Col27a1 cDNA ORF clone, Mus musculus(house mouse)

The following Col27a1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Col27a1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu81040 XM_017320286.1
Latest version!
Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OMu88569 XM_030253634.1
Latest version!
Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OMu33009 XM_006538039.4
Latest version!
Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu74976 XM_011250057.3
Latest version!
Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OMu74977 XM_011250058.3
Latest version!
Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu81039 XM_017320285.1
Latest version!
Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu88567 XM_030253632.1
Latest version!
Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu88568 XM_030253633.1
Latest version!
Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu33010 XM_006538040.3
Latest version!
Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OMu33005 XM_006538035.4
Latest version!
Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu81036 XM_017320282.1
Latest version!
Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu81037 XM_017320283.1
Latest version!
Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu81038 XM_017320284.1
Latest version!
Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu88564 XM_030253629.1
Latest version!
Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu88565 XM_030253630.1
Latest version!
Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu88566 XM_030253631.1
Latest version!
Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu20706 NM_025685.3
Latest version!
Mus musculus collagen, type XXVII, alpha 1 (Col27a1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
View Old Accession Versions >>
OMu33005 XM_006538035.3 Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OMu74977 XM_011250058.2 Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OMu74976 XM_011250057.2 Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OMu33009 XM_006538039.3 Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu81040
    Clone ID Related Accession (Same CDS sequence) XM_017320286.1
    Accession Version XM_017320286.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2886bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product collagen alpha-1(XXVII) chain isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG CCTGGACCTC CTGGGCAGCT GGGGCCTGAG 
    GGAGATGAAG GACCCATGGG GCCACCAGGA GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG
    AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT GGCTCGAAGG GGGAACCAGG GGATCCTGGA
    CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT ATGGGATTCA TTGGTCTTGT CGGAGAGCCT
    GGAATTGTTG GGGAGAAGGG AGACCGTGGG GTGATGGGAC CCCCAGGTGC ACCTGGACCC
    AAGGGGTCGA TGGGCCATCC TGGAACACCC GGTGGTATTG GGAACCCTGG AGAGCCTGGA
    CCCTGGGGCC CTCCAGGATC TCGAGGCCTG CCAGGCATGA GGGGAGCCAA GGGGCACCGG
    GGCCCTCGAG GACCTGATGG ACCAGCTGGG GAGCAGGGAT CCAAGGGCCT GAAGGGTCGT
    GTAGGACCTC GGGGCAGACC TGGCCAGCCA GGACAGCAGG GTGCAGCTGG TGAGCGAGGC
    CACTCAGGTG CAAAAGGCTT CCTTGGCATC CCTGGTCCCT CAGGCCCTCC AGGTGCCAAG
    GGCCTCCCAG GAGAACCGGG CTCTCAGGGA CCTCAGGGGC CAGTTGGTCC TCCAGGAGAA
    ATGGGGCCCA AGGGACCGCC GGGTGCAGTG GGAGAGCCTG GCCTTCCTGG GGACTCCGGA
    ATGAAGGGTG ACCTCGGACC TCTGGGCCCC CCTGGGGAGC AAGGTCTTAT TGGGCAGCGG
    GGAGAGCCAG GCCTTGAGGG AGACCATGGC CCTGTGGGGC CAGATGGGCT GAAGGGCGAC
    AGGGGCGACC CAGGGCCCGA TGGCGAGCAT GGTGAAAAGG GCCAGGAAGG GCTGAAAGGA
    GAGGATGGGT CCCCTGGGCC CCCGGGCATC ACTGGCGTCC CGGGTCGTGA GGGGAAGCCG
    GGCAAGCAGG GTGAGAAAGG CCAGAGGGGA GCTAAGGGTG CCAAGGGCCA CCAAGGATAT
    CTGGGAGAGA TGGGCATCCC CGGAGAACCT GGGCCCCCTG GGACCCCAGG TCCTAAAGGG
    TCCCGGGGCA CCCTAGGACC AACGGGTGCT CCAGGACGGA TGGGAGCCCA AGGAGAACCA
    GGATTGGCCG GTTATAATGG ACACAAAGGC ATCACAGGAC CCCTCGGGCC TCCCGGGCCC
    AAAGGCGAGA AGGGGGACCA GGGCGAGGAT GGCAAGACCG AGGGGCCCCC TGGGCCACCA
    GGAGATCGGG GTCCTGTGGG TGATCGAGGA GACCGTGGGG AGCCAGGCGA CCCTGGATAC
    CCTGGTCAAG AGGGAGTTCA AGGCCTCCGT GGAGAACCGG GCCAGCAGGG ACAGCCTGGG
    CATCCAGGAC CCCGGGGGCG CCCAGGACCC AAAGGATCAA AAGGCGAAGA GGGCCCAAAG
    GGAAAGCCAG GCAAGGCTGG GCCATCAGGC CGGAGGGGGA CCCAGGTGAG TTGCCATAGC
    TTCTCTGTCA CCTTCTATGT TCTCCTGAGA AGGTTTAACC AGCTTCTTCT GTACCTCCAG
    GGCCTTCAAG GACTGCCAGG CCCCCGAGGC GTGGTGGGGA GACAGGGCCC TGAGGGCACT
    GCTGGATCTG ATGGGATTCC TGGCAGAGAT GGTCGGCCAG GATATCAGGG AGACCAGGGA
    AATGATGGGG ACCCTGGCCC TGTGGGCCCT GCTGGGAGAA GAGGAAATCC AGGTGTGGCT
    GGCTTGCCTG GAGCACAGGG GCCTCCGGGA TTCAAGGGTG AAAGTGGGTT ACCTGGGCAA
    CTGGGTCCCC CTGGAAAACG AGGGACAGAA GGTGGAACAG GGCTTCCTGG GAACCAGGGG
    GAGCCAGGAT CCAAAGGCCA GCCGGGTGAC TCTGGCGAGA TGGGCTTCCC AGGAGTGGCT
    GGCCTCTTTG GACCCAAGGG CCCCCCTGGA GACATTGGCT TCAAAGGCAT CCAAGGTCCT
    CGGGGGCCTC CTGGCTTGAT GGGAAAGGAA GGTATCATTG GCCCCCCCGG AATGCTGGGA
    CCTTCTGGAC TCCCGGGTCC CAAAGGTGAC AGAGGCAGCC GAGGAGACTT GGGACTGCAA
    GGCCCAAGGG GTCCTCCTGG TCCAAGGGGC CGGCCAGGTC CCCCGGGCCC TCCTTGGCAT
    CCCATCCAGT TTCAGCAAGA TGACCTTGGA GCAGCTTTCC AGACATGGAT GGATGCTCAA
    GGAGCCGTCA GATCGGAGCA GGGGTACAGC TATCCGGACC AGCTGGCGCT AGACCAGGGA
    GGGGAGATCT TCAAAACCTT ACACTACCTC AGCAACCTCA TCCAGAGCAT TAAGACACCC
    TTGGGCACCA AGGAGAACCC AGCCCGGGTC TGCCGGGACC TCATGGACTG TGAGCAGAGG
    ATGGCGGATG GTACCTACTG GGTGGACCCC AACCTCGGCT GCTCCTCTGA CACCATTGAA
    GTCTCCTGCA ACTTTACACA GGGTGGGCAG ACGTGCCTGA AGCCCATCAC GGCCTCCAAG
    GCCGAGTTTG CTGTGAGTCG GGTCCAGATG AATTTCTTGC ACCTGCTGAG CTCTGAGGGG
    ACACAGCATA TCACAATCCA CTGTCTGAAC ATGACGGTGT GGCAGGAGGG ACCGGGACGC
    TCCTCTGCCA GACAGGCTGT GCGCTTCCGT GCCTGGAACG GACAGGTCTT CGAAGCTGGG
    GGTCAGTTCA GGCCAGAGGT GTCTATGGAT GGCTGCAAGG TCCATGATGG CCGCTGGCAT
    CAGACACTGT TCACCTTCCG GACCCAGGAC CCCCAGCAGC TGCCCATCGT CAGTGTGGAC
    AATCTCCCGC CTGTCTCATC AGGGAAGCAG TACCGCCTGG AAGTTGGACC TGCGTGCTTC
    CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017175775.1
    CDS1532..4417
    Translation

    Target ORF information:

    RefSeq Version XM_017320286.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017320286.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG CCTGGACCTC CTGGGCAGCT GGGGCCTGAG 
    GGAGATGAAG GACCCATGGG GCCACCAGGA GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG
    AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT GGCTCGAAGG GGGAACCAGG GGATCCTGGA
    CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT ATGGGATTCA TTGGTCTTGT CGGAGAGCCT
    GGAATTGTTG GGGAGAAGGG AGACCGTGGG GTGATGGGAC CCCCAGGTGC ACCTGGACCC
    AAGGGGTCGA TGGGCCATCC TGGAACACCC GGTGGTATTG GGAACCCTGG AGAGCCTGGA
    CCCTGGGGCC CTCCAGGATC TCGAGGCCTG CCAGGCATGA GGGGAGCCAA GGGGCACCGG
    GGCCCTCGAG GACCTGATGG ACCAGCTGGG GAGCAGGGAT CCAAGGGCCT GAAGGGTCGT
    GTAGGACCTC GGGGCAGACC TGGCCAGCCA GGACAGCAGG GTGCAGCTGG TGAGCGAGGC
    CACTCAGGTG CAAAAGGCTT CCTTGGCATC CCTGGTCCCT CAGGCCCTCC AGGTGCCAAG
    GGCCTCCCAG GAGAACCGGG CTCTCAGGGA CCTCAGGGGC CAGTTGGTCC TCCAGGAGAA
    ATGGGGCCCA AGGGACCGCC GGGTGCAGTG GGAGAGCCTG GCCTTCCTGG GGACTCCGGA
    ATGAAGGGTG ACCTCGGACC TCTGGGCCCC CCTGGGGAGC AAGGTCTTAT TGGGCAGCGG
    GGAGAGCCAG GCCTTGAGGG AGACCATGGC CCTGTGGGGC CAGATGGGCT GAAGGGCGAC
    AGGGGCGACC CAGGGCCCGA TGGCGAGCAT GGTGAAAAGG GCCAGGAAGG GCTGAAAGGA
    GAGGATGGGT CCCCTGGGCC CCCGGGCATC ACTGGCGTCC CGGGTCGTGA GGGGAAGCCG
    GGCAAGCAGG GTGAGAAAGG CCAGAGGGGA GCTAAGGGTG CCAAGGGCCA CCAAGGATAT
    CTGGGAGAGA TGGGCATCCC CGGAGAACCT GGGCCCCCTG GGACCCCAGG TCCTAAAGGG
    TCCCGGGGCA CCCTAGGACC AACGGGTGCT CCAGGACGGA TGGGAGCCCA AGGAGAACCA
    GGATTGGCCG GTTATAATGG ACACAAAGGC ATCACAGGAC CCCTCGGGCC TCCCGGGCCC
    AAAGGCGAGA AGGGGGACCA GGGCGAGGAT GGCAAGACCG AGGGGCCCCC TGGGCCACCA
    GGAGATCGGG GTCCTGTGGG TGATCGAGGA GACCGTGGGG AGCCAGGCGA CCCTGGATAC
    CCTGGTCAAG AGGGAGTTCA AGGCCTCCGT GGAGAACCGG GCCAGCAGGG ACAGCCTGGG
    CATCCAGGAC CCCGGGGGCG CCCAGGACCC AAAGGATCAA AAGGCGAAGA GGGCCCAAAG
    GGAAAGCCAG GCAAGGCTGG GCCATCAGGC CGGAGGGGGA CCCAGGTGAG TTGCCATAGC
    TTCTCTGTCA CCTTCTATGT TCTCCTGAGA AGGTTTAACC AGCTTCTTCT GTACCTCCAG
    GGCCTTCAAG GACTGCCAGG CCCCCGAGGC GTGGTGGGGA GACAGGGCCC TGAGGGCACT
    GCTGGATCTG ATGGGATTCC TGGCAGAGAT GGTCGGCCAG GATATCAGGG AGACCAGGGA
    AATGATGGGG ACCCTGGCCC TGTGGGCCCT GCTGGGAGAA GAGGAAATCC AGGTGTGGCT
    GGCTTGCCTG GAGCACAGGG GCCTCCGGGA TTCAAGGGTG AAAGTGGGTT ACCTGGGCAA
    CTGGGTCCCC CTGGAAAACG AGGGACAGAA GGTGGAACAG GGCTTCCTGG GAACCAGGGG
    GAGCCAGGAT CCAAAGGCCA GCCGGGTGAC TCTGGCGAGA TGGGCTTCCC AGGAGTGGCT
    GGCCTCTTTG GACCCAAGGG CCCCCCTGGA GACATTGGCT TCAAAGGCAT CCAAGGTCCT
    CGGGGGCCTC CTGGCTTGAT GGGAAAGGAA GGTATCATTG GCCCCCCCGG AATGCTGGGA
    CCTTCTGGAC TCCCGGGTCC CAAAGGTGAC AGAGGCAGCC GAGGAGACTT GGGACTGCAA
    GGCCCAAGGG GTCCTCCTGG TCCAAGGGGC CGGCCAGGTC CCCCGGGCCC TCCTTGGCAT
    CCCATCCAGT TTCAGCAAGA TGACCTTGGA GCAGCTTTCC AGACATGGAT GGATGCTCAA
    GGAGCCGTCA GATCGGAGCA GGGGTACAGC TATCCGGACC AGCTGGCGCT AGACCAGGGA
    GGGGAGATCT TCAAAACCTT ACACTACCTC AGCAACCTCA TCCAGAGCAT TAAGACACCC
    TTGGGCACCA AGGAGAACCC AGCCCGGGTC TGCCGGGACC TCATGGACTG TGAGCAGAGG
    ATGGCGGATG GTACCTACTG GGTGGACCCC AACCTCGGCT GCTCCTCTGA CACCATTGAA
    GTCTCCTGCA ACTTTACACA GGGTGGGCAG ACGTGCCTGA AGCCCATCAC GGCCTCCAAG
    GCCGAGTTTG CTGTGAGTCG GGTCCAGATG AATTTCTTGC ACCTGCTGAG CTCTGAGGGG
    ACACAGCATA TCACAATCCA CTGTCTGAAC ATGACGGTGT GGCAGGAGGG ACCGGGACGC
    TCCTCTGCCA GACAGGCTGT GCGCTTCCGT GCCTGGAACG GACAGGTCTT CGAAGCTGGG
    GGTCAGTTCA GGCCAGAGGT GTCTATGGAT GGCTGCAAGG TCCATGATGG CCGCTGGCAT
    CAGACACTGT TCACCTTCCG GACCCAGGAC CCCCAGCAGC TGCCCATCGT CAGTGTGGAC
    AATCTCCCGC CTGTCTCATC AGGGAAGCAG TACCGCCTGG AAGTTGGACC TGCGTGCTTC
    CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu88569
    Clone ID Related Accession (Same CDS sequence) XM_030253634.1
    Accession Version XM_030253634.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2811bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product collagen alpha-1(XXVII) chain isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG CCTGGACCTC CTGGGCAGCT GGGGCCTGAG 
    GGAGATGAAG GACCCATGGG GCCACCAGGA GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG
    AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT GGCTCGAAGG GGGAACCAGG GGATCCTGGA
    CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT ATGGGATTCA TTGGTCTTGT CGGAGAGCCT
    GGAATTGTTG GGGAGAAGGG AGACCGTGGG GTGATGGGAC CCCCAGGTGC ACCTGGACCC
    AAGGGGTCGA TGGGCCATCC TGGAACACCC GGTGGTATTG GGAACCCTGG AGAGCCTGGA
    CCCTGGGGCC CTCCAGGATC TCGAGGCCTG CCAGGCATGA GGGGAGCCAA GGGGCACCGG
    GGCCCTCGAG GACCTGATGG ACCAGCTGGG GAGCAGGGAT CCAAGGGCCT GAAGGGTCGT
    GTAGGACCTC GGGGCAGACC TGGCCAGCCA GGACAGCAGG GTGCAGCTGG TGAGCGAGGC
    CACTCAGGTG CAAAAGGCTT CCTTGGCATC CCTGGTCCCT CAGGCCCTCC AGGTGCCAAG
    GGCCTCCCAG GAGAACCGGG CTCTCAGGGA CCTCAGGGGC CAGTTGGTCC TCCAGGAGAA
    ATGGGGCCCA AGGGACCGCC GGGTGCAGTG GGAGAGCCTG GCCTTCCTGG GGACTCCGGA
    ATGAAGGGTG ACCTCGGACC TCTGGGCCCC CCTGGGGAGC AAGGTCTTAT TGGGCAGCGG
    GGAGAGCCAG GCCTTGAGGG AGACCATGGC CCTGTGGGGC CAGATGGGCT GAAGGGCGAC
    AGGGGCGACC CAGGGCCCGA TGGCGAGCAT GGTGAAAAGG GCCAGGAAGG GCTGAAAGGA
    GAGGATGGGT CCCCTGGGCC CCCGGGCATC ACTGGCGTCC CGGGTCGTGA GGGGAAGCCG
    GGCAAGCAGG GTGAGAAAGG CCAGAGGGGA GCTAAGGGTG CCAAGGGCCA CCAAGGATAT
    CTGGGAGAGA TGGGCATCCC CGGAGAACCT GGGCCCCCTG GGACCCCAGG TCCTAAAGGG
    TCCCGGGGCA CCCTAGGACC AACGGGTGCT CCAGGACGGA TGGGAGCCCA AGGAGAACCA
    GGATTGGCCG GTTATAATGG ACACAAAGGC ATCACAGGAC CCCTCGGGCC TCCCGGGCCC
    AAAGGCGAGA AGGGGGACCA GGGCGAGGAT GGCAAGACCG AGGGGCCCCC TGGGCCACCA
    GGAGATCGGG GTCCTGTGGG TGATCGAGGA GACCGTGGGG AGCCAGGCGA CCCTGGATAC
    CCTGGTCAAG AGGGAGTTCA AGGCCTCCGT GGAGAACCGG GCCAGCAGGG ACAGCCTGGG
    CATCCAGGAC CCCGGGGGCG CCCAGGACCC AAAGGATCAA AAGGCGAAGA GGGCCCAAAG
    GGAAAGCCAG GCAAGGCTGG GCCATCAGGC CGGAGGGGGA CCCAGGGCCT TCAAGGACTG
    CCAGGCCCCC GAGGCGTGGT GGGGAGACAG GGCCCTGAGG GCACTGCTGG ATCTGATGGG
    ATTCCTGGCA GAGATGGTCG GCCAGGATAT CAGGGAGACC AGGGAAATGA TGGGGACCCT
    GGCCCTGTGG GCCCTGCTGG GAGAAGAGGA AATCCAGGTG TGGCTGGCTT GCCTGGAGCA
    CAGGGGCCTC CGGGATTCAA GGGTGAAAGT GGGTTACCTG GGCAACTGGG TCCCCCTGGA
    AAACGAGGGA CAGAAGGTGG AACAGGGCTT CCTGGGAACC AGGGGGAGCC AGGATCCAAA
    GGCCAGCCGG GTGACTCTGG CGAGATGGGC TTCCCAGGAG TGGCTGGCCT CTTTGGACCC
    AAGGGCCCCC CTGGAGACAT TGGCTTCAAA GGCATCCAAG GTCCTCGGGG GCCTCCTGGC
    TTGATGGGAA AGGAAGGTAT CATTGGCCCC CCCGGAATGC TGGGACCTTC TGGACTCCCG
    GGTCCCAAAG GTGACAGAGG CAGCCGAGGA GACTTGGGAC TGCAAGGCCC AAGGGGTCCT
    CCTGGTCCAA GGGGCCGGCC AGGTCCCCCG GGCCCTCCTT GGCATCCCAT CCAGTTTCAG
    CAAGATGACC TTGGAGCAGC TTTCCAGACA TGGATGGATG CTCAAGGAGC CGTCAGATCG
    GAGCAGGGGT ACAGCTATCC GGACCAGCTG GCGCTAGACC AGGGAGGGGA GATCTTCAAA
    ACCTTACACT ACCTCAGCAA CCTCATCCAG AGCATTAAGA CACCCTTGGG CACCAAGGAG
    AACCCAGCCC GGGTCTGCCG GGACCTCATG GACTGTGAGC AGAGGATGGC GGATGGTACC
    TACTGGGTGG ACCCCAACCT CGGCTGCTCC TCTGACACCA TTGAAGTCTC CTGCAACTTT
    ACACAGGGTG GGCAGACGTG CCTGAAGCCC ATCACGGCCT CCAAGGCCGA GTTTGCTGTG
    AGTCGGGTCC AGATGAATTT CTTGCACCTG CTGAGCTCTG AGGGGACACA GCATATCACA
    ATCCACTGTC TGAACATGAC GGTGTGGCAG GAGGGACCGG GACGCTCCTC TGCCAGACAG
    GCTGTGCGCT TCCGTGCCTG GAACGGACAG GTCTTCGAAG CTGGGGGTCA GTTCAGGCCA
    GAGGTGTCTA TGGATGGCTG CAAGGTCCAT GATGGCCGCT GGCATCAGAC ACTGTTCACC
    TTCCGGACCC AGGACCCCCA GCAGCTGCCC ATCGTCAGTG TGGACAATCT CCCGCCTGTC
    TCATCAGGGA AGCAGTACCG CCTGGAAGTT GGACCTGCGT GCTTCCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030109494.1
    CDS545..3355
    Translation

    Target ORF information:

    RefSeq Version XM_030253634.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030253634.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG CCTGGACCTC CTGGGCAGCT GGGGCCTGAG 
    GGAGATGAAG GACCCATGGG GCCACCAGGA GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG
    AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT GGCTCGAAGG GGGAACCAGG GGATCCTGGA
    CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT ATGGGATTCA TTGGTCTTGT CGGAGAGCCT
    GGAATTGTTG GGGAGAAGGG AGACCGTGGG GTGATGGGAC CCCCAGGTGC ACCTGGACCC
    AAGGGGTCGA TGGGCCATCC TGGAACACCC GGTGGTATTG GGAACCCTGG AGAGCCTGGA
    CCCTGGGGCC CTCCAGGATC TCGAGGCCTG CCAGGCATGA GGGGAGCCAA GGGGCACCGG
    GGCCCTCGAG GACCTGATGG ACCAGCTGGG GAGCAGGGAT CCAAGGGCCT GAAGGGTCGT
    GTAGGACCTC GGGGCAGACC TGGCCAGCCA GGACAGCAGG GTGCAGCTGG TGAGCGAGGC
    CACTCAGGTG CAAAAGGCTT CCTTGGCATC CCTGGTCCCT CAGGCCCTCC AGGTGCCAAG
    GGCCTCCCAG GAGAACCGGG CTCTCAGGGA CCTCAGGGGC CAGTTGGTCC TCCAGGAGAA
    ATGGGGCCCA AGGGACCGCC GGGTGCAGTG GGAGAGCCTG GCCTTCCTGG GGACTCCGGA
    ATGAAGGGTG ACCTCGGACC TCTGGGCCCC CCTGGGGAGC AAGGTCTTAT TGGGCAGCGG
    GGAGAGCCAG GCCTTGAGGG AGACCATGGC CCTGTGGGGC CAGATGGGCT GAAGGGCGAC
    AGGGGCGACC CAGGGCCCGA TGGCGAGCAT GGTGAAAAGG GCCAGGAAGG GCTGAAAGGA
    GAGGATGGGT CCCCTGGGCC CCCGGGCATC ACTGGCGTCC CGGGTCGTGA GGGGAAGCCG
    GGCAAGCAGG GTGAGAAAGG CCAGAGGGGA GCTAAGGGTG CCAAGGGCCA CCAAGGATAT
    CTGGGAGAGA TGGGCATCCC CGGAGAACCT GGGCCCCCTG GGACCCCAGG TCCTAAAGGG
    TCCCGGGGCA CCCTAGGACC AACGGGTGCT CCAGGACGGA TGGGAGCCCA AGGAGAACCA
    GGATTGGCCG GTTATAATGG ACACAAAGGC ATCACAGGAC CCCTCGGGCC TCCCGGGCCC
    AAAGGCGAGA AGGGGGACCA GGGCGAGGAT GGCAAGACCG AGGGGCCCCC TGGGCCACCA
    GGAGATCGGG GTCCTGTGGG TGATCGAGGA GACCGTGGGG AGCCAGGCGA CCCTGGATAC
    CCTGGTCAAG AGGGAGTTCA AGGCCTCCGT GGAGAACCGG GCCAGCAGGG ACAGCCTGGG
    CATCCAGGAC CCCGGGGGCG CCCAGGACCC AAAGGATCAA AAGGCGAAGA GGGCCCAAAG
    GGAAAGCCAG GCAAGGCTGG GCCATCAGGC CGGAGGGGGA CCCAGGGCCT TCAAGGACTG
    CCAGGCCCCC GAGGCGTGGT GGGGAGACAG GGCCCTGAGG GCACTGCTGG ATCTGATGGG
    ATTCCTGGCA GAGATGGTCG GCCAGGATAT CAGGGAGACC AGGGAAATGA TGGGGACCCT
    GGCCCTGTGG GCCCTGCTGG GAGAAGAGGA AATCCAGGTG TGGCTGGCTT GCCTGGAGCA
    CAGGGGCCTC CGGGATTCAA GGGTGAAAGT GGGTTACCTG GGCAACTGGG TCCCCCTGGA
    AAACGAGGGA CAGAAGGTGG AACAGGGCTT CCTGGGAACC AGGGGGAGCC AGGATCCAAA
    GGCCAGCCGG GTGACTCTGG CGAGATGGGC TTCCCAGGAG TGGCTGGCCT CTTTGGACCC
    AAGGGCCCCC CTGGAGACAT TGGCTTCAAA GGCATCCAAG GTCCTCGGGG GCCTCCTGGC
    TTGATGGGAA AGGAAGGTAT CATTGGCCCC CCCGGAATGC TGGGACCTTC TGGACTCCCG
    GGTCCCAAAG GTGACAGAGG CAGCCGAGGA GACTTGGGAC TGCAAGGCCC AAGGGGTCCT
    CCTGGTCCAA GGGGCCGGCC AGGTCCCCCG GGCCCTCCTT GGCATCCCAT CCAGTTTCAG
    CAAGATGACC TTGGAGCAGC TTTCCAGACA TGGATGGATG CTCAAGGAGC CGTCAGATCG
    GAGCAGGGGT ACAGCTATCC GGACCAGCTG GCGCTAGACC AGGGAGGGGA GATCTTCAAA
    ACCTTACACT ACCTCAGCAA CCTCATCCAG AGCATTAAGA CACCCTTGGG CACCAAGGAG
    AACCCAGCCC GGGTCTGCCG GGACCTCATG GACTGTGAGC AGAGGATGGC GGATGGTACC
    TACTGGGTGG ACCCCAACCT CGGCTGCTCC TCTGACACCA TTGAAGTCTC CTGCAACTTT
    ACACAGGGTG GGCAGACGTG CCTGAAGCCC ATCACGGCCT CCAAGGCCGA GTTTGCTGTG
    AGTCGGGTCC AGATGAATTT CTTGCACCTG CTGAGCTCTG AGGGGACACA GCATATCACA
    ATCCACTGTC TGAACATGAC GGTGTGGCAG GAGGGACCGG GACGCTCCTC TGCCAGACAG
    GCTGTGCGCT TCCGTGCCTG GAACGGACAG GTCTTCGAAG CTGGGGGTCA GTTCAGGCCA
    GAGGTGTCTA TGGATGGCTG CAAGGTCCAT GATGGCCGCT GGCATCAGAC ACTGTTCACC
    TTCCGGACCC AGGACCCCCA GCAGCTGCCC ATCGTCAGTG TGGACAATCT CCCGCCTGTC
    TCATCAGGGA AGCAGTACCG CCTGGAAGTT GGACCTGCGT GCTTCCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu33009
    Clone ID Related Accession (Same CDS sequence) XM_006538039.3 , XM_006538039.4
    Accession Version XM_006538039.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3186bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product collagen alpha-1(XXVII) chain isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006538039.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGGCACGG GATTCGCGCG GGGGGCCCGA GGCACAGCGG CGTCAGGACC CGGGGGGGGG 
    TTTCTCTTCG CCTGGATCTT GGTCTCATTT ACCTGTCACC TGGCCTCCAC CCAAGGAGCT
    CCTGAAGATG TGGATGTCCT CCAGCGGCTG GGCCTCAGCT GGACGAAGGC CGGGGGTGGC
    CGGAGTCCTA CACCCCCTGG TGTCATTCCT TTCCCATCTG GCTTCATCTT CACACAGCGG
    GCCAAGCTGC AGGCCCCCAC AGCCAATGTT CTTCCCACCA CCCTGGGGCG GGAGCTGGCA
    TTGGTGTTGA GCCTCTGTTC ACATCGTGTG AACCATGCCT TCCTCTTTGC CATCCGCAGC
    AGGAAACACA AGCTGCAACT GGGTCTGCAG TTCCTCCCCG GCAGGACGAT CATCCACCTG
    GGGCCTCGGC AATCAGTGGC CTTCGACTTG GATGTGCATG ATGGGCGCTG GCACCACCTG
    GCCCTAGAGT TGCGTGGCCG CACAGTCACA ATGGTGACAG CCTGTGGGCA GCACAGGGTA
    CCTGTCCCAC TGCCTTCCCG TAGGGACTCC ATGCTTGACC CCCAGGGCTC CTTTCTCTTG
    GGGAAGGTGA ACCCCCGTGC TGTCCAGTTT GAAGGTGCAC TCTGTCAGTT CAGCATCCAC
    CCTGTGGCGC AGGTCGCTCA CAATTACTGT GCCCACCTGA GAGAGCGGTG CCGACAGGTG
    GACACATATA GTCCCCAGGT GGGAACCCTC TTTCCTTGGG ACTCTGGCCC AGCCTTTGCT
    TTGCATCCTG AACCAGCCTT GCTTGGCTTG GGGAATCTGA CCAGAACTCC AGCAACCCTG
    GGGGCCAGGC CTGTAAGCAG GGCACTTGCA GTGACTCTGG CTCCTGCCAT GCCCACCAAG
    CCCCTGAGGA CTGTCCACCC AGATGTCTCT GAACACAGCT CTTCCCAGAC CCCACTGTCA
    CCTGCCAAAC AGTCGGCCAG GAAAACACCT TCCCCCTCCT CGTCAGCTTC CCTGGCTAAT
    TCCACCAGGG TTTATCGTCC AGCTGCTGCC CAGCCAAGAC AGATCACAAC TACTAGCCCC
    ACCAAACGTT CCCCCACCAA GCCTTCTGTT TCTCCCCTTT CAGTTACCCC CATGAAAAGT
    CCCCATGCAA CCCAGAAAAC AGGTGTCCCT TCATTCACAA AGCCTGTTCC ACCCACTCAG
    AAGCCAGCGC CCTTCACCTC CTACCTAGCA CCTTCCAAAG CCTCTAGTCC CACTGTGAGG
    CCTGTTCAGA AGACTTTCAT GACACCCCGA CCTCCAGTCC CCAGCCCCCA GCCCCTGCGC
    CCTACTACTG GCTTATCTAA GAAGTTCACT AACCCCACTG TTGCAAAATC TAAGTCCAAG
    ACGACCAGTT GGGCCAGCAA GCCGGTCTTG GCCCGCTCCA GTGTCCCTAA AACGCTTCAG
    CAAACTGTTT TGTCCCAGTC TCCTGTTTCC TATCTGGGCT CTCAGACACT GGCTCCAGCC
    CTCCCTCCTT TGGGTGTTGG AAATCCTAGA ACGATGCCTC CCACACGTGA CTCTGCTTTA
    ACTCCTGCTG GAAGCAAGAA ATTCACAGGA CGGGAAACCT CCAAGAAAAC CAGACAGAAG
    AGCAGCCCCC GGAAGCCCGA ACCTCTCAGC CCTGGGAAGT CAGCCAGGGA TGCCTCACCA
    AGAGACCTGA CAACCAAGCC TAGCCGGCCG TCCACCCCAG CCCTGGTCCT TGCCCCAGCT
    TACCTCCTGT CCTCCAGCCC CCAGCCCACC AGCAGCAGCT TCCCTTTCTT CCACCTGCTG
    GGCCCTACAC CTTTCCCGAT GCTGATGGGG CCTCCAGGCT CCAAGGGAGA CTGTGGCTTG
    CCGGGTCCCC CTGGGCTTCC AGGATTACCT GGATCTCCGG GCGCACGAGG TCCTCGGGGT
    CCTCCTGGGC CGTACGGGAA CCCAGGCCCA CCTGGCCCTC CAGGAGCCAA AGGACAGAAA
    GGGGACCCAG GACTCTCACC AGGACAGGCT CACGATGGAG CAAAGGGCAA CATGGGCTTG
    CCTGGGCTCT CCGGAAATCC AGGACCCTTG GGAAGGAAGG GACACAAGGG CCATCCTGGA
    GCAGCAGGGC ACCCTGGAGA ACAGGGGCAG CCAGGACCTG AGGGCAGTCC AGGGGCCAAA
    GGTTACCCTG GCAGGCAGGG GTTCCCTGGA CCTGTAGGAG ACCCTGGCCC CAAAGGCAGC
    AGGGGTTACA TTGGTCTTCC GGGGCTCTTT GGCCTGCCAG GGTCGGATGG AGAGCGGGGT
    CTCCCTGGCG TTCCTGGCAA GAGGGGCGAG ATGGGTAGGC CGGGCTTCCC TGGAGACTTT
    GGGGAAAGAG GCCCCCCTGG ACTTGATGGC AACCCTGGAG AAATTGGCCT GCCGGGGCCA
    CCAGGAGTGC TCGGGCTCAT TGGTGACACA GGAGCACTGG GCCCAGTTGG CTACCCAGGA
    CCGAAAGGCA TGAAGGGACT GATGGGCGGC GTGGGGGAGC CCGGACTGAA AGGTGATAAG
    GGTGAACAAG GGGTGCCAGG TGTGTCGGGA GATCCTGGCT TCCAAGGAGA CAAGGGGAGT
    CATGGCTTGC CAGGGCTCCC GGGTGGCCGG GGGAAGCCAG GGCCTCTGGG AAAAGCTGGA
    GACAAAGGAT CACTTGGGTT TCCTGGACCT CCTGGACCTG AGGGTTTCCC AGGAGACATT
    GGCCCTCCAG GGGACAATGG CCCTGAAGGC ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG
    CCTGGACCTC CTGGGCAGCT GGGGCCTGAG GGAGATGAAG GACCCATGGG GCCACCAGGA
    GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT
    GGCTCGAAGG GGGAACCAGG GGATCCTGGA CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT
    ATGGGATTCA TTGGTCTTGT CGGAGAGCCT GGAATTGTTG GGGAGAAGGG AGACCGTGGG
    GTGATGGGAC CCCCAGGTGC ACCTGGACCC AAGGGGTCGA TGGTAAGGAG GCCATCCTGG
    AACACCCGGT GGTATTGGGA ACCCTGGAGA GCCTGGACCC TGGGGCCCTC CAGGATCTCG
    AGGCCTGCCA GGCATGAGGG GAGCCAAGGG GCACCGGGGT CTCCAGCTGA CTTACACAGG
    CTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006538102.1
    CDS507..3692
    Translation

    Target ORF information:

    RefSeq Version XM_006538039.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006538039.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGGCACGG GATTCGCGCG GGGGGCCCGA GGCACAGCGG CGTCAGGACC CGGGGGGGGG 
    TTTCTCTTCG CCTGGATCTT GGTCTCATTT ACCTGTCACC TGGCCTCCAC CCAAGGAGCT
    CCTGAAGATG TGGATGTCCT CCAGCGGCTG GGCCTCAGCT GGACGAAGGC CGGGGGTGGC
    CGGAGTCCTA CACCCCCTGG TGTCATTCCT TTCCCATCTG GCTTCATCTT CACACAGCGG
    GCCAAGCTGC AGGCCCCCAC AGCCAATGTT CTTCCCACCA CCCTGGGGCG GGAGCTGGCA
    TTGGTGTTGA GCCTCTGTTC ACATCGTGTG AACCATGCCT TCCTCTTTGC CATCCGCAGC
    AGGAAACACA AGCTGCAACT GGGTCTGCAG TTCCTCCCCG GCAGGACGAT CATCCACCTG
    GGGCCTCGGC AATCAGTGGC CTTCGACTTG GATGTGCATG ATGGGCGCTG GCACCACCTG
    GCCCTAGAGT TGCGTGGCCG CACAGTCACA ATGGTGACAG CCTGTGGGCA GCACAGGGTA
    CCTGTCCCAC TGCCTTCCCG TAGGGACTCC ATGCTTGACC CCCAGGGCTC CTTTCTCTTG
    GGGAAGGTGA ACCCCCGTGC TGTCCAGTTT GAAGGTGCAC TCTGTCAGTT CAGCATCCAC
    CCTGTGGCGC AGGTCGCTCA CAATTACTGT GCCCACCTGA GAGAGCGGTG CCGACAGGTG
    GACACATATA GTCCCCAGGT GGGAACCCTC TTTCCTTGGG ACTCTGGCCC AGCCTTTGCT
    TTGCATCCTG AACCAGCCTT GCTTGGCTTG GGGAATCTGA CCAGAACTCC AGCAACCCTG
    GGGGCCAGGC CTGTAAGCAG GGCACTTGCA GTGACTCTGG CTCCTGCCAT GCCCACCAAG
    CCCCTGAGGA CTGTCCACCC AGATGTCTCT GAACACAGCT CTTCCCAGAC CCCACTGTCA
    CCTGCCAAAC AGTCGGCCAG GAAAACACCT TCCCCCTCCT CGTCAGCTTC CCTGGCTAAT
    TCCACCAGGG TTTATCGTCC AGCTGCTGCC CAGCCAAGAC AGATCACAAC TACTAGCCCC
    ACCAAACGTT CCCCCACCAA GCCTTCTGTT TCTCCCCTTT CAGTTACCCC CATGAAAAGT
    CCCCATGCAA CCCAGAAAAC AGGTGTCCCT TCATTCACAA AGCCTGTTCC ACCCACTCAG
    AAGCCAGCGC CCTTCACCTC CTACCTAGCA CCTTCCAAAG CCTCTAGTCC CACTGTGAGG
    CCTGTTCAGA AGACTTTCAT GACACCCCGA CCTCCAGTCC CCAGCCCCCA GCCCCTGCGC
    CCTACTACTG GCTTATCTAA GAAGTTCACT AACCCCACTG TTGCAAAATC TAAGTCCAAG
    ACGACCAGTT GGGCCAGCAA GCCGGTCTTG GCCCGCTCCA GTGTCCCTAA AACGCTTCAG
    CAAACTGTTT TGTCCCAGTC TCCTGTTTCC TATCTGGGCT CTCAGACACT GGCTCCAGCC
    CTCCCTCCTT TGGGTGTTGG AAATCCTAGA ACGATGCCTC CCACACGTGA CTCTGCTTTA
    ACTCCTGCTG GAAGCAAGAA ATTCACAGGA CGGGAAACCT CCAAGAAAAC CAGACAGAAG
    AGCAGCCCCC GGAAGCCCGA ACCTCTCAGC CCTGGGAAGT CAGCCAGGGA TGCCTCACCA
    AGAGACCTGA CAACCAAGCC TAGCCGGCCG TCCACCCCAG CCCTGGTCCT TGCCCCAGCT
    TACCTCCTGT CCTCCAGCCC CCAGCCCACC AGCAGCAGCT TCCCTTTCTT CCACCTGCTG
    GGCCCTACAC CTTTCCCGAT GCTGATGGGG CCTCCAGGCT CCAAGGGAGA CTGTGGCTTG
    CCGGGTCCCC CTGGGCTTCC AGGATTACCT GGATCTCCGG GCGCACGAGG TCCTCGGGGT
    CCTCCTGGGC CGTACGGGAA CCCAGGCCCA CCTGGCCCTC CAGGAGCCAA AGGACAGAAA
    GGGGACCCAG GACTCTCACC AGGACAGGCT CACGATGGAG CAAAGGGCAA CATGGGCTTG
    CCTGGGCTCT CCGGAAATCC AGGACCCTTG GGAAGGAAGG GACACAAGGG CCATCCTGGA
    GCAGCAGGGC ACCCTGGAGA ACAGGGGCAG CCAGGACCTG AGGGCAGTCC AGGGGCCAAA
    GGTTACCCTG GCAGGCAGGG GTTCCCTGGA CCTGTAGGAG ACCCTGGCCC CAAAGGCAGC
    AGGGGTTACA TTGGTCTTCC GGGGCTCTTT GGCCTGCCAG GGTCGGATGG AGAGCGGGGT
    CTCCCTGGCG TTCCTGGCAA GAGGGGCGAG ATGGGTAGGC CGGGCTTCCC TGGAGACTTT
    GGGGAAAGAG GCCCCCCTGG ACTTGATGGC AACCCTGGAG AAATTGGCCT GCCGGGGCCA
    CCAGGAGTGC TCGGGCTCAT TGGTGACACA GGAGCACTGG GCCCAGTTGG CTACCCAGGA
    CCGAAAGGCA TGAAGGGACT GATGGGCGGC GTGGGGGAGC CCGGACTGAA AGGTGATAAG
    GGTGAACAAG GGGTGCCAGG TGTGTCGGGA GATCCTGGCT TCCAAGGAGA CAAGGGGAGT
    CATGGCTTGC CAGGGCTCCC GGGTGGCCGG GGGAAGCCAG GGCCTCTGGG AAAAGCTGGA
    GACAAAGGAT CACTTGGGTT TCCTGGACCT CCTGGACCTG AGGGTTTCCC AGGAGACATT
    GGCCCTCCAG GGGACAATGG CCCTGAAGGC ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG
    CCTGGACCTC CTGGGCAGCT GGGGCCTGAG GGAGATGAAG GACCCATGGG GCCACCAGGA
    GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT
    GGCTCGAAGG GGGAACCAGG GGATCCTGGA CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT
    ATGGGATTCA TTGGTCTTGT CGGAGAGCCT GGAATTGTTG GGGAGAAGGG AGACCGTGGG
    GTGATGGGAC CCCCAGGTGC ACCTGGACCC AAGGGGTCGA TGGTAAGGAG GCCATCCTGG
    AACACCCGGT GGTATTGGGA ACCCTGGAGA GCCTGGACCC TGGGGCCCTC CAGGATCTCG
    AGGCCTGCCA GGCATGAGGG GAGCCAAGGG GCACCGGGGT CTCCAGCTGA CTTACACAGG
    CTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu74976
    Clone ID Related Accession (Same CDS sequence) XM_011250057.3 , XM_011250057.2
    Accession Version XM_011250057.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3708bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product collagen alpha-1(XXVII) chain isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011250057.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGGCACGG GATTCGCGCG GGGGGCCCGA GGCACAGCGG CGTCAGGACC CGGGGGGGGG 
    TTTCTCTTCG CCTGGATCTT GGTCTCATTT ACCTGTCACC TGGCCTCCAC CCAAGGAGCT
    CCTGAAGATG TGGATGTCCT CCAGCGGCTG GGCCTCAGCT GGACGAAGGC CGGGGGTGGC
    CGGAGTCCTA CACCCCCTGG TGTCATTCCT TTCCCATCTG GCTTCATCTT CACACAGCGG
    GCCAAGCTGC AGGCCCCCAC AGCCAATGTT CTTCCCACCA CCCTGGGGCG GGAGCTGGCA
    TTGGTGTTGA GCCTCTGTTC ACATCGTGTG AACCATGCCT TCCTCTTTGC CATCCGCAGC
    AGGAAACACA AGCTGCAACT GGGTCTGCAG TTCCTCCCCG GCAGGACGAT CATCCACCTG
    GGGCCTCGGC AATCAGTGGC CTTCGACTTG GATGTGCATG ATGGGCGCTG GCACCACCTG
    GCCCTAGAGT TGCGTGGCCG CACAGTCACA ATGGTGACAG CCTGTGGGCA GCACAGGGTA
    CCTGTCCCAC TGCCTTCCCG TAGGGACTCC ATGCTTGACC CCCAGGGCTC CTTTCTCTTG
    GGGAAGGTGA ACCCCCGTGC TGTCCAGTTT GAAGGTGCAC TCTGTCAGTT CAGCATCCAC
    CCTGTGGCGC AGGTCGCTCA CAATTACTGT GCCCACCTGA GAGAGCGGTG CCGACAGGTG
    GACACATATA GTCCCCAGGT GGGAACCCTC TTTCCTTGGG ACTCTGGCCC AGCCTTTGCT
    TTGCATCCTG AACCAGCCTT GCTTGGCTTG GGGAATCTGA CCAGAACTCC AGCAACCCTG
    GGGGCCAGGC CTGTAAGCAG GGCACTTGCA GTGACTCTGG CTCCTGCCAT GCCCACCAAG
    CCCCTGAGGA CTGTCCACCC AGATGTCTCT GAACACAGCT CTTCCCAGAC CCCACTGTCA
    CCTGCCAAAC AGTCGGCCAG GAAAACACCT TCCCCCTCCT CGTCAGCTTC CCTGGCTAAT
    TCCACCAGGG TTTATCGTCC AGCTGCTGCC CAGCCAAGAC AGATCACAAC TACTAGCCCC
    ACCAAACGTT CCCCCACCAA GCCTTCTGTT TCTCCCCTTT CAGTTACCCC CATGAAAAGT
    CCCCATGCAA CCCAGAAAAC AGGTGTCCCT TCATTCACAA AGCCTGTTCC ACCCACTCAG
    AAGCCAGCGC CCTTCACCTC CTACCTAGCA CCTTCCAAAG CCTCTAGTCC CACTGTGAGG
    CCTGTTCAGA AGACTTTCAT GACACCCCGA CCTCCAGTCC CCAGCCCCCA GCCCCTGCGC
    CCTACTACTG GCTTATCTAA GAAGTTCACT AACCCCACTG TTGCAAAATC TAAGTCCAAG
    ACGACCAGTT GGGCCAGCAA GCCGGTCTTG GCCCGCTCCA GTGTCCCTAA AACGCTTCAG
    CAAACTGTTT TGTCCCAGTC TCCTGTTTCC TATCTGGGCT CTCAGACACT GGCTCCAGCC
    CTCCCTCCTT TGGGTGTTGG AAATCCTAGA ACGATGCCTC CCACACGTGA CTCTGCTTTA
    ACTCCTGCTG GAAGCAAGAA ATTCACAGGA CGGGAAACCT CCAAGAAAAC CAGACAGAAG
    AGCAGCCCCC GGAAGCCCGA ACCTCTCAGC CCTGGGAAGT CAGCCAGGGA TGCCTCACCA
    AGAGACCTGA CAACCAAGCC TAGCCGGCCG TCCACCCCAG CCCTGGTCCT TGCCCCAGCT
    TACCTCCTGT CCTCCAGCCC CCAGCCCACC AGCAGCAGCT TCCCTTTCTT CCACCTGCTG
    GGCCCTACAC CTTTCCCGAT GCTGATGGGG CCTCCAGGCT CCAAGGGAGA CTGTGGCTTG
    CCGGGTCCCC CTGGGCTTCC AGGATTACCT GGATCTCCGG GCGCACGAGG TCCTCGGGGT
    CCTCCTGGGC CGTACGGGAA CCCAGGCCCA CCTGGCCCTC CAGGAGCCAA AGGACAGAAA
    GGGGACCCAG GACTCTCACC AGGACAGGCT CACGATGGAG CAAAGGGCAA CATGGGCTTG
    CCTGGGCTCT CCGGAAATCC AGGACCCTTG GGAAGGAAGG GACACAAGGG CCATCCTGGA
    GCAGCAGGGC ACCCTGGAGA ACAGGGGCAG CCAGGACCTG AGGGCAGTCC AGGGGCCAAA
    GGTTACCCTG GCAGGCAGGG GTTCCCTGGA CCTGTAGGAG ACCCTGGCCC CAAAGGCAGC
    AGGGGTTACA TTGGTCTTCC GGGGCTCTTT GGCCTGCCAG GGTCGGATGG AGAGCGGGGT
    CTCCCTGGCG TTCCTGGCAA GAGGGGCGAG ATGGGTAGGC CGGGCTTCCC TGGAGACTTT
    GGGGAAAGAG GCCCCCCTGG ACTTGATGGC AACCCTGGAG AAATTGGCCT GCCGGGGCCA
    CCAGGAGTGC TCGGGCTCAT TGGTGACACA GGAGCACTGG GCCCAGTTGG CTACCCAGGA
    CCGAAAGGCA TGAAGGGACT GATGGGCGGC GTGGGGGAGC CCGGACTGAA AGGTGATAAG
    GGTGAACAAG GGGTGCCAGG TGTGTCGGGA GATCCTGGCT TCCAAGGAGA CAAGGGGAGT
    CATGGCTTGC CAGGGCTCCC GGGTGGCCGG GGGAAGCCAG GGCCTCTGGG AAAAGCTGGA
    GACAAAGGAT CACTTGGGTT TCCTGGACCT CCTGGACCTG AGGGTTTCCC AGGAGACATT
    GGCCCTCCAG GGGACAATGG CCCTGAAGGC ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG
    CCTGGACCTC CTGGGCAGCT GGGGCCTGAG GGAGATGAAG GACCCATGGG GCCACCAGGA
    GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT
    GGCTCGAAGG GGGAACCAGG GGATCCTGGA CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT
    ATGGGATTCA TTGGTCTTGT CGGAGAGCCT GGAATTGTTG GGGAGAAGGG AGACCGTGGG
    GTGATGGGAC CCCCAGGTGC ACCTGGACCC AAGGGGTCGA TGGGCCATCC TGGAACACCC
    GGTGGTATTG GGAACCCTGG AGAGCCTGGA CCCTGGGGCC CTCCAGGATC TCGAGGCCTG
    CCAGGCATGA GGGGAGCCAA GGGGCACCGG GGCCCTCGAG GACCTGATGG ACCAGCTGGG
    GAGCAGGGAT CCAAGGGCCT GAAGGGTCGT GTAGGACCTC GGGGCAGACC TGGCCAGCCA
    GGACAGCAGG GTGCAGCTGG TGAGCGAGGC CACTCAGGTG CAAAAGGCTT CCTTGGCATC
    CCTGGTCCCT CAGGCCCTCC AGGTGCCAAG GGCCTCCCAG GAGAACCGGG CTCTCAGGGA
    CCTCAGGGGC CAGTTGGTCC TCCAGGAGAA ATGGGGCCCA AGGGACCGCC GGGTGCAGTG
    GGAGAGCCTG GCCTTCCTGG GGACTCCGGA ATGAAGGGTG ACCTCGGACC TCTGGGCCCC
    CCTGGGGAGC AAGGTCTTAT TGGGCAGCGG GGAGAGCCAG GCCTTGAGGG AGACCATGGC
    CCTGTGGGGC CAGATGGGCT GAAGGGCGAC AGGGGCGACC CAGGGCCCGA TGGCGAGCAT
    GGTGAAAAGG GCCAGGAAGG GCTGAAAGGA GAGGATGGGT CCCCTGGGCC CCCGGGCATC
    ACTGGCGTCC CGATCCATCC ACAGACACTC CAGCTCAGAT GGTCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011248359.1
    CDS508..4215
    Translation

    Target ORF information:

    RefSeq Version XM_011250057.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011250057.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGGCACGG GATTCGCGCG GGGGGCCCGA GGCACAGCGG CGTCAGGACC CGGGGGGGGG 
    TTTCTCTTCG CCTGGATCTT GGTCTCATTT ACCTGTCACC TGGCCTCCAC CCAAGGAGCT
    CCTGAAGATG TGGATGTCCT CCAGCGGCTG GGCCTCAGCT GGACGAAGGC CGGGGGTGGC
    CGGAGTCCTA CACCCCCTGG TGTCATTCCT TTCCCATCTG GCTTCATCTT CACACAGCGG
    GCCAAGCTGC AGGCCCCCAC AGCCAATGTT CTTCCCACCA CCCTGGGGCG GGAGCTGGCA
    TTGGTGTTGA GCCTCTGTTC ACATCGTGTG AACCATGCCT TCCTCTTTGC CATCCGCAGC
    AGGAAACACA AGCTGCAACT GGGTCTGCAG TTCCTCCCCG GCAGGACGAT CATCCACCTG
    GGGCCTCGGC AATCAGTGGC CTTCGACTTG GATGTGCATG ATGGGCGCTG GCACCACCTG
    GCCCTAGAGT TGCGTGGCCG CACAGTCACA ATGGTGACAG CCTGTGGGCA GCACAGGGTA
    CCTGTCCCAC TGCCTTCCCG TAGGGACTCC ATGCTTGACC CCCAGGGCTC CTTTCTCTTG
    GGGAAGGTGA ACCCCCGTGC TGTCCAGTTT GAAGGTGCAC TCTGTCAGTT CAGCATCCAC
    CCTGTGGCGC AGGTCGCTCA CAATTACTGT GCCCACCTGA GAGAGCGGTG CCGACAGGTG
    GACACATATA GTCCCCAGGT GGGAACCCTC TTTCCTTGGG ACTCTGGCCC AGCCTTTGCT
    TTGCATCCTG AACCAGCCTT GCTTGGCTTG GGGAATCTGA CCAGAACTCC AGCAACCCTG
    GGGGCCAGGC CTGTAAGCAG GGCACTTGCA GTGACTCTGG CTCCTGCCAT GCCCACCAAG
    CCCCTGAGGA CTGTCCACCC AGATGTCTCT GAACACAGCT CTTCCCAGAC CCCACTGTCA
    CCTGCCAAAC AGTCGGCCAG GAAAACACCT TCCCCCTCCT CGTCAGCTTC CCTGGCTAAT
    TCCACCAGGG TTTATCGTCC AGCTGCTGCC CAGCCAAGAC AGATCACAAC TACTAGCCCC
    ACCAAACGTT CCCCCACCAA GCCTTCTGTT TCTCCCCTTT CAGTTACCCC CATGAAAAGT
    CCCCATGCAA CCCAGAAAAC AGGTGTCCCT TCATTCACAA AGCCTGTTCC ACCCACTCAG
    AAGCCAGCGC CCTTCACCTC CTACCTAGCA CCTTCCAAAG CCTCTAGTCC CACTGTGAGG
    CCTGTTCAGA AGACTTTCAT GACACCCCGA CCTCCAGTCC CCAGCCCCCA GCCCCTGCGC
    CCTACTACTG GCTTATCTAA GAAGTTCACT AACCCCACTG TTGCAAAATC TAAGTCCAAG
    ACGACCAGTT GGGCCAGCAA GCCGGTCTTG GCCCGCTCCA GTGTCCCTAA AACGCTTCAG
    CAAACTGTTT TGTCCCAGTC TCCTGTTTCC TATCTGGGCT CTCAGACACT GGCTCCAGCC
    CTCCCTCCTT TGGGTGTTGG AAATCCTAGA ACGATGCCTC CCACACGTGA CTCTGCTTTA
    ACTCCTGCTG GAAGCAAGAA ATTCACAGGA CGGGAAACCT CCAAGAAAAC CAGACAGAAG
    AGCAGCCCCC GGAAGCCCGA ACCTCTCAGC CCTGGGAAGT CAGCCAGGGA TGCCTCACCA
    AGAGACCTGA CAACCAAGCC TAGCCGGCCG TCCACCCCAG CCCTGGTCCT TGCCCCAGCT
    TACCTCCTGT CCTCCAGCCC CCAGCCCACC AGCAGCAGCT TCCCTTTCTT CCACCTGCTG
    GGCCCTACAC CTTTCCCGAT GCTGATGGGG CCTCCAGGCT CCAAGGGAGA CTGTGGCTTG
    CCGGGTCCCC CTGGGCTTCC AGGATTACCT GGATCTCCGG GCGCACGAGG TCCTCGGGGT
    CCTCCTGGGC CGTACGGGAA CCCAGGCCCA CCTGGCCCTC CAGGAGCCAA AGGACAGAAA
    GGGGACCCAG GACTCTCACC AGGACAGGCT CACGATGGAG CAAAGGGCAA CATGGGCTTG
    CCTGGGCTCT CCGGAAATCC AGGACCCTTG GGAAGGAAGG GACACAAGGG CCATCCTGGA
    GCAGCAGGGC ACCCTGGAGA ACAGGGGCAG CCAGGACCTG AGGGCAGTCC AGGGGCCAAA
    GGTTACCCTG GCAGGCAGGG GTTCCCTGGA CCTGTAGGAG ACCCTGGCCC CAAAGGCAGC
    AGGGGTTACA TTGGTCTTCC GGGGCTCTTT GGCCTGCCAG GGTCGGATGG AGAGCGGGGT
    CTCCCTGGCG TTCCTGGCAA GAGGGGCGAG ATGGGTAGGC CGGGCTTCCC TGGAGACTTT
    GGGGAAAGAG GCCCCCCTGG ACTTGATGGC AACCCTGGAG AAATTGGCCT GCCGGGGCCA
    CCAGGAGTGC TCGGGCTCAT TGGTGACACA GGAGCACTGG GCCCAGTTGG CTACCCAGGA
    CCGAAAGGCA TGAAGGGACT GATGGGCGGC GTGGGGGAGC CCGGACTGAA AGGTGATAAG
    GGTGAACAAG GGGTGCCAGG TGTGTCGGGA GATCCTGGCT TCCAAGGAGA CAAGGGGAGT
    CATGGCTTGC CAGGGCTCCC GGGTGGCCGG GGGAAGCCAG GGCCTCTGGG AAAAGCTGGA
    GACAAAGGAT CACTTGGGTT TCCTGGACCT CCTGGACCTG AGGGTTTCCC AGGAGACATT
    GGCCCTCCAG GGGACAATGG CCCTGAAGGC ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG
    CCTGGACCTC CTGGGCAGCT GGGGCCTGAG GGAGATGAAG GACCCATGGG GCCACCAGGA
    GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT
    GGCTCGAAGG GGGAACCAGG GGATCCTGGA CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT
    ATGGGATTCA TTGGTCTTGT CGGAGAGCCT GGAATTGTTG GGGAGAAGGG AGACCGTGGG
    GTGATGGGAC CCCCAGGTGC ACCTGGACCC AAGGGGTCGA TGGGCCATCC TGGAACACCC
    GGTGGTATTG GGAACCCTGG AGAGCCTGGA CCCTGGGGCC CTCCAGGATC TCGAGGCCTG
    CCAGGCATGA GGGGAGCCAA GGGGCACCGG GGCCCTCGAG GACCTGATGG ACCAGCTGGG
    GAGCAGGGAT CCAAGGGCCT GAAGGGTCGT GTAGGACCTC GGGGCAGACC TGGCCAGCCA
    GGACAGCAGG GTGCAGCTGG TGAGCGAGGC CACTCAGGTG CAAAAGGCTT CCTTGGCATC
    CCTGGTCCCT CAGGCCCTCC AGGTGCCAAG GGCCTCCCAG GAGAACCGGG CTCTCAGGGA
    CCTCAGGGGC CAGTTGGTCC TCCAGGAGAA ATGGGGCCCA AGGGACCGCC GGGTGCAGTG
    GGAGAGCCTG GCCTTCCTGG GGACTCCGGA ATGAAGGGTG ACCTCGGACC TCTGGGCCCC
    CCTGGGGAGC AAGGTCTTAT TGGGCAGCGG GGAGAGCCAG GCCTTGAGGG AGACCATGGC
    CCTGTGGGGC CAGATGGGCT GAAGGGCGAC AGGGGCGACC CAGGGCCCGA TGGCGAGCAT
    GGTGAAAAGG GCCAGGAAGG GCTGAAAGGA GAGGATGGGT CCCCTGGGCC CCCGGGCATC
    ACTGGCGTCC CGATCCATCC ACAGACACTC CAGCTCAGAT GGTCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu74977
    Clone ID Related Accession (Same CDS sequence) XM_011250058.3 , XM_011250058.2
    Accession Version XM_011250058.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3186bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product collagen alpha-1(XXVII) chain isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011250058.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGGCACGG GATTCGCGCG GGGGGCCCGA GGCACAGCGG CGTCAGGACC CGGGGGGGGG 
    TTTCTCTTCG CCTGGATCTT GGTCTCATTT ACCTGTCACC TGGCCTCCAC CCAAGGAGCT
    CCTGAAGATG TGGATGTCCT CCAGCGGCTG GGCCTCAGCT GGACGAAGGC CGGGGGTGGC
    CGGAGTCCTA CACCCCCTGG TGTCATTCCT TTCCCATCTG GCTTCATCTT CACACAGCGG
    GCCAAGCTGC AGGCCCCCAC AGCCAATGTT CTTCCCACCA CCCTGGGGCG GGAGCTGGCA
    TTGGTGTTGA GCCTCTGTTC ACATCGTGTG AACCATGCCT TCCTCTTTGC CATCCGCAGC
    AGGAAACACA AGCTGCAACT GGGTCTGCAG TTCCTCCCCG GCAGGACGAT CATCCACCTG
    GGGCCTCGGC AATCAGTGGC CTTCGACTTG GATGTGCATG ATGGGCGCTG GCACCACCTG
    GCCCTAGAGT TGCGTGGCCG CACAGTCACA ATGGTGACAG CCTGTGGGCA GCACAGGGTA
    CCTGTCCCAC TGCCTTCCCG TAGGGACTCC ATGCTTGACC CCCAGGGCTC CTTTCTCTTG
    GGGAAGGTGA ACCCCCGTGC TGTCCAGTTT GAAGGTGCAC TCTGTCAGTT CAGCATCCAC
    CCTGTGGCGC AGGTCGCTCA CAATTACTGT GCCCACCTGA GAGAGCGGTG CCGACAGGTG
    GACACATATA GTCCCCAGGT GGGAACCCTC TTTCCTTGGG ACTCTGGCCC AGCCTTTGCT
    TTGCATCCTG AACCAGCCTT GCTTGGCTTG GGGAATCTGA CCAGAACTCC AGCAACCCTG
    GGGGCCAGGC CTGTAAGCAG GGCACTTGCA GTGACTCTGG CTCCTGCCAT GCCCACCAAG
    CCCCTGAGGA CTGTCCACCC AGATGTCTCT GAACACAGCT CTTCCCAGAC CCCACTGTCA
    CCTGCCAAAC AGTCGGCCAG GAAAACACCT TCCCCCTCCT CGTCAGCTTC CCTGGCTAAT
    TCCACCAGGG TTTATCGTCC AGCTGCTGCC CAGCCAAGAC AGATCACAAC TACTAGCCCC
    ACCAAACGTT CCCCCACCAA GCCTTCTGTT TCTCCCCTTT CAGTTACCCC CATGAAAAGT
    CCCCATGCAA CCCAGAAAAC AGGTGTCCCT TCATTCACAA AGCCTGTTCC ACCCACTCAG
    AAGCCAGCGC CCTTCACCTC CTACCTAGCA CCTTCCAAAG CCTCTAGTCC CACTGTGAGG
    CCTGTTCAGA AGACTTTCAT GACACCCCGA CCTCCAGTCC CCAGCCCCCA GCCCCTGCGC
    CCTACTACTG GCTTATCTAA GAAGTTCACT AACCCCACTG TTGCAAAATC TAAGTCCAAG
    ACGACCAGTT GGGCCAGCAA GCCGGTCTTG GCCCGCTCCA GTGTCCCTAA AACGCTTCAG
    CAAACTGTTT TGTCCCAGTC TCCTGTTTCC TATCTGGGCT CTCAGACACT GGCTCCAGCC
    CTCCCTCCTT TGGGTGTTGG AAATCCTAGA ACGATGCCTC CCACACGTGA CTCTGCTTTA
    ACTCCTGCTG GAAGCAAGAA ATTCACAGGA CGGGAAACCT CCAAGAAAAC CAGACAGAAG
    AGCAGCCCCC GGAAGCCCGA ACCTCTCAGC CCTGGGAAGT CAGCCAGGGA TGCCTCACCA
    AGAGACCTGA CAACCAAGCC TAGCCGGCCG TCCACCCCAG CCCTGGTCCT TGCCCCAGCT
    TACCTCCTGT CCTCCAGCCC CCAGCCCACC AGCAGCAGCT TCCCTTTCTT CCACCTGCTG
    GGCCCTACAC CTTTCCCGAT GCTGATGGGG CCTCCAGGCT CCAAGGGAGA CTGTGGCTTG
    CCGGGTCCCC CTGGGCTTCC AGGATTACCT GGATCTCCGG GCGCACGAGG TCCTCGGGGT
    CCTCCTGGGC CGTACGGGAA CCCAGGCCCA CCTGGCCCTC CAGGAGCCAA AGGACAGAAA
    GGGGACCCAG GACTCTCACC AGGACAGGCT CACGATGGAG CAAAGGGCAA CATGGGCTTG
    CCTGGGCTCT CCGGAAATCC AGGACCCTTG GGAAGGAAGG GACACAAGGG CCATCCTGGA
    GCAGCAGGGC ACCCTGGAGA ACAGGGGCAG CCAGGACCTG AGGGCAGTCC AGGGGCCAAA
    GGTTACCCTG GCAGGCAGGG GTTCCCTGGA CCTGTAGGAG ACCCTGGCCC CAAAGGCAGC
    AGGGGTTACA TTGGTCTTCC GGGGCTCTTT GGCCTGCCAG GGTCGGATGG AGAGCGGGGT
    CTCCCTGGCG TTCCTGGCAA GAGGGGCGAG ATGGGTAGGC CGGGCTTCCC TGGAGACTTT
    GGGGAAAGAG GCCCCCCTGG ACTTGATGGC AACCCTGGAG AAATTGGCCT GCCGGGGCCA
    CCAGGAGTGC TCGGGCTCAT TGGTGACACA GGAGCACTGG GCCCAGTTGG CTACCCAGGA
    CCGAAAGGCA TGAAGGGACT GATGGGCGGC GTGGGGGAGC CCGGACTGAA AGGTGATAAG
    GGTGAACAAG GGGTGCCAGG TGTGTCGGGA GATCCTGGCT TCCAAGGAGA CAAGGGGAGT
    CATGGCTTGC CAGGGCTCCC GGGTGGCCGG GGGAAGCCAG GGCCTCTGGG AAAAGCTGGA
    GACAAAGGAT CACTTGGGTT TCCTGGACCT CCTGGACCTG AGGGTTTCCC AGGAGACATT
    GGCCCTCCAG GGGACAATGG CCCTGAAGGC ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG
    CCTGGACCTC CTGGGCAGCT GGGGCCTGAG GGAGATGAAG GACCCATGGG GCCACCAGGA
    GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT
    GGCTCGAAGG GGGAACCAGG GGATCCTGGA CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT
    ATGGGATTCA TTGGTCTTGT CGGAGAGCCT GGAATTGTTG GGGAGAAGGG AGACCGTGGG
    GTGATGGGAC CCCCAGGTGC ACCTGGACCC AAGGGGTCGA TGGGCCATCC TGGAACACCC
    GGTGGTATTG GGAACCCTGG AGAGCCTGGA CCCTGGGGCC CTCCAGGATC TCGAGGCCTG
    CCAGGCATGA GGGGAGCCAA GGGGCACCGG GGTCTCCAGC TGACTTACAC AGGCTGTGAC
    CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011248360.1
    CDS507..3692
    Translation

    Target ORF information:

    RefSeq Version XM_011250058.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011250058.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGGCACGG GATTCGCGCG GGGGGCCCGA GGCACAGCGG CGTCAGGACC CGGGGGGGGG 
    TTTCTCTTCG CCTGGATCTT GGTCTCATTT ACCTGTCACC TGGCCTCCAC CCAAGGAGCT
    CCTGAAGATG TGGATGTCCT CCAGCGGCTG GGCCTCAGCT GGACGAAGGC CGGGGGTGGC
    CGGAGTCCTA CACCCCCTGG TGTCATTCCT TTCCCATCTG GCTTCATCTT CACACAGCGG
    GCCAAGCTGC AGGCCCCCAC AGCCAATGTT CTTCCCACCA CCCTGGGGCG GGAGCTGGCA
    TTGGTGTTGA GCCTCTGTTC ACATCGTGTG AACCATGCCT TCCTCTTTGC CATCCGCAGC
    AGGAAACACA AGCTGCAACT GGGTCTGCAG TTCCTCCCCG GCAGGACGAT CATCCACCTG
    GGGCCTCGGC AATCAGTGGC CTTCGACTTG GATGTGCATG ATGGGCGCTG GCACCACCTG
    GCCCTAGAGT TGCGTGGCCG CACAGTCACA ATGGTGACAG CCTGTGGGCA GCACAGGGTA
    CCTGTCCCAC TGCCTTCCCG TAGGGACTCC ATGCTTGACC CCCAGGGCTC CTTTCTCTTG
    GGGAAGGTGA ACCCCCGTGC TGTCCAGTTT GAAGGTGCAC TCTGTCAGTT CAGCATCCAC
    CCTGTGGCGC AGGTCGCTCA CAATTACTGT GCCCACCTGA GAGAGCGGTG CCGACAGGTG
    GACACATATA GTCCCCAGGT GGGAACCCTC TTTCCTTGGG ACTCTGGCCC AGCCTTTGCT
    TTGCATCCTG AACCAGCCTT GCTTGGCTTG GGGAATCTGA CCAGAACTCC AGCAACCCTG
    GGGGCCAGGC CTGTAAGCAG GGCACTTGCA GTGACTCTGG CTCCTGCCAT GCCCACCAAG
    CCCCTGAGGA CTGTCCACCC AGATGTCTCT GAACACAGCT CTTCCCAGAC CCCACTGTCA
    CCTGCCAAAC AGTCGGCCAG GAAAACACCT TCCCCCTCCT CGTCAGCTTC CCTGGCTAAT
    TCCACCAGGG TTTATCGTCC AGCTGCTGCC CAGCCAAGAC AGATCACAAC TACTAGCCCC
    ACCAAACGTT CCCCCACCAA GCCTTCTGTT TCTCCCCTTT CAGTTACCCC CATGAAAAGT
    CCCCATGCAA CCCAGAAAAC AGGTGTCCCT TCATTCACAA AGCCTGTTCC ACCCACTCAG
    AAGCCAGCGC CCTTCACCTC CTACCTAGCA CCTTCCAAAG CCTCTAGTCC CACTGTGAGG
    CCTGTTCAGA AGACTTTCAT GACACCCCGA CCTCCAGTCC CCAGCCCCCA GCCCCTGCGC
    CCTACTACTG GCTTATCTAA GAAGTTCACT AACCCCACTG TTGCAAAATC TAAGTCCAAG
    ACGACCAGTT GGGCCAGCAA GCCGGTCTTG GCCCGCTCCA GTGTCCCTAA AACGCTTCAG
    CAAACTGTTT TGTCCCAGTC TCCTGTTTCC TATCTGGGCT CTCAGACACT GGCTCCAGCC
    CTCCCTCCTT TGGGTGTTGG AAATCCTAGA ACGATGCCTC CCACACGTGA CTCTGCTTTA
    ACTCCTGCTG GAAGCAAGAA ATTCACAGGA CGGGAAACCT CCAAGAAAAC CAGACAGAAG
    AGCAGCCCCC GGAAGCCCGA ACCTCTCAGC CCTGGGAAGT CAGCCAGGGA TGCCTCACCA
    AGAGACCTGA CAACCAAGCC TAGCCGGCCG TCCACCCCAG CCCTGGTCCT TGCCCCAGCT
    TACCTCCTGT CCTCCAGCCC CCAGCCCACC AGCAGCAGCT TCCCTTTCTT CCACCTGCTG
    GGCCCTACAC CTTTCCCGAT GCTGATGGGG CCTCCAGGCT CCAAGGGAGA CTGTGGCTTG
    CCGGGTCCCC CTGGGCTTCC AGGATTACCT GGATCTCCGG GCGCACGAGG TCCTCGGGGT
    CCTCCTGGGC CGTACGGGAA CCCAGGCCCA CCTGGCCCTC CAGGAGCCAA AGGACAGAAA
    GGGGACCCAG GACTCTCACC AGGACAGGCT CACGATGGAG CAAAGGGCAA CATGGGCTTG
    CCTGGGCTCT CCGGAAATCC AGGACCCTTG GGAAGGAAGG GACACAAGGG CCATCCTGGA
    GCAGCAGGGC ACCCTGGAGA ACAGGGGCAG CCAGGACCTG AGGGCAGTCC AGGGGCCAAA
    GGTTACCCTG GCAGGCAGGG GTTCCCTGGA CCTGTAGGAG ACCCTGGCCC CAAAGGCAGC
    AGGGGTTACA TTGGTCTTCC GGGGCTCTTT GGCCTGCCAG GGTCGGATGG AGAGCGGGGT
    CTCCCTGGCG TTCCTGGCAA GAGGGGCGAG ATGGGTAGGC CGGGCTTCCC TGGAGACTTT
    GGGGAAAGAG GCCCCCCTGG ACTTGATGGC AACCCTGGAG AAATTGGCCT GCCGGGGCCA
    CCAGGAGTGC TCGGGCTCAT TGGTGACACA GGAGCACTGG GCCCAGTTGG CTACCCAGGA
    CCGAAAGGCA TGAAGGGACT GATGGGCGGC GTGGGGGAGC CCGGACTGAA AGGTGATAAG
    GGTGAACAAG GGGTGCCAGG TGTGTCGGGA GATCCTGGCT TCCAAGGAGA CAAGGGGAGT
    CATGGCTTGC CAGGGCTCCC GGGTGGCCGG GGGAAGCCAG GGCCTCTGGG AAAAGCTGGA
    GACAAAGGAT CACTTGGGTT TCCTGGACCT CCTGGACCTG AGGGTTTCCC AGGAGACATT
    GGCCCTCCAG GGGACAATGG CCCTGAAGGC ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG
    CCTGGACCTC CTGGGCAGCT GGGGCCTGAG GGAGATGAAG GACCCATGGG GCCACCAGGA
    GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT
    GGCTCGAAGG GGGAACCAGG GGATCCTGGA CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT
    ATGGGATTCA TTGGTCTTGT CGGAGAGCCT GGAATTGTTG GGGAGAAGGG AGACCGTGGG
    GTGATGGGAC CCCCAGGTGC ACCTGGACCC AAGGGGTCGA TGGGCCATCC TGGAACACCC
    GGTGGTATTG GGAACCCTGG AGAGCCTGGA CCCTGGGGCC CTCCAGGATC TCGAGGCCTG
    CCAGGCATGA GGGGAGCCAA GGGGCACCGG GGTCTCCAGC TGACTTACAC AGGCTGTGAC
    CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu81039
    Clone ID Related Accession (Same CDS sequence) XM_017320285.1
    Accession Version XM_017320285.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3342bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product collagen alpha-1(XXVII) chain isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGAGAGCG GGGTCTCCCT GGCGTTCCTG GCAAGAGGGG CGAGATGGGG CTTCCCTGGA 
    GACTTTGGGG AAAGAGGCCC CCCTGGACTT GATGGCAACC CTGGAGAAAT TGGCCTGCCG
    GGGCCACCAG GAGTGCTCGG GCTCATTGGT GACACAGGAG CACTGGGCCC AGTTGGCTAC
    CCAGGACCGA AAGGCATGAA GGGACTGATG GGCGGCGTGG GGGAGCCCGG ACTGAAAGGT
    GATAAGGGTG AACAAGGGGT GCCAGGTGTG TCGGGAGATC CTGGCTTCCA AGGAGACAAG
    GGGAGTCATG GCTTGCCAGG GCTCCCGGGT GGCCGGGGGA AGCCAGGGCC TCTGGGAAAA
    GCTGGAGACA AAGGATCACT TGGGTTTCCT GGACCTCCTG GACCTGAGGG TTTCCCAGGA
    GACATTGGCC CTCCAGGGGA CAATGGCCCT GAAGGCATGA AGGGTAAGCC TGGAGCCCGA
    GGCCTGCCTG GACCTCCTGG GCAGCTGGGG CCTGAGGGAG ATGAAGGACC CATGGGGCCA
    CCAGGAGTCC CAGGCTTGGA GGGTCAGCCT GGAAGGAAAG GGTTTCCTGG GAGGCCTGGC
    CTGGATGGCT CGAAGGGGGA ACCAGGGGAT CCTGGACGGC CAGGACCTGT GGGTGAACAG
    GGGCTTATGG GATTCATTGG TCTTGTCGGA GAGCCTGGAA TTGTTGGGGA GAAGGGAGAC
    CGTGGGGTGA TGGGACCCCC AGGTGCACCT GGACCCAAGG GGTCGATGGG CCATCCTGGA
    ACACCCGGTG GTATTGGGAA CCCTGGAGAG CCTGGACCCT GGGGCCCTCC AGGATCTCGA
    GGCCTGCCAG GCATGAGGGG AGCCAAGGGG CACCGGGGCC CTCGAGGACC TGATGGACCA
    GCTGGGGAGC AGGGATCCAA GGGCCTGAAG GGTCGTGTAG GACCTCGGGG CAGACCTGGC
    CAGCCAGGAC AGCAGGGTGC AGCTGGTGAG CGAGGCCACT CAGGTGCAAA AGGCTTCCTT
    GGCATCCCTG GTCCCTCAGG CCCTCCAGGT GCCAAGGGCC TCCCAGGAGA ACCGGGCTCT
    CAGGGACCTC AGGGGCCAGT TGGTCCTCCA GGAGAAATGG GGCCCAAGGG ACCGCCGGGT
    GCAGTGGGAG AGCCTGGCCT TCCTGGGGAC TCCGGAATGA AGGGTGACCT CGGACCTCTG
    GGCCCCCCTG GGGAGCAAGG TCTTATTGGG CAGCGGGGAG AGCCAGGCCT TGAGGGAGAC
    CATGGCCCTG TGGGGCCAGA TGGGCTGAAG GGCGACAGGG GCGACCCAGG GCCCGATGGC
    GAGCATGGTG AAAAGGGCCA GGAAGGGCTG AAAGGAGAGG ATGGGTCCCC TGGGCCCCCG
    GGCATCACTG GCGTCCCGGG TCGTGAGGGG AAGCCGGGCA AGCAGGGTGA GAAAGGCCAG
    AGGGGAGCTA AGGGTGCCAA GGGCCACCAA GGATATCTGG GAGAGATGGG CATCCCCGGA
    GAACCTGGGC CCCCTGGGAC CCCAGGTCCT AAAGGGTCCC GGGGCACCCT AGGACCAACG
    GGTGCTCCAG GACGGATGGG AGCCCAAGGA GAACCAGGAT TGGCCGGTTA TAATGGACAC
    AAAGGCATCA CAGGACCCCT CGGGCCTCCC GGGCCCAAAG GCGAGAAGGG GGACCAGGGC
    GAGGATGGCA AGACCGAGGG GCCCCCTGGG CCACCAGGAG ATCGGGGTCC TGTGGGTGAT
    CGAGGAGACC GTGGGGAGCC AGGCGACCCT GGATACCCTG GTCAAGAGGG AGTTCAAGGC
    CTCCGTGGAG AACCGGGCCA GCAGGGACAG CCTGGGCATC CAGGACCCCG GGGGCGCCCA
    GGACCCAAAG GATCAAAAGG CGAAGAGGGC CCAAAGGGAA AGCCAGGCAA GGCTGGGCCA
    TCAGGCCGGA GGGGGACCCA GGTGAGTTGC CATAGCTTCT CTGTCACCTT CTATGTTCTC
    CTGAGAAGGT TTAACCAGCT TCTTCTGTAC CTCCAGGGCC TTCAAGGACT GCCAGGCCCC
    CGAGGCGTGG TGGGGAGACA GGGCCCTGAG GGCACTGCTG GATCTGATGG GATTCCTGGC
    AGAGATGGTC GGCCAGGATA TCAGGGAGAC CAGGGAAATG ATGGGGACCC TGGCCCTGTG
    GGCCCTGCTG GGAGAAGAGG AAATCCAGGT GTGGCTGGCT TGCCTGGAGC ACAGGGGCCT
    CCGGGATTCA AGGGTGAAAG TGGGTTACCT GGGCAACTGG GTCCCCCTGG AAAACGAGGG
    ACAGAAGGTG GAACAGGGCT TCCTGGGAAC CAGGGGGAGC CAGGATCCAA AGGCCAGCCG
    GGTGACTCTG GCGAGATGGG CTTCCCAGGA GTGGCTGGCC TCTTTGGACC CAAGGGCCCC
    CCTGGAGACA TTGGCTTCAA AGGCATCCAA GGTCCTCGGG GGCCTCCTGG CTTGATGGGA
    AAGGAAGGTA TCATTGGCCC CCCCGGAATG CTGGGACCTT CTGGACTCCC GGGTCCCAAA
    GGTGACAGAG GCAGCCGAGG AGACTTGGGA CTGCAAGGCC CAAGGGGTCC TCCTGGTCCA
    AGGGGCCGGC CAGGTCCCCC GGGCCCTCCT TGGCATCCCA TCCAGTTTCA GCAAGATGAC
    CTTGGAGCAG CTTTCCAGAC ATGGATGGAT GCTCAAGGAG CCGTCAGATC GGAGCAGGGG
    TACAGCTATC CGGACCAGCT GGCGCTAGAC CAGGGAGGGG AGATCTTCAA AACCTTACAC
    TACCTCAGCA ACCTCATCCA GAGCATTAAG ACACCCTTGG GCACCAAGGA GAACCCAGCC
    CGGGTCTGCC GGGACCTCAT GGACTGTGAG CAGAGGATGG CGGATGGTAC CTACTGGGTG
    GACCCCAACC TCGGCTGCTC CTCTGACACC ATTGAAGTCT CCTGCAACTT TACACAGGGT
    GGGCAGACGT GCCTGAAGCC CATCACGGCC TCCAAGGCCG AGTTTGCTGT GAGTCGGGTC
    CAGATGAATT TCTTGCACCT GCTGAGCTCT GAGGGGACAC AGCATATCAC AATCCACTGT
    CTGAACATGA CGGTGTGGCA GGAGGGACCG GGACGCTCCT CTGCCAGACA GGCTGTGCGC
    TTCCGTGCCT GGAACGGACA GGTCTTCGAA GCTGGGGGTC AGTTCAGGCC AGAGGTGTCT
    ATGGATGGCT GCAAGGTCCA TGATGGCCGC TGGCATCAGA CACTGTTCAC CTTCCGGACC
    CAGGACCCCC AGCAGCTGCC CATCGTCAGT GTGGACAATC TCCCGCCTGT CTCATCAGGG
    AAGCAGTACC GCCTGGAAGT TGGACCTGCG TGCTTCCTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017175774.1
    CDS90..3431
    Translation

    Target ORF information:

    RefSeq Version XM_017320285.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017320285.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGAGAGCG GGGTCTCCCT GGCGTTCCTG GCAAGAGGGG CGAGATGGGG CTTCCCTGGA 
    GACTTTGGGG AAAGAGGCCC CCCTGGACTT GATGGCAACC CTGGAGAAAT TGGCCTGCCG
    GGGCCACCAG GAGTGCTCGG GCTCATTGGT GACACAGGAG CACTGGGCCC AGTTGGCTAC
    CCAGGACCGA AAGGCATGAA GGGACTGATG GGCGGCGTGG GGGAGCCCGG ACTGAAAGGT
    GATAAGGGTG AACAAGGGGT GCCAGGTGTG TCGGGAGATC CTGGCTTCCA AGGAGACAAG
    GGGAGTCATG GCTTGCCAGG GCTCCCGGGT GGCCGGGGGA AGCCAGGGCC TCTGGGAAAA
    GCTGGAGACA AAGGATCACT TGGGTTTCCT GGACCTCCTG GACCTGAGGG TTTCCCAGGA
    GACATTGGCC CTCCAGGGGA CAATGGCCCT GAAGGCATGA AGGGTAAGCC TGGAGCCCGA
    GGCCTGCCTG GACCTCCTGG GCAGCTGGGG CCTGAGGGAG ATGAAGGACC CATGGGGCCA
    CCAGGAGTCC CAGGCTTGGA GGGTCAGCCT GGAAGGAAAG GGTTTCCTGG GAGGCCTGGC
    CTGGATGGCT CGAAGGGGGA ACCAGGGGAT CCTGGACGGC CAGGACCTGT GGGTGAACAG
    GGGCTTATGG GATTCATTGG TCTTGTCGGA GAGCCTGGAA TTGTTGGGGA GAAGGGAGAC
    CGTGGGGTGA TGGGACCCCC AGGTGCACCT GGACCCAAGG GGTCGATGGG CCATCCTGGA
    ACACCCGGTG GTATTGGGAA CCCTGGAGAG CCTGGACCCT GGGGCCCTCC AGGATCTCGA
    GGCCTGCCAG GCATGAGGGG AGCCAAGGGG CACCGGGGCC CTCGAGGACC TGATGGACCA
    GCTGGGGAGC AGGGATCCAA GGGCCTGAAG GGTCGTGTAG GACCTCGGGG CAGACCTGGC
    CAGCCAGGAC AGCAGGGTGC AGCTGGTGAG CGAGGCCACT CAGGTGCAAA AGGCTTCCTT
    GGCATCCCTG GTCCCTCAGG CCCTCCAGGT GCCAAGGGCC TCCCAGGAGA ACCGGGCTCT
    CAGGGACCTC AGGGGCCAGT TGGTCCTCCA GGAGAAATGG GGCCCAAGGG ACCGCCGGGT
    GCAGTGGGAG AGCCTGGCCT TCCTGGGGAC TCCGGAATGA AGGGTGACCT CGGACCTCTG
    GGCCCCCCTG GGGAGCAAGG TCTTATTGGG CAGCGGGGAG AGCCAGGCCT TGAGGGAGAC
    CATGGCCCTG TGGGGCCAGA TGGGCTGAAG GGCGACAGGG GCGACCCAGG GCCCGATGGC
    GAGCATGGTG AAAAGGGCCA GGAAGGGCTG AAAGGAGAGG ATGGGTCCCC TGGGCCCCCG
    GGCATCACTG GCGTCCCGGG TCGTGAGGGG AAGCCGGGCA AGCAGGGTGA GAAAGGCCAG
    AGGGGAGCTA AGGGTGCCAA GGGCCACCAA GGATATCTGG GAGAGATGGG CATCCCCGGA
    GAACCTGGGC CCCCTGGGAC CCCAGGTCCT AAAGGGTCCC GGGGCACCCT AGGACCAACG
    GGTGCTCCAG GACGGATGGG AGCCCAAGGA GAACCAGGAT TGGCCGGTTA TAATGGACAC
    AAAGGCATCA CAGGACCCCT CGGGCCTCCC GGGCCCAAAG GCGAGAAGGG GGACCAGGGC
    GAGGATGGCA AGACCGAGGG GCCCCCTGGG CCACCAGGAG ATCGGGGTCC TGTGGGTGAT
    CGAGGAGACC GTGGGGAGCC AGGCGACCCT GGATACCCTG GTCAAGAGGG AGTTCAAGGC
    CTCCGTGGAG AACCGGGCCA GCAGGGACAG CCTGGGCATC CAGGACCCCG GGGGCGCCCA
    GGACCCAAAG GATCAAAAGG CGAAGAGGGC CCAAAGGGAA AGCCAGGCAA GGCTGGGCCA
    TCAGGCCGGA GGGGGACCCA GGTGAGTTGC CATAGCTTCT CTGTCACCTT CTATGTTCTC
    CTGAGAAGGT TTAACCAGCT TCTTCTGTAC CTCCAGGGCC TTCAAGGACT GCCAGGCCCC
    CGAGGCGTGG TGGGGAGACA GGGCCCTGAG GGCACTGCTG GATCTGATGG GATTCCTGGC
    AGAGATGGTC GGCCAGGATA TCAGGGAGAC CAGGGAAATG ATGGGGACCC TGGCCCTGTG
    GGCCCTGCTG GGAGAAGAGG AAATCCAGGT GTGGCTGGCT TGCCTGGAGC ACAGGGGCCT
    CCGGGATTCA AGGGTGAAAG TGGGTTACCT GGGCAACTGG GTCCCCCTGG AAAACGAGGG
    ACAGAAGGTG GAACAGGGCT TCCTGGGAAC CAGGGGGAGC CAGGATCCAA AGGCCAGCCG
    GGTGACTCTG GCGAGATGGG CTTCCCAGGA GTGGCTGGCC TCTTTGGACC CAAGGGCCCC
    CCTGGAGACA TTGGCTTCAA AGGCATCCAA GGTCCTCGGG GGCCTCCTGG CTTGATGGGA
    AAGGAAGGTA TCATTGGCCC CCCCGGAATG CTGGGACCTT CTGGACTCCC GGGTCCCAAA
    GGTGACAGAG GCAGCCGAGG AGACTTGGGA CTGCAAGGCC CAAGGGGTCC TCCTGGTCCA
    AGGGGCCGGC CAGGTCCCCC GGGCCCTCCT TGGCATCCCA TCCAGTTTCA GCAAGATGAC
    CTTGGAGCAG CTTTCCAGAC ATGGATGGAT GCTCAAGGAG CCGTCAGATC GGAGCAGGGG
    TACAGCTATC CGGACCAGCT GGCGCTAGAC CAGGGAGGGG AGATCTTCAA AACCTTACAC
    TACCTCAGCA ACCTCATCCA GAGCATTAAG ACACCCTTGG GCACCAAGGA GAACCCAGCC
    CGGGTCTGCC GGGACCTCAT GGACTGTGAG CAGAGGATGG CGGATGGTAC CTACTGGGTG
    GACCCCAACC TCGGCTGCTC CTCTGACACC ATTGAAGTCT CCTGCAACTT TACACAGGGT
    GGGCAGACGT GCCTGAAGCC CATCACGGCC TCCAAGGCCG AGTTTGCTGT GAGTCGGGTC
    CAGATGAATT TCTTGCACCT GCTGAGCTCT GAGGGGACAC AGCATATCAC AATCCACTGT
    CTGAACATGA CGGTGTGGCA GGAGGGACCG GGACGCTCCT CTGCCAGACA GGCTGTGCGC
    TTCCGTGCCT GGAACGGACA GGTCTTCGAA GCTGGGGGTC AGTTCAGGCC AGAGGTGTCT
    ATGGATGGCT GCAAGGTCCA TGATGGCCGC TGGCATCAGA CACTGTTCAC CTTCCGGACC
    CAGGACCCCC AGCAGCTGCC CATCGTCAGT GTGGACAATC TCCCGCCTGT CTCATCAGGG
    AAGCAGTACC GCCTGGAAGT TGGACCTGCG TGCTTCCTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu88567
    Clone ID Related Accession (Same CDS sequence) XM_030253632.1
    Accession Version XM_030253632.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3267bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product collagen alpha-1(XXVII) chain isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGAGAGCG GGGTCTCCCT GGCGTTCCTG GCAAGAGGGG CGAGATGGGG CTTCCCTGGA 
    GACTTTGGGG AAAGAGGCCC CCCTGGACTT GATGGCAACC CTGGAGAAAT TGGCCTGCCG
    GGGCCACCAG GAGTGCTCGG GCTCATTGGT GACACAGGAG CACTGGGCCC AGTTGGCTAC
    CCAGGACCGA AAGGCATGAA GGGACTGATG GGCGGCGTGG GGGAGCCCGG ACTGAAAGGT
    GATAAGGGTG AACAAGGGGT GCCAGGTGTG TCGGGAGATC CTGGCTTCCA AGGAGACAAG
    GGGAGTCATG GCTTGCCAGG GCTCCCGGGT GGCCGGGGGA AGCCAGGGCC TCTGGGAAAA
    GCTGGAGACA AAGGATCACT TGGGTTTCCT GGACCTCCTG GACCTGAGGG TTTCCCAGGA
    GACATTGGCC CTCCAGGGGA CAATGGCCCT GAAGGCATGA AGGGTAAGCC TGGAGCCCGA
    GGCCTGCCTG GACCTCCTGG GCAGCTGGGG CCTGAGGGAG ATGAAGGACC CATGGGGCCA
    CCAGGAGTCC CAGGCTTGGA GGGTCAGCCT GGAAGGAAAG GGTTTCCTGG GAGGCCTGGC
    CTGGATGGCT CGAAGGGGGA ACCAGGGGAT CCTGGACGGC CAGGACCTGT GGGTGAACAG
    GGGCTTATGG GATTCATTGG TCTTGTCGGA GAGCCTGGAA TTGTTGGGGA GAAGGGAGAC
    CGTGGGGTGA TGGGACCCCC AGGTGCACCT GGACCCAAGG GGTCGATGGG CCATCCTGGA
    ACACCCGGTG GTATTGGGAA CCCTGGAGAG CCTGGACCCT GGGGCCCTCC AGGATCTCGA
    GGCCTGCCAG GCATGAGGGG AGCCAAGGGG CACCGGGGCC CTCGAGGACC TGATGGACCA
    GCTGGGGAGC AGGGATCCAA GGGCCTGAAG GGTCGTGTAG GACCTCGGGG CAGACCTGGC
    CAGCCAGGAC AGCAGGGTGC AGCTGGTGAG CGAGGCCACT CAGGTGCAAA AGGCTTCCTT
    GGCATCCCTG GTCCCTCAGG CCCTCCAGGT GCCAAGGGCC TCCCAGGAGA ACCGGGCTCT
    CAGGGACCTC AGGGGCCAGT TGGTCCTCCA GGAGAAATGG GGCCCAAGGG ACCGCCGGGT
    GCAGTGGGAG AGCCTGGCCT TCCTGGGGAC TCCGGAATGA AGGGTGACCT CGGACCTCTG
    GGCCCCCCTG GGGAGCAAGG TCTTATTGGG CAGCGGGGAG AGCCAGGCCT TGAGGGAGAC
    CATGGCCCTG TGGGGCCAGA TGGGCTGAAG GGCGACAGGG GCGACCCAGG GCCCGATGGC
    GAGCATGGTG AAAAGGGCCA GGAAGGGCTG AAAGGAGAGG ATGGGTCCCC TGGGCCCCCG
    GGCATCACTG GCGTCCCGGG TCGTGAGGGG AAGCCGGGCA AGCAGGGTGA GAAAGGCCAG
    AGGGGAGCTA AGGGTGCCAA GGGCCACCAA GGATATCTGG GAGAGATGGG CATCCCCGGA
    GAACCTGGGC CCCCTGGGAC CCCAGGTCCT AAAGGGTCCC GGGGCACCCT AGGACCAACG
    GGTGCTCCAG GACGGATGGG AGCCCAAGGA GAACCAGGAT TGGCCGGTTA TAATGGACAC
    AAAGGCATCA CAGGACCCCT CGGGCCTCCC GGGCCCAAAG GCGAGAAGGG GGACCAGGGC
    GAGGATGGCA AGACCGAGGG GCCCCCTGGG CCACCAGGAG ATCGGGGTCC TGTGGGTGAT
    CGAGGAGACC GTGGGGAGCC AGGCGACCCT GGATACCCTG GTCAAGAGGG AGTTCAAGGC
    CTCCGTGGAG AACCGGGCCA GCAGGGACAG CCTGGGCATC CAGGACCCCG GGGGCGCCCA
    GGACCCAAAG GATCAAAAGG CGAAGAGGGC CCAAAGGGAA AGCCAGGCAA GGCTGGGCCA
    TCAGGCCGGA GGGGGACCCA GGGCCTTCAA GGACTGCCAG GCCCCCGAGG CGTGGTGGGG
    AGACAGGGCC CTGAGGGCAC TGCTGGATCT GATGGGATTC CTGGCAGAGA TGGTCGGCCA
    GGATATCAGG GAGACCAGGG AAATGATGGG GACCCTGGCC CTGTGGGCCC TGCTGGGAGA
    AGAGGAAATC CAGGTGTGGC TGGCTTGCCT GGAGCACAGG GGCCTCCGGG ATTCAAGGGT
    GAAAGTGGGT TACCTGGGCA ACTGGGTCCC CCTGGAAAAC GAGGGACAGA AGGTGGAACA
    GGGCTTCCTG GGAACCAGGG GGAGCCAGGA TCCAAAGGCC AGCCGGGTGA CTCTGGCGAG
    ATGGGCTTCC CAGGAGTGGC TGGCCTCTTT GGACCCAAGG GCCCCCCTGG AGACATTGGC
    TTCAAAGGCA TCCAAGGTCC TCGGGGGCCT CCTGGCTTGA TGGGAAAGGA AGGTATCATT
    GGCCCCCCCG GAATGCTGGG ACCTTCTGGA CTCCCGGGTC CCAAAGGTGA CAGAGGCAGC
    CGAGGAGACT TGGGACTGCA AGGCCCAAGG GGTCCTCCTG GTCCAAGGGG CCGGCCAGGT
    CCCCCGGGCC CTCCTTGGCA TCCCATCCAG TTTCAGCAAG ATGACCTTGG AGCAGCTTTC
    CAGACATGGA TGGATGCTCA AGGAGCCGTC AGATCGGAGC AGGGGTACAG CTATCCGGAC
    CAGCTGGCGC TAGACCAGGG AGGGGAGATC TTCAAAACCT TACACTACCT CAGCAACCTC
    ATCCAGAGCA TTAAGACACC CTTGGGCACC AAGGAGAACC CAGCCCGGGT CTGCCGGGAC
    CTCATGGACT GTGAGCAGAG GATGGCGGAT GGTACCTACT GGGTGGACCC CAACCTCGGC
    TGCTCCTCTG ACACCATTGA AGTCTCCTGC AACTTTACAC AGGGTGGGCA GACGTGCCTG
    AAGCCCATCA CGGCCTCCAA GGCCGAGTTT GCTGTGAGTC GGGTCCAGAT GAATTTCTTG
    CACCTGCTGA GCTCTGAGGG GACACAGCAT ATCACAATCC ACTGTCTGAA CATGACGGTG
    TGGCAGGAGG GACCGGGACG CTCCTCTGCC AGACAGGCTG TGCGCTTCCG TGCCTGGAAC
    GGACAGGTCT TCGAAGCTGG GGGTCAGTTC AGGCCAGAGG TGTCTATGGA TGGCTGCAAG
    GTCCATGATG GCCGCTGGCA TCAGACACTG TTCACCTTCC GGACCCAGGA CCCCCAGCAG
    CTGCCCATCG TCAGTGTGGA CAATCTCCCG CCTGTCTCAT CAGGGAAGCA GTACCGCCTG
    GAAGTTGGAC CTGCGTGCTT CCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030109492.1
    CDS90..3356
    Translation

    Target ORF information:

    RefSeq Version XM_030253632.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030253632.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGAGAGCG GGGTCTCCCT GGCGTTCCTG GCAAGAGGGG CGAGATGGGG CTTCCCTGGA 
    GACTTTGGGG AAAGAGGCCC CCCTGGACTT GATGGCAACC CTGGAGAAAT TGGCCTGCCG
    GGGCCACCAG GAGTGCTCGG GCTCATTGGT GACACAGGAG CACTGGGCCC AGTTGGCTAC
    CCAGGACCGA AAGGCATGAA GGGACTGATG GGCGGCGTGG GGGAGCCCGG ACTGAAAGGT
    GATAAGGGTG AACAAGGGGT GCCAGGTGTG TCGGGAGATC CTGGCTTCCA AGGAGACAAG
    GGGAGTCATG GCTTGCCAGG GCTCCCGGGT GGCCGGGGGA AGCCAGGGCC TCTGGGAAAA
    GCTGGAGACA AAGGATCACT TGGGTTTCCT GGACCTCCTG GACCTGAGGG TTTCCCAGGA
    GACATTGGCC CTCCAGGGGA CAATGGCCCT GAAGGCATGA AGGGTAAGCC TGGAGCCCGA
    GGCCTGCCTG GACCTCCTGG GCAGCTGGGG CCTGAGGGAG ATGAAGGACC CATGGGGCCA
    CCAGGAGTCC CAGGCTTGGA GGGTCAGCCT GGAAGGAAAG GGTTTCCTGG GAGGCCTGGC
    CTGGATGGCT CGAAGGGGGA ACCAGGGGAT CCTGGACGGC CAGGACCTGT GGGTGAACAG
    GGGCTTATGG GATTCATTGG TCTTGTCGGA GAGCCTGGAA TTGTTGGGGA GAAGGGAGAC
    CGTGGGGTGA TGGGACCCCC AGGTGCACCT GGACCCAAGG GGTCGATGGG CCATCCTGGA
    ACACCCGGTG GTATTGGGAA CCCTGGAGAG CCTGGACCCT GGGGCCCTCC AGGATCTCGA
    GGCCTGCCAG GCATGAGGGG AGCCAAGGGG CACCGGGGCC CTCGAGGACC TGATGGACCA
    GCTGGGGAGC AGGGATCCAA GGGCCTGAAG GGTCGTGTAG GACCTCGGGG CAGACCTGGC
    CAGCCAGGAC AGCAGGGTGC AGCTGGTGAG CGAGGCCACT CAGGTGCAAA AGGCTTCCTT
    GGCATCCCTG GTCCCTCAGG CCCTCCAGGT GCCAAGGGCC TCCCAGGAGA ACCGGGCTCT
    CAGGGACCTC AGGGGCCAGT TGGTCCTCCA GGAGAAATGG GGCCCAAGGG ACCGCCGGGT
    GCAGTGGGAG AGCCTGGCCT TCCTGGGGAC TCCGGAATGA AGGGTGACCT CGGACCTCTG
    GGCCCCCCTG GGGAGCAAGG TCTTATTGGG CAGCGGGGAG AGCCAGGCCT TGAGGGAGAC
    CATGGCCCTG TGGGGCCAGA TGGGCTGAAG GGCGACAGGG GCGACCCAGG GCCCGATGGC
    GAGCATGGTG AAAAGGGCCA GGAAGGGCTG AAAGGAGAGG ATGGGTCCCC TGGGCCCCCG
    GGCATCACTG GCGTCCCGGG TCGTGAGGGG AAGCCGGGCA AGCAGGGTGA GAAAGGCCAG
    AGGGGAGCTA AGGGTGCCAA GGGCCACCAA GGATATCTGG GAGAGATGGG CATCCCCGGA
    GAACCTGGGC CCCCTGGGAC CCCAGGTCCT AAAGGGTCCC GGGGCACCCT AGGACCAACG
    GGTGCTCCAG GACGGATGGG AGCCCAAGGA GAACCAGGAT TGGCCGGTTA TAATGGACAC
    AAAGGCATCA CAGGACCCCT CGGGCCTCCC GGGCCCAAAG GCGAGAAGGG GGACCAGGGC
    GAGGATGGCA AGACCGAGGG GCCCCCTGGG CCACCAGGAG ATCGGGGTCC TGTGGGTGAT
    CGAGGAGACC GTGGGGAGCC AGGCGACCCT GGATACCCTG GTCAAGAGGG AGTTCAAGGC
    CTCCGTGGAG AACCGGGCCA GCAGGGACAG CCTGGGCATC CAGGACCCCG GGGGCGCCCA
    GGACCCAAAG GATCAAAAGG CGAAGAGGGC CCAAAGGGAA AGCCAGGCAA GGCTGGGCCA
    TCAGGCCGGA GGGGGACCCA GGGCCTTCAA GGACTGCCAG GCCCCCGAGG CGTGGTGGGG
    AGACAGGGCC CTGAGGGCAC TGCTGGATCT GATGGGATTC CTGGCAGAGA TGGTCGGCCA
    GGATATCAGG GAGACCAGGG AAATGATGGG GACCCTGGCC CTGTGGGCCC TGCTGGGAGA
    AGAGGAAATC CAGGTGTGGC TGGCTTGCCT GGAGCACAGG GGCCTCCGGG ATTCAAGGGT
    GAAAGTGGGT TACCTGGGCA ACTGGGTCCC CCTGGAAAAC GAGGGACAGA AGGTGGAACA
    GGGCTTCCTG GGAACCAGGG GGAGCCAGGA TCCAAAGGCC AGCCGGGTGA CTCTGGCGAG
    ATGGGCTTCC CAGGAGTGGC TGGCCTCTTT GGACCCAAGG GCCCCCCTGG AGACATTGGC
    TTCAAAGGCA TCCAAGGTCC TCGGGGGCCT CCTGGCTTGA TGGGAAAGGA AGGTATCATT
    GGCCCCCCCG GAATGCTGGG ACCTTCTGGA CTCCCGGGTC CCAAAGGTGA CAGAGGCAGC
    CGAGGAGACT TGGGACTGCA AGGCCCAAGG GGTCCTCCTG GTCCAAGGGG CCGGCCAGGT
    CCCCCGGGCC CTCCTTGGCA TCCCATCCAG TTTCAGCAAG ATGACCTTGG AGCAGCTTTC
    CAGACATGGA TGGATGCTCA AGGAGCCGTC AGATCGGAGC AGGGGTACAG CTATCCGGAC
    CAGCTGGCGC TAGACCAGGG AGGGGAGATC TTCAAAACCT TACACTACCT CAGCAACCTC
    ATCCAGAGCA TTAAGACACC CTTGGGCACC AAGGAGAACC CAGCCCGGGT CTGCCGGGAC
    CTCATGGACT GTGAGCAGAG GATGGCGGAT GGTACCTACT GGGTGGACCC CAACCTCGGC
    TGCTCCTCTG ACACCATTGA AGTCTCCTGC AACTTTACAC AGGGTGGGCA GACGTGCCTG
    AAGCCCATCA CGGCCTCCAA GGCCGAGTTT GCTGTGAGTC GGGTCCAGAT GAATTTCTTG
    CACCTGCTGA GCTCTGAGGG GACACAGCAT ATCACAATCC ACTGTCTGAA CATGACGGTG
    TGGCAGGAGG GACCGGGACG CTCCTCTGCC AGACAGGCTG TGCGCTTCCG TGCCTGGAAC
    GGACAGGTCT TCGAAGCTGG GGGTCAGTTC AGGCCAGAGG TGTCTATGGA TGGCTGCAAG
    GTCCATGATG GCCGCTGGCA TCAGACACTG TTCACCTTCC GGACCCAGGA CCCCCAGCAG
    CTGCCCATCG TCAGTGTGGA CAATCTCCCG CCTGTCTCAT CAGGGAAGCA GTACCGCCTG
    GAAGTTGGAC CTGCGTGCTT CCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu88568
    Clone ID Related Accession (Same CDS sequence) XM_030253633.1
    Accession Version XM_030253633.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3231bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product collagen alpha-1(XXVII) chain isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGGTAGGC CGGGCTTCCC TGGAGACTTT GGGGAAAGAG GCCCCCCTGG ACTTGATGGC 
    AACCCTGGAG AAATTGGCCT GCCGGGGCCA CCAGGAGTGC TCGGGCTCAT TGGTGACACA
    GGAGCACTGG GCCCAGTTGG CTACCCAGGA CCGAAAGGCA TGAAGGGACT GATGGGCGGC
    GTGGGGGAGC CCGGACTGAA AGGTGATAAG GGTGAACAAG GGGTGCCAGG TGTGTCGGGA
    GATCCTGGCT TCCAAGGAGA CAAGGGGAGT CATGGCTTGC CAGGGCTCCC GGGTGGCCGG
    GGGAAGCCAG GGCCTCTGGG AAAAGCTGGA GACAAAGGAT CACTTGGGTT TCCTGGACCT
    CCTGGACCTG AGGGTTTCCC AGGAGACATT GGCCCTCCAG GGGACAATGG CCCTGAAGGC
    ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG CCTGGACCTC CTGGGCAGCT GGGGCCTGAG
    GGAGATGAAG GACCCATGGG GCCACCAGGA GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG
    AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT GGCTCGAAGG GGGAACCAGG GGATCCTGGA
    CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT ATGGGATTCA TTGGTCTTGT CGGAGAGCCT
    GGAATTGTTG GGGAGAAGGG AGACCGTGGG GTGATGGGAC CCCCAGGTGC ACCTGGACCC
    AAGGGGTCGA TGGGCCATCC TGGAACACCC GGTGGTATTG GGAACCCTGG AGAGCCTGGA
    CCCTGGGGCC CTCCAGGATC TCGAGGCCTG CCAGGCATGA GGGGAGCCAA GGGGCACCGG
    GGCCCTCGAG GACCTGATGG ACCAGCTGGG GAGCAGGGAT CCAAGGGCCT GAAGGGTCGT
    GTAGGACCTC GGGGCAGACC TGGCCAGCCA GGACAGCAGG GTGCAGCTGG TGAGCGAGGC
    CACTCAGGTG CAAAAGGCTT CCTTGGCATC CCTGGTCCCT CAGGCCCTCC AGGTGCCAAG
    GGCCTCCCAG GAGAACCGGG CTCTCAGGGA CCTCAGGGGC CAGTTGGTCC TCCAGGAGAA
    ATGGGGCCCA AGGGACCGCC GGGTGCAGTG GGAGAGCCTG GCCTTCCTGG GGACTCCGGA
    ATGAAGGGTG ACCTCGGACC TCTGGGCCCC CCTGGGGAGC AAGGTCTTAT TGGGCAGCGG
    GGAGAGCCAG GCCTTGAGGG AGACCATGGC CCTGTGGGGC CAGATGGGCT GAAGGGCGAC
    AGGGGCGACC CAGGGCCCGA TGGCGAGCAT GGTGAAAAGG GCCAGGAAGG GCTGAAAGGA
    GAGGATGGGT CCCCTGGGCC CCCGGGCATC ACTGGCGTCC CGGGTCGTGA GGGGAAGCCG
    GGCAAGCAGG GTGAGAAAGG CCAGAGGGGA GCTAAGGGTG CCAAGGGCCA CCAAGGATAT
    CTGGGAGAGA TGGGCATCCC CGGAGAACCT GGGCCCCCTG GGACCCCAGG TCCTAAAGGG
    TCCCGGGGCA CCCTAGGACC AACGGGTGCT CCAGGACGGA TGGGAGCCCA AGGAGAACCA
    GGATTGGCCG GTTATAATGG ACACAAAGGC ATCACAGGAC CCCTCGGGCC TCCCGGGCCC
    AAAGGCGAGA AGGGGGACCA GGGCGAGGAT GGCAAGACCG AGGGGCCCCC TGGGCCACCA
    GGAGATCGGG GTCCTGTGGG TGATCGAGGA GACCGTGGGG AGCCAGGCGA CCCTGGATAC
    CCTGGTCAAG AGGGAGTTCA AGGCCTCCGT GGAGAACCGG GCCAGCAGGG ACAGCCTGGG
    CATCCAGGAC CCCGGGGGCG CCCAGGACCC AAAGGATCAA AAGGCGAAGA GGGCCCAAAG
    GGAAAGCCAG GCAAGGCTGG GCCATCAGGC CGGAGGGGGA CCCAGGGCCT TCAAGGACTG
    CCAGGCCCCC GAGGCGTGGT GGGGAGACAG GGCCCTGAGG GCACTGCTGG ATCTGATGGG
    ATTCCTGGCA GAGATGGTCG GCCAGGATAT CAGGGAGACC AGGGAAATGA TGGGGACCCT
    GGCCCTGTGG GCCCTGCTGG GAGAAGAGGA AATCCAGGTG TGGCTGGCTT GCCTGGAGCA
    CAGGGGCCTC CGGGATTCAA GGGTGAAAGT GGGTTACCTG GGCAACTGGG TCCCCCTGGA
    AAACGAGGGA CAGAAGGTGG AACAGGGCTT CCTGGGAACC AGGGGGAGCC AGGATCCAAA
    GGCCAGCCGG GTGACTCTGG CGAGATGGGC TTCCCAGGAG TGGCTGGCCT CTTTGGACCC
    AAGGGCCCCC CTGGAGACAT TGGCTTCAAA GGCATCCAAG GTCCTCGGGG GCCTCCTGGC
    TTGATGGGAA AGGAAGGTAT CATTGGCCCC CCCGGAATGC TGGGACCTTC TGGACTCCCG
    GGTCCCAAAG GTGACAGAGG CAGCCGAGGA GACTTGGGAC TGCAAGGCCC AAGGGGTCCT
    CCTGGTCCAA GGGGCCGGCC AGGTCCCCCG GGCCCTCCTT GGCATCCCAT CCAGTTTCAG
    CAAGATGACC TTGGAGCAGC TTTCCAGACA TGGATGGATG CTCAAGGAGC CGTCAGATCG
    GAGCAGGGGT ACAGCTATCC GGACCAGCTG GCGCTAGACC AGGGAGGGGA GATCTTCAAA
    ACCTTACACT ACCTCAGCAA CCTCATCCAG AGCATTAAGA CACCCTTGGG CACCAAGGAG
    AACCCAGCCC GGGTCTGCCG GGACCTCATG GACTGTGAGC AGAGGATGGC GGATGGTACC
    TACTGGGTGG ACCCCAACCT CGGCTGCTCC TCTGACACCA TTGAAGTCTC CTGCAACTTT
    ACACAGGGTG GGCAGACGTG CCTGAAGCCC ATCACGGCCT CCAAGGCCGA GTTTGCTGTG
    AGTCGGGTCC AGATGAATTT CTTGCACCTG CTGAGCTCTG AGGGGACACA GCATATCACA
    ATCCACTGTC TGAACATGAC GGTGTGGCAG GAGGGACCGG GACGCTCCTC TGCCAGACAG
    GCTGTGCGCT TCCGTGCCTG GAACGGACAG GTCTTCGAAG CTGGGGGTCA GTTCAGGCCA
    GAGGTGTCTA TGGATGGCTG CAAGGTCCAT GATGGCCGCT GGCATCAGAC ACTGTTCACC
    TTCCGGACCC AGGACCCCCA GCAGCTGCCC ATCGTCAGTG TGGACAATCT CCCGCCTGTC
    TCATCAGGGA AGCAGTACCG CCTGGAAGTT GGACCTGCGT GCTTCCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030109493.1
    CDS2811..6041
    Translation

    Target ORF information:

    RefSeq Version XM_030253633.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030253633.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGGTAGGC CGGGCTTCCC TGGAGACTTT GGGGAAAGAG GCCCCCCTGG ACTTGATGGC 
    AACCCTGGAG AAATTGGCCT GCCGGGGCCA CCAGGAGTGC TCGGGCTCAT TGGTGACACA
    GGAGCACTGG GCCCAGTTGG CTACCCAGGA CCGAAAGGCA TGAAGGGACT GATGGGCGGC
    GTGGGGGAGC CCGGACTGAA AGGTGATAAG GGTGAACAAG GGGTGCCAGG TGTGTCGGGA
    GATCCTGGCT TCCAAGGAGA CAAGGGGAGT CATGGCTTGC CAGGGCTCCC GGGTGGCCGG
    GGGAAGCCAG GGCCTCTGGG AAAAGCTGGA GACAAAGGAT CACTTGGGTT TCCTGGACCT
    CCTGGACCTG AGGGTTTCCC AGGAGACATT GGCCCTCCAG GGGACAATGG CCCTGAAGGC
    ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG CCTGGACCTC CTGGGCAGCT GGGGCCTGAG
    GGAGATGAAG GACCCATGGG GCCACCAGGA GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG
    AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT GGCTCGAAGG GGGAACCAGG GGATCCTGGA
    CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT ATGGGATTCA TTGGTCTTGT CGGAGAGCCT
    GGAATTGTTG GGGAGAAGGG AGACCGTGGG GTGATGGGAC CCCCAGGTGC ACCTGGACCC
    AAGGGGTCGA TGGGCCATCC TGGAACACCC GGTGGTATTG GGAACCCTGG AGAGCCTGGA
    CCCTGGGGCC CTCCAGGATC TCGAGGCCTG CCAGGCATGA GGGGAGCCAA GGGGCACCGG
    GGCCCTCGAG GACCTGATGG ACCAGCTGGG GAGCAGGGAT CCAAGGGCCT GAAGGGTCGT
    GTAGGACCTC GGGGCAGACC TGGCCAGCCA GGACAGCAGG GTGCAGCTGG TGAGCGAGGC
    CACTCAGGTG CAAAAGGCTT CCTTGGCATC CCTGGTCCCT CAGGCCCTCC AGGTGCCAAG
    GGCCTCCCAG GAGAACCGGG CTCTCAGGGA CCTCAGGGGC CAGTTGGTCC TCCAGGAGAA
    ATGGGGCCCA AGGGACCGCC GGGTGCAGTG GGAGAGCCTG GCCTTCCTGG GGACTCCGGA
    ATGAAGGGTG ACCTCGGACC TCTGGGCCCC CCTGGGGAGC AAGGTCTTAT TGGGCAGCGG
    GGAGAGCCAG GCCTTGAGGG AGACCATGGC CCTGTGGGGC CAGATGGGCT GAAGGGCGAC
    AGGGGCGACC CAGGGCCCGA TGGCGAGCAT GGTGAAAAGG GCCAGGAAGG GCTGAAAGGA
    GAGGATGGGT CCCCTGGGCC CCCGGGCATC ACTGGCGTCC CGGGTCGTGA GGGGAAGCCG
    GGCAAGCAGG GTGAGAAAGG CCAGAGGGGA GCTAAGGGTG CCAAGGGCCA CCAAGGATAT
    CTGGGAGAGA TGGGCATCCC CGGAGAACCT GGGCCCCCTG GGACCCCAGG TCCTAAAGGG
    TCCCGGGGCA CCCTAGGACC AACGGGTGCT CCAGGACGGA TGGGAGCCCA AGGAGAACCA
    GGATTGGCCG GTTATAATGG ACACAAAGGC ATCACAGGAC CCCTCGGGCC TCCCGGGCCC
    AAAGGCGAGA AGGGGGACCA GGGCGAGGAT GGCAAGACCG AGGGGCCCCC TGGGCCACCA
    GGAGATCGGG GTCCTGTGGG TGATCGAGGA GACCGTGGGG AGCCAGGCGA CCCTGGATAC
    CCTGGTCAAG AGGGAGTTCA AGGCCTCCGT GGAGAACCGG GCCAGCAGGG ACAGCCTGGG
    CATCCAGGAC CCCGGGGGCG CCCAGGACCC AAAGGATCAA AAGGCGAAGA GGGCCCAAAG
    GGAAAGCCAG GCAAGGCTGG GCCATCAGGC CGGAGGGGGA CCCAGGGCCT TCAAGGACTG
    CCAGGCCCCC GAGGCGTGGT GGGGAGACAG GGCCCTGAGG GCACTGCTGG ATCTGATGGG
    ATTCCTGGCA GAGATGGTCG GCCAGGATAT CAGGGAGACC AGGGAAATGA TGGGGACCCT
    GGCCCTGTGG GCCCTGCTGG GAGAAGAGGA AATCCAGGTG TGGCTGGCTT GCCTGGAGCA
    CAGGGGCCTC CGGGATTCAA GGGTGAAAGT GGGTTACCTG GGCAACTGGG TCCCCCTGGA
    AAACGAGGGA CAGAAGGTGG AACAGGGCTT CCTGGGAACC AGGGGGAGCC AGGATCCAAA
    GGCCAGCCGG GTGACTCTGG CGAGATGGGC TTCCCAGGAG TGGCTGGCCT CTTTGGACCC
    AAGGGCCCCC CTGGAGACAT TGGCTTCAAA GGCATCCAAG GTCCTCGGGG GCCTCCTGGC
    TTGATGGGAA AGGAAGGTAT CATTGGCCCC CCCGGAATGC TGGGACCTTC TGGACTCCCG
    GGTCCCAAAG GTGACAGAGG CAGCCGAGGA GACTTGGGAC TGCAAGGCCC AAGGGGTCCT
    CCTGGTCCAA GGGGCCGGCC AGGTCCCCCG GGCCCTCCTT GGCATCCCAT CCAGTTTCAG
    CAAGATGACC TTGGAGCAGC TTTCCAGACA TGGATGGATG CTCAAGGAGC CGTCAGATCG
    GAGCAGGGGT ACAGCTATCC GGACCAGCTG GCGCTAGACC AGGGAGGGGA GATCTTCAAA
    ACCTTACACT ACCTCAGCAA CCTCATCCAG AGCATTAAGA CACCCTTGGG CACCAAGGAG
    AACCCAGCCC GGGTCTGCCG GGACCTCATG GACTGTGAGC AGAGGATGGC GGATGGTACC
    TACTGGGTGG ACCCCAACCT CGGCTGCTCC TCTGACACCA TTGAAGTCTC CTGCAACTTT
    ACACAGGGTG GGCAGACGTG CCTGAAGCCC ATCACGGCCT CCAAGGCCGA GTTTGCTGTG
    AGTCGGGTCC AGATGAATTT CTTGCACCTG CTGAGCTCTG AGGGGACACA GCATATCACA
    ATCCACTGTC TGAACATGAC GGTGTGGCAG GAGGGACCGG GACGCTCCTC TGCCAGACAG
    GCTGTGCGCT TCCGTGCCTG GAACGGACAG GTCTTCGAAG CTGGGGGTCA GTTCAGGCCA
    GAGGTGTCTA TGGATGGCTG CAAGGTCCAT GATGGCCGCT GGCATCAGAC ACTGTTCACC
    TTCCGGACCC AGGACCCCCA GCAGCTGCCC ATCGTCAGTG TGGACAATCT CCCGCCTGTC
    TCATCAGGGA AGCAGTACCG CCTGGAAGTT GGACCTGCGT GCTTCCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu33009
    Clone ID Related Accession (Same CDS sequence) XM_006538039.3 , XM_006538039.4
    Accession Version XM_006538039.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3186bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product collagen alpha-1(XXVII) chain isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006538039.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGGCACGG GATTCGCGCG GGGGGCCCGA GGCACAGCGG CGTCAGGACC CGGGGGGGGG 
    TTTCTCTTCG CCTGGATCTT GGTCTCATTT ACCTGTCACC TGGCCTCCAC CCAAGGAGCT
    CCTGAAGATG TGGATGTCCT CCAGCGGCTG GGCCTCAGCT GGACGAAGGC CGGGGGTGGC
    CGGAGTCCTA CACCCCCTGG TGTCATTCCT TTCCCATCTG GCTTCATCTT CACACAGCGG
    GCCAAGCTGC AGGCCCCCAC AGCCAATGTT CTTCCCACCA CCCTGGGGCG GGAGCTGGCA
    TTGGTGTTGA GCCTCTGTTC ACATCGTGTG AACCATGCCT TCCTCTTTGC CATCCGCAGC
    AGGAAACACA AGCTGCAACT GGGTCTGCAG TTCCTCCCCG GCAGGACGAT CATCCACCTG
    GGGCCTCGGC AATCAGTGGC CTTCGACTTG GATGTGCATG ATGGGCGCTG GCACCACCTG
    GCCCTAGAGT TGCGTGGCCG CACAGTCACA ATGGTGACAG CCTGTGGGCA GCACAGGGTA
    CCTGTCCCAC TGCCTTCCCG TAGGGACTCC ATGCTTGACC CCCAGGGCTC CTTTCTCTTG
    GGGAAGGTGA ACCCCCGTGC TGTCCAGTTT GAAGGTGCAC TCTGTCAGTT CAGCATCCAC
    CCTGTGGCGC AGGTCGCTCA CAATTACTGT GCCCACCTGA GAGAGCGGTG CCGACAGGTG
    GACACATATA GTCCCCAGGT GGGAACCCTC TTTCCTTGGG ACTCTGGCCC AGCCTTTGCT
    TTGCATCCTG AACCAGCCTT GCTTGGCTTG GGGAATCTGA CCAGAACTCC AGCAACCCTG
    GGGGCCAGGC CTGTAAGCAG GGCACTTGCA GTGACTCTGG CTCCTGCCAT GCCCACCAAG
    CCCCTGAGGA CTGTCCACCC AGATGTCTCT GAACACAGCT CTTCCCAGAC CCCACTGTCA
    CCTGCCAAAC AGTCGGCCAG GAAAACACCT TCCCCCTCCT CGTCAGCTTC CCTGGCTAAT
    TCCACCAGGG TTTATCGTCC AGCTGCTGCC CAGCCAAGAC AGATCACAAC TACTAGCCCC
    ACCAAACGTT CCCCCACCAA GCCTTCTGTT TCTCCCCTTT CAGTTACCCC CATGAAAAGT
    CCCCATGCAA CCCAGAAAAC AGGTGTCCCT TCATTCACAA AGCCTGTTCC ACCCACTCAG
    AAGCCAGCGC CCTTCACCTC CTACCTAGCA CCTTCCAAAG CCTCTAGTCC CACTGTGAGG
    CCTGTTCAGA AGACTTTCAT GACACCCCGA CCTCCAGTCC CCAGCCCCCA GCCCCTGCGC
    CCTACTACTG GCTTATCTAA GAAGTTCACT AACCCCACTG TTGCAAAATC TAAGTCCAAG
    ACGACCAGTT GGGCCAGCAA GCCGGTCTTG GCCCGCTCCA GTGTCCCTAA AACGCTTCAG
    CAAACTGTTT TGTCCCAGTC TCCTGTTTCC TATCTGGGCT CTCAGACACT GGCTCCAGCC
    CTCCCTCCTT TGGGTGTTGG AAATCCTAGA ACGATGCCTC CCACACGTGA CTCTGCTTTA
    ACTCCTGCTG GAAGCAAGAA ATTCACAGGA CGGGAAACCT CCAAGAAAAC CAGACAGAAG
    AGCAGCCCCC GGAAGCCCGA ACCTCTCAGC CCTGGGAAGT CAGCCAGGGA TGCCTCACCA
    AGAGACCTGA CAACCAAGCC TAGCCGGCCG TCCACCCCAG CCCTGGTCCT TGCCCCAGCT
    TACCTCCTGT CCTCCAGCCC CCAGCCCACC AGCAGCAGCT TCCCTTTCTT CCACCTGCTG
    GGCCCTACAC CTTTCCCGAT GCTGATGGGG CCTCCAGGCT CCAAGGGAGA CTGTGGCTTG
    CCGGGTCCCC CTGGGCTTCC AGGATTACCT GGATCTCCGG GCGCACGAGG TCCTCGGGGT
    CCTCCTGGGC CGTACGGGAA CCCAGGCCCA CCTGGCCCTC CAGGAGCCAA AGGACAGAAA
    GGGGACCCAG GACTCTCACC AGGACAGGCT CACGATGGAG CAAAGGGCAA CATGGGCTTG
    CCTGGGCTCT CCGGAAATCC AGGACCCTTG GGAAGGAAGG GACACAAGGG CCATCCTGGA
    GCAGCAGGGC ACCCTGGAGA ACAGGGGCAG CCAGGACCTG AGGGCAGTCC AGGGGCCAAA
    GGTTACCCTG GCAGGCAGGG GTTCCCTGGA CCTGTAGGAG ACCCTGGCCC CAAAGGCAGC
    AGGGGTTACA TTGGTCTTCC GGGGCTCTTT GGCCTGCCAG GGTCGGATGG AGAGCGGGGT
    CTCCCTGGCG TTCCTGGCAA GAGGGGCGAG ATGGGTAGGC CGGGCTTCCC TGGAGACTTT
    GGGGAAAGAG GCCCCCCTGG ACTTGATGGC AACCCTGGAG AAATTGGCCT GCCGGGGCCA
    CCAGGAGTGC TCGGGCTCAT TGGTGACACA GGAGCACTGG GCCCAGTTGG CTACCCAGGA
    CCGAAAGGCA TGAAGGGACT GATGGGCGGC GTGGGGGAGC CCGGACTGAA AGGTGATAAG
    GGTGAACAAG GGGTGCCAGG TGTGTCGGGA GATCCTGGCT TCCAAGGAGA CAAGGGGAGT
    CATGGCTTGC CAGGGCTCCC GGGTGGCCGG GGGAAGCCAG GGCCTCTGGG AAAAGCTGGA
    GACAAAGGAT CACTTGGGTT TCCTGGACCT CCTGGACCTG AGGGTTTCCC AGGAGACATT
    GGCCCTCCAG GGGACAATGG CCCTGAAGGC ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG
    CCTGGACCTC CTGGGCAGCT GGGGCCTGAG GGAGATGAAG GACCCATGGG GCCACCAGGA
    GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT
    GGCTCGAAGG GGGAACCAGG GGATCCTGGA CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT
    ATGGGATTCA TTGGTCTTGT CGGAGAGCCT GGAATTGTTG GGGAGAAGGG AGACCGTGGG
    GTGATGGGAC CCCCAGGTGC ACCTGGACCC AAGGGGTCGA TGGTAAGGAG GCCATCCTGG
    AACACCCGGT GGTATTGGGA ACCCTGGAGA GCCTGGACCC TGGGGCCCTC CAGGATCTCG
    AGGCCTGCCA GGCATGAGGG GAGCCAAGGG GCACCGGGGT CTCCAGCTGA CTTACACAGG
    CTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006538102.1
    CDS509..3694
    Translation

    Target ORF information:

    RefSeq Version XM_006538039.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006538039.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGGCACGG GATTCGCGCG GGGGGCCCGA GGCACAGCGG CGTCAGGACC CGGGGGGGGG 
    TTTCTCTTCG CCTGGATCTT GGTCTCATTT ACCTGTCACC TGGCCTCCAC CCAAGGAGCT
    CCTGAAGATG TGGATGTCCT CCAGCGGCTG GGCCTCAGCT GGACGAAGGC CGGGGGTGGC
    CGGAGTCCTA CACCCCCTGG TGTCATTCCT TTCCCATCTG GCTTCATCTT CACACAGCGG
    GCCAAGCTGC AGGCCCCCAC AGCCAATGTT CTTCCCACCA CCCTGGGGCG GGAGCTGGCA
    TTGGTGTTGA GCCTCTGTTC ACATCGTGTG AACCATGCCT TCCTCTTTGC CATCCGCAGC
    AGGAAACACA AGCTGCAACT GGGTCTGCAG TTCCTCCCCG GCAGGACGAT CATCCACCTG
    GGGCCTCGGC AATCAGTGGC CTTCGACTTG GATGTGCATG ATGGGCGCTG GCACCACCTG
    GCCCTAGAGT TGCGTGGCCG CACAGTCACA ATGGTGACAG CCTGTGGGCA GCACAGGGTA
    CCTGTCCCAC TGCCTTCCCG TAGGGACTCC ATGCTTGACC CCCAGGGCTC CTTTCTCTTG
    GGGAAGGTGA ACCCCCGTGC TGTCCAGTTT GAAGGTGCAC TCTGTCAGTT CAGCATCCAC
    CCTGTGGCGC AGGTCGCTCA CAATTACTGT GCCCACCTGA GAGAGCGGTG CCGACAGGTG
    GACACATATA GTCCCCAGGT GGGAACCCTC TTTCCTTGGG ACTCTGGCCC AGCCTTTGCT
    TTGCATCCTG AACCAGCCTT GCTTGGCTTG GGGAATCTGA CCAGAACTCC AGCAACCCTG
    GGGGCCAGGC CTGTAAGCAG GGCACTTGCA GTGACTCTGG CTCCTGCCAT GCCCACCAAG
    CCCCTGAGGA CTGTCCACCC AGATGTCTCT GAACACAGCT CTTCCCAGAC CCCACTGTCA
    CCTGCCAAAC AGTCGGCCAG GAAAACACCT TCCCCCTCCT CGTCAGCTTC CCTGGCTAAT
    TCCACCAGGG TTTATCGTCC AGCTGCTGCC CAGCCAAGAC AGATCACAAC TACTAGCCCC
    ACCAAACGTT CCCCCACCAA GCCTTCTGTT TCTCCCCTTT CAGTTACCCC CATGAAAAGT
    CCCCATGCAA CCCAGAAAAC AGGTGTCCCT TCATTCACAA AGCCTGTTCC ACCCACTCAG
    AAGCCAGCGC CCTTCACCTC CTACCTAGCA CCTTCCAAAG CCTCTAGTCC CACTGTGAGG
    CCTGTTCAGA AGACTTTCAT GACACCCCGA CCTCCAGTCC CCAGCCCCCA GCCCCTGCGC
    CCTACTACTG GCTTATCTAA GAAGTTCACT AACCCCACTG TTGCAAAATC TAAGTCCAAG
    ACGACCAGTT GGGCCAGCAA GCCGGTCTTG GCCCGCTCCA GTGTCCCTAA AACGCTTCAG
    CAAACTGTTT TGTCCCAGTC TCCTGTTTCC TATCTGGGCT CTCAGACACT GGCTCCAGCC
    CTCCCTCCTT TGGGTGTTGG AAATCCTAGA ACGATGCCTC CCACACGTGA CTCTGCTTTA
    ACTCCTGCTG GAAGCAAGAA ATTCACAGGA CGGGAAACCT CCAAGAAAAC CAGACAGAAG
    AGCAGCCCCC GGAAGCCCGA ACCTCTCAGC CCTGGGAAGT CAGCCAGGGA TGCCTCACCA
    AGAGACCTGA CAACCAAGCC TAGCCGGCCG TCCACCCCAG CCCTGGTCCT TGCCCCAGCT
    TACCTCCTGT CCTCCAGCCC CCAGCCCACC AGCAGCAGCT TCCCTTTCTT CCACCTGCTG
    GGCCCTACAC CTTTCCCGAT GCTGATGGGG CCTCCAGGCT CCAAGGGAGA CTGTGGCTTG
    CCGGGTCCCC CTGGGCTTCC AGGATTACCT GGATCTCCGG GCGCACGAGG TCCTCGGGGT
    CCTCCTGGGC CGTACGGGAA CCCAGGCCCA CCTGGCCCTC CAGGAGCCAA AGGACAGAAA
    GGGGACCCAG GACTCTCACC AGGACAGGCT CACGATGGAG CAAAGGGCAA CATGGGCTTG
    CCTGGGCTCT CCGGAAATCC AGGACCCTTG GGAAGGAAGG GACACAAGGG CCATCCTGGA
    GCAGCAGGGC ACCCTGGAGA ACAGGGGCAG CCAGGACCTG AGGGCAGTCC AGGGGCCAAA
    GGTTACCCTG GCAGGCAGGG GTTCCCTGGA CCTGTAGGAG ACCCTGGCCC CAAAGGCAGC
    AGGGGTTACA TTGGTCTTCC GGGGCTCTTT GGCCTGCCAG GGTCGGATGG AGAGCGGGGT
    CTCCCTGGCG TTCCTGGCAA GAGGGGCGAG ATGGGTAGGC CGGGCTTCCC TGGAGACTTT
    GGGGAAAGAG GCCCCCCTGG ACTTGATGGC AACCCTGGAG AAATTGGCCT GCCGGGGCCA
    CCAGGAGTGC TCGGGCTCAT TGGTGACACA GGAGCACTGG GCCCAGTTGG CTACCCAGGA
    CCGAAAGGCA TGAAGGGACT GATGGGCGGC GTGGGGGAGC CCGGACTGAA AGGTGATAAG
    GGTGAACAAG GGGTGCCAGG TGTGTCGGGA GATCCTGGCT TCCAAGGAGA CAAGGGGAGT
    CATGGCTTGC CAGGGCTCCC GGGTGGCCGG GGGAAGCCAG GGCCTCTGGG AAAAGCTGGA
    GACAAAGGAT CACTTGGGTT TCCTGGACCT CCTGGACCTG AGGGTTTCCC AGGAGACATT
    GGCCCTCCAG GGGACAATGG CCCTGAAGGC ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG
    CCTGGACCTC CTGGGCAGCT GGGGCCTGAG GGAGATGAAG GACCCATGGG GCCACCAGGA
    GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT
    GGCTCGAAGG GGGAACCAGG GGATCCTGGA CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT
    ATGGGATTCA TTGGTCTTGT CGGAGAGCCT GGAATTGTTG GGGAGAAGGG AGACCGTGGG
    GTGATGGGAC CCCCAGGTGC ACCTGGACCC AAGGGGTCGA TGGTAAGGAG GCCATCCTGG
    AACACCCGGT GGTATTGGGA ACCCTGGAGA GCCTGGACCC TGGGGCCCTC CAGGATCTCG
    AGGCCTGCCA GGCATGAGGG GAGCCAAGGG GCACCGGGGT CTCCAGCTGA CTTACACAGG
    CTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu33010
    Clone ID Related Accession (Same CDS sequence) XM_006538040.3
    Accession Version XM_006538040.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3174bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product collagen alpha-1(XXVII) chain isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006538040.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGGCACGG GATTCGCGCG GGGGGCCCGA GGCACAGCGG CGTCAGGACC CGGGGGGGGG 
    TTTCTCTTCG CCTGGATCTT GGTCTCATTT ACCTGTCACC TGGCCTCCAC CCAAGGAGCT
    CCTGAAGATG TGGATGTCCT CCAGCGGCTG GGCCTCAGCT GGACGAAGGC CGGGGGTGGC
    CGGAGTCCTA CACCCCCTGG TGTCATTCCT TTCCCATCTG GCTTCATCTT CACACAGCGG
    GCCAAGCTGC AGGCCCCCAC AGCCAATGTT CTTCCCACCA CCCTGGGGCG GGAGCTGGCA
    TTGGTGTTGA GCCTCTGTTC ACATCGTGTG AACCATGCCT TCCTCTTTGC CATCCGCAGC
    AGGAAACACA AGCTGCAACT GGGTCTGCAG TTCCTCCCCG GCAGGACGAT CATCCACCTG
    GGGCCTCGGC AATCAGTGGC CTTCGACTTG GATGTGCATG ATGGGCGCTG GCACCACCTG
    GCCCTAGAGT TGCGTGGCCG CACAGTCACA ATGGTGACAG CCTGTGGGCA GCACAGGGTA
    CCTGTCCCAC TGCCTTCCCG TAGGGACTCC ATGCTTGACC CCCAGGGCTC CTTTCTCTTG
    GGGAAGGTGA ACCCCCGTGC TGTCCAGTTT GAAGGTGCAC TCTGTCAGTT CAGCATCCAC
    CCTGTGGCGC AGGTCGCTCA CAATTACTGT GCCCACCTGA GAGAGCGGTG CCGACAGGTG
    GACACATATA GTCCCCAGGT GGGAACCCTC TTTCCTTGGG ACTCTGGCCC AGCCTTTGCT
    TTGCATCCTG AACCAGCCTT GCTTGGCTTG GGGAATCTGA CCAGAACTCC AGCAACCCTG
    GGGGCCAGGC CTGTAAGCAG GGCACTTGCA GTGACTCTGG CTCCTGCCAT GCCCACCAAG
    CCCCTGAGGA CTGTCCACCC AGATGTCTCT GAACACAGCT CTTCCCAGAC CCCACTGTCA
    CCTGCCAAAC AGTCGGCCAG GAAAACACCT TCCCCCTCCT CGTCAGCTTC CCTGGCTAAT
    TCCACCAGGG TTTATCGTCC AGCTGCTGCC CAGCCAAGAC AGATCACAAC TACTAGCCCC
    ACCAAACGTT CCCCCACCAA GCCTTCTGTT TCTCCCCTTT CAGTTACCCC CATGAAAAGT
    CCCCATGCAA CCCAGAAAAC AGGTGTCCCT TCATTCACAA AGCCTGTTCC ACCCACTCAG
    AAGCCAGCGC CCTTCACCTC CTACCTAGCA CCTTCCAAAG CCTCTAGTCC CACTGTGAGG
    CCTGTTCAGA AGACTTTCAT GACACCCCGA CCTCCAGTCC CCAGCCCCCA GCCCCTGCGC
    CCTACTACTG GCTTATCTAA GAAGTTCACT AACCCCACTG TTGCAAAATC TAAGTCCAAG
    ACGACCAGTT GGGCCAGCAA GCCGGTCTTG GCCCGCTCCA GTGTCCCTAA AACGCTTCAG
    CAAACTGTTT TGTCCCAGTC TCCTGTTTCC TATCTGGGCT CTCAGACACT GGCTCCAGCC
    CTCCCTCCTT TGGGTGTTGG AAATCCTAGA ACGATGCCTC CCACACGTGA CTCTGCTTTA
    ACTCCTGCTG GAAGCAAGAA ATTCACAGGA CGGGAAACCT CCAAGAAAAC CAGACAGAAG
    AGCAGCCCCC GGAAGCCCGA ACCTCTCAGC CCTGGGAAGT CAGCCAGGGA TGCCTCACCA
    AGAGACCTGA CAACCAAGCC TAGCCGGCCG TCCACCCCAG CCCTGGTCCT TGCCCCAGCT
    TACCTCCTGT CCTCCAGCCC CCAGCCCACC AGCAGCAGCT TCCCTTTCTT CCACCTGCTG
    GGCCCTACAC CTTTCCCGAT GCTGATGGGG CCTCCAGGCT CCAAGGGAGA CTGTGGCTTG
    CCGGGTCCCC CTGGGCTTCC AGGATTACCT GGATCTCCGG GCGCACGAGG TCCTCGGGGT
    CCTCCTGGGC CGTACGGGAA CCCAGGCCCA CCTGGCCCTC CAGGAGCCAA AGGACAGAAA
    GGGGACCCAG GACTCTCACC AGGACAGGCT CACGATGGAG CAAAGGGCAA CATGGGCTTG
    CCTGGGCTCT CCGGAAATCC AGGACCCTTG GGAAGGAAGG GACACAAGGG CCATCCTGGA
    GCAGCAGGGC ACCCTGGAGA ACAGGGGCAG CCAGGACCTG AGGGCAGTCC AGGGGCCAAA
    GGTTACCCTG GCAGGCAGGG GTTCCCTGGA CCTGTAGGAG ACCCTGGCCC CAAAGGCAGC
    AGGGGTTACA TTGGTCTTCC GGGGCTCTTT GGCCTGCCAG GGTCGGATGG AGAGCGGGGT
    CTCCCTGGCG TTCCTGGCAA GAGGGGCGAG ATGGGTAGGC CGGGCTTCCC TGGAGACTTT
    GGGGAAAGAG GCCCCCCTGG ACTTGATGGC AACCCTGGAG AAATTGGCCT GCCGGGGCCA
    CCAGGAGTGC TCGGGCTCAT TGGTGACACA GGAGCACTGG GCCCAGTTGG CTACCCAGGA
    CCGAAAGGCA TGAAGGGACT GATGGGCGGC GTGGGGGAGC CCGGACTGAA AGGTGATAAG
    GGTGAACAAG GGGTGCCAGG TGTGTCGGGA GATCCTGGCT TCCAAGGAGA CAAGGGGAGT
    CATGGCTTGC CAGGGCTCCC GGGTGGCCGG GGGAAGCCAG GGCCTCTGGG AAAAGCTGGA
    GACAAAGGAT CACTTGGGTT TCCTGGACCT CCTGGACCTG AGGGTTTCCC AGGAGACATT
    GGCCCTCCAG GGGACAATGG CCCTGAAGGC ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG
    CCTGGACCTC CTGGGCAGCT GGGGCCTGAG GGAGATGAAG GACCCATGGG GCCACCAGGA
    GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT
    GGCTCGAAGG GGGAACCAGG GGATCCTGGA CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT
    ATGGGATTCA TTGGTCTTGT CGGAGAGCCT GGAATTGTTG GGGAGAAGGG AGACCGTGGG
    GTGATGGGAC CCCCAGGTGC ACCTGGACCC AAGGGGTCGA TGGTAAGGAG GCCATCCTGG
    AACACCCGGT GGTATTGGGA ACCCTGGAGA GCCTGGACCC TGGGGCCCTC CAGGATCTCG
    AGGCCTGCCA GGCATGAGGG GAGCCAAGGG GCACCGGGGC CCTCGAGGAC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006538103.1
    CDS509..3682
    Translation

    Target ORF information:

    RefSeq Version XM_006538040.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006538040.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGGCACGG GATTCGCGCG GGGGGCCCGA GGCACAGCGG CGTCAGGACC CGGGGGGGGG 
    TTTCTCTTCG CCTGGATCTT GGTCTCATTT ACCTGTCACC TGGCCTCCAC CCAAGGAGCT
    CCTGAAGATG TGGATGTCCT CCAGCGGCTG GGCCTCAGCT GGACGAAGGC CGGGGGTGGC
    CGGAGTCCTA CACCCCCTGG TGTCATTCCT TTCCCATCTG GCTTCATCTT CACACAGCGG
    GCCAAGCTGC AGGCCCCCAC AGCCAATGTT CTTCCCACCA CCCTGGGGCG GGAGCTGGCA
    TTGGTGTTGA GCCTCTGTTC ACATCGTGTG AACCATGCCT TCCTCTTTGC CATCCGCAGC
    AGGAAACACA AGCTGCAACT GGGTCTGCAG TTCCTCCCCG GCAGGACGAT CATCCACCTG
    GGGCCTCGGC AATCAGTGGC CTTCGACTTG GATGTGCATG ATGGGCGCTG GCACCACCTG
    GCCCTAGAGT TGCGTGGCCG CACAGTCACA ATGGTGACAG CCTGTGGGCA GCACAGGGTA
    CCTGTCCCAC TGCCTTCCCG TAGGGACTCC ATGCTTGACC CCCAGGGCTC CTTTCTCTTG
    GGGAAGGTGA ACCCCCGTGC TGTCCAGTTT GAAGGTGCAC TCTGTCAGTT CAGCATCCAC
    CCTGTGGCGC AGGTCGCTCA CAATTACTGT GCCCACCTGA GAGAGCGGTG CCGACAGGTG
    GACACATATA GTCCCCAGGT GGGAACCCTC TTTCCTTGGG ACTCTGGCCC AGCCTTTGCT
    TTGCATCCTG AACCAGCCTT GCTTGGCTTG GGGAATCTGA CCAGAACTCC AGCAACCCTG
    GGGGCCAGGC CTGTAAGCAG GGCACTTGCA GTGACTCTGG CTCCTGCCAT GCCCACCAAG
    CCCCTGAGGA CTGTCCACCC AGATGTCTCT GAACACAGCT CTTCCCAGAC CCCACTGTCA
    CCTGCCAAAC AGTCGGCCAG GAAAACACCT TCCCCCTCCT CGTCAGCTTC CCTGGCTAAT
    TCCACCAGGG TTTATCGTCC AGCTGCTGCC CAGCCAAGAC AGATCACAAC TACTAGCCCC
    ACCAAACGTT CCCCCACCAA GCCTTCTGTT TCTCCCCTTT CAGTTACCCC CATGAAAAGT
    CCCCATGCAA CCCAGAAAAC AGGTGTCCCT TCATTCACAA AGCCTGTTCC ACCCACTCAG
    AAGCCAGCGC CCTTCACCTC CTACCTAGCA CCTTCCAAAG CCTCTAGTCC CACTGTGAGG
    CCTGTTCAGA AGACTTTCAT GACACCCCGA CCTCCAGTCC CCAGCCCCCA GCCCCTGCGC
    CCTACTACTG GCTTATCTAA GAAGTTCACT AACCCCACTG TTGCAAAATC TAAGTCCAAG
    ACGACCAGTT GGGCCAGCAA GCCGGTCTTG GCCCGCTCCA GTGTCCCTAA AACGCTTCAG
    CAAACTGTTT TGTCCCAGTC TCCTGTTTCC TATCTGGGCT CTCAGACACT GGCTCCAGCC
    CTCCCTCCTT TGGGTGTTGG AAATCCTAGA ACGATGCCTC CCACACGTGA CTCTGCTTTA
    ACTCCTGCTG GAAGCAAGAA ATTCACAGGA CGGGAAACCT CCAAGAAAAC CAGACAGAAG
    AGCAGCCCCC GGAAGCCCGA ACCTCTCAGC CCTGGGAAGT CAGCCAGGGA TGCCTCACCA
    AGAGACCTGA CAACCAAGCC TAGCCGGCCG TCCACCCCAG CCCTGGTCCT TGCCCCAGCT
    TACCTCCTGT CCTCCAGCCC CCAGCCCACC AGCAGCAGCT TCCCTTTCTT CCACCTGCTG
    GGCCCTACAC CTTTCCCGAT GCTGATGGGG CCTCCAGGCT CCAAGGGAGA CTGTGGCTTG
    CCGGGTCCCC CTGGGCTTCC AGGATTACCT GGATCTCCGG GCGCACGAGG TCCTCGGGGT
    CCTCCTGGGC CGTACGGGAA CCCAGGCCCA CCTGGCCCTC CAGGAGCCAA AGGACAGAAA
    GGGGACCCAG GACTCTCACC AGGACAGGCT CACGATGGAG CAAAGGGCAA CATGGGCTTG
    CCTGGGCTCT CCGGAAATCC AGGACCCTTG GGAAGGAAGG GACACAAGGG CCATCCTGGA
    GCAGCAGGGC ACCCTGGAGA ACAGGGGCAG CCAGGACCTG AGGGCAGTCC AGGGGCCAAA
    GGTTACCCTG GCAGGCAGGG GTTCCCTGGA CCTGTAGGAG ACCCTGGCCC CAAAGGCAGC
    AGGGGTTACA TTGGTCTTCC GGGGCTCTTT GGCCTGCCAG GGTCGGATGG AGAGCGGGGT
    CTCCCTGGCG TTCCTGGCAA GAGGGGCGAG ATGGGTAGGC CGGGCTTCCC TGGAGACTTT
    GGGGAAAGAG GCCCCCCTGG ACTTGATGGC AACCCTGGAG AAATTGGCCT GCCGGGGCCA
    CCAGGAGTGC TCGGGCTCAT TGGTGACACA GGAGCACTGG GCCCAGTTGG CTACCCAGGA
    CCGAAAGGCA TGAAGGGACT GATGGGCGGC GTGGGGGAGC CCGGACTGAA AGGTGATAAG
    GGTGAACAAG GGGTGCCAGG TGTGTCGGGA GATCCTGGCT TCCAAGGAGA CAAGGGGAGT
    CATGGCTTGC CAGGGCTCCC GGGTGGCCGG GGGAAGCCAG GGCCTCTGGG AAAAGCTGGA
    GACAAAGGAT CACTTGGGTT TCCTGGACCT CCTGGACCTG AGGGTTTCCC AGGAGACATT
    GGCCCTCCAG GGGACAATGG CCCTGAAGGC ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG
    CCTGGACCTC CTGGGCAGCT GGGGCCTGAG GGAGATGAAG GACCCATGGG GCCACCAGGA
    GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT
    GGCTCGAAGG GGGAACCAGG GGATCCTGGA CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT
    ATGGGATTCA TTGGTCTTGT CGGAGAGCCT GGAATTGTTG GGGAGAAGGG AGACCGTGGG
    GTGATGGGAC CCCCAGGTGC ACCTGGACCC AAGGGGTCGA TGGTAAGGAG GCCATCCTGG
    AACACCCGGT GGTATTGGGA ACCCTGGAGA GCCTGGACCC TGGGGCCCTC CAGGATCTCG
    AGGCCTGCCA GGCATGAGGG GAGCCAAGGG GCACCGGGGC CCTCGAGGAC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu74976
    Clone ID Related Accession (Same CDS sequence) XM_011250057.3 , XM_011250057.2
    Accession Version XM_011250057.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3708bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product collagen alpha-1(XXVII) chain isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011250057.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGGCACGG GATTCGCGCG GGGGGCCCGA GGCACAGCGG CGTCAGGACC CGGGGGGGGG 
    TTTCTCTTCG CCTGGATCTT GGTCTCATTT ACCTGTCACC TGGCCTCCAC CCAAGGAGCT
    CCTGAAGATG TGGATGTCCT CCAGCGGCTG GGCCTCAGCT GGACGAAGGC CGGGGGTGGC
    CGGAGTCCTA CACCCCCTGG TGTCATTCCT TTCCCATCTG GCTTCATCTT CACACAGCGG
    GCCAAGCTGC AGGCCCCCAC AGCCAATGTT CTTCCCACCA CCCTGGGGCG GGAGCTGGCA
    TTGGTGTTGA GCCTCTGTTC ACATCGTGTG AACCATGCCT TCCTCTTTGC CATCCGCAGC
    AGGAAACACA AGCTGCAACT GGGTCTGCAG TTCCTCCCCG GCAGGACGAT CATCCACCTG
    GGGCCTCGGC AATCAGTGGC CTTCGACTTG GATGTGCATG ATGGGCGCTG GCACCACCTG
    GCCCTAGAGT TGCGTGGCCG CACAGTCACA ATGGTGACAG CCTGTGGGCA GCACAGGGTA
    CCTGTCCCAC TGCCTTCCCG TAGGGACTCC ATGCTTGACC CCCAGGGCTC CTTTCTCTTG
    GGGAAGGTGA ACCCCCGTGC TGTCCAGTTT GAAGGTGCAC TCTGTCAGTT CAGCATCCAC
    CCTGTGGCGC AGGTCGCTCA CAATTACTGT GCCCACCTGA GAGAGCGGTG CCGACAGGTG
    GACACATATA GTCCCCAGGT GGGAACCCTC TTTCCTTGGG ACTCTGGCCC AGCCTTTGCT
    TTGCATCCTG AACCAGCCTT GCTTGGCTTG GGGAATCTGA CCAGAACTCC AGCAACCCTG
    GGGGCCAGGC CTGTAAGCAG GGCACTTGCA GTGACTCTGG CTCCTGCCAT GCCCACCAAG
    CCCCTGAGGA CTGTCCACCC AGATGTCTCT GAACACAGCT CTTCCCAGAC CCCACTGTCA
    CCTGCCAAAC AGTCGGCCAG GAAAACACCT TCCCCCTCCT CGTCAGCTTC CCTGGCTAAT
    TCCACCAGGG TTTATCGTCC AGCTGCTGCC CAGCCAAGAC AGATCACAAC TACTAGCCCC
    ACCAAACGTT CCCCCACCAA GCCTTCTGTT TCTCCCCTTT CAGTTACCCC CATGAAAAGT
    CCCCATGCAA CCCAGAAAAC AGGTGTCCCT TCATTCACAA AGCCTGTTCC ACCCACTCAG
    AAGCCAGCGC CCTTCACCTC CTACCTAGCA CCTTCCAAAG CCTCTAGTCC CACTGTGAGG
    CCTGTTCAGA AGACTTTCAT GACACCCCGA CCTCCAGTCC CCAGCCCCCA GCCCCTGCGC
    CCTACTACTG GCTTATCTAA GAAGTTCACT AACCCCACTG TTGCAAAATC TAAGTCCAAG
    ACGACCAGTT GGGCCAGCAA GCCGGTCTTG GCCCGCTCCA GTGTCCCTAA AACGCTTCAG
    CAAACTGTTT TGTCCCAGTC TCCTGTTTCC TATCTGGGCT CTCAGACACT GGCTCCAGCC
    CTCCCTCCTT TGGGTGTTGG AAATCCTAGA ACGATGCCTC CCACACGTGA CTCTGCTTTA
    ACTCCTGCTG GAAGCAAGAA ATTCACAGGA CGGGAAACCT CCAAGAAAAC CAGACAGAAG
    AGCAGCCCCC GGAAGCCCGA ACCTCTCAGC CCTGGGAAGT CAGCCAGGGA TGCCTCACCA
    AGAGACCTGA CAACCAAGCC TAGCCGGCCG TCCACCCCAG CCCTGGTCCT TGCCCCAGCT
    TACCTCCTGT CCTCCAGCCC CCAGCCCACC AGCAGCAGCT TCCCTTTCTT CCACCTGCTG
    GGCCCTACAC CTTTCCCGAT GCTGATGGGG CCTCCAGGCT CCAAGGGAGA CTGTGGCTTG
    CCGGGTCCCC CTGGGCTTCC AGGATTACCT GGATCTCCGG GCGCACGAGG TCCTCGGGGT
    CCTCCTGGGC CGTACGGGAA CCCAGGCCCA CCTGGCCCTC CAGGAGCCAA AGGACAGAAA
    GGGGACCCAG GACTCTCACC AGGACAGGCT CACGATGGAG CAAAGGGCAA CATGGGCTTG
    CCTGGGCTCT CCGGAAATCC AGGACCCTTG GGAAGGAAGG GACACAAGGG CCATCCTGGA
    GCAGCAGGGC ACCCTGGAGA ACAGGGGCAG CCAGGACCTG AGGGCAGTCC AGGGGCCAAA
    GGTTACCCTG GCAGGCAGGG GTTCCCTGGA CCTGTAGGAG ACCCTGGCCC CAAAGGCAGC
    AGGGGTTACA TTGGTCTTCC GGGGCTCTTT GGCCTGCCAG GGTCGGATGG AGAGCGGGGT
    CTCCCTGGCG TTCCTGGCAA GAGGGGCGAG ATGGGTAGGC CGGGCTTCCC TGGAGACTTT
    GGGGAAAGAG GCCCCCCTGG ACTTGATGGC AACCCTGGAG AAATTGGCCT GCCGGGGCCA
    CCAGGAGTGC TCGGGCTCAT TGGTGACACA GGAGCACTGG GCCCAGTTGG CTACCCAGGA
    CCGAAAGGCA TGAAGGGACT GATGGGCGGC GTGGGGGAGC CCGGACTGAA AGGTGATAAG
    GGTGAACAAG GGGTGCCAGG TGTGTCGGGA GATCCTGGCT TCCAAGGAGA CAAGGGGAGT
    CATGGCTTGC CAGGGCTCCC GGGTGGCCGG GGGAAGCCAG GGCCTCTGGG AAAAGCTGGA
    GACAAAGGAT CACTTGGGTT TCCTGGACCT CCTGGACCTG AGGGTTTCCC AGGAGACATT
    GGCCCTCCAG GGGACAATGG CCCTGAAGGC ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG
    CCTGGACCTC CTGGGCAGCT GGGGCCTGAG GGAGATGAAG GACCCATGGG GCCACCAGGA
    GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT
    GGCTCGAAGG GGGAACCAGG GGATCCTGGA CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT
    ATGGGATTCA TTGGTCTTGT CGGAGAGCCT GGAATTGTTG GGGAGAAGGG AGACCGTGGG
    GTGATGGGAC CCCCAGGTGC ACCTGGACCC AAGGGGTCGA TGGGCCATCC TGGAACACCC
    GGTGGTATTG GGAACCCTGG AGAGCCTGGA CCCTGGGGCC CTCCAGGATC TCGAGGCCTG
    CCAGGCATGA GGGGAGCCAA GGGGCACCGG GGCCCTCGAG GACCTGATGG ACCAGCTGGG
    GAGCAGGGAT CCAAGGGCCT GAAGGGTCGT GTAGGACCTC GGGGCAGACC TGGCCAGCCA
    GGACAGCAGG GTGCAGCTGG TGAGCGAGGC CACTCAGGTG CAAAAGGCTT CCTTGGCATC
    CCTGGTCCCT CAGGCCCTCC AGGTGCCAAG GGCCTCCCAG GAGAACCGGG CTCTCAGGGA
    CCTCAGGGGC CAGTTGGTCC TCCAGGAGAA ATGGGGCCCA AGGGACCGCC GGGTGCAGTG
    GGAGAGCCTG GCCTTCCTGG GGACTCCGGA ATGAAGGGTG ACCTCGGACC TCTGGGCCCC
    CCTGGGGAGC AAGGTCTTAT TGGGCAGCGG GGAGAGCCAG GCCTTGAGGG AGACCATGGC
    CCTGTGGGGC CAGATGGGCT GAAGGGCGAC AGGGGCGACC CAGGGCCCGA TGGCGAGCAT
    GGTGAAAAGG GCCAGGAAGG GCTGAAAGGA GAGGATGGGT CCCCTGGGCC CCCGGGCATC
    ACTGGCGTCC CGATCCATCC ACAGACACTC CAGCTCAGAT GGTCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011248359.1
    CDS510..4217
    Translation

    Target ORF information:

    RefSeq Version XM_011250057.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011250057.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGGCACGG GATTCGCGCG GGGGGCCCGA GGCACAGCGG CGTCAGGACC CGGGGGGGGG 
    TTTCTCTTCG CCTGGATCTT GGTCTCATTT ACCTGTCACC TGGCCTCCAC CCAAGGAGCT
    CCTGAAGATG TGGATGTCCT CCAGCGGCTG GGCCTCAGCT GGACGAAGGC CGGGGGTGGC
    CGGAGTCCTA CACCCCCTGG TGTCATTCCT TTCCCATCTG GCTTCATCTT CACACAGCGG
    GCCAAGCTGC AGGCCCCCAC AGCCAATGTT CTTCCCACCA CCCTGGGGCG GGAGCTGGCA
    TTGGTGTTGA GCCTCTGTTC ACATCGTGTG AACCATGCCT TCCTCTTTGC CATCCGCAGC
    AGGAAACACA AGCTGCAACT GGGTCTGCAG TTCCTCCCCG GCAGGACGAT CATCCACCTG
    GGGCCTCGGC AATCAGTGGC CTTCGACTTG GATGTGCATG ATGGGCGCTG GCACCACCTG
    GCCCTAGAGT TGCGTGGCCG CACAGTCACA ATGGTGACAG CCTGTGGGCA GCACAGGGTA
    CCTGTCCCAC TGCCTTCCCG TAGGGACTCC ATGCTTGACC CCCAGGGCTC CTTTCTCTTG
    GGGAAGGTGA ACCCCCGTGC TGTCCAGTTT GAAGGTGCAC TCTGTCAGTT CAGCATCCAC
    CCTGTGGCGC AGGTCGCTCA CAATTACTGT GCCCACCTGA GAGAGCGGTG CCGACAGGTG
    GACACATATA GTCCCCAGGT GGGAACCCTC TTTCCTTGGG ACTCTGGCCC AGCCTTTGCT
    TTGCATCCTG AACCAGCCTT GCTTGGCTTG GGGAATCTGA CCAGAACTCC AGCAACCCTG
    GGGGCCAGGC CTGTAAGCAG GGCACTTGCA GTGACTCTGG CTCCTGCCAT GCCCACCAAG
    CCCCTGAGGA CTGTCCACCC AGATGTCTCT GAACACAGCT CTTCCCAGAC CCCACTGTCA
    CCTGCCAAAC AGTCGGCCAG GAAAACACCT TCCCCCTCCT CGTCAGCTTC CCTGGCTAAT
    TCCACCAGGG TTTATCGTCC AGCTGCTGCC CAGCCAAGAC AGATCACAAC TACTAGCCCC
    ACCAAACGTT CCCCCACCAA GCCTTCTGTT TCTCCCCTTT CAGTTACCCC CATGAAAAGT
    CCCCATGCAA CCCAGAAAAC AGGTGTCCCT TCATTCACAA AGCCTGTTCC ACCCACTCAG
    AAGCCAGCGC CCTTCACCTC CTACCTAGCA CCTTCCAAAG CCTCTAGTCC CACTGTGAGG
    CCTGTTCAGA AGACTTTCAT GACACCCCGA CCTCCAGTCC CCAGCCCCCA GCCCCTGCGC
    CCTACTACTG GCTTATCTAA GAAGTTCACT AACCCCACTG TTGCAAAATC TAAGTCCAAG
    ACGACCAGTT GGGCCAGCAA GCCGGTCTTG GCCCGCTCCA GTGTCCCTAA AACGCTTCAG
    CAAACTGTTT TGTCCCAGTC TCCTGTTTCC TATCTGGGCT CTCAGACACT GGCTCCAGCC
    CTCCCTCCTT TGGGTGTTGG AAATCCTAGA ACGATGCCTC CCACACGTGA CTCTGCTTTA
    ACTCCTGCTG GAAGCAAGAA ATTCACAGGA CGGGAAACCT CCAAGAAAAC CAGACAGAAG
    AGCAGCCCCC GGAAGCCCGA ACCTCTCAGC CCTGGGAAGT CAGCCAGGGA TGCCTCACCA
    AGAGACCTGA CAACCAAGCC TAGCCGGCCG TCCACCCCAG CCCTGGTCCT TGCCCCAGCT
    TACCTCCTGT CCTCCAGCCC CCAGCCCACC AGCAGCAGCT TCCCTTTCTT CCACCTGCTG
    GGCCCTACAC CTTTCCCGAT GCTGATGGGG CCTCCAGGCT CCAAGGGAGA CTGTGGCTTG
    CCGGGTCCCC CTGGGCTTCC AGGATTACCT GGATCTCCGG GCGCACGAGG TCCTCGGGGT
    CCTCCTGGGC CGTACGGGAA CCCAGGCCCA CCTGGCCCTC CAGGAGCCAA AGGACAGAAA
    GGGGACCCAG GACTCTCACC AGGACAGGCT CACGATGGAG CAAAGGGCAA CATGGGCTTG
    CCTGGGCTCT CCGGAAATCC AGGACCCTTG GGAAGGAAGG GACACAAGGG CCATCCTGGA
    GCAGCAGGGC ACCCTGGAGA ACAGGGGCAG CCAGGACCTG AGGGCAGTCC AGGGGCCAAA
    GGTTACCCTG GCAGGCAGGG GTTCCCTGGA CCTGTAGGAG ACCCTGGCCC CAAAGGCAGC
    AGGGGTTACA TTGGTCTTCC GGGGCTCTTT GGCCTGCCAG GGTCGGATGG AGAGCGGGGT
    CTCCCTGGCG TTCCTGGCAA GAGGGGCGAG ATGGGTAGGC CGGGCTTCCC TGGAGACTTT
    GGGGAAAGAG GCCCCCCTGG ACTTGATGGC AACCCTGGAG AAATTGGCCT GCCGGGGCCA
    CCAGGAGTGC TCGGGCTCAT TGGTGACACA GGAGCACTGG GCCCAGTTGG CTACCCAGGA
    CCGAAAGGCA TGAAGGGACT GATGGGCGGC GTGGGGGAGC CCGGACTGAA AGGTGATAAG
    GGTGAACAAG GGGTGCCAGG TGTGTCGGGA GATCCTGGCT TCCAAGGAGA CAAGGGGAGT
    CATGGCTTGC CAGGGCTCCC GGGTGGCCGG GGGAAGCCAG GGCCTCTGGG AAAAGCTGGA
    GACAAAGGAT CACTTGGGTT TCCTGGACCT CCTGGACCTG AGGGTTTCCC AGGAGACATT
    GGCCCTCCAG GGGACAATGG CCCTGAAGGC ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG
    CCTGGACCTC CTGGGCAGCT GGGGCCTGAG GGAGATGAAG GACCCATGGG GCCACCAGGA
    GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT
    GGCTCGAAGG GGGAACCAGG GGATCCTGGA CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT
    ATGGGATTCA TTGGTCTTGT CGGAGAGCCT GGAATTGTTG GGGAGAAGGG AGACCGTGGG
    GTGATGGGAC CCCCAGGTGC ACCTGGACCC AAGGGGTCGA TGGGCCATCC TGGAACACCC
    GGTGGTATTG GGAACCCTGG AGAGCCTGGA CCCTGGGGCC CTCCAGGATC TCGAGGCCTG
    CCAGGCATGA GGGGAGCCAA GGGGCACCGG GGCCCTCGAG GACCTGATGG ACCAGCTGGG
    GAGCAGGGAT CCAAGGGCCT GAAGGGTCGT GTAGGACCTC GGGGCAGACC TGGCCAGCCA
    GGACAGCAGG GTGCAGCTGG TGAGCGAGGC CACTCAGGTG CAAAAGGCTT CCTTGGCATC
    CCTGGTCCCT CAGGCCCTCC AGGTGCCAAG GGCCTCCCAG GAGAACCGGG CTCTCAGGGA
    CCTCAGGGGC CAGTTGGTCC TCCAGGAGAA ATGGGGCCCA AGGGACCGCC GGGTGCAGTG
    GGAGAGCCTG GCCTTCCTGG GGACTCCGGA ATGAAGGGTG ACCTCGGACC TCTGGGCCCC
    CCTGGGGAGC AAGGTCTTAT TGGGCAGCGG GGAGAGCCAG GCCTTGAGGG AGACCATGGC
    CCTGTGGGGC CAGATGGGCT GAAGGGCGAC AGGGGCGACC CAGGGCCCGA TGGCGAGCAT
    GGTGAAAAGG GCCAGGAAGG GCTGAAAGGA GAGGATGGGT CCCCTGGGCC CCCGGGCATC
    ACTGGCGTCC CGATCCATCC ACAGACACTC CAGCTCAGAT GGTCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu74977
    Clone ID Related Accession (Same CDS sequence) XM_011250058.3 , XM_011250058.2
    Accession Version XM_011250058.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3186bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product collagen alpha-1(XXVII) chain isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011250058.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGGCACGG GATTCGCGCG GGGGGCCCGA GGCACAGCGG CGTCAGGACC CGGGGGGGGG 
    TTTCTCTTCG CCTGGATCTT GGTCTCATTT ACCTGTCACC TGGCCTCCAC CCAAGGAGCT
    CCTGAAGATG TGGATGTCCT CCAGCGGCTG GGCCTCAGCT GGACGAAGGC CGGGGGTGGC
    CGGAGTCCTA CACCCCCTGG TGTCATTCCT TTCCCATCTG GCTTCATCTT CACACAGCGG
    GCCAAGCTGC AGGCCCCCAC AGCCAATGTT CTTCCCACCA CCCTGGGGCG GGAGCTGGCA
    TTGGTGTTGA GCCTCTGTTC ACATCGTGTG AACCATGCCT TCCTCTTTGC CATCCGCAGC
    AGGAAACACA AGCTGCAACT GGGTCTGCAG TTCCTCCCCG GCAGGACGAT CATCCACCTG
    GGGCCTCGGC AATCAGTGGC CTTCGACTTG GATGTGCATG ATGGGCGCTG GCACCACCTG
    GCCCTAGAGT TGCGTGGCCG CACAGTCACA ATGGTGACAG CCTGTGGGCA GCACAGGGTA
    CCTGTCCCAC TGCCTTCCCG TAGGGACTCC ATGCTTGACC CCCAGGGCTC CTTTCTCTTG
    GGGAAGGTGA ACCCCCGTGC TGTCCAGTTT GAAGGTGCAC TCTGTCAGTT CAGCATCCAC
    CCTGTGGCGC AGGTCGCTCA CAATTACTGT GCCCACCTGA GAGAGCGGTG CCGACAGGTG
    GACACATATA GTCCCCAGGT GGGAACCCTC TTTCCTTGGG ACTCTGGCCC AGCCTTTGCT
    TTGCATCCTG AACCAGCCTT GCTTGGCTTG GGGAATCTGA CCAGAACTCC AGCAACCCTG
    GGGGCCAGGC CTGTAAGCAG GGCACTTGCA GTGACTCTGG CTCCTGCCAT GCCCACCAAG
    CCCCTGAGGA CTGTCCACCC AGATGTCTCT GAACACAGCT CTTCCCAGAC CCCACTGTCA
    CCTGCCAAAC AGTCGGCCAG GAAAACACCT TCCCCCTCCT CGTCAGCTTC CCTGGCTAAT
    TCCACCAGGG TTTATCGTCC AGCTGCTGCC CAGCCAAGAC AGATCACAAC TACTAGCCCC
    ACCAAACGTT CCCCCACCAA GCCTTCTGTT TCTCCCCTTT CAGTTACCCC CATGAAAAGT
    CCCCATGCAA CCCAGAAAAC AGGTGTCCCT TCATTCACAA AGCCTGTTCC ACCCACTCAG
    AAGCCAGCGC CCTTCACCTC CTACCTAGCA CCTTCCAAAG CCTCTAGTCC CACTGTGAGG
    CCTGTTCAGA AGACTTTCAT GACACCCCGA CCTCCAGTCC CCAGCCCCCA GCCCCTGCGC
    CCTACTACTG GCTTATCTAA GAAGTTCACT AACCCCACTG TTGCAAAATC TAAGTCCAAG
    ACGACCAGTT GGGCCAGCAA GCCGGTCTTG GCCCGCTCCA GTGTCCCTAA AACGCTTCAG
    CAAACTGTTT TGTCCCAGTC TCCTGTTTCC TATCTGGGCT CTCAGACACT GGCTCCAGCC
    CTCCCTCCTT TGGGTGTTGG AAATCCTAGA ACGATGCCTC CCACACGTGA CTCTGCTTTA
    ACTCCTGCTG GAAGCAAGAA ATTCACAGGA CGGGAAACCT CCAAGAAAAC CAGACAGAAG
    AGCAGCCCCC GGAAGCCCGA ACCTCTCAGC CCTGGGAAGT CAGCCAGGGA TGCCTCACCA
    AGAGACCTGA CAACCAAGCC TAGCCGGCCG TCCACCCCAG CCCTGGTCCT TGCCCCAGCT
    TACCTCCTGT CCTCCAGCCC CCAGCCCACC AGCAGCAGCT TCCCTTTCTT CCACCTGCTG
    GGCCCTACAC CTTTCCCGAT GCTGATGGGG CCTCCAGGCT CCAAGGGAGA CTGTGGCTTG
    CCGGGTCCCC CTGGGCTTCC AGGATTACCT GGATCTCCGG GCGCACGAGG TCCTCGGGGT
    CCTCCTGGGC CGTACGGGAA CCCAGGCCCA CCTGGCCCTC CAGGAGCCAA AGGACAGAAA
    GGGGACCCAG GACTCTCACC AGGACAGGCT CACGATGGAG CAAAGGGCAA CATGGGCTTG
    CCTGGGCTCT CCGGAAATCC AGGACCCTTG GGAAGGAAGG GACACAAGGG CCATCCTGGA
    GCAGCAGGGC ACCCTGGAGA ACAGGGGCAG CCAGGACCTG AGGGCAGTCC AGGGGCCAAA
    GGTTACCCTG GCAGGCAGGG GTTCCCTGGA CCTGTAGGAG ACCCTGGCCC CAAAGGCAGC
    AGGGGTTACA TTGGTCTTCC GGGGCTCTTT GGCCTGCCAG GGTCGGATGG AGAGCGGGGT
    CTCCCTGGCG TTCCTGGCAA GAGGGGCGAG ATGGGTAGGC CGGGCTTCCC TGGAGACTTT
    GGGGAAAGAG GCCCCCCTGG ACTTGATGGC AACCCTGGAG AAATTGGCCT GCCGGGGCCA
    CCAGGAGTGC TCGGGCTCAT TGGTGACACA GGAGCACTGG GCCCAGTTGG CTACCCAGGA
    CCGAAAGGCA TGAAGGGACT GATGGGCGGC GTGGGGGAGC CCGGACTGAA AGGTGATAAG
    GGTGAACAAG GGGTGCCAGG TGTGTCGGGA GATCCTGGCT TCCAAGGAGA CAAGGGGAGT
    CATGGCTTGC CAGGGCTCCC GGGTGGCCGG GGGAAGCCAG GGCCTCTGGG AAAAGCTGGA
    GACAAAGGAT CACTTGGGTT TCCTGGACCT CCTGGACCTG AGGGTTTCCC AGGAGACATT
    GGCCCTCCAG GGGACAATGG CCCTGAAGGC ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG
    CCTGGACCTC CTGGGCAGCT GGGGCCTGAG GGAGATGAAG GACCCATGGG GCCACCAGGA
    GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT
    GGCTCGAAGG GGGAACCAGG GGATCCTGGA CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT
    ATGGGATTCA TTGGTCTTGT CGGAGAGCCT GGAATTGTTG GGGAGAAGGG AGACCGTGGG
    GTGATGGGAC CCCCAGGTGC ACCTGGACCC AAGGGGTCGA TGGGCCATCC TGGAACACCC
    GGTGGTATTG GGAACCCTGG AGAGCCTGGA CCCTGGGGCC CTCCAGGATC TCGAGGCCTG
    CCAGGCATGA GGGGAGCCAA GGGGCACCGG GGTCTCCAGC TGACTTACAC AGGCTGTGAC
    CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011248360.1
    CDS509..3694
    Translation

    Target ORF information:

    RefSeq Version XM_011250058.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011250058.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGGCACGG GATTCGCGCG GGGGGCCCGA GGCACAGCGG CGTCAGGACC CGGGGGGGGG 
    TTTCTCTTCG CCTGGATCTT GGTCTCATTT ACCTGTCACC TGGCCTCCAC CCAAGGAGCT
    CCTGAAGATG TGGATGTCCT CCAGCGGCTG GGCCTCAGCT GGACGAAGGC CGGGGGTGGC
    CGGAGTCCTA CACCCCCTGG TGTCATTCCT TTCCCATCTG GCTTCATCTT CACACAGCGG
    GCCAAGCTGC AGGCCCCCAC AGCCAATGTT CTTCCCACCA CCCTGGGGCG GGAGCTGGCA
    TTGGTGTTGA GCCTCTGTTC ACATCGTGTG AACCATGCCT TCCTCTTTGC CATCCGCAGC
    AGGAAACACA AGCTGCAACT GGGTCTGCAG TTCCTCCCCG GCAGGACGAT CATCCACCTG
    GGGCCTCGGC AATCAGTGGC CTTCGACTTG GATGTGCATG ATGGGCGCTG GCACCACCTG
    GCCCTAGAGT TGCGTGGCCG CACAGTCACA ATGGTGACAG CCTGTGGGCA GCACAGGGTA
    CCTGTCCCAC TGCCTTCCCG TAGGGACTCC ATGCTTGACC CCCAGGGCTC CTTTCTCTTG
    GGGAAGGTGA ACCCCCGTGC TGTCCAGTTT GAAGGTGCAC TCTGTCAGTT CAGCATCCAC
    CCTGTGGCGC AGGTCGCTCA CAATTACTGT GCCCACCTGA GAGAGCGGTG CCGACAGGTG
    GACACATATA GTCCCCAGGT GGGAACCCTC TTTCCTTGGG ACTCTGGCCC AGCCTTTGCT
    TTGCATCCTG AACCAGCCTT GCTTGGCTTG GGGAATCTGA CCAGAACTCC AGCAACCCTG
    GGGGCCAGGC CTGTAAGCAG GGCACTTGCA GTGACTCTGG CTCCTGCCAT GCCCACCAAG
    CCCCTGAGGA CTGTCCACCC AGATGTCTCT GAACACAGCT CTTCCCAGAC CCCACTGTCA
    CCTGCCAAAC AGTCGGCCAG GAAAACACCT TCCCCCTCCT CGTCAGCTTC CCTGGCTAAT
    TCCACCAGGG TTTATCGTCC AGCTGCTGCC CAGCCAAGAC AGATCACAAC TACTAGCCCC
    ACCAAACGTT CCCCCACCAA GCCTTCTGTT TCTCCCCTTT CAGTTACCCC CATGAAAAGT
    CCCCATGCAA CCCAGAAAAC AGGTGTCCCT TCATTCACAA AGCCTGTTCC ACCCACTCAG
    AAGCCAGCGC CCTTCACCTC CTACCTAGCA CCTTCCAAAG CCTCTAGTCC CACTGTGAGG
    CCTGTTCAGA AGACTTTCAT GACACCCCGA CCTCCAGTCC CCAGCCCCCA GCCCCTGCGC
    CCTACTACTG GCTTATCTAA GAAGTTCACT AACCCCACTG TTGCAAAATC TAAGTCCAAG
    ACGACCAGTT GGGCCAGCAA GCCGGTCTTG GCCCGCTCCA GTGTCCCTAA AACGCTTCAG
    CAAACTGTTT TGTCCCAGTC TCCTGTTTCC TATCTGGGCT CTCAGACACT GGCTCCAGCC
    CTCCCTCCTT TGGGTGTTGG AAATCCTAGA ACGATGCCTC CCACACGTGA CTCTGCTTTA
    ACTCCTGCTG GAAGCAAGAA ATTCACAGGA CGGGAAACCT CCAAGAAAAC CAGACAGAAG
    AGCAGCCCCC GGAAGCCCGA ACCTCTCAGC CCTGGGAAGT CAGCCAGGGA TGCCTCACCA
    AGAGACCTGA CAACCAAGCC TAGCCGGCCG TCCACCCCAG CCCTGGTCCT TGCCCCAGCT
    TACCTCCTGT CCTCCAGCCC CCAGCCCACC AGCAGCAGCT TCCCTTTCTT CCACCTGCTG
    GGCCCTACAC CTTTCCCGAT GCTGATGGGG CCTCCAGGCT CCAAGGGAGA CTGTGGCTTG
    CCGGGTCCCC CTGGGCTTCC AGGATTACCT GGATCTCCGG GCGCACGAGG TCCTCGGGGT
    CCTCCTGGGC CGTACGGGAA CCCAGGCCCA CCTGGCCCTC CAGGAGCCAA AGGACAGAAA
    GGGGACCCAG GACTCTCACC AGGACAGGCT CACGATGGAG CAAAGGGCAA CATGGGCTTG
    CCTGGGCTCT CCGGAAATCC AGGACCCTTG GGAAGGAAGG GACACAAGGG CCATCCTGGA
    GCAGCAGGGC ACCCTGGAGA ACAGGGGCAG CCAGGACCTG AGGGCAGTCC AGGGGCCAAA
    GGTTACCCTG GCAGGCAGGG GTTCCCTGGA CCTGTAGGAG ACCCTGGCCC CAAAGGCAGC
    AGGGGTTACA TTGGTCTTCC GGGGCTCTTT GGCCTGCCAG GGTCGGATGG AGAGCGGGGT
    CTCCCTGGCG TTCCTGGCAA GAGGGGCGAG ATGGGTAGGC CGGGCTTCCC TGGAGACTTT
    GGGGAAAGAG GCCCCCCTGG ACTTGATGGC AACCCTGGAG AAATTGGCCT GCCGGGGCCA
    CCAGGAGTGC TCGGGCTCAT TGGTGACACA GGAGCACTGG GCCCAGTTGG CTACCCAGGA
    CCGAAAGGCA TGAAGGGACT GATGGGCGGC GTGGGGGAGC CCGGACTGAA AGGTGATAAG
    GGTGAACAAG GGGTGCCAGG TGTGTCGGGA GATCCTGGCT TCCAAGGAGA CAAGGGGAGT
    CATGGCTTGC CAGGGCTCCC GGGTGGCCGG GGGAAGCCAG GGCCTCTGGG AAAAGCTGGA
    GACAAAGGAT CACTTGGGTT TCCTGGACCT CCTGGACCTG AGGGTTTCCC AGGAGACATT
    GGCCCTCCAG GGGACAATGG CCCTGAAGGC ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG
    CCTGGACCTC CTGGGCAGCT GGGGCCTGAG GGAGATGAAG GACCCATGGG GCCACCAGGA
    GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT
    GGCTCGAAGG GGGAACCAGG GGATCCTGGA CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT
    ATGGGATTCA TTGGTCTTGT CGGAGAGCCT GGAATTGTTG GGGAGAAGGG AGACCGTGGG
    GTGATGGGAC CCCCAGGTGC ACCTGGACCC AAGGGGTCGA TGGGCCATCC TGGAACACCC
    GGTGGTATTG GGAACCCTGG AGAGCCTGGA CCCTGGGGCC CTCCAGGATC TCGAGGCCTG
    CCAGGCATGA GGGGAGCCAA GGGGCACCGG GGTCTCCAGC TGACTTACAC AGGCTGTGAC
    CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu33005
    Clone ID Related Accession (Same CDS sequence) XM_006538035.4 , XM_006538035.3
    Accession Version XM_006538035.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5541bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product collagen alpha-1(XXVII) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006538035.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGGCACGG GATTCGCGCG GGGGGCCCGA GGCACAGCGG CGTCAGGACC CGGGGGGGGG 
    TTTCTCTTCG CCTGGATCTT GGTCTCATTT ACCTGTCACC TGGCCTCCAC CCAAGGAGCT
    CCTGAAGATG TGGATGTCCT CCAGCGGCTG GGCCTCAGCT GGACGAAGGC CGGGGGTGGC
    CGGAGTCCTA CACCCCCTGG TGTCATTCCT TTCCCATCTG GCTTCATCTT CACACAGCGG
    GCCAAGCTGC AGGCCCCCAC AGCCAATGTT CTTCCCACCA CCCTGGGGCG GGAGCTGGCA
    TTGGTGTTGA GCCTCTGTTC ACATCGTGTG AACCATGCCT TCCTCTTTGC CATCCGCAGC
    AGGAAACACA AGCTGCAACT GGGTCTGCAG TTCCTCCCCG GCAGGACGAT CATCCACCTG
    GGGCCTCGGC AATCAGTGGC CTTCGACTTG GATGTGCATG ATGGGCGCTG GCACCACCTG
    GCCCTAGAGT TGCGTGGCCG CACAGTCACA ATGGTGACAG CCTGTGGGCA GCACAGGGTA
    CCTGTCCCAC TGCCTTCCCG TAGGGACTCC ATGCTTGACC CCCAGGGCTC CTTTCTCTTG
    GGGAAGGTGA ACCCCCGTGC TGTCCAGTTT GAAGGTGCAC TCTGTCAGTT CAGCATCCAC
    CCTGTGGCGC AGGTCGCTCA CAATTACTGT GCCCACCTGA GAGAGCGGTG CCGACAGGTG
    GACACATATA GTCCCCAGGT GGGAACCCTC TTTCCTTGGG ACTCTGGCCC AGCCTTTGCT
    TTGCATCCTG AACCAGCCTT GCTTGGCTTG GGGAATCTGA CCAGAACTCC AGCAACCCTG
    GGGGCCAGGC CTGTAAGCAG GGCACTTGCA GTGACTCTGG CTCCTGCCAT GCCCACCAAG
    CCCCTGAGGA CTGTCCACCC AGATGTCTCT GAACACAGCT CTTCCCAGAC CCCACTGTCA
    CCTGCCAAAC AGTCGGCCAG GAAAACACCT TCCCCCTCCT CGTCAGCTTC CCTGGCTAAT
    TCCACCAGGG TTTATCGTCC AGCTGCTGCC CAGCCAAGAC AGATCACAAC TACTAGCCCC
    ACCAAACGTT CCCCCACCAA GCCTTCTGTT TCTCCCCTTT CAGTTACCCC CATGAAAAGT
    CCCCATGCAA CCCAGAAAAC AGGTGTCCCT TCATTCACAA AGCCTGTTCC ACCCACTCAG
    AAGCCAGCGC CCTTCACCTC CTACCTAGCA CCTTCCAAAG CCTCTAGTCC CACTGTGAGG
    CCTGTTCAGA AGACTTTCAT GACACCCCGA CCTCCAGTCC CCAGCCCCCA GCCCCTGCGC
    CCTACTACTG GCTTATCTAA GAAGTTCACT AACCCCACTG TTGCAAAATC TAAGTCCAAG
    ACGACCAGTT GGGCCAGCAA GCCGGTCTTG GCCCGCTCCA GTGTCCCTAA AACGCTTCAG
    CAAACTGTTT TGTCCCAGTC TCCTGTTTCC TATCTGGGCT CTCAGACACT GGCTCCAGCC
    CTCCCTCCTT TGGGTGTTGG AAATCCTAGA ACGATGCCTC CCACACGTGA CTCTGCTTTA
    ACTCCTGCTG GAAGCAAGAA ATTCACAGGA CGGGAAACCT CCAAGAAAAC CAGACAGAAG
    AGCAGCCCCC GGAAGCCCGA ACCTCTCAGC CCTGGGAAGT CAGCCAGGGA TGCCTCACCA
    AGAGACCTGA CAACCAAGCC TAGCCGGCCG TCCACCCCAG CCCTGGTCCT TGCCCCAGCT
    TACCTCCTGT CCTCCAGCCC CCAGCCCACC AGCAGCAGCT TCCCTTTCTT CCACCTGCTG
    GGCCCTACAC CTTTCCCGAT GCTGATGGGG CCTCCAGGCT CCAAGGGAGA CTGTGGCTTG
    CCGGGTCCCC CTGGGCTTCC AGGATTACCT GGATCTCCGG GCGCACGAGG TCCTCGGGGT
    CCTCCTGGGC CGTACGGGAA CCCAGGCCCA CCTGGCCCTC CAGGAGCCAA AGGACAGAAA
    GGGGACCCAG GACTCTCACC AGGACAGGCT CACGATGGAG CAAAGGGCAA CATGGGCTTG
    CCTGGGCTCT CCGGAAATCC AGGACCCTTG GGAAGGAAGG GACACAAGGG CCATCCTGGA
    GCAGCAGGGC ACCCTGGAGA ACAGGGGCAG CCAGGACCTG AGGGCAGTCC AGGGGCCAAA
    GGTTACCCTG GCAGGCAGGG GTTCCCTGGA CCTGTAGGAG ACCCTGGCCC CAAAGGCAGC
    AGGGGTTACA TTGGTCTTCC GGGGCTCTTT GGCCTGCCAG GGTCGGATGG AGAGCGGGGT
    CTCCCTGGCG TTCCTGGCAA GAGGGGCGAG ATGGGTAGGC CGGGCTTCCC TGGAGACTTT
    GGGGAAAGAG GCCCCCCTGG ACTTGATGGC AACCCTGGAG AAATTGGCCT GCCGGGGCCA
    CCAGGAGTGC TCGGGCTCAT TGGTGACACA GGAGCACTGG GCCCAGTTGG CTACCCAGGA
    CCGAAAGGCA TGAAGGGACT GATGGGCGGC GTGGGGGAGC CCGGACTGAA AGGTGATAAG
    GGTGAACAAG GGGTGCCAGG TGTGTCGGGA GATCCTGGCT TCCAAGGAGA CAAGGGGAGT
    CATGGCTTGC CAGGGCTCCC GGGTGGCCGG GGGAAGCCAG GGCCTCTGGG AAAAGCTGGA
    GACAAAGGAT CACTTGGGTT TCCTGGACCT CCTGGACCTG AGGGTTTCCC AGGAGACATT
    GGCCCTCCAG GGGACAATGG CCCTGAAGGC ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG
    CCTGGACCTC CTGGGCAGCT GGGGCCTGAG GGAGATGAAG GACCCATGGG GCCACCAGGA
    GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT
    GGCTCGAAGG GGGAACCAGG GGATCCTGGA CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT
    ATGGGATTCA TTGGTCTTGT CGGAGAGCCT GGAATTGTTG GGGAGAAGGG AGACCGTGGG
    GTGATGGGAC CCCCAGGTGC ACCTGGACCC AAGGGGTCGA TGGGCCATCC TGGAACACCC
    GGTGGTATTG GGAACCCTGG AGAGCCTGGA CCCTGGGGCC CTCCAGGATC TCGAGGCCTG
    CCAGGCATGA GGGGAGCCAA GGGGCACCGG GGCCCTCGAG GACCTGATGG ACCAGCTGGG
    GAGCAGGGAT CCAAGGGCCT GAAGGGTCGT GTAGGACCTC GGGGCAGACC TGGCCAGCCA
    GGACAGCAGG GTGCAGCTGG TGAGCGAGGC CACTCAGGTG CAAAAGGCTT CCTTGGCATC
    CCTGGTCCCT CAGGCCCTCC AGGTGCCAAG GGCCTCCCAG GAGAACCGGG CTCTCAGGGA
    CCTCAGGGGC CAGTTGGTCC TCCAGGAGAA ATGGGGCCCA AGGGACCGCC GGGTGCAGTG
    GGAGAGCCTG GCCTTCCTGG GGACTCCGGA ATGAAGGGTG ACCTCGGACC TCTGGGCCCC
    CCTGGGGAGC AAGGTCTTAT TGGGCAGCGG GGAGAGCCAG GCCTTGAGGG AGACCATGGC
    CCTGTGGGGC CAGATGGGCT GAAGGGCGAC AGGGGCGACC CAGGGCCCGA TGGCGAGCAT
    GGTGAAAAGG GCCAGGAAGG GCTGAAAGGA GAGGATGGGT CCCCTGGGCC CCCGGGCATC
    ACTGGCGTCC CGGGTCGTGA GGGGAAGCCG GGCAAGCAGG GTGAGAAAGG CCAGAGGGGA
    GCTAAGGGTG CCAAGGGCCA CCAAGGATAT CTGGGAGAGA TGGGCATCCC CGGAGAACCT
    GGGCCCCCTG GGACCCCAGG TCCTAAAGGG TCCCGGGGCA CCCTAGGACC AACGGGTGCT
    CCAGGACGGA TGGGAGCCCA AGGAGAACCA GGATTGGCCG GTTATAATGG ACACAAAGGC
    ATCACAGGAC CCCTCGGGCC TCCCGGGCCC AAAGGCGAGA AGGGGGACCA GGGCGAGGAT
    GGCAAGACCG AGGGGCCCCC TGGGCCACCA GGAGATCGGG GTCCTGTGGG TGATCGAGGA
    GACCGTGGGG AGCCAGGCGA CCCTGGATAC CCTGGTCAAG AGGGAGTTCA AGGCCTCCGT
    GGAGAACCGG GCCAGCAGGG ACAGCCTGGG CATCCAGGAC CCCGGGGGCG CCCAGGACCC
    AAAGGATCAA AAGGCGAAGA GGGCCCAAAG GGAAAGCCAG GCAAGGCTGG GCCATCAGGC
    CGGAGGGGGA CCCAGGGCCT TCAAGGACTG CCAGGCCCCC GAGGCGTGGT GGGGAGACAG
    GGCCCTGAGG GCACTGCTGG ATCTGATGGG ATTCCTGGCA GAGATGGTCG GCCAGGATAT
    CAGGGAGACC AGGGAAATGA TGGGGACCCT GGCCCTGTGG GCCCTGCTGG GAGAAGAGGA
    AATCCAGGTG TGGCTGGCTT GCCTGGAGCA CAGGGGCCTC CGGGATTCAA GGGTGAAAGT
    GGGTTACCTG GGCAACTGGG TCCCCCTGGA AAACGAGGGA CAGAAGGTGG AACAGGGCTT
    CCTGGGAACC AGGGGGAGCC AGGATCCAAA GGCCAGCCGG GTGACTCTGG CGAGATGGGC
    TTCCCAGGAG TGGCTGGCCT CTTTGGACCC AAGGGCCCCC CTGGAGACAT TGGCTTCAAA
    GGCATCCAAG GTCCTCGGGG GCCTCCTGGC TTGATGGGAA AGGAAGGTAT CATTGGCCCC
    CCCGGAATGC TGGGACCTTC TGGACTCCCG GGTCCCAAAG GTGACAGAGG CAGCCGAGGA
    GACTTGGGAC TGCAAGGCCC AAGGGGTCCT CCTGGTCCAA GGGGCCGGCC AGGTCCCCCG
    GGCCCTCCTT GGCATCCCAT CCAGTTTCAG CAAGATGACC TTGGAGCAGC TTTCCAGACA
    TGGATGGATG CTCAAGGAGC CGTCAGATCG GAGCAGGGGT ACAGCTATCC GGACCAGCTG
    GCGCTAGACC AGGGAGGGGA GATCTTCAAA ACCTTACACT ACCTCAGCAA CCTCATCCAG
    AGCATTAAGA CACCCTTGGG CACCAAGGAG AACCCAGCCC GGGTCTGCCG GGACCTCATG
    GACTGTGAGC AGAGGATGGC GGATGGTACC TACTGGGTGG ACCCCAACCT CGGCTGCTCC
    TCTGACACCA TTGAAGTCTC CTGCAACTTT ACACAGGGTG GGCAGACGTG CCTGAAGCCC
    ATCACGGCCT CCAAGGCCGA GTTTGCTGTG AGTCGGGTCC AGATGAATTT CTTGCACCTG
    CTGAGCTCTG AGGGGACACA GCATATCACA ATCCACTGTC TGAACATGAC GGTGTGGCAG
    GAGGGACCGG GACGCTCCTC TGCCAGACAG GCTGTGCGCT TCCGTGCCTG GAACGGACAG
    GTCTTCGAAG CTGGGGGTCA GTTCAGGCCA GAGGTGTCTA TGGATGGCTG CAAGGTCCAT
    GATGGCCGCT GGCATCAGAC ACTGTTCACC TTCCGGACCC AGGACCCCCA GCAGCTGCCC
    ATCGTCAGTG TGGACAATCT CCCGCCTGTC TCATCAGGGA AGCAGTACCG CCTGGAAGTT
    GGACCTGCGT GCTTCCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006538098.1
    CDS504..6044
    Translation

    Target ORF information:

    RefSeq Version XM_006538035.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006538035.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGGCACGG GATTCGCGCG GGGGGCCCGA GGCACAGCGG CGTCAGGACC CGGGGGGGGG 
    TTTCTCTTCG CCTGGATCTT GGTCTCATTT ACCTGTCACC TGGCCTCCAC CCAAGGAGCT
    CCTGAAGATG TGGATGTCCT CCAGCGGCTG GGCCTCAGCT GGACGAAGGC CGGGGGTGGC
    CGGAGTCCTA CACCCCCTGG TGTCATTCCT TTCCCATCTG GCTTCATCTT CACACAGCGG
    GCCAAGCTGC AGGCCCCCAC AGCCAATGTT CTTCCCACCA CCCTGGGGCG GGAGCTGGCA
    TTGGTGTTGA GCCTCTGTTC ACATCGTGTG AACCATGCCT TCCTCTTTGC CATCCGCAGC
    AGGAAACACA AGCTGCAACT GGGTCTGCAG TTCCTCCCCG GCAGGACGAT CATCCACCTG
    GGGCCTCGGC AATCAGTGGC CTTCGACTTG GATGTGCATG ATGGGCGCTG GCACCACCTG
    GCCCTAGAGT TGCGTGGCCG CACAGTCACA ATGGTGACAG CCTGTGGGCA GCACAGGGTA
    CCTGTCCCAC TGCCTTCCCG TAGGGACTCC ATGCTTGACC CCCAGGGCTC CTTTCTCTTG
    GGGAAGGTGA ACCCCCGTGC TGTCCAGTTT GAAGGTGCAC TCTGTCAGTT CAGCATCCAC
    CCTGTGGCGC AGGTCGCTCA CAATTACTGT GCCCACCTGA GAGAGCGGTG CCGACAGGTG
    GACACATATA GTCCCCAGGT GGGAACCCTC TTTCCTTGGG ACTCTGGCCC AGCCTTTGCT
    TTGCATCCTG AACCAGCCTT GCTTGGCTTG GGGAATCTGA CCAGAACTCC AGCAACCCTG
    GGGGCCAGGC CTGTAAGCAG GGCACTTGCA GTGACTCTGG CTCCTGCCAT GCCCACCAAG
    CCCCTGAGGA CTGTCCACCC AGATGTCTCT GAACACAGCT CTTCCCAGAC CCCACTGTCA
    CCTGCCAAAC AGTCGGCCAG GAAAACACCT TCCCCCTCCT CGTCAGCTTC CCTGGCTAAT
    TCCACCAGGG TTTATCGTCC AGCTGCTGCC CAGCCAAGAC AGATCACAAC TACTAGCCCC
    ACCAAACGTT CCCCCACCAA GCCTTCTGTT TCTCCCCTTT CAGTTACCCC CATGAAAAGT
    CCCCATGCAA CCCAGAAAAC AGGTGTCCCT TCATTCACAA AGCCTGTTCC ACCCACTCAG
    AAGCCAGCGC CCTTCACCTC CTACCTAGCA CCTTCCAAAG CCTCTAGTCC CACTGTGAGG
    CCTGTTCAGA AGACTTTCAT GACACCCCGA CCTCCAGTCC CCAGCCCCCA GCCCCTGCGC
    CCTACTACTG GCTTATCTAA GAAGTTCACT AACCCCACTG TTGCAAAATC TAAGTCCAAG
    ACGACCAGTT GGGCCAGCAA GCCGGTCTTG GCCCGCTCCA GTGTCCCTAA AACGCTTCAG
    CAAACTGTTT TGTCCCAGTC TCCTGTTTCC TATCTGGGCT CTCAGACACT GGCTCCAGCC
    CTCCCTCCTT TGGGTGTTGG AAATCCTAGA ACGATGCCTC CCACACGTGA CTCTGCTTTA
    ACTCCTGCTG GAAGCAAGAA ATTCACAGGA CGGGAAACCT CCAAGAAAAC CAGACAGAAG
    AGCAGCCCCC GGAAGCCCGA ACCTCTCAGC CCTGGGAAGT CAGCCAGGGA TGCCTCACCA
    AGAGACCTGA CAACCAAGCC TAGCCGGCCG TCCACCCCAG CCCTGGTCCT TGCCCCAGCT
    TACCTCCTGT CCTCCAGCCC CCAGCCCACC AGCAGCAGCT TCCCTTTCTT CCACCTGCTG
    GGCCCTACAC CTTTCCCGAT GCTGATGGGG CCTCCAGGCT CCAAGGGAGA CTGTGGCTTG
    CCGGGTCCCC CTGGGCTTCC AGGATTACCT GGATCTCCGG GCGCACGAGG TCCTCGGGGT
    CCTCCTGGGC CGTACGGGAA CCCAGGCCCA CCTGGCCCTC CAGGAGCCAA AGGACAGAAA
    GGGGACCCAG GACTCTCACC AGGACAGGCT CACGATGGAG CAAAGGGCAA CATGGGCTTG
    CCTGGGCTCT CCGGAAATCC AGGACCCTTG GGAAGGAAGG GACACAAGGG CCATCCTGGA
    GCAGCAGGGC ACCCTGGAGA ACAGGGGCAG CCAGGACCTG AGGGCAGTCC AGGGGCCAAA
    GGTTACCCTG GCAGGCAGGG GTTCCCTGGA CCTGTAGGAG ACCCTGGCCC CAAAGGCAGC
    AGGGGTTACA TTGGTCTTCC GGGGCTCTTT GGCCTGCCAG GGTCGGATGG AGAGCGGGGT
    CTCCCTGGCG TTCCTGGCAA GAGGGGCGAG ATGGGTAGGC CGGGCTTCCC TGGAGACTTT
    GGGGAAAGAG GCCCCCCTGG ACTTGATGGC AACCCTGGAG AAATTGGCCT GCCGGGGCCA
    CCAGGAGTGC TCGGGCTCAT TGGTGACACA GGAGCACTGG GCCCAGTTGG CTACCCAGGA
    CCGAAAGGCA TGAAGGGACT GATGGGCGGC GTGGGGGAGC CCGGACTGAA AGGTGATAAG
    GGTGAACAAG GGGTGCCAGG TGTGTCGGGA GATCCTGGCT TCCAAGGAGA CAAGGGGAGT
    CATGGCTTGC CAGGGCTCCC GGGTGGCCGG GGGAAGCCAG GGCCTCTGGG AAAAGCTGGA
    GACAAAGGAT CACTTGGGTT TCCTGGACCT CCTGGACCTG AGGGTTTCCC AGGAGACATT
    GGCCCTCCAG GGGACAATGG CCCTGAAGGC ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG
    CCTGGACCTC CTGGGCAGCT GGGGCCTGAG GGAGATGAAG GACCCATGGG GCCACCAGGA
    GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT
    GGCTCGAAGG GGGAACCAGG GGATCCTGGA CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT
    ATGGGATTCA TTGGTCTTGT CGGAGAGCCT GGAATTGTTG GGGAGAAGGG AGACCGTGGG
    GTGATGGGAC CCCCAGGTGC ACCTGGACCC AAGGGGTCGA TGGGCCATCC TGGAACACCC
    GGTGGTATTG GGAACCCTGG AGAGCCTGGA CCCTGGGGCC CTCCAGGATC TCGAGGCCTG
    CCAGGCATGA GGGGAGCCAA GGGGCACCGG GGCCCTCGAG GACCTGATGG ACCAGCTGGG
    GAGCAGGGAT CCAAGGGCCT GAAGGGTCGT GTAGGACCTC GGGGCAGACC TGGCCAGCCA
    GGACAGCAGG GTGCAGCTGG TGAGCGAGGC CACTCAGGTG CAAAAGGCTT CCTTGGCATC
    CCTGGTCCCT CAGGCCCTCC AGGTGCCAAG GGCCTCCCAG GAGAACCGGG CTCTCAGGGA
    CCTCAGGGGC CAGTTGGTCC TCCAGGAGAA ATGGGGCCCA AGGGACCGCC GGGTGCAGTG
    GGAGAGCCTG GCCTTCCTGG GGACTCCGGA ATGAAGGGTG ACCTCGGACC TCTGGGCCCC
    CCTGGGGAGC AAGGTCTTAT TGGGCAGCGG GGAGAGCCAG GCCTTGAGGG AGACCATGGC
    CCTGTGGGGC CAGATGGGCT GAAGGGCGAC AGGGGCGACC CAGGGCCCGA TGGCGAGCAT
    GGTGAAAAGG GCCAGGAAGG GCTGAAAGGA GAGGATGGGT CCCCTGGGCC CCCGGGCATC
    ACTGGCGTCC CGGGTCGTGA GGGGAAGCCG GGCAAGCAGG GTGAGAAAGG CCAGAGGGGA
    GCTAAGGGTG CCAAGGGCCA CCAAGGATAT CTGGGAGAGA TGGGCATCCC CGGAGAACCT
    GGGCCCCCTG GGACCCCAGG TCCTAAAGGG TCCCGGGGCA CCCTAGGACC AACGGGTGCT
    CCAGGACGGA TGGGAGCCCA AGGAGAACCA GGATTGGCCG GTTATAATGG ACACAAAGGC
    ATCACAGGAC CCCTCGGGCC TCCCGGGCCC AAAGGCGAGA AGGGGGACCA GGGCGAGGAT
    GGCAAGACCG AGGGGCCCCC TGGGCCACCA GGAGATCGGG GTCCTGTGGG TGATCGAGGA
    GACCGTGGGG AGCCAGGCGA CCCTGGATAC CCTGGTCAAG AGGGAGTTCA AGGCCTCCGT
    GGAGAACCGG GCCAGCAGGG ACAGCCTGGG CATCCAGGAC CCCGGGGGCG CCCAGGACCC
    AAAGGATCAA AAGGCGAAGA GGGCCCAAAG GGAAAGCCAG GCAAGGCTGG GCCATCAGGC
    CGGAGGGGGA CCCAGGGCCT TCAAGGACTG CCAGGCCCCC GAGGCGTGGT GGGGAGACAG
    GGCCCTGAGG GCACTGCTGG ATCTGATGGG ATTCCTGGCA GAGATGGTCG GCCAGGATAT
    CAGGGAGACC AGGGAAATGA TGGGGACCCT GGCCCTGTGG GCCCTGCTGG GAGAAGAGGA
    AATCCAGGTG TGGCTGGCTT GCCTGGAGCA CAGGGGCCTC CGGGATTCAA GGGTGAAAGT
    GGGTTACCTG GGCAACTGGG TCCCCCTGGA AAACGAGGGA CAGAAGGTGG AACAGGGCTT
    CCTGGGAACC AGGGGGAGCC AGGATCCAAA GGCCAGCCGG GTGACTCTGG CGAGATGGGC
    TTCCCAGGAG TGGCTGGCCT CTTTGGACCC AAGGGCCCCC CTGGAGACAT TGGCTTCAAA
    GGCATCCAAG GTCCTCGGGG GCCTCCTGGC TTGATGGGAA AGGAAGGTAT CATTGGCCCC
    CCCGGAATGC TGGGACCTTC TGGACTCCCG GGTCCCAAAG GTGACAGAGG CAGCCGAGGA
    GACTTGGGAC TGCAAGGCCC AAGGGGTCCT CCTGGTCCAA GGGGCCGGCC AGGTCCCCCG
    GGCCCTCCTT GGCATCCCAT CCAGTTTCAG CAAGATGACC TTGGAGCAGC TTTCCAGACA
    TGGATGGATG CTCAAGGAGC CGTCAGATCG GAGCAGGGGT ACAGCTATCC GGACCAGCTG
    GCGCTAGACC AGGGAGGGGA GATCTTCAAA ACCTTACACT ACCTCAGCAA CCTCATCCAG
    AGCATTAAGA CACCCTTGGG CACCAAGGAG AACCCAGCCC GGGTCTGCCG GGACCTCATG
    GACTGTGAGC AGAGGATGGC GGATGGTACC TACTGGGTGG ACCCCAACCT CGGCTGCTCC
    TCTGACACCA TTGAAGTCTC CTGCAACTTT ACACAGGGTG GGCAGACGTG CCTGAAGCCC
    ATCACGGCCT CCAAGGCCGA GTTTGCTGTG AGTCGGGTCC AGATGAATTT CTTGCACCTG
    CTGAGCTCTG AGGGGACACA GCATATCACA ATCCACTGTC TGAACATGAC GGTGTGGCAG
    GAGGGACCGG GACGCTCCTC TGCCAGACAG GCTGTGCGCT TCCGTGCCTG GAACGGACAG
    GTCTTCGAAG CTGGGGGTCA GTTCAGGCCA GAGGTGTCTA TGGATGGCTG CAAGGTCCAT
    GATGGCCGCT GGCATCAGAC ACTGTTCACC TTCCGGACCC AGGACCCCCA GCAGCTGCCC
    ATCGTCAGTG TGGACAATCT CCCGCCTGTC TCATCAGGGA AGCAGTACCG CCTGGAAGTT
    GGACCTGCGT GCTTCCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu81036
    Clone ID Related Accession (Same CDS sequence) XM_017320282.1
    Accession Version XM_017320282.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5616bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product collagen alpha-1(XXVII) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGGCACGG GATTCGCGCG GGGGGCCCGA GGCACAGCGG CGTCAGGACC CGGGGGGGGG 
    TTTCTCTTCG CCTGGATCTT GGTCTCATTT ACCTGTCACC TGGCCTCCAC CCAAGGAGCT
    CCTGAAGATG TGGATGTCCT CCAGCGGCTG GGCCTCAGCT GGACGAAGGC CGGGGGTGGC
    CGGAGTCCTA CACCCCCTGG TGTCATTCCT TTCCCATCTG GCTTCATCTT CACACAGCGG
    GCCAAGCTGC AGGCCCCCAC AGCCAATGTT CTTCCCACCA CCCTGGGGCG GGAGCTGGCA
    TTGGTGTTGA GCCTCTGTTC ACATCGTGTG AACCATGCCT TCCTCTTTGC CATCCGCAGC
    AGGAAACACA AGCTGCAACT GGGTCTGCAG TTCCTCCCCG GCAGGACGAT CATCCACCTG
    GGGCCTCGGC AATCAGTGGC CTTCGACTTG GATGTGCATG ATGGGCGCTG GCACCACCTG
    GCCCTAGAGT TGCGTGGCCG CACAGTCACA ATGGTGACAG CCTGTGGGCA GCACAGGGTA
    CCTGTCCCAC TGCCTTCCCG TAGGGACTCC ATGCTTGACC CCCAGGGCTC CTTTCTCTTG
    GGGAAGGTGA ACCCCCGTGC TGTCCAGTTT GAAGGTGCAC TCTGTCAGTT CAGCATCCAC
    CCTGTGGCGC AGGTCGCTCA CAATTACTGT GCCCACCTGA GAGAGCGGTG CCGACAGGTG
    GACACATATA GTCCCCAGGT GGGAACCCTC TTTCCTTGGG ACTCTGGCCC AGCCTTTGCT
    TTGCATCCTG AACCAGCCTT GCTTGGCTTG GGGAATCTGA CCAGAACTCC AGCAACCCTG
    GGGGCCAGGC CTGTAAGCAG GGCACTTGCA GTGACTCTGG CTCCTGCCAT GCCCACCAAG
    CCCCTGAGGA CTGTCCACCC AGATGTCTCT GAACACAGCT CTTCCCAGAC CCCACTGTCA
    CCTGCCAAAC AGTCGGCCAG GAAAACACCT TCCCCCTCCT CGTCAGCTTC CCTGGCTAAT
    TCCACCAGGG TTTATCGTCC AGCTGCTGCC CAGCCAAGAC AGATCACAAC TACTAGCCCC
    ACCAAACGTT CCCCCACCAA GCCTTCTGTT TCTCCCCTTT CAGTTACCCC CATGAAAAGT
    CCCCATGCAA CCCAGAAAAC AGGTGTCCCT TCATTCACAA AGCCTGTTCC ACCCACTCAG
    AAGCCAGCGC CCTTCACCTC CTACCTAGCA CCTTCCAAAG CCTCTAGTCC CACTGTGAGG
    CCTGTTCAGA AGACTTTCAT GACACCCCGA CCTCCAGTCC CCAGCCCCCA GCCCCTGCGC
    CCTACTACTG GCTTATCTAA GAAGTTCACT AACCCCACTG TTGCAAAATC TAAGTCCAAG
    ACGACCAGTT GGGCCAGCAA GCCGGTCTTG GCCCGCTCCA GTGTCCCTAA AACGCTTCAG
    CAAACTGTTT TGTCCCAGTC TCCTGTTTCC TATCTGGGCT CTCAGACACT GGCTCCAGCC
    CTCCCTCCTT TGGGTGTTGG AAATCCTAGA ACGATGCCTC CCACACGTGA CTCTGCTTTA
    ACTCCTGCTG GAAGCAAGAA ATTCACAGGA CGGGAAACCT CCAAGAAAAC CAGACAGAAG
    AGCAGCCCCC GGAAGCCCGA ACCTCTCAGC CCTGGGAAGT CAGCCAGGGA TGCCTCACCA
    AGAGACCTGA CAACCAAGCC TAGCCGGCCG TCCACCCCAG CCCTGGTCCT TGCCCCAGCT
    TACCTCCTGT CCTCCAGCCC CCAGCCCACC AGCAGCAGCT TCCCTTTCTT CCACCTGCTG
    GGCCCTACAC CTTTCCCGAT GCTGATGGGG CCTCCAGGCT CCAAGGGAGA CTGTGGCTTG
    CCGGGTCCCC CTGGGCTTCC AGGATTACCT GGATCTCCGG GCGCACGAGG TCCTCGGGGT
    CCTCCTGGGC CGTACGGGAA CCCAGGCCCA CCTGGCCCTC CAGGAGCCAA AGGACAGAAA
    GGGGACCCAG GACTCTCACC AGGACAGGCT CACGATGGAG CAAAGGGCAA CATGGGCTTG
    CCTGGGCTCT CCGGAAATCC AGGACCCTTG GGAAGGAAGG GACACAAGGG CCATCCTGGA
    GCAGCAGGGC ACCCTGGAGA ACAGGGGCAG CCAGGACCTG AGGGCAGTCC AGGGGCCAAA
    GGTTACCCTG GCAGGCAGGG GTTCCCTGGA CCTGTAGGAG ACCCTGGCCC CAAAGGCAGC
    AGGGGTTACA TTGGTCTTCC GGGGCTCTTT GGCCTGCCAG GGTCGGATGG AGAGCGGGGT
    CTCCCTGGCG TTCCTGGCAA GAGGGGCGAG ATGGGTAGGC CGGGCTTCCC TGGAGACTTT
    GGGGAAAGAG GCCCCCCTGG ACTTGATGGC AACCCTGGAG AAATTGGCCT GCCGGGGCCA
    CCAGGAGTGC TCGGGCTCAT TGGTGACACA GGAGCACTGG GCCCAGTTGG CTACCCAGGA
    CCGAAAGGCA TGAAGGGACT GATGGGCGGC GTGGGGGAGC CCGGACTGAA AGGTGATAAG
    GGTGAACAAG GGGTGCCAGG TGTGTCGGGA GATCCTGGCT TCCAAGGAGA CAAGGGGAGT
    CATGGCTTGC CAGGGCTCCC GGGTGGCCGG GGGAAGCCAG GGCCTCTGGG AAAAGCTGGA
    GACAAAGGAT CACTTGGGTT TCCTGGACCT CCTGGACCTG AGGGTTTCCC AGGAGACATT
    GGCCCTCCAG GGGACAATGG CCCTGAAGGC ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG
    CCTGGACCTC CTGGGCAGCT GGGGCCTGAG GGAGATGAAG GACCCATGGG GCCACCAGGA
    GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT
    GGCTCGAAGG GGGAACCAGG GGATCCTGGA CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT
    ATGGGATTCA TTGGTCTTGT CGGAGAGCCT GGAATTGTTG GGGAGAAGGG AGACCGTGGG
    GTGATGGGAC CCCCAGGTGC ACCTGGACCC AAGGGGTCGA TGGGCCATCC TGGAACACCC
    GGTGGTATTG GGAACCCTGG AGAGCCTGGA CCCTGGGGCC CTCCAGGATC TCGAGGCCTG
    CCAGGCATGA GGGGAGCCAA GGGGCACCGG GGCCCTCGAG GACCTGATGG ACCAGCTGGG
    GAGCAGGGAT CCAAGGGCCT GAAGGGTCGT GTAGGACCTC GGGGCAGACC TGGCCAGCCA
    GGACAGCAGG GTGCAGCTGG TGAGCGAGGC CACTCAGGTG CAAAAGGCTT CCTTGGCATC
    CCTGGTCCCT CAGGCCCTCC AGGTGCCAAG GGCCTCCCAG GAGAACCGGG CTCTCAGGGA
    CCTCAGGGGC CAGTTGGTCC TCCAGGAGAA ATGGGGCCCA AGGGACCGCC GGGTGCAGTG
    GGAGAGCCTG GCCTTCCTGG GGACTCCGGA ATGAAGGGTG ACCTCGGACC TCTGGGCCCC
    CCTGGGGAGC AAGGTCTTAT TGGGCAGCGG GGAGAGCCAG GCCTTGAGGG AGACCATGGC
    CCTGTGGGGC CAGATGGGCT GAAGGGCGAC AGGGGCGACC CAGGGCCCGA TGGCGAGCAT
    GGTGAAAAGG GCCAGGAAGG GCTGAAAGGA GAGGATGGGT CCCCTGGGCC CCCGGGCATC
    ACTGGCGTCC CGGGTCGTGA GGGGAAGCCG GGCAAGCAGG GTGAGAAAGG CCAGAGGGGA
    GCTAAGGGTG CCAAGGGCCA CCAAGGATAT CTGGGAGAGA TGGGCATCCC CGGAGAACCT
    GGGCCCCCTG GGACCCCAGG TCCTAAAGGG TCCCGGGGCA CCCTAGGACC AACGGGTGCT
    CCAGGACGGA TGGGAGCCCA AGGAGAACCA GGATTGGCCG GTTATAATGG ACACAAAGGC
    ATCACAGGAC CCCTCGGGCC TCCCGGGCCC AAAGGCGAGA AGGGGGACCA GGGCGAGGAT
    GGCAAGACCG AGGGGCCCCC TGGGCCACCA GGAGATCGGG GTCCTGTGGG TGATCGAGGA
    GACCGTGGGG AGCCAGGCGA CCCTGGATAC CCTGGTCAAG AGGGAGTTCA AGGCCTCCGT
    GGAGAACCGG GCCAGCAGGG ACAGCCTGGG CATCCAGGAC CCCGGGGGCG CCCAGGACCC
    AAAGGATCAA AAGGCGAAGA GGGCCCAAAG GGAAAGCCAG GCAAGGCTGG GCCATCAGGC
    CGGAGGGGGA CCCAGGTGAG TTGCCATAGC TTCTCTGTCA CCTTCTATGT TCTCCTGAGA
    AGGTTTAACC AGCTTCTTCT GTACCTCCAG GGCCTTCAAG GACTGCCAGG CCCCCGAGGC
    GTGGTGGGGA GACAGGGCCC TGAGGGCACT GCTGGATCTG ATGGGATTCC TGGCAGAGAT
    GGTCGGCCAG GATATCAGGG AGACCAGGGA AATGATGGGG ACCCTGGCCC TGTGGGCCCT
    GCTGGGAGAA GAGGAAATCC AGGTGTGGCT GGCTTGCCTG GAGCACAGGG GCCTCCGGGA
    TTCAAGGGTG AAAGTGGGTT ACCTGGGCAA CTGGGTCCCC CTGGAAAACG AGGGACAGAA
    GGTGGAACAG GGCTTCCTGG GAACCAGGGG GAGCCAGGAT CCAAAGGCCA GCCGGGTGAC
    TCTGGCGAGA TGGGCTTCCC AGGAGTGGCT GGCCTCTTTG GACCCAAGGG CCCCCCTGGA
    GACATTGGCT TCAAAGGCAT CCAAGGTCCT CGGGGGCCTC CTGGCTTGAT GGGAAAGGAA
    GGTATCATTG GCCCCCCCGG AATGCTGGGA CCTTCTGGAC TCCCGGGTCC CAAAGGTGAC
    AGAGGCAGCC GAGGAGACTT GGGACTGCAA GGCCCAAGGG GTCCTCCTGG TCCAAGGGGC
    CGGCCAGGTC CCCCGGGCCC TCCTTGGCAT CCCATCCAGT TTCAGCAAGA TGACCTTGGA
    GCAGCTTTCC AGACATGGAT GGATGCTCAA GGAGCCGTCA GATCGGAGCA GGGGTACAGC
    TATCCGGACC AGCTGGCGCT AGACCAGGGA GGGGAGATCT TCAAAACCTT ACACTACCTC
    AGCAACCTCA TCCAGAGCAT TAAGACACCC TTGGGCACCA AGGAGAACCC AGCCCGGGTC
    TGCCGGGACC TCATGGACTG TGAGCAGAGG ATGGCGGATG GTACCTACTG GGTGGACCCC
    AACCTCGGCT GCTCCTCTGA CACCATTGAA GTCTCCTGCA ACTTTACACA GGGTGGGCAG
    ACGTGCCTGA AGCCCATCAC GGCCTCCAAG GCCGAGTTTG CTGTGAGTCG GGTCCAGATG
    AATTTCTTGC ACCTGCTGAG CTCTGAGGGG ACACAGCATA TCACAATCCA CTGTCTGAAC
    ATGACGGTGT GGCAGGAGGG ACCGGGACGC TCCTCTGCCA GACAGGCTGT GCGCTTCCGT
    GCCTGGAACG GACAGGTCTT CGAAGCTGGG GGTCAGTTCA GGCCAGAGGT GTCTATGGAT
    GGCTGCAAGG TCCATGATGG CCGCTGGCAT CAGACACTGT TCACCTTCCG GACCCAGGAC
    CCCCAGCAGC TGCCCATCGT CAGTGTGGAC AATCTCCCGC CTGTCTCATC AGGGAAGCAG
    TACCGCCTGG AAGTTGGACC TGCGTGCTTC CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017175771.1
    CDS507..6122
    Translation

    Target ORF information:

    RefSeq Version XM_017320282.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017320282.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGGCACGG GATTCGCGCG GGGGGCCCGA GGCACAGCGG CGTCAGGACC CGGGGGGGGG 
    TTTCTCTTCG CCTGGATCTT GGTCTCATTT ACCTGTCACC TGGCCTCCAC CCAAGGAGCT
    CCTGAAGATG TGGATGTCCT CCAGCGGCTG GGCCTCAGCT GGACGAAGGC CGGGGGTGGC
    CGGAGTCCTA CACCCCCTGG TGTCATTCCT TTCCCATCTG GCTTCATCTT CACACAGCGG
    GCCAAGCTGC AGGCCCCCAC AGCCAATGTT CTTCCCACCA CCCTGGGGCG GGAGCTGGCA
    TTGGTGTTGA GCCTCTGTTC ACATCGTGTG AACCATGCCT TCCTCTTTGC CATCCGCAGC
    AGGAAACACA AGCTGCAACT GGGTCTGCAG TTCCTCCCCG GCAGGACGAT CATCCACCTG
    GGGCCTCGGC AATCAGTGGC CTTCGACTTG GATGTGCATG ATGGGCGCTG GCACCACCTG
    GCCCTAGAGT TGCGTGGCCG CACAGTCACA ATGGTGACAG CCTGTGGGCA GCACAGGGTA
    CCTGTCCCAC TGCCTTCCCG TAGGGACTCC ATGCTTGACC CCCAGGGCTC CTTTCTCTTG
    GGGAAGGTGA ACCCCCGTGC TGTCCAGTTT GAAGGTGCAC TCTGTCAGTT CAGCATCCAC
    CCTGTGGCGC AGGTCGCTCA CAATTACTGT GCCCACCTGA GAGAGCGGTG CCGACAGGTG
    GACACATATA GTCCCCAGGT GGGAACCCTC TTTCCTTGGG ACTCTGGCCC AGCCTTTGCT
    TTGCATCCTG AACCAGCCTT GCTTGGCTTG GGGAATCTGA CCAGAACTCC AGCAACCCTG
    GGGGCCAGGC CTGTAAGCAG GGCACTTGCA GTGACTCTGG CTCCTGCCAT GCCCACCAAG
    CCCCTGAGGA CTGTCCACCC AGATGTCTCT GAACACAGCT CTTCCCAGAC CCCACTGTCA
    CCTGCCAAAC AGTCGGCCAG GAAAACACCT TCCCCCTCCT CGTCAGCTTC CCTGGCTAAT
    TCCACCAGGG TTTATCGTCC AGCTGCTGCC CAGCCAAGAC AGATCACAAC TACTAGCCCC
    ACCAAACGTT CCCCCACCAA GCCTTCTGTT TCTCCCCTTT CAGTTACCCC CATGAAAAGT
    CCCCATGCAA CCCAGAAAAC AGGTGTCCCT TCATTCACAA AGCCTGTTCC ACCCACTCAG
    AAGCCAGCGC CCTTCACCTC CTACCTAGCA CCTTCCAAAG CCTCTAGTCC CACTGTGAGG
    CCTGTTCAGA AGACTTTCAT GACACCCCGA CCTCCAGTCC CCAGCCCCCA GCCCCTGCGC
    CCTACTACTG GCTTATCTAA GAAGTTCACT AACCCCACTG TTGCAAAATC TAAGTCCAAG
    ACGACCAGTT GGGCCAGCAA GCCGGTCTTG GCCCGCTCCA GTGTCCCTAA AACGCTTCAG
    CAAACTGTTT TGTCCCAGTC TCCTGTTTCC TATCTGGGCT CTCAGACACT GGCTCCAGCC
    CTCCCTCCTT TGGGTGTTGG AAATCCTAGA ACGATGCCTC CCACACGTGA CTCTGCTTTA
    ACTCCTGCTG GAAGCAAGAA ATTCACAGGA CGGGAAACCT CCAAGAAAAC CAGACAGAAG
    AGCAGCCCCC GGAAGCCCGA ACCTCTCAGC CCTGGGAAGT CAGCCAGGGA TGCCTCACCA
    AGAGACCTGA CAACCAAGCC TAGCCGGCCG TCCACCCCAG CCCTGGTCCT TGCCCCAGCT
    TACCTCCTGT CCTCCAGCCC CCAGCCCACC AGCAGCAGCT TCCCTTTCTT CCACCTGCTG
    GGCCCTACAC CTTTCCCGAT GCTGATGGGG CCTCCAGGCT CCAAGGGAGA CTGTGGCTTG
    CCGGGTCCCC CTGGGCTTCC AGGATTACCT GGATCTCCGG GCGCACGAGG TCCTCGGGGT
    CCTCCTGGGC CGTACGGGAA CCCAGGCCCA CCTGGCCCTC CAGGAGCCAA AGGACAGAAA
    GGGGACCCAG GACTCTCACC AGGACAGGCT CACGATGGAG CAAAGGGCAA CATGGGCTTG
    CCTGGGCTCT CCGGAAATCC AGGACCCTTG GGAAGGAAGG GACACAAGGG CCATCCTGGA
    GCAGCAGGGC ACCCTGGAGA ACAGGGGCAG CCAGGACCTG AGGGCAGTCC AGGGGCCAAA
    GGTTACCCTG GCAGGCAGGG GTTCCCTGGA CCTGTAGGAG ACCCTGGCCC CAAAGGCAGC
    AGGGGTTACA TTGGTCTTCC GGGGCTCTTT GGCCTGCCAG GGTCGGATGG AGAGCGGGGT
    CTCCCTGGCG TTCCTGGCAA GAGGGGCGAG ATGGGTAGGC CGGGCTTCCC TGGAGACTTT
    GGGGAAAGAG GCCCCCCTGG ACTTGATGGC AACCCTGGAG AAATTGGCCT GCCGGGGCCA
    CCAGGAGTGC TCGGGCTCAT TGGTGACACA GGAGCACTGG GCCCAGTTGG CTACCCAGGA
    CCGAAAGGCA TGAAGGGACT GATGGGCGGC GTGGGGGAGC CCGGACTGAA AGGTGATAAG
    GGTGAACAAG GGGTGCCAGG TGTGTCGGGA GATCCTGGCT TCCAAGGAGA CAAGGGGAGT
    CATGGCTTGC CAGGGCTCCC GGGTGGCCGG GGGAAGCCAG GGCCTCTGGG AAAAGCTGGA
    GACAAAGGAT CACTTGGGTT TCCTGGACCT CCTGGACCTG AGGGTTTCCC AGGAGACATT
    GGCCCTCCAG GGGACAATGG CCCTGAAGGC ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG
    CCTGGACCTC CTGGGCAGCT GGGGCCTGAG GGAGATGAAG GACCCATGGG GCCACCAGGA
    GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT
    GGCTCGAAGG GGGAACCAGG GGATCCTGGA CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT
    ATGGGATTCA TTGGTCTTGT CGGAGAGCCT GGAATTGTTG GGGAGAAGGG AGACCGTGGG
    GTGATGGGAC CCCCAGGTGC ACCTGGACCC AAGGGGTCGA TGGGCCATCC TGGAACACCC
    GGTGGTATTG GGAACCCTGG AGAGCCTGGA CCCTGGGGCC CTCCAGGATC TCGAGGCCTG
    CCAGGCATGA GGGGAGCCAA GGGGCACCGG GGCCCTCGAG GACCTGATGG ACCAGCTGGG
    GAGCAGGGAT CCAAGGGCCT GAAGGGTCGT GTAGGACCTC GGGGCAGACC TGGCCAGCCA
    GGACAGCAGG GTGCAGCTGG TGAGCGAGGC CACTCAGGTG CAAAAGGCTT CCTTGGCATC
    CCTGGTCCCT CAGGCCCTCC AGGTGCCAAG GGCCTCCCAG GAGAACCGGG CTCTCAGGGA
    CCTCAGGGGC CAGTTGGTCC TCCAGGAGAA ATGGGGCCCA AGGGACCGCC GGGTGCAGTG
    GGAGAGCCTG GCCTTCCTGG GGACTCCGGA ATGAAGGGTG ACCTCGGACC TCTGGGCCCC
    CCTGGGGAGC AAGGTCTTAT TGGGCAGCGG GGAGAGCCAG GCCTTGAGGG AGACCATGGC
    CCTGTGGGGC CAGATGGGCT GAAGGGCGAC AGGGGCGACC CAGGGCCCGA TGGCGAGCAT
    GGTGAAAAGG GCCAGGAAGG GCTGAAAGGA GAGGATGGGT CCCCTGGGCC CCCGGGCATC
    ACTGGCGTCC CGGGTCGTGA GGGGAAGCCG GGCAAGCAGG GTGAGAAAGG CCAGAGGGGA
    GCTAAGGGTG CCAAGGGCCA CCAAGGATAT CTGGGAGAGA TGGGCATCCC CGGAGAACCT
    GGGCCCCCTG GGACCCCAGG TCCTAAAGGG TCCCGGGGCA CCCTAGGACC AACGGGTGCT
    CCAGGACGGA TGGGAGCCCA AGGAGAACCA GGATTGGCCG GTTATAATGG ACACAAAGGC
    ATCACAGGAC CCCTCGGGCC TCCCGGGCCC AAAGGCGAGA AGGGGGACCA GGGCGAGGAT
    GGCAAGACCG AGGGGCCCCC TGGGCCACCA GGAGATCGGG GTCCTGTGGG TGATCGAGGA
    GACCGTGGGG AGCCAGGCGA CCCTGGATAC CCTGGTCAAG AGGGAGTTCA AGGCCTCCGT
    GGAGAACCGG GCCAGCAGGG ACAGCCTGGG CATCCAGGAC CCCGGGGGCG CCCAGGACCC
    AAAGGATCAA AAGGCGAAGA GGGCCCAAAG GGAAAGCCAG GCAAGGCTGG GCCATCAGGC
    CGGAGGGGGA CCCAGGTGAG TTGCCATAGC TTCTCTGTCA CCTTCTATGT TCTCCTGAGA
    AGGTTTAACC AGCTTCTTCT GTACCTCCAG GGCCTTCAAG GACTGCCAGG CCCCCGAGGC
    GTGGTGGGGA GACAGGGCCC TGAGGGCACT GCTGGATCTG ATGGGATTCC TGGCAGAGAT
    GGTCGGCCAG GATATCAGGG AGACCAGGGA AATGATGGGG ACCCTGGCCC TGTGGGCCCT
    GCTGGGAGAA GAGGAAATCC AGGTGTGGCT GGCTTGCCTG GAGCACAGGG GCCTCCGGGA
    TTCAAGGGTG AAAGTGGGTT ACCTGGGCAA CTGGGTCCCC CTGGAAAACG AGGGACAGAA
    GGTGGAACAG GGCTTCCTGG GAACCAGGGG GAGCCAGGAT CCAAAGGCCA GCCGGGTGAC
    TCTGGCGAGA TGGGCTTCCC AGGAGTGGCT GGCCTCTTTG GACCCAAGGG CCCCCCTGGA
    GACATTGGCT TCAAAGGCAT CCAAGGTCCT CGGGGGCCTC CTGGCTTGAT GGGAAAGGAA
    GGTATCATTG GCCCCCCCGG AATGCTGGGA CCTTCTGGAC TCCCGGGTCC CAAAGGTGAC
    AGAGGCAGCC GAGGAGACTT GGGACTGCAA GGCCCAAGGG GTCCTCCTGG TCCAAGGGGC
    CGGCCAGGTC CCCCGGGCCC TCCTTGGCAT CCCATCCAGT TTCAGCAAGA TGACCTTGGA
    GCAGCTTTCC AGACATGGAT GGATGCTCAA GGAGCCGTCA GATCGGAGCA GGGGTACAGC
    TATCCGGACC AGCTGGCGCT AGACCAGGGA GGGGAGATCT TCAAAACCTT ACACTACCTC
    AGCAACCTCA TCCAGAGCAT TAAGACACCC TTGGGCACCA AGGAGAACCC AGCCCGGGTC
    TGCCGGGACC TCATGGACTG TGAGCAGAGG ATGGCGGATG GTACCTACTG GGTGGACCCC
    AACCTCGGCT GCTCCTCTGA CACCATTGAA GTCTCCTGCA ACTTTACACA GGGTGGGCAG
    ACGTGCCTGA AGCCCATCAC GGCCTCCAAG GCCGAGTTTG CTGTGAGTCG GGTCCAGATG
    AATTTCTTGC ACCTGCTGAG CTCTGAGGGG ACACAGCATA TCACAATCCA CTGTCTGAAC
    ATGACGGTGT GGCAGGAGGG ACCGGGACGC TCCTCTGCCA GACAGGCTGT GCGCTTCCGT
    GCCTGGAACG GACAGGTCTT CGAAGCTGGG GGTCAGTTCA GGCCAGAGGT GTCTATGGAT
    GGCTGCAAGG TCCATGATGG CCGCTGGCAT CAGACACTGT TCACCTTCCG GACCCAGGAC
    CCCCAGCAGC TGCCCATCGT CAGTGTGGAC AATCTCCCGC CTGTCTCATC AGGGAAGCAG
    TACCGCCTGG AAGTTGGACC TGCGTGCTTC CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu81037
    Clone ID Related Accession (Same CDS sequence) XM_017320283.1
    Accession Version XM_017320283.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5616bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product collagen alpha-1(XXVII) chain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGCCGTTAG GCACCCGCCT AAGAACCGAC CGGCCAGCGT CACACTTGGG GACCCAGGGG 
    TTTCTCTTCG CCTGGATCTT GGTCTCATTT ACCTGTCACC TGGCCTCCAC CCAAGGAGCT
    CCTGAAGATG TGGATGTCCT CCAGCGGCTG GGCCTCAGCT GGACGAAGGC CGGGGGTGGC
    CGGAGTCCTA CACCCCCTGG TGTCATTCCT TTCCCATCTG GCTTCATCTT CACACAGCGG
    GCCAAGCTGC AGGCCCCCAC AGCCAATGTT CTTCCCACCA CCCTGGGGCG GGAGCTGGCA
    TTGGTGTTGA GCCTCTGTTC ACATCGTGTG AACCATGCCT TCCTCTTTGC CATCCGCAGC
    AGGAAACACA AGCTGCAACT GGGTCTGCAG TTCCTCCCCG GCAGGACGAT CATCCACCTG
    GGGCCTCGGC AATCAGTGGC CTTCGACTTG GATGTGCATG ATGGGCGCTG GCACCACCTG
    GCCCTAGAGT TGCGTGGCCG CACAGTCACA ATGGTGACAG CCTGTGGGCA GCACAGGGTA
    CCTGTCCCAC TGCCTTCCCG TAGGGACTCC ATGCTTGACC CCCAGGGCTC CTTTCTCTTG
    GGGAAGGTGA ACCCCCGTGC TGTCCAGTTT GAAGGTGCAC TCTGTCAGTT CAGCATCCAC
    CCTGTGGCGC AGGTCGCTCA CAATTACTGT GCCCACCTGA GAGAGCGGTG CCGACAGGTG
    GACACATATA GTCCCCAGGT GGGAACCCTC TTTCCTTGGG ACTCTGGCCC AGCCTTTGCT
    TTGCATCCTG AACCAGCCTT GCTTGGCTTG GGGAATCTGA CCAGAACTCC AGCAACCCTG
    GGGGCCAGGC CTGTAAGCAG GGCACTTGCA GTGACTCTGG CTCCTGCCAT GCCCACCAAG
    CCCCTGAGGA CTGTCCACCC AGATGTCTCT GAACACAGCT CTTCCCAGAC CCCACTGTCA
    CCTGCCAAAC AGTCGGCCAG GAAAACACCT TCCCCCTCCT CGTCAGCTTC CCTGGCTAAT
    TCCACCAGGG TTTATCGTCC AGCTGCTGCC CAGCCAAGAC AGATCACAAC TACTAGCCCC
    ACCAAACGTT CCCCCACCAA GCCTTCTGTT TCTCCCCTTT CAGTTACCCC CATGAAAAGT
    CCCCATGCAA CCCAGAAAAC AGGTGTCCCT TCATTCACAA AGCCTGTTCC ACCCACTCAG
    AAGCCAGCGC CCTTCACCTC CTACCTAGCA CCTTCCAAAG CCTCTAGTCC CACTGTGAGG
    CCTGTTCAGA AGACTTTCAT GACACCCCGA CCTCCAGTCC CCAGCCCCCA GCCCCTGCGC
    CCTACTACTG GCTTATCTAA GAAGTTCACT AACCCCACTG TTGCAAAATC TAAGTCCAAG
    ACGACCAGTT GGGCCAGCAA GCCGGTCTTG GCCCGCTCCA GTGTCCCTAA AACGCTTCAG
    CAAACTGTTT TGTCCCAGTC TCCTGTTTCC TATCTGGGCT CTCAGACACT GGCTCCAGCC
    CTCCCTCCTT TGGGTGTTGG AAATCCTAGA ACGATGCCTC CCACACGTGA CTCTGCTTTA
    ACTCCTGCTG GAAGCAAGAA ATTCACAGGA CGGGAAACCT CCAAGAAAAC CAGACAGAAG
    AGCAGCCCCC GGAAGCCCGA ACCTCTCAGC CCTGGGAAGT CAGCCAGGGA TGCCTCACCA
    AGAGACCTGA CAACCAAGCC TAGCCGGCCG TCCACCCCAG CCCTGGTCCT TGCCCCAGCT
    TACCTCCTGT CCTCCAGCCC CCAGCCCACC AGCAGCAGCT TCCCTTTCTT CCACCTGCTG
    GGCCCTACAC CTTTCCCGAT GCTGATGGGG CCTCCAGGCT CCAAGGGAGA CTGTGGCTTG
    CCGGGTCCCC CTGGGCTTCC AGGATTACCT GGATCTCCGG GCGCACGAGG TCCTCGGGGT
    CCTCCTGGGC CGTACGGGAA CCCAGGCCCA CCTGGCCCTC CAGGAGCCAA AGGACAGAAA
    GGGGACCCAG GACTCTCACC AGGACAGGCT CACGATGGAG CAAAGGGCAA CATGGGCTTG
    CCTGGGCTCT CCGGAAATCC AGGACCCTTG GGAAGGAAGG GACACAAGGG CCATCCTGGA
    GCAGCAGGGC ACCCTGGAGA ACAGGGGCAG CCAGGACCTG AGGGCAGTCC AGGGGCCAAA
    GGTTACCCTG GCAGGCAGGG GTTCCCTGGA CCTGTAGGAG ACCCTGGCCC CAAAGGCAGC
    AGGGGTTACA TTGGTCTTCC GGGGCTCTTT GGCCTGCCAG GGTCGGATGG AGAGCGGGGT
    CTCCCTGGCG TTCCTGGCAA GAGGGGCGAG ATGGGTAGGC CGGGCTTCCC TGGAGACTTT
    GGGGAAAGAG GCCCCCCTGG ACTTGATGGC AACCCTGGAG AAATTGGCCT GCCGGGGCCA
    CCAGGAGTGC TCGGGCTCAT TGGTGACACA GGAGCACTGG GCCCAGTTGG CTACCCAGGA
    CCGAAAGGCA TGAAGGGACT GATGGGCGGC GTGGGGGAGC CCGGACTGAA AGGTGATAAG
    GGTGAACAAG GGGTGCCAGG TGTGTCGGGA GATCCTGGCT TCCAAGGAGA CAAGGGGAGT
    CATGGCTTGC CAGGGCTCCC GGGTGGCCGG GGGAAGCCAG GGCCTCTGGG AAAAGCTGGA
    GACAAAGGAT CACTTGGGTT TCCTGGACCT CCTGGACCTG AGGGTTTCCC AGGAGACATT
    GGCCCTCCAG GGGACAATGG CCCTGAAGGC ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG
    CCTGGACCTC CTGGGCAGCT GGGGCCTGAG GGAGATGAAG GACCCATGGG GCCACCAGGA
    GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT
    GGCTCGAAGG GGGAACCAGG GGATCCTGGA CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT
    ATGGGATTCA TTGGTCTTGT CGGAGAGCCT GGAATTGTTG GGGAGAAGGG AGACCGTGGG
    GTGATGGGAC CCCCAGGTGC ACCTGGACCC AAGGGGTCGA TGGGCCATCC TGGAACACCC
    GGTGGTATTG GGAACCCTGG AGAGCCTGGA CCCTGGGGCC CTCCAGGATC TCGAGGCCTG
    CCAGGCATGA GGGGAGCCAA GGGGCACCGG GGCCCTCGAG GACCTGATGG ACCAGCTGGG
    GAGCAGGGAT CCAAGGGCCT GAAGGGTCGT GTAGGACCTC GGGGCAGACC TGGCCAGCCA
    GGACAGCAGG GTGCAGCTGG TGAGCGAGGC CACTCAGGTG CAAAAGGCTT CCTTGGCATC
    CCTGGTCCCT CAGGCCCTCC AGGTGCCAAG GGCCTCCCAG GAGAACCGGG CTCTCAGGGA
    CCTCAGGGGC CAGTTGGTCC TCCAGGAGAA ATGGGGCCCA AGGGACCGCC GGGTGCAGTG
    GGAGAGCCTG GCCTTCCTGG GGACTCCGGA ATGAAGGGTG ACCTCGGACC TCTGGGCCCC
    CCTGGGGAGC AAGGTCTTAT TGGGCAGCGG GGAGAGCCAG GCCTTGAGGG AGACCATGGC
    CCTGTGGGGC CAGATGGGCT GAAGGGCGAC AGGGGCGACC CAGGGCCCGA TGGCGAGCAT
    GGTGAAAAGG GCCAGGAAGG GCTGAAAGGA GAGGATGGGT CCCCTGGGCC CCCGGGCATC
    ACTGGCGTCC CGGGTCGTGA GGGGAAGCCG GGCAAGCAGG GTGAGAAAGG CCAGAGGGGA
    GCTAAGGGTG CCAAGGGCCA CCAAGGATAT CTGGGAGAGA TGGGCATCCC CGGAGAACCT
    GGGCCCCCTG GGACCCCAGG TCCTAAAGGG TCCCGGGGCA CCCTAGGACC AACGGGTGCT
    CCAGGACGGA TGGGAGCCCA AGGAGAACCA GGATTGGCCG GTTATAATGG ACACAAAGGC
    ATCACAGGAC CCCTCGGGCC TCCCGGGCCC AAAGGCGAGA AGGGGGACCA GGGCGAGGAT
    GGCAAGACCG AGGGGCCCCC TGGGCCACCA GGAGATCGGG GTCCTGTGGG TGATCGAGGA
    GACCGTGGGG AGCCAGGCGA CCCTGGATAC CCTGGTCAAG AGGGAGTTCA AGGCCTCCGT
    GGAGAACCGG GCCAGCAGGG ACAGCCTGGG CATCCAGGAC CCCGGGGGCG CCCAGGACCC
    AAAGGATCAA AAGGCGAAGA GGGCCCAAAG GGAAAGCCAG GCAAGGCTGG GCCATCAGGC
    CGGAGGGGGA CCCAGGTGAG TTGCCATAGC TTCTCTGTCA CCTTCTATGT TCTCCTGAGA
    AGGTTTAACC AGCTTCTTCT GTACCTCCAG GGCCTTCAAG GACTGCCAGG CCCCCGAGGC
    GTGGTGGGGA GACAGGGCCC TGAGGGCACT GCTGGATCTG ATGGGATTCC TGGCAGAGAT
    GGTCGGCCAG GATATCAGGG AGACCAGGGA AATGATGGGG ACCCTGGCCC TGTGGGCCCT
    GCTGGGAGAA GAGGAAATCC AGGTGTGGCT GGCTTGCCTG GAGCACAGGG GCCTCCGGGA
    TTCAAGGGTG AAAGTGGGTT ACCTGGGCAA CTGGGTCCCC CTGGAAAACG AGGGACAGAA
    GGTGGAACAG GGCTTCCTGG GAACCAGGGG GAGCCAGGAT CCAAAGGCCA GCCGGGTGAC
    TCTGGCGAGA TGGGCTTCCC AGGAGTGGCT GGCCTCTTTG GACCCAAGGG CCCCCCTGGA
    GACATTGGCT TCAAAGGCAT CCAAGGTCCT CGGGGGCCTC CTGGCTTGAT GGGAAAGGAA
    GGTATCATTG GCCCCCCCGG AATGCTGGGA CCTTCTGGAC TCCCGGGTCC CAAAGGTGAC
    AGAGGCAGCC GAGGAGACTT GGGACTGCAA GGCCCAAGGG GTCCTCCTGG TCCAAGGGGC
    CGGCCAGGTC CCCCGGGCCC TCCTTGGCAT CCCATCCAGT TTCAGCAAGA TGACCTTGGA
    GCAGCTTTCC AGACATGGAT GGATGCTCAA GGAGCCGTCA GATCGGAGCA GGGGTACAGC
    TATCCGGACC AGCTGGCGCT AGACCAGGGA GGGGAGATCT TCAAAACCTT ACACTACCTC
    AGCAACCTCA TCCAGAGCAT TAAGACACCC TTGGGCACCA AGGAGAACCC AGCCCGGGTC
    TGCCGGGACC TCATGGACTG TGAGCAGAGG ATGGCGGATG GTACCTACTG GGTGGACCCC
    AACCTCGGCT GCTCCTCTGA CACCATTGAA GTCTCCTGCA ACTTTACACA GGGTGGGCAG
    ACGTGCCTGA AGCCCATCAC GGCCTCCAAG GCCGAGTTTG CTGTGAGTCG GGTCCAGATG
    AATTTCTTGC ACCTGCTGAG CTCTGAGGGG ACACAGCATA TCACAATCCA CTGTCTGAAC
    ATGACGGTGT GGCAGGAGGG ACCGGGACGC TCCTCTGCCA GACAGGCTGT GCGCTTCCGT
    GCCTGGAACG GACAGGTCTT CGAAGCTGGG GGTCAGTTCA GGCCAGAGGT GTCTATGGAT
    GGCTGCAAGG TCCATGATGG CCGCTGGCAT CAGACACTGT TCACCTTCCG GACCCAGGAC
    CCCCAGCAGC TGCCCATCGT CAGTGTGGAC AATCTCCCGC CTGTCTCATC AGGGAAGCAG
    TACCGCCTGG AAGTTGGACC TGCGTGCTTC CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017175772.1
    CDS341..5956
    Translation

    Target ORF information:

    RefSeq Version XM_017320283.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017320283.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGCCGTTAG GCACCCGCCT AAGAACCGAC CGGCCAGCGT CACACTTGGG GACCCAGGGG 
    TTTCTCTTCG CCTGGATCTT GGTCTCATTT ACCTGTCACC TGGCCTCCAC CCAAGGAGCT
    CCTGAAGATG TGGATGTCCT CCAGCGGCTG GGCCTCAGCT GGACGAAGGC CGGGGGTGGC
    CGGAGTCCTA CACCCCCTGG TGTCATTCCT TTCCCATCTG GCTTCATCTT CACACAGCGG
    GCCAAGCTGC AGGCCCCCAC AGCCAATGTT CTTCCCACCA CCCTGGGGCG GGAGCTGGCA
    TTGGTGTTGA GCCTCTGTTC ACATCGTGTG AACCATGCCT TCCTCTTTGC CATCCGCAGC
    AGGAAACACA AGCTGCAACT GGGTCTGCAG TTCCTCCCCG GCAGGACGAT CATCCACCTG
    GGGCCTCGGC AATCAGTGGC CTTCGACTTG GATGTGCATG ATGGGCGCTG GCACCACCTG
    GCCCTAGAGT TGCGTGGCCG CACAGTCACA ATGGTGACAG CCTGTGGGCA GCACAGGGTA
    CCTGTCCCAC TGCCTTCCCG TAGGGACTCC ATGCTTGACC CCCAGGGCTC CTTTCTCTTG
    GGGAAGGTGA ACCCCCGTGC TGTCCAGTTT GAAGGTGCAC TCTGTCAGTT CAGCATCCAC
    CCTGTGGCGC AGGTCGCTCA CAATTACTGT GCCCACCTGA GAGAGCGGTG CCGACAGGTG
    GACACATATA GTCCCCAGGT GGGAACCCTC TTTCCTTGGG ACTCTGGCCC AGCCTTTGCT
    TTGCATCCTG AACCAGCCTT GCTTGGCTTG GGGAATCTGA CCAGAACTCC AGCAACCCTG
    GGGGCCAGGC CTGTAAGCAG GGCACTTGCA GTGACTCTGG CTCCTGCCAT GCCCACCAAG
    CCCCTGAGGA CTGTCCACCC AGATGTCTCT GAACACAGCT CTTCCCAGAC CCCACTGTCA
    CCTGCCAAAC AGTCGGCCAG GAAAACACCT TCCCCCTCCT CGTCAGCTTC CCTGGCTAAT
    TCCACCAGGG TTTATCGTCC AGCTGCTGCC CAGCCAAGAC AGATCACAAC TACTAGCCCC
    ACCAAACGTT CCCCCACCAA GCCTTCTGTT TCTCCCCTTT CAGTTACCCC CATGAAAAGT
    CCCCATGCAA CCCAGAAAAC AGGTGTCCCT TCATTCACAA AGCCTGTTCC ACCCACTCAG
    AAGCCAGCGC CCTTCACCTC CTACCTAGCA CCTTCCAAAG CCTCTAGTCC CACTGTGAGG
    CCTGTTCAGA AGACTTTCAT GACACCCCGA CCTCCAGTCC CCAGCCCCCA GCCCCTGCGC
    CCTACTACTG GCTTATCTAA GAAGTTCACT AACCCCACTG TTGCAAAATC TAAGTCCAAG
    ACGACCAGTT GGGCCAGCAA GCCGGTCTTG GCCCGCTCCA GTGTCCCTAA AACGCTTCAG
    CAAACTGTTT TGTCCCAGTC TCCTGTTTCC TATCTGGGCT CTCAGACACT GGCTCCAGCC
    CTCCCTCCTT TGGGTGTTGG AAATCCTAGA ACGATGCCTC CCACACGTGA CTCTGCTTTA
    ACTCCTGCTG GAAGCAAGAA ATTCACAGGA CGGGAAACCT CCAAGAAAAC CAGACAGAAG
    AGCAGCCCCC GGAAGCCCGA ACCTCTCAGC CCTGGGAAGT CAGCCAGGGA TGCCTCACCA
    AGAGACCTGA CAACCAAGCC TAGCCGGCCG TCCACCCCAG CCCTGGTCCT TGCCCCAGCT
    TACCTCCTGT CCTCCAGCCC CCAGCCCACC AGCAGCAGCT TCCCTTTCTT CCACCTGCTG
    GGCCCTACAC CTTTCCCGAT GCTGATGGGG CCTCCAGGCT CCAAGGGAGA CTGTGGCTTG
    CCGGGTCCCC CTGGGCTTCC AGGATTACCT GGATCTCCGG GCGCACGAGG TCCTCGGGGT
    CCTCCTGGGC CGTACGGGAA CCCAGGCCCA CCTGGCCCTC CAGGAGCCAA AGGACAGAAA
    GGGGACCCAG GACTCTCACC AGGACAGGCT CACGATGGAG CAAAGGGCAA CATGGGCTTG
    CCTGGGCTCT CCGGAAATCC AGGACCCTTG GGAAGGAAGG GACACAAGGG CCATCCTGGA
    GCAGCAGGGC ACCCTGGAGA ACAGGGGCAG CCAGGACCTG AGGGCAGTCC AGGGGCCAAA
    GGTTACCCTG GCAGGCAGGG GTTCCCTGGA CCTGTAGGAG ACCCTGGCCC CAAAGGCAGC
    AGGGGTTACA TTGGTCTTCC GGGGCTCTTT GGCCTGCCAG GGTCGGATGG AGAGCGGGGT
    CTCCCTGGCG TTCCTGGCAA GAGGGGCGAG ATGGGTAGGC CGGGCTTCCC TGGAGACTTT
    GGGGAAAGAG GCCCCCCTGG ACTTGATGGC AACCCTGGAG AAATTGGCCT GCCGGGGCCA
    CCAGGAGTGC TCGGGCTCAT TGGTGACACA GGAGCACTGG GCCCAGTTGG CTACCCAGGA
    CCGAAAGGCA TGAAGGGACT GATGGGCGGC GTGGGGGAGC CCGGACTGAA AGGTGATAAG
    GGTGAACAAG GGGTGCCAGG TGTGTCGGGA GATCCTGGCT TCCAAGGAGA CAAGGGGAGT
    CATGGCTTGC CAGGGCTCCC GGGTGGCCGG GGGAAGCCAG GGCCTCTGGG AAAAGCTGGA
    GACAAAGGAT CACTTGGGTT TCCTGGACCT CCTGGACCTG AGGGTTTCCC AGGAGACATT
    GGCCCTCCAG GGGACAATGG CCCTGAAGGC ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG
    CCTGGACCTC CTGGGCAGCT GGGGCCTGAG GGAGATGAAG GACCCATGGG GCCACCAGGA
    GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT
    GGCTCGAAGG GGGAACCAGG GGATCCTGGA CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT
    ATGGGATTCA TTGGTCTTGT CGGAGAGCCT GGAATTGTTG GGGAGAAGGG AGACCGTGGG
    GTGATGGGAC CCCCAGGTGC ACCTGGACCC AAGGGGTCGA TGGGCCATCC TGGAACACCC
    GGTGGTATTG GGAACCCTGG AGAGCCTGGA CCCTGGGGCC CTCCAGGATC TCGAGGCCTG
    CCAGGCATGA GGGGAGCCAA GGGGCACCGG GGCCCTCGAG GACCTGATGG ACCAGCTGGG
    GAGCAGGGAT CCAAGGGCCT GAAGGGTCGT GTAGGACCTC GGGGCAGACC TGGCCAGCCA
    GGACAGCAGG GTGCAGCTGG TGAGCGAGGC CACTCAGGTG CAAAAGGCTT CCTTGGCATC
    CCTGGTCCCT CAGGCCCTCC AGGTGCCAAG GGCCTCCCAG GAGAACCGGG CTCTCAGGGA
    CCTCAGGGGC CAGTTGGTCC TCCAGGAGAA ATGGGGCCCA AGGGACCGCC GGGTGCAGTG
    GGAGAGCCTG GCCTTCCTGG GGACTCCGGA ATGAAGGGTG ACCTCGGACC TCTGGGCCCC
    CCTGGGGAGC AAGGTCTTAT TGGGCAGCGG GGAGAGCCAG GCCTTGAGGG AGACCATGGC
    CCTGTGGGGC CAGATGGGCT GAAGGGCGAC AGGGGCGACC CAGGGCCCGA TGGCGAGCAT
    GGTGAAAAGG GCCAGGAAGG GCTGAAAGGA GAGGATGGGT CCCCTGGGCC CCCGGGCATC
    ACTGGCGTCC CGGGTCGTGA GGGGAAGCCG GGCAAGCAGG GTGAGAAAGG CCAGAGGGGA
    GCTAAGGGTG CCAAGGGCCA CCAAGGATAT CTGGGAGAGA TGGGCATCCC CGGAGAACCT
    GGGCCCCCTG GGACCCCAGG TCCTAAAGGG TCCCGGGGCA CCCTAGGACC AACGGGTGCT
    CCAGGACGGA TGGGAGCCCA AGGAGAACCA GGATTGGCCG GTTATAATGG ACACAAAGGC
    ATCACAGGAC CCCTCGGGCC TCCCGGGCCC AAAGGCGAGA AGGGGGACCA GGGCGAGGAT
    GGCAAGACCG AGGGGCCCCC TGGGCCACCA GGAGATCGGG GTCCTGTGGG TGATCGAGGA
    GACCGTGGGG AGCCAGGCGA CCCTGGATAC CCTGGTCAAG AGGGAGTTCA AGGCCTCCGT
    GGAGAACCGG GCCAGCAGGG ACAGCCTGGG CATCCAGGAC CCCGGGGGCG CCCAGGACCC
    AAAGGATCAA AAGGCGAAGA GGGCCCAAAG GGAAAGCCAG GCAAGGCTGG GCCATCAGGC
    CGGAGGGGGA CCCAGGTGAG TTGCCATAGC TTCTCTGTCA CCTTCTATGT TCTCCTGAGA
    AGGTTTAACC AGCTTCTTCT GTACCTCCAG GGCCTTCAAG GACTGCCAGG CCCCCGAGGC
    GTGGTGGGGA GACAGGGCCC TGAGGGCACT GCTGGATCTG ATGGGATTCC TGGCAGAGAT
    GGTCGGCCAG GATATCAGGG AGACCAGGGA AATGATGGGG ACCCTGGCCC TGTGGGCCCT
    GCTGGGAGAA GAGGAAATCC AGGTGTGGCT GGCTTGCCTG GAGCACAGGG GCCTCCGGGA
    TTCAAGGGTG AAAGTGGGTT ACCTGGGCAA CTGGGTCCCC CTGGAAAACG AGGGACAGAA
    GGTGGAACAG GGCTTCCTGG GAACCAGGGG GAGCCAGGAT CCAAAGGCCA GCCGGGTGAC
    TCTGGCGAGA TGGGCTTCCC AGGAGTGGCT GGCCTCTTTG GACCCAAGGG CCCCCCTGGA
    GACATTGGCT TCAAAGGCAT CCAAGGTCCT CGGGGGCCTC CTGGCTTGAT GGGAAAGGAA
    GGTATCATTG GCCCCCCCGG AATGCTGGGA CCTTCTGGAC TCCCGGGTCC CAAAGGTGAC
    AGAGGCAGCC GAGGAGACTT GGGACTGCAA GGCCCAAGGG GTCCTCCTGG TCCAAGGGGC
    CGGCCAGGTC CCCCGGGCCC TCCTTGGCAT CCCATCCAGT TTCAGCAAGA TGACCTTGGA
    GCAGCTTTCC AGACATGGAT GGATGCTCAA GGAGCCGTCA GATCGGAGCA GGGGTACAGC
    TATCCGGACC AGCTGGCGCT AGACCAGGGA GGGGAGATCT TCAAAACCTT ACACTACCTC
    AGCAACCTCA TCCAGAGCAT TAAGACACCC TTGGGCACCA AGGAGAACCC AGCCCGGGTC
    TGCCGGGACC TCATGGACTG TGAGCAGAGG ATGGCGGATG GTACCTACTG GGTGGACCCC
    AACCTCGGCT GCTCCTCTGA CACCATTGAA GTCTCCTGCA ACTTTACACA GGGTGGGCAG
    ACGTGCCTGA AGCCCATCAC GGCCTCCAAG GCCGAGTTTG CTGTGAGTCG GGTCCAGATG
    AATTTCTTGC ACCTGCTGAG CTCTGAGGGG ACACAGCATA TCACAATCCA CTGTCTGAAC
    ATGACGGTGT GGCAGGAGGG ACCGGGACGC TCCTCTGCCA GACAGGCTGT GCGCTTCCGT
    GCCTGGAACG GACAGGTCTT CGAAGCTGGG GGTCAGTTCA GGCCAGAGGT GTCTATGGAT
    GGCTGCAAGG TCCATGATGG CCGCTGGCAT CAGACACTGT TCACCTTCCG GACCCAGGAC
    CCCCAGCAGC TGCCCATCGT CAGTGTGGAC AATCTCCCGC CTGTCTCATC AGGGAAGCAG
    TACCGCCTGG AAGTTGGACC TGCGTGCTTC CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu81038
    Clone ID Related Accession (Same CDS sequence) XM_017320284.1
    Accession Version XM_017320284.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5613bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product collagen alpha-1(XXVII) chain isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGGCACGG GATTCGCGCG GGGGGCCCGA GGCACAGCGG CGTCAGGACC CGGGGGGGGG 
    TTTCTCTTCG CCTGGATCTT GGTCTCATTT ACCTGTCACC TGGCCTCCAC CCAAGGAGCT
    CCTGAAGATG TGGATGTCCT CCAGCGGCTG GGCCTCAGCT GGACGAAGGC CGGGGGTGGC
    CGGAGTCCTA CACCCCCTGG TGTCATTCCT TTCCCATCTG GCTTCATCTT CACACAGCGG
    GCCAAGCTGC AGGCCCCCAC AGCCAATGTT CTTCCCACCA CCCTGGGGCG GGAGCTGGCA
    TTGGTGTTGA GCCTCTGTTC ACATCGTGTG AACCATGCCT TCCTCTTTGC CATCCGCAGC
    AGGAAACACA AGCTGCAACT GGGTCTGCAG TTCCTCCCCG GCAGGACGAT CATCCACCTG
    GGGCCTCGGC AATCAGTGGC CTTCGACTTG GATGTGCATG ATGGGCGCTG GCACCACCTG
    GCCCTAGAGT TGCGTGGCCG CACAGTCACA ATGGTGACAG CCTGTGGGCA GCACAGGGTA
    CCTGTCCCAC TGCCTTCCCG TAGGGACTCC ATGCTTGACC CCCAGGGCTC CTTTCTCTTG
    GGGAAGGTGA ACCCCCGTGC TGTCCAGTTT GAAGGTGCAC TCTGTCAGTT CAGCATCCAC
    CCTGTGGCGC AGGTCGCTCA CAATTACTGT GCCCACCTGA GAGAGCGGTG CCGACAGGTG
    GACACATATA GTCCCCAGGT GGGAACCCTC TTTCCTTGGG ACTCTGGCCC AGCCTTTGCT
    TTGCATCCTG AACCAGCCTT GCTTGGCTTG GGGAATCTGA CCAGAACTCC AGCAACCCTG
    GGGGCCAGGC CTGTAAGCAG GGCACTTGCA GTGACTCTGG CTCCTGCCAT GCCCACCAAG
    CCCCTGAGGA CTGTCCACCC AGATGTCTCT GAACACAGCT CTTCCCAGAC CCCACTGTCA
    CCTGCCAAAC AGTCGGCCAG GAAAACACCT TCCCCCTCCT CGTCAGCTTC CCTGGCTAAT
    TCCACCAGGG TTTATCGTCC AGCTGCTGCC CAGCCAAGAC AGATCACAAC TACTAGCCCC
    ACCAAACGTT CCCCCACCAA GCCTTCTGTT TCTCCCCTTT CAGTTACCCC CATGAAAAGT
    CCCCATGCAA CCCAGAAAAC AGGTGTCCCT TCATTCACAA AGCCTGTTCC ACCCACTCAG
    AAGCCAGCGC CCTTCACCTC CTACCTAGCA CCTTCCAAAG CCTCTAGTCC CACTGTGAGG
    CCTGTTCAGA AGACTTTCAT GACACCCCGA CCTCCAGTCC CCAGCCCCCA GCCCCTGCGC
    CCTACTACTG GCTTATCTAA GAAGTTCACT AACCCCACTG TTGCAAAATC TAAGTCCAAG
    ACGACCAGTT GGGCCAGCAA GCCGGTCTTG GCCCGCTCCA GTGTCCCTAA AACGCTTCAG
    CAAACTGTTT TGTCCCAGTC TCCTGTTTCC TATCTGGGCT CTCAGACACT GGCTCCAGCC
    CTCCCTCCTT TGGGTGTTGG AAATCCTAGA ACGATGCCTC CCACACGTGA CTCTGCTTTA
    ACTCCTGCTG GAAGCAAGAA ATTCACAGGA CGGGAAACCT CCAAGAAAAC CAGACAGAAG
    AGCAGCCCCC GGAAGCCCGA ACCTCTCAGC CCTGGGAAGT CAGCCAGGGA TGCCTCACCA
    AGAGACCTGA CAACCAAGCC TAGCCGGCCG TCCACCCCAG CCCTGGTCCT TGCCCCAGCT
    TACCTCCTGT CCTCCAGCCC CCAGCCCACC AGCAGCAGCT TCCCTTTCTT CCACCTGCTG
    GGCCCTACAC CTTTCCCGAT GCTGATGGGG CCTCCAGGCT CCAAGGGAGA CTGTGGCTTG
    CCGGGTCCCC CTGGGCTTCC AGGATTACCT GGATCTCCGG GCGCACGAGG TCCTCGGGGT
    CCTCCTGGGC CGTACGGGAA CCCAGGCCCA CCTGGCCCTC CAGGAGCCAA AGGACAGAAA
    GGGGACCCAG GACTCTCACC AGGACAGGCT CACGATGGAG CAAAGGGCAA CATGGGCTTG
    CCTGGGCTCT CCGGAAATCC AGGACCCTTG GGAAGGAAGG GACACAAGGG CCATCCTGGA
    GCAGCAGGGC ACCCTGGAGA ACAGGGGCAG CCAGGACCTG AGGGCAGTCC AGGGGCCAAA
    GGTTACCCTG GCAGGCAGGG GTTCCCTGGA CCTGTAGGAG ACCCTGGCCC CAAAGGCAGC
    AGGGGTTACA TTGGTCTTCC GGGGCTCTTT GGCCTGCCAG GGTCGGATGG AGAGCGGGGT
    CTCCCTGGCG TTCCTGGCAA GAGGGGCGAG ATGGGTAGGC CGGGCTTCCC TGGAGACTTT
    GGGGAAAGAG GCCCCCCTGG ACTTGATGGC AACCCTGGAG AAATTGGCCT GCCGGGGCCA
    CCAGGAGTGC TCGGGCTCAT TGGTGACACA GGAGCACTGG GCCCAGTTGG CTACCCAGGA
    CCGAAAGGCA TGAAGGGACT GATGGGCGGC GTGGGGGAGC CCGGACTGAA AGGTGATAAG
    GGTGAACAAG GGGTGCCAGG TGTGTCGGGA GATCCTGGCT TCCAAGGAGA CAAGGGGAGT
    CATGGCTTGC CAGGGCTCCC GGGTGGCCGG GGGAAGCCAG GGCCTCTGGG AAAAGCTGGA
    GACAAAGGAT CACTTGGGTT TCCTGGACCT CCTGGACCTG AGGGTTTCCC AGGAGACATT
    GGCCCTCCAG GGGACAATGG CCCTGAAGGC ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG
    CCTGGACCTC CTGGGCAGCT GGGGCCTGAG GGAGATGAAG GACCCATGGG GCCACCAGGA
    GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT
    GGCTCGAAGG GGGAACCAGG GGATCCTGGA CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT
    ATGGGATTCA TTGGTCTTGT CGGAGAGCCT GGAATTGTTG GGGAGAAGGG AGACCGTGGG
    GTGATGGGAC CCCCAGGTGC ACCTGGACCC AAGGGGTCGA TGGGCCATCC TGGAACACCC
    GGTGGTATTG GGAACCCTGG AGAGCCTGGA CCCTGGGGCC CTCCAGGATC TCGAGGCCTG
    CCAGGCATGA GGGGAGCCAA GGGGCACCGG GGCCCTCGAG GACCTGATGG ACCAGCTGGG
    GAGCAGGGAT CCAAGGGCCT GAAGGGTCGT GTAGGACCTC GGGGCAGACC TGGCCAGCCA
    GGACAGCAGG GTGCAGCTGG TGAGCGAGGC CACTCAGGTG CAAAAGGCTT CCTTGGCATC
    CCTGGTCCCT CAGGCCCTCC AGGTGCCAAG GGCCTCCCAG GAGAACCGGG CTCTCAGGGA
    CCTCAGGGGC CAGTTGGTCC TCCAGGAGAA ATGGGGCCCA AGGGACCGCC GGGTGCAGTG
    GGAGAGCCTG GCCTTCCTGG GGACTCCGGA ATGAAGGGTG ACCTCGGACC TCTGGGCCCC
    CCTGGGGAGC AAGGTCTTAT TGGGCAGCGG GGAGAGCCAG GCCTTGAGGG AGACCATGGC
    CCTGTGGGGC CAGATGGGCT GAAGGGCGAC AGGGGCGACC CAGGGCCCGA TGGCGAGCAT
    GGTGAAAAGG GCCAGGAAGG GCTGAAAGGA GAGGATGGGT CCCCTGGGCC CCCGGGCATC
    ACTGGCGTCC CGGGTCGTGA GGGGAAGCCG GGCAAGCAGG GTGAGAAAGG CCAGAGGGGA
    GCTAAGGGTG CCAAGGGCCA CCAAGGATAT CTGGGAGAGA TGGGCATCCC CGGAGAACCT
    GGGCCCCCTG GGACCCCAGG TCCTAAAGGG TCCCGGGGCA CCCTAGGACC AACGGGTGCT
    CCAGGACGGA TGGGAGCCCA AGGAGAACCA GGATTGGCCG GTTATAATGG ACACAAAGGC
    ATCACAGGAC CCCTCGGGCC TCCCGGGCCC AAAGGCGAGA AGGGGGACCA GGGCGAGGAT
    GGCAAGACCG AGGGGCCCCC TGGGCCACCA GGAGATCGGG GTCCTGTGGG TGATCGAGGA
    GACCGTGGGG AGCCAGGCGA CCCTGGATAC CCTGGTCAAG AGGGAGTTCA AGGCCTCCGT
    GGAGAACCGG GCCAGCAGGG ACAGCCTGGG CATCCAGGAC CCCGGGGGCG CCCAGGACCC
    AAAGGATCAA AAGGCGAAGA GGGCCCAAAG GGAAAGCCAG GCAAGGCTGG GCCATCAGGC
    CGGAGGGGGA CCCAGGTGAG TTGCCATAGC TTCTCTGTCA CCTTCTATGT TCTCCTGAGA
    AGGTTTAACC AGCTTCTTCT GTACCTCCAG GGCCTTCAAG GACTGCCAGG CCCCCGAGGC
    GTGGTGGGGA GACAGGGCCC TGAGGGCACT GCTGGATCTG ATGGGATTCC TGGCAGAGAT
    GGTCGGCCAG GATATCAGGG AGACCAGGGA AATGATGGGG ACCCTGGCCC TGTGGGCCCT
    GCTGGGAGAA GAGGAAATCC AGGTGTGGCT GGCTTGCCTG GAGCACAGGG GCCTCCGGGA
    TTCAAGGGTG AAAGTGGGTT ACCTGGGCAA CTGGGTCCCC CTGGAAAACG AGGGACAGAA
    GGTGGAACAG GGCTTCCTGG GAACCAGGGG GAGCCAGGAT CCAAAGGCCA GCCGGGTGAC
    TCTGGCGAGA TGGGCTTCCC AGGAGTGGCT GGCCTCTTTG GACCCAAGGG CCCCCCTGGA
    GACATTGGCT TCAAAGGCAT CCAAGGTCCT CGGGGGCCTC CTGGCTTGAT GGGAAAGGAA
    GGTATCATTG GCCCCCCCGG AATGCTGGGA CCTTCTGGAC TCCCGGGTCC CAAAGGTGAC
    AGAGGCAGCC GAGGAGACTT GGGACTGCAA GGCCCAAGGG GTCCTCCTGG TCCAAGGGGC
    CGGCCAGGTC CCCCGGGCCC TCCTTGGCAT CCCATCCAGT TTCAGCAAGA TGACCTTGGA
    GCAGCTTTCC AGACATGGAT GGATGCTCAA GGAGCCGTCA GATCGGAGGG GTACAGCTAT
    CCGGACCAGC TGGCGCTAGA CCAGGGAGGG GAGATCTTCA AAACCTTACA CTACCTCAGC
    AACCTCATCC AGAGCATTAA GACACCCTTG GGCACCAAGG AGAACCCAGC CCGGGTCTGC
    CGGGACCTCA TGGACTGTGA GCAGAGGATG GCGGATGGTA CCTACTGGGT GGACCCCAAC
    CTCGGCTGCT CCTCTGACAC CATTGAAGTC TCCTGCAACT TTACACAGGG TGGGCAGACG
    TGCCTGAAGC CCATCACGGC CTCCAAGGCC GAGTTTGCTG TGAGTCGGGT CCAGATGAAT
    TTCTTGCACC TGCTGAGCTC TGAGGGGACA CAGCATATCA CAATCCACTG TCTGAACATG
    ACGGTGTGGC AGGAGGGACC GGGACGCTCC TCTGCCAGAC AGGCTGTGCG CTTCCGTGCC
    TGGAACGGAC AGGTCTTCGA AGCTGGGGGT CAGTTCAGGC CAGAGGTGTC TATGGATGGC
    TGCAAGGTCC ATGATGGCCG CTGGCATCAG ACACTGTTCA CCTTCCGGAC CCAGGACCCC
    CAGCAGCTGC CCATCGTCAG TGTGGACAAT CTCCCGCCTG TCTCATCAGG GAAGCAGTAC
    CGCCTGGAAG TTGGACCTGC GTGCTTCCTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017175773.1
    CDS507..6119
    Translation

    Target ORF information:

    RefSeq Version XM_017320284.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017320284.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGGCACGG GATTCGCGCG GGGGGCCCGA GGCACAGCGG CGTCAGGACC CGGGGGGGGG 
    TTTCTCTTCG CCTGGATCTT GGTCTCATTT ACCTGTCACC TGGCCTCCAC CCAAGGAGCT
    CCTGAAGATG TGGATGTCCT CCAGCGGCTG GGCCTCAGCT GGACGAAGGC CGGGGGTGGC
    CGGAGTCCTA CACCCCCTGG TGTCATTCCT TTCCCATCTG GCTTCATCTT CACACAGCGG
    GCCAAGCTGC AGGCCCCCAC AGCCAATGTT CTTCCCACCA CCCTGGGGCG GGAGCTGGCA
    TTGGTGTTGA GCCTCTGTTC ACATCGTGTG AACCATGCCT TCCTCTTTGC CATCCGCAGC
    AGGAAACACA AGCTGCAACT GGGTCTGCAG TTCCTCCCCG GCAGGACGAT CATCCACCTG
    GGGCCTCGGC AATCAGTGGC CTTCGACTTG GATGTGCATG ATGGGCGCTG GCACCACCTG
    GCCCTAGAGT TGCGTGGCCG CACAGTCACA ATGGTGACAG CCTGTGGGCA GCACAGGGTA
    CCTGTCCCAC TGCCTTCCCG TAGGGACTCC ATGCTTGACC CCCAGGGCTC CTTTCTCTTG
    GGGAAGGTGA ACCCCCGTGC TGTCCAGTTT GAAGGTGCAC TCTGTCAGTT CAGCATCCAC
    CCTGTGGCGC AGGTCGCTCA CAATTACTGT GCCCACCTGA GAGAGCGGTG CCGACAGGTG
    GACACATATA GTCCCCAGGT GGGAACCCTC TTTCCTTGGG ACTCTGGCCC AGCCTTTGCT
    TTGCATCCTG AACCAGCCTT GCTTGGCTTG GGGAATCTGA CCAGAACTCC AGCAACCCTG
    GGGGCCAGGC CTGTAAGCAG GGCACTTGCA GTGACTCTGG CTCCTGCCAT GCCCACCAAG
    CCCCTGAGGA CTGTCCACCC AGATGTCTCT GAACACAGCT CTTCCCAGAC CCCACTGTCA
    CCTGCCAAAC AGTCGGCCAG GAAAACACCT TCCCCCTCCT CGTCAGCTTC CCTGGCTAAT
    TCCACCAGGG TTTATCGTCC AGCTGCTGCC CAGCCAAGAC AGATCACAAC TACTAGCCCC
    ACCAAACGTT CCCCCACCAA GCCTTCTGTT TCTCCCCTTT CAGTTACCCC CATGAAAAGT
    CCCCATGCAA CCCAGAAAAC AGGTGTCCCT TCATTCACAA AGCCTGTTCC ACCCACTCAG
    AAGCCAGCGC CCTTCACCTC CTACCTAGCA CCTTCCAAAG CCTCTAGTCC CACTGTGAGG
    CCTGTTCAGA AGACTTTCAT GACACCCCGA CCTCCAGTCC CCAGCCCCCA GCCCCTGCGC
    CCTACTACTG GCTTATCTAA GAAGTTCACT AACCCCACTG TTGCAAAATC TAAGTCCAAG
    ACGACCAGTT GGGCCAGCAA GCCGGTCTTG GCCCGCTCCA GTGTCCCTAA AACGCTTCAG
    CAAACTGTTT TGTCCCAGTC TCCTGTTTCC TATCTGGGCT CTCAGACACT GGCTCCAGCC
    CTCCCTCCTT TGGGTGTTGG AAATCCTAGA ACGATGCCTC CCACACGTGA CTCTGCTTTA
    ACTCCTGCTG GAAGCAAGAA ATTCACAGGA CGGGAAACCT CCAAGAAAAC CAGACAGAAG
    AGCAGCCCCC GGAAGCCCGA ACCTCTCAGC CCTGGGAAGT CAGCCAGGGA TGCCTCACCA
    AGAGACCTGA CAACCAAGCC TAGCCGGCCG TCCACCCCAG CCCTGGTCCT TGCCCCAGCT
    TACCTCCTGT CCTCCAGCCC CCAGCCCACC AGCAGCAGCT TCCCTTTCTT CCACCTGCTG
    GGCCCTACAC CTTTCCCGAT GCTGATGGGG CCTCCAGGCT CCAAGGGAGA CTGTGGCTTG
    CCGGGTCCCC CTGGGCTTCC AGGATTACCT GGATCTCCGG GCGCACGAGG TCCTCGGGGT
    CCTCCTGGGC CGTACGGGAA CCCAGGCCCA CCTGGCCCTC CAGGAGCCAA AGGACAGAAA
    GGGGACCCAG GACTCTCACC AGGACAGGCT CACGATGGAG CAAAGGGCAA CATGGGCTTG
    CCTGGGCTCT CCGGAAATCC AGGACCCTTG GGAAGGAAGG GACACAAGGG CCATCCTGGA
    GCAGCAGGGC ACCCTGGAGA ACAGGGGCAG CCAGGACCTG AGGGCAGTCC AGGGGCCAAA
    GGTTACCCTG GCAGGCAGGG GTTCCCTGGA CCTGTAGGAG ACCCTGGCCC CAAAGGCAGC
    AGGGGTTACA TTGGTCTTCC GGGGCTCTTT GGCCTGCCAG GGTCGGATGG AGAGCGGGGT
    CTCCCTGGCG TTCCTGGCAA GAGGGGCGAG ATGGGTAGGC CGGGCTTCCC TGGAGACTTT
    GGGGAAAGAG GCCCCCCTGG ACTTGATGGC AACCCTGGAG AAATTGGCCT GCCGGGGCCA
    CCAGGAGTGC TCGGGCTCAT TGGTGACACA GGAGCACTGG GCCCAGTTGG CTACCCAGGA
    CCGAAAGGCA TGAAGGGACT GATGGGCGGC GTGGGGGAGC CCGGACTGAA AGGTGATAAG
    GGTGAACAAG GGGTGCCAGG TGTGTCGGGA GATCCTGGCT TCCAAGGAGA CAAGGGGAGT
    CATGGCTTGC CAGGGCTCCC GGGTGGCCGG GGGAAGCCAG GGCCTCTGGG AAAAGCTGGA
    GACAAAGGAT CACTTGGGTT TCCTGGACCT CCTGGACCTG AGGGTTTCCC AGGAGACATT
    GGCCCTCCAG GGGACAATGG CCCTGAAGGC ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG
    CCTGGACCTC CTGGGCAGCT GGGGCCTGAG GGAGATGAAG GACCCATGGG GCCACCAGGA
    GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT
    GGCTCGAAGG GGGAACCAGG GGATCCTGGA CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT
    ATGGGATTCA TTGGTCTTGT CGGAGAGCCT GGAATTGTTG GGGAGAAGGG AGACCGTGGG
    GTGATGGGAC CCCCAGGTGC ACCTGGACCC AAGGGGTCGA TGGGCCATCC TGGAACACCC
    GGTGGTATTG GGAACCCTGG AGAGCCTGGA CCCTGGGGCC CTCCAGGATC TCGAGGCCTG
    CCAGGCATGA GGGGAGCCAA GGGGCACCGG GGCCCTCGAG GACCTGATGG ACCAGCTGGG
    GAGCAGGGAT CCAAGGGCCT GAAGGGTCGT GTAGGACCTC GGGGCAGACC TGGCCAGCCA
    GGACAGCAGG GTGCAGCTGG TGAGCGAGGC CACTCAGGTG CAAAAGGCTT CCTTGGCATC
    CCTGGTCCCT CAGGCCCTCC AGGTGCCAAG GGCCTCCCAG GAGAACCGGG CTCTCAGGGA
    CCTCAGGGGC CAGTTGGTCC TCCAGGAGAA ATGGGGCCCA AGGGACCGCC GGGTGCAGTG
    GGAGAGCCTG GCCTTCCTGG GGACTCCGGA ATGAAGGGTG ACCTCGGACC TCTGGGCCCC
    CCTGGGGAGC AAGGTCTTAT TGGGCAGCGG GGAGAGCCAG GCCTTGAGGG AGACCATGGC
    CCTGTGGGGC CAGATGGGCT GAAGGGCGAC AGGGGCGACC CAGGGCCCGA TGGCGAGCAT
    GGTGAAAAGG GCCAGGAAGG GCTGAAAGGA GAGGATGGGT CCCCTGGGCC CCCGGGCATC
    ACTGGCGTCC CGGGTCGTGA GGGGAAGCCG GGCAAGCAGG GTGAGAAAGG CCAGAGGGGA
    GCTAAGGGTG CCAAGGGCCA CCAAGGATAT CTGGGAGAGA TGGGCATCCC CGGAGAACCT
    GGGCCCCCTG GGACCCCAGG TCCTAAAGGG TCCCGGGGCA CCCTAGGACC AACGGGTGCT
    CCAGGACGGA TGGGAGCCCA AGGAGAACCA GGATTGGCCG GTTATAATGG ACACAAAGGC
    ATCACAGGAC CCCTCGGGCC TCCCGGGCCC AAAGGCGAGA AGGGGGACCA GGGCGAGGAT
    GGCAAGACCG AGGGGCCCCC TGGGCCACCA GGAGATCGGG GTCCTGTGGG TGATCGAGGA
    GACCGTGGGG AGCCAGGCGA CCCTGGATAC CCTGGTCAAG AGGGAGTTCA AGGCCTCCGT
    GGAGAACCGG GCCAGCAGGG ACAGCCTGGG CATCCAGGAC CCCGGGGGCG CCCAGGACCC
    AAAGGATCAA AAGGCGAAGA GGGCCCAAAG GGAAAGCCAG GCAAGGCTGG GCCATCAGGC
    CGGAGGGGGA CCCAGGTGAG TTGCCATAGC TTCTCTGTCA CCTTCTATGT TCTCCTGAGA
    AGGTTTAACC AGCTTCTTCT GTACCTCCAG GGCCTTCAAG GACTGCCAGG CCCCCGAGGC
    GTGGTGGGGA GACAGGGCCC TGAGGGCACT GCTGGATCTG ATGGGATTCC TGGCAGAGAT
    GGTCGGCCAG GATATCAGGG AGACCAGGGA AATGATGGGG ACCCTGGCCC TGTGGGCCCT
    GCTGGGAGAA GAGGAAATCC AGGTGTGGCT GGCTTGCCTG GAGCACAGGG GCCTCCGGGA
    TTCAAGGGTG AAAGTGGGTT ACCTGGGCAA CTGGGTCCCC CTGGAAAACG AGGGACAGAA
    GGTGGAACAG GGCTTCCTGG GAACCAGGGG GAGCCAGGAT CCAAAGGCCA GCCGGGTGAC
    TCTGGCGAGA TGGGCTTCCC AGGAGTGGCT GGCCTCTTTG GACCCAAGGG CCCCCCTGGA
    GACATTGGCT TCAAAGGCAT CCAAGGTCCT CGGGGGCCTC CTGGCTTGAT GGGAAAGGAA
    GGTATCATTG GCCCCCCCGG AATGCTGGGA CCTTCTGGAC TCCCGGGTCC CAAAGGTGAC
    AGAGGCAGCC GAGGAGACTT GGGACTGCAA GGCCCAAGGG GTCCTCCTGG TCCAAGGGGC
    CGGCCAGGTC CCCCGGGCCC TCCTTGGCAT CCCATCCAGT TTCAGCAAGA TGACCTTGGA
    GCAGCTTTCC AGACATGGAT GGATGCTCAA GGAGCCGTCA GATCGGAGGG GTACAGCTAT
    CCGGACCAGC TGGCGCTAGA CCAGGGAGGG GAGATCTTCA AAACCTTACA CTACCTCAGC
    AACCTCATCC AGAGCATTAA GACACCCTTG GGCACCAAGG AGAACCCAGC CCGGGTCTGC
    CGGGACCTCA TGGACTGTGA GCAGAGGATG GCGGATGGTA CCTACTGGGT GGACCCCAAC
    CTCGGCTGCT CCTCTGACAC CATTGAAGTC TCCTGCAACT TTACACAGGG TGGGCAGACG
    TGCCTGAAGC CCATCACGGC CTCCAAGGCC GAGTTTGCTG TGAGTCGGGT CCAGATGAAT
    TTCTTGCACC TGCTGAGCTC TGAGGGGACA CAGCATATCA CAATCCACTG TCTGAACATG
    ACGGTGTGGC AGGAGGGACC GGGACGCTCC TCTGCCAGAC AGGCTGTGCG CTTCCGTGCC
    TGGAACGGAC AGGTCTTCGA AGCTGGGGGT CAGTTCAGGC CAGAGGTGTC TATGGATGGC
    TGCAAGGTCC ATGATGGCCG CTGGCATCAG ACACTGTTCA CCTTCCGGAC CCAGGACCCC
    CAGCAGCTGC CCATCGTCAG TGTGGACAAT CTCCCGCCTG TCTCATCAGG GAAGCAGTAC
    CGCCTGGAAG TTGGACCTGC GTGCTTCCTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu88564
    Clone ID Related Accession (Same CDS sequence) XM_030253629.1
    Accession Version XM_030253629.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5541bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product collagen alpha-1(XXVII) chain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCCGTTAG GCACCCGCCT AAGAACCGAC CGGCCAGCGT CACACTTGGG GACCCAGGGG 
    TTTCTCTTCG CCTGGATCTT GGTCTCATTT ACCTGTCACC TGGCCTCCAC CCAAGGAGCT
    CCTGAAGATG TGGATGTCCT CCAGCGGCTG GGCCTCAGCT GGACGAAGGC CGGGGGTGGC
    CGGAGTCCTA CACCCCCTGG TGTCATTCCT TTCCCATCTG GCTTCATCTT CACACAGCGG
    GCCAAGCTGC AGGCCCCCAC AGCCAATGTT CTTCCCACCA CCCTGGGGCG GGAGCTGGCA
    TTGGTGTTGA GCCTCTGTTC ACATCGTGTG AACCATGCCT TCCTCTTTGC CATCCGCAGC
    AGGAAACACA AGCTGCAACT GGGTCTGCAG TTCCTCCCCG GCAGGACGAT CATCCACCTG
    GGGCCTCGGC AATCAGTGGC CTTCGACTTG GATGTGCATG ATGGGCGCTG GCACCACCTG
    GCCCTAGAGT TGCGTGGCCG CACAGTCACA ATGGTGACAG CCTGTGGGCA GCACAGGGTA
    CCTGTCCCAC TGCCTTCCCG TAGGGACTCC ATGCTTGACC CCCAGGGCTC CTTTCTCTTG
    GGGAAGGTGA ACCCCCGTGC TGTCCAGTTT GAAGGTGCAC TCTGTCAGTT CAGCATCCAC
    CCTGTGGCGC AGGTCGCTCA CAATTACTGT GCCCACCTGA GAGAGCGGTG CCGACAGGTG
    GACACATATA GTCCCCAGGT GGGAACCCTC TTTCCTTGGG ACTCTGGCCC AGCCTTTGCT
    TTGCATCCTG AACCAGCCTT GCTTGGCTTG GGGAATCTGA CCAGAACTCC AGCAACCCTG
    GGGGCCAGGC CTGTAAGCAG GGCACTTGCA GTGACTCTGG CTCCTGCCAT GCCCACCAAG
    CCCCTGAGGA CTGTCCACCC AGATGTCTCT GAACACAGCT CTTCCCAGAC CCCACTGTCA
    CCTGCCAAAC AGTCGGCCAG GAAAACACCT TCCCCCTCCT CGTCAGCTTC CCTGGCTAAT
    TCCACCAGGG TTTATCGTCC AGCTGCTGCC CAGCCAAGAC AGATCACAAC TACTAGCCCC
    ACCAAACGTT CCCCCACCAA GCCTTCTGTT TCTCCCCTTT CAGTTACCCC CATGAAAAGT
    CCCCATGCAA CCCAGAAAAC AGGTGTCCCT TCATTCACAA AGCCTGTTCC ACCCACTCAG
    AAGCCAGCGC CCTTCACCTC CTACCTAGCA CCTTCCAAAG CCTCTAGTCC CACTGTGAGG
    CCTGTTCAGA AGACTTTCAT GACACCCCGA CCTCCAGTCC CCAGCCCCCA GCCCCTGCGC
    CCTACTACTG GCTTATCTAA GAAGTTCACT AACCCCACTG TTGCAAAATC TAAGTCCAAG
    ACGACCAGTT GGGCCAGCAA GCCGGTCTTG GCCCGCTCCA GTGTCCCTAA AACGCTTCAG
    CAAACTGTTT TGTCCCAGTC TCCTGTTTCC TATCTGGGCT CTCAGACACT GGCTCCAGCC
    CTCCCTCCTT TGGGTGTTGG AAATCCTAGA ACGATGCCTC CCACACGTGA CTCTGCTTTA
    ACTCCTGCTG GAAGCAAGAA ATTCACAGGA CGGGAAACCT CCAAGAAAAC CAGACAGAAG
    AGCAGCCCCC GGAAGCCCGA ACCTCTCAGC CCTGGGAAGT CAGCCAGGGA TGCCTCACCA
    AGAGACCTGA CAACCAAGCC TAGCCGGCCG TCCACCCCAG CCCTGGTCCT TGCCCCAGCT
    TACCTCCTGT CCTCCAGCCC CCAGCCCACC AGCAGCAGCT TCCCTTTCTT CCACCTGCTG
    GGCCCTACAC CTTTCCCGAT GCTGATGGGG CCTCCAGGCT CCAAGGGAGA CTGTGGCTTG
    CCGGGTCCCC CTGGGCTTCC AGGATTACCT GGATCTCCGG GCGCACGAGG TCCTCGGGGT
    CCTCCTGGGC CGTACGGGAA CCCAGGCCCA CCTGGCCCTC CAGGAGCCAA AGGACAGAAA
    GGGGACCCAG GACTCTCACC AGGACAGGCT CACGATGGAG CAAAGGGCAA CATGGGCTTG
    CCTGGGCTCT CCGGAAATCC AGGACCCTTG GGAAGGAAGG GACACAAGGG CCATCCTGGA
    GCAGCAGGGC ACCCTGGAGA ACAGGGGCAG CCAGGACCTG AGGGCAGTCC AGGGGCCAAA
    GGTTACCCTG GCAGGCAGGG GTTCCCTGGA CCTGTAGGAG ACCCTGGCCC CAAAGGCAGC
    AGGGGTTACA TTGGTCTTCC GGGGCTCTTT GGCCTGCCAG GGTCGGATGG AGAGCGGGGT
    CTCCCTGGCG TTCCTGGCAA GAGGGGCGAG ATGGGTAGGC CGGGCTTCCC TGGAGACTTT
    GGGGAAAGAG GCCCCCCTGG ACTTGATGGC AACCCTGGAG AAATTGGCCT GCCGGGGCCA
    CCAGGAGTGC TCGGGCTCAT TGGTGACACA GGAGCACTGG GCCCAGTTGG CTACCCAGGA
    CCGAAAGGCA TGAAGGGACT GATGGGCGGC GTGGGGGAGC CCGGACTGAA AGGTGATAAG
    GGTGAACAAG GGGTGCCAGG TGTGTCGGGA GATCCTGGCT TCCAAGGAGA CAAGGGGAGT
    CATGGCTTGC CAGGGCTCCC GGGTGGCCGG GGGAAGCCAG GGCCTCTGGG AAAAGCTGGA
    GACAAAGGAT CACTTGGGTT TCCTGGACCT CCTGGACCTG AGGGTTTCCC AGGAGACATT
    GGCCCTCCAG GGGACAATGG CCCTGAAGGC ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG
    CCTGGACCTC CTGGGCAGCT GGGGCCTGAG GGAGATGAAG GACCCATGGG GCCACCAGGA
    GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT
    GGCTCGAAGG GGGAACCAGG GGATCCTGGA CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT
    ATGGGATTCA TTGGTCTTGT CGGAGAGCCT GGAATTGTTG GGGAGAAGGG AGACCGTGGG
    GTGATGGGAC CCCCAGGTGC ACCTGGACCC AAGGGGTCGA TGGGCCATCC TGGAACACCC
    GGTGGTATTG GGAACCCTGG AGAGCCTGGA CCCTGGGGCC CTCCAGGATC TCGAGGCCTG
    CCAGGCATGA GGGGAGCCAA GGGGCACCGG GGCCCTCGAG GACCTGATGG ACCAGCTGGG
    GAGCAGGGAT CCAAGGGCCT GAAGGGTCGT GTAGGACCTC GGGGCAGACC TGGCCAGCCA
    GGACAGCAGG GTGCAGCTGG TGAGCGAGGC CACTCAGGTG CAAAAGGCTT CCTTGGCATC
    CCTGGTCCCT CAGGCCCTCC AGGTGCCAAG GGCCTCCCAG GAGAACCGGG CTCTCAGGGA
    CCTCAGGGGC CAGTTGGTCC TCCAGGAGAA ATGGGGCCCA AGGGACCGCC GGGTGCAGTG
    GGAGAGCCTG GCCTTCCTGG GGACTCCGGA ATGAAGGGTG ACCTCGGACC TCTGGGCCCC
    CCTGGGGAGC AAGGTCTTAT TGGGCAGCGG GGAGAGCCAG GCCTTGAGGG AGACCATGGC
    CCTGTGGGGC CAGATGGGCT GAAGGGCGAC AGGGGCGACC CAGGGCCCGA TGGCGAGCAT
    GGTGAAAAGG GCCAGGAAGG GCTGAAAGGA GAGGATGGGT CCCCTGGGCC CCCGGGCATC
    ACTGGCGTCC CGGGTCGTGA GGGGAAGCCG GGCAAGCAGG GTGAGAAAGG CCAGAGGGGA
    GCTAAGGGTG CCAAGGGCCA CCAAGGATAT CTGGGAGAGA TGGGCATCCC CGGAGAACCT
    GGGCCCCCTG GGACCCCAGG TCCTAAAGGG TCCCGGGGCA CCCTAGGACC AACGGGTGCT
    CCAGGACGGA TGGGAGCCCA AGGAGAACCA GGATTGGCCG GTTATAATGG ACACAAAGGC
    ATCACAGGAC CCCTCGGGCC TCCCGGGCCC AAAGGCGAGA AGGGGGACCA GGGCGAGGAT
    GGCAAGACCG AGGGGCCCCC TGGGCCACCA GGAGATCGGG GTCCTGTGGG TGATCGAGGA
    GACCGTGGGG AGCCAGGCGA CCCTGGATAC CCTGGTCAAG AGGGAGTTCA AGGCCTCCGT
    GGAGAACCGG GCCAGCAGGG ACAGCCTGGG CATCCAGGAC CCCGGGGGCG CCCAGGACCC
    AAAGGATCAA AAGGCGAAGA GGGCCCAAAG GGAAAGCCAG GCAAGGCTGG GCCATCAGGC
    CGGAGGGGGA CCCAGGGCCT TCAAGGACTG CCAGGCCCCC GAGGCGTGGT GGGGAGACAG
    GGCCCTGAGG GCACTGCTGG ATCTGATGGG ATTCCTGGCA GAGATGGTCG GCCAGGATAT
    CAGGGAGACC AGGGAAATGA TGGGGACCCT GGCCCTGTGG GCCCTGCTGG GAGAAGAGGA
    AATCCAGGTG TGGCTGGCTT GCCTGGAGCA CAGGGGCCTC CGGGATTCAA GGGTGAAAGT
    GGGTTACCTG GGCAACTGGG TCCCCCTGGA AAACGAGGGA CAGAAGGTGG AACAGGGCTT
    CCTGGGAACC AGGGGGAGCC AGGATCCAAA GGCCAGCCGG GTGACTCTGG CGAGATGGGC
    TTCCCAGGAG TGGCTGGCCT CTTTGGACCC AAGGGCCCCC CTGGAGACAT TGGCTTCAAA
    GGCATCCAAG GTCCTCGGGG GCCTCCTGGC TTGATGGGAA AGGAAGGTAT CATTGGCCCC
    CCCGGAATGC TGGGACCTTC TGGACTCCCG GGTCCCAAAG GTGACAGAGG CAGCCGAGGA
    GACTTGGGAC TGCAAGGCCC AAGGGGTCCT CCTGGTCCAA GGGGCCGGCC AGGTCCCCCG
    GGCCCTCCTT GGCATCCCAT CCAGTTTCAG CAAGATGACC TTGGAGCAGC TTTCCAGACA
    TGGATGGATG CTCAAGGAGC CGTCAGATCG GAGCAGGGGT ACAGCTATCC GGACCAGCTG
    GCGCTAGACC AGGGAGGGGA GATCTTCAAA ACCTTACACT ACCTCAGCAA CCTCATCCAG
    AGCATTAAGA CACCCTTGGG CACCAAGGAG AACCCAGCCC GGGTCTGCCG GGACCTCATG
    GACTGTGAGC AGAGGATGGC GGATGGTACC TACTGGGTGG ACCCCAACCT CGGCTGCTCC
    TCTGACACCA TTGAAGTCTC CTGCAACTTT ACACAGGGTG GGCAGACGTG CCTGAAGCCC
    ATCACGGCCT CCAAGGCCGA GTTTGCTGTG AGTCGGGTCC AGATGAATTT CTTGCACCTG
    CTGAGCTCTG AGGGGACACA GCATATCACA ATCCACTGTC TGAACATGAC GGTGTGGCAG
    GAGGGACCGG GACGCTCCTC TGCCAGACAG GCTGTGCGCT TCCGTGCCTG GAACGGACAG
    GTCTTCGAAG CTGGGGGTCA GTTCAGGCCA GAGGTGTCTA TGGATGGCTG CAAGGTCCAT
    GATGGCCGCT GGCATCAGAC ACTGTTCACC TTCCGGACCC AGGACCCCCA GCAGCTGCCC
    ATCGTCAGTG TGGACAATCT CCCGCCTGTC TCATCAGGGA AGCAGTACCG CCTGGAAGTT
    GGACCTGCGT GCTTCCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030109489.1
    CDS341..5881
    Translation

    Target ORF information:

    RefSeq Version XM_030253629.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030253629.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCCGTTAG GCACCCGCCT AAGAACCGAC CGGCCAGCGT CACACTTGGG GACCCAGGGG 
    TTTCTCTTCG CCTGGATCTT GGTCTCATTT ACCTGTCACC TGGCCTCCAC CCAAGGAGCT
    CCTGAAGATG TGGATGTCCT CCAGCGGCTG GGCCTCAGCT GGACGAAGGC CGGGGGTGGC
    CGGAGTCCTA CACCCCCTGG TGTCATTCCT TTCCCATCTG GCTTCATCTT CACACAGCGG
    GCCAAGCTGC AGGCCCCCAC AGCCAATGTT CTTCCCACCA CCCTGGGGCG GGAGCTGGCA
    TTGGTGTTGA GCCTCTGTTC ACATCGTGTG AACCATGCCT TCCTCTTTGC CATCCGCAGC
    AGGAAACACA AGCTGCAACT GGGTCTGCAG TTCCTCCCCG GCAGGACGAT CATCCACCTG
    GGGCCTCGGC AATCAGTGGC CTTCGACTTG GATGTGCATG ATGGGCGCTG GCACCACCTG
    GCCCTAGAGT TGCGTGGCCG CACAGTCACA ATGGTGACAG CCTGTGGGCA GCACAGGGTA
    CCTGTCCCAC TGCCTTCCCG TAGGGACTCC ATGCTTGACC CCCAGGGCTC CTTTCTCTTG
    GGGAAGGTGA ACCCCCGTGC TGTCCAGTTT GAAGGTGCAC TCTGTCAGTT CAGCATCCAC
    CCTGTGGCGC AGGTCGCTCA CAATTACTGT GCCCACCTGA GAGAGCGGTG CCGACAGGTG
    GACACATATA GTCCCCAGGT GGGAACCCTC TTTCCTTGGG ACTCTGGCCC AGCCTTTGCT
    TTGCATCCTG AACCAGCCTT GCTTGGCTTG GGGAATCTGA CCAGAACTCC AGCAACCCTG
    GGGGCCAGGC CTGTAAGCAG GGCACTTGCA GTGACTCTGG CTCCTGCCAT GCCCACCAAG
    CCCCTGAGGA CTGTCCACCC AGATGTCTCT GAACACAGCT CTTCCCAGAC CCCACTGTCA
    CCTGCCAAAC AGTCGGCCAG GAAAACACCT TCCCCCTCCT CGTCAGCTTC CCTGGCTAAT
    TCCACCAGGG TTTATCGTCC AGCTGCTGCC CAGCCAAGAC AGATCACAAC TACTAGCCCC
    ACCAAACGTT CCCCCACCAA GCCTTCTGTT TCTCCCCTTT CAGTTACCCC CATGAAAAGT
    CCCCATGCAA CCCAGAAAAC AGGTGTCCCT TCATTCACAA AGCCTGTTCC ACCCACTCAG
    AAGCCAGCGC CCTTCACCTC CTACCTAGCA CCTTCCAAAG CCTCTAGTCC CACTGTGAGG
    CCTGTTCAGA AGACTTTCAT GACACCCCGA CCTCCAGTCC CCAGCCCCCA GCCCCTGCGC
    CCTACTACTG GCTTATCTAA GAAGTTCACT AACCCCACTG TTGCAAAATC TAAGTCCAAG
    ACGACCAGTT GGGCCAGCAA GCCGGTCTTG GCCCGCTCCA GTGTCCCTAA AACGCTTCAG
    CAAACTGTTT TGTCCCAGTC TCCTGTTTCC TATCTGGGCT CTCAGACACT GGCTCCAGCC
    CTCCCTCCTT TGGGTGTTGG AAATCCTAGA ACGATGCCTC CCACACGTGA CTCTGCTTTA
    ACTCCTGCTG GAAGCAAGAA ATTCACAGGA CGGGAAACCT CCAAGAAAAC CAGACAGAAG
    AGCAGCCCCC GGAAGCCCGA ACCTCTCAGC CCTGGGAAGT CAGCCAGGGA TGCCTCACCA
    AGAGACCTGA CAACCAAGCC TAGCCGGCCG TCCACCCCAG CCCTGGTCCT TGCCCCAGCT
    TACCTCCTGT CCTCCAGCCC CCAGCCCACC AGCAGCAGCT TCCCTTTCTT CCACCTGCTG
    GGCCCTACAC CTTTCCCGAT GCTGATGGGG CCTCCAGGCT CCAAGGGAGA CTGTGGCTTG
    CCGGGTCCCC CTGGGCTTCC AGGATTACCT GGATCTCCGG GCGCACGAGG TCCTCGGGGT
    CCTCCTGGGC CGTACGGGAA CCCAGGCCCA CCTGGCCCTC CAGGAGCCAA AGGACAGAAA
    GGGGACCCAG GACTCTCACC AGGACAGGCT CACGATGGAG CAAAGGGCAA CATGGGCTTG
    CCTGGGCTCT CCGGAAATCC AGGACCCTTG GGAAGGAAGG GACACAAGGG CCATCCTGGA
    GCAGCAGGGC ACCCTGGAGA ACAGGGGCAG CCAGGACCTG AGGGCAGTCC AGGGGCCAAA
    GGTTACCCTG GCAGGCAGGG GTTCCCTGGA CCTGTAGGAG ACCCTGGCCC CAAAGGCAGC
    AGGGGTTACA TTGGTCTTCC GGGGCTCTTT GGCCTGCCAG GGTCGGATGG AGAGCGGGGT
    CTCCCTGGCG TTCCTGGCAA GAGGGGCGAG ATGGGTAGGC CGGGCTTCCC TGGAGACTTT
    GGGGAAAGAG GCCCCCCTGG ACTTGATGGC AACCCTGGAG AAATTGGCCT GCCGGGGCCA
    CCAGGAGTGC TCGGGCTCAT TGGTGACACA GGAGCACTGG GCCCAGTTGG CTACCCAGGA
    CCGAAAGGCA TGAAGGGACT GATGGGCGGC GTGGGGGAGC CCGGACTGAA AGGTGATAAG
    GGTGAACAAG GGGTGCCAGG TGTGTCGGGA GATCCTGGCT TCCAAGGAGA CAAGGGGAGT
    CATGGCTTGC CAGGGCTCCC GGGTGGCCGG GGGAAGCCAG GGCCTCTGGG AAAAGCTGGA
    GACAAAGGAT CACTTGGGTT TCCTGGACCT CCTGGACCTG AGGGTTTCCC AGGAGACATT
    GGCCCTCCAG GGGACAATGG CCCTGAAGGC ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG
    CCTGGACCTC CTGGGCAGCT GGGGCCTGAG GGAGATGAAG GACCCATGGG GCCACCAGGA
    GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT
    GGCTCGAAGG GGGAACCAGG GGATCCTGGA CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT
    ATGGGATTCA TTGGTCTTGT CGGAGAGCCT GGAATTGTTG GGGAGAAGGG AGACCGTGGG
    GTGATGGGAC CCCCAGGTGC ACCTGGACCC AAGGGGTCGA TGGGCCATCC TGGAACACCC
    GGTGGTATTG GGAACCCTGG AGAGCCTGGA CCCTGGGGCC CTCCAGGATC TCGAGGCCTG
    CCAGGCATGA GGGGAGCCAA GGGGCACCGG GGCCCTCGAG GACCTGATGG ACCAGCTGGG
    GAGCAGGGAT CCAAGGGCCT GAAGGGTCGT GTAGGACCTC GGGGCAGACC TGGCCAGCCA
    GGACAGCAGG GTGCAGCTGG TGAGCGAGGC CACTCAGGTG CAAAAGGCTT CCTTGGCATC
    CCTGGTCCCT CAGGCCCTCC AGGTGCCAAG GGCCTCCCAG GAGAACCGGG CTCTCAGGGA
    CCTCAGGGGC CAGTTGGTCC TCCAGGAGAA ATGGGGCCCA AGGGACCGCC GGGTGCAGTG
    GGAGAGCCTG GCCTTCCTGG GGACTCCGGA ATGAAGGGTG ACCTCGGACC TCTGGGCCCC
    CCTGGGGAGC AAGGTCTTAT TGGGCAGCGG GGAGAGCCAG GCCTTGAGGG AGACCATGGC
    CCTGTGGGGC CAGATGGGCT GAAGGGCGAC AGGGGCGACC CAGGGCCCGA TGGCGAGCAT
    GGTGAAAAGG GCCAGGAAGG GCTGAAAGGA GAGGATGGGT CCCCTGGGCC CCCGGGCATC
    ACTGGCGTCC CGGGTCGTGA GGGGAAGCCG GGCAAGCAGG GTGAGAAAGG CCAGAGGGGA
    GCTAAGGGTG CCAAGGGCCA CCAAGGATAT CTGGGAGAGA TGGGCATCCC CGGAGAACCT
    GGGCCCCCTG GGACCCCAGG TCCTAAAGGG TCCCGGGGCA CCCTAGGACC AACGGGTGCT
    CCAGGACGGA TGGGAGCCCA AGGAGAACCA GGATTGGCCG GTTATAATGG ACACAAAGGC
    ATCACAGGAC CCCTCGGGCC TCCCGGGCCC AAAGGCGAGA AGGGGGACCA GGGCGAGGAT
    GGCAAGACCG AGGGGCCCCC TGGGCCACCA GGAGATCGGG GTCCTGTGGG TGATCGAGGA
    GACCGTGGGG AGCCAGGCGA CCCTGGATAC CCTGGTCAAG AGGGAGTTCA AGGCCTCCGT
    GGAGAACCGG GCCAGCAGGG ACAGCCTGGG CATCCAGGAC CCCGGGGGCG CCCAGGACCC
    AAAGGATCAA AAGGCGAAGA GGGCCCAAAG GGAAAGCCAG GCAAGGCTGG GCCATCAGGC
    CGGAGGGGGA CCCAGGGCCT TCAAGGACTG CCAGGCCCCC GAGGCGTGGT GGGGAGACAG
    GGCCCTGAGG GCACTGCTGG ATCTGATGGG ATTCCTGGCA GAGATGGTCG GCCAGGATAT
    CAGGGAGACC AGGGAAATGA TGGGGACCCT GGCCCTGTGG GCCCTGCTGG GAGAAGAGGA
    AATCCAGGTG TGGCTGGCTT GCCTGGAGCA CAGGGGCCTC CGGGATTCAA GGGTGAAAGT
    GGGTTACCTG GGCAACTGGG TCCCCCTGGA AAACGAGGGA CAGAAGGTGG AACAGGGCTT
    CCTGGGAACC AGGGGGAGCC AGGATCCAAA GGCCAGCCGG GTGACTCTGG CGAGATGGGC
    TTCCCAGGAG TGGCTGGCCT CTTTGGACCC AAGGGCCCCC CTGGAGACAT TGGCTTCAAA
    GGCATCCAAG GTCCTCGGGG GCCTCCTGGC TTGATGGGAA AGGAAGGTAT CATTGGCCCC
    CCCGGAATGC TGGGACCTTC TGGACTCCCG GGTCCCAAAG GTGACAGAGG CAGCCGAGGA
    GACTTGGGAC TGCAAGGCCC AAGGGGTCCT CCTGGTCCAA GGGGCCGGCC AGGTCCCCCG
    GGCCCTCCTT GGCATCCCAT CCAGTTTCAG CAAGATGACC TTGGAGCAGC TTTCCAGACA
    TGGATGGATG CTCAAGGAGC CGTCAGATCG GAGCAGGGGT ACAGCTATCC GGACCAGCTG
    GCGCTAGACC AGGGAGGGGA GATCTTCAAA ACCTTACACT ACCTCAGCAA CCTCATCCAG
    AGCATTAAGA CACCCTTGGG CACCAAGGAG AACCCAGCCC GGGTCTGCCG GGACCTCATG
    GACTGTGAGC AGAGGATGGC GGATGGTACC TACTGGGTGG ACCCCAACCT CGGCTGCTCC
    TCTGACACCA TTGAAGTCTC CTGCAACTTT ACACAGGGTG GGCAGACGTG CCTGAAGCCC
    ATCACGGCCT CCAAGGCCGA GTTTGCTGTG AGTCGGGTCC AGATGAATTT CTTGCACCTG
    CTGAGCTCTG AGGGGACACA GCATATCACA ATCCACTGTC TGAACATGAC GGTGTGGCAG
    GAGGGACCGG GACGCTCCTC TGCCAGACAG GCTGTGCGCT TCCGTGCCTG GAACGGACAG
    GTCTTCGAAG CTGGGGGTCA GTTCAGGCCA GAGGTGTCTA TGGATGGCTG CAAGGTCCAT
    GATGGCCGCT GGCATCAGAC ACTGTTCACC TTCCGGACCC AGGACCCCCA GCAGCTGCCC
    ATCGTCAGTG TGGACAATCT CCCGCCTGTC TCATCAGGGA AGCAGTACCG CCTGGAAGTT
    GGACCTGCGT GCTTCCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu88565
    Clone ID Related Accession (Same CDS sequence) XM_030253630.1
    Accession Version XM_030253630.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5031bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product collagen alpha-1(XXVII) chain isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGTGACAG CCTGTGGGCA GCACAGGGTA CCTGTCCCAC TGCCTTCCCG TAGGGACTCC 
    ATGCTTGACC CCCAGGGCTC CTTTCTCTTG GGGAAGGTGA ACCCCCGTGC TGTCCAGTTT
    GAAGGTGCAC TCTGTCAGTT CAGCATCCAC CCTGTGGCGC AGGTCGCTCA CAATTACTGT
    GCCCACCTGA GAGAGCGGTG CCGACAGGTG GACACATATA GTCCCCAGGT GGGAACCCTC
    TTTCCTTGGG ACTCTGGCCC AGCCTTTGCT TTGCATCCTG AACCAGCCTT GCTTGGCTTG
    GGGAATCTGA CCAGAACTCC AGCAACCCTG GGGGCCAGGC CTGTAAGCAG GGCACTTGCA
    GTGACTCTGG CTCCTGCCAT GCCCACCAAG CCCCTGAGGA CTGTCCACCC AGATGTCTCT
    GAACACAGCT CTTCCCAGAC CCCACTGTCA CCTGCCAAAC AGTCGGCCAG GAAAACACCT
    TCCCCCTCCT CGTCAGCTTC CCTGGCTAAT TCCACCAGGG TTTATCGTCC AGCTGCTGCC
    CAGCCAAGAC AGATCACAAC TACTAGCCCC ACCAAACGTT CCCCCACCAA GCCTTCTGTT
    TCTCCCCTTT CAGTTACCCC CATGAAAAGT CCCCATGCAA CCCAGAAAAC AGGTGTCCCT
    TCATTCACAA AGCCTGTTCC ACCCACTCAG AAGCCAGCGC CCTTCACCTC CTACCTAGCA
    CCTTCCAAAG CCTCTAGTCC CACTGTGAGG CCTGTTCAGA AGACTTTCAT GACACCCCGA
    CCTCCAGTCC CCAGCCCCCA GCCCCTGCGC CCTACTACTG GCTTATCTAA GAAGTTCACT
    AACCCCACTG TTGCAAAATC TAAGTCCAAG ACGACCAGTT GGGCCAGCAA GCCGGTCTTG
    GCCCGCTCCA GTGTCCCTAA AACGCTTCAG CAAACTGTTT TGTCCCAGTC TCCTGTTTCC
    TATCTGGGCT CTCAGACACT GGCTCCAGCC CTCCCTCCTT TGGGTGTTGG AAATCCTAGA
    ACGATGCCTC CCACACGTGA CTCTGCTTTA ACTCCTGCTG GAAGCAAGAA ATTCACAGGA
    CGGGAAACCT CCAAGAAAAC CAGACAGAAG AGCAGCCCCC GGAAGCCCGA ACCTCTCAGC
    CCTGGGAAGT CAGCCAGGGA TGCCTCACCA AGAGACCTGA CAACCAAGCC TAGCCGGCCG
    TCCACCCCAG CCCTGGTCCT TGCCCCAGCT TACCTCCTGT CCTCCAGCCC CCAGCCCACC
    AGCAGCAGCT TCCCTTTCTT CCACCTGCTG GGCCCTACAC CTTTCCCGAT GCTGATGGGG
    CCTCCAGGCT CCAAGGGAGA CTGTGGCTTG CCGGGTCCCC CTGGGCTTCC AGGATTACCT
    GGATCTCCGG GCGCACGAGG TCCTCGGGGT CCTCCTGGGC CGTACGGGAA CCCAGGCCCA
    CCTGGCCCTC CAGGAGCCAA AGGACAGAAA GGGGACCCAG GACTCTCACC AGGACAGGCT
    CACGATGGAG CAAAGGGCAA CATGGGCTTG CCTGGGCTCT CCGGAAATCC AGGACCCTTG
    GGAAGGAAGG GACACAAGGG CCATCCTGGA GCAGCAGGGC ACCCTGGAGA ACAGGGGCAG
    CCAGGACCTG AGGGCAGTCC AGGGGCCAAA GGTTACCCTG GCAGGCAGGG GTTCCCTGGA
    CCTGTAGGAG ACCCTGGCCC CAAAGGCAGC AGGGGTTACA TTGGTCTTCC GGGGCTCTTT
    GGCCTGCCAG GGTCGGATGG AGAGCGGGGT CTCCCTGGCG TTCCTGGCAA GAGGGGCGAG
    ATGGGTAGGC CGGGCTTCCC TGGAGACTTT GGGGAAAGAG GCCCCCCTGG ACTTGATGGC
    AACCCTGGAG AAATTGGCCT GCCGGGGCCA CCAGGAGTGC TCGGGCTCAT TGGTGACACA
    GGAGCACTGG GCCCAGTTGG CTACCCAGGA CCGAAAGGCA TGAAGGGACT GATGGGCGGC
    GTGGGGGAGC CCGGACTGAA AGGTGATAAG GGTGAACAAG GGGTGCCAGG TGTGTCGGGA
    GATCCTGGCT TCCAAGGAGA CAAGGGGAGT CATGGCTTGC CAGGGCTCCC GGGTGGCCGG
    GGGAAGCCAG GGCCTCTGGG AAAAGCTGGA GACAAAGGAT CACTTGGGTT TCCTGGACCT
    CCTGGACCTG AGGGTTTCCC AGGAGACATT GGCCCTCCAG GGGACAATGG CCCTGAAGGC
    ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG CCTGGACCTC CTGGGCAGCT GGGGCCTGAG
    GGAGATGAAG GACCCATGGG GCCACCAGGA GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG
    AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT GGCTCGAAGG GGGAACCAGG GGATCCTGGA
    CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT ATGGGATTCA TTGGTCTTGT CGGAGAGCCT
    GGAATTGTTG GGGAGAAGGG AGACCGTGGG GTGATGGGAC CCCCAGGTGC ACCTGGACCC
    AAGGGGTCGA TGGGCCATCC TGGAACACCC GGTGGTATTG GGAACCCTGG AGAGCCTGGA
    CCCTGGGGCC CTCCAGGATC TCGAGGCCTG CCAGGCATGA GGGGAGCCAA GGGGCACCGG
    GGCCCTCGAG GACCTGATGG ACCAGCTGGG GAGCAGGGAT CCAAGGGCCT GAAGGGTCGT
    GTAGGACCTC GGGGCAGACC TGGCCAGCCA GGACAGCAGG GTGCAGCTGG TGAGCGAGGC
    CACTCAGGTG CAAAAGGCTT CCTTGGCATC CCTGGTCCCT CAGGCCCTCC AGGTGCCAAG
    GGCCTCCCAG GAGAACCGGG CTCTCAGGGA CCTCAGGGGC CAGTTGGTCC TCCAGGAGAA
    ATGGGGCCCA AGGGACCGCC GGGTGCAGTG GGAGAGCCTG GCCTTCCTGG GGACTCCGGA
    ATGAAGGGTG ACCTCGGACC TCTGGGCCCC CCTGGGGAGC AAGGTCTTAT TGGGCAGCGG
    GGAGAGCCAG GCCTTGAGGG AGACCATGGC CCTGTGGGGC CAGATGGGCT GAAGGGCGAC
    AGGGGCGACC CAGGGCCCGA TGGCGAGCAT GGTGAAAAGG GCCAGGAAGG GCTGAAAGGA
    GAGGATGGGT CCCCTGGGCC CCCGGGCATC ACTGGCGTCC CGGGTCGTGA GGGGAAGCCG
    GGCAAGCAGG GTGAGAAAGG CCAGAGGGGA GCTAAGGGTG CCAAGGGCCA CCAAGGATAT
    CTGGGAGAGA TGGGCATCCC CGGAGAACCT GGGCCCCCTG GGACCCCAGG TCCTAAAGGG
    TCCCGGGGCA CCCTAGGACC AACGGGTGCT CCAGGACGGA TGGGAGCCCA AGGAGAACCA
    GGATTGGCCG GTTATAATGG ACACAAAGGC ATCACAGGAC CCCTCGGGCC TCCCGGGCCC
    AAAGGCGAGA AGGGGGACCA GGGCGAGGAT GGCAAGACCG AGGGGCCCCC TGGGCCACCA
    GGAGATCGGG GTCCTGTGGG TGATCGAGGA GACCGTGGGG AGCCAGGCGA CCCTGGATAC
    CCTGGTCAAG AGGGAGTTCA AGGCCTCCGT GGAGAACCGG GCCAGCAGGG ACAGCCTGGG
    CATCCAGGAC CCCGGGGGCG CCCAGGACCC AAAGGATCAA AAGGCGAAGA GGGCCCAAAG
    GGAAAGCCAG GCAAGGCTGG GCCATCAGGC CGGAGGGGGA CCCAGGGCCT TCAAGGACTG
    CCAGGCCCCC GAGGCGTGGT GGGGAGACAG GGCCCTGAGG GCACTGCTGG ATCTGATGGG
    ATTCCTGGCA GAGATGGTCG GCCAGGATAT CAGGGAGACC AGGGAAATGA TGGGGACCCT
    GGCCCTGTGG GCCCTGCTGG GAGAAGAGGA AATCCAGGTG TGGCTGGCTT GCCTGGAGCA
    CAGGGGCCTC CGGGATTCAA GGGTGAAAGT GGGTTACCTG GGCAACTGGG TCCCCCTGGA
    AAACGAGGGA CAGAAGGTGG AACAGGGCTT CCTGGGAACC AGGGGGAGCC AGGATCCAAA
    GGCCAGCCGG GTGACTCTGG CGAGATGGGC TTCCCAGGAG TGGCTGGCCT CTTTGGACCC
    AAGGGCCCCC CTGGAGACAT TGGCTTCAAA GGCATCCAAG GTCCTCGGGG GCCTCCTGGC
    TTGATGGGAA AGGAAGGTAT CATTGGCCCC CCCGGAATGC TGGGACCTTC TGGACTCCCG
    GGTCCCAAAG GTGACAGAGG CAGCCGAGGA GACTTGGGAC TGCAAGGCCC AAGGGGTCCT
    CCTGGTCCAA GGGGCCGGCC AGGTCCCCCG GGCCCTCCTT GGCATCCCAT CCAGTTTCAG
    CAAGATGACC TTGGAGCAGC TTTCCAGACA TGGATGGATG CTCAAGGAGC CGTCAGATCG
    GAGCAGGGGT ACAGCTATCC GGACCAGCTG GCGCTAGACC AGGGAGGGGA GATCTTCAAA
    ACCTTACACT ACCTCAGCAA CCTCATCCAG AGCATTAAGA CACCCTTGGG CACCAAGGAG
    AACCCAGCCC GGGTCTGCCG GGACCTCATG GACTGTGAGC AGAGGATGGC GGATGGTACC
    TACTGGGTGG ACCCCAACCT CGGCTGCTCC TCTGACACCA TTGAAGTCTC CTGCAACTTT
    ACACAGGGTG GGCAGACGTG CCTGAAGCCC ATCACGGCCT CCAAGGCCGA GTTTGCTGTG
    AGTCGGGTCC AGATGAATTT CTTGCACCTG CTGAGCTCTG AGGGGACACA GCATATCACA
    ATCCACTGTC TGAACATGAC GGTGTGGCAG GAGGGACCGG GACGCTCCTC TGCCAGACAG
    GCTGTGCGCT TCCGTGCCTG GAACGGACAG GTCTTCGAAG CTGGGGGTCA GTTCAGGCCA
    GAGGTGTCTA TGGATGGCTG CAAGGTCCAT GATGGCCGCT GGCATCAGAC ACTGTTCACC
    TTCCGGACCC AGGACCCCCA GCAGCTGCCC ATCGTCAGTG TGGACAATCT CCCGCCTGTC
    TCATCAGGGA AGCAGTACCG CCTGGAAGTT GGACCTGCGT GCTTCCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030109490.1
    CDS551..5581
    Translation

    Target ORF information:

    RefSeq Version XM_030253630.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030253630.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGTGACAG CCTGTGGGCA GCACAGGGTA CCTGTCCCAC TGCCTTCCCG TAGGGACTCC 
    ATGCTTGACC CCCAGGGCTC CTTTCTCTTG GGGAAGGTGA ACCCCCGTGC TGTCCAGTTT
    GAAGGTGCAC TCTGTCAGTT CAGCATCCAC CCTGTGGCGC AGGTCGCTCA CAATTACTGT
    GCCCACCTGA GAGAGCGGTG CCGACAGGTG GACACATATA GTCCCCAGGT GGGAACCCTC
    TTTCCTTGGG ACTCTGGCCC AGCCTTTGCT TTGCATCCTG AACCAGCCTT GCTTGGCTTG
    GGGAATCTGA CCAGAACTCC AGCAACCCTG GGGGCCAGGC CTGTAAGCAG GGCACTTGCA
    GTGACTCTGG CTCCTGCCAT GCCCACCAAG CCCCTGAGGA CTGTCCACCC AGATGTCTCT
    GAACACAGCT CTTCCCAGAC CCCACTGTCA CCTGCCAAAC AGTCGGCCAG GAAAACACCT
    TCCCCCTCCT CGTCAGCTTC CCTGGCTAAT TCCACCAGGG TTTATCGTCC AGCTGCTGCC
    CAGCCAAGAC AGATCACAAC TACTAGCCCC ACCAAACGTT CCCCCACCAA GCCTTCTGTT
    TCTCCCCTTT CAGTTACCCC CATGAAAAGT CCCCATGCAA CCCAGAAAAC AGGTGTCCCT
    TCATTCACAA AGCCTGTTCC ACCCACTCAG AAGCCAGCGC CCTTCACCTC CTACCTAGCA
    CCTTCCAAAG CCTCTAGTCC CACTGTGAGG CCTGTTCAGA AGACTTTCAT GACACCCCGA
    CCTCCAGTCC CCAGCCCCCA GCCCCTGCGC CCTACTACTG GCTTATCTAA GAAGTTCACT
    AACCCCACTG TTGCAAAATC TAAGTCCAAG ACGACCAGTT GGGCCAGCAA GCCGGTCTTG
    GCCCGCTCCA GTGTCCCTAA AACGCTTCAG CAAACTGTTT TGTCCCAGTC TCCTGTTTCC
    TATCTGGGCT CTCAGACACT GGCTCCAGCC CTCCCTCCTT TGGGTGTTGG AAATCCTAGA
    ACGATGCCTC CCACACGTGA CTCTGCTTTA ACTCCTGCTG GAAGCAAGAA ATTCACAGGA
    CGGGAAACCT CCAAGAAAAC CAGACAGAAG AGCAGCCCCC GGAAGCCCGA ACCTCTCAGC
    CCTGGGAAGT CAGCCAGGGA TGCCTCACCA AGAGACCTGA CAACCAAGCC TAGCCGGCCG
    TCCACCCCAG CCCTGGTCCT TGCCCCAGCT TACCTCCTGT CCTCCAGCCC CCAGCCCACC
    AGCAGCAGCT TCCCTTTCTT CCACCTGCTG GGCCCTACAC CTTTCCCGAT GCTGATGGGG
    CCTCCAGGCT CCAAGGGAGA CTGTGGCTTG CCGGGTCCCC CTGGGCTTCC AGGATTACCT
    GGATCTCCGG GCGCACGAGG TCCTCGGGGT CCTCCTGGGC CGTACGGGAA CCCAGGCCCA
    CCTGGCCCTC CAGGAGCCAA AGGACAGAAA GGGGACCCAG GACTCTCACC AGGACAGGCT
    CACGATGGAG CAAAGGGCAA CATGGGCTTG CCTGGGCTCT CCGGAAATCC AGGACCCTTG
    GGAAGGAAGG GACACAAGGG CCATCCTGGA GCAGCAGGGC ACCCTGGAGA ACAGGGGCAG
    CCAGGACCTG AGGGCAGTCC AGGGGCCAAA GGTTACCCTG GCAGGCAGGG GTTCCCTGGA
    CCTGTAGGAG ACCCTGGCCC CAAAGGCAGC AGGGGTTACA TTGGTCTTCC GGGGCTCTTT
    GGCCTGCCAG GGTCGGATGG AGAGCGGGGT CTCCCTGGCG TTCCTGGCAA GAGGGGCGAG
    ATGGGTAGGC CGGGCTTCCC TGGAGACTTT GGGGAAAGAG GCCCCCCTGG ACTTGATGGC
    AACCCTGGAG AAATTGGCCT GCCGGGGCCA CCAGGAGTGC TCGGGCTCAT TGGTGACACA
    GGAGCACTGG GCCCAGTTGG CTACCCAGGA CCGAAAGGCA TGAAGGGACT GATGGGCGGC
    GTGGGGGAGC CCGGACTGAA AGGTGATAAG GGTGAACAAG GGGTGCCAGG TGTGTCGGGA
    GATCCTGGCT TCCAAGGAGA CAAGGGGAGT CATGGCTTGC CAGGGCTCCC GGGTGGCCGG
    GGGAAGCCAG GGCCTCTGGG AAAAGCTGGA GACAAAGGAT CACTTGGGTT TCCTGGACCT
    CCTGGACCTG AGGGTTTCCC AGGAGACATT GGCCCTCCAG GGGACAATGG CCCTGAAGGC
    ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG CCTGGACCTC CTGGGCAGCT GGGGCCTGAG
    GGAGATGAAG GACCCATGGG GCCACCAGGA GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG
    AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT GGCTCGAAGG GGGAACCAGG GGATCCTGGA
    CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT ATGGGATTCA TTGGTCTTGT CGGAGAGCCT
    GGAATTGTTG GGGAGAAGGG AGACCGTGGG GTGATGGGAC CCCCAGGTGC ACCTGGACCC
    AAGGGGTCGA TGGGCCATCC TGGAACACCC GGTGGTATTG GGAACCCTGG AGAGCCTGGA
    CCCTGGGGCC CTCCAGGATC TCGAGGCCTG CCAGGCATGA GGGGAGCCAA GGGGCACCGG
    GGCCCTCGAG GACCTGATGG ACCAGCTGGG GAGCAGGGAT CCAAGGGCCT GAAGGGTCGT
    GTAGGACCTC GGGGCAGACC TGGCCAGCCA GGACAGCAGG GTGCAGCTGG TGAGCGAGGC
    CACTCAGGTG CAAAAGGCTT CCTTGGCATC CCTGGTCCCT CAGGCCCTCC AGGTGCCAAG
    GGCCTCCCAG GAGAACCGGG CTCTCAGGGA CCTCAGGGGC CAGTTGGTCC TCCAGGAGAA
    ATGGGGCCCA AGGGACCGCC GGGTGCAGTG GGAGAGCCTG GCCTTCCTGG GGACTCCGGA
    ATGAAGGGTG ACCTCGGACC TCTGGGCCCC CCTGGGGAGC AAGGTCTTAT TGGGCAGCGG
    GGAGAGCCAG GCCTTGAGGG AGACCATGGC CCTGTGGGGC CAGATGGGCT GAAGGGCGAC
    AGGGGCGACC CAGGGCCCGA TGGCGAGCAT GGTGAAAAGG GCCAGGAAGG GCTGAAAGGA
    GAGGATGGGT CCCCTGGGCC CCCGGGCATC ACTGGCGTCC CGGGTCGTGA GGGGAAGCCG
    GGCAAGCAGG GTGAGAAAGG CCAGAGGGGA GCTAAGGGTG CCAAGGGCCA CCAAGGATAT
    CTGGGAGAGA TGGGCATCCC CGGAGAACCT GGGCCCCCTG GGACCCCAGG TCCTAAAGGG
    TCCCGGGGCA CCCTAGGACC AACGGGTGCT CCAGGACGGA TGGGAGCCCA AGGAGAACCA
    GGATTGGCCG GTTATAATGG ACACAAAGGC ATCACAGGAC CCCTCGGGCC TCCCGGGCCC
    AAAGGCGAGA AGGGGGACCA GGGCGAGGAT GGCAAGACCG AGGGGCCCCC TGGGCCACCA
    GGAGATCGGG GTCCTGTGGG TGATCGAGGA GACCGTGGGG AGCCAGGCGA CCCTGGATAC
    CCTGGTCAAG AGGGAGTTCA AGGCCTCCGT GGAGAACCGG GCCAGCAGGG ACAGCCTGGG
    CATCCAGGAC CCCGGGGGCG CCCAGGACCC AAAGGATCAA AAGGCGAAGA GGGCCCAAAG
    GGAAAGCCAG GCAAGGCTGG GCCATCAGGC CGGAGGGGGA CCCAGGGCCT TCAAGGACTG
    CCAGGCCCCC GAGGCGTGGT GGGGAGACAG GGCCCTGAGG GCACTGCTGG ATCTGATGGG
    ATTCCTGGCA GAGATGGTCG GCCAGGATAT CAGGGAGACC AGGGAAATGA TGGGGACCCT
    GGCCCTGTGG GCCCTGCTGG GAGAAGAGGA AATCCAGGTG TGGCTGGCTT GCCTGGAGCA
    CAGGGGCCTC CGGGATTCAA GGGTGAAAGT GGGTTACCTG GGCAACTGGG TCCCCCTGGA
    AAACGAGGGA CAGAAGGTGG AACAGGGCTT CCTGGGAACC AGGGGGAGCC AGGATCCAAA
    GGCCAGCCGG GTGACTCTGG CGAGATGGGC TTCCCAGGAG TGGCTGGCCT CTTTGGACCC
    AAGGGCCCCC CTGGAGACAT TGGCTTCAAA GGCATCCAAG GTCCTCGGGG GCCTCCTGGC
    TTGATGGGAA AGGAAGGTAT CATTGGCCCC CCCGGAATGC TGGGACCTTC TGGACTCCCG
    GGTCCCAAAG GTGACAGAGG CAGCCGAGGA GACTTGGGAC TGCAAGGCCC AAGGGGTCCT
    CCTGGTCCAA GGGGCCGGCC AGGTCCCCCG GGCCCTCCTT GGCATCCCAT CCAGTTTCAG
    CAAGATGACC TTGGAGCAGC TTTCCAGACA TGGATGGATG CTCAAGGAGC CGTCAGATCG
    GAGCAGGGGT ACAGCTATCC GGACCAGCTG GCGCTAGACC AGGGAGGGGA GATCTTCAAA
    ACCTTACACT ACCTCAGCAA CCTCATCCAG AGCATTAAGA CACCCTTGGG CACCAAGGAG
    AACCCAGCCC GGGTCTGCCG GGACCTCATG GACTGTGAGC AGAGGATGGC GGATGGTACC
    TACTGGGTGG ACCCCAACCT CGGCTGCTCC TCTGACACCA TTGAAGTCTC CTGCAACTTT
    ACACAGGGTG GGCAGACGTG CCTGAAGCCC ATCACGGCCT CCAAGGCCGA GTTTGCTGTG
    AGTCGGGTCC AGATGAATTT CTTGCACCTG CTGAGCTCTG AGGGGACACA GCATATCACA
    ATCCACTGTC TGAACATGAC GGTGTGGCAG GAGGGACCGG GACGCTCCTC TGCCAGACAG
    GCTGTGCGCT TCCGTGCCTG GAACGGACAG GTCTTCGAAG CTGGGGGTCA GTTCAGGCCA
    GAGGTGTCTA TGGATGGCTG CAAGGTCCAT GATGGCCGCT GGCATCAGAC ACTGTTCACC
    TTCCGGACCC AGGACCCCCA GCAGCTGCCC ATCGTCAGTG TGGACAATCT CCCGCCTGTC
    TCATCAGGGA AGCAGTACCG CCTGGAAGTT GGACCTGCGT GCTTCCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu88566
    Clone ID Related Accession (Same CDS sequence) XM_030253631.1
    Accession Version XM_030253631.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4677bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product collagen alpha-1(XXVII) chain isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGGCACGG GATTCGCGCG GGGGGCCCGA GGCACAGCGG CGTCAGGACC CGGGGGGGGG 
    TTTCTCTTCG CCTGGATCTT GGTCTCATTT ACCTGTCACC TGGCCTCCAC CCAAGGAGCT
    CCTGAAGATG TGGATGTCCT CCAGCGGCTG GGCCTCAGCT GGACGAAGGC CGGGGGTGGC
    CGGAGTCCTA CACCCCCTGG TGTCATTCCT TTCCCATCTG GCTTCATCTT CACACAGCGG
    GCCAAGCTGC AGGCCCCCAC AGCCAATGTT CTTCCCACCA CCCTGGGGCG GGAGCTGGCA
    TTGGTGTTGA GCCTCTGTTC ACATCGTGTG AACCATGCCT TCCTCTTTGC CATCCGCAGC
    AGGAAACACA AGCTGCAACT GGGTCTGCAG TTCCTCCCCG GCAGGACGAT CATCCACCTG
    GGGCCTCGGC AATCAGTGGC CTTCGACTTG GATGTGCATG ATGGGCGCTG GCACCACCTG
    GCCCTAGAGT TGCGTGGCCG CACAGTCACA ATGGTGACAG CCTGTGGGCA GCACAGGGTA
    CCTGTCCCAC TGCCTTCCCG TAGGGACTCC ATGCTTGACC CCCAGGGCTC CTTTCTCTTG
    GGGAAGGTGA ACCCCCGTGC TGTCCAGTTT GAAGGTGCAC TCTGTCAGTT CAGCATCCAC
    CCTGTGGCGC AGGTCGCTCA CAATTACTGT GCCCACCTGA GAGAGCGGTG CCGACAGGTG
    GACACATATA GTCCCCAGGT GGGAACCCTC TTTCCTTGGG ACTCTGGCCC AGCCTTTGCT
    TTGCATCCTG AACCAGCCTT GCTTGGCTTG GGGAATCTGA CCAGAACTCC AGCAACCCTG
    GGGGCCAGGC CTGTAAGCAG GGCACTTGCA GTGACTCTGG CTCCTGCCAT GCCCACCAAG
    CCCCTGAGGA CTGTCCACCC AGATGTCTCT GAACACAGCT CTTCCCAGAC CCCACTGTCA
    CCTGCCAAAC AGTCGGCCAG GAAAACACCT TCCCCCTCCT CGTCAGCTTC CCTGGCTAAT
    TCCACCAGGG TTTATCGTCC AGCTGCTGCC CAGCCAAGAC AGATCACAAC TACTAGCCCC
    ACCAAACGTT CCCCCACCAA GCCTTCTGTT TCTCCCCTTT CAGTTACCCC CATGAAAAGT
    CCCCATGCAA CCCAGAAAAC AGGTGTCCCT TCATTCACAA AGCCTGTTCC ACCCACTCAG
    AAGCCAGCGC CCTTCACCTC CTACCTAGCA CCTTCCAAAG CCTCTAGTCC CACTGTGAGG
    CCTGTTCAGA AGACTTTCAT GACACCCCGA CCTCCAGTCC CCAGCCCCCA GCCCCTGCGC
    CCTACTACTG GCTTATCTAA GAAGTTCACT AACCCCACTG TTGCAAAATC TAAGTCCAAG
    ACGACCAGTT GGGCCAGCAA GCCGGTCTTG GCCCGCTCCA GTGTCCCTAA AACGCTTCAG
    CAAACTGTTT TGTCCCAGTC TCCTGTTTCC TATCTGGGCT CTCAGACACT GGCTCCAGCC
    CTCCCTCCTT TGGGTGTTGG AAATCCTAGA ACGATGCCTC CCACACGTGA CTCTGCTTTA
    ACTCCTGCTG GAAGCAAGAA ATTCACAGGA CGGGAAACCT CCAAGAAAAC CAGACAGAAG
    AGCAGCCCCC GGAAGCCCGA ACCTCTCAGC CCTGGGAAGT CAGCCAGGGA TGCCTCACCA
    AGAGACCTGA CAACCAAGCC TAGCCGGCCG TCCACCCCAG CCCTGGTCCT TGCCCCAGCT
    TACCTCCTGT CCTCCAGCCC CCAGCCCACC AGCAGCAGCT TCCCTTTCTT CCACCTGCTG
    GGCCCTACAC CTTTCCCGAT GCTGATGGGG CCTCCAGGCT CCAAGGGAGA CTGTGGCTTG
    CCGGGTCCCC CTGGGCTTCC AGGATTACCT GGATCTCCGG GCGCACGAGG TCCTCGGGGT
    CCTCCTGGGC CGTACGGGAA CCCAGGCCCA CCTGGCCCTC CAGGAGCCAA AGGACAGAAA
    GGGGACCCAG GACTCTCACC AGGACAGGCT CACGATGGAG CAAAGGGCAA CATGGGCTTG
    CCTGGGCTCT CCGGAAATCC AGGACCCTTG GGAAGGAAGG GACACAAGGG CCATCCTGGA
    GCAGCAGGGC ACCCTGGAGA ACAGGGGCAG CCAGGACCTG AGGGCAGTCC AGGGGCCAAA
    GGTTACCCTG GCAGGCAGGG GTTCCCTGGA CCTGTAGGAG ACCCTGGCCC CAAAGGCAGC
    AGGGGTTACA TTGGTCTTCC GGGGCTCTTT GGCCTGCCAG GGTCGGATGG AGAGCGGGGT
    CTCCCTGGCG TTCCTGGCAA GAGGGGCGAG ATGGGTAGGC CGGGCTTCCC TGGAGACTTT
    GGGGAAAGAG GCCCCCCTGG ACTTGATGGC AACCCTGGAG AAATTGGCCT GCCGGGGCCA
    CCAGGAGTGC TCGGGCTCAT TGGTGACACA GGAGCACTGG GCCCAGTTGG CTACCCAGGA
    CCGAAAGGCA TGAAGGGACT GATGGGCGGC GTGGGGGAGC CCGGACTGAA AGGTGATAAG
    GGTGAACAAG GGGTGCCAGG TGTGTCGGGA GATCCTGGCT TCCAAGGAGA CAAGGGGAGT
    CATGGCTTGC CAGGGCTCCC GGGTGGCCGG GGGAAGCCAG GGCCTCTGGG AAAAGCTGGA
    GACAAAGGAT CACTTGGGTT TCCTGGACCT CCTGGACCTG AGGGTTTCCC AGGAGACATT
    GGCCCTCCAG GGGACAATGG CCCTGAAGGC ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG
    CCTGGACCTC CTGGGCAGCT GGGGCCTGAG GGAGATGAAG GACCCATGGG GCCACCAGGA
    GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT
    GGCTCGAAGG GGGAACCAGG GGATCCTGGA CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT
    ATGGGATTCA TTGGTCTTGT CGGAGAGCCT GGAATTGTTG GGGAGAAGGG AGACCGTGGG
    GTGATGGGAC CCCCAGGTGC ACCTGGACCC AAGGGGTCGA TGGGCCATCC TGGAACACCC
    GGTGGTATTG GGAACCCTGG AGAGCCTGGA CCCTGGGGCC CTCCAGGATC TCGAGGCCTG
    CCAGGCATGA GGGGAGCCAA GGGGCACCGG GGCCCTCGAG GACCTGATGG ACCAGCTGGG
    GAGCAGGGAT CCAAGGGCCT GAAGGGTCGT GTAGGACCTC GGGGCAGACC TGGCCAGCCA
    GGACAGCAGG GTGCAGCTGG TGAGCGAGGC CACTCAGGTG CAAAAGGCTT CCTTGGCATC
    CCTGGTCCCT CAGGCCCTCC AGGTGCCAAG GGCCTCCCAG GAGAACCGGG CTCTCAGGGA
    CCTCAGGGGC CAGTTGGTCC TCCAGGAGAA ATGGGGCCCA AGGGACCGCC GGGTGCAGTG
    GGAGAGCCTG GCCTTCCTGG GGACTCCGGA ATGAAGGGTG ACCTCGGACC TCTGGGCCCC
    CCTGGGGAGC AAGGTCTTAT TGGGCAGCGG GGAGAGCCAG GCCTTGAGGG AGACCATGGC
    CCTGTGGGGC CAGATGGGCT GAAGGGCGAC AGGGGCGACC CAGGGCCCGA TGGCGAGCAT
    GGTGAAAAGG GCCAGGAAGG GCTGAAAGGA GAGGATGGGT CCCCTGGGCC CCCGGGCATC
    ACTGGCGTCC CGGGTCGTGA GGGGAAGCCG GGCAAGCAGG GTGAGAAAGG CCAGAGGGGA
    GCTAAGGGTG CCAAGGGCCA CCAAGGATAT CTGGGAGAGA TGGGCATCCC CGGAGAACCT
    GGGCCCCCTG GGACCCCAGG TCCTAAAGGG TCCCGGGGCA CCCTAGGACC AACGGGTGCT
    CCAGGACGGA TGGGAGCCCA AGGAGAACCA GGATTGGCCG GTTATAATGG ACACAAAGGC
    ATCACAGGAC CCCTCGGGCC TCCCGGGCCC AAAGGCGAGA AGGGGGACCA GGGCGAGGAT
    GGCAAGACCG AGGGGCCCCC TGGGCCACCA GGAGATCGGG GTCCTGTGGG TGATCGAGGA
    GACCGTGGGG AGCCAGGCGA CCCTGGATAC CCTGGTCAAG AGGGAGTTCA AGGCCTCCGT
    GGAGAACCGG GCCAGCAGGG ACAGCCTGGG CATCCAGGAC CCCGGGGGCG CCCAGGACCC
    AAAGGATCAA AAGGCGAAGA GGGCCCAAAG GGAAAGCCAG GCAAGGCTGG GCCATCAGGC
    CGGAGGGGGA CCCAGGGCCT TCAAGGACTG CCAGGCCCCC GAGGCGTGGT GGGGAGACAG
    GGCCCTGAGG GCACTGCTGG ATCTGATGGG ATTCCTGGCA GAGATGGTCG GCCAGGATAT
    CAGGGAGACC AGGGAAATGA TGGGGACCCT GGCCCTGTGG GCCCTGCTGG GAGAAGAGGA
    AATCCAGGTG TGGCTGGCTT GCCTGGAGCA CAGGGGCCTC CGGGATTCAA GGGTGAAAGT
    GGGTTACCTG GGCAACTGGG TCCCCCTGGA AAACGAGGGA CAGAAGGTGG AACAGGGCTT
    CCTGGGAACC AGGGGGAGCC AGGATCCAAA GGCCAGCCGG GTGACTCTGG CGAGATGGGC
    TTCCCAGGAG TGGCTGGCCT CTTTGGACCC AAGGGCCCCC CTGGAGACAT TGGCTTCAAA
    GGCATCCAAG GTCCTCGGGG GCCTCCTGGC TTGATGGCCC AGATCGAGGA CTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030109491.1
    CDS509..5185
    Translation

    Target ORF information:

    RefSeq Version XM_030253631.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030253631.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGGCACGG GATTCGCGCG GGGGGCCCGA GGCACAGCGG CGTCAGGACC CGGGGGGGGG 
    TTTCTCTTCG CCTGGATCTT GGTCTCATTT ACCTGTCACC TGGCCTCCAC CCAAGGAGCT
    CCTGAAGATG TGGATGTCCT CCAGCGGCTG GGCCTCAGCT GGACGAAGGC CGGGGGTGGC
    CGGAGTCCTA CACCCCCTGG TGTCATTCCT TTCCCATCTG GCTTCATCTT CACACAGCGG
    GCCAAGCTGC AGGCCCCCAC AGCCAATGTT CTTCCCACCA CCCTGGGGCG GGAGCTGGCA
    TTGGTGTTGA GCCTCTGTTC ACATCGTGTG AACCATGCCT TCCTCTTTGC CATCCGCAGC
    AGGAAACACA AGCTGCAACT GGGTCTGCAG TTCCTCCCCG GCAGGACGAT CATCCACCTG
    GGGCCTCGGC AATCAGTGGC CTTCGACTTG GATGTGCATG ATGGGCGCTG GCACCACCTG
    GCCCTAGAGT TGCGTGGCCG CACAGTCACA ATGGTGACAG CCTGTGGGCA GCACAGGGTA
    CCTGTCCCAC TGCCTTCCCG TAGGGACTCC ATGCTTGACC CCCAGGGCTC CTTTCTCTTG
    GGGAAGGTGA ACCCCCGTGC TGTCCAGTTT GAAGGTGCAC TCTGTCAGTT CAGCATCCAC
    CCTGTGGCGC AGGTCGCTCA CAATTACTGT GCCCACCTGA GAGAGCGGTG CCGACAGGTG
    GACACATATA GTCCCCAGGT GGGAACCCTC TTTCCTTGGG ACTCTGGCCC AGCCTTTGCT
    TTGCATCCTG AACCAGCCTT GCTTGGCTTG GGGAATCTGA CCAGAACTCC AGCAACCCTG
    GGGGCCAGGC CTGTAAGCAG GGCACTTGCA GTGACTCTGG CTCCTGCCAT GCCCACCAAG
    CCCCTGAGGA CTGTCCACCC AGATGTCTCT GAACACAGCT CTTCCCAGAC CCCACTGTCA
    CCTGCCAAAC AGTCGGCCAG GAAAACACCT TCCCCCTCCT CGTCAGCTTC CCTGGCTAAT
    TCCACCAGGG TTTATCGTCC AGCTGCTGCC CAGCCAAGAC AGATCACAAC TACTAGCCCC
    ACCAAACGTT CCCCCACCAA GCCTTCTGTT TCTCCCCTTT CAGTTACCCC CATGAAAAGT
    CCCCATGCAA CCCAGAAAAC AGGTGTCCCT TCATTCACAA AGCCTGTTCC ACCCACTCAG
    AAGCCAGCGC CCTTCACCTC CTACCTAGCA CCTTCCAAAG CCTCTAGTCC CACTGTGAGG
    CCTGTTCAGA AGACTTTCAT GACACCCCGA CCTCCAGTCC CCAGCCCCCA GCCCCTGCGC
    CCTACTACTG GCTTATCTAA GAAGTTCACT AACCCCACTG TTGCAAAATC TAAGTCCAAG
    ACGACCAGTT GGGCCAGCAA GCCGGTCTTG GCCCGCTCCA GTGTCCCTAA AACGCTTCAG
    CAAACTGTTT TGTCCCAGTC TCCTGTTTCC TATCTGGGCT CTCAGACACT GGCTCCAGCC
    CTCCCTCCTT TGGGTGTTGG AAATCCTAGA ACGATGCCTC CCACACGTGA CTCTGCTTTA
    ACTCCTGCTG GAAGCAAGAA ATTCACAGGA CGGGAAACCT CCAAGAAAAC CAGACAGAAG
    AGCAGCCCCC GGAAGCCCGA ACCTCTCAGC CCTGGGAAGT CAGCCAGGGA TGCCTCACCA
    AGAGACCTGA CAACCAAGCC TAGCCGGCCG TCCACCCCAG CCCTGGTCCT TGCCCCAGCT
    TACCTCCTGT CCTCCAGCCC CCAGCCCACC AGCAGCAGCT TCCCTTTCTT CCACCTGCTG
    GGCCCTACAC CTTTCCCGAT GCTGATGGGG CCTCCAGGCT CCAAGGGAGA CTGTGGCTTG
    CCGGGTCCCC CTGGGCTTCC AGGATTACCT GGATCTCCGG GCGCACGAGG TCCTCGGGGT
    CCTCCTGGGC CGTACGGGAA CCCAGGCCCA CCTGGCCCTC CAGGAGCCAA AGGACAGAAA
    GGGGACCCAG GACTCTCACC AGGACAGGCT CACGATGGAG CAAAGGGCAA CATGGGCTTG
    CCTGGGCTCT CCGGAAATCC AGGACCCTTG GGAAGGAAGG GACACAAGGG CCATCCTGGA
    GCAGCAGGGC ACCCTGGAGA ACAGGGGCAG CCAGGACCTG AGGGCAGTCC AGGGGCCAAA
    GGTTACCCTG GCAGGCAGGG GTTCCCTGGA CCTGTAGGAG ACCCTGGCCC CAAAGGCAGC
    AGGGGTTACA TTGGTCTTCC GGGGCTCTTT GGCCTGCCAG GGTCGGATGG AGAGCGGGGT
    CTCCCTGGCG TTCCTGGCAA GAGGGGCGAG ATGGGTAGGC CGGGCTTCCC TGGAGACTTT
    GGGGAAAGAG GCCCCCCTGG ACTTGATGGC AACCCTGGAG AAATTGGCCT GCCGGGGCCA
    CCAGGAGTGC TCGGGCTCAT TGGTGACACA GGAGCACTGG GCCCAGTTGG CTACCCAGGA
    CCGAAAGGCA TGAAGGGACT GATGGGCGGC GTGGGGGAGC CCGGACTGAA AGGTGATAAG
    GGTGAACAAG GGGTGCCAGG TGTGTCGGGA GATCCTGGCT TCCAAGGAGA CAAGGGGAGT
    CATGGCTTGC CAGGGCTCCC GGGTGGCCGG GGGAAGCCAG GGCCTCTGGG AAAAGCTGGA
    GACAAAGGAT CACTTGGGTT TCCTGGACCT CCTGGACCTG AGGGTTTCCC AGGAGACATT
    GGCCCTCCAG GGGACAATGG CCCTGAAGGC ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG
    CCTGGACCTC CTGGGCAGCT GGGGCCTGAG GGAGATGAAG GACCCATGGG GCCACCAGGA
    GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT
    GGCTCGAAGG GGGAACCAGG GGATCCTGGA CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT
    ATGGGATTCA TTGGTCTTGT CGGAGAGCCT GGAATTGTTG GGGAGAAGGG AGACCGTGGG
    GTGATGGGAC CCCCAGGTGC ACCTGGACCC AAGGGGTCGA TGGGCCATCC TGGAACACCC
    GGTGGTATTG GGAACCCTGG AGAGCCTGGA CCCTGGGGCC CTCCAGGATC TCGAGGCCTG
    CCAGGCATGA GGGGAGCCAA GGGGCACCGG GGCCCTCGAG GACCTGATGG ACCAGCTGGG
    GAGCAGGGAT CCAAGGGCCT GAAGGGTCGT GTAGGACCTC GGGGCAGACC TGGCCAGCCA
    GGACAGCAGG GTGCAGCTGG TGAGCGAGGC CACTCAGGTG CAAAAGGCTT CCTTGGCATC
    CCTGGTCCCT CAGGCCCTCC AGGTGCCAAG GGCCTCCCAG GAGAACCGGG CTCTCAGGGA
    CCTCAGGGGC CAGTTGGTCC TCCAGGAGAA ATGGGGCCCA AGGGACCGCC GGGTGCAGTG
    GGAGAGCCTG GCCTTCCTGG GGACTCCGGA ATGAAGGGTG ACCTCGGACC TCTGGGCCCC
    CCTGGGGAGC AAGGTCTTAT TGGGCAGCGG GGAGAGCCAG GCCTTGAGGG AGACCATGGC
    CCTGTGGGGC CAGATGGGCT GAAGGGCGAC AGGGGCGACC CAGGGCCCGA TGGCGAGCAT
    GGTGAAAAGG GCCAGGAAGG GCTGAAAGGA GAGGATGGGT CCCCTGGGCC CCCGGGCATC
    ACTGGCGTCC CGGGTCGTGA GGGGAAGCCG GGCAAGCAGG GTGAGAAAGG CCAGAGGGGA
    GCTAAGGGTG CCAAGGGCCA CCAAGGATAT CTGGGAGAGA TGGGCATCCC CGGAGAACCT
    GGGCCCCCTG GGACCCCAGG TCCTAAAGGG TCCCGGGGCA CCCTAGGACC AACGGGTGCT
    CCAGGACGGA TGGGAGCCCA AGGAGAACCA GGATTGGCCG GTTATAATGG ACACAAAGGC
    ATCACAGGAC CCCTCGGGCC TCCCGGGCCC AAAGGCGAGA AGGGGGACCA GGGCGAGGAT
    GGCAAGACCG AGGGGCCCCC TGGGCCACCA GGAGATCGGG GTCCTGTGGG TGATCGAGGA
    GACCGTGGGG AGCCAGGCGA CCCTGGATAC CCTGGTCAAG AGGGAGTTCA AGGCCTCCGT
    GGAGAACCGG GCCAGCAGGG ACAGCCTGGG CATCCAGGAC CCCGGGGGCG CCCAGGACCC
    AAAGGATCAA AAGGCGAAGA GGGCCCAAAG GGAAAGCCAG GCAAGGCTGG GCCATCAGGC
    CGGAGGGGGA CCCAGGGCCT TCAAGGACTG CCAGGCCCCC GAGGCGTGGT GGGGAGACAG
    GGCCCTGAGG GCACTGCTGG ATCTGATGGG ATTCCTGGCA GAGATGGTCG GCCAGGATAT
    CAGGGAGACC AGGGAAATGA TGGGGACCCT GGCCCTGTGG GCCCTGCTGG GAGAAGAGGA
    AATCCAGGTG TGGCTGGCTT GCCTGGAGCA CAGGGGCCTC CGGGATTCAA GGGTGAAAGT
    GGGTTACCTG GGCAACTGGG TCCCCCTGGA AAACGAGGGA CAGAAGGTGG AACAGGGCTT
    CCTGGGAACC AGGGGGAGCC AGGATCCAAA GGCCAGCCGG GTGACTCTGG CGAGATGGGC
    TTCCCAGGAG TGGCTGGCCT CTTTGGACCC AAGGGCCCCC CTGGAGACAT TGGCTTCAAA
    GGCATCCAAG GTCCTCGGGG GCCTCCTGGC TTGATGGCCC AGATCGAGGA CTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu20706
    Clone ID Related Accession (Same CDS sequence) NM_025685.3
    Accession Version NM_025685.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5538bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-26
    Organism Mus musculus(house mouse)
    Product collagen alpha-1(XXVII) chain preproprotein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK157951.1, AL691496.13, AY167568.2 and AL683828.8. On Oct 12, 2006 this sequence version replaced NM_025685.2. ##Evidence-Data-START## Transcript exon combination :: AY167568.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849382 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGGCACGG GATTCGCGCG GGGGGCCCGA GGCACAGCGG CGTCAGGACC CGGGGGGGGG 
    TTTCTCTTCG CCTGGATCTT GGTCTCATTT ACCTGTCACC TGGCCTCCAC CCAAGGAGCT
    CCTGAAGATG TGGATGTCCT CCAGCGGCTG GGCCTCAGCT GGACGAAGGC CGGGGGTGGC
    CGGAGTCCTA CACCCCCTGG TGTCATTCCT TTCCCATCTG GCTTCATCTT CACACAGCGG
    GCCAAGCTGC AGGCCCCCAC AGCCAATGTT CTTCCCACCA CCCTGGGGCG GGAGCTGGCA
    TTGGTGTTGA GCCTCTGTTC ACATCGTGTG AACCATGCCT TCCTCTTTGC CATCCGCAGC
    AGGAAACACA AGCTGCAACT GGGTCTGCAG TTCCTCCCCG GCAGGACGAT CATCCACCTG
    GGGCCTCGGC AATCAGTGGC CTTCGACTTG GATGTGCATG ATGGGCGCTG GCACCACCTG
    GCCCTAGAGT TGCGTGGCCG CACAGTCACA ATGGTGACAG CCTGTGGGCA GCACAGGGTA
    CCTGTCCCAC TGCCTTCCCG TAGGGACTCC ATGCTTGACC CCCAGGGCTC CTTTCTCTTG
    GGGAAGGTGA ACCCCCGTGC TGTCCAGTTT GAAGGTGCAC TCTGTCAGTT CAGCATCCAC
    CCTGTGGCGC AGGTCGCTCA CAATTACTGT GCCCACCTGA GAGAGCGGTG CCGACAGGTG
    GACACATATA GTCCCCAGGT GGGAACCCTC TTTCCTTGGG ACTCTGGCCC AGCCTTTGCT
    TTGCATCCTG AACCAGCCTT GCTTGGCTTG GGGAATCTGA CCAGAACTCC AGCAACCCTG
    GGGGCCAGGC CTGTAAGCAG GGCACTTGCA GTGACTCTGG CTCCTGCCAT GCCCACCAAG
    CCCCTGAGGA CTGTCCACCC AGATGTCTCT GAACACAGCT CTTCCCAGAC CCCACTGTCA
    CCTGCCAAAC AGTCGGCCAG GAAAACACCT TCCCCCTCCT CGTCAGCTTC CCTGGCTAAT
    TCCACCAGGG TTTATCGTCC AGCTGCTGCC CAGCCAAGAC AGATCACAAC TACTAGCCCC
    ACCAAACGTT CCCCCACCAA GCCTTCTGTT TCTCCCCTTT CAGTTACCCC CATGAAAAGT
    CCCCATGCAA CCCAGAAAAC AGGTGTCCCT TCATTCACAA AGCCTGTTCC ACCCACTCAG
    AAGCCAGCGC CCTTCACCTC CTACCTAGCA CCTTCCAAAG CCTCTAGTCC CACTGTGAGG
    CCTGTTCAGA AGACTTTCAT GACACCCCGA CCTCCAGTCC CCAGCCCCCA GCCCCTGCGC
    CCTACTACTG GCTTATCTAA GAAGTTCACT AACCCCACTG TTGCAAAATC TAAGTCCAAG
    ACGACCAGTT GGGCCAGCAA GCCGGTCTTG GCCCGCTCCA GTGTCCCTAA AACGCTTCAG
    CAAACTGTTT TGTCCCAGTC TCCTGTTTCC TATCTGGGCT CTCAGACACT GGCTCCAGCC
    CTCCCTCCTT TGGGTGTTGG AAATCCTAGA ACGATGCCTC CCACACGTGA CTCTGCTTTA
    ACTCCTGCTG GAAGCAAGAA ATTCACAGGA CGGGAAACCT CCAAGAAAAC CAGACAGAAG
    AGCAGCCCCC GGAAGCCCGA ACCTCTCAGC CCTGGGAAGT CAGCCAGGGA TGCCTCACCA
    AGAGACCTGA CAACCAAGCC TAGCCGGCCG TCCACCCCAG CCCTGGTCCT TGCCCCAGCT
    TACCTCCTGT CCTCCAGCCC CCAGCCCACC AGCAGCAGCT TCCCTTTCTT CCACCTGCTG
    GGCCCTACAC CTTTCCCGAT GCTGATGGGG CCTCCAGGCT CCAAGGGAGA CTGTGGCTTG
    CCGGGTCCCC CTGGGCTTCC AGGATTACCT GGATCTCCGG GCGCACGAGG TCCTCGGGGT
    CCTCCTGGGC CGTACGGGAA CCCAGGCCCA CCTGGCCCTC CAGGAGCCAA AGGACAGAAA
    GGGGACCCAG GACTCTCACC AGGACAGGCT CACGATGGAG CAAAGGGCAA CATGGGCTTG
    CCTGGGCTCT CCGGAAATCC AGGACCCTTG GGAAGGAAGG GACACAAGGG CCATCCTGGA
    GCAGCAGGGC ACCCTGGAGA ACAGGGGCAG CCAGGACCTG AGGGCAGTCC AGGGGCCAAA
    GGTTACCCTG GCAGGCAGGG GTTCCCTGGA CCTGTAGGAG ACCCTGGCCC CAAAGGCAGC
    AGGGGTTACA TTGGTCTTCC GGGGCTCTTT GGCCTGCCAG GGTCGGATGG AGAGCGGGGT
    CTCCCTGGCG TTCCTGGCAA GAGGGGCGAG ATGGGTAGGC CGGGCTTCCC TGGAGACTTT
    GGGGAAAGAG GCCCCCCTGG ACTTGATGGC AACCCTGGAG AAATTGGCCT GCCGGGGCCA
    CCAGGAGTGC TCGGGCTCAT TGGTGACACA GGAGCACTGG GCCCAGTTGG CTACCCAGGA
    CCGAAAGGCA TGAAGGGACT GATGGGCGGC GTGGGGGAGC CCGGACTGAA AGGTGATAAG
    GGTGAACAAG GGGTGCCAGG TGTGTCGGGA GATCCTGGCT TCCAAGGAGA CAAGGGGAGT
    CATGGCTTGC CAGGGCTCCC GGGTGGCCGG GGGAAGCCAG GGCCTCTGGG AAAAGCTGGA
    GACAAAGGAT CACTTGGGTT TCCTGGACCT CCTGGACCTG AGGGTTTCCC AGGAGACATT
    GGCCCTCCAG GGGACAATGG CCCTGAAGGC ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG
    CCTGGACCTC CTGGGCAGCT GGGGCCTGAG GGAGATGAAG GACCCATGGG GCCACCAGGA
    GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT
    GGCTCGAAGG GGGAACCAGG GGATCCTGGA CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT
    ATGGGATTCA TTGGTCTTGT CGGAGAGCCT GGAATTGTTG GGGAGAAGGG AGACCGTGGG
    GTGATGGGAC CCCCAGGTGC ACCTGGACCC AAGGGGTCGA TGGGCCATCC TGGAACACCC
    GGTGGTATTG GGAACCCTGG AGAGCCTGGA CCCTGGGGCC CTCCAGGATC TCGAGGCCTG
    CCAGGCATGA GGGGAGCCAA GGGGCACCGG GGCCCTCGAG GACCTGATGG ACCAGCTGGG
    GAGCAGGGAT CCAAGGGCCT GAAGGGTCGT GTAGGACCTC GGGGCAGACC TGGCCAGCCA
    GGACAGCAGG GTGCAGCTGG TGAGCGAGGC CACTCAGGTG CAAAAGGCTT CCTTGGCATC
    CCTGGTCCCT CAGGCCCTCC AGGTGCCAAG GGCCTCCCAG GAGAACCGGG CTCTCAGGGA
    CCTCAGGGGC CAGTTGGTCC TCCAGGAGAA ATGGGGCCCA AGGGACCGCC GGGTGCAGTG
    GGAGAGCCTG GCCTTCCTGG GGACTCCGGA ATGAAGGGTG ACCTCGGACC TCTGGGCCCC
    CCTGGGGAGC AAGGTCTTAT TGGGCAGCGG GGAGAGCCAG GCCTTGAGGG AGACCATGGC
    CCTGTGGGGC CAGATGGGCT GAAGGGCGAC AGGGGCGACC CAGGGCCCGA TGGCGAGCAT
    GGTGAAAAGG GCCAGGAAGG GCTGAAAGGA GAGGATGGGT CCCCTGGGCC CCCGGGCATC
    ACTGGCGTCC CGGGTCGTGA GGGGAAGCCG GGCAAGCAGG GTGAGAAAGG CCAGAGGGGA
    GCTAAGGGTG CCAAGGGCCA CCAAGGATAT CTGGGAGAGA TGGGCATCCC CGGAGAACCT
    GGGCCCCCTG GGACCCCAGG TCCTAAAGGG TCCCGGGGCA CCCTAGGACC AACGGGTGCT
    CCAGGACGGA TGGGAGCCCA AGGAGAACCA GGATTGGCCG GTTATAATGG ACACAAAGGC
    ATCACAGGAC CCCTCGGGCC TCCCGGGCCC AAAGGCGAGA AGGGGGACCA GGGCGAGGAT
    GGCAAGACCG AGGGGCCCCC TGGGCCACCA GGAGATCGGG GTCCTGTGGG TGATCGAGGA
    GACCGTGGGG AGCCAGGCGA CCCTGGATAC CCTGGTCAAG AGGGAGTTCA AGGCCTCCGT
    GGAGAACCGG GCCAGCAGGG ACAGCCTGGG CATCCAGGAC CCCGGGGGCG CCCAGGACCC
    AAAGGATCAA AAGGCGAAGA GGGCCCAAAG GGAAAGCCAG GCAAGGCTGG GCCATCAGGC
    CGGAGGGGGA CCCAGGGCCT TCAAGGACTG CCAGGCCCCC GAGGCGTGGT GGGGAGACAG
    GGCCCTGAGG GCACTGCTGG ATCTGATGGG ATTCCTGGCA GAGATGGTCG GCCAGGATAT
    CAGGGAGACC AGGGAAATGA TGGGGACCCT GGCCCTGTGG GCCCTGCTGG GAGAAGAGGA
    AATCCAGGTG TGGCTGGCTT GCCTGGAGCA CAGGGGCCTC CGGGATTCAA GGGTGAAAGT
    GGGTTACCTG GGCAACTGGG TCCCCCTGGA AAACGAGGGA CAGAAGGTGG AACAGGGCTT
    CCTGGGAACC AGGGGGAGCC AGGATCCAAA GGCCAGCCGG GTGACTCTGG CGAGATGGGC
    TTCCCAGGAG TGGCTGGCCT CTTTGGACCC AAGGGCCCCC CTGGAGACAT TGGCTTCAAA
    GGCATCCAAG GTCCTCGGGG GCCTCCTGGC TTGATGGGAA AGGAAGGTAT CATTGGCCCC
    CCCGGAATGC TGGGACCTTC TGGACTCCCG GGTCCCAAAG GTGACAGAGG CAGCCGAGGA
    GACTTGGGAC TGCAAGGCCC AAGGGGTCCT CCTGGTCCAA GGGGCCGGCC AGGTCCCCCG
    GGCCCTCCTT GGCATCCCAT CCAGTTTCAG CAAGATGACC TTGGAGCAGC TTTCCAGACA
    TGGATGGATG CTCAAGGAGC CGTCAGATCG GAGGGGTACA GCTATCCGGA CCAGCTGGCG
    CTAGACCAGG GAGGGGAGAT CTTCAAAACC TTACACTACC TCAGCAACCT CATCCAGAGC
    ATTAAGACAC CCTTGGGCAC CAAGGAGAAC CCAGCCCGGG TCTGCCGGGA CCTCATGGAC
    TGTGAGCAGA GGATGGCGGA TGGTACCTAC TGGGTGGACC CCAACCTCGG CTGCTCCTCT
    GACACCATTG AAGTCTCCTG CAACTTTACA CAGGGTGGGC AGACGTGCCT GAAGCCCATC
    ACGGCCTCCA AGGCCGAGTT TGCTGTGAGT CGGGTCCAGA TGAATTTCTT GCACCTGCTG
    AGCTCTGAGG GGACACAGCA TATCACAATC CACTGTCTGA ACATGACGGT GTGGCAGGAG
    GGACCGGGAC GCTCCTCTGC CAGACAGGCT GTGCGCTTCC GTGCCTGGAA CGGACAGGTC
    TTCGAAGCTG GGGGTCAGTT CAGGCCAGAG GTGTCTATGG ATGGCTGCAA GGTCCATGAT
    GGCCGCTGGC ATCAGACACT GTTCACCTTC CGGACCCAGG ACCCCCAGCA GCTGCCCATC
    GTCAGTGTGG ACAATCTCCC GCCTGTCTCA TCAGGGAAGC AGTACCGCCT GGAAGTTGGA
    CCTGCGTGCT TCCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_079961.3
    CDS415..5952
    Translation

    Target ORF information:

    RefSeq Version NM_025685.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus collagen, type XXVII, alpha 1 (Col27a1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_025685.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGGCACGG GATTCGCGCG GGGGGCCCGA GGCACAGCGG CGTCAGGACC CGGGGGGGGG 
    TTTCTCTTCG CCTGGATCTT GGTCTCATTT ACCTGTCACC TGGCCTCCAC CCAAGGAGCT
    CCTGAAGATG TGGATGTCCT CCAGCGGCTG GGCCTCAGCT GGACGAAGGC CGGGGGTGGC
    CGGAGTCCTA CACCCCCTGG TGTCATTCCT TTCCCATCTG GCTTCATCTT CACACAGCGG
    GCCAAGCTGC AGGCCCCCAC AGCCAATGTT CTTCCCACCA CCCTGGGGCG GGAGCTGGCA
    TTGGTGTTGA GCCTCTGTTC ACATCGTGTG AACCATGCCT TCCTCTTTGC CATCCGCAGC
    AGGAAACACA AGCTGCAACT GGGTCTGCAG TTCCTCCCCG GCAGGACGAT CATCCACCTG
    GGGCCTCGGC AATCAGTGGC CTTCGACTTG GATGTGCATG ATGGGCGCTG GCACCACCTG
    GCCCTAGAGT TGCGTGGCCG CACAGTCACA ATGGTGACAG CCTGTGGGCA GCACAGGGTA
    CCTGTCCCAC TGCCTTCCCG TAGGGACTCC ATGCTTGACC CCCAGGGCTC CTTTCTCTTG
    GGGAAGGTGA ACCCCCGTGC TGTCCAGTTT GAAGGTGCAC TCTGTCAGTT CAGCATCCAC
    CCTGTGGCGC AGGTCGCTCA CAATTACTGT GCCCACCTGA GAGAGCGGTG CCGACAGGTG
    GACACATATA GTCCCCAGGT GGGAACCCTC TTTCCTTGGG ACTCTGGCCC AGCCTTTGCT
    TTGCATCCTG AACCAGCCTT GCTTGGCTTG GGGAATCTGA CCAGAACTCC AGCAACCCTG
    GGGGCCAGGC CTGTAAGCAG GGCACTTGCA GTGACTCTGG CTCCTGCCAT GCCCACCAAG
    CCCCTGAGGA CTGTCCACCC AGATGTCTCT GAACACAGCT CTTCCCAGAC CCCACTGTCA
    CCTGCCAAAC AGTCGGCCAG GAAAACACCT TCCCCCTCCT CGTCAGCTTC CCTGGCTAAT
    TCCACCAGGG TTTATCGTCC AGCTGCTGCC CAGCCAAGAC AGATCACAAC TACTAGCCCC
    ACCAAACGTT CCCCCACCAA GCCTTCTGTT TCTCCCCTTT CAGTTACCCC CATGAAAAGT
    CCCCATGCAA CCCAGAAAAC AGGTGTCCCT TCATTCACAA AGCCTGTTCC ACCCACTCAG
    AAGCCAGCGC CCTTCACCTC CTACCTAGCA CCTTCCAAAG CCTCTAGTCC CACTGTGAGG
    CCTGTTCAGA AGACTTTCAT GACACCCCGA CCTCCAGTCC CCAGCCCCCA GCCCCTGCGC
    CCTACTACTG GCTTATCTAA GAAGTTCACT AACCCCACTG TTGCAAAATC TAAGTCCAAG
    ACGACCAGTT GGGCCAGCAA GCCGGTCTTG GCCCGCTCCA GTGTCCCTAA AACGCTTCAG
    CAAACTGTTT TGTCCCAGTC TCCTGTTTCC TATCTGGGCT CTCAGACACT GGCTCCAGCC
    CTCCCTCCTT TGGGTGTTGG AAATCCTAGA ACGATGCCTC CCACACGTGA CTCTGCTTTA
    ACTCCTGCTG GAAGCAAGAA ATTCACAGGA CGGGAAACCT CCAAGAAAAC CAGACAGAAG
    AGCAGCCCCC GGAAGCCCGA ACCTCTCAGC CCTGGGAAGT CAGCCAGGGA TGCCTCACCA
    AGAGACCTGA CAACCAAGCC TAGCCGGCCG TCCACCCCAG CCCTGGTCCT TGCCCCAGCT
    TACCTCCTGT CCTCCAGCCC CCAGCCCACC AGCAGCAGCT TCCCTTTCTT CCACCTGCTG
    GGCCCTACAC CTTTCCCGAT GCTGATGGGG CCTCCAGGCT CCAAGGGAGA CTGTGGCTTG
    CCGGGTCCCC CTGGGCTTCC AGGATTACCT GGATCTCCGG GCGCACGAGG TCCTCGGGGT
    CCTCCTGGGC CGTACGGGAA CCCAGGCCCA CCTGGCCCTC CAGGAGCCAA AGGACAGAAA
    GGGGACCCAG GACTCTCACC AGGACAGGCT CACGATGGAG CAAAGGGCAA CATGGGCTTG
    CCTGGGCTCT CCGGAAATCC AGGACCCTTG GGAAGGAAGG GACACAAGGG CCATCCTGGA
    GCAGCAGGGC ACCCTGGAGA ACAGGGGCAG CCAGGACCTG AGGGCAGTCC AGGGGCCAAA
    GGTTACCCTG GCAGGCAGGG GTTCCCTGGA CCTGTAGGAG ACCCTGGCCC CAAAGGCAGC
    AGGGGTTACA TTGGTCTTCC GGGGCTCTTT GGCCTGCCAG GGTCGGATGG AGAGCGGGGT
    CTCCCTGGCG TTCCTGGCAA GAGGGGCGAG ATGGGTAGGC CGGGCTTCCC TGGAGACTTT
    GGGGAAAGAG GCCCCCCTGG ACTTGATGGC AACCCTGGAG AAATTGGCCT GCCGGGGCCA
    CCAGGAGTGC TCGGGCTCAT TGGTGACACA GGAGCACTGG GCCCAGTTGG CTACCCAGGA
    CCGAAAGGCA TGAAGGGACT GATGGGCGGC GTGGGGGAGC CCGGACTGAA AGGTGATAAG
    GGTGAACAAG GGGTGCCAGG TGTGTCGGGA GATCCTGGCT TCCAAGGAGA CAAGGGGAGT
    CATGGCTTGC CAGGGCTCCC GGGTGGCCGG GGGAAGCCAG GGCCTCTGGG AAAAGCTGGA
    GACAAAGGAT CACTTGGGTT TCCTGGACCT CCTGGACCTG AGGGTTTCCC AGGAGACATT
    GGCCCTCCAG GGGACAATGG CCCTGAAGGC ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG
    CCTGGACCTC CTGGGCAGCT GGGGCCTGAG GGAGATGAAG GACCCATGGG GCCACCAGGA
    GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT
    GGCTCGAAGG GGGAACCAGG GGATCCTGGA CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT
    ATGGGATTCA TTGGTCTTGT CGGAGAGCCT GGAATTGTTG GGGAGAAGGG AGACCGTGGG
    GTGATGGGAC CCCCAGGTGC ACCTGGACCC AAGGGGTCGA TGGGCCATCC TGGAACACCC
    GGTGGTATTG GGAACCCTGG AGAGCCTGGA CCCTGGGGCC CTCCAGGATC TCGAGGCCTG
    CCAGGCATGA GGGGAGCCAA GGGGCACCGG GGCCCTCGAG GACCTGATGG ACCAGCTGGG
    GAGCAGGGAT CCAAGGGCCT GAAGGGTCGT GTAGGACCTC GGGGCAGACC TGGCCAGCCA
    GGACAGCAGG GTGCAGCTGG TGAGCGAGGC CACTCAGGTG CAAAAGGCTT CCTTGGCATC
    CCTGGTCCCT CAGGCCCTCC AGGTGCCAAG GGCCTCCCAG GAGAACCGGG CTCTCAGGGA
    CCTCAGGGGC CAGTTGGTCC TCCAGGAGAA ATGGGGCCCA AGGGACCGCC GGGTGCAGTG
    GGAGAGCCTG GCCTTCCTGG GGACTCCGGA ATGAAGGGTG ACCTCGGACC TCTGGGCCCC
    CCTGGGGAGC AAGGTCTTAT TGGGCAGCGG GGAGAGCCAG GCCTTGAGGG AGACCATGGC
    CCTGTGGGGC CAGATGGGCT GAAGGGCGAC AGGGGCGACC CAGGGCCCGA TGGCGAGCAT
    GGTGAAAAGG GCCAGGAAGG GCTGAAAGGA GAGGATGGGT CCCCTGGGCC CCCGGGCATC
    ACTGGCGTCC CGGGTCGTGA GGGGAAGCCG GGCAAGCAGG GTGAGAAAGG CCAGAGGGGA
    GCTAAGGGTG CCAAGGGCCA CCAAGGATAT CTGGGAGAGA TGGGCATCCC CGGAGAACCT
    GGGCCCCCTG GGACCCCAGG TCCTAAAGGG TCCCGGGGCA CCCTAGGACC AACGGGTGCT
    CCAGGACGGA TGGGAGCCCA AGGAGAACCA GGATTGGCCG GTTATAATGG ACACAAAGGC
    ATCACAGGAC CCCTCGGGCC TCCCGGGCCC AAAGGCGAGA AGGGGGACCA GGGCGAGGAT
    GGCAAGACCG AGGGGCCCCC TGGGCCACCA GGAGATCGGG GTCCTGTGGG TGATCGAGGA
    GACCGTGGGG AGCCAGGCGA CCCTGGATAC CCTGGTCAAG AGGGAGTTCA AGGCCTCCGT
    GGAGAACCGG GCCAGCAGGG ACAGCCTGGG CATCCAGGAC CCCGGGGGCG CCCAGGACCC
    AAAGGATCAA AAGGCGAAGA GGGCCCAAAG GGAAAGCCAG GCAAGGCTGG GCCATCAGGC
    CGGAGGGGGA CCCAGGGCCT TCAAGGACTG CCAGGCCCCC GAGGCGTGGT GGGGAGACAG
    GGCCCTGAGG GCACTGCTGG ATCTGATGGG ATTCCTGGCA GAGATGGTCG GCCAGGATAT
    CAGGGAGACC AGGGAAATGA TGGGGACCCT GGCCCTGTGG GCCCTGCTGG GAGAAGAGGA
    AATCCAGGTG TGGCTGGCTT GCCTGGAGCA CAGGGGCCTC CGGGATTCAA GGGTGAAAGT
    GGGTTACCTG GGCAACTGGG TCCCCCTGGA AAACGAGGGA CAGAAGGTGG AACAGGGCTT
    CCTGGGAACC AGGGGGAGCC AGGATCCAAA GGCCAGCCGG GTGACTCTGG CGAGATGGGC
    TTCCCAGGAG TGGCTGGCCT CTTTGGACCC AAGGGCCCCC CTGGAGACAT TGGCTTCAAA
    GGCATCCAAG GTCCTCGGGG GCCTCCTGGC TTGATGGGAA AGGAAGGTAT CATTGGCCCC
    CCCGGAATGC TGGGACCTTC TGGACTCCCG GGTCCCAAAG GTGACAGAGG CAGCCGAGGA
    GACTTGGGAC TGCAAGGCCC AAGGGGTCCT CCTGGTCCAA GGGGCCGGCC AGGTCCCCCG
    GGCCCTCCTT GGCATCCCAT CCAGTTTCAG CAAGATGACC TTGGAGCAGC TTTCCAGACA
    TGGATGGATG CTCAAGGAGC CGTCAGATCG GAGGGGTACA GCTATCCGGA CCAGCTGGCG
    CTAGACCAGG GAGGGGAGAT CTTCAAAACC TTACACTACC TCAGCAACCT CATCCAGAGC
    ATTAAGACAC CCTTGGGCAC CAAGGAGAAC CCAGCCCGGG TCTGCCGGGA CCTCATGGAC
    TGTGAGCAGA GGATGGCGGA TGGTACCTAC TGGGTGGACC CCAACCTCGG CTGCTCCTCT
    GACACCATTG AAGTCTCCTG CAACTTTACA CAGGGTGGGC AGACGTGCCT GAAGCCCATC
    ACGGCCTCCA AGGCCGAGTT TGCTGTGAGT CGGGTCCAGA TGAATTTCTT GCACCTGCTG
    AGCTCTGAGG GGACACAGCA TATCACAATC CACTGTCTGA ACATGACGGT GTGGCAGGAG
    GGACCGGGAC GCTCCTCTGC CAGACAGGCT GTGCGCTTCC GTGCCTGGAA CGGACAGGTC
    TTCGAAGCTG GGGGTCAGTT CAGGCCAGAG GTGTCTATGG ATGGCTGCAA GGTCCATGAT
    GGCCGCTGGC ATCAGACACT GTTCACCTTC CGGACCCAGG ACCCCCAGCA GCTGCCCATC
    GTCAGTGTGG ACAATCTCCC GCCTGTCTCA TCAGGGAAGC AGTACCGCCT GGAAGTTGGA
    CCTGCGTGCT TCCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu33005
    Clone ID Related Accession (Same CDS sequence) XM_006538035.4 , XM_006538035.3
    Accession Version XM_006538035.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5541bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product collagen alpha-1(XXVII) chain isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006538035.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGGCACGG GATTCGCGCG GGGGGCCCGA GGCACAGCGG CGTCAGGACC CGGGGGGGGG 
    TTTCTCTTCG CCTGGATCTT GGTCTCATTT ACCTGTCACC TGGCCTCCAC CCAAGGAGCT
    CCTGAAGATG TGGATGTCCT CCAGCGGCTG GGCCTCAGCT GGACGAAGGC CGGGGGTGGC
    CGGAGTCCTA CACCCCCTGG TGTCATTCCT TTCCCATCTG GCTTCATCTT CACACAGCGG
    GCCAAGCTGC AGGCCCCCAC AGCCAATGTT CTTCCCACCA CCCTGGGGCG GGAGCTGGCA
    TTGGTGTTGA GCCTCTGTTC ACATCGTGTG AACCATGCCT TCCTCTTTGC CATCCGCAGC
    AGGAAACACA AGCTGCAACT GGGTCTGCAG TTCCTCCCCG GCAGGACGAT CATCCACCTG
    GGGCCTCGGC AATCAGTGGC CTTCGACTTG GATGTGCATG ATGGGCGCTG GCACCACCTG
    GCCCTAGAGT TGCGTGGCCG CACAGTCACA ATGGTGACAG CCTGTGGGCA GCACAGGGTA
    CCTGTCCCAC TGCCTTCCCG TAGGGACTCC ATGCTTGACC CCCAGGGCTC CTTTCTCTTG
    GGGAAGGTGA ACCCCCGTGC TGTCCAGTTT GAAGGTGCAC TCTGTCAGTT CAGCATCCAC
    CCTGTGGCGC AGGTCGCTCA CAATTACTGT GCCCACCTGA GAGAGCGGTG CCGACAGGTG
    GACACATATA GTCCCCAGGT GGGAACCCTC TTTCCTTGGG ACTCTGGCCC AGCCTTTGCT
    TTGCATCCTG AACCAGCCTT GCTTGGCTTG GGGAATCTGA CCAGAACTCC AGCAACCCTG
    GGGGCCAGGC CTGTAAGCAG GGCACTTGCA GTGACTCTGG CTCCTGCCAT GCCCACCAAG
    CCCCTGAGGA CTGTCCACCC AGATGTCTCT GAACACAGCT CTTCCCAGAC CCCACTGTCA
    CCTGCCAAAC AGTCGGCCAG GAAAACACCT TCCCCCTCCT CGTCAGCTTC CCTGGCTAAT
    TCCACCAGGG TTTATCGTCC AGCTGCTGCC CAGCCAAGAC AGATCACAAC TACTAGCCCC
    ACCAAACGTT CCCCCACCAA GCCTTCTGTT TCTCCCCTTT CAGTTACCCC CATGAAAAGT
    CCCCATGCAA CCCAGAAAAC AGGTGTCCCT TCATTCACAA AGCCTGTTCC ACCCACTCAG
    AAGCCAGCGC CCTTCACCTC CTACCTAGCA CCTTCCAAAG CCTCTAGTCC CACTGTGAGG
    CCTGTTCAGA AGACTTTCAT GACACCCCGA CCTCCAGTCC CCAGCCCCCA GCCCCTGCGC
    CCTACTACTG GCTTATCTAA GAAGTTCACT AACCCCACTG TTGCAAAATC TAAGTCCAAG
    ACGACCAGTT GGGCCAGCAA GCCGGTCTTG GCCCGCTCCA GTGTCCCTAA AACGCTTCAG
    CAAACTGTTT TGTCCCAGTC TCCTGTTTCC TATCTGGGCT CTCAGACACT GGCTCCAGCC
    CTCCCTCCTT TGGGTGTTGG AAATCCTAGA ACGATGCCTC CCACACGTGA CTCTGCTTTA
    ACTCCTGCTG GAAGCAAGAA ATTCACAGGA CGGGAAACCT CCAAGAAAAC CAGACAGAAG
    AGCAGCCCCC GGAAGCCCGA ACCTCTCAGC CCTGGGAAGT CAGCCAGGGA TGCCTCACCA
    AGAGACCTGA CAACCAAGCC TAGCCGGCCG TCCACCCCAG CCCTGGTCCT TGCCCCAGCT
    TACCTCCTGT CCTCCAGCCC CCAGCCCACC AGCAGCAGCT TCCCTTTCTT CCACCTGCTG
    GGCCCTACAC CTTTCCCGAT GCTGATGGGG CCTCCAGGCT CCAAGGGAGA CTGTGGCTTG
    CCGGGTCCCC CTGGGCTTCC AGGATTACCT GGATCTCCGG GCGCACGAGG TCCTCGGGGT
    CCTCCTGGGC CGTACGGGAA CCCAGGCCCA CCTGGCCCTC CAGGAGCCAA AGGACAGAAA
    GGGGACCCAG GACTCTCACC AGGACAGGCT CACGATGGAG CAAAGGGCAA CATGGGCTTG
    CCTGGGCTCT CCGGAAATCC AGGACCCTTG GGAAGGAAGG GACACAAGGG CCATCCTGGA
    GCAGCAGGGC ACCCTGGAGA ACAGGGGCAG CCAGGACCTG AGGGCAGTCC AGGGGCCAAA
    GGTTACCCTG GCAGGCAGGG GTTCCCTGGA CCTGTAGGAG ACCCTGGCCC CAAAGGCAGC
    AGGGGTTACA TTGGTCTTCC GGGGCTCTTT GGCCTGCCAG GGTCGGATGG AGAGCGGGGT
    CTCCCTGGCG TTCCTGGCAA GAGGGGCGAG ATGGGTAGGC CGGGCTTCCC TGGAGACTTT
    GGGGAAAGAG GCCCCCCTGG ACTTGATGGC AACCCTGGAG AAATTGGCCT GCCGGGGCCA
    CCAGGAGTGC TCGGGCTCAT TGGTGACACA GGAGCACTGG GCCCAGTTGG CTACCCAGGA
    CCGAAAGGCA TGAAGGGACT GATGGGCGGC GTGGGGGAGC CCGGACTGAA AGGTGATAAG
    GGTGAACAAG GGGTGCCAGG TGTGTCGGGA GATCCTGGCT TCCAAGGAGA CAAGGGGAGT
    CATGGCTTGC CAGGGCTCCC GGGTGGCCGG GGGAAGCCAG GGCCTCTGGG AAAAGCTGGA
    GACAAAGGAT CACTTGGGTT TCCTGGACCT CCTGGACCTG AGGGTTTCCC AGGAGACATT
    GGCCCTCCAG GGGACAATGG CCCTGAAGGC ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG
    CCTGGACCTC CTGGGCAGCT GGGGCCTGAG GGAGATGAAG GACCCATGGG GCCACCAGGA
    GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT
    GGCTCGAAGG GGGAACCAGG GGATCCTGGA CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT
    ATGGGATTCA TTGGTCTTGT CGGAGAGCCT GGAATTGTTG GGGAGAAGGG AGACCGTGGG
    GTGATGGGAC CCCCAGGTGC ACCTGGACCC AAGGGGTCGA TGGGCCATCC TGGAACACCC
    GGTGGTATTG GGAACCCTGG AGAGCCTGGA CCCTGGGGCC CTCCAGGATC TCGAGGCCTG
    CCAGGCATGA GGGGAGCCAA GGGGCACCGG GGCCCTCGAG GACCTGATGG ACCAGCTGGG
    GAGCAGGGAT CCAAGGGCCT GAAGGGTCGT GTAGGACCTC GGGGCAGACC TGGCCAGCCA
    GGACAGCAGG GTGCAGCTGG TGAGCGAGGC CACTCAGGTG CAAAAGGCTT CCTTGGCATC
    CCTGGTCCCT CAGGCCCTCC AGGTGCCAAG GGCCTCCCAG GAGAACCGGG CTCTCAGGGA
    CCTCAGGGGC CAGTTGGTCC TCCAGGAGAA ATGGGGCCCA AGGGACCGCC GGGTGCAGTG
    GGAGAGCCTG GCCTTCCTGG GGACTCCGGA ATGAAGGGTG ACCTCGGACC TCTGGGCCCC
    CCTGGGGAGC AAGGTCTTAT TGGGCAGCGG GGAGAGCCAG GCCTTGAGGG AGACCATGGC
    CCTGTGGGGC CAGATGGGCT GAAGGGCGAC AGGGGCGACC CAGGGCCCGA TGGCGAGCAT
    GGTGAAAAGG GCCAGGAAGG GCTGAAAGGA GAGGATGGGT CCCCTGGGCC CCCGGGCATC
    ACTGGCGTCC CGGGTCGTGA GGGGAAGCCG GGCAAGCAGG GTGAGAAAGG CCAGAGGGGA
    GCTAAGGGTG CCAAGGGCCA CCAAGGATAT CTGGGAGAGA TGGGCATCCC CGGAGAACCT
    GGGCCCCCTG GGACCCCAGG TCCTAAAGGG TCCCGGGGCA CCCTAGGACC AACGGGTGCT
    CCAGGACGGA TGGGAGCCCA AGGAGAACCA GGATTGGCCG GTTATAATGG ACACAAAGGC
    ATCACAGGAC CCCTCGGGCC TCCCGGGCCC AAAGGCGAGA AGGGGGACCA GGGCGAGGAT
    GGCAAGACCG AGGGGCCCCC TGGGCCACCA GGAGATCGGG GTCCTGTGGG TGATCGAGGA
    GACCGTGGGG AGCCAGGCGA CCCTGGATAC CCTGGTCAAG AGGGAGTTCA AGGCCTCCGT
    GGAGAACCGG GCCAGCAGGG ACAGCCTGGG CATCCAGGAC CCCGGGGGCG CCCAGGACCC
    AAAGGATCAA AAGGCGAAGA GGGCCCAAAG GGAAAGCCAG GCAAGGCTGG GCCATCAGGC
    CGGAGGGGGA CCCAGGGCCT TCAAGGACTG CCAGGCCCCC GAGGCGTGGT GGGGAGACAG
    GGCCCTGAGG GCACTGCTGG ATCTGATGGG ATTCCTGGCA GAGATGGTCG GCCAGGATAT
    CAGGGAGACC AGGGAAATGA TGGGGACCCT GGCCCTGTGG GCCCTGCTGG GAGAAGAGGA
    AATCCAGGTG TGGCTGGCTT GCCTGGAGCA CAGGGGCCTC CGGGATTCAA GGGTGAAAGT
    GGGTTACCTG GGCAACTGGG TCCCCCTGGA AAACGAGGGA CAGAAGGTGG AACAGGGCTT
    CCTGGGAACC AGGGGGAGCC AGGATCCAAA GGCCAGCCGG GTGACTCTGG CGAGATGGGC
    TTCCCAGGAG TGGCTGGCCT CTTTGGACCC AAGGGCCCCC CTGGAGACAT TGGCTTCAAA
    GGCATCCAAG GTCCTCGGGG GCCTCCTGGC TTGATGGGAA AGGAAGGTAT CATTGGCCCC
    CCCGGAATGC TGGGACCTTC TGGACTCCCG GGTCCCAAAG GTGACAGAGG CAGCCGAGGA
    GACTTGGGAC TGCAAGGCCC AAGGGGTCCT CCTGGTCCAA GGGGCCGGCC AGGTCCCCCG
    GGCCCTCCTT GGCATCCCAT CCAGTTTCAG CAAGATGACC TTGGAGCAGC TTTCCAGACA
    TGGATGGATG CTCAAGGAGC CGTCAGATCG GAGCAGGGGT ACAGCTATCC GGACCAGCTG
    GCGCTAGACC AGGGAGGGGA GATCTTCAAA ACCTTACACT ACCTCAGCAA CCTCATCCAG
    AGCATTAAGA CACCCTTGGG CACCAAGGAG AACCCAGCCC GGGTCTGCCG GGACCTCATG
    GACTGTGAGC AGAGGATGGC GGATGGTACC TACTGGGTGG ACCCCAACCT CGGCTGCTCC
    TCTGACACCA TTGAAGTCTC CTGCAACTTT ACACAGGGTG GGCAGACGTG CCTGAAGCCC
    ATCACGGCCT CCAAGGCCGA GTTTGCTGTG AGTCGGGTCC AGATGAATTT CTTGCACCTG
    CTGAGCTCTG AGGGGACACA GCATATCACA ATCCACTGTC TGAACATGAC GGTGTGGCAG
    GAGGGACCGG GACGCTCCTC TGCCAGACAG GCTGTGCGCT TCCGTGCCTG GAACGGACAG
    GTCTTCGAAG CTGGGGGTCA GTTCAGGCCA GAGGTGTCTA TGGATGGCTG CAAGGTCCAT
    GATGGCCGCT GGCATCAGAC ACTGTTCACC TTCCGGACCC AGGACCCCCA GCAGCTGCCC
    ATCGTCAGTG TGGACAATCT CCCGCCTGTC TCATCAGGGA AGCAGTACCG CCTGGAAGTT
    GGACCTGCGT GCTTCCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006538098.1
    CDS506..6046
    Translation

    Target ORF information:

    RefSeq Version XM_006538035.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006538035.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGGCACGG GATTCGCGCG GGGGGCCCGA GGCACAGCGG CGTCAGGACC CGGGGGGGGG 
    TTTCTCTTCG CCTGGATCTT GGTCTCATTT ACCTGTCACC TGGCCTCCAC CCAAGGAGCT
    CCTGAAGATG TGGATGTCCT CCAGCGGCTG GGCCTCAGCT GGACGAAGGC CGGGGGTGGC
    CGGAGTCCTA CACCCCCTGG TGTCATTCCT TTCCCATCTG GCTTCATCTT CACACAGCGG
    GCCAAGCTGC AGGCCCCCAC AGCCAATGTT CTTCCCACCA CCCTGGGGCG GGAGCTGGCA
    TTGGTGTTGA GCCTCTGTTC ACATCGTGTG AACCATGCCT TCCTCTTTGC CATCCGCAGC
    AGGAAACACA AGCTGCAACT GGGTCTGCAG TTCCTCCCCG GCAGGACGAT CATCCACCTG
    GGGCCTCGGC AATCAGTGGC CTTCGACTTG GATGTGCATG ATGGGCGCTG GCACCACCTG
    GCCCTAGAGT TGCGTGGCCG CACAGTCACA ATGGTGACAG CCTGTGGGCA GCACAGGGTA
    CCTGTCCCAC TGCCTTCCCG TAGGGACTCC ATGCTTGACC CCCAGGGCTC CTTTCTCTTG
    GGGAAGGTGA ACCCCCGTGC TGTCCAGTTT GAAGGTGCAC TCTGTCAGTT CAGCATCCAC
    CCTGTGGCGC AGGTCGCTCA CAATTACTGT GCCCACCTGA GAGAGCGGTG CCGACAGGTG
    GACACATATA GTCCCCAGGT GGGAACCCTC TTTCCTTGGG ACTCTGGCCC AGCCTTTGCT
    TTGCATCCTG AACCAGCCTT GCTTGGCTTG GGGAATCTGA CCAGAACTCC AGCAACCCTG
    GGGGCCAGGC CTGTAAGCAG GGCACTTGCA GTGACTCTGG CTCCTGCCAT GCCCACCAAG
    CCCCTGAGGA CTGTCCACCC AGATGTCTCT GAACACAGCT CTTCCCAGAC CCCACTGTCA
    CCTGCCAAAC AGTCGGCCAG GAAAACACCT TCCCCCTCCT CGTCAGCTTC CCTGGCTAAT
    TCCACCAGGG TTTATCGTCC AGCTGCTGCC CAGCCAAGAC AGATCACAAC TACTAGCCCC
    ACCAAACGTT CCCCCACCAA GCCTTCTGTT TCTCCCCTTT CAGTTACCCC CATGAAAAGT
    CCCCATGCAA CCCAGAAAAC AGGTGTCCCT TCATTCACAA AGCCTGTTCC ACCCACTCAG
    AAGCCAGCGC CCTTCACCTC CTACCTAGCA CCTTCCAAAG CCTCTAGTCC CACTGTGAGG
    CCTGTTCAGA AGACTTTCAT GACACCCCGA CCTCCAGTCC CCAGCCCCCA GCCCCTGCGC
    CCTACTACTG GCTTATCTAA GAAGTTCACT AACCCCACTG TTGCAAAATC TAAGTCCAAG
    ACGACCAGTT GGGCCAGCAA GCCGGTCTTG GCCCGCTCCA GTGTCCCTAA AACGCTTCAG
    CAAACTGTTT TGTCCCAGTC TCCTGTTTCC TATCTGGGCT CTCAGACACT GGCTCCAGCC
    CTCCCTCCTT TGGGTGTTGG AAATCCTAGA ACGATGCCTC CCACACGTGA CTCTGCTTTA
    ACTCCTGCTG GAAGCAAGAA ATTCACAGGA CGGGAAACCT CCAAGAAAAC CAGACAGAAG
    AGCAGCCCCC GGAAGCCCGA ACCTCTCAGC CCTGGGAAGT CAGCCAGGGA TGCCTCACCA
    AGAGACCTGA CAACCAAGCC TAGCCGGCCG TCCACCCCAG CCCTGGTCCT TGCCCCAGCT
    TACCTCCTGT CCTCCAGCCC CCAGCCCACC AGCAGCAGCT TCCCTTTCTT CCACCTGCTG
    GGCCCTACAC CTTTCCCGAT GCTGATGGGG CCTCCAGGCT CCAAGGGAGA CTGTGGCTTG
    CCGGGTCCCC CTGGGCTTCC AGGATTACCT GGATCTCCGG GCGCACGAGG TCCTCGGGGT
    CCTCCTGGGC CGTACGGGAA CCCAGGCCCA CCTGGCCCTC CAGGAGCCAA AGGACAGAAA
    GGGGACCCAG GACTCTCACC AGGACAGGCT CACGATGGAG CAAAGGGCAA CATGGGCTTG
    CCTGGGCTCT CCGGAAATCC AGGACCCTTG GGAAGGAAGG GACACAAGGG CCATCCTGGA
    GCAGCAGGGC ACCCTGGAGA ACAGGGGCAG CCAGGACCTG AGGGCAGTCC AGGGGCCAAA
    GGTTACCCTG GCAGGCAGGG GTTCCCTGGA CCTGTAGGAG ACCCTGGCCC CAAAGGCAGC
    AGGGGTTACA TTGGTCTTCC GGGGCTCTTT GGCCTGCCAG GGTCGGATGG AGAGCGGGGT
    CTCCCTGGCG TTCCTGGCAA GAGGGGCGAG ATGGGTAGGC CGGGCTTCCC TGGAGACTTT
    GGGGAAAGAG GCCCCCCTGG ACTTGATGGC AACCCTGGAG AAATTGGCCT GCCGGGGCCA
    CCAGGAGTGC TCGGGCTCAT TGGTGACACA GGAGCACTGG GCCCAGTTGG CTACCCAGGA
    CCGAAAGGCA TGAAGGGACT GATGGGCGGC GTGGGGGAGC CCGGACTGAA AGGTGATAAG
    GGTGAACAAG GGGTGCCAGG TGTGTCGGGA GATCCTGGCT TCCAAGGAGA CAAGGGGAGT
    CATGGCTTGC CAGGGCTCCC GGGTGGCCGG GGGAAGCCAG GGCCTCTGGG AAAAGCTGGA
    GACAAAGGAT CACTTGGGTT TCCTGGACCT CCTGGACCTG AGGGTTTCCC AGGAGACATT
    GGCCCTCCAG GGGACAATGG CCCTGAAGGC ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG
    CCTGGACCTC CTGGGCAGCT GGGGCCTGAG GGAGATGAAG GACCCATGGG GCCACCAGGA
    GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT
    GGCTCGAAGG GGGAACCAGG GGATCCTGGA CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT
    ATGGGATTCA TTGGTCTTGT CGGAGAGCCT GGAATTGTTG GGGAGAAGGG AGACCGTGGG
    GTGATGGGAC CCCCAGGTGC ACCTGGACCC AAGGGGTCGA TGGGCCATCC TGGAACACCC
    GGTGGTATTG GGAACCCTGG AGAGCCTGGA CCCTGGGGCC CTCCAGGATC TCGAGGCCTG
    CCAGGCATGA GGGGAGCCAA GGGGCACCGG GGCCCTCGAG GACCTGATGG ACCAGCTGGG
    GAGCAGGGAT CCAAGGGCCT GAAGGGTCGT GTAGGACCTC GGGGCAGACC TGGCCAGCCA
    GGACAGCAGG GTGCAGCTGG TGAGCGAGGC CACTCAGGTG CAAAAGGCTT CCTTGGCATC
    CCTGGTCCCT CAGGCCCTCC AGGTGCCAAG GGCCTCCCAG GAGAACCGGG CTCTCAGGGA
    CCTCAGGGGC CAGTTGGTCC TCCAGGAGAA ATGGGGCCCA AGGGACCGCC GGGTGCAGTG
    GGAGAGCCTG GCCTTCCTGG GGACTCCGGA ATGAAGGGTG ACCTCGGACC TCTGGGCCCC
    CCTGGGGAGC AAGGTCTTAT TGGGCAGCGG GGAGAGCCAG GCCTTGAGGG AGACCATGGC
    CCTGTGGGGC CAGATGGGCT GAAGGGCGAC AGGGGCGACC CAGGGCCCGA TGGCGAGCAT
    GGTGAAAAGG GCCAGGAAGG GCTGAAAGGA GAGGATGGGT CCCCTGGGCC CCCGGGCATC
    ACTGGCGTCC CGGGTCGTGA GGGGAAGCCG GGCAAGCAGG GTGAGAAAGG CCAGAGGGGA
    GCTAAGGGTG CCAAGGGCCA CCAAGGATAT CTGGGAGAGA TGGGCATCCC CGGAGAACCT
    GGGCCCCCTG GGACCCCAGG TCCTAAAGGG TCCCGGGGCA CCCTAGGACC AACGGGTGCT
    CCAGGACGGA TGGGAGCCCA AGGAGAACCA GGATTGGCCG GTTATAATGG ACACAAAGGC
    ATCACAGGAC CCCTCGGGCC TCCCGGGCCC AAAGGCGAGA AGGGGGACCA GGGCGAGGAT
    GGCAAGACCG AGGGGCCCCC TGGGCCACCA GGAGATCGGG GTCCTGTGGG TGATCGAGGA
    GACCGTGGGG AGCCAGGCGA CCCTGGATAC CCTGGTCAAG AGGGAGTTCA AGGCCTCCGT
    GGAGAACCGG GCCAGCAGGG ACAGCCTGGG CATCCAGGAC CCCGGGGGCG CCCAGGACCC
    AAAGGATCAA AAGGCGAAGA GGGCCCAAAG GGAAAGCCAG GCAAGGCTGG GCCATCAGGC
    CGGAGGGGGA CCCAGGGCCT TCAAGGACTG CCAGGCCCCC GAGGCGTGGT GGGGAGACAG
    GGCCCTGAGG GCACTGCTGG ATCTGATGGG ATTCCTGGCA GAGATGGTCG GCCAGGATAT
    CAGGGAGACC AGGGAAATGA TGGGGACCCT GGCCCTGTGG GCCCTGCTGG GAGAAGAGGA
    AATCCAGGTG TGGCTGGCTT GCCTGGAGCA CAGGGGCCTC CGGGATTCAA GGGTGAAAGT
    GGGTTACCTG GGCAACTGGG TCCCCCTGGA AAACGAGGGA CAGAAGGTGG AACAGGGCTT
    CCTGGGAACC AGGGGGAGCC AGGATCCAAA GGCCAGCCGG GTGACTCTGG CGAGATGGGC
    TTCCCAGGAG TGGCTGGCCT CTTTGGACCC AAGGGCCCCC CTGGAGACAT TGGCTTCAAA
    GGCATCCAAG GTCCTCGGGG GCCTCCTGGC TTGATGGGAA AGGAAGGTAT CATTGGCCCC
    CCCGGAATGC TGGGACCTTC TGGACTCCCG GGTCCCAAAG GTGACAGAGG CAGCCGAGGA
    GACTTGGGAC TGCAAGGCCC AAGGGGTCCT CCTGGTCCAA GGGGCCGGCC AGGTCCCCCG
    GGCCCTCCTT GGCATCCCAT CCAGTTTCAG CAAGATGACC TTGGAGCAGC TTTCCAGACA
    TGGATGGATG CTCAAGGAGC CGTCAGATCG GAGCAGGGGT ACAGCTATCC GGACCAGCTG
    GCGCTAGACC AGGGAGGGGA GATCTTCAAA ACCTTACACT ACCTCAGCAA CCTCATCCAG
    AGCATTAAGA CACCCTTGGG CACCAAGGAG AACCCAGCCC GGGTCTGCCG GGACCTCATG
    GACTGTGAGC AGAGGATGGC GGATGGTACC TACTGGGTGG ACCCCAACCT CGGCTGCTCC
    TCTGACACCA TTGAAGTCTC CTGCAACTTT ACACAGGGTG GGCAGACGTG CCTGAAGCCC
    ATCACGGCCT CCAAGGCCGA GTTTGCTGTG AGTCGGGTCC AGATGAATTT CTTGCACCTG
    CTGAGCTCTG AGGGGACACA GCATATCACA ATCCACTGTC TGAACATGAC GGTGTGGCAG
    GAGGGACCGG GACGCTCCTC TGCCAGACAG GCTGTGCGCT TCCGTGCCTG GAACGGACAG
    GTCTTCGAAG CTGGGGGTCA GTTCAGGCCA GAGGTGTCTA TGGATGGCTG CAAGGTCCAT
    GATGGCCGCT GGCATCAGAC ACTGTTCACC TTCCGGACCC AGGACCCCCA GCAGCTGCCC
    ATCGTCAGTG TGGACAATCT CCCGCCTGTC TCATCAGGGA AGCAGTACCG CCTGGAAGTT
    GGACCTGCGT GCTTCCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.