Col27a1 gene cDNA ORF clone, Mus musculus(house mouse)

mRNA	Protein	Name
XM_017320284.1	XP_017175773.1	collagen alpha-1(XXVII) chain isoform X3
XM_030253631.1	XP_030109491.1	collagen alpha-1(XXVII) chain isoform X4
XM_011250057.3	XP_011248359.1	collagen alpha-1(XXVII) chain isoform X5
NM_025685.3	NP_079961.3	collagen alpha-1(XXVII) chain preproprotein
XM_017320285.1	XP_017175774.1	collagen alpha-1(XXVII) chain isoform X6
XM_006538035.4	XP_006538098.1	collagen alpha-1(XXVII) chain isoform X1
XM_030253632.1	XP_030109492.1	collagen alpha-1(XXVII) chain isoform X6
XM_011250058.3	XP_011248360.1	collagen alpha-1(XXVII) chain isoform X8
XM_017320283.1	XP_017175772.1	collagen alpha-1(XXVII) chain isoform X2
XM_006538035.3	XP_006538098.1	collagen alpha-1(XXVII) chain isoform X4
XM_030253629.1	XP_030109489.1	collagen alpha-1(XXVII) chain isoform X2
XM_030253630.1	XP_030109490.1	collagen alpha-1(XXVII) chain isoform X3
XM_030253633.1	XP_030109493.1	collagen alpha-1(XXVII) chain isoform X7
XM_017320282.1	XP_017175771.1	collagen alpha-1(XXVII) chain isoform X1
XM_011250058.2	XP_011248360.1	collagen alpha-1(XXVII) chain isoform X7
XM_006538039.3	XP_006538102.1	collagen alpha-1(XXVII) chain isoform X8
XM_030253634.1	XP_030109494.1	collagen alpha-1(XXVII) chain isoform X10
XM_011250057.2	XP_011248359.1	collagen alpha-1(XXVII) chain isoform X5
XM_006538040.3	XP_006538103.1	collagen alpha-1(XXVII) chain isoform X9
XM_017320286.1	XP_017175775.1	collagen alpha-1(XXVII) chain isoform X10
XM_006538039.4	XP_006538102.1	collagen alpha-1(XXVII) chain isoform X9

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu81040	XM_017320286.1 Latest version!	Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu88569	XM_030253634.1 Latest version!	Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu33009	XM_006538039.4 Latest version!	Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu74976	XM_011250057.3 Latest version!	Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu74977	XM_011250058.3 Latest version!	Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu81039	XM_017320285.1 Latest version!	Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu88567	XM_030253632.1 Latest version!	Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu88568	XM_030253633.1 Latest version!	Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu33010	XM_006538040.3 Latest version!	Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu33005	XM_006538035.4 Latest version!	Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu81036	XM_017320282.1 Latest version!	Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu81037	XM_017320283.1 Latest version!	Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu81038	XM_017320284.1 Latest version!	Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu88564	XM_030253629.1 Latest version!	Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu88565	XM_030253630.1 Latest version!	Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu88566	XM_030253631.1 Latest version!	Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu20706	NM_025685.3 Latest version!	Mus musculus collagen, type XXVII, alpha 1 (Col27a1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
View Old Accession Versions >>
OMu33005	XM_006538035.3	Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu74977	XM_011250058.2	Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu74976	XM_011250057.2	Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu33009	XM_006538039.3	Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
Hide Old Accession Versions >>

Col27a1 ( XM_017320286.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175775.1 Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OMu81040
Clone ID Related Accession (Same CDS sequence)	XM_017320286.1
Accession Version	XM_017320286.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2886bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	collagen alpha-1(XXVII) chain isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG CCTGGACCTC CTGGGCAGCT GGGGCCTGAG 
GGAGATGAAG GACCCATGGG GCCACCAGGA GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG 
AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT GGCTCGAAGG GGGAACCAGG GGATCCTGGA 
CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT ATGGGATTCA TTGGTCTTGT CGGAGAGCCT 
GGAATTGTTG GGGAGAAGGG AGACCGTGGG GTGATGGGAC CCCCAGGTGC ACCTGGACCC 
AAGGGGTCGA TGGGCCATCC TGGAACACCC GGTGGTATTG GGAACCCTGG AGAGCCTGGA 
CCCTGGGGCC CTCCAGGATC TCGAGGCCTG CCAGGCATGA GGGGAGCCAA GGGGCACCGG 
GGCCCTCGAG GACCTGATGG ACCAGCTGGG GAGCAGGGAT CCAAGGGCCT GAAGGGTCGT 
GTAGGACCTC GGGGCAGACC TGGCCAGCCA GGACAGCAGG GTGCAGCTGG TGAGCGAGGC 
CACTCAGGTG CAAAAGGCTT CCTTGGCATC CCTGGTCCCT CAGGCCCTCC AGGTGCCAAG 
GGCCTCCCAG GAGAACCGGG CTCTCAGGGA CCTCAGGGGC CAGTTGGTCC TCCAGGAGAA 
ATGGGGCCCA AGGGACCGCC GGGTGCAGTG GGAGAGCCTG GCCTTCCTGG GGACTCCGGA 
ATGAAGGGTG ACCTCGGACC TCTGGGCCCC CCTGGGGAGC AAGGTCTTAT TGGGCAGCGG 
GGAGAGCCAG GCCTTGAGGG AGACCATGGC CCTGTGGGGC CAGATGGGCT GAAGGGCGAC 
AGGGGCGACC CAGGGCCCGA TGGCGAGCAT GGTGAAAAGG GCCAGGAAGG GCTGAAAGGA 
GAGGATGGGT CCCCTGGGCC CCCGGGCATC ACTGGCGTCC CGGGTCGTGA GGGGAAGCCG 
GGCAAGCAGG GTGAGAAAGG CCAGAGGGGA GCTAAGGGTG CCAAGGGCCA CCAAGGATAT 
CTGGGAGAGA TGGGCATCCC CGGAGAACCT GGGCCCCCTG GGACCCCAGG TCCTAAAGGG 
TCCCGGGGCA CCCTAGGACC AACGGGTGCT CCAGGACGGA TGGGAGCCCA AGGAGAACCA 
GGATTGGCCG GTTATAATGG ACACAAAGGC ATCACAGGAC CCCTCGGGCC TCCCGGGCCC 
AAAGGCGAGA AGGGGGACCA GGGCGAGGAT GGCAAGACCG AGGGGCCCCC TGGGCCACCA 
GGAGATCGGG GTCCTGTGGG TGATCGAGGA GACCGTGGGG AGCCAGGCGA CCCTGGATAC 
CCTGGTCAAG AGGGAGTTCA AGGCCTCCGT GGAGAACCGG GCCAGCAGGG ACAGCCTGGG 
CATCCAGGAC CCCGGGGGCG CCCAGGACCC AAAGGATCAA AAGGCGAAGA GGGCCCAAAG 
GGAAAGCCAG GCAAGGCTGG GCCATCAGGC CGGAGGGGGA CCCAGGTGAG TTGCCATAGC 
TTCTCTGTCA CCTTCTATGT TCTCCTGAGA AGGTTTAACC AGCTTCTTCT GTACCTCCAG 
GGCCTTCAAG GACTGCCAGG CCCCCGAGGC GTGGTGGGGA GACAGGGCCC TGAGGGCACT 
GCTGGATCTG ATGGGATTCC TGGCAGAGAT GGTCGGCCAG GATATCAGGG AGACCAGGGA 
AATGATGGGG ACCCTGGCCC TGTGGGCCCT GCTGGGAGAA GAGGAAATCC AGGTGTGGCT 
GGCTTGCCTG GAGCACAGGG GCCTCCGGGA TTCAAGGGTG AAAGTGGGTT ACCTGGGCAA 
CTGGGTCCCC CTGGAAAACG AGGGACAGAA GGTGGAACAG GGCTTCCTGG GAACCAGGGG 
GAGCCAGGAT CCAAAGGCCA GCCGGGTGAC TCTGGCGAGA TGGGCTTCCC AGGAGTGGCT 
GGCCTCTTTG GACCCAAGGG CCCCCCTGGA GACATTGGCT TCAAAGGCAT CCAAGGTCCT 
CGGGGGCCTC CTGGCTTGAT GGGAAAGGAA GGTATCATTG GCCCCCCCGG AATGCTGGGA 
CCTTCTGGAC TCCCGGGTCC CAAAGGTGAC AGAGGCAGCC GAGGAGACTT GGGACTGCAA 
GGCCCAAGGG GTCCTCCTGG TCCAAGGGGC CGGCCAGGTC CCCCGGGCCC TCCTTGGCAT 
CCCATCCAGT TTCAGCAAGA TGACCTTGGA GCAGCTTTCC AGACATGGAT GGATGCTCAA 
GGAGCCGTCA GATCGGAGCA GGGGTACAGC TATCCGGACC AGCTGGCGCT AGACCAGGGA 
GGGGAGATCT TCAAAACCTT ACACTACCTC AGCAACCTCA TCCAGAGCAT TAAGACACCC 
TTGGGCACCA AGGAGAACCC AGCCCGGGTC TGCCGGGACC TCATGGACTG TGAGCAGAGG 
ATGGCGGATG GTACCTACTG GGTGGACCCC AACCTCGGCT GCTCCTCTGA CACCATTGAA 
GTCTCCTGCA ACTTTACACA GGGTGGGCAG ACGTGCCTGA AGCCCATCAC GGCCTCCAAG 
GCCGAGTTTG CTGTGAGTCG GGTCCAGATG AATTTCTTGC ACCTGCTGAG CTCTGAGGGG 
ACACAGCATA TCACAATCCA CTGTCTGAAC ATGACGGTGT GGCAGGAGGG ACCGGGACGC 
TCCTCTGCCA GACAGGCTGT GCGCTTCCGT GCCTGGAACG GACAGGTCTT CGAAGCTGGG 
GGTCAGTTCA GGCCAGAGGT GTCTATGGAT GGCTGCAAGG TCCATGATGG CCGCTGGCAT 
CAGACACTGT TCACCTTCCG GACCCAGGAC CCCCAGCAGC TGCCCATCGT CAGTGTGGAC 
AATCTCCCGC CTGTCTCATC AGGGAAGCAG TACCGCCTGG AAGTTGGACC TGCGTGCTTC 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175775.1
CDS	1532..4417
Translation	MKGKPGARGLPGPPGQLGPEGDEGPMGPPGVPGLEGQPGRKGFPGRPGLDGSKGEPGDPGRPGPVGEQGLMGFIGLVGEPGIVGEKGDRGVMGPPGAPGPKGSMGHPGTPGGIGNPGEPGPWGPPGSRGLPGMRGAKGHRGPRGPDGPAGEQGSKGLKGRVGPRGRPGQPGQQGAAGERGHSGAKGFLGIPGPSGPPGAKGLPGEPGSQGPQGPVGPPGEMGPKGPPGAVGEPGLPGDSGMKGDLGPLGPPGEQGLIGQRGEPGLEGDHGPVGPDGLKGDRGDPGPDGEHGEKGQEGLKGEDGSPGPPGITGVPGREGKPGKQGEKGQRGAKGAKGHQGYLGEMGIPGEPGPPGTPGPKGSRGTLGPTGAPGRMGAQGEPGLAGYNGHKGITGPLGPPGPKGEKGDQGEDGKTEGPPGPPGDRGPVGDRGDRGEPGDPGYPGQEGVQGLRGEPGQQGQPGHPGPRGRPGPKGSKGEEGPKGKPGKAGPSGRRGTQVSCHSFSVTFYVLLRRFNQLLLYLQGLQGLPGPRGVVGRQGPEGTAGSDGIPGRDGRPGYQGDQGNDGDPGPVGPAGRRGNPGVAGLPGAQGPPGFKGESGLPGQLGPPGKRGTEGGTGLPGNQGEPGSKGQPGDSGEMGFPGVAGLFGPKGPPGDIGFKGIQGPRGPPGLMGKEGIIGPPGMLGPSGLPGPKGDRGSRGDLGLQGPRGPPGPRGRPGPPGPPWHPIQFQQDDLGAAFQTWMDAQGAVRSEQGYSYPDQLALDQGGEIFKTLHYLSNLIQSIKTPLGTKENPARVCRDLMDCEQRMADGTYWVDPNLGCSSDTIEVSCNFTQGGQTCLKPITASKAEFAVSRVQMNFLHLLSSEGTQHITIHCLNMTVWQEGPGRSSARQAVRFRAWNGQVFEAGGQFRPEVSMDGCKVHDGRWHQTLFTFRTQDPQQLPIVSVDNLPPVSSGKQYRLEVGPACFL

Target ORF information:

RefSeq Version	XM_017320286.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320286.1

ORF Insert Sequence:

ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG CCTGGACCTC CTGGGCAGCT GGGGCCTGAG 
GGAGATGAAG GACCCATGGG GCCACCAGGA GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG 
AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT GGCTCGAAGG GGGAACCAGG GGATCCTGGA 
CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT ATGGGATTCA TTGGTCTTGT CGGAGAGCCT 
GGAATTGTTG GGGAGAAGGG AGACCGTGGG GTGATGGGAC CCCCAGGTGC ACCTGGACCC 
AAGGGGTCGA TGGGCCATCC TGGAACACCC GGTGGTATTG GGAACCCTGG AGAGCCTGGA 
CCCTGGGGCC CTCCAGGATC TCGAGGCCTG CCAGGCATGA GGGGAGCCAA GGGGCACCGG 
GGCCCTCGAG GACCTGATGG ACCAGCTGGG GAGCAGGGAT CCAAGGGCCT GAAGGGTCGT 
GTAGGACCTC GGGGCAGACC TGGCCAGCCA GGACAGCAGG GTGCAGCTGG TGAGCGAGGC 
CACTCAGGTG CAAAAGGCTT CCTTGGCATC CCTGGTCCCT CAGGCCCTCC AGGTGCCAAG 
GGCCTCCCAG GAGAACCGGG CTCTCAGGGA CCTCAGGGGC CAGTTGGTCC TCCAGGAGAA 
ATGGGGCCCA AGGGACCGCC GGGTGCAGTG GGAGAGCCTG GCCTTCCTGG GGACTCCGGA 
ATGAAGGGTG ACCTCGGACC TCTGGGCCCC CCTGGGGAGC AAGGTCTTAT TGGGCAGCGG 
GGAGAGCCAG GCCTTGAGGG AGACCATGGC CCTGTGGGGC CAGATGGGCT GAAGGGCGAC 
AGGGGCGACC CAGGGCCCGA TGGCGAGCAT GGTGAAAAGG GCCAGGAAGG GCTGAAAGGA 
GAGGATGGGT CCCCTGGGCC CCCGGGCATC ACTGGCGTCC CGGGTCGTGA GGGGAAGCCG 
GGCAAGCAGG GTGAGAAAGG CCAGAGGGGA GCTAAGGGTG CCAAGGGCCA CCAAGGATAT 
CTGGGAGAGA TGGGCATCCC CGGAGAACCT GGGCCCCCTG GGACCCCAGG TCCTAAAGGG 
TCCCGGGGCA CCCTAGGACC AACGGGTGCT CCAGGACGGA TGGGAGCCCA AGGAGAACCA 
GGATTGGCCG GTTATAATGG ACACAAAGGC ATCACAGGAC CCCTCGGGCC TCCCGGGCCC 
AAAGGCGAGA AGGGGGACCA GGGCGAGGAT GGCAAGACCG AGGGGCCCCC TGGGCCACCA 
GGAGATCGGG GTCCTGTGGG TGATCGAGGA GACCGTGGGG AGCCAGGCGA CCCTGGATAC 
CCTGGTCAAG AGGGAGTTCA AGGCCTCCGT GGAGAACCGG GCCAGCAGGG ACAGCCTGGG 
CATCCAGGAC CCCGGGGGCG CCCAGGACCC AAAGGATCAA AAGGCGAAGA GGGCCCAAAG 
GGAAAGCCAG GCAAGGCTGG GCCATCAGGC CGGAGGGGGA CCCAGGTGAG TTGCCATAGC 
TTCTCTGTCA CCTTCTATGT TCTCCTGAGA AGGTTTAACC AGCTTCTTCT GTACCTCCAG 
GGCCTTCAAG GACTGCCAGG CCCCCGAGGC GTGGTGGGGA GACAGGGCCC TGAGGGCACT 
GCTGGATCTG ATGGGATTCC TGGCAGAGAT GGTCGGCCAG GATATCAGGG AGACCAGGGA 
AATGATGGGG ACCCTGGCCC TGTGGGCCCT GCTGGGAGAA GAGGAAATCC AGGTGTGGCT 
GGCTTGCCTG GAGCACAGGG GCCTCCGGGA TTCAAGGGTG AAAGTGGGTT ACCTGGGCAA 
CTGGGTCCCC CTGGAAAACG AGGGACAGAA GGTGGAACAG GGCTTCCTGG GAACCAGGGG 
GAGCCAGGAT CCAAAGGCCA GCCGGGTGAC TCTGGCGAGA TGGGCTTCCC AGGAGTGGCT 
GGCCTCTTTG GACCCAAGGG CCCCCCTGGA GACATTGGCT TCAAAGGCAT CCAAGGTCCT 
CGGGGGCCTC CTGGCTTGAT GGGAAAGGAA GGTATCATTG GCCCCCCCGG AATGCTGGGA 
CCTTCTGGAC TCCCGGGTCC CAAAGGTGAC AGAGGCAGCC GAGGAGACTT GGGACTGCAA 
GGCCCAAGGG GTCCTCCTGG TCCAAGGGGC CGGCCAGGTC CCCCGGGCCC TCCTTGGCAT 
CCCATCCAGT TTCAGCAAGA TGACCTTGGA GCAGCTTTCC AGACATGGAT GGATGCTCAA 
GGAGCCGTCA GATCGGAGCA GGGGTACAGC TATCCGGACC AGCTGGCGCT AGACCAGGGA 
GGGGAGATCT TCAAAACCTT ACACTACCTC AGCAACCTCA TCCAGAGCAT TAAGACACCC 
TTGGGCACCA AGGAGAACCC AGCCCGGGTC TGCCGGGACC TCATGGACTG TGAGCAGAGG 
ATGGCGGATG GTACCTACTG GGTGGACCCC AACCTCGGCT GCTCCTCTGA CACCATTGAA 
GTCTCCTGCA ACTTTACACA GGGTGGGCAG ACGTGCCTGA AGCCCATCAC GGCCTCCAAG 
GCCGAGTTTG CTGTGAGTCG GGTCCAGATG AATTTCTTGC ACCTGCTGAG CTCTGAGGGG 
ACACAGCATA TCACAATCCA CTGTCTGAAC ATGACGGTGT GGCAGGAGGG ACCGGGACGC 
TCCTCTGCCA GACAGGCTGT GCGCTTCCGT GCCTGGAACG GACAGGTCTT CGAAGCTGGG 
GGTCAGTTCA GGCCAGAGGT GTCTATGGAT GGCTGCAAGG TCCATGATGG CCGCTGGCAT 
CAGACACTGT TCACCTTCCG GACCCAGGAC CCCCAGCAGC TGCCCATCGT CAGTGTGGAC 
AATCTCCCGC CTGTCTCATC AGGGAAGCAG TACCGCCTGG AAGTTGGACC TGCGTGCTTC 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col27a1 ( XM_030253634.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030109494.1 Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OMu88569
Clone ID Related Accession (Same CDS sequence)	XM_030253634.1
Accession Version	XM_030253634.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2811bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	collagen alpha-1(XXVII) chain isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG CCTGGACCTC CTGGGCAGCT GGGGCCTGAG 
GGAGATGAAG GACCCATGGG GCCACCAGGA GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG 
AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT GGCTCGAAGG GGGAACCAGG GGATCCTGGA 
CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT ATGGGATTCA TTGGTCTTGT CGGAGAGCCT 
GGAATTGTTG GGGAGAAGGG AGACCGTGGG GTGATGGGAC CCCCAGGTGC ACCTGGACCC 
AAGGGGTCGA TGGGCCATCC TGGAACACCC GGTGGTATTG GGAACCCTGG AGAGCCTGGA 
CCCTGGGGCC CTCCAGGATC TCGAGGCCTG CCAGGCATGA GGGGAGCCAA GGGGCACCGG 
GGCCCTCGAG GACCTGATGG ACCAGCTGGG GAGCAGGGAT CCAAGGGCCT GAAGGGTCGT 
GTAGGACCTC GGGGCAGACC TGGCCAGCCA GGACAGCAGG GTGCAGCTGG TGAGCGAGGC 
CACTCAGGTG CAAAAGGCTT CCTTGGCATC CCTGGTCCCT CAGGCCCTCC AGGTGCCAAG 
GGCCTCCCAG GAGAACCGGG CTCTCAGGGA CCTCAGGGGC CAGTTGGTCC TCCAGGAGAA 
ATGGGGCCCA AGGGACCGCC GGGTGCAGTG GGAGAGCCTG GCCTTCCTGG GGACTCCGGA 
ATGAAGGGTG ACCTCGGACC TCTGGGCCCC CCTGGGGAGC AAGGTCTTAT TGGGCAGCGG 
GGAGAGCCAG GCCTTGAGGG AGACCATGGC CCTGTGGGGC CAGATGGGCT GAAGGGCGAC 
AGGGGCGACC CAGGGCCCGA TGGCGAGCAT GGTGAAAAGG GCCAGGAAGG GCTGAAAGGA 
GAGGATGGGT CCCCTGGGCC CCCGGGCATC ACTGGCGTCC CGGGTCGTGA GGGGAAGCCG 
GGCAAGCAGG GTGAGAAAGG CCAGAGGGGA GCTAAGGGTG CCAAGGGCCA CCAAGGATAT 
CTGGGAGAGA TGGGCATCCC CGGAGAACCT GGGCCCCCTG GGACCCCAGG TCCTAAAGGG 
TCCCGGGGCA CCCTAGGACC AACGGGTGCT CCAGGACGGA TGGGAGCCCA AGGAGAACCA 
GGATTGGCCG GTTATAATGG ACACAAAGGC ATCACAGGAC CCCTCGGGCC TCCCGGGCCC 
AAAGGCGAGA AGGGGGACCA GGGCGAGGAT GGCAAGACCG AGGGGCCCCC TGGGCCACCA 
GGAGATCGGG GTCCTGTGGG TGATCGAGGA GACCGTGGGG AGCCAGGCGA CCCTGGATAC 
CCTGGTCAAG AGGGAGTTCA AGGCCTCCGT GGAGAACCGG GCCAGCAGGG ACAGCCTGGG 
CATCCAGGAC CCCGGGGGCG CCCAGGACCC AAAGGATCAA AAGGCGAAGA GGGCCCAAAG 
GGAAAGCCAG GCAAGGCTGG GCCATCAGGC CGGAGGGGGA CCCAGGGCCT TCAAGGACTG 
CCAGGCCCCC GAGGCGTGGT GGGGAGACAG GGCCCTGAGG GCACTGCTGG ATCTGATGGG 
ATTCCTGGCA GAGATGGTCG GCCAGGATAT CAGGGAGACC AGGGAAATGA TGGGGACCCT 
GGCCCTGTGG GCCCTGCTGG GAGAAGAGGA AATCCAGGTG TGGCTGGCTT GCCTGGAGCA 
CAGGGGCCTC CGGGATTCAA GGGTGAAAGT GGGTTACCTG GGCAACTGGG TCCCCCTGGA 
AAACGAGGGA CAGAAGGTGG AACAGGGCTT CCTGGGAACC AGGGGGAGCC AGGATCCAAA 
GGCCAGCCGG GTGACTCTGG CGAGATGGGC TTCCCAGGAG TGGCTGGCCT CTTTGGACCC 
AAGGGCCCCC CTGGAGACAT TGGCTTCAAA GGCATCCAAG GTCCTCGGGG GCCTCCTGGC 
TTGATGGGAA AGGAAGGTAT CATTGGCCCC CCCGGAATGC TGGGACCTTC TGGACTCCCG 
GGTCCCAAAG GTGACAGAGG CAGCCGAGGA GACTTGGGAC TGCAAGGCCC AAGGGGTCCT 
CCTGGTCCAA GGGGCCGGCC AGGTCCCCCG GGCCCTCCTT GGCATCCCAT CCAGTTTCAG 
CAAGATGACC TTGGAGCAGC TTTCCAGACA TGGATGGATG CTCAAGGAGC CGTCAGATCG 
GAGCAGGGGT ACAGCTATCC GGACCAGCTG GCGCTAGACC AGGGAGGGGA GATCTTCAAA 
ACCTTACACT ACCTCAGCAA CCTCATCCAG AGCATTAAGA CACCCTTGGG CACCAAGGAG 
AACCCAGCCC GGGTCTGCCG GGACCTCATG GACTGTGAGC AGAGGATGGC GGATGGTACC 
TACTGGGTGG ACCCCAACCT CGGCTGCTCC TCTGACACCA TTGAAGTCTC CTGCAACTTT 
ACACAGGGTG GGCAGACGTG CCTGAAGCCC ATCACGGCCT CCAAGGCCGA GTTTGCTGTG 
AGTCGGGTCC AGATGAATTT CTTGCACCTG CTGAGCTCTG AGGGGACACA GCATATCACA 
ATCCACTGTC TGAACATGAC GGTGTGGCAG GAGGGACCGG GACGCTCCTC TGCCAGACAG 
GCTGTGCGCT TCCGTGCCTG GAACGGACAG GTCTTCGAAG CTGGGGGTCA GTTCAGGCCA 
GAGGTGTCTA TGGATGGCTG CAAGGTCCAT GATGGCCGCT GGCATCAGAC ACTGTTCACC 
TTCCGGACCC AGGACCCCCA GCAGCTGCCC ATCGTCAGTG TGGACAATCT CCCGCCTGTC 
TCATCAGGGA AGCAGTACCG CCTGGAAGTT GGACCTGCGT GCTTCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030109494.1
CDS	545..3355
Translation	MKGKPGARGLPGPPGQLGPEGDEGPMGPPGVPGLEGQPGRKGFPGRPGLDGSKGEPGDPGRPGPVGEQGLMGFIGLVGEPGIVGEKGDRGVMGPPGAPGPKGSMGHPGTPGGIGNPGEPGPWGPPGSRGLPGMRGAKGHRGPRGPDGPAGEQGSKGLKGRVGPRGRPGQPGQQGAAGERGHSGAKGFLGIPGPSGPPGAKGLPGEPGSQGPQGPVGPPGEMGPKGPPGAVGEPGLPGDSGMKGDLGPLGPPGEQGLIGQRGEPGLEGDHGPVGPDGLKGDRGDPGPDGEHGEKGQEGLKGEDGSPGPPGITGVPGREGKPGKQGEKGQRGAKGAKGHQGYLGEMGIPGEPGPPGTPGPKGSRGTLGPTGAPGRMGAQGEPGLAGYNGHKGITGPLGPPGPKGEKGDQGEDGKTEGPPGPPGDRGPVGDRGDRGEPGDPGYPGQEGVQGLRGEPGQQGQPGHPGPRGRPGPKGSKGEEGPKGKPGKAGPSGRRGTQGLQGLPGPRGVVGRQGPEGTAGSDGIPGRDGRPGYQGDQGNDGDPGPVGPAGRRGNPGVAGLPGAQGPPGFKGESGLPGQLGPPGKRGTEGGTGLPGNQGEPGSKGQPGDSGEMGFPGVAGLFGPKGPPGDIGFKGIQGPRGPPGLMGKEGIIGPPGMLGPSGLPGPKGDRGSRGDLGLQGPRGPPGPRGRPGPPGPPWHPIQFQQDDLGAAFQTWMDAQGAVRSEQGYSYPDQLALDQGGEIFKTLHYLSNLIQSIKTPLGTKENPARVCRDLMDCEQRMADGTYWVDPNLGCSSDTIEVSCNFTQGGQTCLKPITASKAEFAVSRVQMNFLHLLSSEGTQHITIHCLNMTVWQEGPGRSSARQAVRFRAWNGQVFEAGGQFRPEVSMDGCKVHDGRWHQTLFTFRTQDPQQLPIVSVDNLPPVSSGKQYRLEVGPACFL

Target ORF information:

RefSeq Version	XM_030253634.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030253634.1

ORF Insert Sequence:

ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG CCTGGACCTC CTGGGCAGCT GGGGCCTGAG 
GGAGATGAAG GACCCATGGG GCCACCAGGA GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG 
AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT GGCTCGAAGG GGGAACCAGG GGATCCTGGA 
CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT ATGGGATTCA TTGGTCTTGT CGGAGAGCCT 
GGAATTGTTG GGGAGAAGGG AGACCGTGGG GTGATGGGAC CCCCAGGTGC ACCTGGACCC 
AAGGGGTCGA TGGGCCATCC TGGAACACCC GGTGGTATTG GGAACCCTGG AGAGCCTGGA 
CCCTGGGGCC CTCCAGGATC TCGAGGCCTG CCAGGCATGA GGGGAGCCAA GGGGCACCGG 
GGCCCTCGAG GACCTGATGG ACCAGCTGGG GAGCAGGGAT CCAAGGGCCT GAAGGGTCGT 
GTAGGACCTC GGGGCAGACC TGGCCAGCCA GGACAGCAGG GTGCAGCTGG TGAGCGAGGC 
CACTCAGGTG CAAAAGGCTT CCTTGGCATC CCTGGTCCCT CAGGCCCTCC AGGTGCCAAG 
GGCCTCCCAG GAGAACCGGG CTCTCAGGGA CCTCAGGGGC CAGTTGGTCC TCCAGGAGAA 
ATGGGGCCCA AGGGACCGCC GGGTGCAGTG GGAGAGCCTG GCCTTCCTGG GGACTCCGGA 
ATGAAGGGTG ACCTCGGACC TCTGGGCCCC CCTGGGGAGC AAGGTCTTAT TGGGCAGCGG 
GGAGAGCCAG GCCTTGAGGG AGACCATGGC CCTGTGGGGC CAGATGGGCT GAAGGGCGAC 
AGGGGCGACC CAGGGCCCGA TGGCGAGCAT GGTGAAAAGG GCCAGGAAGG GCTGAAAGGA 
GAGGATGGGT CCCCTGGGCC CCCGGGCATC ACTGGCGTCC CGGGTCGTGA GGGGAAGCCG 
GGCAAGCAGG GTGAGAAAGG CCAGAGGGGA GCTAAGGGTG CCAAGGGCCA CCAAGGATAT 
CTGGGAGAGA TGGGCATCCC CGGAGAACCT GGGCCCCCTG GGACCCCAGG TCCTAAAGGG 
TCCCGGGGCA CCCTAGGACC AACGGGTGCT CCAGGACGGA TGGGAGCCCA AGGAGAACCA 
GGATTGGCCG GTTATAATGG ACACAAAGGC ATCACAGGAC CCCTCGGGCC TCCCGGGCCC 
AAAGGCGAGA AGGGGGACCA GGGCGAGGAT GGCAAGACCG AGGGGCCCCC TGGGCCACCA 
GGAGATCGGG GTCCTGTGGG TGATCGAGGA GACCGTGGGG AGCCAGGCGA CCCTGGATAC 
CCTGGTCAAG AGGGAGTTCA AGGCCTCCGT GGAGAACCGG GCCAGCAGGG ACAGCCTGGG 
CATCCAGGAC CCCGGGGGCG CCCAGGACCC AAAGGATCAA AAGGCGAAGA GGGCCCAAAG 
GGAAAGCCAG GCAAGGCTGG GCCATCAGGC CGGAGGGGGA CCCAGGGCCT TCAAGGACTG 
CCAGGCCCCC GAGGCGTGGT GGGGAGACAG GGCCCTGAGG GCACTGCTGG ATCTGATGGG 
ATTCCTGGCA GAGATGGTCG GCCAGGATAT CAGGGAGACC AGGGAAATGA TGGGGACCCT 
GGCCCTGTGG GCCCTGCTGG GAGAAGAGGA AATCCAGGTG TGGCTGGCTT GCCTGGAGCA 
CAGGGGCCTC CGGGATTCAA GGGTGAAAGT GGGTTACCTG GGCAACTGGG TCCCCCTGGA 
AAACGAGGGA CAGAAGGTGG AACAGGGCTT CCTGGGAACC AGGGGGAGCC AGGATCCAAA 
GGCCAGCCGG GTGACTCTGG CGAGATGGGC TTCCCAGGAG TGGCTGGCCT CTTTGGACCC 
AAGGGCCCCC CTGGAGACAT TGGCTTCAAA GGCATCCAAG GTCCTCGGGG GCCTCCTGGC 
TTGATGGGAA AGGAAGGTAT CATTGGCCCC CCCGGAATGC TGGGACCTTC TGGACTCCCG 
GGTCCCAAAG GTGACAGAGG CAGCCGAGGA GACTTGGGAC TGCAAGGCCC AAGGGGTCCT 
CCTGGTCCAA GGGGCCGGCC AGGTCCCCCG GGCCCTCCTT GGCATCCCAT CCAGTTTCAG 
CAAGATGACC TTGGAGCAGC TTTCCAGACA TGGATGGATG CTCAAGGAGC CGTCAGATCG 
GAGCAGGGGT ACAGCTATCC GGACCAGCTG GCGCTAGACC AGGGAGGGGA GATCTTCAAA 
ACCTTACACT ACCTCAGCAA CCTCATCCAG AGCATTAAGA CACCCTTGGG CACCAAGGAG 
AACCCAGCCC GGGTCTGCCG GGACCTCATG GACTGTGAGC AGAGGATGGC GGATGGTACC 
TACTGGGTGG ACCCCAACCT CGGCTGCTCC TCTGACACCA TTGAAGTCTC CTGCAACTTT 
ACACAGGGTG GGCAGACGTG CCTGAAGCCC ATCACGGCCT CCAAGGCCGA GTTTGCTGTG 
AGTCGGGTCC AGATGAATTT CTTGCACCTG CTGAGCTCTG AGGGGACACA GCATATCACA 
ATCCACTGTC TGAACATGAC GGTGTGGCAG GAGGGACCGG GACGCTCCTC TGCCAGACAG 
GCTGTGCGCT TCCGTGCCTG GAACGGACAG GTCTTCGAAG CTGGGGGTCA GTTCAGGCCA 
GAGGTGTCTA TGGATGGCTG CAAGGTCCAT GATGGCCGCT GGCATCAGAC ACTGTTCACC 
TTCCGGACCC AGGACCCCCA GCAGCTGCCC ATCGTCAGTG TGGACAATCT CCCGCCTGTC 
TCATCAGGGA AGCAGTACCG CCTGGAAGTT GGACCTGCGT GCTTCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col27a1 ( XM_006538039.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006538102.1 Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X10, mRNA.

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CloneID	OMu33009
Clone ID Related Accession (Same CDS sequence)	XM_006538039.3 , XM_006538039.4
Accession Version	XM_006538039.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3186bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	collagen alpha-1(XXVII) chain isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006538039.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCACGG GATTCGCGCG GGGGGCCCGA GGCACAGCGG CGTCAGGACC CGGGGGGGGG 
TTTCTCTTCG CCTGGATCTT GGTCTCATTT ACCTGTCACC TGGCCTCCAC CCAAGGAGCT 
CCTGAAGATG TGGATGTCCT CCAGCGGCTG GGCCTCAGCT GGACGAAGGC CGGGGGTGGC 
CGGAGTCCTA CACCCCCTGG TGTCATTCCT TTCCCATCTG GCTTCATCTT CACACAGCGG 
GCCAAGCTGC AGGCCCCCAC AGCCAATGTT CTTCCCACCA CCCTGGGGCG GGAGCTGGCA 
TTGGTGTTGA GCCTCTGTTC ACATCGTGTG AACCATGCCT TCCTCTTTGC CATCCGCAGC 
AGGAAACACA AGCTGCAACT GGGTCTGCAG TTCCTCCCCG GCAGGACGAT CATCCACCTG 
GGGCCTCGGC AATCAGTGGC CTTCGACTTG GATGTGCATG ATGGGCGCTG GCACCACCTG 
GCCCTAGAGT TGCGTGGCCG CACAGTCACA ATGGTGACAG CCTGTGGGCA GCACAGGGTA 
CCTGTCCCAC TGCCTTCCCG TAGGGACTCC ATGCTTGACC CCCAGGGCTC CTTTCTCTTG 
GGGAAGGTGA ACCCCCGTGC TGTCCAGTTT GAAGGTGCAC TCTGTCAGTT CAGCATCCAC 
CCTGTGGCGC AGGTCGCTCA CAATTACTGT GCCCACCTGA GAGAGCGGTG CCGACAGGTG 
GACACATATA GTCCCCAGGT GGGAACCCTC TTTCCTTGGG ACTCTGGCCC AGCCTTTGCT 
TTGCATCCTG AACCAGCCTT GCTTGGCTTG GGGAATCTGA CCAGAACTCC AGCAACCCTG 
GGGGCCAGGC CTGTAAGCAG GGCACTTGCA GTGACTCTGG CTCCTGCCAT GCCCACCAAG 
CCCCTGAGGA CTGTCCACCC AGATGTCTCT GAACACAGCT CTTCCCAGAC CCCACTGTCA 
CCTGCCAAAC AGTCGGCCAG GAAAACACCT TCCCCCTCCT CGTCAGCTTC CCTGGCTAAT 
TCCACCAGGG TTTATCGTCC AGCTGCTGCC CAGCCAAGAC AGATCACAAC TACTAGCCCC 
ACCAAACGTT CCCCCACCAA GCCTTCTGTT TCTCCCCTTT CAGTTACCCC CATGAAAAGT 
CCCCATGCAA CCCAGAAAAC AGGTGTCCCT TCATTCACAA AGCCTGTTCC ACCCACTCAG 
AAGCCAGCGC CCTTCACCTC CTACCTAGCA CCTTCCAAAG CCTCTAGTCC CACTGTGAGG 
CCTGTTCAGA AGACTTTCAT GACACCCCGA CCTCCAGTCC CCAGCCCCCA GCCCCTGCGC 
CCTACTACTG GCTTATCTAA GAAGTTCACT AACCCCACTG TTGCAAAATC TAAGTCCAAG 
ACGACCAGTT GGGCCAGCAA GCCGGTCTTG GCCCGCTCCA GTGTCCCTAA AACGCTTCAG 
CAAACTGTTT TGTCCCAGTC TCCTGTTTCC TATCTGGGCT CTCAGACACT GGCTCCAGCC 
CTCCCTCCTT TGGGTGTTGG AAATCCTAGA ACGATGCCTC CCACACGTGA CTCTGCTTTA 
ACTCCTGCTG GAAGCAAGAA ATTCACAGGA CGGGAAACCT CCAAGAAAAC CAGACAGAAG 
AGCAGCCCCC GGAAGCCCGA ACCTCTCAGC CCTGGGAAGT CAGCCAGGGA TGCCTCACCA 
AGAGACCTGA CAACCAAGCC TAGCCGGCCG TCCACCCCAG CCCTGGTCCT TGCCCCAGCT 
TACCTCCTGT CCTCCAGCCC CCAGCCCACC AGCAGCAGCT TCCCTTTCTT CCACCTGCTG 
GGCCCTACAC CTTTCCCGAT GCTGATGGGG CCTCCAGGCT CCAAGGGAGA CTGTGGCTTG 
CCGGGTCCCC CTGGGCTTCC AGGATTACCT GGATCTCCGG GCGCACGAGG TCCTCGGGGT 
CCTCCTGGGC CGTACGGGAA CCCAGGCCCA CCTGGCCCTC CAGGAGCCAA AGGACAGAAA 
GGGGACCCAG GACTCTCACC AGGACAGGCT CACGATGGAG CAAAGGGCAA CATGGGCTTG 
CCTGGGCTCT CCGGAAATCC AGGACCCTTG GGAAGGAAGG GACACAAGGG CCATCCTGGA 
GCAGCAGGGC ACCCTGGAGA ACAGGGGCAG CCAGGACCTG AGGGCAGTCC AGGGGCCAAA 
GGTTACCCTG GCAGGCAGGG GTTCCCTGGA CCTGTAGGAG ACCCTGGCCC CAAAGGCAGC 
AGGGGTTACA TTGGTCTTCC GGGGCTCTTT GGCCTGCCAG GGTCGGATGG AGAGCGGGGT 
CTCCCTGGCG TTCCTGGCAA GAGGGGCGAG ATGGGTAGGC CGGGCTTCCC TGGAGACTTT 
GGGGAAAGAG GCCCCCCTGG ACTTGATGGC AACCCTGGAG AAATTGGCCT GCCGGGGCCA 
CCAGGAGTGC TCGGGCTCAT TGGTGACACA GGAGCACTGG GCCCAGTTGG CTACCCAGGA 
CCGAAAGGCA TGAAGGGACT GATGGGCGGC GTGGGGGAGC CCGGACTGAA AGGTGATAAG 
GGTGAACAAG GGGTGCCAGG TGTGTCGGGA GATCCTGGCT TCCAAGGAGA CAAGGGGAGT 
CATGGCTTGC CAGGGCTCCC GGGTGGCCGG GGGAAGCCAG GGCCTCTGGG AAAAGCTGGA 
GACAAAGGAT CACTTGGGTT TCCTGGACCT CCTGGACCTG AGGGTTTCCC AGGAGACATT 
GGCCCTCCAG GGGACAATGG CCCTGAAGGC ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG 
CCTGGACCTC CTGGGCAGCT GGGGCCTGAG GGAGATGAAG GACCCATGGG GCCACCAGGA 
GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT 
GGCTCGAAGG GGGAACCAGG GGATCCTGGA CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT 
ATGGGATTCA TTGGTCTTGT CGGAGAGCCT GGAATTGTTG GGGAGAAGGG AGACCGTGGG 
GTGATGGGAC CCCCAGGTGC ACCTGGACCC AAGGGGTCGA TGGTAAGGAG GCCATCCTGG 
AACACCCGGT GGTATTGGGA ACCCTGGAGA GCCTGGACCC TGGGGCCCTC CAGGATCTCG 
AGGCCTGCCA GGCATGAGGG GAGCCAAGGG GCACCGGGGT CTCCAGCTGA CTTACACAGG 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006538102.1
CDS	507..3692
Translation	MGTGFARGARGTAASGPGGGFLFAWILVSFTCHLASTQGAPEDVDVLQRLGLSWTKAGGGRSPTPPGVIPFPSGFIFTQRAKLQAPTANVLPTTLGRELALVLSLCSHRVNHAFLFAIRSRKHKLQLGLQFLPGRTIIHLGPRQSVAFDLDVHDGRWHHLALELRGRTVTMVTACGQHRVPVPLPSRRDSMLDPQGSFLLGKVNPRAVQFEGALCQFSIHPVAQVAHNYCAHLRERCRQVDTYSPQVGTLFPWDSGPAFALHPEPALLGLGNLTRTPATLGARPVSRALAVTLAPAMPTKPLRTVHPDVSEHSSSQTPLSPAKQSARKTPSPSSSASLANSTRVYRPAAAQPRQITTTSPTKRSPTKPSVSPLSVTPMKSPHATQKTGVPSFTKPVPPTQKPAPFTSYLAPSKASSPTVRPVQKTFMTPRPPVPSPQPLRPTTGLSKKFTNPTVAKSKSKTTSWASKPVLARSSVPKTLQQTVLSQSPVSYLGSQTLAPALPPLGVGNPRTMPPTRDSALTPAGSKKFTGRETSKKTRQKSSPRKPEPLSPGKSARDASPRDLTTKPSRPSTPALVLAPAYLLSSSPQPTSSSFPFFHLLGPTPFPMLMGPPGSKGDCGLPGPPGLPGLPGSPGARGPRGPPGPYGNPGPPGPPGAKGQKGDPGLSPGQAHDGAKGNMGLPGLSGNPGPLGRKGHKGHPGAAGHPGEQGQPGPEGSPGAKGYPGRQGFPGPVGDPGPKGSRGYIGLPGLFGLPGSDGERGLPGVPGKRGEMGRPGFPGDFGERGPPGLDGNPGEIGLPGPPGVLGLIGDTGALGPVGYPGPKGMKGLMGGVGEPGLKGDKGEQGVPGVSGDPGFQGDKGSHGLPGLPGGRGKPGPLGKAGDKGSLGFPGPPGPEGFPGDIGPPGDNGPEGMKGKPGARGLPGPPGQLGPEGDEGPMGPPGVPGLEGQPGRKGFPGRPGLDGSKGEPGDPGRPGPVGEQGLMGFIGLVGEPGIVGEKGDRGVMGPPGAPGPKGSMVRRPSWNTRWYWEPWRAWTLGPSRISRPARHEGSQGAPGSPADLHRL

Target ORF information:

RefSeq Version	XM_006538039.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006538039.4

ORF Insert Sequence:

ATGGGCACGG GATTCGCGCG GGGGGCCCGA GGCACAGCGG CGTCAGGACC CGGGGGGGGG 
TTTCTCTTCG CCTGGATCTT GGTCTCATTT ACCTGTCACC TGGCCTCCAC CCAAGGAGCT 
CCTGAAGATG TGGATGTCCT CCAGCGGCTG GGCCTCAGCT GGACGAAGGC CGGGGGTGGC 
CGGAGTCCTA CACCCCCTGG TGTCATTCCT TTCCCATCTG GCTTCATCTT CACACAGCGG 
GCCAAGCTGC AGGCCCCCAC AGCCAATGTT CTTCCCACCA CCCTGGGGCG GGAGCTGGCA 
TTGGTGTTGA GCCTCTGTTC ACATCGTGTG AACCATGCCT TCCTCTTTGC CATCCGCAGC 
AGGAAACACA AGCTGCAACT GGGTCTGCAG TTCCTCCCCG GCAGGACGAT CATCCACCTG 
GGGCCTCGGC AATCAGTGGC CTTCGACTTG GATGTGCATG ATGGGCGCTG GCACCACCTG 
GCCCTAGAGT TGCGTGGCCG CACAGTCACA ATGGTGACAG CCTGTGGGCA GCACAGGGTA 
CCTGTCCCAC TGCCTTCCCG TAGGGACTCC ATGCTTGACC CCCAGGGCTC CTTTCTCTTG 
GGGAAGGTGA ACCCCCGTGC TGTCCAGTTT GAAGGTGCAC TCTGTCAGTT CAGCATCCAC 
CCTGTGGCGC AGGTCGCTCA CAATTACTGT GCCCACCTGA GAGAGCGGTG CCGACAGGTG 
GACACATATA GTCCCCAGGT GGGAACCCTC TTTCCTTGGG ACTCTGGCCC AGCCTTTGCT 
TTGCATCCTG AACCAGCCTT GCTTGGCTTG GGGAATCTGA CCAGAACTCC AGCAACCCTG 
GGGGCCAGGC CTGTAAGCAG GGCACTTGCA GTGACTCTGG CTCCTGCCAT GCCCACCAAG 
CCCCTGAGGA CTGTCCACCC AGATGTCTCT GAACACAGCT CTTCCCAGAC CCCACTGTCA 
CCTGCCAAAC AGTCGGCCAG GAAAACACCT TCCCCCTCCT CGTCAGCTTC CCTGGCTAAT 
TCCACCAGGG TTTATCGTCC AGCTGCTGCC CAGCCAAGAC AGATCACAAC TACTAGCCCC 
ACCAAACGTT CCCCCACCAA GCCTTCTGTT TCTCCCCTTT CAGTTACCCC CATGAAAAGT 
CCCCATGCAA CCCAGAAAAC AGGTGTCCCT TCATTCACAA AGCCTGTTCC ACCCACTCAG 
AAGCCAGCGC CCTTCACCTC CTACCTAGCA CCTTCCAAAG CCTCTAGTCC CACTGTGAGG 
CCTGTTCAGA AGACTTTCAT GACACCCCGA CCTCCAGTCC CCAGCCCCCA GCCCCTGCGC 
CCTACTACTG GCTTATCTAA GAAGTTCACT AACCCCACTG TTGCAAAATC TAAGTCCAAG 
ACGACCAGTT GGGCCAGCAA GCCGGTCTTG GCCCGCTCCA GTGTCCCTAA AACGCTTCAG 
CAAACTGTTT TGTCCCAGTC TCCTGTTTCC TATCTGGGCT CTCAGACACT GGCTCCAGCC 
CTCCCTCCTT TGGGTGTTGG AAATCCTAGA ACGATGCCTC CCACACGTGA CTCTGCTTTA 
ACTCCTGCTG GAAGCAAGAA ATTCACAGGA CGGGAAACCT CCAAGAAAAC CAGACAGAAG 
AGCAGCCCCC GGAAGCCCGA ACCTCTCAGC CCTGGGAAGT CAGCCAGGGA TGCCTCACCA 
AGAGACCTGA CAACCAAGCC TAGCCGGCCG TCCACCCCAG CCCTGGTCCT TGCCCCAGCT 
TACCTCCTGT CCTCCAGCCC CCAGCCCACC AGCAGCAGCT TCCCTTTCTT CCACCTGCTG 
GGCCCTACAC CTTTCCCGAT GCTGATGGGG CCTCCAGGCT CCAAGGGAGA CTGTGGCTTG 
CCGGGTCCCC CTGGGCTTCC AGGATTACCT GGATCTCCGG GCGCACGAGG TCCTCGGGGT 
CCTCCTGGGC CGTACGGGAA CCCAGGCCCA CCTGGCCCTC CAGGAGCCAA AGGACAGAAA 
GGGGACCCAG GACTCTCACC AGGACAGGCT CACGATGGAG CAAAGGGCAA CATGGGCTTG 
CCTGGGCTCT CCGGAAATCC AGGACCCTTG GGAAGGAAGG GACACAAGGG CCATCCTGGA 
GCAGCAGGGC ACCCTGGAGA ACAGGGGCAG CCAGGACCTG AGGGCAGTCC AGGGGCCAAA 
GGTTACCCTG GCAGGCAGGG GTTCCCTGGA CCTGTAGGAG ACCCTGGCCC CAAAGGCAGC 
AGGGGTTACA TTGGTCTTCC GGGGCTCTTT GGCCTGCCAG GGTCGGATGG AGAGCGGGGT 
CTCCCTGGCG TTCCTGGCAA GAGGGGCGAG ATGGGTAGGC CGGGCTTCCC TGGAGACTTT 
GGGGAAAGAG GCCCCCCTGG ACTTGATGGC AACCCTGGAG AAATTGGCCT GCCGGGGCCA 
CCAGGAGTGC TCGGGCTCAT TGGTGACACA GGAGCACTGG GCCCAGTTGG CTACCCAGGA 
CCGAAAGGCA TGAAGGGACT GATGGGCGGC GTGGGGGAGC CCGGACTGAA AGGTGATAAG 
GGTGAACAAG GGGTGCCAGG TGTGTCGGGA GATCCTGGCT TCCAAGGAGA CAAGGGGAGT 
CATGGCTTGC CAGGGCTCCC GGGTGGCCGG GGGAAGCCAG GGCCTCTGGG AAAAGCTGGA 
GACAAAGGAT CACTTGGGTT TCCTGGACCT CCTGGACCTG AGGGTTTCCC AGGAGACATT 
GGCCCTCCAG GGGACAATGG CCCTGAAGGC ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG 
CCTGGACCTC CTGGGCAGCT GGGGCCTGAG GGAGATGAAG GACCCATGGG GCCACCAGGA 
GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT 
GGCTCGAAGG GGGAACCAGG GGATCCTGGA CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT 
ATGGGATTCA TTGGTCTTGT CGGAGAGCCT GGAATTGTTG GGGAGAAGGG AGACCGTGGG 
GTGATGGGAC CCCCAGGTGC ACCTGGACCC AAGGGGTCGA TGGTAAGGAG GCCATCCTGG 
AACACCCGGT GGTATTGGGA ACCCTGGAGA GCCTGGACCC TGGGGCCCTC CAGGATCTCG 
AGGCCTGCCA GGCATGAGGG GAGCCAAGGG GCACCGGGGT CTCCAGCTGA CTTACACAGG 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col27a1 ( XM_011250057.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011248359.1 Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X6, mRNA.

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CloneID	OMu74976
Clone ID Related Accession (Same CDS sequence)	XM_011250057.3 , XM_011250057.2
Accession Version	XM_011250057.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3708bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	collagen alpha-1(XXVII) chain isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011250057.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCACGG GATTCGCGCG GGGGGCCCGA GGCACAGCGG CGTCAGGACC CGGGGGGGGG 
TTTCTCTTCG CCTGGATCTT GGTCTCATTT ACCTGTCACC TGGCCTCCAC CCAAGGAGCT 
CCTGAAGATG TGGATGTCCT CCAGCGGCTG GGCCTCAGCT GGACGAAGGC CGGGGGTGGC 
CGGAGTCCTA CACCCCCTGG TGTCATTCCT TTCCCATCTG GCTTCATCTT CACACAGCGG 
GCCAAGCTGC AGGCCCCCAC AGCCAATGTT CTTCCCACCA CCCTGGGGCG GGAGCTGGCA 
TTGGTGTTGA GCCTCTGTTC ACATCGTGTG AACCATGCCT TCCTCTTTGC CATCCGCAGC 
AGGAAACACA AGCTGCAACT GGGTCTGCAG TTCCTCCCCG GCAGGACGAT CATCCACCTG 
GGGCCTCGGC AATCAGTGGC CTTCGACTTG GATGTGCATG ATGGGCGCTG GCACCACCTG 
GCCCTAGAGT TGCGTGGCCG CACAGTCACA ATGGTGACAG CCTGTGGGCA GCACAGGGTA 
CCTGTCCCAC TGCCTTCCCG TAGGGACTCC ATGCTTGACC CCCAGGGCTC CTTTCTCTTG 
GGGAAGGTGA ACCCCCGTGC TGTCCAGTTT GAAGGTGCAC TCTGTCAGTT CAGCATCCAC 
CCTGTGGCGC AGGTCGCTCA CAATTACTGT GCCCACCTGA GAGAGCGGTG CCGACAGGTG 
GACACATATA GTCCCCAGGT GGGAACCCTC TTTCCTTGGG ACTCTGGCCC AGCCTTTGCT 
TTGCATCCTG AACCAGCCTT GCTTGGCTTG GGGAATCTGA CCAGAACTCC AGCAACCCTG 
GGGGCCAGGC CTGTAAGCAG GGCACTTGCA GTGACTCTGG CTCCTGCCAT GCCCACCAAG 
CCCCTGAGGA CTGTCCACCC AGATGTCTCT GAACACAGCT CTTCCCAGAC CCCACTGTCA 
CCTGCCAAAC AGTCGGCCAG GAAAACACCT TCCCCCTCCT CGTCAGCTTC CCTGGCTAAT 
TCCACCAGGG TTTATCGTCC AGCTGCTGCC CAGCCAAGAC AGATCACAAC TACTAGCCCC 
ACCAAACGTT CCCCCACCAA GCCTTCTGTT TCTCCCCTTT CAGTTACCCC CATGAAAAGT 
CCCCATGCAA CCCAGAAAAC AGGTGTCCCT TCATTCACAA AGCCTGTTCC ACCCACTCAG 
AAGCCAGCGC CCTTCACCTC CTACCTAGCA CCTTCCAAAG CCTCTAGTCC CACTGTGAGG 
CCTGTTCAGA AGACTTTCAT GACACCCCGA CCTCCAGTCC CCAGCCCCCA GCCCCTGCGC 
CCTACTACTG GCTTATCTAA GAAGTTCACT AACCCCACTG TTGCAAAATC TAAGTCCAAG 
ACGACCAGTT GGGCCAGCAA GCCGGTCTTG GCCCGCTCCA GTGTCCCTAA AACGCTTCAG 
CAAACTGTTT TGTCCCAGTC TCCTGTTTCC TATCTGGGCT CTCAGACACT GGCTCCAGCC 
CTCCCTCCTT TGGGTGTTGG AAATCCTAGA ACGATGCCTC CCACACGTGA CTCTGCTTTA 
ACTCCTGCTG GAAGCAAGAA ATTCACAGGA CGGGAAACCT CCAAGAAAAC CAGACAGAAG 
AGCAGCCCCC GGAAGCCCGA ACCTCTCAGC CCTGGGAAGT CAGCCAGGGA TGCCTCACCA 
AGAGACCTGA CAACCAAGCC TAGCCGGCCG TCCACCCCAG CCCTGGTCCT TGCCCCAGCT 
TACCTCCTGT CCTCCAGCCC CCAGCCCACC AGCAGCAGCT TCCCTTTCTT CCACCTGCTG 
GGCCCTACAC CTTTCCCGAT GCTGATGGGG CCTCCAGGCT CCAAGGGAGA CTGTGGCTTG 
CCGGGTCCCC CTGGGCTTCC AGGATTACCT GGATCTCCGG GCGCACGAGG TCCTCGGGGT 
CCTCCTGGGC CGTACGGGAA CCCAGGCCCA CCTGGCCCTC CAGGAGCCAA AGGACAGAAA 
GGGGACCCAG GACTCTCACC AGGACAGGCT CACGATGGAG CAAAGGGCAA CATGGGCTTG 
CCTGGGCTCT CCGGAAATCC AGGACCCTTG GGAAGGAAGG GACACAAGGG CCATCCTGGA 
GCAGCAGGGC ACCCTGGAGA ACAGGGGCAG CCAGGACCTG AGGGCAGTCC AGGGGCCAAA 
GGTTACCCTG GCAGGCAGGG GTTCCCTGGA CCTGTAGGAG ACCCTGGCCC CAAAGGCAGC 
AGGGGTTACA TTGGTCTTCC GGGGCTCTTT GGCCTGCCAG GGTCGGATGG AGAGCGGGGT 
CTCCCTGGCG TTCCTGGCAA GAGGGGCGAG ATGGGTAGGC CGGGCTTCCC TGGAGACTTT 
GGGGAAAGAG GCCCCCCTGG ACTTGATGGC AACCCTGGAG AAATTGGCCT GCCGGGGCCA 
CCAGGAGTGC TCGGGCTCAT TGGTGACACA GGAGCACTGG GCCCAGTTGG CTACCCAGGA 
CCGAAAGGCA TGAAGGGACT GATGGGCGGC GTGGGGGAGC CCGGACTGAA AGGTGATAAG 
GGTGAACAAG GGGTGCCAGG TGTGTCGGGA GATCCTGGCT TCCAAGGAGA CAAGGGGAGT 
CATGGCTTGC CAGGGCTCCC GGGTGGCCGG GGGAAGCCAG GGCCTCTGGG AAAAGCTGGA 
GACAAAGGAT CACTTGGGTT TCCTGGACCT CCTGGACCTG AGGGTTTCCC AGGAGACATT 
GGCCCTCCAG GGGACAATGG CCCTGAAGGC ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG 
CCTGGACCTC CTGGGCAGCT GGGGCCTGAG GGAGATGAAG GACCCATGGG GCCACCAGGA 
GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT 
GGCTCGAAGG GGGAACCAGG GGATCCTGGA CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT 
ATGGGATTCA TTGGTCTTGT CGGAGAGCCT GGAATTGTTG GGGAGAAGGG AGACCGTGGG 
GTGATGGGAC CCCCAGGTGC ACCTGGACCC AAGGGGTCGA TGGGCCATCC TGGAACACCC 
GGTGGTATTG GGAACCCTGG AGAGCCTGGA CCCTGGGGCC CTCCAGGATC TCGAGGCCTG 
CCAGGCATGA GGGGAGCCAA GGGGCACCGG GGCCCTCGAG GACCTGATGG ACCAGCTGGG 
GAGCAGGGAT CCAAGGGCCT GAAGGGTCGT GTAGGACCTC GGGGCAGACC TGGCCAGCCA 
GGACAGCAGG GTGCAGCTGG TGAGCGAGGC CACTCAGGTG CAAAAGGCTT CCTTGGCATC 
CCTGGTCCCT CAGGCCCTCC AGGTGCCAAG GGCCTCCCAG GAGAACCGGG CTCTCAGGGA 
CCTCAGGGGC CAGTTGGTCC TCCAGGAGAA ATGGGGCCCA AGGGACCGCC GGGTGCAGTG 
GGAGAGCCTG GCCTTCCTGG GGACTCCGGA ATGAAGGGTG ACCTCGGACC TCTGGGCCCC 
CCTGGGGAGC AAGGTCTTAT TGGGCAGCGG GGAGAGCCAG GCCTTGAGGG AGACCATGGC 
CCTGTGGGGC CAGATGGGCT GAAGGGCGAC AGGGGCGACC CAGGGCCCGA TGGCGAGCAT 
GGTGAAAAGG GCCAGGAAGG GCTGAAAGGA GAGGATGGGT CCCCTGGGCC CCCGGGCATC 
ACTGGCGTCC CGATCCATCC ACAGACACTC CAGCTCAGAT GGTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011248359.1
CDS	508..4215
Translation	MGTGFARGARGTAASGPGGGFLFAWILVSFTCHLASTQGAPEDVDVLQRLGLSWTKAGGGRSPTPPGVIPFPSGFIFTQRAKLQAPTANVLPTTLGRELALVLSLCSHRVNHAFLFAIRSRKHKLQLGLQFLPGRTIIHLGPRQSVAFDLDVHDGRWHHLALELRGRTVTMVTACGQHRVPVPLPSRRDSMLDPQGSFLLGKVNPRAVQFEGALCQFSIHPVAQVAHNYCAHLRERCRQVDTYSPQVGTLFPWDSGPAFALHPEPALLGLGNLTRTPATLGARPVSRALAVTLAPAMPTKPLRTVHPDVSEHSSSQTPLSPAKQSARKTPSPSSSASLANSTRVYRPAAAQPRQITTTSPTKRSPTKPSVSPLSVTPMKSPHATQKTGVPSFTKPVPPTQKPAPFTSYLAPSKASSPTVRPVQKTFMTPRPPVPSPQPLRPTTGLSKKFTNPTVAKSKSKTTSWASKPVLARSSVPKTLQQTVLSQSPVSYLGSQTLAPALPPLGVGNPRTMPPTRDSALTPAGSKKFTGRETSKKTRQKSSPRKPEPLSPGKSARDASPRDLTTKPSRPSTPALVLAPAYLLSSSPQPTSSSFPFFHLLGPTPFPMLMGPPGSKGDCGLPGPPGLPGLPGSPGARGPRGPPGPYGNPGPPGPPGAKGQKGDPGLSPGQAHDGAKGNMGLPGLSGNPGPLGRKGHKGHPGAAGHPGEQGQPGPEGSPGAKGYPGRQGFPGPVGDPGPKGSRGYIGLPGLFGLPGSDGERGLPGVPGKRGEMGRPGFPGDFGERGPPGLDGNPGEIGLPGPPGVLGLIGDTGALGPVGYPGPKGMKGLMGGVGEPGLKGDKGEQGVPGVSGDPGFQGDKGSHGLPGLPGGRGKPGPLGKAGDKGSLGFPGPPGPEGFPGDIGPPGDNGPEGMKGKPGARGLPGPPGQLGPEGDEGPMGPPGVPGLEGQPGRKGFPGRPGLDGSKGEPGDPGRPGPVGEQGLMGFIGLVGEPGIVGEKGDRGVMGPPGAPGPKGSMGHPGTPGGIGNPGEPGPWGPPGSRGLPGMRGAKGHRGPRGPDGPAGEQGSKGLKGRVGPRGRPGQPGQQGAAGERGHSGAKGFLGIPGPSGPPGAKGLPGEPGSQGPQGPVGPPGEMGPKGPPGAVGEPGLPGDSGMKGDLGPLGPPGEQGLIGQRGEPGLEGDHGPVGPDGLKGDRGDPGPDGEHGEKGQEGLKGEDGSPGPPGITGVPIHPQTLQLRWS

Target ORF information:

RefSeq Version	XM_011250057.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011250057.3

ORF Insert Sequence:

ATGGGCACGG GATTCGCGCG GGGGGCCCGA GGCACAGCGG CGTCAGGACC CGGGGGGGGG 
TTTCTCTTCG CCTGGATCTT GGTCTCATTT ACCTGTCACC TGGCCTCCAC CCAAGGAGCT 
CCTGAAGATG TGGATGTCCT CCAGCGGCTG GGCCTCAGCT GGACGAAGGC CGGGGGTGGC 
CGGAGTCCTA CACCCCCTGG TGTCATTCCT TTCCCATCTG GCTTCATCTT CACACAGCGG 
GCCAAGCTGC AGGCCCCCAC AGCCAATGTT CTTCCCACCA CCCTGGGGCG GGAGCTGGCA 
TTGGTGTTGA GCCTCTGTTC ACATCGTGTG AACCATGCCT TCCTCTTTGC CATCCGCAGC 
AGGAAACACA AGCTGCAACT GGGTCTGCAG TTCCTCCCCG GCAGGACGAT CATCCACCTG 
GGGCCTCGGC AATCAGTGGC CTTCGACTTG GATGTGCATG ATGGGCGCTG GCACCACCTG 
GCCCTAGAGT TGCGTGGCCG CACAGTCACA ATGGTGACAG CCTGTGGGCA GCACAGGGTA 
CCTGTCCCAC TGCCTTCCCG TAGGGACTCC ATGCTTGACC CCCAGGGCTC CTTTCTCTTG 
GGGAAGGTGA ACCCCCGTGC TGTCCAGTTT GAAGGTGCAC TCTGTCAGTT CAGCATCCAC 
CCTGTGGCGC AGGTCGCTCA CAATTACTGT GCCCACCTGA GAGAGCGGTG CCGACAGGTG 
GACACATATA GTCCCCAGGT GGGAACCCTC TTTCCTTGGG ACTCTGGCCC AGCCTTTGCT 
TTGCATCCTG AACCAGCCTT GCTTGGCTTG GGGAATCTGA CCAGAACTCC AGCAACCCTG 
GGGGCCAGGC CTGTAAGCAG GGCACTTGCA GTGACTCTGG CTCCTGCCAT GCCCACCAAG 
CCCCTGAGGA CTGTCCACCC AGATGTCTCT GAACACAGCT CTTCCCAGAC CCCACTGTCA 
CCTGCCAAAC AGTCGGCCAG GAAAACACCT TCCCCCTCCT CGTCAGCTTC CCTGGCTAAT 
TCCACCAGGG TTTATCGTCC AGCTGCTGCC CAGCCAAGAC AGATCACAAC TACTAGCCCC 
ACCAAACGTT CCCCCACCAA GCCTTCTGTT TCTCCCCTTT CAGTTACCCC CATGAAAAGT 
CCCCATGCAA CCCAGAAAAC AGGTGTCCCT TCATTCACAA AGCCTGTTCC ACCCACTCAG 
AAGCCAGCGC CCTTCACCTC CTACCTAGCA CCTTCCAAAG CCTCTAGTCC CACTGTGAGG 
CCTGTTCAGA AGACTTTCAT GACACCCCGA CCTCCAGTCC CCAGCCCCCA GCCCCTGCGC 
CCTACTACTG GCTTATCTAA GAAGTTCACT AACCCCACTG TTGCAAAATC TAAGTCCAAG 
ACGACCAGTT GGGCCAGCAA GCCGGTCTTG GCCCGCTCCA GTGTCCCTAA AACGCTTCAG 
CAAACTGTTT TGTCCCAGTC TCCTGTTTCC TATCTGGGCT CTCAGACACT GGCTCCAGCC 
CTCCCTCCTT TGGGTGTTGG AAATCCTAGA ACGATGCCTC CCACACGTGA CTCTGCTTTA 
ACTCCTGCTG GAAGCAAGAA ATTCACAGGA CGGGAAACCT CCAAGAAAAC CAGACAGAAG 
AGCAGCCCCC GGAAGCCCGA ACCTCTCAGC CCTGGGAAGT CAGCCAGGGA TGCCTCACCA 
AGAGACCTGA CAACCAAGCC TAGCCGGCCG TCCACCCCAG CCCTGGTCCT TGCCCCAGCT 
TACCTCCTGT CCTCCAGCCC CCAGCCCACC AGCAGCAGCT TCCCTTTCTT CCACCTGCTG 
GGCCCTACAC CTTTCCCGAT GCTGATGGGG CCTCCAGGCT CCAAGGGAGA CTGTGGCTTG 
CCGGGTCCCC CTGGGCTTCC AGGATTACCT GGATCTCCGG GCGCACGAGG TCCTCGGGGT 
CCTCCTGGGC CGTACGGGAA CCCAGGCCCA CCTGGCCCTC CAGGAGCCAA AGGACAGAAA 
GGGGACCCAG GACTCTCACC AGGACAGGCT CACGATGGAG CAAAGGGCAA CATGGGCTTG 
CCTGGGCTCT CCGGAAATCC AGGACCCTTG GGAAGGAAGG GACACAAGGG CCATCCTGGA 
GCAGCAGGGC ACCCTGGAGA ACAGGGGCAG CCAGGACCTG AGGGCAGTCC AGGGGCCAAA 
GGTTACCCTG GCAGGCAGGG GTTCCCTGGA CCTGTAGGAG ACCCTGGCCC CAAAGGCAGC 
AGGGGTTACA TTGGTCTTCC GGGGCTCTTT GGCCTGCCAG GGTCGGATGG AGAGCGGGGT 
CTCCCTGGCG TTCCTGGCAA GAGGGGCGAG ATGGGTAGGC CGGGCTTCCC TGGAGACTTT 
GGGGAAAGAG GCCCCCCTGG ACTTGATGGC AACCCTGGAG AAATTGGCCT GCCGGGGCCA 
CCAGGAGTGC TCGGGCTCAT TGGTGACACA GGAGCACTGG GCCCAGTTGG CTACCCAGGA 
CCGAAAGGCA TGAAGGGACT GATGGGCGGC GTGGGGGAGC CCGGACTGAA AGGTGATAAG 
GGTGAACAAG GGGTGCCAGG TGTGTCGGGA GATCCTGGCT TCCAAGGAGA CAAGGGGAGT 
CATGGCTTGC CAGGGCTCCC GGGTGGCCGG GGGAAGCCAG GGCCTCTGGG AAAAGCTGGA 
GACAAAGGAT CACTTGGGTT TCCTGGACCT CCTGGACCTG AGGGTTTCCC AGGAGACATT 
GGCCCTCCAG GGGACAATGG CCCTGAAGGC ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG 
CCTGGACCTC CTGGGCAGCT GGGGCCTGAG GGAGATGAAG GACCCATGGG GCCACCAGGA 
GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT 
GGCTCGAAGG GGGAACCAGG GGATCCTGGA CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT 
ATGGGATTCA TTGGTCTTGT CGGAGAGCCT GGAATTGTTG GGGAGAAGGG AGACCGTGGG 
GTGATGGGAC CCCCAGGTGC ACCTGGACCC AAGGGGTCGA TGGGCCATCC TGGAACACCC 
GGTGGTATTG GGAACCCTGG AGAGCCTGGA CCCTGGGGCC CTCCAGGATC TCGAGGCCTG 
CCAGGCATGA GGGGAGCCAA GGGGCACCGG GGCCCTCGAG GACCTGATGG ACCAGCTGGG 
GAGCAGGGAT CCAAGGGCCT GAAGGGTCGT GTAGGACCTC GGGGCAGACC TGGCCAGCCA 
GGACAGCAGG GTGCAGCTGG TGAGCGAGGC CACTCAGGTG CAAAAGGCTT CCTTGGCATC 
CCTGGTCCCT CAGGCCCTCC AGGTGCCAAG GGCCTCCCAG GAGAACCGGG CTCTCAGGGA 
CCTCAGGGGC CAGTTGGTCC TCCAGGAGAA ATGGGGCCCA AGGGACCGCC GGGTGCAGTG 
GGAGAGCCTG GCCTTCCTGG GGACTCCGGA ATGAAGGGTG ACCTCGGACC TCTGGGCCCC 
CCTGGGGAGC AAGGTCTTAT TGGGCAGCGG GGAGAGCCAG GCCTTGAGGG AGACCATGGC 
CCTGTGGGGC CAGATGGGCT GAAGGGCGAC AGGGGCGACC CAGGGCCCGA TGGCGAGCAT 
GGTGAAAAGG GCCAGGAAGG GCTGAAAGGA GAGGATGGGT CCCCTGGGCC CCCGGGCATC 
ACTGGCGTCC CGATCCATCC ACAGACACTC CAGCTCAGAT GGTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col27a1 ( XM_011250058.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011248360.1 Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X9, mRNA.

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CloneID	OMu74977
Clone ID Related Accession (Same CDS sequence)	XM_011250058.3 , XM_011250058.2
Accession Version	XM_011250058.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3186bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	collagen alpha-1(XXVII) chain isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011250058.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCACGG GATTCGCGCG GGGGGCCCGA GGCACAGCGG CGTCAGGACC CGGGGGGGGG 
TTTCTCTTCG CCTGGATCTT GGTCTCATTT ACCTGTCACC TGGCCTCCAC CCAAGGAGCT 
CCTGAAGATG TGGATGTCCT CCAGCGGCTG GGCCTCAGCT GGACGAAGGC CGGGGGTGGC 
CGGAGTCCTA CACCCCCTGG TGTCATTCCT TTCCCATCTG GCTTCATCTT CACACAGCGG 
GCCAAGCTGC AGGCCCCCAC AGCCAATGTT CTTCCCACCA CCCTGGGGCG GGAGCTGGCA 
TTGGTGTTGA GCCTCTGTTC ACATCGTGTG AACCATGCCT TCCTCTTTGC CATCCGCAGC 
AGGAAACACA AGCTGCAACT GGGTCTGCAG TTCCTCCCCG GCAGGACGAT CATCCACCTG 
GGGCCTCGGC AATCAGTGGC CTTCGACTTG GATGTGCATG ATGGGCGCTG GCACCACCTG 
GCCCTAGAGT TGCGTGGCCG CACAGTCACA ATGGTGACAG CCTGTGGGCA GCACAGGGTA 
CCTGTCCCAC TGCCTTCCCG TAGGGACTCC ATGCTTGACC CCCAGGGCTC CTTTCTCTTG 
GGGAAGGTGA ACCCCCGTGC TGTCCAGTTT GAAGGTGCAC TCTGTCAGTT CAGCATCCAC 
CCTGTGGCGC AGGTCGCTCA CAATTACTGT GCCCACCTGA GAGAGCGGTG CCGACAGGTG 
GACACATATA GTCCCCAGGT GGGAACCCTC TTTCCTTGGG ACTCTGGCCC AGCCTTTGCT 
TTGCATCCTG AACCAGCCTT GCTTGGCTTG GGGAATCTGA CCAGAACTCC AGCAACCCTG 
GGGGCCAGGC CTGTAAGCAG GGCACTTGCA GTGACTCTGG CTCCTGCCAT GCCCACCAAG 
CCCCTGAGGA CTGTCCACCC AGATGTCTCT GAACACAGCT CTTCCCAGAC CCCACTGTCA 
CCTGCCAAAC AGTCGGCCAG GAAAACACCT TCCCCCTCCT CGTCAGCTTC CCTGGCTAAT 
TCCACCAGGG TTTATCGTCC AGCTGCTGCC CAGCCAAGAC AGATCACAAC TACTAGCCCC 
ACCAAACGTT CCCCCACCAA GCCTTCTGTT TCTCCCCTTT CAGTTACCCC CATGAAAAGT 
CCCCATGCAA CCCAGAAAAC AGGTGTCCCT TCATTCACAA AGCCTGTTCC ACCCACTCAG 
AAGCCAGCGC CCTTCACCTC CTACCTAGCA CCTTCCAAAG CCTCTAGTCC CACTGTGAGG 
CCTGTTCAGA AGACTTTCAT GACACCCCGA CCTCCAGTCC CCAGCCCCCA GCCCCTGCGC 
CCTACTACTG GCTTATCTAA GAAGTTCACT AACCCCACTG TTGCAAAATC TAAGTCCAAG 
ACGACCAGTT GGGCCAGCAA GCCGGTCTTG GCCCGCTCCA GTGTCCCTAA AACGCTTCAG 
CAAACTGTTT TGTCCCAGTC TCCTGTTTCC TATCTGGGCT CTCAGACACT GGCTCCAGCC 
CTCCCTCCTT TGGGTGTTGG AAATCCTAGA ACGATGCCTC CCACACGTGA CTCTGCTTTA 
ACTCCTGCTG GAAGCAAGAA ATTCACAGGA CGGGAAACCT CCAAGAAAAC CAGACAGAAG 
AGCAGCCCCC GGAAGCCCGA ACCTCTCAGC CCTGGGAAGT CAGCCAGGGA TGCCTCACCA 
AGAGACCTGA CAACCAAGCC TAGCCGGCCG TCCACCCCAG CCCTGGTCCT TGCCCCAGCT 
TACCTCCTGT CCTCCAGCCC CCAGCCCACC AGCAGCAGCT TCCCTTTCTT CCACCTGCTG 
GGCCCTACAC CTTTCCCGAT GCTGATGGGG CCTCCAGGCT CCAAGGGAGA CTGTGGCTTG 
CCGGGTCCCC CTGGGCTTCC AGGATTACCT GGATCTCCGG GCGCACGAGG TCCTCGGGGT 
CCTCCTGGGC CGTACGGGAA CCCAGGCCCA CCTGGCCCTC CAGGAGCCAA AGGACAGAAA 
GGGGACCCAG GACTCTCACC AGGACAGGCT CACGATGGAG CAAAGGGCAA CATGGGCTTG 
CCTGGGCTCT CCGGAAATCC AGGACCCTTG GGAAGGAAGG GACACAAGGG CCATCCTGGA 
GCAGCAGGGC ACCCTGGAGA ACAGGGGCAG CCAGGACCTG AGGGCAGTCC AGGGGCCAAA 
GGTTACCCTG GCAGGCAGGG GTTCCCTGGA CCTGTAGGAG ACCCTGGCCC CAAAGGCAGC 
AGGGGTTACA TTGGTCTTCC GGGGCTCTTT GGCCTGCCAG GGTCGGATGG AGAGCGGGGT 
CTCCCTGGCG TTCCTGGCAA GAGGGGCGAG ATGGGTAGGC CGGGCTTCCC TGGAGACTTT 
GGGGAAAGAG GCCCCCCTGG ACTTGATGGC AACCCTGGAG AAATTGGCCT GCCGGGGCCA 
CCAGGAGTGC TCGGGCTCAT TGGTGACACA GGAGCACTGG GCCCAGTTGG CTACCCAGGA 
CCGAAAGGCA TGAAGGGACT GATGGGCGGC GTGGGGGAGC CCGGACTGAA AGGTGATAAG 
GGTGAACAAG GGGTGCCAGG TGTGTCGGGA GATCCTGGCT TCCAAGGAGA CAAGGGGAGT 
CATGGCTTGC CAGGGCTCCC GGGTGGCCGG GGGAAGCCAG GGCCTCTGGG AAAAGCTGGA 
GACAAAGGAT CACTTGGGTT TCCTGGACCT CCTGGACCTG AGGGTTTCCC AGGAGACATT 
GGCCCTCCAG GGGACAATGG CCCTGAAGGC ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG 
CCTGGACCTC CTGGGCAGCT GGGGCCTGAG GGAGATGAAG GACCCATGGG GCCACCAGGA 
GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT 
GGCTCGAAGG GGGAACCAGG GGATCCTGGA CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT 
ATGGGATTCA TTGGTCTTGT CGGAGAGCCT GGAATTGTTG GGGAGAAGGG AGACCGTGGG 
GTGATGGGAC CCCCAGGTGC ACCTGGACCC AAGGGGTCGA TGGGCCATCC TGGAACACCC 
GGTGGTATTG GGAACCCTGG AGAGCCTGGA CCCTGGGGCC CTCCAGGATC TCGAGGCCTG 
CCAGGCATGA GGGGAGCCAA GGGGCACCGG GGTCTCCAGC TGACTTACAC AGGCTGTGAC 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011248360.1
CDS	507..3692
Translation	MGTGFARGARGTAASGPGGGFLFAWILVSFTCHLASTQGAPEDVDVLQRLGLSWTKAGGGRSPTPPGVIPFPSGFIFTQRAKLQAPTANVLPTTLGRELALVLSLCSHRVNHAFLFAIRSRKHKLQLGLQFLPGRTIIHLGPRQSVAFDLDVHDGRWHHLALELRGRTVTMVTACGQHRVPVPLPSRRDSMLDPQGSFLLGKVNPRAVQFEGALCQFSIHPVAQVAHNYCAHLRERCRQVDTYSPQVGTLFPWDSGPAFALHPEPALLGLGNLTRTPATLGARPVSRALAVTLAPAMPTKPLRTVHPDVSEHSSSQTPLSPAKQSARKTPSPSSSASLANSTRVYRPAAAQPRQITTTSPTKRSPTKPSVSPLSVTPMKSPHATQKTGVPSFTKPVPPTQKPAPFTSYLAPSKASSPTVRPVQKTFMTPRPPVPSPQPLRPTTGLSKKFTNPTVAKSKSKTTSWASKPVLARSSVPKTLQQTVLSQSPVSYLGSQTLAPALPPLGVGNPRTMPPTRDSALTPAGSKKFTGRETSKKTRQKSSPRKPEPLSPGKSARDASPRDLTTKPSRPSTPALVLAPAYLLSSSPQPTSSSFPFFHLLGPTPFPMLMGPPGSKGDCGLPGPPGLPGLPGSPGARGPRGPPGPYGNPGPPGPPGAKGQKGDPGLSPGQAHDGAKGNMGLPGLSGNPGPLGRKGHKGHPGAAGHPGEQGQPGPEGSPGAKGYPGRQGFPGPVGDPGPKGSRGYIGLPGLFGLPGSDGERGLPGVPGKRGEMGRPGFPGDFGERGPPGLDGNPGEIGLPGPPGVLGLIGDTGALGPVGYPGPKGMKGLMGGVGEPGLKGDKGEQGVPGVSGDPGFQGDKGSHGLPGLPGGRGKPGPLGKAGDKGSLGFPGPPGPEGFPGDIGPPGDNGPEGMKGKPGARGLPGPPGQLGPEGDEGPMGPPGVPGLEGQPGRKGFPGRPGLDGSKGEPGDPGRPGPVGEQGLMGFIGLVGEPGIVGEKGDRGVMGPPGAPGPKGSMGHPGTPGGIGNPGEPGPWGPPGSRGLPGMRGAKGHRGLQLTYTGCDL

Target ORF information:

RefSeq Version	XM_011250058.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011250058.3

ORF Insert Sequence:

ATGGGCACGG GATTCGCGCG GGGGGCCCGA GGCACAGCGG CGTCAGGACC CGGGGGGGGG 
TTTCTCTTCG CCTGGATCTT GGTCTCATTT ACCTGTCACC TGGCCTCCAC CCAAGGAGCT 
CCTGAAGATG TGGATGTCCT CCAGCGGCTG GGCCTCAGCT GGACGAAGGC CGGGGGTGGC 
CGGAGTCCTA CACCCCCTGG TGTCATTCCT TTCCCATCTG GCTTCATCTT CACACAGCGG 
GCCAAGCTGC AGGCCCCCAC AGCCAATGTT CTTCCCACCA CCCTGGGGCG GGAGCTGGCA 
TTGGTGTTGA GCCTCTGTTC ACATCGTGTG AACCATGCCT TCCTCTTTGC CATCCGCAGC 
AGGAAACACA AGCTGCAACT GGGTCTGCAG TTCCTCCCCG GCAGGACGAT CATCCACCTG 
GGGCCTCGGC AATCAGTGGC CTTCGACTTG GATGTGCATG ATGGGCGCTG GCACCACCTG 
GCCCTAGAGT TGCGTGGCCG CACAGTCACA ATGGTGACAG CCTGTGGGCA GCACAGGGTA 
CCTGTCCCAC TGCCTTCCCG TAGGGACTCC ATGCTTGACC CCCAGGGCTC CTTTCTCTTG 
GGGAAGGTGA ACCCCCGTGC TGTCCAGTTT GAAGGTGCAC TCTGTCAGTT CAGCATCCAC 
CCTGTGGCGC AGGTCGCTCA CAATTACTGT GCCCACCTGA GAGAGCGGTG CCGACAGGTG 
GACACATATA GTCCCCAGGT GGGAACCCTC TTTCCTTGGG ACTCTGGCCC AGCCTTTGCT 
TTGCATCCTG AACCAGCCTT GCTTGGCTTG GGGAATCTGA CCAGAACTCC AGCAACCCTG 
GGGGCCAGGC CTGTAAGCAG GGCACTTGCA GTGACTCTGG CTCCTGCCAT GCCCACCAAG 
CCCCTGAGGA CTGTCCACCC AGATGTCTCT GAACACAGCT CTTCCCAGAC CCCACTGTCA 
CCTGCCAAAC AGTCGGCCAG GAAAACACCT TCCCCCTCCT CGTCAGCTTC CCTGGCTAAT 
TCCACCAGGG TTTATCGTCC AGCTGCTGCC CAGCCAAGAC AGATCACAAC TACTAGCCCC 
ACCAAACGTT CCCCCACCAA GCCTTCTGTT TCTCCCCTTT CAGTTACCCC CATGAAAAGT 
CCCCATGCAA CCCAGAAAAC AGGTGTCCCT TCATTCACAA AGCCTGTTCC ACCCACTCAG 
AAGCCAGCGC CCTTCACCTC CTACCTAGCA CCTTCCAAAG CCTCTAGTCC CACTGTGAGG 
CCTGTTCAGA AGACTTTCAT GACACCCCGA CCTCCAGTCC CCAGCCCCCA GCCCCTGCGC 
CCTACTACTG GCTTATCTAA GAAGTTCACT AACCCCACTG TTGCAAAATC TAAGTCCAAG 
ACGACCAGTT GGGCCAGCAA GCCGGTCTTG GCCCGCTCCA GTGTCCCTAA AACGCTTCAG 
CAAACTGTTT TGTCCCAGTC TCCTGTTTCC TATCTGGGCT CTCAGACACT GGCTCCAGCC 
CTCCCTCCTT TGGGTGTTGG AAATCCTAGA ACGATGCCTC CCACACGTGA CTCTGCTTTA 
ACTCCTGCTG GAAGCAAGAA ATTCACAGGA CGGGAAACCT CCAAGAAAAC CAGACAGAAG 
AGCAGCCCCC GGAAGCCCGA ACCTCTCAGC CCTGGGAAGT CAGCCAGGGA TGCCTCACCA 
AGAGACCTGA CAACCAAGCC TAGCCGGCCG TCCACCCCAG CCCTGGTCCT TGCCCCAGCT 
TACCTCCTGT CCTCCAGCCC CCAGCCCACC AGCAGCAGCT TCCCTTTCTT CCACCTGCTG 
GGCCCTACAC CTTTCCCGAT GCTGATGGGG CCTCCAGGCT CCAAGGGAGA CTGTGGCTTG 
CCGGGTCCCC CTGGGCTTCC AGGATTACCT GGATCTCCGG GCGCACGAGG TCCTCGGGGT 
CCTCCTGGGC CGTACGGGAA CCCAGGCCCA CCTGGCCCTC CAGGAGCCAA AGGACAGAAA 
GGGGACCCAG GACTCTCACC AGGACAGGCT CACGATGGAG CAAAGGGCAA CATGGGCTTG 
CCTGGGCTCT CCGGAAATCC AGGACCCTTG GGAAGGAAGG GACACAAGGG CCATCCTGGA 
GCAGCAGGGC ACCCTGGAGA ACAGGGGCAG CCAGGACCTG AGGGCAGTCC AGGGGCCAAA 
GGTTACCCTG GCAGGCAGGG GTTCCCTGGA CCTGTAGGAG ACCCTGGCCC CAAAGGCAGC 
AGGGGTTACA TTGGTCTTCC GGGGCTCTTT GGCCTGCCAG GGTCGGATGG AGAGCGGGGT 
CTCCCTGGCG TTCCTGGCAA GAGGGGCGAG ATGGGTAGGC CGGGCTTCCC TGGAGACTTT 
GGGGAAAGAG GCCCCCCTGG ACTTGATGGC AACCCTGGAG AAATTGGCCT GCCGGGGCCA 
CCAGGAGTGC TCGGGCTCAT TGGTGACACA GGAGCACTGG GCCCAGTTGG CTACCCAGGA 
CCGAAAGGCA TGAAGGGACT GATGGGCGGC GTGGGGGAGC CCGGACTGAA AGGTGATAAG 
GGTGAACAAG GGGTGCCAGG TGTGTCGGGA GATCCTGGCT TCCAAGGAGA CAAGGGGAGT 
CATGGCTTGC CAGGGCTCCC GGGTGGCCGG GGGAAGCCAG GGCCTCTGGG AAAAGCTGGA 
GACAAAGGAT CACTTGGGTT TCCTGGACCT CCTGGACCTG AGGGTTTCCC AGGAGACATT 
GGCCCTCCAG GGGACAATGG CCCTGAAGGC ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG 
CCTGGACCTC CTGGGCAGCT GGGGCCTGAG GGAGATGAAG GACCCATGGG GCCACCAGGA 
GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT 
GGCTCGAAGG GGGAACCAGG GGATCCTGGA CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT 
ATGGGATTCA TTGGTCTTGT CGGAGAGCCT GGAATTGTTG GGGAGAAGGG AGACCGTGGG 
GTGATGGGAC CCCCAGGTGC ACCTGGACCC AAGGGGTCGA TGGGCCATCC TGGAACACCC 
GGTGGTATTG GGAACCCTGG AGAGCCTGGA CCCTGGGGCC CTCCAGGATC TCGAGGCCTG 
CCAGGCATGA GGGGAGCCAA GGGGCACCGG GGTCTCCAGC TGACTTACAC AGGCTGTGAC 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col27a1 ( XM_017320285.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175774.1 Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OMu81039
Clone ID Related Accession (Same CDS sequence)	XM_017320285.1
Accession Version	XM_017320285.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	collagen alpha-1(XXVII) chain isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGCG GGGTCTCCCT GGCGTTCCTG GCAAGAGGGG CGAGATGGGG CTTCCCTGGA 
GACTTTGGGG AAAGAGGCCC CCCTGGACTT GATGGCAACC CTGGAGAAAT TGGCCTGCCG 
GGGCCACCAG GAGTGCTCGG GCTCATTGGT GACACAGGAG CACTGGGCCC AGTTGGCTAC 
CCAGGACCGA AAGGCATGAA GGGACTGATG GGCGGCGTGG GGGAGCCCGG ACTGAAAGGT 
GATAAGGGTG AACAAGGGGT GCCAGGTGTG TCGGGAGATC CTGGCTTCCA AGGAGACAAG 
GGGAGTCATG GCTTGCCAGG GCTCCCGGGT GGCCGGGGGA AGCCAGGGCC TCTGGGAAAA 
GCTGGAGACA AAGGATCACT TGGGTTTCCT GGACCTCCTG GACCTGAGGG TTTCCCAGGA 
GACATTGGCC CTCCAGGGGA CAATGGCCCT GAAGGCATGA AGGGTAAGCC TGGAGCCCGA 
GGCCTGCCTG GACCTCCTGG GCAGCTGGGG CCTGAGGGAG ATGAAGGACC CATGGGGCCA 
CCAGGAGTCC CAGGCTTGGA GGGTCAGCCT GGAAGGAAAG GGTTTCCTGG GAGGCCTGGC 
CTGGATGGCT CGAAGGGGGA ACCAGGGGAT CCTGGACGGC CAGGACCTGT GGGTGAACAG 
GGGCTTATGG GATTCATTGG TCTTGTCGGA GAGCCTGGAA TTGTTGGGGA GAAGGGAGAC 
CGTGGGGTGA TGGGACCCCC AGGTGCACCT GGACCCAAGG GGTCGATGGG CCATCCTGGA 
ACACCCGGTG GTATTGGGAA CCCTGGAGAG CCTGGACCCT GGGGCCCTCC AGGATCTCGA 
GGCCTGCCAG GCATGAGGGG AGCCAAGGGG CACCGGGGCC CTCGAGGACC TGATGGACCA 
GCTGGGGAGC AGGGATCCAA GGGCCTGAAG GGTCGTGTAG GACCTCGGGG CAGACCTGGC 
CAGCCAGGAC AGCAGGGTGC AGCTGGTGAG CGAGGCCACT CAGGTGCAAA AGGCTTCCTT 
GGCATCCCTG GTCCCTCAGG CCCTCCAGGT GCCAAGGGCC TCCCAGGAGA ACCGGGCTCT 
CAGGGACCTC AGGGGCCAGT TGGTCCTCCA GGAGAAATGG GGCCCAAGGG ACCGCCGGGT 
GCAGTGGGAG AGCCTGGCCT TCCTGGGGAC TCCGGAATGA AGGGTGACCT CGGACCTCTG 
GGCCCCCCTG GGGAGCAAGG TCTTATTGGG CAGCGGGGAG AGCCAGGCCT TGAGGGAGAC 
CATGGCCCTG TGGGGCCAGA TGGGCTGAAG GGCGACAGGG GCGACCCAGG GCCCGATGGC 
GAGCATGGTG AAAAGGGCCA GGAAGGGCTG AAAGGAGAGG ATGGGTCCCC TGGGCCCCCG 
GGCATCACTG GCGTCCCGGG TCGTGAGGGG AAGCCGGGCA AGCAGGGTGA GAAAGGCCAG 
AGGGGAGCTA AGGGTGCCAA GGGCCACCAA GGATATCTGG GAGAGATGGG CATCCCCGGA 
GAACCTGGGC CCCCTGGGAC CCCAGGTCCT AAAGGGTCCC GGGGCACCCT AGGACCAACG 
GGTGCTCCAG GACGGATGGG AGCCCAAGGA GAACCAGGAT TGGCCGGTTA TAATGGACAC 
AAAGGCATCA CAGGACCCCT CGGGCCTCCC GGGCCCAAAG GCGAGAAGGG GGACCAGGGC 
GAGGATGGCA AGACCGAGGG GCCCCCTGGG CCACCAGGAG ATCGGGGTCC TGTGGGTGAT 
CGAGGAGACC GTGGGGAGCC AGGCGACCCT GGATACCCTG GTCAAGAGGG AGTTCAAGGC 
CTCCGTGGAG AACCGGGCCA GCAGGGACAG CCTGGGCATC CAGGACCCCG GGGGCGCCCA 
GGACCCAAAG GATCAAAAGG CGAAGAGGGC CCAAAGGGAA AGCCAGGCAA GGCTGGGCCA 
TCAGGCCGGA GGGGGACCCA GGTGAGTTGC CATAGCTTCT CTGTCACCTT CTATGTTCTC 
CTGAGAAGGT TTAACCAGCT TCTTCTGTAC CTCCAGGGCC TTCAAGGACT GCCAGGCCCC 
CGAGGCGTGG TGGGGAGACA GGGCCCTGAG GGCACTGCTG GATCTGATGG GATTCCTGGC 
AGAGATGGTC GGCCAGGATA TCAGGGAGAC CAGGGAAATG ATGGGGACCC TGGCCCTGTG 
GGCCCTGCTG GGAGAAGAGG AAATCCAGGT GTGGCTGGCT TGCCTGGAGC ACAGGGGCCT 
CCGGGATTCA AGGGTGAAAG TGGGTTACCT GGGCAACTGG GTCCCCCTGG AAAACGAGGG 
ACAGAAGGTG GAACAGGGCT TCCTGGGAAC CAGGGGGAGC CAGGATCCAA AGGCCAGCCG 
GGTGACTCTG GCGAGATGGG CTTCCCAGGA GTGGCTGGCC TCTTTGGACC CAAGGGCCCC 
CCTGGAGACA TTGGCTTCAA AGGCATCCAA GGTCCTCGGG GGCCTCCTGG CTTGATGGGA 
AAGGAAGGTA TCATTGGCCC CCCCGGAATG CTGGGACCTT CTGGACTCCC GGGTCCCAAA 
GGTGACAGAG GCAGCCGAGG AGACTTGGGA CTGCAAGGCC CAAGGGGTCC TCCTGGTCCA 
AGGGGCCGGC CAGGTCCCCC GGGCCCTCCT TGGCATCCCA TCCAGTTTCA GCAAGATGAC 
CTTGGAGCAG CTTTCCAGAC ATGGATGGAT GCTCAAGGAG CCGTCAGATC GGAGCAGGGG 
TACAGCTATC CGGACCAGCT GGCGCTAGAC CAGGGAGGGG AGATCTTCAA AACCTTACAC 
TACCTCAGCA ACCTCATCCA GAGCATTAAG ACACCCTTGG GCACCAAGGA GAACCCAGCC 
CGGGTCTGCC GGGACCTCAT GGACTGTGAG CAGAGGATGG CGGATGGTAC CTACTGGGTG 
GACCCCAACC TCGGCTGCTC CTCTGACACC ATTGAAGTCT CCTGCAACTT TACACAGGGT 
GGGCAGACGT GCCTGAAGCC CATCACGGCC TCCAAGGCCG AGTTTGCTGT GAGTCGGGTC 
CAGATGAATT TCTTGCACCT GCTGAGCTCT GAGGGGACAC AGCATATCAC AATCCACTGT 
CTGAACATGA CGGTGTGGCA GGAGGGACCG GGACGCTCCT CTGCCAGACA GGCTGTGCGC 
TTCCGTGCCT GGAACGGACA GGTCTTCGAA GCTGGGGGTC AGTTCAGGCC AGAGGTGTCT 
ATGGATGGCT GCAAGGTCCA TGATGGCCGC TGGCATCAGA CACTGTTCAC CTTCCGGACC 
CAGGACCCCC AGCAGCTGCC CATCGTCAGT GTGGACAATC TCCCGCCTGT CTCATCAGGG 
AAGCAGTACC GCCTGGAAGT TGGACCTGCG TGCTTCCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175774.1
CDS	90..3431
Translation	MESGVSLAFLARGARWGFPGDFGERGPPGLDGNPGEIGLPGPPGVLGLIGDTGALGPVGYPGPKGMKGLMGGVGEPGLKGDKGEQGVPGVSGDPGFQGDKGSHGLPGLPGGRGKPGPLGKAGDKGSLGFPGPPGPEGFPGDIGPPGDNGPEGMKGKPGARGLPGPPGQLGPEGDEGPMGPPGVPGLEGQPGRKGFPGRPGLDGSKGEPGDPGRPGPVGEQGLMGFIGLVGEPGIVGEKGDRGVMGPPGAPGPKGSMGHPGTPGGIGNPGEPGPWGPPGSRGLPGMRGAKGHRGPRGPDGPAGEQGSKGLKGRVGPRGRPGQPGQQGAAGERGHSGAKGFLGIPGPSGPPGAKGLPGEPGSQGPQGPVGPPGEMGPKGPPGAVGEPGLPGDSGMKGDLGPLGPPGEQGLIGQRGEPGLEGDHGPVGPDGLKGDRGDPGPDGEHGEKGQEGLKGEDGSPGPPGITGVPGREGKPGKQGEKGQRGAKGAKGHQGYLGEMGIPGEPGPPGTPGPKGSRGTLGPTGAPGRMGAQGEPGLAGYNGHKGITGPLGPPGPKGEKGDQGEDGKTEGPPGPPGDRGPVGDRGDRGEPGDPGYPGQEGVQGLRGEPGQQGQPGHPGPRGRPGPKGSKGEEGPKGKPGKAGPSGRRGTQVSCHSFSVTFYVLLRRFNQLLLYLQGLQGLPGPRGVVGRQGPEGTAGSDGIPGRDGRPGYQGDQGNDGDPGPVGPAGRRGNPGVAGLPGAQGPPGFKGESGLPGQLGPPGKRGTEGGTGLPGNQGEPGSKGQPGDSGEMGFPGVAGLFGPKGPPGDIGFKGIQGPRGPPGLMGKEGIIGPPGMLGPSGLPGPKGDRGSRGDLGLQGPRGPPGPRGRPGPPGPPWHPIQFQQDDLGAAFQTWMDAQGAVRSEQGYSYPDQLALDQGGEIFKTLHYLSNLIQSIKTPLGTKENPARVCRDLMDCEQRMADGTYWVDPNLGCSSDTIEVSCNFTQGGQTCLKPITASKAEFAVSRVQMNFLHLLSSEGTQHITIHCLNMTVWQEGPGRSSARQAVRFRAWNGQVFEAGGQFRPEVSMDGCKVHDGRWHQTLFTFRTQDPQQLPIVSVDNLPPVSSGKQYRLEVGPACFL

Target ORF information:

RefSeq Version	XM_017320285.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320285.1

ORF Insert Sequence:

ATGGAGAGCG GGGTCTCCCT GGCGTTCCTG GCAAGAGGGG CGAGATGGGG CTTCCCTGGA 
GACTTTGGGG AAAGAGGCCC CCCTGGACTT GATGGCAACC CTGGAGAAAT TGGCCTGCCG 
GGGCCACCAG GAGTGCTCGG GCTCATTGGT GACACAGGAG CACTGGGCCC AGTTGGCTAC 
CCAGGACCGA AAGGCATGAA GGGACTGATG GGCGGCGTGG GGGAGCCCGG ACTGAAAGGT 
GATAAGGGTG AACAAGGGGT GCCAGGTGTG TCGGGAGATC CTGGCTTCCA AGGAGACAAG 
GGGAGTCATG GCTTGCCAGG GCTCCCGGGT GGCCGGGGGA AGCCAGGGCC TCTGGGAAAA 
GCTGGAGACA AAGGATCACT TGGGTTTCCT GGACCTCCTG GACCTGAGGG TTTCCCAGGA 
GACATTGGCC CTCCAGGGGA CAATGGCCCT GAAGGCATGA AGGGTAAGCC TGGAGCCCGA 
GGCCTGCCTG GACCTCCTGG GCAGCTGGGG CCTGAGGGAG ATGAAGGACC CATGGGGCCA 
CCAGGAGTCC CAGGCTTGGA GGGTCAGCCT GGAAGGAAAG GGTTTCCTGG GAGGCCTGGC 
CTGGATGGCT CGAAGGGGGA ACCAGGGGAT CCTGGACGGC CAGGACCTGT GGGTGAACAG 
GGGCTTATGG GATTCATTGG TCTTGTCGGA GAGCCTGGAA TTGTTGGGGA GAAGGGAGAC 
CGTGGGGTGA TGGGACCCCC AGGTGCACCT GGACCCAAGG GGTCGATGGG CCATCCTGGA 
ACACCCGGTG GTATTGGGAA CCCTGGAGAG CCTGGACCCT GGGGCCCTCC AGGATCTCGA 
GGCCTGCCAG GCATGAGGGG AGCCAAGGGG CACCGGGGCC CTCGAGGACC TGATGGACCA 
GCTGGGGAGC AGGGATCCAA GGGCCTGAAG GGTCGTGTAG GACCTCGGGG CAGACCTGGC 
CAGCCAGGAC AGCAGGGTGC AGCTGGTGAG CGAGGCCACT CAGGTGCAAA AGGCTTCCTT 
GGCATCCCTG GTCCCTCAGG CCCTCCAGGT GCCAAGGGCC TCCCAGGAGA ACCGGGCTCT 
CAGGGACCTC AGGGGCCAGT TGGTCCTCCA GGAGAAATGG GGCCCAAGGG ACCGCCGGGT 
GCAGTGGGAG AGCCTGGCCT TCCTGGGGAC TCCGGAATGA AGGGTGACCT CGGACCTCTG 
GGCCCCCCTG GGGAGCAAGG TCTTATTGGG CAGCGGGGAG AGCCAGGCCT TGAGGGAGAC 
CATGGCCCTG TGGGGCCAGA TGGGCTGAAG GGCGACAGGG GCGACCCAGG GCCCGATGGC 
GAGCATGGTG AAAAGGGCCA GGAAGGGCTG AAAGGAGAGG ATGGGTCCCC TGGGCCCCCG 
GGCATCACTG GCGTCCCGGG TCGTGAGGGG AAGCCGGGCA AGCAGGGTGA GAAAGGCCAG 
AGGGGAGCTA AGGGTGCCAA GGGCCACCAA GGATATCTGG GAGAGATGGG CATCCCCGGA 
GAACCTGGGC CCCCTGGGAC CCCAGGTCCT AAAGGGTCCC GGGGCACCCT AGGACCAACG 
GGTGCTCCAG GACGGATGGG AGCCCAAGGA GAACCAGGAT TGGCCGGTTA TAATGGACAC 
AAAGGCATCA CAGGACCCCT CGGGCCTCCC GGGCCCAAAG GCGAGAAGGG GGACCAGGGC 
GAGGATGGCA AGACCGAGGG GCCCCCTGGG CCACCAGGAG ATCGGGGTCC TGTGGGTGAT 
CGAGGAGACC GTGGGGAGCC AGGCGACCCT GGATACCCTG GTCAAGAGGG AGTTCAAGGC 
CTCCGTGGAG AACCGGGCCA GCAGGGACAG CCTGGGCATC CAGGACCCCG GGGGCGCCCA 
GGACCCAAAG GATCAAAAGG CGAAGAGGGC CCAAAGGGAA AGCCAGGCAA GGCTGGGCCA 
TCAGGCCGGA GGGGGACCCA GGTGAGTTGC CATAGCTTCT CTGTCACCTT CTATGTTCTC 
CTGAGAAGGT TTAACCAGCT TCTTCTGTAC CTCCAGGGCC TTCAAGGACT GCCAGGCCCC 
CGAGGCGTGG TGGGGAGACA GGGCCCTGAG GGCACTGCTG GATCTGATGG GATTCCTGGC 
AGAGATGGTC GGCCAGGATA TCAGGGAGAC CAGGGAAATG ATGGGGACCC TGGCCCTGTG 
GGCCCTGCTG GGAGAAGAGG AAATCCAGGT GTGGCTGGCT TGCCTGGAGC ACAGGGGCCT 
CCGGGATTCA AGGGTGAAAG TGGGTTACCT GGGCAACTGG GTCCCCCTGG AAAACGAGGG 
ACAGAAGGTG GAACAGGGCT TCCTGGGAAC CAGGGGGAGC CAGGATCCAA AGGCCAGCCG 
GGTGACTCTG GCGAGATGGG CTTCCCAGGA GTGGCTGGCC TCTTTGGACC CAAGGGCCCC 
CCTGGAGACA TTGGCTTCAA AGGCATCCAA GGTCCTCGGG GGCCTCCTGG CTTGATGGGA 
AAGGAAGGTA TCATTGGCCC CCCCGGAATG CTGGGACCTT CTGGACTCCC GGGTCCCAAA 
GGTGACAGAG GCAGCCGAGG AGACTTGGGA CTGCAAGGCC CAAGGGGTCC TCCTGGTCCA 
AGGGGCCGGC CAGGTCCCCC GGGCCCTCCT TGGCATCCCA TCCAGTTTCA GCAAGATGAC 
CTTGGAGCAG CTTTCCAGAC ATGGATGGAT GCTCAAGGAG CCGTCAGATC GGAGCAGGGG 
TACAGCTATC CGGACCAGCT GGCGCTAGAC CAGGGAGGGG AGATCTTCAA AACCTTACAC 
TACCTCAGCA ACCTCATCCA GAGCATTAAG ACACCCTTGG GCACCAAGGA GAACCCAGCC 
CGGGTCTGCC GGGACCTCAT GGACTGTGAG CAGAGGATGG CGGATGGTAC CTACTGGGTG 
GACCCCAACC TCGGCTGCTC CTCTGACACC ATTGAAGTCT CCTGCAACTT TACACAGGGT 
GGGCAGACGT GCCTGAAGCC CATCACGGCC TCCAAGGCCG AGTTTGCTGT GAGTCGGGTC 
CAGATGAATT TCTTGCACCT GCTGAGCTCT GAGGGGACAC AGCATATCAC AATCCACTGT 
CTGAACATGA CGGTGTGGCA GGAGGGACCG GGACGCTCCT CTGCCAGACA GGCTGTGCGC 
TTCCGTGCCT GGAACGGACA GGTCTTCGAA GCTGGGGGTC AGTTCAGGCC AGAGGTGTCT 
ATGGATGGCT GCAAGGTCCA TGATGGCCGC TGGCATCAGA CACTGTTCAC CTTCCGGACC 
CAGGACCCCC AGCAGCTGCC CATCGTCAGT GTGGACAATC TCCCGCCTGT CTCATCAGGG 
AAGCAGTACC GCCTGGAAGT TGGACCTGCG TGCTTCCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col27a1 ( XM_030253632.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030109492.1 Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OMu88567
Clone ID Related Accession (Same CDS sequence)	XM_030253632.1
Accession Version	XM_030253632.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3267bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	collagen alpha-1(XXVII) chain isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGCG GGGTCTCCCT GGCGTTCCTG GCAAGAGGGG CGAGATGGGG CTTCCCTGGA 
GACTTTGGGG AAAGAGGCCC CCCTGGACTT GATGGCAACC CTGGAGAAAT TGGCCTGCCG 
GGGCCACCAG GAGTGCTCGG GCTCATTGGT GACACAGGAG CACTGGGCCC AGTTGGCTAC 
CCAGGACCGA AAGGCATGAA GGGACTGATG GGCGGCGTGG GGGAGCCCGG ACTGAAAGGT 
GATAAGGGTG AACAAGGGGT GCCAGGTGTG TCGGGAGATC CTGGCTTCCA AGGAGACAAG 
GGGAGTCATG GCTTGCCAGG GCTCCCGGGT GGCCGGGGGA AGCCAGGGCC TCTGGGAAAA 
GCTGGAGACA AAGGATCACT TGGGTTTCCT GGACCTCCTG GACCTGAGGG TTTCCCAGGA 
GACATTGGCC CTCCAGGGGA CAATGGCCCT GAAGGCATGA AGGGTAAGCC TGGAGCCCGA 
GGCCTGCCTG GACCTCCTGG GCAGCTGGGG CCTGAGGGAG ATGAAGGACC CATGGGGCCA 
CCAGGAGTCC CAGGCTTGGA GGGTCAGCCT GGAAGGAAAG GGTTTCCTGG GAGGCCTGGC 
CTGGATGGCT CGAAGGGGGA ACCAGGGGAT CCTGGACGGC CAGGACCTGT GGGTGAACAG 
GGGCTTATGG GATTCATTGG TCTTGTCGGA GAGCCTGGAA TTGTTGGGGA GAAGGGAGAC 
CGTGGGGTGA TGGGACCCCC AGGTGCACCT GGACCCAAGG GGTCGATGGG CCATCCTGGA 
ACACCCGGTG GTATTGGGAA CCCTGGAGAG CCTGGACCCT GGGGCCCTCC AGGATCTCGA 
GGCCTGCCAG GCATGAGGGG AGCCAAGGGG CACCGGGGCC CTCGAGGACC TGATGGACCA 
GCTGGGGAGC AGGGATCCAA GGGCCTGAAG GGTCGTGTAG GACCTCGGGG CAGACCTGGC 
CAGCCAGGAC AGCAGGGTGC AGCTGGTGAG CGAGGCCACT CAGGTGCAAA AGGCTTCCTT 
GGCATCCCTG GTCCCTCAGG CCCTCCAGGT GCCAAGGGCC TCCCAGGAGA ACCGGGCTCT 
CAGGGACCTC AGGGGCCAGT TGGTCCTCCA GGAGAAATGG GGCCCAAGGG ACCGCCGGGT 
GCAGTGGGAG AGCCTGGCCT TCCTGGGGAC TCCGGAATGA AGGGTGACCT CGGACCTCTG 
GGCCCCCCTG GGGAGCAAGG TCTTATTGGG CAGCGGGGAG AGCCAGGCCT TGAGGGAGAC 
CATGGCCCTG TGGGGCCAGA TGGGCTGAAG GGCGACAGGG GCGACCCAGG GCCCGATGGC 
GAGCATGGTG AAAAGGGCCA GGAAGGGCTG AAAGGAGAGG ATGGGTCCCC TGGGCCCCCG 
GGCATCACTG GCGTCCCGGG TCGTGAGGGG AAGCCGGGCA AGCAGGGTGA GAAAGGCCAG 
AGGGGAGCTA AGGGTGCCAA GGGCCACCAA GGATATCTGG GAGAGATGGG CATCCCCGGA 
GAACCTGGGC CCCCTGGGAC CCCAGGTCCT AAAGGGTCCC GGGGCACCCT AGGACCAACG 
GGTGCTCCAG GACGGATGGG AGCCCAAGGA GAACCAGGAT TGGCCGGTTA TAATGGACAC 
AAAGGCATCA CAGGACCCCT CGGGCCTCCC GGGCCCAAAG GCGAGAAGGG GGACCAGGGC 
GAGGATGGCA AGACCGAGGG GCCCCCTGGG CCACCAGGAG ATCGGGGTCC TGTGGGTGAT 
CGAGGAGACC GTGGGGAGCC AGGCGACCCT GGATACCCTG GTCAAGAGGG AGTTCAAGGC 
CTCCGTGGAG AACCGGGCCA GCAGGGACAG CCTGGGCATC CAGGACCCCG GGGGCGCCCA 
GGACCCAAAG GATCAAAAGG CGAAGAGGGC CCAAAGGGAA AGCCAGGCAA GGCTGGGCCA 
TCAGGCCGGA GGGGGACCCA GGGCCTTCAA GGACTGCCAG GCCCCCGAGG CGTGGTGGGG 
AGACAGGGCC CTGAGGGCAC TGCTGGATCT GATGGGATTC CTGGCAGAGA TGGTCGGCCA 
GGATATCAGG GAGACCAGGG AAATGATGGG GACCCTGGCC CTGTGGGCCC TGCTGGGAGA 
AGAGGAAATC CAGGTGTGGC TGGCTTGCCT GGAGCACAGG GGCCTCCGGG ATTCAAGGGT 
GAAAGTGGGT TACCTGGGCA ACTGGGTCCC CCTGGAAAAC GAGGGACAGA AGGTGGAACA 
GGGCTTCCTG GGAACCAGGG GGAGCCAGGA TCCAAAGGCC AGCCGGGTGA CTCTGGCGAG 
ATGGGCTTCC CAGGAGTGGC TGGCCTCTTT GGACCCAAGG GCCCCCCTGG AGACATTGGC 
TTCAAAGGCA TCCAAGGTCC TCGGGGGCCT CCTGGCTTGA TGGGAAAGGA AGGTATCATT 
GGCCCCCCCG GAATGCTGGG ACCTTCTGGA CTCCCGGGTC CCAAAGGTGA CAGAGGCAGC 
CGAGGAGACT TGGGACTGCA AGGCCCAAGG GGTCCTCCTG GTCCAAGGGG CCGGCCAGGT 
CCCCCGGGCC CTCCTTGGCA TCCCATCCAG TTTCAGCAAG ATGACCTTGG AGCAGCTTTC 
CAGACATGGA TGGATGCTCA AGGAGCCGTC AGATCGGAGC AGGGGTACAG CTATCCGGAC 
CAGCTGGCGC TAGACCAGGG AGGGGAGATC TTCAAAACCT TACACTACCT CAGCAACCTC 
ATCCAGAGCA TTAAGACACC CTTGGGCACC AAGGAGAACC CAGCCCGGGT CTGCCGGGAC 
CTCATGGACT GTGAGCAGAG GATGGCGGAT GGTACCTACT GGGTGGACCC CAACCTCGGC 
TGCTCCTCTG ACACCATTGA AGTCTCCTGC AACTTTACAC AGGGTGGGCA GACGTGCCTG 
AAGCCCATCA CGGCCTCCAA GGCCGAGTTT GCTGTGAGTC GGGTCCAGAT GAATTTCTTG 
CACCTGCTGA GCTCTGAGGG GACACAGCAT ATCACAATCC ACTGTCTGAA CATGACGGTG 
TGGCAGGAGG GACCGGGACG CTCCTCTGCC AGACAGGCTG TGCGCTTCCG TGCCTGGAAC 
GGACAGGTCT TCGAAGCTGG GGGTCAGTTC AGGCCAGAGG TGTCTATGGA TGGCTGCAAG 
GTCCATGATG GCCGCTGGCA TCAGACACTG TTCACCTTCC GGACCCAGGA CCCCCAGCAG 
CTGCCCATCG TCAGTGTGGA CAATCTCCCG CCTGTCTCAT CAGGGAAGCA GTACCGCCTG 
GAAGTTGGAC CTGCGTGCTT CCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030109492.1
CDS	90..3356
Translation	MESGVSLAFLARGARWGFPGDFGERGPPGLDGNPGEIGLPGPPGVLGLIGDTGALGPVGYPGPKGMKGLMGGVGEPGLKGDKGEQGVPGVSGDPGFQGDKGSHGLPGLPGGRGKPGPLGKAGDKGSLGFPGPPGPEGFPGDIGPPGDNGPEGMKGKPGARGLPGPPGQLGPEGDEGPMGPPGVPGLEGQPGRKGFPGRPGLDGSKGEPGDPGRPGPVGEQGLMGFIGLVGEPGIVGEKGDRGVMGPPGAPGPKGSMGHPGTPGGIGNPGEPGPWGPPGSRGLPGMRGAKGHRGPRGPDGPAGEQGSKGLKGRVGPRGRPGQPGQQGAAGERGHSGAKGFLGIPGPSGPPGAKGLPGEPGSQGPQGPVGPPGEMGPKGPPGAVGEPGLPGDSGMKGDLGPLGPPGEQGLIGQRGEPGLEGDHGPVGPDGLKGDRGDPGPDGEHGEKGQEGLKGEDGSPGPPGITGVPGREGKPGKQGEKGQRGAKGAKGHQGYLGEMGIPGEPGPPGTPGPKGSRGTLGPTGAPGRMGAQGEPGLAGYNGHKGITGPLGPPGPKGEKGDQGEDGKTEGPPGPPGDRGPVGDRGDRGEPGDPGYPGQEGVQGLRGEPGQQGQPGHPGPRGRPGPKGSKGEEGPKGKPGKAGPSGRRGTQGLQGLPGPRGVVGRQGPEGTAGSDGIPGRDGRPGYQGDQGNDGDPGPVGPAGRRGNPGVAGLPGAQGPPGFKGESGLPGQLGPPGKRGTEGGTGLPGNQGEPGSKGQPGDSGEMGFPGVAGLFGPKGPPGDIGFKGIQGPRGPPGLMGKEGIIGPPGMLGPSGLPGPKGDRGSRGDLGLQGPRGPPGPRGRPGPPGPPWHPIQFQQDDLGAAFQTWMDAQGAVRSEQGYSYPDQLALDQGGEIFKTLHYLSNLIQSIKTPLGTKENPARVCRDLMDCEQRMADGTYWVDPNLGCSSDTIEVSCNFTQGGQTCLKPITASKAEFAVSRVQMNFLHLLSSEGTQHITIHCLNMTVWQEGPGRSSARQAVRFRAWNGQVFEAGGQFRPEVSMDGCKVHDGRWHQTLFTFRTQDPQQLPIVSVDNLPPVSSGKQYRLEVGPACFL

Target ORF information:

RefSeq Version	XM_030253632.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030253632.1

ORF Insert Sequence:

ATGGAGAGCG GGGTCTCCCT GGCGTTCCTG GCAAGAGGGG CGAGATGGGG CTTCCCTGGA 
GACTTTGGGG AAAGAGGCCC CCCTGGACTT GATGGCAACC CTGGAGAAAT TGGCCTGCCG 
GGGCCACCAG GAGTGCTCGG GCTCATTGGT GACACAGGAG CACTGGGCCC AGTTGGCTAC 
CCAGGACCGA AAGGCATGAA GGGACTGATG GGCGGCGTGG GGGAGCCCGG ACTGAAAGGT 
GATAAGGGTG AACAAGGGGT GCCAGGTGTG TCGGGAGATC CTGGCTTCCA AGGAGACAAG 
GGGAGTCATG GCTTGCCAGG GCTCCCGGGT GGCCGGGGGA AGCCAGGGCC TCTGGGAAAA 
GCTGGAGACA AAGGATCACT TGGGTTTCCT GGACCTCCTG GACCTGAGGG TTTCCCAGGA 
GACATTGGCC CTCCAGGGGA CAATGGCCCT GAAGGCATGA AGGGTAAGCC TGGAGCCCGA 
GGCCTGCCTG GACCTCCTGG GCAGCTGGGG CCTGAGGGAG ATGAAGGACC CATGGGGCCA 
CCAGGAGTCC CAGGCTTGGA GGGTCAGCCT GGAAGGAAAG GGTTTCCTGG GAGGCCTGGC 
CTGGATGGCT CGAAGGGGGA ACCAGGGGAT CCTGGACGGC CAGGACCTGT GGGTGAACAG 
GGGCTTATGG GATTCATTGG TCTTGTCGGA GAGCCTGGAA TTGTTGGGGA GAAGGGAGAC 
CGTGGGGTGA TGGGACCCCC AGGTGCACCT GGACCCAAGG GGTCGATGGG CCATCCTGGA 
ACACCCGGTG GTATTGGGAA CCCTGGAGAG CCTGGACCCT GGGGCCCTCC AGGATCTCGA 
GGCCTGCCAG GCATGAGGGG AGCCAAGGGG CACCGGGGCC CTCGAGGACC TGATGGACCA 
GCTGGGGAGC AGGGATCCAA GGGCCTGAAG GGTCGTGTAG GACCTCGGGG CAGACCTGGC 
CAGCCAGGAC AGCAGGGTGC AGCTGGTGAG CGAGGCCACT CAGGTGCAAA AGGCTTCCTT 
GGCATCCCTG GTCCCTCAGG CCCTCCAGGT GCCAAGGGCC TCCCAGGAGA ACCGGGCTCT 
CAGGGACCTC AGGGGCCAGT TGGTCCTCCA GGAGAAATGG GGCCCAAGGG ACCGCCGGGT 
GCAGTGGGAG AGCCTGGCCT TCCTGGGGAC TCCGGAATGA AGGGTGACCT CGGACCTCTG 
GGCCCCCCTG GGGAGCAAGG TCTTATTGGG CAGCGGGGAG AGCCAGGCCT TGAGGGAGAC 
CATGGCCCTG TGGGGCCAGA TGGGCTGAAG GGCGACAGGG GCGACCCAGG GCCCGATGGC 
GAGCATGGTG AAAAGGGCCA GGAAGGGCTG AAAGGAGAGG ATGGGTCCCC TGGGCCCCCG 
GGCATCACTG GCGTCCCGGG TCGTGAGGGG AAGCCGGGCA AGCAGGGTGA GAAAGGCCAG 
AGGGGAGCTA AGGGTGCCAA GGGCCACCAA GGATATCTGG GAGAGATGGG CATCCCCGGA 
GAACCTGGGC CCCCTGGGAC CCCAGGTCCT AAAGGGTCCC GGGGCACCCT AGGACCAACG 
GGTGCTCCAG GACGGATGGG AGCCCAAGGA GAACCAGGAT TGGCCGGTTA TAATGGACAC 
AAAGGCATCA CAGGACCCCT CGGGCCTCCC GGGCCCAAAG GCGAGAAGGG GGACCAGGGC 
GAGGATGGCA AGACCGAGGG GCCCCCTGGG CCACCAGGAG ATCGGGGTCC TGTGGGTGAT 
CGAGGAGACC GTGGGGAGCC AGGCGACCCT GGATACCCTG GTCAAGAGGG AGTTCAAGGC 
CTCCGTGGAG AACCGGGCCA GCAGGGACAG CCTGGGCATC CAGGACCCCG GGGGCGCCCA 
GGACCCAAAG GATCAAAAGG CGAAGAGGGC CCAAAGGGAA AGCCAGGCAA GGCTGGGCCA 
TCAGGCCGGA GGGGGACCCA GGGCCTTCAA GGACTGCCAG GCCCCCGAGG CGTGGTGGGG 
AGACAGGGCC CTGAGGGCAC TGCTGGATCT GATGGGATTC CTGGCAGAGA TGGTCGGCCA 
GGATATCAGG GAGACCAGGG AAATGATGGG GACCCTGGCC CTGTGGGCCC TGCTGGGAGA 
AGAGGAAATC CAGGTGTGGC TGGCTTGCCT GGAGCACAGG GGCCTCCGGG ATTCAAGGGT 
GAAAGTGGGT TACCTGGGCA ACTGGGTCCC CCTGGAAAAC GAGGGACAGA AGGTGGAACA 
GGGCTTCCTG GGAACCAGGG GGAGCCAGGA TCCAAAGGCC AGCCGGGTGA CTCTGGCGAG 
ATGGGCTTCC CAGGAGTGGC TGGCCTCTTT GGACCCAAGG GCCCCCCTGG AGACATTGGC 
TTCAAAGGCA TCCAAGGTCC TCGGGGGCCT CCTGGCTTGA TGGGAAAGGA AGGTATCATT 
GGCCCCCCCG GAATGCTGGG ACCTTCTGGA CTCCCGGGTC CCAAAGGTGA CAGAGGCAGC 
CGAGGAGACT TGGGACTGCA AGGCCCAAGG GGTCCTCCTG GTCCAAGGGG CCGGCCAGGT 
CCCCCGGGCC CTCCTTGGCA TCCCATCCAG TTTCAGCAAG ATGACCTTGG AGCAGCTTTC 
CAGACATGGA TGGATGCTCA AGGAGCCGTC AGATCGGAGC AGGGGTACAG CTATCCGGAC 
CAGCTGGCGC TAGACCAGGG AGGGGAGATC TTCAAAACCT TACACTACCT CAGCAACCTC 
ATCCAGAGCA TTAAGACACC CTTGGGCACC AAGGAGAACC CAGCCCGGGT CTGCCGGGAC 
CTCATGGACT GTGAGCAGAG GATGGCGGAT GGTACCTACT GGGTGGACCC CAACCTCGGC 
TGCTCCTCTG ACACCATTGA AGTCTCCTGC AACTTTACAC AGGGTGGGCA GACGTGCCTG 
AAGCCCATCA CGGCCTCCAA GGCCGAGTTT GCTGTGAGTC GGGTCCAGAT GAATTTCTTG 
CACCTGCTGA GCTCTGAGGG GACACAGCAT ATCACAATCC ACTGTCTGAA CATGACGGTG 
TGGCAGGAGG GACCGGGACG CTCCTCTGCC AGACAGGCTG TGCGCTTCCG TGCCTGGAAC 
GGACAGGTCT TCGAAGCTGG GGGTCAGTTC AGGCCAGAGG TGTCTATGGA TGGCTGCAAG 
GTCCATGATG GCCGCTGGCA TCAGACACTG TTCACCTTCC GGACCCAGGA CCCCCAGCAG 
CTGCCCATCG TCAGTGTGGA CAATCTCCCG CCTGTCTCAT CAGGGAAGCA GTACCGCCTG 
GAAGTTGGAC CTGCGTGCTT CCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col27a1 ( XM_030253633.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030109493.1 Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X8, mRNA.

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CloneID	OMu88568
Clone ID Related Accession (Same CDS sequence)	XM_030253633.1
Accession Version	XM_030253633.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	collagen alpha-1(XXVII) chain isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTAGGC CGGGCTTCCC TGGAGACTTT GGGGAAAGAG GCCCCCCTGG ACTTGATGGC 
AACCCTGGAG AAATTGGCCT GCCGGGGCCA CCAGGAGTGC TCGGGCTCAT TGGTGACACA 
GGAGCACTGG GCCCAGTTGG CTACCCAGGA CCGAAAGGCA TGAAGGGACT GATGGGCGGC 
GTGGGGGAGC CCGGACTGAA AGGTGATAAG GGTGAACAAG GGGTGCCAGG TGTGTCGGGA 
GATCCTGGCT TCCAAGGAGA CAAGGGGAGT CATGGCTTGC CAGGGCTCCC GGGTGGCCGG 
GGGAAGCCAG GGCCTCTGGG AAAAGCTGGA GACAAAGGAT CACTTGGGTT TCCTGGACCT 
CCTGGACCTG AGGGTTTCCC AGGAGACATT GGCCCTCCAG GGGACAATGG CCCTGAAGGC 
ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG CCTGGACCTC CTGGGCAGCT GGGGCCTGAG 
GGAGATGAAG GACCCATGGG GCCACCAGGA GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG 
AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT GGCTCGAAGG GGGAACCAGG GGATCCTGGA 
CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT ATGGGATTCA TTGGTCTTGT CGGAGAGCCT 
GGAATTGTTG GGGAGAAGGG AGACCGTGGG GTGATGGGAC CCCCAGGTGC ACCTGGACCC 
AAGGGGTCGA TGGGCCATCC TGGAACACCC GGTGGTATTG GGAACCCTGG AGAGCCTGGA 
CCCTGGGGCC CTCCAGGATC TCGAGGCCTG CCAGGCATGA GGGGAGCCAA GGGGCACCGG 
GGCCCTCGAG GACCTGATGG ACCAGCTGGG GAGCAGGGAT CCAAGGGCCT GAAGGGTCGT 
GTAGGACCTC GGGGCAGACC TGGCCAGCCA GGACAGCAGG GTGCAGCTGG TGAGCGAGGC 
CACTCAGGTG CAAAAGGCTT CCTTGGCATC CCTGGTCCCT CAGGCCCTCC AGGTGCCAAG 
GGCCTCCCAG GAGAACCGGG CTCTCAGGGA CCTCAGGGGC CAGTTGGTCC TCCAGGAGAA 
ATGGGGCCCA AGGGACCGCC GGGTGCAGTG GGAGAGCCTG GCCTTCCTGG GGACTCCGGA 
ATGAAGGGTG ACCTCGGACC TCTGGGCCCC CCTGGGGAGC AAGGTCTTAT TGGGCAGCGG 
GGAGAGCCAG GCCTTGAGGG AGACCATGGC CCTGTGGGGC CAGATGGGCT GAAGGGCGAC 
AGGGGCGACC CAGGGCCCGA TGGCGAGCAT GGTGAAAAGG GCCAGGAAGG GCTGAAAGGA 
GAGGATGGGT CCCCTGGGCC CCCGGGCATC ACTGGCGTCC CGGGTCGTGA GGGGAAGCCG 
GGCAAGCAGG GTGAGAAAGG CCAGAGGGGA GCTAAGGGTG CCAAGGGCCA CCAAGGATAT 
CTGGGAGAGA TGGGCATCCC CGGAGAACCT GGGCCCCCTG GGACCCCAGG TCCTAAAGGG 
TCCCGGGGCA CCCTAGGACC AACGGGTGCT CCAGGACGGA TGGGAGCCCA AGGAGAACCA 
GGATTGGCCG GTTATAATGG ACACAAAGGC ATCACAGGAC CCCTCGGGCC TCCCGGGCCC 
AAAGGCGAGA AGGGGGACCA GGGCGAGGAT GGCAAGACCG AGGGGCCCCC TGGGCCACCA 
GGAGATCGGG GTCCTGTGGG TGATCGAGGA GACCGTGGGG AGCCAGGCGA CCCTGGATAC 
CCTGGTCAAG AGGGAGTTCA AGGCCTCCGT GGAGAACCGG GCCAGCAGGG ACAGCCTGGG 
CATCCAGGAC CCCGGGGGCG CCCAGGACCC AAAGGATCAA AAGGCGAAGA GGGCCCAAAG 
GGAAAGCCAG GCAAGGCTGG GCCATCAGGC CGGAGGGGGA CCCAGGGCCT TCAAGGACTG 
CCAGGCCCCC GAGGCGTGGT GGGGAGACAG GGCCCTGAGG GCACTGCTGG ATCTGATGGG 
ATTCCTGGCA GAGATGGTCG GCCAGGATAT CAGGGAGACC AGGGAAATGA TGGGGACCCT 
GGCCCTGTGG GCCCTGCTGG GAGAAGAGGA AATCCAGGTG TGGCTGGCTT GCCTGGAGCA 
CAGGGGCCTC CGGGATTCAA GGGTGAAAGT GGGTTACCTG GGCAACTGGG TCCCCCTGGA 
AAACGAGGGA CAGAAGGTGG AACAGGGCTT CCTGGGAACC AGGGGGAGCC AGGATCCAAA 
GGCCAGCCGG GTGACTCTGG CGAGATGGGC TTCCCAGGAG TGGCTGGCCT CTTTGGACCC 
AAGGGCCCCC CTGGAGACAT TGGCTTCAAA GGCATCCAAG GTCCTCGGGG GCCTCCTGGC 
TTGATGGGAA AGGAAGGTAT CATTGGCCCC CCCGGAATGC TGGGACCTTC TGGACTCCCG 
GGTCCCAAAG GTGACAGAGG CAGCCGAGGA GACTTGGGAC TGCAAGGCCC AAGGGGTCCT 
CCTGGTCCAA GGGGCCGGCC AGGTCCCCCG GGCCCTCCTT GGCATCCCAT CCAGTTTCAG 
CAAGATGACC TTGGAGCAGC TTTCCAGACA TGGATGGATG CTCAAGGAGC CGTCAGATCG 
GAGCAGGGGT ACAGCTATCC GGACCAGCTG GCGCTAGACC AGGGAGGGGA GATCTTCAAA 
ACCTTACACT ACCTCAGCAA CCTCATCCAG AGCATTAAGA CACCCTTGGG CACCAAGGAG 
AACCCAGCCC GGGTCTGCCG GGACCTCATG GACTGTGAGC AGAGGATGGC GGATGGTACC 
TACTGGGTGG ACCCCAACCT CGGCTGCTCC TCTGACACCA TTGAAGTCTC CTGCAACTTT 
ACACAGGGTG GGCAGACGTG CCTGAAGCCC ATCACGGCCT CCAAGGCCGA GTTTGCTGTG 
AGTCGGGTCC AGATGAATTT CTTGCACCTG CTGAGCTCTG AGGGGACACA GCATATCACA 
ATCCACTGTC TGAACATGAC GGTGTGGCAG GAGGGACCGG GACGCTCCTC TGCCAGACAG 
GCTGTGCGCT TCCGTGCCTG GAACGGACAG GTCTTCGAAG CTGGGGGTCA GTTCAGGCCA 
GAGGTGTCTA TGGATGGCTG CAAGGTCCAT GATGGCCGCT GGCATCAGAC ACTGTTCACC 
TTCCGGACCC AGGACCCCCA GCAGCTGCCC ATCGTCAGTG TGGACAATCT CCCGCCTGTC 
TCATCAGGGA AGCAGTACCG CCTGGAAGTT GGACCTGCGT GCTTCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030109493.1
CDS	2811..6041
Translation	MGRPGFPGDFGERGPPGLDGNPGEIGLPGPPGVLGLIGDTGALGPVGYPGPKGMKGLMGGVGEPGLKGDKGEQGVPGVSGDPGFQGDKGSHGLPGLPGGRGKPGPLGKAGDKGSLGFPGPPGPEGFPGDIGPPGDNGPEGMKGKPGARGLPGPPGQLGPEGDEGPMGPPGVPGLEGQPGRKGFPGRPGLDGSKGEPGDPGRPGPVGEQGLMGFIGLVGEPGIVGEKGDRGVMGPPGAPGPKGSMGHPGTPGGIGNPGEPGPWGPPGSRGLPGMRGAKGHRGPRGPDGPAGEQGSKGLKGRVGPRGRPGQPGQQGAAGERGHSGAKGFLGIPGPSGPPGAKGLPGEPGSQGPQGPVGPPGEMGPKGPPGAVGEPGLPGDSGMKGDLGPLGPPGEQGLIGQRGEPGLEGDHGPVGPDGLKGDRGDPGPDGEHGEKGQEGLKGEDGSPGPPGITGVPGREGKPGKQGEKGQRGAKGAKGHQGYLGEMGIPGEPGPPGTPGPKGSRGTLGPTGAPGRMGAQGEPGLAGYNGHKGITGPLGPPGPKGEKGDQGEDGKTEGPPGPPGDRGPVGDRGDRGEPGDPGYPGQEGVQGLRGEPGQQGQPGHPGPRGRPGPKGSKGEEGPKGKPGKAGPSGRRGTQGLQGLPGPRGVVGRQGPEGTAGSDGIPGRDGRPGYQGDQGNDGDPGPVGPAGRRGNPGVAGLPGAQGPPGFKGESGLPGQLGPPGKRGTEGGTGLPGNQGEPGSKGQPGDSGEMGFPGVAGLFGPKGPPGDIGFKGIQGPRGPPGLMGKEGIIGPPGMLGPSGLPGPKGDRGSRGDLGLQGPRGPPGPRGRPGPPGPPWHPIQFQQDDLGAAFQTWMDAQGAVRSEQGYSYPDQLALDQGGEIFKTLHYLSNLIQSIKTPLGTKENPARVCRDLMDCEQRMADGTYWVDPNLGCSSDTIEVSCNFTQGGQTCLKPITASKAEFAVSRVQMNFLHLLSSEGTQHITIHCLNMTVWQEGPGRSSARQAVRFRAWNGQVFEAGGQFRPEVSMDGCKVHDGRWHQTLFTFRTQDPQQLPIVSVDNLPPVSSGKQYRLEVGPACFL

Target ORF information:

RefSeq Version	XM_030253633.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030253633.1

ORF Insert Sequence:

ATGGGTAGGC CGGGCTTCCC TGGAGACTTT GGGGAAAGAG GCCCCCCTGG ACTTGATGGC 
AACCCTGGAG AAATTGGCCT GCCGGGGCCA CCAGGAGTGC TCGGGCTCAT TGGTGACACA 
GGAGCACTGG GCCCAGTTGG CTACCCAGGA CCGAAAGGCA TGAAGGGACT GATGGGCGGC 
GTGGGGGAGC CCGGACTGAA AGGTGATAAG GGTGAACAAG GGGTGCCAGG TGTGTCGGGA 
GATCCTGGCT TCCAAGGAGA CAAGGGGAGT CATGGCTTGC CAGGGCTCCC GGGTGGCCGG 
GGGAAGCCAG GGCCTCTGGG AAAAGCTGGA GACAAAGGAT CACTTGGGTT TCCTGGACCT 
CCTGGACCTG AGGGTTTCCC AGGAGACATT GGCCCTCCAG GGGACAATGG CCCTGAAGGC 
ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG CCTGGACCTC CTGGGCAGCT GGGGCCTGAG 
GGAGATGAAG GACCCATGGG GCCACCAGGA GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG 
AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT GGCTCGAAGG GGGAACCAGG GGATCCTGGA 
CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT ATGGGATTCA TTGGTCTTGT CGGAGAGCCT 
GGAATTGTTG GGGAGAAGGG AGACCGTGGG GTGATGGGAC CCCCAGGTGC ACCTGGACCC 
AAGGGGTCGA TGGGCCATCC TGGAACACCC GGTGGTATTG GGAACCCTGG AGAGCCTGGA 
CCCTGGGGCC CTCCAGGATC TCGAGGCCTG CCAGGCATGA GGGGAGCCAA GGGGCACCGG 
GGCCCTCGAG GACCTGATGG ACCAGCTGGG GAGCAGGGAT CCAAGGGCCT GAAGGGTCGT 
GTAGGACCTC GGGGCAGACC TGGCCAGCCA GGACAGCAGG GTGCAGCTGG TGAGCGAGGC 
CACTCAGGTG CAAAAGGCTT CCTTGGCATC CCTGGTCCCT CAGGCCCTCC AGGTGCCAAG 
GGCCTCCCAG GAGAACCGGG CTCTCAGGGA CCTCAGGGGC CAGTTGGTCC TCCAGGAGAA 
ATGGGGCCCA AGGGACCGCC GGGTGCAGTG GGAGAGCCTG GCCTTCCTGG GGACTCCGGA 
ATGAAGGGTG ACCTCGGACC TCTGGGCCCC CCTGGGGAGC AAGGTCTTAT TGGGCAGCGG 
GGAGAGCCAG GCCTTGAGGG AGACCATGGC CCTGTGGGGC CAGATGGGCT GAAGGGCGAC 
AGGGGCGACC CAGGGCCCGA TGGCGAGCAT GGTGAAAAGG GCCAGGAAGG GCTGAAAGGA 
GAGGATGGGT CCCCTGGGCC CCCGGGCATC ACTGGCGTCC CGGGTCGTGA GGGGAAGCCG 
GGCAAGCAGG GTGAGAAAGG CCAGAGGGGA GCTAAGGGTG CCAAGGGCCA CCAAGGATAT 
CTGGGAGAGA TGGGCATCCC CGGAGAACCT GGGCCCCCTG GGACCCCAGG TCCTAAAGGG 
TCCCGGGGCA CCCTAGGACC AACGGGTGCT CCAGGACGGA TGGGAGCCCA AGGAGAACCA 
GGATTGGCCG GTTATAATGG ACACAAAGGC ATCACAGGAC CCCTCGGGCC TCCCGGGCCC 
AAAGGCGAGA AGGGGGACCA GGGCGAGGAT GGCAAGACCG AGGGGCCCCC TGGGCCACCA 
GGAGATCGGG GTCCTGTGGG TGATCGAGGA GACCGTGGGG AGCCAGGCGA CCCTGGATAC 
CCTGGTCAAG AGGGAGTTCA AGGCCTCCGT GGAGAACCGG GCCAGCAGGG ACAGCCTGGG 
CATCCAGGAC CCCGGGGGCG CCCAGGACCC AAAGGATCAA AAGGCGAAGA GGGCCCAAAG 
GGAAAGCCAG GCAAGGCTGG GCCATCAGGC CGGAGGGGGA CCCAGGGCCT TCAAGGACTG 
CCAGGCCCCC GAGGCGTGGT GGGGAGACAG GGCCCTGAGG GCACTGCTGG ATCTGATGGG 
ATTCCTGGCA GAGATGGTCG GCCAGGATAT CAGGGAGACC AGGGAAATGA TGGGGACCCT 
GGCCCTGTGG GCCCTGCTGG GAGAAGAGGA AATCCAGGTG TGGCTGGCTT GCCTGGAGCA 
CAGGGGCCTC CGGGATTCAA GGGTGAAAGT GGGTTACCTG GGCAACTGGG TCCCCCTGGA 
AAACGAGGGA CAGAAGGTGG AACAGGGCTT CCTGGGAACC AGGGGGAGCC AGGATCCAAA 
GGCCAGCCGG GTGACTCTGG CGAGATGGGC TTCCCAGGAG TGGCTGGCCT CTTTGGACCC 
AAGGGCCCCC CTGGAGACAT TGGCTTCAAA GGCATCCAAG GTCCTCGGGG GCCTCCTGGC 
TTGATGGGAA AGGAAGGTAT CATTGGCCCC CCCGGAATGC TGGGACCTTC TGGACTCCCG 
GGTCCCAAAG GTGACAGAGG CAGCCGAGGA GACTTGGGAC TGCAAGGCCC AAGGGGTCCT 
CCTGGTCCAA GGGGCCGGCC AGGTCCCCCG GGCCCTCCTT GGCATCCCAT CCAGTTTCAG 
CAAGATGACC TTGGAGCAGC TTTCCAGACA TGGATGGATG CTCAAGGAGC CGTCAGATCG 
GAGCAGGGGT ACAGCTATCC GGACCAGCTG GCGCTAGACC AGGGAGGGGA GATCTTCAAA 
ACCTTACACT ACCTCAGCAA CCTCATCCAG AGCATTAAGA CACCCTTGGG CACCAAGGAG 
AACCCAGCCC GGGTCTGCCG GGACCTCATG GACTGTGAGC AGAGGATGGC GGATGGTACC 
TACTGGGTGG ACCCCAACCT CGGCTGCTCC TCTGACACCA TTGAAGTCTC CTGCAACTTT 
ACACAGGGTG GGCAGACGTG CCTGAAGCCC ATCACGGCCT CCAAGGCCGA GTTTGCTGTG 
AGTCGGGTCC AGATGAATTT CTTGCACCTG CTGAGCTCTG AGGGGACACA GCATATCACA 
ATCCACTGTC TGAACATGAC GGTGTGGCAG GAGGGACCGG GACGCTCCTC TGCCAGACAG 
GCTGTGCGCT TCCGTGCCTG GAACGGACAG GTCTTCGAAG CTGGGGGTCA GTTCAGGCCA 
GAGGTGTCTA TGGATGGCTG CAAGGTCCAT GATGGCCGCT GGCATCAGAC ACTGTTCACC 
TTCCGGACCC AGGACCCCCA GCAGCTGCCC ATCGTCAGTG TGGACAATCT CCCGCCTGTC 
TCATCAGGGA AGCAGTACCG CCTGGAAGTT GGACCTGCGT GCTTCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col27a1 ( XM_006538039.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006538102.1 Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OMu33009
Clone ID Related Accession (Same CDS sequence)	XM_006538039.3 , XM_006538039.4
Accession Version	XM_006538039.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3186bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	collagen alpha-1(XXVII) chain isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006538039.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCACGG GATTCGCGCG GGGGGCCCGA GGCACAGCGG CGTCAGGACC CGGGGGGGGG 
TTTCTCTTCG CCTGGATCTT GGTCTCATTT ACCTGTCACC TGGCCTCCAC CCAAGGAGCT 
CCTGAAGATG TGGATGTCCT CCAGCGGCTG GGCCTCAGCT GGACGAAGGC CGGGGGTGGC 
CGGAGTCCTA CACCCCCTGG TGTCATTCCT TTCCCATCTG GCTTCATCTT CACACAGCGG 
GCCAAGCTGC AGGCCCCCAC AGCCAATGTT CTTCCCACCA CCCTGGGGCG GGAGCTGGCA 
TTGGTGTTGA GCCTCTGTTC ACATCGTGTG AACCATGCCT TCCTCTTTGC CATCCGCAGC 
AGGAAACACA AGCTGCAACT GGGTCTGCAG TTCCTCCCCG GCAGGACGAT CATCCACCTG 
GGGCCTCGGC AATCAGTGGC CTTCGACTTG GATGTGCATG ATGGGCGCTG GCACCACCTG 
GCCCTAGAGT TGCGTGGCCG CACAGTCACA ATGGTGACAG CCTGTGGGCA GCACAGGGTA 
CCTGTCCCAC TGCCTTCCCG TAGGGACTCC ATGCTTGACC CCCAGGGCTC CTTTCTCTTG 
GGGAAGGTGA ACCCCCGTGC TGTCCAGTTT GAAGGTGCAC TCTGTCAGTT CAGCATCCAC 
CCTGTGGCGC AGGTCGCTCA CAATTACTGT GCCCACCTGA GAGAGCGGTG CCGACAGGTG 
GACACATATA GTCCCCAGGT GGGAACCCTC TTTCCTTGGG ACTCTGGCCC AGCCTTTGCT 
TTGCATCCTG AACCAGCCTT GCTTGGCTTG GGGAATCTGA CCAGAACTCC AGCAACCCTG 
GGGGCCAGGC CTGTAAGCAG GGCACTTGCA GTGACTCTGG CTCCTGCCAT GCCCACCAAG 
CCCCTGAGGA CTGTCCACCC AGATGTCTCT GAACACAGCT CTTCCCAGAC CCCACTGTCA 
CCTGCCAAAC AGTCGGCCAG GAAAACACCT TCCCCCTCCT CGTCAGCTTC CCTGGCTAAT 
TCCACCAGGG TTTATCGTCC AGCTGCTGCC CAGCCAAGAC AGATCACAAC TACTAGCCCC 
ACCAAACGTT CCCCCACCAA GCCTTCTGTT TCTCCCCTTT CAGTTACCCC CATGAAAAGT 
CCCCATGCAA CCCAGAAAAC AGGTGTCCCT TCATTCACAA AGCCTGTTCC ACCCACTCAG 
AAGCCAGCGC CCTTCACCTC CTACCTAGCA CCTTCCAAAG CCTCTAGTCC CACTGTGAGG 
CCTGTTCAGA AGACTTTCAT GACACCCCGA CCTCCAGTCC CCAGCCCCCA GCCCCTGCGC 
CCTACTACTG GCTTATCTAA GAAGTTCACT AACCCCACTG TTGCAAAATC TAAGTCCAAG 
ACGACCAGTT GGGCCAGCAA GCCGGTCTTG GCCCGCTCCA GTGTCCCTAA AACGCTTCAG 
CAAACTGTTT TGTCCCAGTC TCCTGTTTCC TATCTGGGCT CTCAGACACT GGCTCCAGCC 
CTCCCTCCTT TGGGTGTTGG AAATCCTAGA ACGATGCCTC CCACACGTGA CTCTGCTTTA 
ACTCCTGCTG GAAGCAAGAA ATTCACAGGA CGGGAAACCT CCAAGAAAAC CAGACAGAAG 
AGCAGCCCCC GGAAGCCCGA ACCTCTCAGC CCTGGGAAGT CAGCCAGGGA TGCCTCACCA 
AGAGACCTGA CAACCAAGCC TAGCCGGCCG TCCACCCCAG CCCTGGTCCT TGCCCCAGCT 
TACCTCCTGT CCTCCAGCCC CCAGCCCACC AGCAGCAGCT TCCCTTTCTT CCACCTGCTG 
GGCCCTACAC CTTTCCCGAT GCTGATGGGG CCTCCAGGCT CCAAGGGAGA CTGTGGCTTG 
CCGGGTCCCC CTGGGCTTCC AGGATTACCT GGATCTCCGG GCGCACGAGG TCCTCGGGGT 
CCTCCTGGGC CGTACGGGAA CCCAGGCCCA CCTGGCCCTC CAGGAGCCAA AGGACAGAAA 
GGGGACCCAG GACTCTCACC AGGACAGGCT CACGATGGAG CAAAGGGCAA CATGGGCTTG 
CCTGGGCTCT CCGGAAATCC AGGACCCTTG GGAAGGAAGG GACACAAGGG CCATCCTGGA 
GCAGCAGGGC ACCCTGGAGA ACAGGGGCAG CCAGGACCTG AGGGCAGTCC AGGGGCCAAA 
GGTTACCCTG GCAGGCAGGG GTTCCCTGGA CCTGTAGGAG ACCCTGGCCC CAAAGGCAGC 
AGGGGTTACA TTGGTCTTCC GGGGCTCTTT GGCCTGCCAG GGTCGGATGG AGAGCGGGGT 
CTCCCTGGCG TTCCTGGCAA GAGGGGCGAG ATGGGTAGGC CGGGCTTCCC TGGAGACTTT 
GGGGAAAGAG GCCCCCCTGG ACTTGATGGC AACCCTGGAG AAATTGGCCT GCCGGGGCCA 
CCAGGAGTGC TCGGGCTCAT TGGTGACACA GGAGCACTGG GCCCAGTTGG CTACCCAGGA 
CCGAAAGGCA TGAAGGGACT GATGGGCGGC GTGGGGGAGC CCGGACTGAA AGGTGATAAG 
GGTGAACAAG GGGTGCCAGG TGTGTCGGGA GATCCTGGCT TCCAAGGAGA CAAGGGGAGT 
CATGGCTTGC CAGGGCTCCC GGGTGGCCGG GGGAAGCCAG GGCCTCTGGG AAAAGCTGGA 
GACAAAGGAT CACTTGGGTT TCCTGGACCT CCTGGACCTG AGGGTTTCCC AGGAGACATT 
GGCCCTCCAG GGGACAATGG CCCTGAAGGC ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG 
CCTGGACCTC CTGGGCAGCT GGGGCCTGAG GGAGATGAAG GACCCATGGG GCCACCAGGA 
GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT 
GGCTCGAAGG GGGAACCAGG GGATCCTGGA CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT 
ATGGGATTCA TTGGTCTTGT CGGAGAGCCT GGAATTGTTG GGGAGAAGGG AGACCGTGGG 
GTGATGGGAC CCCCAGGTGC ACCTGGACCC AAGGGGTCGA TGGTAAGGAG GCCATCCTGG 
AACACCCGGT GGTATTGGGA ACCCTGGAGA GCCTGGACCC TGGGGCCCTC CAGGATCTCG 
AGGCCTGCCA GGCATGAGGG GAGCCAAGGG GCACCGGGGT CTCCAGCTGA CTTACACAGG 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006538102.1
CDS	509..3694
Translation	MGTGFARGARGTAASGPGGGFLFAWILVSFTCHLASTQGAPEDVDVLQRLGLSWTKAGGGRSPTPPGVIPFPSGFIFTQRAKLQAPTANVLPTTLGRELALVLSLCSHRVNHAFLFAIRSRKHKLQLGLQFLPGRTIIHLGPRQSVAFDLDVHDGRWHHLALELRGRTVTMVTACGQHRVPVPLPSRRDSMLDPQGSFLLGKVNPRAVQFEGALCQFSIHPVAQVAHNYCAHLRERCRQVDTYSPQVGTLFPWDSGPAFALHPEPALLGLGNLTRTPATLGARPVSRALAVTLAPAMPTKPLRTVHPDVSEHSSSQTPLSPAKQSARKTPSPSSSASLANSTRVYRPAAAQPRQITTTSPTKRSPTKPSVSPLSVTPMKSPHATQKTGVPSFTKPVPPTQKPAPFTSYLAPSKASSPTVRPVQKTFMTPRPPVPSPQPLRPTTGLSKKFTNPTVAKSKSKTTSWASKPVLARSSVPKTLQQTVLSQSPVSYLGSQTLAPALPPLGVGNPRTMPPTRDSALTPAGSKKFTGRETSKKTRQKSSPRKPEPLSPGKSARDASPRDLTTKPSRPSTPALVLAPAYLLSSSPQPTSSSFPFFHLLGPTPFPMLMGPPGSKGDCGLPGPPGLPGLPGSPGARGPRGPPGPYGNPGPPGPPGAKGQKGDPGLSPGQAHDGAKGNMGLPGLSGNPGPLGRKGHKGHPGAAGHPGEQGQPGPEGSPGAKGYPGRQGFPGPVGDPGPKGSRGYIGLPGLFGLPGSDGERGLPGVPGKRGEMGRPGFPGDFGERGPPGLDGNPGEIGLPGPPGVLGLIGDTGALGPVGYPGPKGMKGLMGGVGEPGLKGDKGEQGVPGVSGDPGFQGDKGSHGLPGLPGGRGKPGPLGKAGDKGSLGFPGPPGPEGFPGDIGPPGDNGPEGMKGKPGARGLPGPPGQLGPEGDEGPMGPPGVPGLEGQPGRKGFPGRPGLDGSKGEPGDPGRPGPVGEQGLMGFIGLVGEPGIVGEKGDRGVMGPPGAPGPKGSMVRRPSWNTRWYWEPWRAWTLGPSRISRPARHEGSQGAPGSPADLHRL

Target ORF information:

RefSeq Version	XM_006538039.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006538039.3

ORF Insert Sequence:

ATGGGCACGG GATTCGCGCG GGGGGCCCGA GGCACAGCGG CGTCAGGACC CGGGGGGGGG 
TTTCTCTTCG CCTGGATCTT GGTCTCATTT ACCTGTCACC TGGCCTCCAC CCAAGGAGCT 
CCTGAAGATG TGGATGTCCT CCAGCGGCTG GGCCTCAGCT GGACGAAGGC CGGGGGTGGC 
CGGAGTCCTA CACCCCCTGG TGTCATTCCT TTCCCATCTG GCTTCATCTT CACACAGCGG 
GCCAAGCTGC AGGCCCCCAC AGCCAATGTT CTTCCCACCA CCCTGGGGCG GGAGCTGGCA 
TTGGTGTTGA GCCTCTGTTC ACATCGTGTG AACCATGCCT TCCTCTTTGC CATCCGCAGC 
AGGAAACACA AGCTGCAACT GGGTCTGCAG TTCCTCCCCG GCAGGACGAT CATCCACCTG 
GGGCCTCGGC AATCAGTGGC CTTCGACTTG GATGTGCATG ATGGGCGCTG GCACCACCTG 
GCCCTAGAGT TGCGTGGCCG CACAGTCACA ATGGTGACAG CCTGTGGGCA GCACAGGGTA 
CCTGTCCCAC TGCCTTCCCG TAGGGACTCC ATGCTTGACC CCCAGGGCTC CTTTCTCTTG 
GGGAAGGTGA ACCCCCGTGC TGTCCAGTTT GAAGGTGCAC TCTGTCAGTT CAGCATCCAC 
CCTGTGGCGC AGGTCGCTCA CAATTACTGT GCCCACCTGA GAGAGCGGTG CCGACAGGTG 
GACACATATA GTCCCCAGGT GGGAACCCTC TTTCCTTGGG ACTCTGGCCC AGCCTTTGCT 
TTGCATCCTG AACCAGCCTT GCTTGGCTTG GGGAATCTGA CCAGAACTCC AGCAACCCTG 
GGGGCCAGGC CTGTAAGCAG GGCACTTGCA GTGACTCTGG CTCCTGCCAT GCCCACCAAG 
CCCCTGAGGA CTGTCCACCC AGATGTCTCT GAACACAGCT CTTCCCAGAC CCCACTGTCA 
CCTGCCAAAC AGTCGGCCAG GAAAACACCT TCCCCCTCCT CGTCAGCTTC CCTGGCTAAT 
TCCACCAGGG TTTATCGTCC AGCTGCTGCC CAGCCAAGAC AGATCACAAC TACTAGCCCC 
ACCAAACGTT CCCCCACCAA GCCTTCTGTT TCTCCCCTTT CAGTTACCCC CATGAAAAGT 
CCCCATGCAA CCCAGAAAAC AGGTGTCCCT TCATTCACAA AGCCTGTTCC ACCCACTCAG 
AAGCCAGCGC CCTTCACCTC CTACCTAGCA CCTTCCAAAG CCTCTAGTCC CACTGTGAGG 
CCTGTTCAGA AGACTTTCAT GACACCCCGA CCTCCAGTCC CCAGCCCCCA GCCCCTGCGC 
CCTACTACTG GCTTATCTAA GAAGTTCACT AACCCCACTG TTGCAAAATC TAAGTCCAAG 
ACGACCAGTT GGGCCAGCAA GCCGGTCTTG GCCCGCTCCA GTGTCCCTAA AACGCTTCAG 
CAAACTGTTT TGTCCCAGTC TCCTGTTTCC TATCTGGGCT CTCAGACACT GGCTCCAGCC 
CTCCCTCCTT TGGGTGTTGG AAATCCTAGA ACGATGCCTC CCACACGTGA CTCTGCTTTA 
ACTCCTGCTG GAAGCAAGAA ATTCACAGGA CGGGAAACCT CCAAGAAAAC CAGACAGAAG 
AGCAGCCCCC GGAAGCCCGA ACCTCTCAGC CCTGGGAAGT CAGCCAGGGA TGCCTCACCA 
AGAGACCTGA CAACCAAGCC TAGCCGGCCG TCCACCCCAG CCCTGGTCCT TGCCCCAGCT 
TACCTCCTGT CCTCCAGCCC CCAGCCCACC AGCAGCAGCT TCCCTTTCTT CCACCTGCTG 
GGCCCTACAC CTTTCCCGAT GCTGATGGGG CCTCCAGGCT CCAAGGGAGA CTGTGGCTTG 
CCGGGTCCCC CTGGGCTTCC AGGATTACCT GGATCTCCGG GCGCACGAGG TCCTCGGGGT 
CCTCCTGGGC CGTACGGGAA CCCAGGCCCA CCTGGCCCTC CAGGAGCCAA AGGACAGAAA 
GGGGACCCAG GACTCTCACC AGGACAGGCT CACGATGGAG CAAAGGGCAA CATGGGCTTG 
CCTGGGCTCT CCGGAAATCC AGGACCCTTG GGAAGGAAGG GACACAAGGG CCATCCTGGA 
GCAGCAGGGC ACCCTGGAGA ACAGGGGCAG CCAGGACCTG AGGGCAGTCC AGGGGCCAAA 
GGTTACCCTG GCAGGCAGGG GTTCCCTGGA CCTGTAGGAG ACCCTGGCCC CAAAGGCAGC 
AGGGGTTACA TTGGTCTTCC GGGGCTCTTT GGCCTGCCAG GGTCGGATGG AGAGCGGGGT 
CTCCCTGGCG TTCCTGGCAA GAGGGGCGAG ATGGGTAGGC CGGGCTTCCC TGGAGACTTT 
GGGGAAAGAG GCCCCCCTGG ACTTGATGGC AACCCTGGAG AAATTGGCCT GCCGGGGCCA 
CCAGGAGTGC TCGGGCTCAT TGGTGACACA GGAGCACTGG GCCCAGTTGG CTACCCAGGA 
CCGAAAGGCA TGAAGGGACT GATGGGCGGC GTGGGGGAGC CCGGACTGAA AGGTGATAAG 
GGTGAACAAG GGGTGCCAGG TGTGTCGGGA GATCCTGGCT TCCAAGGAGA CAAGGGGAGT 
CATGGCTTGC CAGGGCTCCC GGGTGGCCGG GGGAAGCCAG GGCCTCTGGG AAAAGCTGGA 
GACAAAGGAT CACTTGGGTT TCCTGGACCT CCTGGACCTG AGGGTTTCCC AGGAGACATT 
GGCCCTCCAG GGGACAATGG CCCTGAAGGC ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG 
CCTGGACCTC CTGGGCAGCT GGGGCCTGAG GGAGATGAAG GACCCATGGG GCCACCAGGA 
GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT 
GGCTCGAAGG GGGAACCAGG GGATCCTGGA CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT 
ATGGGATTCA TTGGTCTTGT CGGAGAGCCT GGAATTGTTG GGGAGAAGGG AGACCGTGGG 
GTGATGGGAC CCCCAGGTGC ACCTGGACCC AAGGGGTCGA TGGTAAGGAG GCCATCCTGG 
AACACCCGGT GGTATTGGGA ACCCTGGAGA GCCTGGACCC TGGGGCCCTC CAGGATCTCG 
AGGCCTGCCA GGCATGAGGG GAGCCAAGGG GCACCGGGGT CTCCAGCTGA CTTACACAGG 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col27a1 ( XM_006538040.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006538103.1 Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X11, mRNA.

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CloneID	OMu33010
Clone ID Related Accession (Same CDS sequence)	XM_006538040.3
Accession Version	XM_006538040.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3174bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	collagen alpha-1(XXVII) chain isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006538040.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCACGG GATTCGCGCG GGGGGCCCGA GGCACAGCGG CGTCAGGACC CGGGGGGGGG 
TTTCTCTTCG CCTGGATCTT GGTCTCATTT ACCTGTCACC TGGCCTCCAC CCAAGGAGCT 
CCTGAAGATG TGGATGTCCT CCAGCGGCTG GGCCTCAGCT GGACGAAGGC CGGGGGTGGC 
CGGAGTCCTA CACCCCCTGG TGTCATTCCT TTCCCATCTG GCTTCATCTT CACACAGCGG 
GCCAAGCTGC AGGCCCCCAC AGCCAATGTT CTTCCCACCA CCCTGGGGCG GGAGCTGGCA 
TTGGTGTTGA GCCTCTGTTC ACATCGTGTG AACCATGCCT TCCTCTTTGC CATCCGCAGC 
AGGAAACACA AGCTGCAACT GGGTCTGCAG TTCCTCCCCG GCAGGACGAT CATCCACCTG 
GGGCCTCGGC AATCAGTGGC CTTCGACTTG GATGTGCATG ATGGGCGCTG GCACCACCTG 
GCCCTAGAGT TGCGTGGCCG CACAGTCACA ATGGTGACAG CCTGTGGGCA GCACAGGGTA 
CCTGTCCCAC TGCCTTCCCG TAGGGACTCC ATGCTTGACC CCCAGGGCTC CTTTCTCTTG 
GGGAAGGTGA ACCCCCGTGC TGTCCAGTTT GAAGGTGCAC TCTGTCAGTT CAGCATCCAC 
CCTGTGGCGC AGGTCGCTCA CAATTACTGT GCCCACCTGA GAGAGCGGTG CCGACAGGTG 
GACACATATA GTCCCCAGGT GGGAACCCTC TTTCCTTGGG ACTCTGGCCC AGCCTTTGCT 
TTGCATCCTG AACCAGCCTT GCTTGGCTTG GGGAATCTGA CCAGAACTCC AGCAACCCTG 
GGGGCCAGGC CTGTAAGCAG GGCACTTGCA GTGACTCTGG CTCCTGCCAT GCCCACCAAG 
CCCCTGAGGA CTGTCCACCC AGATGTCTCT GAACACAGCT CTTCCCAGAC CCCACTGTCA 
CCTGCCAAAC AGTCGGCCAG GAAAACACCT TCCCCCTCCT CGTCAGCTTC CCTGGCTAAT 
TCCACCAGGG TTTATCGTCC AGCTGCTGCC CAGCCAAGAC AGATCACAAC TACTAGCCCC 
ACCAAACGTT CCCCCACCAA GCCTTCTGTT TCTCCCCTTT CAGTTACCCC CATGAAAAGT 
CCCCATGCAA CCCAGAAAAC AGGTGTCCCT TCATTCACAA AGCCTGTTCC ACCCACTCAG 
AAGCCAGCGC CCTTCACCTC CTACCTAGCA CCTTCCAAAG CCTCTAGTCC CACTGTGAGG 
CCTGTTCAGA AGACTTTCAT GACACCCCGA CCTCCAGTCC CCAGCCCCCA GCCCCTGCGC 
CCTACTACTG GCTTATCTAA GAAGTTCACT AACCCCACTG TTGCAAAATC TAAGTCCAAG 
ACGACCAGTT GGGCCAGCAA GCCGGTCTTG GCCCGCTCCA GTGTCCCTAA AACGCTTCAG 
CAAACTGTTT TGTCCCAGTC TCCTGTTTCC TATCTGGGCT CTCAGACACT GGCTCCAGCC 
CTCCCTCCTT TGGGTGTTGG AAATCCTAGA ACGATGCCTC CCACACGTGA CTCTGCTTTA 
ACTCCTGCTG GAAGCAAGAA ATTCACAGGA CGGGAAACCT CCAAGAAAAC CAGACAGAAG 
AGCAGCCCCC GGAAGCCCGA ACCTCTCAGC CCTGGGAAGT CAGCCAGGGA TGCCTCACCA 
AGAGACCTGA CAACCAAGCC TAGCCGGCCG TCCACCCCAG CCCTGGTCCT TGCCCCAGCT 
TACCTCCTGT CCTCCAGCCC CCAGCCCACC AGCAGCAGCT TCCCTTTCTT CCACCTGCTG 
GGCCCTACAC CTTTCCCGAT GCTGATGGGG CCTCCAGGCT CCAAGGGAGA CTGTGGCTTG 
CCGGGTCCCC CTGGGCTTCC AGGATTACCT GGATCTCCGG GCGCACGAGG TCCTCGGGGT 
CCTCCTGGGC CGTACGGGAA CCCAGGCCCA CCTGGCCCTC CAGGAGCCAA AGGACAGAAA 
GGGGACCCAG GACTCTCACC AGGACAGGCT CACGATGGAG CAAAGGGCAA CATGGGCTTG 
CCTGGGCTCT CCGGAAATCC AGGACCCTTG GGAAGGAAGG GACACAAGGG CCATCCTGGA 
GCAGCAGGGC ACCCTGGAGA ACAGGGGCAG CCAGGACCTG AGGGCAGTCC AGGGGCCAAA 
GGTTACCCTG GCAGGCAGGG GTTCCCTGGA CCTGTAGGAG ACCCTGGCCC CAAAGGCAGC 
AGGGGTTACA TTGGTCTTCC GGGGCTCTTT GGCCTGCCAG GGTCGGATGG AGAGCGGGGT 
CTCCCTGGCG TTCCTGGCAA GAGGGGCGAG ATGGGTAGGC CGGGCTTCCC TGGAGACTTT 
GGGGAAAGAG GCCCCCCTGG ACTTGATGGC AACCCTGGAG AAATTGGCCT GCCGGGGCCA 
CCAGGAGTGC TCGGGCTCAT TGGTGACACA GGAGCACTGG GCCCAGTTGG CTACCCAGGA 
CCGAAAGGCA TGAAGGGACT GATGGGCGGC GTGGGGGAGC CCGGACTGAA AGGTGATAAG 
GGTGAACAAG GGGTGCCAGG TGTGTCGGGA GATCCTGGCT TCCAAGGAGA CAAGGGGAGT 
CATGGCTTGC CAGGGCTCCC GGGTGGCCGG GGGAAGCCAG GGCCTCTGGG AAAAGCTGGA 
GACAAAGGAT CACTTGGGTT TCCTGGACCT CCTGGACCTG AGGGTTTCCC AGGAGACATT 
GGCCCTCCAG GGGACAATGG CCCTGAAGGC ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG 
CCTGGACCTC CTGGGCAGCT GGGGCCTGAG GGAGATGAAG GACCCATGGG GCCACCAGGA 
GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT 
GGCTCGAAGG GGGAACCAGG GGATCCTGGA CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT 
ATGGGATTCA TTGGTCTTGT CGGAGAGCCT GGAATTGTTG GGGAGAAGGG AGACCGTGGG 
GTGATGGGAC CCCCAGGTGC ACCTGGACCC AAGGGGTCGA TGGTAAGGAG GCCATCCTGG 
AACACCCGGT GGTATTGGGA ACCCTGGAGA GCCTGGACCC TGGGGCCCTC CAGGATCTCG 
AGGCCTGCCA GGCATGAGGG GAGCCAAGGG GCACCGGGGC CCTCGAGGAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006538103.1
CDS	509..3682
Translation	MGTGFARGARGTAASGPGGGFLFAWILVSFTCHLASTQGAPEDVDVLQRLGLSWTKAGGGRSPTPPGVIPFPSGFIFTQRAKLQAPTANVLPTTLGRELALVLSLCSHRVNHAFLFAIRSRKHKLQLGLQFLPGRTIIHLGPRQSVAFDLDVHDGRWHHLALELRGRTVTMVTACGQHRVPVPLPSRRDSMLDPQGSFLLGKVNPRAVQFEGALCQFSIHPVAQVAHNYCAHLRERCRQVDTYSPQVGTLFPWDSGPAFALHPEPALLGLGNLTRTPATLGARPVSRALAVTLAPAMPTKPLRTVHPDVSEHSSSQTPLSPAKQSARKTPSPSSSASLANSTRVYRPAAAQPRQITTTSPTKRSPTKPSVSPLSVTPMKSPHATQKTGVPSFTKPVPPTQKPAPFTSYLAPSKASSPTVRPVQKTFMTPRPPVPSPQPLRPTTGLSKKFTNPTVAKSKSKTTSWASKPVLARSSVPKTLQQTVLSQSPVSYLGSQTLAPALPPLGVGNPRTMPPTRDSALTPAGSKKFTGRETSKKTRQKSSPRKPEPLSPGKSARDASPRDLTTKPSRPSTPALVLAPAYLLSSSPQPTSSSFPFFHLLGPTPFPMLMGPPGSKGDCGLPGPPGLPGLPGSPGARGPRGPPGPYGNPGPPGPPGAKGQKGDPGLSPGQAHDGAKGNMGLPGLSGNPGPLGRKGHKGHPGAAGHPGEQGQPGPEGSPGAKGYPGRQGFPGPVGDPGPKGSRGYIGLPGLFGLPGSDGERGLPGVPGKRGEMGRPGFPGDFGERGPPGLDGNPGEIGLPGPPGVLGLIGDTGALGPVGYPGPKGMKGLMGGVGEPGLKGDKGEQGVPGVSGDPGFQGDKGSHGLPGLPGGRGKPGPLGKAGDKGSLGFPGPPGPEGFPGDIGPPGDNGPEGMKGKPGARGLPGPPGQLGPEGDEGPMGPPGVPGLEGQPGRKGFPGRPGLDGSKGEPGDPGRPGPVGEQGLMGFIGLVGEPGIVGEKGDRGVMGPPGAPGPKGSMVRRPSWNTRWYWEPWRAWTLGPSRISRPARHEGSQGAPGPSRT

Target ORF information:

RefSeq Version	XM_006538040.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006538040.3

ORF Insert Sequence:

ATGGGCACGG GATTCGCGCG GGGGGCCCGA GGCACAGCGG CGTCAGGACC CGGGGGGGGG 
TTTCTCTTCG CCTGGATCTT GGTCTCATTT ACCTGTCACC TGGCCTCCAC CCAAGGAGCT 
CCTGAAGATG TGGATGTCCT CCAGCGGCTG GGCCTCAGCT GGACGAAGGC CGGGGGTGGC 
CGGAGTCCTA CACCCCCTGG TGTCATTCCT TTCCCATCTG GCTTCATCTT CACACAGCGG 
GCCAAGCTGC AGGCCCCCAC AGCCAATGTT CTTCCCACCA CCCTGGGGCG GGAGCTGGCA 
TTGGTGTTGA GCCTCTGTTC ACATCGTGTG AACCATGCCT TCCTCTTTGC CATCCGCAGC 
AGGAAACACA AGCTGCAACT GGGTCTGCAG TTCCTCCCCG GCAGGACGAT CATCCACCTG 
GGGCCTCGGC AATCAGTGGC CTTCGACTTG GATGTGCATG ATGGGCGCTG GCACCACCTG 
GCCCTAGAGT TGCGTGGCCG CACAGTCACA ATGGTGACAG CCTGTGGGCA GCACAGGGTA 
CCTGTCCCAC TGCCTTCCCG TAGGGACTCC ATGCTTGACC CCCAGGGCTC CTTTCTCTTG 
GGGAAGGTGA ACCCCCGTGC TGTCCAGTTT GAAGGTGCAC TCTGTCAGTT CAGCATCCAC 
CCTGTGGCGC AGGTCGCTCA CAATTACTGT GCCCACCTGA GAGAGCGGTG CCGACAGGTG 
GACACATATA GTCCCCAGGT GGGAACCCTC TTTCCTTGGG ACTCTGGCCC AGCCTTTGCT 
TTGCATCCTG AACCAGCCTT GCTTGGCTTG GGGAATCTGA CCAGAACTCC AGCAACCCTG 
GGGGCCAGGC CTGTAAGCAG GGCACTTGCA GTGACTCTGG CTCCTGCCAT GCCCACCAAG 
CCCCTGAGGA CTGTCCACCC AGATGTCTCT GAACACAGCT CTTCCCAGAC CCCACTGTCA 
CCTGCCAAAC AGTCGGCCAG GAAAACACCT TCCCCCTCCT CGTCAGCTTC CCTGGCTAAT 
TCCACCAGGG TTTATCGTCC AGCTGCTGCC CAGCCAAGAC AGATCACAAC TACTAGCCCC 
ACCAAACGTT CCCCCACCAA GCCTTCTGTT TCTCCCCTTT CAGTTACCCC CATGAAAAGT 
CCCCATGCAA CCCAGAAAAC AGGTGTCCCT TCATTCACAA AGCCTGTTCC ACCCACTCAG 
AAGCCAGCGC CCTTCACCTC CTACCTAGCA CCTTCCAAAG CCTCTAGTCC CACTGTGAGG 
CCTGTTCAGA AGACTTTCAT GACACCCCGA CCTCCAGTCC CCAGCCCCCA GCCCCTGCGC 
CCTACTACTG GCTTATCTAA GAAGTTCACT AACCCCACTG TTGCAAAATC TAAGTCCAAG 
ACGACCAGTT GGGCCAGCAA GCCGGTCTTG GCCCGCTCCA GTGTCCCTAA AACGCTTCAG 
CAAACTGTTT TGTCCCAGTC TCCTGTTTCC TATCTGGGCT CTCAGACACT GGCTCCAGCC 
CTCCCTCCTT TGGGTGTTGG AAATCCTAGA ACGATGCCTC CCACACGTGA CTCTGCTTTA 
ACTCCTGCTG GAAGCAAGAA ATTCACAGGA CGGGAAACCT CCAAGAAAAC CAGACAGAAG 
AGCAGCCCCC GGAAGCCCGA ACCTCTCAGC CCTGGGAAGT CAGCCAGGGA TGCCTCACCA 
AGAGACCTGA CAACCAAGCC TAGCCGGCCG TCCACCCCAG CCCTGGTCCT TGCCCCAGCT 
TACCTCCTGT CCTCCAGCCC CCAGCCCACC AGCAGCAGCT TCCCTTTCTT CCACCTGCTG 
GGCCCTACAC CTTTCCCGAT GCTGATGGGG CCTCCAGGCT CCAAGGGAGA CTGTGGCTTG 
CCGGGTCCCC CTGGGCTTCC AGGATTACCT GGATCTCCGG GCGCACGAGG TCCTCGGGGT 
CCTCCTGGGC CGTACGGGAA CCCAGGCCCA CCTGGCCCTC CAGGAGCCAA AGGACAGAAA 
GGGGACCCAG GACTCTCACC AGGACAGGCT CACGATGGAG CAAAGGGCAA CATGGGCTTG 
CCTGGGCTCT CCGGAAATCC AGGACCCTTG GGAAGGAAGG GACACAAGGG CCATCCTGGA 
GCAGCAGGGC ACCCTGGAGA ACAGGGGCAG CCAGGACCTG AGGGCAGTCC AGGGGCCAAA 
GGTTACCCTG GCAGGCAGGG GTTCCCTGGA CCTGTAGGAG ACCCTGGCCC CAAAGGCAGC 
AGGGGTTACA TTGGTCTTCC GGGGCTCTTT GGCCTGCCAG GGTCGGATGG AGAGCGGGGT 
CTCCCTGGCG TTCCTGGCAA GAGGGGCGAG ATGGGTAGGC CGGGCTTCCC TGGAGACTTT 
GGGGAAAGAG GCCCCCCTGG ACTTGATGGC AACCCTGGAG AAATTGGCCT GCCGGGGCCA 
CCAGGAGTGC TCGGGCTCAT TGGTGACACA GGAGCACTGG GCCCAGTTGG CTACCCAGGA 
CCGAAAGGCA TGAAGGGACT GATGGGCGGC GTGGGGGAGC CCGGACTGAA AGGTGATAAG 
GGTGAACAAG GGGTGCCAGG TGTGTCGGGA GATCCTGGCT TCCAAGGAGA CAAGGGGAGT 
CATGGCTTGC CAGGGCTCCC GGGTGGCCGG GGGAAGCCAG GGCCTCTGGG AAAAGCTGGA 
GACAAAGGAT CACTTGGGTT TCCTGGACCT CCTGGACCTG AGGGTTTCCC AGGAGACATT 
GGCCCTCCAG GGGACAATGG CCCTGAAGGC ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG 
CCTGGACCTC CTGGGCAGCT GGGGCCTGAG GGAGATGAAG GACCCATGGG GCCACCAGGA 
GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT 
GGCTCGAAGG GGGAACCAGG GGATCCTGGA CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT 
ATGGGATTCA TTGGTCTTGT CGGAGAGCCT GGAATTGTTG GGGAGAAGGG AGACCGTGGG 
GTGATGGGAC CCCCAGGTGC ACCTGGACCC AAGGGGTCGA TGGTAAGGAG GCCATCCTGG 
AACACCCGGT GGTATTGGGA ACCCTGGAGA GCCTGGACCC TGGGGCCCTC CAGGATCTCG 
AGGCCTGCCA GGCATGAGGG GAGCCAAGGG GCACCGGGGC CCTCGAGGAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col27a1 ( XM_011250057.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011248359.1 Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X7, mRNA.

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CloneID	OMu74976
Clone ID Related Accession (Same CDS sequence)	XM_011250057.3 , XM_011250057.2
Accession Version	XM_011250057.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3708bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	collagen alpha-1(XXVII) chain isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011250057.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCACGG GATTCGCGCG GGGGGCCCGA GGCACAGCGG CGTCAGGACC CGGGGGGGGG 
TTTCTCTTCG CCTGGATCTT GGTCTCATTT ACCTGTCACC TGGCCTCCAC CCAAGGAGCT 
CCTGAAGATG TGGATGTCCT CCAGCGGCTG GGCCTCAGCT GGACGAAGGC CGGGGGTGGC 
CGGAGTCCTA CACCCCCTGG TGTCATTCCT TTCCCATCTG GCTTCATCTT CACACAGCGG 
GCCAAGCTGC AGGCCCCCAC AGCCAATGTT CTTCCCACCA CCCTGGGGCG GGAGCTGGCA 
TTGGTGTTGA GCCTCTGTTC ACATCGTGTG AACCATGCCT TCCTCTTTGC CATCCGCAGC 
AGGAAACACA AGCTGCAACT GGGTCTGCAG TTCCTCCCCG GCAGGACGAT CATCCACCTG 
GGGCCTCGGC AATCAGTGGC CTTCGACTTG GATGTGCATG ATGGGCGCTG GCACCACCTG 
GCCCTAGAGT TGCGTGGCCG CACAGTCACA ATGGTGACAG CCTGTGGGCA GCACAGGGTA 
CCTGTCCCAC TGCCTTCCCG TAGGGACTCC ATGCTTGACC CCCAGGGCTC CTTTCTCTTG 
GGGAAGGTGA ACCCCCGTGC TGTCCAGTTT GAAGGTGCAC TCTGTCAGTT CAGCATCCAC 
CCTGTGGCGC AGGTCGCTCA CAATTACTGT GCCCACCTGA GAGAGCGGTG CCGACAGGTG 
GACACATATA GTCCCCAGGT GGGAACCCTC TTTCCTTGGG ACTCTGGCCC AGCCTTTGCT 
TTGCATCCTG AACCAGCCTT GCTTGGCTTG GGGAATCTGA CCAGAACTCC AGCAACCCTG 
GGGGCCAGGC CTGTAAGCAG GGCACTTGCA GTGACTCTGG CTCCTGCCAT GCCCACCAAG 
CCCCTGAGGA CTGTCCACCC AGATGTCTCT GAACACAGCT CTTCCCAGAC CCCACTGTCA 
CCTGCCAAAC AGTCGGCCAG GAAAACACCT TCCCCCTCCT CGTCAGCTTC CCTGGCTAAT 
TCCACCAGGG TTTATCGTCC AGCTGCTGCC CAGCCAAGAC AGATCACAAC TACTAGCCCC 
ACCAAACGTT CCCCCACCAA GCCTTCTGTT TCTCCCCTTT CAGTTACCCC CATGAAAAGT 
CCCCATGCAA CCCAGAAAAC AGGTGTCCCT TCATTCACAA AGCCTGTTCC ACCCACTCAG 
AAGCCAGCGC CCTTCACCTC CTACCTAGCA CCTTCCAAAG CCTCTAGTCC CACTGTGAGG 
CCTGTTCAGA AGACTTTCAT GACACCCCGA CCTCCAGTCC CCAGCCCCCA GCCCCTGCGC 
CCTACTACTG GCTTATCTAA GAAGTTCACT AACCCCACTG TTGCAAAATC TAAGTCCAAG 
ACGACCAGTT GGGCCAGCAA GCCGGTCTTG GCCCGCTCCA GTGTCCCTAA AACGCTTCAG 
CAAACTGTTT TGTCCCAGTC TCCTGTTTCC TATCTGGGCT CTCAGACACT GGCTCCAGCC 
CTCCCTCCTT TGGGTGTTGG AAATCCTAGA ACGATGCCTC CCACACGTGA CTCTGCTTTA 
ACTCCTGCTG GAAGCAAGAA ATTCACAGGA CGGGAAACCT CCAAGAAAAC CAGACAGAAG 
AGCAGCCCCC GGAAGCCCGA ACCTCTCAGC CCTGGGAAGT CAGCCAGGGA TGCCTCACCA 
AGAGACCTGA CAACCAAGCC TAGCCGGCCG TCCACCCCAG CCCTGGTCCT TGCCCCAGCT 
TACCTCCTGT CCTCCAGCCC CCAGCCCACC AGCAGCAGCT TCCCTTTCTT CCACCTGCTG 
GGCCCTACAC CTTTCCCGAT GCTGATGGGG CCTCCAGGCT CCAAGGGAGA CTGTGGCTTG 
CCGGGTCCCC CTGGGCTTCC AGGATTACCT GGATCTCCGG GCGCACGAGG TCCTCGGGGT 
CCTCCTGGGC CGTACGGGAA CCCAGGCCCA CCTGGCCCTC CAGGAGCCAA AGGACAGAAA 
GGGGACCCAG GACTCTCACC AGGACAGGCT CACGATGGAG CAAAGGGCAA CATGGGCTTG 
CCTGGGCTCT CCGGAAATCC AGGACCCTTG GGAAGGAAGG GACACAAGGG CCATCCTGGA 
GCAGCAGGGC ACCCTGGAGA ACAGGGGCAG CCAGGACCTG AGGGCAGTCC AGGGGCCAAA 
GGTTACCCTG GCAGGCAGGG GTTCCCTGGA CCTGTAGGAG ACCCTGGCCC CAAAGGCAGC 
AGGGGTTACA TTGGTCTTCC GGGGCTCTTT GGCCTGCCAG GGTCGGATGG AGAGCGGGGT 
CTCCCTGGCG TTCCTGGCAA GAGGGGCGAG ATGGGTAGGC CGGGCTTCCC TGGAGACTTT 
GGGGAAAGAG GCCCCCCTGG ACTTGATGGC AACCCTGGAG AAATTGGCCT GCCGGGGCCA 
CCAGGAGTGC TCGGGCTCAT TGGTGACACA GGAGCACTGG GCCCAGTTGG CTACCCAGGA 
CCGAAAGGCA TGAAGGGACT GATGGGCGGC GTGGGGGAGC CCGGACTGAA AGGTGATAAG 
GGTGAACAAG GGGTGCCAGG TGTGTCGGGA GATCCTGGCT TCCAAGGAGA CAAGGGGAGT 
CATGGCTTGC CAGGGCTCCC GGGTGGCCGG GGGAAGCCAG GGCCTCTGGG AAAAGCTGGA 
GACAAAGGAT CACTTGGGTT TCCTGGACCT CCTGGACCTG AGGGTTTCCC AGGAGACATT 
GGCCCTCCAG GGGACAATGG CCCTGAAGGC ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG 
CCTGGACCTC CTGGGCAGCT GGGGCCTGAG GGAGATGAAG GACCCATGGG GCCACCAGGA 
GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT 
GGCTCGAAGG GGGAACCAGG GGATCCTGGA CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT 
ATGGGATTCA TTGGTCTTGT CGGAGAGCCT GGAATTGTTG GGGAGAAGGG AGACCGTGGG 
GTGATGGGAC CCCCAGGTGC ACCTGGACCC AAGGGGTCGA TGGGCCATCC TGGAACACCC 
GGTGGTATTG GGAACCCTGG AGAGCCTGGA CCCTGGGGCC CTCCAGGATC TCGAGGCCTG 
CCAGGCATGA GGGGAGCCAA GGGGCACCGG GGCCCTCGAG GACCTGATGG ACCAGCTGGG 
GAGCAGGGAT CCAAGGGCCT GAAGGGTCGT GTAGGACCTC GGGGCAGACC TGGCCAGCCA 
GGACAGCAGG GTGCAGCTGG TGAGCGAGGC CACTCAGGTG CAAAAGGCTT CCTTGGCATC 
CCTGGTCCCT CAGGCCCTCC AGGTGCCAAG GGCCTCCCAG GAGAACCGGG CTCTCAGGGA 
CCTCAGGGGC CAGTTGGTCC TCCAGGAGAA ATGGGGCCCA AGGGACCGCC GGGTGCAGTG 
GGAGAGCCTG GCCTTCCTGG GGACTCCGGA ATGAAGGGTG ACCTCGGACC TCTGGGCCCC 
CCTGGGGAGC AAGGTCTTAT TGGGCAGCGG GGAGAGCCAG GCCTTGAGGG AGACCATGGC 
CCTGTGGGGC CAGATGGGCT GAAGGGCGAC AGGGGCGACC CAGGGCCCGA TGGCGAGCAT 
GGTGAAAAGG GCCAGGAAGG GCTGAAAGGA GAGGATGGGT CCCCTGGGCC CCCGGGCATC 
ACTGGCGTCC CGATCCATCC ACAGACACTC CAGCTCAGAT GGTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011248359.1
CDS	510..4217
Translation	MGTGFARGARGTAASGPGGGFLFAWILVSFTCHLASTQGAPEDVDVLQRLGLSWTKAGGGRSPTPPGVIPFPSGFIFTQRAKLQAPTANVLPTTLGRELALVLSLCSHRVNHAFLFAIRSRKHKLQLGLQFLPGRTIIHLGPRQSVAFDLDVHDGRWHHLALELRGRTVTMVTACGQHRVPVPLPSRRDSMLDPQGSFLLGKVNPRAVQFEGALCQFSIHPVAQVAHNYCAHLRERCRQVDTYSPQVGTLFPWDSGPAFALHPEPALLGLGNLTRTPATLGARPVSRALAVTLAPAMPTKPLRTVHPDVSEHSSSQTPLSPAKQSARKTPSPSSSASLANSTRVYRPAAAQPRQITTTSPTKRSPTKPSVSPLSVTPMKSPHATQKTGVPSFTKPVPPTQKPAPFTSYLAPSKASSPTVRPVQKTFMTPRPPVPSPQPLRPTTGLSKKFTNPTVAKSKSKTTSWASKPVLARSSVPKTLQQTVLSQSPVSYLGSQTLAPALPPLGVGNPRTMPPTRDSALTPAGSKKFTGRETSKKTRQKSSPRKPEPLSPGKSARDASPRDLTTKPSRPSTPALVLAPAYLLSSSPQPTSSSFPFFHLLGPTPFPMLMGPPGSKGDCGLPGPPGLPGLPGSPGARGPRGPPGPYGNPGPPGPPGAKGQKGDPGLSPGQAHDGAKGNMGLPGLSGNPGPLGRKGHKGHPGAAGHPGEQGQPGPEGSPGAKGYPGRQGFPGPVGDPGPKGSRGYIGLPGLFGLPGSDGERGLPGVPGKRGEMGRPGFPGDFGERGPPGLDGNPGEIGLPGPPGVLGLIGDTGALGPVGYPGPKGMKGLMGGVGEPGLKGDKGEQGVPGVSGDPGFQGDKGSHGLPGLPGGRGKPGPLGKAGDKGSLGFPGPPGPEGFPGDIGPPGDNGPEGMKGKPGARGLPGPPGQLGPEGDEGPMGPPGVPGLEGQPGRKGFPGRPGLDGSKGEPGDPGRPGPVGEQGLMGFIGLVGEPGIVGEKGDRGVMGPPGAPGPKGSMGHPGTPGGIGNPGEPGPWGPPGSRGLPGMRGAKGHRGPRGPDGPAGEQGSKGLKGRVGPRGRPGQPGQQGAAGERGHSGAKGFLGIPGPSGPPGAKGLPGEPGSQGPQGPVGPPGEMGPKGPPGAVGEPGLPGDSGMKGDLGPLGPPGEQGLIGQRGEPGLEGDHGPVGPDGLKGDRGDPGPDGEHGEKGQEGLKGEDGSPGPPGITGVPIHPQTLQLRWS

Target ORF information:

RefSeq Version	XM_011250057.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011250057.2

ORF Insert Sequence:

ATGGGCACGG GATTCGCGCG GGGGGCCCGA GGCACAGCGG CGTCAGGACC CGGGGGGGGG 
TTTCTCTTCG CCTGGATCTT GGTCTCATTT ACCTGTCACC TGGCCTCCAC CCAAGGAGCT 
CCTGAAGATG TGGATGTCCT CCAGCGGCTG GGCCTCAGCT GGACGAAGGC CGGGGGTGGC 
CGGAGTCCTA CACCCCCTGG TGTCATTCCT TTCCCATCTG GCTTCATCTT CACACAGCGG 
GCCAAGCTGC AGGCCCCCAC AGCCAATGTT CTTCCCACCA CCCTGGGGCG GGAGCTGGCA 
TTGGTGTTGA GCCTCTGTTC ACATCGTGTG AACCATGCCT TCCTCTTTGC CATCCGCAGC 
AGGAAACACA AGCTGCAACT GGGTCTGCAG TTCCTCCCCG GCAGGACGAT CATCCACCTG 
GGGCCTCGGC AATCAGTGGC CTTCGACTTG GATGTGCATG ATGGGCGCTG GCACCACCTG 
GCCCTAGAGT TGCGTGGCCG CACAGTCACA ATGGTGACAG CCTGTGGGCA GCACAGGGTA 
CCTGTCCCAC TGCCTTCCCG TAGGGACTCC ATGCTTGACC CCCAGGGCTC CTTTCTCTTG 
GGGAAGGTGA ACCCCCGTGC TGTCCAGTTT GAAGGTGCAC TCTGTCAGTT CAGCATCCAC 
CCTGTGGCGC AGGTCGCTCA CAATTACTGT GCCCACCTGA GAGAGCGGTG CCGACAGGTG 
GACACATATA GTCCCCAGGT GGGAACCCTC TTTCCTTGGG ACTCTGGCCC AGCCTTTGCT 
TTGCATCCTG AACCAGCCTT GCTTGGCTTG GGGAATCTGA CCAGAACTCC AGCAACCCTG 
GGGGCCAGGC CTGTAAGCAG GGCACTTGCA GTGACTCTGG CTCCTGCCAT GCCCACCAAG 
CCCCTGAGGA CTGTCCACCC AGATGTCTCT GAACACAGCT CTTCCCAGAC CCCACTGTCA 
CCTGCCAAAC AGTCGGCCAG GAAAACACCT TCCCCCTCCT CGTCAGCTTC CCTGGCTAAT 
TCCACCAGGG TTTATCGTCC AGCTGCTGCC CAGCCAAGAC AGATCACAAC TACTAGCCCC 
ACCAAACGTT CCCCCACCAA GCCTTCTGTT TCTCCCCTTT CAGTTACCCC CATGAAAAGT 
CCCCATGCAA CCCAGAAAAC AGGTGTCCCT TCATTCACAA AGCCTGTTCC ACCCACTCAG 
AAGCCAGCGC CCTTCACCTC CTACCTAGCA CCTTCCAAAG CCTCTAGTCC CACTGTGAGG 
CCTGTTCAGA AGACTTTCAT GACACCCCGA CCTCCAGTCC CCAGCCCCCA GCCCCTGCGC 
CCTACTACTG GCTTATCTAA GAAGTTCACT AACCCCACTG TTGCAAAATC TAAGTCCAAG 
ACGACCAGTT GGGCCAGCAA GCCGGTCTTG GCCCGCTCCA GTGTCCCTAA AACGCTTCAG 
CAAACTGTTT TGTCCCAGTC TCCTGTTTCC TATCTGGGCT CTCAGACACT GGCTCCAGCC 
CTCCCTCCTT TGGGTGTTGG AAATCCTAGA ACGATGCCTC CCACACGTGA CTCTGCTTTA 
ACTCCTGCTG GAAGCAAGAA ATTCACAGGA CGGGAAACCT CCAAGAAAAC CAGACAGAAG 
AGCAGCCCCC GGAAGCCCGA ACCTCTCAGC CCTGGGAAGT CAGCCAGGGA TGCCTCACCA 
AGAGACCTGA CAACCAAGCC TAGCCGGCCG TCCACCCCAG CCCTGGTCCT TGCCCCAGCT 
TACCTCCTGT CCTCCAGCCC CCAGCCCACC AGCAGCAGCT TCCCTTTCTT CCACCTGCTG 
GGCCCTACAC CTTTCCCGAT GCTGATGGGG CCTCCAGGCT CCAAGGGAGA CTGTGGCTTG 
CCGGGTCCCC CTGGGCTTCC AGGATTACCT GGATCTCCGG GCGCACGAGG TCCTCGGGGT 
CCTCCTGGGC CGTACGGGAA CCCAGGCCCA CCTGGCCCTC CAGGAGCCAA AGGACAGAAA 
GGGGACCCAG GACTCTCACC AGGACAGGCT CACGATGGAG CAAAGGGCAA CATGGGCTTG 
CCTGGGCTCT CCGGAAATCC AGGACCCTTG GGAAGGAAGG GACACAAGGG CCATCCTGGA 
GCAGCAGGGC ACCCTGGAGA ACAGGGGCAG CCAGGACCTG AGGGCAGTCC AGGGGCCAAA 
GGTTACCCTG GCAGGCAGGG GTTCCCTGGA CCTGTAGGAG ACCCTGGCCC CAAAGGCAGC 
AGGGGTTACA TTGGTCTTCC GGGGCTCTTT GGCCTGCCAG GGTCGGATGG AGAGCGGGGT 
CTCCCTGGCG TTCCTGGCAA GAGGGGCGAG ATGGGTAGGC CGGGCTTCCC TGGAGACTTT 
GGGGAAAGAG GCCCCCCTGG ACTTGATGGC AACCCTGGAG AAATTGGCCT GCCGGGGCCA 
CCAGGAGTGC TCGGGCTCAT TGGTGACACA GGAGCACTGG GCCCAGTTGG CTACCCAGGA 
CCGAAAGGCA TGAAGGGACT GATGGGCGGC GTGGGGGAGC CCGGACTGAA AGGTGATAAG 
GGTGAACAAG GGGTGCCAGG TGTGTCGGGA GATCCTGGCT TCCAAGGAGA CAAGGGGAGT 
CATGGCTTGC CAGGGCTCCC GGGTGGCCGG GGGAAGCCAG GGCCTCTGGG AAAAGCTGGA 
GACAAAGGAT CACTTGGGTT TCCTGGACCT CCTGGACCTG AGGGTTTCCC AGGAGACATT 
GGCCCTCCAG GGGACAATGG CCCTGAAGGC ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG 
CCTGGACCTC CTGGGCAGCT GGGGCCTGAG GGAGATGAAG GACCCATGGG GCCACCAGGA 
GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT 
GGCTCGAAGG GGGAACCAGG GGATCCTGGA CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT 
ATGGGATTCA TTGGTCTTGT CGGAGAGCCT GGAATTGTTG GGGAGAAGGG AGACCGTGGG 
GTGATGGGAC CCCCAGGTGC ACCTGGACCC AAGGGGTCGA TGGGCCATCC TGGAACACCC 
GGTGGTATTG GGAACCCTGG AGAGCCTGGA CCCTGGGGCC CTCCAGGATC TCGAGGCCTG 
CCAGGCATGA GGGGAGCCAA GGGGCACCGG GGCCCTCGAG GACCTGATGG ACCAGCTGGG 
GAGCAGGGAT CCAAGGGCCT GAAGGGTCGT GTAGGACCTC GGGGCAGACC TGGCCAGCCA 
GGACAGCAGG GTGCAGCTGG TGAGCGAGGC CACTCAGGTG CAAAAGGCTT CCTTGGCATC 
CCTGGTCCCT CAGGCCCTCC AGGTGCCAAG GGCCTCCCAG GAGAACCGGG CTCTCAGGGA 
CCTCAGGGGC CAGTTGGTCC TCCAGGAGAA ATGGGGCCCA AGGGACCGCC GGGTGCAGTG 
GGAGAGCCTG GCCTTCCTGG GGACTCCGGA ATGAAGGGTG ACCTCGGACC TCTGGGCCCC 
CCTGGGGAGC AAGGTCTTAT TGGGCAGCGG GGAGAGCCAG GCCTTGAGGG AGACCATGGC 
CCTGTGGGGC CAGATGGGCT GAAGGGCGAC AGGGGCGACC CAGGGCCCGA TGGCGAGCAT 
GGTGAAAAGG GCCAGGAAGG GCTGAAAGGA GAGGATGGGT CCCCTGGGCC CCCGGGCATC 
ACTGGCGTCC CGATCCATCC ACAGACACTC CAGCTCAGAT GGTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col27a1 ( XM_011250058.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011248360.1 Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OMu74977
Clone ID Related Accession (Same CDS sequence)	XM_011250058.3 , XM_011250058.2
Accession Version	XM_011250058.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3186bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	collagen alpha-1(XXVII) chain isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011250058.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCACGG GATTCGCGCG GGGGGCCCGA GGCACAGCGG CGTCAGGACC CGGGGGGGGG 
TTTCTCTTCG CCTGGATCTT GGTCTCATTT ACCTGTCACC TGGCCTCCAC CCAAGGAGCT 
CCTGAAGATG TGGATGTCCT CCAGCGGCTG GGCCTCAGCT GGACGAAGGC CGGGGGTGGC 
CGGAGTCCTA CACCCCCTGG TGTCATTCCT TTCCCATCTG GCTTCATCTT CACACAGCGG 
GCCAAGCTGC AGGCCCCCAC AGCCAATGTT CTTCCCACCA CCCTGGGGCG GGAGCTGGCA 
TTGGTGTTGA GCCTCTGTTC ACATCGTGTG AACCATGCCT TCCTCTTTGC CATCCGCAGC 
AGGAAACACA AGCTGCAACT GGGTCTGCAG TTCCTCCCCG GCAGGACGAT CATCCACCTG 
GGGCCTCGGC AATCAGTGGC CTTCGACTTG GATGTGCATG ATGGGCGCTG GCACCACCTG 
GCCCTAGAGT TGCGTGGCCG CACAGTCACA ATGGTGACAG CCTGTGGGCA GCACAGGGTA 
CCTGTCCCAC TGCCTTCCCG TAGGGACTCC ATGCTTGACC CCCAGGGCTC CTTTCTCTTG 
GGGAAGGTGA ACCCCCGTGC TGTCCAGTTT GAAGGTGCAC TCTGTCAGTT CAGCATCCAC 
CCTGTGGCGC AGGTCGCTCA CAATTACTGT GCCCACCTGA GAGAGCGGTG CCGACAGGTG 
GACACATATA GTCCCCAGGT GGGAACCCTC TTTCCTTGGG ACTCTGGCCC AGCCTTTGCT 
TTGCATCCTG AACCAGCCTT GCTTGGCTTG GGGAATCTGA CCAGAACTCC AGCAACCCTG 
GGGGCCAGGC CTGTAAGCAG GGCACTTGCA GTGACTCTGG CTCCTGCCAT GCCCACCAAG 
CCCCTGAGGA CTGTCCACCC AGATGTCTCT GAACACAGCT CTTCCCAGAC CCCACTGTCA 
CCTGCCAAAC AGTCGGCCAG GAAAACACCT TCCCCCTCCT CGTCAGCTTC CCTGGCTAAT 
TCCACCAGGG TTTATCGTCC AGCTGCTGCC CAGCCAAGAC AGATCACAAC TACTAGCCCC 
ACCAAACGTT CCCCCACCAA GCCTTCTGTT TCTCCCCTTT CAGTTACCCC CATGAAAAGT 
CCCCATGCAA CCCAGAAAAC AGGTGTCCCT TCATTCACAA AGCCTGTTCC ACCCACTCAG 
AAGCCAGCGC CCTTCACCTC CTACCTAGCA CCTTCCAAAG CCTCTAGTCC CACTGTGAGG 
CCTGTTCAGA AGACTTTCAT GACACCCCGA CCTCCAGTCC CCAGCCCCCA GCCCCTGCGC 
CCTACTACTG GCTTATCTAA GAAGTTCACT AACCCCACTG TTGCAAAATC TAAGTCCAAG 
ACGACCAGTT GGGCCAGCAA GCCGGTCTTG GCCCGCTCCA GTGTCCCTAA AACGCTTCAG 
CAAACTGTTT TGTCCCAGTC TCCTGTTTCC TATCTGGGCT CTCAGACACT GGCTCCAGCC 
CTCCCTCCTT TGGGTGTTGG AAATCCTAGA ACGATGCCTC CCACACGTGA CTCTGCTTTA 
ACTCCTGCTG GAAGCAAGAA ATTCACAGGA CGGGAAACCT CCAAGAAAAC CAGACAGAAG 
AGCAGCCCCC GGAAGCCCGA ACCTCTCAGC CCTGGGAAGT CAGCCAGGGA TGCCTCACCA 
AGAGACCTGA CAACCAAGCC TAGCCGGCCG TCCACCCCAG CCCTGGTCCT TGCCCCAGCT 
TACCTCCTGT CCTCCAGCCC CCAGCCCACC AGCAGCAGCT TCCCTTTCTT CCACCTGCTG 
GGCCCTACAC CTTTCCCGAT GCTGATGGGG CCTCCAGGCT CCAAGGGAGA CTGTGGCTTG 
CCGGGTCCCC CTGGGCTTCC AGGATTACCT GGATCTCCGG GCGCACGAGG TCCTCGGGGT 
CCTCCTGGGC CGTACGGGAA CCCAGGCCCA CCTGGCCCTC CAGGAGCCAA AGGACAGAAA 
GGGGACCCAG GACTCTCACC AGGACAGGCT CACGATGGAG CAAAGGGCAA CATGGGCTTG 
CCTGGGCTCT CCGGAAATCC AGGACCCTTG GGAAGGAAGG GACACAAGGG CCATCCTGGA 
GCAGCAGGGC ACCCTGGAGA ACAGGGGCAG CCAGGACCTG AGGGCAGTCC AGGGGCCAAA 
GGTTACCCTG GCAGGCAGGG GTTCCCTGGA CCTGTAGGAG ACCCTGGCCC CAAAGGCAGC 
AGGGGTTACA TTGGTCTTCC GGGGCTCTTT GGCCTGCCAG GGTCGGATGG AGAGCGGGGT 
CTCCCTGGCG TTCCTGGCAA GAGGGGCGAG ATGGGTAGGC CGGGCTTCCC TGGAGACTTT 
GGGGAAAGAG GCCCCCCTGG ACTTGATGGC AACCCTGGAG AAATTGGCCT GCCGGGGCCA 
CCAGGAGTGC TCGGGCTCAT TGGTGACACA GGAGCACTGG GCCCAGTTGG CTACCCAGGA 
CCGAAAGGCA TGAAGGGACT GATGGGCGGC GTGGGGGAGC CCGGACTGAA AGGTGATAAG 
GGTGAACAAG GGGTGCCAGG TGTGTCGGGA GATCCTGGCT TCCAAGGAGA CAAGGGGAGT 
CATGGCTTGC CAGGGCTCCC GGGTGGCCGG GGGAAGCCAG GGCCTCTGGG AAAAGCTGGA 
GACAAAGGAT CACTTGGGTT TCCTGGACCT CCTGGACCTG AGGGTTTCCC AGGAGACATT 
GGCCCTCCAG GGGACAATGG CCCTGAAGGC ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG 
CCTGGACCTC CTGGGCAGCT GGGGCCTGAG GGAGATGAAG GACCCATGGG GCCACCAGGA 
GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT 
GGCTCGAAGG GGGAACCAGG GGATCCTGGA CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT 
ATGGGATTCA TTGGTCTTGT CGGAGAGCCT GGAATTGTTG GGGAGAAGGG AGACCGTGGG 
GTGATGGGAC CCCCAGGTGC ACCTGGACCC AAGGGGTCGA TGGGCCATCC TGGAACACCC 
GGTGGTATTG GGAACCCTGG AGAGCCTGGA CCCTGGGGCC CTCCAGGATC TCGAGGCCTG 
CCAGGCATGA GGGGAGCCAA GGGGCACCGG GGTCTCCAGC TGACTTACAC AGGCTGTGAC 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011248360.1
CDS	509..3694
Translation	MGTGFARGARGTAASGPGGGFLFAWILVSFTCHLASTQGAPEDVDVLQRLGLSWTKAGGGRSPTPPGVIPFPSGFIFTQRAKLQAPTANVLPTTLGRELALVLSLCSHRVNHAFLFAIRSRKHKLQLGLQFLPGRTIIHLGPRQSVAFDLDVHDGRWHHLALELRGRTVTMVTACGQHRVPVPLPSRRDSMLDPQGSFLLGKVNPRAVQFEGALCQFSIHPVAQVAHNYCAHLRERCRQVDTYSPQVGTLFPWDSGPAFALHPEPALLGLGNLTRTPATLGARPVSRALAVTLAPAMPTKPLRTVHPDVSEHSSSQTPLSPAKQSARKTPSPSSSASLANSTRVYRPAAAQPRQITTTSPTKRSPTKPSVSPLSVTPMKSPHATQKTGVPSFTKPVPPTQKPAPFTSYLAPSKASSPTVRPVQKTFMTPRPPVPSPQPLRPTTGLSKKFTNPTVAKSKSKTTSWASKPVLARSSVPKTLQQTVLSQSPVSYLGSQTLAPALPPLGVGNPRTMPPTRDSALTPAGSKKFTGRETSKKTRQKSSPRKPEPLSPGKSARDASPRDLTTKPSRPSTPALVLAPAYLLSSSPQPTSSSFPFFHLLGPTPFPMLMGPPGSKGDCGLPGPPGLPGLPGSPGARGPRGPPGPYGNPGPPGPPGAKGQKGDPGLSPGQAHDGAKGNMGLPGLSGNPGPLGRKGHKGHPGAAGHPGEQGQPGPEGSPGAKGYPGRQGFPGPVGDPGPKGSRGYIGLPGLFGLPGSDGERGLPGVPGKRGEMGRPGFPGDFGERGPPGLDGNPGEIGLPGPPGVLGLIGDTGALGPVGYPGPKGMKGLMGGVGEPGLKGDKGEQGVPGVSGDPGFQGDKGSHGLPGLPGGRGKPGPLGKAGDKGSLGFPGPPGPEGFPGDIGPPGDNGPEGMKGKPGARGLPGPPGQLGPEGDEGPMGPPGVPGLEGQPGRKGFPGRPGLDGSKGEPGDPGRPGPVGEQGLMGFIGLVGEPGIVGEKGDRGVMGPPGAPGPKGSMGHPGTPGGIGNPGEPGPWGPPGSRGLPGMRGAKGHRGLQLTYTGCDL

Target ORF information:

RefSeq Version	XM_011250058.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011250058.2

ORF Insert Sequence:

ATGGGCACGG GATTCGCGCG GGGGGCCCGA GGCACAGCGG CGTCAGGACC CGGGGGGGGG 
TTTCTCTTCG CCTGGATCTT GGTCTCATTT ACCTGTCACC TGGCCTCCAC CCAAGGAGCT 
CCTGAAGATG TGGATGTCCT CCAGCGGCTG GGCCTCAGCT GGACGAAGGC CGGGGGTGGC 
CGGAGTCCTA CACCCCCTGG TGTCATTCCT TTCCCATCTG GCTTCATCTT CACACAGCGG 
GCCAAGCTGC AGGCCCCCAC AGCCAATGTT CTTCCCACCA CCCTGGGGCG GGAGCTGGCA 
TTGGTGTTGA GCCTCTGTTC ACATCGTGTG AACCATGCCT TCCTCTTTGC CATCCGCAGC 
AGGAAACACA AGCTGCAACT GGGTCTGCAG TTCCTCCCCG GCAGGACGAT CATCCACCTG 
GGGCCTCGGC AATCAGTGGC CTTCGACTTG GATGTGCATG ATGGGCGCTG GCACCACCTG 
GCCCTAGAGT TGCGTGGCCG CACAGTCACA ATGGTGACAG CCTGTGGGCA GCACAGGGTA 
CCTGTCCCAC TGCCTTCCCG TAGGGACTCC ATGCTTGACC CCCAGGGCTC CTTTCTCTTG 
GGGAAGGTGA ACCCCCGTGC TGTCCAGTTT GAAGGTGCAC TCTGTCAGTT CAGCATCCAC 
CCTGTGGCGC AGGTCGCTCA CAATTACTGT GCCCACCTGA GAGAGCGGTG CCGACAGGTG 
GACACATATA GTCCCCAGGT GGGAACCCTC TTTCCTTGGG ACTCTGGCCC AGCCTTTGCT 
TTGCATCCTG AACCAGCCTT GCTTGGCTTG GGGAATCTGA CCAGAACTCC AGCAACCCTG 
GGGGCCAGGC CTGTAAGCAG GGCACTTGCA GTGACTCTGG CTCCTGCCAT GCCCACCAAG 
CCCCTGAGGA CTGTCCACCC AGATGTCTCT GAACACAGCT CTTCCCAGAC CCCACTGTCA 
CCTGCCAAAC AGTCGGCCAG GAAAACACCT TCCCCCTCCT CGTCAGCTTC CCTGGCTAAT 
TCCACCAGGG TTTATCGTCC AGCTGCTGCC CAGCCAAGAC AGATCACAAC TACTAGCCCC 
ACCAAACGTT CCCCCACCAA GCCTTCTGTT TCTCCCCTTT CAGTTACCCC CATGAAAAGT 
CCCCATGCAA CCCAGAAAAC AGGTGTCCCT TCATTCACAA AGCCTGTTCC ACCCACTCAG 
AAGCCAGCGC CCTTCACCTC CTACCTAGCA CCTTCCAAAG CCTCTAGTCC CACTGTGAGG 
CCTGTTCAGA AGACTTTCAT GACACCCCGA CCTCCAGTCC CCAGCCCCCA GCCCCTGCGC 
CCTACTACTG GCTTATCTAA GAAGTTCACT AACCCCACTG TTGCAAAATC TAAGTCCAAG 
ACGACCAGTT GGGCCAGCAA GCCGGTCTTG GCCCGCTCCA GTGTCCCTAA AACGCTTCAG 
CAAACTGTTT TGTCCCAGTC TCCTGTTTCC TATCTGGGCT CTCAGACACT GGCTCCAGCC 
CTCCCTCCTT TGGGTGTTGG AAATCCTAGA ACGATGCCTC CCACACGTGA CTCTGCTTTA 
ACTCCTGCTG GAAGCAAGAA ATTCACAGGA CGGGAAACCT CCAAGAAAAC CAGACAGAAG 
AGCAGCCCCC GGAAGCCCGA ACCTCTCAGC CCTGGGAAGT CAGCCAGGGA TGCCTCACCA 
AGAGACCTGA CAACCAAGCC TAGCCGGCCG TCCACCCCAG CCCTGGTCCT TGCCCCAGCT 
TACCTCCTGT CCTCCAGCCC CCAGCCCACC AGCAGCAGCT TCCCTTTCTT CCACCTGCTG 
GGCCCTACAC CTTTCCCGAT GCTGATGGGG CCTCCAGGCT CCAAGGGAGA CTGTGGCTTG 
CCGGGTCCCC CTGGGCTTCC AGGATTACCT GGATCTCCGG GCGCACGAGG TCCTCGGGGT 
CCTCCTGGGC CGTACGGGAA CCCAGGCCCA CCTGGCCCTC CAGGAGCCAA AGGACAGAAA 
GGGGACCCAG GACTCTCACC AGGACAGGCT CACGATGGAG CAAAGGGCAA CATGGGCTTG 
CCTGGGCTCT CCGGAAATCC AGGACCCTTG GGAAGGAAGG GACACAAGGG CCATCCTGGA 
GCAGCAGGGC ACCCTGGAGA ACAGGGGCAG CCAGGACCTG AGGGCAGTCC AGGGGCCAAA 
GGTTACCCTG GCAGGCAGGG GTTCCCTGGA CCTGTAGGAG ACCCTGGCCC CAAAGGCAGC 
AGGGGTTACA TTGGTCTTCC GGGGCTCTTT GGCCTGCCAG GGTCGGATGG AGAGCGGGGT 
CTCCCTGGCG TTCCTGGCAA GAGGGGCGAG ATGGGTAGGC CGGGCTTCCC TGGAGACTTT 
GGGGAAAGAG GCCCCCCTGG ACTTGATGGC AACCCTGGAG AAATTGGCCT GCCGGGGCCA 
CCAGGAGTGC TCGGGCTCAT TGGTGACACA GGAGCACTGG GCCCAGTTGG CTACCCAGGA 
CCGAAAGGCA TGAAGGGACT GATGGGCGGC GTGGGGGAGC CCGGACTGAA AGGTGATAAG 
GGTGAACAAG GGGTGCCAGG TGTGTCGGGA GATCCTGGCT TCCAAGGAGA CAAGGGGAGT 
CATGGCTTGC CAGGGCTCCC GGGTGGCCGG GGGAAGCCAG GGCCTCTGGG AAAAGCTGGA 
GACAAAGGAT CACTTGGGTT TCCTGGACCT CCTGGACCTG AGGGTTTCCC AGGAGACATT 
GGCCCTCCAG GGGACAATGG CCCTGAAGGC ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG 
CCTGGACCTC CTGGGCAGCT GGGGCCTGAG GGAGATGAAG GACCCATGGG GCCACCAGGA 
GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT 
GGCTCGAAGG GGGAACCAGG GGATCCTGGA CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT 
ATGGGATTCA TTGGTCTTGT CGGAGAGCCT GGAATTGTTG GGGAGAAGGG AGACCGTGGG 
GTGATGGGAC CCCCAGGTGC ACCTGGACCC AAGGGGTCGA TGGGCCATCC TGGAACACCC 
GGTGGTATTG GGAACCCTGG AGAGCCTGGA CCCTGGGGCC CTCCAGGATC TCGAGGCCTG 
CCAGGCATGA GGGGAGCCAA GGGGCACCGG GGTCTCCAGC TGACTTACAC AGGCTGTGAC 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col27a1 ( XM_006538035.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006538098.1 Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X1, mRNA.

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CloneID	OMu33005
Clone ID Related Accession (Same CDS sequence)	XM_006538035.4 , XM_006538035.3
Accession Version	XM_006538035.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5541bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	collagen alpha-1(XXVII) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006538035.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCACGG GATTCGCGCG GGGGGCCCGA GGCACAGCGG CGTCAGGACC CGGGGGGGGG 
TTTCTCTTCG CCTGGATCTT GGTCTCATTT ACCTGTCACC TGGCCTCCAC CCAAGGAGCT 
CCTGAAGATG TGGATGTCCT CCAGCGGCTG GGCCTCAGCT GGACGAAGGC CGGGGGTGGC 
CGGAGTCCTA CACCCCCTGG TGTCATTCCT TTCCCATCTG GCTTCATCTT CACACAGCGG 
GCCAAGCTGC AGGCCCCCAC AGCCAATGTT CTTCCCACCA CCCTGGGGCG GGAGCTGGCA 
TTGGTGTTGA GCCTCTGTTC ACATCGTGTG AACCATGCCT TCCTCTTTGC CATCCGCAGC 
AGGAAACACA AGCTGCAACT GGGTCTGCAG TTCCTCCCCG GCAGGACGAT CATCCACCTG 
GGGCCTCGGC AATCAGTGGC CTTCGACTTG GATGTGCATG ATGGGCGCTG GCACCACCTG 
GCCCTAGAGT TGCGTGGCCG CACAGTCACA ATGGTGACAG CCTGTGGGCA GCACAGGGTA 
CCTGTCCCAC TGCCTTCCCG TAGGGACTCC ATGCTTGACC CCCAGGGCTC CTTTCTCTTG 
GGGAAGGTGA ACCCCCGTGC TGTCCAGTTT GAAGGTGCAC TCTGTCAGTT CAGCATCCAC 
CCTGTGGCGC AGGTCGCTCA CAATTACTGT GCCCACCTGA GAGAGCGGTG CCGACAGGTG 
GACACATATA GTCCCCAGGT GGGAACCCTC TTTCCTTGGG ACTCTGGCCC AGCCTTTGCT 
TTGCATCCTG AACCAGCCTT GCTTGGCTTG GGGAATCTGA CCAGAACTCC AGCAACCCTG 
GGGGCCAGGC CTGTAAGCAG GGCACTTGCA GTGACTCTGG CTCCTGCCAT GCCCACCAAG 
CCCCTGAGGA CTGTCCACCC AGATGTCTCT GAACACAGCT CTTCCCAGAC CCCACTGTCA 
CCTGCCAAAC AGTCGGCCAG GAAAACACCT TCCCCCTCCT CGTCAGCTTC CCTGGCTAAT 
TCCACCAGGG TTTATCGTCC AGCTGCTGCC CAGCCAAGAC AGATCACAAC TACTAGCCCC 
ACCAAACGTT CCCCCACCAA GCCTTCTGTT TCTCCCCTTT CAGTTACCCC CATGAAAAGT 
CCCCATGCAA CCCAGAAAAC AGGTGTCCCT TCATTCACAA AGCCTGTTCC ACCCACTCAG 
AAGCCAGCGC CCTTCACCTC CTACCTAGCA CCTTCCAAAG CCTCTAGTCC CACTGTGAGG 
CCTGTTCAGA AGACTTTCAT GACACCCCGA CCTCCAGTCC CCAGCCCCCA GCCCCTGCGC 
CCTACTACTG GCTTATCTAA GAAGTTCACT AACCCCACTG TTGCAAAATC TAAGTCCAAG 
ACGACCAGTT GGGCCAGCAA GCCGGTCTTG GCCCGCTCCA GTGTCCCTAA AACGCTTCAG 
CAAACTGTTT TGTCCCAGTC TCCTGTTTCC TATCTGGGCT CTCAGACACT GGCTCCAGCC 
CTCCCTCCTT TGGGTGTTGG AAATCCTAGA ACGATGCCTC CCACACGTGA CTCTGCTTTA 
ACTCCTGCTG GAAGCAAGAA ATTCACAGGA CGGGAAACCT CCAAGAAAAC CAGACAGAAG 
AGCAGCCCCC GGAAGCCCGA ACCTCTCAGC CCTGGGAAGT CAGCCAGGGA TGCCTCACCA 
AGAGACCTGA CAACCAAGCC TAGCCGGCCG TCCACCCCAG CCCTGGTCCT TGCCCCAGCT 
TACCTCCTGT CCTCCAGCCC CCAGCCCACC AGCAGCAGCT TCCCTTTCTT CCACCTGCTG 
GGCCCTACAC CTTTCCCGAT GCTGATGGGG CCTCCAGGCT CCAAGGGAGA CTGTGGCTTG 
CCGGGTCCCC CTGGGCTTCC AGGATTACCT GGATCTCCGG GCGCACGAGG TCCTCGGGGT 
CCTCCTGGGC CGTACGGGAA CCCAGGCCCA CCTGGCCCTC CAGGAGCCAA AGGACAGAAA 
GGGGACCCAG GACTCTCACC AGGACAGGCT CACGATGGAG CAAAGGGCAA CATGGGCTTG 
CCTGGGCTCT CCGGAAATCC AGGACCCTTG GGAAGGAAGG GACACAAGGG CCATCCTGGA 
GCAGCAGGGC ACCCTGGAGA ACAGGGGCAG CCAGGACCTG AGGGCAGTCC AGGGGCCAAA 
GGTTACCCTG GCAGGCAGGG GTTCCCTGGA CCTGTAGGAG ACCCTGGCCC CAAAGGCAGC 
AGGGGTTACA TTGGTCTTCC GGGGCTCTTT GGCCTGCCAG GGTCGGATGG AGAGCGGGGT 
CTCCCTGGCG TTCCTGGCAA GAGGGGCGAG ATGGGTAGGC CGGGCTTCCC TGGAGACTTT 
GGGGAAAGAG GCCCCCCTGG ACTTGATGGC AACCCTGGAG AAATTGGCCT GCCGGGGCCA 
CCAGGAGTGC TCGGGCTCAT TGGTGACACA GGAGCACTGG GCCCAGTTGG CTACCCAGGA 
CCGAAAGGCA TGAAGGGACT GATGGGCGGC GTGGGGGAGC CCGGACTGAA AGGTGATAAG 
GGTGAACAAG GGGTGCCAGG TGTGTCGGGA GATCCTGGCT TCCAAGGAGA CAAGGGGAGT 
CATGGCTTGC CAGGGCTCCC GGGTGGCCGG GGGAAGCCAG GGCCTCTGGG AAAAGCTGGA 
GACAAAGGAT CACTTGGGTT TCCTGGACCT CCTGGACCTG AGGGTTTCCC AGGAGACATT 
GGCCCTCCAG GGGACAATGG CCCTGAAGGC ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG 
CCTGGACCTC CTGGGCAGCT GGGGCCTGAG GGAGATGAAG GACCCATGGG GCCACCAGGA 
GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT 
GGCTCGAAGG GGGAACCAGG GGATCCTGGA CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT 
ATGGGATTCA TTGGTCTTGT CGGAGAGCCT GGAATTGTTG GGGAGAAGGG AGACCGTGGG 
GTGATGGGAC CCCCAGGTGC ACCTGGACCC AAGGGGTCGA TGGGCCATCC TGGAACACCC 
GGTGGTATTG GGAACCCTGG AGAGCCTGGA CCCTGGGGCC CTCCAGGATC TCGAGGCCTG 
CCAGGCATGA GGGGAGCCAA GGGGCACCGG GGCCCTCGAG GACCTGATGG ACCAGCTGGG 
GAGCAGGGAT CCAAGGGCCT GAAGGGTCGT GTAGGACCTC GGGGCAGACC TGGCCAGCCA 
GGACAGCAGG GTGCAGCTGG TGAGCGAGGC CACTCAGGTG CAAAAGGCTT CCTTGGCATC 
CCTGGTCCCT CAGGCCCTCC AGGTGCCAAG GGCCTCCCAG GAGAACCGGG CTCTCAGGGA 
CCTCAGGGGC CAGTTGGTCC TCCAGGAGAA ATGGGGCCCA AGGGACCGCC GGGTGCAGTG 
GGAGAGCCTG GCCTTCCTGG GGACTCCGGA ATGAAGGGTG ACCTCGGACC TCTGGGCCCC 
CCTGGGGAGC AAGGTCTTAT TGGGCAGCGG GGAGAGCCAG GCCTTGAGGG AGACCATGGC 
CCTGTGGGGC CAGATGGGCT GAAGGGCGAC AGGGGCGACC CAGGGCCCGA TGGCGAGCAT 
GGTGAAAAGG GCCAGGAAGG GCTGAAAGGA GAGGATGGGT CCCCTGGGCC CCCGGGCATC 
ACTGGCGTCC CGGGTCGTGA GGGGAAGCCG GGCAAGCAGG GTGAGAAAGG CCAGAGGGGA 
GCTAAGGGTG CCAAGGGCCA CCAAGGATAT CTGGGAGAGA TGGGCATCCC CGGAGAACCT 
GGGCCCCCTG GGACCCCAGG TCCTAAAGGG TCCCGGGGCA CCCTAGGACC AACGGGTGCT 
CCAGGACGGA TGGGAGCCCA AGGAGAACCA GGATTGGCCG GTTATAATGG ACACAAAGGC 
ATCACAGGAC CCCTCGGGCC TCCCGGGCCC AAAGGCGAGA AGGGGGACCA GGGCGAGGAT 
GGCAAGACCG AGGGGCCCCC TGGGCCACCA GGAGATCGGG GTCCTGTGGG TGATCGAGGA 
GACCGTGGGG AGCCAGGCGA CCCTGGATAC CCTGGTCAAG AGGGAGTTCA AGGCCTCCGT 
GGAGAACCGG GCCAGCAGGG ACAGCCTGGG CATCCAGGAC CCCGGGGGCG CCCAGGACCC 
AAAGGATCAA AAGGCGAAGA GGGCCCAAAG GGAAAGCCAG GCAAGGCTGG GCCATCAGGC 
CGGAGGGGGA CCCAGGGCCT TCAAGGACTG CCAGGCCCCC GAGGCGTGGT GGGGAGACAG 
GGCCCTGAGG GCACTGCTGG ATCTGATGGG ATTCCTGGCA GAGATGGTCG GCCAGGATAT 
CAGGGAGACC AGGGAAATGA TGGGGACCCT GGCCCTGTGG GCCCTGCTGG GAGAAGAGGA 
AATCCAGGTG TGGCTGGCTT GCCTGGAGCA CAGGGGCCTC CGGGATTCAA GGGTGAAAGT 
GGGTTACCTG GGCAACTGGG TCCCCCTGGA AAACGAGGGA CAGAAGGTGG AACAGGGCTT 
CCTGGGAACC AGGGGGAGCC AGGATCCAAA GGCCAGCCGG GTGACTCTGG CGAGATGGGC 
TTCCCAGGAG TGGCTGGCCT CTTTGGACCC AAGGGCCCCC CTGGAGACAT TGGCTTCAAA 
GGCATCCAAG GTCCTCGGGG GCCTCCTGGC TTGATGGGAA AGGAAGGTAT CATTGGCCCC 
CCCGGAATGC TGGGACCTTC TGGACTCCCG GGTCCCAAAG GTGACAGAGG CAGCCGAGGA 
GACTTGGGAC TGCAAGGCCC AAGGGGTCCT CCTGGTCCAA GGGGCCGGCC AGGTCCCCCG 
GGCCCTCCTT GGCATCCCAT CCAGTTTCAG CAAGATGACC TTGGAGCAGC TTTCCAGACA 
TGGATGGATG CTCAAGGAGC CGTCAGATCG GAGCAGGGGT ACAGCTATCC GGACCAGCTG 
GCGCTAGACC AGGGAGGGGA GATCTTCAAA ACCTTACACT ACCTCAGCAA CCTCATCCAG 
AGCATTAAGA CACCCTTGGG CACCAAGGAG AACCCAGCCC GGGTCTGCCG GGACCTCATG 
GACTGTGAGC AGAGGATGGC GGATGGTACC TACTGGGTGG ACCCCAACCT CGGCTGCTCC 
TCTGACACCA TTGAAGTCTC CTGCAACTTT ACACAGGGTG GGCAGACGTG CCTGAAGCCC 
ATCACGGCCT CCAAGGCCGA GTTTGCTGTG AGTCGGGTCC AGATGAATTT CTTGCACCTG 
CTGAGCTCTG AGGGGACACA GCATATCACA ATCCACTGTC TGAACATGAC GGTGTGGCAG 
GAGGGACCGG GACGCTCCTC TGCCAGACAG GCTGTGCGCT TCCGTGCCTG GAACGGACAG 
GTCTTCGAAG CTGGGGGTCA GTTCAGGCCA GAGGTGTCTA TGGATGGCTG CAAGGTCCAT 
GATGGCCGCT GGCATCAGAC ACTGTTCACC TTCCGGACCC AGGACCCCCA GCAGCTGCCC 
ATCGTCAGTG TGGACAATCT CCCGCCTGTC TCATCAGGGA AGCAGTACCG CCTGGAAGTT 
GGACCTGCGT GCTTCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006538098.1
CDS	504..6044
Translation	MGTGFARGARGTAASGPGGGFLFAWILVSFTCHLASTQGAPEDVDVLQRLGLSWTKAGGGRSPTPPGVIPFPSGFIFTQRAKLQAPTANVLPTTLGRELALVLSLCSHRVNHAFLFAIRSRKHKLQLGLQFLPGRTIIHLGPRQSVAFDLDVHDGRWHHLALELRGRTVTMVTACGQHRVPVPLPSRRDSMLDPQGSFLLGKVNPRAVQFEGALCQFSIHPVAQVAHNYCAHLRERCRQVDTYSPQVGTLFPWDSGPAFALHPEPALLGLGNLTRTPATLGARPVSRALAVTLAPAMPTKPLRTVHPDVSEHSSSQTPLSPAKQSARKTPSPSSSASLANSTRVYRPAAAQPRQITTTSPTKRSPTKPSVSPLSVTPMKSPHATQKTGVPSFTKPVPPTQKPAPFTSYLAPSKASSPTVRPVQKTFMTPRPPVPSPQPLRPTTGLSKKFTNPTVAKSKSKTTSWASKPVLARSSVPKTLQQTVLSQSPVSYLGSQTLAPALPPLGVGNPRTMPPTRDSALTPAGSKKFTGRETSKKTRQKSSPRKPEPLSPGKSARDASPRDLTTKPSRPSTPALVLAPAYLLSSSPQPTSSSFPFFHLLGPTPFPMLMGPPGSKGDCGLPGPPGLPGLPGSPGARGPRGPPGPYGNPGPPGPPGAKGQKGDPGLSPGQAHDGAKGNMGLPGLSGNPGPLGRKGHKGHPGAAGHPGEQGQPGPEGSPGAKGYPGRQGFPGPVGDPGPKGSRGYIGLPGLFGLPGSDGERGLPGVPGKRGEMGRPGFPGDFGERGPPGLDGNPGEIGLPGPPGVLGLIGDTGALGPVGYPGPKGMKGLMGGVGEPGLKGDKGEQGVPGVSGDPGFQGDKGSHGLPGLPGGRGKPGPLGKAGDKGSLGFPGPPGPEGFPGDIGPPGDNGPEGMKGKPGARGLPGPPGQLGPEGDEGPMGPPGVPGLEGQPGRKGFPGRPGLDGSKGEPGDPGRPGPVGEQGLMGFIGLVGEPGIVGEKGDRGVMGPPGAPGPKGSMGHPGTPGGIGNPGEPGPWGPPGSRGLPGMRGAKGHRGPRGPDGPAGEQGSKGLKGRVGPRGRPGQPGQQGAAGERGHSGAKGFLGIPGPSGPPGAKGLPGEPGSQGPQGPVGPPGEMGPKGPPGAVGEPGLPGDSGMKGDLGPLGPPGEQGLIGQRGEPGLEGDHGPVGPDGLKGDRGDPGPDGEHGEKGQEGLKGEDGSPGPPGITGVPGREGKPGKQGEKGQRGAKGAKGHQGYLGEMGIPGEPGPPGTPGPKGSRGTLGPTGAPGRMGAQGEPGLAGYNGHKGITGPLGPPGPKGEKGDQGEDGKTEGPPGPPGDRGPVGDRGDRGEPGDPGYPGQEGVQGLRGEPGQQGQPGHPGPRGRPGPKGSKGEEGPKGKPGKAGPSGRRGTQGLQGLPGPRGVVGRQGPEGTAGSDGIPGRDGRPGYQGDQGNDGDPGPVGPAGRRGNPGVAGLPGAQGPPGFKGESGLPGQLGPPGKRGTEGGTGLPGNQGEPGSKGQPGDSGEMGFPGVAGLFGPKGPPGDIGFKGIQGPRGPPGLMGKEGIIGPPGMLGPSGLPGPKGDRGSRGDLGLQGPRGPPGPRGRPGPPGPPWHPIQFQQDDLGAAFQTWMDAQGAVRSEQGYSYPDQLALDQGGEIFKTLHYLSNLIQSIKTPLGTKENPARVCRDLMDCEQRMADGTYWVDPNLGCSSDTIEVSCNFTQGGQTCLKPITASKAEFAVSRVQMNFLHLLSSEGTQHITIHCLNMTVWQEGPGRSSARQAVRFRAWNGQVFEAGGQFRPEVSMDGCKVHDGRWHQTLFTFRTQDPQQLPIVSVDNLPPVSSGKQYRLEVGPACFL

Target ORF information:

RefSeq Version	XM_006538035.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006538035.4

ORF Insert Sequence:

ATGGGCACGG GATTCGCGCG GGGGGCCCGA GGCACAGCGG CGTCAGGACC CGGGGGGGGG 
TTTCTCTTCG CCTGGATCTT GGTCTCATTT ACCTGTCACC TGGCCTCCAC CCAAGGAGCT 
CCTGAAGATG TGGATGTCCT CCAGCGGCTG GGCCTCAGCT GGACGAAGGC CGGGGGTGGC 
CGGAGTCCTA CACCCCCTGG TGTCATTCCT TTCCCATCTG GCTTCATCTT CACACAGCGG 
GCCAAGCTGC AGGCCCCCAC AGCCAATGTT CTTCCCACCA CCCTGGGGCG GGAGCTGGCA 
TTGGTGTTGA GCCTCTGTTC ACATCGTGTG AACCATGCCT TCCTCTTTGC CATCCGCAGC 
AGGAAACACA AGCTGCAACT GGGTCTGCAG TTCCTCCCCG GCAGGACGAT CATCCACCTG 
GGGCCTCGGC AATCAGTGGC CTTCGACTTG GATGTGCATG ATGGGCGCTG GCACCACCTG 
GCCCTAGAGT TGCGTGGCCG CACAGTCACA ATGGTGACAG CCTGTGGGCA GCACAGGGTA 
CCTGTCCCAC TGCCTTCCCG TAGGGACTCC ATGCTTGACC CCCAGGGCTC CTTTCTCTTG 
GGGAAGGTGA ACCCCCGTGC TGTCCAGTTT GAAGGTGCAC TCTGTCAGTT CAGCATCCAC 
CCTGTGGCGC AGGTCGCTCA CAATTACTGT GCCCACCTGA GAGAGCGGTG CCGACAGGTG 
GACACATATA GTCCCCAGGT GGGAACCCTC TTTCCTTGGG ACTCTGGCCC AGCCTTTGCT 
TTGCATCCTG AACCAGCCTT GCTTGGCTTG GGGAATCTGA CCAGAACTCC AGCAACCCTG 
GGGGCCAGGC CTGTAAGCAG GGCACTTGCA GTGACTCTGG CTCCTGCCAT GCCCACCAAG 
CCCCTGAGGA CTGTCCACCC AGATGTCTCT GAACACAGCT CTTCCCAGAC CCCACTGTCA 
CCTGCCAAAC AGTCGGCCAG GAAAACACCT TCCCCCTCCT CGTCAGCTTC CCTGGCTAAT 
TCCACCAGGG TTTATCGTCC AGCTGCTGCC CAGCCAAGAC AGATCACAAC TACTAGCCCC 
ACCAAACGTT CCCCCACCAA GCCTTCTGTT TCTCCCCTTT CAGTTACCCC CATGAAAAGT 
CCCCATGCAA CCCAGAAAAC AGGTGTCCCT TCATTCACAA AGCCTGTTCC ACCCACTCAG 
AAGCCAGCGC CCTTCACCTC CTACCTAGCA CCTTCCAAAG CCTCTAGTCC CACTGTGAGG 
CCTGTTCAGA AGACTTTCAT GACACCCCGA CCTCCAGTCC CCAGCCCCCA GCCCCTGCGC 
CCTACTACTG GCTTATCTAA GAAGTTCACT AACCCCACTG TTGCAAAATC TAAGTCCAAG 
ACGACCAGTT GGGCCAGCAA GCCGGTCTTG GCCCGCTCCA GTGTCCCTAA AACGCTTCAG 
CAAACTGTTT TGTCCCAGTC TCCTGTTTCC TATCTGGGCT CTCAGACACT GGCTCCAGCC 
CTCCCTCCTT TGGGTGTTGG AAATCCTAGA ACGATGCCTC CCACACGTGA CTCTGCTTTA 
ACTCCTGCTG GAAGCAAGAA ATTCACAGGA CGGGAAACCT CCAAGAAAAC CAGACAGAAG 
AGCAGCCCCC GGAAGCCCGA ACCTCTCAGC CCTGGGAAGT CAGCCAGGGA TGCCTCACCA 
AGAGACCTGA CAACCAAGCC TAGCCGGCCG TCCACCCCAG CCCTGGTCCT TGCCCCAGCT 
TACCTCCTGT CCTCCAGCCC CCAGCCCACC AGCAGCAGCT TCCCTTTCTT CCACCTGCTG 
GGCCCTACAC CTTTCCCGAT GCTGATGGGG CCTCCAGGCT CCAAGGGAGA CTGTGGCTTG 
CCGGGTCCCC CTGGGCTTCC AGGATTACCT GGATCTCCGG GCGCACGAGG TCCTCGGGGT 
CCTCCTGGGC CGTACGGGAA CCCAGGCCCA CCTGGCCCTC CAGGAGCCAA AGGACAGAAA 
GGGGACCCAG GACTCTCACC AGGACAGGCT CACGATGGAG CAAAGGGCAA CATGGGCTTG 
CCTGGGCTCT CCGGAAATCC AGGACCCTTG GGAAGGAAGG GACACAAGGG CCATCCTGGA 
GCAGCAGGGC ACCCTGGAGA ACAGGGGCAG CCAGGACCTG AGGGCAGTCC AGGGGCCAAA 
GGTTACCCTG GCAGGCAGGG GTTCCCTGGA CCTGTAGGAG ACCCTGGCCC CAAAGGCAGC 
AGGGGTTACA TTGGTCTTCC GGGGCTCTTT GGCCTGCCAG GGTCGGATGG AGAGCGGGGT 
CTCCCTGGCG TTCCTGGCAA GAGGGGCGAG ATGGGTAGGC CGGGCTTCCC TGGAGACTTT 
GGGGAAAGAG GCCCCCCTGG ACTTGATGGC AACCCTGGAG AAATTGGCCT GCCGGGGCCA 
CCAGGAGTGC TCGGGCTCAT TGGTGACACA GGAGCACTGG GCCCAGTTGG CTACCCAGGA 
CCGAAAGGCA TGAAGGGACT GATGGGCGGC GTGGGGGAGC CCGGACTGAA AGGTGATAAG 
GGTGAACAAG GGGTGCCAGG TGTGTCGGGA GATCCTGGCT TCCAAGGAGA CAAGGGGAGT 
CATGGCTTGC CAGGGCTCCC GGGTGGCCGG GGGAAGCCAG GGCCTCTGGG AAAAGCTGGA 
GACAAAGGAT CACTTGGGTT TCCTGGACCT CCTGGACCTG AGGGTTTCCC AGGAGACATT 
GGCCCTCCAG GGGACAATGG CCCTGAAGGC ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG 
CCTGGACCTC CTGGGCAGCT GGGGCCTGAG GGAGATGAAG GACCCATGGG GCCACCAGGA 
GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT 
GGCTCGAAGG GGGAACCAGG GGATCCTGGA CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT 
ATGGGATTCA TTGGTCTTGT CGGAGAGCCT GGAATTGTTG GGGAGAAGGG AGACCGTGGG 
GTGATGGGAC CCCCAGGTGC ACCTGGACCC AAGGGGTCGA TGGGCCATCC TGGAACACCC 
GGTGGTATTG GGAACCCTGG AGAGCCTGGA CCCTGGGGCC CTCCAGGATC TCGAGGCCTG 
CCAGGCATGA GGGGAGCCAA GGGGCACCGG GGCCCTCGAG GACCTGATGG ACCAGCTGGG 
GAGCAGGGAT CCAAGGGCCT GAAGGGTCGT GTAGGACCTC GGGGCAGACC TGGCCAGCCA 
GGACAGCAGG GTGCAGCTGG TGAGCGAGGC CACTCAGGTG CAAAAGGCTT CCTTGGCATC 
CCTGGTCCCT CAGGCCCTCC AGGTGCCAAG GGCCTCCCAG GAGAACCGGG CTCTCAGGGA 
CCTCAGGGGC CAGTTGGTCC TCCAGGAGAA ATGGGGCCCA AGGGACCGCC GGGTGCAGTG 
GGAGAGCCTG GCCTTCCTGG GGACTCCGGA ATGAAGGGTG ACCTCGGACC TCTGGGCCCC 
CCTGGGGAGC AAGGTCTTAT TGGGCAGCGG GGAGAGCCAG GCCTTGAGGG AGACCATGGC 
CCTGTGGGGC CAGATGGGCT GAAGGGCGAC AGGGGCGACC CAGGGCCCGA TGGCGAGCAT 
GGTGAAAAGG GCCAGGAAGG GCTGAAAGGA GAGGATGGGT CCCCTGGGCC CCCGGGCATC 
ACTGGCGTCC CGGGTCGTGA GGGGAAGCCG GGCAAGCAGG GTGAGAAAGG CCAGAGGGGA 
GCTAAGGGTG CCAAGGGCCA CCAAGGATAT CTGGGAGAGA TGGGCATCCC CGGAGAACCT 
GGGCCCCCTG GGACCCCAGG TCCTAAAGGG TCCCGGGGCA CCCTAGGACC AACGGGTGCT 
CCAGGACGGA TGGGAGCCCA AGGAGAACCA GGATTGGCCG GTTATAATGG ACACAAAGGC 
ATCACAGGAC CCCTCGGGCC TCCCGGGCCC AAAGGCGAGA AGGGGGACCA GGGCGAGGAT 
GGCAAGACCG AGGGGCCCCC TGGGCCACCA GGAGATCGGG GTCCTGTGGG TGATCGAGGA 
GACCGTGGGG AGCCAGGCGA CCCTGGATAC CCTGGTCAAG AGGGAGTTCA AGGCCTCCGT 
GGAGAACCGG GCCAGCAGGG ACAGCCTGGG CATCCAGGAC CCCGGGGGCG CCCAGGACCC 
AAAGGATCAA AAGGCGAAGA GGGCCCAAAG GGAAAGCCAG GCAAGGCTGG GCCATCAGGC 
CGGAGGGGGA CCCAGGGCCT TCAAGGACTG CCAGGCCCCC GAGGCGTGGT GGGGAGACAG 
GGCCCTGAGG GCACTGCTGG ATCTGATGGG ATTCCTGGCA GAGATGGTCG GCCAGGATAT 
CAGGGAGACC AGGGAAATGA TGGGGACCCT GGCCCTGTGG GCCCTGCTGG GAGAAGAGGA 
AATCCAGGTG TGGCTGGCTT GCCTGGAGCA CAGGGGCCTC CGGGATTCAA GGGTGAAAGT 
GGGTTACCTG GGCAACTGGG TCCCCCTGGA AAACGAGGGA CAGAAGGTGG AACAGGGCTT 
CCTGGGAACC AGGGGGAGCC AGGATCCAAA GGCCAGCCGG GTGACTCTGG CGAGATGGGC 
TTCCCAGGAG TGGCTGGCCT CTTTGGACCC AAGGGCCCCC CTGGAGACAT TGGCTTCAAA 
GGCATCCAAG GTCCTCGGGG GCCTCCTGGC TTGATGGGAA AGGAAGGTAT CATTGGCCCC 
CCCGGAATGC TGGGACCTTC TGGACTCCCG GGTCCCAAAG GTGACAGAGG CAGCCGAGGA 
GACTTGGGAC TGCAAGGCCC AAGGGGTCCT CCTGGTCCAA GGGGCCGGCC AGGTCCCCCG 
GGCCCTCCTT GGCATCCCAT CCAGTTTCAG CAAGATGACC TTGGAGCAGC TTTCCAGACA 
TGGATGGATG CTCAAGGAGC CGTCAGATCG GAGCAGGGGT ACAGCTATCC GGACCAGCTG 
GCGCTAGACC AGGGAGGGGA GATCTTCAAA ACCTTACACT ACCTCAGCAA CCTCATCCAG 
AGCATTAAGA CACCCTTGGG CACCAAGGAG AACCCAGCCC GGGTCTGCCG GGACCTCATG 
GACTGTGAGC AGAGGATGGC GGATGGTACC TACTGGGTGG ACCCCAACCT CGGCTGCTCC 
TCTGACACCA TTGAAGTCTC CTGCAACTTT ACACAGGGTG GGCAGACGTG CCTGAAGCCC 
ATCACGGCCT CCAAGGCCGA GTTTGCTGTG AGTCGGGTCC AGATGAATTT CTTGCACCTG 
CTGAGCTCTG AGGGGACACA GCATATCACA ATCCACTGTC TGAACATGAC GGTGTGGCAG 
GAGGGACCGG GACGCTCCTC TGCCAGACAG GCTGTGCGCT TCCGTGCCTG GAACGGACAG 
GTCTTCGAAG CTGGGGGTCA GTTCAGGCCA GAGGTGTCTA TGGATGGCTG CAAGGTCCAT 
GATGGCCGCT GGCATCAGAC ACTGTTCACC TTCCGGACCC AGGACCCCCA GCAGCTGCCC 
ATCGTCAGTG TGGACAATCT CCCGCCTGTC TCATCAGGGA AGCAGTACCG CCTGGAAGTT 
GGACCTGCGT GCTTCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col27a1 ( XM_017320282.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175771.1 Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X1, mRNA.

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CloneID	OMu81036
Clone ID Related Accession (Same CDS sequence)	XM_017320282.1
Accession Version	XM_017320282.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5616bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	collagen alpha-1(XXVII) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCACGG GATTCGCGCG GGGGGCCCGA GGCACAGCGG CGTCAGGACC CGGGGGGGGG 
TTTCTCTTCG CCTGGATCTT GGTCTCATTT ACCTGTCACC TGGCCTCCAC CCAAGGAGCT 
CCTGAAGATG TGGATGTCCT CCAGCGGCTG GGCCTCAGCT GGACGAAGGC CGGGGGTGGC 
CGGAGTCCTA CACCCCCTGG TGTCATTCCT TTCCCATCTG GCTTCATCTT CACACAGCGG 
GCCAAGCTGC AGGCCCCCAC AGCCAATGTT CTTCCCACCA CCCTGGGGCG GGAGCTGGCA 
TTGGTGTTGA GCCTCTGTTC ACATCGTGTG AACCATGCCT TCCTCTTTGC CATCCGCAGC 
AGGAAACACA AGCTGCAACT GGGTCTGCAG TTCCTCCCCG GCAGGACGAT CATCCACCTG 
GGGCCTCGGC AATCAGTGGC CTTCGACTTG GATGTGCATG ATGGGCGCTG GCACCACCTG 
GCCCTAGAGT TGCGTGGCCG CACAGTCACA ATGGTGACAG CCTGTGGGCA GCACAGGGTA 
CCTGTCCCAC TGCCTTCCCG TAGGGACTCC ATGCTTGACC CCCAGGGCTC CTTTCTCTTG 
GGGAAGGTGA ACCCCCGTGC TGTCCAGTTT GAAGGTGCAC TCTGTCAGTT CAGCATCCAC 
CCTGTGGCGC AGGTCGCTCA CAATTACTGT GCCCACCTGA GAGAGCGGTG CCGACAGGTG 
GACACATATA GTCCCCAGGT GGGAACCCTC TTTCCTTGGG ACTCTGGCCC AGCCTTTGCT 
TTGCATCCTG AACCAGCCTT GCTTGGCTTG GGGAATCTGA CCAGAACTCC AGCAACCCTG 
GGGGCCAGGC CTGTAAGCAG GGCACTTGCA GTGACTCTGG CTCCTGCCAT GCCCACCAAG 
CCCCTGAGGA CTGTCCACCC AGATGTCTCT GAACACAGCT CTTCCCAGAC CCCACTGTCA 
CCTGCCAAAC AGTCGGCCAG GAAAACACCT TCCCCCTCCT CGTCAGCTTC CCTGGCTAAT 
TCCACCAGGG TTTATCGTCC AGCTGCTGCC CAGCCAAGAC AGATCACAAC TACTAGCCCC 
ACCAAACGTT CCCCCACCAA GCCTTCTGTT TCTCCCCTTT CAGTTACCCC CATGAAAAGT 
CCCCATGCAA CCCAGAAAAC AGGTGTCCCT TCATTCACAA AGCCTGTTCC ACCCACTCAG 
AAGCCAGCGC CCTTCACCTC CTACCTAGCA CCTTCCAAAG CCTCTAGTCC CACTGTGAGG 
CCTGTTCAGA AGACTTTCAT GACACCCCGA CCTCCAGTCC CCAGCCCCCA GCCCCTGCGC 
CCTACTACTG GCTTATCTAA GAAGTTCACT AACCCCACTG TTGCAAAATC TAAGTCCAAG 
ACGACCAGTT GGGCCAGCAA GCCGGTCTTG GCCCGCTCCA GTGTCCCTAA AACGCTTCAG 
CAAACTGTTT TGTCCCAGTC TCCTGTTTCC TATCTGGGCT CTCAGACACT GGCTCCAGCC 
CTCCCTCCTT TGGGTGTTGG AAATCCTAGA ACGATGCCTC CCACACGTGA CTCTGCTTTA 
ACTCCTGCTG GAAGCAAGAA ATTCACAGGA CGGGAAACCT CCAAGAAAAC CAGACAGAAG 
AGCAGCCCCC GGAAGCCCGA ACCTCTCAGC CCTGGGAAGT CAGCCAGGGA TGCCTCACCA 
AGAGACCTGA CAACCAAGCC TAGCCGGCCG TCCACCCCAG CCCTGGTCCT TGCCCCAGCT 
TACCTCCTGT CCTCCAGCCC CCAGCCCACC AGCAGCAGCT TCCCTTTCTT CCACCTGCTG 
GGCCCTACAC CTTTCCCGAT GCTGATGGGG CCTCCAGGCT CCAAGGGAGA CTGTGGCTTG 
CCGGGTCCCC CTGGGCTTCC AGGATTACCT GGATCTCCGG GCGCACGAGG TCCTCGGGGT 
CCTCCTGGGC CGTACGGGAA CCCAGGCCCA CCTGGCCCTC CAGGAGCCAA AGGACAGAAA 
GGGGACCCAG GACTCTCACC AGGACAGGCT CACGATGGAG CAAAGGGCAA CATGGGCTTG 
CCTGGGCTCT CCGGAAATCC AGGACCCTTG GGAAGGAAGG GACACAAGGG CCATCCTGGA 
GCAGCAGGGC ACCCTGGAGA ACAGGGGCAG CCAGGACCTG AGGGCAGTCC AGGGGCCAAA 
GGTTACCCTG GCAGGCAGGG GTTCCCTGGA CCTGTAGGAG ACCCTGGCCC CAAAGGCAGC 
AGGGGTTACA TTGGTCTTCC GGGGCTCTTT GGCCTGCCAG GGTCGGATGG AGAGCGGGGT 
CTCCCTGGCG TTCCTGGCAA GAGGGGCGAG ATGGGTAGGC CGGGCTTCCC TGGAGACTTT 
GGGGAAAGAG GCCCCCCTGG ACTTGATGGC AACCCTGGAG AAATTGGCCT GCCGGGGCCA 
CCAGGAGTGC TCGGGCTCAT TGGTGACACA GGAGCACTGG GCCCAGTTGG CTACCCAGGA 
CCGAAAGGCA TGAAGGGACT GATGGGCGGC GTGGGGGAGC CCGGACTGAA AGGTGATAAG 
GGTGAACAAG GGGTGCCAGG TGTGTCGGGA GATCCTGGCT TCCAAGGAGA CAAGGGGAGT 
CATGGCTTGC CAGGGCTCCC GGGTGGCCGG GGGAAGCCAG GGCCTCTGGG AAAAGCTGGA 
GACAAAGGAT CACTTGGGTT TCCTGGACCT CCTGGACCTG AGGGTTTCCC AGGAGACATT 
GGCCCTCCAG GGGACAATGG CCCTGAAGGC ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG 
CCTGGACCTC CTGGGCAGCT GGGGCCTGAG GGAGATGAAG GACCCATGGG GCCACCAGGA 
GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT 
GGCTCGAAGG GGGAACCAGG GGATCCTGGA CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT 
ATGGGATTCA TTGGTCTTGT CGGAGAGCCT GGAATTGTTG GGGAGAAGGG AGACCGTGGG 
GTGATGGGAC CCCCAGGTGC ACCTGGACCC AAGGGGTCGA TGGGCCATCC TGGAACACCC 
GGTGGTATTG GGAACCCTGG AGAGCCTGGA CCCTGGGGCC CTCCAGGATC TCGAGGCCTG 
CCAGGCATGA GGGGAGCCAA GGGGCACCGG GGCCCTCGAG GACCTGATGG ACCAGCTGGG 
GAGCAGGGAT CCAAGGGCCT GAAGGGTCGT GTAGGACCTC GGGGCAGACC TGGCCAGCCA 
GGACAGCAGG GTGCAGCTGG TGAGCGAGGC CACTCAGGTG CAAAAGGCTT CCTTGGCATC 
CCTGGTCCCT CAGGCCCTCC AGGTGCCAAG GGCCTCCCAG GAGAACCGGG CTCTCAGGGA 
CCTCAGGGGC CAGTTGGTCC TCCAGGAGAA ATGGGGCCCA AGGGACCGCC GGGTGCAGTG 
GGAGAGCCTG GCCTTCCTGG GGACTCCGGA ATGAAGGGTG ACCTCGGACC TCTGGGCCCC 
CCTGGGGAGC AAGGTCTTAT TGGGCAGCGG GGAGAGCCAG GCCTTGAGGG AGACCATGGC 
CCTGTGGGGC CAGATGGGCT GAAGGGCGAC AGGGGCGACC CAGGGCCCGA TGGCGAGCAT 
GGTGAAAAGG GCCAGGAAGG GCTGAAAGGA GAGGATGGGT CCCCTGGGCC CCCGGGCATC 
ACTGGCGTCC CGGGTCGTGA GGGGAAGCCG GGCAAGCAGG GTGAGAAAGG CCAGAGGGGA 
GCTAAGGGTG CCAAGGGCCA CCAAGGATAT CTGGGAGAGA TGGGCATCCC CGGAGAACCT 
GGGCCCCCTG GGACCCCAGG TCCTAAAGGG TCCCGGGGCA CCCTAGGACC AACGGGTGCT 
CCAGGACGGA TGGGAGCCCA AGGAGAACCA GGATTGGCCG GTTATAATGG ACACAAAGGC 
ATCACAGGAC CCCTCGGGCC TCCCGGGCCC AAAGGCGAGA AGGGGGACCA GGGCGAGGAT 
GGCAAGACCG AGGGGCCCCC TGGGCCACCA GGAGATCGGG GTCCTGTGGG TGATCGAGGA 
GACCGTGGGG AGCCAGGCGA CCCTGGATAC CCTGGTCAAG AGGGAGTTCA AGGCCTCCGT 
GGAGAACCGG GCCAGCAGGG ACAGCCTGGG CATCCAGGAC CCCGGGGGCG CCCAGGACCC 
AAAGGATCAA AAGGCGAAGA GGGCCCAAAG GGAAAGCCAG GCAAGGCTGG GCCATCAGGC 
CGGAGGGGGA CCCAGGTGAG TTGCCATAGC TTCTCTGTCA CCTTCTATGT TCTCCTGAGA 
AGGTTTAACC AGCTTCTTCT GTACCTCCAG GGCCTTCAAG GACTGCCAGG CCCCCGAGGC 
GTGGTGGGGA GACAGGGCCC TGAGGGCACT GCTGGATCTG ATGGGATTCC TGGCAGAGAT 
GGTCGGCCAG GATATCAGGG AGACCAGGGA AATGATGGGG ACCCTGGCCC TGTGGGCCCT 
GCTGGGAGAA GAGGAAATCC AGGTGTGGCT GGCTTGCCTG GAGCACAGGG GCCTCCGGGA 
TTCAAGGGTG AAAGTGGGTT ACCTGGGCAA CTGGGTCCCC CTGGAAAACG AGGGACAGAA 
GGTGGAACAG GGCTTCCTGG GAACCAGGGG GAGCCAGGAT CCAAAGGCCA GCCGGGTGAC 
TCTGGCGAGA TGGGCTTCCC AGGAGTGGCT GGCCTCTTTG GACCCAAGGG CCCCCCTGGA 
GACATTGGCT TCAAAGGCAT CCAAGGTCCT CGGGGGCCTC CTGGCTTGAT GGGAAAGGAA 
GGTATCATTG GCCCCCCCGG AATGCTGGGA CCTTCTGGAC TCCCGGGTCC CAAAGGTGAC 
AGAGGCAGCC GAGGAGACTT GGGACTGCAA GGCCCAAGGG GTCCTCCTGG TCCAAGGGGC 
CGGCCAGGTC CCCCGGGCCC TCCTTGGCAT CCCATCCAGT TTCAGCAAGA TGACCTTGGA 
GCAGCTTTCC AGACATGGAT GGATGCTCAA GGAGCCGTCA GATCGGAGCA GGGGTACAGC 
TATCCGGACC AGCTGGCGCT AGACCAGGGA GGGGAGATCT TCAAAACCTT ACACTACCTC 
AGCAACCTCA TCCAGAGCAT TAAGACACCC TTGGGCACCA AGGAGAACCC AGCCCGGGTC 
TGCCGGGACC TCATGGACTG TGAGCAGAGG ATGGCGGATG GTACCTACTG GGTGGACCCC 
AACCTCGGCT GCTCCTCTGA CACCATTGAA GTCTCCTGCA ACTTTACACA GGGTGGGCAG 
ACGTGCCTGA AGCCCATCAC GGCCTCCAAG GCCGAGTTTG CTGTGAGTCG GGTCCAGATG 
AATTTCTTGC ACCTGCTGAG CTCTGAGGGG ACACAGCATA TCACAATCCA CTGTCTGAAC 
ATGACGGTGT GGCAGGAGGG ACCGGGACGC TCCTCTGCCA GACAGGCTGT GCGCTTCCGT 
GCCTGGAACG GACAGGTCTT CGAAGCTGGG GGTCAGTTCA GGCCAGAGGT GTCTATGGAT 
GGCTGCAAGG TCCATGATGG CCGCTGGCAT CAGACACTGT TCACCTTCCG GACCCAGGAC 
CCCCAGCAGC TGCCCATCGT CAGTGTGGAC AATCTCCCGC CTGTCTCATC AGGGAAGCAG 
TACCGCCTGG AAGTTGGACC TGCGTGCTTC CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175771.1
CDS	507..6122
Translation	MGTGFARGARGTAASGPGGGFLFAWILVSFTCHLASTQGAPEDVDVLQRLGLSWTKAGGGRSPTPPGVIPFPSGFIFTQRAKLQAPTANVLPTTLGRELALVLSLCSHRVNHAFLFAIRSRKHKLQLGLQFLPGRTIIHLGPRQSVAFDLDVHDGRWHHLALELRGRTVTMVTACGQHRVPVPLPSRRDSMLDPQGSFLLGKVNPRAVQFEGALCQFSIHPVAQVAHNYCAHLRERCRQVDTYSPQVGTLFPWDSGPAFALHPEPALLGLGNLTRTPATLGARPVSRALAVTLAPAMPTKPLRTVHPDVSEHSSSQTPLSPAKQSARKTPSPSSSASLANSTRVYRPAAAQPRQITTTSPTKRSPTKPSVSPLSVTPMKSPHATQKTGVPSFTKPVPPTQKPAPFTSYLAPSKASSPTVRPVQKTFMTPRPPVPSPQPLRPTTGLSKKFTNPTVAKSKSKTTSWASKPVLARSSVPKTLQQTVLSQSPVSYLGSQTLAPALPPLGVGNPRTMPPTRDSALTPAGSKKFTGRETSKKTRQKSSPRKPEPLSPGKSARDASPRDLTTKPSRPSTPALVLAPAYLLSSSPQPTSSSFPFFHLLGPTPFPMLMGPPGSKGDCGLPGPPGLPGLPGSPGARGPRGPPGPYGNPGPPGPPGAKGQKGDPGLSPGQAHDGAKGNMGLPGLSGNPGPLGRKGHKGHPGAAGHPGEQGQPGPEGSPGAKGYPGRQGFPGPVGDPGPKGSRGYIGLPGLFGLPGSDGERGLPGVPGKRGEMGRPGFPGDFGERGPPGLDGNPGEIGLPGPPGVLGLIGDTGALGPVGYPGPKGMKGLMGGVGEPGLKGDKGEQGVPGVSGDPGFQGDKGSHGLPGLPGGRGKPGPLGKAGDKGSLGFPGPPGPEGFPGDIGPPGDNGPEGMKGKPGARGLPGPPGQLGPEGDEGPMGPPGVPGLEGQPGRKGFPGRPGLDGSKGEPGDPGRPGPVGEQGLMGFIGLVGEPGIVGEKGDRGVMGPPGAPGPKGSMGHPGTPGGIGNPGEPGPWGPPGSRGLPGMRGAKGHRGPRGPDGPAGEQGSKGLKGRVGPRGRPGQPGQQGAAGERGHSGAKGFLGIPGPSGPPGAKGLPGEPGSQGPQGPVGPPGEMGPKGPPGAVGEPGLPGDSGMKGDLGPLGPPGEQGLIGQRGEPGLEGDHGPVGPDGLKGDRGDPGPDGEHGEKGQEGLKGEDGSPGPPGITGVPGREGKPGKQGEKGQRGAKGAKGHQGYLGEMGIPGEPGPPGTPGPKGSRGTLGPTGAPGRMGAQGEPGLAGYNGHKGITGPLGPPGPKGEKGDQGEDGKTEGPPGPPGDRGPVGDRGDRGEPGDPGYPGQEGVQGLRGEPGQQGQPGHPGPRGRPGPKGSKGEEGPKGKPGKAGPSGRRGTQVSCHSFSVTFYVLLRRFNQLLLYLQGLQGLPGPRGVVGRQGPEGTAGSDGIPGRDGRPGYQGDQGNDGDPGPVGPAGRRGNPGVAGLPGAQGPPGFKGESGLPGQLGPPGKRGTEGGTGLPGNQGEPGSKGQPGDSGEMGFPGVAGLFGPKGPPGDIGFKGIQGPRGPPGLMGKEGIIGPPGMLGPSGLPGPKGDRGSRGDLGLQGPRGPPGPRGRPGPPGPPWHPIQFQQDDLGAAFQTWMDAQGAVRSEQGYSYPDQLALDQGGEIFKTLHYLSNLIQSIKTPLGTKENPARVCRDLMDCEQRMADGTYWVDPNLGCSSDTIEVSCNFTQGGQTCLKPITASKAEFAVSRVQMNFLHLLSSEGTQHITIHCLNMTVWQEGPGRSSARQAVRFRAWNGQVFEAGGQFRPEVSMDGCKVHDGRWHQTLFTFRTQDPQQLPIVSVDNLPPVSSGKQYRLEVGPACFL

Target ORF information:

RefSeq Version	XM_017320282.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320282.1

ORF Insert Sequence:

ATGGGCACGG GATTCGCGCG GGGGGCCCGA GGCACAGCGG CGTCAGGACC CGGGGGGGGG 
TTTCTCTTCG CCTGGATCTT GGTCTCATTT ACCTGTCACC TGGCCTCCAC CCAAGGAGCT 
CCTGAAGATG TGGATGTCCT CCAGCGGCTG GGCCTCAGCT GGACGAAGGC CGGGGGTGGC 
CGGAGTCCTA CACCCCCTGG TGTCATTCCT TTCCCATCTG GCTTCATCTT CACACAGCGG 
GCCAAGCTGC AGGCCCCCAC AGCCAATGTT CTTCCCACCA CCCTGGGGCG GGAGCTGGCA 
TTGGTGTTGA GCCTCTGTTC ACATCGTGTG AACCATGCCT TCCTCTTTGC CATCCGCAGC 
AGGAAACACA AGCTGCAACT GGGTCTGCAG TTCCTCCCCG GCAGGACGAT CATCCACCTG 
GGGCCTCGGC AATCAGTGGC CTTCGACTTG GATGTGCATG ATGGGCGCTG GCACCACCTG 
GCCCTAGAGT TGCGTGGCCG CACAGTCACA ATGGTGACAG CCTGTGGGCA GCACAGGGTA 
CCTGTCCCAC TGCCTTCCCG TAGGGACTCC ATGCTTGACC CCCAGGGCTC CTTTCTCTTG 
GGGAAGGTGA ACCCCCGTGC TGTCCAGTTT GAAGGTGCAC TCTGTCAGTT CAGCATCCAC 
CCTGTGGCGC AGGTCGCTCA CAATTACTGT GCCCACCTGA GAGAGCGGTG CCGACAGGTG 
GACACATATA GTCCCCAGGT GGGAACCCTC TTTCCTTGGG ACTCTGGCCC AGCCTTTGCT 
TTGCATCCTG AACCAGCCTT GCTTGGCTTG GGGAATCTGA CCAGAACTCC AGCAACCCTG 
GGGGCCAGGC CTGTAAGCAG GGCACTTGCA GTGACTCTGG CTCCTGCCAT GCCCACCAAG 
CCCCTGAGGA CTGTCCACCC AGATGTCTCT GAACACAGCT CTTCCCAGAC CCCACTGTCA 
CCTGCCAAAC AGTCGGCCAG GAAAACACCT TCCCCCTCCT CGTCAGCTTC CCTGGCTAAT 
TCCACCAGGG TTTATCGTCC AGCTGCTGCC CAGCCAAGAC AGATCACAAC TACTAGCCCC 
ACCAAACGTT CCCCCACCAA GCCTTCTGTT TCTCCCCTTT CAGTTACCCC CATGAAAAGT 
CCCCATGCAA CCCAGAAAAC AGGTGTCCCT TCATTCACAA AGCCTGTTCC ACCCACTCAG 
AAGCCAGCGC CCTTCACCTC CTACCTAGCA CCTTCCAAAG CCTCTAGTCC CACTGTGAGG 
CCTGTTCAGA AGACTTTCAT GACACCCCGA CCTCCAGTCC CCAGCCCCCA GCCCCTGCGC 
CCTACTACTG GCTTATCTAA GAAGTTCACT AACCCCACTG TTGCAAAATC TAAGTCCAAG 
ACGACCAGTT GGGCCAGCAA GCCGGTCTTG GCCCGCTCCA GTGTCCCTAA AACGCTTCAG 
CAAACTGTTT TGTCCCAGTC TCCTGTTTCC TATCTGGGCT CTCAGACACT GGCTCCAGCC 
CTCCCTCCTT TGGGTGTTGG AAATCCTAGA ACGATGCCTC CCACACGTGA CTCTGCTTTA 
ACTCCTGCTG GAAGCAAGAA ATTCACAGGA CGGGAAACCT CCAAGAAAAC CAGACAGAAG 
AGCAGCCCCC GGAAGCCCGA ACCTCTCAGC CCTGGGAAGT CAGCCAGGGA TGCCTCACCA 
AGAGACCTGA CAACCAAGCC TAGCCGGCCG TCCACCCCAG CCCTGGTCCT TGCCCCAGCT 
TACCTCCTGT CCTCCAGCCC CCAGCCCACC AGCAGCAGCT TCCCTTTCTT CCACCTGCTG 
GGCCCTACAC CTTTCCCGAT GCTGATGGGG CCTCCAGGCT CCAAGGGAGA CTGTGGCTTG 
CCGGGTCCCC CTGGGCTTCC AGGATTACCT GGATCTCCGG GCGCACGAGG TCCTCGGGGT 
CCTCCTGGGC CGTACGGGAA CCCAGGCCCA CCTGGCCCTC CAGGAGCCAA AGGACAGAAA 
GGGGACCCAG GACTCTCACC AGGACAGGCT CACGATGGAG CAAAGGGCAA CATGGGCTTG 
CCTGGGCTCT CCGGAAATCC AGGACCCTTG GGAAGGAAGG GACACAAGGG CCATCCTGGA 
GCAGCAGGGC ACCCTGGAGA ACAGGGGCAG CCAGGACCTG AGGGCAGTCC AGGGGCCAAA 
GGTTACCCTG GCAGGCAGGG GTTCCCTGGA CCTGTAGGAG ACCCTGGCCC CAAAGGCAGC 
AGGGGTTACA TTGGTCTTCC GGGGCTCTTT GGCCTGCCAG GGTCGGATGG AGAGCGGGGT 
CTCCCTGGCG TTCCTGGCAA GAGGGGCGAG ATGGGTAGGC CGGGCTTCCC TGGAGACTTT 
GGGGAAAGAG GCCCCCCTGG ACTTGATGGC AACCCTGGAG AAATTGGCCT GCCGGGGCCA 
CCAGGAGTGC TCGGGCTCAT TGGTGACACA GGAGCACTGG GCCCAGTTGG CTACCCAGGA 
CCGAAAGGCA TGAAGGGACT GATGGGCGGC GTGGGGGAGC CCGGACTGAA AGGTGATAAG 
GGTGAACAAG GGGTGCCAGG TGTGTCGGGA GATCCTGGCT TCCAAGGAGA CAAGGGGAGT 
CATGGCTTGC CAGGGCTCCC GGGTGGCCGG GGGAAGCCAG GGCCTCTGGG AAAAGCTGGA 
GACAAAGGAT CACTTGGGTT TCCTGGACCT CCTGGACCTG AGGGTTTCCC AGGAGACATT 
GGCCCTCCAG GGGACAATGG CCCTGAAGGC ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG 
CCTGGACCTC CTGGGCAGCT GGGGCCTGAG GGAGATGAAG GACCCATGGG GCCACCAGGA 
GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT 
GGCTCGAAGG GGGAACCAGG GGATCCTGGA CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT 
ATGGGATTCA TTGGTCTTGT CGGAGAGCCT GGAATTGTTG GGGAGAAGGG AGACCGTGGG 
GTGATGGGAC CCCCAGGTGC ACCTGGACCC AAGGGGTCGA TGGGCCATCC TGGAACACCC 
GGTGGTATTG GGAACCCTGG AGAGCCTGGA CCCTGGGGCC CTCCAGGATC TCGAGGCCTG 
CCAGGCATGA GGGGAGCCAA GGGGCACCGG GGCCCTCGAG GACCTGATGG ACCAGCTGGG 
GAGCAGGGAT CCAAGGGCCT GAAGGGTCGT GTAGGACCTC GGGGCAGACC TGGCCAGCCA 
GGACAGCAGG GTGCAGCTGG TGAGCGAGGC CACTCAGGTG CAAAAGGCTT CCTTGGCATC 
CCTGGTCCCT CAGGCCCTCC AGGTGCCAAG GGCCTCCCAG GAGAACCGGG CTCTCAGGGA 
CCTCAGGGGC CAGTTGGTCC TCCAGGAGAA ATGGGGCCCA AGGGACCGCC GGGTGCAGTG 
GGAGAGCCTG GCCTTCCTGG GGACTCCGGA ATGAAGGGTG ACCTCGGACC TCTGGGCCCC 
CCTGGGGAGC AAGGTCTTAT TGGGCAGCGG GGAGAGCCAG GCCTTGAGGG AGACCATGGC 
CCTGTGGGGC CAGATGGGCT GAAGGGCGAC AGGGGCGACC CAGGGCCCGA TGGCGAGCAT 
GGTGAAAAGG GCCAGGAAGG GCTGAAAGGA GAGGATGGGT CCCCTGGGCC CCCGGGCATC 
ACTGGCGTCC CGGGTCGTGA GGGGAAGCCG GGCAAGCAGG GTGAGAAAGG CCAGAGGGGA 
GCTAAGGGTG CCAAGGGCCA CCAAGGATAT CTGGGAGAGA TGGGCATCCC CGGAGAACCT 
GGGCCCCCTG GGACCCCAGG TCCTAAAGGG TCCCGGGGCA CCCTAGGACC AACGGGTGCT 
CCAGGACGGA TGGGAGCCCA AGGAGAACCA GGATTGGCCG GTTATAATGG ACACAAAGGC 
ATCACAGGAC CCCTCGGGCC TCCCGGGCCC AAAGGCGAGA AGGGGGACCA GGGCGAGGAT 
GGCAAGACCG AGGGGCCCCC TGGGCCACCA GGAGATCGGG GTCCTGTGGG TGATCGAGGA 
GACCGTGGGG AGCCAGGCGA CCCTGGATAC CCTGGTCAAG AGGGAGTTCA AGGCCTCCGT 
GGAGAACCGG GCCAGCAGGG ACAGCCTGGG CATCCAGGAC CCCGGGGGCG CCCAGGACCC 
AAAGGATCAA AAGGCGAAGA GGGCCCAAAG GGAAAGCCAG GCAAGGCTGG GCCATCAGGC 
CGGAGGGGGA CCCAGGTGAG TTGCCATAGC TTCTCTGTCA CCTTCTATGT TCTCCTGAGA 
AGGTTTAACC AGCTTCTTCT GTACCTCCAG GGCCTTCAAG GACTGCCAGG CCCCCGAGGC 
GTGGTGGGGA GACAGGGCCC TGAGGGCACT GCTGGATCTG ATGGGATTCC TGGCAGAGAT 
GGTCGGCCAG GATATCAGGG AGACCAGGGA AATGATGGGG ACCCTGGCCC TGTGGGCCCT 
GCTGGGAGAA GAGGAAATCC AGGTGTGGCT GGCTTGCCTG GAGCACAGGG GCCTCCGGGA 
TTCAAGGGTG AAAGTGGGTT ACCTGGGCAA CTGGGTCCCC CTGGAAAACG AGGGACAGAA 
GGTGGAACAG GGCTTCCTGG GAACCAGGGG GAGCCAGGAT CCAAAGGCCA GCCGGGTGAC 
TCTGGCGAGA TGGGCTTCCC AGGAGTGGCT GGCCTCTTTG GACCCAAGGG CCCCCCTGGA 
GACATTGGCT TCAAAGGCAT CCAAGGTCCT CGGGGGCCTC CTGGCTTGAT GGGAAAGGAA 
GGTATCATTG GCCCCCCCGG AATGCTGGGA CCTTCTGGAC TCCCGGGTCC CAAAGGTGAC 
AGAGGCAGCC GAGGAGACTT GGGACTGCAA GGCCCAAGGG GTCCTCCTGG TCCAAGGGGC 
CGGCCAGGTC CCCCGGGCCC TCCTTGGCAT CCCATCCAGT TTCAGCAAGA TGACCTTGGA 
GCAGCTTTCC AGACATGGAT GGATGCTCAA GGAGCCGTCA GATCGGAGCA GGGGTACAGC 
TATCCGGACC AGCTGGCGCT AGACCAGGGA GGGGAGATCT TCAAAACCTT ACACTACCTC 
AGCAACCTCA TCCAGAGCAT TAAGACACCC TTGGGCACCA AGGAGAACCC AGCCCGGGTC 
TGCCGGGACC TCATGGACTG TGAGCAGAGG ATGGCGGATG GTACCTACTG GGTGGACCCC 
AACCTCGGCT GCTCCTCTGA CACCATTGAA GTCTCCTGCA ACTTTACACA GGGTGGGCAG 
ACGTGCCTGA AGCCCATCAC GGCCTCCAAG GCCGAGTTTG CTGTGAGTCG GGTCCAGATG 
AATTTCTTGC ACCTGCTGAG CTCTGAGGGG ACACAGCATA TCACAATCCA CTGTCTGAAC 
ATGACGGTGT GGCAGGAGGG ACCGGGACGC TCCTCTGCCA GACAGGCTGT GCGCTTCCGT 
GCCTGGAACG GACAGGTCTT CGAAGCTGGG GGTCAGTTCA GGCCAGAGGT GTCTATGGAT 
GGCTGCAAGG TCCATGATGG CCGCTGGCAT CAGACACTGT TCACCTTCCG GACCCAGGAC 
CCCCAGCAGC TGCCCATCGT CAGTGTGGAC AATCTCCCGC CTGTCTCATC AGGGAAGCAG 
TACCGCCTGG AAGTTGGACC TGCGTGCTTC CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col27a1 ( XM_017320283.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175772.1 Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu81037
Clone ID Related Accession (Same CDS sequence)	XM_017320283.1
Accession Version	XM_017320283.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5616bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	collagen alpha-1(XXVII) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGTTAG GCACCCGCCT AAGAACCGAC CGGCCAGCGT CACACTTGGG GACCCAGGGG 
TTTCTCTTCG CCTGGATCTT GGTCTCATTT ACCTGTCACC TGGCCTCCAC CCAAGGAGCT 
CCTGAAGATG TGGATGTCCT CCAGCGGCTG GGCCTCAGCT GGACGAAGGC CGGGGGTGGC 
CGGAGTCCTA CACCCCCTGG TGTCATTCCT TTCCCATCTG GCTTCATCTT CACACAGCGG 
GCCAAGCTGC AGGCCCCCAC AGCCAATGTT CTTCCCACCA CCCTGGGGCG GGAGCTGGCA 
TTGGTGTTGA GCCTCTGTTC ACATCGTGTG AACCATGCCT TCCTCTTTGC CATCCGCAGC 
AGGAAACACA AGCTGCAACT GGGTCTGCAG TTCCTCCCCG GCAGGACGAT CATCCACCTG 
GGGCCTCGGC AATCAGTGGC CTTCGACTTG GATGTGCATG ATGGGCGCTG GCACCACCTG 
GCCCTAGAGT TGCGTGGCCG CACAGTCACA ATGGTGACAG CCTGTGGGCA GCACAGGGTA 
CCTGTCCCAC TGCCTTCCCG TAGGGACTCC ATGCTTGACC CCCAGGGCTC CTTTCTCTTG 
GGGAAGGTGA ACCCCCGTGC TGTCCAGTTT GAAGGTGCAC TCTGTCAGTT CAGCATCCAC 
CCTGTGGCGC AGGTCGCTCA CAATTACTGT GCCCACCTGA GAGAGCGGTG CCGACAGGTG 
GACACATATA GTCCCCAGGT GGGAACCCTC TTTCCTTGGG ACTCTGGCCC AGCCTTTGCT 
TTGCATCCTG AACCAGCCTT GCTTGGCTTG GGGAATCTGA CCAGAACTCC AGCAACCCTG 
GGGGCCAGGC CTGTAAGCAG GGCACTTGCA GTGACTCTGG CTCCTGCCAT GCCCACCAAG 
CCCCTGAGGA CTGTCCACCC AGATGTCTCT GAACACAGCT CTTCCCAGAC CCCACTGTCA 
CCTGCCAAAC AGTCGGCCAG GAAAACACCT TCCCCCTCCT CGTCAGCTTC CCTGGCTAAT 
TCCACCAGGG TTTATCGTCC AGCTGCTGCC CAGCCAAGAC AGATCACAAC TACTAGCCCC 
ACCAAACGTT CCCCCACCAA GCCTTCTGTT TCTCCCCTTT CAGTTACCCC CATGAAAAGT 
CCCCATGCAA CCCAGAAAAC AGGTGTCCCT TCATTCACAA AGCCTGTTCC ACCCACTCAG 
AAGCCAGCGC CCTTCACCTC CTACCTAGCA CCTTCCAAAG CCTCTAGTCC CACTGTGAGG 
CCTGTTCAGA AGACTTTCAT GACACCCCGA CCTCCAGTCC CCAGCCCCCA GCCCCTGCGC 
CCTACTACTG GCTTATCTAA GAAGTTCACT AACCCCACTG TTGCAAAATC TAAGTCCAAG 
ACGACCAGTT GGGCCAGCAA GCCGGTCTTG GCCCGCTCCA GTGTCCCTAA AACGCTTCAG 
CAAACTGTTT TGTCCCAGTC TCCTGTTTCC TATCTGGGCT CTCAGACACT GGCTCCAGCC 
CTCCCTCCTT TGGGTGTTGG AAATCCTAGA ACGATGCCTC CCACACGTGA CTCTGCTTTA 
ACTCCTGCTG GAAGCAAGAA ATTCACAGGA CGGGAAACCT CCAAGAAAAC CAGACAGAAG 
AGCAGCCCCC GGAAGCCCGA ACCTCTCAGC CCTGGGAAGT CAGCCAGGGA TGCCTCACCA 
AGAGACCTGA CAACCAAGCC TAGCCGGCCG TCCACCCCAG CCCTGGTCCT TGCCCCAGCT 
TACCTCCTGT CCTCCAGCCC CCAGCCCACC AGCAGCAGCT TCCCTTTCTT CCACCTGCTG 
GGCCCTACAC CTTTCCCGAT GCTGATGGGG CCTCCAGGCT CCAAGGGAGA CTGTGGCTTG 
CCGGGTCCCC CTGGGCTTCC AGGATTACCT GGATCTCCGG GCGCACGAGG TCCTCGGGGT 
CCTCCTGGGC CGTACGGGAA CCCAGGCCCA CCTGGCCCTC CAGGAGCCAA AGGACAGAAA 
GGGGACCCAG GACTCTCACC AGGACAGGCT CACGATGGAG CAAAGGGCAA CATGGGCTTG 
CCTGGGCTCT CCGGAAATCC AGGACCCTTG GGAAGGAAGG GACACAAGGG CCATCCTGGA 
GCAGCAGGGC ACCCTGGAGA ACAGGGGCAG CCAGGACCTG AGGGCAGTCC AGGGGCCAAA 
GGTTACCCTG GCAGGCAGGG GTTCCCTGGA CCTGTAGGAG ACCCTGGCCC CAAAGGCAGC 
AGGGGTTACA TTGGTCTTCC GGGGCTCTTT GGCCTGCCAG GGTCGGATGG AGAGCGGGGT 
CTCCCTGGCG TTCCTGGCAA GAGGGGCGAG ATGGGTAGGC CGGGCTTCCC TGGAGACTTT 
GGGGAAAGAG GCCCCCCTGG ACTTGATGGC AACCCTGGAG AAATTGGCCT GCCGGGGCCA 
CCAGGAGTGC TCGGGCTCAT TGGTGACACA GGAGCACTGG GCCCAGTTGG CTACCCAGGA 
CCGAAAGGCA TGAAGGGACT GATGGGCGGC GTGGGGGAGC CCGGACTGAA AGGTGATAAG 
GGTGAACAAG GGGTGCCAGG TGTGTCGGGA GATCCTGGCT TCCAAGGAGA CAAGGGGAGT 
CATGGCTTGC CAGGGCTCCC GGGTGGCCGG GGGAAGCCAG GGCCTCTGGG AAAAGCTGGA 
GACAAAGGAT CACTTGGGTT TCCTGGACCT CCTGGACCTG AGGGTTTCCC AGGAGACATT 
GGCCCTCCAG GGGACAATGG CCCTGAAGGC ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG 
CCTGGACCTC CTGGGCAGCT GGGGCCTGAG GGAGATGAAG GACCCATGGG GCCACCAGGA 
GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT 
GGCTCGAAGG GGGAACCAGG GGATCCTGGA CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT 
ATGGGATTCA TTGGTCTTGT CGGAGAGCCT GGAATTGTTG GGGAGAAGGG AGACCGTGGG 
GTGATGGGAC CCCCAGGTGC ACCTGGACCC AAGGGGTCGA TGGGCCATCC TGGAACACCC 
GGTGGTATTG GGAACCCTGG AGAGCCTGGA CCCTGGGGCC CTCCAGGATC TCGAGGCCTG 
CCAGGCATGA GGGGAGCCAA GGGGCACCGG GGCCCTCGAG GACCTGATGG ACCAGCTGGG 
GAGCAGGGAT CCAAGGGCCT GAAGGGTCGT GTAGGACCTC GGGGCAGACC TGGCCAGCCA 
GGACAGCAGG GTGCAGCTGG TGAGCGAGGC CACTCAGGTG CAAAAGGCTT CCTTGGCATC 
CCTGGTCCCT CAGGCCCTCC AGGTGCCAAG GGCCTCCCAG GAGAACCGGG CTCTCAGGGA 
CCTCAGGGGC CAGTTGGTCC TCCAGGAGAA ATGGGGCCCA AGGGACCGCC GGGTGCAGTG 
GGAGAGCCTG GCCTTCCTGG GGACTCCGGA ATGAAGGGTG ACCTCGGACC TCTGGGCCCC 
CCTGGGGAGC AAGGTCTTAT TGGGCAGCGG GGAGAGCCAG GCCTTGAGGG AGACCATGGC 
CCTGTGGGGC CAGATGGGCT GAAGGGCGAC AGGGGCGACC CAGGGCCCGA TGGCGAGCAT 
GGTGAAAAGG GCCAGGAAGG GCTGAAAGGA GAGGATGGGT CCCCTGGGCC CCCGGGCATC 
ACTGGCGTCC CGGGTCGTGA GGGGAAGCCG GGCAAGCAGG GTGAGAAAGG CCAGAGGGGA 
GCTAAGGGTG CCAAGGGCCA CCAAGGATAT CTGGGAGAGA TGGGCATCCC CGGAGAACCT 
GGGCCCCCTG GGACCCCAGG TCCTAAAGGG TCCCGGGGCA CCCTAGGACC AACGGGTGCT 
CCAGGACGGA TGGGAGCCCA AGGAGAACCA GGATTGGCCG GTTATAATGG ACACAAAGGC 
ATCACAGGAC CCCTCGGGCC TCCCGGGCCC AAAGGCGAGA AGGGGGACCA GGGCGAGGAT 
GGCAAGACCG AGGGGCCCCC TGGGCCACCA GGAGATCGGG GTCCTGTGGG TGATCGAGGA 
GACCGTGGGG AGCCAGGCGA CCCTGGATAC CCTGGTCAAG AGGGAGTTCA AGGCCTCCGT 
GGAGAACCGG GCCAGCAGGG ACAGCCTGGG CATCCAGGAC CCCGGGGGCG CCCAGGACCC 
AAAGGATCAA AAGGCGAAGA GGGCCCAAAG GGAAAGCCAG GCAAGGCTGG GCCATCAGGC 
CGGAGGGGGA CCCAGGTGAG TTGCCATAGC TTCTCTGTCA CCTTCTATGT TCTCCTGAGA 
AGGTTTAACC AGCTTCTTCT GTACCTCCAG GGCCTTCAAG GACTGCCAGG CCCCCGAGGC 
GTGGTGGGGA GACAGGGCCC TGAGGGCACT GCTGGATCTG ATGGGATTCC TGGCAGAGAT 
GGTCGGCCAG GATATCAGGG AGACCAGGGA AATGATGGGG ACCCTGGCCC TGTGGGCCCT 
GCTGGGAGAA GAGGAAATCC AGGTGTGGCT GGCTTGCCTG GAGCACAGGG GCCTCCGGGA 
TTCAAGGGTG AAAGTGGGTT ACCTGGGCAA CTGGGTCCCC CTGGAAAACG AGGGACAGAA 
GGTGGAACAG GGCTTCCTGG GAACCAGGGG GAGCCAGGAT CCAAAGGCCA GCCGGGTGAC 
TCTGGCGAGA TGGGCTTCCC AGGAGTGGCT GGCCTCTTTG GACCCAAGGG CCCCCCTGGA 
GACATTGGCT TCAAAGGCAT CCAAGGTCCT CGGGGGCCTC CTGGCTTGAT GGGAAAGGAA 
GGTATCATTG GCCCCCCCGG AATGCTGGGA CCTTCTGGAC TCCCGGGTCC CAAAGGTGAC 
AGAGGCAGCC GAGGAGACTT GGGACTGCAA GGCCCAAGGG GTCCTCCTGG TCCAAGGGGC 
CGGCCAGGTC CCCCGGGCCC TCCTTGGCAT CCCATCCAGT TTCAGCAAGA TGACCTTGGA 
GCAGCTTTCC AGACATGGAT GGATGCTCAA GGAGCCGTCA GATCGGAGCA GGGGTACAGC 
TATCCGGACC AGCTGGCGCT AGACCAGGGA GGGGAGATCT TCAAAACCTT ACACTACCTC 
AGCAACCTCA TCCAGAGCAT TAAGACACCC TTGGGCACCA AGGAGAACCC AGCCCGGGTC 
TGCCGGGACC TCATGGACTG TGAGCAGAGG ATGGCGGATG GTACCTACTG GGTGGACCCC 
AACCTCGGCT GCTCCTCTGA CACCATTGAA GTCTCCTGCA ACTTTACACA GGGTGGGCAG 
ACGTGCCTGA AGCCCATCAC GGCCTCCAAG GCCGAGTTTG CTGTGAGTCG GGTCCAGATG 
AATTTCTTGC ACCTGCTGAG CTCTGAGGGG ACACAGCATA TCACAATCCA CTGTCTGAAC 
ATGACGGTGT GGCAGGAGGG ACCGGGACGC TCCTCTGCCA GACAGGCTGT GCGCTTCCGT 
GCCTGGAACG GACAGGTCTT CGAAGCTGGG GGTCAGTTCA GGCCAGAGGT GTCTATGGAT 
GGCTGCAAGG TCCATGATGG CCGCTGGCAT CAGACACTGT TCACCTTCCG GACCCAGGAC 
CCCCAGCAGC TGCCCATCGT CAGTGTGGAC AATCTCCCGC CTGTCTCATC AGGGAAGCAG 
TACCGCCTGG AAGTTGGACC TGCGTGCTTC CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175772.1
CDS	341..5956
Translation	MPLGTRLRTDRPASHLGTQGFLFAWILVSFTCHLASTQGAPEDVDVLQRLGLSWTKAGGGRSPTPPGVIPFPSGFIFTQRAKLQAPTANVLPTTLGRELALVLSLCSHRVNHAFLFAIRSRKHKLQLGLQFLPGRTIIHLGPRQSVAFDLDVHDGRWHHLALELRGRTVTMVTACGQHRVPVPLPSRRDSMLDPQGSFLLGKVNPRAVQFEGALCQFSIHPVAQVAHNYCAHLRERCRQVDTYSPQVGTLFPWDSGPAFALHPEPALLGLGNLTRTPATLGARPVSRALAVTLAPAMPTKPLRTVHPDVSEHSSSQTPLSPAKQSARKTPSPSSSASLANSTRVYRPAAAQPRQITTTSPTKRSPTKPSVSPLSVTPMKSPHATQKTGVPSFTKPVPPTQKPAPFTSYLAPSKASSPTVRPVQKTFMTPRPPVPSPQPLRPTTGLSKKFTNPTVAKSKSKTTSWASKPVLARSSVPKTLQQTVLSQSPVSYLGSQTLAPALPPLGVGNPRTMPPTRDSALTPAGSKKFTGRETSKKTRQKSSPRKPEPLSPGKSARDASPRDLTTKPSRPSTPALVLAPAYLLSSSPQPTSSSFPFFHLLGPTPFPMLMGPPGSKGDCGLPGPPGLPGLPGSPGARGPRGPPGPYGNPGPPGPPGAKGQKGDPGLSPGQAHDGAKGNMGLPGLSGNPGPLGRKGHKGHPGAAGHPGEQGQPGPEGSPGAKGYPGRQGFPGPVGDPGPKGSRGYIGLPGLFGLPGSDGERGLPGVPGKRGEMGRPGFPGDFGERGPPGLDGNPGEIGLPGPPGVLGLIGDTGALGPVGYPGPKGMKGLMGGVGEPGLKGDKGEQGVPGVSGDPGFQGDKGSHGLPGLPGGRGKPGPLGKAGDKGSLGFPGPPGPEGFPGDIGPPGDNGPEGMKGKPGARGLPGPPGQLGPEGDEGPMGPPGVPGLEGQPGRKGFPGRPGLDGSKGEPGDPGRPGPVGEQGLMGFIGLVGEPGIVGEKGDRGVMGPPGAPGPKGSMGHPGTPGGIGNPGEPGPWGPPGSRGLPGMRGAKGHRGPRGPDGPAGEQGSKGLKGRVGPRGRPGQPGQQGAAGERGHSGAKGFLGIPGPSGPPGAKGLPGEPGSQGPQGPVGPPGEMGPKGPPGAVGEPGLPGDSGMKGDLGPLGPPGEQGLIGQRGEPGLEGDHGPVGPDGLKGDRGDPGPDGEHGEKGQEGLKGEDGSPGPPGITGVPGREGKPGKQGEKGQRGAKGAKGHQGYLGEMGIPGEPGPPGTPGPKGSRGTLGPTGAPGRMGAQGEPGLAGYNGHKGITGPLGPPGPKGEKGDQGEDGKTEGPPGPPGDRGPVGDRGDRGEPGDPGYPGQEGVQGLRGEPGQQGQPGHPGPRGRPGPKGSKGEEGPKGKPGKAGPSGRRGTQVSCHSFSVTFYVLLRRFNQLLLYLQGLQGLPGPRGVVGRQGPEGTAGSDGIPGRDGRPGYQGDQGNDGDPGPVGPAGRRGNPGVAGLPGAQGPPGFKGESGLPGQLGPPGKRGTEGGTGLPGNQGEPGSKGQPGDSGEMGFPGVAGLFGPKGPPGDIGFKGIQGPRGPPGLMGKEGIIGPPGMLGPSGLPGPKGDRGSRGDLGLQGPRGPPGPRGRPGPPGPPWHPIQFQQDDLGAAFQTWMDAQGAVRSEQGYSYPDQLALDQGGEIFKTLHYLSNLIQSIKTPLGTKENPARVCRDLMDCEQRMADGTYWVDPNLGCSSDTIEVSCNFTQGGQTCLKPITASKAEFAVSRVQMNFLHLLSSEGTQHITIHCLNMTVWQEGPGRSSARQAVRFRAWNGQVFEAGGQFRPEVSMDGCKVHDGRWHQTLFTFRTQDPQQLPIVSVDNLPPVSSGKQYRLEVGPACFL

Target ORF information:

RefSeq Version	XM_017320283.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320283.1

ORF Insert Sequence:

ATGCCGTTAG GCACCCGCCT AAGAACCGAC CGGCCAGCGT CACACTTGGG GACCCAGGGG 
TTTCTCTTCG CCTGGATCTT GGTCTCATTT ACCTGTCACC TGGCCTCCAC CCAAGGAGCT 
CCTGAAGATG TGGATGTCCT CCAGCGGCTG GGCCTCAGCT GGACGAAGGC CGGGGGTGGC 
CGGAGTCCTA CACCCCCTGG TGTCATTCCT TTCCCATCTG GCTTCATCTT CACACAGCGG 
GCCAAGCTGC AGGCCCCCAC AGCCAATGTT CTTCCCACCA CCCTGGGGCG GGAGCTGGCA 
TTGGTGTTGA GCCTCTGTTC ACATCGTGTG AACCATGCCT TCCTCTTTGC CATCCGCAGC 
AGGAAACACA AGCTGCAACT GGGTCTGCAG TTCCTCCCCG GCAGGACGAT CATCCACCTG 
GGGCCTCGGC AATCAGTGGC CTTCGACTTG GATGTGCATG ATGGGCGCTG GCACCACCTG 
GCCCTAGAGT TGCGTGGCCG CACAGTCACA ATGGTGACAG CCTGTGGGCA GCACAGGGTA 
CCTGTCCCAC TGCCTTCCCG TAGGGACTCC ATGCTTGACC CCCAGGGCTC CTTTCTCTTG 
GGGAAGGTGA ACCCCCGTGC TGTCCAGTTT GAAGGTGCAC TCTGTCAGTT CAGCATCCAC 
CCTGTGGCGC AGGTCGCTCA CAATTACTGT GCCCACCTGA GAGAGCGGTG CCGACAGGTG 
GACACATATA GTCCCCAGGT GGGAACCCTC TTTCCTTGGG ACTCTGGCCC AGCCTTTGCT 
TTGCATCCTG AACCAGCCTT GCTTGGCTTG GGGAATCTGA CCAGAACTCC AGCAACCCTG 
GGGGCCAGGC CTGTAAGCAG GGCACTTGCA GTGACTCTGG CTCCTGCCAT GCCCACCAAG 
CCCCTGAGGA CTGTCCACCC AGATGTCTCT GAACACAGCT CTTCCCAGAC CCCACTGTCA 
CCTGCCAAAC AGTCGGCCAG GAAAACACCT TCCCCCTCCT CGTCAGCTTC CCTGGCTAAT 
TCCACCAGGG TTTATCGTCC AGCTGCTGCC CAGCCAAGAC AGATCACAAC TACTAGCCCC 
ACCAAACGTT CCCCCACCAA GCCTTCTGTT TCTCCCCTTT CAGTTACCCC CATGAAAAGT 
CCCCATGCAA CCCAGAAAAC AGGTGTCCCT TCATTCACAA AGCCTGTTCC ACCCACTCAG 
AAGCCAGCGC CCTTCACCTC CTACCTAGCA CCTTCCAAAG CCTCTAGTCC CACTGTGAGG 
CCTGTTCAGA AGACTTTCAT GACACCCCGA CCTCCAGTCC CCAGCCCCCA GCCCCTGCGC 
CCTACTACTG GCTTATCTAA GAAGTTCACT AACCCCACTG TTGCAAAATC TAAGTCCAAG 
ACGACCAGTT GGGCCAGCAA GCCGGTCTTG GCCCGCTCCA GTGTCCCTAA AACGCTTCAG 
CAAACTGTTT TGTCCCAGTC TCCTGTTTCC TATCTGGGCT CTCAGACACT GGCTCCAGCC 
CTCCCTCCTT TGGGTGTTGG AAATCCTAGA ACGATGCCTC CCACACGTGA CTCTGCTTTA 
ACTCCTGCTG GAAGCAAGAA ATTCACAGGA CGGGAAACCT CCAAGAAAAC CAGACAGAAG 
AGCAGCCCCC GGAAGCCCGA ACCTCTCAGC CCTGGGAAGT CAGCCAGGGA TGCCTCACCA 
AGAGACCTGA CAACCAAGCC TAGCCGGCCG TCCACCCCAG CCCTGGTCCT TGCCCCAGCT 
TACCTCCTGT CCTCCAGCCC CCAGCCCACC AGCAGCAGCT TCCCTTTCTT CCACCTGCTG 
GGCCCTACAC CTTTCCCGAT GCTGATGGGG CCTCCAGGCT CCAAGGGAGA CTGTGGCTTG 
CCGGGTCCCC CTGGGCTTCC AGGATTACCT GGATCTCCGG GCGCACGAGG TCCTCGGGGT 
CCTCCTGGGC CGTACGGGAA CCCAGGCCCA CCTGGCCCTC CAGGAGCCAA AGGACAGAAA 
GGGGACCCAG GACTCTCACC AGGACAGGCT CACGATGGAG CAAAGGGCAA CATGGGCTTG 
CCTGGGCTCT CCGGAAATCC AGGACCCTTG GGAAGGAAGG GACACAAGGG CCATCCTGGA 
GCAGCAGGGC ACCCTGGAGA ACAGGGGCAG CCAGGACCTG AGGGCAGTCC AGGGGCCAAA 
GGTTACCCTG GCAGGCAGGG GTTCCCTGGA CCTGTAGGAG ACCCTGGCCC CAAAGGCAGC 
AGGGGTTACA TTGGTCTTCC GGGGCTCTTT GGCCTGCCAG GGTCGGATGG AGAGCGGGGT 
CTCCCTGGCG TTCCTGGCAA GAGGGGCGAG ATGGGTAGGC CGGGCTTCCC TGGAGACTTT 
GGGGAAAGAG GCCCCCCTGG ACTTGATGGC AACCCTGGAG AAATTGGCCT GCCGGGGCCA 
CCAGGAGTGC TCGGGCTCAT TGGTGACACA GGAGCACTGG GCCCAGTTGG CTACCCAGGA 
CCGAAAGGCA TGAAGGGACT GATGGGCGGC GTGGGGGAGC CCGGACTGAA AGGTGATAAG 
GGTGAACAAG GGGTGCCAGG TGTGTCGGGA GATCCTGGCT TCCAAGGAGA CAAGGGGAGT 
CATGGCTTGC CAGGGCTCCC GGGTGGCCGG GGGAAGCCAG GGCCTCTGGG AAAAGCTGGA 
GACAAAGGAT CACTTGGGTT TCCTGGACCT CCTGGACCTG AGGGTTTCCC AGGAGACATT 
GGCCCTCCAG GGGACAATGG CCCTGAAGGC ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG 
CCTGGACCTC CTGGGCAGCT GGGGCCTGAG GGAGATGAAG GACCCATGGG GCCACCAGGA 
GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT 
GGCTCGAAGG GGGAACCAGG GGATCCTGGA CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT 
ATGGGATTCA TTGGTCTTGT CGGAGAGCCT GGAATTGTTG GGGAGAAGGG AGACCGTGGG 
GTGATGGGAC CCCCAGGTGC ACCTGGACCC AAGGGGTCGA TGGGCCATCC TGGAACACCC 
GGTGGTATTG GGAACCCTGG AGAGCCTGGA CCCTGGGGCC CTCCAGGATC TCGAGGCCTG 
CCAGGCATGA GGGGAGCCAA GGGGCACCGG GGCCCTCGAG GACCTGATGG ACCAGCTGGG 
GAGCAGGGAT CCAAGGGCCT GAAGGGTCGT GTAGGACCTC GGGGCAGACC TGGCCAGCCA 
GGACAGCAGG GTGCAGCTGG TGAGCGAGGC CACTCAGGTG CAAAAGGCTT CCTTGGCATC 
CCTGGTCCCT CAGGCCCTCC AGGTGCCAAG GGCCTCCCAG GAGAACCGGG CTCTCAGGGA 
CCTCAGGGGC CAGTTGGTCC TCCAGGAGAA ATGGGGCCCA AGGGACCGCC GGGTGCAGTG 
GGAGAGCCTG GCCTTCCTGG GGACTCCGGA ATGAAGGGTG ACCTCGGACC TCTGGGCCCC 
CCTGGGGAGC AAGGTCTTAT TGGGCAGCGG GGAGAGCCAG GCCTTGAGGG AGACCATGGC 
CCTGTGGGGC CAGATGGGCT GAAGGGCGAC AGGGGCGACC CAGGGCCCGA TGGCGAGCAT 
GGTGAAAAGG GCCAGGAAGG GCTGAAAGGA GAGGATGGGT CCCCTGGGCC CCCGGGCATC 
ACTGGCGTCC CGGGTCGTGA GGGGAAGCCG GGCAAGCAGG GTGAGAAAGG CCAGAGGGGA 
GCTAAGGGTG CCAAGGGCCA CCAAGGATAT CTGGGAGAGA TGGGCATCCC CGGAGAACCT 
GGGCCCCCTG GGACCCCAGG TCCTAAAGGG TCCCGGGGCA CCCTAGGACC AACGGGTGCT 
CCAGGACGGA TGGGAGCCCA AGGAGAACCA GGATTGGCCG GTTATAATGG ACACAAAGGC 
ATCACAGGAC CCCTCGGGCC TCCCGGGCCC AAAGGCGAGA AGGGGGACCA GGGCGAGGAT 
GGCAAGACCG AGGGGCCCCC TGGGCCACCA GGAGATCGGG GTCCTGTGGG TGATCGAGGA 
GACCGTGGGG AGCCAGGCGA CCCTGGATAC CCTGGTCAAG AGGGAGTTCA AGGCCTCCGT 
GGAGAACCGG GCCAGCAGGG ACAGCCTGGG CATCCAGGAC CCCGGGGGCG CCCAGGACCC 
AAAGGATCAA AAGGCGAAGA GGGCCCAAAG GGAAAGCCAG GCAAGGCTGG GCCATCAGGC 
CGGAGGGGGA CCCAGGTGAG TTGCCATAGC TTCTCTGTCA CCTTCTATGT TCTCCTGAGA 
AGGTTTAACC AGCTTCTTCT GTACCTCCAG GGCCTTCAAG GACTGCCAGG CCCCCGAGGC 
GTGGTGGGGA GACAGGGCCC TGAGGGCACT GCTGGATCTG ATGGGATTCC TGGCAGAGAT 
GGTCGGCCAG GATATCAGGG AGACCAGGGA AATGATGGGG ACCCTGGCCC TGTGGGCCCT 
GCTGGGAGAA GAGGAAATCC AGGTGTGGCT GGCTTGCCTG GAGCACAGGG GCCTCCGGGA 
TTCAAGGGTG AAAGTGGGTT ACCTGGGCAA CTGGGTCCCC CTGGAAAACG AGGGACAGAA 
GGTGGAACAG GGCTTCCTGG GAACCAGGGG GAGCCAGGAT CCAAAGGCCA GCCGGGTGAC 
TCTGGCGAGA TGGGCTTCCC AGGAGTGGCT GGCCTCTTTG GACCCAAGGG CCCCCCTGGA 
GACATTGGCT TCAAAGGCAT CCAAGGTCCT CGGGGGCCTC CTGGCTTGAT GGGAAAGGAA 
GGTATCATTG GCCCCCCCGG AATGCTGGGA CCTTCTGGAC TCCCGGGTCC CAAAGGTGAC 
AGAGGCAGCC GAGGAGACTT GGGACTGCAA GGCCCAAGGG GTCCTCCTGG TCCAAGGGGC 
CGGCCAGGTC CCCCGGGCCC TCCTTGGCAT CCCATCCAGT TTCAGCAAGA TGACCTTGGA 
GCAGCTTTCC AGACATGGAT GGATGCTCAA GGAGCCGTCA GATCGGAGCA GGGGTACAGC 
TATCCGGACC AGCTGGCGCT AGACCAGGGA GGGGAGATCT TCAAAACCTT ACACTACCTC 
AGCAACCTCA TCCAGAGCAT TAAGACACCC TTGGGCACCA AGGAGAACCC AGCCCGGGTC 
TGCCGGGACC TCATGGACTG TGAGCAGAGG ATGGCGGATG GTACCTACTG GGTGGACCCC 
AACCTCGGCT GCTCCTCTGA CACCATTGAA GTCTCCTGCA ACTTTACACA GGGTGGGCAG 
ACGTGCCTGA AGCCCATCAC GGCCTCCAAG GCCGAGTTTG CTGTGAGTCG GGTCCAGATG 
AATTTCTTGC ACCTGCTGAG CTCTGAGGGG ACACAGCATA TCACAATCCA CTGTCTGAAC 
ATGACGGTGT GGCAGGAGGG ACCGGGACGC TCCTCTGCCA GACAGGCTGT GCGCTTCCGT 
GCCTGGAACG GACAGGTCTT CGAAGCTGGG GGTCAGTTCA GGCCAGAGGT GTCTATGGAT 
GGCTGCAAGG TCCATGATGG CCGCTGGCAT CAGACACTGT TCACCTTCCG GACCCAGGAC 
CCCCAGCAGC TGCCCATCGT CAGTGTGGAC AATCTCCCGC CTGTCTCATC AGGGAAGCAG 
TACCGCCTGG AAGTTGGACC TGCGTGCTTC CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col27a1 ( XM_017320284.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175773.1 Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu81038
Clone ID Related Accession (Same CDS sequence)	XM_017320284.1
Accession Version	XM_017320284.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5613bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	collagen alpha-1(XXVII) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCACGG GATTCGCGCG GGGGGCCCGA GGCACAGCGG CGTCAGGACC CGGGGGGGGG 
TTTCTCTTCG CCTGGATCTT GGTCTCATTT ACCTGTCACC TGGCCTCCAC CCAAGGAGCT 
CCTGAAGATG TGGATGTCCT CCAGCGGCTG GGCCTCAGCT GGACGAAGGC CGGGGGTGGC 
CGGAGTCCTA CACCCCCTGG TGTCATTCCT TTCCCATCTG GCTTCATCTT CACACAGCGG 
GCCAAGCTGC AGGCCCCCAC AGCCAATGTT CTTCCCACCA CCCTGGGGCG GGAGCTGGCA 
TTGGTGTTGA GCCTCTGTTC ACATCGTGTG AACCATGCCT TCCTCTTTGC CATCCGCAGC 
AGGAAACACA AGCTGCAACT GGGTCTGCAG TTCCTCCCCG GCAGGACGAT CATCCACCTG 
GGGCCTCGGC AATCAGTGGC CTTCGACTTG GATGTGCATG ATGGGCGCTG GCACCACCTG 
GCCCTAGAGT TGCGTGGCCG CACAGTCACA ATGGTGACAG CCTGTGGGCA GCACAGGGTA 
CCTGTCCCAC TGCCTTCCCG TAGGGACTCC ATGCTTGACC CCCAGGGCTC CTTTCTCTTG 
GGGAAGGTGA ACCCCCGTGC TGTCCAGTTT GAAGGTGCAC TCTGTCAGTT CAGCATCCAC 
CCTGTGGCGC AGGTCGCTCA CAATTACTGT GCCCACCTGA GAGAGCGGTG CCGACAGGTG 
GACACATATA GTCCCCAGGT GGGAACCCTC TTTCCTTGGG ACTCTGGCCC AGCCTTTGCT 
TTGCATCCTG AACCAGCCTT GCTTGGCTTG GGGAATCTGA CCAGAACTCC AGCAACCCTG 
GGGGCCAGGC CTGTAAGCAG GGCACTTGCA GTGACTCTGG CTCCTGCCAT GCCCACCAAG 
CCCCTGAGGA CTGTCCACCC AGATGTCTCT GAACACAGCT CTTCCCAGAC CCCACTGTCA 
CCTGCCAAAC AGTCGGCCAG GAAAACACCT TCCCCCTCCT CGTCAGCTTC CCTGGCTAAT 
TCCACCAGGG TTTATCGTCC AGCTGCTGCC CAGCCAAGAC AGATCACAAC TACTAGCCCC 
ACCAAACGTT CCCCCACCAA GCCTTCTGTT TCTCCCCTTT CAGTTACCCC CATGAAAAGT 
CCCCATGCAA CCCAGAAAAC AGGTGTCCCT TCATTCACAA AGCCTGTTCC ACCCACTCAG 
AAGCCAGCGC CCTTCACCTC CTACCTAGCA CCTTCCAAAG CCTCTAGTCC CACTGTGAGG 
CCTGTTCAGA AGACTTTCAT GACACCCCGA CCTCCAGTCC CCAGCCCCCA GCCCCTGCGC 
CCTACTACTG GCTTATCTAA GAAGTTCACT AACCCCACTG TTGCAAAATC TAAGTCCAAG 
ACGACCAGTT GGGCCAGCAA GCCGGTCTTG GCCCGCTCCA GTGTCCCTAA AACGCTTCAG 
CAAACTGTTT TGTCCCAGTC TCCTGTTTCC TATCTGGGCT CTCAGACACT GGCTCCAGCC 
CTCCCTCCTT TGGGTGTTGG AAATCCTAGA ACGATGCCTC CCACACGTGA CTCTGCTTTA 
ACTCCTGCTG GAAGCAAGAA ATTCACAGGA CGGGAAACCT CCAAGAAAAC CAGACAGAAG 
AGCAGCCCCC GGAAGCCCGA ACCTCTCAGC CCTGGGAAGT CAGCCAGGGA TGCCTCACCA 
AGAGACCTGA CAACCAAGCC TAGCCGGCCG TCCACCCCAG CCCTGGTCCT TGCCCCAGCT 
TACCTCCTGT CCTCCAGCCC CCAGCCCACC AGCAGCAGCT TCCCTTTCTT CCACCTGCTG 
GGCCCTACAC CTTTCCCGAT GCTGATGGGG CCTCCAGGCT CCAAGGGAGA CTGTGGCTTG 
CCGGGTCCCC CTGGGCTTCC AGGATTACCT GGATCTCCGG GCGCACGAGG TCCTCGGGGT 
CCTCCTGGGC CGTACGGGAA CCCAGGCCCA CCTGGCCCTC CAGGAGCCAA AGGACAGAAA 
GGGGACCCAG GACTCTCACC AGGACAGGCT CACGATGGAG CAAAGGGCAA CATGGGCTTG 
CCTGGGCTCT CCGGAAATCC AGGACCCTTG GGAAGGAAGG GACACAAGGG CCATCCTGGA 
GCAGCAGGGC ACCCTGGAGA ACAGGGGCAG CCAGGACCTG AGGGCAGTCC AGGGGCCAAA 
GGTTACCCTG GCAGGCAGGG GTTCCCTGGA CCTGTAGGAG ACCCTGGCCC CAAAGGCAGC 
AGGGGTTACA TTGGTCTTCC GGGGCTCTTT GGCCTGCCAG GGTCGGATGG AGAGCGGGGT 
CTCCCTGGCG TTCCTGGCAA GAGGGGCGAG ATGGGTAGGC CGGGCTTCCC TGGAGACTTT 
GGGGAAAGAG GCCCCCCTGG ACTTGATGGC AACCCTGGAG AAATTGGCCT GCCGGGGCCA 
CCAGGAGTGC TCGGGCTCAT TGGTGACACA GGAGCACTGG GCCCAGTTGG CTACCCAGGA 
CCGAAAGGCA TGAAGGGACT GATGGGCGGC GTGGGGGAGC CCGGACTGAA AGGTGATAAG 
GGTGAACAAG GGGTGCCAGG TGTGTCGGGA GATCCTGGCT TCCAAGGAGA CAAGGGGAGT 
CATGGCTTGC CAGGGCTCCC GGGTGGCCGG GGGAAGCCAG GGCCTCTGGG AAAAGCTGGA 
GACAAAGGAT CACTTGGGTT TCCTGGACCT CCTGGACCTG AGGGTTTCCC AGGAGACATT 
GGCCCTCCAG GGGACAATGG CCCTGAAGGC ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG 
CCTGGACCTC CTGGGCAGCT GGGGCCTGAG GGAGATGAAG GACCCATGGG GCCACCAGGA 
GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT 
GGCTCGAAGG GGGAACCAGG GGATCCTGGA CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT 
ATGGGATTCA TTGGTCTTGT CGGAGAGCCT GGAATTGTTG GGGAGAAGGG AGACCGTGGG 
GTGATGGGAC CCCCAGGTGC ACCTGGACCC AAGGGGTCGA TGGGCCATCC TGGAACACCC 
GGTGGTATTG GGAACCCTGG AGAGCCTGGA CCCTGGGGCC CTCCAGGATC TCGAGGCCTG 
CCAGGCATGA GGGGAGCCAA GGGGCACCGG GGCCCTCGAG GACCTGATGG ACCAGCTGGG 
GAGCAGGGAT CCAAGGGCCT GAAGGGTCGT GTAGGACCTC GGGGCAGACC TGGCCAGCCA 
GGACAGCAGG GTGCAGCTGG TGAGCGAGGC CACTCAGGTG CAAAAGGCTT CCTTGGCATC 
CCTGGTCCCT CAGGCCCTCC AGGTGCCAAG GGCCTCCCAG GAGAACCGGG CTCTCAGGGA 
CCTCAGGGGC CAGTTGGTCC TCCAGGAGAA ATGGGGCCCA AGGGACCGCC GGGTGCAGTG 
GGAGAGCCTG GCCTTCCTGG GGACTCCGGA ATGAAGGGTG ACCTCGGACC TCTGGGCCCC 
CCTGGGGAGC AAGGTCTTAT TGGGCAGCGG GGAGAGCCAG GCCTTGAGGG AGACCATGGC 
CCTGTGGGGC CAGATGGGCT GAAGGGCGAC AGGGGCGACC CAGGGCCCGA TGGCGAGCAT 
GGTGAAAAGG GCCAGGAAGG GCTGAAAGGA GAGGATGGGT CCCCTGGGCC CCCGGGCATC 
ACTGGCGTCC CGGGTCGTGA GGGGAAGCCG GGCAAGCAGG GTGAGAAAGG CCAGAGGGGA 
GCTAAGGGTG CCAAGGGCCA CCAAGGATAT CTGGGAGAGA TGGGCATCCC CGGAGAACCT 
GGGCCCCCTG GGACCCCAGG TCCTAAAGGG TCCCGGGGCA CCCTAGGACC AACGGGTGCT 
CCAGGACGGA TGGGAGCCCA AGGAGAACCA GGATTGGCCG GTTATAATGG ACACAAAGGC 
ATCACAGGAC CCCTCGGGCC TCCCGGGCCC AAAGGCGAGA AGGGGGACCA GGGCGAGGAT 
GGCAAGACCG AGGGGCCCCC TGGGCCACCA GGAGATCGGG GTCCTGTGGG TGATCGAGGA 
GACCGTGGGG AGCCAGGCGA CCCTGGATAC CCTGGTCAAG AGGGAGTTCA AGGCCTCCGT 
GGAGAACCGG GCCAGCAGGG ACAGCCTGGG CATCCAGGAC CCCGGGGGCG CCCAGGACCC 
AAAGGATCAA AAGGCGAAGA GGGCCCAAAG GGAAAGCCAG GCAAGGCTGG GCCATCAGGC 
CGGAGGGGGA CCCAGGTGAG TTGCCATAGC TTCTCTGTCA CCTTCTATGT TCTCCTGAGA 
AGGTTTAACC AGCTTCTTCT GTACCTCCAG GGCCTTCAAG GACTGCCAGG CCCCCGAGGC 
GTGGTGGGGA GACAGGGCCC TGAGGGCACT GCTGGATCTG ATGGGATTCC TGGCAGAGAT 
GGTCGGCCAG GATATCAGGG AGACCAGGGA AATGATGGGG ACCCTGGCCC TGTGGGCCCT 
GCTGGGAGAA GAGGAAATCC AGGTGTGGCT GGCTTGCCTG GAGCACAGGG GCCTCCGGGA 
TTCAAGGGTG AAAGTGGGTT ACCTGGGCAA CTGGGTCCCC CTGGAAAACG AGGGACAGAA 
GGTGGAACAG GGCTTCCTGG GAACCAGGGG GAGCCAGGAT CCAAAGGCCA GCCGGGTGAC 
TCTGGCGAGA TGGGCTTCCC AGGAGTGGCT GGCCTCTTTG GACCCAAGGG CCCCCCTGGA 
GACATTGGCT TCAAAGGCAT CCAAGGTCCT CGGGGGCCTC CTGGCTTGAT GGGAAAGGAA 
GGTATCATTG GCCCCCCCGG AATGCTGGGA CCTTCTGGAC TCCCGGGTCC CAAAGGTGAC 
AGAGGCAGCC GAGGAGACTT GGGACTGCAA GGCCCAAGGG GTCCTCCTGG TCCAAGGGGC 
CGGCCAGGTC CCCCGGGCCC TCCTTGGCAT CCCATCCAGT TTCAGCAAGA TGACCTTGGA 
GCAGCTTTCC AGACATGGAT GGATGCTCAA GGAGCCGTCA GATCGGAGGG GTACAGCTAT 
CCGGACCAGC TGGCGCTAGA CCAGGGAGGG GAGATCTTCA AAACCTTACA CTACCTCAGC 
AACCTCATCC AGAGCATTAA GACACCCTTG GGCACCAAGG AGAACCCAGC CCGGGTCTGC 
CGGGACCTCA TGGACTGTGA GCAGAGGATG GCGGATGGTA CCTACTGGGT GGACCCCAAC 
CTCGGCTGCT CCTCTGACAC CATTGAAGTC TCCTGCAACT TTACACAGGG TGGGCAGACG 
TGCCTGAAGC CCATCACGGC CTCCAAGGCC GAGTTTGCTG TGAGTCGGGT CCAGATGAAT 
TTCTTGCACC TGCTGAGCTC TGAGGGGACA CAGCATATCA CAATCCACTG TCTGAACATG 
ACGGTGTGGC AGGAGGGACC GGGACGCTCC TCTGCCAGAC AGGCTGTGCG CTTCCGTGCC 
TGGAACGGAC AGGTCTTCGA AGCTGGGGGT CAGTTCAGGC CAGAGGTGTC TATGGATGGC 
TGCAAGGTCC ATGATGGCCG CTGGCATCAG ACACTGTTCA CCTTCCGGAC CCAGGACCCC 
CAGCAGCTGC CCATCGTCAG TGTGGACAAT CTCCCGCCTG TCTCATCAGG GAAGCAGTAC 
CGCCTGGAAG TTGGACCTGC GTGCTTCCTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175773.1
CDS	507..6119
Translation	MGTGFARGARGTAASGPGGGFLFAWILVSFTCHLASTQGAPEDVDVLQRLGLSWTKAGGGRSPTPPGVIPFPSGFIFTQRAKLQAPTANVLPTTLGRELALVLSLCSHRVNHAFLFAIRSRKHKLQLGLQFLPGRTIIHLGPRQSVAFDLDVHDGRWHHLALELRGRTVTMVTACGQHRVPVPLPSRRDSMLDPQGSFLLGKVNPRAVQFEGALCQFSIHPVAQVAHNYCAHLRERCRQVDTYSPQVGTLFPWDSGPAFALHPEPALLGLGNLTRTPATLGARPVSRALAVTLAPAMPTKPLRTVHPDVSEHSSSQTPLSPAKQSARKTPSPSSSASLANSTRVYRPAAAQPRQITTTSPTKRSPTKPSVSPLSVTPMKSPHATQKTGVPSFTKPVPPTQKPAPFTSYLAPSKASSPTVRPVQKTFMTPRPPVPSPQPLRPTTGLSKKFTNPTVAKSKSKTTSWASKPVLARSSVPKTLQQTVLSQSPVSYLGSQTLAPALPPLGVGNPRTMPPTRDSALTPAGSKKFTGRETSKKTRQKSSPRKPEPLSPGKSARDASPRDLTTKPSRPSTPALVLAPAYLLSSSPQPTSSSFPFFHLLGPTPFPMLMGPPGSKGDCGLPGPPGLPGLPGSPGARGPRGPPGPYGNPGPPGPPGAKGQKGDPGLSPGQAHDGAKGNMGLPGLSGNPGPLGRKGHKGHPGAAGHPGEQGQPGPEGSPGAKGYPGRQGFPGPVGDPGPKGSRGYIGLPGLFGLPGSDGERGLPGVPGKRGEMGRPGFPGDFGERGPPGLDGNPGEIGLPGPPGVLGLIGDTGALGPVGYPGPKGMKGLMGGVGEPGLKGDKGEQGVPGVSGDPGFQGDKGSHGLPGLPGGRGKPGPLGKAGDKGSLGFPGPPGPEGFPGDIGPPGDNGPEGMKGKPGARGLPGPPGQLGPEGDEGPMGPPGVPGLEGQPGRKGFPGRPGLDGSKGEPGDPGRPGPVGEQGLMGFIGLVGEPGIVGEKGDRGVMGPPGAPGPKGSMGHPGTPGGIGNPGEPGPWGPPGSRGLPGMRGAKGHRGPRGPDGPAGEQGSKGLKGRVGPRGRPGQPGQQGAAGERGHSGAKGFLGIPGPSGPPGAKGLPGEPGSQGPQGPVGPPGEMGPKGPPGAVGEPGLPGDSGMKGDLGPLGPPGEQGLIGQRGEPGLEGDHGPVGPDGLKGDRGDPGPDGEHGEKGQEGLKGEDGSPGPPGITGVPGREGKPGKQGEKGQRGAKGAKGHQGYLGEMGIPGEPGPPGTPGPKGSRGTLGPTGAPGRMGAQGEPGLAGYNGHKGITGPLGPPGPKGEKGDQGEDGKTEGPPGPPGDRGPVGDRGDRGEPGDPGYPGQEGVQGLRGEPGQQGQPGHPGPRGRPGPKGSKGEEGPKGKPGKAGPSGRRGTQVSCHSFSVTFYVLLRRFNQLLLYLQGLQGLPGPRGVVGRQGPEGTAGSDGIPGRDGRPGYQGDQGNDGDPGPVGPAGRRGNPGVAGLPGAQGPPGFKGESGLPGQLGPPGKRGTEGGTGLPGNQGEPGSKGQPGDSGEMGFPGVAGLFGPKGPPGDIGFKGIQGPRGPPGLMGKEGIIGPPGMLGPSGLPGPKGDRGSRGDLGLQGPRGPPGPRGRPGPPGPPWHPIQFQQDDLGAAFQTWMDAQGAVRSEGYSYPDQLALDQGGEIFKTLHYLSNLIQSIKTPLGTKENPARVCRDLMDCEQRMADGTYWVDPNLGCSSDTIEVSCNFTQGGQTCLKPITASKAEFAVSRVQMNFLHLLSSEGTQHITIHCLNMTVWQEGPGRSSARQAVRFRAWNGQVFEAGGQFRPEVSMDGCKVHDGRWHQTLFTFRTQDPQQLPIVSVDNLPPVSSGKQYRLEVGPACFL

Target ORF information:

RefSeq Version	XM_017320284.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320284.1

ORF Insert Sequence:

ATGGGCACGG GATTCGCGCG GGGGGCCCGA GGCACAGCGG CGTCAGGACC CGGGGGGGGG 
TTTCTCTTCG CCTGGATCTT GGTCTCATTT ACCTGTCACC TGGCCTCCAC CCAAGGAGCT 
CCTGAAGATG TGGATGTCCT CCAGCGGCTG GGCCTCAGCT GGACGAAGGC CGGGGGTGGC 
CGGAGTCCTA CACCCCCTGG TGTCATTCCT TTCCCATCTG GCTTCATCTT CACACAGCGG 
GCCAAGCTGC AGGCCCCCAC AGCCAATGTT CTTCCCACCA CCCTGGGGCG GGAGCTGGCA 
TTGGTGTTGA GCCTCTGTTC ACATCGTGTG AACCATGCCT TCCTCTTTGC CATCCGCAGC 
AGGAAACACA AGCTGCAACT GGGTCTGCAG TTCCTCCCCG GCAGGACGAT CATCCACCTG 
GGGCCTCGGC AATCAGTGGC CTTCGACTTG GATGTGCATG ATGGGCGCTG GCACCACCTG 
GCCCTAGAGT TGCGTGGCCG CACAGTCACA ATGGTGACAG CCTGTGGGCA GCACAGGGTA 
CCTGTCCCAC TGCCTTCCCG TAGGGACTCC ATGCTTGACC CCCAGGGCTC CTTTCTCTTG 
GGGAAGGTGA ACCCCCGTGC TGTCCAGTTT GAAGGTGCAC TCTGTCAGTT CAGCATCCAC 
CCTGTGGCGC AGGTCGCTCA CAATTACTGT GCCCACCTGA GAGAGCGGTG CCGACAGGTG 
GACACATATA GTCCCCAGGT GGGAACCCTC TTTCCTTGGG ACTCTGGCCC AGCCTTTGCT 
TTGCATCCTG AACCAGCCTT GCTTGGCTTG GGGAATCTGA CCAGAACTCC AGCAACCCTG 
GGGGCCAGGC CTGTAAGCAG GGCACTTGCA GTGACTCTGG CTCCTGCCAT GCCCACCAAG 
CCCCTGAGGA CTGTCCACCC AGATGTCTCT GAACACAGCT CTTCCCAGAC CCCACTGTCA 
CCTGCCAAAC AGTCGGCCAG GAAAACACCT TCCCCCTCCT CGTCAGCTTC CCTGGCTAAT 
TCCACCAGGG TTTATCGTCC AGCTGCTGCC CAGCCAAGAC AGATCACAAC TACTAGCCCC 
ACCAAACGTT CCCCCACCAA GCCTTCTGTT TCTCCCCTTT CAGTTACCCC CATGAAAAGT 
CCCCATGCAA CCCAGAAAAC AGGTGTCCCT TCATTCACAA AGCCTGTTCC ACCCACTCAG 
AAGCCAGCGC CCTTCACCTC CTACCTAGCA CCTTCCAAAG CCTCTAGTCC CACTGTGAGG 
CCTGTTCAGA AGACTTTCAT GACACCCCGA CCTCCAGTCC CCAGCCCCCA GCCCCTGCGC 
CCTACTACTG GCTTATCTAA GAAGTTCACT AACCCCACTG TTGCAAAATC TAAGTCCAAG 
ACGACCAGTT GGGCCAGCAA GCCGGTCTTG GCCCGCTCCA GTGTCCCTAA AACGCTTCAG 
CAAACTGTTT TGTCCCAGTC TCCTGTTTCC TATCTGGGCT CTCAGACACT GGCTCCAGCC 
CTCCCTCCTT TGGGTGTTGG AAATCCTAGA ACGATGCCTC CCACACGTGA CTCTGCTTTA 
ACTCCTGCTG GAAGCAAGAA ATTCACAGGA CGGGAAACCT CCAAGAAAAC CAGACAGAAG 
AGCAGCCCCC GGAAGCCCGA ACCTCTCAGC CCTGGGAAGT CAGCCAGGGA TGCCTCACCA 
AGAGACCTGA CAACCAAGCC TAGCCGGCCG TCCACCCCAG CCCTGGTCCT TGCCCCAGCT 
TACCTCCTGT CCTCCAGCCC CCAGCCCACC AGCAGCAGCT TCCCTTTCTT CCACCTGCTG 
GGCCCTACAC CTTTCCCGAT GCTGATGGGG CCTCCAGGCT CCAAGGGAGA CTGTGGCTTG 
CCGGGTCCCC CTGGGCTTCC AGGATTACCT GGATCTCCGG GCGCACGAGG TCCTCGGGGT 
CCTCCTGGGC CGTACGGGAA CCCAGGCCCA CCTGGCCCTC CAGGAGCCAA AGGACAGAAA 
GGGGACCCAG GACTCTCACC AGGACAGGCT CACGATGGAG CAAAGGGCAA CATGGGCTTG 
CCTGGGCTCT CCGGAAATCC AGGACCCTTG GGAAGGAAGG GACACAAGGG CCATCCTGGA 
GCAGCAGGGC ACCCTGGAGA ACAGGGGCAG CCAGGACCTG AGGGCAGTCC AGGGGCCAAA 
GGTTACCCTG GCAGGCAGGG GTTCCCTGGA CCTGTAGGAG ACCCTGGCCC CAAAGGCAGC 
AGGGGTTACA TTGGTCTTCC GGGGCTCTTT GGCCTGCCAG GGTCGGATGG AGAGCGGGGT 
CTCCCTGGCG TTCCTGGCAA GAGGGGCGAG ATGGGTAGGC CGGGCTTCCC TGGAGACTTT 
GGGGAAAGAG GCCCCCCTGG ACTTGATGGC AACCCTGGAG AAATTGGCCT GCCGGGGCCA 
CCAGGAGTGC TCGGGCTCAT TGGTGACACA GGAGCACTGG GCCCAGTTGG CTACCCAGGA 
CCGAAAGGCA TGAAGGGACT GATGGGCGGC GTGGGGGAGC CCGGACTGAA AGGTGATAAG 
GGTGAACAAG GGGTGCCAGG TGTGTCGGGA GATCCTGGCT TCCAAGGAGA CAAGGGGAGT 
CATGGCTTGC CAGGGCTCCC GGGTGGCCGG GGGAAGCCAG GGCCTCTGGG AAAAGCTGGA 
GACAAAGGAT CACTTGGGTT TCCTGGACCT CCTGGACCTG AGGGTTTCCC AGGAGACATT 
GGCCCTCCAG GGGACAATGG CCCTGAAGGC ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG 
CCTGGACCTC CTGGGCAGCT GGGGCCTGAG GGAGATGAAG GACCCATGGG GCCACCAGGA 
GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT 
GGCTCGAAGG GGGAACCAGG GGATCCTGGA CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT 
ATGGGATTCA TTGGTCTTGT CGGAGAGCCT GGAATTGTTG GGGAGAAGGG AGACCGTGGG 
GTGATGGGAC CCCCAGGTGC ACCTGGACCC AAGGGGTCGA TGGGCCATCC TGGAACACCC 
GGTGGTATTG GGAACCCTGG AGAGCCTGGA CCCTGGGGCC CTCCAGGATC TCGAGGCCTG 
CCAGGCATGA GGGGAGCCAA GGGGCACCGG GGCCCTCGAG GACCTGATGG ACCAGCTGGG 
GAGCAGGGAT CCAAGGGCCT GAAGGGTCGT GTAGGACCTC GGGGCAGACC TGGCCAGCCA 
GGACAGCAGG GTGCAGCTGG TGAGCGAGGC CACTCAGGTG CAAAAGGCTT CCTTGGCATC 
CCTGGTCCCT CAGGCCCTCC AGGTGCCAAG GGCCTCCCAG GAGAACCGGG CTCTCAGGGA 
CCTCAGGGGC CAGTTGGTCC TCCAGGAGAA ATGGGGCCCA AGGGACCGCC GGGTGCAGTG 
GGAGAGCCTG GCCTTCCTGG GGACTCCGGA ATGAAGGGTG ACCTCGGACC TCTGGGCCCC 
CCTGGGGAGC AAGGTCTTAT TGGGCAGCGG GGAGAGCCAG GCCTTGAGGG AGACCATGGC 
CCTGTGGGGC CAGATGGGCT GAAGGGCGAC AGGGGCGACC CAGGGCCCGA TGGCGAGCAT 
GGTGAAAAGG GCCAGGAAGG GCTGAAAGGA GAGGATGGGT CCCCTGGGCC CCCGGGCATC 
ACTGGCGTCC CGGGTCGTGA GGGGAAGCCG GGCAAGCAGG GTGAGAAAGG CCAGAGGGGA 
GCTAAGGGTG CCAAGGGCCA CCAAGGATAT CTGGGAGAGA TGGGCATCCC CGGAGAACCT 
GGGCCCCCTG GGACCCCAGG TCCTAAAGGG TCCCGGGGCA CCCTAGGACC AACGGGTGCT 
CCAGGACGGA TGGGAGCCCA AGGAGAACCA GGATTGGCCG GTTATAATGG ACACAAAGGC 
ATCACAGGAC CCCTCGGGCC TCCCGGGCCC AAAGGCGAGA AGGGGGACCA GGGCGAGGAT 
GGCAAGACCG AGGGGCCCCC TGGGCCACCA GGAGATCGGG GTCCTGTGGG TGATCGAGGA 
GACCGTGGGG AGCCAGGCGA CCCTGGATAC CCTGGTCAAG AGGGAGTTCA AGGCCTCCGT 
GGAGAACCGG GCCAGCAGGG ACAGCCTGGG CATCCAGGAC CCCGGGGGCG CCCAGGACCC 
AAAGGATCAA AAGGCGAAGA GGGCCCAAAG GGAAAGCCAG GCAAGGCTGG GCCATCAGGC 
CGGAGGGGGA CCCAGGTGAG TTGCCATAGC TTCTCTGTCA CCTTCTATGT TCTCCTGAGA 
AGGTTTAACC AGCTTCTTCT GTACCTCCAG GGCCTTCAAG GACTGCCAGG CCCCCGAGGC 
GTGGTGGGGA GACAGGGCCC TGAGGGCACT GCTGGATCTG ATGGGATTCC TGGCAGAGAT 
GGTCGGCCAG GATATCAGGG AGACCAGGGA AATGATGGGG ACCCTGGCCC TGTGGGCCCT 
GCTGGGAGAA GAGGAAATCC AGGTGTGGCT GGCTTGCCTG GAGCACAGGG GCCTCCGGGA 
TTCAAGGGTG AAAGTGGGTT ACCTGGGCAA CTGGGTCCCC CTGGAAAACG AGGGACAGAA 
GGTGGAACAG GGCTTCCTGG GAACCAGGGG GAGCCAGGAT CCAAAGGCCA GCCGGGTGAC 
TCTGGCGAGA TGGGCTTCCC AGGAGTGGCT GGCCTCTTTG GACCCAAGGG CCCCCCTGGA 
GACATTGGCT TCAAAGGCAT CCAAGGTCCT CGGGGGCCTC CTGGCTTGAT GGGAAAGGAA 
GGTATCATTG GCCCCCCCGG AATGCTGGGA CCTTCTGGAC TCCCGGGTCC CAAAGGTGAC 
AGAGGCAGCC GAGGAGACTT GGGACTGCAA GGCCCAAGGG GTCCTCCTGG TCCAAGGGGC 
CGGCCAGGTC CCCCGGGCCC TCCTTGGCAT CCCATCCAGT TTCAGCAAGA TGACCTTGGA 
GCAGCTTTCC AGACATGGAT GGATGCTCAA GGAGCCGTCA GATCGGAGGG GTACAGCTAT 
CCGGACCAGC TGGCGCTAGA CCAGGGAGGG GAGATCTTCA AAACCTTACA CTACCTCAGC 
AACCTCATCC AGAGCATTAA GACACCCTTG GGCACCAAGG AGAACCCAGC CCGGGTCTGC 
CGGGACCTCA TGGACTGTGA GCAGAGGATG GCGGATGGTA CCTACTGGGT GGACCCCAAC 
CTCGGCTGCT CCTCTGACAC CATTGAAGTC TCCTGCAACT TTACACAGGG TGGGCAGACG 
TGCCTGAAGC CCATCACGGC CTCCAAGGCC GAGTTTGCTG TGAGTCGGGT CCAGATGAAT 
TTCTTGCACC TGCTGAGCTC TGAGGGGACA CAGCATATCA CAATCCACTG TCTGAACATG 
ACGGTGTGGC AGGAGGGACC GGGACGCTCC TCTGCCAGAC AGGCTGTGCG CTTCCGTGCC 
TGGAACGGAC AGGTCTTCGA AGCTGGGGGT CAGTTCAGGC CAGAGGTGTC TATGGATGGC 
TGCAAGGTCC ATGATGGCCG CTGGCATCAG ACACTGTTCA CCTTCCGGAC CCAGGACCCC 
CAGCAGCTGC CCATCGTCAG TGTGGACAAT CTCCCGCCTG TCTCATCAGG GAAGCAGTAC 
CGCCTGGAAG TTGGACCTGC GTGCTTCCTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col27a1 ( XM_030253629.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030109489.1 Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X2, mRNA.

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CloneID	OMu88564
Clone ID Related Accession (Same CDS sequence)	XM_030253629.1
Accession Version	XM_030253629.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5541bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	collagen alpha-1(XXVII) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGTTAG GCACCCGCCT AAGAACCGAC CGGCCAGCGT CACACTTGGG GACCCAGGGG 
TTTCTCTTCG CCTGGATCTT GGTCTCATTT ACCTGTCACC TGGCCTCCAC CCAAGGAGCT 
CCTGAAGATG TGGATGTCCT CCAGCGGCTG GGCCTCAGCT GGACGAAGGC CGGGGGTGGC 
CGGAGTCCTA CACCCCCTGG TGTCATTCCT TTCCCATCTG GCTTCATCTT CACACAGCGG 
GCCAAGCTGC AGGCCCCCAC AGCCAATGTT CTTCCCACCA CCCTGGGGCG GGAGCTGGCA 
TTGGTGTTGA GCCTCTGTTC ACATCGTGTG AACCATGCCT TCCTCTTTGC CATCCGCAGC 
AGGAAACACA AGCTGCAACT GGGTCTGCAG TTCCTCCCCG GCAGGACGAT CATCCACCTG 
GGGCCTCGGC AATCAGTGGC CTTCGACTTG GATGTGCATG ATGGGCGCTG GCACCACCTG 
GCCCTAGAGT TGCGTGGCCG CACAGTCACA ATGGTGACAG CCTGTGGGCA GCACAGGGTA 
CCTGTCCCAC TGCCTTCCCG TAGGGACTCC ATGCTTGACC CCCAGGGCTC CTTTCTCTTG 
GGGAAGGTGA ACCCCCGTGC TGTCCAGTTT GAAGGTGCAC TCTGTCAGTT CAGCATCCAC 
CCTGTGGCGC AGGTCGCTCA CAATTACTGT GCCCACCTGA GAGAGCGGTG CCGACAGGTG 
GACACATATA GTCCCCAGGT GGGAACCCTC TTTCCTTGGG ACTCTGGCCC AGCCTTTGCT 
TTGCATCCTG AACCAGCCTT GCTTGGCTTG GGGAATCTGA CCAGAACTCC AGCAACCCTG 
GGGGCCAGGC CTGTAAGCAG GGCACTTGCA GTGACTCTGG CTCCTGCCAT GCCCACCAAG 
CCCCTGAGGA CTGTCCACCC AGATGTCTCT GAACACAGCT CTTCCCAGAC CCCACTGTCA 
CCTGCCAAAC AGTCGGCCAG GAAAACACCT TCCCCCTCCT CGTCAGCTTC CCTGGCTAAT 
TCCACCAGGG TTTATCGTCC AGCTGCTGCC CAGCCAAGAC AGATCACAAC TACTAGCCCC 
ACCAAACGTT CCCCCACCAA GCCTTCTGTT TCTCCCCTTT CAGTTACCCC CATGAAAAGT 
CCCCATGCAA CCCAGAAAAC AGGTGTCCCT TCATTCACAA AGCCTGTTCC ACCCACTCAG 
AAGCCAGCGC CCTTCACCTC CTACCTAGCA CCTTCCAAAG CCTCTAGTCC CACTGTGAGG 
CCTGTTCAGA AGACTTTCAT GACACCCCGA CCTCCAGTCC CCAGCCCCCA GCCCCTGCGC 
CCTACTACTG GCTTATCTAA GAAGTTCACT AACCCCACTG TTGCAAAATC TAAGTCCAAG 
ACGACCAGTT GGGCCAGCAA GCCGGTCTTG GCCCGCTCCA GTGTCCCTAA AACGCTTCAG 
CAAACTGTTT TGTCCCAGTC TCCTGTTTCC TATCTGGGCT CTCAGACACT GGCTCCAGCC 
CTCCCTCCTT TGGGTGTTGG AAATCCTAGA ACGATGCCTC CCACACGTGA CTCTGCTTTA 
ACTCCTGCTG GAAGCAAGAA ATTCACAGGA CGGGAAACCT CCAAGAAAAC CAGACAGAAG 
AGCAGCCCCC GGAAGCCCGA ACCTCTCAGC CCTGGGAAGT CAGCCAGGGA TGCCTCACCA 
AGAGACCTGA CAACCAAGCC TAGCCGGCCG TCCACCCCAG CCCTGGTCCT TGCCCCAGCT 
TACCTCCTGT CCTCCAGCCC CCAGCCCACC AGCAGCAGCT TCCCTTTCTT CCACCTGCTG 
GGCCCTACAC CTTTCCCGAT GCTGATGGGG CCTCCAGGCT CCAAGGGAGA CTGTGGCTTG 
CCGGGTCCCC CTGGGCTTCC AGGATTACCT GGATCTCCGG GCGCACGAGG TCCTCGGGGT 
CCTCCTGGGC CGTACGGGAA CCCAGGCCCA CCTGGCCCTC CAGGAGCCAA AGGACAGAAA 
GGGGACCCAG GACTCTCACC AGGACAGGCT CACGATGGAG CAAAGGGCAA CATGGGCTTG 
CCTGGGCTCT CCGGAAATCC AGGACCCTTG GGAAGGAAGG GACACAAGGG CCATCCTGGA 
GCAGCAGGGC ACCCTGGAGA ACAGGGGCAG CCAGGACCTG AGGGCAGTCC AGGGGCCAAA 
GGTTACCCTG GCAGGCAGGG GTTCCCTGGA CCTGTAGGAG ACCCTGGCCC CAAAGGCAGC 
AGGGGTTACA TTGGTCTTCC GGGGCTCTTT GGCCTGCCAG GGTCGGATGG AGAGCGGGGT 
CTCCCTGGCG TTCCTGGCAA GAGGGGCGAG ATGGGTAGGC CGGGCTTCCC TGGAGACTTT 
GGGGAAAGAG GCCCCCCTGG ACTTGATGGC AACCCTGGAG AAATTGGCCT GCCGGGGCCA 
CCAGGAGTGC TCGGGCTCAT TGGTGACACA GGAGCACTGG GCCCAGTTGG CTACCCAGGA 
CCGAAAGGCA TGAAGGGACT GATGGGCGGC GTGGGGGAGC CCGGACTGAA AGGTGATAAG 
GGTGAACAAG GGGTGCCAGG TGTGTCGGGA GATCCTGGCT TCCAAGGAGA CAAGGGGAGT 
CATGGCTTGC CAGGGCTCCC GGGTGGCCGG GGGAAGCCAG GGCCTCTGGG AAAAGCTGGA 
GACAAAGGAT CACTTGGGTT TCCTGGACCT CCTGGACCTG AGGGTTTCCC AGGAGACATT 
GGCCCTCCAG GGGACAATGG CCCTGAAGGC ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG 
CCTGGACCTC CTGGGCAGCT GGGGCCTGAG GGAGATGAAG GACCCATGGG GCCACCAGGA 
GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT 
GGCTCGAAGG GGGAACCAGG GGATCCTGGA CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT 
ATGGGATTCA TTGGTCTTGT CGGAGAGCCT GGAATTGTTG GGGAGAAGGG AGACCGTGGG 
GTGATGGGAC CCCCAGGTGC ACCTGGACCC AAGGGGTCGA TGGGCCATCC TGGAACACCC 
GGTGGTATTG GGAACCCTGG AGAGCCTGGA CCCTGGGGCC CTCCAGGATC TCGAGGCCTG 
CCAGGCATGA GGGGAGCCAA GGGGCACCGG GGCCCTCGAG GACCTGATGG ACCAGCTGGG 
GAGCAGGGAT CCAAGGGCCT GAAGGGTCGT GTAGGACCTC GGGGCAGACC TGGCCAGCCA 
GGACAGCAGG GTGCAGCTGG TGAGCGAGGC CACTCAGGTG CAAAAGGCTT CCTTGGCATC 
CCTGGTCCCT CAGGCCCTCC AGGTGCCAAG GGCCTCCCAG GAGAACCGGG CTCTCAGGGA 
CCTCAGGGGC CAGTTGGTCC TCCAGGAGAA ATGGGGCCCA AGGGACCGCC GGGTGCAGTG 
GGAGAGCCTG GCCTTCCTGG GGACTCCGGA ATGAAGGGTG ACCTCGGACC TCTGGGCCCC 
CCTGGGGAGC AAGGTCTTAT TGGGCAGCGG GGAGAGCCAG GCCTTGAGGG AGACCATGGC 
CCTGTGGGGC CAGATGGGCT GAAGGGCGAC AGGGGCGACC CAGGGCCCGA TGGCGAGCAT 
GGTGAAAAGG GCCAGGAAGG GCTGAAAGGA GAGGATGGGT CCCCTGGGCC CCCGGGCATC 
ACTGGCGTCC CGGGTCGTGA GGGGAAGCCG GGCAAGCAGG GTGAGAAAGG CCAGAGGGGA 
GCTAAGGGTG CCAAGGGCCA CCAAGGATAT CTGGGAGAGA TGGGCATCCC CGGAGAACCT 
GGGCCCCCTG GGACCCCAGG TCCTAAAGGG TCCCGGGGCA CCCTAGGACC AACGGGTGCT 
CCAGGACGGA TGGGAGCCCA AGGAGAACCA GGATTGGCCG GTTATAATGG ACACAAAGGC 
ATCACAGGAC CCCTCGGGCC TCCCGGGCCC AAAGGCGAGA AGGGGGACCA GGGCGAGGAT 
GGCAAGACCG AGGGGCCCCC TGGGCCACCA GGAGATCGGG GTCCTGTGGG TGATCGAGGA 
GACCGTGGGG AGCCAGGCGA CCCTGGATAC CCTGGTCAAG AGGGAGTTCA AGGCCTCCGT 
GGAGAACCGG GCCAGCAGGG ACAGCCTGGG CATCCAGGAC CCCGGGGGCG CCCAGGACCC 
AAAGGATCAA AAGGCGAAGA GGGCCCAAAG GGAAAGCCAG GCAAGGCTGG GCCATCAGGC 
CGGAGGGGGA CCCAGGGCCT TCAAGGACTG CCAGGCCCCC GAGGCGTGGT GGGGAGACAG 
GGCCCTGAGG GCACTGCTGG ATCTGATGGG ATTCCTGGCA GAGATGGTCG GCCAGGATAT 
CAGGGAGACC AGGGAAATGA TGGGGACCCT GGCCCTGTGG GCCCTGCTGG GAGAAGAGGA 
AATCCAGGTG TGGCTGGCTT GCCTGGAGCA CAGGGGCCTC CGGGATTCAA GGGTGAAAGT 
GGGTTACCTG GGCAACTGGG TCCCCCTGGA AAACGAGGGA CAGAAGGTGG AACAGGGCTT 
CCTGGGAACC AGGGGGAGCC AGGATCCAAA GGCCAGCCGG GTGACTCTGG CGAGATGGGC 
TTCCCAGGAG TGGCTGGCCT CTTTGGACCC AAGGGCCCCC CTGGAGACAT TGGCTTCAAA 
GGCATCCAAG GTCCTCGGGG GCCTCCTGGC TTGATGGGAA AGGAAGGTAT CATTGGCCCC 
CCCGGAATGC TGGGACCTTC TGGACTCCCG GGTCCCAAAG GTGACAGAGG CAGCCGAGGA 
GACTTGGGAC TGCAAGGCCC AAGGGGTCCT CCTGGTCCAA GGGGCCGGCC AGGTCCCCCG 
GGCCCTCCTT GGCATCCCAT CCAGTTTCAG CAAGATGACC TTGGAGCAGC TTTCCAGACA 
TGGATGGATG CTCAAGGAGC CGTCAGATCG GAGCAGGGGT ACAGCTATCC GGACCAGCTG 
GCGCTAGACC AGGGAGGGGA GATCTTCAAA ACCTTACACT ACCTCAGCAA CCTCATCCAG 
AGCATTAAGA CACCCTTGGG CACCAAGGAG AACCCAGCCC GGGTCTGCCG GGACCTCATG 
GACTGTGAGC AGAGGATGGC GGATGGTACC TACTGGGTGG ACCCCAACCT CGGCTGCTCC 
TCTGACACCA TTGAAGTCTC CTGCAACTTT ACACAGGGTG GGCAGACGTG CCTGAAGCCC 
ATCACGGCCT CCAAGGCCGA GTTTGCTGTG AGTCGGGTCC AGATGAATTT CTTGCACCTG 
CTGAGCTCTG AGGGGACACA GCATATCACA ATCCACTGTC TGAACATGAC GGTGTGGCAG 
GAGGGACCGG GACGCTCCTC TGCCAGACAG GCTGTGCGCT TCCGTGCCTG GAACGGACAG 
GTCTTCGAAG CTGGGGGTCA GTTCAGGCCA GAGGTGTCTA TGGATGGCTG CAAGGTCCAT 
GATGGCCGCT GGCATCAGAC ACTGTTCACC TTCCGGACCC AGGACCCCCA GCAGCTGCCC 
ATCGTCAGTG TGGACAATCT CCCGCCTGTC TCATCAGGGA AGCAGTACCG CCTGGAAGTT 
GGACCTGCGT GCTTCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030109489.1
CDS	341..5881
Translation	MPLGTRLRTDRPASHLGTQGFLFAWILVSFTCHLASTQGAPEDVDVLQRLGLSWTKAGGGRSPTPPGVIPFPSGFIFTQRAKLQAPTANVLPTTLGRELALVLSLCSHRVNHAFLFAIRSRKHKLQLGLQFLPGRTIIHLGPRQSVAFDLDVHDGRWHHLALELRGRTVTMVTACGQHRVPVPLPSRRDSMLDPQGSFLLGKVNPRAVQFEGALCQFSIHPVAQVAHNYCAHLRERCRQVDTYSPQVGTLFPWDSGPAFALHPEPALLGLGNLTRTPATLGARPVSRALAVTLAPAMPTKPLRTVHPDVSEHSSSQTPLSPAKQSARKTPSPSSSASLANSTRVYRPAAAQPRQITTTSPTKRSPTKPSVSPLSVTPMKSPHATQKTGVPSFTKPVPPTQKPAPFTSYLAPSKASSPTVRPVQKTFMTPRPPVPSPQPLRPTTGLSKKFTNPTVAKSKSKTTSWASKPVLARSSVPKTLQQTVLSQSPVSYLGSQTLAPALPPLGVGNPRTMPPTRDSALTPAGSKKFTGRETSKKTRQKSSPRKPEPLSPGKSARDASPRDLTTKPSRPSTPALVLAPAYLLSSSPQPTSSSFPFFHLLGPTPFPMLMGPPGSKGDCGLPGPPGLPGLPGSPGARGPRGPPGPYGNPGPPGPPGAKGQKGDPGLSPGQAHDGAKGNMGLPGLSGNPGPLGRKGHKGHPGAAGHPGEQGQPGPEGSPGAKGYPGRQGFPGPVGDPGPKGSRGYIGLPGLFGLPGSDGERGLPGVPGKRGEMGRPGFPGDFGERGPPGLDGNPGEIGLPGPPGVLGLIGDTGALGPVGYPGPKGMKGLMGGVGEPGLKGDKGEQGVPGVSGDPGFQGDKGSHGLPGLPGGRGKPGPLGKAGDKGSLGFPGPPGPEGFPGDIGPPGDNGPEGMKGKPGARGLPGPPGQLGPEGDEGPMGPPGVPGLEGQPGRKGFPGRPGLDGSKGEPGDPGRPGPVGEQGLMGFIGLVGEPGIVGEKGDRGVMGPPGAPGPKGSMGHPGTPGGIGNPGEPGPWGPPGSRGLPGMRGAKGHRGPRGPDGPAGEQGSKGLKGRVGPRGRPGQPGQQGAAGERGHSGAKGFLGIPGPSGPPGAKGLPGEPGSQGPQGPVGPPGEMGPKGPPGAVGEPGLPGDSGMKGDLGPLGPPGEQGLIGQRGEPGLEGDHGPVGPDGLKGDRGDPGPDGEHGEKGQEGLKGEDGSPGPPGITGVPGREGKPGKQGEKGQRGAKGAKGHQGYLGEMGIPGEPGPPGTPGPKGSRGTLGPTGAPGRMGAQGEPGLAGYNGHKGITGPLGPPGPKGEKGDQGEDGKTEGPPGPPGDRGPVGDRGDRGEPGDPGYPGQEGVQGLRGEPGQQGQPGHPGPRGRPGPKGSKGEEGPKGKPGKAGPSGRRGTQGLQGLPGPRGVVGRQGPEGTAGSDGIPGRDGRPGYQGDQGNDGDPGPVGPAGRRGNPGVAGLPGAQGPPGFKGESGLPGQLGPPGKRGTEGGTGLPGNQGEPGSKGQPGDSGEMGFPGVAGLFGPKGPPGDIGFKGIQGPRGPPGLMGKEGIIGPPGMLGPSGLPGPKGDRGSRGDLGLQGPRGPPGPRGRPGPPGPPWHPIQFQQDDLGAAFQTWMDAQGAVRSEQGYSYPDQLALDQGGEIFKTLHYLSNLIQSIKTPLGTKENPARVCRDLMDCEQRMADGTYWVDPNLGCSSDTIEVSCNFTQGGQTCLKPITASKAEFAVSRVQMNFLHLLSSEGTQHITIHCLNMTVWQEGPGRSSARQAVRFRAWNGQVFEAGGQFRPEVSMDGCKVHDGRWHQTLFTFRTQDPQQLPIVSVDNLPPVSSGKQYRLEVGPACFL

Target ORF information:

RefSeq Version	XM_030253629.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030253629.1

ORF Insert Sequence:

ATGCCGTTAG GCACCCGCCT AAGAACCGAC CGGCCAGCGT CACACTTGGG GACCCAGGGG 
TTTCTCTTCG CCTGGATCTT GGTCTCATTT ACCTGTCACC TGGCCTCCAC CCAAGGAGCT 
CCTGAAGATG TGGATGTCCT CCAGCGGCTG GGCCTCAGCT GGACGAAGGC CGGGGGTGGC 
CGGAGTCCTA CACCCCCTGG TGTCATTCCT TTCCCATCTG GCTTCATCTT CACACAGCGG 
GCCAAGCTGC AGGCCCCCAC AGCCAATGTT CTTCCCACCA CCCTGGGGCG GGAGCTGGCA 
TTGGTGTTGA GCCTCTGTTC ACATCGTGTG AACCATGCCT TCCTCTTTGC CATCCGCAGC 
AGGAAACACA AGCTGCAACT GGGTCTGCAG TTCCTCCCCG GCAGGACGAT CATCCACCTG 
GGGCCTCGGC AATCAGTGGC CTTCGACTTG GATGTGCATG ATGGGCGCTG GCACCACCTG 
GCCCTAGAGT TGCGTGGCCG CACAGTCACA ATGGTGACAG CCTGTGGGCA GCACAGGGTA 
CCTGTCCCAC TGCCTTCCCG TAGGGACTCC ATGCTTGACC CCCAGGGCTC CTTTCTCTTG 
GGGAAGGTGA ACCCCCGTGC TGTCCAGTTT GAAGGTGCAC TCTGTCAGTT CAGCATCCAC 
CCTGTGGCGC AGGTCGCTCA CAATTACTGT GCCCACCTGA GAGAGCGGTG CCGACAGGTG 
GACACATATA GTCCCCAGGT GGGAACCCTC TTTCCTTGGG ACTCTGGCCC AGCCTTTGCT 
TTGCATCCTG AACCAGCCTT GCTTGGCTTG GGGAATCTGA CCAGAACTCC AGCAACCCTG 
GGGGCCAGGC CTGTAAGCAG GGCACTTGCA GTGACTCTGG CTCCTGCCAT GCCCACCAAG 
CCCCTGAGGA CTGTCCACCC AGATGTCTCT GAACACAGCT CTTCCCAGAC CCCACTGTCA 
CCTGCCAAAC AGTCGGCCAG GAAAACACCT TCCCCCTCCT CGTCAGCTTC CCTGGCTAAT 
TCCACCAGGG TTTATCGTCC AGCTGCTGCC CAGCCAAGAC AGATCACAAC TACTAGCCCC 
ACCAAACGTT CCCCCACCAA GCCTTCTGTT TCTCCCCTTT CAGTTACCCC CATGAAAAGT 
CCCCATGCAA CCCAGAAAAC AGGTGTCCCT TCATTCACAA AGCCTGTTCC ACCCACTCAG 
AAGCCAGCGC CCTTCACCTC CTACCTAGCA CCTTCCAAAG CCTCTAGTCC CACTGTGAGG 
CCTGTTCAGA AGACTTTCAT GACACCCCGA CCTCCAGTCC CCAGCCCCCA GCCCCTGCGC 
CCTACTACTG GCTTATCTAA GAAGTTCACT AACCCCACTG TTGCAAAATC TAAGTCCAAG 
ACGACCAGTT GGGCCAGCAA GCCGGTCTTG GCCCGCTCCA GTGTCCCTAA AACGCTTCAG 
CAAACTGTTT TGTCCCAGTC TCCTGTTTCC TATCTGGGCT CTCAGACACT GGCTCCAGCC 
CTCCCTCCTT TGGGTGTTGG AAATCCTAGA ACGATGCCTC CCACACGTGA CTCTGCTTTA 
ACTCCTGCTG GAAGCAAGAA ATTCACAGGA CGGGAAACCT CCAAGAAAAC CAGACAGAAG 
AGCAGCCCCC GGAAGCCCGA ACCTCTCAGC CCTGGGAAGT CAGCCAGGGA TGCCTCACCA 
AGAGACCTGA CAACCAAGCC TAGCCGGCCG TCCACCCCAG CCCTGGTCCT TGCCCCAGCT 
TACCTCCTGT CCTCCAGCCC CCAGCCCACC AGCAGCAGCT TCCCTTTCTT CCACCTGCTG 
GGCCCTACAC CTTTCCCGAT GCTGATGGGG CCTCCAGGCT CCAAGGGAGA CTGTGGCTTG 
CCGGGTCCCC CTGGGCTTCC AGGATTACCT GGATCTCCGG GCGCACGAGG TCCTCGGGGT 
CCTCCTGGGC CGTACGGGAA CCCAGGCCCA CCTGGCCCTC CAGGAGCCAA AGGACAGAAA 
GGGGACCCAG GACTCTCACC AGGACAGGCT CACGATGGAG CAAAGGGCAA CATGGGCTTG 
CCTGGGCTCT CCGGAAATCC AGGACCCTTG GGAAGGAAGG GACACAAGGG CCATCCTGGA 
GCAGCAGGGC ACCCTGGAGA ACAGGGGCAG CCAGGACCTG AGGGCAGTCC AGGGGCCAAA 
GGTTACCCTG GCAGGCAGGG GTTCCCTGGA CCTGTAGGAG ACCCTGGCCC CAAAGGCAGC 
AGGGGTTACA TTGGTCTTCC GGGGCTCTTT GGCCTGCCAG GGTCGGATGG AGAGCGGGGT 
CTCCCTGGCG TTCCTGGCAA GAGGGGCGAG ATGGGTAGGC CGGGCTTCCC TGGAGACTTT 
GGGGAAAGAG GCCCCCCTGG ACTTGATGGC AACCCTGGAG AAATTGGCCT GCCGGGGCCA 
CCAGGAGTGC TCGGGCTCAT TGGTGACACA GGAGCACTGG GCCCAGTTGG CTACCCAGGA 
CCGAAAGGCA TGAAGGGACT GATGGGCGGC GTGGGGGAGC CCGGACTGAA AGGTGATAAG 
GGTGAACAAG GGGTGCCAGG TGTGTCGGGA GATCCTGGCT TCCAAGGAGA CAAGGGGAGT 
CATGGCTTGC CAGGGCTCCC GGGTGGCCGG GGGAAGCCAG GGCCTCTGGG AAAAGCTGGA 
GACAAAGGAT CACTTGGGTT TCCTGGACCT CCTGGACCTG AGGGTTTCCC AGGAGACATT 
GGCCCTCCAG GGGACAATGG CCCTGAAGGC ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG 
CCTGGACCTC CTGGGCAGCT GGGGCCTGAG GGAGATGAAG GACCCATGGG GCCACCAGGA 
GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT 
GGCTCGAAGG GGGAACCAGG GGATCCTGGA CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT 
ATGGGATTCA TTGGTCTTGT CGGAGAGCCT GGAATTGTTG GGGAGAAGGG AGACCGTGGG 
GTGATGGGAC CCCCAGGTGC ACCTGGACCC AAGGGGTCGA TGGGCCATCC TGGAACACCC 
GGTGGTATTG GGAACCCTGG AGAGCCTGGA CCCTGGGGCC CTCCAGGATC TCGAGGCCTG 
CCAGGCATGA GGGGAGCCAA GGGGCACCGG GGCCCTCGAG GACCTGATGG ACCAGCTGGG 
GAGCAGGGAT CCAAGGGCCT GAAGGGTCGT GTAGGACCTC GGGGCAGACC TGGCCAGCCA 
GGACAGCAGG GTGCAGCTGG TGAGCGAGGC CACTCAGGTG CAAAAGGCTT CCTTGGCATC 
CCTGGTCCCT CAGGCCCTCC AGGTGCCAAG GGCCTCCCAG GAGAACCGGG CTCTCAGGGA 
CCTCAGGGGC CAGTTGGTCC TCCAGGAGAA ATGGGGCCCA AGGGACCGCC GGGTGCAGTG 
GGAGAGCCTG GCCTTCCTGG GGACTCCGGA ATGAAGGGTG ACCTCGGACC TCTGGGCCCC 
CCTGGGGAGC AAGGTCTTAT TGGGCAGCGG GGAGAGCCAG GCCTTGAGGG AGACCATGGC 
CCTGTGGGGC CAGATGGGCT GAAGGGCGAC AGGGGCGACC CAGGGCCCGA TGGCGAGCAT 
GGTGAAAAGG GCCAGGAAGG GCTGAAAGGA GAGGATGGGT CCCCTGGGCC CCCGGGCATC 
ACTGGCGTCC CGGGTCGTGA GGGGAAGCCG GGCAAGCAGG GTGAGAAAGG CCAGAGGGGA 
GCTAAGGGTG CCAAGGGCCA CCAAGGATAT CTGGGAGAGA TGGGCATCCC CGGAGAACCT 
GGGCCCCCTG GGACCCCAGG TCCTAAAGGG TCCCGGGGCA CCCTAGGACC AACGGGTGCT 
CCAGGACGGA TGGGAGCCCA AGGAGAACCA GGATTGGCCG GTTATAATGG ACACAAAGGC 
ATCACAGGAC CCCTCGGGCC TCCCGGGCCC AAAGGCGAGA AGGGGGACCA GGGCGAGGAT 
GGCAAGACCG AGGGGCCCCC TGGGCCACCA GGAGATCGGG GTCCTGTGGG TGATCGAGGA 
GACCGTGGGG AGCCAGGCGA CCCTGGATAC CCTGGTCAAG AGGGAGTTCA AGGCCTCCGT 
GGAGAACCGG GCCAGCAGGG ACAGCCTGGG CATCCAGGAC CCCGGGGGCG CCCAGGACCC 
AAAGGATCAA AAGGCGAAGA GGGCCCAAAG GGAAAGCCAG GCAAGGCTGG GCCATCAGGC 
CGGAGGGGGA CCCAGGGCCT TCAAGGACTG CCAGGCCCCC GAGGCGTGGT GGGGAGACAG 
GGCCCTGAGG GCACTGCTGG ATCTGATGGG ATTCCTGGCA GAGATGGTCG GCCAGGATAT 
CAGGGAGACC AGGGAAATGA TGGGGACCCT GGCCCTGTGG GCCCTGCTGG GAGAAGAGGA 
AATCCAGGTG TGGCTGGCTT GCCTGGAGCA CAGGGGCCTC CGGGATTCAA GGGTGAAAGT 
GGGTTACCTG GGCAACTGGG TCCCCCTGGA AAACGAGGGA CAGAAGGTGG AACAGGGCTT 
CCTGGGAACC AGGGGGAGCC AGGATCCAAA GGCCAGCCGG GTGACTCTGG CGAGATGGGC 
TTCCCAGGAG TGGCTGGCCT CTTTGGACCC AAGGGCCCCC CTGGAGACAT TGGCTTCAAA 
GGCATCCAAG GTCCTCGGGG GCCTCCTGGC TTGATGGGAA AGGAAGGTAT CATTGGCCCC 
CCCGGAATGC TGGGACCTTC TGGACTCCCG GGTCCCAAAG GTGACAGAGG CAGCCGAGGA 
GACTTGGGAC TGCAAGGCCC AAGGGGTCCT CCTGGTCCAA GGGGCCGGCC AGGTCCCCCG 
GGCCCTCCTT GGCATCCCAT CCAGTTTCAG CAAGATGACC TTGGAGCAGC TTTCCAGACA 
TGGATGGATG CTCAAGGAGC CGTCAGATCG GAGCAGGGGT ACAGCTATCC GGACCAGCTG 
GCGCTAGACC AGGGAGGGGA GATCTTCAAA ACCTTACACT ACCTCAGCAA CCTCATCCAG 
AGCATTAAGA CACCCTTGGG CACCAAGGAG AACCCAGCCC GGGTCTGCCG GGACCTCATG 
GACTGTGAGC AGAGGATGGC GGATGGTACC TACTGGGTGG ACCCCAACCT CGGCTGCTCC 
TCTGACACCA TTGAAGTCTC CTGCAACTTT ACACAGGGTG GGCAGACGTG CCTGAAGCCC 
ATCACGGCCT CCAAGGCCGA GTTTGCTGTG AGTCGGGTCC AGATGAATTT CTTGCACCTG 
CTGAGCTCTG AGGGGACACA GCATATCACA ATCCACTGTC TGAACATGAC GGTGTGGCAG 
GAGGGACCGG GACGCTCCTC TGCCAGACAG GCTGTGCGCT TCCGTGCCTG GAACGGACAG 
GTCTTCGAAG CTGGGGGTCA GTTCAGGCCA GAGGTGTCTA TGGATGGCTG CAAGGTCCAT 
GATGGCCGCT GGCATCAGAC ACTGTTCACC TTCCGGACCC AGGACCCCCA GCAGCTGCCC 
ATCGTCAGTG TGGACAATCT CCCGCCTGTC TCATCAGGGA AGCAGTACCG CCTGGAAGTT 
GGACCTGCGT GCTTCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col27a1 ( XM_030253630.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030109490.1 Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X3, mRNA.

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CloneID	OMu88565
Clone ID Related Accession (Same CDS sequence)	XM_030253630.1
Accession Version	XM_030253630.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5031bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	collagen alpha-1(XXVII) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGACAG CCTGTGGGCA GCACAGGGTA CCTGTCCCAC TGCCTTCCCG TAGGGACTCC 
ATGCTTGACC CCCAGGGCTC CTTTCTCTTG GGGAAGGTGA ACCCCCGTGC TGTCCAGTTT 
GAAGGTGCAC TCTGTCAGTT CAGCATCCAC CCTGTGGCGC AGGTCGCTCA CAATTACTGT 
GCCCACCTGA GAGAGCGGTG CCGACAGGTG GACACATATA GTCCCCAGGT GGGAACCCTC 
TTTCCTTGGG ACTCTGGCCC AGCCTTTGCT TTGCATCCTG AACCAGCCTT GCTTGGCTTG 
GGGAATCTGA CCAGAACTCC AGCAACCCTG GGGGCCAGGC CTGTAAGCAG GGCACTTGCA 
GTGACTCTGG CTCCTGCCAT GCCCACCAAG CCCCTGAGGA CTGTCCACCC AGATGTCTCT 
GAACACAGCT CTTCCCAGAC CCCACTGTCA CCTGCCAAAC AGTCGGCCAG GAAAACACCT 
TCCCCCTCCT CGTCAGCTTC CCTGGCTAAT TCCACCAGGG TTTATCGTCC AGCTGCTGCC 
CAGCCAAGAC AGATCACAAC TACTAGCCCC ACCAAACGTT CCCCCACCAA GCCTTCTGTT 
TCTCCCCTTT CAGTTACCCC CATGAAAAGT CCCCATGCAA CCCAGAAAAC AGGTGTCCCT 
TCATTCACAA AGCCTGTTCC ACCCACTCAG AAGCCAGCGC CCTTCACCTC CTACCTAGCA 
CCTTCCAAAG CCTCTAGTCC CACTGTGAGG CCTGTTCAGA AGACTTTCAT GACACCCCGA 
CCTCCAGTCC CCAGCCCCCA GCCCCTGCGC CCTACTACTG GCTTATCTAA GAAGTTCACT 
AACCCCACTG TTGCAAAATC TAAGTCCAAG ACGACCAGTT GGGCCAGCAA GCCGGTCTTG 
GCCCGCTCCA GTGTCCCTAA AACGCTTCAG CAAACTGTTT TGTCCCAGTC TCCTGTTTCC 
TATCTGGGCT CTCAGACACT GGCTCCAGCC CTCCCTCCTT TGGGTGTTGG AAATCCTAGA 
ACGATGCCTC CCACACGTGA CTCTGCTTTA ACTCCTGCTG GAAGCAAGAA ATTCACAGGA 
CGGGAAACCT CCAAGAAAAC CAGACAGAAG AGCAGCCCCC GGAAGCCCGA ACCTCTCAGC 
CCTGGGAAGT CAGCCAGGGA TGCCTCACCA AGAGACCTGA CAACCAAGCC TAGCCGGCCG 
TCCACCCCAG CCCTGGTCCT TGCCCCAGCT TACCTCCTGT CCTCCAGCCC CCAGCCCACC 
AGCAGCAGCT TCCCTTTCTT CCACCTGCTG GGCCCTACAC CTTTCCCGAT GCTGATGGGG 
CCTCCAGGCT CCAAGGGAGA CTGTGGCTTG CCGGGTCCCC CTGGGCTTCC AGGATTACCT 
GGATCTCCGG GCGCACGAGG TCCTCGGGGT CCTCCTGGGC CGTACGGGAA CCCAGGCCCA 
CCTGGCCCTC CAGGAGCCAA AGGACAGAAA GGGGACCCAG GACTCTCACC AGGACAGGCT 
CACGATGGAG CAAAGGGCAA CATGGGCTTG CCTGGGCTCT CCGGAAATCC AGGACCCTTG 
GGAAGGAAGG GACACAAGGG CCATCCTGGA GCAGCAGGGC ACCCTGGAGA ACAGGGGCAG 
CCAGGACCTG AGGGCAGTCC AGGGGCCAAA GGTTACCCTG GCAGGCAGGG GTTCCCTGGA 
CCTGTAGGAG ACCCTGGCCC CAAAGGCAGC AGGGGTTACA TTGGTCTTCC GGGGCTCTTT 
GGCCTGCCAG GGTCGGATGG AGAGCGGGGT CTCCCTGGCG TTCCTGGCAA GAGGGGCGAG 
ATGGGTAGGC CGGGCTTCCC TGGAGACTTT GGGGAAAGAG GCCCCCCTGG ACTTGATGGC 
AACCCTGGAG AAATTGGCCT GCCGGGGCCA CCAGGAGTGC TCGGGCTCAT TGGTGACACA 
GGAGCACTGG GCCCAGTTGG CTACCCAGGA CCGAAAGGCA TGAAGGGACT GATGGGCGGC 
GTGGGGGAGC CCGGACTGAA AGGTGATAAG GGTGAACAAG GGGTGCCAGG TGTGTCGGGA 
GATCCTGGCT TCCAAGGAGA CAAGGGGAGT CATGGCTTGC CAGGGCTCCC GGGTGGCCGG 
GGGAAGCCAG GGCCTCTGGG AAAAGCTGGA GACAAAGGAT CACTTGGGTT TCCTGGACCT 
CCTGGACCTG AGGGTTTCCC AGGAGACATT GGCCCTCCAG GGGACAATGG CCCTGAAGGC 
ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG CCTGGACCTC CTGGGCAGCT GGGGCCTGAG 
GGAGATGAAG GACCCATGGG GCCACCAGGA GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG 
AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT GGCTCGAAGG GGGAACCAGG GGATCCTGGA 
CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT ATGGGATTCA TTGGTCTTGT CGGAGAGCCT 
GGAATTGTTG GGGAGAAGGG AGACCGTGGG GTGATGGGAC CCCCAGGTGC ACCTGGACCC 
AAGGGGTCGA TGGGCCATCC TGGAACACCC GGTGGTATTG GGAACCCTGG AGAGCCTGGA 
CCCTGGGGCC CTCCAGGATC TCGAGGCCTG CCAGGCATGA GGGGAGCCAA GGGGCACCGG 
GGCCCTCGAG GACCTGATGG ACCAGCTGGG GAGCAGGGAT CCAAGGGCCT GAAGGGTCGT 
GTAGGACCTC GGGGCAGACC TGGCCAGCCA GGACAGCAGG GTGCAGCTGG TGAGCGAGGC 
CACTCAGGTG CAAAAGGCTT CCTTGGCATC CCTGGTCCCT CAGGCCCTCC AGGTGCCAAG 
GGCCTCCCAG GAGAACCGGG CTCTCAGGGA CCTCAGGGGC CAGTTGGTCC TCCAGGAGAA 
ATGGGGCCCA AGGGACCGCC GGGTGCAGTG GGAGAGCCTG GCCTTCCTGG GGACTCCGGA 
ATGAAGGGTG ACCTCGGACC TCTGGGCCCC CCTGGGGAGC AAGGTCTTAT TGGGCAGCGG 
GGAGAGCCAG GCCTTGAGGG AGACCATGGC CCTGTGGGGC CAGATGGGCT GAAGGGCGAC 
AGGGGCGACC CAGGGCCCGA TGGCGAGCAT GGTGAAAAGG GCCAGGAAGG GCTGAAAGGA 
GAGGATGGGT CCCCTGGGCC CCCGGGCATC ACTGGCGTCC CGGGTCGTGA GGGGAAGCCG 
GGCAAGCAGG GTGAGAAAGG CCAGAGGGGA GCTAAGGGTG CCAAGGGCCA CCAAGGATAT 
CTGGGAGAGA TGGGCATCCC CGGAGAACCT GGGCCCCCTG GGACCCCAGG TCCTAAAGGG 
TCCCGGGGCA CCCTAGGACC AACGGGTGCT CCAGGACGGA TGGGAGCCCA AGGAGAACCA 
GGATTGGCCG GTTATAATGG ACACAAAGGC ATCACAGGAC CCCTCGGGCC TCCCGGGCCC 
AAAGGCGAGA AGGGGGACCA GGGCGAGGAT GGCAAGACCG AGGGGCCCCC TGGGCCACCA 
GGAGATCGGG GTCCTGTGGG TGATCGAGGA GACCGTGGGG AGCCAGGCGA CCCTGGATAC 
CCTGGTCAAG AGGGAGTTCA AGGCCTCCGT GGAGAACCGG GCCAGCAGGG ACAGCCTGGG 
CATCCAGGAC CCCGGGGGCG CCCAGGACCC AAAGGATCAA AAGGCGAAGA GGGCCCAAAG 
GGAAAGCCAG GCAAGGCTGG GCCATCAGGC CGGAGGGGGA CCCAGGGCCT TCAAGGACTG 
CCAGGCCCCC GAGGCGTGGT GGGGAGACAG GGCCCTGAGG GCACTGCTGG ATCTGATGGG 
ATTCCTGGCA GAGATGGTCG GCCAGGATAT CAGGGAGACC AGGGAAATGA TGGGGACCCT 
GGCCCTGTGG GCCCTGCTGG GAGAAGAGGA AATCCAGGTG TGGCTGGCTT GCCTGGAGCA 
CAGGGGCCTC CGGGATTCAA GGGTGAAAGT GGGTTACCTG GGCAACTGGG TCCCCCTGGA 
AAACGAGGGA CAGAAGGTGG AACAGGGCTT CCTGGGAACC AGGGGGAGCC AGGATCCAAA 
GGCCAGCCGG GTGACTCTGG CGAGATGGGC TTCCCAGGAG TGGCTGGCCT CTTTGGACCC 
AAGGGCCCCC CTGGAGACAT TGGCTTCAAA GGCATCCAAG GTCCTCGGGG GCCTCCTGGC 
TTGATGGGAA AGGAAGGTAT CATTGGCCCC CCCGGAATGC TGGGACCTTC TGGACTCCCG 
GGTCCCAAAG GTGACAGAGG CAGCCGAGGA GACTTGGGAC TGCAAGGCCC AAGGGGTCCT 
CCTGGTCCAA GGGGCCGGCC AGGTCCCCCG GGCCCTCCTT GGCATCCCAT CCAGTTTCAG 
CAAGATGACC TTGGAGCAGC TTTCCAGACA TGGATGGATG CTCAAGGAGC CGTCAGATCG 
GAGCAGGGGT ACAGCTATCC GGACCAGCTG GCGCTAGACC AGGGAGGGGA GATCTTCAAA 
ACCTTACACT ACCTCAGCAA CCTCATCCAG AGCATTAAGA CACCCTTGGG CACCAAGGAG 
AACCCAGCCC GGGTCTGCCG GGACCTCATG GACTGTGAGC AGAGGATGGC GGATGGTACC 
TACTGGGTGG ACCCCAACCT CGGCTGCTCC TCTGACACCA TTGAAGTCTC CTGCAACTTT 
ACACAGGGTG GGCAGACGTG CCTGAAGCCC ATCACGGCCT CCAAGGCCGA GTTTGCTGTG 
AGTCGGGTCC AGATGAATTT CTTGCACCTG CTGAGCTCTG AGGGGACACA GCATATCACA 
ATCCACTGTC TGAACATGAC GGTGTGGCAG GAGGGACCGG GACGCTCCTC TGCCAGACAG 
GCTGTGCGCT TCCGTGCCTG GAACGGACAG GTCTTCGAAG CTGGGGGTCA GTTCAGGCCA 
GAGGTGTCTA TGGATGGCTG CAAGGTCCAT GATGGCCGCT GGCATCAGAC ACTGTTCACC 
TTCCGGACCC AGGACCCCCA GCAGCTGCCC ATCGTCAGTG TGGACAATCT CCCGCCTGTC 
TCATCAGGGA AGCAGTACCG CCTGGAAGTT GGACCTGCGT GCTTCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030109490.1
CDS	551..5581
Translation	MVTACGQHRVPVPLPSRRDSMLDPQGSFLLGKVNPRAVQFEGALCQFSIHPVAQVAHNYCAHLRERCRQVDTYSPQVGTLFPWDSGPAFALHPEPALLGLGNLTRTPATLGARPVSRALAVTLAPAMPTKPLRTVHPDVSEHSSSQTPLSPAKQSARKTPSPSSSASLANSTRVYRPAAAQPRQITTTSPTKRSPTKPSVSPLSVTPMKSPHATQKTGVPSFTKPVPPTQKPAPFTSYLAPSKASSPTVRPVQKTFMTPRPPVPSPQPLRPTTGLSKKFTNPTVAKSKSKTTSWASKPVLARSSVPKTLQQTVLSQSPVSYLGSQTLAPALPPLGVGNPRTMPPTRDSALTPAGSKKFTGRETSKKTRQKSSPRKPEPLSPGKSARDASPRDLTTKPSRPSTPALVLAPAYLLSSSPQPTSSSFPFFHLLGPTPFPMLMGPPGSKGDCGLPGPPGLPGLPGSPGARGPRGPPGPYGNPGPPGPPGAKGQKGDPGLSPGQAHDGAKGNMGLPGLSGNPGPLGRKGHKGHPGAAGHPGEQGQPGPEGSPGAKGYPGRQGFPGPVGDPGPKGSRGYIGLPGLFGLPGSDGERGLPGVPGKRGEMGRPGFPGDFGERGPPGLDGNPGEIGLPGPPGVLGLIGDTGALGPVGYPGPKGMKGLMGGVGEPGLKGDKGEQGVPGVSGDPGFQGDKGSHGLPGLPGGRGKPGPLGKAGDKGSLGFPGPPGPEGFPGDIGPPGDNGPEGMKGKPGARGLPGPPGQLGPEGDEGPMGPPGVPGLEGQPGRKGFPGRPGLDGSKGEPGDPGRPGPVGEQGLMGFIGLVGEPGIVGEKGDRGVMGPPGAPGPKGSMGHPGTPGGIGNPGEPGPWGPPGSRGLPGMRGAKGHRGPRGPDGPAGEQGSKGLKGRVGPRGRPGQPGQQGAAGERGHSGAKGFLGIPGPSGPPGAKGLPGEPGSQGPQGPVGPPGEMGPKGPPGAVGEPGLPGDSGMKGDLGPLGPPGEQGLIGQRGEPGLEGDHGPVGPDGLKGDRGDPGPDGEHGEKGQEGLKGEDGSPGPPGITGVPGREGKPGKQGEKGQRGAKGAKGHQGYLGEMGIPGEPGPPGTPGPKGSRGTLGPTGAPGRMGAQGEPGLAGYNGHKGITGPLGPPGPKGEKGDQGEDGKTEGPPGPPGDRGPVGDRGDRGEPGDPGYPGQEGVQGLRGEPGQQGQPGHPGPRGRPGPKGSKGEEGPKGKPGKAGPSGRRGTQGLQGLPGPRGVVGRQGPEGTAGSDGIPGRDGRPGYQGDQGNDGDPGPVGPAGRRGNPGVAGLPGAQGPPGFKGESGLPGQLGPPGKRGTEGGTGLPGNQGEPGSKGQPGDSGEMGFPGVAGLFGPKGPPGDIGFKGIQGPRGPPGLMGKEGIIGPPGMLGPSGLPGPKGDRGSRGDLGLQGPRGPPGPRGRPGPPGPPWHPIQFQQDDLGAAFQTWMDAQGAVRSEQGYSYPDQLALDQGGEIFKTLHYLSNLIQSIKTPLGTKENPARVCRDLMDCEQRMADGTYWVDPNLGCSSDTIEVSCNFTQGGQTCLKPITASKAEFAVSRVQMNFLHLLSSEGTQHITIHCLNMTVWQEGPGRSSARQAVRFRAWNGQVFEAGGQFRPEVSMDGCKVHDGRWHQTLFTFRTQDPQQLPIVSVDNLPPVSSGKQYRLEVGPACFL

Target ORF information:

RefSeq Version	XM_030253630.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030253630.1

ORF Insert Sequence:

ATGGTGACAG CCTGTGGGCA GCACAGGGTA CCTGTCCCAC TGCCTTCCCG TAGGGACTCC 
ATGCTTGACC CCCAGGGCTC CTTTCTCTTG GGGAAGGTGA ACCCCCGTGC TGTCCAGTTT 
GAAGGTGCAC TCTGTCAGTT CAGCATCCAC CCTGTGGCGC AGGTCGCTCA CAATTACTGT 
GCCCACCTGA GAGAGCGGTG CCGACAGGTG GACACATATA GTCCCCAGGT GGGAACCCTC 
TTTCCTTGGG ACTCTGGCCC AGCCTTTGCT TTGCATCCTG AACCAGCCTT GCTTGGCTTG 
GGGAATCTGA CCAGAACTCC AGCAACCCTG GGGGCCAGGC CTGTAAGCAG GGCACTTGCA 
GTGACTCTGG CTCCTGCCAT GCCCACCAAG CCCCTGAGGA CTGTCCACCC AGATGTCTCT 
GAACACAGCT CTTCCCAGAC CCCACTGTCA CCTGCCAAAC AGTCGGCCAG GAAAACACCT 
TCCCCCTCCT CGTCAGCTTC CCTGGCTAAT TCCACCAGGG TTTATCGTCC AGCTGCTGCC 
CAGCCAAGAC AGATCACAAC TACTAGCCCC ACCAAACGTT CCCCCACCAA GCCTTCTGTT 
TCTCCCCTTT CAGTTACCCC CATGAAAAGT CCCCATGCAA CCCAGAAAAC AGGTGTCCCT 
TCATTCACAA AGCCTGTTCC ACCCACTCAG AAGCCAGCGC CCTTCACCTC CTACCTAGCA 
CCTTCCAAAG CCTCTAGTCC CACTGTGAGG CCTGTTCAGA AGACTTTCAT GACACCCCGA 
CCTCCAGTCC CCAGCCCCCA GCCCCTGCGC CCTACTACTG GCTTATCTAA GAAGTTCACT 
AACCCCACTG TTGCAAAATC TAAGTCCAAG ACGACCAGTT GGGCCAGCAA GCCGGTCTTG 
GCCCGCTCCA GTGTCCCTAA AACGCTTCAG CAAACTGTTT TGTCCCAGTC TCCTGTTTCC 
TATCTGGGCT CTCAGACACT GGCTCCAGCC CTCCCTCCTT TGGGTGTTGG AAATCCTAGA 
ACGATGCCTC CCACACGTGA CTCTGCTTTA ACTCCTGCTG GAAGCAAGAA ATTCACAGGA 
CGGGAAACCT CCAAGAAAAC CAGACAGAAG AGCAGCCCCC GGAAGCCCGA ACCTCTCAGC 
CCTGGGAAGT CAGCCAGGGA TGCCTCACCA AGAGACCTGA CAACCAAGCC TAGCCGGCCG 
TCCACCCCAG CCCTGGTCCT TGCCCCAGCT TACCTCCTGT CCTCCAGCCC CCAGCCCACC 
AGCAGCAGCT TCCCTTTCTT CCACCTGCTG GGCCCTACAC CTTTCCCGAT GCTGATGGGG 
CCTCCAGGCT CCAAGGGAGA CTGTGGCTTG CCGGGTCCCC CTGGGCTTCC AGGATTACCT 
GGATCTCCGG GCGCACGAGG TCCTCGGGGT CCTCCTGGGC CGTACGGGAA CCCAGGCCCA 
CCTGGCCCTC CAGGAGCCAA AGGACAGAAA GGGGACCCAG GACTCTCACC AGGACAGGCT 
CACGATGGAG CAAAGGGCAA CATGGGCTTG CCTGGGCTCT CCGGAAATCC AGGACCCTTG 
GGAAGGAAGG GACACAAGGG CCATCCTGGA GCAGCAGGGC ACCCTGGAGA ACAGGGGCAG 
CCAGGACCTG AGGGCAGTCC AGGGGCCAAA GGTTACCCTG GCAGGCAGGG GTTCCCTGGA 
CCTGTAGGAG ACCCTGGCCC CAAAGGCAGC AGGGGTTACA TTGGTCTTCC GGGGCTCTTT 
GGCCTGCCAG GGTCGGATGG AGAGCGGGGT CTCCCTGGCG TTCCTGGCAA GAGGGGCGAG 
ATGGGTAGGC CGGGCTTCCC TGGAGACTTT GGGGAAAGAG GCCCCCCTGG ACTTGATGGC 
AACCCTGGAG AAATTGGCCT GCCGGGGCCA CCAGGAGTGC TCGGGCTCAT TGGTGACACA 
GGAGCACTGG GCCCAGTTGG CTACCCAGGA CCGAAAGGCA TGAAGGGACT GATGGGCGGC 
GTGGGGGAGC CCGGACTGAA AGGTGATAAG GGTGAACAAG GGGTGCCAGG TGTGTCGGGA 
GATCCTGGCT TCCAAGGAGA CAAGGGGAGT CATGGCTTGC CAGGGCTCCC GGGTGGCCGG 
GGGAAGCCAG GGCCTCTGGG AAAAGCTGGA GACAAAGGAT CACTTGGGTT TCCTGGACCT 
CCTGGACCTG AGGGTTTCCC AGGAGACATT GGCCCTCCAG GGGACAATGG CCCTGAAGGC 
ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG CCTGGACCTC CTGGGCAGCT GGGGCCTGAG 
GGAGATGAAG GACCCATGGG GCCACCAGGA GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG 
AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT GGCTCGAAGG GGGAACCAGG GGATCCTGGA 
CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT ATGGGATTCA TTGGTCTTGT CGGAGAGCCT 
GGAATTGTTG GGGAGAAGGG AGACCGTGGG GTGATGGGAC CCCCAGGTGC ACCTGGACCC 
AAGGGGTCGA TGGGCCATCC TGGAACACCC GGTGGTATTG GGAACCCTGG AGAGCCTGGA 
CCCTGGGGCC CTCCAGGATC TCGAGGCCTG CCAGGCATGA GGGGAGCCAA GGGGCACCGG 
GGCCCTCGAG GACCTGATGG ACCAGCTGGG GAGCAGGGAT CCAAGGGCCT GAAGGGTCGT 
GTAGGACCTC GGGGCAGACC TGGCCAGCCA GGACAGCAGG GTGCAGCTGG TGAGCGAGGC 
CACTCAGGTG CAAAAGGCTT CCTTGGCATC CCTGGTCCCT CAGGCCCTCC AGGTGCCAAG 
GGCCTCCCAG GAGAACCGGG CTCTCAGGGA CCTCAGGGGC CAGTTGGTCC TCCAGGAGAA 
ATGGGGCCCA AGGGACCGCC GGGTGCAGTG GGAGAGCCTG GCCTTCCTGG GGACTCCGGA 
ATGAAGGGTG ACCTCGGACC TCTGGGCCCC CCTGGGGAGC AAGGTCTTAT TGGGCAGCGG 
GGAGAGCCAG GCCTTGAGGG AGACCATGGC CCTGTGGGGC CAGATGGGCT GAAGGGCGAC 
AGGGGCGACC CAGGGCCCGA TGGCGAGCAT GGTGAAAAGG GCCAGGAAGG GCTGAAAGGA 
GAGGATGGGT CCCCTGGGCC CCCGGGCATC ACTGGCGTCC CGGGTCGTGA GGGGAAGCCG 
GGCAAGCAGG GTGAGAAAGG CCAGAGGGGA GCTAAGGGTG CCAAGGGCCA CCAAGGATAT 
CTGGGAGAGA TGGGCATCCC CGGAGAACCT GGGCCCCCTG GGACCCCAGG TCCTAAAGGG 
TCCCGGGGCA CCCTAGGACC AACGGGTGCT CCAGGACGGA TGGGAGCCCA AGGAGAACCA 
GGATTGGCCG GTTATAATGG ACACAAAGGC ATCACAGGAC CCCTCGGGCC TCCCGGGCCC 
AAAGGCGAGA AGGGGGACCA GGGCGAGGAT GGCAAGACCG AGGGGCCCCC TGGGCCACCA 
GGAGATCGGG GTCCTGTGGG TGATCGAGGA GACCGTGGGG AGCCAGGCGA CCCTGGATAC 
CCTGGTCAAG AGGGAGTTCA AGGCCTCCGT GGAGAACCGG GCCAGCAGGG ACAGCCTGGG 
CATCCAGGAC CCCGGGGGCG CCCAGGACCC AAAGGATCAA AAGGCGAAGA GGGCCCAAAG 
GGAAAGCCAG GCAAGGCTGG GCCATCAGGC CGGAGGGGGA CCCAGGGCCT TCAAGGACTG 
CCAGGCCCCC GAGGCGTGGT GGGGAGACAG GGCCCTGAGG GCACTGCTGG ATCTGATGGG 
ATTCCTGGCA GAGATGGTCG GCCAGGATAT CAGGGAGACC AGGGAAATGA TGGGGACCCT 
GGCCCTGTGG GCCCTGCTGG GAGAAGAGGA AATCCAGGTG TGGCTGGCTT GCCTGGAGCA 
CAGGGGCCTC CGGGATTCAA GGGTGAAAGT GGGTTACCTG GGCAACTGGG TCCCCCTGGA 
AAACGAGGGA CAGAAGGTGG AACAGGGCTT CCTGGGAACC AGGGGGAGCC AGGATCCAAA 
GGCCAGCCGG GTGACTCTGG CGAGATGGGC TTCCCAGGAG TGGCTGGCCT CTTTGGACCC 
AAGGGCCCCC CTGGAGACAT TGGCTTCAAA GGCATCCAAG GTCCTCGGGG GCCTCCTGGC 
TTGATGGGAA AGGAAGGTAT CATTGGCCCC CCCGGAATGC TGGGACCTTC TGGACTCCCG 
GGTCCCAAAG GTGACAGAGG CAGCCGAGGA GACTTGGGAC TGCAAGGCCC AAGGGGTCCT 
CCTGGTCCAA GGGGCCGGCC AGGTCCCCCG GGCCCTCCTT GGCATCCCAT CCAGTTTCAG 
CAAGATGACC TTGGAGCAGC TTTCCAGACA TGGATGGATG CTCAAGGAGC CGTCAGATCG 
GAGCAGGGGT ACAGCTATCC GGACCAGCTG GCGCTAGACC AGGGAGGGGA GATCTTCAAA 
ACCTTACACT ACCTCAGCAA CCTCATCCAG AGCATTAAGA CACCCTTGGG CACCAAGGAG 
AACCCAGCCC GGGTCTGCCG GGACCTCATG GACTGTGAGC AGAGGATGGC GGATGGTACC 
TACTGGGTGG ACCCCAACCT CGGCTGCTCC TCTGACACCA TTGAAGTCTC CTGCAACTTT 
ACACAGGGTG GGCAGACGTG CCTGAAGCCC ATCACGGCCT CCAAGGCCGA GTTTGCTGTG 
AGTCGGGTCC AGATGAATTT CTTGCACCTG CTGAGCTCTG AGGGGACACA GCATATCACA 
ATCCACTGTC TGAACATGAC GGTGTGGCAG GAGGGACCGG GACGCTCCTC TGCCAGACAG 
GCTGTGCGCT TCCGTGCCTG GAACGGACAG GTCTTCGAAG CTGGGGGTCA GTTCAGGCCA 
GAGGTGTCTA TGGATGGCTG CAAGGTCCAT GATGGCCGCT GGCATCAGAC ACTGTTCACC 
TTCCGGACCC AGGACCCCCA GCAGCTGCCC ATCGTCAGTG TGGACAATCT CCCGCCTGTC 
TCATCAGGGA AGCAGTACCG CCTGGAAGTT GGACCTGCGT GCTTCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col27a1 ( XM_030253631.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030109491.1 Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X4, mRNA.

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CloneID	OMu88566
Clone ID Related Accession (Same CDS sequence)	XM_030253631.1
Accession Version	XM_030253631.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4677bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	collagen alpha-1(XXVII) chain isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCACGG GATTCGCGCG GGGGGCCCGA GGCACAGCGG CGTCAGGACC CGGGGGGGGG 
TTTCTCTTCG CCTGGATCTT GGTCTCATTT ACCTGTCACC TGGCCTCCAC CCAAGGAGCT 
CCTGAAGATG TGGATGTCCT CCAGCGGCTG GGCCTCAGCT GGACGAAGGC CGGGGGTGGC 
CGGAGTCCTA CACCCCCTGG TGTCATTCCT TTCCCATCTG GCTTCATCTT CACACAGCGG 
GCCAAGCTGC AGGCCCCCAC AGCCAATGTT CTTCCCACCA CCCTGGGGCG GGAGCTGGCA 
TTGGTGTTGA GCCTCTGTTC ACATCGTGTG AACCATGCCT TCCTCTTTGC CATCCGCAGC 
AGGAAACACA AGCTGCAACT GGGTCTGCAG TTCCTCCCCG GCAGGACGAT CATCCACCTG 
GGGCCTCGGC AATCAGTGGC CTTCGACTTG GATGTGCATG ATGGGCGCTG GCACCACCTG 
GCCCTAGAGT TGCGTGGCCG CACAGTCACA ATGGTGACAG CCTGTGGGCA GCACAGGGTA 
CCTGTCCCAC TGCCTTCCCG TAGGGACTCC ATGCTTGACC CCCAGGGCTC CTTTCTCTTG 
GGGAAGGTGA ACCCCCGTGC TGTCCAGTTT GAAGGTGCAC TCTGTCAGTT CAGCATCCAC 
CCTGTGGCGC AGGTCGCTCA CAATTACTGT GCCCACCTGA GAGAGCGGTG CCGACAGGTG 
GACACATATA GTCCCCAGGT GGGAACCCTC TTTCCTTGGG ACTCTGGCCC AGCCTTTGCT 
TTGCATCCTG AACCAGCCTT GCTTGGCTTG GGGAATCTGA CCAGAACTCC AGCAACCCTG 
GGGGCCAGGC CTGTAAGCAG GGCACTTGCA GTGACTCTGG CTCCTGCCAT GCCCACCAAG 
CCCCTGAGGA CTGTCCACCC AGATGTCTCT GAACACAGCT CTTCCCAGAC CCCACTGTCA 
CCTGCCAAAC AGTCGGCCAG GAAAACACCT TCCCCCTCCT CGTCAGCTTC CCTGGCTAAT 
TCCACCAGGG TTTATCGTCC AGCTGCTGCC CAGCCAAGAC AGATCACAAC TACTAGCCCC 
ACCAAACGTT CCCCCACCAA GCCTTCTGTT TCTCCCCTTT CAGTTACCCC CATGAAAAGT 
CCCCATGCAA CCCAGAAAAC AGGTGTCCCT TCATTCACAA AGCCTGTTCC ACCCACTCAG 
AAGCCAGCGC CCTTCACCTC CTACCTAGCA CCTTCCAAAG CCTCTAGTCC CACTGTGAGG 
CCTGTTCAGA AGACTTTCAT GACACCCCGA CCTCCAGTCC CCAGCCCCCA GCCCCTGCGC 
CCTACTACTG GCTTATCTAA GAAGTTCACT AACCCCACTG TTGCAAAATC TAAGTCCAAG 
ACGACCAGTT GGGCCAGCAA GCCGGTCTTG GCCCGCTCCA GTGTCCCTAA AACGCTTCAG 
CAAACTGTTT TGTCCCAGTC TCCTGTTTCC TATCTGGGCT CTCAGACACT GGCTCCAGCC 
CTCCCTCCTT TGGGTGTTGG AAATCCTAGA ACGATGCCTC CCACACGTGA CTCTGCTTTA 
ACTCCTGCTG GAAGCAAGAA ATTCACAGGA CGGGAAACCT CCAAGAAAAC CAGACAGAAG 
AGCAGCCCCC GGAAGCCCGA ACCTCTCAGC CCTGGGAAGT CAGCCAGGGA TGCCTCACCA 
AGAGACCTGA CAACCAAGCC TAGCCGGCCG TCCACCCCAG CCCTGGTCCT TGCCCCAGCT 
TACCTCCTGT CCTCCAGCCC CCAGCCCACC AGCAGCAGCT TCCCTTTCTT CCACCTGCTG 
GGCCCTACAC CTTTCCCGAT GCTGATGGGG CCTCCAGGCT CCAAGGGAGA CTGTGGCTTG 
CCGGGTCCCC CTGGGCTTCC AGGATTACCT GGATCTCCGG GCGCACGAGG TCCTCGGGGT 
CCTCCTGGGC CGTACGGGAA CCCAGGCCCA CCTGGCCCTC CAGGAGCCAA AGGACAGAAA 
GGGGACCCAG GACTCTCACC AGGACAGGCT CACGATGGAG CAAAGGGCAA CATGGGCTTG 
CCTGGGCTCT CCGGAAATCC AGGACCCTTG GGAAGGAAGG GACACAAGGG CCATCCTGGA 
GCAGCAGGGC ACCCTGGAGA ACAGGGGCAG CCAGGACCTG AGGGCAGTCC AGGGGCCAAA 
GGTTACCCTG GCAGGCAGGG GTTCCCTGGA CCTGTAGGAG ACCCTGGCCC CAAAGGCAGC 
AGGGGTTACA TTGGTCTTCC GGGGCTCTTT GGCCTGCCAG GGTCGGATGG AGAGCGGGGT 
CTCCCTGGCG TTCCTGGCAA GAGGGGCGAG ATGGGTAGGC CGGGCTTCCC TGGAGACTTT 
GGGGAAAGAG GCCCCCCTGG ACTTGATGGC AACCCTGGAG AAATTGGCCT GCCGGGGCCA 
CCAGGAGTGC TCGGGCTCAT TGGTGACACA GGAGCACTGG GCCCAGTTGG CTACCCAGGA 
CCGAAAGGCA TGAAGGGACT GATGGGCGGC GTGGGGGAGC CCGGACTGAA AGGTGATAAG 
GGTGAACAAG GGGTGCCAGG TGTGTCGGGA GATCCTGGCT TCCAAGGAGA CAAGGGGAGT 
CATGGCTTGC CAGGGCTCCC GGGTGGCCGG GGGAAGCCAG GGCCTCTGGG AAAAGCTGGA 
GACAAAGGAT CACTTGGGTT TCCTGGACCT CCTGGACCTG AGGGTTTCCC AGGAGACATT 
GGCCCTCCAG GGGACAATGG CCCTGAAGGC ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG 
CCTGGACCTC CTGGGCAGCT GGGGCCTGAG GGAGATGAAG GACCCATGGG GCCACCAGGA 
GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT 
GGCTCGAAGG GGGAACCAGG GGATCCTGGA CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT 
ATGGGATTCA TTGGTCTTGT CGGAGAGCCT GGAATTGTTG GGGAGAAGGG AGACCGTGGG 
GTGATGGGAC CCCCAGGTGC ACCTGGACCC AAGGGGTCGA TGGGCCATCC TGGAACACCC 
GGTGGTATTG GGAACCCTGG AGAGCCTGGA CCCTGGGGCC CTCCAGGATC TCGAGGCCTG 
CCAGGCATGA GGGGAGCCAA GGGGCACCGG GGCCCTCGAG GACCTGATGG ACCAGCTGGG 
GAGCAGGGAT CCAAGGGCCT GAAGGGTCGT GTAGGACCTC GGGGCAGACC TGGCCAGCCA 
GGACAGCAGG GTGCAGCTGG TGAGCGAGGC CACTCAGGTG CAAAAGGCTT CCTTGGCATC 
CCTGGTCCCT CAGGCCCTCC AGGTGCCAAG GGCCTCCCAG GAGAACCGGG CTCTCAGGGA 
CCTCAGGGGC CAGTTGGTCC TCCAGGAGAA ATGGGGCCCA AGGGACCGCC GGGTGCAGTG 
GGAGAGCCTG GCCTTCCTGG GGACTCCGGA ATGAAGGGTG ACCTCGGACC TCTGGGCCCC 
CCTGGGGAGC AAGGTCTTAT TGGGCAGCGG GGAGAGCCAG GCCTTGAGGG AGACCATGGC 
CCTGTGGGGC CAGATGGGCT GAAGGGCGAC AGGGGCGACC CAGGGCCCGA TGGCGAGCAT 
GGTGAAAAGG GCCAGGAAGG GCTGAAAGGA GAGGATGGGT CCCCTGGGCC CCCGGGCATC 
ACTGGCGTCC CGGGTCGTGA GGGGAAGCCG GGCAAGCAGG GTGAGAAAGG CCAGAGGGGA 
GCTAAGGGTG CCAAGGGCCA CCAAGGATAT CTGGGAGAGA TGGGCATCCC CGGAGAACCT 
GGGCCCCCTG GGACCCCAGG TCCTAAAGGG TCCCGGGGCA CCCTAGGACC AACGGGTGCT 
CCAGGACGGA TGGGAGCCCA AGGAGAACCA GGATTGGCCG GTTATAATGG ACACAAAGGC 
ATCACAGGAC CCCTCGGGCC TCCCGGGCCC AAAGGCGAGA AGGGGGACCA GGGCGAGGAT 
GGCAAGACCG AGGGGCCCCC TGGGCCACCA GGAGATCGGG GTCCTGTGGG TGATCGAGGA 
GACCGTGGGG AGCCAGGCGA CCCTGGATAC CCTGGTCAAG AGGGAGTTCA AGGCCTCCGT 
GGAGAACCGG GCCAGCAGGG ACAGCCTGGG CATCCAGGAC CCCGGGGGCG CCCAGGACCC 
AAAGGATCAA AAGGCGAAGA GGGCCCAAAG GGAAAGCCAG GCAAGGCTGG GCCATCAGGC 
CGGAGGGGGA CCCAGGGCCT TCAAGGACTG CCAGGCCCCC GAGGCGTGGT GGGGAGACAG 
GGCCCTGAGG GCACTGCTGG ATCTGATGGG ATTCCTGGCA GAGATGGTCG GCCAGGATAT 
CAGGGAGACC AGGGAAATGA TGGGGACCCT GGCCCTGTGG GCCCTGCTGG GAGAAGAGGA 
AATCCAGGTG TGGCTGGCTT GCCTGGAGCA CAGGGGCCTC CGGGATTCAA GGGTGAAAGT 
GGGTTACCTG GGCAACTGGG TCCCCCTGGA AAACGAGGGA CAGAAGGTGG AACAGGGCTT 
CCTGGGAACC AGGGGGAGCC AGGATCCAAA GGCCAGCCGG GTGACTCTGG CGAGATGGGC 
TTCCCAGGAG TGGCTGGCCT CTTTGGACCC AAGGGCCCCC CTGGAGACAT TGGCTTCAAA 
GGCATCCAAG GTCCTCGGGG GCCTCCTGGC TTGATGGCCC AGATCGAGGA CTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030109491.1
CDS	509..5185
Translation	MGTGFARGARGTAASGPGGGFLFAWILVSFTCHLASTQGAPEDVDVLQRLGLSWTKAGGGRSPTPPGVIPFPSGFIFTQRAKLQAPTANVLPTTLGRELALVLSLCSHRVNHAFLFAIRSRKHKLQLGLQFLPGRTIIHLGPRQSVAFDLDVHDGRWHHLALELRGRTVTMVTACGQHRVPVPLPSRRDSMLDPQGSFLLGKVNPRAVQFEGALCQFSIHPVAQVAHNYCAHLRERCRQVDTYSPQVGTLFPWDSGPAFALHPEPALLGLGNLTRTPATLGARPVSRALAVTLAPAMPTKPLRTVHPDVSEHSSSQTPLSPAKQSARKTPSPSSSASLANSTRVYRPAAAQPRQITTTSPTKRSPTKPSVSPLSVTPMKSPHATQKTGVPSFTKPVPPTQKPAPFTSYLAPSKASSPTVRPVQKTFMTPRPPVPSPQPLRPTTGLSKKFTNPTVAKSKSKTTSWASKPVLARSSVPKTLQQTVLSQSPVSYLGSQTLAPALPPLGVGNPRTMPPTRDSALTPAGSKKFTGRETSKKTRQKSSPRKPEPLSPGKSARDASPRDLTTKPSRPSTPALVLAPAYLLSSSPQPTSSSFPFFHLLGPTPFPMLMGPPGSKGDCGLPGPPGLPGLPGSPGARGPRGPPGPYGNPGPPGPPGAKGQKGDPGLSPGQAHDGAKGNMGLPGLSGNPGPLGRKGHKGHPGAAGHPGEQGQPGPEGSPGAKGYPGRQGFPGPVGDPGPKGSRGYIGLPGLFGLPGSDGERGLPGVPGKRGEMGRPGFPGDFGERGPPGLDGNPGEIGLPGPPGVLGLIGDTGALGPVGYPGPKGMKGLMGGVGEPGLKGDKGEQGVPGVSGDPGFQGDKGSHGLPGLPGGRGKPGPLGKAGDKGSLGFPGPPGPEGFPGDIGPPGDNGPEGMKGKPGARGLPGPPGQLGPEGDEGPMGPPGVPGLEGQPGRKGFPGRPGLDGSKGEPGDPGRPGPVGEQGLMGFIGLVGEPGIVGEKGDRGVMGPPGAPGPKGSMGHPGTPGGIGNPGEPGPWGPPGSRGLPGMRGAKGHRGPRGPDGPAGEQGSKGLKGRVGPRGRPGQPGQQGAAGERGHSGAKGFLGIPGPSGPPGAKGLPGEPGSQGPQGPVGPPGEMGPKGPPGAVGEPGLPGDSGMKGDLGPLGPPGEQGLIGQRGEPGLEGDHGPVGPDGLKGDRGDPGPDGEHGEKGQEGLKGEDGSPGPPGITGVPGREGKPGKQGEKGQRGAKGAKGHQGYLGEMGIPGEPGPPGTPGPKGSRGTLGPTGAPGRMGAQGEPGLAGYNGHKGITGPLGPPGPKGEKGDQGEDGKTEGPPGPPGDRGPVGDRGDRGEPGDPGYPGQEGVQGLRGEPGQQGQPGHPGPRGRPGPKGSKGEEGPKGKPGKAGPSGRRGTQGLQGLPGPRGVVGRQGPEGTAGSDGIPGRDGRPGYQGDQGNDGDPGPVGPAGRRGNPGVAGLPGAQGPPGFKGESGLPGQLGPPGKRGTEGGTGLPGNQGEPGSKGQPGDSGEMGFPGVAGLFGPKGPPGDIGFKGIQGPRGPPGLMAQIEDS

Target ORF information:

RefSeq Version	XM_030253631.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030253631.1

ORF Insert Sequence:

ATGGGCACGG GATTCGCGCG GGGGGCCCGA GGCACAGCGG CGTCAGGACC CGGGGGGGGG 
TTTCTCTTCG CCTGGATCTT GGTCTCATTT ACCTGTCACC TGGCCTCCAC CCAAGGAGCT 
CCTGAAGATG TGGATGTCCT CCAGCGGCTG GGCCTCAGCT GGACGAAGGC CGGGGGTGGC 
CGGAGTCCTA CACCCCCTGG TGTCATTCCT TTCCCATCTG GCTTCATCTT CACACAGCGG 
GCCAAGCTGC AGGCCCCCAC AGCCAATGTT CTTCCCACCA CCCTGGGGCG GGAGCTGGCA 
TTGGTGTTGA GCCTCTGTTC ACATCGTGTG AACCATGCCT TCCTCTTTGC CATCCGCAGC 
AGGAAACACA AGCTGCAACT GGGTCTGCAG TTCCTCCCCG GCAGGACGAT CATCCACCTG 
GGGCCTCGGC AATCAGTGGC CTTCGACTTG GATGTGCATG ATGGGCGCTG GCACCACCTG 
GCCCTAGAGT TGCGTGGCCG CACAGTCACA ATGGTGACAG CCTGTGGGCA GCACAGGGTA 
CCTGTCCCAC TGCCTTCCCG TAGGGACTCC ATGCTTGACC CCCAGGGCTC CTTTCTCTTG 
GGGAAGGTGA ACCCCCGTGC TGTCCAGTTT GAAGGTGCAC TCTGTCAGTT CAGCATCCAC 
CCTGTGGCGC AGGTCGCTCA CAATTACTGT GCCCACCTGA GAGAGCGGTG CCGACAGGTG 
GACACATATA GTCCCCAGGT GGGAACCCTC TTTCCTTGGG ACTCTGGCCC AGCCTTTGCT 
TTGCATCCTG AACCAGCCTT GCTTGGCTTG GGGAATCTGA CCAGAACTCC AGCAACCCTG 
GGGGCCAGGC CTGTAAGCAG GGCACTTGCA GTGACTCTGG CTCCTGCCAT GCCCACCAAG 
CCCCTGAGGA CTGTCCACCC AGATGTCTCT GAACACAGCT CTTCCCAGAC CCCACTGTCA 
CCTGCCAAAC AGTCGGCCAG GAAAACACCT TCCCCCTCCT CGTCAGCTTC CCTGGCTAAT 
TCCACCAGGG TTTATCGTCC AGCTGCTGCC CAGCCAAGAC AGATCACAAC TACTAGCCCC 
ACCAAACGTT CCCCCACCAA GCCTTCTGTT TCTCCCCTTT CAGTTACCCC CATGAAAAGT 
CCCCATGCAA CCCAGAAAAC AGGTGTCCCT TCATTCACAA AGCCTGTTCC ACCCACTCAG 
AAGCCAGCGC CCTTCACCTC CTACCTAGCA CCTTCCAAAG CCTCTAGTCC CACTGTGAGG 
CCTGTTCAGA AGACTTTCAT GACACCCCGA CCTCCAGTCC CCAGCCCCCA GCCCCTGCGC 
CCTACTACTG GCTTATCTAA GAAGTTCACT AACCCCACTG TTGCAAAATC TAAGTCCAAG 
ACGACCAGTT GGGCCAGCAA GCCGGTCTTG GCCCGCTCCA GTGTCCCTAA AACGCTTCAG 
CAAACTGTTT TGTCCCAGTC TCCTGTTTCC TATCTGGGCT CTCAGACACT GGCTCCAGCC 
CTCCCTCCTT TGGGTGTTGG AAATCCTAGA ACGATGCCTC CCACACGTGA CTCTGCTTTA 
ACTCCTGCTG GAAGCAAGAA ATTCACAGGA CGGGAAACCT CCAAGAAAAC CAGACAGAAG 
AGCAGCCCCC GGAAGCCCGA ACCTCTCAGC CCTGGGAAGT CAGCCAGGGA TGCCTCACCA 
AGAGACCTGA CAACCAAGCC TAGCCGGCCG TCCACCCCAG CCCTGGTCCT TGCCCCAGCT 
TACCTCCTGT CCTCCAGCCC CCAGCCCACC AGCAGCAGCT TCCCTTTCTT CCACCTGCTG 
GGCCCTACAC CTTTCCCGAT GCTGATGGGG CCTCCAGGCT CCAAGGGAGA CTGTGGCTTG 
CCGGGTCCCC CTGGGCTTCC AGGATTACCT GGATCTCCGG GCGCACGAGG TCCTCGGGGT 
CCTCCTGGGC CGTACGGGAA CCCAGGCCCA CCTGGCCCTC CAGGAGCCAA AGGACAGAAA 
GGGGACCCAG GACTCTCACC AGGACAGGCT CACGATGGAG CAAAGGGCAA CATGGGCTTG 
CCTGGGCTCT CCGGAAATCC AGGACCCTTG GGAAGGAAGG GACACAAGGG CCATCCTGGA 
GCAGCAGGGC ACCCTGGAGA ACAGGGGCAG CCAGGACCTG AGGGCAGTCC AGGGGCCAAA 
GGTTACCCTG GCAGGCAGGG GTTCCCTGGA CCTGTAGGAG ACCCTGGCCC CAAAGGCAGC 
AGGGGTTACA TTGGTCTTCC GGGGCTCTTT GGCCTGCCAG GGTCGGATGG AGAGCGGGGT 
CTCCCTGGCG TTCCTGGCAA GAGGGGCGAG ATGGGTAGGC CGGGCTTCCC TGGAGACTTT 
GGGGAAAGAG GCCCCCCTGG ACTTGATGGC AACCCTGGAG AAATTGGCCT GCCGGGGCCA 
CCAGGAGTGC TCGGGCTCAT TGGTGACACA GGAGCACTGG GCCCAGTTGG CTACCCAGGA 
CCGAAAGGCA TGAAGGGACT GATGGGCGGC GTGGGGGAGC CCGGACTGAA AGGTGATAAG 
GGTGAACAAG GGGTGCCAGG TGTGTCGGGA GATCCTGGCT TCCAAGGAGA CAAGGGGAGT 
CATGGCTTGC CAGGGCTCCC GGGTGGCCGG GGGAAGCCAG GGCCTCTGGG AAAAGCTGGA 
GACAAAGGAT CACTTGGGTT TCCTGGACCT CCTGGACCTG AGGGTTTCCC AGGAGACATT 
GGCCCTCCAG GGGACAATGG CCCTGAAGGC ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG 
CCTGGACCTC CTGGGCAGCT GGGGCCTGAG GGAGATGAAG GACCCATGGG GCCACCAGGA 
GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT 
GGCTCGAAGG GGGAACCAGG GGATCCTGGA CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT 
ATGGGATTCA TTGGTCTTGT CGGAGAGCCT GGAATTGTTG GGGAGAAGGG AGACCGTGGG 
GTGATGGGAC CCCCAGGTGC ACCTGGACCC AAGGGGTCGA TGGGCCATCC TGGAACACCC 
GGTGGTATTG GGAACCCTGG AGAGCCTGGA CCCTGGGGCC CTCCAGGATC TCGAGGCCTG 
CCAGGCATGA GGGGAGCCAA GGGGCACCGG GGCCCTCGAG GACCTGATGG ACCAGCTGGG 
GAGCAGGGAT CCAAGGGCCT GAAGGGTCGT GTAGGACCTC GGGGCAGACC TGGCCAGCCA 
GGACAGCAGG GTGCAGCTGG TGAGCGAGGC CACTCAGGTG CAAAAGGCTT CCTTGGCATC 
CCTGGTCCCT CAGGCCCTCC AGGTGCCAAG GGCCTCCCAG GAGAACCGGG CTCTCAGGGA 
CCTCAGGGGC CAGTTGGTCC TCCAGGAGAA ATGGGGCCCA AGGGACCGCC GGGTGCAGTG 
GGAGAGCCTG GCCTTCCTGG GGACTCCGGA ATGAAGGGTG ACCTCGGACC TCTGGGCCCC 
CCTGGGGAGC AAGGTCTTAT TGGGCAGCGG GGAGAGCCAG GCCTTGAGGG AGACCATGGC 
CCTGTGGGGC CAGATGGGCT GAAGGGCGAC AGGGGCGACC CAGGGCCCGA TGGCGAGCAT 
GGTGAAAAGG GCCAGGAAGG GCTGAAAGGA GAGGATGGGT CCCCTGGGCC CCCGGGCATC 
ACTGGCGTCC CGGGTCGTGA GGGGAAGCCG GGCAAGCAGG GTGAGAAAGG CCAGAGGGGA 
GCTAAGGGTG CCAAGGGCCA CCAAGGATAT CTGGGAGAGA TGGGCATCCC CGGAGAACCT 
GGGCCCCCTG GGACCCCAGG TCCTAAAGGG TCCCGGGGCA CCCTAGGACC AACGGGTGCT 
CCAGGACGGA TGGGAGCCCA AGGAGAACCA GGATTGGCCG GTTATAATGG ACACAAAGGC 
ATCACAGGAC CCCTCGGGCC TCCCGGGCCC AAAGGCGAGA AGGGGGACCA GGGCGAGGAT 
GGCAAGACCG AGGGGCCCCC TGGGCCACCA GGAGATCGGG GTCCTGTGGG TGATCGAGGA 
GACCGTGGGG AGCCAGGCGA CCCTGGATAC CCTGGTCAAG AGGGAGTTCA AGGCCTCCGT 
GGAGAACCGG GCCAGCAGGG ACAGCCTGGG CATCCAGGAC CCCGGGGGCG CCCAGGACCC 
AAAGGATCAA AAGGCGAAGA GGGCCCAAAG GGAAAGCCAG GCAAGGCTGG GCCATCAGGC 
CGGAGGGGGA CCCAGGGCCT TCAAGGACTG CCAGGCCCCC GAGGCGTGGT GGGGAGACAG 
GGCCCTGAGG GCACTGCTGG ATCTGATGGG ATTCCTGGCA GAGATGGTCG GCCAGGATAT 
CAGGGAGACC AGGGAAATGA TGGGGACCCT GGCCCTGTGG GCCCTGCTGG GAGAAGAGGA 
AATCCAGGTG TGGCTGGCTT GCCTGGAGCA CAGGGGCCTC CGGGATTCAA GGGTGAAAGT 
GGGTTACCTG GGCAACTGGG TCCCCCTGGA AAACGAGGGA CAGAAGGTGG AACAGGGCTT 
CCTGGGAACC AGGGGGAGCC AGGATCCAAA GGCCAGCCGG GTGACTCTGG CGAGATGGGC 
TTCCCAGGAG TGGCTGGCCT CTTTGGACCC AAGGGCCCCC CTGGAGACAT TGGCTTCAAA 
GGCATCCAAG GTCCTCGGGG GCCTCCTGGC TTGATGGCCC AGATCGAGGA CTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col27a1 ( NM_025685.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_079961.3 Mus musculus collagen, type XXVII, alpha 1 (Col27a1), mRNA.

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CloneID	OMu20706
Clone ID Related Accession (Same CDS sequence)	NM_025685.3
Accession Version	NM_025685.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5538bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	collagen alpha-1(XXVII) chain preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK157951.1, AL691496.13, AY167568.2 and AL683828.8. On Oct 12, 2006 this sequence version replaced NM_025685.2. ##Evidence-Data-START## Transcript exon combination :: AY167568.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849382 [ECO:0000348] ##Evidence-Data-END##

ATGGGCACGG GATTCGCGCG GGGGGCCCGA GGCACAGCGG CGTCAGGACC CGGGGGGGGG 
TTTCTCTTCG CCTGGATCTT GGTCTCATTT ACCTGTCACC TGGCCTCCAC CCAAGGAGCT 
CCTGAAGATG TGGATGTCCT CCAGCGGCTG GGCCTCAGCT GGACGAAGGC CGGGGGTGGC 
CGGAGTCCTA CACCCCCTGG TGTCATTCCT TTCCCATCTG GCTTCATCTT CACACAGCGG 
GCCAAGCTGC AGGCCCCCAC AGCCAATGTT CTTCCCACCA CCCTGGGGCG GGAGCTGGCA 
TTGGTGTTGA GCCTCTGTTC ACATCGTGTG AACCATGCCT TCCTCTTTGC CATCCGCAGC 
AGGAAACACA AGCTGCAACT GGGTCTGCAG TTCCTCCCCG GCAGGACGAT CATCCACCTG 
GGGCCTCGGC AATCAGTGGC CTTCGACTTG GATGTGCATG ATGGGCGCTG GCACCACCTG 
GCCCTAGAGT TGCGTGGCCG CACAGTCACA ATGGTGACAG CCTGTGGGCA GCACAGGGTA 
CCTGTCCCAC TGCCTTCCCG TAGGGACTCC ATGCTTGACC CCCAGGGCTC CTTTCTCTTG 
GGGAAGGTGA ACCCCCGTGC TGTCCAGTTT GAAGGTGCAC TCTGTCAGTT CAGCATCCAC 
CCTGTGGCGC AGGTCGCTCA CAATTACTGT GCCCACCTGA GAGAGCGGTG CCGACAGGTG 
GACACATATA GTCCCCAGGT GGGAACCCTC TTTCCTTGGG ACTCTGGCCC AGCCTTTGCT 
TTGCATCCTG AACCAGCCTT GCTTGGCTTG GGGAATCTGA CCAGAACTCC AGCAACCCTG 
GGGGCCAGGC CTGTAAGCAG GGCACTTGCA GTGACTCTGG CTCCTGCCAT GCCCACCAAG 
CCCCTGAGGA CTGTCCACCC AGATGTCTCT GAACACAGCT CTTCCCAGAC CCCACTGTCA 
CCTGCCAAAC AGTCGGCCAG GAAAACACCT TCCCCCTCCT CGTCAGCTTC CCTGGCTAAT 
TCCACCAGGG TTTATCGTCC AGCTGCTGCC CAGCCAAGAC AGATCACAAC TACTAGCCCC 
ACCAAACGTT CCCCCACCAA GCCTTCTGTT TCTCCCCTTT CAGTTACCCC CATGAAAAGT 
CCCCATGCAA CCCAGAAAAC AGGTGTCCCT TCATTCACAA AGCCTGTTCC ACCCACTCAG 
AAGCCAGCGC CCTTCACCTC CTACCTAGCA CCTTCCAAAG CCTCTAGTCC CACTGTGAGG 
CCTGTTCAGA AGACTTTCAT GACACCCCGA CCTCCAGTCC CCAGCCCCCA GCCCCTGCGC 
CCTACTACTG GCTTATCTAA GAAGTTCACT AACCCCACTG TTGCAAAATC TAAGTCCAAG 
ACGACCAGTT GGGCCAGCAA GCCGGTCTTG GCCCGCTCCA GTGTCCCTAA AACGCTTCAG 
CAAACTGTTT TGTCCCAGTC TCCTGTTTCC TATCTGGGCT CTCAGACACT GGCTCCAGCC 
CTCCCTCCTT TGGGTGTTGG AAATCCTAGA ACGATGCCTC CCACACGTGA CTCTGCTTTA 
ACTCCTGCTG GAAGCAAGAA ATTCACAGGA CGGGAAACCT CCAAGAAAAC CAGACAGAAG 
AGCAGCCCCC GGAAGCCCGA ACCTCTCAGC CCTGGGAAGT CAGCCAGGGA TGCCTCACCA 
AGAGACCTGA CAACCAAGCC TAGCCGGCCG TCCACCCCAG CCCTGGTCCT TGCCCCAGCT 
TACCTCCTGT CCTCCAGCCC CCAGCCCACC AGCAGCAGCT TCCCTTTCTT CCACCTGCTG 
GGCCCTACAC CTTTCCCGAT GCTGATGGGG CCTCCAGGCT CCAAGGGAGA CTGTGGCTTG 
CCGGGTCCCC CTGGGCTTCC AGGATTACCT GGATCTCCGG GCGCACGAGG TCCTCGGGGT 
CCTCCTGGGC CGTACGGGAA CCCAGGCCCA CCTGGCCCTC CAGGAGCCAA AGGACAGAAA 
GGGGACCCAG GACTCTCACC AGGACAGGCT CACGATGGAG CAAAGGGCAA CATGGGCTTG 
CCTGGGCTCT CCGGAAATCC AGGACCCTTG GGAAGGAAGG GACACAAGGG CCATCCTGGA 
GCAGCAGGGC ACCCTGGAGA ACAGGGGCAG CCAGGACCTG AGGGCAGTCC AGGGGCCAAA 
GGTTACCCTG GCAGGCAGGG GTTCCCTGGA CCTGTAGGAG ACCCTGGCCC CAAAGGCAGC 
AGGGGTTACA TTGGTCTTCC GGGGCTCTTT GGCCTGCCAG GGTCGGATGG AGAGCGGGGT 
CTCCCTGGCG TTCCTGGCAA GAGGGGCGAG ATGGGTAGGC CGGGCTTCCC TGGAGACTTT 
GGGGAAAGAG GCCCCCCTGG ACTTGATGGC AACCCTGGAG AAATTGGCCT GCCGGGGCCA 
CCAGGAGTGC TCGGGCTCAT TGGTGACACA GGAGCACTGG GCCCAGTTGG CTACCCAGGA 
CCGAAAGGCA TGAAGGGACT GATGGGCGGC GTGGGGGAGC CCGGACTGAA AGGTGATAAG 
GGTGAACAAG GGGTGCCAGG TGTGTCGGGA GATCCTGGCT TCCAAGGAGA CAAGGGGAGT 
CATGGCTTGC CAGGGCTCCC GGGTGGCCGG GGGAAGCCAG GGCCTCTGGG AAAAGCTGGA 
GACAAAGGAT CACTTGGGTT TCCTGGACCT CCTGGACCTG AGGGTTTCCC AGGAGACATT 
GGCCCTCCAG GGGACAATGG CCCTGAAGGC ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG 
CCTGGACCTC CTGGGCAGCT GGGGCCTGAG GGAGATGAAG GACCCATGGG GCCACCAGGA 
GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT 
GGCTCGAAGG GGGAACCAGG GGATCCTGGA CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT 
ATGGGATTCA TTGGTCTTGT CGGAGAGCCT GGAATTGTTG GGGAGAAGGG AGACCGTGGG 
GTGATGGGAC CCCCAGGTGC ACCTGGACCC AAGGGGTCGA TGGGCCATCC TGGAACACCC 
GGTGGTATTG GGAACCCTGG AGAGCCTGGA CCCTGGGGCC CTCCAGGATC TCGAGGCCTG 
CCAGGCATGA GGGGAGCCAA GGGGCACCGG GGCCCTCGAG GACCTGATGG ACCAGCTGGG 
GAGCAGGGAT CCAAGGGCCT GAAGGGTCGT GTAGGACCTC GGGGCAGACC TGGCCAGCCA 
GGACAGCAGG GTGCAGCTGG TGAGCGAGGC CACTCAGGTG CAAAAGGCTT CCTTGGCATC 
CCTGGTCCCT CAGGCCCTCC AGGTGCCAAG GGCCTCCCAG GAGAACCGGG CTCTCAGGGA 
CCTCAGGGGC CAGTTGGTCC TCCAGGAGAA ATGGGGCCCA AGGGACCGCC GGGTGCAGTG 
GGAGAGCCTG GCCTTCCTGG GGACTCCGGA ATGAAGGGTG ACCTCGGACC TCTGGGCCCC 
CCTGGGGAGC AAGGTCTTAT TGGGCAGCGG GGAGAGCCAG GCCTTGAGGG AGACCATGGC 
CCTGTGGGGC CAGATGGGCT GAAGGGCGAC AGGGGCGACC CAGGGCCCGA TGGCGAGCAT 
GGTGAAAAGG GCCAGGAAGG GCTGAAAGGA GAGGATGGGT CCCCTGGGCC CCCGGGCATC 
ACTGGCGTCC CGGGTCGTGA GGGGAAGCCG GGCAAGCAGG GTGAGAAAGG CCAGAGGGGA 
GCTAAGGGTG CCAAGGGCCA CCAAGGATAT CTGGGAGAGA TGGGCATCCC CGGAGAACCT 
GGGCCCCCTG GGACCCCAGG TCCTAAAGGG TCCCGGGGCA CCCTAGGACC AACGGGTGCT 
CCAGGACGGA TGGGAGCCCA AGGAGAACCA GGATTGGCCG GTTATAATGG ACACAAAGGC 
ATCACAGGAC CCCTCGGGCC TCCCGGGCCC AAAGGCGAGA AGGGGGACCA GGGCGAGGAT 
GGCAAGACCG AGGGGCCCCC TGGGCCACCA GGAGATCGGG GTCCTGTGGG TGATCGAGGA 
GACCGTGGGG AGCCAGGCGA CCCTGGATAC CCTGGTCAAG AGGGAGTTCA AGGCCTCCGT 
GGAGAACCGG GCCAGCAGGG ACAGCCTGGG CATCCAGGAC CCCGGGGGCG CCCAGGACCC 
AAAGGATCAA AAGGCGAAGA GGGCCCAAAG GGAAAGCCAG GCAAGGCTGG GCCATCAGGC 
CGGAGGGGGA CCCAGGGCCT TCAAGGACTG CCAGGCCCCC GAGGCGTGGT GGGGAGACAG 
GGCCCTGAGG GCACTGCTGG ATCTGATGGG ATTCCTGGCA GAGATGGTCG GCCAGGATAT 
CAGGGAGACC AGGGAAATGA TGGGGACCCT GGCCCTGTGG GCCCTGCTGG GAGAAGAGGA 
AATCCAGGTG TGGCTGGCTT GCCTGGAGCA CAGGGGCCTC CGGGATTCAA GGGTGAAAGT 
GGGTTACCTG GGCAACTGGG TCCCCCTGGA AAACGAGGGA CAGAAGGTGG AACAGGGCTT 
CCTGGGAACC AGGGGGAGCC AGGATCCAAA GGCCAGCCGG GTGACTCTGG CGAGATGGGC 
TTCCCAGGAG TGGCTGGCCT CTTTGGACCC AAGGGCCCCC CTGGAGACAT TGGCTTCAAA 
GGCATCCAAG GTCCTCGGGG GCCTCCTGGC TTGATGGGAA AGGAAGGTAT CATTGGCCCC 
CCCGGAATGC TGGGACCTTC TGGACTCCCG GGTCCCAAAG GTGACAGAGG CAGCCGAGGA 
GACTTGGGAC TGCAAGGCCC AAGGGGTCCT CCTGGTCCAA GGGGCCGGCC AGGTCCCCCG 
GGCCCTCCTT GGCATCCCAT CCAGTTTCAG CAAGATGACC TTGGAGCAGC TTTCCAGACA 
TGGATGGATG CTCAAGGAGC CGTCAGATCG GAGGGGTACA GCTATCCGGA CCAGCTGGCG 
CTAGACCAGG GAGGGGAGAT CTTCAAAACC TTACACTACC TCAGCAACCT CATCCAGAGC 
ATTAAGACAC CCTTGGGCAC CAAGGAGAAC CCAGCCCGGG TCTGCCGGGA CCTCATGGAC 
TGTGAGCAGA GGATGGCGGA TGGTACCTAC TGGGTGGACC CCAACCTCGG CTGCTCCTCT 
GACACCATTG AAGTCTCCTG CAACTTTACA CAGGGTGGGC AGACGTGCCT GAAGCCCATC 
ACGGCCTCCA AGGCCGAGTT TGCTGTGAGT CGGGTCCAGA TGAATTTCTT GCACCTGCTG 
AGCTCTGAGG GGACACAGCA TATCACAATC CACTGTCTGA ACATGACGGT GTGGCAGGAG 
GGACCGGGAC GCTCCTCTGC CAGACAGGCT GTGCGCTTCC GTGCCTGGAA CGGACAGGTC 
TTCGAAGCTG GGGGTCAGTT CAGGCCAGAG GTGTCTATGG ATGGCTGCAA GGTCCATGAT 
GGCCGCTGGC ATCAGACACT GTTCACCTTC CGGACCCAGG ACCCCCAGCA GCTGCCCATC 
GTCAGTGTGG ACAATCTCCC GCCTGTCTCA TCAGGGAAGC AGTACCGCCT GGAAGTTGGA 
CCTGCGTGCT TCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_079961.3
CDS	415..5952
Translation	MGTGFARGARGTAASGPGGGFLFAWILVSFTCHLASTQGAPEDVDVLQRLGLSWTKAGGGRSPTPPGVIPFPSGFIFTQRAKLQAPTANVLPTTLGRELALVLSLCSHRVNHAFLFAIRSRKHKLQLGLQFLPGRTIIHLGPRQSVAFDLDVHDGRWHHLALELRGRTVTMVTACGQHRVPVPLPSRRDSMLDPQGSFLLGKVNPRAVQFEGALCQFSIHPVAQVAHNYCAHLRERCRQVDTYSPQVGTLFPWDSGPAFALHPEPALLGLGNLTRTPATLGARPVSRALAVTLAPAMPTKPLRTVHPDVSEHSSSQTPLSPAKQSARKTPSPSSSASLANSTRVYRPAAAQPRQITTTSPTKRSPTKPSVSPLSVTPMKSPHATQKTGVPSFTKPVPPTQKPAPFTSYLAPSKASSPTVRPVQKTFMTPRPPVPSPQPLRPTTGLSKKFTNPTVAKSKSKTTSWASKPVLARSSVPKTLQQTVLSQSPVSYLGSQTLAPALPPLGVGNPRTMPPTRDSALTPAGSKKFTGRETSKKTRQKSSPRKPEPLSPGKSARDASPRDLTTKPSRPSTPALVLAPAYLLSSSPQPTSSSFPFFHLLGPTPFPMLMGPPGSKGDCGLPGPPGLPGLPGSPGARGPRGPPGPYGNPGPPGPPGAKGQKGDPGLSPGQAHDGAKGNMGLPGLSGNPGPLGRKGHKGHPGAAGHPGEQGQPGPEGSPGAKGYPGRQGFPGPVGDPGPKGSRGYIGLPGLFGLPGSDGERGLPGVPGKRGEMGRPGFPGDFGERGPPGLDGNPGEIGLPGPPGVLGLIGDTGALGPVGYPGPKGMKGLMGGVGEPGLKGDKGEQGVPGVSGDPGFQGDKGSHGLPGLPGGRGKPGPLGKAGDKGSLGFPGPPGPEGFPGDIGPPGDNGPEGMKGKPGARGLPGPPGQLGPEGDEGPMGPPGVPGLEGQPGRKGFPGRPGLDGSKGEPGDPGRPGPVGEQGLMGFIGLVGEPGIVGEKGDRGVMGPPGAPGPKGSMGHPGTPGGIGNPGEPGPWGPPGSRGLPGMRGAKGHRGPRGPDGPAGEQGSKGLKGRVGPRGRPGQPGQQGAAGERGHSGAKGFLGIPGPSGPPGAKGLPGEPGSQGPQGPVGPPGEMGPKGPPGAVGEPGLPGDSGMKGDLGPLGPPGEQGLIGQRGEPGLEGDHGPVGPDGLKGDRGDPGPDGEHGEKGQEGLKGEDGSPGPPGITGVPGREGKPGKQGEKGQRGAKGAKGHQGYLGEMGIPGEPGPPGTPGPKGSRGTLGPTGAPGRMGAQGEPGLAGYNGHKGITGPLGPPGPKGEKGDQGEDGKTEGPPGPPGDRGPVGDRGDRGEPGDPGYPGQEGVQGLRGEPGQQGQPGHPGPRGRPGPKGSKGEEGPKGKPGKAGPSGRRGTQGLQGLPGPRGVVGRQGPEGTAGSDGIPGRDGRPGYQGDQGNDGDPGPVGPAGRRGNPGVAGLPGAQGPPGFKGESGLPGQLGPPGKRGTEGGTGLPGNQGEPGSKGQPGDSGEMGFPGVAGLFGPKGPPGDIGFKGIQGPRGPPGLMGKEGIIGPPGMLGPSGLPGPKGDRGSRGDLGLQGPRGPPGPRGRPGPPGPPWHPIQFQQDDLGAAFQTWMDAQGAVRSEGYSYPDQLALDQGGEIFKTLHYLSNLIQSIKTPLGTKENPARVCRDLMDCEQRMADGTYWVDPNLGCSSDTIEVSCNFTQGGQTCLKPITASKAEFAVSRVQMNFLHLLSSEGTQHITIHCLNMTVWQEGPGRSSARQAVRFRAWNGQVFEAGGQFRPEVSMDGCKVHDGRWHQTLFTFRTQDPQQLPIVSVDNLPPVSSGKQYRLEVGPACFL

Target ORF information:

RefSeq Version	NM_025685.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type XXVII, alpha 1 (Col27a1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025685.3

ORF Insert Sequence:

ATGGGCACGG GATTCGCGCG GGGGGCCCGA GGCACAGCGG CGTCAGGACC CGGGGGGGGG 
TTTCTCTTCG CCTGGATCTT GGTCTCATTT ACCTGTCACC TGGCCTCCAC CCAAGGAGCT 
CCTGAAGATG TGGATGTCCT CCAGCGGCTG GGCCTCAGCT GGACGAAGGC CGGGGGTGGC 
CGGAGTCCTA CACCCCCTGG TGTCATTCCT TTCCCATCTG GCTTCATCTT CACACAGCGG 
GCCAAGCTGC AGGCCCCCAC AGCCAATGTT CTTCCCACCA CCCTGGGGCG GGAGCTGGCA 
TTGGTGTTGA GCCTCTGTTC ACATCGTGTG AACCATGCCT TCCTCTTTGC CATCCGCAGC 
AGGAAACACA AGCTGCAACT GGGTCTGCAG TTCCTCCCCG GCAGGACGAT CATCCACCTG 
GGGCCTCGGC AATCAGTGGC CTTCGACTTG GATGTGCATG ATGGGCGCTG GCACCACCTG 
GCCCTAGAGT TGCGTGGCCG CACAGTCACA ATGGTGACAG CCTGTGGGCA GCACAGGGTA 
CCTGTCCCAC TGCCTTCCCG TAGGGACTCC ATGCTTGACC CCCAGGGCTC CTTTCTCTTG 
GGGAAGGTGA ACCCCCGTGC TGTCCAGTTT GAAGGTGCAC TCTGTCAGTT CAGCATCCAC 
CCTGTGGCGC AGGTCGCTCA CAATTACTGT GCCCACCTGA GAGAGCGGTG CCGACAGGTG 
GACACATATA GTCCCCAGGT GGGAACCCTC TTTCCTTGGG ACTCTGGCCC AGCCTTTGCT 
TTGCATCCTG AACCAGCCTT GCTTGGCTTG GGGAATCTGA CCAGAACTCC AGCAACCCTG 
GGGGCCAGGC CTGTAAGCAG GGCACTTGCA GTGACTCTGG CTCCTGCCAT GCCCACCAAG 
CCCCTGAGGA CTGTCCACCC AGATGTCTCT GAACACAGCT CTTCCCAGAC CCCACTGTCA 
CCTGCCAAAC AGTCGGCCAG GAAAACACCT TCCCCCTCCT CGTCAGCTTC CCTGGCTAAT 
TCCACCAGGG TTTATCGTCC AGCTGCTGCC CAGCCAAGAC AGATCACAAC TACTAGCCCC 
ACCAAACGTT CCCCCACCAA GCCTTCTGTT TCTCCCCTTT CAGTTACCCC CATGAAAAGT 
CCCCATGCAA CCCAGAAAAC AGGTGTCCCT TCATTCACAA AGCCTGTTCC ACCCACTCAG 
AAGCCAGCGC CCTTCACCTC CTACCTAGCA CCTTCCAAAG CCTCTAGTCC CACTGTGAGG 
CCTGTTCAGA AGACTTTCAT GACACCCCGA CCTCCAGTCC CCAGCCCCCA GCCCCTGCGC 
CCTACTACTG GCTTATCTAA GAAGTTCACT AACCCCACTG TTGCAAAATC TAAGTCCAAG 
ACGACCAGTT GGGCCAGCAA GCCGGTCTTG GCCCGCTCCA GTGTCCCTAA AACGCTTCAG 
CAAACTGTTT TGTCCCAGTC TCCTGTTTCC TATCTGGGCT CTCAGACACT GGCTCCAGCC 
CTCCCTCCTT TGGGTGTTGG AAATCCTAGA ACGATGCCTC CCACACGTGA CTCTGCTTTA 
ACTCCTGCTG GAAGCAAGAA ATTCACAGGA CGGGAAACCT CCAAGAAAAC CAGACAGAAG 
AGCAGCCCCC GGAAGCCCGA ACCTCTCAGC CCTGGGAAGT CAGCCAGGGA TGCCTCACCA 
AGAGACCTGA CAACCAAGCC TAGCCGGCCG TCCACCCCAG CCCTGGTCCT TGCCCCAGCT 
TACCTCCTGT CCTCCAGCCC CCAGCCCACC AGCAGCAGCT TCCCTTTCTT CCACCTGCTG 
GGCCCTACAC CTTTCCCGAT GCTGATGGGG CCTCCAGGCT CCAAGGGAGA CTGTGGCTTG 
CCGGGTCCCC CTGGGCTTCC AGGATTACCT GGATCTCCGG GCGCACGAGG TCCTCGGGGT 
CCTCCTGGGC CGTACGGGAA CCCAGGCCCA CCTGGCCCTC CAGGAGCCAA AGGACAGAAA 
GGGGACCCAG GACTCTCACC AGGACAGGCT CACGATGGAG CAAAGGGCAA CATGGGCTTG 
CCTGGGCTCT CCGGAAATCC AGGACCCTTG GGAAGGAAGG GACACAAGGG CCATCCTGGA 
GCAGCAGGGC ACCCTGGAGA ACAGGGGCAG CCAGGACCTG AGGGCAGTCC AGGGGCCAAA 
GGTTACCCTG GCAGGCAGGG GTTCCCTGGA CCTGTAGGAG ACCCTGGCCC CAAAGGCAGC 
AGGGGTTACA TTGGTCTTCC GGGGCTCTTT GGCCTGCCAG GGTCGGATGG AGAGCGGGGT 
CTCCCTGGCG TTCCTGGCAA GAGGGGCGAG ATGGGTAGGC CGGGCTTCCC TGGAGACTTT 
GGGGAAAGAG GCCCCCCTGG ACTTGATGGC AACCCTGGAG AAATTGGCCT GCCGGGGCCA 
CCAGGAGTGC TCGGGCTCAT TGGTGACACA GGAGCACTGG GCCCAGTTGG CTACCCAGGA 
CCGAAAGGCA TGAAGGGACT GATGGGCGGC GTGGGGGAGC CCGGACTGAA AGGTGATAAG 
GGTGAACAAG GGGTGCCAGG TGTGTCGGGA GATCCTGGCT TCCAAGGAGA CAAGGGGAGT 
CATGGCTTGC CAGGGCTCCC GGGTGGCCGG GGGAAGCCAG GGCCTCTGGG AAAAGCTGGA 
GACAAAGGAT CACTTGGGTT TCCTGGACCT CCTGGACCTG AGGGTTTCCC AGGAGACATT 
GGCCCTCCAG GGGACAATGG CCCTGAAGGC ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG 
CCTGGACCTC CTGGGCAGCT GGGGCCTGAG GGAGATGAAG GACCCATGGG GCCACCAGGA 
GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT 
GGCTCGAAGG GGGAACCAGG GGATCCTGGA CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT 
ATGGGATTCA TTGGTCTTGT CGGAGAGCCT GGAATTGTTG GGGAGAAGGG AGACCGTGGG 
GTGATGGGAC CCCCAGGTGC ACCTGGACCC AAGGGGTCGA TGGGCCATCC TGGAACACCC 
GGTGGTATTG GGAACCCTGG AGAGCCTGGA CCCTGGGGCC CTCCAGGATC TCGAGGCCTG 
CCAGGCATGA GGGGAGCCAA GGGGCACCGG GGCCCTCGAG GACCTGATGG ACCAGCTGGG 
GAGCAGGGAT CCAAGGGCCT GAAGGGTCGT GTAGGACCTC GGGGCAGACC TGGCCAGCCA 
GGACAGCAGG GTGCAGCTGG TGAGCGAGGC CACTCAGGTG CAAAAGGCTT CCTTGGCATC 
CCTGGTCCCT CAGGCCCTCC AGGTGCCAAG GGCCTCCCAG GAGAACCGGG CTCTCAGGGA 
CCTCAGGGGC CAGTTGGTCC TCCAGGAGAA ATGGGGCCCA AGGGACCGCC GGGTGCAGTG 
GGAGAGCCTG GCCTTCCTGG GGACTCCGGA ATGAAGGGTG ACCTCGGACC TCTGGGCCCC 
CCTGGGGAGC AAGGTCTTAT TGGGCAGCGG GGAGAGCCAG GCCTTGAGGG AGACCATGGC 
CCTGTGGGGC CAGATGGGCT GAAGGGCGAC AGGGGCGACC CAGGGCCCGA TGGCGAGCAT 
GGTGAAAAGG GCCAGGAAGG GCTGAAAGGA GAGGATGGGT CCCCTGGGCC CCCGGGCATC 
ACTGGCGTCC CGGGTCGTGA GGGGAAGCCG GGCAAGCAGG GTGAGAAAGG CCAGAGGGGA 
GCTAAGGGTG CCAAGGGCCA CCAAGGATAT CTGGGAGAGA TGGGCATCCC CGGAGAACCT 
GGGCCCCCTG GGACCCCAGG TCCTAAAGGG TCCCGGGGCA CCCTAGGACC AACGGGTGCT 
CCAGGACGGA TGGGAGCCCA AGGAGAACCA GGATTGGCCG GTTATAATGG ACACAAAGGC 
ATCACAGGAC CCCTCGGGCC TCCCGGGCCC AAAGGCGAGA AGGGGGACCA GGGCGAGGAT 
GGCAAGACCG AGGGGCCCCC TGGGCCACCA GGAGATCGGG GTCCTGTGGG TGATCGAGGA 
GACCGTGGGG AGCCAGGCGA CCCTGGATAC CCTGGTCAAG AGGGAGTTCA AGGCCTCCGT 
GGAGAACCGG GCCAGCAGGG ACAGCCTGGG CATCCAGGAC CCCGGGGGCG CCCAGGACCC 
AAAGGATCAA AAGGCGAAGA GGGCCCAAAG GGAAAGCCAG GCAAGGCTGG GCCATCAGGC 
CGGAGGGGGA CCCAGGGCCT TCAAGGACTG CCAGGCCCCC GAGGCGTGGT GGGGAGACAG 
GGCCCTGAGG GCACTGCTGG ATCTGATGGG ATTCCTGGCA GAGATGGTCG GCCAGGATAT 
CAGGGAGACC AGGGAAATGA TGGGGACCCT GGCCCTGTGG GCCCTGCTGG GAGAAGAGGA 
AATCCAGGTG TGGCTGGCTT GCCTGGAGCA CAGGGGCCTC CGGGATTCAA GGGTGAAAGT 
GGGTTACCTG GGCAACTGGG TCCCCCTGGA AAACGAGGGA CAGAAGGTGG AACAGGGCTT 
CCTGGGAACC AGGGGGAGCC AGGATCCAAA GGCCAGCCGG GTGACTCTGG CGAGATGGGC 
TTCCCAGGAG TGGCTGGCCT CTTTGGACCC AAGGGCCCCC CTGGAGACAT TGGCTTCAAA 
GGCATCCAAG GTCCTCGGGG GCCTCCTGGC TTGATGGGAA AGGAAGGTAT CATTGGCCCC 
CCCGGAATGC TGGGACCTTC TGGACTCCCG GGTCCCAAAG GTGACAGAGG CAGCCGAGGA 
GACTTGGGAC TGCAAGGCCC AAGGGGTCCT CCTGGTCCAA GGGGCCGGCC AGGTCCCCCG 
GGCCCTCCTT GGCATCCCAT CCAGTTTCAG CAAGATGACC TTGGAGCAGC TTTCCAGACA 
TGGATGGATG CTCAAGGAGC CGTCAGATCG GAGGGGTACA GCTATCCGGA CCAGCTGGCG 
CTAGACCAGG GAGGGGAGAT CTTCAAAACC TTACACTACC TCAGCAACCT CATCCAGAGC 
ATTAAGACAC CCTTGGGCAC CAAGGAGAAC CCAGCCCGGG TCTGCCGGGA CCTCATGGAC 
TGTGAGCAGA GGATGGCGGA TGGTACCTAC TGGGTGGACC CCAACCTCGG CTGCTCCTCT 
GACACCATTG AAGTCTCCTG CAACTTTACA CAGGGTGGGC AGACGTGCCT GAAGCCCATC 
ACGGCCTCCA AGGCCGAGTT TGCTGTGAGT CGGGTCCAGA TGAATTTCTT GCACCTGCTG 
AGCTCTGAGG GGACACAGCA TATCACAATC CACTGTCTGA ACATGACGGT GTGGCAGGAG 
GGACCGGGAC GCTCCTCTGC CAGACAGGCT GTGCGCTTCC GTGCCTGGAA CGGACAGGTC 
TTCGAAGCTG GGGGTCAGTT CAGGCCAGAG GTGTCTATGG ATGGCTGCAA GGTCCATGAT 
GGCCGCTGGC ATCAGACACT GTTCACCTTC CGGACCCAGG ACCCCCAGCA GCTGCCCATC 
GTCAGTGTGG ACAATCTCCC GCCTGTCTCA TCAGGGAAGC AGTACCGCCT GGAAGTTGGA 
CCTGCGTGCT TCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col27a1 ( XM_006538035.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006538098.1 Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X4, mRNA.

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CloneID	OMu33005
Clone ID Related Accession (Same CDS sequence)	XM_006538035.4 , XM_006538035.3
Accession Version	XM_006538035.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5541bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	collagen alpha-1(XXVII) chain isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006538035.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCACGG GATTCGCGCG GGGGGCCCGA GGCACAGCGG CGTCAGGACC CGGGGGGGGG 
TTTCTCTTCG CCTGGATCTT GGTCTCATTT ACCTGTCACC TGGCCTCCAC CCAAGGAGCT 
CCTGAAGATG TGGATGTCCT CCAGCGGCTG GGCCTCAGCT GGACGAAGGC CGGGGGTGGC 
CGGAGTCCTA CACCCCCTGG TGTCATTCCT TTCCCATCTG GCTTCATCTT CACACAGCGG 
GCCAAGCTGC AGGCCCCCAC AGCCAATGTT CTTCCCACCA CCCTGGGGCG GGAGCTGGCA 
TTGGTGTTGA GCCTCTGTTC ACATCGTGTG AACCATGCCT TCCTCTTTGC CATCCGCAGC 
AGGAAACACA AGCTGCAACT GGGTCTGCAG TTCCTCCCCG GCAGGACGAT CATCCACCTG 
GGGCCTCGGC AATCAGTGGC CTTCGACTTG GATGTGCATG ATGGGCGCTG GCACCACCTG 
GCCCTAGAGT TGCGTGGCCG CACAGTCACA ATGGTGACAG CCTGTGGGCA GCACAGGGTA 
CCTGTCCCAC TGCCTTCCCG TAGGGACTCC ATGCTTGACC CCCAGGGCTC CTTTCTCTTG 
GGGAAGGTGA ACCCCCGTGC TGTCCAGTTT GAAGGTGCAC TCTGTCAGTT CAGCATCCAC 
CCTGTGGCGC AGGTCGCTCA CAATTACTGT GCCCACCTGA GAGAGCGGTG CCGACAGGTG 
GACACATATA GTCCCCAGGT GGGAACCCTC TTTCCTTGGG ACTCTGGCCC AGCCTTTGCT 
TTGCATCCTG AACCAGCCTT GCTTGGCTTG GGGAATCTGA CCAGAACTCC AGCAACCCTG 
GGGGCCAGGC CTGTAAGCAG GGCACTTGCA GTGACTCTGG CTCCTGCCAT GCCCACCAAG 
CCCCTGAGGA CTGTCCACCC AGATGTCTCT GAACACAGCT CTTCCCAGAC CCCACTGTCA 
CCTGCCAAAC AGTCGGCCAG GAAAACACCT TCCCCCTCCT CGTCAGCTTC CCTGGCTAAT 
TCCACCAGGG TTTATCGTCC AGCTGCTGCC CAGCCAAGAC AGATCACAAC TACTAGCCCC 
ACCAAACGTT CCCCCACCAA GCCTTCTGTT TCTCCCCTTT CAGTTACCCC CATGAAAAGT 
CCCCATGCAA CCCAGAAAAC AGGTGTCCCT TCATTCACAA AGCCTGTTCC ACCCACTCAG 
AAGCCAGCGC CCTTCACCTC CTACCTAGCA CCTTCCAAAG CCTCTAGTCC CACTGTGAGG 
CCTGTTCAGA AGACTTTCAT GACACCCCGA CCTCCAGTCC CCAGCCCCCA GCCCCTGCGC 
CCTACTACTG GCTTATCTAA GAAGTTCACT AACCCCACTG TTGCAAAATC TAAGTCCAAG 
ACGACCAGTT GGGCCAGCAA GCCGGTCTTG GCCCGCTCCA GTGTCCCTAA AACGCTTCAG 
CAAACTGTTT TGTCCCAGTC TCCTGTTTCC TATCTGGGCT CTCAGACACT GGCTCCAGCC 
CTCCCTCCTT TGGGTGTTGG AAATCCTAGA ACGATGCCTC CCACACGTGA CTCTGCTTTA 
ACTCCTGCTG GAAGCAAGAA ATTCACAGGA CGGGAAACCT CCAAGAAAAC CAGACAGAAG 
AGCAGCCCCC GGAAGCCCGA ACCTCTCAGC CCTGGGAAGT CAGCCAGGGA TGCCTCACCA 
AGAGACCTGA CAACCAAGCC TAGCCGGCCG TCCACCCCAG CCCTGGTCCT TGCCCCAGCT 
TACCTCCTGT CCTCCAGCCC CCAGCCCACC AGCAGCAGCT TCCCTTTCTT CCACCTGCTG 
GGCCCTACAC CTTTCCCGAT GCTGATGGGG CCTCCAGGCT CCAAGGGAGA CTGTGGCTTG 
CCGGGTCCCC CTGGGCTTCC AGGATTACCT GGATCTCCGG GCGCACGAGG TCCTCGGGGT 
CCTCCTGGGC CGTACGGGAA CCCAGGCCCA CCTGGCCCTC CAGGAGCCAA AGGACAGAAA 
GGGGACCCAG GACTCTCACC AGGACAGGCT CACGATGGAG CAAAGGGCAA CATGGGCTTG 
CCTGGGCTCT CCGGAAATCC AGGACCCTTG GGAAGGAAGG GACACAAGGG CCATCCTGGA 
GCAGCAGGGC ACCCTGGAGA ACAGGGGCAG CCAGGACCTG AGGGCAGTCC AGGGGCCAAA 
GGTTACCCTG GCAGGCAGGG GTTCCCTGGA CCTGTAGGAG ACCCTGGCCC CAAAGGCAGC 
AGGGGTTACA TTGGTCTTCC GGGGCTCTTT GGCCTGCCAG GGTCGGATGG AGAGCGGGGT 
CTCCCTGGCG TTCCTGGCAA GAGGGGCGAG ATGGGTAGGC CGGGCTTCCC TGGAGACTTT 
GGGGAAAGAG GCCCCCCTGG ACTTGATGGC AACCCTGGAG AAATTGGCCT GCCGGGGCCA 
CCAGGAGTGC TCGGGCTCAT TGGTGACACA GGAGCACTGG GCCCAGTTGG CTACCCAGGA 
CCGAAAGGCA TGAAGGGACT GATGGGCGGC GTGGGGGAGC CCGGACTGAA AGGTGATAAG 
GGTGAACAAG GGGTGCCAGG TGTGTCGGGA GATCCTGGCT TCCAAGGAGA CAAGGGGAGT 
CATGGCTTGC CAGGGCTCCC GGGTGGCCGG GGGAAGCCAG GGCCTCTGGG AAAAGCTGGA 
GACAAAGGAT CACTTGGGTT TCCTGGACCT CCTGGACCTG AGGGTTTCCC AGGAGACATT 
GGCCCTCCAG GGGACAATGG CCCTGAAGGC ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG 
CCTGGACCTC CTGGGCAGCT GGGGCCTGAG GGAGATGAAG GACCCATGGG GCCACCAGGA 
GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT 
GGCTCGAAGG GGGAACCAGG GGATCCTGGA CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT 
ATGGGATTCA TTGGTCTTGT CGGAGAGCCT GGAATTGTTG GGGAGAAGGG AGACCGTGGG 
GTGATGGGAC CCCCAGGTGC ACCTGGACCC AAGGGGTCGA TGGGCCATCC TGGAACACCC 
GGTGGTATTG GGAACCCTGG AGAGCCTGGA CCCTGGGGCC CTCCAGGATC TCGAGGCCTG 
CCAGGCATGA GGGGAGCCAA GGGGCACCGG GGCCCTCGAG GACCTGATGG ACCAGCTGGG 
GAGCAGGGAT CCAAGGGCCT GAAGGGTCGT GTAGGACCTC GGGGCAGACC TGGCCAGCCA 
GGACAGCAGG GTGCAGCTGG TGAGCGAGGC CACTCAGGTG CAAAAGGCTT CCTTGGCATC 
CCTGGTCCCT CAGGCCCTCC AGGTGCCAAG GGCCTCCCAG GAGAACCGGG CTCTCAGGGA 
CCTCAGGGGC CAGTTGGTCC TCCAGGAGAA ATGGGGCCCA AGGGACCGCC GGGTGCAGTG 
GGAGAGCCTG GCCTTCCTGG GGACTCCGGA ATGAAGGGTG ACCTCGGACC TCTGGGCCCC 
CCTGGGGAGC AAGGTCTTAT TGGGCAGCGG GGAGAGCCAG GCCTTGAGGG AGACCATGGC 
CCTGTGGGGC CAGATGGGCT GAAGGGCGAC AGGGGCGACC CAGGGCCCGA TGGCGAGCAT 
GGTGAAAAGG GCCAGGAAGG GCTGAAAGGA GAGGATGGGT CCCCTGGGCC CCCGGGCATC 
ACTGGCGTCC CGGGTCGTGA GGGGAAGCCG GGCAAGCAGG GTGAGAAAGG CCAGAGGGGA 
GCTAAGGGTG CCAAGGGCCA CCAAGGATAT CTGGGAGAGA TGGGCATCCC CGGAGAACCT 
GGGCCCCCTG GGACCCCAGG TCCTAAAGGG TCCCGGGGCA CCCTAGGACC AACGGGTGCT 
CCAGGACGGA TGGGAGCCCA AGGAGAACCA GGATTGGCCG GTTATAATGG ACACAAAGGC 
ATCACAGGAC CCCTCGGGCC TCCCGGGCCC AAAGGCGAGA AGGGGGACCA GGGCGAGGAT 
GGCAAGACCG AGGGGCCCCC TGGGCCACCA GGAGATCGGG GTCCTGTGGG TGATCGAGGA 
GACCGTGGGG AGCCAGGCGA CCCTGGATAC CCTGGTCAAG AGGGAGTTCA AGGCCTCCGT 
GGAGAACCGG GCCAGCAGGG ACAGCCTGGG CATCCAGGAC CCCGGGGGCG CCCAGGACCC 
AAAGGATCAA AAGGCGAAGA GGGCCCAAAG GGAAAGCCAG GCAAGGCTGG GCCATCAGGC 
CGGAGGGGGA CCCAGGGCCT TCAAGGACTG CCAGGCCCCC GAGGCGTGGT GGGGAGACAG 
GGCCCTGAGG GCACTGCTGG ATCTGATGGG ATTCCTGGCA GAGATGGTCG GCCAGGATAT 
CAGGGAGACC AGGGAAATGA TGGGGACCCT GGCCCTGTGG GCCCTGCTGG GAGAAGAGGA 
AATCCAGGTG TGGCTGGCTT GCCTGGAGCA CAGGGGCCTC CGGGATTCAA GGGTGAAAGT 
GGGTTACCTG GGCAACTGGG TCCCCCTGGA AAACGAGGGA CAGAAGGTGG AACAGGGCTT 
CCTGGGAACC AGGGGGAGCC AGGATCCAAA GGCCAGCCGG GTGACTCTGG CGAGATGGGC 
TTCCCAGGAG TGGCTGGCCT CTTTGGACCC AAGGGCCCCC CTGGAGACAT TGGCTTCAAA 
GGCATCCAAG GTCCTCGGGG GCCTCCTGGC TTGATGGGAA AGGAAGGTAT CATTGGCCCC 
CCCGGAATGC TGGGACCTTC TGGACTCCCG GGTCCCAAAG GTGACAGAGG CAGCCGAGGA 
GACTTGGGAC TGCAAGGCCC AAGGGGTCCT CCTGGTCCAA GGGGCCGGCC AGGTCCCCCG 
GGCCCTCCTT GGCATCCCAT CCAGTTTCAG CAAGATGACC TTGGAGCAGC TTTCCAGACA 
TGGATGGATG CTCAAGGAGC CGTCAGATCG GAGCAGGGGT ACAGCTATCC GGACCAGCTG 
GCGCTAGACC AGGGAGGGGA GATCTTCAAA ACCTTACACT ACCTCAGCAA CCTCATCCAG 
AGCATTAAGA CACCCTTGGG CACCAAGGAG AACCCAGCCC GGGTCTGCCG GGACCTCATG 
GACTGTGAGC AGAGGATGGC GGATGGTACC TACTGGGTGG ACCCCAACCT CGGCTGCTCC 
TCTGACACCA TTGAAGTCTC CTGCAACTTT ACACAGGGTG GGCAGACGTG CCTGAAGCCC 
ATCACGGCCT CCAAGGCCGA GTTTGCTGTG AGTCGGGTCC AGATGAATTT CTTGCACCTG 
CTGAGCTCTG AGGGGACACA GCATATCACA ATCCACTGTC TGAACATGAC GGTGTGGCAG 
GAGGGACCGG GACGCTCCTC TGCCAGACAG GCTGTGCGCT TCCGTGCCTG GAACGGACAG 
GTCTTCGAAG CTGGGGGTCA GTTCAGGCCA GAGGTGTCTA TGGATGGCTG CAAGGTCCAT 
GATGGCCGCT GGCATCAGAC ACTGTTCACC TTCCGGACCC AGGACCCCCA GCAGCTGCCC 
ATCGTCAGTG TGGACAATCT CCCGCCTGTC TCATCAGGGA AGCAGTACCG CCTGGAAGTT 
GGACCTGCGT GCTTCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006538098.1
CDS	506..6046
Translation	MGTGFARGARGTAASGPGGGFLFAWILVSFTCHLASTQGAPEDVDVLQRLGLSWTKAGGGRSPTPPGVIPFPSGFIFTQRAKLQAPTANVLPTTLGRELALVLSLCSHRVNHAFLFAIRSRKHKLQLGLQFLPGRTIIHLGPRQSVAFDLDVHDGRWHHLALELRGRTVTMVTACGQHRVPVPLPSRRDSMLDPQGSFLLGKVNPRAVQFEGALCQFSIHPVAQVAHNYCAHLRERCRQVDTYSPQVGTLFPWDSGPAFALHPEPALLGLGNLTRTPATLGARPVSRALAVTLAPAMPTKPLRTVHPDVSEHSSSQTPLSPAKQSARKTPSPSSSASLANSTRVYRPAAAQPRQITTTSPTKRSPTKPSVSPLSVTPMKSPHATQKTGVPSFTKPVPPTQKPAPFTSYLAPSKASSPTVRPVQKTFMTPRPPVPSPQPLRPTTGLSKKFTNPTVAKSKSKTTSWASKPVLARSSVPKTLQQTVLSQSPVSYLGSQTLAPALPPLGVGNPRTMPPTRDSALTPAGSKKFTGRETSKKTRQKSSPRKPEPLSPGKSARDASPRDLTTKPSRPSTPALVLAPAYLLSSSPQPTSSSFPFFHLLGPTPFPMLMGPPGSKGDCGLPGPPGLPGLPGSPGARGPRGPPGPYGNPGPPGPPGAKGQKGDPGLSPGQAHDGAKGNMGLPGLSGNPGPLGRKGHKGHPGAAGHPGEQGQPGPEGSPGAKGYPGRQGFPGPVGDPGPKGSRGYIGLPGLFGLPGSDGERGLPGVPGKRGEMGRPGFPGDFGERGPPGLDGNPGEIGLPGPPGVLGLIGDTGALGPVGYPGPKGMKGLMGGVGEPGLKGDKGEQGVPGVSGDPGFQGDKGSHGLPGLPGGRGKPGPLGKAGDKGSLGFPGPPGPEGFPGDIGPPGDNGPEGMKGKPGARGLPGPPGQLGPEGDEGPMGPPGVPGLEGQPGRKGFPGRPGLDGSKGEPGDPGRPGPVGEQGLMGFIGLVGEPGIVGEKGDRGVMGPPGAPGPKGSMGHPGTPGGIGNPGEPGPWGPPGSRGLPGMRGAKGHRGPRGPDGPAGEQGSKGLKGRVGPRGRPGQPGQQGAAGERGHSGAKGFLGIPGPSGPPGAKGLPGEPGSQGPQGPVGPPGEMGPKGPPGAVGEPGLPGDSGMKGDLGPLGPPGEQGLIGQRGEPGLEGDHGPVGPDGLKGDRGDPGPDGEHGEKGQEGLKGEDGSPGPPGITGVPGREGKPGKQGEKGQRGAKGAKGHQGYLGEMGIPGEPGPPGTPGPKGSRGTLGPTGAPGRMGAQGEPGLAGYNGHKGITGPLGPPGPKGEKGDQGEDGKTEGPPGPPGDRGPVGDRGDRGEPGDPGYPGQEGVQGLRGEPGQQGQPGHPGPRGRPGPKGSKGEEGPKGKPGKAGPSGRRGTQGLQGLPGPRGVVGRQGPEGTAGSDGIPGRDGRPGYQGDQGNDGDPGPVGPAGRRGNPGVAGLPGAQGPPGFKGESGLPGQLGPPGKRGTEGGTGLPGNQGEPGSKGQPGDSGEMGFPGVAGLFGPKGPPGDIGFKGIQGPRGPPGLMGKEGIIGPPGMLGPSGLPGPKGDRGSRGDLGLQGPRGPPGPRGRPGPPGPPWHPIQFQQDDLGAAFQTWMDAQGAVRSEQGYSYPDQLALDQGGEIFKTLHYLSNLIQSIKTPLGTKENPARVCRDLMDCEQRMADGTYWVDPNLGCSSDTIEVSCNFTQGGQTCLKPITASKAEFAVSRVQMNFLHLLSSEGTQHITIHCLNMTVWQEGPGRSSARQAVRFRAWNGQVFEAGGQFRPEVSMDGCKVHDGRWHQTLFTFRTQDPQQLPIVSVDNLPPVSSGKQYRLEVGPACFL

Target ORF information:

RefSeq Version	XM_006538035.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006538035.3

ORF Insert Sequence:

ATGGGCACGG GATTCGCGCG GGGGGCCCGA GGCACAGCGG CGTCAGGACC CGGGGGGGGG 
TTTCTCTTCG CCTGGATCTT GGTCTCATTT ACCTGTCACC TGGCCTCCAC CCAAGGAGCT 
CCTGAAGATG TGGATGTCCT CCAGCGGCTG GGCCTCAGCT GGACGAAGGC CGGGGGTGGC 
CGGAGTCCTA CACCCCCTGG TGTCATTCCT TTCCCATCTG GCTTCATCTT CACACAGCGG 
GCCAAGCTGC AGGCCCCCAC AGCCAATGTT CTTCCCACCA CCCTGGGGCG GGAGCTGGCA 
TTGGTGTTGA GCCTCTGTTC ACATCGTGTG AACCATGCCT TCCTCTTTGC CATCCGCAGC 
AGGAAACACA AGCTGCAACT GGGTCTGCAG TTCCTCCCCG GCAGGACGAT CATCCACCTG 
GGGCCTCGGC AATCAGTGGC CTTCGACTTG GATGTGCATG ATGGGCGCTG GCACCACCTG 
GCCCTAGAGT TGCGTGGCCG CACAGTCACA ATGGTGACAG CCTGTGGGCA GCACAGGGTA 
CCTGTCCCAC TGCCTTCCCG TAGGGACTCC ATGCTTGACC CCCAGGGCTC CTTTCTCTTG 
GGGAAGGTGA ACCCCCGTGC TGTCCAGTTT GAAGGTGCAC TCTGTCAGTT CAGCATCCAC 
CCTGTGGCGC AGGTCGCTCA CAATTACTGT GCCCACCTGA GAGAGCGGTG CCGACAGGTG 
GACACATATA GTCCCCAGGT GGGAACCCTC TTTCCTTGGG ACTCTGGCCC AGCCTTTGCT 
TTGCATCCTG AACCAGCCTT GCTTGGCTTG GGGAATCTGA CCAGAACTCC AGCAACCCTG 
GGGGCCAGGC CTGTAAGCAG GGCACTTGCA GTGACTCTGG CTCCTGCCAT GCCCACCAAG 
CCCCTGAGGA CTGTCCACCC AGATGTCTCT GAACACAGCT CTTCCCAGAC CCCACTGTCA 
CCTGCCAAAC AGTCGGCCAG GAAAACACCT TCCCCCTCCT CGTCAGCTTC CCTGGCTAAT 
TCCACCAGGG TTTATCGTCC AGCTGCTGCC CAGCCAAGAC AGATCACAAC TACTAGCCCC 
ACCAAACGTT CCCCCACCAA GCCTTCTGTT TCTCCCCTTT CAGTTACCCC CATGAAAAGT 
CCCCATGCAA CCCAGAAAAC AGGTGTCCCT TCATTCACAA AGCCTGTTCC ACCCACTCAG 
AAGCCAGCGC CCTTCACCTC CTACCTAGCA CCTTCCAAAG CCTCTAGTCC CACTGTGAGG 
CCTGTTCAGA AGACTTTCAT GACACCCCGA CCTCCAGTCC CCAGCCCCCA GCCCCTGCGC 
CCTACTACTG GCTTATCTAA GAAGTTCACT AACCCCACTG TTGCAAAATC TAAGTCCAAG 
ACGACCAGTT GGGCCAGCAA GCCGGTCTTG GCCCGCTCCA GTGTCCCTAA AACGCTTCAG 
CAAACTGTTT TGTCCCAGTC TCCTGTTTCC TATCTGGGCT CTCAGACACT GGCTCCAGCC 
CTCCCTCCTT TGGGTGTTGG AAATCCTAGA ACGATGCCTC CCACACGTGA CTCTGCTTTA 
ACTCCTGCTG GAAGCAAGAA ATTCACAGGA CGGGAAACCT CCAAGAAAAC CAGACAGAAG 
AGCAGCCCCC GGAAGCCCGA ACCTCTCAGC CCTGGGAAGT CAGCCAGGGA TGCCTCACCA 
AGAGACCTGA CAACCAAGCC TAGCCGGCCG TCCACCCCAG CCCTGGTCCT TGCCCCAGCT 
TACCTCCTGT CCTCCAGCCC CCAGCCCACC AGCAGCAGCT TCCCTTTCTT CCACCTGCTG 
GGCCCTACAC CTTTCCCGAT GCTGATGGGG CCTCCAGGCT CCAAGGGAGA CTGTGGCTTG 
CCGGGTCCCC CTGGGCTTCC AGGATTACCT GGATCTCCGG GCGCACGAGG TCCTCGGGGT 
CCTCCTGGGC CGTACGGGAA CCCAGGCCCA CCTGGCCCTC CAGGAGCCAA AGGACAGAAA 
GGGGACCCAG GACTCTCACC AGGACAGGCT CACGATGGAG CAAAGGGCAA CATGGGCTTG 
CCTGGGCTCT CCGGAAATCC AGGACCCTTG GGAAGGAAGG GACACAAGGG CCATCCTGGA 
GCAGCAGGGC ACCCTGGAGA ACAGGGGCAG CCAGGACCTG AGGGCAGTCC AGGGGCCAAA 
GGTTACCCTG GCAGGCAGGG GTTCCCTGGA CCTGTAGGAG ACCCTGGCCC CAAAGGCAGC 
AGGGGTTACA TTGGTCTTCC GGGGCTCTTT GGCCTGCCAG GGTCGGATGG AGAGCGGGGT 
CTCCCTGGCG TTCCTGGCAA GAGGGGCGAG ATGGGTAGGC CGGGCTTCCC TGGAGACTTT 
GGGGAAAGAG GCCCCCCTGG ACTTGATGGC AACCCTGGAG AAATTGGCCT GCCGGGGCCA 
CCAGGAGTGC TCGGGCTCAT TGGTGACACA GGAGCACTGG GCCCAGTTGG CTACCCAGGA 
CCGAAAGGCA TGAAGGGACT GATGGGCGGC GTGGGGGAGC CCGGACTGAA AGGTGATAAG 
GGTGAACAAG GGGTGCCAGG TGTGTCGGGA GATCCTGGCT TCCAAGGAGA CAAGGGGAGT 
CATGGCTTGC CAGGGCTCCC GGGTGGCCGG GGGAAGCCAG GGCCTCTGGG AAAAGCTGGA 
GACAAAGGAT CACTTGGGTT TCCTGGACCT CCTGGACCTG AGGGTTTCCC AGGAGACATT 
GGCCCTCCAG GGGACAATGG CCCTGAAGGC ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG 
CCTGGACCTC CTGGGCAGCT GGGGCCTGAG GGAGATGAAG GACCCATGGG GCCACCAGGA 
GTCCCAGGCT TGGAGGGTCA GCCTGGAAGG AAAGGGTTTC CTGGGAGGCC TGGCCTGGAT 
GGCTCGAAGG GGGAACCAGG GGATCCTGGA CGGCCAGGAC CTGTGGGTGA ACAGGGGCTT 
ATGGGATTCA TTGGTCTTGT CGGAGAGCCT GGAATTGTTG GGGAGAAGGG AGACCGTGGG 
GTGATGGGAC CCCCAGGTGC ACCTGGACCC AAGGGGTCGA TGGGCCATCC TGGAACACCC 
GGTGGTATTG GGAACCCTGG AGAGCCTGGA CCCTGGGGCC CTCCAGGATC TCGAGGCCTG 
CCAGGCATGA GGGGAGCCAA GGGGCACCGG GGCCCTCGAG GACCTGATGG ACCAGCTGGG 
GAGCAGGGAT CCAAGGGCCT GAAGGGTCGT GTAGGACCTC GGGGCAGACC TGGCCAGCCA 
GGACAGCAGG GTGCAGCTGG TGAGCGAGGC CACTCAGGTG CAAAAGGCTT CCTTGGCATC 
CCTGGTCCCT CAGGCCCTCC AGGTGCCAAG GGCCTCCCAG GAGAACCGGG CTCTCAGGGA 
CCTCAGGGGC CAGTTGGTCC TCCAGGAGAA ATGGGGCCCA AGGGACCGCC GGGTGCAGTG 
GGAGAGCCTG GCCTTCCTGG GGACTCCGGA ATGAAGGGTG ACCTCGGACC TCTGGGCCCC 
CCTGGGGAGC AAGGTCTTAT TGGGCAGCGG GGAGAGCCAG GCCTTGAGGG AGACCATGGC 
CCTGTGGGGC CAGATGGGCT GAAGGGCGAC AGGGGCGACC CAGGGCCCGA TGGCGAGCAT 
GGTGAAAAGG GCCAGGAAGG GCTGAAAGGA GAGGATGGGT CCCCTGGGCC CCCGGGCATC 
ACTGGCGTCC CGGGTCGTGA GGGGAAGCCG GGCAAGCAGG GTGAGAAAGG CCAGAGGGGA 
GCTAAGGGTG CCAAGGGCCA CCAAGGATAT CTGGGAGAGA TGGGCATCCC CGGAGAACCT 
GGGCCCCCTG GGACCCCAGG TCCTAAAGGG TCCCGGGGCA CCCTAGGACC AACGGGTGCT 
CCAGGACGGA TGGGAGCCCA AGGAGAACCA GGATTGGCCG GTTATAATGG ACACAAAGGC 
ATCACAGGAC CCCTCGGGCC TCCCGGGCCC AAAGGCGAGA AGGGGGACCA GGGCGAGGAT 
GGCAAGACCG AGGGGCCCCC TGGGCCACCA GGAGATCGGG GTCCTGTGGG TGATCGAGGA 
GACCGTGGGG AGCCAGGCGA CCCTGGATAC CCTGGTCAAG AGGGAGTTCA AGGCCTCCGT 
GGAGAACCGG GCCAGCAGGG ACAGCCTGGG CATCCAGGAC CCCGGGGGCG CCCAGGACCC 
AAAGGATCAA AAGGCGAAGA GGGCCCAAAG GGAAAGCCAG GCAAGGCTGG GCCATCAGGC 
CGGAGGGGGA CCCAGGGCCT TCAAGGACTG CCAGGCCCCC GAGGCGTGGT GGGGAGACAG 
GGCCCTGAGG GCACTGCTGG ATCTGATGGG ATTCCTGGCA GAGATGGTCG GCCAGGATAT 
CAGGGAGACC AGGGAAATGA TGGGGACCCT GGCCCTGTGG GCCCTGCTGG GAGAAGAGGA 
AATCCAGGTG TGGCTGGCTT GCCTGGAGCA CAGGGGCCTC CGGGATTCAA GGGTGAAAGT 
GGGTTACCTG GGCAACTGGG TCCCCCTGGA AAACGAGGGA CAGAAGGTGG AACAGGGCTT 
CCTGGGAACC AGGGGGAGCC AGGATCCAAA GGCCAGCCGG GTGACTCTGG CGAGATGGGC 
TTCCCAGGAG TGGCTGGCCT CTTTGGACCC AAGGGCCCCC CTGGAGACAT TGGCTTCAAA 
GGCATCCAAG GTCCTCGGGG GCCTCCTGGC TTGATGGGAA AGGAAGGTAT CATTGGCCCC 
CCCGGAATGC TGGGACCTTC TGGACTCCCG GGTCCCAAAG GTGACAGAGG CAGCCGAGGA 
GACTTGGGAC TGCAAGGCCC AAGGGGTCCT CCTGGTCCAA GGGGCCGGCC AGGTCCCCCG 
GGCCCTCCTT GGCATCCCAT CCAGTTTCAG CAAGATGACC TTGGAGCAGC TTTCCAGACA 
TGGATGGATG CTCAAGGAGC CGTCAGATCG GAGCAGGGGT ACAGCTATCC GGACCAGCTG 
GCGCTAGACC AGGGAGGGGA GATCTTCAAA ACCTTACACT ACCTCAGCAA CCTCATCCAG 
AGCATTAAGA CACCCTTGGG CACCAAGGAG AACCCAGCCC GGGTCTGCCG GGACCTCATG 
GACTGTGAGC AGAGGATGGC GGATGGTACC TACTGGGTGG ACCCCAACCT CGGCTGCTCC 
TCTGACACCA TTGAAGTCTC CTGCAACTTT ACACAGGGTG GGCAGACGTG CCTGAAGCCC 
ATCACGGCCT CCAAGGCCGA GTTTGCTGTG AGTCGGGTCC AGATGAATTT CTTGCACCTG 
CTGAGCTCTG AGGGGACACA GCATATCACA ATCCACTGTC TGAACATGAC GGTGTGGCAG 
GAGGGACCGG GACGCTCCTC TGCCAGACAG GCTGTGCGCT TCCGTGCCTG GAACGGACAG 
GTCTTCGAAG CTGGGGGTCA GTTCAGGCCA GAGGTGTCTA TGGATGGCTG CAAGGTCCAT 
GATGGCCGCT GGCATCAGAC ACTGTTCACC TTCCGGACCC AGGACCCCCA GCAGCTGCCC 
ATCGTCAGTG TGGACAATCT CCCGCCTGTC TCATCAGGGA AGCAGTACCG CCTGGAAGTT 
GGACCTGCGT GCTTCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Col27a1
Entrez Gene ID	373864
Full Name	collagen, type XXVII, alpha 1
Synonyms	5730512J02Rik,AI449266
General protein information	Preferred Names collagen, type XXVII, alpha 1 Names collagen alpha-1(XXVII) chain procollagen, type XXVII, alpha 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4\|4 B3
Summary	This gene encodes the alpha-1 subunit of type XXVII collagen, one of the low abundance fibril-forming collagens found in cartilage. The encoded protein forms a homotrimeric triple helical procollagen that undergoes proteolytic processing during fibril formation. Transgenic mice lacking a portion of the collagenous domain in the encoded protein exhibit skeletal abnormalities, chondrodysplasia and die at birth because of a lung defect. [provided by RefSeq, Dec 2015].

Bos taurus (cattle)	COL27A1	NP_001193609.1
Rattus norvegicus (Norway rat)	Col27a1	NP_942042.1
Homo sapiens (human)	COL27A1	NP_116277.2
Canis lupus familiaris (dog)	COL27A1	XP_005627059.1
Pan troglodytes (chimpanzee)	COL27A1	XP_520208.3
Gallus gallus (chicken)	COL27A1	XP_415514.3
Mus musculus (house mouse)	Col27a1	NP_079961.3
Xenopus tropicalis (tropical clawed frog)	LOC100490770	XP_004916755.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Col27a1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

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