATCATGGA TCTTCTGGAC AGCAACCTCA CCCTGTTTGC CAAAATCTGT 
GAGAAGACAG TTCTGAAGCG GGTGCTGAAG GAGCTCTGGA AGCTGGTGAT GAACACAATG 
GAGAAGACCA TCGTCCTGCC ACCGCTCACT GACCAGACGG GGACACAAAT GATCTTCAAT 
GCTGCCAAGG AGCTGGGCCA GCTGTCCAAA CTTAAGGATC ACATGGTGCG GGAAGAAGCC 
AAGAGCTTGA CCCCGAAGCA GTGTGCTGTT GTTGAACTGG CCCTGGACAC CATCAAGCAA 
TACTTCCACG CGGGGGGCGT TGGCCTCAAG AAGACCTTCC TGGAGAAGAG CCCGGACCTC 
CAGTCCCTGC GCTACGCCCT GTCGCTCTAC ACGCAAGCCA CCGACCTGCT CATCAAAACC 
TTCGTGCAGA CGCAGTCAGC GCAGGGCTCT GGTGTGGAGG ACCCTGTAGG TGAAGTGTCC 
GTCCACGTGG AGCTGTTCAC ACATCCAGGA ACTGGGGAAC AGAAGGTCAC AGTGAAGGTG 
GTGGCCGCCA ATGACCTCAA GTGGCAGACT TCTGGCATCT TCCGCCCGTT CATCGAGGTC 
AACATCGTTG GACCTCAGCT TAGCGACAAG AAACGCAAGT TCGCCACCAA ATCCAAAAAC 
AACAGCTGGG CGCCCAAGTA TAACGAGAGC TTCCAGTTCT CCCTGAGCGC CGACGCGGGC 
CCCGAGTGCT ACGAGCTGCA GGTGTGCGTG AAGGACTACT GCTTTGCGCG CGAAGACCGC 
ACGGTGGGGC TGGCGGTGCT GCAACTGCGA GAGCTGGCCC AGCGCGGGAG CGCCGCGTGC 
TGGCTTCCAC TCGGCCGCCG CATCCACATG GACGACACGG GGCTCACAGT GCTGCGCATC 
CTGTCGCAGC GCAGCAACGA TGAGGTGGCC AAGGAATTCG TCAAGCTCAA GTCGGACACG 
CGCTCAGCCG AGGAGGGCGG TGCCGCGCCT GCGCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168370.1
CDS	125..5203
Translation	MSLLCVGVKKAKFDGAQEKFNTYVTLKVQNVKSTTIAVRGSQPSWEQDFMFEINRLDLGLTVEVWNKGLIWDTMVGTVWIPLRTIRQSNEEGPGEWLTLDSQAIMADSEICGTKDPTFHRILLDAHFELPLDIPEEEARYWAKKLEQLNAMRDQDEVSHLQYSFQDQQDKPLPVPSSQCCNWNYFGWGEQNDDPDSAVDDRDSDYRSETSNSIPPPYYTTSQPNASVHQYSVRPPPLGSRESYSDSMHSYEEFSEPRALSPTGSSRYASSGELSQGSSQLSEDFDPDEHSLQGSELDDERDRDSYHSCHSSVSYHKDSPRWDQDDEDLEDLEDLEDEELPEEEEELEEEGEEELEEEDLEEEEEVPDDLASYTQQEDTTVAEPKEFKRISFPTAAPQKDDKVSAVPTEAPEVAKGIPKAATPEEKAAAERAQEAEPPKSEESFRSREEEEGQEGQDAMSRAKANWLRAFNKVRMQLQEARGEGDMSKSLWFKGGPGGGLIIIDSMPDIRKRKPIPLVSDLAMSLVQSRKAGITSALASSTLNNEELKNHVYKKTLQALIYPISCTTPHNFEVWTATTPTYCYECEGLLWGIARQGMRCTECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMVLKDRMKIRERNKPEIFELIQEIFAVTKSAHTQQMKAVKQSVLDGTSKWSAKISITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIYGNLNPVWEENFHFECHNSSDRIKVRVWDEDDDIKSRVKQRFKRESDDFLGQTIIEVRTLSGEMDVWYNLDKRTDKSAVSGAIRLHISVEIKGEEKVAPYHVQYTCLHENLFHFVTDVQNNGVVKIPDAKGDDAWKVYYDETAQEIVDEFAMRYGVESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSMYRNNFPASSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHELYGREYQTDPAKKGEVPPEEQGPSIKNLDFWSKLITLIVSIIEEDKNSYTPCLNQFPQELNVGKISAEVMWSLFAQDMKYAMEEHDKHRLCKSADYMNLHFKVKWLYNEYVAELPTFKDRVPEYPAWFEPFVIQWLDENEEVSRDFLHGALERDKKDGFQQTSEHALFSCSVVDVFSQLNQSFEIIKKLECPDPQIVGHYMRRFAKTISNVLLQYADIVSKDFASYCSKEKEKVPCILMNNTQQLRVQLEKMFEAMGGKELDAEASGTLKELQVKLNNVLDELSHVFATSFQPHIEECVRQMGDILSQVKGTGNVPASACSSVAQDADNVLQPIMDLLDSNLTLFAKICEKTVLKRVLKELWKLVMNTMEKTIVLPPLTDQTGTQMIFNAAKELGQLSKLKDHMVREEAKSLTPKQCAVVELALDTIKQYFHAGGVGLKKTFLEKSPDLQSLRYALSLYTQATDLLIKTFVQTQSAQGSGVEDPVGEVSVHVELFTHPGTGEQKVTVKVVAANDLKWQTSGIFRPFIEVNIVGPQLSDKKRKFATKSKNNSWAPKYNESFQFSLSADAGPECYELQVCVKDYCFAREDRTVGLAVLQLRELAQRGSAACWLPLGRRIHMDDTGLTVLRILSQRSNDEVAKEFVKLKSDTRSAEEGGAAPAP

Target ORF information:

RefSeq Version	XM_017312881.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus unc-13 homolog A (C. elegans) (Unc13a), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312881.1

ORF Insert Sequence:

ATGTCTCTGC TGTGCGTGGG AGTCAAAAAA GCCAAGTTTG ACGGTGCCCA AGAGAAGTTC 
AACACATACG TGACGCTGAA GGTGCAGAAC GTGAAGAGCA CTACCATAGC TGTACGTGGC 
AGCCAGCCCA GCTGGGAGCA GGACTTCATG TTTGAGATCA ACCGCCTGGA TCTAGGCCTG 
ACCGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGTAC TGTGTGGATC 
CCGCTGCGGA CCATCCGCCA GTCCAATGAG GAGGGCCCAG GAGAGTGGCT CACACTGGAC 
TCTCAGGCCA TCATGGCGGA CAGTGAGATC TGTGGGACCA AGGACCCCAC CTTCCATCGC 
ATCCTCCTGG ACGCTCATTT CGAGCTGCCG TTGGACATCC CTGAGGAGGA GGCACGCTAC 
TGGGCCAAGA AGCTGGAGCA GCTGAATGCC ATGCGTGACC AGGATGAGGT GAGTCACCTC 
CAGTACTCCT TCCAGGACCA GCAGGACAAG CCGCTGCCAG TGCCCAGCAG CCAGTGCTGC 
AACTGGAATT ACTTTGGCTG GGGAGAACAG AATGACGACC CCGACAGTGC GGTGGATGAC 
CGGGACAGTG ACTATAGGAG TGAGACCAGC AACAGCATCC CACCGCCCTA CTATACGACT 
TCGCAGCCCA ACGCCTCTGT GCACCAGTAC TCCGTGCGAC CGCCCCCTCT GGGGTCCCGG 
GAGTCCTACA GTGACTCCAT GCACAGCTAT GAGGAGTTCT CTGAGCCGCG GGCGCTCAGC 
CCCACAGGCA GCAGCCGCTA TGCTTCTTCC GGGGAGCTGA GCCAGGGAAG CTCACAGCTG 
AGCGAGGACT TCGACCCTGA TGAGCACAGC CTGCAGGGCT CGGAGCTGGA TGACGAGAGG 
GACCGCGATT CTTATCACTC CTGTCACAGC TCCGTGAGTT ACCACAAGGA CTCACCCCGC 
TGGGACCAGG ACGATGAGGA CCTGGAGGAC CTGGAGGACC TGGAAGATGA GGAGCTGCCC 
GAGGAGGAGG AGGAGTTGGA GGAGGAGGGG GAGGAGGAAT TGGAGGAAGA GGATTTGGAG 
GAGGAGGAGG AGGTGCCTGA TGACCTGGCC AGCTACACCC AGCAGGAGGA CACCACTGTG 
GCTGAGCCCA AAGAGTTCAA GCGGATCAGC TTCCCAACGG CTGCGCCTCA GAAGGATGAC 
AAGGTTTCAG CTGTGCCTAC AGAGGCCCCC GAGGTGGCCA AAGGCATCCC CAAAGCAGCC 
ACACCCGAGG AGAAGGCAGC TGCAGAGCGT GCGCAGGAAG CCGAGCCCCC CAAGTCTGAG 
GAGAGTTTCA GATCTCGAGA GGAGGAAGAG GGCCAGGAAG GGCAGGATGC CATGTCCAGA 
GCCAAAGCCA ACTGGTTGCG AGCCTTCAAC AAGGTGCGCA TGCAGCTGCA GGAGGCCCGA 
GGAGAAGGGG ATATGTCTAA GTCTCTATGG TTCAAAGGCG GTCCTGGTGG TGGCCTTATC 
ATCATTGACA GCATGCCAGA CATCAGGAAA CGGAAGCCCA TTCCCCTCGT GAGCGACCTG 
GCTATGTCAT TGGTGCAGTC TCGGAAGGCA GGCATCACCT CGGCCTTGGC CTCCAGCACG 
TTGAACAATG AAGAGCTGAA AAATCACGTT TACAAGAAGA CCCTGCAAGC CTTAATCTAC 
CCCATCTCCT GCACCACGCC GCACAATTTC GAGGTGTGGA CGGCCACCAC GCCCACCTAC 
TGCTATGAGT GCGAGGGGCT GCTGTGGGGC ATCGCGCGGC AGGGCATGCG ATGCACCGAG 
TGCGGCGTTA AGTGCCACGA GAAGTGCCAG GACCTGCTCA ATGCGGATTG CCTGCAGCGG 
GCGGCTGAGA AGAGTTCCAA GCATGGCGCT GAAGACCGCA CGCAGAACAT CATCATGGTG 
CTGAAGGACC GCATGAAGAT CCGCGAGCGC AACAAGCCTG AGATCTTCGA GCTTATCCAA 
GAGATCTTCG CGGTCACCAA GAGCGCACAC ACACAGCAGA TGAAAGCTGT CAAGCAGAGT 
GTGCTGGACG GCACATCCAA GTGGTCTGCC AAAATTAGCA TCACAGTGGT CTGTGCCCAG 
GGCTTGCAGG CCAAGGACAA GACAGGATCC AGTGACCCTT ATGTCACCGT CCAGGTTGGG 
AAGACCAAGA AAAGGACAAA AACCATCTAC GGGAACCTCA ACCCAGTGTG GGAAGAGAAT 
TTTCATTTTG AATGTCACAA CTCCTCTGAC CGGATCAAGG TTCGTGTTTG GGATGAAGAT 
GACGACATAA AATCCCGTGT GAAACAAAGG TTTAAGAGGG AGTCTGATGA CTTCCTAGGG 
CAGACAATCA TCGAGGTGCG GACGCTTAGC GGCGAGATGG ATGTGTGGTA CAATCTGGAC 
AAGAGAACGG ACAAGTCGGC GGTGTCGGGG GCCATCCGGC TTCACATCAG TGTGGAGATC 
AAAGGGGAGG AGAAGGTGGC GCCCTACCAT GTCCAGTACA CCTGTCTGCA TGAGAACCTG 
TTCCACTTTG TGACGGACGT GCAGAACAAT GGTGTGGTGA AGATTCCCGA TGCCAAGGGT 
GACGACGCCT GGAAGGTTTA CTACGATGAG ACGGCCCAGG AGATCGTGGA TGAGTTTGCC 
ATGCGCTATG GTGTTGAGTC CATCTACCAA GCCATGACCC ACTTTGCCTG CCTCTCCTCC 
AAGTATATGT GCCCTGGGGT ACCCGCTGTC ATGAGCACCC TGCTTGCCAA CATCAACGCC 
TACTACGCAC ACACCACCGC CTCCACCAAC GTGTCTGCCT CTGACCGCTT CGCTGCCTCT 
AATTTCGGGA AAGAGCGCTT TGTGAAACTT CTGGACCAGC TACACAATTC TCTGCGGATC 
GACCTGTCCA TGTACCGGAA CAACTTCCCA GCCAGCAGCC CTGAGCGGCT GCAGGATCTC 
AAGTCCACGG TGGACCTGCT CACCAGCATC ACCTTCTTCC GGATGAAGGT TCAGGAACTG 
CAGAGCCCGC CGCGTGCCAG CCAGGTAGTG AAAGACTGTG TGAAGGCCTG CCTCAACTCC 
ACCTATGAGT ACATCTTCAA CAACTGCCAT GAGCTCTATG GCCGGGAGTA CCAAACGGAT 
CCGGCCAAGA AGGGGGAGGT TCCCCCAGAG GAGCAAGGCC CTAGCATCAA GAACCTGGAT 
TTCTGGTCCA AGCTGATCAC CCTCATCGTG TCTATCATCG AGGAGGATAA GAATTCTTAC 
ACACCCTGCC TCAATCAGTT TCCCCAGGAG CTCAATGTGG GGAAGATCAG TGCTGAGGTG 
ATGTGGAGCC TATTTGCCCA AGACATGAAG TACGCCATGG AGGAACATGA CAAACACCGG 
CTGTGTAAGA GCGCAGATTA CATGAACCTG CACTTCAAGG TGAAGTGGCT GTACAATGAG 
TACGTGGCCG AGCTGCCCAC CTTCAAGGAC CGTGTGCCTG AGTACCCTGC GTGGTTTGAG 
CCCTTCGTCA TCCAGTGGTT GGATGAGAAT GAGGAGGTGT CCCGGGACTT CCTGCATGGT 
GCCCTCGAGC GGGACAAGAA GGACGGGTTC CAGCAGACCT CAGAGCACGC CCTGTTCTCT 
TGCTCAGTGG TGGACGTCTT CTCCCAGCTT AACCAGAGTT TCGAGATCAT CAAGAAGCTG 
GAGTGTCCTG ACCCCCAGAT CGTGGGCCAT TACATGCGGC GTTTTGCCAA GACCATTAGC 
AATGTGCTTC TCCAGTATGC AGACATCGTT TCCAAGGACT TCGCCTCCTA CTGCTCCAAG 
GAGAAGGAGA AAGTGCCCTG CATCCTCATG AACAACACAC AGCAACTACG AGTGCAGCTG 
GAGAAGATGT TTGAGGCGAT GGGCGGGAAG GAGCTGGATG CCGAGGCCAG TGGCACCCTG 
AAGGAGCTGC AGGTGAAACT CAACAATGTC CTGGATGAAC TCAGCCATGT GTTTGCCACC 
AGCTTCCAGC CACACATTGA GGAATGCGTC AGACAGATGG GTGACATCCT GAGCCAGGTG 
AAGGGCACAG GCAATGTACC TGCCAGTGCC TGCAGCAGCG TGGCCCAGGA TGCAGACAAT 
GTGCTGCAGC CCATCATGGA TCTTCTGGAC AGCAACCTCA CCCTGTTTGC CAAAATCTGT 
GAGAAGACAG TTCTGAAGCG GGTGCTGAAG GAGCTCTGGA AGCTGGTGAT GAACACAATG 
GAGAAGACCA TCGTCCTGCC ACCGCTCACT GACCAGACGG GGACACAAAT GATCTTCAAT 
GCTGCCAAGG AGCTGGGCCA GCTGTCCAAA CTTAAGGATC ACATGGTGCG GGAAGAAGCC 
AAGAGCTTGA CCCCGAAGCA GTGTGCTGTT GTTGAACTGG CCCTGGACAC CATCAAGCAA 
TACTTCCACG CGGGGGGCGT TGGCCTCAAG AAGACCTTCC TGGAGAAGAG CCCGGACCTC 
CAGTCCCTGC GCTACGCCCT GTCGCTCTAC ACGCAAGCCA CCGACCTGCT CATCAAAACC 
TTCGTGCAGA CGCAGTCAGC GCAGGGCTCT GGTGTGGAGG ACCCTGTAGG TGAAGTGTCC 
GTCCACGTGG AGCTGTTCAC ACATCCAGGA ACTGGGGAAC AGAAGGTCAC AGTGAAGGTG 
GTGGCCGCCA ATGACCTCAA GTGGCAGACT TCTGGCATCT TCCGCCCGTT CATCGAGGTC 
AACATCGTTG GACCTCAGCT TAGCGACAAG AAACGCAAGT TCGCCACCAA ATCCAAAAAC 
AACAGCTGGG CGCCCAAGTA TAACGAGAGC TTCCAGTTCT CCCTGAGCGC CGACGCGGGC 
CCCGAGTGCT ACGAGCTGCA GGTGTGCGTG AAGGACTACT GCTTTGCGCG CGAAGACCGC 
ACGGTGGGGC TGGCGGTGCT GCAACTGCGA GAGCTGGCCC AGCGCGGGAG CGCCGCGTGC 
TGGCTTCCAC TCGGCCGCCG CATCCACATG GACGACACGG GGCTCACAGT GCTGCGCATC 
CTGTCGCAGC GCAGCAACGA TGAGGTGGCC AAGGAATTCG TCAAGCTCAA GTCGGACACG 
CGCTCAGCCG AGGAGGGCGG TGCCGCGCCT GCGCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Unc13a ( XM_017312882.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168371.1 Mus musculus unc-13 homolog A (C. elegans) (Unc13a), transcript variant X12, mRNA.

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CloneID	OMu82532
Clone ID Related Accession (Same CDS sequence)	XM_017312882.1 , XM_017312882.2
Accession Version	XM_017312882.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein unc-13 homolog A isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTGC TGTGCGTGGG AGTCAAAAAA GCCAAGTTTG ACGGTGCCCA AGAGAAGTTC 
AACACATACG TGACGCTGAA GGTGCAGAAC GTGAAGAGCA CTACCATAGC TGTACGTGGC 
AGCCAGCCCA GCTGGGAGCA GGACTTCATG TTTGAGATCA ACCGCCTGGA TCTAGGCCTG 
ACCGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGTAC TGTGTGGATC 
CCGCTGCGGA CCATCCGCCA GTCCAATGAG GAGGGCCCAG GAGAGTGGCT CACACTGGAC 
TCTCAGGCCA TCATGGCGGA CAGTGAGATC TGTGGGACCA AGGACCCCAC CTTCCATCGC 
ATCCTCCTGG ACGCTCATTT CGAGCTGCCG TTGGACATCC CTGAGGAGGA GGCACGCTAC 
TGGGCCAAGA AGCTGGAGCA GCTGAATGCC ATGCGTGACC AGGATGAGTA CTCCTTCCAG 
GACCAGCAGG ACAAGCCGCT GCCAGTGCCC AGCAGCCAGT GCTGCAACTG GAATTACTTT 
GGCTGGGGAG AACAGAATGA CGACCCCGAC AGTGCGGTGG ATGACCGGGA CAGTGACTAT 
AGGAGTGAGA CCAGCAACAG CATCCCACCG CCCTACTATA CGACTTCGCA GCCCAACGCC 
TCTGTGCACC AGTACTCCGT GCGACCGCCC CCTCTGGGGT CCCGGGAGTC CTACAGTGAC 
TCCATGCACA GCTATGAGGA GTTCTCTGAG CCGCGGGCGC TCAGCCCCAC AGGCAGCAGC 
CGCTATGCTT CTTCCGGGGA GCTGAGCCAG GGAAGCTCAC AGCTGAGCGA GGACTTCGAC 
CCTGATGAGC ACAGCCTGCA GGGCTCGGAG CTGGATGACG AGAGGGACCG CGATTCTTAT 
CACTCCTGTC ACAGCTCCGT GAGTTACCAC AAGGACTCAC CCCGCTGGGA CCAGGACGAT 
GAGGACCTGG AGGACCTGGA GGACCTGGAA GATGAGGAGC TGCCCGAGGA GGAGGAGGAG 
TTGGAGGAGG AGGGGGAGGA GGAATTGGAG GAAGAGGATT TGGAGGAGGA GGAGGAGGTG 
CCTGATGACC TGGCCAGCTA CACCCAGCAG GAGGACACCA CTGTGGCTGA GCCCAAAGAG 
TTCAAGCGGA TCAGCTTCCC AACGGCTGCG CCTCAGAAGG ATGACAAGGT TTCAGCTGTG 
CCTACAGAGG CCCCCGAGGT GGCCAAAGGC ATCCCCAAAG CAGCCACACC CGAGGAGAAG 
GCAGCTGCAG AGCGTGCGCA GGAAGCCGAG CCCCCCAAGT CTGAGGAGAG TTTCAGATCT 
CGAGAGGAGG AAGAGGGCCA GGAAGGGCAG GATGCCATGT CCAGAGCCAA AGCCAACTGG 
TTGCGAGCCT TCAACAAGGT GCGCATGCAG CTGCAGGAGG CCCGAGGAGA AGGGGATATG 
TCTAAGTCTC TATGGTTCAA AGGCGGTCCT GGTGGTGGCC TTATCATCAT TGACAGCATG 
CCAGACATCA GGAAACGGAA GCCCATTCCC CTCGTGAGCG ACCTGGCTAT GTCATTGGTG 
CAGTCTCGGA AGGCAGGCAT CACCTCGGCC TTGGCCTCCA GCACGTTGAA CAATGAAGAG 
CTGAAAAATC ACGTTTACAA GAAGACCCTG CAAGCCTTAA TCTACCCCAT CTCCTGCACC 
ACGCCGCACA ATTTCGAGGT GTGGACGGCC ACCACGCCCA CCTACTGCTA TGAGTGCGAG 
GGGCTGCTGT GGGGCATCGC GCGGCAGGGC ATGCGATGCA CCGAGTGCGG CGTTAAGTGC 
CACGAGAAGT GCCAGGACCT GCTCAATGCG GATTGCCTGC AGCGGGCGGC TGAGAAGAGT 
TCCAAGCATG GCGCTGAAGA CCGCACGCAG AACATCATCA TGGTGCTGAA GGACCGCATG 
AAGATCCGCG AGCGCAACAA GCCTGAGATC TTCGAGCTTA TCCAAGAGAT CTTCGCGGTC 
ACCAAGAGCG CACACACACA GCAGATGAAA GCTGTCAAGC AGAGTGTGCT GGACGGCACA 
TCCAAGTGGT CTGCCAAAAT TAGCATCACA GTGGTCTGTG CCCAGGGCTT GCAGGCCAAG 
GACAAGACAG GATCCAGTGA CCCTTATGTC ACCGTCCAGG TTGGGAAGAC CAAGAAAAGG 
ACAAAAACCA TCTACGGGAA CCTCAACCCA GTGTGGGAAG AGAATTTTCA TTTTGAATGT 
CACAACTCCT CTGACCGGAT CAAGGTTCGT GTTTGGGATG AAGATGACGA CATAAAATCC 
CGTGTGAAAC AAAGGTTTAA GAGGGAGTCT GATGACTTCC TAGGGCAGAC AATCATCGAG 
GTGCGGACGC TTAGCGGCGA GATGGATGTG TGGTACAATC TGGACAAGAG AACGGACAAG 
TCGGCGGTGT CGGGGGCCAT CCGGCTTCAC ATCAGTGTGG AGATCAAAGG GGAGGAGAAG 
GTGGCGCCCT ACCATGTCCA GTACACCTGT CTGCATGAGA ACCTGTTCCA CTTTGTGACG 
GACGTGCAGA ACAATGGTGT GGTGAAGATT CCCGATGCCA AGGGTGACGA CGCCTGGAAG 
GTTTACTACG ATGAGACGGC CCAGGAGATC GTGGATGAGT TTGCCATGCG CTATGGTGTT 
GAGTCCATCT ACCAAGCCAT GACCCACTTT GCCTGCCTCT CCTCCAAGTA TATGTGCCCT 
GGGGTACCCG CTGTCATGAG CACCCTGCTT GCCAACATCA ACGCCTACTA CGCACACACC 
ACCGCCTCCA CCAACGTGTC TGCCTCTGAC CGCTTCGCTG CCTCTAATTT CGGGAAAGAG 
CGCTTTGTGA AACTTCTGGA CCAGCTACAC AATTCTCTGC GGATCGACCT GTCCATGTAC 
CGGAACAACT TCCCAGCCAG CAGCCCTGAG CGGCTGCAGG ATCTCAAGTC CACGGTGGAC 
CTGCTCACCA GCATCACCTT CTTCCGGATG AAGGTTCAGG AACTGCAGAG CCCGCCGCGT 
GCCAGCCAGG TAGTGAAAGA CTGTGTGAAG GCCTGCCTCA ACTCCACCTA TGAGTACATC 
TTCAACAACT GCCATGAGCT CTATGGCCGG GAGTACCAAA CGGATCCGGC CAAGAAGGGG 
GAGGTTCCCC CAGAGGAGCA AGGCCCTAGC ATCAAGAACC TGGATTTCTG GTCCAAGCTG 
ATCACCCTCA TCGTGTCTAT CATCGAGGAG GATAAGAATT CTTACACACC CTGCCTCAAT 
CAGTTTCCCC AGGAGCTCAA TGTGGGGAAG ATCAGTGCTG AGGTGATGTG GAGCCTATTT 
GCCCAAGACA TGAAGTACGC CATGGAGGAA CATGACAAAC ACCGGCTGTG TAAGAGCGCA 
GATTACATGA ACCTGCACTT CAAGGTGAAG TGGCTGTACA ATGAGTACGT GGCCGAGCTG 
CCCACCTTCA AGGACCGTGT GCCTGAGTAC CCTGCGTGGT TTGAGCCCTT CGTCATCCAG 
TGGTTGGATG AGAATGAGGA GGTGTCCCGG GACTTCCTGC ATGGTGCCCT CGAGCGGGAC 
AAGAAGGACG GGTTCCAGCA GACCTCAGAG CACGCCCTGT TCTCTTGCTC AGTGGTGGAC 
GTCTTCTCCC AGCTTAACCA GAGTTTCGAG ATCATCAAGA AGCTGGAGTG TCCTGACCCC 
CAGATCGTGG GCCATTACAT GCGGCGTTTT GCCAAGACCA TTAGCAATGT GCTTCTCCAG 
TATGCAGACA TCGTTTCCAA GGACTTCGCC TCCTACTGCT CCAAGGAGAA GGAGAAAGTG 
CCCTGCATCC TCATGAACAA CACACAGCAA CTACGAGTGC AGCTGGAGAA GATGTTTGAG 
GCGATGGGCG GGAAGGAGCT GGATGCCGAG GCCAGTGGCA CCCTGAAGGA GCTGCAGGTG 
AAACTCAACA ATGTCCTGGA TGAACTCAGC CATGTGTTTG CCACCAGCTT CCAGCCACAC 
ATTGAGGAAT GCGTCAGACA GATGGGTGAC ATCCTGAGCC AGGTGAAGGG CACAGGCAAT 
GTACCTGCCA GTGCCTGCAG CAGCGTGGCC CAGGATGCAG ACAATGTGCT GCAGCCCATC 
ATGGATCTTC TGGACAGCAA CCTCACCCTG TTTGCCAAAA TCTGTGAGAA GACAGTTCTG 
AAGCGGGTGC TGAAGGAGCT CTGGAAGCTG GTGATGAACA CAATGGAGAA GACCATCGTC 
CTGCCACCGC TCACTGACCA GACGGGGACA CAAATGATCT TCAATGCTGC CAAGGAGCTG 
GGCCAGCTGT CCAAACTTAA GGATCACATG GTGCGGGAAG AAGCCAAGAG CTTGACCCCG 
AAGCAGTGTG CTGTTGTTGA ACTGGCCCTG GACACCATCA AGCAATACTT CCACGCGGGG 
GGCGTTGGCC TCAAGAAGAC CTTCCTGGAG AAGAGCCCGG ACCTCCAGTC CCTGCGCTAC 
GCCCTGTCGC TCTACACGCA AGCCACCGAC CTGCTCATCA AAACCTTCGT GCAGACGCAG 
TCAGCGCAGG TCCATGGTGG AAAAGGGACT AGGTTTACCC TTAGTGAAGA CGTTTGTCCT 
GAGATGGGCT CTGGTGTGGA GGACCCTGTA GGTGAAGTGT CCGTCCACGT GGAGCTGTTC 
ACACATCCAG GAACTGGGGA ACAGAAGGTC ACAGTGAAGG TGGTGGCCGC CAATGACCTC 
AAGTGGCAGA CTTCTGGCAT CTTCCGCCCG TTCATCGAGG TCAACATCGT TGGACCTCAG 
CTTAGCGACA AGAAACGCAA GTTCGCCACC AAATCCAAAA ACAACAGCTG GGCGCCCAAG 
TATAACGAGA GCTTCCAGTT CTCCCTGAGC GCCGACGCGG GCCCCGAGTG CTACGAGCTG 
CAGGTGTGCG TGAAGGACTA CTGCTTTGCG CGCGAAGACC GCACGGTGGG GCTGGCGGTG 
CTGCAACTGC GAGAGCTGGC CCAGCGCGGG AGCGCCGCGT GCTGGCTTCC ACTCGGCCGC 
CGCATCCACA TGGACGACAC GGGGCTCACA GTGCTGCGCA TCCTGTCGCA GCGCAGCAAC 
GATGAGGTGG CCAAGGAATT CGTCAAGCTC AAGTCGGACA CGCGCTCAGC CGAGGAGGGC 
GGTGCCGCGC CTGCGCCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168371.1
CDS	125..5245
Translation	MSLLCVGVKKAKFDGAQEKFNTYVTLKVQNVKSTTIAVRGSQPSWEQDFMFEINRLDLGLTVEVWNKGLIWDTMVGTVWIPLRTIRQSNEEGPGEWLTLDSQAIMADSEICGTKDPTFHRILLDAHFELPLDIPEEEARYWAKKLEQLNAMRDQDEYSFQDQQDKPLPVPSSQCCNWNYFGWGEQNDDPDSAVDDRDSDYRSETSNSIPPPYYTTSQPNASVHQYSVRPPPLGSRESYSDSMHSYEEFSEPRALSPTGSSRYASSGELSQGSSQLSEDFDPDEHSLQGSELDDERDRDSYHSCHSSVSYHKDSPRWDQDDEDLEDLEDLEDEELPEEEEELEEEGEEELEEEDLEEEEEVPDDLASYTQQEDTTVAEPKEFKRISFPTAAPQKDDKVSAVPTEAPEVAKGIPKAATPEEKAAAERAQEAEPPKSEESFRSREEEEGQEGQDAMSRAKANWLRAFNKVRMQLQEARGEGDMSKSLWFKGGPGGGLIIIDSMPDIRKRKPIPLVSDLAMSLVQSRKAGITSALASSTLNNEELKNHVYKKTLQALIYPISCTTPHNFEVWTATTPTYCYECEGLLWGIARQGMRCTECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMVLKDRMKIRERNKPEIFELIQEIFAVTKSAHTQQMKAVKQSVLDGTSKWSAKISITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIYGNLNPVWEENFHFECHNSSDRIKVRVWDEDDDIKSRVKQRFKRESDDFLGQTIIEVRTLSGEMDVWYNLDKRTDKSAVSGAIRLHISVEIKGEEKVAPYHVQYTCLHENLFHFVTDVQNNGVVKIPDAKGDDAWKVYYDETAQEIVDEFAMRYGVESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSMYRNNFPASSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHELYGREYQTDPAKKGEVPPEEQGPSIKNLDFWSKLITLIVSIIEEDKNSYTPCLNQFPQELNVGKISAEVMWSLFAQDMKYAMEEHDKHRLCKSADYMNLHFKVKWLYNEYVAELPTFKDRVPEYPAWFEPFVIQWLDENEEVSRDFLHGALERDKKDGFQQTSEHALFSCSVVDVFSQLNQSFEIIKKLECPDPQIVGHYMRRFAKTISNVLLQYADIVSKDFASYCSKEKEKVPCILMNNTQQLRVQLEKMFEAMGGKELDAEASGTLKELQVKLNNVLDELSHVFATSFQPHIEECVRQMGDILSQVKGTGNVPASACSSVAQDADNVLQPIMDLLDSNLTLFAKICEKTVLKRVLKELWKLVMNTMEKTIVLPPLTDQTGTQMIFNAAKELGQLSKLKDHMVREEAKSLTPKQCAVVELALDTIKQYFHAGGVGLKKTFLEKSPDLQSLRYALSLYTQATDLLIKTFVQTQSAQVHGGKGTRFTLSEDVCPEMGSGVEDPVGEVSVHVELFTHPGTGEQKVTVKVVAANDLKWQTSGIFRPFIEVNIVGPQLSDKKRKFATKSKNNSWAPKYNESFQFSLSADAGPECYELQVCVKDYCFAREDRTVGLAVLQLRELAQRGSAACWLPLGRRIHMDDTGLTVLRILSQRSNDEVAKEFVKLKSDTRSAEEGGAAPAP

Target ORF information:

RefSeq Version	XM_017312882.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus unc-13 homolog A (C. elegans) (Unc13a), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312882.1

ORF Insert Sequence:

ATGTCTCTGC TGTGCGTGGG AGTCAAAAAA GCCAAGTTTG ACGGTGCCCA AGAGAAGTTC 
AACACATACG TGACGCTGAA GGTGCAGAAC GTGAAGAGCA CTACCATAGC TGTACGTGGC 
AGCCAGCCCA GCTGGGAGCA GGACTTCATG TTTGAGATCA ACCGCCTGGA TCTAGGCCTG 
ACCGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGTAC TGTGTGGATC 
CCGCTGCGGA CCATCCGCCA GTCCAATGAG GAGGGCCCAG GAGAGTGGCT CACACTGGAC 
TCTCAGGCCA TCATGGCGGA CAGTGAGATC TGTGGGACCA AGGACCCCAC CTTCCATCGC 
ATCCTCCTGG ACGCTCATTT CGAGCTGCCG TTGGACATCC CTGAGGAGGA GGCACGCTAC 
TGGGCCAAGA AGCTGGAGCA GCTGAATGCC ATGCGTGACC AGGATGAGTA CTCCTTCCAG 
GACCAGCAGG ACAAGCCGCT GCCAGTGCCC AGCAGCCAGT GCTGCAACTG GAATTACTTT 
GGCTGGGGAG AACAGAATGA CGACCCCGAC AGTGCGGTGG ATGACCGGGA CAGTGACTAT 
AGGAGTGAGA CCAGCAACAG CATCCCACCG CCCTACTATA CGACTTCGCA GCCCAACGCC 
TCTGTGCACC AGTACTCCGT GCGACCGCCC CCTCTGGGGT CCCGGGAGTC CTACAGTGAC 
TCCATGCACA GCTATGAGGA GTTCTCTGAG CCGCGGGCGC TCAGCCCCAC AGGCAGCAGC 
CGCTATGCTT CTTCCGGGGA GCTGAGCCAG GGAAGCTCAC AGCTGAGCGA GGACTTCGAC 
CCTGATGAGC ACAGCCTGCA GGGCTCGGAG CTGGATGACG AGAGGGACCG CGATTCTTAT 
CACTCCTGTC ACAGCTCCGT GAGTTACCAC AAGGACTCAC CCCGCTGGGA CCAGGACGAT 
GAGGACCTGG AGGACCTGGA GGACCTGGAA GATGAGGAGC TGCCCGAGGA GGAGGAGGAG 
TTGGAGGAGG AGGGGGAGGA GGAATTGGAG GAAGAGGATT TGGAGGAGGA GGAGGAGGTG 
CCTGATGACC TGGCCAGCTA CACCCAGCAG GAGGACACCA CTGTGGCTGA GCCCAAAGAG 
TTCAAGCGGA TCAGCTTCCC AACGGCTGCG CCTCAGAAGG ATGACAAGGT TTCAGCTGTG 
CCTACAGAGG CCCCCGAGGT GGCCAAAGGC ATCCCCAAAG CAGCCACACC CGAGGAGAAG 
GCAGCTGCAG AGCGTGCGCA GGAAGCCGAG CCCCCCAAGT CTGAGGAGAG TTTCAGATCT 
CGAGAGGAGG AAGAGGGCCA GGAAGGGCAG GATGCCATGT CCAGAGCCAA AGCCAACTGG 
TTGCGAGCCT TCAACAAGGT GCGCATGCAG CTGCAGGAGG CCCGAGGAGA AGGGGATATG 
TCTAAGTCTC TATGGTTCAA AGGCGGTCCT GGTGGTGGCC TTATCATCAT TGACAGCATG 
CCAGACATCA GGAAACGGAA GCCCATTCCC CTCGTGAGCG ACCTGGCTAT GTCATTGGTG 
CAGTCTCGGA AGGCAGGCAT CACCTCGGCC TTGGCCTCCA GCACGTTGAA CAATGAAGAG 
CTGAAAAATC ACGTTTACAA GAAGACCCTG CAAGCCTTAA TCTACCCCAT CTCCTGCACC 
ACGCCGCACA ATTTCGAGGT GTGGACGGCC ACCACGCCCA CCTACTGCTA TGAGTGCGAG 
GGGCTGCTGT GGGGCATCGC GCGGCAGGGC ATGCGATGCA CCGAGTGCGG CGTTAAGTGC 
CACGAGAAGT GCCAGGACCT GCTCAATGCG GATTGCCTGC AGCGGGCGGC TGAGAAGAGT 
TCCAAGCATG GCGCTGAAGA CCGCACGCAG AACATCATCA TGGTGCTGAA GGACCGCATG 
AAGATCCGCG AGCGCAACAA GCCTGAGATC TTCGAGCTTA TCCAAGAGAT CTTCGCGGTC 
ACCAAGAGCG CACACACACA GCAGATGAAA GCTGTCAAGC AGAGTGTGCT GGACGGCACA 
TCCAAGTGGT CTGCCAAAAT TAGCATCACA GTGGTCTGTG CCCAGGGCTT GCAGGCCAAG 
GACAAGACAG GATCCAGTGA CCCTTATGTC ACCGTCCAGG TTGGGAAGAC CAAGAAAAGG 
ACAAAAACCA TCTACGGGAA CCTCAACCCA GTGTGGGAAG AGAATTTTCA TTTTGAATGT 
CACAACTCCT CTGACCGGAT CAAGGTTCGT GTTTGGGATG AAGATGACGA CATAAAATCC 
CGTGTGAAAC AAAGGTTTAA GAGGGAGTCT GATGACTTCC TAGGGCAGAC AATCATCGAG 
GTGCGGACGC TTAGCGGCGA GATGGATGTG TGGTACAATC TGGACAAGAG AACGGACAAG 
TCGGCGGTGT CGGGGGCCAT CCGGCTTCAC ATCAGTGTGG AGATCAAAGG GGAGGAGAAG 
GTGGCGCCCT ACCATGTCCA GTACACCTGT CTGCATGAGA ACCTGTTCCA CTTTGTGACG 
GACGTGCAGA ACAATGGTGT GGTGAAGATT CCCGATGCCA AGGGTGACGA CGCCTGGAAG 
GTTTACTACG ATGAGACGGC CCAGGAGATC GTGGATGAGT TTGCCATGCG CTATGGTGTT 
GAGTCCATCT ACCAAGCCAT GACCCACTTT GCCTGCCTCT CCTCCAAGTA TATGTGCCCT 
GGGGTACCCG CTGTCATGAG CACCCTGCTT GCCAACATCA ACGCCTACTA CGCACACACC 
ACCGCCTCCA CCAACGTGTC TGCCTCTGAC CGCTTCGCTG CCTCTAATTT CGGGAAAGAG 
CGCTTTGTGA AACTTCTGGA CCAGCTACAC AATTCTCTGC GGATCGACCT GTCCATGTAC 
CGGAACAACT TCCCAGCCAG CAGCCCTGAG CGGCTGCAGG ATCTCAAGTC CACGGTGGAC 
CTGCTCACCA GCATCACCTT CTTCCGGATG AAGGTTCAGG AACTGCAGAG CCCGCCGCGT 
GCCAGCCAGG TAGTGAAAGA CTGTGTGAAG GCCTGCCTCA ACTCCACCTA TGAGTACATC 
TTCAACAACT GCCATGAGCT CTATGGCCGG GAGTACCAAA CGGATCCGGC CAAGAAGGGG 
GAGGTTCCCC CAGAGGAGCA AGGCCCTAGC ATCAAGAACC TGGATTTCTG GTCCAAGCTG 
ATCACCCTCA TCGTGTCTAT CATCGAGGAG GATAAGAATT CTTACACACC CTGCCTCAAT 
CAGTTTCCCC AGGAGCTCAA TGTGGGGAAG ATCAGTGCTG AGGTGATGTG GAGCCTATTT 
GCCCAAGACA TGAAGTACGC CATGGAGGAA CATGACAAAC ACCGGCTGTG TAAGAGCGCA 
GATTACATGA ACCTGCACTT CAAGGTGAAG TGGCTGTACA ATGAGTACGT GGCCGAGCTG 
CCCACCTTCA AGGACCGTGT GCCTGAGTAC CCTGCGTGGT TTGAGCCCTT CGTCATCCAG 
TGGTTGGATG AGAATGAGGA GGTGTCCCGG GACTTCCTGC ATGGTGCCCT CGAGCGGGAC 
AAGAAGGACG GGTTCCAGCA GACCTCAGAG CACGCCCTGT TCTCTTGCTC AGTGGTGGAC 
GTCTTCTCCC AGCTTAACCA GAGTTTCGAG ATCATCAAGA AGCTGGAGTG TCCTGACCCC 
CAGATCGTGG GCCATTACAT GCGGCGTTTT GCCAAGACCA TTAGCAATGT GCTTCTCCAG 
TATGCAGACA TCGTTTCCAA GGACTTCGCC TCCTACTGCT CCAAGGAGAA GGAGAAAGTG 
CCCTGCATCC TCATGAACAA CACACAGCAA CTACGAGTGC AGCTGGAGAA GATGTTTGAG 
GCGATGGGCG GGAAGGAGCT GGATGCCGAG GCCAGTGGCA CCCTGAAGGA GCTGCAGGTG 
AAACTCAACA ATGTCCTGGA TGAACTCAGC CATGTGTTTG CCACCAGCTT CCAGCCACAC 
ATTGAGGAAT GCGTCAGACA GATGGGTGAC ATCCTGAGCC AGGTGAAGGG CACAGGCAAT 
GTACCTGCCA GTGCCTGCAG CAGCGTGGCC CAGGATGCAG ACAATGTGCT GCAGCCCATC 
ATGGATCTTC TGGACAGCAA CCTCACCCTG TTTGCCAAAA TCTGTGAGAA GACAGTTCTG 
AAGCGGGTGC TGAAGGAGCT CTGGAAGCTG GTGATGAACA CAATGGAGAA GACCATCGTC 
CTGCCACCGC TCACTGACCA GACGGGGACA CAAATGATCT TCAATGCTGC CAAGGAGCTG 
GGCCAGCTGT CCAAACTTAA GGATCACATG GTGCGGGAAG AAGCCAAGAG CTTGACCCCG 
AAGCAGTGTG CTGTTGTTGA ACTGGCCCTG GACACCATCA AGCAATACTT CCACGCGGGG 
GGCGTTGGCC TCAAGAAGAC CTTCCTGGAG AAGAGCCCGG ACCTCCAGTC CCTGCGCTAC 
GCCCTGTCGC TCTACACGCA AGCCACCGAC CTGCTCATCA AAACCTTCGT GCAGACGCAG 
TCAGCGCAGG TCCATGGTGG AAAAGGGACT AGGTTTACCC TTAGTGAAGA CGTTTGTCCT 
GAGATGGGCT CTGGTGTGGA GGACCCTGTA GGTGAAGTGT CCGTCCACGT GGAGCTGTTC 
ACACATCCAG GAACTGGGGA ACAGAAGGTC ACAGTGAAGG TGGTGGCCGC CAATGACCTC 
AAGTGGCAGA CTTCTGGCAT CTTCCGCCCG TTCATCGAGG TCAACATCGT TGGACCTCAG 
CTTAGCGACA AGAAACGCAA GTTCGCCACC AAATCCAAAA ACAACAGCTG GGCGCCCAAG 
TATAACGAGA GCTTCCAGTT CTCCCTGAGC GCCGACGCGG GCCCCGAGTG CTACGAGCTG 
CAGGTGTGCG TGAAGGACTA CTGCTTTGCG CGCGAAGACC GCACGGTGGG GCTGGCGGTG 
CTGCAACTGC GAGAGCTGGC CCAGCGCGGG AGCGCCGCGT GCTGGCTTCC ACTCGGCCGC 
CGCATCCACA TGGACGACAC GGGGCTCACA GTGCTGCGCA TCCTGTCGCA GCGCAGCAAC 
GATGAGGTGG CCAAGGAATT CGTCAAGCTC AAGTCGGACA CGCGCTCAGC CGAGGAGGGC 
GGTGCCGCGC CTGCGCCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Unc13a ( XM_017312883.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168372.1 Mus musculus unc-13 homolog A (C. elegans) (Unc13a), transcript variant X13, mRNA.

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CloneID	OMu82533
Clone ID Related Accession (Same CDS sequence)	XM_017312883.2 , XM_017312883.1
Accession Version	XM_017312883.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5064bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein unc-13 homolog A isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTGC TGTGCGTGGG AGTCAAAAAA GCCAAGTTTG ACGGTGCCCA AGAGAAGTTC 
AACACATACG TGACGCTGAA GGTGCAGAAC GTGAAGAGCA CTACCATAGC TGTACGTGGC 
AGCCAGCCCA GCTGGGAGCA GGACTTCATG TTTGAGATCA ACCGCCTGGA TCTAGGCCTG 
ACCGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGTAC TGTGTGGATC 
CCGCTGCGGA CCATCCGCCA GTCCAATGAG GAGGGCCCAG GAGAGTGGCT CACACTGGAC 
TCTCAGGCCA TCATGGCGGA CAGTGAGATC TGTGGGACCA AGGACCCCAC CTTCCATCGC 
ATCCTCCTGG ACGCTCATTT CGAGCTGCCG TTGGACATCC CTGAGGAGGA GGCACGCTAC 
TGGGCCAAGA AGCTGGAGCA GCTGAATGCC ATGCGTGACC AGGATGAGTA CTCCTTCCAG 
GACCAGCAGG ACAAGCCGCT GCCAGTGCCC AGCAGCCAGT GCTGCAACTG GAATTACTTT 
GGCTGGGGAG AACAGAATGA CGACCCCGAC AGTGCGGTGG ATGACCGGGA CAGTGACTAT 
AGGAGTGAGA CCAGCAACAG CATCCCACCG CCCTACTATA CGACTTCGCA GCCCAACGCC 
TCTGTGCACC AGTACTCCGT GCGACCGCCC CCTCTGGGGT CCCGGGAGTC CTACAGTGAC 
TCCATGCACA GCTATGAGGA GTTCTCTGAG CCGCGGGCGC TCAGCCCCAC AGGCAGCAGC 
CGCTATGCTT CTTCCGGGGA GCTGAGCCAG GGAAGCTCAC AGCTGAGCGA GGACTTCGAC 
CCTGATGAGC ACAGCCTGCA GGGCTCGGAG CTGGATGACG AGAGGGACCG CGATTCTTAT 
CACTCCTGTC ACAGCTCCGT GAGTTACCAC AAGGACTCAC CCCGCTGGGA CCAGGACGAT 
GAGGACCTGG AGGACCTGGA GGACCTGGAA GATGAGGAGC TGCCCGAGGA GGAGGAGGAG 
TTGGAGGAGG AGGGGGAGGA GGAATTGGAG GAAGAGGATT TGGAGGAGGA GGAGGAGGTG 
CCTGATGACC TGGCCAGCTA CACCCAGCAG GAGGACACCA CTGTGGCTGA GCCCAAAGAG 
TTCAAGCGGA TCAGCTTCCC AACGGCTGCG CCTCAGAAGG ATGACAAGGT TTCAGCTGTG 
CCTACAGAGG CCCCCGAGGT GGCCAAAGGC ATCCCCAAAG CAGCCACACC CGAGGAGAAG 
GCAGCTGCAG AGCGTGCGCA GGAAGCCGAG CCCCCCAAGT CTGAGGAGAG TTTCAGATCT 
CGAGAGGAGG AAGAGGGCCA GGAAGGGCAG GATGCCATGT CCAGAGCCAA AGCCAACTGG 
TTGCGAGCCT TCAACAAGGT GCGCATGCAG CTGCAGGAGG CCCGAGGAGA AGGGGATATG 
TCTAAGTCTC TATGGTTCAA AGGCGGTCCT GGTGGTGGCC TTATCATCAT TGACAGCATG 
CCAGACATCA GGAAACGGAA GCCCATTCCC CTCGTGAGCG ACCTGGCTAT GTCATTGGTG 
CAGTCTCGGA AGGCAGGCAT CACCTCGGCC TTGGCCTCCA GCACGTTGAA CAATGAAGAG 
CTGAAAAATC ACGTTTACAA GAAGACCCTG CAAGCCTTAA TCTACCCCAT CTCCTGCACC 
ACGCCGCACA ATTTCGAGGT GTGGACGGCC ACCACGCCCA CCTACTGCTA TGAGTGCGAG 
GGGCTGCTGT GGGGCATCGC GCGGCAGGGC ATGCGATGCA CCGAGTGCGG CGTTAAGTGC 
CACGAGAAGT GCCAGGACCT GCTCAATGCG GATTGCCTGC AGCGGGCGGC TGAGAAGAGT 
TCCAAGCATG GCGCTGAAGA CCGCACGCAG AACATCATCA TGGTGCTGAA GGACCGCATG 
AAGATCCGCG AGCGCAACAA GCCTGAGATC TTCGAGCTTA TCCAAGAGAT CTTCGCGGTC 
ACCAAGAGCG CACACACACA GCAGATGAAA GCTGTCAAGC AGAGTGTGCT GGACGGCACA 
TCCAAGTGGT CTGCCAAAAT TAGCATCACA GTGGTCTGTG CCCAGGGCTT GCAGGCCAAG 
GACAAGACAG GATCCAGTGA CCCTTATGTC ACCGTCCAGG TTGGGAAGAC CAAGAAAAGG 
ACAAAAACCA TCTACGGGAA CCTCAACCCA GTGTGGGAAG AGAATTTTCA TTTTGAATGT 
CACAACTCCT CTGACCGGAT CAAGGTTCGT GTTTGGGATG AAGATGACGA CATAAAATCC 
CGTGTGAAAC AAAGGTTTAA GAGGGAGTCT GATGACTTCC TAGGGCAGAC AATCATCGAG 
GTGCGGACGC TTAGCGGCGA GATGGATGTG TGGTACAATC TGGACAAGAG AACGGACAAG 
TCGGCGGTGT CGGGGGCCAT CCGGCTTCAC ATCAGTGTGG AGATCAAAGG GGAGGAGAAG 
GTGGCGCCCT ACCATGTCCA GTACACCTGT CTGCATGAGA ACCTGTTCCA CTTTGTGACG 
GACGTGCAGA ACAATGGTGT GGTGAAGATT CCCGATGCCA AGGGTGACGA CGCCTGGAAG 
GTTTACTACG ATGAGACGGC CCAGGAGATC GTGGATGAGT TTGCCATGCG CTATGGTGTT 
GAGTCCATCT ACCAAGCCAT GACCCACTTT GCCTGCCTCT CCTCCAAGTA TATGTGCCCT 
GGGGTACCCG CTGTCATGAG CACCCTGCTT GCCAACATCA ACGCCTACTA CGCACACACC 
ACCGCCTCCA CCAACGTGTC TGCCTCTGAC CGCTTCGCTG CCTCTAATTT CGGGAAAGAG 
CGCTTTGTGA AACTTCTGGA CCAGCTACAC AATTCTCTGC GGATCGACCT GTCCATGTAC 
CGGAACAACT TCCCAGCCAG CAGCCCTGAG CGGCTGCAGG ATCTCAAGTC CACGGTGGAC 
CTGCTCACCA GCATCACCTT CTTCCGGATG AAGGTTCAGG AACTGCAGAG CCCGCCGCGT 
GCCAGCCAGG TAGTGAAAGA CTGTGTGAAG GCCTGCCTCA ACTCCACCTA TGAGTACATC 
TTCAACAACT GCCATGAGCT CTATGGCCGG GAGTACCAAA CGGATCCGGC CAAGAAGGGG 
GAGGTTCCCC CAGAGGAGCA AGGCCCTAGC ATCAAGAACC TGGATTTCTG GTCCAAGCTG 
ATCACCCTCA TCGTGTCTAT CATCGAGGAG GATAAGAATT CTTACACACC CTGCCTCAAT 
CAGTTTCCCC AGGAGCTCAA TGTGGGGAAG ATCAGTGCTG AGGTGATGTG GAGCCTATTT 
GCCCAAGACA TGAAGTACGC CATGGAGGAA CATGACAAAC ACCGGCTGTG TAAGAGCGCA 
GATTACATGA ACCTGCACTT CAAGGTGAAG TGGCTGTACA ATGAGTACGT GGCCGAGCTG 
CCCACCTTCA AGGACCGTGT GCCTGAGTAC CCTGCGTGGT TTGAGCCCTT CGTCATCCAG 
TGGTTGGATG AGAATGAGGA GGTGTCCCGG GACTTCCTGC ATGGTGCCCT CGAGCGGGAC 
AAGAAGGACG GGTTCCAGCA GACCTCAGAG CACGCCCTGT TCTCTTGCTC AGTGGTGGAC 
GTCTTCTCCC AGCTTAACCA GAGTTTCGAG ATCATCAAGA AGCTGGAGTG TCCTGACCCC 
CAGATCGTGG GCCATTACAT GCGGCGTTTT GCCAAGACCA TTAGCAATGT GCTTCTCCAG 
TATGCAGACA TCGTTTCCAA GGACTTCGCC TCCTACTGCT CCAAGGAGAA GGAGAAAGTG 
CCCTGCATCC TCATGAACAA CACACAGCAA CTACGAGTGC AGCTGGAGAA GATGTTTGAG 
GCGATGGGCG GGAAGGAGCT GGATGCCGAG GCCAGTGGCA CCCTGAAGGA GCTGCAGGTG 
AAACTCAACA ATGTCCTGGA TGAACTCAGC CATGTGTTTG CCACCAGCTT CCAGCCACAC 
ATTGAGGAAT GCGTCAGACA GATGGGTGAC ATCCTGAGCC AGGTGAAGGG CACAGGCAAT 
GTACCTGCCA GTGCCTGCAG CAGCGTGGCC CAGGATGCAG ACAATGTGCT GCAGCCCATC 
ATGGATCTTC TGGACAGCAA CCTCACCCTG TTTGCCAAAA TCTGTGAGAA GACAGTTCTG 
AAGCGGGTGC TGAAGGAGCT CTGGAAGCTG GTGATGAACA CAATGGAGAA GACCATCGTC 
CTGCCACCGC TCACTGACCA GACGGGGACA CAAATGATCT TCAATGCTGC CAAGGAGCTG 
GGCCAGCTGT CCAAACTTAA GGATCACATG GTGCGGGAAG AAGCCAAGAG CTTGACCCCG 
AAGCAGTGTG CTGTTGTTGA ACTGGCCCTG GACACCATCA AGCAATACTT CCACGCGGGG 
GGCGTTGGCC TCAAGAAGAC CTTCCTGGAG AAGAGCCCGG ACCTCCAGTC CCTGCGCTAC 
GCCCTGTCGC TCTACACGCA AGCCACCGAC CTGCTCATCA AAACCTTCGT GCAGACGCAG 
TCAGCGCAGG GCTCTGGTGT GGAGGACCCT GTAGGTGAAG TGTCCGTCCA CGTGGAGCTG 
TTCACACATC CAGGAACTGG GGAACAGAAG GTCACAGTGA AGGTGGTGGC CGCCAATGAC 
CTCAAGTGGC AGACTTCTGG CATCTTCCGC CCGTTCATCG AGGTCAACAT CGTTGGACCT 
CAGCTTAGCG ACAAGAAACG CAAGTTCGCC ACCAAATCCA AAAACAACAG CTGGGCGCCC 
AAGTATAACG AGAGCTTCCA GTTCTCCCTG AGCGCCGACG CGGGCCCCGA GTGCTACGAG 
CTGCAGGTGT GCGTGAAGGA CTACTGCTTT GCGCGCGAAG ACCGCACGGT GGGGCTGGCG 
GTGCTGCAAC TGCGAGAGCT GGCCCAGCGC GGGAGCGCCG CGTGCTGGCT TCCACTCGGC 
CGCCGCATCC ACATGGACGA CACGGGGCTC ACAGTGCTGC GCATCCTGTC GCAGCGCAGC 
AACGATGAGG TGGCCAAGGA ATTCGTCAAG CTCAAGTCGG ACACGCGCTC AGCCGAGGAG 
GGCGGTGCCG CGCCTGCGCC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168372.1
CDS	125..5188
Translation	MSLLCVGVKKAKFDGAQEKFNTYVTLKVQNVKSTTIAVRGSQPSWEQDFMFEINRLDLGLTVEVWNKGLIWDTMVGTVWIPLRTIRQSNEEGPGEWLTLDSQAIMADSEICGTKDPTFHRILLDAHFELPLDIPEEEARYWAKKLEQLNAMRDQDEYSFQDQQDKPLPVPSSQCCNWNYFGWGEQNDDPDSAVDDRDSDYRSETSNSIPPPYYTTSQPNASVHQYSVRPPPLGSRESYSDSMHSYEEFSEPRALSPTGSSRYASSGELSQGSSQLSEDFDPDEHSLQGSELDDERDRDSYHSCHSSVSYHKDSPRWDQDDEDLEDLEDLEDEELPEEEEELEEEGEEELEEEDLEEEEEVPDDLASYTQQEDTTVAEPKEFKRISFPTAAPQKDDKVSAVPTEAPEVAKGIPKAATPEEKAAAERAQEAEPPKSEESFRSREEEEGQEGQDAMSRAKANWLRAFNKVRMQLQEARGEGDMSKSLWFKGGPGGGLIIIDSMPDIRKRKPIPLVSDLAMSLVQSRKAGITSALASSTLNNEELKNHVYKKTLQALIYPISCTTPHNFEVWTATTPTYCYECEGLLWGIARQGMRCTECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMVLKDRMKIRERNKPEIFELIQEIFAVTKSAHTQQMKAVKQSVLDGTSKWSAKISITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIYGNLNPVWEENFHFECHNSSDRIKVRVWDEDDDIKSRVKQRFKRESDDFLGQTIIEVRTLSGEMDVWYNLDKRTDKSAVSGAIRLHISVEIKGEEKVAPYHVQYTCLHENLFHFVTDVQNNGVVKIPDAKGDDAWKVYYDETAQEIVDEFAMRYGVESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSMYRNNFPASSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHELYGREYQTDPAKKGEVPPEEQGPSIKNLDFWSKLITLIVSIIEEDKNSYTPCLNQFPQELNVGKISAEVMWSLFAQDMKYAMEEHDKHRLCKSADYMNLHFKVKWLYNEYVAELPTFKDRVPEYPAWFEPFVIQWLDENEEVSRDFLHGALERDKKDGFQQTSEHALFSCSVVDVFSQLNQSFEIIKKLECPDPQIVGHYMRRFAKTISNVLLQYADIVSKDFASYCSKEKEKVPCILMNNTQQLRVQLEKMFEAMGGKELDAEASGTLKELQVKLNNVLDELSHVFATSFQPHIEECVRQMGDILSQVKGTGNVPASACSSVAQDADNVLQPIMDLLDSNLTLFAKICEKTVLKRVLKELWKLVMNTMEKTIVLPPLTDQTGTQMIFNAAKELGQLSKLKDHMVREEAKSLTPKQCAVVELALDTIKQYFHAGGVGLKKTFLEKSPDLQSLRYALSLYTQATDLLIKTFVQTQSAQGSGVEDPVGEVSVHVELFTHPGTGEQKVTVKVVAANDLKWQTSGIFRPFIEVNIVGPQLSDKKRKFATKSKNNSWAPKYNESFQFSLSADAGPECYELQVCVKDYCFAREDRTVGLAVLQLRELAQRGSAACWLPLGRRIHMDDTGLTVLRILSQRSNDEVAKEFVKLKSDTRSAEEGGAAPAP

Target ORF information:

RefSeq Version	XM_017312883.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus unc-13 homolog A (C. elegans) (Unc13a), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312883.1

ORF Insert Sequence:

ATGTCTCTGC TGTGCGTGGG AGTCAAAAAA GCCAAGTTTG ACGGTGCCCA AGAGAAGTTC 
AACACATACG TGACGCTGAA GGTGCAGAAC GTGAAGAGCA CTACCATAGC TGTACGTGGC 
AGCCAGCCCA GCTGGGAGCA GGACTTCATG TTTGAGATCA ACCGCCTGGA TCTAGGCCTG 
ACCGTGGAGG TGTGGAACAA GGGTCTCATC TGGGACACGA TGGTGGGTAC TGTGTGGATC 
CCGCTGCGGA CCATCCGCCA GTCCAATGAG GAGGGCCCAG GAGAGTGGCT CACACTGGAC 
TCTCAGGCCA TCATGGCGGA CAGTGAGATC TGTGGGACCA AGGACCCCAC CTTCCATCGC 
ATCCTCCTGG ACGCTCATTT CGAGCTGCCG TTGGACATCC CTGAGGAGGA GGCACGCTAC 
TGGGCCAAGA AGCTGGAGCA GCTGAATGCC ATGCGTGACC AGGATGAGTA CTCCTTCCAG 
GACCAGCAGG ACAAGCCGCT GCCAGTGCCC AGCAGCCAGT GCTGCAACTG GAATTACTTT 
GGCTGGGGAG AACAGAATGA CGACCCCGAC AGTGCGGTGG ATGACCGGGA CAGTGACTAT 
AGGAGTGAGA CCAGCAACAG CATCCCACCG CCCTACTATA CGACTTCGCA GCCCAACGCC 
TCTGTGCACC AGTACTCCGT GCGACCGCCC CCTCTGGGGT CCCGGGAGTC CTACAGTGAC 
TCCATGCACA GCTATGAGGA GTTCTCTGAG CCGCGGGCGC TCAGCCCCAC AGGCAGCAGC 
CGCTATGCTT CTTCCGGGGA GCTGAGCCAG GGAAGCTCAC AGCTGAGCGA GGACTTCGAC 
CCTGATGAGC ACAGCCTGCA GGGCTCGGAG CTGGATGACG AGAGGGACCG CGATTCTTAT 
CACTCCTGTC ACAGCTCCGT GAGTTACCAC AAGGACTCAC CCCGCTGGGA CCAGGACGAT 
GAGGACCTGG AGGACCTGGA GGACCTGGAA GATGAGGAGC TGCCCGAGGA GGAGGAGGAG 
TTGGAGGAGG AGGGGGAGGA GGAATTGGAG GAAGAGGATT TGGAGGAGGA GGAGGAGGTG 
CCTGATGACC TGGCCAGCTA CACCCAGCAG GAGGACACCA CTGTGGCTGA GCCCAAAGAG 
TTCAAGCGGA TCAGCTTCCC AACGGCTGCG CCTCAGAAGG ATGACAAGGT TTCAGCTGTG 
CCTACAGAGG CCCCCGAGGT GGCCAAAGGC ATCCCCAAAG CAGCCACACC CGAGGAGAAG 
GCAGCTGCAG AGCGTGCGCA GGAAGCCGAG CCCCCCAAGT CTGAGGAGAG TTTCAGATCT 
CGAGAGGAGG AAGAGGGCCA GGAAGGGCAG GATGCCATGT CCAGAGCCAA AGCCAACTGG 
TTGCGAGCCT TCAACAAGGT GCGCATGCAG CTGCAGGAGG CCCGAGGAGA AGGGGATATG 
TCTAAGTCTC TATGGTTCAA AGGCGGTCCT GGTGGTGGCC TTATCATCAT TGACAGCATG 
CCAGACATCA GGAAACGGAA GCCCATTCCC CTCGTGAGCG ACCTGGCTAT GTCATTGGTG 
CAGTCTCGGA AGGCAGGCAT CACCTCGGCC TTGGCCTCCA GCACGTTGAA CAATGAAGAG 
CTGAAAAATC ACGTTTACAA GAAGACCCTG CAAGCCTTAA TCTACCCCAT CTCCTGCACC 
ACGCCGCACA ATTTCGAGGT GTGGACGGCC ACCACGCCCA CCTACTGCTA TGAGTGCGAG 
GGGCTGCTGT GGGGCATCGC GCGGCAGGGC ATGCGATGCA CCGAGTGCGG CGTTAAGTGC 
CACGAGAAGT GCCAGGACCT GCTCAATGCG GATTGCCTGC AGCGGGCGGC TGAGAAGAGT 
TCCAAGCATG GCGCTGAAGA CCGCACGCAG AACATCATCA TGGTGCTGAA GGACCGCATG 
AAGATCCGCG AGCGCAACAA GCCTGAGATC TTCGAGCTTA TCCAAGAGAT CTTCGCGGTC 
ACCAAGAGCG CACACACACA GCAGATGAAA GCTGTCAAGC AGAGTGTGCT GGACGGCACA 
TCCAAGTGGT CTGCCAAAAT TAGCATCACA GTGGTCTGTG CCCAGGGCTT GCAGGCCAAG 
GACAAGACAG GATCCAGTGA CCCTTATGTC ACCGTCCAGG TTGGGAAGAC CAAGAAAAGG 
ACAAAAACCA TCTACGGGAA CCTCAACCCA GTGTGGGAAG AGAATTTTCA TTTTGAATGT 
CACAACTCCT CTGACCGGAT CAAGGTTCGT GTTTGGGATG AAGATGACGA CATAAAATCC 
CGTGTGAAAC AAAGGTTTAA GAGGGAGTCT GATGACTTCC TAGGGCAGAC AATCATCGAG 
GTGCGGACGC TTAGCGGCGA GATGGATGTG TGGTACAATC TGGACAAGAG AACGGACAAG 
TCGGCGGTGT CGGGGGCCAT CCGGCTTCAC ATCAGTGTGG AGATCAAAGG GGAGGAGAAG 
GTGGCGCCCT ACCATGTCCA GTACACCTGT CTGCATGAGA ACCTGTTCCA CTTTGTGACG 
GACGTGCAGA ACAATGGTGT GGTGAAGATT CCCGATGCCA AGGGTGACGA CGCCTGGAAG 
GTTTACTACG ATGAGACGGC CCAGGAGATC GTGGATGAGT TTGCCATGCG CTATGGTGTT 
GAGTCCATCT ACCAAGCCAT GACCCACTTT GCCTGCCTCT CCTCCAAGTA TATGTGCCCT 
GGGGTACCCG CTGTCATGAG CACCCTGCTT GCCAACATCA ACGCCTACTA CGCACACACC 
ACCGCCTCCA CCAACGTGTC TGCCTCTGAC CGCTTCGCTG CCTCTAATTT CGGGAAAGAG 
CGCTTTGTGA AACTTCTGGA CCAGCTACAC AATTCTCTGC GGATCGACCT GTCCATGTAC 
CGGAACAACT TCCCAGCCAG CAGCCCTGAG CGGCTGCAGG ATCTCAAGTC CACGGTGGAC 
CTGCTCACCA GCATCACCTT CTTCCGGATG AAGGTTCAGG AACTGCAGAG CCCGCCGCGT 
GCCAGCCAGG TAGTGAAAGA CTGTGTGAAG GCCTGCCTCA ACTCCACCTA TGAGTACATC 
TTCAACAACT GCCATGAGCT CTATGGCCGG GAGTACCAAA CGGATCCGGC CAAGAAGGGG 
GAGGTTCCCC CAGAGGAGCA AGGCCCTAGC ATCAAGAACC TGGATTTCTG GTCCAAGCTG 
ATCACCCTCA TCGTGTCTAT CATCGAGGAG GATAAGAATT CTTACACACC CTGCCTCAAT 
CAGTTTCCCC AGGAGCTCAA TGTGGGGAAG ATCAGTGCTG AGGTGATGTG GAGCCTATTT 
GCCCAAGACA TGAAGTACGC CATGGAGGAA CATGACAAAC ACCGGCTGTG TAAGAGCGCA 
GATTACATGA ACCTGCACTT CAAGGTGAAG TGGCTGTACA ATGAGTACGT GGCCGAGCTG 
CCCACCTTCA AGGACCGTGT GCCTGAGTAC CCTGCGTGGT TTGAGCCCTT CGTCATCCAG 
TGGTTGGATG AGAATGAGGA GGTGTCCCGG GACTTCCTGC ATGGTGCCCT CGAGCGGGAC 
AAGAAGGACG GGTTCCAGCA GACCTCAGAG CACGCCCTGT TCTCTTGCTC AGTGGTGGAC 
GTCTTCTCCC AGCTTAACCA GAGTTTCGAG ATCATCAAGA AGCTGGAGTG TCCTGACCCC 
CAGATCGTGG GCCATTACAT GCGGCGTTTT GCCAAGACCA TTAGCAATGT GCTTCTCCAG 
TATGCAGACA TCGTTTCCAA GGACTTCGCC TCCTACTGCT CCAAGGAGAA GGAGAAAGTG 
CCCTGCATCC TCATGAACAA CACACAGCAA CTACGAGTGC AGCTGGAGAA GATGTTTGAG 
GCGATGGGCG GGAAGGAGCT GGATGCCGAG GCCAGTGGCA CCCTGAAGGA GCTGCAGGTG 
AAACTCAACA ATGTCCTGGA TGAACTCAGC CATGTGTTTG CCACCAGCTT CCAGCCACAC 
ATTGAGGAAT GCGTCAGACA GATGGGTGAC ATCCTGAGCC AGGTGAAGGG CACAGGCAAT 
GTACCTGCCA GTGCCTGCAG CAGCGTGGCC CAGGATGCAG ACAATGTGCT GCAGCCCATC 
ATGGATCTTC TGGACAGCAA CCTCACCCTG TTTGCCAAAA TCTGTGAGAA GACAGTTCTG 
AAGCGGGTGC TGAAGGAGCT CTGGAAGCTG GTGATGAACA CAATGGAGAA GACCATCGTC 
CTGCCACCGC TCACTGACCA GACGGGGACA CAAATGATCT TCAATGCTGC CAAGGAGCTG 
GGCCAGCTGT CCAAACTTAA GGATCACATG GTGCGGGAAG AAGCCAAGAG CTTGACCCCG 
AAGCAGTGTG CTGTTGTTGA ACTGGCCCTG GACACCATCA AGCAATACTT CCACGCGGGG 
GGCGTTGGCC TCAAGAAGAC CTTCCTGGAG AAGAGCCCGG ACCTCCAGTC CCTGCGCTAC 
GCCCTGTCGC TCTACACGCA AGCCACCGAC CTGCTCATCA AAACCTTCGT GCAGACGCAG 
TCAGCGCAGG GCTCTGGTGT GGAGGACCCT GTAGGTGAAG TGTCCGTCCA CGTGGAGCTG 
TTCACACATC CAGGAACTGG GGAACAGAAG GTCACAGTGA AGGTGGTGGC CGCCAATGAC 
CTCAAGTGGC AGACTTCTGG CATCTTCCGC CCGTTCATCG AGGTCAACAT CGTTGGACCT 
CAGCTTAGCG ACAAGAAACG CAAGTTCGCC ACCAAATCCA AAAACAACAG CTGGGCGCCC 
AAGTATAACG AGAGCTTCCA GTTCTCCCTG AGCGCCGACG CGGGCCCCGA GTGCTACGAG 
CTGCAGGTGT GCGTGAAGGA CTACTGCTTT GCGCGCGAAG ACCGCACGGT GGGGCTGGCG 
GTGCTGCAAC TGCGAGAGCT GGCCCAGCGC GGGAGCGCCG CGTGCTGGCT TCCACTCGGC 
CGCCGCATCC ACATGGACGA CACGGGGCTC ACAGTGCTGC GCATCCTGTC GCAGCGCAGC 
AACGATGAGG TGGCCAAGGA ATTCGTCAAG CTCAAGTCGG ACACGCGCTC AGCCGAGGAG 
GGCGGTGCCG CGCCTGCGCC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Unc13a
Entrez Gene ID	382018
Full Name	unc-13 homolog A (C. elegans)
Synonyms	2410078G03Rik,Munc13-1
General protein information	Preferred Names unc-13 homolog A (C. elegans) Names protein unc-13 homolog A unc13a
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8\|8 B3.3

Bos taurus (cattle)	UNC13A	XP_002688619.2
Rattus norvegicus (Norway rat)	Unc13a	NP_074052.1
Danio rerio (zebrafish)	si:dkey-110c1.7	NP_001038630.1
Xenopus tropicalis (tropical clawed frog)	LOC100492289	XP_002938873.2
Homo sapiens (human)	UNC13A	NP_001073890.2
Gallus gallus (chicken)	LOC430487	XP_428042.4
Canis lupus familiaris (dog)	UNC13A	XP_005633256.1
Caenorhabditis elegans (roundworm)	unc-13	NP_001021874.1
Mus musculus (house mouse)	Unc13a	NP_001025044.2
Drosophila melanogaster (fruit fly)	unc-13	NP_651949.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Unc13a cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

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